Chst5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chst5
Entrez Gene ID	56773
Full Name	carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5
Synonyms	AI173964,GST-4,Gn6st-3,I-GlcNAc-6-ST
General protein information	Preferred Names carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5 Names carbohydrate sulfotransferase 5 GST4 I-GlcNAc6ST N-acetylglucosamine 6-O-sulfotransferase 3 galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4 glcNAc6ST-3 intestinal GlcNAc-6-sulfotransferase intestinal N-acetylglucosamine-6-O-sulfotransferase mIGn6ST
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_019950.2	NP_064334.1	carbohydrate sulfotransferase 5 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHST6	NP_067628.1
Canis lupus familiaris (dog)	CHST6	XP_005620746.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009809	XP_318914.4
Pan troglodytes (chimpanzee)	CHST6	XP_001141466.1
Rattus norvegicus (Norway rat)	Chst5	NP_001100901.1
Drosophila melanogaster (fruit fly)	CG31637	NP_609043.1
	CHST6	XP_001110474.1
Bos taurus (cattle)	CHST6	NP_001178951.1
Gallus gallus (chicken)	CHST6	XP_004944417.1
Danio rerio (zebrafish)	zgc:194879	NP_001124093.1
Mus musculus (house mouse)	Chst5	NP_064334.1
Xenopus tropicalis (tropical clawed frog)	chst6	XP_004913666.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Electron tomography reveals multiple self-association of chondroitin sulphate/dermatan sulphate proteoglycans in Chst5-null mouse corneas.
Parfitt GJ, Pinali C, Akama TO, Young RD, Nishida K, Quantock AJ, Knupp C
Journal of structural biology174(3)536-41(2011 Jun)

Matrix morphogenesis in cornea is mediated by the modification of keratan sulfate by GlcNAc 6-O-sulfotransferase.
Hayashida Y, Akama TO, Beecher N, Lewis P, Young RD, Meek KM, Kerr B, Hughes CE, Caterson B, Tanigami A, Nakayama J, Fukada MN, Tano Y, Nishida K, Quantock AJ
Proceedings of the National Academy of Sciences of the United States of America103(36)13333-8(2006 Sep)

Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate.
Akama TO, Misra AK, Hindsgaul O, Fukuda MN
The Journal of biological chemistry277(45)42505-13(2002 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that in Chst5-KO mice, multiple chondroitin sulphate/dermatan sulphate proteoglycans have aggregated laterally and, possibly, end-to-end, with orthogonal extensions protruding from the main electron-dense stained filament.
Title: Electron tomography reveals multiple self-association of chondroitin sulphate/dermatan sulphate proteoglycans in Chst5-null mouse corneas.

The following Chst5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chst5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11909	NM_019950.2 Latest version!	Mus musculus carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5 (Chst5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chst5 ( NM_019950.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064334.1 Mus musculus carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5 (Chst5), mRNA.

Price & Availability↑

CloneID	OMu11909
Clone ID Related Accession (Same CDS sequence)	NM_019950.2
Accession Version	NM_019950.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-14
Organism	Mus musculus(house mouse)
Product	carbohydrate sulfotransferase 5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF176840.1 and AC127315.4. On Jul 28, 2006 this sequence version replaced NM_019950.1. ##Evidence-Data-START## Transcript exon combination :: BY711808.1, AI115260.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCTAC CCCGTTTCTC CAGCACTGTC ATGCTTTCGC TCCTGATGGT ACAGACTGGC 
ATCCTGGTCT TCCTGGTCTC CCGGCAAGTG CCATCGTCCC CAGCAGGCCT TGGGGAGCGT 
GTGCACGTGC TGGTACTGTC CTCGTGGCGC TCGGGCTCGT CCTTCGTGGG CCAGCTCTTC 
AGCCAACACC CCGATGTCTT CTACCTGATG GAGCCGGCTT GGCACGTCTG GGATACGTTG 
TCGCAGGGCA GTGCCCCCGC ACTCCACATG GCCGTGCGTG ACCTGATCCG CTCAGTGTTC 
CTATGCGACA TGGACGTATT TGATGCCTAC CTGCCCTGGC GCCGCAACAT CTCGGATCTC 
TTCCAGTGGG CGGTGAGCCG CGCATTGTGC TCACCTCCGG TCTGCGAAGC CTTCGCTCGT 
GGCAACATCA GCAGCGAGGA GGTGTGTAAG CCTCTGTGCG CAACGCGGCC CTTCGGCCTG 
GCTCAGGAAG CCTGCAGCTC CTATAGTCAC GTCGTGCTCA AGGAGGTGCG CTTCTTTAAC 
CTACAGGTGC TCTACCCGCT GCTCAGCGAC CCTGCGCTCA ACCTGCGCAT CGTGCACCTA 
GTGCGCGACC CGCGGGCCGT GCTGCGCTCC CGAGAGCAGA CAGCCAAGGC GCTGGCACGG 
GACAATGGCA TCGTCCTGGG TACCAACGGC ACGTGGGTGG AGGCGGACCC CCGGCTGCGC 
GTGGTCAACG AGGTATGCCG CAGCCATGTG CGCATCGCAG AGGCAGCCTT GCACAAGCCG 
CCGCCCTTCT TGCAAGATCG CTACCGCCTG GTGCGCTACG AGGATCTGGC CCGGGACCCA 
CTCACCGTAA TCCGTGAACT CTATGCCTTC ACCGGCCTGG GTCTCACGCC GCAGCTCCAG 
ACTTGGATCC ACAATATCAC GCATGGTTCA GGGCCAGGCG CGCGCCGTGA AGCCTTCAAG 
ACCACATCCA GGGATGCGCT CAGTGTATCC CAGGCCTGGC GCCACACGCT GCCCTTTGCC 
AAGATTCGCC GGGTCCAGGA ACTGTGCGGG GGTGCACTGC AGCTGCTGGG TTACCGGTCT 
GTGCATTCGG AGCTTGAGCA AAGGGACCTC TCTCTGGACC TCCTGCTGCC AAGAGGCATG 
GACAGTTTCA AGTGGGCATC GTCCACGGAG AAGCAACCGG AATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064334.1
CDS	84..1271
Translation	MRLPRFSSTVMLSLLMVQTGILVFLVSRQVPSSPAGLGERVHVLVLSSWRSGSSFVGQLFSQHPDVFYLMEPAWHVWDTLSQGSAPALHMAVRDLIRSVFLCDMDVFDAYLPWRRNISDLFQWAVSRALCSPPVCEAFARGNISSEEVCKPLCATRPFGLAQEACSSYSHVVLKEVRFFNLQVLYPLLSDPALNLRIVHLVRDPRAVLRSREQTAKALARDNGIVLGTNGTWVEADPRLRVVNEVCRSHVRIAEAALHKPPPFLQDRYRLVRYEDLARDPLTVIRELYAFTGLGLTPQLQTWIHNITHGSGPGARREAFKTTSRDALSVSQAWRHTLPFAKIRRVQELCGGALQLLGYRSVHSELEQRDLSLDLLLPRGMDSFKWASSTEKQPES

Target ORF information:

RefSeq Version	NM_019950.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5 (Chst5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019950.2

ORF Insert Sequence:

ATGCGGCTAC CCCGTTTCTC CAGCACTGTC ATGCTTTCGC TCCTGATGGT ACAGACTGGC 
ATCCTGGTCT TCCTGGTCTC CCGGCAAGTG CCATCGTCCC CAGCAGGCCT TGGGGAGCGT 
GTGCACGTGC TGGTACTGTC CTCGTGGCGC TCGGGCTCGT CCTTCGTGGG CCAGCTCTTC 
AGCCAACACC CCGATGTCTT CTACCTGATG GAGCCGGCTT GGCACGTCTG GGATACGTTG 
TCGCAGGGCA GTGCCCCCGC ACTCCACATG GCCGTGCGTG ACCTGATCCG CTCAGTGTTC 
CTATGCGACA TGGACGTATT TGATGCCTAC CTGCCCTGGC GCCGCAACAT CTCGGATCTC 
TTCCAGTGGG CGGTGAGCCG CGCATTGTGC TCACCTCCGG TCTGCGAAGC CTTCGCTCGT 
GGCAACATCA GCAGCGAGGA GGTGTGTAAG CCTCTGTGCG CAACGCGGCC CTTCGGCCTG 
GCTCAGGAAG CCTGCAGCTC CTATAGTCAC GTCGTGCTCA AGGAGGTGCG CTTCTTTAAC 
CTACAGGTGC TCTACCCGCT GCTCAGCGAC CCTGCGCTCA ACCTGCGCAT CGTGCACCTA 
GTGCGCGACC CGCGGGCCGT GCTGCGCTCC CGAGAGCAGA CAGCCAAGGC GCTGGCACGG 
GACAATGGCA TCGTCCTGGG TACCAACGGC ACGTGGGTGG AGGCGGACCC CCGGCTGCGC 
GTGGTCAACG AGGTATGCCG CAGCCATGTG CGCATCGCAG AGGCAGCCTT GCACAAGCCG 
CCGCCCTTCT TGCAAGATCG CTACCGCCTG GTGCGCTACG AGGATCTGGC CCGGGACCCA 
CTCACCGTAA TCCGTGAACT CTATGCCTTC ACCGGCCTGG GTCTCACGCC GCAGCTCCAG 
ACTTGGATCC ACAATATCAC GCATGGTTCA GGGCCAGGCG CGCGCCGTGA AGCCTTCAAG 
ACCACATCCA GGGATGCGCT CAGTGTATCC CAGGCCTGGC GCCACACGCT GCCCTTTGCC 
AAGATTCGCC GGGTCCAGGA ACTGTGCGGG GGTGCACTGC AGCTGCTGGG TTACCGGTCT 
GTGCATTCGG AGCTTGAGCA AAGGGACCTC TCTCTGGACC TCCTGCTGCC AAGAGGCATG 
GACAGTTTCA AGTGGGCATC GTCCACGGAG AAGCAACCGG AATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Electron tomography reveals multiple self-association of chondroitin sulphate/dermatan sulphate proteoglycans in Chst5-null mouse corneas.
Journal of structural biology174(3)536-41(2011 Jun)
Parfitt GJ,Pinali C,Akama TO,Young RD,Nishida K,Quantock AJ,Knupp C

Matrix morphogenesis in cornea is mediated by the modification of keratan sulfate by GlcNAc 6-O-sulfotransferase.
Proceedings of the National Academy of Sciences of the United States of America103(36)13333-8(2006 Sep)
Hayashida Y,Akama TO,Beecher N,Lewis P,Young RD,Meek KM,Kerr B,Hughes CE,Caterson B,Tanigami A,Nakayama J,Fukada MN,Tano Y,Nishida K,Quantock AJ

Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate.
The Journal of biological chemistry277(45)42505-13(2002 Nov)
Akama TO,Misra AK,Hindsgaul O,Fukuda MN

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Chst5 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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