Poll cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Poll
Entrez Gene ID	56626
Full Name	polymerase (DNA directed), lambda
Synonyms	1110003P06Rik,AV007317
General protein information	Preferred Names polymerase (DNA directed), lambda Names DNA polymerase lambda pol Lambda
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 C3

mRNA and Protein(s)

mRNA	Protein	Name
XM_011247301.2	XP_011245603.1	DNA polymerase lambda isoform X1
NM_001330506.1	NP_001317435.1	DNA polymerase lambda isoform 1
NM_020032.3	NP_064416.1	DNA polymerase lambda isoform 1
NM_020032.2	NP_064416.1	DNA polymerase lambda
XM_006527232.1	XP_006527295.1	DNA polymerase lambda isoform X1
XM_011247301.1	XP_011245603.1	DNA polymerase lambda isoform X1
NM_001330507.1	NP_001317436.1	DNA polymerase lambda isoform 2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	poll	NP_998408.1
Pan troglodytes (chimpanzee)	POLL	XP_001170074.1
Mus musculus (house mouse)	Poll	NP_064416.1
Arabidopsis thaliana (thale cress)	AT1G10520	NP_172522.2
	POLL	XP_001110557.1
Xenopus tropicalis (tropical clawed frog)	poll	NP_001093716.1
Canis lupus familiaris (dog)	POLL	XP_850651.1
Bos taurus (cattle)	POLL	NP_001179488.1
Gallus gallus (chicken)	POLL	XP_001232209.3
Homo sapiens (human)	POLL	NP_037406.1
Rattus norvegicus (Norway rat)	Poll	NP_001014190.1
Oryza sativa (rice)	Os06g0237200	NP_001057247.1

Gene Ontology
Bibliography

Related articles in PubMed

Increased neuronal death and disturbed axonal growth in the Pol?-deficient mouse embryonic retina.
Baleriola J, ?lvarez-Lindo N, de la Villa P, Bernad A, Blanco L, Su?rez T, de la Rosa EJ
Scientific reports625928(2016 May)

Essential role for polymerase specialization in cellular nonhomologous end joining.
Pryor JM, Waters CA, Aza A, Asagoshi K, Strom C, Mieczkowski PA, Blanco L, Ramsden DA
Proceedings of the National Academy of Sciences of the United States of America112(33)E4537-45(2015 Aug)

DNA polymerases ? and ? and their roles in cell.
Belousova EA, Lavrik OI
DNA repair29112-26(2015 May)

Structure-function studies of DNA polymerase ?.
Bebenek K, Pedersen LC, Kunkel TA
Biochemistry53(17)2781-92(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pol mu and Pol lambda play a key role in conferring on NHEJ the flexibility required for accurate and efficient repair
Title: Essential role for polymerase specialization in cellular nonhomologous end joining.

Results show that deficiency of either DNA polymerases beta or lambda or both results in a modest but significant decrease in V region somatic hypermutation (SHM) with no effect on mutation specificity, suggesting no direct role in SHM.
Title: DNA polymerases ? and ? do not directly affect Ig variable region somatic hypermutation although their absence reduces the frequency of mutations.

both pol lambda and pol beta interact with the upstream DNA glycosylases for repair of alkylated and oxidized DNA bases
Title: DNA polymerases beta and lambda mediate overlapping and independent roles in base excision repair in mouse embryonic fibroblasts.

analysis of the interaction between DNA Polymerase lambda and anticancer nucleoside analogs
Title: Interaction between DNA Polymerase lambda and anticancer nucleoside analogs.

third hypervariable region assumes for each immunoglobulin chain, with pol lambda maintaining a large heavy chain junctional heterogeneity and pol mu ensuring a restricted light chain junctional variability
Title: Nonoverlapping functions of DNA polymerases mu, lambda, and terminal deoxynucleotidyltransferase during immunoglobulin V(D)J recombination in vivo.

The following Poll gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Poll cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu83476	NM_001330507.1 Latest version!	Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu13441	NM_020032.3 Latest version!	Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu13441	NM_001330506.1 Latest version!	Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu13441	XM_011247301.2 Latest version!	Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu13441	XM_006527232.1 Latest version!	Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
View Old Accession Versions >>
OMu13441	NM_020032.2	Mus musculus polymerase (DNA directed), lambda (Poll), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu13441	XM_011247301.1	Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Poll ( NM_001330507.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001317436.1 Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu83476
Clone ID Related Accession (Same CDS sequence)	NM_001330507.1
Accession Version	NM_001330507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	DNA polymerase lambda isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY315476.1, BY270661.1, BG080424.2, AK003392.1, AK133087.1 and BE949923.1. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK133087.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCTC AGGGCATCGT GAAGGCCTTT CCCAAGCGGA AGAAAAGTCA TGCAGATCTA 
TCTTCAAAAG CACTTGCAAA GATTCCCAAA AGGGAGGTGG GAGAAGCTAG AGGATGGCTG 
AGCTCCCTGA GAGCCCACAT TATGCCCGCT GGCATTGGAC GTGCCCGGGC TGAACTCTTT 
GAGAAGCAGA TTATCCACCA TGGCGGCCAG GTGTGCTCTG CCCAGGCCCC AGGAGTCACT 
CACATTGTGG TGGATGAAGA CATGGACTAT GAGCGGGCTC TCCGGCTCCT CAGGTTGCCC 
CAGCTACCCC CTGGTGCCCA GCTGGTGAAG TCCACCTGGC TGAGCTTGTG CCTGCAGGAG 
GGAAGGCTGA CAGACACGGA AGGATTCAGC CTTCCCATGC CCAAGAGGTC CTTGGATGAA 
CCACAGCCCA GCAAGTCAGG CCAAGATGCT TCTGCTCCTG GCACCCAGCG GGATTTACCC 
AGGACCACCC TTTCTCTTTC CCCTCCTCAC ACCAGAGCTG TATCTCCTCC CCCAACGGCA 
GAAAAGCCAT CAAGAACCCA AGCCCAGCTC AGCTCAGAGG ATGAAACCAG CGATGGAGAA 
GGGCCCCAGG TTAGCTCAGC AGATCTGCAA GCCTTGATCA CTGGGCACTA CCCCACTCCC 
CCTGAGGAAG ATGGTGGGCC TGACCCAGCA CCAGAAGCTC TGGATAAATG GGTCTGTGCA 
CAGCCCTCGA GCCAGAAGGC AACTAACTAC AATCTGCACA TCACAGAGAA GCTGGAAGTG 
CTGGCTAAAG CCTACAGTGT CCAGGGAGAC AAGTGGAGGG CCCTGGGCTA TGCAAAGGCC 
ATCAATGCTC TCAAGAGCTT CCACAAGCCT GTCAGCTCCT ACCAGGAGGC CTGTAGCATC 
CCGGGAATTG GCAAGCGGAT GGCGGAGAAG GTCATGGAGA TCCTGGAGAG TGGGCATCTG 
CGGAAGCTAG ACCACATCAG TGACAGTGTG CCTGTCTTGG AGCTCTTCTC CAACATCTGG 
GGAGCTGGGA CGAAGACTGC CCAGATGTGG TACCATCAGG GCTTCCGAAA CCTAGAAGAT 
CTCCAAAGCC TGGGCTCCCT GACCGCTCAG CAGGCCATTG GCTTGAAGCA CTACGATGAC 
TTCCTGGACC GCATGCCCAG GGAGGAGGCT GCGGAAATTG AGCAGACGGT CCGGATATCA 
GCCCAGGCCT TCAACCCTGG GCTGCTGTGT GTGGCTTGTG GCTCTTACCG ACGGGGGAAG 
ATGACGTGCG GGGATGTGGA TGTACTCATT ACTCACCCCG ATGGCCGATC CCACCGGGGC 
ATCTTCAGCT GCCTCCTTGA TAGCCTTCGG CAGCAAGATG AGGAATTGGG CCCAGAGGGG 
CGAGGCCACT CAACTCAACA CACAAATAGG CACAGGGTAT GCTGGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317436.1
CDS	105..1535
Translation	MDPQGIVKAFPKRKKSHADLSSKALAKIPKREVGEARGWLSSLRAHIMPAGIGRARAELFEKQIIHHGGQVCSAQAPGVTHIVVDEDMDYERALRLLRLPQLPPGAQLVKSTWLSLCLQEGRLTDTEGFSLPMPKRSLDEPQPSKSGQDASAPGTQRDLPRTTLSLSPPHTRAVSPPPTAEKPSRTQAQLSSEDETSDGEGPQVSSADLQALITGHYPTPPEEDGGPDPAPEALDKWVCAQPSSQKATNYNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVSSYQEACSIPGIGKRMAEKVMEILESGHLRKLDHISDSVPVLELFSNIWGAGTKTAQMWYHQGFRNLEDLQSLGSLTAQQAIGLKHYDDFLDRMPREEAAEIEQTVRISAQAFNPGLLCVACGSYRRGKMTCGDVDVLITHPDGRSHRGIFSCLLDSLRQQDEELGPEGRGHSTQHTNRHRVCWE

Target ORF information:

RefSeq Version	NM_001330507.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330507.1

ORF Insert Sequence:

ATGGACCCTC AGGGCATCGT GAAGGCCTTT CCCAAGCGGA AGAAAAGTCA TGCAGATCTA 
TCTTCAAAAG CACTTGCAAA GATTCCCAAA AGGGAGGTGG GAGAAGCTAG AGGATGGCTG 
AGCTCCCTGA GAGCCCACAT TATGCCCGCT GGCATTGGAC GTGCCCGGGC TGAACTCTTT 
GAGAAGCAGA TTATCCACCA TGGCGGCCAG GTGTGCTCTG CCCAGGCCCC AGGAGTCACT 
CACATTGTGG TGGATGAAGA CATGGACTAT GAGCGGGCTC TCCGGCTCCT CAGGTTGCCC 
CAGCTACCCC CTGGTGCCCA GCTGGTGAAG TCCACCTGGC TGAGCTTGTG CCTGCAGGAG 
GGAAGGCTGA CAGACACGGA AGGATTCAGC CTTCCCATGC CCAAGAGGTC CTTGGATGAA 
CCACAGCCCA GCAAGTCAGG CCAAGATGCT TCTGCTCCTG GCACCCAGCG GGATTTACCC 
AGGACCACCC TTTCTCTTTC CCCTCCTCAC ACCAGAGCTG TATCTCCTCC CCCAACGGCA 
GAAAAGCCAT CAAGAACCCA AGCCCAGCTC AGCTCAGAGG ATGAAACCAG CGATGGAGAA 
GGGCCCCAGG TTAGCTCAGC AGATCTGCAA GCCTTGATCA CTGGGCACTA CCCCACTCCC 
CCTGAGGAAG ATGGTGGGCC TGACCCAGCA CCAGAAGCTC TGGATAAATG GGTCTGTGCA 
CAGCCCTCGA GCCAGAAGGC AACTAACTAC AATCTGCACA TCACAGAGAA GCTGGAAGTG 
CTGGCTAAAG CCTACAGTGT CCAGGGAGAC AAGTGGAGGG CCCTGGGCTA TGCAAAGGCC 
ATCAATGCTC TCAAGAGCTT CCACAAGCCT GTCAGCTCCT ACCAGGAGGC CTGTAGCATC 
CCGGGAATTG GCAAGCGGAT GGCGGAGAAG GTCATGGAGA TCCTGGAGAG TGGGCATCTG 
CGGAAGCTAG ACCACATCAG TGACAGTGTG CCTGTCTTGG AGCTCTTCTC CAACATCTGG 
GGAGCTGGGA CGAAGACTGC CCAGATGTGG TACCATCAGG GCTTCCGAAA CCTAGAAGAT 
CTCCAAAGCC TGGGCTCCCT GACCGCTCAG CAGGCCATTG GCTTGAAGCA CTACGATGAC 
TTCCTGGACC GCATGCCCAG GGAGGAGGCT GCGGAAATTG AGCAGACGGT CCGGATATCA 
GCCCAGGCCT TCAACCCTGG GCTGCTGTGT GTGGCTTGTG GCTCTTACCG ACGGGGGAAG 
ATGACGTGCG GGGATGTGGA TGTACTCATT ACTCACCCCG ATGGCCGATC CCACCGGGGC 
ATCTTCAGCT GCCTCCTTGA TAGCCTTCGG CAGCAAGATG AGGAATTGGG CCCAGAGGGG 
CGAGGCCACT CAACTCAACA CACAAATAGG CACAGGGTAT GCTGGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Poll ( NM_001330506.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001317435.1 Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13441
Clone ID Related Accession (Same CDS sequence)	XM_011247301.2 , NM_001330506.1 , NM_020032.3 , NM_020032.2 , XM_006527232.1 , XM_011247301.1
Accession Version	NM_001330506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	DNA polymerase lambda isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126454.3, BY270661.1, BG080424.2, AK003392.1 and BE949923.1. On Aug 26, 2016 this sequence version replaced XM_006527232.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.47980.1, SRR1660815.227768.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCTC AGGGCATCGT GAAGGCCTTT CCCAAGCGGA AGAAAAGTCA TGCAGATCTA 
TCTTCAAAAG CACTTGCAAA GATTCCCAAA AGGGAGGTGG GAGAAGCTAG AGGATGGCTG 
AGCTCCCTGA GAGCCCACAT TATGCCCGCT GGCATTGGAC GTGCCCGGGC TGAACTCTTT 
GAGAAGCAGA TTATCCACCA TGGCGGCCAG GTGTGCTCTG CCCAGGCCCC AGGAGTCACT 
CACATTGTGG TGGATGAAGA CATGGACTAT GAGCGGGCTC TCCGGCTCCT CAGGTTGCCC 
CAGCTACCCC CTGGTGCCCA GCTGGTGAAG TCCACCTGGC TGAGCTTGTG CCTGCAGGAG 
GGAAGGCTGA CAGACACGGA AGGATTCAGC CTTCCCATGC CCAAGAGGTC CTTGGATGAA 
CCACAGCCCA GCAAGTCAGG CCAAGATGCT TCTGCTCCTG GCACCCAGCG GGATTTACCC 
AGGACCACCC TTTCTCTTTC CCCTCCTCAC ACCAGAGCTG TATCTCCTCC CCCAACGGCA 
GAAAAGCCAT CAAGAACCCA AGCCCAGCTC AGCTCAGAGG ATGAAACCAG CGATGGAGAA 
GGGCCCCAGG TTAGCTCAGC AGATCTGCAA GCCTTGATCA CTGGGCACTA CCCCACTCCC 
CCTGAGGAAG ATGGTGGGCC TGACCCAGCA CCAGAAGCTC TGGATAAATG GGTCTGTGCA 
CAGCCCTCGA GCCAGAAGGC AACTAACTAC AATCTGCACA TCACAGAGAA GCTGGAAGTG 
CTGGCTAAAG CCTACAGTGT CCAGGGAGAC AAGTGGAGGG CCCTGGGCTA TGCAAAGGCC 
ATCAATGCTC TCAAGAGCTT CCACAAGCCT GTCAGCTCCT ACCAGGAGGC CTGTAGCATC 
CCGGGAATTG GCAAGCGGAT GGCGGAGAAG GTCATGGAGA TCCTGGAGAG TGGGCATCTG 
CGGAAGCTAG ACCACATCAG TGACAGTGTG CCTGTCTTGG AGCTCTTCTC CAACATCTGG 
GGAGCTGGGA CGAAGACTGC CCAGATGTGG TACCATCAGG GCTTCCGAAA CCTAGAAGAT 
CTCCAAAGCC TGGGCTCCCT GACCGCTCAG CAGGCCATTG GCTTGAAGCA CTACGATGAC 
TTCCTGGACC GCATGCCCAG GGAGGAGGCT GCGGAAATTG AGCAGACGGT CCGGATATCA 
GCCCAGGCCT TCAACCCTGG GCTGCTGTGT GTGGCTTGTG GCTCTTACCG ACGGGGGAAG 
ATGACGTGCG GGGATGTGGA TGTACTCATT ACTCACCCCG ATGGCCGATC CCACCGGGGC 
ATCTTCAGCT GCCTCCTTGA TAGCCTTCGG CAGCAAGGGT TCCTCACAGA TGACTTGGTG 
AGCCAGGAGG AGAACGGCCA ACAGCAGAAA TACCTGGGTG TGTGCCGGCT CCCAGGGCCC 
GGGAAGCGCC ACCGGCGACT GGACATCATC GTGGTACCCT ACTGTGAGTT TGCCTGTGCC 
CTGCTCTACT TCACCGGCTC TGCCCACTTC AACCGGTCCA TGAGAGCTCT GGCCAAGACC 
AAGGGCATGA GCCTGTCGGA GCATGCGCTC AGTGCAGCTG TAGTCCGGAA CAGCCAAGGT 
GTCAAGGTGG GGCCTGGACA AGTGCTGCCC ACCCCCACGG AGAAGGACGT CTTCAAGCAC 
TTAGGCCTGC CCTACCGGGA GCCAGCTGAA CGGGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317435.1
CDS	273..1994
Translation	MDPQGIVKAFPKRKKSHADLSSKALAKIPKREVGEARGWLSSLRAHIMPAGIGRARAELFEKQIIHHGGQVCSAQAPGVTHIVVDEDMDYERALRLLRLPQLPPGAQLVKSTWLSLCLQEGRLTDTEGFSLPMPKRSLDEPQPSKSGQDASAPGTQRDLPRTTLSLSPPHTRAVSPPPTAEKPSRTQAQLSSEDETSDGEGPQVSSADLQALITGHYPTPPEEDGGPDPAPEALDKWVCAQPSSQKATNYNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVSSYQEACSIPGIGKRMAEKVMEILESGHLRKLDHISDSVPVLELFSNIWGAGTKTAQMWYHQGFRNLEDLQSLGSLTAQQAIGLKHYDDFLDRMPREEAAEIEQTVRISAQAFNPGLLCVACGSYRRGKMTCGDVDVLITHPDGRSHRGIFSCLLDSLRQQGFLTDDLVSQEENGQQQKYLGVCRLPGPGKRHRRLDIIVVPYCEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSAAVVRNSQGVKVGPGQVLPTPTEKDVFKHLGLPYREPAERDW

Target ORF information:

RefSeq Version	NM_001330506.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330506.1

ORF Insert Sequence:

ATGGACCCTC AGGGCATCGT GAAGGCCTTT CCCAAGCGGA AGAAAAGTCA TGCAGATCTA 
TCTTCAAAAG CACTTGCAAA GATTCCCAAA AGGGAGGTGG GAGAAGCTAG AGGATGGCTG 
AGCTCCCTGA GAGCCCACAT TATGCCCGCT GGCATTGGAC GTGCCCGGGC TGAACTCTTT 
GAGAAGCAGA TTATCCACCA TGGCGGCCAG GTGTGCTCTG CCCAGGCCCC AGGAGTCACT 
CACATTGTGG TGGATGAAGA CATGGACTAT GAGCGGGCTC TCCGGCTCCT CAGGTTGCCC 
CAGCTACCCC CTGGTGCCCA GCTGGTGAAG TCCACCTGGC TGAGCTTGTG CCTGCAGGAG 
GGAAGGCTGA CAGACACGGA AGGATTCAGC CTTCCCATGC CCAAGAGGTC CTTGGATGAA 
CCACAGCCCA GCAAGTCAGG CCAAGATGCT TCTGCTCCTG GCACCCAGCG GGATTTACCC 
AGGACCACCC TTTCTCTTTC CCCTCCTCAC ACCAGAGCTG TATCTCCTCC CCCAACGGCA 
GAAAAGCCAT CAAGAACCCA AGCCCAGCTC AGCTCAGAGG ATGAAACCAG CGATGGAGAA 
GGGCCCCAGG TTAGCTCAGC AGATCTGCAA GCCTTGATCA CTGGGCACTA CCCCACTCCC 
CCTGAGGAAG ATGGTGGGCC TGACCCAGCA CCAGAAGCTC TGGATAAATG GGTCTGTGCA 
CAGCCCTCGA GCCAGAAGGC AACTAACTAC AATCTGCACA TCACAGAGAA GCTGGAAGTG 
CTGGCTAAAG CCTACAGTGT CCAGGGAGAC AAGTGGAGGG CCCTGGGCTA TGCAAAGGCC 
ATCAATGCTC TCAAGAGCTT CCACAAGCCT GTCAGCTCCT ACCAGGAGGC CTGTAGCATC 
CCGGGAATTG GCAAGCGGAT GGCGGAGAAG GTCATGGAGA TCCTGGAGAG TGGGCATCTG 
CGGAAGCTAG ACCACATCAG TGACAGTGTG CCTGTCTTGG AGCTCTTCTC CAACATCTGG 
GGAGCTGGGA CGAAGACTGC CCAGATGTGG TACCATCAGG GCTTCCGAAA CCTAGAAGAT 
CTCCAAAGCC TGGGCTCCCT GACCGCTCAG CAGGCCATTG GCTTGAAGCA CTACGATGAC 
TTCCTGGACC GCATGCCCAG GGAGGAGGCT GCGGAAATTG AGCAGACGGT CCGGATATCA 
GCCCAGGCCT TCAACCCTGG GCTGCTGTGT GTGGCTTGTG GCTCTTACCG ACGGGGGAAG 
ATGACGTGCG GGGATGTGGA TGTACTCATT ACTCACCCCG ATGGCCGATC CCACCGGGGC 
ATCTTCAGCT GCCTCCTTGA TAGCCTTCGG CAGCAAGGGT TCCTCACAGA TGACTTGGTG 
AGCCAGGAGG AGAACGGCCA ACAGCAGAAA TACCTGGGTG TGTGCCGGCT CCCAGGGCCC 
GGGAAGCGCC ACCGGCGACT GGACATCATC GTGGTACCCT ACTGTGAGTT TGCCTGTGCC 
CTGCTCTACT TCACCGGCTC TGCCCACTTC AACCGGTCCA TGAGAGCTCT GGCCAAGACC 
AAGGGCATGA GCCTGTCGGA GCATGCGCTC AGTGCAGCTG TAGTCCGGAA CAGCCAAGGT 
GTCAAGGTGG GGCCTGGACA AGTGCTGCCC ACCCCCACGG AGAAGGACGT CTTCAAGCAC 
TTAGGCCTGC CCTACCGGGA GCCAGCTGAA CGGGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Poll ( NM_020032.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064416.1 Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13441
Clone ID Related Accession (Same CDS sequence)	XM_011247301.2 , NM_001330506.1 , NM_020032.3 , NM_020032.2 , XM_006527232.1 , XM_011247301.1
Accession Version	NM_020032.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	DNA polymerase lambda isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY315476.1, BY270661.1, BG080424.2, AK003392.1 and BE949923.1. On Aug 26, 2016 this sequence version replaced NM_020032.2. Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (1). Both variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ131889.1, SRR1660813.45415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCTC AGGGCATCGT GAAGGCCTTT CCCAAGCGGA AGAAAAGTCA TGCAGATCTA 
TCTTCAAAAG CACTTGCAAA GATTCCCAAA AGGGAGGTGG GAGAAGCTAG AGGATGGCTG 
AGCTCCCTGA GAGCCCACAT TATGCCCGCT GGCATTGGAC GTGCCCGGGC TGAACTCTTT 
GAGAAGCAGA TTATCCACCA TGGCGGCCAG GTGTGCTCTG CCCAGGCCCC AGGAGTCACT 
CACATTGTGG TGGATGAAGA CATGGACTAT GAGCGGGCTC TCCGGCTCCT CAGGTTGCCC 
CAGCTACCCC CTGGTGCCCA GCTGGTGAAG TCCACCTGGC TGAGCTTGTG CCTGCAGGAG 
GGAAGGCTGA CAGACACGGA AGGATTCAGC CTTCCCATGC CCAAGAGGTC CTTGGATGAA 
CCACAGCCCA GCAAGTCAGG CCAAGATGCT TCTGCTCCTG GCACCCAGCG GGATTTACCC 
AGGACCACCC TTTCTCTTTC CCCTCCTCAC ACCAGAGCTG TATCTCCTCC CCCAACGGCA 
GAAAAGCCAT CAAGAACCCA AGCCCAGCTC AGCTCAGAGG ATGAAACCAG CGATGGAGAA 
GGGCCCCAGG TTAGCTCAGC AGATCTGCAA GCCTTGATCA CTGGGCACTA CCCCACTCCC 
CCTGAGGAAG ATGGTGGGCC TGACCCAGCA CCAGAAGCTC TGGATAAATG GGTCTGTGCA 
CAGCCCTCGA GCCAGAAGGC AACTAACTAC AATCTGCACA TCACAGAGAA GCTGGAAGTG 
CTGGCTAAAG CCTACAGTGT CCAGGGAGAC AAGTGGAGGG CCCTGGGCTA TGCAAAGGCC 
ATCAATGCTC TCAAGAGCTT CCACAAGCCT GTCAGCTCCT ACCAGGAGGC CTGTAGCATC 
CCGGGAATTG GCAAGCGGAT GGCGGAGAAG GTCATGGAGA TCCTGGAGAG TGGGCATCTG 
CGGAAGCTAG ACCACATCAG TGACAGTGTG CCTGTCTTGG AGCTCTTCTC CAACATCTGG 
GGAGCTGGGA CGAAGACTGC CCAGATGTGG TACCATCAGG GCTTCCGAAA CCTAGAAGAT 
CTCCAAAGCC TGGGCTCCCT GACCGCTCAG CAGGCCATTG GCTTGAAGCA CTACGATGAC 
TTCCTGGACC GCATGCCCAG GGAGGAGGCT GCGGAAATTG AGCAGACGGT CCGGATATCA 
GCCCAGGCCT TCAACCCTGG GCTGCTGTGT GTGGCTTGTG GCTCTTACCG ACGGGGGAAG 
ATGACGTGCG GGGATGTGGA TGTACTCATT ACTCACCCCG ATGGCCGATC CCACCGGGGC 
ATCTTCAGCT GCCTCCTTGA TAGCCTTCGG CAGCAAGGGT TCCTCACAGA TGACTTGGTG 
AGCCAGGAGG AGAACGGCCA ACAGCAGAAA TACCTGGGTG TGTGCCGGCT CCCAGGGCCC 
GGGAAGCGCC ACCGGCGACT GGACATCATC GTGGTACCCT ACTGTGAGTT TGCCTGTGCC 
CTGCTCTACT TCACCGGCTC TGCCCACTTC AACCGGTCCA TGAGAGCTCT GGCCAAGACC 
AAGGGCATGA GCCTGTCGGA GCATGCGCTC AGTGCAGCTG TAGTCCGGAA CAGCCAAGGT 
GTCAAGGTGG GGCCTGGACA AGTGCTGCCC ACCCCCACGG AGAAGGACGT CTTCAAGCAC 
TTAGGCCTGC CCTACCGGGA GCCAGCTGAA CGGGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064416.1
CDS	105..1826
Translation	MDPQGIVKAFPKRKKSHADLSSKALAKIPKREVGEARGWLSSLRAHIMPAGIGRARAELFEKQIIHHGGQVCSAQAPGVTHIVVDEDMDYERALRLLRLPQLPPGAQLVKSTWLSLCLQEGRLTDTEGFSLPMPKRSLDEPQPSKSGQDASAPGTQRDLPRTTLSLSPPHTRAVSPPPTAEKPSRTQAQLSSEDETSDGEGPQVSSADLQALITGHYPTPPEEDGGPDPAPEALDKWVCAQPSSQKATNYNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVSSYQEACSIPGIGKRMAEKVMEILESGHLRKLDHISDSVPVLELFSNIWGAGTKTAQMWYHQGFRNLEDLQSLGSLTAQQAIGLKHYDDFLDRMPREEAAEIEQTVRISAQAFNPGLLCVACGSYRRGKMTCGDVDVLITHPDGRSHRGIFSCLLDSLRQQGFLTDDLVSQEENGQQQKYLGVCRLPGPGKRHRRLDIIVVPYCEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSAAVVRNSQGVKVGPGQVLPTPTEKDVFKHLGLPYREPAERDW

Target ORF information:

RefSeq Version	NM_020032.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020032.3

ORF Insert Sequence:

ATGGACCCTC AGGGCATCGT GAAGGCCTTT CCCAAGCGGA AGAAAAGTCA TGCAGATCTA 
TCTTCAAAAG CACTTGCAAA GATTCCCAAA AGGGAGGTGG GAGAAGCTAG AGGATGGCTG 
AGCTCCCTGA GAGCCCACAT TATGCCCGCT GGCATTGGAC GTGCCCGGGC TGAACTCTTT 
GAGAAGCAGA TTATCCACCA TGGCGGCCAG GTGTGCTCTG CCCAGGCCCC AGGAGTCACT 
CACATTGTGG TGGATGAAGA CATGGACTAT GAGCGGGCTC TCCGGCTCCT CAGGTTGCCC 
CAGCTACCCC CTGGTGCCCA GCTGGTGAAG TCCACCTGGC TGAGCTTGTG CCTGCAGGAG 
GGAAGGCTGA CAGACACGGA AGGATTCAGC CTTCCCATGC CCAAGAGGTC CTTGGATGAA 
CCACAGCCCA GCAAGTCAGG CCAAGATGCT TCTGCTCCTG GCACCCAGCG GGATTTACCC 
AGGACCACCC TTTCTCTTTC CCCTCCTCAC ACCAGAGCTG TATCTCCTCC CCCAACGGCA 
GAAAAGCCAT CAAGAACCCA AGCCCAGCTC AGCTCAGAGG ATGAAACCAG CGATGGAGAA 
GGGCCCCAGG TTAGCTCAGC AGATCTGCAA GCCTTGATCA CTGGGCACTA CCCCACTCCC 
CCTGAGGAAG ATGGTGGGCC TGACCCAGCA CCAGAAGCTC TGGATAAATG GGTCTGTGCA 
CAGCCCTCGA GCCAGAAGGC AACTAACTAC AATCTGCACA TCACAGAGAA GCTGGAAGTG 
CTGGCTAAAG CCTACAGTGT CCAGGGAGAC AAGTGGAGGG CCCTGGGCTA TGCAAAGGCC 
ATCAATGCTC TCAAGAGCTT CCACAAGCCT GTCAGCTCCT ACCAGGAGGC CTGTAGCATC 
CCGGGAATTG GCAAGCGGAT GGCGGAGAAG GTCATGGAGA TCCTGGAGAG TGGGCATCTG 
CGGAAGCTAG ACCACATCAG TGACAGTGTG CCTGTCTTGG AGCTCTTCTC CAACATCTGG 
GGAGCTGGGA CGAAGACTGC CCAGATGTGG TACCATCAGG GCTTCCGAAA CCTAGAAGAT 
CTCCAAAGCC TGGGCTCCCT GACCGCTCAG CAGGCCATTG GCTTGAAGCA CTACGATGAC 
TTCCTGGACC GCATGCCCAG GGAGGAGGCT GCGGAAATTG AGCAGACGGT CCGGATATCA 
GCCCAGGCCT TCAACCCTGG GCTGCTGTGT GTGGCTTGTG GCTCTTACCG ACGGGGGAAG 
ATGACGTGCG GGGATGTGGA TGTACTCATT ACTCACCCCG ATGGCCGATC CCACCGGGGC 
ATCTTCAGCT GCCTCCTTGA TAGCCTTCGG CAGCAAGGGT TCCTCACAGA TGACTTGGTG 
AGCCAGGAGG AGAACGGCCA ACAGCAGAAA TACCTGGGTG TGTGCCGGCT CCCAGGGCCC 
GGGAAGCGCC ACCGGCGACT GGACATCATC GTGGTACCCT ACTGTGAGTT TGCCTGTGCC 
CTGCTCTACT TCACCGGCTC TGCCCACTTC AACCGGTCCA TGAGAGCTCT GGCCAAGACC 
AAGGGCATGA GCCTGTCGGA GCATGCGCTC AGTGCAGCTG TAGTCCGGAA CAGCCAAGGT 
GTCAAGGTGG GGCCTGGACA AGTGCTGCCC ACCCCCACGG AGAAGGACGT CTTCAAGCAC 
TTAGGCCTGC CCTACCGGGA GCCAGCTGAA CGGGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Poll ( NM_020032.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064416.1 Mus musculus polymerase (DNA directed), lambda (Poll), mRNA.

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CloneID	OMu13441
Clone ID Related Accession (Same CDS sequence)	XM_011247301.2 , NM_001330506.1 , NM_020032.3 , NM_020032.2 , XM_006527232.1 , XM_011247301.1
Accession Version	NM_020032.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	DNA polymerase lambda
Comment

ATGGACCCTC AGGGCATCGT GAAGGCCTTT CCCAAGCGGA AGAAAAGTCA TGCAGATCTA 
TCTTCAAAAG CACTTGCAAA GATTCCCAAA AGGGAGGTGG GAGAAGCTAG AGGATGGCTG 
AGCTCCCTGA GAGCCCACAT TATGCCCGCT GGCATTGGAC GTGCCCGGGC TGAACTCTTT 
GAGAAGCAGA TTATCCACCA TGGCGGCCAG GTGTGCTCTG CCCAGGCCCC AGGAGTCACT 
CACATTGTGG TGGATGAAGA CATGGACTAT GAGCGGGCTC TCCGGCTCCT CAGGTTGCCC 
CAGCTACCCC CTGGTGCCCA GCTGGTGAAG TCCACCTGGC TGAGCTTGTG CCTGCAGGAG 
GGAAGGCTGA CAGACACGGA AGGATTCAGC CTTCCCATGC CCAAGAGGTC CTTGGATGAA 
CCACAGCCCA GCAAGTCAGG CCAAGATGCT TCTGCTCCTG GCACCCAGCG GGATTTACCC 
AGGACCACCC TTTCTCTTTC CCCTCCTCAC ACCAGAGCTG TATCTCCTCC CCCAACGGCA 
GAAAAGCCAT CAAGAACCCA AGCCCAGCTC AGCTCAGAGG ATGAAACCAG CGATGGAGAA 
GGGCCCCAGG TTAGCTCAGC AGATCTGCAA GCCTTGATCA CTGGGCACTA CCCCACTCCC 
CCTGAGGAAG ATGGTGGGCC TGACCCAGCA CCAGAAGCTC TGGATAAATG GGTCTGTGCA 
CAGCCCTCGA GCCAGAAGGC AACTAACTAC AATCTGCACA TCACAGAGAA GCTGGAAGTG 
CTGGCTAAAG CCTACAGTGT CCAGGGAGAC AAGTGGAGGG CCCTGGGCTA TGCAAAGGCC 
ATCAATGCTC TCAAGAGCTT CCACAAGCCT GTCAGCTCCT ACCAGGAGGC CTGTAGCATC 
CCGGGAATTG GCAAGCGGAT GGCGGAGAAG GTCATGGAGA TCCTGGAGAG TGGGCATCTG 
CGGAAGCTAG ACCACATCAG TGACAGTGTG CCTGTCTTGG AGCTCTTCTC CAACATCTGG 
GGAGCTGGGA CGAAGACTGC CCAGATGTGG TACCATCAGG GCTTCCGAAA CCTAGAAGAT 
CTCCAAAGCC TGGGCTCCCT GACCGCTCAG CAGGCCATTG GCTTGAAGCA CTACGATGAC 
TTCCTGGACC GCATGCCCAG GGAGGAGGCT GCGGAAATTG AGCAGACGGT CCGGATATCA 
GCCCAGGCCT TCAACCCTGG GCTGCTGTGT GTGGCTTGTG GCTCTTACCG ACGGGGGAAG 
ATGACGTGCG GGGATGTGGA TGTACTCATT ACTCACCCCG ATGGCCGATC CCACCGGGGC 
ATCTTCAGCT GCCTCCTTGA TAGCCTTCGG CAGCAAGGGT TCCTCACAGA TGACTTGGTG 
AGCCAGGAGG AGAACGGCCA ACAGCAGAAA TACCTGGGTG TGTGCCGGCT CCCAGGGCCC 
GGGAAGCGCC ACCGGCGACT GGACATCATC GTGGTACCCT ACTGTGAGTT TGCCTGTGCC 
CTGCTCTACT TCACCGGCTC TGCCCACTTC AACCGGTCCA TGAGAGCTCT GGCCAAGACC 
AAGGGCATGA GCCTGTCGGA GCATGCGCTC AGTGCAGCTG TAGTCCGGAA CAGCCAAGGT 
GTCAAGGTGG GGCCTGGACA AGTGCTGCCC ACCCCCACGG AGAAGGACGT CTTCAAGCAC 
TTAGGCCTGC CCTACCGGGA GCCAGCTGAA CGGGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_064416.1

Translation

MDPQGIVKAFPKRKKSHADLSSKALAKIPKREVGEARGWLSSLRAHIMPAGIGRARAELFEKQIIHHGGQVCSAQAPGVTHIVVDEDMDYERALRLLRLPQLPPGAQLVKSTWLSLCLQEGRLTDTEGFSLPMPKRSLDEPQPSKSGQDASAPGTQRDLPRTTLSLSPPHTRAVSPPPTAEKPSRTQAQLSSEDETSDGEGPQVSSADLQALITGHYPTPPEEDGGPDPAPEALDKWVCAQPSSQKATNYNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVSSYQEACSIPGIGKRMAEKVMEILESGHLRKLDHISDSVPVLELFSNIWGAGTKTAQMWYHQGFRNLEDLQSLGSLTAQQAIGLKHYDDFLDRMPREEAAEIEQTVRISAQAFNPGLLCVACGSYRRGKMTCGDVDVLITHPDGRSHRGIFSCLLDSLRQQGFLTDDLVSQEENGQQQKYLGVCRLPGPGKRHRRLDIIVVPYCEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSAAVVRNSQGVKVGPGQVLPTPTEKDVFKHLGLPYREPAERDW

Target ORF information:

RefSeq Version	NM_020032.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus polymerase (DNA directed), lambda (Poll), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020032.2

ORF Insert Sequence:

ATGGACCCTC AGGGCATCGT GAAGGCCTTT CCCAAGCGGA AGAAAAGTCA TGCAGATCTA 
TCTTCAAAAG CACTTGCAAA GATTCCCAAA AGGGAGGTGG GAGAAGCTAG AGGATGGCTG 
AGCTCCCTGA GAGCCCACAT TATGCCCGCT GGCATTGGAC GTGCCCGGGC TGAACTCTTT 
GAGAAGCAGA TTATCCACCA TGGCGGCCAG GTGTGCTCTG CCCAGGCCCC AGGAGTCACT 
CACATTGTGG TGGATGAAGA CATGGACTAT GAGCGGGCTC TCCGGCTCCT CAGGTTGCCC 
CAGCTACCCC CTGGTGCCCA GCTGGTGAAG TCCACCTGGC TGAGCTTGTG CCTGCAGGAG 
GGAAGGCTGA CAGACACGGA AGGATTCAGC CTTCCCATGC CCAAGAGGTC CTTGGATGAA 
CCACAGCCCA GCAAGTCAGG CCAAGATGCT TCTGCTCCTG GCACCCAGCG GGATTTACCC 
AGGACCACCC TTTCTCTTTC CCCTCCTCAC ACCAGAGCTG TATCTCCTCC CCCAACGGCA 
GAAAAGCCAT CAAGAACCCA AGCCCAGCTC AGCTCAGAGG ATGAAACCAG CGATGGAGAA 
GGGCCCCAGG TTAGCTCAGC AGATCTGCAA GCCTTGATCA CTGGGCACTA CCCCACTCCC 
CCTGAGGAAG ATGGTGGGCC TGACCCAGCA CCAGAAGCTC TGGATAAATG GGTCTGTGCA 
CAGCCCTCGA GCCAGAAGGC AACTAACTAC AATCTGCACA TCACAGAGAA GCTGGAAGTG 
CTGGCTAAAG CCTACAGTGT CCAGGGAGAC AAGTGGAGGG CCCTGGGCTA TGCAAAGGCC 
ATCAATGCTC TCAAGAGCTT CCACAAGCCT GTCAGCTCCT ACCAGGAGGC CTGTAGCATC 
CCGGGAATTG GCAAGCGGAT GGCGGAGAAG GTCATGGAGA TCCTGGAGAG TGGGCATCTG 
CGGAAGCTAG ACCACATCAG TGACAGTGTG CCTGTCTTGG AGCTCTTCTC CAACATCTGG 
GGAGCTGGGA CGAAGACTGC CCAGATGTGG TACCATCAGG GCTTCCGAAA CCTAGAAGAT 
CTCCAAAGCC TGGGCTCCCT GACCGCTCAG CAGGCCATTG GCTTGAAGCA CTACGATGAC 
TTCCTGGACC GCATGCCCAG GGAGGAGGCT GCGGAAATTG AGCAGACGGT CCGGATATCA 
GCCCAGGCCT TCAACCCTGG GCTGCTGTGT GTGGCTTGTG GCTCTTACCG ACGGGGGAAG 
ATGACGTGCG GGGATGTGGA TGTACTCATT ACTCACCCCG ATGGCCGATC CCACCGGGGC 
ATCTTCAGCT GCCTCCTTGA TAGCCTTCGG CAGCAAGGGT TCCTCACAGA TGACTTGGTG 
AGCCAGGAGG AGAACGGCCA ACAGCAGAAA TACCTGGGTG TGTGCCGGCT CCCAGGGCCC 
GGGAAGCGCC ACCGGCGACT GGACATCATC GTGGTACCCT ACTGTGAGTT TGCCTGTGCC 
CTGCTCTACT TCACCGGCTC TGCCCACTTC AACCGGTCCA TGAGAGCTCT GGCCAAGACC 
AAGGGCATGA GCCTGTCGGA GCATGCGCTC AGTGCAGCTG TAGTCCGGAA CAGCCAAGGT 
GTCAAGGTGG GGCCTGGACA AGTGCTGCCC ACCCCCACGG AGAAGGACGT CTTCAAGCAC 
TTAGGCCTGC CCTACCGGGA GCCAGCTGAA CGGGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Poll ( XM_011247301.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245603.1 Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant X1, mRNA.

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CloneID	OMu13441
Clone ID Related Accession (Same CDS sequence)	XM_011247301.2 , NM_001330506.1 , NM_020032.3 , NM_020032.2 , XM_006527232.1 , XM_011247301.1
Accession Version	XM_011247301.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA polymerase lambda isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247301.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTC AGGGCATCGT GAAGGCCTTT CCCAAGCGGA AGAAAAGTCA TGCAGATCTA 
TCTTCAAAAG CACTTGCAAA GATTCCCAAA AGGGAGGTGG GAGAAGCTAG AGGATGGCTG 
AGCTCCCTGA GAGCCCACAT TATGCCCGCT GGCATTGGAC GTGCCCGGGC TGAACTCTTT 
GAGAAGCAGA TTATCCACCA TGGCGGCCAG GTGTGCTCTG CCCAGGCCCC AGGAGTCACT 
CACATTGTGG TGGATGAAGA CATGGACTAT GAGCGGGCTC TCCGGCTCCT CAGGTTGCCC 
CAGCTACCCC CTGGTGCCCA GCTGGTGAAG TCCACCTGGC TGAGCTTGTG CCTGCAGGAG 
GGAAGGCTGA CAGACACGGA AGGATTCAGC CTTCCCATGC CCAAGAGGTC CTTGGATGAA 
CCACAGCCCA GCAAGTCAGG CCAAGATGCT TCTGCTCCTG GCACCCAGCG GGATTTACCC 
AGGACCACCC TTTCTCTTTC CCCTCCTCAC ACCAGAGCTG TATCTCCTCC CCCAACGGCA 
GAAAAGCCAT CAAGAACCCA AGCCCAGCTC AGCTCAGAGG ATGAAACCAG CGATGGAGAA 
GGGCCCCAGG TTAGCTCAGC AGATCTGCAA GCCTTGATCA CTGGGCACTA CCCCACTCCC 
CCTGAGGAAG ATGGTGGGCC TGACCCAGCA CCAGAAGCTC TGGATAAATG GGTCTGTGCA 
CAGCCCTCGA GCCAGAAGGC AACTAACTAC AATCTGCACA TCACAGAGAA GCTGGAAGTG 
CTGGCTAAAG CCTACAGTGT CCAGGGAGAC AAGTGGAGGG CCCTGGGCTA TGCAAAGGCC 
ATCAATGCTC TCAAGAGCTT CCACAAGCCT GTCAGCTCCT ACCAGGAGGC CTGTAGCATC 
CCGGGAATTG GCAAGCGGAT GGCGGAGAAG GTCATGGAGA TCCTGGAGAG TGGGCATCTG 
CGGAAGCTAG ACCACATCAG TGACAGTGTG CCTGTCTTGG AGCTCTTCTC CAACATCTGG 
GGAGCTGGGA CGAAGACTGC CCAGATGTGG TACCATCAGG GCTTCCGAAA CCTAGAAGAT 
CTCCAAAGCC TGGGCTCCCT GACCGCTCAG CAGGCCATTG GCTTGAAGCA CTACGATGAC 
TTCCTGGACC GCATGCCCAG GGAGGAGGCT GCGGAAATTG AGCAGACGGT CCGGATATCA 
GCCCAGGCCT TCAACCCTGG GCTGCTGTGT GTGGCTTGTG GCTCTTACCG ACGGGGGAAG 
ATGACGTGCG GGGATGTGGA TGTACTCATT ACTCACCCCG ATGGCCGATC CCACCGGGGC 
ATCTTCAGCT GCCTCCTTGA TAGCCTTCGG CAGCAAGGGT TCCTCACAGA TGACTTGGTG 
AGCCAGGAGG AGAACGGCCA ACAGCAGAAA TACCTGGGTG TGTGCCGGCT CCCAGGGCCC 
GGGAAGCGCC ACCGGCGACT GGACATCATC GTGGTACCCT ACTGTGAGTT TGCCTGTGCC 
CTGCTCTACT TCACCGGCTC TGCCCACTTC AACCGGTCCA TGAGAGCTCT GGCCAAGACC 
AAGGGCATGA GCCTGTCGGA GCATGCGCTC AGTGCAGCTG TAGTCCGGAA CAGCCAAGGT 
GTCAAGGTGG GGCCTGGACA AGTGCTGCCC ACCCCCACGG AGAAGGACGT CTTCAAGCAC 
TTAGGCCTGC CCTACCGGGA GCCAGCTGAA CGGGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245603.1
CDS	346..2067
Translation	MDPQGIVKAFPKRKKSHADLSSKALAKIPKREVGEARGWLSSLRAHIMPAGIGRARAELFEKQIIHHGGQVCSAQAPGVTHIVVDEDMDYERALRLLRLPQLPPGAQLVKSTWLSLCLQEGRLTDTEGFSLPMPKRSLDEPQPSKSGQDASAPGTQRDLPRTTLSLSPPHTRAVSPPPTAEKPSRTQAQLSSEDETSDGEGPQVSSADLQALITGHYPTPPEEDGGPDPAPEALDKWVCAQPSSQKATNYNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVSSYQEACSIPGIGKRMAEKVMEILESGHLRKLDHISDSVPVLELFSNIWGAGTKTAQMWYHQGFRNLEDLQSLGSLTAQQAIGLKHYDDFLDRMPREEAAEIEQTVRISAQAFNPGLLCVACGSYRRGKMTCGDVDVLITHPDGRSHRGIFSCLLDSLRQQGFLTDDLVSQEENGQQQKYLGVCRLPGPGKRHRRLDIIVVPYCEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSAAVVRNSQGVKVGPGQVLPTPTEKDVFKHLGLPYREPAERDW

Target ORF information:

RefSeq Version	XM_011247301.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247301.2

ORF Insert Sequence:

ATGGACCCTC AGGGCATCGT GAAGGCCTTT CCCAAGCGGA AGAAAAGTCA TGCAGATCTA 
TCTTCAAAAG CACTTGCAAA GATTCCCAAA AGGGAGGTGG GAGAAGCTAG AGGATGGCTG 
AGCTCCCTGA GAGCCCACAT TATGCCCGCT GGCATTGGAC GTGCCCGGGC TGAACTCTTT 
GAGAAGCAGA TTATCCACCA TGGCGGCCAG GTGTGCTCTG CCCAGGCCCC AGGAGTCACT 
CACATTGTGG TGGATGAAGA CATGGACTAT GAGCGGGCTC TCCGGCTCCT CAGGTTGCCC 
CAGCTACCCC CTGGTGCCCA GCTGGTGAAG TCCACCTGGC TGAGCTTGTG CCTGCAGGAG 
GGAAGGCTGA CAGACACGGA AGGATTCAGC CTTCCCATGC CCAAGAGGTC CTTGGATGAA 
CCACAGCCCA GCAAGTCAGG CCAAGATGCT TCTGCTCCTG GCACCCAGCG GGATTTACCC 
AGGACCACCC TTTCTCTTTC CCCTCCTCAC ACCAGAGCTG TATCTCCTCC CCCAACGGCA 
GAAAAGCCAT CAAGAACCCA AGCCCAGCTC AGCTCAGAGG ATGAAACCAG CGATGGAGAA 
GGGCCCCAGG TTAGCTCAGC AGATCTGCAA GCCTTGATCA CTGGGCACTA CCCCACTCCC 
CCTGAGGAAG ATGGTGGGCC TGACCCAGCA CCAGAAGCTC TGGATAAATG GGTCTGTGCA 
CAGCCCTCGA GCCAGAAGGC AACTAACTAC AATCTGCACA TCACAGAGAA GCTGGAAGTG 
CTGGCTAAAG CCTACAGTGT CCAGGGAGAC AAGTGGAGGG CCCTGGGCTA TGCAAAGGCC 
ATCAATGCTC TCAAGAGCTT CCACAAGCCT GTCAGCTCCT ACCAGGAGGC CTGTAGCATC 
CCGGGAATTG GCAAGCGGAT GGCGGAGAAG GTCATGGAGA TCCTGGAGAG TGGGCATCTG 
CGGAAGCTAG ACCACATCAG TGACAGTGTG CCTGTCTTGG AGCTCTTCTC CAACATCTGG 
GGAGCTGGGA CGAAGACTGC CCAGATGTGG TACCATCAGG GCTTCCGAAA CCTAGAAGAT 
CTCCAAAGCC TGGGCTCCCT GACCGCTCAG CAGGCCATTG GCTTGAAGCA CTACGATGAC 
TTCCTGGACC GCATGCCCAG GGAGGAGGCT GCGGAAATTG AGCAGACGGT CCGGATATCA 
GCCCAGGCCT TCAACCCTGG GCTGCTGTGT GTGGCTTGTG GCTCTTACCG ACGGGGGAAG 
ATGACGTGCG GGGATGTGGA TGTACTCATT ACTCACCCCG ATGGCCGATC CCACCGGGGC 
ATCTTCAGCT GCCTCCTTGA TAGCCTTCGG CAGCAAGGGT TCCTCACAGA TGACTTGGTG 
AGCCAGGAGG AGAACGGCCA ACAGCAGAAA TACCTGGGTG TGTGCCGGCT CCCAGGGCCC 
GGGAAGCGCC ACCGGCGACT GGACATCATC GTGGTACCCT ACTGTGAGTT TGCCTGTGCC 
CTGCTCTACT TCACCGGCTC TGCCCACTTC AACCGGTCCA TGAGAGCTCT GGCCAAGACC 
AAGGGCATGA GCCTGTCGGA GCATGCGCTC AGTGCAGCTG TAGTCCGGAA CAGCCAAGGT 
GTCAAGGTGG GGCCTGGACA AGTGCTGCCC ACCCCCACGG AGAAGGACGT CTTCAAGCAC 
TTAGGCCTGC CCTACCGGGA GCCAGCTGAA CGGGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Poll ( XM_006527232.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527295.1 Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu13441
Clone ID Related Accession (Same CDS sequence)	XM_011247301.2 , NM_001330506.1 , NM_020032.3 , NM_020032.2 , XM_006527232.1 , XM_011247301.1
Accession Version	XM_006527232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	DNA polymerase lambda isoform X1
Comment

ATGGACCCTC AGGGCATCGT GAAGGCCTTT CCCAAGCGGA AGAAAAGTCA TGCAGATCTA 
TCTTCAAAAG CACTTGCAAA GATTCCCAAA AGGGAGGTGG GAGAAGCTAG AGGATGGCTG 
AGCTCCCTGA GAGCCCACAT TATGCCCGCT GGCATTGGAC GTGCCCGGGC TGAACTCTTT 
GAGAAGCAGA TTATCCACCA TGGCGGCCAG GTGTGCTCTG CCCAGGCCCC AGGAGTCACT 
CACATTGTGG TGGATGAAGA CATGGACTAT GAGCGGGCTC TCCGGCTCCT CAGGTTGCCC 
CAGCTACCCC CTGGTGCCCA GCTGGTGAAG TCCACCTGGC TGAGCTTGTG CCTGCAGGAG 
GGAAGGCTGA CAGACACGGA AGGATTCAGC CTTCCCATGC CCAAGAGGTC CTTGGATGAA 
CCACAGCCCA GCAAGTCAGG CCAAGATGCT TCTGCTCCTG GCACCCAGCG GGATTTACCC 
AGGACCACCC TTTCTCTTTC CCCTCCTCAC ACCAGAGCTG TATCTCCTCC CCCAACGGCA 
GAAAAGCCAT CAAGAACCCA AGCCCAGCTC AGCTCAGAGG ATGAAACCAG CGATGGAGAA 
GGGCCCCAGG TTAGCTCAGC AGATCTGCAA GCCTTGATCA CTGGGCACTA CCCCACTCCC 
CCTGAGGAAG ATGGTGGGCC TGACCCAGCA CCAGAAGCTC TGGATAAATG GGTCTGTGCA 
CAGCCCTCGA GCCAGAAGGC AACTAACTAC AATCTGCACA TCACAGAGAA GCTGGAAGTG 
CTGGCTAAAG CCTACAGTGT CCAGGGAGAC AAGTGGAGGG CCCTGGGCTA TGCAAAGGCC 
ATCAATGCTC TCAAGAGCTT CCACAAGCCT GTCAGCTCCT ACCAGGAGGC CTGTAGCATC 
CCGGGAATTG GCAAGCGGAT GGCGGAGAAG GTCATGGAGA TCCTGGAGAG TGGGCATCTG 
CGGAAGCTAG ACCACATCAG TGACAGTGTG CCTGTCTTGG AGCTCTTCTC CAACATCTGG 
GGAGCTGGGA CGAAGACTGC CCAGATGTGG TACCATCAGG GCTTCCGAAA CCTAGAAGAT 
CTCCAAAGCC TGGGCTCCCT GACCGCTCAG CAGGCCATTG GCTTGAAGCA CTACGATGAC 
TTCCTGGACC GCATGCCCAG GGAGGAGGCT GCGGAAATTG AGCAGACGGT CCGGATATCA 
GCCCAGGCCT TCAACCCTGG GCTGCTGTGT GTGGCTTGTG GCTCTTACCG ACGGGGGAAG 
ATGACGTGCG GGGATGTGGA TGTACTCATT ACTCACCCCG ATGGCCGATC CCACCGGGGC 
ATCTTCAGCT GCCTCCTTGA TAGCCTTCGG CAGCAAGGGT TCCTCACAGA TGACTTGGTG 
AGCCAGGAGG AGAACGGCCA ACAGCAGAAA TACCTGGGTG TGTGCCGGCT CCCAGGGCCC 
GGGAAGCGCC ACCGGCGACT GGACATCATC GTGGTACCCT ACTGTGAGTT TGCCTGTGCC 
CTGCTCTACT TCACCGGCTC TGCCCACTTC AACCGGTCCA TGAGAGCTCT GGCCAAGACC 
AAGGGCATGA GCCTGTCGGA GCATGCGCTC AGTGCAGCTG TAGTCCGGAA CAGCCAAGGT 
GTCAAGGTGG GGCCTGGACA AGTGCTGCCC ACCCCCACGG AGAAGGACGT CTTCAAGCAC 
TTAGGCCTGC CCTACCGGGA GCCAGCTGAA CGGGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006527295.1

Translation

MDPQGIVKAFPKRKKSHADLSSKALAKIPKREVGEARGWLSSLRAHIMPAGIGRARAELFEKQIIHHGGQVCSAQAPGVTHIVVDEDMDYERALRLLRLPQLPPGAQLVKSTWLSLCLQEGRLTDTEGFSLPMPKRSLDEPQPSKSGQDASAPGTQRDLPRTTLSLSPPHTRAVSPPPTAEKPSRTQAQLSSEDETSDGEGPQVSSADLQALITGHYPTPPEEDGGPDPAPEALDKWVCAQPSSQKATNYNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVSSYQEACSIPGIGKRMAEKVMEILESGHLRKLDHISDSVPVLELFSNIWGAGTKTAQMWYHQGFRNLEDLQSLGSLTAQQAIGLKHYDDFLDRMPREEAAEIEQTVRISAQAFNPGLLCVACGSYRRGKMTCGDVDVLITHPDGRSHRGIFSCLLDSLRQQGFLTDDLVSQEENGQQQKYLGVCRLPGPGKRHRRLDIIVVPYCEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSAAVVRNSQGVKVGPGQVLPTPTEKDVFKHLGLPYREPAERDW

Target ORF information:

RefSeq Version	XM_006527232.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527232.1

ORF Insert Sequence:

ATGGACCCTC AGGGCATCGT GAAGGCCTTT CCCAAGCGGA AGAAAAGTCA TGCAGATCTA 
TCTTCAAAAG CACTTGCAAA GATTCCCAAA AGGGAGGTGG GAGAAGCTAG AGGATGGCTG 
AGCTCCCTGA GAGCCCACAT TATGCCCGCT GGCATTGGAC GTGCCCGGGC TGAACTCTTT 
GAGAAGCAGA TTATCCACCA TGGCGGCCAG GTGTGCTCTG CCCAGGCCCC AGGAGTCACT 
CACATTGTGG TGGATGAAGA CATGGACTAT GAGCGGGCTC TCCGGCTCCT CAGGTTGCCC 
CAGCTACCCC CTGGTGCCCA GCTGGTGAAG TCCACCTGGC TGAGCTTGTG CCTGCAGGAG 
GGAAGGCTGA CAGACACGGA AGGATTCAGC CTTCCCATGC CCAAGAGGTC CTTGGATGAA 
CCACAGCCCA GCAAGTCAGG CCAAGATGCT TCTGCTCCTG GCACCCAGCG GGATTTACCC 
AGGACCACCC TTTCTCTTTC CCCTCCTCAC ACCAGAGCTG TATCTCCTCC CCCAACGGCA 
GAAAAGCCAT CAAGAACCCA AGCCCAGCTC AGCTCAGAGG ATGAAACCAG CGATGGAGAA 
GGGCCCCAGG TTAGCTCAGC AGATCTGCAA GCCTTGATCA CTGGGCACTA CCCCACTCCC 
CCTGAGGAAG ATGGTGGGCC TGACCCAGCA CCAGAAGCTC TGGATAAATG GGTCTGTGCA 
CAGCCCTCGA GCCAGAAGGC AACTAACTAC AATCTGCACA TCACAGAGAA GCTGGAAGTG 
CTGGCTAAAG CCTACAGTGT CCAGGGAGAC AAGTGGAGGG CCCTGGGCTA TGCAAAGGCC 
ATCAATGCTC TCAAGAGCTT CCACAAGCCT GTCAGCTCCT ACCAGGAGGC CTGTAGCATC 
CCGGGAATTG GCAAGCGGAT GGCGGAGAAG GTCATGGAGA TCCTGGAGAG TGGGCATCTG 
CGGAAGCTAG ACCACATCAG TGACAGTGTG CCTGTCTTGG AGCTCTTCTC CAACATCTGG 
GGAGCTGGGA CGAAGACTGC CCAGATGTGG TACCATCAGG GCTTCCGAAA CCTAGAAGAT 
CTCCAAAGCC TGGGCTCCCT GACCGCTCAG CAGGCCATTG GCTTGAAGCA CTACGATGAC 
TTCCTGGACC GCATGCCCAG GGAGGAGGCT GCGGAAATTG AGCAGACGGT CCGGATATCA 
GCCCAGGCCT TCAACCCTGG GCTGCTGTGT GTGGCTTGTG GCTCTTACCG ACGGGGGAAG 
ATGACGTGCG GGGATGTGGA TGTACTCATT ACTCACCCCG ATGGCCGATC CCACCGGGGC 
ATCTTCAGCT GCCTCCTTGA TAGCCTTCGG CAGCAAGGGT TCCTCACAGA TGACTTGGTG 
AGCCAGGAGG AGAACGGCCA ACAGCAGAAA TACCTGGGTG TGTGCCGGCT CCCAGGGCCC 
GGGAAGCGCC ACCGGCGACT GGACATCATC GTGGTACCCT ACTGTGAGTT TGCCTGTGCC 
CTGCTCTACT TCACCGGCTC TGCCCACTTC AACCGGTCCA TGAGAGCTCT GGCCAAGACC 
AAGGGCATGA GCCTGTCGGA GCATGCGCTC AGTGCAGCTG TAGTCCGGAA CAGCCAAGGT 
GTCAAGGTGG GGCCTGGACA AGTGCTGCCC ACCCCCACGG AGAAGGACGT CTTCAAGCAC 
TTAGGCCTGC CCTACCGGGA GCCAGCTGAA CGGGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Poll ( XM_011247301.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245603.1 Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant X1, mRNA.

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CloneID	OMu13441
Clone ID Related Accession (Same CDS sequence)	XM_011247301.2 , NM_001330506.1 , NM_020032.3 , NM_020032.2 , XM_006527232.1 , XM_011247301.1
Accession Version	XM_011247301.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	DNA polymerase lambda isoform X1
Comment

ATGGACCCTC AGGGCATCGT GAAGGCCTTT CCCAAGCGGA AGAAAAGTCA TGCAGATCTA 
TCTTCAAAAG CACTTGCAAA GATTCCCAAA AGGGAGGTGG GAGAAGCTAG AGGATGGCTG 
AGCTCCCTGA GAGCCCACAT TATGCCCGCT GGCATTGGAC GTGCCCGGGC TGAACTCTTT 
GAGAAGCAGA TTATCCACCA TGGCGGCCAG GTGTGCTCTG CCCAGGCCCC AGGAGTCACT 
CACATTGTGG TGGATGAAGA CATGGACTAT GAGCGGGCTC TCCGGCTCCT CAGGTTGCCC 
CAGCTACCCC CTGGTGCCCA GCTGGTGAAG TCCACCTGGC TGAGCTTGTG CCTGCAGGAG 
GGAAGGCTGA CAGACACGGA AGGATTCAGC CTTCCCATGC CCAAGAGGTC CTTGGATGAA 
CCACAGCCCA GCAAGTCAGG CCAAGATGCT TCTGCTCCTG GCACCCAGCG GGATTTACCC 
AGGACCACCC TTTCTCTTTC CCCTCCTCAC ACCAGAGCTG TATCTCCTCC CCCAACGGCA 
GAAAAGCCAT CAAGAACCCA AGCCCAGCTC AGCTCAGAGG ATGAAACCAG CGATGGAGAA 
GGGCCCCAGG TTAGCTCAGC AGATCTGCAA GCCTTGATCA CTGGGCACTA CCCCACTCCC 
CCTGAGGAAG ATGGTGGGCC TGACCCAGCA CCAGAAGCTC TGGATAAATG GGTCTGTGCA 
CAGCCCTCGA GCCAGAAGGC AACTAACTAC AATCTGCACA TCACAGAGAA GCTGGAAGTG 
CTGGCTAAAG CCTACAGTGT CCAGGGAGAC AAGTGGAGGG CCCTGGGCTA TGCAAAGGCC 
ATCAATGCTC TCAAGAGCTT CCACAAGCCT GTCAGCTCCT ACCAGGAGGC CTGTAGCATC 
CCGGGAATTG GCAAGCGGAT GGCGGAGAAG GTCATGGAGA TCCTGGAGAG TGGGCATCTG 
CGGAAGCTAG ACCACATCAG TGACAGTGTG CCTGTCTTGG AGCTCTTCTC CAACATCTGG 
GGAGCTGGGA CGAAGACTGC CCAGATGTGG TACCATCAGG GCTTCCGAAA CCTAGAAGAT 
CTCCAAAGCC TGGGCTCCCT GACCGCTCAG CAGGCCATTG GCTTGAAGCA CTACGATGAC 
TTCCTGGACC GCATGCCCAG GGAGGAGGCT GCGGAAATTG AGCAGACGGT CCGGATATCA 
GCCCAGGCCT TCAACCCTGG GCTGCTGTGT GTGGCTTGTG GCTCTTACCG ACGGGGGAAG 
ATGACGTGCG GGGATGTGGA TGTACTCATT ACTCACCCCG ATGGCCGATC CCACCGGGGC 
ATCTTCAGCT GCCTCCTTGA TAGCCTTCGG CAGCAAGGGT TCCTCACAGA TGACTTGGTG 
AGCCAGGAGG AGAACGGCCA ACAGCAGAAA TACCTGGGTG TGTGCCGGCT CCCAGGGCCC 
GGGAAGCGCC ACCGGCGACT GGACATCATC GTGGTACCCT ACTGTGAGTT TGCCTGTGCC 
CTGCTCTACT TCACCGGCTC TGCCCACTTC AACCGGTCCA TGAGAGCTCT GGCCAAGACC 
AAGGGCATGA GCCTGTCGGA GCATGCGCTC AGTGCAGCTG TAGTCCGGAA CAGCCAAGGT 
GTCAAGGTGG GGCCTGGACA AGTGCTGCCC ACCCCCACGG AGAAGGACGT CTTCAAGCAC 
TTAGGCCTGC CCTACCGGGA GCCAGCTGAA CGGGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011245603.1

Translation

MDPQGIVKAFPKRKKSHADLSSKALAKIPKREVGEARGWLSSLRAHIMPAGIGRARAELFEKQIIHHGGQVCSAQAPGVTHIVVDEDMDYERALRLLRLPQLPPGAQLVKSTWLSLCLQEGRLTDTEGFSLPMPKRSLDEPQPSKSGQDASAPGTQRDLPRTTLSLSPPHTRAVSPPPTAEKPSRTQAQLSSEDETSDGEGPQVSSADLQALITGHYPTPPEEDGGPDPAPEALDKWVCAQPSSQKATNYNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVSSYQEACSIPGIGKRMAEKVMEILESGHLRKLDHISDSVPVLELFSNIWGAGTKTAQMWYHQGFRNLEDLQSLGSLTAQQAIGLKHYDDFLDRMPREEAAEIEQTVRISAQAFNPGLLCVACGSYRRGKMTCGDVDVLITHPDGRSHRGIFSCLLDSLRQQGFLTDDLVSQEENGQQQKYLGVCRLPGPGKRHRRLDIIVVPYCEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSAAVVRNSQGVKVGPGQVLPTPTEKDVFKHLGLPYREPAERDW

Target ORF information:

RefSeq Version	XM_011247301.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus polymerase (DNA directed), lambda (Poll), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247301.1

ORF Insert Sequence:

ATGGACCCTC AGGGCATCGT GAAGGCCTTT CCCAAGCGGA AGAAAAGTCA TGCAGATCTA 
TCTTCAAAAG CACTTGCAAA GATTCCCAAA AGGGAGGTGG GAGAAGCTAG AGGATGGCTG 
AGCTCCCTGA GAGCCCACAT TATGCCCGCT GGCATTGGAC GTGCCCGGGC TGAACTCTTT 
GAGAAGCAGA TTATCCACCA TGGCGGCCAG GTGTGCTCTG CCCAGGCCCC AGGAGTCACT 
CACATTGTGG TGGATGAAGA CATGGACTAT GAGCGGGCTC TCCGGCTCCT CAGGTTGCCC 
CAGCTACCCC CTGGTGCCCA GCTGGTGAAG TCCACCTGGC TGAGCTTGTG CCTGCAGGAG 
GGAAGGCTGA CAGACACGGA AGGATTCAGC CTTCCCATGC CCAAGAGGTC CTTGGATGAA 
CCACAGCCCA GCAAGTCAGG CCAAGATGCT TCTGCTCCTG GCACCCAGCG GGATTTACCC 
AGGACCACCC TTTCTCTTTC CCCTCCTCAC ACCAGAGCTG TATCTCCTCC CCCAACGGCA 
GAAAAGCCAT CAAGAACCCA AGCCCAGCTC AGCTCAGAGG ATGAAACCAG CGATGGAGAA 
GGGCCCCAGG TTAGCTCAGC AGATCTGCAA GCCTTGATCA CTGGGCACTA CCCCACTCCC 
CCTGAGGAAG ATGGTGGGCC TGACCCAGCA CCAGAAGCTC TGGATAAATG GGTCTGTGCA 
CAGCCCTCGA GCCAGAAGGC AACTAACTAC AATCTGCACA TCACAGAGAA GCTGGAAGTG 
CTGGCTAAAG CCTACAGTGT CCAGGGAGAC AAGTGGAGGG CCCTGGGCTA TGCAAAGGCC 
ATCAATGCTC TCAAGAGCTT CCACAAGCCT GTCAGCTCCT ACCAGGAGGC CTGTAGCATC 
CCGGGAATTG GCAAGCGGAT GGCGGAGAAG GTCATGGAGA TCCTGGAGAG TGGGCATCTG 
CGGAAGCTAG ACCACATCAG TGACAGTGTG CCTGTCTTGG AGCTCTTCTC CAACATCTGG 
GGAGCTGGGA CGAAGACTGC CCAGATGTGG TACCATCAGG GCTTCCGAAA CCTAGAAGAT 
CTCCAAAGCC TGGGCTCCCT GACCGCTCAG CAGGCCATTG GCTTGAAGCA CTACGATGAC 
TTCCTGGACC GCATGCCCAG GGAGGAGGCT GCGGAAATTG AGCAGACGGT CCGGATATCA 
GCCCAGGCCT TCAACCCTGG GCTGCTGTGT GTGGCTTGTG GCTCTTACCG ACGGGGGAAG 
ATGACGTGCG GGGATGTGGA TGTACTCATT ACTCACCCCG ATGGCCGATC CCACCGGGGC 
ATCTTCAGCT GCCTCCTTGA TAGCCTTCGG CAGCAAGGGT TCCTCACAGA TGACTTGGTG 
AGCCAGGAGG AGAACGGCCA ACAGCAGAAA TACCTGGGTG TGTGCCGGCT CCCAGGGCCC 
GGGAAGCGCC ACCGGCGACT GGACATCATC GTGGTACCCT ACTGTGAGTT TGCCTGTGCC 
CTGCTCTACT TCACCGGCTC TGCCCACTTC AACCGGTCCA TGAGAGCTCT GGCCAAGACC 
AAGGGCATGA GCCTGTCGGA GCATGCGCTC AGTGCAGCTG TAGTCCGGAA CAGCCAAGGT 
GTCAAGGTGG GGCCTGGACA AGTGCTGCCC ACCCCCACGG AGAAGGACGT CTTCAAGCAC 
TTAGGCCTGC CCTACCGGGA GCCAGCTGAA CGGGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increased neuronal death and disturbed axonal growth in the Pol?-deficient mouse embryonic retina.
Scientific reports625928(2016 May)
Baleriola J,?lvarez-Lindo N,de la Villa P,Bernad A,Blanco L,Su?rez T,de la Rosa EJ

Essential role for polymerase specialization in cellular nonhomologous end joining.
Proceedings of the National Academy of Sciences of the United States of America112(33)E4537-45(2015 Aug)
Pryor JM,Waters CA,Aza A,Asagoshi K,Strom C,Mieczkowski PA,Blanco L,Ramsden DA

DNA polymerases ? and ? and their roles in cell.
DNA repair29112-26(2015 May)
Belousova EA,Lavrik OI

Structure-function studies of DNA polymerase ?.
Biochemistry53(17)2781-92(2014 May)
Bebenek K,Pedersen LC,Kunkel TA

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Poll cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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