Cilk1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cilk1
Entrez Gene ID	56542
Full Name	intestinal cell kinase
Synonyms	2210420N10Rik,AI848300,Mrk
General protein information	Preferred Names intestinal cell kinase Names serine/threonine-protein kinase ICK MAK-related kinase mICK
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 E1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006511305.3	XP_006511368.1	serine/threonine-protein kinase ICK isoform X2
NM_019987.2	NP_064371.2	serine/threonine-protein kinase ICK
XM_030244465.1	XP_030100325.1	serine/threonine-protein kinase ICK isoform X2
XM_006511304.2	XP_006511367.1	serine/threonine-protein kinase ICK isoform X1
XM_030244466.1	XP_030100326.1	serine/threonine-protein kinase ICK isoform X2
NM_001163780.1	NP_001157252.1	serine/threonine-protein kinase ICK

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ICK	NP_001092357.1
Xenopus tropicalis (tropical clawed frog)	ick	XP_002934912.2
Mus musculus (house mouse)	Ick	NP_064371.2
Gallus gallus (chicken)	ICK	XP_004940534.1
Homo sapiens (human)	ICK	NP_055735.1
Rattus norvegicus (Norway rat)	Ick	NP_620241.1
	LOC711865	XP_002808434.1
Pan troglodytes (chimpanzee)	ICK	XP_001154965.2
Canis lupus familiaris (dog)	ICK	XP_005627533.1

Gene Ontology
Bibliography

Related articles in PubMed

A Murine Model for Human ECO Syndrome Reveals a Critical Role of Intestinal Cell Kinase in Skeletal Development.
Ding M, Jin L, Xie L, Park SH, Tong Y, Wu D, Chhabra AB, Fu Z, Li X
Calcified tissue international(2017 Nov)

An essential role of intestinal cell kinase in lung development is linked to the perinatal lethality of human ECO syndrome.
Tong Y, Park SH, Wu D, Xu W, Guillot SJ, Jin L, Li X, Wang Y, Lin CS, Fu Z
FEBS letters591(9)1247-1257(2017 05)

Ick Ciliary Kinase Is Essential for Planar Cell Polarity Formation in Inner Ear Hair Cells and Hearing Function.
Okamoto S, Chaya T, Omori Y, Kuwahara R, Kubo S, Sakaguchi H, Furukawa T
The Journal of neuroscience : the official journal of the Society for Neuroscience37(8)2073-2085(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate an essential role for intestinal cell kinase (ICK) in lung development and advances the mechanistic understanding of endocrine-cerebro-osteodysplasia (ECO) syndrome.
Title: An essential role of intestinal cell kinase in lung development is linked to the perinatal lethality of human ECO syndrome.

The results of this study found that loss of Ick leads to abnormal localization of the IFT component in kinocilia, PCP defects in HCs, and hearing dysfunction.
Title: Ick Ciliary Kinase Is Essential for Planar Cell Polarity Formation in Inner Ear Hair Cells and Hearing Function.

Data indicate that ciliary defects caused by a lack of functional intestinal cell kinase (ICK) leads to abnormal Sonic hedgehog (Shh) signaling, resulting in congenital disorders such as endocrine-cerebro-osteodysplasia (ECO) syndrome .
Title: Intestinal cell kinase, a protein associated with endocrine-cerebro-osteodysplasia syndrome, is a key regulator of cilia length and Hedgehog signaling.

ICK is a Kif3a kinase and essential for proper ciliogenesis in development by regulating ciliary transport at the tip of cilia.
Title: ICK is essential for cell type-specific ciliogenesis and the regulation of ciliary transport.

findings suggest that increased ICK expression/activity in response to protein deprivation likely provides a novel protective mechanism to limit apoptosis and support compensatory mucosal growth under nutritional stress.
Title: Intestinal cell kinase is a novel participant in intestinal cell signaling responses to protein malnutrition.

The following Cilk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cilk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09435	NM_001163780.1 Latest version!	Mus musculus ciliogenesis associated kinase 1 (Cilk1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu09435	NM_019987.2 Latest version!	Mus musculus ciliogenesis associated kinase 1 (Cilk1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu09435	XM_006511304.2 Latest version!	Mus musculus intestinal cell kinase (Ick), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu42887	XM_006511305.3 Latest version!	Mus musculus intestinal cell kinase (Ick), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu42887	XM_030244466.1 Latest version!	Mus musculus intestinal cell kinase (Ick), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu42887	XM_030244465.1 Latest version!	Mus musculus intestinal cell kinase (Ick), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cilk1 ( NM_019987.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064371.2 Mus musculus ciliogenesis associated kinase 1 (Cilk1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu09435
Clone ID Related Accession (Same CDS sequence)	NM_019987.2 , XM_006511304.2 , NM_001163780.1
Accession Version	NM_019987.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase ICK
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC159313.3, AK087484.1, AK173068.1 and BM935223.1. On Aug 6, 2009 this sequence version replaced NM_019987.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.131334.1, SRR1660815.88993.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATAGAT ACACAACGAT CAAGCAGCTT GGGGATGGGA CCTACGGCTC TGTCCTGCTG 
GGGAGAAGCA TTGAGTCTGG AGAACTGATT GCCATTAAAA AAATGAAAAG AAAGTTTTAC 
TCCTGGGAAG AATGCATGAA CCTTCGGGAG GTTAAGTCTT TAAAGAAGCT TAATCATGCC 
AATATCGTAA AGTTAAAAGA GGTTATCAGG GAAAACGACC ATCTTTACTT TATCTTCGAG 
TACATGAAGG AAAACCTTTA CCAGCTCATT AAAGAAAGAA ATAAGTTGTT TCCAGAGTCT 
GCTATAAGGA ATATAATGTA CCAGATATTG CAAGGACTGG CATTCATTCA CAAACACGGT 
TTCTTCCACC GGGACTTAAA ACCCGAGAAC CTCCTCTGCA TGGGACCAGA ACTTGTGAAA 
ATCGCCGACT TTGGATTGGC TCGAGAAATC CGATCAAGAC CTCCGTACAC AGACTATGTG 
TCTACTAGAT GGTACAGGGC TCCAGAGGTA CTGCTGAGAT CCACCAACTA CAGCTCCCCC 
ATTGACATCT GGGCGGTAGG CTGCATCATG GCGGAGGTGT ACACGCTCCG GCCCCTTTTC 
CCTGGGGCCA GTGAAATTGA CACAATTTTC AAAATTTGCC AAGTGTTGGG AACACCAAAG 
AAGACTGACT GGCCTGAAGG CTACCAGCTG TCAAGTGCTA TGAATTTCCT CTGGCCCCAG 
TGCATACCCA ATAACTTAAA GACTCTAATC CCAAATGCTA GCAGCGAAGC CATTCAGCTC 
CTGAGAGACT TGCTTCAGTG GGATCCCAAG AAACGACCAA CCGCTAGTCA GGCTCTTCGA 
TATCCTTACT TCCAGATCGG ACACCCACTG GGCATCATCA GCAAGGACTC AGGAAAACCA 
CAGAGAGAGG TCCAGGACAA GACAGGCCCT CCTCCTTACA TCAAGCCAGC CCCTCCTGCC 
CAGGCCCCGG CCAAGGCATA TACGCTGATT TCTTCACGAC CAAGCCAAGC CAGTCAGCCC 
CCTCAGCATT CTGTGCACCC CTACAAAGGC GATGTCTCTA GGACTGAGCA GCTGAGCCAT 
GTTCAGGAGG GCAAGCCAAG CCCTCCATTC TTCCCATCCC TCCACAATAA GAATCTTCAG 
CCAAAAATCC TAGCCAGCCT GGAACAGAAA AATGGTGAAA TCAAGCCAAA GAGCAGGAGA 
AGATGGGGTC TCATCTCCAG GTCGACAAAG GGTTCTGACG ACTGGGCCGA CTTGGATGAC 
TTAGACTTCA GTCCCTCTCT CACCAGGATA GATGTAAAAA ACAAGAAGAG ACAGAGTGAT 
GACACCCTCT GCAGGTTTGA AAGTGTTCTG GACCTGAAGC CATCTGAGTC AGTGGGTACA 
GGAACCACTG TCTCCACGCA GGCTTCCTCG CAGAGGAGAG ACACGCCGAC CCTGCAGTCC 
TCAGCAAAGC AGCACTACTT GAAGCACTCT CGCTACTTGC CTGGAATAAA TATAAGAAAT 
GGCGTGCTCC CAAATCCAGG CAAGGACTTT CTTCCATCAA ATTCATGGTC CAGTTCCGGC 
TTGTCTGGAA AATCTTCAGG AACAGTATCA GTAGTGAGCA AAATAACTTC AGTTGGCTCA 
GGCTCTGCGA GCTCTAGTGG ACTGACTGGA AGCTACATTC CTTCCTTTCT GAAAAAGGAA 
ATCGGTTCTG TCATGCAGAG GGTTCAGCTG GCGCCCCTCG CAGCCCCTCC CCCCGGCTAC 
TCTTCCCTCA AGGCCGTGAG ACCTCACCCG GGGAGGCCTT TCTTTCACAC CCAGCCCAGG 
AGCACGCCCG GCTTGATCCC CCGACCTCCA GCTGCTCAGC CTGTGCACGG CAGGATAGAC 
TGGTCTTCCA AGTACCCATC CCGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064371.2
CDS	395..2284
Translation	MNRYTTIKQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEECMNLREVKSLKKLNHANIVKLKEVIRENDHLYFIFEYMKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSRPPYTDYVSTRWYRAPEVLLRSTNYSSPIDIWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKTDWPEGYQLSSAMNFLWPQCIPNNLKTLIPNASSEAIQLLRDLLQWDPKKRPTASQALRYPYFQIGHPLGIISKDSGKPQREVQDKTGPPPYIKPAPPAQAPAKAYTLISSRPSQASQPPQHSVHPYKGDVSRTEQLSHVQEGKPSPPFFPSLHNKNLQPKILASLEQKNGEIKPKSRRRWGLISRSTKGSDDWADLDDLDFSPSLTRIDVKNKKRQSDDTLCRFESVLDLKPSESVGTGTTVSTQASSQRRDTPTLQSSAKQHYLKHSRYLPGINIRNGVLPNPGKDFLPSNSWSSSGLSGKSSGTVSVVSKITSVGSGSASSSGLTGSYIPSFLKKEIGSVMQRVQLAPLAAPPPGYSSLKAVRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRIDWSSKYPSRR

Target ORF information:

RefSeq Version	NM_019987.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ciliogenesis associated kinase 1 (Cilk1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019987.2

ORF Insert Sequence:

ATGAATAGAT ACACAACGAT CAAGCAGCTT GGGGATGGGA CCTACGGCTC TGTCCTGCTG 
GGGAGAAGCA TTGAGTCTGG AGAACTGATT GCCATTAAAA AAATGAAAAG AAAGTTTTAC 
TCCTGGGAAG AATGCATGAA CCTTCGGGAG GTTAAGTCTT TAAAGAAGCT TAATCATGCC 
AATATCGTAA AGTTAAAAGA GGTTATCAGG GAAAACGACC ATCTTTACTT TATCTTCGAG 
TACATGAAGG AAAACCTTTA CCAGCTCATT AAAGAAAGAA ATAAGTTGTT TCCAGAGTCT 
GCTATAAGGA ATATAATGTA CCAGATATTG CAAGGACTGG CATTCATTCA CAAACACGGT 
TTCTTCCACC GGGACTTAAA ACCCGAGAAC CTCCTCTGCA TGGGACCAGA ACTTGTGAAA 
ATCGCCGACT TTGGATTGGC TCGAGAAATC CGATCAAGAC CTCCGTACAC AGACTATGTG 
TCTACTAGAT GGTACAGGGC TCCAGAGGTA CTGCTGAGAT CCACCAACTA CAGCTCCCCC 
ATTGACATCT GGGCGGTAGG CTGCATCATG GCGGAGGTGT ACACGCTCCG GCCCCTTTTC 
CCTGGGGCCA GTGAAATTGA CACAATTTTC AAAATTTGCC AAGTGTTGGG AACACCAAAG 
AAGACTGACT GGCCTGAAGG CTACCAGCTG TCAAGTGCTA TGAATTTCCT CTGGCCCCAG 
TGCATACCCA ATAACTTAAA GACTCTAATC CCAAATGCTA GCAGCGAAGC CATTCAGCTC 
CTGAGAGACT TGCTTCAGTG GGATCCCAAG AAACGACCAA CCGCTAGTCA GGCTCTTCGA 
TATCCTTACT TCCAGATCGG ACACCCACTG GGCATCATCA GCAAGGACTC AGGAAAACCA 
CAGAGAGAGG TCCAGGACAA GACAGGCCCT CCTCCTTACA TCAAGCCAGC CCCTCCTGCC 
CAGGCCCCGG CCAAGGCATA TACGCTGATT TCTTCACGAC CAAGCCAAGC CAGTCAGCCC 
CCTCAGCATT CTGTGCACCC CTACAAAGGC GATGTCTCTA GGACTGAGCA GCTGAGCCAT 
GTTCAGGAGG GCAAGCCAAG CCCTCCATTC TTCCCATCCC TCCACAATAA GAATCTTCAG 
CCAAAAATCC TAGCCAGCCT GGAACAGAAA AATGGTGAAA TCAAGCCAAA GAGCAGGAGA 
AGATGGGGTC TCATCTCCAG GTCGACAAAG GGTTCTGACG ACTGGGCCGA CTTGGATGAC 
TTAGACTTCA GTCCCTCTCT CACCAGGATA GATGTAAAAA ACAAGAAGAG ACAGAGTGAT 
GACACCCTCT GCAGGTTTGA AAGTGTTCTG GACCTGAAGC CATCTGAGTC AGTGGGTACA 
GGAACCACTG TCTCCACGCA GGCTTCCTCG CAGAGGAGAG ACACGCCGAC CCTGCAGTCC 
TCAGCAAAGC AGCACTACTT GAAGCACTCT CGCTACTTGC CTGGAATAAA TATAAGAAAT 
GGCGTGCTCC CAAATCCAGG CAAGGACTTT CTTCCATCAA ATTCATGGTC CAGTTCCGGC 
TTGTCTGGAA AATCTTCAGG AACAGTATCA GTAGTGAGCA AAATAACTTC AGTTGGCTCA 
GGCTCTGCGA GCTCTAGTGG ACTGACTGGA AGCTACATTC CTTCCTTTCT GAAAAAGGAA 
ATCGGTTCTG TCATGCAGAG GGTTCAGCTG GCGCCCCTCG CAGCCCCTCC CCCCGGCTAC 
TCTTCCCTCA AGGCCGTGAG ACCTCACCCG GGGAGGCCTT TCTTTCACAC CCAGCCCAGG 
AGCACGCCCG GCTTGATCCC CCGACCTCCA GCTGCTCAGC CTGTGCACGG CAGGATAGAC 
TGGTCTTCCA AGTACCCATC CCGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cilk1 ( NM_001163780.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157252.1 Mus musculus ciliogenesis associated kinase 1 (Cilk1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09435
Clone ID Related Accession (Same CDS sequence)	NM_019987.2 , XM_006511304.2 , NM_001163780.1
Accession Version	NM_001163780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase ICK
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC159313.3. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK154185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATAGAT ACACAACGAT CAAGCAGCTT GGGGATGGGA CCTACGGCTC TGTCCTGCTG 
GGGAGAAGCA TTGAGTCTGG AGAACTGATT GCCATTAAAA AAATGAAAAG AAAGTTTTAC 
TCCTGGGAAG AATGCATGAA CCTTCGGGAG GTTAAGTCTT TAAAGAAGCT TAATCATGCC 
AATATCGTAA AGTTAAAAGA GGTTATCAGG GAAAACGACC ATCTTTACTT TATCTTCGAG 
TACATGAAGG AAAACCTTTA CCAGCTCATT AAAGAAAGAA ATAAGTTGTT TCCAGAGTCT 
GCTATAAGGA ATATAATGTA CCAGATATTG CAAGGACTGG CATTCATTCA CAAACACGGT 
TTCTTCCACC GGGACTTAAA ACCCGAGAAC CTCCTCTGCA TGGGACCAGA ACTTGTGAAA 
ATCGCCGACT TTGGATTGGC TCGAGAAATC CGATCAAGAC CTCCGTACAC AGACTATGTG 
TCTACTAGAT GGTACAGGGC TCCAGAGGTA CTGCTGAGAT CCACCAACTA CAGCTCCCCC 
ATTGACATCT GGGCGGTAGG CTGCATCATG GCGGAGGTGT ACACGCTCCG GCCCCTTTTC 
CCTGGGGCCA GTGAAATTGA CACAATTTTC AAAATTTGCC AAGTGTTGGG AACACCAAAG 
AAGACTGACT GGCCTGAAGG CTACCAGCTG TCAAGTGCTA TGAATTTCCT CTGGCCCCAG 
TGCATACCCA ATAACTTAAA GACTCTAATC CCAAATGCTA GCAGCGAAGC CATTCAGCTC 
CTGAGAGACT TGCTTCAGTG GGATCCCAAG AAACGACCAA CCGCTAGTCA GGCTCTTCGA 
TATCCTTACT TCCAGATCGG ACACCCACTG GGCATCATCA GCAAGGACTC AGGAAAACCA 
CAGAGAGAGG TCCAGGACAA GACAGGCCCT CCTCCTTACA TCAAGCCAGC CCCTCCTGCC 
CAGGCCCCGG CCAAGGCATA TACGCTGATT TCTTCACGAC CAAGCCAAGC CAGTCAGCCC 
CCTCAGCATT CTGTGCACCC CTACAAAGGC GATGTCTCTA GGACTGAGCA GCTGAGCCAT 
GTTCAGGAGG GCAAGCCAAG CCCTCCATTC TTCCCATCCC TCCACAATAA GAATCTTCAG 
CCAAAAATCC TAGCCAGCCT GGAACAGAAA AATGGTGAAA TCAAGCCAAA GAGCAGGAGA 
AGATGGGGTC TCATCTCCAG GTCGACAAAG GGTTCTGACG ACTGGGCCGA CTTGGATGAC 
TTAGACTTCA GTCCCTCTCT CACCAGGATA GATGTAAAAA ACAAGAAGAG ACAGAGTGAT 
GACACCCTCT GCAGGTTTGA AAGTGTTCTG GACCTGAAGC CATCTGAGTC AGTGGGTACA 
GGAACCACTG TCTCCACGCA GGCTTCCTCG CAGAGGAGAG ACACGCCGAC CCTGCAGTCC 
TCAGCAAAGC AGCACTACTT GAAGCACTCT CGCTACTTGC CTGGAATAAA TATAAGAAAT 
GGCGTGCTCC CAAATCCAGG CAAGGACTTT CTTCCATCAA ATTCATGGTC CAGTTCCGGC 
TTGTCTGGAA AATCTTCAGG AACAGTATCA GTAGTGAGCA AAATAACTTC AGTTGGCTCA 
GGCTCTGCGA GCTCTAGTGG ACTGACTGGA AGCTACATTC CTTCCTTTCT GAAAAAGGAA 
ATCGGTTCTG TCATGCAGAG GGTTCAGCTG GCGCCCCTCG CAGCCCCTCC CCCCGGCTAC 
TCTTCCCTCA AGGCCGTGAG ACCTCACCCG GGGAGGCCTT TCTTTCACAC CCAGCCCAGG 
AGCACGCCCG GCTTGATCCC CCGACCTCCA GCTGCTCAGC CTGTGCACGG CAGGATAGAC 
TGGTCTTCCA AGTACCCATC CCGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157252.1
CDS	467..2356
Translation	MNRYTTIKQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEECMNLREVKSLKKLNHANIVKLKEVIRENDHLYFIFEYMKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSRPPYTDYVSTRWYRAPEVLLRSTNYSSPIDIWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKTDWPEGYQLSSAMNFLWPQCIPNNLKTLIPNASSEAIQLLRDLLQWDPKKRPTASQALRYPYFQIGHPLGIISKDSGKPQREVQDKTGPPPYIKPAPPAQAPAKAYTLISSRPSQASQPPQHSVHPYKGDVSRTEQLSHVQEGKPSPPFFPSLHNKNLQPKILASLEQKNGEIKPKSRRRWGLISRSTKGSDDWADLDDLDFSPSLTRIDVKNKKRQSDDTLCRFESVLDLKPSESVGTGTTVSTQASSQRRDTPTLQSSAKQHYLKHSRYLPGINIRNGVLPNPGKDFLPSNSWSSSGLSGKSSGTVSVVSKITSVGSGSASSSGLTGSYIPSFLKKEIGSVMQRVQLAPLAAPPPGYSSLKAVRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRIDWSSKYPSRR

Target ORF information:

RefSeq Version	NM_001163780.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ciliogenesis associated kinase 1 (Cilk1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163780.1

ORF Insert Sequence:

ATGAATAGAT ACACAACGAT CAAGCAGCTT GGGGATGGGA CCTACGGCTC TGTCCTGCTG 
GGGAGAAGCA TTGAGTCTGG AGAACTGATT GCCATTAAAA AAATGAAAAG AAAGTTTTAC 
TCCTGGGAAG AATGCATGAA CCTTCGGGAG GTTAAGTCTT TAAAGAAGCT TAATCATGCC 
AATATCGTAA AGTTAAAAGA GGTTATCAGG GAAAACGACC ATCTTTACTT TATCTTCGAG 
TACATGAAGG AAAACCTTTA CCAGCTCATT AAAGAAAGAA ATAAGTTGTT TCCAGAGTCT 
GCTATAAGGA ATATAATGTA CCAGATATTG CAAGGACTGG CATTCATTCA CAAACACGGT 
TTCTTCCACC GGGACTTAAA ACCCGAGAAC CTCCTCTGCA TGGGACCAGA ACTTGTGAAA 
ATCGCCGACT TTGGATTGGC TCGAGAAATC CGATCAAGAC CTCCGTACAC AGACTATGTG 
TCTACTAGAT GGTACAGGGC TCCAGAGGTA CTGCTGAGAT CCACCAACTA CAGCTCCCCC 
ATTGACATCT GGGCGGTAGG CTGCATCATG GCGGAGGTGT ACACGCTCCG GCCCCTTTTC 
CCTGGGGCCA GTGAAATTGA CACAATTTTC AAAATTTGCC AAGTGTTGGG AACACCAAAG 
AAGACTGACT GGCCTGAAGG CTACCAGCTG TCAAGTGCTA TGAATTTCCT CTGGCCCCAG 
TGCATACCCA ATAACTTAAA GACTCTAATC CCAAATGCTA GCAGCGAAGC CATTCAGCTC 
CTGAGAGACT TGCTTCAGTG GGATCCCAAG AAACGACCAA CCGCTAGTCA GGCTCTTCGA 
TATCCTTACT TCCAGATCGG ACACCCACTG GGCATCATCA GCAAGGACTC AGGAAAACCA 
CAGAGAGAGG TCCAGGACAA GACAGGCCCT CCTCCTTACA TCAAGCCAGC CCCTCCTGCC 
CAGGCCCCGG CCAAGGCATA TACGCTGATT TCTTCACGAC CAAGCCAAGC CAGTCAGCCC 
CCTCAGCATT CTGTGCACCC CTACAAAGGC GATGTCTCTA GGACTGAGCA GCTGAGCCAT 
GTTCAGGAGG GCAAGCCAAG CCCTCCATTC TTCCCATCCC TCCACAATAA GAATCTTCAG 
CCAAAAATCC TAGCCAGCCT GGAACAGAAA AATGGTGAAA TCAAGCCAAA GAGCAGGAGA 
AGATGGGGTC TCATCTCCAG GTCGACAAAG GGTTCTGACG ACTGGGCCGA CTTGGATGAC 
TTAGACTTCA GTCCCTCTCT CACCAGGATA GATGTAAAAA ACAAGAAGAG ACAGAGTGAT 
GACACCCTCT GCAGGTTTGA AAGTGTTCTG GACCTGAAGC CATCTGAGTC AGTGGGTACA 
GGAACCACTG TCTCCACGCA GGCTTCCTCG CAGAGGAGAG ACACGCCGAC CCTGCAGTCC 
TCAGCAAAGC AGCACTACTT GAAGCACTCT CGCTACTTGC CTGGAATAAA TATAAGAAAT 
GGCGTGCTCC CAAATCCAGG CAAGGACTTT CTTCCATCAA ATTCATGGTC CAGTTCCGGC 
TTGTCTGGAA AATCTTCAGG AACAGTATCA GTAGTGAGCA AAATAACTTC AGTTGGCTCA 
GGCTCTGCGA GCTCTAGTGG ACTGACTGGA AGCTACATTC CTTCCTTTCT GAAAAAGGAA 
ATCGGTTCTG TCATGCAGAG GGTTCAGCTG GCGCCCCTCG CAGCCCCTCC CCCCGGCTAC 
TCTTCCCTCA AGGCCGTGAG ACCTCACCCG GGGAGGCCTT TCTTTCACAC CCAGCCCAGG 
AGCACGCCCG GCTTGATCCC CCGACCTCCA GCTGCTCAGC CTGTGCACGG CAGGATAGAC 
TGGTCTTCCA AGTACCCATC CCGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cilk1 ( XM_006511304.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511367.1 Mus musculus intestinal cell kinase (Ick), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu09435
Clone ID Related Accession (Same CDS sequence)	NM_019987.2 , XM_006511304.2 , NM_001163780.1
Accession Version	XM_006511304.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase ICK isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006511304.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAGAT ACACAACGAT CAAGCAGCTT GGGGATGGGA CCTACGGCTC TGTCCTGCTG 
GGGAGAAGCA TTGAGTCTGG AGAACTGATT GCCATTAAAA AAATGAAAAG AAAGTTTTAC 
TCCTGGGAAG AATGCATGAA CCTTCGGGAG GTTAAGTCTT TAAAGAAGCT TAATCATGCC 
AATATCGTAA AGTTAAAAGA GGTTATCAGG GAAAACGACC ATCTTTACTT TATCTTCGAG 
TACATGAAGG AAAACCTTTA CCAGCTCATT AAAGAAAGAA ATAAGTTGTT TCCAGAGTCT 
GCTATAAGGA ATATAATGTA CCAGATATTG CAAGGACTGG CATTCATTCA CAAACACGGT 
TTCTTCCACC GGGACTTAAA ACCCGAGAAC CTCCTCTGCA TGGGACCAGA ACTTGTGAAA 
ATCGCCGACT TTGGATTGGC TCGAGAAATC CGATCAAGAC CTCCGTACAC AGACTATGTG 
TCTACTAGAT GGTACAGGGC TCCAGAGGTA CTGCTGAGAT CCACCAACTA CAGCTCCCCC 
ATTGACATCT GGGCGGTAGG CTGCATCATG GCGGAGGTGT ACACGCTCCG GCCCCTTTTC 
CCTGGGGCCA GTGAAATTGA CACAATTTTC AAAATTTGCC AAGTGTTGGG AACACCAAAG 
AAGACTGACT GGCCTGAAGG CTACCAGCTG TCAAGTGCTA TGAATTTCCT CTGGCCCCAG 
TGCATACCCA ATAACTTAAA GACTCTAATC CCAAATGCTA GCAGCGAAGC CATTCAGCTC 
CTGAGAGACT TGCTTCAGTG GGATCCCAAG AAACGACCAA CCGCTAGTCA GGCTCTTCGA 
TATCCTTACT TCCAGATCGG ACACCCACTG GGCATCATCA GCAAGGACTC AGGAAAACCA 
CAGAGAGAGG TCCAGGACAA GACAGGCCCT CCTCCTTACA TCAAGCCAGC CCCTCCTGCC 
CAGGCCCCGG CCAAGGCATA TACGCTGATT TCTTCACGAC CAAGCCAAGC CAGTCAGCCC 
CCTCAGCATT CTGTGCACCC CTACAAAGGC GATGTCTCTA GGACTGAGCA GCTGAGCCAT 
GTTCAGGAGG GCAAGCCAAG CCCTCCATTC TTCCCATCCC TCCACAATAA GAATCTTCAG 
CCAAAAATCC TAGCCAGCCT GGAACAGAAA AATGGTGAAA TCAAGCCAAA GAGCAGGAGA 
AGATGGGGTC TCATCTCCAG GTCGACAAAG GGTTCTGACG ACTGGGCCGA CTTGGATGAC 
TTAGACTTCA GTCCCTCTCT CACCAGGATA GATGTAAAAA ACAAGAAGAG ACAGAGTGAT 
GACACCCTCT GCAGGTTTGA AAGTGTTCTG GACCTGAAGC CATCTGAGTC AGTGGGTACA 
GGAACCACTG TCTCCACGCA GGCTTCCTCG CAGAGGAGAG ACACGCCGAC CCTGCAGTCC 
TCAGCAAAGC AGCACTACTT GAAGCACTCT CGCTACTTGC CTGGAATAAA TATAAGAAAT 
GGCGTGCTCC CAAATCCAGG CAAGGACTTT CTTCCATCAA ATTCATGGTC CAGTTCCGGC 
TTGTCTGGAA AATCTTCAGG AACAGTATCA GTAGTGAGCA AAATAACTTC AGTTGGCTCA 
GGCTCTGCGA GCTCTAGTGG ACTGACTGGA AGCTACATTC CTTCCTTTCT GAAAAAGGAA 
ATCGGTTCTG TCATGCAGAG GGTTCAGCTG GCGCCCCTCG CAGCCCCTCC CCCCGGCTAC 
TCTTCCCTCA AGGCCGTGAG ACCTCACCCG GGGAGGCCTT TCTTTCACAC CCAGCCCAGG 
AGCACGCCCG GCTTGATCCC CCGACCTCCA GCTGCTCAGC CTGTGCACGG CAGGATAGAC 
TGGTCTTCCA AGTACCCATC CCGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511367.1
CDS	300..2189
Translation	MNRYTTIKQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEECMNLREVKSLKKLNHANIVKLKEVIRENDHLYFIFEYMKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSRPPYTDYVSTRWYRAPEVLLRSTNYSSPIDIWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKTDWPEGYQLSSAMNFLWPQCIPNNLKTLIPNASSEAIQLLRDLLQWDPKKRPTASQALRYPYFQIGHPLGIISKDSGKPQREVQDKTGPPPYIKPAPPAQAPAKAYTLISSRPSQASQPPQHSVHPYKGDVSRTEQLSHVQEGKPSPPFFPSLHNKNLQPKILASLEQKNGEIKPKSRRRWGLISRSTKGSDDWADLDDLDFSPSLTRIDVKNKKRQSDDTLCRFESVLDLKPSESVGTGTTVSTQASSQRRDTPTLQSSAKQHYLKHSRYLPGINIRNGVLPNPGKDFLPSNSWSSSGLSGKSSGTVSVVSKITSVGSGSASSSGLTGSYIPSFLKKEIGSVMQRVQLAPLAAPPPGYSSLKAVRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRIDWSSKYPSRR

Target ORF information:

RefSeq Version	XM_006511304.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus intestinal cell kinase (Ick), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511304.2

ORF Insert Sequence:

ATGAATAGAT ACACAACGAT CAAGCAGCTT GGGGATGGGA CCTACGGCTC TGTCCTGCTG 
GGGAGAAGCA TTGAGTCTGG AGAACTGATT GCCATTAAAA AAATGAAAAG AAAGTTTTAC 
TCCTGGGAAG AATGCATGAA CCTTCGGGAG GTTAAGTCTT TAAAGAAGCT TAATCATGCC 
AATATCGTAA AGTTAAAAGA GGTTATCAGG GAAAACGACC ATCTTTACTT TATCTTCGAG 
TACATGAAGG AAAACCTTTA CCAGCTCATT AAAGAAAGAA ATAAGTTGTT TCCAGAGTCT 
GCTATAAGGA ATATAATGTA CCAGATATTG CAAGGACTGG CATTCATTCA CAAACACGGT 
TTCTTCCACC GGGACTTAAA ACCCGAGAAC CTCCTCTGCA TGGGACCAGA ACTTGTGAAA 
ATCGCCGACT TTGGATTGGC TCGAGAAATC CGATCAAGAC CTCCGTACAC AGACTATGTG 
TCTACTAGAT GGTACAGGGC TCCAGAGGTA CTGCTGAGAT CCACCAACTA CAGCTCCCCC 
ATTGACATCT GGGCGGTAGG CTGCATCATG GCGGAGGTGT ACACGCTCCG GCCCCTTTTC 
CCTGGGGCCA GTGAAATTGA CACAATTTTC AAAATTTGCC AAGTGTTGGG AACACCAAAG 
AAGACTGACT GGCCTGAAGG CTACCAGCTG TCAAGTGCTA TGAATTTCCT CTGGCCCCAG 
TGCATACCCA ATAACTTAAA GACTCTAATC CCAAATGCTA GCAGCGAAGC CATTCAGCTC 
CTGAGAGACT TGCTTCAGTG GGATCCCAAG AAACGACCAA CCGCTAGTCA GGCTCTTCGA 
TATCCTTACT TCCAGATCGG ACACCCACTG GGCATCATCA GCAAGGACTC AGGAAAACCA 
CAGAGAGAGG TCCAGGACAA GACAGGCCCT CCTCCTTACA TCAAGCCAGC CCCTCCTGCC 
CAGGCCCCGG CCAAGGCATA TACGCTGATT TCTTCACGAC CAAGCCAAGC CAGTCAGCCC 
CCTCAGCATT CTGTGCACCC CTACAAAGGC GATGTCTCTA GGACTGAGCA GCTGAGCCAT 
GTTCAGGAGG GCAAGCCAAG CCCTCCATTC TTCCCATCCC TCCACAATAA GAATCTTCAG 
CCAAAAATCC TAGCCAGCCT GGAACAGAAA AATGGTGAAA TCAAGCCAAA GAGCAGGAGA 
AGATGGGGTC TCATCTCCAG GTCGACAAAG GGTTCTGACG ACTGGGCCGA CTTGGATGAC 
TTAGACTTCA GTCCCTCTCT CACCAGGATA GATGTAAAAA ACAAGAAGAG ACAGAGTGAT 
GACACCCTCT GCAGGTTTGA AAGTGTTCTG GACCTGAAGC CATCTGAGTC AGTGGGTACA 
GGAACCACTG TCTCCACGCA GGCTTCCTCG CAGAGGAGAG ACACGCCGAC CCTGCAGTCC 
TCAGCAAAGC AGCACTACTT GAAGCACTCT CGCTACTTGC CTGGAATAAA TATAAGAAAT 
GGCGTGCTCC CAAATCCAGG CAAGGACTTT CTTCCATCAA ATTCATGGTC CAGTTCCGGC 
TTGTCTGGAA AATCTTCAGG AACAGTATCA GTAGTGAGCA AAATAACTTC AGTTGGCTCA 
GGCTCTGCGA GCTCTAGTGG ACTGACTGGA AGCTACATTC CTTCCTTTCT GAAAAAGGAA 
ATCGGTTCTG TCATGCAGAG GGTTCAGCTG GCGCCCCTCG CAGCCCCTCC CCCCGGCTAC 
TCTTCCCTCA AGGCCGTGAG ACCTCACCCG GGGAGGCCTT TCTTTCACAC CCAGCCCAGG 
AGCACGCCCG GCTTGATCCC CCGACCTCCA GCTGCTCAGC CTGTGCACGG CAGGATAGAC 
TGGTCTTCCA AGTACCCATC CCGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cilk1 ( XM_006511305.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511368.1 Mus musculus intestinal cell kinase (Ick), transcript variant X2, mRNA.

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CloneID	OMu42887
Clone ID Related Accession (Same CDS sequence)	XM_006511305.3 , XM_030244465.1 , XM_030244466.1
Accession Version	XM_006511305.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase ICK isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511305.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAA ACCTTTACCA GCTCATTAAA GAAAGAAATA AGTTGTTTCC AGAGTCTGCT 
ATAAGGAATA TAATGTACCA GATATTGCAA GGACTGGCAT TCATTCACAA ACACGGTTTC 
TTCCACCGGG ACTTAAAACC CGAGAACCTC CTCTGCATGG GACCAGAACT TGTGAAAATC 
GCCGACTTTG GATTGGCTCG AGAAATCCGA TCAAGACCTC CGTACACAGA CTATGTGTCT 
ACTAGATGGT ACAGGGCTCC AGAGGTACTG CTGAGATCCA CCAACTACAG CTCCCCCATT 
GACATCTGGG CGGTAGGCTG CATCATGGCG GAGGTGTACA CGCTCCGGCC CCTTTTCCCT 
GGGGCCAGTG AAATTGACAC AATTTTCAAA ATTTGCCAAG TGTTGGGAAC ACCAAAGAAG 
ACTGACTGGC CTGAAGGCTA CCAGCTGTCA AGTGCTATGA ATTTCCTCTG GCCCCAGTGC 
ATACCCAATA ACTTAAAGAC TCTAATCCCA AATGCTAGCA GCGAAGCCAT TCAGCTCCTG 
AGAGACTTGC TTCAGTGGGA TCCCAAGAAA CGACCAACCG CTAGTCAGGC TCTTCGATAT 
CCTTACTTCC AGATCGGACA CCCACTGGGC ATCATCAGCA AGGACTCAGG AAAACCACAG 
AGAGAGGTCC AGGACAAGAC AGGCCCTCCT CCTTACATCA AGCCAGCCCC TCCTGCCCAG 
GCCCCGGCCA AGGCATATAC GCTGATTTCT TCACGACCAA GCCAAGCCAG TCAGCCCCCT 
CAGCATTCTG TGCACCCCTA CAAAGGCGAT GTCTCTAGGA CTGAGCAGCT GAGCCATGTT 
CAGGAGGGCA AGCCAAGCCC TCCATTCTTC CCATCCCTCC ACAATAAGAA TCTTCAGCCA 
AAAATCCTAG CCAGCCTGGA ACAGAAAAAT GGTGAAATCA AGCCAAAGAG CAGGAGAAGA 
TGGGGTCTCA TCTCCAGGTC GACAAAGGGT TCTGACGACT GGGCCGACTT GGATGACTTA 
GACTTCAGTC CCTCTCTCAC CAGGATAGAT GTAAAAAACA AGAAGAGACA GAGTGATGAC 
ACCCTCTGCA GGTTTGAAAG TGTTCTGGAC CTGAAGCCAT CTGAGTCAGT GGGTACAGGA 
ACCACTGTCT CCACGCAGGC TTCCTCGCAG AGGAGAGACA CGCCGACCCT GCAGTCCTCA 
GCAAAGCAGC ACTACTTGAA GCACTCTCGC TACTTGCCTG GAATAAATAT AAGAAATGGC 
GTGCTCCCAA ATCCAGGCAA GGACTTTCTT CCATCAAATT CATGGTCCAG TTCCGGCTTG 
TCTGGAAAAT CTTCAGGAAC AGTATCAGTA GTGAGCAAAA TAACTTCAGT TGGCTCAGGC 
TCTGCGAGCT CTAGTGGACT GACTGGAAGC TACATTCCTT CCTTTCTGAA AAAGGAAATC 
GGTTCTGTCA TGCAGAGGGT TCAGCTGGCG CCCCTCGCAG CCCCTCCCCC CGGCTACTCT 
TCCCTCAAGG CCGTGAGACC TCACCCGGGG AGGCCTTTCT TTCACACCCA GCCCAGGAGC 
ACGCCCGGCT TGATCCCCCG ACCTCCAGCT GCTCAGCCTG TGCACGGCAG GATAGACTGG 
TCTTCCAAGT ACCCATCCCG GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511368.1
CDS	153..1799
Translation	MKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSRPPYTDYVSTRWYRAPEVLLRSTNYSSPIDIWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKTDWPEGYQLSSAMNFLWPQCIPNNLKTLIPNASSEAIQLLRDLLQWDPKKRPTASQALRYPYFQIGHPLGIISKDSGKPQREVQDKTGPPPYIKPAPPAQAPAKAYTLISSRPSQASQPPQHSVHPYKGDVSRTEQLSHVQEGKPSPPFFPSLHNKNLQPKILASLEQKNGEIKPKSRRRWGLISRSTKGSDDWADLDDLDFSPSLTRIDVKNKKRQSDDTLCRFESVLDLKPSESVGTGTTVSTQASSQRRDTPTLQSSAKQHYLKHSRYLPGINIRNGVLPNPGKDFLPSNSWSSSGLSGKSSGTVSVVSKITSVGSGSASSSGLTGSYIPSFLKKEIGSVMQRVQLAPLAAPPPGYSSLKAVRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRIDWSSKYPSRR

Target ORF information:

RefSeq Version	XM_006511305.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus intestinal cell kinase (Ick), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511305.3

ORF Insert Sequence:

ATGAAGGAAA ACCTTTACCA GCTCATTAAA GAAAGAAATA AGTTGTTTCC AGAGTCTGCT 
ATAAGGAATA TAATGTACCA GATATTGCAA GGACTGGCAT TCATTCACAA ACACGGTTTC 
TTCCACCGGG ACTTAAAACC CGAGAACCTC CTCTGCATGG GACCAGAACT TGTGAAAATC 
GCCGACTTTG GATTGGCTCG AGAAATCCGA TCAAGACCTC CGTACACAGA CTATGTGTCT 
ACTAGATGGT ACAGGGCTCC AGAGGTACTG CTGAGATCCA CCAACTACAG CTCCCCCATT 
GACATCTGGG CGGTAGGCTG CATCATGGCG GAGGTGTACA CGCTCCGGCC CCTTTTCCCT 
GGGGCCAGTG AAATTGACAC AATTTTCAAA ATTTGCCAAG TGTTGGGAAC ACCAAAGAAG 
ACTGACTGGC CTGAAGGCTA CCAGCTGTCA AGTGCTATGA ATTTCCTCTG GCCCCAGTGC 
ATACCCAATA ACTTAAAGAC TCTAATCCCA AATGCTAGCA GCGAAGCCAT TCAGCTCCTG 
AGAGACTTGC TTCAGTGGGA TCCCAAGAAA CGACCAACCG CTAGTCAGGC TCTTCGATAT 
CCTTACTTCC AGATCGGACA CCCACTGGGC ATCATCAGCA AGGACTCAGG AAAACCACAG 
AGAGAGGTCC AGGACAAGAC AGGCCCTCCT CCTTACATCA AGCCAGCCCC TCCTGCCCAG 
GCCCCGGCCA AGGCATATAC GCTGATTTCT TCACGACCAA GCCAAGCCAG TCAGCCCCCT 
CAGCATTCTG TGCACCCCTA CAAAGGCGAT GTCTCTAGGA CTGAGCAGCT GAGCCATGTT 
CAGGAGGGCA AGCCAAGCCC TCCATTCTTC CCATCCCTCC ACAATAAGAA TCTTCAGCCA 
AAAATCCTAG CCAGCCTGGA ACAGAAAAAT GGTGAAATCA AGCCAAAGAG CAGGAGAAGA 
TGGGGTCTCA TCTCCAGGTC GACAAAGGGT TCTGACGACT GGGCCGACTT GGATGACTTA 
GACTTCAGTC CCTCTCTCAC CAGGATAGAT GTAAAAAACA AGAAGAGACA GAGTGATGAC 
ACCCTCTGCA GGTTTGAAAG TGTTCTGGAC CTGAAGCCAT CTGAGTCAGT GGGTACAGGA 
ACCACTGTCT CCACGCAGGC TTCCTCGCAG AGGAGAGACA CGCCGACCCT GCAGTCCTCA 
GCAAAGCAGC ACTACTTGAA GCACTCTCGC TACTTGCCTG GAATAAATAT AAGAAATGGC 
GTGCTCCCAA ATCCAGGCAA GGACTTTCTT CCATCAAATT CATGGTCCAG TTCCGGCTTG 
TCTGGAAAAT CTTCAGGAAC AGTATCAGTA GTGAGCAAAA TAACTTCAGT TGGCTCAGGC 
TCTGCGAGCT CTAGTGGACT GACTGGAAGC TACATTCCTT CCTTTCTGAA AAAGGAAATC 
GGTTCTGTCA TGCAGAGGGT TCAGCTGGCG CCCCTCGCAG CCCCTCCCCC CGGCTACTCT 
TCCCTCAAGG CCGTGAGACC TCACCCGGGG AGGCCTTTCT TTCACACCCA GCCCAGGAGC 
ACGCCCGGCT TGATCCCCCG ACCTCCAGCT GCTCAGCCTG TGCACGGCAG GATAGACTGG 
TCTTCCAAGT ACCCATCCCG GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cilk1 ( XM_030244465.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100325.1 Mus musculus intestinal cell kinase (Ick), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu42887
Clone ID Related Accession (Same CDS sequence)	XM_006511305.3 , XM_030244465.1 , XM_030244466.1
Accession Version	XM_030244465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase ICK isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAA ACCTTTACCA GCTCATTAAA GAAAGAAATA AGTTGTTTCC AGAGTCTGCT 
ATAAGGAATA TAATGTACCA GATATTGCAA GGACTGGCAT TCATTCACAA ACACGGTTTC 
TTCCACCGGG ACTTAAAACC CGAGAACCTC CTCTGCATGG GACCAGAACT TGTGAAAATC 
GCCGACTTTG GATTGGCTCG AGAAATCCGA TCAAGACCTC CGTACACAGA CTATGTGTCT 
ACTAGATGGT ACAGGGCTCC AGAGGTACTG CTGAGATCCA CCAACTACAG CTCCCCCATT 
GACATCTGGG CGGTAGGCTG CATCATGGCG GAGGTGTACA CGCTCCGGCC CCTTTTCCCT 
GGGGCCAGTG AAATTGACAC AATTTTCAAA ATTTGCCAAG TGTTGGGAAC ACCAAAGAAG 
ACTGACTGGC CTGAAGGCTA CCAGCTGTCA AGTGCTATGA ATTTCCTCTG GCCCCAGTGC 
ATACCCAATA ACTTAAAGAC TCTAATCCCA AATGCTAGCA GCGAAGCCAT TCAGCTCCTG 
AGAGACTTGC TTCAGTGGGA TCCCAAGAAA CGACCAACCG CTAGTCAGGC TCTTCGATAT 
CCTTACTTCC AGATCGGACA CCCACTGGGC ATCATCAGCA AGGACTCAGG AAAACCACAG 
AGAGAGGTCC AGGACAAGAC AGGCCCTCCT CCTTACATCA AGCCAGCCCC TCCTGCCCAG 
GCCCCGGCCA AGGCATATAC GCTGATTTCT TCACGACCAA GCCAAGCCAG TCAGCCCCCT 
CAGCATTCTG TGCACCCCTA CAAAGGCGAT GTCTCTAGGA CTGAGCAGCT GAGCCATGTT 
CAGGAGGGCA AGCCAAGCCC TCCATTCTTC CCATCCCTCC ACAATAAGAA TCTTCAGCCA 
AAAATCCTAG CCAGCCTGGA ACAGAAAAAT GGTGAAATCA AGCCAAAGAG CAGGAGAAGA 
TGGGGTCTCA TCTCCAGGTC GACAAAGGGT TCTGACGACT GGGCCGACTT GGATGACTTA 
GACTTCAGTC CCTCTCTCAC CAGGATAGAT GTAAAAAACA AGAAGAGACA GAGTGATGAC 
ACCCTCTGCA GGTTTGAAAG TGTTCTGGAC CTGAAGCCAT CTGAGTCAGT GGGTACAGGA 
ACCACTGTCT CCACGCAGGC TTCCTCGCAG AGGAGAGACA CGCCGACCCT GCAGTCCTCA 
GCAAAGCAGC ACTACTTGAA GCACTCTCGC TACTTGCCTG GAATAAATAT AAGAAATGGC 
GTGCTCCCAA ATCCAGGCAA GGACTTTCTT CCATCAAATT CATGGTCCAG TTCCGGCTTG 
TCTGGAAAAT CTTCAGGAAC AGTATCAGTA GTGAGCAAAA TAACTTCAGT TGGCTCAGGC 
TCTGCGAGCT CTAGTGGACT GACTGGAAGC TACATTCCTT CCTTTCTGAA AAAGGAAATC 
GGTTCTGTCA TGCAGAGGGT TCAGCTGGCG CCCCTCGCAG CCCCTCCCCC CGGCTACTCT 
TCCCTCAAGG CCGTGAGACC TCACCCGGGG AGGCCTTTCT TTCACACCCA GCCCAGGAGC 
ACGCCCGGCT TGATCCCCCG ACCTCCAGCT GCTCAGCCTG TGCACGGCAG GATAGACTGG 
TCTTCCAAGT ACCCATCCCG GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100325.1
CDS	603..2249
Translation	MKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSRPPYTDYVSTRWYRAPEVLLRSTNYSSPIDIWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKTDWPEGYQLSSAMNFLWPQCIPNNLKTLIPNASSEAIQLLRDLLQWDPKKRPTASQALRYPYFQIGHPLGIISKDSGKPQREVQDKTGPPPYIKPAPPAQAPAKAYTLISSRPSQASQPPQHSVHPYKGDVSRTEQLSHVQEGKPSPPFFPSLHNKNLQPKILASLEQKNGEIKPKSRRRWGLISRSTKGSDDWADLDDLDFSPSLTRIDVKNKKRQSDDTLCRFESVLDLKPSESVGTGTTVSTQASSQRRDTPTLQSSAKQHYLKHSRYLPGINIRNGVLPNPGKDFLPSNSWSSSGLSGKSSGTVSVVSKITSVGSGSASSSGLTGSYIPSFLKKEIGSVMQRVQLAPLAAPPPGYSSLKAVRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRIDWSSKYPSRR

Target ORF information:

RefSeq Version	XM_030244465.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus intestinal cell kinase (Ick), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244465.1

ORF Insert Sequence:

ATGAAGGAAA ACCTTTACCA GCTCATTAAA GAAAGAAATA AGTTGTTTCC AGAGTCTGCT 
ATAAGGAATA TAATGTACCA GATATTGCAA GGACTGGCAT TCATTCACAA ACACGGTTTC 
TTCCACCGGG ACTTAAAACC CGAGAACCTC CTCTGCATGG GACCAGAACT TGTGAAAATC 
GCCGACTTTG GATTGGCTCG AGAAATCCGA TCAAGACCTC CGTACACAGA CTATGTGTCT 
ACTAGATGGT ACAGGGCTCC AGAGGTACTG CTGAGATCCA CCAACTACAG CTCCCCCATT 
GACATCTGGG CGGTAGGCTG CATCATGGCG GAGGTGTACA CGCTCCGGCC CCTTTTCCCT 
GGGGCCAGTG AAATTGACAC AATTTTCAAA ATTTGCCAAG TGTTGGGAAC ACCAAAGAAG 
ACTGACTGGC CTGAAGGCTA CCAGCTGTCA AGTGCTATGA ATTTCCTCTG GCCCCAGTGC 
ATACCCAATA ACTTAAAGAC TCTAATCCCA AATGCTAGCA GCGAAGCCAT TCAGCTCCTG 
AGAGACTTGC TTCAGTGGGA TCCCAAGAAA CGACCAACCG CTAGTCAGGC TCTTCGATAT 
CCTTACTTCC AGATCGGACA CCCACTGGGC ATCATCAGCA AGGACTCAGG AAAACCACAG 
AGAGAGGTCC AGGACAAGAC AGGCCCTCCT CCTTACATCA AGCCAGCCCC TCCTGCCCAG 
GCCCCGGCCA AGGCATATAC GCTGATTTCT TCACGACCAA GCCAAGCCAG TCAGCCCCCT 
CAGCATTCTG TGCACCCCTA CAAAGGCGAT GTCTCTAGGA CTGAGCAGCT GAGCCATGTT 
CAGGAGGGCA AGCCAAGCCC TCCATTCTTC CCATCCCTCC ACAATAAGAA TCTTCAGCCA 
AAAATCCTAG CCAGCCTGGA ACAGAAAAAT GGTGAAATCA AGCCAAAGAG CAGGAGAAGA 
TGGGGTCTCA TCTCCAGGTC GACAAAGGGT TCTGACGACT GGGCCGACTT GGATGACTTA 
GACTTCAGTC CCTCTCTCAC CAGGATAGAT GTAAAAAACA AGAAGAGACA GAGTGATGAC 
ACCCTCTGCA GGTTTGAAAG TGTTCTGGAC CTGAAGCCAT CTGAGTCAGT GGGTACAGGA 
ACCACTGTCT CCACGCAGGC TTCCTCGCAG AGGAGAGACA CGCCGACCCT GCAGTCCTCA 
GCAAAGCAGC ACTACTTGAA GCACTCTCGC TACTTGCCTG GAATAAATAT AAGAAATGGC 
GTGCTCCCAA ATCCAGGCAA GGACTTTCTT CCATCAAATT CATGGTCCAG TTCCGGCTTG 
TCTGGAAAAT CTTCAGGAAC AGTATCAGTA GTGAGCAAAA TAACTTCAGT TGGCTCAGGC 
TCTGCGAGCT CTAGTGGACT GACTGGAAGC TACATTCCTT CCTTTCTGAA AAAGGAAATC 
GGTTCTGTCA TGCAGAGGGT TCAGCTGGCG CCCCTCGCAG CCCCTCCCCC CGGCTACTCT 
TCCCTCAAGG CCGTGAGACC TCACCCGGGG AGGCCTTTCT TTCACACCCA GCCCAGGAGC 
ACGCCCGGCT TGATCCCCCG ACCTCCAGCT GCTCAGCCTG TGCACGGCAG GATAGACTGG 
TCTTCCAAGT ACCCATCCCG GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cilk1 ( XM_030244466.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100326.1 Mus musculus intestinal cell kinase (Ick), transcript variant X3, mRNA.

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CloneID	OMu42887
Clone ID Related Accession (Same CDS sequence)	XM_006511305.3 , XM_030244465.1 , XM_030244466.1
Accession Version	XM_030244466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase ICK isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAA ACCTTTACCA GCTCATTAAA GAAAGAAATA AGTTGTTTCC AGAGTCTGCT 
ATAAGGAATA TAATGTACCA GATATTGCAA GGACTGGCAT TCATTCACAA ACACGGTTTC 
TTCCACCGGG ACTTAAAACC CGAGAACCTC CTCTGCATGG GACCAGAACT TGTGAAAATC 
GCCGACTTTG GATTGGCTCG AGAAATCCGA TCAAGACCTC CGTACACAGA CTATGTGTCT 
ACTAGATGGT ACAGGGCTCC AGAGGTACTG CTGAGATCCA CCAACTACAG CTCCCCCATT 
GACATCTGGG CGGTAGGCTG CATCATGGCG GAGGTGTACA CGCTCCGGCC CCTTTTCCCT 
GGGGCCAGTG AAATTGACAC AATTTTCAAA ATTTGCCAAG TGTTGGGAAC ACCAAAGAAG 
ACTGACTGGC CTGAAGGCTA CCAGCTGTCA AGTGCTATGA ATTTCCTCTG GCCCCAGTGC 
ATACCCAATA ACTTAAAGAC TCTAATCCCA AATGCTAGCA GCGAAGCCAT TCAGCTCCTG 
AGAGACTTGC TTCAGTGGGA TCCCAAGAAA CGACCAACCG CTAGTCAGGC TCTTCGATAT 
CCTTACTTCC AGATCGGACA CCCACTGGGC ATCATCAGCA AGGACTCAGG AAAACCACAG 
AGAGAGGTCC AGGACAAGAC AGGCCCTCCT CCTTACATCA AGCCAGCCCC TCCTGCCCAG 
GCCCCGGCCA AGGCATATAC GCTGATTTCT TCACGACCAA GCCAAGCCAG TCAGCCCCCT 
CAGCATTCTG TGCACCCCTA CAAAGGCGAT GTCTCTAGGA CTGAGCAGCT GAGCCATGTT 
CAGGAGGGCA AGCCAAGCCC TCCATTCTTC CCATCCCTCC ACAATAAGAA TCTTCAGCCA 
AAAATCCTAG CCAGCCTGGA ACAGAAAAAT GGTGAAATCA AGCCAAAGAG CAGGAGAAGA 
TGGGGTCTCA TCTCCAGGTC GACAAAGGGT TCTGACGACT GGGCCGACTT GGATGACTTA 
GACTTCAGTC CCTCTCTCAC CAGGATAGAT GTAAAAAACA AGAAGAGACA GAGTGATGAC 
ACCCTCTGCA GGTTTGAAAG TGTTCTGGAC CTGAAGCCAT CTGAGTCAGT GGGTACAGGA 
ACCACTGTCT CCACGCAGGC TTCCTCGCAG AGGAGAGACA CGCCGACCCT GCAGTCCTCA 
GCAAAGCAGC ACTACTTGAA GCACTCTCGC TACTTGCCTG GAATAAATAT AAGAAATGGC 
GTGCTCCCAA ATCCAGGCAA GGACTTTCTT CCATCAAATT CATGGTCCAG TTCCGGCTTG 
TCTGGAAAAT CTTCAGGAAC AGTATCAGTA GTGAGCAAAA TAACTTCAGT TGGCTCAGGC 
TCTGCGAGCT CTAGTGGACT GACTGGAAGC TACATTCCTT CCTTTCTGAA AAAGGAAATC 
GGTTCTGTCA TGCAGAGGGT TCAGCTGGCG CCCCTCGCAG CCCCTCCCCC CGGCTACTCT 
TCCCTCAAGG CCGTGAGACC TCACCCGGGG AGGCCTTTCT TTCACACCCA GCCCAGGAGC 
ACGCCCGGCT TGATCCCCCG ACCTCCAGCT GCTCAGCCTG TGCACGGCAG GATAGACTGG 
TCTTCCAAGT ACCCATCCCG GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100326.1
CDS	767..2413
Translation	MKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSRPPYTDYVSTRWYRAPEVLLRSTNYSSPIDIWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKTDWPEGYQLSSAMNFLWPQCIPNNLKTLIPNASSEAIQLLRDLLQWDPKKRPTASQALRYPYFQIGHPLGIISKDSGKPQREVQDKTGPPPYIKPAPPAQAPAKAYTLISSRPSQASQPPQHSVHPYKGDVSRTEQLSHVQEGKPSPPFFPSLHNKNLQPKILASLEQKNGEIKPKSRRRWGLISRSTKGSDDWADLDDLDFSPSLTRIDVKNKKRQSDDTLCRFESVLDLKPSESVGTGTTVSTQASSQRRDTPTLQSSAKQHYLKHSRYLPGINIRNGVLPNPGKDFLPSNSWSSSGLSGKSSGTVSVVSKITSVGSGSASSSGLTGSYIPSFLKKEIGSVMQRVQLAPLAAPPPGYSSLKAVRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRIDWSSKYPSRR

Target ORF information:

RefSeq Version	XM_030244466.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus intestinal cell kinase (Ick), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244466.1

ORF Insert Sequence:

ATGAAGGAAA ACCTTTACCA GCTCATTAAA GAAAGAAATA AGTTGTTTCC AGAGTCTGCT 
ATAAGGAATA TAATGTACCA GATATTGCAA GGACTGGCAT TCATTCACAA ACACGGTTTC 
TTCCACCGGG ACTTAAAACC CGAGAACCTC CTCTGCATGG GACCAGAACT TGTGAAAATC 
GCCGACTTTG GATTGGCTCG AGAAATCCGA TCAAGACCTC CGTACACAGA CTATGTGTCT 
ACTAGATGGT ACAGGGCTCC AGAGGTACTG CTGAGATCCA CCAACTACAG CTCCCCCATT 
GACATCTGGG CGGTAGGCTG CATCATGGCG GAGGTGTACA CGCTCCGGCC CCTTTTCCCT 
GGGGCCAGTG AAATTGACAC AATTTTCAAA ATTTGCCAAG TGTTGGGAAC ACCAAAGAAG 
ACTGACTGGC CTGAAGGCTA CCAGCTGTCA AGTGCTATGA ATTTCCTCTG GCCCCAGTGC 
ATACCCAATA ACTTAAAGAC TCTAATCCCA AATGCTAGCA GCGAAGCCAT TCAGCTCCTG 
AGAGACTTGC TTCAGTGGGA TCCCAAGAAA CGACCAACCG CTAGTCAGGC TCTTCGATAT 
CCTTACTTCC AGATCGGACA CCCACTGGGC ATCATCAGCA AGGACTCAGG AAAACCACAG 
AGAGAGGTCC AGGACAAGAC AGGCCCTCCT CCTTACATCA AGCCAGCCCC TCCTGCCCAG 
GCCCCGGCCA AGGCATATAC GCTGATTTCT TCACGACCAA GCCAAGCCAG TCAGCCCCCT 
CAGCATTCTG TGCACCCCTA CAAAGGCGAT GTCTCTAGGA CTGAGCAGCT GAGCCATGTT 
CAGGAGGGCA AGCCAAGCCC TCCATTCTTC CCATCCCTCC ACAATAAGAA TCTTCAGCCA 
AAAATCCTAG CCAGCCTGGA ACAGAAAAAT GGTGAAATCA AGCCAAAGAG CAGGAGAAGA 
TGGGGTCTCA TCTCCAGGTC GACAAAGGGT TCTGACGACT GGGCCGACTT GGATGACTTA 
GACTTCAGTC CCTCTCTCAC CAGGATAGAT GTAAAAAACA AGAAGAGACA GAGTGATGAC 
ACCCTCTGCA GGTTTGAAAG TGTTCTGGAC CTGAAGCCAT CTGAGTCAGT GGGTACAGGA 
ACCACTGTCT CCACGCAGGC TTCCTCGCAG AGGAGAGACA CGCCGACCCT GCAGTCCTCA 
GCAAAGCAGC ACTACTTGAA GCACTCTCGC TACTTGCCTG GAATAAATAT AAGAAATGGC 
GTGCTCCCAA ATCCAGGCAA GGACTTTCTT CCATCAAATT CATGGTCCAG TTCCGGCTTG 
TCTGGAAAAT CTTCAGGAAC AGTATCAGTA GTGAGCAAAA TAACTTCAGT TGGCTCAGGC 
TCTGCGAGCT CTAGTGGACT GACTGGAAGC TACATTCCTT CCTTTCTGAA AAAGGAAATC 
GGTTCTGTCA TGCAGAGGGT TCAGCTGGCG CCCCTCGCAG CCCCTCCCCC CGGCTACTCT 
TCCCTCAAGG CCGTGAGACC TCACCCGGGG AGGCCTTTCT TTCACACCCA GCCCAGGAGC 
ACGCCCGGCT TGATCCCCCG ACCTCCAGCT GCTCAGCCTG TGCACGGCAG GATAGACTGG 
TCTTCCAAGT ACCCATCCCG GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Murine Model for Human ECO Syndrome Reveals a Critical Role of Intestinal Cell Kinase in Skeletal Development.
Calcified tissue international(2017 Nov)
Ding M,Jin L,Xie L,Park SH,Tong Y,Wu D,Chhabra AB,Fu Z,Li X

An essential role of intestinal cell kinase in lung development is linked to the perinatal lethality of human ECO syndrome.
FEBS letters591(9)1247-1257(2017 05)
Tong Y,Park SH,Wu D,Xu W,Guillot SJ,Jin L,Li X,Wang Y,Lin CS,Fu Z

Ick Ciliary Kinase Is Essential for Planar Cell Polarity Formation in Inner Ear Hair Cells and Hearing Function.
The Journal of neuroscience : the official journal of the Society for Neuroscience37(8)2073-2085(2017 Feb)
Okamoto S,Chaya T,Omori Y,Kuwahara R,Kubo S,Sakaguchi H,Furukawa T

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Cilk1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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