Cldn18 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cldn18
Entrez Gene ID	56492
Full Name	claudin 18
General protein information	Preferred Names claudin 18 Names claudin-18
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 E3.3
Summary	This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is a downstream target gene regulated by the T/EBP/NKX2.1 homeodomain transcription factor. Four alternatively spliced transcript variants resulted from alternative promoters and alternative splicing have been identified, which encode two lung-specific isoforms and two stomach-specific isoforms respectively. This gene is also expressed in colons, inner ear and skin, and its expression is increased in both experimental colitis and ulcerative colitis. [provided by RefSeq, Aug 2010].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001194921.1	NP_001181850.1	claudin-18 isoform A2.1
NM_001194923.1	NP_001181852.1	claudin-18 isoform A2.2
NM_019815.3	NP_062789.1	claudin-18 isoform A1.1 precursor
NM_001194922.1	NP_001181851.1	claudin-18 isoform A1.2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLDN18	XP_001155235.1
	CLDN18	XP_001114708.1
Bos taurus (cattle)	CLDN18	NP_001068984.1
Danio rerio (zebrafish)	cldn18	XP_002660862.1
Xenopus tropicalis (tropical clawed frog)	cldn18	XP_002938162.1
Homo sapiens (human)	CLDN18	NP_001002026.1
Canis lupus familiaris (dog)	CLDN18	XP_852057.1
Gallus gallus (chicken)	CLDN18	XP_003641778.1
Rattus norvegicus (Norway rat)	Cldn18	NP_001014118.1
Mus musculus (house mouse)	Cldn18	NP_001181850.1

Gene Ontology
Bibliography

Related articles in PubMed

Claudin18 associated with corticosteroid-induced expression of surfactant proteins in pulmonary epithelial cells.
Shi F, Liao Y, Dong Y, Wang Y, Xie Y, Wan H
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians1-6(2017 Oct)

Transitional basal cells at the squamous-columnar junction generate Barrett's oesophagus.
Jiang M, Li H, Zhang Y, Yang Y, Lu R, Liu K, Lin S, Lan X, Wang H, Wu H, Zhu J, Zhou Z, Xu J, Lee DK, Zhang L, Lee YC, Yuan J, Abrams JA, Wang TC, Sepulveda AR, Wu Q, Chen H, Sun X, She J, Chen X, Que J
Nature550(7677)529-533(2017 Oct)

Genetic ablation of carbonic anhydrase IX disrupts gastric barrier function via claudin-18 downregulation and acid backflux.
Li T, Liu X, Riederer B, Nikolovska K, Singh AK, M?kel? KA, Seidler A, Liu Y, Gros G, Bartels H, Herzig KH, Seidler U
Acta physiologica (Oxford, England)(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The presented data substantiate the hypothesis that claudin-18 is a central barrier-forming component of tight junctions and show that IL-13 downregulates claudin-18. These data also suggest that the loss of claudin-18 is associated with increased sensitization to aeroantigens and airway responsiveness
Title: Claudin-18 deficiency is associated with airway epithelial barrier dysfunction and asthma.

The claudin-18 knockout mice exhibited decreased P2X7 mRNA transcript abundance as measured by mRNA expression microarray
Title: P2X7R-dependent regulation of glycogen synthase kinase 3? and claudin-18 in alveolar epithelial type I cells of mice lung.

CLDN18 deficiency results in epithelial barrier dysfunction, injury, and impaired alveolarization in mice.
Title: Claudin-18 deficiency results in alveolar barrier dysfunction and impaired alveologenesis in mice.

Identify a role for claudin 18 in alveolar fluid homeostasis beyond its direct contributions to barrier properties.
Title: Knockout mice reveal key roles for claudin 18 in alveolar barrier properties and fluid homeostasis.

we conclude that the estrogen effects on osteoclasts may in part be mediated via regulation of Cldn-18 signaling
Title: Disruption of claudin-18 diminishes ovariectomy-induced bone loss in mice.

The following Cldn18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cldn18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13461	NM_001194922.1 Latest version!	Mus musculus claudin 18 (Cldn18), transcript variant A1.2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu13960	NM_001194923.1 Latest version!	Mus musculus claudin 18 (Cldn18), transcript variant A2.2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu13488	NM_019815.3 Latest version!	Mus musculus claudin 18 (Cldn18), transcript variant A1.1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu13624	NM_001194921.1 Latest version!	Mus musculus claudin 18 (Cldn18), transcript variant A2.1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cldn18 ( NM_001194922.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001181851.1 Mus musculus claudin 18 (Cldn18), transcript variant A1.2, mRNA.

Price & Availability↑

CloneID	OMu13461
Clone ID Related Accession (Same CDS sequence)	NM_001194922.1
Accession Version	NM_001194922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	claudin-18 isoform A1.2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB663682.1, AK033657.1, AF349450.1 and AC157949.1. Transcript Variant: This variant (A1.2) is the lung-specific form. It has an additional segment in the CDS, which results in an immediate translation termination, as compared to variant A1.1. The resulting isoform (A1.2) is C-terminal truncated, as compared to isoform A1.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF349450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACCA CCACGTGCCA GGTGGTAGGG CTTCTCCTGT CCCTCCTGGG TCTGGCCGGC 
TGCATAGCCG CCACTGGGAT GGACATGTGG AGCACTCAAG ACCTGTATGA CAACCCAGTC 
ACCGCCGTGT TCCAGTATGA AGGGCTCTGG AGGAGTTGCG TGCAACAGAG CTCGGGGTTC 
ACCGAGTGCC GGCCATACTT CACCATCCTG GGCCTTCCAG CCATGCTGCA AGCTGTACGA 
GCCCTGATGA TCGTGGGCAT TGTTCTGGGG GTCATCGGTA TCCTCGTGTC CATCTTCGCC 
CTGAAGTGCA TTCGCATTGG TAGCATGGAT GACTCTGCCA AGGCCAAGAT GACTCTGACT 
TCTGGGATCT TGTTCATCAT CTCCGGCATC TGTGCAATCA TTGGTGTGTC TGTGTTTGCC 
AACATGCTGG TGACCAACTT CTGGATGTCC ACAGCTAACA TGTACAGCGG CATGGGCGGC 
ATGGGTGGCA TGGTGCAGAC CGTTCAGACC AGGTACACCT TTGGTGCAGC TCTGTTCGTG 
GGCTGGGTTG CTGGAGGCCT CACCCTGATT GGGGGAGTGA TGATGTGCAT CGCCTGCCGT 
GGCCTGACAC CAGATGACAG CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001181851.1
CDS	116..742
Translation	MATTTCQVVGLLLSLLGLAGCIAATGMDMWSTQDLYDNPVTAVFQYEGLWRSCVQQSSGFTECRPYFTILGLPAMLQAVRALMIVGIVLGVIGILVSIFALKCIRIGSMDDSAKAKMTLTSGILFIISGICAIIGVSVFANMLVTNFWMSTANMYSGMGGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLTPDDSK

Target ORF information:

RefSeq Version	NM_001194922.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus claudin 18 (Cldn18), transcript variant A1.2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001194922.1

ORF Insert Sequence:

ATGGCCACCA CCACGTGCCA GGTGGTAGGG CTTCTCCTGT CCCTCCTGGG TCTGGCCGGC 
TGCATAGCCG CCACTGGGAT GGACATGTGG AGCACTCAAG ACCTGTATGA CAACCCAGTC 
ACCGCCGTGT TCCAGTATGA AGGGCTCTGG AGGAGTTGCG TGCAACAGAG CTCGGGGTTC 
ACCGAGTGCC GGCCATACTT CACCATCCTG GGCCTTCCAG CCATGCTGCA AGCTGTACGA 
GCCCTGATGA TCGTGGGCAT TGTTCTGGGG GTCATCGGTA TCCTCGTGTC CATCTTCGCC 
CTGAAGTGCA TTCGCATTGG TAGCATGGAT GACTCTGCCA AGGCCAAGAT GACTCTGACT 
TCTGGGATCT TGTTCATCAT CTCCGGCATC TGTGCAATCA TTGGTGTGTC TGTGTTTGCC 
AACATGCTGG TGACCAACTT CTGGATGTCC ACAGCTAACA TGTACAGCGG CATGGGCGGC 
ATGGGTGGCA TGGTGCAGAC CGTTCAGACC AGGTACACCT TTGGTGCAGC TCTGTTCGTG 
GGCTGGGTTG CTGGAGGCCT CACCCTGATT GGGGGAGTGA TGATGTGCAT CGCCTGCCGT 
GGCCTGACAC CAGATGACAG CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cldn18 ( NM_001194923.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001181852.1 Mus musculus claudin 18 (Cldn18), transcript variant A2.2, mRNA.

Price & Availability↑

CloneID	OMu13960
Clone ID Related Accession (Same CDS sequence)	NM_001194923.1
Accession Version	NM_001194923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	claudin-18 isoform A2.2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF349453.1, AA028634.1 and AC157949.1. Transcript Variant: This variant (A2.2) is the stomach-specific form. It has an alternate 5' exon, and an additional segment in the CDS, which results in an immediate translation termination, as compared to variant A1.1. The resulting isoform (A2.2) has a different N-terminus and is C-terminal truncated, as compared to isoform A1.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF349453.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGTGA CCGCCTGCCA GGGCTTGGGG TTTGTGGTGT CACTGATCGG GTTTGCGGGC 
ATCATTGCAG CCACTTGTAT GGACCAGTGG AGCACCCAGG ATTTATACAA CAACCCGGTG 
ACCGCTGTAT TCAACTACCA AGGGCTATGG CGTTCATGCG TCCGAGAGAG CTCTGGCTTC 
ACCGAGTGCC GAGGCTACTT CACCCTGTTG GGGTTGCCAG CCATGCTGCA AGCTGTACGA 
GCCCTGATGA TCGTGGGCAT TGTTCTGGGG GTCATCGGTA TCCTCGTGTC CATCTTCGCC 
CTGAAGTGCA TTCGCATTGG TAGCATGGAT GACTCTGCCA AGGCCAAGAT GACTCTGACT 
TCTGGGATCT TGTTCATCAT CTCCGGCATC TGTGCAATCA TTGGTGTGTC TGTGTTTGCC 
AACATGCTGG TGACCAACTT CTGGATGTCC ACAGCTAACA TGTACAGCGG CATGGGCGGC 
ATGGGTGGCA TGGTGCAGAC CGTTCAGACC AGGTACACCT TTGGTGCAGC TCTGTTCGTG 
GGCTGGGTTG CTGGAGGCCT CACCCTGATT GGGGGAGTGA TGATGTGCAT CGCCTGCCGT 
GGCCTGACAC CAGATGACAG CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001181852.1
CDS	53..679
Translation	MSVTACQGLGFVVSLIGFAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGVIGILVSIFALKCIRIGSMDDSAKAKMTLTSGILFIISGICAIIGVSVFANMLVTNFWMSTANMYSGMGGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLTPDDSK

Target ORF information:

RefSeq Version	NM_001194923.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus claudin 18 (Cldn18), transcript variant A2.2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001194923.1

ORF Insert Sequence:

ATGTCGGTGA CCGCCTGCCA GGGCTTGGGG TTTGTGGTGT CACTGATCGG GTTTGCGGGC 
ATCATTGCAG CCACTTGTAT GGACCAGTGG AGCACCCAGG ATTTATACAA CAACCCGGTG 
ACCGCTGTAT TCAACTACCA AGGGCTATGG CGTTCATGCG TCCGAGAGAG CTCTGGCTTC 
ACCGAGTGCC GAGGCTACTT CACCCTGTTG GGGTTGCCAG CCATGCTGCA AGCTGTACGA 
GCCCTGATGA TCGTGGGCAT TGTTCTGGGG GTCATCGGTA TCCTCGTGTC CATCTTCGCC 
CTGAAGTGCA TTCGCATTGG TAGCATGGAT GACTCTGCCA AGGCCAAGAT GACTCTGACT 
TCTGGGATCT TGTTCATCAT CTCCGGCATC TGTGCAATCA TTGGTGTGTC TGTGTTTGCC 
AACATGCTGG TGACCAACTT CTGGATGTCC ACAGCTAACA TGTACAGCGG CATGGGCGGC 
ATGGGTGGCA TGGTGCAGAC CGTTCAGACC AGGTACACCT TTGGTGCAGC TCTGTTCGTG 
GGCTGGGTTG CTGGAGGCCT CACCCTGATT GGGGGAGTGA TGATGTGCAT CGCCTGCCGT 
GGCCTGACAC CAGATGACAG CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cldn18 ( NM_019815.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062789.1 Mus musculus claudin 18 (Cldn18), transcript variant A1.1, mRNA.

Price & Availability↑

CloneID	OMu13488
Clone ID Related Accession (Same CDS sequence)	NM_019815.3
Accession Version	NM_019815.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	claudin-18 isoform A1.1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB663682.1, BX528145.1, AF221068.1, AC157949.1 and CF805431.1. On Aug 13, 2010 this sequence version replaced NM_019815.2. Transcript Variant: This variant (A1.1) is the lung-specific form. It encodes the longer isoform (A1.1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF221068.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACCA CCACGTGCCA GGTGGTAGGG CTTCTCCTGT CCCTCCTGGG TCTGGCCGGC 
TGCATAGCCG CCACTGGGAT GGACATGTGG AGCACTCAAG ACCTGTATGA CAACCCAGTC 
ACCGCCGTGT TCCAGTATGA AGGGCTCTGG AGGAGTTGCG TGCAACAGAG CTCGGGGTTC 
ACCGAGTGCC GGCCATACTT CACCATCCTG GGCCTTCCAG CCATGCTGCA AGCTGTACGA 
GCCCTGATGA TCGTGGGCAT TGTTCTGGGG GTCATCGGTA TCCTCGTGTC CATCTTCGCC 
CTGAAGTGCA TTCGCATTGG TAGCATGGAT GACTCTGCCA AGGCCAAGAT GACTCTGACT 
TCTGGGATCT TGTTCATCAT CTCCGGCATC TGTGCAATCA TTGGTGTGTC TGTGTTTGCC 
AACATGCTGG TGACCAACTT CTGGATGTCC ACAGCTAACA TGTACAGCGG CATGGGCGGC 
ATGGGTGGCA TGGTGCAGAC CGTTCAGACC AGGTACACCT TTGGTGCAGC TCTGTTCGTG 
GGCTGGGTTG CTGGAGGCCT CACCCTGATT GGGGGAGTGA TGATGTGCAT CGCCTGCCGT 
GGCCTGACAC CAGATGACAG CAACTTCAAA GCTGTGTCTT ACCATGCCTC TGGCCAAAAT 
GTTGCCTACA GGCCTGGAGG CTTTAAGGCC AGCACTGGCT TTGGGTCCAA CACCAGAAAC 
AAGAAGATCT ACGATGGGGG TGCCCGCACA GAAGACGATG AACAGTCTCA TCCTACCAAG 
TATGACTATG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062789.1
CDS	116..910
Translation	MATTTCQVVGLLLSLLGLAGCIAATGMDMWSTQDLYDNPVTAVFQYEGLWRSCVQQSSGFTECRPYFTILGLPAMLQAVRALMIVGIVLGVIGILVSIFALKCIRIGSMDDSAKAKMTLTSGILFIISGICAIIGVSVFANMLVTNFWMSTANMYSGMGGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLTPDDSNFKAVSYHASGQNVAYRPGGFKASTGFGSNTRNKKIYDGGARTEDDEQSHPTKYDYV

Target ORF information:

RefSeq Version	NM_019815.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus claudin 18 (Cldn18), transcript variant A1.1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019815.3

ORF Insert Sequence:

ATGGCCACCA CCACGTGCCA GGTGGTAGGG CTTCTCCTGT CCCTCCTGGG TCTGGCCGGC 
TGCATAGCCG CCACTGGGAT GGACATGTGG AGCACTCAAG ACCTGTATGA CAACCCAGTC 
ACCGCCGTGT TCCAGTATGA AGGGCTCTGG AGGAGTTGCG TGCAACAGAG CTCGGGGTTC 
ACCGAGTGCC GGCCATACTT CACCATCCTG GGCCTTCCAG CCATGCTGCA AGCTGTACGA 
GCCCTGATGA TCGTGGGCAT TGTTCTGGGG GTCATCGGTA TCCTCGTGTC CATCTTCGCC 
CTGAAGTGCA TTCGCATTGG TAGCATGGAT GACTCTGCCA AGGCCAAGAT GACTCTGACT 
TCTGGGATCT TGTTCATCAT CTCCGGCATC TGTGCAATCA TTGGTGTGTC TGTGTTTGCC 
AACATGCTGG TGACCAACTT CTGGATGTCC ACAGCTAACA TGTACAGCGG CATGGGCGGC 
ATGGGTGGCA TGGTGCAGAC CGTTCAGACC AGGTACACCT TTGGTGCAGC TCTGTTCGTG 
GGCTGGGTTG CTGGAGGCCT CACCCTGATT GGGGGAGTGA TGATGTGCAT CGCCTGCCGT 
GGCCTGACAC CAGATGACAG CAACTTCAAA GCTGTGTCTT ACCATGCCTC TGGCCAAAAT 
GTTGCCTACA GGCCTGGAGG CTTTAAGGCC AGCACTGGCT TTGGGTCCAA CACCAGAAAC 
AAGAAGATCT ACGATGGGGG TGCCCGCACA GAAGACGATG AACAGTCTCA TCCTACCAAG 
TATGACTATG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cldn18 ( NM_001194921.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001181850.1 Mus musculus claudin 18 (Cldn18), transcript variant A2.1, mRNA.

Price & Availability↑

CloneID	OMu13624
Clone ID Related Accession (Same CDS sequence)	NM_001194921.1
Accession Version	NM_001194921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	claudin-18 isoform A2.1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK033657.1, AF349451.1 and AC157949.1. Transcript Variant: This variant (A2.1) is the stomach-specific form. It has an alternate 5' exon, as compared to variant A1.1. The resulting isoform (A2.1) is the same size but has a different N-terminus, as compared to isoform A1.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK033657.1, AF349451.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGTGA CCGCCTGCCA GGGCTTGGGG TTTGTGGTGT CACTGATCGG GTTTGCGGGC 
ATCATTGCAG CCACTTGTAT GGACCAGTGG AGCACCCAGG ATTTATACAA CAACCCGGTG 
ACCGCTGTAT TCAACTACCA AGGGCTATGG CGTTCATGCG TCCGAGAGAG CTCTGGCTTC 
ACCGAGTGCC GAGGCTACTT CACCCTGTTG GGGTTGCCAG CCATGCTGCA AGCTGTACGA 
GCCCTGATGA TCGTGGGCAT TGTTCTGGGG GTCATCGGTA TCCTCGTGTC CATCTTCGCC 
CTGAAGTGCA TTCGCATTGG TAGCATGGAT GACTCTGCCA AGGCCAAGAT GACTCTGACT 
TCTGGGATCT TGTTCATCAT CTCCGGCATC TGTGCAATCA TTGGTGTGTC TGTGTTTGCC 
AACATGCTGG TGACCAACTT CTGGATGTCC ACAGCTAACA TGTACAGCGG CATGGGCGGC 
ATGGGTGGCA TGGTGCAGAC CGTTCAGACC AGGTACACCT TTGGTGCAGC TCTGTTCGTG 
GGCTGGGTTG CTGGAGGCCT CACCCTGATT GGGGGAGTGA TGATGTGCAT CGCCTGCCGT 
GGCCTGACAC CAGATGACAG CAACTTCAAA GCTGTGTCTT ACCATGCCTC TGGCCAAAAT 
GTTGCCTACA GGCCTGGAGG CTTTAAGGCC AGCACTGGCT TTGGGTCCAA CACCAGAAAC 
AAGAAGATCT ACGATGGGGG TGCCCGCACA GAAGACGATG AACAGTCTCA TCCTACCAAG 
TATGACTATG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001181850.1
CDS	53..847
Translation	MSVTACQGLGFVVSLIGFAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGVIGILVSIFALKCIRIGSMDDSAKAKMTLTSGILFIISGICAIIGVSVFANMLVTNFWMSTANMYSGMGGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLTPDDSNFKAVSYHASGQNVAYRPGGFKASTGFGSNTRNKKIYDGGARTEDDEQSHPTKYDYV

Target ORF information:

RefSeq Version	NM_001194921.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus claudin 18 (Cldn18), transcript variant A2.1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001194921.1

ORF Insert Sequence:

ATGTCGGTGA CCGCCTGCCA GGGCTTGGGG TTTGTGGTGT CACTGATCGG GTTTGCGGGC 
ATCATTGCAG CCACTTGTAT GGACCAGTGG AGCACCCAGG ATTTATACAA CAACCCGGTG 
ACCGCTGTAT TCAACTACCA AGGGCTATGG CGTTCATGCG TCCGAGAGAG CTCTGGCTTC 
ACCGAGTGCC GAGGCTACTT CACCCTGTTG GGGTTGCCAG CCATGCTGCA AGCTGTACGA 
GCCCTGATGA TCGTGGGCAT TGTTCTGGGG GTCATCGGTA TCCTCGTGTC CATCTTCGCC 
CTGAAGTGCA TTCGCATTGG TAGCATGGAT GACTCTGCCA AGGCCAAGAT GACTCTGACT 
TCTGGGATCT TGTTCATCAT CTCCGGCATC TGTGCAATCA TTGGTGTGTC TGTGTTTGCC 
AACATGCTGG TGACCAACTT CTGGATGTCC ACAGCTAACA TGTACAGCGG CATGGGCGGC 
ATGGGTGGCA TGGTGCAGAC CGTTCAGACC AGGTACACCT TTGGTGCAGC TCTGTTCGTG 
GGCTGGGTTG CTGGAGGCCT CACCCTGATT GGGGGAGTGA TGATGTGCAT CGCCTGCCGT 
GGCCTGACAC CAGATGACAG CAACTTCAAA GCTGTGTCTT ACCATGCCTC TGGCCAAAAT 
GTTGCCTACA GGCCTGGAGG CTTTAAGGCC AGCACTGGCT TTGGGTCCAA CACCAGAAAC 
AAGAAGATCT ACGATGGGGG TGCCCGCACA GAAGACGATG AACAGTCTCA TCCTACCAAG 
TATGACTATG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Claudin18 associated with corticosteroid-induced expression of surfactant proteins in pulmonary epithelial cells.
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians1-6(2017 Oct)
Shi F,Liao Y,Dong Y,Wang Y,Xie Y,Wan H

Transitional basal cells at the squamous-columnar junction generate Barrett's oesophagus.
Nature550(7677)529-533(2017 Oct)
Jiang M,Li H,Zhang Y,Yang Y,Lu R,Liu K,Lin S,Lan X,Wang H,Wu H,Zhu J,Zhou Z,Xu J,Lee DK,Zhang L,Lee YC,Yuan J,Abrams JA,Wang TC,Sepulveda AR,Wu Q,Chen H,Sun X,She J,Chen X,Que J

Genetic ablation of carbonic anhydrase IX disrupts gastric barrier function via claudin-18 downregulation and acid backflux.
Acta physiologica (Oxford, England)(2017 Jul)
Li T,Liu X,Riederer B,Nikolovska K,Singh AK,M?kel? KA,Seidler A,Liu Y,Gros G,Bartels H,Herzig KH,Seidler U

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Cldn18 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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