Pigo cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pigo
Entrez Gene ID	56703
Full Name	phosphatidylinositol glycan anchor biosynthesis, class O
Synonyms	PIG-O
General protein information	Preferred Names phosphatidylinositol glycan anchor biosynthesis, class O Names GPI ethanolamine phosphate transferase 3 phosphatidylinositol-glycan biosynthesis class O protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 A5

mRNA and Protein(s)

mRNA	Protein	Name
XM_030253685.1	XP_030109545.1	GPI ethanolamine phosphate transferase 3 isoform X2
XM_006538113.2	XP_006538176.1	GPI ethanolamine phosphate transferase 3 isoform X1
XM_011250078.2	XP_011248380.1	GPI ethanolamine phosphate transferase 3 isoform X1
NM_020035.3	NP_064419.2	GPI ethanolamine phosphate transferase 3 isoform 1
XM_006538113.3	XP_006538176.1	GPI ethanolamine phosphate transferase 3 isoform X1
XM_011250078.3	XP_011248380.1	GPI ethanolamine phosphate transferase 3 isoform X1
NM_001305387.1	NP_001292316.1	GPI ethanolamine phosphate transferase 3 isoform 3
NM_001305386.1	NP_001292315.1	GPI ethanolamine phosphate transferase 3 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PIGO	XP_001165498.1
Canis lupus familiaris (dog)	PIGO	XP_005626807.1
Rattus norvegicus (Norway rat)	Pigo	XP_233141.7
Gallus gallus (chicken)	PIGO	XP_004937176.1
Drosophila melanogaster (fruit fly)	CG12263	NP_611332.1
Homo sapiens (human)	PIGO	NP_116023.2
Mus musculus (house mouse)	Pigo	NP_064419.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006269	XP_316333.3
Caenorhabditis elegans (roundworm)	C27A12.9	NP_491750.3
Xenopus tropicalis (tropical clawed frog)	pigo	NP_001120383.1
	PIGO	XP_001091501.1
Bos taurus (cattle)	PIGO	XP_002689720.1
Danio rerio (zebrafish)	pigo	XP_692445.3

Gene Ontology
Bibliography

Related articles in PubMed

GPI7 is the second partner of PIG-F and involved in modification of glycosylphosphatidylinositol.
Shishioh N, Hong Y, Ohishi K, Ashida H, Maeda Y, Kinoshita T
The Journal of biological chemistry280(10)9728-34(2005 Mar)

Requirement of PIG-F and PIG-O for transferring phosphoethanolamine to the third mannose in glycosylphosphatidylinositol.
Hong Y, Maeda Y, Watanabe R, Inoue N, Ohishi K, Kinoshita T
The Journal of biological chemistry275(27)20911-9(2000 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pigo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pigo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu88594	XM_030253685.1 Latest version!	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu33064	XM_011250078.3 Latest version!	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu33064	XM_006538113.3 Latest version!	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu11824	NM_020035.3 Latest version!	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu33064	NM_001305386.1 Latest version!	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu69674	NM_001305387.1 Latest version!	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu33064	XM_006538113.2	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu33064	XM_011250078.2	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pigo ( XM_030253685.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109545.1 Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu88594
Clone ID Related Accession (Same CDS sequence)	XM_030253685.1
Accession Version	XM_030253685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GPI ethanolamine phosphate transferase 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATGA ATGGGGACCA TGGAGGGGAC AGTGAGCTGG AGGTGTCAGC TGCACTTTTC 
CTGTACAGCC CCACAGCTCT CTTCCCTAGT GTCCCACCCG AGGAGCCGGA GGTTATTCCT 
CAGGTCAGCC TTGTGCCCAC GCTGGCACTG CTGCTAGGCC TGCCCATCCC GTTTGGGAAC 
ACTGGGGAAG TGATGGCAGA GCTATTCTCA GGTGGCAGTG ACTCCTCCCA TCCTCACTCC 
TCTGCGCTAG CCCAAGTCTC GGCTCTCCAC ATCAATGCCC AGCAGGTGTC CCGGTTTCTT 
CATACCTACT CGGCTGCGAC TCAGGATCTC CAAGTTAAAG AACTTCATCG GCTGCAGACG 
CTCTTCTCCA AGGCCTCTGC GCGTTACCAG CACTTTCTGC GGGATCCCCA AGAGGCTGAG 
GCAGCGCTGA GCACATTGAC TGCTGAGTTC CAGCAGTTCC TGCGGGGAGC TCGGGCCCTG 
TGCATCGAGT CTTGGGCCCG CTTCTCTCTG GTCCGAATGG CAGGGGGCGC TGCTCTTTTG 
GCTGCTGCCT GCCTTCTCTG CCTGCTTGCT TCCCAGTTGG CAGTCGCCCC AGGCTTTCTC 
TTCCGCCCAC TACTGCTAAT ACCTGTAGCC TGGGGCCTAA CTTGGACCAT CCTGTATGCT 
GGAGTCTCGG TAACTACTGG GTCGAAGATA GACTTAGTGG TTCTGGGGGC TGTGGCTGCA 
GCCGGTTCAC TCCTCCCTTT TCTGTGGAAA GCTTGGGTTA GCAGGGGGTC TAAGAGGCCA 
CTGGCTCCCC TACTTCCTGT CCCTAGACCT GTCCTAATAT TACTGCTCAT TCGCTTGGCC 
ACCTTCTTCT CGGATAGCTT TGTTGTTGCT GAGGCCAGGG CCACCCCCTT CCTTCTGGGC 
TCTCTCGTTT TCTTCCTGGT TGCTCAGCTC CACTGGGAGG GTCAGCTACT GCCACCCAAA 
CCACTCACGA TGTCCCGCCT TGGCTCTTCT GCCCCAACAG CGCCCCCACG GCACAGTGGC 
GCGCATGCCC TGTGGCTTGG AATTGGGTTG CTTTTATTTA CAAGGCTCGC TGGGCTTTTT 
CACCGCTGCC CGGAAGAGAC ACCTGCCTGT CGCTCCTCTC CCTGGCTGAG TCCTCTGGCA 
TCTATGGTGG GAGGTCGAGC CAAGAATTTG TGGTACGGAG CTTGCGTGGG GGCACTGGTG 
GCCCTGTTAG TCGTCGTGCG CTTGTGGCTT CGCCGCTATG GTAATCTCAA GAGTCCTGAG 
CCCCCTGTGC TCTTTGTGCG CTGGGGGATG CCTCTCATGG TACTGGGCAC GGCTGCCTAC 
TGGGCACTGG CATCAGGTGC AGAGGAAGCA CCACCACGCC TCCGGGCCCT GGTCGCCGGG 
GCATCAGCTG TGCTGCCTCG GGCTGTGATG GGGCTGGCTG CCTTGGGGCT CGTGCTGCTG 
CTCTGGAGGC CTGTGACAGT GCTGGTGAAA GCTGGGGCTG CTACTTCAAG GACCAGGACT 
ATCCTCACTC CCTTCTCAGG TCCCCCTACT TCTCAGGCTG ACCTCGACTA TGTAGTCCCA 
CAAATCTACC GCCACATGCA GGAGGAGTTC CAGGGCCGGC TCGAGAGGAC TAAAGCTCAG 
GGTCCCATAA CCGTGGCTGC CTATCAGTTG GGAAGCGTGT ACTCGGCTGC TATGGTCACG 
GCCCTCCTCC TCTTGGCCTT CCCACTTATG CTGTTGCATG TAGAGCGGGT CAGCCTGGTG 
TTCCTGCTCC TGTTTCTGCA GAGCTTCCTT CTCCTGCATC TGCTGGCCGC TGGGACACCA 
GTCGCCACCC CTGGCCCTTT TACTGTGCTG TGGCAGGCAG TCTCAGCTTG GGTCCTTCTG 
GCTACCCAGA CCTTCTACTC CACAGGCCAC CAGCCTGTCT TTTCAGCCAT CCATTGGCAC 
GCAGCCTTTG TGGGCTTCCC AGACGGCCAT GGCTCTTCTA CCTGGTTGCC TGCCTTACTA 
GTGGGAGCTA ACACCTTTGC CTCCCACCTC CTCTTCGCAG TAGGCTGCCC ACTGCTCCTG 
CTCTGGCCCT TCCTGTGTGA GCGTCAAGGG CCAAAGAGAA GGCAGCCCCT CCCGGGAAGT 
GAGTCTGAAG CCAGAGTCCG GCCTGAGGAA GAGGAGGAGC TGCAGGAGCC ACTGATGGAG 
ATGCGCCTCC GGGATGCTCC GAATCACTTC AATGCAGCCC TGTTGCAGCT GGGCCTCAAG 
TACCTCTTTA TCCTTGGAGC TCAGATTCTA GCTTGTGCCT TGGCAGCTTC CATCCTCCGC 
AGGCACCTCA TGGTCTGGAA GGTGTTTGCT CCCAAGTTCA TTTTTGAAGC CGTAGGTTTC 
ATCGTGAGCA GTGTGGGACT TCTCCTGGGC ATAGCATTGG TGATGCGAGT GGATGTCGCT 
GTGAGTTCCT GGTTCAAAAA ACTAGTTCTG GCCCAGCAGA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109545.1
CDS	1965..4409
Translation	MTMNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLASQLAVAPGFLFRPLLLIPVAWGLTWTILYAGVSVTTGSKIDLVVLGAVAAAGSLLPFLWKAWVSRGSKRPLAPLLPVPRPVLILLLIRLATFFSDSFVVAEARATPFLLGSLVFFLVAQLHWEGQLLPPKPLTMSRLGSSAPTAPPRHSGAHALWLGIGLLLFTRLAGLFHRCPEETPACRSSPWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPEPPVLFVRWGMPLMVLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWRPVTVLVKAGAATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSVYSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPFTVLWQAVSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEPLMEMRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR

Target ORF information:

RefSeq Version	XM_030253685.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253685.1

ORF Insert Sequence:

ATGACCATGA ATGGGGACCA TGGAGGGGAC AGTGAGCTGG AGGTGTCAGC TGCACTTTTC 
CTGTACAGCC CCACAGCTCT CTTCCCTAGT GTCCCACCCG AGGAGCCGGA GGTTATTCCT 
CAGGTCAGCC TTGTGCCCAC GCTGGCACTG CTGCTAGGCC TGCCCATCCC GTTTGGGAAC 
ACTGGGGAAG TGATGGCAGA GCTATTCTCA GGTGGCAGTG ACTCCTCCCA TCCTCACTCC 
TCTGCGCTAG CCCAAGTCTC GGCTCTCCAC ATCAATGCCC AGCAGGTGTC CCGGTTTCTT 
CATACCTACT CGGCTGCGAC TCAGGATCTC CAAGTTAAAG AACTTCATCG GCTGCAGACG 
CTCTTCTCCA AGGCCTCTGC GCGTTACCAG CACTTTCTGC GGGATCCCCA AGAGGCTGAG 
GCAGCGCTGA GCACATTGAC TGCTGAGTTC CAGCAGTTCC TGCGGGGAGC TCGGGCCCTG 
TGCATCGAGT CTTGGGCCCG CTTCTCTCTG GTCCGAATGG CAGGGGGCGC TGCTCTTTTG 
GCTGCTGCCT GCCTTCTCTG CCTGCTTGCT TCCCAGTTGG CAGTCGCCCC AGGCTTTCTC 
TTCCGCCCAC TACTGCTAAT ACCTGTAGCC TGGGGCCTAA CTTGGACCAT CCTGTATGCT 
GGAGTCTCGG TAACTACTGG GTCGAAGATA GACTTAGTGG TTCTGGGGGC TGTGGCTGCA 
GCCGGTTCAC TCCTCCCTTT TCTGTGGAAA GCTTGGGTTA GCAGGGGGTC TAAGAGGCCA 
CTGGCTCCCC TACTTCCTGT CCCTAGACCT GTCCTAATAT TACTGCTCAT TCGCTTGGCC 
ACCTTCTTCT CGGATAGCTT TGTTGTTGCT GAGGCCAGGG CCACCCCCTT CCTTCTGGGC 
TCTCTCGTTT TCTTCCTGGT TGCTCAGCTC CACTGGGAGG GTCAGCTACT GCCACCCAAA 
CCACTCACGA TGTCCCGCCT TGGCTCTTCT GCCCCAACAG CGCCCCCACG GCACAGTGGC 
GCGCATGCCC TGTGGCTTGG AATTGGGTTG CTTTTATTTA CAAGGCTCGC TGGGCTTTTT 
CACCGCTGCC CGGAAGAGAC ACCTGCCTGT CGCTCCTCTC CCTGGCTGAG TCCTCTGGCA 
TCTATGGTGG GAGGTCGAGC CAAGAATTTG TGGTACGGAG CTTGCGTGGG GGCACTGGTG 
GCCCTGTTAG TCGTCGTGCG CTTGTGGCTT CGCCGCTATG GTAATCTCAA GAGTCCTGAG 
CCCCCTGTGC TCTTTGTGCG CTGGGGGATG CCTCTCATGG TACTGGGCAC GGCTGCCTAC 
TGGGCACTGG CATCAGGTGC AGAGGAAGCA CCACCACGCC TCCGGGCCCT GGTCGCCGGG 
GCATCAGCTG TGCTGCCTCG GGCTGTGATG GGGCTGGCTG CCTTGGGGCT CGTGCTGCTG 
CTCTGGAGGC CTGTGACAGT GCTGGTGAAA GCTGGGGCTG CTACTTCAAG GACCAGGACT 
ATCCTCACTC CCTTCTCAGG TCCCCCTACT TCTCAGGCTG ACCTCGACTA TGTAGTCCCA 
CAAATCTACC GCCACATGCA GGAGGAGTTC CAGGGCCGGC TCGAGAGGAC TAAAGCTCAG 
GGTCCCATAA CCGTGGCTGC CTATCAGTTG GGAAGCGTGT ACTCGGCTGC TATGGTCACG 
GCCCTCCTCC TCTTGGCCTT CCCACTTATG CTGTTGCATG TAGAGCGGGT CAGCCTGGTG 
TTCCTGCTCC TGTTTCTGCA GAGCTTCCTT CTCCTGCATC TGCTGGCCGC TGGGACACCA 
GTCGCCACCC CTGGCCCTTT TACTGTGCTG TGGCAGGCAG TCTCAGCTTG GGTCCTTCTG 
GCTACCCAGA CCTTCTACTC CACAGGCCAC CAGCCTGTCT TTTCAGCCAT CCATTGGCAC 
GCAGCCTTTG TGGGCTTCCC AGACGGCCAT GGCTCTTCTA CCTGGTTGCC TGCCTTACTA 
GTGGGAGCTA ACACCTTTGC CTCCCACCTC CTCTTCGCAG TAGGCTGCCC ACTGCTCCTG 
CTCTGGCCCT TCCTGTGTGA GCGTCAAGGG CCAAAGAGAA GGCAGCCCCT CCCGGGAAGT 
GAGTCTGAAG CCAGAGTCCG GCCTGAGGAA GAGGAGGAGC TGCAGGAGCC ACTGATGGAG 
ATGCGCCTCC GGGATGCTCC GAATCACTTC AATGCAGCCC TGTTGCAGCT GGGCCTCAAG 
TACCTCTTTA TCCTTGGAGC TCAGATTCTA GCTTGTGCCT TGGCAGCTTC CATCCTCCGC 
AGGCACCTCA TGGTCTGGAA GGTGTTTGCT CCCAAGTTCA TTTTTGAAGC CGTAGGTTTC 
ATCGTGAGCA GTGTGGGACT TCTCCTGGGC ATAGCATTGG TGATGCGAGT GGATGTCGCT 
GTGAGTTCCT GGTTCAAAAA ACTAGTTCTG GCCCAGCAGA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pigo ( XM_006538113.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538176.1 Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33064
Clone ID Related Accession (Same CDS sequence)	XM_006538113.2 , XM_011250078.2 , XM_006538113.3 , XM_011250078.3 , NM_001305386.1
Accession Version	XM_006538113.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GPI ethanolamine phosphate transferase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538113.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAAGG TGTCTGTGCT CCTCTTCTTA GCCTGGGTCT GTTTTCTTTT CTACGCTGGC 
ATTGCACTCT TCACCAGTGG CTTCCTTCTC ACTCGTCTGG AGCTCACCAA TCAAAGCAGC 
TGCCAAGAGC TCCCAGGCCC TGGTCCCTTG CCGTGGGGAA GTCATGGAAA GCCTGGGGCT 
TGCTGGATGC CTTCCCGCTT CTCCAGGGTG GTTTTGGTAC TGATAGACGC TCTGCGGTTT 
GACTTTGCCC AGCCTCAGCG CTCGCATGTG CCTGGGGAAC CTCCTGTCTC CGTGCCTTTT 
CTGGGAAAAT TAGGTTCCTT GCAGAGGATC CTAGAGAGTC AACCCCACCA TGGCCGGCTG 
TACCGATCTC AGGTGGACCC CCCCACGACC ACCATGCAGC GTCTAAAGGC TCTCACCACT 
GGCTCGCTGC CGACTTTCAT TGATGCTGGC AGTAACTTTG CCAGCCATGC TATAGTGGAA 
GATAATGTCA TCCAACAGCT CAACAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT 
ACCTGGAGAG ACCTCTTCCC CGGAGCCTTT TCTCAAGCTT TCTTCTTCTC ATCCTTCAAT 
GTCAGAGACC TGCACACAGT GGATAACGGC ATCTTGGAAC ACCTCTATCC TACCCTGGAC 
GGCGGTTCGT GGGATGTGCT GATCGCTCAC TTCCTGGGTG TGGATCACTG TGGTCACAAG 
CACGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATACAG 
GGACTCATAG AACGCCTGGA GAATGACACA CTGCTGGTGG TAGCCGGTGA CCATGGGATG 
ACCATGAATG GGGACCATGG AGGGGACAGT GAGCTGGAGG TGTCAGCTGC ACTTTTCCTG 
TACAGCCCCA CAGCTCTCTT CCCTAGTGTC CCACCCGAGG AGCCGGAGGT TATTCCTCAG 
GTCAGCCTTG TGCCCACGCT GGCACTGCTG CTAGGCCTGC CCATCCCGTT TGGGAACACT 
GGGGAAGTGA TGGCAGAGCT ATTCTCAGGT GGCAGTGACT CCTCCCATCC TCACTCCTCT 
GCGCTAGCCC AAGTCTCGGC TCTCCACATC AATGCCCAGC AGGTGTCCCG GTTTCTTCAT 
ACCTACTCGG CTGCGACTCA GGATCTCCAA GTTAAAGAAC TTCATCGGCT GCAGACGCTC 
TTCTCCAAGG CCTCTGCGCG TTACCAGCAC TTTCTGCGGG ATCCCCAAGA GGCTGAGGCA 
GCGCTGAGCA CATTGACTGC TGAGTTCCAG CAGTTCCTGC GGGGAGCTCG GGCCCTGTGC 
ATCGAGTCTT GGGCCCGCTT CTCTCTGGTC CGAATGGCAG GGGGCGCTGC TCTTTTGGCT 
GCTGCCTGCC TTCTCTGCCT GCTTGCTTCC CAGTTGGCAG TCGCCCCAGG CTTTCTCTTC 
CGCCCACTAC TGCTAATACC TGTAGCCTGG GGCCTAACTT GGACCATCCT GTATGCTGGA 
GTCTCGGTAA CTACTGGGTC GAAGATAGAC TTAGTGGTTC TGGGGGCTGT GGCTGCAGCC 
GGTTCACTCC TCCCTTTTCT GTGGAAAGCT TGGGTTAGCA GGGGGTCTAA GAGGCCACTG 
GCTCCCCTAC TTCCTGTCCC TAGACCTGTC CTAATATTAC TGCTCATTCG CTTGGCCACC 
TTCTTCTCGG ATAGCTTTGT TGTTGCTGAG GCCAGGGCCA CCCCCTTCCT TCTGGGCTCT 
CTCGTTTTCT TCCTGGTTGC TCAGCTCCAC TGGGAGGGTC AGCTACTGCC ACCCAAACCA 
CTCACGATGT CCCGCCTTGG CTCTTCTGCC CCAACAGCGC CCCCACGGCA CAGTGGCGCG 
CATGCCCTGT GGCTTGGAAT TGGGTTGCTT TTATTTACAA GGCTCGCTGG GCTTTTTCAC 
CGCTGCCCGG AAGAGACACC TGCCTGTCGC TCCTCTCCCT GGCTGAGTCC TCTGGCATCT 
ATGGTGGGAG GTCGAGCCAA GAATTTGTGG TACGGAGCTT GCGTGGGGGC ACTGGTGGCC 
CTGTTAGTCG TCGTGCGCTT GTGGCTTCGC CGCTATGGTA ATCTCAAGAG TCCTGAGCCC 
CCTGTGCTCT TTGTGCGCTG GGGGATGCCT CTCATGGTAC TGGGCACGGC TGCCTACTGG 
GCACTGGCAT CAGGTGCAGA GGAAGCACCA CCACGCCTCC GGGCCCTGGT CGCCGGGGCA 
TCAGCTGTGC TGCCTCGGGC TGTGATGGGG CTGGCTGCCT TGGGGCTCGT GCTGCTGCTC 
TGGAGGCCTG TGACAGTGCT GGTGAAAGCT GGGGCTGCTA CTTCAAGGAC CAGGACTATC 
CTCACTCCCT TCTCAGGTCC CCCTACTTCT CAGGCTGACC TCGACTATGT AGTCCCACAA 
ATCTACCGCC ACATGCAGGA GGAGTTCCAG GGCCGGCTCG AGAGGACTAA AGCTCAGGGT 
CCCATAACCG TGGCTGCCTA TCAGTTGGGA AGCGTGTACT CGGCTGCTAT GGTCACGGCC 
CTCCTCCTCT TGGCCTTCCC ACTTATGCTG TTGCATGTAG AGCGGGTCAG CCTGGTGTTC 
CTGCTCCTGT TTCTGCAGAG CTTCCTTCTC CTGCATCTGC TGGCCGCTGG GACACCAGTC 
GCCACCCCTG GCCCTTTTAC TGTGCTGTGG CAGGCAGTCT CAGCTTGGGT CCTTCTGGCT 
ACCCAGACCT TCTACTCCAC AGGCCACCAG CCTGTCTTTT CAGCCATCCA TTGGCACGCA 
GCCTTTGTGG GCTTCCCAGA CGGCCATGGC TCTTCTACCT GGTTGCCTGC CTTACTAGTG 
GGAGCTAACA CCTTTGCCTC CCACCTCCTC TTCGCAGTAG GCTGCCCACT GCTCCTGCTC 
TGGCCCTTCC TGTGTGAGCG TCAAGGGCCA AAGAGAAGGC AGCCCCTCCC GGGAAGTGAG 
TCTGAAGCCA GAGTCCGGCC TGAGGAAGAG GAGGAGCTGC AGGAGCCACT GATGGAGATG 
CGCCTCCGGG ATGCTCCGAA TCACTTCAAT GCAGCCCTGT TGCAGCTGGG CCTCAAGTAC 
CTCTTTATCC TTGGAGCTCA GATTCTAGCT TGTGCCTTGG CAGCTTCCAT CCTCCGCAGG 
CACCTCATGG TCTGGAAGGT GTTTGCTCCC AAGTTCATTT TTGAAGCCGT AGGTTTCATC 
GTGAGCAGTG TGGGACTTCT CCTGGGCATA GCATTGGTGA TGCGAGTGGA TGTCGCTGTG 
AGTTCCTGGT TCAAAAAACT AGTTCTGGCC CAGCAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538176.1
CDS	1136..4417
Translation	MRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLASQLAVAPGFLFRPLLLIPVAWGLTWTILYAGVSVTTGSKIDLVVLGAVAAAGSLLPFLWKAWVSRGSKRPLAPLLPVPRPVLILLLIRLATFFSDSFVVAEARATPFLLGSLVFFLVAQLHWEGQLLPPKPLTMSRLGSSAPTAPPRHSGAHALWLGIGLLLFTRLAGLFHRCPEETPACRSSPWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPEPPVLFVRWGMPLMVLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWRPVTVLVKAGAATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSVYSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPFTVLWQAVSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEPLMEMRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR

Target ORF information:

RefSeq Version	XM_006538113.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538113.3

ORF Insert Sequence:

ATGCGGAAGG TGTCTGTGCT CCTCTTCTTA GCCTGGGTCT GTTTTCTTTT CTACGCTGGC 
ATTGCACTCT TCACCAGTGG CTTCCTTCTC ACTCGTCTGG AGCTCACCAA TCAAAGCAGC 
TGCCAAGAGC TCCCAGGCCC TGGTCCCTTG CCGTGGGGAA GTCATGGAAA GCCTGGGGCT 
TGCTGGATGC CTTCCCGCTT CTCCAGGGTG GTTTTGGTAC TGATAGACGC TCTGCGGTTT 
GACTTTGCCC AGCCTCAGCG CTCGCATGTG CCTGGGGAAC CTCCTGTCTC CGTGCCTTTT 
CTGGGAAAAT TAGGTTCCTT GCAGAGGATC CTAGAGAGTC AACCCCACCA TGGCCGGCTG 
TACCGATCTC AGGTGGACCC CCCCACGACC ACCATGCAGC GTCTAAAGGC TCTCACCACT 
GGCTCGCTGC CGACTTTCAT TGATGCTGGC AGTAACTTTG CCAGCCATGC TATAGTGGAA 
GATAATGTCA TCCAACAGCT CAACAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT 
ACCTGGAGAG ACCTCTTCCC CGGAGCCTTT TCTCAAGCTT TCTTCTTCTC ATCCTTCAAT 
GTCAGAGACC TGCACACAGT GGATAACGGC ATCTTGGAAC ACCTCTATCC TACCCTGGAC 
GGCGGTTCGT GGGATGTGCT GATCGCTCAC TTCCTGGGTG TGGATCACTG TGGTCACAAG 
CACGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATACAG 
GGACTCATAG AACGCCTGGA GAATGACACA CTGCTGGTGG TAGCCGGTGA CCATGGGATG 
ACCATGAATG GGGACCATGG AGGGGACAGT GAGCTGGAGG TGTCAGCTGC ACTTTTCCTG 
TACAGCCCCA CAGCTCTCTT CCCTAGTGTC CCACCCGAGG AGCCGGAGGT TATTCCTCAG 
GTCAGCCTTG TGCCCACGCT GGCACTGCTG CTAGGCCTGC CCATCCCGTT TGGGAACACT 
GGGGAAGTGA TGGCAGAGCT ATTCTCAGGT GGCAGTGACT CCTCCCATCC TCACTCCTCT 
GCGCTAGCCC AAGTCTCGGC TCTCCACATC AATGCCCAGC AGGTGTCCCG GTTTCTTCAT 
ACCTACTCGG CTGCGACTCA GGATCTCCAA GTTAAAGAAC TTCATCGGCT GCAGACGCTC 
TTCTCCAAGG CCTCTGCGCG TTACCAGCAC TTTCTGCGGG ATCCCCAAGA GGCTGAGGCA 
GCGCTGAGCA CATTGACTGC TGAGTTCCAG CAGTTCCTGC GGGGAGCTCG GGCCCTGTGC 
ATCGAGTCTT GGGCCCGCTT CTCTCTGGTC CGAATGGCAG GGGGCGCTGC TCTTTTGGCT 
GCTGCCTGCC TTCTCTGCCT GCTTGCTTCC CAGTTGGCAG TCGCCCCAGG CTTTCTCTTC 
CGCCCACTAC TGCTAATACC TGTAGCCTGG GGCCTAACTT GGACCATCCT GTATGCTGGA 
GTCTCGGTAA CTACTGGGTC GAAGATAGAC TTAGTGGTTC TGGGGGCTGT GGCTGCAGCC 
GGTTCACTCC TCCCTTTTCT GTGGAAAGCT TGGGTTAGCA GGGGGTCTAA GAGGCCACTG 
GCTCCCCTAC TTCCTGTCCC TAGACCTGTC CTAATATTAC TGCTCATTCG CTTGGCCACC 
TTCTTCTCGG ATAGCTTTGT TGTTGCTGAG GCCAGGGCCA CCCCCTTCCT TCTGGGCTCT 
CTCGTTTTCT TCCTGGTTGC TCAGCTCCAC TGGGAGGGTC AGCTACTGCC ACCCAAACCA 
CTCACGATGT CCCGCCTTGG CTCTTCTGCC CCAACAGCGC CCCCACGGCA CAGTGGCGCG 
CATGCCCTGT GGCTTGGAAT TGGGTTGCTT TTATTTACAA GGCTCGCTGG GCTTTTTCAC 
CGCTGCCCGG AAGAGACACC TGCCTGTCGC TCCTCTCCCT GGCTGAGTCC TCTGGCATCT 
ATGGTGGGAG GTCGAGCCAA GAATTTGTGG TACGGAGCTT GCGTGGGGGC ACTGGTGGCC 
CTGTTAGTCG TCGTGCGCTT GTGGCTTCGC CGCTATGGTA ATCTCAAGAG TCCTGAGCCC 
CCTGTGCTCT TTGTGCGCTG GGGGATGCCT CTCATGGTAC TGGGCACGGC TGCCTACTGG 
GCACTGGCAT CAGGTGCAGA GGAAGCACCA CCACGCCTCC GGGCCCTGGT CGCCGGGGCA 
TCAGCTGTGC TGCCTCGGGC TGTGATGGGG CTGGCTGCCT TGGGGCTCGT GCTGCTGCTC 
TGGAGGCCTG TGACAGTGCT GGTGAAAGCT GGGGCTGCTA CTTCAAGGAC CAGGACTATC 
CTCACTCCCT TCTCAGGTCC CCCTACTTCT CAGGCTGACC TCGACTATGT AGTCCCACAA 
ATCTACCGCC ACATGCAGGA GGAGTTCCAG GGCCGGCTCG AGAGGACTAA AGCTCAGGGT 
CCCATAACCG TGGCTGCCTA TCAGTTGGGA AGCGTGTACT CGGCTGCTAT GGTCACGGCC 
CTCCTCCTCT TGGCCTTCCC ACTTATGCTG TTGCATGTAG AGCGGGTCAG CCTGGTGTTC 
CTGCTCCTGT TTCTGCAGAG CTTCCTTCTC CTGCATCTGC TGGCCGCTGG GACACCAGTC 
GCCACCCCTG GCCCTTTTAC TGTGCTGTGG CAGGCAGTCT CAGCTTGGGT CCTTCTGGCT 
ACCCAGACCT TCTACTCCAC AGGCCACCAG CCTGTCTTTT CAGCCATCCA TTGGCACGCA 
GCCTTTGTGG GCTTCCCAGA CGGCCATGGC TCTTCTACCT GGTTGCCTGC CTTACTAGTG 
GGAGCTAACA CCTTTGCCTC CCACCTCCTC TTCGCAGTAG GCTGCCCACT GCTCCTGCTC 
TGGCCCTTCC TGTGTGAGCG TCAAGGGCCA AAGAGAAGGC AGCCCCTCCC GGGAAGTGAG 
TCTGAAGCCA GAGTCCGGCC TGAGGAAGAG GAGGAGCTGC AGGAGCCACT GATGGAGATG 
CGCCTCCGGG ATGCTCCGAA TCACTTCAAT GCAGCCCTGT TGCAGCTGGG CCTCAAGTAC 
CTCTTTATCC TTGGAGCTCA GATTCTAGCT TGTGCCTTGG CAGCTTCCAT CCTCCGCAGG 
CACCTCATGG TCTGGAAGGT GTTTGCTCCC AAGTTCATTT TTGAAGCCGT AGGTTTCATC 
GTGAGCAGTG TGGGACTTCT CCTGGGCATA GCATTGGTGA TGCGAGTGGA TGTCGCTGTG 
AGTTCCTGGT TCAAAAAACT AGTTCTGGCC CAGCAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pigo ( XM_011250078.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248380.1 Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X2, mRNA.

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CloneID	OMu33064
Clone ID Related Accession (Same CDS sequence)	XM_006538113.2 , XM_011250078.2 , XM_006538113.3 , XM_011250078.3 , NM_001305386.1
Accession Version	XM_011250078.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GPI ethanolamine phosphate transferase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250078.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAAGG TGTCTGTGCT CCTCTTCTTA GCCTGGGTCT GTTTTCTTTT CTACGCTGGC 
ATTGCACTCT TCACCAGTGG CTTCCTTCTC ACTCGTCTGG AGCTCACCAA TCAAAGCAGC 
TGCCAAGAGC TCCCAGGCCC TGGTCCCTTG CCGTGGGGAA GTCATGGAAA GCCTGGGGCT 
TGCTGGATGC CTTCCCGCTT CTCCAGGGTG GTTTTGGTAC TGATAGACGC TCTGCGGTTT 
GACTTTGCCC AGCCTCAGCG CTCGCATGTG CCTGGGGAAC CTCCTGTCTC CGTGCCTTTT 
CTGGGAAAAT TAGGTTCCTT GCAGAGGATC CTAGAGAGTC AACCCCACCA TGGCCGGCTG 
TACCGATCTC AGGTGGACCC CCCCACGACC ACCATGCAGC GTCTAAAGGC TCTCACCACT 
GGCTCGCTGC CGACTTTCAT TGATGCTGGC AGTAACTTTG CCAGCCATGC TATAGTGGAA 
GATAATGTCA TCCAACAGCT CAACAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT 
ACCTGGAGAG ACCTCTTCCC CGGAGCCTTT TCTCAAGCTT TCTTCTTCTC ATCCTTCAAT 
GTCAGAGACC TGCACACAGT GGATAACGGC ATCTTGGAAC ACCTCTATCC TACCCTGGAC 
GGCGGTTCGT GGGATGTGCT GATCGCTCAC TTCCTGGGTG TGGATCACTG TGGTCACAAG 
CACGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATACAG 
GGACTCATAG AACGCCTGGA GAATGACACA CTGCTGGTGG TAGCCGGTGA CCATGGGATG 
ACCATGAATG GGGACCATGG AGGGGACAGT GAGCTGGAGG TGTCAGCTGC ACTTTTCCTG 
TACAGCCCCA CAGCTCTCTT CCCTAGTGTC CCACCCGAGG AGCCGGAGGT TATTCCTCAG 
GTCAGCCTTG TGCCCACGCT GGCACTGCTG CTAGGCCTGC CCATCCCGTT TGGGAACACT 
GGGGAAGTGA TGGCAGAGCT ATTCTCAGGT GGCAGTGACT CCTCCCATCC TCACTCCTCT 
GCGCTAGCCC AAGTCTCGGC TCTCCACATC AATGCCCAGC AGGTGTCCCG GTTTCTTCAT 
ACCTACTCGG CTGCGACTCA GGATCTCCAA GTTAAAGAAC TTCATCGGCT GCAGACGCTC 
TTCTCCAAGG CCTCTGCGCG TTACCAGCAC TTTCTGCGGG ATCCCCAAGA GGCTGAGGCA 
GCGCTGAGCA CATTGACTGC TGAGTTCCAG CAGTTCCTGC GGGGAGCTCG GGCCCTGTGC 
ATCGAGTCTT GGGCCCGCTT CTCTCTGGTC CGAATGGCAG GGGGCGCTGC TCTTTTGGCT 
GCTGCCTGCC TTCTCTGCCT GCTTGCTTCC CAGTTGGCAG TCGCCCCAGG CTTTCTCTTC 
CGCCCACTAC TGCTAATACC TGTAGCCTGG GGCCTAACTT GGACCATCCT GTATGCTGGA 
GTCTCGGTAA CTACTGGGTC GAAGATAGAC TTAGTGGTTC TGGGGGCTGT GGCTGCAGCC 
GGTTCACTCC TCCCTTTTCT GTGGAAAGCT TGGGTTAGCA GGGGGTCTAA GAGGCCACTG 
GCTCCCCTAC TTCCTGTCCC TAGACCTGTC CTAATATTAC TGCTCATTCG CTTGGCCACC 
TTCTTCTCGG ATAGCTTTGT TGTTGCTGAG GCCAGGGCCA CCCCCTTCCT TCTGGGCTCT 
CTCGTTTTCT TCCTGGTTGC TCAGCTCCAC TGGGAGGGTC AGCTACTGCC ACCCAAACCA 
CTCACGATGT CCCGCCTTGG CTCTTCTGCC CCAACAGCGC CCCCACGGCA CAGTGGCGCG 
CATGCCCTGT GGCTTGGAAT TGGGTTGCTT TTATTTACAA GGCTCGCTGG GCTTTTTCAC 
CGCTGCCCGG AAGAGACACC TGCCTGTCGC TCCTCTCCCT GGCTGAGTCC TCTGGCATCT 
ATGGTGGGAG GTCGAGCCAA GAATTTGTGG TACGGAGCTT GCGTGGGGGC ACTGGTGGCC 
CTGTTAGTCG TCGTGCGCTT GTGGCTTCGC CGCTATGGTA ATCTCAAGAG TCCTGAGCCC 
CCTGTGCTCT TTGTGCGCTG GGGGATGCCT CTCATGGTAC TGGGCACGGC TGCCTACTGG 
GCACTGGCAT CAGGTGCAGA GGAAGCACCA CCACGCCTCC GGGCCCTGGT CGCCGGGGCA 
TCAGCTGTGC TGCCTCGGGC TGTGATGGGG CTGGCTGCCT TGGGGCTCGT GCTGCTGCTC 
TGGAGGCCTG TGACAGTGCT GGTGAAAGCT GGGGCTGCTA CTTCAAGGAC CAGGACTATC 
CTCACTCCCT TCTCAGGTCC CCCTACTTCT CAGGCTGACC TCGACTATGT AGTCCCACAA 
ATCTACCGCC ACATGCAGGA GGAGTTCCAG GGCCGGCTCG AGAGGACTAA AGCTCAGGGT 
CCCATAACCG TGGCTGCCTA TCAGTTGGGA AGCGTGTACT CGGCTGCTAT GGTCACGGCC 
CTCCTCCTCT TGGCCTTCCC ACTTATGCTG TTGCATGTAG AGCGGGTCAG CCTGGTGTTC 
CTGCTCCTGT TTCTGCAGAG CTTCCTTCTC CTGCATCTGC TGGCCGCTGG GACACCAGTC 
GCCACCCCTG GCCCTTTTAC TGTGCTGTGG CAGGCAGTCT CAGCTTGGGT CCTTCTGGCT 
ACCCAGACCT TCTACTCCAC AGGCCACCAG CCTGTCTTTT CAGCCATCCA TTGGCACGCA 
GCCTTTGTGG GCTTCCCAGA CGGCCATGGC TCTTCTACCT GGTTGCCTGC CTTACTAGTG 
GGAGCTAACA CCTTTGCCTC CCACCTCCTC TTCGCAGTAG GCTGCCCACT GCTCCTGCTC 
TGGCCCTTCC TGTGTGAGCG TCAAGGGCCA AAGAGAAGGC AGCCCCTCCC GGGAAGTGAG 
TCTGAAGCCA GAGTCCGGCC TGAGGAAGAG GAGGAGCTGC AGGAGCCACT GATGGAGATG 
CGCCTCCGGG ATGCTCCGAA TCACTTCAAT GCAGCCCTGT TGCAGCTGGG CCTCAAGTAC 
CTCTTTATCC TTGGAGCTCA GATTCTAGCT TGTGCCTTGG CAGCTTCCAT CCTCCGCAGG 
CACCTCATGG TCTGGAAGGT GTTTGCTCCC AAGTTCATTT TTGAAGCCGT AGGTTTCATC 
GTGAGCAGTG TGGGACTTCT CCTGGGCATA GCATTGGTGA TGCGAGTGGA TGTCGCTGTG 
AGTTCCTGGT TCAAAAAACT AGTTCTGGCC CAGCAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248380.1
CDS	830..4111
Translation	MRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLASQLAVAPGFLFRPLLLIPVAWGLTWTILYAGVSVTTGSKIDLVVLGAVAAAGSLLPFLWKAWVSRGSKRPLAPLLPVPRPVLILLLIRLATFFSDSFVVAEARATPFLLGSLVFFLVAQLHWEGQLLPPKPLTMSRLGSSAPTAPPRHSGAHALWLGIGLLLFTRLAGLFHRCPEETPACRSSPWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPEPPVLFVRWGMPLMVLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWRPVTVLVKAGAATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSVYSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPFTVLWQAVSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEPLMEMRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR

Target ORF information:

RefSeq Version	XM_011250078.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250078.3

ORF Insert Sequence:

ATGCGGAAGG TGTCTGTGCT CCTCTTCTTA GCCTGGGTCT GTTTTCTTTT CTACGCTGGC 
ATTGCACTCT TCACCAGTGG CTTCCTTCTC ACTCGTCTGG AGCTCACCAA TCAAAGCAGC 
TGCCAAGAGC TCCCAGGCCC TGGTCCCTTG CCGTGGGGAA GTCATGGAAA GCCTGGGGCT 
TGCTGGATGC CTTCCCGCTT CTCCAGGGTG GTTTTGGTAC TGATAGACGC TCTGCGGTTT 
GACTTTGCCC AGCCTCAGCG CTCGCATGTG CCTGGGGAAC CTCCTGTCTC CGTGCCTTTT 
CTGGGAAAAT TAGGTTCCTT GCAGAGGATC CTAGAGAGTC AACCCCACCA TGGCCGGCTG 
TACCGATCTC AGGTGGACCC CCCCACGACC ACCATGCAGC GTCTAAAGGC TCTCACCACT 
GGCTCGCTGC CGACTTTCAT TGATGCTGGC AGTAACTTTG CCAGCCATGC TATAGTGGAA 
GATAATGTCA TCCAACAGCT CAACAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT 
ACCTGGAGAG ACCTCTTCCC CGGAGCCTTT TCTCAAGCTT TCTTCTTCTC ATCCTTCAAT 
GTCAGAGACC TGCACACAGT GGATAACGGC ATCTTGGAAC ACCTCTATCC TACCCTGGAC 
GGCGGTTCGT GGGATGTGCT GATCGCTCAC TTCCTGGGTG TGGATCACTG TGGTCACAAG 
CACGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATACAG 
GGACTCATAG AACGCCTGGA GAATGACACA CTGCTGGTGG TAGCCGGTGA CCATGGGATG 
ACCATGAATG GGGACCATGG AGGGGACAGT GAGCTGGAGG TGTCAGCTGC ACTTTTCCTG 
TACAGCCCCA CAGCTCTCTT CCCTAGTGTC CCACCCGAGG AGCCGGAGGT TATTCCTCAG 
GTCAGCCTTG TGCCCACGCT GGCACTGCTG CTAGGCCTGC CCATCCCGTT TGGGAACACT 
GGGGAAGTGA TGGCAGAGCT ATTCTCAGGT GGCAGTGACT CCTCCCATCC TCACTCCTCT 
GCGCTAGCCC AAGTCTCGGC TCTCCACATC AATGCCCAGC AGGTGTCCCG GTTTCTTCAT 
ACCTACTCGG CTGCGACTCA GGATCTCCAA GTTAAAGAAC TTCATCGGCT GCAGACGCTC 
TTCTCCAAGG CCTCTGCGCG TTACCAGCAC TTTCTGCGGG ATCCCCAAGA GGCTGAGGCA 
GCGCTGAGCA CATTGACTGC TGAGTTCCAG CAGTTCCTGC GGGGAGCTCG GGCCCTGTGC 
ATCGAGTCTT GGGCCCGCTT CTCTCTGGTC CGAATGGCAG GGGGCGCTGC TCTTTTGGCT 
GCTGCCTGCC TTCTCTGCCT GCTTGCTTCC CAGTTGGCAG TCGCCCCAGG CTTTCTCTTC 
CGCCCACTAC TGCTAATACC TGTAGCCTGG GGCCTAACTT GGACCATCCT GTATGCTGGA 
GTCTCGGTAA CTACTGGGTC GAAGATAGAC TTAGTGGTTC TGGGGGCTGT GGCTGCAGCC 
GGTTCACTCC TCCCTTTTCT GTGGAAAGCT TGGGTTAGCA GGGGGTCTAA GAGGCCACTG 
GCTCCCCTAC TTCCTGTCCC TAGACCTGTC CTAATATTAC TGCTCATTCG CTTGGCCACC 
TTCTTCTCGG ATAGCTTTGT TGTTGCTGAG GCCAGGGCCA CCCCCTTCCT TCTGGGCTCT 
CTCGTTTTCT TCCTGGTTGC TCAGCTCCAC TGGGAGGGTC AGCTACTGCC ACCCAAACCA 
CTCACGATGT CCCGCCTTGG CTCTTCTGCC CCAACAGCGC CCCCACGGCA CAGTGGCGCG 
CATGCCCTGT GGCTTGGAAT TGGGTTGCTT TTATTTACAA GGCTCGCTGG GCTTTTTCAC 
CGCTGCCCGG AAGAGACACC TGCCTGTCGC TCCTCTCCCT GGCTGAGTCC TCTGGCATCT 
ATGGTGGGAG GTCGAGCCAA GAATTTGTGG TACGGAGCTT GCGTGGGGGC ACTGGTGGCC 
CTGTTAGTCG TCGTGCGCTT GTGGCTTCGC CGCTATGGTA ATCTCAAGAG TCCTGAGCCC 
CCTGTGCTCT TTGTGCGCTG GGGGATGCCT CTCATGGTAC TGGGCACGGC TGCCTACTGG 
GCACTGGCAT CAGGTGCAGA GGAAGCACCA CCACGCCTCC GGGCCCTGGT CGCCGGGGCA 
TCAGCTGTGC TGCCTCGGGC TGTGATGGGG CTGGCTGCCT TGGGGCTCGT GCTGCTGCTC 
TGGAGGCCTG TGACAGTGCT GGTGAAAGCT GGGGCTGCTA CTTCAAGGAC CAGGACTATC 
CTCACTCCCT TCTCAGGTCC CCCTACTTCT CAGGCTGACC TCGACTATGT AGTCCCACAA 
ATCTACCGCC ACATGCAGGA GGAGTTCCAG GGCCGGCTCG AGAGGACTAA AGCTCAGGGT 
CCCATAACCG TGGCTGCCTA TCAGTTGGGA AGCGTGTACT CGGCTGCTAT GGTCACGGCC 
CTCCTCCTCT TGGCCTTCCC ACTTATGCTG TTGCATGTAG AGCGGGTCAG CCTGGTGTTC 
CTGCTCCTGT TTCTGCAGAG CTTCCTTCTC CTGCATCTGC TGGCCGCTGG GACACCAGTC 
GCCACCCCTG GCCCTTTTAC TGTGCTGTGG CAGGCAGTCT CAGCTTGGGT CCTTCTGGCT 
ACCCAGACCT TCTACTCCAC AGGCCACCAG CCTGTCTTTT CAGCCATCCA TTGGCACGCA 
GCCTTTGTGG GCTTCCCAGA CGGCCATGGC TCTTCTACCT GGTTGCCTGC CTTACTAGTG 
GGAGCTAACA CCTTTGCCTC CCACCTCCTC TTCGCAGTAG GCTGCCCACT GCTCCTGCTC 
TGGCCCTTCC TGTGTGAGCG TCAAGGGCCA AAGAGAAGGC AGCCCCTCCC GGGAAGTGAG 
TCTGAAGCCA GAGTCCGGCC TGAGGAAGAG GAGGAGCTGC AGGAGCCACT GATGGAGATG 
CGCCTCCGGG ATGCTCCGAA TCACTTCAAT GCAGCCCTGT TGCAGCTGGG CCTCAAGTAC 
CTCTTTATCC TTGGAGCTCA GATTCTAGCT TGTGCCTTGG CAGCTTCCAT CCTCCGCAGG 
CACCTCATGG TCTGGAAGGT GTTTGCTCCC AAGTTCATTT TTGAAGCCGT AGGTTTCATC 
GTGAGCAGTG TGGGACTTCT CCTGGGCATA GCATTGGTGA TGCGAGTGGA TGTCGCTGTG 
AGTTCCTGGT TCAAAAAACT AGTTCTGGCC CAGCAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pigo ( NM_020035.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064419.2 Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant 1, mRNA.

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CloneID	OMu11824
Clone ID Related Accession (Same CDS sequence)	NM_020035.3
Accession Version	NM_020035.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	GPI ethanolamine phosphate transferase 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC092394.1 and BU708685.1. On Mar 10, 2015 this sequence version replaced NM_020035.2. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC092394.1, AB038560.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACATTC TCTTCTCTCC CAGGATGCGG AAGGTGTCTG TGCTCCTCTT CTTAGCCTGG 
GTCTGTTTTC TTTTCTACGC TGGCATTGCA CTCTTCACCA GTGGCTTCCT TCTCACTCGT 
CTGGAGCTCA CCAATCAAAG CAGCTGCCAA GAGCTCCCAG GCCCTGGTCC CTTGCCGTGG 
GGAAGTCATG GAAAGCCTGG GGCTTGCTGG ATGCCTTCCC GCTTCTCCAG GGTGGTTTTG 
GTACTGATAG ACGCTCTGCG GTTTGACTTT GCCCAGCCTC AGCGCTCGCA TGTGCCTGGG 
GAACCTCCTG TCTCCGTGCC TTTTCTGGGA AAATTAGGTT CCTTGCAGAG GATCCTAGAG 
AGTCAACCCC ACCATGGCCG GCTGTACCGA TCTCAGGTGG ACCCCCCCAC GACCACCATG 
CAGCGTCTAA AGGCTCTCAC CACTGGCTCG CTGCCGACTT TCATTGATGC TGGCAGTAAC 
TTTGCCAGCC ATGCTATAGT GGAAGATAAT GTCATCCAAC AGCTCAACAG TGCAGGAAGG 
CGTGTAGTCT TCATGGGAGA TGATACCTGG AGAGACCTCT TCCCCGGAGC CTTTTCTCAA 
GCTTTCTTCT TCTCATCCTT CAATGTCAGA GACCTGCACA CAGTGGATAA CGGCATCTTG 
GAACACCTCT ATCCTACCCT GGACGGCGGT TCGTGGGATG TGCTGATCGC TCACTTCCTG 
GGTGTGGATC ACTGTGGTCA CAAGCACGGC CCTCACCACC CTGAAATGGC CAAGAAACTT 
AGCCAGATGG ACCAGGTGAT ACAGGGACTC ATAGAACGCC TGGAGAATGA CACACTGCTG 
GTGGTAGCCG GTGACCATGG GATGACCATG AATGGGGACC ATGGAGGGGA CAGTGAGCTG 
GAGGTGTCAG CTGCACTTTT CCTGTACAGC CCCACAGCTC TCTTCCCTAG TGTCCCACCC 
GAGGAGCCGG AGGTTATTCC TCAGGTCAGC CTTGTGCCCA CGCTGGCACT GCTGCTAGGC 
CTGCCCATCC CGTTTGGGAA CACTGGGGAA GTGATGGCAG AGCTATTCTC AGGTGGCAGT 
GACTCCTCCC ATCCTCACTC CTCTGCGCTA GCCCAAGTCT CGGCTCTCCA CATCAATGCC 
CAGCAGGTGT CCCGGTTTCT TCATACCTAC TCGGCTGCGA CTCAGGATCT CCAAGTTAAA 
GAACTTCATC GGCTGCAGAC GCTCTTCTCC AAGGCCTCTG CGCGTTACCA GCACTTTCTG 
CGGGATCCCC AAGAGGCTGA GGCAGCGCTG AGCACATTGA CTGCTGAGTT CCAGCAGTTC 
CTGCGGGGAG CTCGGGCCCT GTGCATCGAG TCTTGGGCCC GCTTCTCTCT GGTCCGAATG 
GCAGGGGGCG CTGCTCTTTT GGCTGCTGCC TGCCTTCTCT GCCTGCTTGC TTCCCAGTTG 
GCAGTCGCCC CAGGCTTTCT CTTCCGCCCA CTACTGCTAA TACCTGTAGC CTGGGGCCTA 
ACTTGGACCA TCCTGTATGC TGGAGTCTCG GTAACTACTG GGTCGAAGAT AGACTTAGTG 
GTTCTGGGGG CTGTGGCTGC AGCCGGTTCA CTCCTCCCTT TTCTGTGGAA AGCTTGGGTT 
AGCAGGGGGT CTAAGAGGCC ACTGGCTCCC CTACTTCCTG TCCCTAGACC TGTCCTAATA 
TTACTGCTCA TTCGCTTGGC CACCTTCTTC TCGGATAGCT TTGTTGTTGC TGAGGCCAGG 
GCCACCCCCT TCCTTCTGGG CTCTCTCGTT TTCTTCCTGG TTGCTCAGCT CCACTGGGAG 
GGTCAGCTAC TGCCACCCAA ACCACTCACG ATGTCCCGCC TTGGCTCTTC TGCCCCAACA 
GCGCCCCCAC GGCACAGTGG CGCGCATGCC CTGTGGCTTG GAATTGGGTT GCTTTTATTT 
ACAAGGCTCG CTGGGCTTTT TCACCGCTGC CCGGAAGAGA CACCTGCCTG TCGCTCCTCT 
CCCTGGCTGA GTCCTCTGGC ATCTATGGTG GGAGGTCGAG CCAAGAATTT GTGGTACGGA 
GCTTGCGTGG GGGCACTGGT GGCCCTGTTA GTCGTCGTGC GCTTGTGGCT TCGCCGCTAT 
GGTAATCTCA AGAGTCCTGA GCCCCCTGTG CTCTTTGTGC GCTGGGGGAT GCCTCTCATG 
GTACTGGGCA CGGCTGCCTA CTGGGCACTG GCATCAGGTG CAGAGGAAGC ACCACCACGC 
CTCCGGGCCC TGGTCGCCGG GGCATCAGCT GTGCTGCCTC GGGCTGTGAT GGGGCTGGCT 
GCCTTGGGGC TCGTGCTGCT GCTCTGGAGG CCTGTGACAG TGCTGGTGAA AGCTGGGGCT 
GCTACTTCAA GGACCAGGAC TATCCTCACT CCCTTCTCAG GTCCCCCTAC TTCTCAGGCT 
GACCTCGACT ATGTAGTCCC ACAAATCTAC CGCCACATGC AGGAGGAGTT CCAGGGCCGG 
CTCGAGAGGA CTAAAGCTCA GGGTCCCATA ACCGTGGCTG CCTATCAGTT GGGAAGCGTG 
TACTCGGCTG CTATGGTCAC GGCCCTCCTC CTCTTGGCCT TCCCACTTAT GCTGTTGCAT 
GTAGAGCGGG TCAGCCTGGT GTTCCTGCTC CTGTTTCTGC AGAGCTTCCT TCTCCTGCAT 
CTGCTGGCCG CTGGGACACC AGTCGCCACC CCTGGCCCTT TTACTGTGCT GTGGCAGGCA 
GTCTCAGCTT GGGTCCTTCT GGCTACCCAG ACCTTCTACT CCACAGGCCA CCAGCCTGTC 
TTTTCAGCCA TCCATTGGCA CGCAGCCTTT GTGGGCTTCC CAGACGGCCA TGGCTCTTCT 
ACCTGGTTGC CTGCCTTACT AGTGGGAGCT AACACCTTTG CCTCCCACCT CCTCTTCGCA 
GTAGGCTGCC CACTGCTCCT GCTCTGGCCC TTCCTGTGTG AGCGTCAAGG GCCAAAGAGA 
AGGCAGCCCC TCCCGGGAAG TGAGTCTGAA GCCAGAGTCC GGCCTGAGGA AGAGGAGGAG 
CTGCAGGAGC CACTGATGGA GATGCGCCTC CGGGATGCTC CGAATCACTT CAATGCAGCC 
CTGTTGCAGC TGGGCCTCAA GTACCTCTTT ATCCTTGGAG CTCAGATTCT AGCTTGTGCC 
TTGGCAGCTT CCATCCTCCG CAGGCACCTC ATGGTCTGGA AGGTGTTTGC TCCCAAGTTC 
ATTTTTGAAG CCGTAGGTTT CATCGTGAGC AGTGTGGGAC TTCTCCTGGG CATAGCATTG 
GTGATGCGAG TGGATGTCGC TGTGAGTTCC TGGTTCAAAA AACTAGTTCT GGCCCAGCAG 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064419.2
CDS	678..3983
Translation	MNILFSPRMRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLASQLAVAPGFLFRPLLLIPVAWGLTWTILYAGVSVTTGSKIDLVVLGAVAAAGSLLPFLWKAWVSRGSKRPLAPLLPVPRPVLILLLIRLATFFSDSFVVAEARATPFLLGSLVFFLVAQLHWEGQLLPPKPLTMSRLGSSAPTAPPRHSGAHALWLGIGLLLFTRLAGLFHRCPEETPACRSSPWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPEPPVLFVRWGMPLMVLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWRPVTVLVKAGAATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSVYSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPFTVLWQAVSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEPLMEMRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR

Target ORF information:

RefSeq Version	NM_020035.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020035.3

ORF Insert Sequence:

ATGAACATTC TCTTCTCTCC CAGGATGCGG AAGGTGTCTG TGCTCCTCTT CTTAGCCTGG 
GTCTGTTTTC TTTTCTACGC TGGCATTGCA CTCTTCACCA GTGGCTTCCT TCTCACTCGT 
CTGGAGCTCA CCAATCAAAG CAGCTGCCAA GAGCTCCCAG GCCCTGGTCC CTTGCCGTGG 
GGAAGTCATG GAAAGCCTGG GGCTTGCTGG ATGCCTTCCC GCTTCTCCAG GGTGGTTTTG 
GTACTGATAG ACGCTCTGCG GTTTGACTTT GCCCAGCCTC AGCGCTCGCA TGTGCCTGGG 
GAACCTCCTG TCTCCGTGCC TTTTCTGGGA AAATTAGGTT CCTTGCAGAG GATCCTAGAG 
AGTCAACCCC ACCATGGCCG GCTGTACCGA TCTCAGGTGG ACCCCCCCAC GACCACCATG 
CAGCGTCTAA AGGCTCTCAC CACTGGCTCG CTGCCGACTT TCATTGATGC TGGCAGTAAC 
TTTGCCAGCC ATGCTATAGT GGAAGATAAT GTCATCCAAC AGCTCAACAG TGCAGGAAGG 
CGTGTAGTCT TCATGGGAGA TGATACCTGG AGAGACCTCT TCCCCGGAGC CTTTTCTCAA 
GCTTTCTTCT TCTCATCCTT CAATGTCAGA GACCTGCACA CAGTGGATAA CGGCATCTTG 
GAACACCTCT ATCCTACCCT GGACGGCGGT TCGTGGGATG TGCTGATCGC TCACTTCCTG 
GGTGTGGATC ACTGTGGTCA CAAGCACGGC CCTCACCACC CTGAAATGGC CAAGAAACTT 
AGCCAGATGG ACCAGGTGAT ACAGGGACTC ATAGAACGCC TGGAGAATGA CACACTGCTG 
GTGGTAGCCG GTGACCATGG GATGACCATG AATGGGGACC ATGGAGGGGA CAGTGAGCTG 
GAGGTGTCAG CTGCACTTTT CCTGTACAGC CCCACAGCTC TCTTCCCTAG TGTCCCACCC 
GAGGAGCCGG AGGTTATTCC TCAGGTCAGC CTTGTGCCCA CGCTGGCACT GCTGCTAGGC 
CTGCCCATCC CGTTTGGGAA CACTGGGGAA GTGATGGCAG AGCTATTCTC AGGTGGCAGT 
GACTCCTCCC ATCCTCACTC CTCTGCGCTA GCCCAAGTCT CGGCTCTCCA CATCAATGCC 
CAGCAGGTGT CCCGGTTTCT TCATACCTAC TCGGCTGCGA CTCAGGATCT CCAAGTTAAA 
GAACTTCATC GGCTGCAGAC GCTCTTCTCC AAGGCCTCTG CGCGTTACCA GCACTTTCTG 
CGGGATCCCC AAGAGGCTGA GGCAGCGCTG AGCACATTGA CTGCTGAGTT CCAGCAGTTC 
CTGCGGGGAG CTCGGGCCCT GTGCATCGAG TCTTGGGCCC GCTTCTCTCT GGTCCGAATG 
GCAGGGGGCG CTGCTCTTTT GGCTGCTGCC TGCCTTCTCT GCCTGCTTGC TTCCCAGTTG 
GCAGTCGCCC CAGGCTTTCT CTTCCGCCCA CTACTGCTAA TACCTGTAGC CTGGGGCCTA 
ACTTGGACCA TCCTGTATGC TGGAGTCTCG GTAACTACTG GGTCGAAGAT AGACTTAGTG 
GTTCTGGGGG CTGTGGCTGC AGCCGGTTCA CTCCTCCCTT TTCTGTGGAA AGCTTGGGTT 
AGCAGGGGGT CTAAGAGGCC ACTGGCTCCC CTACTTCCTG TCCCTAGACC TGTCCTAATA 
TTACTGCTCA TTCGCTTGGC CACCTTCTTC TCGGATAGCT TTGTTGTTGC TGAGGCCAGG 
GCCACCCCCT TCCTTCTGGG CTCTCTCGTT TTCTTCCTGG TTGCTCAGCT CCACTGGGAG 
GGTCAGCTAC TGCCACCCAA ACCACTCACG ATGTCCCGCC TTGGCTCTTC TGCCCCAACA 
GCGCCCCCAC GGCACAGTGG CGCGCATGCC CTGTGGCTTG GAATTGGGTT GCTTTTATTT 
ACAAGGCTCG CTGGGCTTTT TCACCGCTGC CCGGAAGAGA CACCTGCCTG TCGCTCCTCT 
CCCTGGCTGA GTCCTCTGGC ATCTATGGTG GGAGGTCGAG CCAAGAATTT GTGGTACGGA 
GCTTGCGTGG GGGCACTGGT GGCCCTGTTA GTCGTCGTGC GCTTGTGGCT TCGCCGCTAT 
GGTAATCTCA AGAGTCCTGA GCCCCCTGTG CTCTTTGTGC GCTGGGGGAT GCCTCTCATG 
GTACTGGGCA CGGCTGCCTA CTGGGCACTG GCATCAGGTG CAGAGGAAGC ACCACCACGC 
CTCCGGGCCC TGGTCGCCGG GGCATCAGCT GTGCTGCCTC GGGCTGTGAT GGGGCTGGCT 
GCCTTGGGGC TCGTGCTGCT GCTCTGGAGG CCTGTGACAG TGCTGGTGAA AGCTGGGGCT 
GCTACTTCAA GGACCAGGAC TATCCTCACT CCCTTCTCAG GTCCCCCTAC TTCTCAGGCT 
GACCTCGACT ATGTAGTCCC ACAAATCTAC CGCCACATGC AGGAGGAGTT CCAGGGCCGG 
CTCGAGAGGA CTAAAGCTCA GGGTCCCATA ACCGTGGCTG CCTATCAGTT GGGAAGCGTG 
TACTCGGCTG CTATGGTCAC GGCCCTCCTC CTCTTGGCCT TCCCACTTAT GCTGTTGCAT 
GTAGAGCGGG TCAGCCTGGT GTTCCTGCTC CTGTTTCTGC AGAGCTTCCT TCTCCTGCAT 
CTGCTGGCCG CTGGGACACC AGTCGCCACC CCTGGCCCTT TTACTGTGCT GTGGCAGGCA 
GTCTCAGCTT GGGTCCTTCT GGCTACCCAG ACCTTCTACT CCACAGGCCA CCAGCCTGTC 
TTTTCAGCCA TCCATTGGCA CGCAGCCTTT GTGGGCTTCC CAGACGGCCA TGGCTCTTCT 
ACCTGGTTGC CTGCCTTACT AGTGGGAGCT AACACCTTTG CCTCCCACCT CCTCTTCGCA 
GTAGGCTGCC CACTGCTCCT GCTCTGGCCC TTCCTGTGTG AGCGTCAAGG GCCAAAGAGA 
AGGCAGCCCC TCCCGGGAAG TGAGTCTGAA GCCAGAGTCC GGCCTGAGGA AGAGGAGGAG 
CTGCAGGAGC CACTGATGGA GATGCGCCTC CGGGATGCTC CGAATCACTT CAATGCAGCC 
CTGTTGCAGC TGGGCCTCAA GTACCTCTTT ATCCTTGGAG CTCAGATTCT AGCTTGTGCC 
TTGGCAGCTT CCATCCTCCG CAGGCACCTC ATGGTCTGGA AGGTGTTTGC TCCCAAGTTC 
ATTTTTGAAG CCGTAGGTTT CATCGTGAGC AGTGTGGGAC TTCTCCTGGG CATAGCATTG 
GTGATGCGAG TGGATGTCGC TGTGAGTTCC TGGTTCAAAA AACTAGTTCT GGCCCAGCAG 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pigo ( NM_001305386.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001292315.1 Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant 2, mRNA.

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CloneID	OMu33064
Clone ID Related Accession (Same CDS sequence)	XM_006538113.2 , XM_011250078.2 , XM_006538113.3 , XM_011250078.3 , NM_001305386.1
Accession Version	NM_001305386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	GPI ethanolamine phosphate transferase 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL672276.9, AK050819.1, BC092394.1 and BU708685.1. On Mar 10, 2015 this sequence version replaced XM_006538114.2. Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK050819.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGAAGG TGTCTGTGCT CCTCTTCTTA GCCTGGGTCT GTTTTCTTTT CTACGCTGGC 
ATTGCACTCT TCACCAGTGG CTTCCTTCTC ACTCGTCTGG AGCTCACCAA TCAAAGCAGC 
TGCCAAGAGC TCCCAGGCCC TGGTCCCTTG CCGTGGGGAA GTCATGGAAA GCCTGGGGCT 
TGCTGGATGC CTTCCCGCTT CTCCAGGGTG GTTTTGGTAC TGATAGACGC TCTGCGGTTT 
GACTTTGCCC AGCCTCAGCG CTCGCATGTG CCTGGGGAAC CTCCTGTCTC CGTGCCTTTT 
CTGGGAAAAT TAGGTTCCTT GCAGAGGATC CTAGAGAGTC AACCCCACCA TGGCCGGCTG 
TACCGATCTC AGGTGGACCC CCCCACGACC ACCATGCAGC GTCTAAAGGC TCTCACCACT 
GGCTCGCTGC CGACTTTCAT TGATGCTGGC AGTAACTTTG CCAGCCATGC TATAGTGGAA 
GATAATGTCA TCCAACAGCT CAACAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT 
ACCTGGAGAG ACCTCTTCCC CGGAGCCTTT TCTCAAGCTT TCTTCTTCTC ATCCTTCAAT 
GTCAGAGACC TGCACACAGT GGATAACGGC ATCTTGGAAC ACCTCTATCC TACCCTGGAC 
GGCGGTTCGT GGGATGTGCT GATCGCTCAC TTCCTGGGTG TGGATCACTG TGGTCACAAG 
CACGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATACAG 
GGACTCATAG AACGCCTGGA GAATGACACA CTGCTGGTGG TAGCCGGTGA CCATGGGATG 
ACCATGAATG GGGACCATGG AGGGGACAGT GAGCTGGAGG TGTCAGCTGC ACTTTTCCTG 
TACAGCCCCA CAGCTCTCTT CCCTAGTGTC CCACCCGAGG AGCCGGAGGT TATTCCTCAG 
GTCAGCCTTG TGCCCACGCT GGCACTGCTG CTAGGCCTGC CCATCCCGTT TGGGAACACT 
GGGGAAGTGA TGGCAGAGCT ATTCTCAGGT GGCAGTGACT CCTCCCATCC TCACTCCTCT 
GCGCTAGCCC AAGTCTCGGC TCTCCACATC AATGCCCAGC AGGTGTCCCG GTTTCTTCAT 
ACCTACTCGG CTGCGACTCA GGATCTCCAA GTTAAAGAAC TTCATCGGCT GCAGACGCTC 
TTCTCCAAGG CCTCTGCGCG TTACCAGCAC TTTCTGCGGG ATCCCCAAGA GGCTGAGGCA 
GCGCTGAGCA CATTGACTGC TGAGTTCCAG CAGTTCCTGC GGGGAGCTCG GGCCCTGTGC 
ATCGAGTCTT GGGCCCGCTT CTCTCTGGTC CGAATGGCAG GGGGCGCTGC TCTTTTGGCT 
GCTGCCTGCC TTCTCTGCCT GCTTGCTTCC CAGTTGGCAG TCGCCCCAGG CTTTCTCTTC 
CGCCCACTAC TGCTAATACC TGTAGCCTGG GGCCTAACTT GGACCATCCT GTATGCTGGA 
GTCTCGGTAA CTACTGGGTC GAAGATAGAC TTAGTGGTTC TGGGGGCTGT GGCTGCAGCC 
GGTTCACTCC TCCCTTTTCT GTGGAAAGCT TGGGTTAGCA GGGGGTCTAA GAGGCCACTG 
GCTCCCCTAC TTCCTGTCCC TAGACCTGTC CTAATATTAC TGCTCATTCG CTTGGCCACC 
TTCTTCTCGG ATAGCTTTGT TGTTGCTGAG GCCAGGGCCA CCCCCTTCCT TCTGGGCTCT 
CTCGTTTTCT TCCTGGTTGC TCAGCTCCAC TGGGAGGGTC AGCTACTGCC ACCCAAACCA 
CTCACGATGT CCCGCCTTGG CTCTTCTGCC CCAACAGCGC CCCCACGGCA CAGTGGCGCG 
CATGCCCTGT GGCTTGGAAT TGGGTTGCTT TTATTTACAA GGCTCGCTGG GCTTTTTCAC 
CGCTGCCCGG AAGAGACACC TGCCTGTCGC TCCTCTCCCT GGCTGAGTCC TCTGGCATCT 
ATGGTGGGAG GTCGAGCCAA GAATTTGTGG TACGGAGCTT GCGTGGGGGC ACTGGTGGCC 
CTGTTAGTCG TCGTGCGCTT GTGGCTTCGC CGCTATGGTA ATCTCAAGAG TCCTGAGCCC 
CCTGTGCTCT TTGTGCGCTG GGGGATGCCT CTCATGGTAC TGGGCACGGC TGCCTACTGG 
GCACTGGCAT CAGGTGCAGA GGAAGCACCA CCACGCCTCC GGGCCCTGGT CGCCGGGGCA 
TCAGCTGTGC TGCCTCGGGC TGTGATGGGG CTGGCTGCCT TGGGGCTCGT GCTGCTGCTC 
TGGAGGCCTG TGACAGTGCT GGTGAAAGCT GGGGCTGCTA CTTCAAGGAC CAGGACTATC 
CTCACTCCCT TCTCAGGTCC CCCTACTTCT CAGGCTGACC TCGACTATGT AGTCCCACAA 
ATCTACCGCC ACATGCAGGA GGAGTTCCAG GGCCGGCTCG AGAGGACTAA AGCTCAGGGT 
CCCATAACCG TGGCTGCCTA TCAGTTGGGA AGCGTGTACT CGGCTGCTAT GGTCACGGCC 
CTCCTCCTCT TGGCCTTCCC ACTTATGCTG TTGCATGTAG AGCGGGTCAG CCTGGTGTTC 
CTGCTCCTGT TTCTGCAGAG CTTCCTTCTC CTGCATCTGC TGGCCGCTGG GACACCAGTC 
GCCACCCCTG GCCCTTTTAC TGTGCTGTGG CAGGCAGTCT CAGCTTGGGT CCTTCTGGCT 
ACCCAGACCT TCTACTCCAC AGGCCACCAG CCTGTCTTTT CAGCCATCCA TTGGCACGCA 
GCCTTTGTGG GCTTCCCAGA CGGCCATGGC TCTTCTACCT GGTTGCCTGC CTTACTAGTG 
GGAGCTAACA CCTTTGCCTC CCACCTCCTC TTCGCAGTAG GCTGCCCACT GCTCCTGCTC 
TGGCCCTTCC TGTGTGAGCG TCAAGGGCCA AAGAGAAGGC AGCCCCTCCC GGGAAGTGAG 
TCTGAAGCCA GAGTCCGGCC TGAGGAAGAG GAGGAGCTGC AGGAGCCACT GATGGAGATG 
CGCCTCCGGG ATGCTCCGAA TCACTTCAAT GCAGCCCTGT TGCAGCTGGG CCTCAAGTAC 
CTCTTTATCC TTGGAGCTCA GATTCTAGCT TGTGCCTTGG CAGCTTCCAT CCTCCGCAGG 
CACCTCATGG TCTGGAAGGT GTTTGCTCCC AAGTTCATTT TTGAAGCCGT AGGTTTCATC 
GTGAGCAGTG TGGGACTTCT CCTGGGCATA GCATTGGTGA TGCGAGTGGA TGTCGCTGTG 
AGTTCCTGGT TCAAAAAACT AGTTCTGGCC CAGCAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292315.1
CDS	37..3318
Translation	MRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLASQLAVAPGFLFRPLLLIPVAWGLTWTILYAGVSVTTGSKIDLVVLGAVAAAGSLLPFLWKAWVSRGSKRPLAPLLPVPRPVLILLLIRLATFFSDSFVVAEARATPFLLGSLVFFLVAQLHWEGQLLPPKPLTMSRLGSSAPTAPPRHSGAHALWLGIGLLLFTRLAGLFHRCPEETPACRSSPWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPEPPVLFVRWGMPLMVLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWRPVTVLVKAGAATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSVYSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPFTVLWQAVSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEPLMEMRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR

Target ORF information:

RefSeq Version	NM_001305386.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305386.1

ORF Insert Sequence:

ATGCGGAAGG TGTCTGTGCT CCTCTTCTTA GCCTGGGTCT GTTTTCTTTT CTACGCTGGC 
ATTGCACTCT TCACCAGTGG CTTCCTTCTC ACTCGTCTGG AGCTCACCAA TCAAAGCAGC 
TGCCAAGAGC TCCCAGGCCC TGGTCCCTTG CCGTGGGGAA GTCATGGAAA GCCTGGGGCT 
TGCTGGATGC CTTCCCGCTT CTCCAGGGTG GTTTTGGTAC TGATAGACGC TCTGCGGTTT 
GACTTTGCCC AGCCTCAGCG CTCGCATGTG CCTGGGGAAC CTCCTGTCTC CGTGCCTTTT 
CTGGGAAAAT TAGGTTCCTT GCAGAGGATC CTAGAGAGTC AACCCCACCA TGGCCGGCTG 
TACCGATCTC AGGTGGACCC CCCCACGACC ACCATGCAGC GTCTAAAGGC TCTCACCACT 
GGCTCGCTGC CGACTTTCAT TGATGCTGGC AGTAACTTTG CCAGCCATGC TATAGTGGAA 
GATAATGTCA TCCAACAGCT CAACAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT 
ACCTGGAGAG ACCTCTTCCC CGGAGCCTTT TCTCAAGCTT TCTTCTTCTC ATCCTTCAAT 
GTCAGAGACC TGCACACAGT GGATAACGGC ATCTTGGAAC ACCTCTATCC TACCCTGGAC 
GGCGGTTCGT GGGATGTGCT GATCGCTCAC TTCCTGGGTG TGGATCACTG TGGTCACAAG 
CACGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATACAG 
GGACTCATAG AACGCCTGGA GAATGACACA CTGCTGGTGG TAGCCGGTGA CCATGGGATG 
ACCATGAATG GGGACCATGG AGGGGACAGT GAGCTGGAGG TGTCAGCTGC ACTTTTCCTG 
TACAGCCCCA CAGCTCTCTT CCCTAGTGTC CCACCCGAGG AGCCGGAGGT TATTCCTCAG 
GTCAGCCTTG TGCCCACGCT GGCACTGCTG CTAGGCCTGC CCATCCCGTT TGGGAACACT 
GGGGAAGTGA TGGCAGAGCT ATTCTCAGGT GGCAGTGACT CCTCCCATCC TCACTCCTCT 
GCGCTAGCCC AAGTCTCGGC TCTCCACATC AATGCCCAGC AGGTGTCCCG GTTTCTTCAT 
ACCTACTCGG CTGCGACTCA GGATCTCCAA GTTAAAGAAC TTCATCGGCT GCAGACGCTC 
TTCTCCAAGG CCTCTGCGCG TTACCAGCAC TTTCTGCGGG ATCCCCAAGA GGCTGAGGCA 
GCGCTGAGCA CATTGACTGC TGAGTTCCAG CAGTTCCTGC GGGGAGCTCG GGCCCTGTGC 
ATCGAGTCTT GGGCCCGCTT CTCTCTGGTC CGAATGGCAG GGGGCGCTGC TCTTTTGGCT 
GCTGCCTGCC TTCTCTGCCT GCTTGCTTCC CAGTTGGCAG TCGCCCCAGG CTTTCTCTTC 
CGCCCACTAC TGCTAATACC TGTAGCCTGG GGCCTAACTT GGACCATCCT GTATGCTGGA 
GTCTCGGTAA CTACTGGGTC GAAGATAGAC TTAGTGGTTC TGGGGGCTGT GGCTGCAGCC 
GGTTCACTCC TCCCTTTTCT GTGGAAAGCT TGGGTTAGCA GGGGGTCTAA GAGGCCACTG 
GCTCCCCTAC TTCCTGTCCC TAGACCTGTC CTAATATTAC TGCTCATTCG CTTGGCCACC 
TTCTTCTCGG ATAGCTTTGT TGTTGCTGAG GCCAGGGCCA CCCCCTTCCT TCTGGGCTCT 
CTCGTTTTCT TCCTGGTTGC TCAGCTCCAC TGGGAGGGTC AGCTACTGCC ACCCAAACCA 
CTCACGATGT CCCGCCTTGG CTCTTCTGCC CCAACAGCGC CCCCACGGCA CAGTGGCGCG 
CATGCCCTGT GGCTTGGAAT TGGGTTGCTT TTATTTACAA GGCTCGCTGG GCTTTTTCAC 
CGCTGCCCGG AAGAGACACC TGCCTGTCGC TCCTCTCCCT GGCTGAGTCC TCTGGCATCT 
ATGGTGGGAG GTCGAGCCAA GAATTTGTGG TACGGAGCTT GCGTGGGGGC ACTGGTGGCC 
CTGTTAGTCG TCGTGCGCTT GTGGCTTCGC CGCTATGGTA ATCTCAAGAG TCCTGAGCCC 
CCTGTGCTCT TTGTGCGCTG GGGGATGCCT CTCATGGTAC TGGGCACGGC TGCCTACTGG 
GCACTGGCAT CAGGTGCAGA GGAAGCACCA CCACGCCTCC GGGCCCTGGT CGCCGGGGCA 
TCAGCTGTGC TGCCTCGGGC TGTGATGGGG CTGGCTGCCT TGGGGCTCGT GCTGCTGCTC 
TGGAGGCCTG TGACAGTGCT GGTGAAAGCT GGGGCTGCTA CTTCAAGGAC CAGGACTATC 
CTCACTCCCT TCTCAGGTCC CCCTACTTCT CAGGCTGACC TCGACTATGT AGTCCCACAA 
ATCTACCGCC ACATGCAGGA GGAGTTCCAG GGCCGGCTCG AGAGGACTAA AGCTCAGGGT 
CCCATAACCG TGGCTGCCTA TCAGTTGGGA AGCGTGTACT CGGCTGCTAT GGTCACGGCC 
CTCCTCCTCT TGGCCTTCCC ACTTATGCTG TTGCATGTAG AGCGGGTCAG CCTGGTGTTC 
CTGCTCCTGT TTCTGCAGAG CTTCCTTCTC CTGCATCTGC TGGCCGCTGG GACACCAGTC 
GCCACCCCTG GCCCTTTTAC TGTGCTGTGG CAGGCAGTCT CAGCTTGGGT CCTTCTGGCT 
ACCCAGACCT TCTACTCCAC AGGCCACCAG CCTGTCTTTT CAGCCATCCA TTGGCACGCA 
GCCTTTGTGG GCTTCCCAGA CGGCCATGGC TCTTCTACCT GGTTGCCTGC CTTACTAGTG 
GGAGCTAACA CCTTTGCCTC CCACCTCCTC TTCGCAGTAG GCTGCCCACT GCTCCTGCTC 
TGGCCCTTCC TGTGTGAGCG TCAAGGGCCA AAGAGAAGGC AGCCCCTCCC GGGAAGTGAG 
TCTGAAGCCA GAGTCCGGCC TGAGGAAGAG GAGGAGCTGC AGGAGCCACT GATGGAGATG 
CGCCTCCGGG ATGCTCCGAA TCACTTCAAT GCAGCCCTGT TGCAGCTGGG CCTCAAGTAC 
CTCTTTATCC TTGGAGCTCA GATTCTAGCT TGTGCCTTGG CAGCTTCCAT CCTCCGCAGG 
CACCTCATGG TCTGGAAGGT GTTTGCTCCC AAGTTCATTT TTGAAGCCGT AGGTTTCATC 
GTGAGCAGTG TGGGACTTCT CCTGGGCATA GCATTGGTGA TGCGAGTGGA TGTCGCTGTG 
AGTTCCTGGT TCAAAAAACT AGTTCTGGCC CAGCAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pigo ( NM_001305387.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001292316.1 Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant 3, mRNA.

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CloneID	OMu69674
Clone ID Related Accession (Same CDS sequence)	NM_001305387.1
Accession Version	NM_001305387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	GPI ethanolamine phosphate transferase 3 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF532064.1, BY130204.1, BC092394.1 and BU708685.1. Transcript Variant: This variant (3) differs in the 5' UTR, uses a downstream start codon, and uses an alternate in-frame splice site in 3' coding region compared to variant 1. The encoded isoform (3) has a shorter N-terminus and lacks an internal segment compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC036977.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGAAGG TGTCTGTGCT CCTCTTCTTA GCCTGGGTCT GTTTTCTTTT CTACGCTGGC 
ATTGCACTCT TCACCAGTGG CTTCCTTCTC ACTCGTCTGG AGCTCACCAA TCAAAGCAGC 
TGCCAAGAGC TCCCAGGCCC TGGTCCCTTG CCGTGGGGAA GTCATGGAAA GCCTGGGGCT 
TGCTGGATGC CTTCCCGCTT CTCCAGGGTG GTTTTGGTAC TGATAGACGC TCTGCGGTTT 
GACTTTGCCC AGCCTCAGCG CTCGCATGTG CCTGGGGAAC CTCCTGTCTC CGTGCCTTTT 
CTGGGAAAAT TAGGTTCCTT GCAGAGGATC CTAGAGAGTC AACCCCACCA TGGCCGGCTG 
TACCGATCTC AGGTGGACCC CCCCACGACC ACCATGCAGC GTCTAAAGGC TCTCACCACT 
GGCTCGCTGC CGACTTTCAT TGATGCTGGC AGTAACTTTG CCAGCCATGC TATAGTGGAA 
GATAATGTCA TCCAACAGCT CAACAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT 
ACCTGGAGAG ACCTCTTCCC CGGAGCCTTT TCTCAAGCTT TCTTCTTCTC ATCCTTCAAT 
GTCAGAGACC TGCACACAGT GGATAACGGC ATCTTGGAAC ACCTCTATCC TACCCTGGAC 
GGCGGTTCGT GGGATGTGCT GATCGCTCAC TTCCTGGGTG TGGATCACTG TGGTCACAAG 
CACGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATACAG 
GGACTCATAG AACGCCTGGA GAATGACACA CTGCTGGTGG TAGCCGGTGA CCATGGGATG 
ACCATGAATG GGGACCATGG AGGGGACAGT GAGCTGGAGG TGTCAGCTGC ACTTTTCCTG 
TACAGCCCCA CAGCTCTCTT CCCTAGTGTC CCACCCGAGG AGCCGGAGGT TATTCCTCAG 
GTCAGCCTTG TGCCCACGCT GGCACTGCTG CTAGGCCTGC CCATCCCGTT TGGGAACACT 
GGGGAAGTGA TGGCAGAGCT ATTCTCAGGT GGCAGTGACT CCTCCCATCC TCACTCCTCT 
GCGCTAGCCC AAGTCTCGGC TCTCCACATC AATGCCCAGC AGGTGTCCCG GTTTCTTCAT 
ACCTACTCGG CTGCGACTCA GGATCTCCAA GTTAAAGAAC TTCATCGGCT GCAGACGCTC 
TTCTCCAAGG CCTCTGCGCG TTACCAGCAC TTTCTGCGGG ATCCCCAAGA GGCTGAGGCA 
GCGCTGAGCA CATTGACTGC TGAGTTCCAG CAGTTCCTGC GGGGAGCTCG GGCCCTGTGC 
ATCGAGTCTT GGGCCCGCTT CTCTCTGGTC CGAATGGCAG GGGGCGCTGC TCTTTTGGCT 
GCTGCCTGCC TTCTCTGCCT GCTTGCTTCC CAGTTGGCAG TCGCCCCAGG CTTTCTCTTC 
CGCCCACTAC TGCTAATACC TGTAGCCTGG GGCCTAACTT GGACCATCCT GTATGCTGGA 
GTCTCGGTAA CTACTGGGTC GAAGATAGAC TTAGTGGTTC TGGGGGCTGT GGCTGCAGCC 
GGTTCACTCC TCCCTTTTCT GTGGAAAGCT TGGGTTAGCA GGGGGTCTAA GAGGCCACTG 
GCTCCCCTAC TTCCTGTCCC TAGACCTGTC CTAATATTAC TGCTCATTCG CTTGGCCACC 
TTCTTCTCGG ATAGCTTTGT TGTTGCTGAG GCCAGGGCCA CCCCCTTCCT TCTGGGCTCT 
CTCGTTTTCT TCCTGGTTGC TCAGCTCCAC TGGGAGGGTC AGCTACTGCC ACCCAAACCA 
CTCACGATGT CCCGCCTTGG CTCTTCTGCC CCAACAGCGC CCCCACGGCA CAGTGGCGCG 
CATGCCCTGT GGCTTGGAAT TGGGTTGCTT TTATTTACAA GGCTCGCTGG GCTTTTTCAC 
CGCTGCCCGG AAGAGACACC TGCCTGTCGC TCCTCTCCCT GGCTGAGTCC TCTGGCATCT 
ATGGTGGGAG GTCGAGCCAA GAATTTGTGG TACGGAGCTT GCGTGGGGGC ACTGGTGGCC 
CTGTTAGTCG TCGTGCGCTT GTGGCTTCGC CGCTATGGTA ATCTCAAGAG TCCTGAGCCC 
CCTGTGCTCT TTGTGCGCTG GGGGATGCCT CTCATGGTAC TGGGCACGGC TGCCTACTGG 
GCACTGGCAT CAGGTGCAGA GGAAGCACCA CCACGCCTCC GGGCCCTGGT CGCCGGGGCA 
TCAGCTGTGC TGCCTCGGGC TGTGATGGGG CTGGCTGCCT TGGGGCTCGT GCTGCTGCTC 
TGGAGGCCTG TGACAGTGCT GGTGAAAGCT GGGGCTGCTA CTTCAAGGAC CAGGACTATC 
CTCACTCCCT TCTCAGGTCC CCCTACTTCT CAGGCTGACC TCGACTATGT AGTCCCACAA 
ATCTACCGCC ACATGCAGGA GGAGTTCCAG GGCCGGCTCG AGAGGACTAA AGCTCAGGGT 
CCCATAACCG TGGCTGCCTA TCAGTTGGGA AGCGTGTACT CGGCTGCTAT GGTCACGGCC 
CTCCTCCTCT TGGCCTTCCC ACTTATGCTG TTGCATGTAG AGCGGGTCAG CCTGGTGTTC 
CTGCTCCTGT TTCTGCAGAG CTTCCTTCTC CTGCATCTGC TGGCCGCTGG GACACCAGTC 
GCCACCCCTG GCCACCAGCC TGTCTTTTCA GCCATCCATT GGCACGCAGC CTTTGTGGGC 
TTCCCAGACG GCCATGGCTC TTCTACCTGG TTGCCTGCCT TACTAGTGGG AGCTAACACC 
TTTGCCTCCC ACCTCCTCTT CGCAGTAGGC TGCCCACTGC TCCTGCTCTG GCCCTTCCTG 
TGTGAGCGTC AAGGGCCAAA GAGAAGGCAG CCCCTCCCGG GAAGTGAGTC TGAAGCCAGA 
GTCCGGCCTG AGGAAGAGGA GGAGCTGCAG GAGCCACTGA TGGAGATGCG CCTCCGGGAT 
GCTCCGAATC ACTTCAATGC AGCCCTGTTG CAGCTGGGCC TCAAGTACCT CTTTATCCTT 
GGAGCTCAGA TTCTAGCTTG TGCCTTGGCA GCTTCCATCC TCCGCAGGCA CCTCATGGTC 
TGGAAGGTGT TTGCTCCCAA GTTCATTTTT GAAGCCGTAG GTTTCATCGT GAGCAGTGTG 
GGACTTCTCC TGGGCATAGC ATTGGTGATG CGAGTGGATG TCGCTGTGAG TTCCTGGTTC 
AAAAAACTAG TTCTGGCCCA GCAGAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292316.1
CDS	47..3256
Translation	MRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLASQLAVAPGFLFRPLLLIPVAWGLTWTILYAGVSVTTGSKIDLVVLGAVAAAGSLLPFLWKAWVSRGSKRPLAPLLPVPRPVLILLLIRLATFFSDSFVVAEARATPFLLGSLVFFLVAQLHWEGQLLPPKPLTMSRLGSSAPTAPPRHSGAHALWLGIGLLLFTRLAGLFHRCPEETPACRSSPWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPEPPVLFVRWGMPLMVLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWRPVTVLVKAGAATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSVYSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEPLMEMRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR

Target ORF information:

RefSeq Version	NM_001305387.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305387.1

ORF Insert Sequence:

ATGCGGAAGG TGTCTGTGCT CCTCTTCTTA GCCTGGGTCT GTTTTCTTTT CTACGCTGGC 
ATTGCACTCT TCACCAGTGG CTTCCTTCTC ACTCGTCTGG AGCTCACCAA TCAAAGCAGC 
TGCCAAGAGC TCCCAGGCCC TGGTCCCTTG CCGTGGGGAA GTCATGGAAA GCCTGGGGCT 
TGCTGGATGC CTTCCCGCTT CTCCAGGGTG GTTTTGGTAC TGATAGACGC TCTGCGGTTT 
GACTTTGCCC AGCCTCAGCG CTCGCATGTG CCTGGGGAAC CTCCTGTCTC CGTGCCTTTT 
CTGGGAAAAT TAGGTTCCTT GCAGAGGATC CTAGAGAGTC AACCCCACCA TGGCCGGCTG 
TACCGATCTC AGGTGGACCC CCCCACGACC ACCATGCAGC GTCTAAAGGC TCTCACCACT 
GGCTCGCTGC CGACTTTCAT TGATGCTGGC AGTAACTTTG CCAGCCATGC TATAGTGGAA 
GATAATGTCA TCCAACAGCT CAACAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT 
ACCTGGAGAG ACCTCTTCCC CGGAGCCTTT TCTCAAGCTT TCTTCTTCTC ATCCTTCAAT 
GTCAGAGACC TGCACACAGT GGATAACGGC ATCTTGGAAC ACCTCTATCC TACCCTGGAC 
GGCGGTTCGT GGGATGTGCT GATCGCTCAC TTCCTGGGTG TGGATCACTG TGGTCACAAG 
CACGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATACAG 
GGACTCATAG AACGCCTGGA GAATGACACA CTGCTGGTGG TAGCCGGTGA CCATGGGATG 
ACCATGAATG GGGACCATGG AGGGGACAGT GAGCTGGAGG TGTCAGCTGC ACTTTTCCTG 
TACAGCCCCA CAGCTCTCTT CCCTAGTGTC CCACCCGAGG AGCCGGAGGT TATTCCTCAG 
GTCAGCCTTG TGCCCACGCT GGCACTGCTG CTAGGCCTGC CCATCCCGTT TGGGAACACT 
GGGGAAGTGA TGGCAGAGCT ATTCTCAGGT GGCAGTGACT CCTCCCATCC TCACTCCTCT 
GCGCTAGCCC AAGTCTCGGC TCTCCACATC AATGCCCAGC AGGTGTCCCG GTTTCTTCAT 
ACCTACTCGG CTGCGACTCA GGATCTCCAA GTTAAAGAAC TTCATCGGCT GCAGACGCTC 
TTCTCCAAGG CCTCTGCGCG TTACCAGCAC TTTCTGCGGG ATCCCCAAGA GGCTGAGGCA 
GCGCTGAGCA CATTGACTGC TGAGTTCCAG CAGTTCCTGC GGGGAGCTCG GGCCCTGTGC 
ATCGAGTCTT GGGCCCGCTT CTCTCTGGTC CGAATGGCAG GGGGCGCTGC TCTTTTGGCT 
GCTGCCTGCC TTCTCTGCCT GCTTGCTTCC CAGTTGGCAG TCGCCCCAGG CTTTCTCTTC 
CGCCCACTAC TGCTAATACC TGTAGCCTGG GGCCTAACTT GGACCATCCT GTATGCTGGA 
GTCTCGGTAA CTACTGGGTC GAAGATAGAC TTAGTGGTTC TGGGGGCTGT GGCTGCAGCC 
GGTTCACTCC TCCCTTTTCT GTGGAAAGCT TGGGTTAGCA GGGGGTCTAA GAGGCCACTG 
GCTCCCCTAC TTCCTGTCCC TAGACCTGTC CTAATATTAC TGCTCATTCG CTTGGCCACC 
TTCTTCTCGG ATAGCTTTGT TGTTGCTGAG GCCAGGGCCA CCCCCTTCCT TCTGGGCTCT 
CTCGTTTTCT TCCTGGTTGC TCAGCTCCAC TGGGAGGGTC AGCTACTGCC ACCCAAACCA 
CTCACGATGT CCCGCCTTGG CTCTTCTGCC CCAACAGCGC CCCCACGGCA CAGTGGCGCG 
CATGCCCTGT GGCTTGGAAT TGGGTTGCTT TTATTTACAA GGCTCGCTGG GCTTTTTCAC 
CGCTGCCCGG AAGAGACACC TGCCTGTCGC TCCTCTCCCT GGCTGAGTCC TCTGGCATCT 
ATGGTGGGAG GTCGAGCCAA GAATTTGTGG TACGGAGCTT GCGTGGGGGC ACTGGTGGCC 
CTGTTAGTCG TCGTGCGCTT GTGGCTTCGC CGCTATGGTA ATCTCAAGAG TCCTGAGCCC 
CCTGTGCTCT TTGTGCGCTG GGGGATGCCT CTCATGGTAC TGGGCACGGC TGCCTACTGG 
GCACTGGCAT CAGGTGCAGA GGAAGCACCA CCACGCCTCC GGGCCCTGGT CGCCGGGGCA 
TCAGCTGTGC TGCCTCGGGC TGTGATGGGG CTGGCTGCCT TGGGGCTCGT GCTGCTGCTC 
TGGAGGCCTG TGACAGTGCT GGTGAAAGCT GGGGCTGCTA CTTCAAGGAC CAGGACTATC 
CTCACTCCCT TCTCAGGTCC CCCTACTTCT CAGGCTGACC TCGACTATGT AGTCCCACAA 
ATCTACCGCC ACATGCAGGA GGAGTTCCAG GGCCGGCTCG AGAGGACTAA AGCTCAGGGT 
CCCATAACCG TGGCTGCCTA TCAGTTGGGA AGCGTGTACT CGGCTGCTAT GGTCACGGCC 
CTCCTCCTCT TGGCCTTCCC ACTTATGCTG TTGCATGTAG AGCGGGTCAG CCTGGTGTTC 
CTGCTCCTGT TTCTGCAGAG CTTCCTTCTC CTGCATCTGC TGGCCGCTGG GACACCAGTC 
GCCACCCCTG GCCACCAGCC TGTCTTTTCA GCCATCCATT GGCACGCAGC CTTTGTGGGC 
TTCCCAGACG GCCATGGCTC TTCTACCTGG TTGCCTGCCT TACTAGTGGG AGCTAACACC 
TTTGCCTCCC ACCTCCTCTT CGCAGTAGGC TGCCCACTGC TCCTGCTCTG GCCCTTCCTG 
TGTGAGCGTC AAGGGCCAAA GAGAAGGCAG CCCCTCCCGG GAAGTGAGTC TGAAGCCAGA 
GTCCGGCCTG AGGAAGAGGA GGAGCTGCAG GAGCCACTGA TGGAGATGCG CCTCCGGGAT 
GCTCCGAATC ACTTCAATGC AGCCCTGTTG CAGCTGGGCC TCAAGTACCT CTTTATCCTT 
GGAGCTCAGA TTCTAGCTTG TGCCTTGGCA GCTTCCATCC TCCGCAGGCA CCTCATGGTC 
TGGAAGGTGT TTGCTCCCAA GTTCATTTTT GAAGCCGTAG GTTTCATCGT GAGCAGTGTG 
GGACTTCTCC TGGGCATAGC ATTGGTGATG CGAGTGGATG TCGCTGTGAG TTCCTGGTTC 
AAAAAACTAG TTCTGGCCCA GCAGAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pigo ( XM_006538113.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538176.1 Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X1, mRNA.

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CloneID	OMu33064
Clone ID Related Accession (Same CDS sequence)	XM_006538113.2 , XM_011250078.2 , XM_006538113.3 , XM_011250078.3 , NM_001305386.1
Accession Version	XM_006538113.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	GPI ethanolamine phosphate transferase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAAGG TGTCTGTGCT CCTCTTCTTA GCCTGGGTCT GTTTTCTTTT CTACGCTGGC 
ATTGCACTCT TCACCAGTGG CTTCCTTCTC ACTCGTCTGG AGCTCACCAA TCAAAGCAGC 
TGCCAAGAGC TCCCAGGCCC TGGTCCCTTG CCGTGGGGAA GTCATGGAAA GCCTGGGGCT 
TGCTGGATGC CTTCCCGCTT CTCCAGGGTG GTTTTGGTAC TGATAGACGC TCTGCGGTTT 
GACTTTGCCC AGCCTCAGCG CTCGCATGTG CCTGGGGAAC CTCCTGTCTC CGTGCCTTTT 
CTGGGAAAAT TAGGTTCCTT GCAGAGGATC CTAGAGAGTC AACCCCACCA TGGCCGGCTG 
TACCGATCTC AGGTGGACCC CCCCACGACC ACCATGCAGC GTCTAAAGGC TCTCACCACT 
GGCTCGCTGC CGACTTTCAT TGATGCTGGC AGTAACTTTG CCAGCCATGC TATAGTGGAA 
GATAATGTCA TCCAACAGCT CAACAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT 
ACCTGGAGAG ACCTCTTCCC CGGAGCCTTT TCTCAAGCTT TCTTCTTCTC ATCCTTCAAT 
GTCAGAGACC TGCACACAGT GGATAACGGC ATCTTGGAAC ACCTCTATCC TACCCTGGAC 
GGCGGTTCGT GGGATGTGCT GATCGCTCAC TTCCTGGGTG TGGATCACTG TGGTCACAAG 
CACGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATACAG 
GGACTCATAG AACGCCTGGA GAATGACACA CTGCTGGTGG TAGCCGGTGA CCATGGGATG 
ACCATGAATG GGGACCATGG AGGGGACAGT GAGCTGGAGG TGTCAGCTGC ACTTTTCCTG 
TACAGCCCCA CAGCTCTCTT CCCTAGTGTC CCACCCGAGG AGCCGGAGGT TATTCCTCAG 
GTCAGCCTTG TGCCCACGCT GGCACTGCTG CTAGGCCTGC CCATCCCGTT TGGGAACACT 
GGGGAAGTGA TGGCAGAGCT ATTCTCAGGT GGCAGTGACT CCTCCCATCC TCACTCCTCT 
GCGCTAGCCC AAGTCTCGGC TCTCCACATC AATGCCCAGC AGGTGTCCCG GTTTCTTCAT 
ACCTACTCGG CTGCGACTCA GGATCTCCAA GTTAAAGAAC TTCATCGGCT GCAGACGCTC 
TTCTCCAAGG CCTCTGCGCG TTACCAGCAC TTTCTGCGGG ATCCCCAAGA GGCTGAGGCA 
GCGCTGAGCA CATTGACTGC TGAGTTCCAG CAGTTCCTGC GGGGAGCTCG GGCCCTGTGC 
ATCGAGTCTT GGGCCCGCTT CTCTCTGGTC CGAATGGCAG GGGGCGCTGC TCTTTTGGCT 
GCTGCCTGCC TTCTCTGCCT GCTTGCTTCC CAGTTGGCAG TCGCCCCAGG CTTTCTCTTC 
CGCCCACTAC TGCTAATACC TGTAGCCTGG GGCCTAACTT GGACCATCCT GTATGCTGGA 
GTCTCGGTAA CTACTGGGTC GAAGATAGAC TTAGTGGTTC TGGGGGCTGT GGCTGCAGCC 
GGTTCACTCC TCCCTTTTCT GTGGAAAGCT TGGGTTAGCA GGGGGTCTAA GAGGCCACTG 
GCTCCCCTAC TTCCTGTCCC TAGACCTGTC CTAATATTAC TGCTCATTCG CTTGGCCACC 
TTCTTCTCGG ATAGCTTTGT TGTTGCTGAG GCCAGGGCCA CCCCCTTCCT TCTGGGCTCT 
CTCGTTTTCT TCCTGGTTGC TCAGCTCCAC TGGGAGGGTC AGCTACTGCC ACCCAAACCA 
CTCACGATGT CCCGCCTTGG CTCTTCTGCC CCAACAGCGC CCCCACGGCA CAGTGGCGCG 
CATGCCCTGT GGCTTGGAAT TGGGTTGCTT TTATTTACAA GGCTCGCTGG GCTTTTTCAC 
CGCTGCCCGG AAGAGACACC TGCCTGTCGC TCCTCTCCCT GGCTGAGTCC TCTGGCATCT 
ATGGTGGGAG GTCGAGCCAA GAATTTGTGG TACGGAGCTT GCGTGGGGGC ACTGGTGGCC 
CTGTTAGTCG TCGTGCGCTT GTGGCTTCGC CGCTATGGTA ATCTCAAGAG TCCTGAGCCC 
CCTGTGCTCT TTGTGCGCTG GGGGATGCCT CTCATGGTAC TGGGCACGGC TGCCTACTGG 
GCACTGGCAT CAGGTGCAGA GGAAGCACCA CCACGCCTCC GGGCCCTGGT CGCCGGGGCA 
TCAGCTGTGC TGCCTCGGGC TGTGATGGGG CTGGCTGCCT TGGGGCTCGT GCTGCTGCTC 
TGGAGGCCTG TGACAGTGCT GGTGAAAGCT GGGGCTGCTA CTTCAAGGAC CAGGACTATC 
CTCACTCCCT TCTCAGGTCC CCCTACTTCT CAGGCTGACC TCGACTATGT AGTCCCACAA 
ATCTACCGCC ACATGCAGGA GGAGTTCCAG GGCCGGCTCG AGAGGACTAA AGCTCAGGGT 
CCCATAACCG TGGCTGCCTA TCAGTTGGGA AGCGTGTACT CGGCTGCTAT GGTCACGGCC 
CTCCTCCTCT TGGCCTTCCC ACTTATGCTG TTGCATGTAG AGCGGGTCAG CCTGGTGTTC 
CTGCTCCTGT TTCTGCAGAG CTTCCTTCTC CTGCATCTGC TGGCCGCTGG GACACCAGTC 
GCCACCCCTG GCCCTTTTAC TGTGCTGTGG CAGGCAGTCT CAGCTTGGGT CCTTCTGGCT 
ACCCAGACCT TCTACTCCAC AGGCCACCAG CCTGTCTTTT CAGCCATCCA TTGGCACGCA 
GCCTTTGTGG GCTTCCCAGA CGGCCATGGC TCTTCTACCT GGTTGCCTGC CTTACTAGTG 
GGAGCTAACA CCTTTGCCTC CCACCTCCTC TTCGCAGTAG GCTGCCCACT GCTCCTGCTC 
TGGCCCTTCC TGTGTGAGCG TCAAGGGCCA AAGAGAAGGC AGCCCCTCCC GGGAAGTGAG 
TCTGAAGCCA GAGTCCGGCC TGAGGAAGAG GAGGAGCTGC AGGAGCCACT GATGGAGATG 
CGCCTCCGGG ATGCTCCGAA TCACTTCAAT GCAGCCCTGT TGCAGCTGGG CCTCAAGTAC 
CTCTTTATCC TTGGAGCTCA GATTCTAGCT TGTGCCTTGG CAGCTTCCAT CCTCCGCAGG 
CACCTCATGG TCTGGAAGGT GTTTGCTCCC AAGTTCATTT TTGAAGCCGT AGGTTTCATC 
GTGAGCAGTG TGGGACTTCT CCTGGGCATA GCATTGGTGA TGCGAGTGGA TGTCGCTGTG 
AGTTCCTGGT TCAAAAAACT AGTTCTGGCC CAGCAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538176.1
CDS	490..3771
Translation	MRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLASQLAVAPGFLFRPLLLIPVAWGLTWTILYAGVSVTTGSKIDLVVLGAVAAAGSLLPFLWKAWVSRGSKRPLAPLLPVPRPVLILLLIRLATFFSDSFVVAEARATPFLLGSLVFFLVAQLHWEGQLLPPKPLTMSRLGSSAPTAPPRHSGAHALWLGIGLLLFTRLAGLFHRCPEETPACRSSPWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPEPPVLFVRWGMPLMVLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWRPVTVLVKAGAATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSVYSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPFTVLWQAVSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEPLMEMRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR

Target ORF information:

RefSeq Version	XM_006538113.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538113.2

ORF Insert Sequence:

ATGCGGAAGG TGTCTGTGCT CCTCTTCTTA GCCTGGGTCT GTTTTCTTTT CTACGCTGGC 
ATTGCACTCT TCACCAGTGG CTTCCTTCTC ACTCGTCTGG AGCTCACCAA TCAAAGCAGC 
TGCCAAGAGC TCCCAGGCCC TGGTCCCTTG CCGTGGGGAA GTCATGGAAA GCCTGGGGCT 
TGCTGGATGC CTTCCCGCTT CTCCAGGGTG GTTTTGGTAC TGATAGACGC TCTGCGGTTT 
GACTTTGCCC AGCCTCAGCG CTCGCATGTG CCTGGGGAAC CTCCTGTCTC CGTGCCTTTT 
CTGGGAAAAT TAGGTTCCTT GCAGAGGATC CTAGAGAGTC AACCCCACCA TGGCCGGCTG 
TACCGATCTC AGGTGGACCC CCCCACGACC ACCATGCAGC GTCTAAAGGC TCTCACCACT 
GGCTCGCTGC CGACTTTCAT TGATGCTGGC AGTAACTTTG CCAGCCATGC TATAGTGGAA 
GATAATGTCA TCCAACAGCT CAACAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT 
ACCTGGAGAG ACCTCTTCCC CGGAGCCTTT TCTCAAGCTT TCTTCTTCTC ATCCTTCAAT 
GTCAGAGACC TGCACACAGT GGATAACGGC ATCTTGGAAC ACCTCTATCC TACCCTGGAC 
GGCGGTTCGT GGGATGTGCT GATCGCTCAC TTCCTGGGTG TGGATCACTG TGGTCACAAG 
CACGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATACAG 
GGACTCATAG AACGCCTGGA GAATGACACA CTGCTGGTGG TAGCCGGTGA CCATGGGATG 
ACCATGAATG GGGACCATGG AGGGGACAGT GAGCTGGAGG TGTCAGCTGC ACTTTTCCTG 
TACAGCCCCA CAGCTCTCTT CCCTAGTGTC CCACCCGAGG AGCCGGAGGT TATTCCTCAG 
GTCAGCCTTG TGCCCACGCT GGCACTGCTG CTAGGCCTGC CCATCCCGTT TGGGAACACT 
GGGGAAGTGA TGGCAGAGCT ATTCTCAGGT GGCAGTGACT CCTCCCATCC TCACTCCTCT 
GCGCTAGCCC AAGTCTCGGC TCTCCACATC AATGCCCAGC AGGTGTCCCG GTTTCTTCAT 
ACCTACTCGG CTGCGACTCA GGATCTCCAA GTTAAAGAAC TTCATCGGCT GCAGACGCTC 
TTCTCCAAGG CCTCTGCGCG TTACCAGCAC TTTCTGCGGG ATCCCCAAGA GGCTGAGGCA 
GCGCTGAGCA CATTGACTGC TGAGTTCCAG CAGTTCCTGC GGGGAGCTCG GGCCCTGTGC 
ATCGAGTCTT GGGCCCGCTT CTCTCTGGTC CGAATGGCAG GGGGCGCTGC TCTTTTGGCT 
GCTGCCTGCC TTCTCTGCCT GCTTGCTTCC CAGTTGGCAG TCGCCCCAGG CTTTCTCTTC 
CGCCCACTAC TGCTAATACC TGTAGCCTGG GGCCTAACTT GGACCATCCT GTATGCTGGA 
GTCTCGGTAA CTACTGGGTC GAAGATAGAC TTAGTGGTTC TGGGGGCTGT GGCTGCAGCC 
GGTTCACTCC TCCCTTTTCT GTGGAAAGCT TGGGTTAGCA GGGGGTCTAA GAGGCCACTG 
GCTCCCCTAC TTCCTGTCCC TAGACCTGTC CTAATATTAC TGCTCATTCG CTTGGCCACC 
TTCTTCTCGG ATAGCTTTGT TGTTGCTGAG GCCAGGGCCA CCCCCTTCCT TCTGGGCTCT 
CTCGTTTTCT TCCTGGTTGC TCAGCTCCAC TGGGAGGGTC AGCTACTGCC ACCCAAACCA 
CTCACGATGT CCCGCCTTGG CTCTTCTGCC CCAACAGCGC CCCCACGGCA CAGTGGCGCG 
CATGCCCTGT GGCTTGGAAT TGGGTTGCTT TTATTTACAA GGCTCGCTGG GCTTTTTCAC 
CGCTGCCCGG AAGAGACACC TGCCTGTCGC TCCTCTCCCT GGCTGAGTCC TCTGGCATCT 
ATGGTGGGAG GTCGAGCCAA GAATTTGTGG TACGGAGCTT GCGTGGGGGC ACTGGTGGCC 
CTGTTAGTCG TCGTGCGCTT GTGGCTTCGC CGCTATGGTA ATCTCAAGAG TCCTGAGCCC 
CCTGTGCTCT TTGTGCGCTG GGGGATGCCT CTCATGGTAC TGGGCACGGC TGCCTACTGG 
GCACTGGCAT CAGGTGCAGA GGAAGCACCA CCACGCCTCC GGGCCCTGGT CGCCGGGGCA 
TCAGCTGTGC TGCCTCGGGC TGTGATGGGG CTGGCTGCCT TGGGGCTCGT GCTGCTGCTC 
TGGAGGCCTG TGACAGTGCT GGTGAAAGCT GGGGCTGCTA CTTCAAGGAC CAGGACTATC 
CTCACTCCCT TCTCAGGTCC CCCTACTTCT CAGGCTGACC TCGACTATGT AGTCCCACAA 
ATCTACCGCC ACATGCAGGA GGAGTTCCAG GGCCGGCTCG AGAGGACTAA AGCTCAGGGT 
CCCATAACCG TGGCTGCCTA TCAGTTGGGA AGCGTGTACT CGGCTGCTAT GGTCACGGCC 
CTCCTCCTCT TGGCCTTCCC ACTTATGCTG TTGCATGTAG AGCGGGTCAG CCTGGTGTTC 
CTGCTCCTGT TTCTGCAGAG CTTCCTTCTC CTGCATCTGC TGGCCGCTGG GACACCAGTC 
GCCACCCCTG GCCCTTTTAC TGTGCTGTGG CAGGCAGTCT CAGCTTGGGT CCTTCTGGCT 
ACCCAGACCT TCTACTCCAC AGGCCACCAG CCTGTCTTTT CAGCCATCCA TTGGCACGCA 
GCCTTTGTGG GCTTCCCAGA CGGCCATGGC TCTTCTACCT GGTTGCCTGC CTTACTAGTG 
GGAGCTAACA CCTTTGCCTC CCACCTCCTC TTCGCAGTAG GCTGCCCACT GCTCCTGCTC 
TGGCCCTTCC TGTGTGAGCG TCAAGGGCCA AAGAGAAGGC AGCCCCTCCC GGGAAGTGAG 
TCTGAAGCCA GAGTCCGGCC TGAGGAAGAG GAGGAGCTGC AGGAGCCACT GATGGAGATG 
CGCCTCCGGG ATGCTCCGAA TCACTTCAAT GCAGCCCTGT TGCAGCTGGG CCTCAAGTAC 
CTCTTTATCC TTGGAGCTCA GATTCTAGCT TGTGCCTTGG CAGCTTCCAT CCTCCGCAGG 
CACCTCATGG TCTGGAAGGT GTTTGCTCCC AAGTTCATTT TTGAAGCCGT AGGTTTCATC 
GTGAGCAGTG TGGGACTTCT CCTGGGCATA GCATTGGTGA TGCGAGTGGA TGTCGCTGTG 
AGTTCCTGGT TCAAAAAACT AGTTCTGGCC CAGCAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pigo ( XM_011250078.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248380.1 Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X2, mRNA.

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CloneID	OMu33064
Clone ID Related Accession (Same CDS sequence)	XM_006538113.2 , XM_011250078.2 , XM_006538113.3 , XM_011250078.3 , NM_001305386.1
Accession Version	XM_011250078.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	GPI ethanolamine phosphate transferase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250078.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAAGG TGTCTGTGCT CCTCTTCTTA GCCTGGGTCT GTTTTCTTTT CTACGCTGGC 
ATTGCACTCT TCACCAGTGG CTTCCTTCTC ACTCGTCTGG AGCTCACCAA TCAAAGCAGC 
TGCCAAGAGC TCCCAGGCCC TGGTCCCTTG CCGTGGGGAA GTCATGGAAA GCCTGGGGCT 
TGCTGGATGC CTTCCCGCTT CTCCAGGGTG GTTTTGGTAC TGATAGACGC TCTGCGGTTT 
GACTTTGCCC AGCCTCAGCG CTCGCATGTG CCTGGGGAAC CTCCTGTCTC CGTGCCTTTT 
CTGGGAAAAT TAGGTTCCTT GCAGAGGATC CTAGAGAGTC AACCCCACCA TGGCCGGCTG 
TACCGATCTC AGGTGGACCC CCCCACGACC ACCATGCAGC GTCTAAAGGC TCTCACCACT 
GGCTCGCTGC CGACTTTCAT TGATGCTGGC AGTAACTTTG CCAGCCATGC TATAGTGGAA 
GATAATGTCA TCCAACAGCT CAACAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT 
ACCTGGAGAG ACCTCTTCCC CGGAGCCTTT TCTCAAGCTT TCTTCTTCTC ATCCTTCAAT 
GTCAGAGACC TGCACACAGT GGATAACGGC ATCTTGGAAC ACCTCTATCC TACCCTGGAC 
GGCGGTTCGT GGGATGTGCT GATCGCTCAC TTCCTGGGTG TGGATCACTG TGGTCACAAG 
CACGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATACAG 
GGACTCATAG AACGCCTGGA GAATGACACA CTGCTGGTGG TAGCCGGTGA CCATGGGATG 
ACCATGAATG GGGACCATGG AGGGGACAGT GAGCTGGAGG TGTCAGCTGC ACTTTTCCTG 
TACAGCCCCA CAGCTCTCTT CCCTAGTGTC CCACCCGAGG AGCCGGAGGT TATTCCTCAG 
GTCAGCCTTG TGCCCACGCT GGCACTGCTG CTAGGCCTGC CCATCCCGTT TGGGAACACT 
GGGGAAGTGA TGGCAGAGCT ATTCTCAGGT GGCAGTGACT CCTCCCATCC TCACTCCTCT 
GCGCTAGCCC AAGTCTCGGC TCTCCACATC AATGCCCAGC AGGTGTCCCG GTTTCTTCAT 
ACCTACTCGG CTGCGACTCA GGATCTCCAA GTTAAAGAAC TTCATCGGCT GCAGACGCTC 
TTCTCCAAGG CCTCTGCGCG TTACCAGCAC TTTCTGCGGG ATCCCCAAGA GGCTGAGGCA 
GCGCTGAGCA CATTGACTGC TGAGTTCCAG CAGTTCCTGC GGGGAGCTCG GGCCCTGTGC 
ATCGAGTCTT GGGCCCGCTT CTCTCTGGTC CGAATGGCAG GGGGCGCTGC TCTTTTGGCT 
GCTGCCTGCC TTCTCTGCCT GCTTGCTTCC CAGTTGGCAG TCGCCCCAGG CTTTCTCTTC 
CGCCCACTAC TGCTAATACC TGTAGCCTGG GGCCTAACTT GGACCATCCT GTATGCTGGA 
GTCTCGGTAA CTACTGGGTC GAAGATAGAC TTAGTGGTTC TGGGGGCTGT GGCTGCAGCC 
GGTTCACTCC TCCCTTTTCT GTGGAAAGCT TGGGTTAGCA GGGGGTCTAA GAGGCCACTG 
GCTCCCCTAC TTCCTGTCCC TAGACCTGTC CTAATATTAC TGCTCATTCG CTTGGCCACC 
TTCTTCTCGG ATAGCTTTGT TGTTGCTGAG GCCAGGGCCA CCCCCTTCCT TCTGGGCTCT 
CTCGTTTTCT TCCTGGTTGC TCAGCTCCAC TGGGAGGGTC AGCTACTGCC ACCCAAACCA 
CTCACGATGT CCCGCCTTGG CTCTTCTGCC CCAACAGCGC CCCCACGGCA CAGTGGCGCG 
CATGCCCTGT GGCTTGGAAT TGGGTTGCTT TTATTTACAA GGCTCGCTGG GCTTTTTCAC 
CGCTGCCCGG AAGAGACACC TGCCTGTCGC TCCTCTCCCT GGCTGAGTCC TCTGGCATCT 
ATGGTGGGAG GTCGAGCCAA GAATTTGTGG TACGGAGCTT GCGTGGGGGC ACTGGTGGCC 
CTGTTAGTCG TCGTGCGCTT GTGGCTTCGC CGCTATGGTA ATCTCAAGAG TCCTGAGCCC 
CCTGTGCTCT TTGTGCGCTG GGGGATGCCT CTCATGGTAC TGGGCACGGC TGCCTACTGG 
GCACTGGCAT CAGGTGCAGA GGAAGCACCA CCACGCCTCC GGGCCCTGGT CGCCGGGGCA 
TCAGCTGTGC TGCCTCGGGC TGTGATGGGG CTGGCTGCCT TGGGGCTCGT GCTGCTGCTC 
TGGAGGCCTG TGACAGTGCT GGTGAAAGCT GGGGCTGCTA CTTCAAGGAC CAGGACTATC 
CTCACTCCCT TCTCAGGTCC CCCTACTTCT CAGGCTGACC TCGACTATGT AGTCCCACAA 
ATCTACCGCC ACATGCAGGA GGAGTTCCAG GGCCGGCTCG AGAGGACTAA AGCTCAGGGT 
CCCATAACCG TGGCTGCCTA TCAGTTGGGA AGCGTGTACT CGGCTGCTAT GGTCACGGCC 
CTCCTCCTCT TGGCCTTCCC ACTTATGCTG TTGCATGTAG AGCGGGTCAG CCTGGTGTTC 
CTGCTCCTGT TTCTGCAGAG CTTCCTTCTC CTGCATCTGC TGGCCGCTGG GACACCAGTC 
GCCACCCCTG GCCCTTTTAC TGTGCTGTGG CAGGCAGTCT CAGCTTGGGT CCTTCTGGCT 
ACCCAGACCT TCTACTCCAC AGGCCACCAG CCTGTCTTTT CAGCCATCCA TTGGCACGCA 
GCCTTTGTGG GCTTCCCAGA CGGCCATGGC TCTTCTACCT GGTTGCCTGC CTTACTAGTG 
GGAGCTAACA CCTTTGCCTC CCACCTCCTC TTCGCAGTAG GCTGCCCACT GCTCCTGCTC 
TGGCCCTTCC TGTGTGAGCG TCAAGGGCCA AAGAGAAGGC AGCCCCTCCC GGGAAGTGAG 
TCTGAAGCCA GAGTCCGGCC TGAGGAAGAG GAGGAGCTGC AGGAGCCACT GATGGAGATG 
CGCCTCCGGG ATGCTCCGAA TCACTTCAAT GCAGCCCTGT TGCAGCTGGG CCTCAAGTAC 
CTCTTTATCC TTGGAGCTCA GATTCTAGCT TGTGCCTTGG CAGCTTCCAT CCTCCGCAGG 
CACCTCATGG TCTGGAAGGT GTTTGCTCCC AAGTTCATTT TTGAAGCCGT AGGTTTCATC 
GTGAGCAGTG TGGGACTTCT CCTGGGCATA GCATTGGTGA TGCGAGTGGA TGTCGCTGTG 
AGTTCCTGGT TCAAAAAACT AGTTCTGGCC CAGCAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248380.1
CDS	830..4111
Translation	MRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLASQLAVAPGFLFRPLLLIPVAWGLTWTILYAGVSVTTGSKIDLVVLGAVAAAGSLLPFLWKAWVSRGSKRPLAPLLPVPRPVLILLLIRLATFFSDSFVVAEARATPFLLGSLVFFLVAQLHWEGQLLPPKPLTMSRLGSSAPTAPPRHSGAHALWLGIGLLLFTRLAGLFHRCPEETPACRSSPWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPEPPVLFVRWGMPLMVLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWRPVTVLVKAGAATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSVYSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPFTVLWQAVSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEPLMEMRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR

Target ORF information:

RefSeq Version	XM_011250078.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class O (Pigo), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250078.2

ORF Insert Sequence:

ATGCGGAAGG TGTCTGTGCT CCTCTTCTTA GCCTGGGTCT GTTTTCTTTT CTACGCTGGC 
ATTGCACTCT TCACCAGTGG CTTCCTTCTC ACTCGTCTGG AGCTCACCAA TCAAAGCAGC 
TGCCAAGAGC TCCCAGGCCC TGGTCCCTTG CCGTGGGGAA GTCATGGAAA GCCTGGGGCT 
TGCTGGATGC CTTCCCGCTT CTCCAGGGTG GTTTTGGTAC TGATAGACGC TCTGCGGTTT 
GACTTTGCCC AGCCTCAGCG CTCGCATGTG CCTGGGGAAC CTCCTGTCTC CGTGCCTTTT 
CTGGGAAAAT TAGGTTCCTT GCAGAGGATC CTAGAGAGTC AACCCCACCA TGGCCGGCTG 
TACCGATCTC AGGTGGACCC CCCCACGACC ACCATGCAGC GTCTAAAGGC TCTCACCACT 
GGCTCGCTGC CGACTTTCAT TGATGCTGGC AGTAACTTTG CCAGCCATGC TATAGTGGAA 
GATAATGTCA TCCAACAGCT CAACAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT 
ACCTGGAGAG ACCTCTTCCC CGGAGCCTTT TCTCAAGCTT TCTTCTTCTC ATCCTTCAAT 
GTCAGAGACC TGCACACAGT GGATAACGGC ATCTTGGAAC ACCTCTATCC TACCCTGGAC 
GGCGGTTCGT GGGATGTGCT GATCGCTCAC TTCCTGGGTG TGGATCACTG TGGTCACAAG 
CACGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATACAG 
GGACTCATAG AACGCCTGGA GAATGACACA CTGCTGGTGG TAGCCGGTGA CCATGGGATG 
ACCATGAATG GGGACCATGG AGGGGACAGT GAGCTGGAGG TGTCAGCTGC ACTTTTCCTG 
TACAGCCCCA CAGCTCTCTT CCCTAGTGTC CCACCCGAGG AGCCGGAGGT TATTCCTCAG 
GTCAGCCTTG TGCCCACGCT GGCACTGCTG CTAGGCCTGC CCATCCCGTT TGGGAACACT 
GGGGAAGTGA TGGCAGAGCT ATTCTCAGGT GGCAGTGACT CCTCCCATCC TCACTCCTCT 
GCGCTAGCCC AAGTCTCGGC TCTCCACATC AATGCCCAGC AGGTGTCCCG GTTTCTTCAT 
ACCTACTCGG CTGCGACTCA GGATCTCCAA GTTAAAGAAC TTCATCGGCT GCAGACGCTC 
TTCTCCAAGG CCTCTGCGCG TTACCAGCAC TTTCTGCGGG ATCCCCAAGA GGCTGAGGCA 
GCGCTGAGCA CATTGACTGC TGAGTTCCAG CAGTTCCTGC GGGGAGCTCG GGCCCTGTGC 
ATCGAGTCTT GGGCCCGCTT CTCTCTGGTC CGAATGGCAG GGGGCGCTGC TCTTTTGGCT 
GCTGCCTGCC TTCTCTGCCT GCTTGCTTCC CAGTTGGCAG TCGCCCCAGG CTTTCTCTTC 
CGCCCACTAC TGCTAATACC TGTAGCCTGG GGCCTAACTT GGACCATCCT GTATGCTGGA 
GTCTCGGTAA CTACTGGGTC GAAGATAGAC TTAGTGGTTC TGGGGGCTGT GGCTGCAGCC 
GGTTCACTCC TCCCTTTTCT GTGGAAAGCT TGGGTTAGCA GGGGGTCTAA GAGGCCACTG 
GCTCCCCTAC TTCCTGTCCC TAGACCTGTC CTAATATTAC TGCTCATTCG CTTGGCCACC 
TTCTTCTCGG ATAGCTTTGT TGTTGCTGAG GCCAGGGCCA CCCCCTTCCT TCTGGGCTCT 
CTCGTTTTCT TCCTGGTTGC TCAGCTCCAC TGGGAGGGTC AGCTACTGCC ACCCAAACCA 
CTCACGATGT CCCGCCTTGG CTCTTCTGCC CCAACAGCGC CCCCACGGCA CAGTGGCGCG 
CATGCCCTGT GGCTTGGAAT TGGGTTGCTT TTATTTACAA GGCTCGCTGG GCTTTTTCAC 
CGCTGCCCGG AAGAGACACC TGCCTGTCGC TCCTCTCCCT GGCTGAGTCC TCTGGCATCT 
ATGGTGGGAG GTCGAGCCAA GAATTTGTGG TACGGAGCTT GCGTGGGGGC ACTGGTGGCC 
CTGTTAGTCG TCGTGCGCTT GTGGCTTCGC CGCTATGGTA ATCTCAAGAG TCCTGAGCCC 
CCTGTGCTCT TTGTGCGCTG GGGGATGCCT CTCATGGTAC TGGGCACGGC TGCCTACTGG 
GCACTGGCAT CAGGTGCAGA GGAAGCACCA CCACGCCTCC GGGCCCTGGT CGCCGGGGCA 
TCAGCTGTGC TGCCTCGGGC TGTGATGGGG CTGGCTGCCT TGGGGCTCGT GCTGCTGCTC 
TGGAGGCCTG TGACAGTGCT GGTGAAAGCT GGGGCTGCTA CTTCAAGGAC CAGGACTATC 
CTCACTCCCT TCTCAGGTCC CCCTACTTCT CAGGCTGACC TCGACTATGT AGTCCCACAA 
ATCTACCGCC ACATGCAGGA GGAGTTCCAG GGCCGGCTCG AGAGGACTAA AGCTCAGGGT 
CCCATAACCG TGGCTGCCTA TCAGTTGGGA AGCGTGTACT CGGCTGCTAT GGTCACGGCC 
CTCCTCCTCT TGGCCTTCCC ACTTATGCTG TTGCATGTAG AGCGGGTCAG CCTGGTGTTC 
CTGCTCCTGT TTCTGCAGAG CTTCCTTCTC CTGCATCTGC TGGCCGCTGG GACACCAGTC 
GCCACCCCTG GCCCTTTTAC TGTGCTGTGG CAGGCAGTCT CAGCTTGGGT CCTTCTGGCT 
ACCCAGACCT TCTACTCCAC AGGCCACCAG CCTGTCTTTT CAGCCATCCA TTGGCACGCA 
GCCTTTGTGG GCTTCCCAGA CGGCCATGGC TCTTCTACCT GGTTGCCTGC CTTACTAGTG 
GGAGCTAACA CCTTTGCCTC CCACCTCCTC TTCGCAGTAG GCTGCCCACT GCTCCTGCTC 
TGGCCCTTCC TGTGTGAGCG TCAAGGGCCA AAGAGAAGGC AGCCCCTCCC GGGAAGTGAG 
TCTGAAGCCA GAGTCCGGCC TGAGGAAGAG GAGGAGCTGC AGGAGCCACT GATGGAGATG 
CGCCTCCGGG ATGCTCCGAA TCACTTCAAT GCAGCCCTGT TGCAGCTGGG CCTCAAGTAC 
CTCTTTATCC TTGGAGCTCA GATTCTAGCT TGTGCCTTGG CAGCTTCCAT CCTCCGCAGG 
CACCTCATGG TCTGGAAGGT GTTTGCTCCC AAGTTCATTT TTGAAGCCGT AGGTTTCATC 
GTGAGCAGTG TGGGACTTCT CCTGGGCATA GCATTGGTGA TGCGAGTGGA TGTCGCTGTG 
AGTTCCTGGT TCAAAAAACT AGTTCTGGCC CAGCAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GPI7 is the second partner of PIG-F and involved in modification of glycosylphosphatidylinositol.
The Journal of biological chemistry280(10)9728-34(2005 Mar)
Shishioh N,Hong Y,Ohishi K,Ashida H,Maeda Y,Kinoshita T

Requirement of PIG-F and PIG-O for transferring phosphoethanolamine to the third mannose in glycosylphosphatidylinositol.
The Journal of biological chemistry275(27)20911-9(2000 Jul)
Hong Y,Maeda Y,Watanabe R,Inoue N,Ohishi K,Kinoshita T

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Pigo cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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