Mast1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mast1
Entrez Gene ID	56527
Full Name	microtubule associated serine/threonine kinase 1
Synonyms	9430008B02Rik,SAST,SAST170
General protein information	Preferred Names microtubule associated serine/threonine kinase 1 Names microtubule-associated serine/threonine-protein kinase 1 mast1 syntrophin associated serine/threonine kinase syntrophin-associated serine-threonine protein kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 C3\|8 41.32 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006531216.4	XP_006531279.1	microtubule-associated serine/threonine-protein kinase 1 isoform X1
XM_006531217.3	XP_006531280.1	microtubule-associated serine/threonine-protein kinase 1 isoform X2
XM_006531217.4	XP_006531280.1	microtubule-associated serine/threonine-protein kinase 1 isoform X2
XM_006531216.3	XP_006531279.1	microtubule-associated serine/threonine-protein kinase 1 isoform X1
NM_019945.2	NP_064329.2	microtubule-associated serine/threonine-protein kinase 1
XM_017312921.1	XP_017168410.1	microtubule-associated serine/threonine-protein kinase 1 isoform X3
XM_030243695.1	XP_030099555.1	microtubule-associated serine/threonine-protein kinase 1 isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MAST1	NP_001178457.1
Danio rerio (zebrafish)	LOC796144	XP_001336447.3
Xenopus tropicalis (tropical clawed frog)	mast1	XP_002938529.2
Pan troglodytes (chimpanzee)	MAST1	XP_003316199.2
Canis lupus familiaris (dog)	MAST1	XP_005633268.1
Danio rerio (zebrafish)	mast1a	XP_001337128.3
Rattus norvegicus (Norway rat)	MAST1	NP_851603.1
Homo sapiens (human)	MAST1	NP_055790.1
	MAST1	XP_001109914.2
Mus musculus (house mouse)	Mast1	NP_064329.2

Gene Ontology
Bibliography

Related articles in PubMed

Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases.
Valiente M, Andr?s-Pons A, Gomar B, Torres J, Gil A, Tapparel C, Antonarakis SE, Pulido R
The Journal of biological chemistry280(32)28936-43(2005 Aug)

Genomic analysis of mouse retinal development.
Blackshaw S, Harpavat S, Trimarchi J, Cai L, Huang H, Kuo WP, Weber G, Lee K, Fraioli RE, Cho SH, Yung R, Asch E, Ohno-Machado L, Wong WH, Cepko CL
PLoS biology2(9)E247(2004 Sep)

Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Aizawa H, Yuasa S, Nakajima D, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(1)35-48(2003 Feb)

Deoxyribonuclease II: structure and chromosomal localization of the murine gene, and comparison with the genomic structure of the human and three C. elegans homologs.
Krieser RJ, Eastman A
Gene252(1-2)155-62(2000 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mast1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mast1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu82548	XM_017312921.1 Latest version!	Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu82548	XM_030243695.1 Latest version!	Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu41630	XM_006531216.4 Latest version!	Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu41631	XM_006531217.4 Latest version!	Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu11725	NM_019945.2 Latest version!	Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu41631	XM_006531217.3	Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu41630	XM_006531216.3	Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mast1 ( XM_017312921.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168410.1 Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu82548
Clone ID Related Accession (Same CDS sequence)	XM_017312921.1 , XM_030243695.1
Accession Version	XM_017312921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACCAG CTACTTTGAC AGTGTACAGC AGTATGGAGC AGCTGTCTCA GCACGAGCCT 
AAGACCCCCG TCTCAGCATC TGGGGCCAGC AAGCGGGACC CAAGCGCTAA GGGCCCGGAG 
GAGAAAGTGG CCGGCAAGAG GGAGGGACTG GGTGGCCTGA CTCTGCGTGA GAAGACCTGG 
AGAGGGGGCT CTCCAGAGAT TAAGCGCTTC TCAGCGTCTG AAGCCAGTTT TCTGGAAGGA 
GAGGCCAGTC CCCCGCTGGG AGCGCGCAGA CGTTTCTCAG CACTGCTGGA GCCCAGCCGC 
TTCACTGCCC CTCAAGAGGA TGAAGATGAG GCTCGACTCC GCAGACCTCC TCGACCCAGC 
TCCGATCCCC CTAGCTCACT GGACACACGC GTCCCCAAAG AGGCAGTTCA AGGAGAGGGC 
ACGTCCACCC CCGGGGAACC CGAGGCCACT GAGCGCTCAC ATCCAGGTGA CCTCGGCCCA 
CCCTCCAAGG ATGGAGACCC ATCAGGCCCT AGGGCCACCA ACGACCTGGT TTTGCGAAGG 
GCAAGGCACC AGCAGCTGTC TGGGGATCTT GCTGTGGAGA AGCGACCGTC TCGAACTGGG 
GGCAAAGTTA TCAAGTCGGC TTCAGCTACA GCCTTGTCAG TCATGATTCC TGCAGTGGAC 
CCACATGGAG GCTCACCCCT GGCTAGTCCC ATGTCCCCTC GATCTCTCTC CTCCAATCCA 
TCCTCACGGG ACTCTTCGCC TAGCCGAGAC TACTCACCAG CTGTCAGTGG ACTCCGTTCC 
CCCATCACCA TCCAACGCTC AGGCAAGAAA TATGGGTTCA CACTGCGAGC CATCCGTGTC 
TACATGGGCG ACTCCGATGT CTACAGTGTC CACCACATTG TTTGGCACGT GGAGGAAGGA 
GGCCCAGCCC AAGAGGCAGG GCTCTGTGCT GGGGACCTCA TCACTCACGT GAATGGTGAG 
CCCGTGCATG GTATGGTGCA TCCTGAGGTG GTAGAGCTGA TCCTGAAGAG TGGCAACAAA 
GTAGCTGTGA CCACCACACC CTTTGAAAAC ACTTCCATCC GGATTGGGCC TGCCAGGCGC 
AGCAGTTACA AAGCCAAAAT GGCCCGAAGG AACAAAAGGC CTTCGGCCAA AGACGGCCAG 
GAGAGCAAGA AGCGGAGCTC TCTCTTTCGG AAGATCACAA AGCAGTCGAA CCTGCTGCAC 
ACCAGTCGAT CGCTGTCCTC TTTGAACCGG TCCTTGTCGT CCAGCGACAG TCTTCCGGGC 
TCGCCCACGC ACGGACTACC CGCACGCTCG CCCACACATA GCTATCGCTC TACACCTGAC 
TCCGCCTACC TAGGCGCCTC GTCACAGAGT AGCTCGCCAG CTTCCAGCAC GCCTAATTCC 
CCCGCCTCAT CCGCCTCGCA CCACATCAGG CCCAGCACCT TGCACGGGCT GTCACCGAAA 
CTACACCGCC AGTACCGCTC AGCGCGCTGT AAATCGGCTG GCAACATCCC GCTGTCACCT 
CTAGCGCACA CACCATCCCC GACGCAGGCG TCGCCGCCGC CGCTGCCAGG CCACACGGTG 
GGCAGCTCAC ATACCACGCA GAGCTTCCCT GCCAAACTAC ACTCATCGCC ACCTGTCGTG 
CGCCCGCGCC CCAAAAGTGC AGAGCCACCA CGCTCGCCGT TGCTCAAGCG CGTGCAGTCA 
GCGGAGAAAC TAGGGGCTTC GCTGGGAGCA GACAAGAAGG GCGCACTGCG CAAACACAGC 
CTGGAAGTGG GACACCCTGA TTTCCGAAAG GACTTCCATG GTGAGCTGGC CCTGCATAGC 
CTCGCTGAGT CTGATGGTGA GACGCCTCCT ATTGAGGGTC CTGGGGCGAC CCGGCAGGTC 
GCCGTGCGAC GCCTGGGTCG CCAGGAATCA CCCCTAAGCT TGGGCGCGGA CCCGTTGCTA 
CCCGATGGTG TCCAGAGACC TATGGCGTCA AGCAAGGAGG ATTCCGCGGG AGGTACAGAG 
GCTTGCACCC CACCTCGCGC GACAACCCCT GGGAGCCGGA CCCTGGAGCG TGACTCGGGT 
TGCACAAGGC ACCAGAGCGT GCAGACTGAG GATGGCCCAG GTGGGGTGGC CAGGGCCCTG 
GCCAAGGCAG CGCTAAGTCC AGTGCAGGAA CATGAGACAG GCCGGCGCAG TAGCTCTGGT 
GAGGCAGGCA CACCCCCGGT GCCCATTGTT GTAGAGCCTG CTCGGCCAGG GGTCAAGACC 
CAGGCACCCC AGCCTCTGGG CACAGACTCC AAGGGATTGA AGGAACCTGT AGCCCAGATG 
CCTCTGATGC CTGATGCCCC AAGGGGCAGG GAGCGCTGGG TGCTAGAGGA GGTGGAAGAG 
CGGACCACGC TAAGCGGACT TCGCTCTAAG CCCGCCTCCC CCAAACTCTC TCCTGACCCC 
CAGACTCCAA CCCTAGTCCC AACAAAGAAT GTACCCCGCA GTGCAGCTCC TTCAGTCCCA 
CCTGCTTCCC TCATGGTCCC AGGCACCAAG CCTGAAGCAG GGTTGAACTC CCGGTGTCCT 
GCTGAAGCTG TGACTCCTGC AGGCCTCACC AAAACTGGGG CACCTAGCCC TGCATCTCTA 
GGTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168410.1
CDS	64..2652
Translation	MTPATLTVYSSMEQLSQHEPKTPVSASGASKRDPSAKGPEEKVAGKREGLGGLTLREKTWRGGSPEIKRFSASEASFLEGEASPPLGARRRFSALLEPSRFTAPQEDEDEARLRRPPRPSSDPPSSLDTRVPKEAVQGEGTSTPGEPEATERSHPGDLGPPSKDGDPSGPRATNDLVLRRARHQQLSGDLAVEKRPSRTGGKVIKSASATALSVMIPAVDPHGGSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYGFTLRAIRVYMGDSDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVAVTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKDGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSLSSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASSQSSSPASSTPNSPASSASHHIRPSTLHGLSPKLHRQYRSARCKSAGNIPLSPLAHTPSPTQASPPPLPGHTVGSSHTTQSFPAKLHSSPPVVRPRPKSAEPPRSPLLKRVQSAEKLGASLGADKKGALRKHSLEVGHPDFRKDFHGELALHSLAESDGETPPIEGPGATRQVAVRRLGRQESPLSLGADPLLPDGVQRPMASSKEDSAGGTEACTPPRATTPGSRTLERDSGCTRHQSVQTEDGPGGVARALAKAALSPVQEHETGRRSSSGEAGTPPVPIVVEPARPGVKTQAPQPLGTDSKGLKEPVAQMPLMPDAPRGRERWVLEEVEERTTLSGLRSKPASPKLSPDPQTPTLVPTKNVPRSAAPSVPPASLMVPGTKPEAGLNSRCPAEAVTPAGLTKTGAPSPASLGP

Target ORF information:

RefSeq Version	XM_017312921.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312921.1

ORF Insert Sequence:

ATGACACCAG CTACTTTGAC AGTGTACAGC AGTATGGAGC AGCTGTCTCA GCACGAGCCT 
AAGACCCCCG TCTCAGCATC TGGGGCCAGC AAGCGGGACC CAAGCGCTAA GGGCCCGGAG 
GAGAAAGTGG CCGGCAAGAG GGAGGGACTG GGTGGCCTGA CTCTGCGTGA GAAGACCTGG 
AGAGGGGGCT CTCCAGAGAT TAAGCGCTTC TCAGCGTCTG AAGCCAGTTT TCTGGAAGGA 
GAGGCCAGTC CCCCGCTGGG AGCGCGCAGA CGTTTCTCAG CACTGCTGGA GCCCAGCCGC 
TTCACTGCCC CTCAAGAGGA TGAAGATGAG GCTCGACTCC GCAGACCTCC TCGACCCAGC 
TCCGATCCCC CTAGCTCACT GGACACACGC GTCCCCAAAG AGGCAGTTCA AGGAGAGGGC 
ACGTCCACCC CCGGGGAACC CGAGGCCACT GAGCGCTCAC ATCCAGGTGA CCTCGGCCCA 
CCCTCCAAGG ATGGAGACCC ATCAGGCCCT AGGGCCACCA ACGACCTGGT TTTGCGAAGG 
GCAAGGCACC AGCAGCTGTC TGGGGATCTT GCTGTGGAGA AGCGACCGTC TCGAACTGGG 
GGCAAAGTTA TCAAGTCGGC TTCAGCTACA GCCTTGTCAG TCATGATTCC TGCAGTGGAC 
CCACATGGAG GCTCACCCCT GGCTAGTCCC ATGTCCCCTC GATCTCTCTC CTCCAATCCA 
TCCTCACGGG ACTCTTCGCC TAGCCGAGAC TACTCACCAG CTGTCAGTGG ACTCCGTTCC 
CCCATCACCA TCCAACGCTC AGGCAAGAAA TATGGGTTCA CACTGCGAGC CATCCGTGTC 
TACATGGGCG ACTCCGATGT CTACAGTGTC CACCACATTG TTTGGCACGT GGAGGAAGGA 
GGCCCAGCCC AAGAGGCAGG GCTCTGTGCT GGGGACCTCA TCACTCACGT GAATGGTGAG 
CCCGTGCATG GTATGGTGCA TCCTGAGGTG GTAGAGCTGA TCCTGAAGAG TGGCAACAAA 
GTAGCTGTGA CCACCACACC CTTTGAAAAC ACTTCCATCC GGATTGGGCC TGCCAGGCGC 
AGCAGTTACA AAGCCAAAAT GGCCCGAAGG AACAAAAGGC CTTCGGCCAA AGACGGCCAG 
GAGAGCAAGA AGCGGAGCTC TCTCTTTCGG AAGATCACAA AGCAGTCGAA CCTGCTGCAC 
ACCAGTCGAT CGCTGTCCTC TTTGAACCGG TCCTTGTCGT CCAGCGACAG TCTTCCGGGC 
TCGCCCACGC ACGGACTACC CGCACGCTCG CCCACACATA GCTATCGCTC TACACCTGAC 
TCCGCCTACC TAGGCGCCTC GTCACAGAGT AGCTCGCCAG CTTCCAGCAC GCCTAATTCC 
CCCGCCTCAT CCGCCTCGCA CCACATCAGG CCCAGCACCT TGCACGGGCT GTCACCGAAA 
CTACACCGCC AGTACCGCTC AGCGCGCTGT AAATCGGCTG GCAACATCCC GCTGTCACCT 
CTAGCGCACA CACCATCCCC GACGCAGGCG TCGCCGCCGC CGCTGCCAGG CCACACGGTG 
GGCAGCTCAC ATACCACGCA GAGCTTCCCT GCCAAACTAC ACTCATCGCC ACCTGTCGTG 
CGCCCGCGCC CCAAAAGTGC AGAGCCACCA CGCTCGCCGT TGCTCAAGCG CGTGCAGTCA 
GCGGAGAAAC TAGGGGCTTC GCTGGGAGCA GACAAGAAGG GCGCACTGCG CAAACACAGC 
CTGGAAGTGG GACACCCTGA TTTCCGAAAG GACTTCCATG GTGAGCTGGC CCTGCATAGC 
CTCGCTGAGT CTGATGGTGA GACGCCTCCT ATTGAGGGTC CTGGGGCGAC CCGGCAGGTC 
GCCGTGCGAC GCCTGGGTCG CCAGGAATCA CCCCTAAGCT TGGGCGCGGA CCCGTTGCTA 
CCCGATGGTG TCCAGAGACC TATGGCGTCA AGCAAGGAGG ATTCCGCGGG AGGTACAGAG 
GCTTGCACCC CACCTCGCGC GACAACCCCT GGGAGCCGGA CCCTGGAGCG TGACTCGGGT 
TGCACAAGGC ACCAGAGCGT GCAGACTGAG GATGGCCCAG GTGGGGTGGC CAGGGCCCTG 
GCCAAGGCAG CGCTAAGTCC AGTGCAGGAA CATGAGACAG GCCGGCGCAG TAGCTCTGGT 
GAGGCAGGCA CACCCCCGGT GCCCATTGTT GTAGAGCCTG CTCGGCCAGG GGTCAAGACC 
CAGGCACCCC AGCCTCTGGG CACAGACTCC AAGGGATTGA AGGAACCTGT AGCCCAGATG 
CCTCTGATGC CTGATGCCCC AAGGGGCAGG GAGCGCTGGG TGCTAGAGGA GGTGGAAGAG 
CGGACCACGC TAAGCGGACT TCGCTCTAAG CCCGCCTCCC CCAAACTCTC TCCTGACCCC 
CAGACTCCAA CCCTAGTCCC AACAAAGAAT GTACCCCGCA GTGCAGCTCC TTCAGTCCCA 
CCTGCTTCCC TCATGGTCCC AGGCACCAAG CCTGAAGCAG GGTTGAACTC CCGGTGTCCT 
GCTGAAGCTG TGACTCCTGC AGGCCTCACC AAAACTGGGG CACCTAGCCC TGCATCTCTA 
GGTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast1 ( XM_030243695.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099555.1 Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu82548
Clone ID Related Accession (Same CDS sequence)	XM_017312921.1 , XM_030243695.1
Accession Version	XM_030243695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACCAG CTACTTTGAC AGTGTACAGC AGTATGGAGC AGCTGTCTCA GCACGAGCCT 
AAGACCCCCG TCTCAGCATC TGGGGCCAGC AAGCGGGACC CAAGCGCTAA GGGCCCGGAG 
GAGAAAGTGG CCGGCAAGAG GGAGGGACTG GGTGGCCTGA CTCTGCGTGA GAAGACCTGG 
AGAGGGGGCT CTCCAGAGAT TAAGCGCTTC TCAGCGTCTG AAGCCAGTTT TCTGGAAGGA 
GAGGCCAGTC CCCCGCTGGG AGCGCGCAGA CGTTTCTCAG CACTGCTGGA GCCCAGCCGC 
TTCACTGCCC CTCAAGAGGA TGAAGATGAG GCTCGACTCC GCAGACCTCC TCGACCCAGC 
TCCGATCCCC CTAGCTCACT GGACACACGC GTCCCCAAAG AGGCAGTTCA AGGAGAGGGC 
ACGTCCACCC CCGGGGAACC CGAGGCCACT GAGCGCTCAC ATCCAGGTGA CCTCGGCCCA 
CCCTCCAAGG ATGGAGACCC ATCAGGCCCT AGGGCCACCA ACGACCTGGT TTTGCGAAGG 
GCAAGGCACC AGCAGCTGTC TGGGGATCTT GCTGTGGAGA AGCGACCGTC TCGAACTGGG 
GGCAAAGTTA TCAAGTCGGC TTCAGCTACA GCCTTGTCAG TCATGATTCC TGCAGTGGAC 
CCACATGGAG GCTCACCCCT GGCTAGTCCC ATGTCCCCTC GATCTCTCTC CTCCAATCCA 
TCCTCACGGG ACTCTTCGCC TAGCCGAGAC TACTCACCAG CTGTCAGTGG ACTCCGTTCC 
CCCATCACCA TCCAACGCTC AGGCAAGAAA TATGGGTTCA CACTGCGAGC CATCCGTGTC 
TACATGGGCG ACTCCGATGT CTACAGTGTC CACCACATTG TTTGGCACGT GGAGGAAGGA 
GGCCCAGCCC AAGAGGCAGG GCTCTGTGCT GGGGACCTCA TCACTCACGT GAATGGTGAG 
CCCGTGCATG GTATGGTGCA TCCTGAGGTG GTAGAGCTGA TCCTGAAGAG TGGCAACAAA 
GTAGCTGTGA CCACCACACC CTTTGAAAAC ACTTCCATCC GGATTGGGCC TGCCAGGCGC 
AGCAGTTACA AAGCCAAAAT GGCCCGAAGG AACAAAAGGC CTTCGGCCAA AGACGGCCAG 
GAGAGCAAGA AGCGGAGCTC TCTCTTTCGG AAGATCACAA AGCAGTCGAA CCTGCTGCAC 
ACCAGTCGAT CGCTGTCCTC TTTGAACCGG TCCTTGTCGT CCAGCGACAG TCTTCCGGGC 
TCGCCCACGC ACGGACTACC CGCACGCTCG CCCACACATA GCTATCGCTC TACACCTGAC 
TCCGCCTACC TAGGCGCCTC GTCACAGAGT AGCTCGCCAG CTTCCAGCAC GCCTAATTCC 
CCCGCCTCAT CCGCCTCGCA CCACATCAGG CCCAGCACCT TGCACGGGCT GTCACCGAAA 
CTACACCGCC AGTACCGCTC AGCGCGCTGT AAATCGGCTG GCAACATCCC GCTGTCACCT 
CTAGCGCACA CACCATCCCC GACGCAGGCG TCGCCGCCGC CGCTGCCAGG CCACACGGTG 
GGCAGCTCAC ATACCACGCA GAGCTTCCCT GCCAAACTAC ACTCATCGCC ACCTGTCGTG 
CGCCCGCGCC CCAAAAGTGC AGAGCCACCA CGCTCGCCGT TGCTCAAGCG CGTGCAGTCA 
GCGGAGAAAC TAGGGGCTTC GCTGGGAGCA GACAAGAAGG GCGCACTGCG CAAACACAGC 
CTGGAAGTGG GACACCCTGA TTTCCGAAAG GACTTCCATG GTGAGCTGGC CCTGCATAGC 
CTCGCTGAGT CTGATGGTGA GACGCCTCCT ATTGAGGGTC CTGGGGCGAC CCGGCAGGTC 
GCCGTGCGAC GCCTGGGTCG CCAGGAATCA CCCCTAAGCT TGGGCGCGGA CCCGTTGCTA 
CCCGATGGTG TCCAGAGACC TATGGCGTCA AGCAAGGAGG ATTCCGCGGG AGGTACAGAG 
GCTTGCACCC CACCTCGCGC GACAACCCCT GGGAGCCGGA CCCTGGAGCG TGACTCGGGT 
TGCACAAGGC ACCAGAGCGT GCAGACTGAG GATGGCCCAG GTGGGGTGGC CAGGGCCCTG 
GCCAAGGCAG CGCTAAGTCC AGTGCAGGAA CATGAGACAG GCCGGCGCAG TAGCTCTGGT 
GAGGCAGGCA CACCCCCGGT GCCCATTGTT GTAGAGCCTG CTCGGCCAGG GGTCAAGACC 
CAGGCACCCC AGCCTCTGGG CACAGACTCC AAGGGATTGA AGGAACCTGT AGCCCAGATG 
CCTCTGATGC CTGATGCCCC AAGGGGCAGG GAGCGCTGGG TGCTAGAGGA GGTGGAAGAG 
CGGACCACGC TAAGCGGACT TCGCTCTAAG CCCGCCTCCC CCAAACTCTC TCCTGACCCC 
CAGACTCCAA CCCTAGTCCC AACAAAGAAT GTACCCCGCA GTGCAGCTCC TTCAGTCCCA 
CCTGCTTCCC TCATGGTCCC AGGCACCAAG CCTGAAGCAG GGTTGAACTC CCGGTGTCCT 
GCTGAAGCTG TGACTCCTGC AGGCCTCACC AAAACTGGGG CACCTAGCCC TGCATCTCTA 
GGTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099555.1
CDS	1241..3829
Translation	MTPATLTVYSSMEQLSQHEPKTPVSASGASKRDPSAKGPEEKVAGKREGLGGLTLREKTWRGGSPEIKRFSASEASFLEGEASPPLGARRRFSALLEPSRFTAPQEDEDEARLRRPPRPSSDPPSSLDTRVPKEAVQGEGTSTPGEPEATERSHPGDLGPPSKDGDPSGPRATNDLVLRRARHQQLSGDLAVEKRPSRTGGKVIKSASATALSVMIPAVDPHGGSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYGFTLRAIRVYMGDSDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVAVTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKDGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSLSSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASSQSSSPASSTPNSPASSASHHIRPSTLHGLSPKLHRQYRSARCKSAGNIPLSPLAHTPSPTQASPPPLPGHTVGSSHTTQSFPAKLHSSPPVVRPRPKSAEPPRSPLLKRVQSAEKLGASLGADKKGALRKHSLEVGHPDFRKDFHGELALHSLAESDGETPPIEGPGATRQVAVRRLGRQESPLSLGADPLLPDGVQRPMASSKEDSAGGTEACTPPRATTPGSRTLERDSGCTRHQSVQTEDGPGGVARALAKAALSPVQEHETGRRSSSGEAGTPPVPIVVEPARPGVKTQAPQPLGTDSKGLKEPVAQMPLMPDAPRGRERWVLEEVEERTTLSGLRSKPASPKLSPDPQTPTLVPTKNVPRSAAPSVPPASLMVPGTKPEAGLNSRCPAEAVTPAGLTKTGAPSPASLGP

Target ORF information:

RefSeq Version	XM_030243695.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243695.1

ORF Insert Sequence:

ATGACACCAG CTACTTTGAC AGTGTACAGC AGTATGGAGC AGCTGTCTCA GCACGAGCCT 
AAGACCCCCG TCTCAGCATC TGGGGCCAGC AAGCGGGACC CAAGCGCTAA GGGCCCGGAG 
GAGAAAGTGG CCGGCAAGAG GGAGGGACTG GGTGGCCTGA CTCTGCGTGA GAAGACCTGG 
AGAGGGGGCT CTCCAGAGAT TAAGCGCTTC TCAGCGTCTG AAGCCAGTTT TCTGGAAGGA 
GAGGCCAGTC CCCCGCTGGG AGCGCGCAGA CGTTTCTCAG CACTGCTGGA GCCCAGCCGC 
TTCACTGCCC CTCAAGAGGA TGAAGATGAG GCTCGACTCC GCAGACCTCC TCGACCCAGC 
TCCGATCCCC CTAGCTCACT GGACACACGC GTCCCCAAAG AGGCAGTTCA AGGAGAGGGC 
ACGTCCACCC CCGGGGAACC CGAGGCCACT GAGCGCTCAC ATCCAGGTGA CCTCGGCCCA 
CCCTCCAAGG ATGGAGACCC ATCAGGCCCT AGGGCCACCA ACGACCTGGT TTTGCGAAGG 
GCAAGGCACC AGCAGCTGTC TGGGGATCTT GCTGTGGAGA AGCGACCGTC TCGAACTGGG 
GGCAAAGTTA TCAAGTCGGC TTCAGCTACA GCCTTGTCAG TCATGATTCC TGCAGTGGAC 
CCACATGGAG GCTCACCCCT GGCTAGTCCC ATGTCCCCTC GATCTCTCTC CTCCAATCCA 
TCCTCACGGG ACTCTTCGCC TAGCCGAGAC TACTCACCAG CTGTCAGTGG ACTCCGTTCC 
CCCATCACCA TCCAACGCTC AGGCAAGAAA TATGGGTTCA CACTGCGAGC CATCCGTGTC 
TACATGGGCG ACTCCGATGT CTACAGTGTC CACCACATTG TTTGGCACGT GGAGGAAGGA 
GGCCCAGCCC AAGAGGCAGG GCTCTGTGCT GGGGACCTCA TCACTCACGT GAATGGTGAG 
CCCGTGCATG GTATGGTGCA TCCTGAGGTG GTAGAGCTGA TCCTGAAGAG TGGCAACAAA 
GTAGCTGTGA CCACCACACC CTTTGAAAAC ACTTCCATCC GGATTGGGCC TGCCAGGCGC 
AGCAGTTACA AAGCCAAAAT GGCCCGAAGG AACAAAAGGC CTTCGGCCAA AGACGGCCAG 
GAGAGCAAGA AGCGGAGCTC TCTCTTTCGG AAGATCACAA AGCAGTCGAA CCTGCTGCAC 
ACCAGTCGAT CGCTGTCCTC TTTGAACCGG TCCTTGTCGT CCAGCGACAG TCTTCCGGGC 
TCGCCCACGC ACGGACTACC CGCACGCTCG CCCACACATA GCTATCGCTC TACACCTGAC 
TCCGCCTACC TAGGCGCCTC GTCACAGAGT AGCTCGCCAG CTTCCAGCAC GCCTAATTCC 
CCCGCCTCAT CCGCCTCGCA CCACATCAGG CCCAGCACCT TGCACGGGCT GTCACCGAAA 
CTACACCGCC AGTACCGCTC AGCGCGCTGT AAATCGGCTG GCAACATCCC GCTGTCACCT 
CTAGCGCACA CACCATCCCC GACGCAGGCG TCGCCGCCGC CGCTGCCAGG CCACACGGTG 
GGCAGCTCAC ATACCACGCA GAGCTTCCCT GCCAAACTAC ACTCATCGCC ACCTGTCGTG 
CGCCCGCGCC CCAAAAGTGC AGAGCCACCA CGCTCGCCGT TGCTCAAGCG CGTGCAGTCA 
GCGGAGAAAC TAGGGGCTTC GCTGGGAGCA GACAAGAAGG GCGCACTGCG CAAACACAGC 
CTGGAAGTGG GACACCCTGA TTTCCGAAAG GACTTCCATG GTGAGCTGGC CCTGCATAGC 
CTCGCTGAGT CTGATGGTGA GACGCCTCCT ATTGAGGGTC CTGGGGCGAC CCGGCAGGTC 
GCCGTGCGAC GCCTGGGTCG CCAGGAATCA CCCCTAAGCT TGGGCGCGGA CCCGTTGCTA 
CCCGATGGTG TCCAGAGACC TATGGCGTCA AGCAAGGAGG ATTCCGCGGG AGGTACAGAG 
GCTTGCACCC CACCTCGCGC GACAACCCCT GGGAGCCGGA CCCTGGAGCG TGACTCGGGT 
TGCACAAGGC ACCAGAGCGT GCAGACTGAG GATGGCCCAG GTGGGGTGGC CAGGGCCCTG 
GCCAAGGCAG CGCTAAGTCC AGTGCAGGAA CATGAGACAG GCCGGCGCAG TAGCTCTGGT 
GAGGCAGGCA CACCCCCGGT GCCCATTGTT GTAGAGCCTG CTCGGCCAGG GGTCAAGACC 
CAGGCACCCC AGCCTCTGGG CACAGACTCC AAGGGATTGA AGGAACCTGT AGCCCAGATG 
CCTCTGATGC CTGATGCCCC AAGGGGCAGG GAGCGCTGGG TGCTAGAGGA GGTGGAAGAG 
CGGACCACGC TAAGCGGACT TCGCTCTAAG CCCGCCTCCC CCAAACTCTC TCCTGACCCC 
CAGACTCCAA CCCTAGTCCC AACAAAGAAT GTACCCCGCA GTGCAGCTCC TTCAGTCCCA 
CCTGCTTCCC TCATGGTCCC AGGCACCAAG CCTGAAGCAG GGTTGAACTC CCGGTGTCCT 
GCTGAAGCTG TGACTCCTGC AGGCCTCACC AAAACTGGGG CACCTAGCCC TGCATCTCTA 
GGTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast1 ( XM_006531216.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531279.1 Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X1, mRNA.

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CloneID	OMu41630
Clone ID Related Accession (Same CDS sequence)	XM_006531216.4 , XM_006531216.3
Accession Version	XM_006531216.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006531216.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGATT CTGGCCTGAT CCGCCGCCGG AGGCTGCAGA AGGATTTACC CCTGCCCCGG 
AAAAGCAGCA GTTGCCGTAC CAGCAACCGG AAAAGCCTCA TCCTGACAAG CACTTCTCCG 
ACTCTACCGC GACCCCACTC CCCGCTGCCG GGCCACTTAG GTAGCAGTCC TTTGGACAGC 
CCCCGCAACT TCTCTCCCAA CACTCCCGCC CACTTCTCGT TTGCTTCTTC CCGAAGGGCG 
GATGGACGCC GATGGTCGCT TGCTTCGCTC CCTTCCTCTG GCTATGGCAC CAACACGCCC 
AGCTCAACTG TTTCATCCTC CTGCTCCTCC CAGGAGCGCC TGCATCAACT ACCGTACCAG 
CCCACCGTGG ACGAGCTGCA CTTTCTGTCC AAACACTTCG GCAGCACCGA GAGCATCACC 
GACGAGGATG GTGGCCGCCG CTCTCCCGCC GTGCGTCCTC GCTCTCGCAG CCTCAGCCCC 
GGCCGCTCCC CCTCCTCCTA CGACAACGAA ATTGTGATGA TGAACCATGT GTACAAGGAG 
CGGTTCCCAA AGGCCACTGC ACAGATGGAA GAGAAGCTGC GGGACTTCGC CCGCGCCTAT 
GAACCTGACA GCGTGCTGCC GCTGGCCGAC GGCGTGCTCA GCTTCATCCA CCATCAGATC 
ATTGAACTGG CCCGAGACTG CCTTACCAAG TCCCGCGATG GGCTCATCAC CACGGTCTAT 
TTCTATGAAC TGCAGGAAAA CCTAGAGAAG CTGCTGCAGG ACGCTTATGA ACGCTCGGAG 
AGCTTGGAGG TAGCCTTTGT CACTCAGCTG GTGAAGAAAC TTCTCATAAT CATCTCTCGC 
CCTGCAAGGC TTCTGGAGTG CCTGGAATTC AACCCAGAAG AGTTCTACCA CCTTCTGGAG 
GCAGCCGAGG GACATGCCAA GGAGGGCCAC CTGGTCAAAA CTGACATCCC CCGATACATC 
ATCCGACAGC TGGGCCTCAC TCGGGACCCC TTTCCAGATG TGGTGCGCCT GGAGGAGCAG 
GACAGCGGGG GCTCCAACAC TCCGGAGCAA GACGACACAT CTGAGGGTCG CAGCAGCACC 
TCCAAGGCCA AGAAACCTCC CGGTGAAAGT GACTTTGACA CTATCAAGCT CATTAGCAAT 
GGTGCCTACG GAGCCGTCTA CCTGGTGCGG CACCGGGACA CGAGGCAGCG CTTCGCCATG 
AAGAAGATCA ACAAACAGAA CCTGATCCTG CGCAACCAGA TCCAGCAGGC GTTTGTGGAG 
CGCGACATCC TCACGTTTGC AGAGAACCCA TTTGTGGTCG GCATGTTCTG CTCCTTTGAG 
ACCCGGCGCC ACCTTTGCAT GGTCATGGAG TATGTGGAAG GTGGTGACTG TGCCACTCTC 
CTCAAGAACA TTGGGGCACT GCCTGTGGAG ATGGCGCGAA TGTATTTTGC AGAAACAGTT 
CTAGCCCTTG AGTACTTACA CAATTATGGC ATCGTGCACC GTGACCTCAA GCCAGACAAC 
CTCCTCATTA CTTCCATGGG CCACATCAAG CTCACGGATT TTGGCCTCTC CAAGATGGGA 
CTCATGAGCC TGACCACCAA CTTATATGAG GGGCACATTG AGAAGGATGC CCGGGAGTTC 
CTGGACAAAC AGGTGTGTGG GACCCCAGAG TACATCGCAC CCGAGGTCAT CCTGCGCCAG 
GGCTACGGCA AGCCAGTGGA CTGGTGGGCT ATGGGGATCA TTCTCTACGA GTTCCTGGTG 
GGCTGTGTGC CTTTCTTCGG GGATACCCCA GAAGAGCTCT TTGGACAGGT CATCAGCGAT 
GACATACTGT GGCCTGAAGG GGACGAGGCC CTGCCCACAG ATGCACAGCT TCTAATATCT 
AGCCTCTTGC AGACCAACCC CCTGGTCCGG CTTGGGGCAG GTGGTGCTTT TGAGGTGAAG 
CAGCATAGTT TCTTCCGAGA CCTGGACTGG ACAGGGCTAC TGCGGCAGAA GGCGGAGTTC 
ATCCCCCACC TAGAGTCAGA AGATGACACC AGCTACTTTG ACACCCGCTC CGACAGGTAT 
CACCATGTGA ACTCCTATGA TGAGGACGAC ACCACCGAAG AGGAGCCCGT GGAGATCCGC 
CAATTCTCCT CCTGCTCGCC GCGCTTCAGC AAGGTGTACA GCAGTATGGA GCAGCTGTCT 
CAGCACGAGC CTAAGACCCC CGTCTCAGCA TCTGGGGCCA GCAAGCGGGA CCCAAGCGCT 
AAGGGCCCGG AGGAGAAAGT GGCCGGCAAG AGGGAGGGAC TGGGTGGCCT GACTCTGCGT 
GAGAAGACCT GGAGAGGGGG CTCTCCAGAG ATTAAGCGCT TCTCAGCGTC TGAAGCCAGT 
TTTCTGGAAG GAGAGGCCAG TCCCCCGCTG GGAGCGCGCA GACGTTTCTC AGCACTGCTG 
GAGCCCAGCC GCTTCACTGC CCCTCAAGAG GATGAAGATG AGGCTCGACT CCGCAGACCT 
CCTCGACCCA GCTCCGATCC CCCTAGCTCA CTGGACACAC GCGTCCCCAA AGAGGCAGTT 
CAAGGAGAGG GCACGTCCAC CCCCGGGGAA CCCGAGGCCA CTGAGCGCTC ACATCCAGGT 
GACCTCGGCC CACCCTCCAA GGATGGAGAC CCATCAGGCC CTAGGGCCAC CAACGACCTG 
GTTTTGCGAA GGGCAAGGCA CCAGCAGCTG TCTGGGGATC TTGCTGTGGA GAAGCGACCG 
TCTCGAACTG GGGGCAAAGT TATCAAGTCG GCTTCAGCTA CAGCCTTGTC AGTCATGATT 
CCTGCAGTGG ACCCACATGG AGGCTCACCC CTGGCTAGTC CCATGTCCCC TCGATCTCTC 
TCCTCCAATC CATCCTCACG GGACTCTTCG CCTAGCCGAG ACTACTCACC AGCTGTCAGT 
GGACTCCGTT CCCCCATCAC CATCCAACGC TCAGGCAAGA AATATGGGTT CACACTGCGA 
GCCATCCGTG TCTACATGGG CGACTCCGAT GTCTACAGTG TCCACCACAT TGTTTGGCAC 
GTGGAGGAAG GAGGCCCAGC CCAAGAGGCA GGGCTCTGTG CTGGGGACCT CATCACTCAC 
GTGAATGGTG AGCCCGTGCA TGGTATGGTG CATCCTGAGG TGGTAGAGCT GATCCTGAAG 
AGTGGCAACA AAGTAGCTGT GACCACCACA CCCTTTGAAA ACACTTCCAT CCGGATTGGG 
CCTGCCAGGC GCAGCAGTTA CAAAGCCAAA ATGGCCCGAA GGAACAAAAG GCCTTCGGCC 
AAAGACGGCC AGGAGAGCAA GAAGCGGAGC TCTCTCTTTC GGAAGATCAC AAAGCAGTCG 
AACCTGCTGC ACACCAGTCG ATCGCTGTCC TCTTTGAACC GGTCCTTGTC GTCCAGCGAC 
AGTCTTCCGG GCTCGCCCAC GCACGGACTA CCCGCACGCT CGCCCACACA TAGCTATCGC 
TCTACACCTG ACTCCGCCTA CCTAGGCGCC TCGTCACAGA GTAGCTCGCC AGCTTCCAGC 
ACGCCTAATT CCCCCGCCTC ATCCGCCTCG CACCACATCA GGCCCAGCAC CTTGCACGGG 
CTGTCACCGA AACTACACCG CCAGTACCGC TCAGCGCGCT GTAAATCGGC TGGCAACATC 
CCGCTGTCAC CTCTAGCGCA CACACCATCC CCGACGCAGG CGTCGCCGCC GCCGCTGCCA 
GGCCACACGG TGGGCAGCTC ACATACCACG CAGAGCTTCC CTGCCAAACT ACACTCATCG 
CCACCTGTCG TGCGCCCGCG CCCCAAAAGT GCAGAGCCAC CACGCTCGCC GTTGCTCAAG 
CGCGTGCAGT CAGCGGAGAA ACTAGGGGCT TCGCTGGGAG CAGACAAGAA GGGCGCACTG 
CGCAAACACA GCCTGGAAGT GGGACACCCT GATTTCCGAA AGGACTTCCA TGGTGAGCTG 
GCCCTGCATA GCCTCGCTGA GTCTGATGGT GAGACGCCTC CTATTGAGGG TCCTGGGGCG 
ACCCGGCAGG TCGCCGTGCG ACGCCTGGGT CGCCAGGAAT CACCCCTAAG CTTGGGCGCG 
GACCCGTTGC TACCCGATGG TGTCCAGAGA CCTATGGCGT CAAGCAAGGA GGATTCCGCG 
GGAGGTACAG AGGCTTGCAC CCCACCTCGC GCGACAACCC CTGGGAGCCG GACCCTGGAG 
CGTGACTCGG GTTGCACAAG GCACCAGAGC GTGCAGACTG AGGATGGCCC AGGTGGGGTG 
GCCAGGGCCC TGGCCAAGGC AGCGCTAAGT CCAGTGCAGG AACATGAGAC AGGCCGGCGC 
AGTAGCTCTG GTGAGGCAGG CACACCCCCG GTGCCCATTG TTGTAGAGCC TGCTCGGCCA 
GGGGTCAAGA CCCAGGCACC CCAGCCTCTG GGCACAGACT CCAAGGGATT GAAGGAACCT 
GTAGCCCAGA TGCCTCTGAT GCCTGATGCC CCAAGGGGCA GGGAGCGCTG GGTGCTAGAG 
GAGGTGGAAG AGCGGACCAC GCTAAGCGGA CTTCGCTCTA AGCCCGCCTC CCCCAAACTC 
TCTCCTGACC CCCAGACTCC AACCCTAGTC CCAACAAAGA ATGTACCCCG CAGTGCAGCT 
CCTTCAGTCC CACCTGCTTC CCTCATGGTC CCAGGCACCA AGCCTGAAGC AGGGTTGAAC 
TCCCGGTGTC CTGCTGAAGC TGTGACTCCT GCAGGCCTCA CCAAAACTGG GGCACCTAGC 
CCTGCATCTC TAGGTCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531279.1
CDS	110..4810
Translation	MDDSGLIRRRRLQKDLPLPRKSSSCRTSNRKSLILTSTSPTLPRPHSPLPGHLGSSPLDSPRNFSPNTPAHFSFASSRRADGRRWSLASLPSSGYGTNTPSSTVSSSCSSQERLHQLPYQPTVDELHFLSKHFGSTESITDEDGGRRSPAVRPRSRSLSPGRSPSSYDNEIVMMNHVYKERFPKATAQMEEKLRDFARAYEPDSVLPLADGVLSFIHHQIIELARDCLTKSRDGLITTVYFYELQENLEKLLQDAYERSESLEVAFVTQLVKKLLIIISRPARLLECLEFNPEEFYHLLEAAEGHAKEGHLVKTDIPRYIIRQLGLTRDPFPDVVRLEEQDSGGSNTPEQDDTSEGRSSTSKAKKPPGESDFDTIKLISNGAYGAVYLVRHRDTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNIGALPVEMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDDILWPEGDEALPTDAQLLISSLLQTNPLVRLGAGGAFEVKQHSFFRDLDWTGLLRQKAEFIPHLESEDDTSYFDTRSDRYHHVNSYDEDDTTEEEPVEIRQFSSCSPRFSKVYSSMEQLSQHEPKTPVSASGASKRDPSAKGPEEKVAGKREGLGGLTLREKTWRGGSPEIKRFSASEASFLEGEASPPLGARRRFSALLEPSRFTAPQEDEDEARLRRPPRPSSDPPSSLDTRVPKEAVQGEGTSTPGEPEATERSHPGDLGPPSKDGDPSGPRATNDLVLRRARHQQLSGDLAVEKRPSRTGGKVIKSASATALSVMIPAVDPHGGSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYGFTLRAIRVYMGDSDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVAVTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKDGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSLSSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASSQSSSPASSTPNSPASSASHHIRPSTLHGLSPKLHRQYRSARCKSAGNIPLSPLAHTPSPTQASPPPLPGHTVGSSHTTQSFPAKLHSSPPVVRPRPKSAEPPRSPLLKRVQSAEKLGASLGADKKGALRKHSLEVGHPDFRKDFHGELALHSLAESDGETPPIEGPGATRQVAVRRLGRQESPLSLGADPLLPDGVQRPMASSKEDSAGGTEACTPPRATTPGSRTLERDSGCTRHQSVQTEDGPGGVARALAKAALSPVQEHETGRRSSSGEAGTPPVPIVVEPARPGVKTQAPQPLGTDSKGLKEPVAQMPLMPDAPRGRERWVLEEVEERTTLSGLRSKPASPKLSPDPQTPTLVPTKNVPRSAAPSVPPASLMVPGTKPEAGLNSRCPAEAVTPAGLTKTGAPSPASLGP

Target ORF information:

RefSeq Version	XM_006531216.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531216.4

ORF Insert Sequence:

ATGGACGATT CTGGCCTGAT CCGCCGCCGG AGGCTGCAGA AGGATTTACC CCTGCCCCGG 
AAAAGCAGCA GTTGCCGTAC CAGCAACCGG AAAAGCCTCA TCCTGACAAG CACTTCTCCG 
ACTCTACCGC GACCCCACTC CCCGCTGCCG GGCCACTTAG GTAGCAGTCC TTTGGACAGC 
CCCCGCAACT TCTCTCCCAA CACTCCCGCC CACTTCTCGT TTGCTTCTTC CCGAAGGGCG 
GATGGACGCC GATGGTCGCT TGCTTCGCTC CCTTCCTCTG GCTATGGCAC CAACACGCCC 
AGCTCAACTG TTTCATCCTC CTGCTCCTCC CAGGAGCGCC TGCATCAACT ACCGTACCAG 
CCCACCGTGG ACGAGCTGCA CTTTCTGTCC AAACACTTCG GCAGCACCGA GAGCATCACC 
GACGAGGATG GTGGCCGCCG CTCTCCCGCC GTGCGTCCTC GCTCTCGCAG CCTCAGCCCC 
GGCCGCTCCC CCTCCTCCTA CGACAACGAA ATTGTGATGA TGAACCATGT GTACAAGGAG 
CGGTTCCCAA AGGCCACTGC ACAGATGGAA GAGAAGCTGC GGGACTTCGC CCGCGCCTAT 
GAACCTGACA GCGTGCTGCC GCTGGCCGAC GGCGTGCTCA GCTTCATCCA CCATCAGATC 
ATTGAACTGG CCCGAGACTG CCTTACCAAG TCCCGCGATG GGCTCATCAC CACGGTCTAT 
TTCTATGAAC TGCAGGAAAA CCTAGAGAAG CTGCTGCAGG ACGCTTATGA ACGCTCGGAG 
AGCTTGGAGG TAGCCTTTGT CACTCAGCTG GTGAAGAAAC TTCTCATAAT CATCTCTCGC 
CCTGCAAGGC TTCTGGAGTG CCTGGAATTC AACCCAGAAG AGTTCTACCA CCTTCTGGAG 
GCAGCCGAGG GACATGCCAA GGAGGGCCAC CTGGTCAAAA CTGACATCCC CCGATACATC 
ATCCGACAGC TGGGCCTCAC TCGGGACCCC TTTCCAGATG TGGTGCGCCT GGAGGAGCAG 
GACAGCGGGG GCTCCAACAC TCCGGAGCAA GACGACACAT CTGAGGGTCG CAGCAGCACC 
TCCAAGGCCA AGAAACCTCC CGGTGAAAGT GACTTTGACA CTATCAAGCT CATTAGCAAT 
GGTGCCTACG GAGCCGTCTA CCTGGTGCGG CACCGGGACA CGAGGCAGCG CTTCGCCATG 
AAGAAGATCA ACAAACAGAA CCTGATCCTG CGCAACCAGA TCCAGCAGGC GTTTGTGGAG 
CGCGACATCC TCACGTTTGC AGAGAACCCA TTTGTGGTCG GCATGTTCTG CTCCTTTGAG 
ACCCGGCGCC ACCTTTGCAT GGTCATGGAG TATGTGGAAG GTGGTGACTG TGCCACTCTC 
CTCAAGAACA TTGGGGCACT GCCTGTGGAG ATGGCGCGAA TGTATTTTGC AGAAACAGTT 
CTAGCCCTTG AGTACTTACA CAATTATGGC ATCGTGCACC GTGACCTCAA GCCAGACAAC 
CTCCTCATTA CTTCCATGGG CCACATCAAG CTCACGGATT TTGGCCTCTC CAAGATGGGA 
CTCATGAGCC TGACCACCAA CTTATATGAG GGGCACATTG AGAAGGATGC CCGGGAGTTC 
CTGGACAAAC AGGTGTGTGG GACCCCAGAG TACATCGCAC CCGAGGTCAT CCTGCGCCAG 
GGCTACGGCA AGCCAGTGGA CTGGTGGGCT ATGGGGATCA TTCTCTACGA GTTCCTGGTG 
GGCTGTGTGC CTTTCTTCGG GGATACCCCA GAAGAGCTCT TTGGACAGGT CATCAGCGAT 
GACATACTGT GGCCTGAAGG GGACGAGGCC CTGCCCACAG ATGCACAGCT TCTAATATCT 
AGCCTCTTGC AGACCAACCC CCTGGTCCGG CTTGGGGCAG GTGGTGCTTT TGAGGTGAAG 
CAGCATAGTT TCTTCCGAGA CCTGGACTGG ACAGGGCTAC TGCGGCAGAA GGCGGAGTTC 
ATCCCCCACC TAGAGTCAGA AGATGACACC AGCTACTTTG ACACCCGCTC CGACAGGTAT 
CACCATGTGA ACTCCTATGA TGAGGACGAC ACCACCGAAG AGGAGCCCGT GGAGATCCGC 
CAATTCTCCT CCTGCTCGCC GCGCTTCAGC AAGGTGTACA GCAGTATGGA GCAGCTGTCT 
CAGCACGAGC CTAAGACCCC CGTCTCAGCA TCTGGGGCCA GCAAGCGGGA CCCAAGCGCT 
AAGGGCCCGG AGGAGAAAGT GGCCGGCAAG AGGGAGGGAC TGGGTGGCCT GACTCTGCGT 
GAGAAGACCT GGAGAGGGGG CTCTCCAGAG ATTAAGCGCT TCTCAGCGTC TGAAGCCAGT 
TTTCTGGAAG GAGAGGCCAG TCCCCCGCTG GGAGCGCGCA GACGTTTCTC AGCACTGCTG 
GAGCCCAGCC GCTTCACTGC CCCTCAAGAG GATGAAGATG AGGCTCGACT CCGCAGACCT 
CCTCGACCCA GCTCCGATCC CCCTAGCTCA CTGGACACAC GCGTCCCCAA AGAGGCAGTT 
CAAGGAGAGG GCACGTCCAC CCCCGGGGAA CCCGAGGCCA CTGAGCGCTC ACATCCAGGT 
GACCTCGGCC CACCCTCCAA GGATGGAGAC CCATCAGGCC CTAGGGCCAC CAACGACCTG 
GTTTTGCGAA GGGCAAGGCA CCAGCAGCTG TCTGGGGATC TTGCTGTGGA GAAGCGACCG 
TCTCGAACTG GGGGCAAAGT TATCAAGTCG GCTTCAGCTA CAGCCTTGTC AGTCATGATT 
CCTGCAGTGG ACCCACATGG AGGCTCACCC CTGGCTAGTC CCATGTCCCC TCGATCTCTC 
TCCTCCAATC CATCCTCACG GGACTCTTCG CCTAGCCGAG ACTACTCACC AGCTGTCAGT 
GGACTCCGTT CCCCCATCAC CATCCAACGC TCAGGCAAGA AATATGGGTT CACACTGCGA 
GCCATCCGTG TCTACATGGG CGACTCCGAT GTCTACAGTG TCCACCACAT TGTTTGGCAC 
GTGGAGGAAG GAGGCCCAGC CCAAGAGGCA GGGCTCTGTG CTGGGGACCT CATCACTCAC 
GTGAATGGTG AGCCCGTGCA TGGTATGGTG CATCCTGAGG TGGTAGAGCT GATCCTGAAG 
AGTGGCAACA AAGTAGCTGT GACCACCACA CCCTTTGAAA ACACTTCCAT CCGGATTGGG 
CCTGCCAGGC GCAGCAGTTA CAAAGCCAAA ATGGCCCGAA GGAACAAAAG GCCTTCGGCC 
AAAGACGGCC AGGAGAGCAA GAAGCGGAGC TCTCTCTTTC GGAAGATCAC AAAGCAGTCG 
AACCTGCTGC ACACCAGTCG ATCGCTGTCC TCTTTGAACC GGTCCTTGTC GTCCAGCGAC 
AGTCTTCCGG GCTCGCCCAC GCACGGACTA CCCGCACGCT CGCCCACACA TAGCTATCGC 
TCTACACCTG ACTCCGCCTA CCTAGGCGCC TCGTCACAGA GTAGCTCGCC AGCTTCCAGC 
ACGCCTAATT CCCCCGCCTC ATCCGCCTCG CACCACATCA GGCCCAGCAC CTTGCACGGG 
CTGTCACCGA AACTACACCG CCAGTACCGC TCAGCGCGCT GTAAATCGGC TGGCAACATC 
CCGCTGTCAC CTCTAGCGCA CACACCATCC CCGACGCAGG CGTCGCCGCC GCCGCTGCCA 
GGCCACACGG TGGGCAGCTC ACATACCACG CAGAGCTTCC CTGCCAAACT ACACTCATCG 
CCACCTGTCG TGCGCCCGCG CCCCAAAAGT GCAGAGCCAC CACGCTCGCC GTTGCTCAAG 
CGCGTGCAGT CAGCGGAGAA ACTAGGGGCT TCGCTGGGAG CAGACAAGAA GGGCGCACTG 
CGCAAACACA GCCTGGAAGT GGGACACCCT GATTTCCGAA AGGACTTCCA TGGTGAGCTG 
GCCCTGCATA GCCTCGCTGA GTCTGATGGT GAGACGCCTC CTATTGAGGG TCCTGGGGCG 
ACCCGGCAGG TCGCCGTGCG ACGCCTGGGT CGCCAGGAAT CACCCCTAAG CTTGGGCGCG 
GACCCGTTGC TACCCGATGG TGTCCAGAGA CCTATGGCGT CAAGCAAGGA GGATTCCGCG 
GGAGGTACAG AGGCTTGCAC CCCACCTCGC GCGACAACCC CTGGGAGCCG GACCCTGGAG 
CGTGACTCGG GTTGCACAAG GCACCAGAGC GTGCAGACTG AGGATGGCCC AGGTGGGGTG 
GCCAGGGCCC TGGCCAAGGC AGCGCTAAGT CCAGTGCAGG AACATGAGAC AGGCCGGCGC 
AGTAGCTCTG GTGAGGCAGG CACACCCCCG GTGCCCATTG TTGTAGAGCC TGCTCGGCCA 
GGGGTCAAGA CCCAGGCACC CCAGCCTCTG GGCACAGACT CCAAGGGATT GAAGGAACCT 
GTAGCCCAGA TGCCTCTGAT GCCTGATGCC CCAAGGGGCA GGGAGCGCTG GGTGCTAGAG 
GAGGTGGAAG AGCGGACCAC GCTAAGCGGA CTTCGCTCTA AGCCCGCCTC CCCCAAACTC 
TCTCCTGACC CCCAGACTCC AACCCTAGTC CCAACAAAGA ATGTACCCCG CAGTGCAGCT 
CCTTCAGTCC CACCTGCTTC CCTCATGGTC CCAGGCACCA AGCCTGAAGC AGGGTTGAAC 
TCCCGGTGTC CTGCTGAAGC TGTGACTCCT GCAGGCCTCA CCAAAACTGG GGCACCTAGC 
CCTGCATCTC TAGGTCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast1 ( XM_006531217.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531280.1 Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu41631
Clone ID Related Accession (Same CDS sequence)	XM_006531217.3 , XM_006531217.4
Accession Version	XM_006531217.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006531217.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGATT CTGGCCTGAT CCGCCGCCGG AGGCTGCAGG ATTTACCCCT GCCCCGGAAA 
AGCAGCAGTT GCCGTACCAG CAACCGGAAA AGCCTCATCC TGACAAGCAC TTCTCCGACT 
CTACCGCGAC CCCACTCCCC GCTGCCGGGC CACTTAGGTA GCAGTCCTTT GGACAGCCCC 
CGCAACTTCT CTCCCAACAC TCCCGCCCAC TTCTCGTTTG CTTCTTCCCG AAGGGCGGAT 
GGACGCCGAT GGTCGCTTGC TTCGCTCCCT TCCTCTGGCT ATGGCACCAA CACGCCCAGC 
TCAACTGTTT CATCCTCCTG CTCCTCCCAG GAGCGCCTGC ATCAACTACC GTACCAGCCC 
ACCGTGGACG AGCTGCACTT TCTGTCCAAA CACTTCGGCA GCACCGAGAG CATCACCGAC 
GAGGATGGTG GCCGCCGCTC TCCCGCCGTG CGTCCTCGCT CTCGCAGCCT CAGCCCCGGC 
CGCTCCCCCT CCTCCTACGA CAACGAAATT GTGATGATGA ACCATGTGTA CAAGGAGCGG 
TTCCCAAAGG CCACTGCACA GATGGAAGAG AAGCTGCGGG ACTTCGCCCG CGCCTATGAA 
CCTGACAGCG TGCTGCCGCT GGCCGACGGC GTGCTCAGCT TCATCCACCA TCAGATCATT 
GAACTGGCCC GAGACTGCCT TACCAAGTCC CGCGATGGGC TCATCACCAC GGTCTATTTC 
TATGAACTGC AGGAAAACCT AGAGAAGCTG CTGCAGGACG CTTATGAACG CTCGGAGAGC 
TTGGAGGTAG CCTTTGTCAC TCAGCTGGTG AAGAAACTTC TCATAATCAT CTCTCGCCCT 
GCAAGGCTTC TGGAGTGCCT GGAATTCAAC CCAGAAGAGT TCTACCACCT TCTGGAGGCA 
GCCGAGGGAC ATGCCAAGGA GGGCCACCTG GTCAAAACTG ACATCCCCCG ATACATCATC 
CGACAGCTGG GCCTCACTCG GGACCCCTTT CCAGATGTGG TGCGCCTGGA GGAGCAGGAC 
AGCGGGGGCT CCAACACTCC GGAGCAAGAC GACACATCTG AGGGTCGCAG CAGCACCTCC 
AAGGCCAAGA AACCTCCCGG TGAAAGTGAC TTTGACACTA TCAAGCTCAT TAGCAATGGT 
GCCTACGGAG CCGTCTACCT GGTGCGGCAC CGGGACACGA GGCAGCGCTT CGCCATGAAG 
AAGATCAACA AACAGAACCT GATCCTGCGC AACCAGATCC AGCAGGCGTT TGTGGAGCGC 
GACATCCTCA CGTTTGCAGA GAACCCATTT GTGGTCGGCA TGTTCTGCTC CTTTGAGACC 
CGGCGCCACC TTTGCATGGT CATGGAGTAT GTGGAAGGTG GTGACTGTGC CACTCTCCTC 
AAGAACATTG GGGCACTGCC TGTGGAGATG GCGCGAATGT ATTTTGCAGA AACAGTTCTA 
GCCCTTGAGT ACTTACACAA TTATGGCATC GTGCACCGTG ACCTCAAGCC AGACAACCTC 
CTCATTACTT CCATGGGCCA CATCAAGCTC ACGGATTTTG GCCTCTCCAA GATGGGACTC 
ATGAGCCTGA CCACCAACTT ATATGAGGGG CACATTGAGA AGGATGCCCG GGAGTTCCTG 
GACAAACAGG TGTGTGGGAC CCCAGAGTAC ATCGCACCCG AGGTCATCCT GCGCCAGGGC 
TACGGCAAGC CAGTGGACTG GTGGGCTATG GGGATCATTC TCTACGAGTT CCTGGTGGGC 
TGTGTGCCTT TCTTCGGGGA TACCCCAGAA GAGCTCTTTG GACAGGTCAT CAGCGATGAC 
ATACTGTGGC CTGAAGGGGA CGAGGCCCTG CCCACAGATG CACAGCTTCT AATATCTAGC 
CTCTTGCAGA CCAACCCCCT GGTCCGGCTT GGGGCAGGTG GTGCTTTTGA GGTGAAGCAG 
CATAGTTTCT TCCGAGACCT GGACTGGACA GGGCTACTGC GGCAGAAGGC GGAGTTCATC 
CCCCACCTAG AGTCAGAAGA TGACACCAGC TACTTTGACA CCCGCTCCGA CAGGTATCAC 
CATGTGAACT CCTATGATGA GGACGACACC ACCGAAGAGG AGCCCGTGGA GATCCGCCAA 
TTCTCCTCCT GCTCGCCGCG CTTCAGCAAG GTGTACAGCA GTATGGAGCA GCTGTCTCAG 
CACGAGCCTA AGACCCCCGT CTCAGCATCT GGGGCCAGCA AGCGGGACCC AAGCGCTAAG 
GGCCCGGAGG AGAAAGTGGC CGGCAAGAGG GAGGGACTGG GTGGCCTGAC TCTGCGTGAG 
AAGACCTGGA GAGGGGGCTC TCCAGAGATT AAGCGCTTCT CAGCGTCTGA AGCCAGTTTT 
CTGGAAGGAG AGGCCAGTCC CCCGCTGGGA GCGCGCAGAC GTTTCTCAGC ACTGCTGGAG 
CCCAGCCGCT TCACTGCCCC TCAAGAGGAT GAAGATGAGG CTCGACTCCG CAGACCTCCT 
CGACCCAGCT CCGATCCCCC TAGCTCACTG GACACACGCG TCCCCAAAGA GGCAGTTCAA 
GGAGAGGGCA CGTCCACCCC CGGGGAACCC GAGGCCACTG AGCGCTCACA TCCAGGTGAC 
CTCGGCCCAC CCTCCAAGGA TGGAGACCCA TCAGGCCCTA GGGCCACCAA CGACCTGGTT 
TTGCGAAGGG CAAGGCACCA GCAGCTGTCT GGGGATCTTG CTGTGGAGAA GCGACCGTCT 
CGAACTGGGG GCAAAGTTAT CAAGTCGGCT TCAGCTACAG CCTTGTCAGT CATGATTCCT 
GCAGTGGACC CACATGGAGG CTCACCCCTG GCTAGTCCCA TGTCCCCTCG ATCTCTCTCC 
TCCAATCCAT CCTCACGGGA CTCTTCGCCT AGCCGAGACT ACTCACCAGC TGTCAGTGGA 
CTCCGTTCCC CCATCACCAT CCAACGCTCA GGCAAGAAAT ATGGGTTCAC ACTGCGAGCC 
ATCCGTGTCT ACATGGGCGA CTCCGATGTC TACAGTGTCC ACCACATTGT TTGGCACGTG 
GAGGAAGGAG GCCCAGCCCA AGAGGCAGGG CTCTGTGCTG GGGACCTCAT CACTCACGTG 
AATGGTGAGC CCGTGCATGG TATGGTGCAT CCTGAGGTGG TAGAGCTGAT CCTGAAGAGT 
GGCAACAAAG TAGCTGTGAC CACCACACCC TTTGAAAACA CTTCCATCCG GATTGGGCCT 
GCCAGGCGCA GCAGTTACAA AGCCAAAATG GCCCGAAGGA ACAAAAGGCC TTCGGCCAAA 
GACGGCCAGG AGAGCAAGAA GCGGAGCTCT CTCTTTCGGA AGATCACAAA GCAGTCGAAC 
CTGCTGCACA CCAGTCGATC GCTGTCCTCT TTGAACCGGT CCTTGTCGTC CAGCGACAGT 
CTTCCGGGCT CGCCCACGCA CGGACTACCC GCACGCTCGC CCACACATAG CTATCGCTCT 
ACACCTGACT CCGCCTACCT AGGCGCCTCG TCACAGAGTA GCTCGCCAGC TTCCAGCACG 
CCTAATTCCC CCGCCTCATC CGCCTCGCAC CACATCAGGC CCAGCACCTT GCACGGGCTG 
TCACCGAAAC TACACCGCCA GTACCGCTCA GCGCGCTGTA AATCGGCTGG CAACATCCCG 
CTGTCACCTC TAGCGCACAC ACCATCCCCG ACGCAGGCGT CGCCGCCGCC GCTGCCAGGC 
CACACGGTGG GCAGCTCACA TACCACGCAG AGCTTCCCTG CCAAACTACA CTCATCGCCA 
CCTGTCGTGC GCCCGCGCCC CAAAAGTGCA GAGCCACCAC GCTCGCCGTT GCTCAAGCGC 
GTGCAGTCAG CGGAGAAACT AGGGGCTTCG CTGGGAGCAG ACAAGAAGGG CGCACTGCGC 
AAACACAGCC TGGAAGTGGG ACACCCTGAT TTCCGAAAGG ACTTCCATGG TGAGCTGGCC 
CTGCATAGCC TCGCTGAGTC TGATGGTGAG ACGCCTCCTA TTGAGGGTCC TGGGGCGACC 
CGGCAGGTCG CCGTGCGACG CCTGGGTCGC CAGGAATCAC CCCTAAGCTT GGGCGCGGAC 
CCGTTGCTAC CCGATGGTGT CCAGAGACCT ATGGCGTCAA GCAAGGAGGA TTCCGCGGGA 
GGTACAGAGG CTTGCACCCC ACCTCGCGCG ACAACCCCTG GGAGCCGGAC CCTGGAGCGT 
GACTCGGGTT GCACAAGGCA CCAGAGCGTG CAGACTGAGG ATGGCCCAGG TGGGGTGGCC 
AGGGCCCTGG CCAAGGCAGC GCTAAGTCCA GTGCAGGAAC ATGAGACAGG CCGGCGCAGT 
AGCTCTGGTG AGGCAGGCAC ACCCCCGGTG CCCATTGTTG TAGAGCCTGC TCGGCCAGGG 
GTCAAGACCC AGGCACCCCA GCCTCTGGGC ACAGACTCCA AGGGATTGAA GGAACCTGTA 
GCCCAGATGC CTCTGATGCC TGATGCCCCA AGGGGCAGGG AGCGCTGGGT GCTAGAGGAG 
GTGGAAGAGC GGACCACGCT AAGCGGACTT CGCTCTAAGC CCGCCTCCCC CAAACTCTCT 
CCTGACCCCC AGACTCCAAC CCTAGTCCCA ACAAAGAATG TACCCCGCAG TGCAGCTCCT 
TCAGTCCCAC CTGCTTCCCT CATGGTCCCA GGCACCAAGC CTGAAGCAGG GTTGAACTCC 
CGGTGTCCTG CTGAAGCTGT GACTCCTGCA GGCCTCACCA AAACTGGGGC ACCTAGCCCT 
GCATCTCTAG GTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531280.1
CDS	133..4830
Translation	MDDSGLIRRRRLQDLPLPRKSSSCRTSNRKSLILTSTSPTLPRPHSPLPGHLGSSPLDSPRNFSPNTPAHFSFASSRRADGRRWSLASLPSSGYGTNTPSSTVSSSCSSQERLHQLPYQPTVDELHFLSKHFGSTESITDEDGGRRSPAVRPRSRSLSPGRSPSSYDNEIVMMNHVYKERFPKATAQMEEKLRDFARAYEPDSVLPLADGVLSFIHHQIIELARDCLTKSRDGLITTVYFYELQENLEKLLQDAYERSESLEVAFVTQLVKKLLIIISRPARLLECLEFNPEEFYHLLEAAEGHAKEGHLVKTDIPRYIIRQLGLTRDPFPDVVRLEEQDSGGSNTPEQDDTSEGRSSTSKAKKPPGESDFDTIKLISNGAYGAVYLVRHRDTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNIGALPVEMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDDILWPEGDEALPTDAQLLISSLLQTNPLVRLGAGGAFEVKQHSFFRDLDWTGLLRQKAEFIPHLESEDDTSYFDTRSDRYHHVNSYDEDDTTEEEPVEIRQFSSCSPRFSKVYSSMEQLSQHEPKTPVSASGASKRDPSAKGPEEKVAGKREGLGGLTLREKTWRGGSPEIKRFSASEASFLEGEASPPLGARRRFSALLEPSRFTAPQEDEDEARLRRPPRPSSDPPSSLDTRVPKEAVQGEGTSTPGEPEATERSHPGDLGPPSKDGDPSGPRATNDLVLRRARHQQLSGDLAVEKRPSRTGGKVIKSASATALSVMIPAVDPHGGSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYGFTLRAIRVYMGDSDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVAVTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKDGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSLSSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASSQSSSPASSTPNSPASSASHHIRPSTLHGLSPKLHRQYRSARCKSAGNIPLSPLAHTPSPTQASPPPLPGHTVGSSHTTQSFPAKLHSSPPVVRPRPKSAEPPRSPLLKRVQSAEKLGASLGADKKGALRKHSLEVGHPDFRKDFHGELALHSLAESDGETPPIEGPGATRQVAVRRLGRQESPLSLGADPLLPDGVQRPMASSKEDSAGGTEACTPPRATTPGSRTLERDSGCTRHQSVQTEDGPGGVARALAKAALSPVQEHETGRRSSSGEAGTPPVPIVVEPARPGVKTQAPQPLGTDSKGLKEPVAQMPLMPDAPRGRERWVLEEVEERTTLSGLRSKPASPKLSPDPQTPTLVPTKNVPRSAAPSVPPASLMVPGTKPEAGLNSRCPAEAVTPAGLTKTGAPSPASLGP

Target ORF information:

RefSeq Version	XM_006531217.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531217.4

ORF Insert Sequence:

ATGGACGATT CTGGCCTGAT CCGCCGCCGG AGGCTGCAGG ATTTACCCCT GCCCCGGAAA 
AGCAGCAGTT GCCGTACCAG CAACCGGAAA AGCCTCATCC TGACAAGCAC TTCTCCGACT 
CTACCGCGAC CCCACTCCCC GCTGCCGGGC CACTTAGGTA GCAGTCCTTT GGACAGCCCC 
CGCAACTTCT CTCCCAACAC TCCCGCCCAC TTCTCGTTTG CTTCTTCCCG AAGGGCGGAT 
GGACGCCGAT GGTCGCTTGC TTCGCTCCCT TCCTCTGGCT ATGGCACCAA CACGCCCAGC 
TCAACTGTTT CATCCTCCTG CTCCTCCCAG GAGCGCCTGC ATCAACTACC GTACCAGCCC 
ACCGTGGACG AGCTGCACTT TCTGTCCAAA CACTTCGGCA GCACCGAGAG CATCACCGAC 
GAGGATGGTG GCCGCCGCTC TCCCGCCGTG CGTCCTCGCT CTCGCAGCCT CAGCCCCGGC 
CGCTCCCCCT CCTCCTACGA CAACGAAATT GTGATGATGA ACCATGTGTA CAAGGAGCGG 
TTCCCAAAGG CCACTGCACA GATGGAAGAG AAGCTGCGGG ACTTCGCCCG CGCCTATGAA 
CCTGACAGCG TGCTGCCGCT GGCCGACGGC GTGCTCAGCT TCATCCACCA TCAGATCATT 
GAACTGGCCC GAGACTGCCT TACCAAGTCC CGCGATGGGC TCATCACCAC GGTCTATTTC 
TATGAACTGC AGGAAAACCT AGAGAAGCTG CTGCAGGACG CTTATGAACG CTCGGAGAGC 
TTGGAGGTAG CCTTTGTCAC TCAGCTGGTG AAGAAACTTC TCATAATCAT CTCTCGCCCT 
GCAAGGCTTC TGGAGTGCCT GGAATTCAAC CCAGAAGAGT TCTACCACCT TCTGGAGGCA 
GCCGAGGGAC ATGCCAAGGA GGGCCACCTG GTCAAAACTG ACATCCCCCG ATACATCATC 
CGACAGCTGG GCCTCACTCG GGACCCCTTT CCAGATGTGG TGCGCCTGGA GGAGCAGGAC 
AGCGGGGGCT CCAACACTCC GGAGCAAGAC GACACATCTG AGGGTCGCAG CAGCACCTCC 
AAGGCCAAGA AACCTCCCGG TGAAAGTGAC TTTGACACTA TCAAGCTCAT TAGCAATGGT 
GCCTACGGAG CCGTCTACCT GGTGCGGCAC CGGGACACGA GGCAGCGCTT CGCCATGAAG 
AAGATCAACA AACAGAACCT GATCCTGCGC AACCAGATCC AGCAGGCGTT TGTGGAGCGC 
GACATCCTCA CGTTTGCAGA GAACCCATTT GTGGTCGGCA TGTTCTGCTC CTTTGAGACC 
CGGCGCCACC TTTGCATGGT CATGGAGTAT GTGGAAGGTG GTGACTGTGC CACTCTCCTC 
AAGAACATTG GGGCACTGCC TGTGGAGATG GCGCGAATGT ATTTTGCAGA AACAGTTCTA 
GCCCTTGAGT ACTTACACAA TTATGGCATC GTGCACCGTG ACCTCAAGCC AGACAACCTC 
CTCATTACTT CCATGGGCCA CATCAAGCTC ACGGATTTTG GCCTCTCCAA GATGGGACTC 
ATGAGCCTGA CCACCAACTT ATATGAGGGG CACATTGAGA AGGATGCCCG GGAGTTCCTG 
GACAAACAGG TGTGTGGGAC CCCAGAGTAC ATCGCACCCG AGGTCATCCT GCGCCAGGGC 
TACGGCAAGC CAGTGGACTG GTGGGCTATG GGGATCATTC TCTACGAGTT CCTGGTGGGC 
TGTGTGCCTT TCTTCGGGGA TACCCCAGAA GAGCTCTTTG GACAGGTCAT CAGCGATGAC 
ATACTGTGGC CTGAAGGGGA CGAGGCCCTG CCCACAGATG CACAGCTTCT AATATCTAGC 
CTCTTGCAGA CCAACCCCCT GGTCCGGCTT GGGGCAGGTG GTGCTTTTGA GGTGAAGCAG 
CATAGTTTCT TCCGAGACCT GGACTGGACA GGGCTACTGC GGCAGAAGGC GGAGTTCATC 
CCCCACCTAG AGTCAGAAGA TGACACCAGC TACTTTGACA CCCGCTCCGA CAGGTATCAC 
CATGTGAACT CCTATGATGA GGACGACACC ACCGAAGAGG AGCCCGTGGA GATCCGCCAA 
TTCTCCTCCT GCTCGCCGCG CTTCAGCAAG GTGTACAGCA GTATGGAGCA GCTGTCTCAG 
CACGAGCCTA AGACCCCCGT CTCAGCATCT GGGGCCAGCA AGCGGGACCC AAGCGCTAAG 
GGCCCGGAGG AGAAAGTGGC CGGCAAGAGG GAGGGACTGG GTGGCCTGAC TCTGCGTGAG 
AAGACCTGGA GAGGGGGCTC TCCAGAGATT AAGCGCTTCT CAGCGTCTGA AGCCAGTTTT 
CTGGAAGGAG AGGCCAGTCC CCCGCTGGGA GCGCGCAGAC GTTTCTCAGC ACTGCTGGAG 
CCCAGCCGCT TCACTGCCCC TCAAGAGGAT GAAGATGAGG CTCGACTCCG CAGACCTCCT 
CGACCCAGCT CCGATCCCCC TAGCTCACTG GACACACGCG TCCCCAAAGA GGCAGTTCAA 
GGAGAGGGCA CGTCCACCCC CGGGGAACCC GAGGCCACTG AGCGCTCACA TCCAGGTGAC 
CTCGGCCCAC CCTCCAAGGA TGGAGACCCA TCAGGCCCTA GGGCCACCAA CGACCTGGTT 
TTGCGAAGGG CAAGGCACCA GCAGCTGTCT GGGGATCTTG CTGTGGAGAA GCGACCGTCT 
CGAACTGGGG GCAAAGTTAT CAAGTCGGCT TCAGCTACAG CCTTGTCAGT CATGATTCCT 
GCAGTGGACC CACATGGAGG CTCACCCCTG GCTAGTCCCA TGTCCCCTCG ATCTCTCTCC 
TCCAATCCAT CCTCACGGGA CTCTTCGCCT AGCCGAGACT ACTCACCAGC TGTCAGTGGA 
CTCCGTTCCC CCATCACCAT CCAACGCTCA GGCAAGAAAT ATGGGTTCAC ACTGCGAGCC 
ATCCGTGTCT ACATGGGCGA CTCCGATGTC TACAGTGTCC ACCACATTGT TTGGCACGTG 
GAGGAAGGAG GCCCAGCCCA AGAGGCAGGG CTCTGTGCTG GGGACCTCAT CACTCACGTG 
AATGGTGAGC CCGTGCATGG TATGGTGCAT CCTGAGGTGG TAGAGCTGAT CCTGAAGAGT 
GGCAACAAAG TAGCTGTGAC CACCACACCC TTTGAAAACA CTTCCATCCG GATTGGGCCT 
GCCAGGCGCA GCAGTTACAA AGCCAAAATG GCCCGAAGGA ACAAAAGGCC TTCGGCCAAA 
GACGGCCAGG AGAGCAAGAA GCGGAGCTCT CTCTTTCGGA AGATCACAAA GCAGTCGAAC 
CTGCTGCACA CCAGTCGATC GCTGTCCTCT TTGAACCGGT CCTTGTCGTC CAGCGACAGT 
CTTCCGGGCT CGCCCACGCA CGGACTACCC GCACGCTCGC CCACACATAG CTATCGCTCT 
ACACCTGACT CCGCCTACCT AGGCGCCTCG TCACAGAGTA GCTCGCCAGC TTCCAGCACG 
CCTAATTCCC CCGCCTCATC CGCCTCGCAC CACATCAGGC CCAGCACCTT GCACGGGCTG 
TCACCGAAAC TACACCGCCA GTACCGCTCA GCGCGCTGTA AATCGGCTGG CAACATCCCG 
CTGTCACCTC TAGCGCACAC ACCATCCCCG ACGCAGGCGT CGCCGCCGCC GCTGCCAGGC 
CACACGGTGG GCAGCTCACA TACCACGCAG AGCTTCCCTG CCAAACTACA CTCATCGCCA 
CCTGTCGTGC GCCCGCGCCC CAAAAGTGCA GAGCCACCAC GCTCGCCGTT GCTCAAGCGC 
GTGCAGTCAG CGGAGAAACT AGGGGCTTCG CTGGGAGCAG ACAAGAAGGG CGCACTGCGC 
AAACACAGCC TGGAAGTGGG ACACCCTGAT TTCCGAAAGG ACTTCCATGG TGAGCTGGCC 
CTGCATAGCC TCGCTGAGTC TGATGGTGAG ACGCCTCCTA TTGAGGGTCC TGGGGCGACC 
CGGCAGGTCG CCGTGCGACG CCTGGGTCGC CAGGAATCAC CCCTAAGCTT GGGCGCGGAC 
CCGTTGCTAC CCGATGGTGT CCAGAGACCT ATGGCGTCAA GCAAGGAGGA TTCCGCGGGA 
GGTACAGAGG CTTGCACCCC ACCTCGCGCG ACAACCCCTG GGAGCCGGAC CCTGGAGCGT 
GACTCGGGTT GCACAAGGCA CCAGAGCGTG CAGACTGAGG ATGGCCCAGG TGGGGTGGCC 
AGGGCCCTGG CCAAGGCAGC GCTAAGTCCA GTGCAGGAAC ATGAGACAGG CCGGCGCAGT 
AGCTCTGGTG AGGCAGGCAC ACCCCCGGTG CCCATTGTTG TAGAGCCTGC TCGGCCAGGG 
GTCAAGACCC AGGCACCCCA GCCTCTGGGC ACAGACTCCA AGGGATTGAA GGAACCTGTA 
GCCCAGATGC CTCTGATGCC TGATGCCCCA AGGGGCAGGG AGCGCTGGGT GCTAGAGGAG 
GTGGAAGAGC GGACCACGCT AAGCGGACTT CGCTCTAAGC CCGCCTCCCC CAAACTCTCT 
CCTGACCCCC AGACTCCAAC CCTAGTCCCA ACAAAGAATG TACCCCGCAG TGCAGCTCCT 
TCAGTCCCAC CTGCTTCCCT CATGGTCCCA GGCACCAAGC CTGAAGCAGG GTTGAACTCC 
CGGTGTCCTG CTGAAGCTGT GACTCCTGCA GGCCTCACCA AAACTGGGGC ACCTAGCCCT 
GCATCTCTAG GTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast1 ( NM_019945.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064329.2 Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), mRNA.

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CloneID	OMu11725
Clone ID Related Accession (Same CDS sequence)	NM_019945.2
Accession Version	NM_019945.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC054524.1, AY312167.1 and BU701555.1. On Feb 10, 2007 this sequence version replaced NM_019945.1. ##Evidence-Data-START## Transcript exon combination :: BC054524.1, AF077818.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTCTGACT CTCTCTGGAC CGCGCTTTCC AATTTCTCGA TGCCCTCCTT CCCCGGCGGC 
AGCATGTTTC GCCGCACCAA GAGTTGCCGT ACCAGCAACC GGAAAAGCCT CATCCTGACA 
AGCACTTCTC CGACTCTACC GCGACCCCAC TCCCCGCTGC CGGGCCACTT AGGTAGCAGT 
CCTTTGGACA GCCCCCGCAA CTTCTCTCCC AACACTCCCG CCCACTTCTC GTTTGCTTCT 
TCCCGAAGGG CGGATGGACG CCGATGGTCG CTTGCTTCGC TCCCTTCCTC TGGCTATGGC 
ACCAACACGC CCAGCTCAAC TGTTTCATCC TCCTGCTCCT CCCAGGAGCG CCTGCATCAA 
CTACCGTACC AGCCCACCGT GGACGAGCTG CACTTTCTGT CCAAACACTT CGGCAGCACC 
GAGAGCATCA CCGACGAGGA TGGTGGCCGC CGCTCTCCCG CCGTGCGTCC TCGCTCTCGC 
AGCCTCAGCC CCGGCCGCTC CCCCTCCTCC TACGACAACG AAATTGTGAT GATGAACCAT 
GTGTACAAGG AGCGGTTCCC AAAGGCCACT GCACAGATGG AAGAGAAGCT GCGGGACTTC 
GCCCGCGCCT ATGAACCTGA CAGCGTGCTG CCGCTGGCCG ACGGCGTGCT CAGCTTCATC 
CACCATCAGA TCATTGAACT GGCCCGAGAC TGCCTTACCA AGTCCCGCGA TGGGCTCATC 
ACCACGGTCT ATTTCTATGA ACTGCAGGAA AACCTAGAGA AGCTGCTGCA GGACGCTTAT 
GAACGCTCGG AGAGCTTGGA GGTAGCCTTT GTCACTCAGC TGGTGAAGAA ACTTCTCATA 
ATCATCTCTC GCCCTGCAAG GCTTCTGGAG TGCCTGGAAT TCAACCCAGA AGAGTTCTAC 
CACCTTCTGG AGGCAGCCGA GGGACATGCC AAGGAGGGCC ACCTGGTCAA AACTGACATC 
CCCCGATACA TCATCCGACA GCTGGGCCTC ACTCGGGACC CCTTTCCAGA TGTGGTGCGC 
CTGGAGGAGC AGGACAGCGG GGGCTCCAAC ACTCCGGAGC AAGACGACAC ATCTGAGGGT 
CGCAGCAGCA CCTCCAAGGC CAAGAAACCT CCCGGTGAAA GTGACTTTGA CACTATCAAG 
CTCATTAGCA ATGGTGCCTA CGGAGCCGTC TACCTGGTGC GGCACCGGGA CACGAGGCAG 
CGCTTCGCCA TGAAGAAGAT CAACAAACAG AACCTGATCC TGCGCAACCA GATCCAGCAG 
GCGTTTGTGG AGCGCGACAT CCTCACGTTT GCAGAGAACC CATTTGTGGT CGGCATGTTC 
TGCTCCTTTG AGACCCGGCG CCACCTTTGC ATGGTCATGG AGTATGTGGA AGGTGGTGAC 
TGTGCCACTC TCCTCAAGAA CATTGGGGCA CTGCCTGTGG AGATGGCGCG AATGTATTTT 
GCAGAAACAG TTCTAGCCCT TGAGTACTTA CACAATTATG GCATCGTGCA CCGTGACCTC 
AAGCCAGACA ACCTCCTCAT TACTTCCATG GGCCACATCA AGCTCACGGA TTTTGGCCTC 
TCCAAGATGG GACTCATGAG CCTGACCACC AACTTATATG AGGGGCACAT TGAGAAGGAT 
GCCCGGGAGT TCCTGGACAA ACAGGTGTGT GGGACCCCAG AGTACATCGC ACCCGAGGTC 
ATCCTGCGCC AGGGCTACGG CAAGCCAGTG GACTGGTGGG CTATGGGGAT CATTCTCTAC 
GAGTTCCTGG TGGGCTGTGT GCCTTTCTTC GGGGATACCC CAGAAGAGCT CTTTGGACAG 
GTCATCAGCG ATGACATACT GTGGCCTGAA GGGGACGAGG CCCTGCCCAC AGATGCACAG 
CTTCTAATAT CTAGCCTCTT GCAGACCAAC CCCCTGGTCC GGCTTGGGGC AGGTGGTGCT 
TTTGAGGTGA AGCAGCATAG TTTCTTCCGA GACCTGGACT GGACAGGGCT ACTGCGGCAG 
AAGGCGGAGT TCATCCCCCA CCTAGAGTCA GAAGATGACA CCAGCTACTT TGACACCCGC 
TCCGACAGGT ATCACCATGT GAACTCCTAT GATGAGGACG ACACCACCGA AGAGGAGCCC 
GTGGAGATCC GCCAATTCTC CTCCTGCTCG CCGCGCTTCA GCAAGGTGTA CAGCAGTATG 
GAGCAGCTGT CTCAGCACGA GCCTAAGACC CCCGTCTCAG CATCTGGGGC CAGCAAGCGG 
GACCCAAGCG CTAAGGGCCC GGAGGAGAAA GTGGCCGGCA AGAGGGAGGG ACTGGGTGGC 
CTGACTCTGC GTGAGAAGAC CTGGAGAGGG GGCTCTCCAG AGATTAAGCG CTTCTCAGCG 
TCTGAAGCCA GTTTTCTGGA AGGAGAGGCC AGTCCCCCGC TGGGAGCGCG CAGACGTTTC 
TCAGCACTGC TGGAGCCCAG CCGCTTCACT GCCCCTCAAG AGGATGAAGA TGAGGCTCGA 
CTCCGCAGAC CTCCTCGACC CAGCTCCGAT CCCCCTAGCT CACTGGACAC ACGCGTCCCC 
AAAGAGGCAG TTCAAGGAGA GGGCACGTCC ACCCCCGGGG AACCCGAGGC CACTGAGCGC 
TCACATCCAG GTGACCTCGG CCCACCCTCC AAGGATGGAG ACCCATCAGG CCCTAGGGCC 
ACCAACGACC TGGTTTTGCG AAGGGCAAGG CACCAGCAGC TGTCTGGGGA TCTTGCTGTG 
GAGAAGCGAC CGTCTCGAAC TGGGGGCAAA GTTATCAAGT CGGCTTCAGC TACAGCCTTG 
TCAGTCATGA TTCCTGCAGT GGACCCACAT GGAGGCTCAC CCCTGGCTAG TCCCATGTCC 
CCTCGATCTC TCTCCTCCAA TCCATCCTCA CGGGACTCTT CGCCTAGCCG AGACTACTCA 
CCAGCTGTCA GTGGACTCCG TTCCCCCATC ACCATCCAAC GCTCAGGCAA GAAATATGGG 
TTCACACTGC GAGCCATCCG TGTCTACATG GGCGACTCCG ATGTCTACAG TGTCCACCAC 
ATTGTTTGGC ACGTGGAGGA AGGAGGCCCA GCCCAAGAGG CAGGGCTCTG TGCTGGGGAC 
CTCATCACTC ACGTGAATGG TGAGCCCGTG CATGGTATGG TGCATCCTGA GGTGGTAGAG 
CTGATCCTGA AGAGTGGCAA CAAAGTAGCT GTGACCACCA CACCCTTTGA AAACACTTCC 
ATCCGGATTG GGCCTGCCAG GCGCAGCAGT TACAAAGCCA AAATGGCCCG AAGGAACAAA 
AGGCCTTCGG CCAAAGACGG CCAGGAGAGC AAGAAGCGGA GCTCTCTCTT TCGGAAGATC 
ACAAAGCAGT CGAACCTGCT GCACACCAGT CGATCGCTGT CCTCTTTGAA CCGGTCCTTG 
TCGTCCAGCG ACAGTCTTCC GGGCTCGCCC ACGCACGGAC TACCCGCACG CTCGCCCACA 
CATAGCTATC GCTCTACACC TGACTCCGCC TACCTAGGCG CCTCGTCACA GAGTAGCTCG 
CCAGCTTCCA GCACGCCTAA TTCCCCCGCC TCATCCGCCT CGCACCACAT CAGGCCCAGC 
ACCTTGCACG GGCTGTCACC GAAACTACAC CGCCAGTACC GCTCAGCGCG CTGTAAATCG 
GCTGGCAACA TCCCGCTGTC ACCTCTAGCG CACACACCAT CCCCGACGCA GGCGTCGCCG 
CCGCCGCTGC CAGGCCACAC GGTGGGCAGC TCACATACCA CGCAGAGCTT CCCTGCCAAA 
CTACACTCAT CGCCACCTGT CGTGCGCCCG CGCCCCAAAA GTGCAGAGCC ACCACGCTCG 
CCGTTGCTCA AGCGCGTGCA GTCAGCGGAG AAACTAGGGG CTTCGCTGGG AGCAGACAAG 
AAGGGCGCAC TGCGCAAACA CAGCCTGGAA GTGGGACACC CTGATTTCCG AAAGGACTTC 
CATGGTGAGC TGGCCCTGCA TAGCCTCGCT GAGTCTGATG GTGAGACGCC TCCTATTGAG 
GGTCCTGGGG CGACCCGGCA GGTCGCCGTG CGACGCCTGG GTCGCCAGGA ATCACCCCTA 
AGCTTGGGCG CGGACCCGTT GCTACCCGAT GGTGTCCAGA GACCTATGGC GTCAAGCAAG 
GAGGATTCCG CGGGAGGTAC AGAGGCTTGC ACCCCACCTC GCGCGACAAC CCCTGGGAGC 
CGGACCCTGG AGCGTGACTC GGGTTGCACA AGGCACCAGA GCGTGCAGAC TGAGGATGGC 
CCAGGTGGGG TGGCCAGGGC CCTGGCCAAG GCAGCGCTAA GTCCAGTGCA GGAACATGAG 
ACAGGCCGGC GCAGTAGCTC TGGTGAGGCA GGCACACCCC CGGTGCCCAT TGTTGTAGAG 
CCTGCTCGGC CAGGGGTCAA GACCCAGGCA CCCCAGCCTC TGGGCACAGA CTCCAAGGGA 
TTGAAGGAAC CTGTAGCCCA GATGCCTCTG ATGCCTGATG CCCCAAGGGG CAGGGAGCGC 
TGGGTGCTAG AGGAGGTGGA AGAGCGGACC ACGCTAAGCG GACTTCGCTC TAAGCCCGCC 
TCCCCCAAAC TCTCTCCTGA CCCCCAGACT CCAACCCTAG TCCCAACAAA GAATGTACCC 
CGCAGTGCAG CTCCTTCAGT CCCACCTGCT TCCCTCATGG TCCCAGGCAC CAAGCCTGAA 
GCAGGGTTGA ACTCCCGGTG TCCTGCTGAA GCTGTGACTC CTGCAGGCCT CACCAAAACT 
GGGGCACCTA GCCCTGCATC TCTAGGTCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064329.2
CDS	77..4789
Translation	MSDSLWTALSNFSMPSFPGGSMFRRTKSCRTSNRKSLILTSTSPTLPRPHSPLPGHLGSSPLDSPRNFSPNTPAHFSFASSRRADGRRWSLASLPSSGYGTNTPSSTVSSSCSSQERLHQLPYQPTVDELHFLSKHFGSTESITDEDGGRRSPAVRPRSRSLSPGRSPSSYDNEIVMMNHVYKERFPKATAQMEEKLRDFARAYEPDSVLPLADGVLSFIHHQIIELARDCLTKSRDGLITTVYFYELQENLEKLLQDAYERSESLEVAFVTQLVKKLLIIISRPARLLECLEFNPEEFYHLLEAAEGHAKEGHLVKTDIPRYIIRQLGLTRDPFPDVVRLEEQDSGGSNTPEQDDTSEGRSSTSKAKKPPGESDFDTIKLISNGAYGAVYLVRHRDTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNIGALPVEMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDDILWPEGDEALPTDAQLLISSLLQTNPLVRLGAGGAFEVKQHSFFRDLDWTGLLRQKAEFIPHLESEDDTSYFDTRSDRYHHVNSYDEDDTTEEEPVEIRQFSSCSPRFSKVYSSMEQLSQHEPKTPVSASGASKRDPSAKGPEEKVAGKREGLGGLTLREKTWRGGSPEIKRFSASEASFLEGEASPPLGARRRFSALLEPSRFTAPQEDEDEARLRRPPRPSSDPPSSLDTRVPKEAVQGEGTSTPGEPEATERSHPGDLGPPSKDGDPSGPRATNDLVLRRARHQQLSGDLAVEKRPSRTGGKVIKSASATALSVMIPAVDPHGGSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYGFTLRAIRVYMGDSDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVAVTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKDGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSLSSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASSQSSSPASSTPNSPASSASHHIRPSTLHGLSPKLHRQYRSARCKSAGNIPLSPLAHTPSPTQASPPPLPGHTVGSSHTTQSFPAKLHSSPPVVRPRPKSAEPPRSPLLKRVQSAEKLGASLGADKKGALRKHSLEVGHPDFRKDFHGELALHSLAESDGETPPIEGPGATRQVAVRRLGRQESPLSLGADPLLPDGVQRPMASSKEDSAGGTEACTPPRATTPGSRTLERDSGCTRHQSVQTEDGPGGVARALAKAALSPVQEHETGRRSSSGEAGTPPVPIVVEPARPGVKTQAPQPLGTDSKGLKEPVAQMPLMPDAPRGRERWVLEEVEERTTLSGLRSKPASPKLSPDPQTPTLVPTKNVPRSAAPSVPPASLMVPGTKPEAGLNSRCPAEAVTPAGLTKTGAPSPASLGP

Target ORF information:

RefSeq Version	NM_019945.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019945.2

ORF Insert Sequence:

ATGTCTGACT CTCTCTGGAC CGCGCTTTCC AATTTCTCGA TGCCCTCCTT CCCCGGCGGC 
AGCATGTTTC GCCGCACCAA GAGTTGCCGT ACCAGCAACC GGAAAAGCCT CATCCTGACA 
AGCACTTCTC CGACTCTACC GCGACCCCAC TCCCCGCTGC CGGGCCACTT AGGTAGCAGT 
CCTTTGGACA GCCCCCGCAA CTTCTCTCCC AACACTCCCG CCCACTTCTC GTTTGCTTCT 
TCCCGAAGGG CGGATGGACG CCGATGGTCG CTTGCTTCGC TCCCTTCCTC TGGCTATGGC 
ACCAACACGC CCAGCTCAAC TGTTTCATCC TCCTGCTCCT CCCAGGAGCG CCTGCATCAA 
CTACCGTACC AGCCCACCGT GGACGAGCTG CACTTTCTGT CCAAACACTT CGGCAGCACC 
GAGAGCATCA CCGACGAGGA TGGTGGCCGC CGCTCTCCCG CCGTGCGTCC TCGCTCTCGC 
AGCCTCAGCC CCGGCCGCTC CCCCTCCTCC TACGACAACG AAATTGTGAT GATGAACCAT 
GTGTACAAGG AGCGGTTCCC AAAGGCCACT GCACAGATGG AAGAGAAGCT GCGGGACTTC 
GCCCGCGCCT ATGAACCTGA CAGCGTGCTG CCGCTGGCCG ACGGCGTGCT CAGCTTCATC 
CACCATCAGA TCATTGAACT GGCCCGAGAC TGCCTTACCA AGTCCCGCGA TGGGCTCATC 
ACCACGGTCT ATTTCTATGA ACTGCAGGAA AACCTAGAGA AGCTGCTGCA GGACGCTTAT 
GAACGCTCGG AGAGCTTGGA GGTAGCCTTT GTCACTCAGC TGGTGAAGAA ACTTCTCATA 
ATCATCTCTC GCCCTGCAAG GCTTCTGGAG TGCCTGGAAT TCAACCCAGA AGAGTTCTAC 
CACCTTCTGG AGGCAGCCGA GGGACATGCC AAGGAGGGCC ACCTGGTCAA AACTGACATC 
CCCCGATACA TCATCCGACA GCTGGGCCTC ACTCGGGACC CCTTTCCAGA TGTGGTGCGC 
CTGGAGGAGC AGGACAGCGG GGGCTCCAAC ACTCCGGAGC AAGACGACAC ATCTGAGGGT 
CGCAGCAGCA CCTCCAAGGC CAAGAAACCT CCCGGTGAAA GTGACTTTGA CACTATCAAG 
CTCATTAGCA ATGGTGCCTA CGGAGCCGTC TACCTGGTGC GGCACCGGGA CACGAGGCAG 
CGCTTCGCCA TGAAGAAGAT CAACAAACAG AACCTGATCC TGCGCAACCA GATCCAGCAG 
GCGTTTGTGG AGCGCGACAT CCTCACGTTT GCAGAGAACC CATTTGTGGT CGGCATGTTC 
TGCTCCTTTG AGACCCGGCG CCACCTTTGC ATGGTCATGG AGTATGTGGA AGGTGGTGAC 
TGTGCCACTC TCCTCAAGAA CATTGGGGCA CTGCCTGTGG AGATGGCGCG AATGTATTTT 
GCAGAAACAG TTCTAGCCCT TGAGTACTTA CACAATTATG GCATCGTGCA CCGTGACCTC 
AAGCCAGACA ACCTCCTCAT TACTTCCATG GGCCACATCA AGCTCACGGA TTTTGGCCTC 
TCCAAGATGG GACTCATGAG CCTGACCACC AACTTATATG AGGGGCACAT TGAGAAGGAT 
GCCCGGGAGT TCCTGGACAA ACAGGTGTGT GGGACCCCAG AGTACATCGC ACCCGAGGTC 
ATCCTGCGCC AGGGCTACGG CAAGCCAGTG GACTGGTGGG CTATGGGGAT CATTCTCTAC 
GAGTTCCTGG TGGGCTGTGT GCCTTTCTTC GGGGATACCC CAGAAGAGCT CTTTGGACAG 
GTCATCAGCG ATGACATACT GTGGCCTGAA GGGGACGAGG CCCTGCCCAC AGATGCACAG 
CTTCTAATAT CTAGCCTCTT GCAGACCAAC CCCCTGGTCC GGCTTGGGGC AGGTGGTGCT 
TTTGAGGTGA AGCAGCATAG TTTCTTCCGA GACCTGGACT GGACAGGGCT ACTGCGGCAG 
AAGGCGGAGT TCATCCCCCA CCTAGAGTCA GAAGATGACA CCAGCTACTT TGACACCCGC 
TCCGACAGGT ATCACCATGT GAACTCCTAT GATGAGGACG ACACCACCGA AGAGGAGCCC 
GTGGAGATCC GCCAATTCTC CTCCTGCTCG CCGCGCTTCA GCAAGGTGTA CAGCAGTATG 
GAGCAGCTGT CTCAGCACGA GCCTAAGACC CCCGTCTCAG CATCTGGGGC CAGCAAGCGG 
GACCCAAGCG CTAAGGGCCC GGAGGAGAAA GTGGCCGGCA AGAGGGAGGG ACTGGGTGGC 
CTGACTCTGC GTGAGAAGAC CTGGAGAGGG GGCTCTCCAG AGATTAAGCG CTTCTCAGCG 
TCTGAAGCCA GTTTTCTGGA AGGAGAGGCC AGTCCCCCGC TGGGAGCGCG CAGACGTTTC 
TCAGCACTGC TGGAGCCCAG CCGCTTCACT GCCCCTCAAG AGGATGAAGA TGAGGCTCGA 
CTCCGCAGAC CTCCTCGACC CAGCTCCGAT CCCCCTAGCT CACTGGACAC ACGCGTCCCC 
AAAGAGGCAG TTCAAGGAGA GGGCACGTCC ACCCCCGGGG AACCCGAGGC CACTGAGCGC 
TCACATCCAG GTGACCTCGG CCCACCCTCC AAGGATGGAG ACCCATCAGG CCCTAGGGCC 
ACCAACGACC TGGTTTTGCG AAGGGCAAGG CACCAGCAGC TGTCTGGGGA TCTTGCTGTG 
GAGAAGCGAC CGTCTCGAAC TGGGGGCAAA GTTATCAAGT CGGCTTCAGC TACAGCCTTG 
TCAGTCATGA TTCCTGCAGT GGACCCACAT GGAGGCTCAC CCCTGGCTAG TCCCATGTCC 
CCTCGATCTC TCTCCTCCAA TCCATCCTCA CGGGACTCTT CGCCTAGCCG AGACTACTCA 
CCAGCTGTCA GTGGACTCCG TTCCCCCATC ACCATCCAAC GCTCAGGCAA GAAATATGGG 
TTCACACTGC GAGCCATCCG TGTCTACATG GGCGACTCCG ATGTCTACAG TGTCCACCAC 
ATTGTTTGGC ACGTGGAGGA AGGAGGCCCA GCCCAAGAGG CAGGGCTCTG TGCTGGGGAC 
CTCATCACTC ACGTGAATGG TGAGCCCGTG CATGGTATGG TGCATCCTGA GGTGGTAGAG 
CTGATCCTGA AGAGTGGCAA CAAAGTAGCT GTGACCACCA CACCCTTTGA AAACACTTCC 
ATCCGGATTG GGCCTGCCAG GCGCAGCAGT TACAAAGCCA AAATGGCCCG AAGGAACAAA 
AGGCCTTCGG CCAAAGACGG CCAGGAGAGC AAGAAGCGGA GCTCTCTCTT TCGGAAGATC 
ACAAAGCAGT CGAACCTGCT GCACACCAGT CGATCGCTGT CCTCTTTGAA CCGGTCCTTG 
TCGTCCAGCG ACAGTCTTCC GGGCTCGCCC ACGCACGGAC TACCCGCACG CTCGCCCACA 
CATAGCTATC GCTCTACACC TGACTCCGCC TACCTAGGCG CCTCGTCACA GAGTAGCTCG 
CCAGCTTCCA GCACGCCTAA TTCCCCCGCC TCATCCGCCT CGCACCACAT CAGGCCCAGC 
ACCTTGCACG GGCTGTCACC GAAACTACAC CGCCAGTACC GCTCAGCGCG CTGTAAATCG 
GCTGGCAACA TCCCGCTGTC ACCTCTAGCG CACACACCAT CCCCGACGCA GGCGTCGCCG 
CCGCCGCTGC CAGGCCACAC GGTGGGCAGC TCACATACCA CGCAGAGCTT CCCTGCCAAA 
CTACACTCAT CGCCACCTGT CGTGCGCCCG CGCCCCAAAA GTGCAGAGCC ACCACGCTCG 
CCGTTGCTCA AGCGCGTGCA GTCAGCGGAG AAACTAGGGG CTTCGCTGGG AGCAGACAAG 
AAGGGCGCAC TGCGCAAACA CAGCCTGGAA GTGGGACACC CTGATTTCCG AAAGGACTTC 
CATGGTGAGC TGGCCCTGCA TAGCCTCGCT GAGTCTGATG GTGAGACGCC TCCTATTGAG 
GGTCCTGGGG CGACCCGGCA GGTCGCCGTG CGACGCCTGG GTCGCCAGGA ATCACCCCTA 
AGCTTGGGCG CGGACCCGTT GCTACCCGAT GGTGTCCAGA GACCTATGGC GTCAAGCAAG 
GAGGATTCCG CGGGAGGTAC AGAGGCTTGC ACCCCACCTC GCGCGACAAC CCCTGGGAGC 
CGGACCCTGG AGCGTGACTC GGGTTGCACA AGGCACCAGA GCGTGCAGAC TGAGGATGGC 
CCAGGTGGGG TGGCCAGGGC CCTGGCCAAG GCAGCGCTAA GTCCAGTGCA GGAACATGAG 
ACAGGCCGGC GCAGTAGCTC TGGTGAGGCA GGCACACCCC CGGTGCCCAT TGTTGTAGAG 
CCTGCTCGGC CAGGGGTCAA GACCCAGGCA CCCCAGCCTC TGGGCACAGA CTCCAAGGGA 
TTGAAGGAAC CTGTAGCCCA GATGCCTCTG ATGCCTGATG CCCCAAGGGG CAGGGAGCGC 
TGGGTGCTAG AGGAGGTGGA AGAGCGGACC ACGCTAAGCG GACTTCGCTC TAAGCCCGCC 
TCCCCCAAAC TCTCTCCTGA CCCCCAGACT CCAACCCTAG TCCCAACAAA GAATGTACCC 
CGCAGTGCAG CTCCTTCAGT CCCACCTGCT TCCCTCATGG TCCCAGGCAC CAAGCCTGAA 
GCAGGGTTGA ACTCCCGGTG TCCTGCTGAA GCTGTGACTC CTGCAGGCCT CACCAAAACT 
GGGGCACCTA GCCCTGCATC TCTAGGTCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast1 ( XM_006531216.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531279.1 Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X1, mRNA.

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CloneID	OMu41630
Clone ID Related Accession (Same CDS sequence)	XM_006531216.4 , XM_006531216.3
Accession Version	XM_006531216.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531216.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGATT CTGGCCTGAT CCGCCGCCGG AGGCTGCAGA AGGATTTACC CCTGCCCCGG 
AAAAGCAGCA GTTGCCGTAC CAGCAACCGG AAAAGCCTCA TCCTGACAAG CACTTCTCCG 
ACTCTACCGC GACCCCACTC CCCGCTGCCG GGCCACTTAG GTAGCAGTCC TTTGGACAGC 
CCCCGCAACT TCTCTCCCAA CACTCCCGCC CACTTCTCGT TTGCTTCTTC CCGAAGGGCG 
GATGGACGCC GATGGTCGCT TGCTTCGCTC CCTTCCTCTG GCTATGGCAC CAACACGCCC 
AGCTCAACTG TTTCATCCTC CTGCTCCTCC CAGGAGCGCC TGCATCAACT ACCGTACCAG 
CCCACCGTGG ACGAGCTGCA CTTTCTGTCC AAACACTTCG GCAGCACCGA GAGCATCACC 
GACGAGGATG GTGGCCGCCG CTCTCCCGCC GTGCGTCCTC GCTCTCGCAG CCTCAGCCCC 
GGCCGCTCCC CCTCCTCCTA CGACAACGAA ATTGTGATGA TGAACCATGT GTACAAGGAG 
CGGTTCCCAA AGGCCACTGC ACAGATGGAA GAGAAGCTGC GGGACTTCGC CCGCGCCTAT 
GAACCTGACA GCGTGCTGCC GCTGGCCGAC GGCGTGCTCA GCTTCATCCA CCATCAGATC 
ATTGAACTGG CCCGAGACTG CCTTACCAAG TCCCGCGATG GGCTCATCAC CACGGTCTAT 
TTCTATGAAC TGCAGGAAAA CCTAGAGAAG CTGCTGCAGG ACGCTTATGA ACGCTCGGAG 
AGCTTGGAGG TAGCCTTTGT CACTCAGCTG GTGAAGAAAC TTCTCATAAT CATCTCTCGC 
CCTGCAAGGC TTCTGGAGTG CCTGGAATTC AACCCAGAAG AGTTCTACCA CCTTCTGGAG 
GCAGCCGAGG GACATGCCAA GGAGGGCCAC CTGGTCAAAA CTGACATCCC CCGATACATC 
ATCCGACAGC TGGGCCTCAC TCGGGACCCC TTTCCAGATG TGGTGCGCCT GGAGGAGCAG 
GACAGCGGGG GCTCCAACAC TCCGGAGCAA GACGACACAT CTGAGGGTCG CAGCAGCACC 
TCCAAGGCCA AGAAACCTCC CGGTGAAAGT GACTTTGACA CTATCAAGCT CATTAGCAAT 
GGTGCCTACG GAGCCGTCTA CCTGGTGCGG CACCGGGACA CGAGGCAGCG CTTCGCCATG 
AAGAAGATCA ACAAACAGAA CCTGATCCTG CGCAACCAGA TCCAGCAGGC GTTTGTGGAG 
CGCGACATCC TCACGTTTGC AGAGAACCCA TTTGTGGTCG GCATGTTCTG CTCCTTTGAG 
ACCCGGCGCC ACCTTTGCAT GGTCATGGAG TATGTGGAAG GTGGTGACTG TGCCACTCTC 
CTCAAGAACA TTGGGGCACT GCCTGTGGAG ATGGCGCGAA TGTATTTTGC AGAAACAGTT 
CTAGCCCTTG AGTACTTACA CAATTATGGC ATCGTGCACC GTGACCTCAA GCCAGACAAC 
CTCCTCATTA CTTCCATGGG CCACATCAAG CTCACGGATT TTGGCCTCTC CAAGATGGGA 
CTCATGAGCC TGACCACCAA CTTATATGAG GGGCACATTG AGAAGGATGC CCGGGAGTTC 
CTGGACAAAC AGGTGTGTGG GACCCCAGAG TACATCGCAC CCGAGGTCAT CCTGCGCCAG 
GGCTACGGCA AGCCAGTGGA CTGGTGGGCT ATGGGGATCA TTCTCTACGA GTTCCTGGTG 
GGCTGTGTGC CTTTCTTCGG GGATACCCCA GAAGAGCTCT TTGGACAGGT CATCAGCGAT 
GACATACTGT GGCCTGAAGG GGACGAGGCC CTGCCCACAG ATGCACAGCT TCTAATATCT 
AGCCTCTTGC AGACCAACCC CCTGGTCCGG CTTGGGGCAG GTGGTGCTTT TGAGGTGAAG 
CAGCATAGTT TCTTCCGAGA CCTGGACTGG ACAGGGCTAC TGCGGCAGAA GGCGGAGTTC 
ATCCCCCACC TAGAGTCAGA AGATGACACC AGCTACTTTG ACACCCGCTC CGACAGGTAT 
CACCATGTGA ACTCCTATGA TGAGGACGAC ACCACCGAAG AGGAGCCCGT GGAGATCCGC 
CAATTCTCCT CCTGCTCGCC GCGCTTCAGC AAGGTGTACA GCAGTATGGA GCAGCTGTCT 
CAGCACGAGC CTAAGACCCC CGTCTCAGCA TCTGGGGCCA GCAAGCGGGA CCCAAGCGCT 
AAGGGCCCGG AGGAGAAAGT GGCCGGCAAG AGGGAGGGAC TGGGTGGCCT GACTCTGCGT 
GAGAAGACCT GGAGAGGGGG CTCTCCAGAG ATTAAGCGCT TCTCAGCGTC TGAAGCCAGT 
TTTCTGGAAG GAGAGGCCAG TCCCCCGCTG GGAGCGCGCA GACGTTTCTC AGCACTGCTG 
GAGCCCAGCC GCTTCACTGC CCCTCAAGAG GATGAAGATG AGGCTCGACT CCGCAGACCT 
CCTCGACCCA GCTCCGATCC CCCTAGCTCA CTGGACACAC GCGTCCCCAA AGAGGCAGTT 
CAAGGAGAGG GCACGTCCAC CCCCGGGGAA CCCGAGGCCA CTGAGCGCTC ACATCCAGGT 
GACCTCGGCC CACCCTCCAA GGATGGAGAC CCATCAGGCC CTAGGGCCAC CAACGACCTG 
GTTTTGCGAA GGGCAAGGCA CCAGCAGCTG TCTGGGGATC TTGCTGTGGA GAAGCGACCG 
TCTCGAACTG GGGGCAAAGT TATCAAGTCG GCTTCAGCTA CAGCCTTGTC AGTCATGATT 
CCTGCAGTGG ACCCACATGG AGGCTCACCC CTGGCTAGTC CCATGTCCCC TCGATCTCTC 
TCCTCCAATC CATCCTCACG GGACTCTTCG CCTAGCCGAG ACTACTCACC AGCTGTCAGT 
GGACTCCGTT CCCCCATCAC CATCCAACGC TCAGGCAAGA AATATGGGTT CACACTGCGA 
GCCATCCGTG TCTACATGGG CGACTCCGAT GTCTACAGTG TCCACCACAT TGTTTGGCAC 
GTGGAGGAAG GAGGCCCAGC CCAAGAGGCA GGGCTCTGTG CTGGGGACCT CATCACTCAC 
GTGAATGGTG AGCCCGTGCA TGGTATGGTG CATCCTGAGG TGGTAGAGCT GATCCTGAAG 
AGTGGCAACA AAGTAGCTGT GACCACCACA CCCTTTGAAA ACACTTCCAT CCGGATTGGG 
CCTGCCAGGC GCAGCAGTTA CAAAGCCAAA ATGGCCCGAA GGAACAAAAG GCCTTCGGCC 
AAAGACGGCC AGGAGAGCAA GAAGCGGAGC TCTCTCTTTC GGAAGATCAC AAAGCAGTCG 
AACCTGCTGC ACACCAGTCG ATCGCTGTCC TCTTTGAACC GGTCCTTGTC GTCCAGCGAC 
AGTCTTCCGG GCTCGCCCAC GCACGGACTA CCCGCACGCT CGCCCACACA TAGCTATCGC 
TCTACACCTG ACTCCGCCTA CCTAGGCGCC TCGTCACAGA GTAGCTCGCC AGCTTCCAGC 
ACGCCTAATT CCCCCGCCTC ATCCGCCTCG CACCACATCA GGCCCAGCAC CTTGCACGGG 
CTGTCACCGA AACTACACCG CCAGTACCGC TCAGCGCGCT GTAAATCGGC TGGCAACATC 
CCGCTGTCAC CTCTAGCGCA CACACCATCC CCGACGCAGG CGTCGCCGCC GCCGCTGCCA 
GGCCACACGG TGGGCAGCTC ACATACCACG CAGAGCTTCC CTGCCAAACT ACACTCATCG 
CCACCTGTCG TGCGCCCGCG CCCCAAAAGT GCAGAGCCAC CACGCTCGCC GTTGCTCAAG 
CGCGTGCAGT CAGCGGAGAA ACTAGGGGCT TCGCTGGGAG CAGACAAGAA GGGCGCACTG 
CGCAAACACA GCCTGGAAGT GGGACACCCT GATTTCCGAA AGGACTTCCA TGGTGAGCTG 
GCCCTGCATA GCCTCGCTGA GTCTGATGGT GAGACGCCTC CTATTGAGGG TCCTGGGGCG 
ACCCGGCAGG TCGCCGTGCG ACGCCTGGGT CGCCAGGAAT CACCCCTAAG CTTGGGCGCG 
GACCCGTTGC TACCCGATGG TGTCCAGAGA CCTATGGCGT CAAGCAAGGA GGATTCCGCG 
GGAGGTACAG AGGCTTGCAC CCCACCTCGC GCGACAACCC CTGGGAGCCG GACCCTGGAG 
CGTGACTCGG GTTGCACAAG GCACCAGAGC GTGCAGACTG AGGATGGCCC AGGTGGGGTG 
GCCAGGGCCC TGGCCAAGGC AGCGCTAAGT CCAGTGCAGG AACATGAGAC AGGCCGGCGC 
AGTAGCTCTG GTGAGGCAGG CACACCCCCG GTGCCCATTG TTGTAGAGCC TGCTCGGCCA 
GGGGTCAAGA CCCAGGCACC CCAGCCTCTG GGCACAGACT CCAAGGGATT GAAGGAACCT 
GTAGCCCAGA TGCCTCTGAT GCCTGATGCC CCAAGGGGCA GGGAGCGCTG GGTGCTAGAG 
GAGGTGGAAG AGCGGACCAC GCTAAGCGGA CTTCGCTCTA AGCCCGCCTC CCCCAAACTC 
TCTCCTGACC CCCAGACTCC AACCCTAGTC CCAACAAAGA ATGTACCCCG CAGTGCAGCT 
CCTTCAGTCC CACCTGCTTC CCTCATGGTC CCAGGCACCA AGCCTGAAGC AGGGTTGAAC 
TCCCGGTGTC CTGCTGAAGC TGTGACTCCT GCAGGCCTCA CCAAAACTGG GGCACCTAGC 
CCTGCATCTC TAGGTCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531279.1
CDS	87..4787
Translation	MDDSGLIRRRRLQKDLPLPRKSSSCRTSNRKSLILTSTSPTLPRPHSPLPGHLGSSPLDSPRNFSPNTPAHFSFASSRRADGRRWSLASLPSSGYGTNTPSSTVSSSCSSQERLHQLPYQPTVDELHFLSKHFGSTESITDEDGGRRSPAVRPRSRSLSPGRSPSSYDNEIVMMNHVYKERFPKATAQMEEKLRDFARAYEPDSVLPLADGVLSFIHHQIIELARDCLTKSRDGLITTVYFYELQENLEKLLQDAYERSESLEVAFVTQLVKKLLIIISRPARLLECLEFNPEEFYHLLEAAEGHAKEGHLVKTDIPRYIIRQLGLTRDPFPDVVRLEEQDSGGSNTPEQDDTSEGRSSTSKAKKPPGESDFDTIKLISNGAYGAVYLVRHRDTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNIGALPVEMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDDILWPEGDEALPTDAQLLISSLLQTNPLVRLGAGGAFEVKQHSFFRDLDWTGLLRQKAEFIPHLESEDDTSYFDTRSDRYHHVNSYDEDDTTEEEPVEIRQFSSCSPRFSKVYSSMEQLSQHEPKTPVSASGASKRDPSAKGPEEKVAGKREGLGGLTLREKTWRGGSPEIKRFSASEASFLEGEASPPLGARRRFSALLEPSRFTAPQEDEDEARLRRPPRPSSDPPSSLDTRVPKEAVQGEGTSTPGEPEATERSHPGDLGPPSKDGDPSGPRATNDLVLRRARHQQLSGDLAVEKRPSRTGGKVIKSASATALSVMIPAVDPHGGSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYGFTLRAIRVYMGDSDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVAVTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKDGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSLSSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASSQSSSPASSTPNSPASSASHHIRPSTLHGLSPKLHRQYRSARCKSAGNIPLSPLAHTPSPTQASPPPLPGHTVGSSHTTQSFPAKLHSSPPVVRPRPKSAEPPRSPLLKRVQSAEKLGASLGADKKGALRKHSLEVGHPDFRKDFHGELALHSLAESDGETPPIEGPGATRQVAVRRLGRQESPLSLGADPLLPDGVQRPMASSKEDSAGGTEACTPPRATTPGSRTLERDSGCTRHQSVQTEDGPGGVARALAKAALSPVQEHETGRRSSSGEAGTPPVPIVVEPARPGVKTQAPQPLGTDSKGLKEPVAQMPLMPDAPRGRERWVLEEVEERTTLSGLRSKPASPKLSPDPQTPTLVPTKNVPRSAAPSVPPASLMVPGTKPEAGLNSRCPAEAVTPAGLTKTGAPSPASLGP

Target ORF information:

RefSeq Version	XM_006531216.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531216.3

ORF Insert Sequence:

ATGGACGATT CTGGCCTGAT CCGCCGCCGG AGGCTGCAGA AGGATTTACC CCTGCCCCGG 
AAAAGCAGCA GTTGCCGTAC CAGCAACCGG AAAAGCCTCA TCCTGACAAG CACTTCTCCG 
ACTCTACCGC GACCCCACTC CCCGCTGCCG GGCCACTTAG GTAGCAGTCC TTTGGACAGC 
CCCCGCAACT TCTCTCCCAA CACTCCCGCC CACTTCTCGT TTGCTTCTTC CCGAAGGGCG 
GATGGACGCC GATGGTCGCT TGCTTCGCTC CCTTCCTCTG GCTATGGCAC CAACACGCCC 
AGCTCAACTG TTTCATCCTC CTGCTCCTCC CAGGAGCGCC TGCATCAACT ACCGTACCAG 
CCCACCGTGG ACGAGCTGCA CTTTCTGTCC AAACACTTCG GCAGCACCGA GAGCATCACC 
GACGAGGATG GTGGCCGCCG CTCTCCCGCC GTGCGTCCTC GCTCTCGCAG CCTCAGCCCC 
GGCCGCTCCC CCTCCTCCTA CGACAACGAA ATTGTGATGA TGAACCATGT GTACAAGGAG 
CGGTTCCCAA AGGCCACTGC ACAGATGGAA GAGAAGCTGC GGGACTTCGC CCGCGCCTAT 
GAACCTGACA GCGTGCTGCC GCTGGCCGAC GGCGTGCTCA GCTTCATCCA CCATCAGATC 
ATTGAACTGG CCCGAGACTG CCTTACCAAG TCCCGCGATG GGCTCATCAC CACGGTCTAT 
TTCTATGAAC TGCAGGAAAA CCTAGAGAAG CTGCTGCAGG ACGCTTATGA ACGCTCGGAG 
AGCTTGGAGG TAGCCTTTGT CACTCAGCTG GTGAAGAAAC TTCTCATAAT CATCTCTCGC 
CCTGCAAGGC TTCTGGAGTG CCTGGAATTC AACCCAGAAG AGTTCTACCA CCTTCTGGAG 
GCAGCCGAGG GACATGCCAA GGAGGGCCAC CTGGTCAAAA CTGACATCCC CCGATACATC 
ATCCGACAGC TGGGCCTCAC TCGGGACCCC TTTCCAGATG TGGTGCGCCT GGAGGAGCAG 
GACAGCGGGG GCTCCAACAC TCCGGAGCAA GACGACACAT CTGAGGGTCG CAGCAGCACC 
TCCAAGGCCA AGAAACCTCC CGGTGAAAGT GACTTTGACA CTATCAAGCT CATTAGCAAT 
GGTGCCTACG GAGCCGTCTA CCTGGTGCGG CACCGGGACA CGAGGCAGCG CTTCGCCATG 
AAGAAGATCA ACAAACAGAA CCTGATCCTG CGCAACCAGA TCCAGCAGGC GTTTGTGGAG 
CGCGACATCC TCACGTTTGC AGAGAACCCA TTTGTGGTCG GCATGTTCTG CTCCTTTGAG 
ACCCGGCGCC ACCTTTGCAT GGTCATGGAG TATGTGGAAG GTGGTGACTG TGCCACTCTC 
CTCAAGAACA TTGGGGCACT GCCTGTGGAG ATGGCGCGAA TGTATTTTGC AGAAACAGTT 
CTAGCCCTTG AGTACTTACA CAATTATGGC ATCGTGCACC GTGACCTCAA GCCAGACAAC 
CTCCTCATTA CTTCCATGGG CCACATCAAG CTCACGGATT TTGGCCTCTC CAAGATGGGA 
CTCATGAGCC TGACCACCAA CTTATATGAG GGGCACATTG AGAAGGATGC CCGGGAGTTC 
CTGGACAAAC AGGTGTGTGG GACCCCAGAG TACATCGCAC CCGAGGTCAT CCTGCGCCAG 
GGCTACGGCA AGCCAGTGGA CTGGTGGGCT ATGGGGATCA TTCTCTACGA GTTCCTGGTG 
GGCTGTGTGC CTTTCTTCGG GGATACCCCA GAAGAGCTCT TTGGACAGGT CATCAGCGAT 
GACATACTGT GGCCTGAAGG GGACGAGGCC CTGCCCACAG ATGCACAGCT TCTAATATCT 
AGCCTCTTGC AGACCAACCC CCTGGTCCGG CTTGGGGCAG GTGGTGCTTT TGAGGTGAAG 
CAGCATAGTT TCTTCCGAGA CCTGGACTGG ACAGGGCTAC TGCGGCAGAA GGCGGAGTTC 
ATCCCCCACC TAGAGTCAGA AGATGACACC AGCTACTTTG ACACCCGCTC CGACAGGTAT 
CACCATGTGA ACTCCTATGA TGAGGACGAC ACCACCGAAG AGGAGCCCGT GGAGATCCGC 
CAATTCTCCT CCTGCTCGCC GCGCTTCAGC AAGGTGTACA GCAGTATGGA GCAGCTGTCT 
CAGCACGAGC CTAAGACCCC CGTCTCAGCA TCTGGGGCCA GCAAGCGGGA CCCAAGCGCT 
AAGGGCCCGG AGGAGAAAGT GGCCGGCAAG AGGGAGGGAC TGGGTGGCCT GACTCTGCGT 
GAGAAGACCT GGAGAGGGGG CTCTCCAGAG ATTAAGCGCT TCTCAGCGTC TGAAGCCAGT 
TTTCTGGAAG GAGAGGCCAG TCCCCCGCTG GGAGCGCGCA GACGTTTCTC AGCACTGCTG 
GAGCCCAGCC GCTTCACTGC CCCTCAAGAG GATGAAGATG AGGCTCGACT CCGCAGACCT 
CCTCGACCCA GCTCCGATCC CCCTAGCTCA CTGGACACAC GCGTCCCCAA AGAGGCAGTT 
CAAGGAGAGG GCACGTCCAC CCCCGGGGAA CCCGAGGCCA CTGAGCGCTC ACATCCAGGT 
GACCTCGGCC CACCCTCCAA GGATGGAGAC CCATCAGGCC CTAGGGCCAC CAACGACCTG 
GTTTTGCGAA GGGCAAGGCA CCAGCAGCTG TCTGGGGATC TTGCTGTGGA GAAGCGACCG 
TCTCGAACTG GGGGCAAAGT TATCAAGTCG GCTTCAGCTA CAGCCTTGTC AGTCATGATT 
CCTGCAGTGG ACCCACATGG AGGCTCACCC CTGGCTAGTC CCATGTCCCC TCGATCTCTC 
TCCTCCAATC CATCCTCACG GGACTCTTCG CCTAGCCGAG ACTACTCACC AGCTGTCAGT 
GGACTCCGTT CCCCCATCAC CATCCAACGC TCAGGCAAGA AATATGGGTT CACACTGCGA 
GCCATCCGTG TCTACATGGG CGACTCCGAT GTCTACAGTG TCCACCACAT TGTTTGGCAC 
GTGGAGGAAG GAGGCCCAGC CCAAGAGGCA GGGCTCTGTG CTGGGGACCT CATCACTCAC 
GTGAATGGTG AGCCCGTGCA TGGTATGGTG CATCCTGAGG TGGTAGAGCT GATCCTGAAG 
AGTGGCAACA AAGTAGCTGT GACCACCACA CCCTTTGAAA ACACTTCCAT CCGGATTGGG 
CCTGCCAGGC GCAGCAGTTA CAAAGCCAAA ATGGCCCGAA GGAACAAAAG GCCTTCGGCC 
AAAGACGGCC AGGAGAGCAA GAAGCGGAGC TCTCTCTTTC GGAAGATCAC AAAGCAGTCG 
AACCTGCTGC ACACCAGTCG ATCGCTGTCC TCTTTGAACC GGTCCTTGTC GTCCAGCGAC 
AGTCTTCCGG GCTCGCCCAC GCACGGACTA CCCGCACGCT CGCCCACACA TAGCTATCGC 
TCTACACCTG ACTCCGCCTA CCTAGGCGCC TCGTCACAGA GTAGCTCGCC AGCTTCCAGC 
ACGCCTAATT CCCCCGCCTC ATCCGCCTCG CACCACATCA GGCCCAGCAC CTTGCACGGG 
CTGTCACCGA AACTACACCG CCAGTACCGC TCAGCGCGCT GTAAATCGGC TGGCAACATC 
CCGCTGTCAC CTCTAGCGCA CACACCATCC CCGACGCAGG CGTCGCCGCC GCCGCTGCCA 
GGCCACACGG TGGGCAGCTC ACATACCACG CAGAGCTTCC CTGCCAAACT ACACTCATCG 
CCACCTGTCG TGCGCCCGCG CCCCAAAAGT GCAGAGCCAC CACGCTCGCC GTTGCTCAAG 
CGCGTGCAGT CAGCGGAGAA ACTAGGGGCT TCGCTGGGAG CAGACAAGAA GGGCGCACTG 
CGCAAACACA GCCTGGAAGT GGGACACCCT GATTTCCGAA AGGACTTCCA TGGTGAGCTG 
GCCCTGCATA GCCTCGCTGA GTCTGATGGT GAGACGCCTC CTATTGAGGG TCCTGGGGCG 
ACCCGGCAGG TCGCCGTGCG ACGCCTGGGT CGCCAGGAAT CACCCCTAAG CTTGGGCGCG 
GACCCGTTGC TACCCGATGG TGTCCAGAGA CCTATGGCGT CAAGCAAGGA GGATTCCGCG 
GGAGGTACAG AGGCTTGCAC CCCACCTCGC GCGACAACCC CTGGGAGCCG GACCCTGGAG 
CGTGACTCGG GTTGCACAAG GCACCAGAGC GTGCAGACTG AGGATGGCCC AGGTGGGGTG 
GCCAGGGCCC TGGCCAAGGC AGCGCTAAGT CCAGTGCAGG AACATGAGAC AGGCCGGCGC 
AGTAGCTCTG GTGAGGCAGG CACACCCCCG GTGCCCATTG TTGTAGAGCC TGCTCGGCCA 
GGGGTCAAGA CCCAGGCACC CCAGCCTCTG GGCACAGACT CCAAGGGATT GAAGGAACCT 
GTAGCCCAGA TGCCTCTGAT GCCTGATGCC CCAAGGGGCA GGGAGCGCTG GGTGCTAGAG 
GAGGTGGAAG AGCGGACCAC GCTAAGCGGA CTTCGCTCTA AGCCCGCCTC CCCCAAACTC 
TCTCCTGACC CCCAGACTCC AACCCTAGTC CCAACAAAGA ATGTACCCCG CAGTGCAGCT 
CCTTCAGTCC CACCTGCTTC CCTCATGGTC CCAGGCACCA AGCCTGAAGC AGGGTTGAAC 
TCCCGGTGTC CTGCTGAAGC TGTGACTCCT GCAGGCCTCA CCAAAACTGG GGCACCTAGC 
CCTGCATCTC TAGGTCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast1 ( XM_006531217.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531280.1 Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X2, mRNA.

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CloneID	OMu41631
Clone ID Related Accession (Same CDS sequence)	XM_006531217.3 , XM_006531217.4
Accession Version	XM_006531217.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531217.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGATT CTGGCCTGAT CCGCCGCCGG AGGCTGCAGG ATTTACCCCT GCCCCGGAAA 
AGCAGCAGTT GCCGTACCAG CAACCGGAAA AGCCTCATCC TGACAAGCAC TTCTCCGACT 
CTACCGCGAC CCCACTCCCC GCTGCCGGGC CACTTAGGTA GCAGTCCTTT GGACAGCCCC 
CGCAACTTCT CTCCCAACAC TCCCGCCCAC TTCTCGTTTG CTTCTTCCCG AAGGGCGGAT 
GGACGCCGAT GGTCGCTTGC TTCGCTCCCT TCCTCTGGCT ATGGCACCAA CACGCCCAGC 
TCAACTGTTT CATCCTCCTG CTCCTCCCAG GAGCGCCTGC ATCAACTACC GTACCAGCCC 
ACCGTGGACG AGCTGCACTT TCTGTCCAAA CACTTCGGCA GCACCGAGAG CATCACCGAC 
GAGGATGGTG GCCGCCGCTC TCCCGCCGTG CGTCCTCGCT CTCGCAGCCT CAGCCCCGGC 
CGCTCCCCCT CCTCCTACGA CAACGAAATT GTGATGATGA ACCATGTGTA CAAGGAGCGG 
TTCCCAAAGG CCACTGCACA GATGGAAGAG AAGCTGCGGG ACTTCGCCCG CGCCTATGAA 
CCTGACAGCG TGCTGCCGCT GGCCGACGGC GTGCTCAGCT TCATCCACCA TCAGATCATT 
GAACTGGCCC GAGACTGCCT TACCAAGTCC CGCGATGGGC TCATCACCAC GGTCTATTTC 
TATGAACTGC AGGAAAACCT AGAGAAGCTG CTGCAGGACG CTTATGAACG CTCGGAGAGC 
TTGGAGGTAG CCTTTGTCAC TCAGCTGGTG AAGAAACTTC TCATAATCAT CTCTCGCCCT 
GCAAGGCTTC TGGAGTGCCT GGAATTCAAC CCAGAAGAGT TCTACCACCT TCTGGAGGCA 
GCCGAGGGAC ATGCCAAGGA GGGCCACCTG GTCAAAACTG ACATCCCCCG ATACATCATC 
CGACAGCTGG GCCTCACTCG GGACCCCTTT CCAGATGTGG TGCGCCTGGA GGAGCAGGAC 
AGCGGGGGCT CCAACACTCC GGAGCAAGAC GACACATCTG AGGGTCGCAG CAGCACCTCC 
AAGGCCAAGA AACCTCCCGG TGAAAGTGAC TTTGACACTA TCAAGCTCAT TAGCAATGGT 
GCCTACGGAG CCGTCTACCT GGTGCGGCAC CGGGACACGA GGCAGCGCTT CGCCATGAAG 
AAGATCAACA AACAGAACCT GATCCTGCGC AACCAGATCC AGCAGGCGTT TGTGGAGCGC 
GACATCCTCA CGTTTGCAGA GAACCCATTT GTGGTCGGCA TGTTCTGCTC CTTTGAGACC 
CGGCGCCACC TTTGCATGGT CATGGAGTAT GTGGAAGGTG GTGACTGTGC CACTCTCCTC 
AAGAACATTG GGGCACTGCC TGTGGAGATG GCGCGAATGT ATTTTGCAGA AACAGTTCTA 
GCCCTTGAGT ACTTACACAA TTATGGCATC GTGCACCGTG ACCTCAAGCC AGACAACCTC 
CTCATTACTT CCATGGGCCA CATCAAGCTC ACGGATTTTG GCCTCTCCAA GATGGGACTC 
ATGAGCCTGA CCACCAACTT ATATGAGGGG CACATTGAGA AGGATGCCCG GGAGTTCCTG 
GACAAACAGG TGTGTGGGAC CCCAGAGTAC ATCGCACCCG AGGTCATCCT GCGCCAGGGC 
TACGGCAAGC CAGTGGACTG GTGGGCTATG GGGATCATTC TCTACGAGTT CCTGGTGGGC 
TGTGTGCCTT TCTTCGGGGA TACCCCAGAA GAGCTCTTTG GACAGGTCAT CAGCGATGAC 
ATACTGTGGC CTGAAGGGGA CGAGGCCCTG CCCACAGATG CACAGCTTCT AATATCTAGC 
CTCTTGCAGA CCAACCCCCT GGTCCGGCTT GGGGCAGGTG GTGCTTTTGA GGTGAAGCAG 
CATAGTTTCT TCCGAGACCT GGACTGGACA GGGCTACTGC GGCAGAAGGC GGAGTTCATC 
CCCCACCTAG AGTCAGAAGA TGACACCAGC TACTTTGACA CCCGCTCCGA CAGGTATCAC 
CATGTGAACT CCTATGATGA GGACGACACC ACCGAAGAGG AGCCCGTGGA GATCCGCCAA 
TTCTCCTCCT GCTCGCCGCG CTTCAGCAAG GTGTACAGCA GTATGGAGCA GCTGTCTCAG 
CACGAGCCTA AGACCCCCGT CTCAGCATCT GGGGCCAGCA AGCGGGACCC AAGCGCTAAG 
GGCCCGGAGG AGAAAGTGGC CGGCAAGAGG GAGGGACTGG GTGGCCTGAC TCTGCGTGAG 
AAGACCTGGA GAGGGGGCTC TCCAGAGATT AAGCGCTTCT CAGCGTCTGA AGCCAGTTTT 
CTGGAAGGAG AGGCCAGTCC CCCGCTGGGA GCGCGCAGAC GTTTCTCAGC ACTGCTGGAG 
CCCAGCCGCT TCACTGCCCC TCAAGAGGAT GAAGATGAGG CTCGACTCCG CAGACCTCCT 
CGACCCAGCT CCGATCCCCC TAGCTCACTG GACACACGCG TCCCCAAAGA GGCAGTTCAA 
GGAGAGGGCA CGTCCACCCC CGGGGAACCC GAGGCCACTG AGCGCTCACA TCCAGGTGAC 
CTCGGCCCAC CCTCCAAGGA TGGAGACCCA TCAGGCCCTA GGGCCACCAA CGACCTGGTT 
TTGCGAAGGG CAAGGCACCA GCAGCTGTCT GGGGATCTTG CTGTGGAGAA GCGACCGTCT 
CGAACTGGGG GCAAAGTTAT CAAGTCGGCT TCAGCTACAG CCTTGTCAGT CATGATTCCT 
GCAGTGGACC CACATGGAGG CTCACCCCTG GCTAGTCCCA TGTCCCCTCG ATCTCTCTCC 
TCCAATCCAT CCTCACGGGA CTCTTCGCCT AGCCGAGACT ACTCACCAGC TGTCAGTGGA 
CTCCGTTCCC CCATCACCAT CCAACGCTCA GGCAAGAAAT ATGGGTTCAC ACTGCGAGCC 
ATCCGTGTCT ACATGGGCGA CTCCGATGTC TACAGTGTCC ACCACATTGT TTGGCACGTG 
GAGGAAGGAG GCCCAGCCCA AGAGGCAGGG CTCTGTGCTG GGGACCTCAT CACTCACGTG 
AATGGTGAGC CCGTGCATGG TATGGTGCAT CCTGAGGTGG TAGAGCTGAT CCTGAAGAGT 
GGCAACAAAG TAGCTGTGAC CACCACACCC TTTGAAAACA CTTCCATCCG GATTGGGCCT 
GCCAGGCGCA GCAGTTACAA AGCCAAAATG GCCCGAAGGA ACAAAAGGCC TTCGGCCAAA 
GACGGCCAGG AGAGCAAGAA GCGGAGCTCT CTCTTTCGGA AGATCACAAA GCAGTCGAAC 
CTGCTGCACA CCAGTCGATC GCTGTCCTCT TTGAACCGGT CCTTGTCGTC CAGCGACAGT 
CTTCCGGGCT CGCCCACGCA CGGACTACCC GCACGCTCGC CCACACATAG CTATCGCTCT 
ACACCTGACT CCGCCTACCT AGGCGCCTCG TCACAGAGTA GCTCGCCAGC TTCCAGCACG 
CCTAATTCCC CCGCCTCATC CGCCTCGCAC CACATCAGGC CCAGCACCTT GCACGGGCTG 
TCACCGAAAC TACACCGCCA GTACCGCTCA GCGCGCTGTA AATCGGCTGG CAACATCCCG 
CTGTCACCTC TAGCGCACAC ACCATCCCCG ACGCAGGCGT CGCCGCCGCC GCTGCCAGGC 
CACACGGTGG GCAGCTCACA TACCACGCAG AGCTTCCCTG CCAAACTACA CTCATCGCCA 
CCTGTCGTGC GCCCGCGCCC CAAAAGTGCA GAGCCACCAC GCTCGCCGTT GCTCAAGCGC 
GTGCAGTCAG CGGAGAAACT AGGGGCTTCG CTGGGAGCAG ACAAGAAGGG CGCACTGCGC 
AAACACAGCC TGGAAGTGGG ACACCCTGAT TTCCGAAAGG ACTTCCATGG TGAGCTGGCC 
CTGCATAGCC TCGCTGAGTC TGATGGTGAG ACGCCTCCTA TTGAGGGTCC TGGGGCGACC 
CGGCAGGTCG CCGTGCGACG CCTGGGTCGC CAGGAATCAC CCCTAAGCTT GGGCGCGGAC 
CCGTTGCTAC CCGATGGTGT CCAGAGACCT ATGGCGTCAA GCAAGGAGGA TTCCGCGGGA 
GGTACAGAGG CTTGCACCCC ACCTCGCGCG ACAACCCCTG GGAGCCGGAC CCTGGAGCGT 
GACTCGGGTT GCACAAGGCA CCAGAGCGTG CAGACTGAGG ATGGCCCAGG TGGGGTGGCC 
AGGGCCCTGG CCAAGGCAGC GCTAAGTCCA GTGCAGGAAC ATGAGACAGG CCGGCGCAGT 
AGCTCTGGTG AGGCAGGCAC ACCCCCGGTG CCCATTGTTG TAGAGCCTGC TCGGCCAGGG 
GTCAAGACCC AGGCACCCCA GCCTCTGGGC ACAGACTCCA AGGGATTGAA GGAACCTGTA 
GCCCAGATGC CTCTGATGCC TGATGCCCCA AGGGGCAGGG AGCGCTGGGT GCTAGAGGAG 
GTGGAAGAGC GGACCACGCT AAGCGGACTT CGCTCTAAGC CCGCCTCCCC CAAACTCTCT 
CCTGACCCCC AGACTCCAAC CCTAGTCCCA ACAAAGAATG TACCCCGCAG TGCAGCTCCT 
TCAGTCCCAC CTGCTTCCCT CATGGTCCCA GGCACCAAGC CTGAAGCAGG GTTGAACTCC 
CGGTGTCCTG CTGAAGCTGT GACTCCTGCA GGCCTCACCA AAACTGGGGC ACCTAGCCCT 
GCATCTCTAG GTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531280.1
CDS	87..4784
Translation	MDDSGLIRRRRLQDLPLPRKSSSCRTSNRKSLILTSTSPTLPRPHSPLPGHLGSSPLDSPRNFSPNTPAHFSFASSRRADGRRWSLASLPSSGYGTNTPSSTVSSSCSSQERLHQLPYQPTVDELHFLSKHFGSTESITDEDGGRRSPAVRPRSRSLSPGRSPSSYDNEIVMMNHVYKERFPKATAQMEEKLRDFARAYEPDSVLPLADGVLSFIHHQIIELARDCLTKSRDGLITTVYFYELQENLEKLLQDAYERSESLEVAFVTQLVKKLLIIISRPARLLECLEFNPEEFYHLLEAAEGHAKEGHLVKTDIPRYIIRQLGLTRDPFPDVVRLEEQDSGGSNTPEQDDTSEGRSSTSKAKKPPGESDFDTIKLISNGAYGAVYLVRHRDTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNIGALPVEMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDDILWPEGDEALPTDAQLLISSLLQTNPLVRLGAGGAFEVKQHSFFRDLDWTGLLRQKAEFIPHLESEDDTSYFDTRSDRYHHVNSYDEDDTTEEEPVEIRQFSSCSPRFSKVYSSMEQLSQHEPKTPVSASGASKRDPSAKGPEEKVAGKREGLGGLTLREKTWRGGSPEIKRFSASEASFLEGEASPPLGARRRFSALLEPSRFTAPQEDEDEARLRRPPRPSSDPPSSLDTRVPKEAVQGEGTSTPGEPEATERSHPGDLGPPSKDGDPSGPRATNDLVLRRARHQQLSGDLAVEKRPSRTGGKVIKSASATALSVMIPAVDPHGGSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYGFTLRAIRVYMGDSDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVAVTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKDGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSLSSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASSQSSSPASSTPNSPASSASHHIRPSTLHGLSPKLHRQYRSARCKSAGNIPLSPLAHTPSPTQASPPPLPGHTVGSSHTTQSFPAKLHSSPPVVRPRPKSAEPPRSPLLKRVQSAEKLGASLGADKKGALRKHSLEVGHPDFRKDFHGELALHSLAESDGETPPIEGPGATRQVAVRRLGRQESPLSLGADPLLPDGVQRPMASSKEDSAGGTEACTPPRATTPGSRTLERDSGCTRHQSVQTEDGPGGVARALAKAALSPVQEHETGRRSSSGEAGTPPVPIVVEPARPGVKTQAPQPLGTDSKGLKEPVAQMPLMPDAPRGRERWVLEEVEERTTLSGLRSKPASPKLSPDPQTPTLVPTKNVPRSAAPSVPPASLMVPGTKPEAGLNSRCPAEAVTPAGLTKTGAPSPASLGP

Target ORF information:

RefSeq Version	XM_006531217.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase 1 (Mast1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531217.3

ORF Insert Sequence:

ATGGACGATT CTGGCCTGAT CCGCCGCCGG AGGCTGCAGG ATTTACCCCT GCCCCGGAAA 
AGCAGCAGTT GCCGTACCAG CAACCGGAAA AGCCTCATCC TGACAAGCAC TTCTCCGACT 
CTACCGCGAC CCCACTCCCC GCTGCCGGGC CACTTAGGTA GCAGTCCTTT GGACAGCCCC 
CGCAACTTCT CTCCCAACAC TCCCGCCCAC TTCTCGTTTG CTTCTTCCCG AAGGGCGGAT 
GGACGCCGAT GGTCGCTTGC TTCGCTCCCT TCCTCTGGCT ATGGCACCAA CACGCCCAGC 
TCAACTGTTT CATCCTCCTG CTCCTCCCAG GAGCGCCTGC ATCAACTACC GTACCAGCCC 
ACCGTGGACG AGCTGCACTT TCTGTCCAAA CACTTCGGCA GCACCGAGAG CATCACCGAC 
GAGGATGGTG GCCGCCGCTC TCCCGCCGTG CGTCCTCGCT CTCGCAGCCT CAGCCCCGGC 
CGCTCCCCCT CCTCCTACGA CAACGAAATT GTGATGATGA ACCATGTGTA CAAGGAGCGG 
TTCCCAAAGG CCACTGCACA GATGGAAGAG AAGCTGCGGG ACTTCGCCCG CGCCTATGAA 
CCTGACAGCG TGCTGCCGCT GGCCGACGGC GTGCTCAGCT TCATCCACCA TCAGATCATT 
GAACTGGCCC GAGACTGCCT TACCAAGTCC CGCGATGGGC TCATCACCAC GGTCTATTTC 
TATGAACTGC AGGAAAACCT AGAGAAGCTG CTGCAGGACG CTTATGAACG CTCGGAGAGC 
TTGGAGGTAG CCTTTGTCAC TCAGCTGGTG AAGAAACTTC TCATAATCAT CTCTCGCCCT 
GCAAGGCTTC TGGAGTGCCT GGAATTCAAC CCAGAAGAGT TCTACCACCT TCTGGAGGCA 
GCCGAGGGAC ATGCCAAGGA GGGCCACCTG GTCAAAACTG ACATCCCCCG ATACATCATC 
CGACAGCTGG GCCTCACTCG GGACCCCTTT CCAGATGTGG TGCGCCTGGA GGAGCAGGAC 
AGCGGGGGCT CCAACACTCC GGAGCAAGAC GACACATCTG AGGGTCGCAG CAGCACCTCC 
AAGGCCAAGA AACCTCCCGG TGAAAGTGAC TTTGACACTA TCAAGCTCAT TAGCAATGGT 
GCCTACGGAG CCGTCTACCT GGTGCGGCAC CGGGACACGA GGCAGCGCTT CGCCATGAAG 
AAGATCAACA AACAGAACCT GATCCTGCGC AACCAGATCC AGCAGGCGTT TGTGGAGCGC 
GACATCCTCA CGTTTGCAGA GAACCCATTT GTGGTCGGCA TGTTCTGCTC CTTTGAGACC 
CGGCGCCACC TTTGCATGGT CATGGAGTAT GTGGAAGGTG GTGACTGTGC CACTCTCCTC 
AAGAACATTG GGGCACTGCC TGTGGAGATG GCGCGAATGT ATTTTGCAGA AACAGTTCTA 
GCCCTTGAGT ACTTACACAA TTATGGCATC GTGCACCGTG ACCTCAAGCC AGACAACCTC 
CTCATTACTT CCATGGGCCA CATCAAGCTC ACGGATTTTG GCCTCTCCAA GATGGGACTC 
ATGAGCCTGA CCACCAACTT ATATGAGGGG CACATTGAGA AGGATGCCCG GGAGTTCCTG 
GACAAACAGG TGTGTGGGAC CCCAGAGTAC ATCGCACCCG AGGTCATCCT GCGCCAGGGC 
TACGGCAAGC CAGTGGACTG GTGGGCTATG GGGATCATTC TCTACGAGTT CCTGGTGGGC 
TGTGTGCCTT TCTTCGGGGA TACCCCAGAA GAGCTCTTTG GACAGGTCAT CAGCGATGAC 
ATACTGTGGC CTGAAGGGGA CGAGGCCCTG CCCACAGATG CACAGCTTCT AATATCTAGC 
CTCTTGCAGA CCAACCCCCT GGTCCGGCTT GGGGCAGGTG GTGCTTTTGA GGTGAAGCAG 
CATAGTTTCT TCCGAGACCT GGACTGGACA GGGCTACTGC GGCAGAAGGC GGAGTTCATC 
CCCCACCTAG AGTCAGAAGA TGACACCAGC TACTTTGACA CCCGCTCCGA CAGGTATCAC 
CATGTGAACT CCTATGATGA GGACGACACC ACCGAAGAGG AGCCCGTGGA GATCCGCCAA 
TTCTCCTCCT GCTCGCCGCG CTTCAGCAAG GTGTACAGCA GTATGGAGCA GCTGTCTCAG 
CACGAGCCTA AGACCCCCGT CTCAGCATCT GGGGCCAGCA AGCGGGACCC AAGCGCTAAG 
GGCCCGGAGG AGAAAGTGGC CGGCAAGAGG GAGGGACTGG GTGGCCTGAC TCTGCGTGAG 
AAGACCTGGA GAGGGGGCTC TCCAGAGATT AAGCGCTTCT CAGCGTCTGA AGCCAGTTTT 
CTGGAAGGAG AGGCCAGTCC CCCGCTGGGA GCGCGCAGAC GTTTCTCAGC ACTGCTGGAG 
CCCAGCCGCT TCACTGCCCC TCAAGAGGAT GAAGATGAGG CTCGACTCCG CAGACCTCCT 
CGACCCAGCT CCGATCCCCC TAGCTCACTG GACACACGCG TCCCCAAAGA GGCAGTTCAA 
GGAGAGGGCA CGTCCACCCC CGGGGAACCC GAGGCCACTG AGCGCTCACA TCCAGGTGAC 
CTCGGCCCAC CCTCCAAGGA TGGAGACCCA TCAGGCCCTA GGGCCACCAA CGACCTGGTT 
TTGCGAAGGG CAAGGCACCA GCAGCTGTCT GGGGATCTTG CTGTGGAGAA GCGACCGTCT 
CGAACTGGGG GCAAAGTTAT CAAGTCGGCT TCAGCTACAG CCTTGTCAGT CATGATTCCT 
GCAGTGGACC CACATGGAGG CTCACCCCTG GCTAGTCCCA TGTCCCCTCG ATCTCTCTCC 
TCCAATCCAT CCTCACGGGA CTCTTCGCCT AGCCGAGACT ACTCACCAGC TGTCAGTGGA 
CTCCGTTCCC CCATCACCAT CCAACGCTCA GGCAAGAAAT ATGGGTTCAC ACTGCGAGCC 
ATCCGTGTCT ACATGGGCGA CTCCGATGTC TACAGTGTCC ACCACATTGT TTGGCACGTG 
GAGGAAGGAG GCCCAGCCCA AGAGGCAGGG CTCTGTGCTG GGGACCTCAT CACTCACGTG 
AATGGTGAGC CCGTGCATGG TATGGTGCAT CCTGAGGTGG TAGAGCTGAT CCTGAAGAGT 
GGCAACAAAG TAGCTGTGAC CACCACACCC TTTGAAAACA CTTCCATCCG GATTGGGCCT 
GCCAGGCGCA GCAGTTACAA AGCCAAAATG GCCCGAAGGA ACAAAAGGCC TTCGGCCAAA 
GACGGCCAGG AGAGCAAGAA GCGGAGCTCT CTCTTTCGGA AGATCACAAA GCAGTCGAAC 
CTGCTGCACA CCAGTCGATC GCTGTCCTCT TTGAACCGGT CCTTGTCGTC CAGCGACAGT 
CTTCCGGGCT CGCCCACGCA CGGACTACCC GCACGCTCGC CCACACATAG CTATCGCTCT 
ACACCTGACT CCGCCTACCT AGGCGCCTCG TCACAGAGTA GCTCGCCAGC TTCCAGCACG 
CCTAATTCCC CCGCCTCATC CGCCTCGCAC CACATCAGGC CCAGCACCTT GCACGGGCTG 
TCACCGAAAC TACACCGCCA GTACCGCTCA GCGCGCTGTA AATCGGCTGG CAACATCCCG 
CTGTCACCTC TAGCGCACAC ACCATCCCCG ACGCAGGCGT CGCCGCCGCC GCTGCCAGGC 
CACACGGTGG GCAGCTCACA TACCACGCAG AGCTTCCCTG CCAAACTACA CTCATCGCCA 
CCTGTCGTGC GCCCGCGCCC CAAAAGTGCA GAGCCACCAC GCTCGCCGTT GCTCAAGCGC 
GTGCAGTCAG CGGAGAAACT AGGGGCTTCG CTGGGAGCAG ACAAGAAGGG CGCACTGCGC 
AAACACAGCC TGGAAGTGGG ACACCCTGAT TTCCGAAAGG ACTTCCATGG TGAGCTGGCC 
CTGCATAGCC TCGCTGAGTC TGATGGTGAG ACGCCTCCTA TTGAGGGTCC TGGGGCGACC 
CGGCAGGTCG CCGTGCGACG CCTGGGTCGC CAGGAATCAC CCCTAAGCTT GGGCGCGGAC 
CCGTTGCTAC CCGATGGTGT CCAGAGACCT ATGGCGTCAA GCAAGGAGGA TTCCGCGGGA 
GGTACAGAGG CTTGCACCCC ACCTCGCGCG ACAACCCCTG GGAGCCGGAC CCTGGAGCGT 
GACTCGGGTT GCACAAGGCA CCAGAGCGTG CAGACTGAGG ATGGCCCAGG TGGGGTGGCC 
AGGGCCCTGG CCAAGGCAGC GCTAAGTCCA GTGCAGGAAC ATGAGACAGG CCGGCGCAGT 
AGCTCTGGTG AGGCAGGCAC ACCCCCGGTG CCCATTGTTG TAGAGCCTGC TCGGCCAGGG 
GTCAAGACCC AGGCACCCCA GCCTCTGGGC ACAGACTCCA AGGGATTGAA GGAACCTGTA 
GCCCAGATGC CTCTGATGCC TGATGCCCCA AGGGGCAGGG AGCGCTGGGT GCTAGAGGAG 
GTGGAAGAGC GGACCACGCT AAGCGGACTT CGCTCTAAGC CCGCCTCCCC CAAACTCTCT 
CCTGACCCCC AGACTCCAAC CCTAGTCCCA ACAAAGAATG TACCCCGCAG TGCAGCTCCT 
TCAGTCCCAC CTGCTTCCCT CATGGTCCCA GGCACCAAGC CTGAAGCAGG GTTGAACTCC 
CGGTGTCCTG CTGAAGCTGT GACTCCTGCA GGCCTCACCA AAACTGGGGC ACCTAGCCCT 
GCATCTCTAG GTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases.
The Journal of biological chemistry280(32)28936-43(2005 Aug)
Valiente M,Andr?s-Pons A,Gomar B,Torres J,Gil A,Tapparel C,Antonarakis SE,Pulido R

Genomic analysis of mouse retinal development.
PLoS biology2(9)E247(2004 Sep)
Blackshaw S,Harpavat S,Trimarchi J,Cai L,Huang H,Kuo WP,Weber G,Lee K,Fraioli RE,Cho SH,Yung R,Asch E,Ohno-Machado L,Wong WH,Cepko CL

Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes10(1)35-48(2003 Feb)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Aizawa H,Yuasa S,Nakajima D,Nagase T,Ohara O,Koga H

Deoxyribonuclease II: structure and chromosomal localization of the murine gene, and comparison with the genomic structure of the human and three C. elegans homologs.
Gene252(1-2)155-62(2000 Jul)
Krieser RJ,Eastman A

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Mast1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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