Clcf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clcf1
Entrez Gene ID	56708
Full Name	cardiotrophin-like cytokine factor 1
Synonyms	BSF-3,Bsf3,CLC,NNT-1
General protein information	Preferred Names cardiotrophin-like cytokine factor 1 Names cardiotrophin-like cytokine factor 1 B-cell stimulating factor 3 neurotrophin-1/B-cell stimulating factor-3 novel neurotrophin-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
NM_001310038.2	NP_001296967.1	cardiotrophin-like cytokine factor 1 isoform 2 precursor
NM_001310039.1	NP_001296968.1	cardiotrophin-like cytokine factor 1 isoform 2 precursor
NM_019952.5	NP_064336.1	cardiotrophin-like cytokine factor 1 isoform 1 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Clcf1	NP_997498.2
Danio rerio (zebrafish)	LOC562247	XP_690733.5
Xenopus tropicalis (tropical clawed frog)	clcf1	NP_001016067.1
Bos taurus (cattle)	CLCF1	XP_002699410.1
	CLCF1	XP_001106809.1
Gallus gallus (chicken)	CLCF1	XP_003643835.2
Homo sapiens (human)	CLCF1	NP_037378.1
Canis lupus familiaris (dog)	CLCF1	XP_540818.2
Mus musculus (house mouse)	Clcf1	NP_064336.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders.
Sims NA
Cytokine & growth factor reviews26(5)517-22(2015 Oct)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

DJ-1 enhances cell survival through the binding of Cezanne, a negative regulator of NF-kappaB.
McNally RS, Davis BK, Clements CM, Accavitti-Loper MA, Mak TW, Ting JP
The Journal of biological chemistry286(6)4098-106(2011 Feb)

Ciliary neurotrophic factor inhibits bone formation and plays a sex-specific role in bone growth and remodeling.
McGregor NE, Poulton IJ, Walker EC, Pompolo S, Quinn JM, Martin TJ, Sims NA
Calcified tissue international86(3)261-70(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders.
Title: Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders.

In this study, Clc knockout mice had decreased facial motility, did not suckle, died within 24 hours, and had 32% and 29% fewer motor neurons in the facial nucleus and lumbar ventral horn, respectively.
Title: Neonatal death in mice lacking cardiotrophin-like cytokine is associated with multifocal neuronal hypoplasia.

IL-6 (gp130) family cytokine members include CLC/NNT1/BSF3 and IL-27 [review]
Title: New IL-6 (gp130) family cytokine members, CLC/NNT1/BSF3 and IL-27.

neurotrophin-1/B-cell stimulating factor 3(NNT-1/BSF-3) is expressed in pituitary folliculostellate TtT/GF cells and induced by PKC-, PKA-, and ERK1/2-dependent mechanisms
Title: Expression of novel neurotrophin-1/B-cell stimulating factor-3 (NNT-1/BSF-3) in murine pituitary folliculostellate TtT/GF cells: pituitary adenylate cyclase-activating polypeptide and vasoactive intestinal peptide-induced stimulation of NNT-1/BSF-3 is mediated by protein kinase A, protein kinase C, and extracellular-signal-regulated kinase1/2 pathways.

CLC plays a role in astrocyte differentiation via STAT3 activation within the developing brain
Title: Cardiotrophin-like cytokine induces astrocyte differentiation of fetal neuroepithelial cells via activation of STAT3.

The following Clcf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clcf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69677	NM_001310038.2 Latest version!	Mus musculus cardiotrophin-like cytokine factor 1 (Clcf1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu69677	NM_001310039.1 Latest version!	Mus musculus cardiotrophin-like cytokine factor 1 (Clcf1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu11833	NM_019952.5 Latest version!	Mus musculus cardiotrophin-like cytokine factor 1 (Clcf1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clcf1 ( NM_001310038.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001296967.1 Mus musculus cardiotrophin-like cytokine factor 1 (Clcf1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69677
Clone ID Related Accession (Same CDS sequence)	NM_001310038.2 , NM_001310039.1
Accession Version	NM_001310038.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	cardiotrophin-like cytokine factor 1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109138.10, AK137396.1, BC104258.1, CX202966.1, BB786146.1 and AI451696.1. On Jun 25, 2015 this sequence version replaced NM_001310038.1. Transcript Variant: This variant (2) contains an alternate exon in the 5' region resulting in differences in the 5' UTR and translation initiation from a downstream start codon, compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1. Both variants 2 and 3 encode the same protein (isoform 2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK166218.1, BY734701.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTAGCTT GCCTATGCAC GGTGCTGTGG CACCTCCCTG CAGTGCCAGC TCTTAATCGC 
ACAGGAGATC CAGGCCCTGG CCCCTCCATC CAGAAAACCT ATGACCTCAC CCGCTACCTG 
GAGCATCAAC TCCGCAGCTT AGCTGGGACC TACCTGAACT ACCTGGGGCC CCCTTTCAAC 
GAGCCTGACT TCAATCCTCC TCGACTGGGG GCAGAAACTC TGCCCAGGGC CACGGTCAAC 
TTGGAAGTGT GGCGAAGCCT CAATGACAGG CTGCGGCTGA CCCAGAACTA TGAGGCGTAC 
AGTCACCTCC TGTGTTACTT GCGTGGCCTC AACCGTCAGG CTGCCACAGC TGAACTCCGA 
CGTAGCCTGG CCCACTTCTG TACCAGCCTC CAGGGCCTGC TGGGCAGCAT TGCAGGTGTC 
ATGGCGACGC TTGGCTACCC ACTGCCCCAG CCTCTGCCAG GGACTGAGCC AGCCTGGGCC 
CCTGGCCCTG CCCACAGTGA CTTCCTCCAG AAGATGGATG ACTTCTGGCT GCTGAAGGAG 
CTGCAGACCT GGCTATGGCG TTCAGCCAAG GACTTCAACC GGCTTAAGAA GAAGATGCAG 
CCTCCAGCAG CTTCAGTCAC CCTGCACTTG GAGGCCCATG GTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296967.1
CDS	376..1023
Translation	MLACLCTVLWHLPAVPALNRTGDPGPGPSIQKTYDLTRYLEHQLRSLAGTYLNYLGPPFNEPDFNPPRLGAETLPRATVNLEVWRSLNDRLRLTQNYEAYSHLLCYLRGLNRQAATAELRRSLAHFCTSLQGLLGSIAGVMATLGYPLPQPLPGTEPAWAPGPAHSDFLQKMDDFWLLKELQTWLWRSAKDFNRLKKKMQPPAASVTLHLEAHGF

Target ORF information:

RefSeq Version	NM_001310038.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cardiotrophin-like cytokine factor 1 (Clcf1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310038.2

ORF Insert Sequence:

ATGTTAGCTT GCCTATGCAC GGTGCTGTGG CACCTCCCTG CAGTGCCAGC TCTTAATCGC 
ACAGGAGATC CAGGCCCTGG CCCCTCCATC CAGAAAACCT ATGACCTCAC CCGCTACCTG 
GAGCATCAAC TCCGCAGCTT AGCTGGGACC TACCTGAACT ACCTGGGGCC CCCTTTCAAC 
GAGCCTGACT TCAATCCTCC TCGACTGGGG GCAGAAACTC TGCCCAGGGC CACGGTCAAC 
TTGGAAGTGT GGCGAAGCCT CAATGACAGG CTGCGGCTGA CCCAGAACTA TGAGGCGTAC 
AGTCACCTCC TGTGTTACTT GCGTGGCCTC AACCGTCAGG CTGCCACAGC TGAACTCCGA 
CGTAGCCTGG CCCACTTCTG TACCAGCCTC CAGGGCCTGC TGGGCAGCAT TGCAGGTGTC 
ATGGCGACGC TTGGCTACCC ACTGCCCCAG CCTCTGCCAG GGACTGAGCC AGCCTGGGCC 
CCTGGCCCTG CCCACAGTGA CTTCCTCCAG AAGATGGATG ACTTCTGGCT GCTGAAGGAG 
CTGCAGACCT GGCTATGGCG TTCAGCCAAG GACTTCAACC GGCTTAAGAA GAAGATGCAG 
CCTCCAGCAG CTTCAGTCAC CCTGCACTTG GAGGCCCATG GTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcf1 ( NM_001310039.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001296968.1 Mus musculus cardiotrophin-like cytokine factor 1 (Clcf1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu69677
Clone ID Related Accession (Same CDS sequence)	NM_001310038.2 , NM_001310039.1
Accession Version	NM_001310039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	cardiotrophin-like cytokine factor 1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109138.10, CX224627.1, BC104258.1, CX202966.1, BB786146.1 and AI451696.1. Transcript Variant: This variant (3) contains an alternate 5' terminal exon resulting in differences in the 5' UTR and translation initiation from a downstream start codon, compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1. Both variants 2 and 3 encode the same protein (isoform 2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CX224627.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTAGCTT GCCTATGCAC GGTGCTGTGG CACCTCCCTG CAGTGCCAGC TCTTAATCGC 
ACAGGAGATC CAGGCCCTGG CCCCTCCATC CAGAAAACCT ATGACCTCAC CCGCTACCTG 
GAGCATCAAC TCCGCAGCTT AGCTGGGACC TACCTGAACT ACCTGGGGCC CCCTTTCAAC 
GAGCCTGACT TCAATCCTCC TCGACTGGGG GCAGAAACTC TGCCCAGGGC CACGGTCAAC 
TTGGAAGTGT GGCGAAGCCT CAATGACAGG CTGCGGCTGA CCCAGAACTA TGAGGCGTAC 
AGTCACCTCC TGTGTTACTT GCGTGGCCTC AACCGTCAGG CTGCCACAGC TGAACTCCGA 
CGTAGCCTGG CCCACTTCTG TACCAGCCTC CAGGGCCTGC TGGGCAGCAT TGCAGGTGTC 
ATGGCGACGC TTGGCTACCC ACTGCCCCAG CCTCTGCCAG GGACTGAGCC AGCCTGGGCC 
CCTGGCCCTG CCCACAGTGA CTTCCTCCAG AAGATGGATG ACTTCTGGCT GCTGAAGGAG 
CTGCAGACCT GGCTATGGCG TTCAGCCAAG GACTTCAACC GGCTTAAGAA GAAGATGCAG 
CCTCCAGCAG CTTCAGTCAC CCTGCACTTG GAGGCCCATG GTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296968.1
CDS	259..906
Translation	MLACLCTVLWHLPAVPALNRTGDPGPGPSIQKTYDLTRYLEHQLRSLAGTYLNYLGPPFNEPDFNPPRLGAETLPRATVNLEVWRSLNDRLRLTQNYEAYSHLLCYLRGLNRQAATAELRRSLAHFCTSLQGLLGSIAGVMATLGYPLPQPLPGTEPAWAPGPAHSDFLQKMDDFWLLKELQTWLWRSAKDFNRLKKKMQPPAASVTLHLEAHGF

Target ORF information:

RefSeq Version	NM_001310039.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cardiotrophin-like cytokine factor 1 (Clcf1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310039.1

ORF Insert Sequence:

ATGTTAGCTT GCCTATGCAC GGTGCTGTGG CACCTCCCTG CAGTGCCAGC TCTTAATCGC 
ACAGGAGATC CAGGCCCTGG CCCCTCCATC CAGAAAACCT ATGACCTCAC CCGCTACCTG 
GAGCATCAAC TCCGCAGCTT AGCTGGGACC TACCTGAACT ACCTGGGGCC CCCTTTCAAC 
GAGCCTGACT TCAATCCTCC TCGACTGGGG GCAGAAACTC TGCCCAGGGC CACGGTCAAC 
TTGGAAGTGT GGCGAAGCCT CAATGACAGG CTGCGGCTGA CCCAGAACTA TGAGGCGTAC 
AGTCACCTCC TGTGTTACTT GCGTGGCCTC AACCGTCAGG CTGCCACAGC TGAACTCCGA 
CGTAGCCTGG CCCACTTCTG TACCAGCCTC CAGGGCCTGC TGGGCAGCAT TGCAGGTGTC 
ATGGCGACGC TTGGCTACCC ACTGCCCCAG CCTCTGCCAG GGACTGAGCC AGCCTGGGCC 
CCTGGCCCTG CCCACAGTGA CTTCCTCCAG AAGATGGATG ACTTCTGGCT GCTGAAGGAG 
CTGCAGACCT GGCTATGGCG TTCAGCCAAG GACTTCAACC GGCTTAAGAA GAAGATGCAG 
CCTCCAGCAG CTTCAGTCAC CCTGCACTTG GAGGCCCATG GTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcf1 ( NM_019952.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064336.1 Mus musculus cardiotrophin-like cytokine factor 1 (Clcf1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11833
Clone ID Related Accession (Same CDS sequence)	NM_019952.5
Accession Version	NM_019952.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	cardiotrophin-like cytokine factor 1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109138.10, AK137396.1, BC104258.1, CX202966.1, BB786146.1 and AI451696.1. On Jun 25, 2015 this sequence version replaced NM_019952.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK166421.1, AF176913.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCTCC GAGCAGGGGA CTCGTGGGGG ATGTTAGCTT GCCTATGCAC GGTGCTGTGG 
CACCTCCCTG CAGTGCCAGC TCTTAATCGC ACAGGAGATC CAGGCCCTGG CCCCTCCATC 
CAGAAAACCT ATGACCTCAC CCGCTACCTG GAGCATCAAC TCCGCAGCTT AGCTGGGACC 
TACCTGAACT ACCTGGGGCC CCCTTTCAAC GAGCCTGACT TCAATCCTCC TCGACTGGGG 
GCAGAAACTC TGCCCAGGGC CACGGTCAAC TTGGAAGTGT GGCGAAGCCT CAATGACAGG 
CTGCGGCTGA CCCAGAACTA TGAGGCGTAC AGTCACCTCC TGTGTTACTT GCGTGGCCTC 
AACCGTCAGG CTGCCACAGC TGAACTCCGA CGTAGCCTGG CCCACTTCTG TACCAGCCTC 
CAGGGCCTGC TGGGCAGCAT TGCAGGTGTC ATGGCGACGC TTGGCTACCC ACTGCCCCAG 
CCTCTGCCAG GGACTGAGCC AGCCTGGGCC CCTGGCCCTG CCCACAGTGA CTTCCTCCAG 
AAGATGGATG ACTTCTGGCT GCTGAAGGAG CTGCAGACCT GGCTATGGCG TTCAGCCAAG 
GACTTCAACC GGCTTAAGAA GAAGATGCAG CCTCCAGCAG CTTCAGTCAC CCTGCACTTG 
GAGGCCCATG GTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064336.1
CDS	248..925
Translation	MDLRAGDSWGMLACLCTVLWHLPAVPALNRTGDPGPGPSIQKTYDLTRYLEHQLRSLAGTYLNYLGPPFNEPDFNPPRLGAETLPRATVNLEVWRSLNDRLRLTQNYEAYSHLLCYLRGLNRQAATAELRRSLAHFCTSLQGLLGSIAGVMATLGYPLPQPLPGTEPAWAPGPAHSDFLQKMDDFWLLKELQTWLWRSAKDFNRLKKKMQPPAASVTLHLEAHGF

Target ORF information:

RefSeq Version	NM_019952.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus cardiotrophin-like cytokine factor 1 (Clcf1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019952.5

ORF Insert Sequence:

ATGGACCTCC GAGCAGGGGA CTCGTGGGGG ATGTTAGCTT GCCTATGCAC GGTGCTGTGG 
CACCTCCCTG CAGTGCCAGC TCTTAATCGC ACAGGAGATC CAGGCCCTGG CCCCTCCATC 
CAGAAAACCT ATGACCTCAC CCGCTACCTG GAGCATCAAC TCCGCAGCTT AGCTGGGACC 
TACCTGAACT ACCTGGGGCC CCCTTTCAAC GAGCCTGACT TCAATCCTCC TCGACTGGGG 
GCAGAAACTC TGCCCAGGGC CACGGTCAAC TTGGAAGTGT GGCGAAGCCT CAATGACAGG 
CTGCGGCTGA CCCAGAACTA TGAGGCGTAC AGTCACCTCC TGTGTTACTT GCGTGGCCTC 
AACCGTCAGG CTGCCACAGC TGAACTCCGA CGTAGCCTGG CCCACTTCTG TACCAGCCTC 
CAGGGCCTGC TGGGCAGCAT TGCAGGTGTC ATGGCGACGC TTGGCTACCC ACTGCCCCAG 
CCTCTGCCAG GGACTGAGCC AGCCTGGGCC CCTGGCCCTG CCCACAGTGA CTTCCTCCAG 
AAGATGGATG ACTTCTGGCT GCTGAAGGAG CTGCAGACCT GGCTATGGCG TTCAGCCAAG 
GACTTCAACC GGCTTAAGAA GAAGATGCAG CCTCCAGCAG CTTCAGTCAC CCTGCACTTG 
GAGGCCCATG GTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders.
Cytokine & growth factor reviews26(5)517-22(2015 Oct)
Sims NA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

DJ-1 enhances cell survival through the binding of Cezanne, a negative regulator of NF-kappaB.
The Journal of biological chemistry286(6)4098-106(2011 Feb)
McNally RS,Davis BK,Clements CM,Accavitti-Loper MA,Mak TW,Ting JP

Ciliary neurotrophic factor inhibits bone formation and plays a sex-specific role in bone growth and remodeling.
Calcified tissue international86(3)261-70(2010 Mar)
McGregor NE,Poulton IJ,Walker EC,Pompolo S,Quinn JM,Martin TJ,Sims NA

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Clcf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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