Rbx1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rbx1
Entrez Gene ID	56438
Full Name	ring-box 1
Synonyms	1500002P15Rik,AA517855,ROC1
General protein information	Preferred Names ring-box 1 Names E3 ubiquitin-protein ligase RBX1 RING finger protein 75 ring-box protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_019712.3	NP_062686.1	E3 ubiquitin-protein ligase RBX1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC780968	XP_002686877.2
Arabidopsis thaliana (thale cress)	AT3G42830	NP_189869.1
Mus musculus (house mouse)	Rbx1	NP_062686.1
Rattus norvegicus (Norway rat)	Rbx1	NP_001029307.1
Oryza sativa (rice)	Os01g0106800	NP_001041779.1

Gene Ontology
Bibliography

Related articles in PubMed

CUL9 mediates the functions of the 3M complex and ubiquitylates survivin to maintain genome integrity.
Li Z, Pei XH, Yan J, Yan F, Cappell KM, Whitehurst AW, Xiong Y
Molecular cell54(5)805-19(2014 Jun)

CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.
Yu C, Zhang YL, Pan WW, Li XM, Wang ZW, Ge ZJ, Zhou JJ, Cang Y, Tong C, Sun QY, Fan HY
Science (New York, N.Y.)342(6165)1518-21(2013 Dec)

Rbx2 regulates neuronal migration through different cullin 5-RING ligase adaptors.
Sim? S, Cooper JA
Developmental cell27(4)399-411(2013 Nov)

F-box and WD repeat domain-containing-7 (Fbxw7) protein targets endoplasmic reticulum-anchored osteogenic and chondrogenic transcriptional factors for degradation.
Yumimoto K, Matsumoto M, Onoyama I, Imaizumi K, Nakayama KI
The Journal of biological chemistry288(40)28488-502(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Downregulation of Rbx1 caused accumulation of Emi1.
Title: The role of RING box protein 1 in mouse oocyte meiotic maturation.

a model by which N-terminal cleavage of RBX1 impairs its activity and promotes susceptibility to ER stress induction.
Title: Endoplasmic reticulum stress induces a caspase-dependent N-terminal cleavage of RBX1 protein in B cells.

Nrf2 regulates Cul3-Rbx1 by controlling regulation of expression and induction of Cul3-Rbx1
Title: An autoregulatory loop between Nrf2 and Cul3-Rbx1 controls their cellular abundance.

in vivo physiological function of RBX1 is to ensure cell proliferation by preventing p27 accumulation during the early stage of embryonic development
Title: RBX1/ROC1 disruption results in early embryonic lethality due to proliferation failure, partially rescued by simultaneous loss of p27.

SCCRO recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation
Title: SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation.

The following Rbx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rbx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22421	NM_019712.3 Latest version!	Mus musculus ring-box 1 (Rbx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rbx1 ( NM_019712.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062686.1 Mus musculus ring-box 1 (Rbx1), mRNA.

Price & Availability↑

CloneID	OMu22421
Clone ID Related Accession (Same CDS sequence)	NM_019712.3
Accession Version	NM_019712.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RBX1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ082632.1, BC056992.1, CB522825.1 and BB807275.1. On Aug 12, 2006 this sequence version replaced NM_019712.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK005127.1, BC027396.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGATGGATGT GGATACCCCC AGCGGCACCA ACAGCGGCGC GGGCAAGAAG 
CGCTTTGAAG TTAAAAAGTG GAATGCAGTG GCCCTCTGGG CCTGGGACAT TGTGGTTGAT 
AACTGTGCCA TCTGCAGGAA CCACATTATG GATCTTTGTA TCGAATGTCA GGCCAACCAG 
GCGTCAGCTA CTTCCGAAGA GTGTACGGTT GCATGGGGAG TCTGCAACCA TGCTTTTCAT 
TTCCACTGCA TCTCTCGATG GCTCAAAACG AGGCAGGTGT GTCCGTTGGA CAACAGAGAG 
TGGGAGTTCC AGAAGTATGG GCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062686.1
CDS	58..384
Translation	MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH

Target ORF information:

RefSeq Version	NM_019712.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring-box 1 (Rbx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019712.3

ORF Insert Sequence:

ATGGCGGCGG CGATGGATGT GGATACCCCC AGCGGCACCA ACAGCGGCGC GGGCAAGAAG 
CGCTTTGAAG TTAAAAAGTG GAATGCAGTG GCCCTCTGGG CCTGGGACAT TGTGGTTGAT 
AACTGTGCCA TCTGCAGGAA CCACATTATG GATCTTTGTA TCGAATGTCA GGCCAACCAG 
GCGTCAGCTA CTTCCGAAGA GTGTACGGTT GCATGGGGAG TCTGCAACCA TGCTTTTCAT 
TTCCACTGCA TCTCTCGATG GCTCAAAACG AGGCAGGTGT GTCCGTTGGA CAACAGAGAG 
TGGGAGTTCC AGAAGTATGG GCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CUL9 mediates the functions of the 3M complex and ubiquitylates survivin to maintain genome integrity.
Molecular cell54(5)805-19(2014 Jun)
Li Z,Pei XH,Yan J,Yan F,Cappell KM,Whitehurst AW,Xiong Y

CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.
Science (New York, N.Y.)342(6165)1518-21(2013 Dec)
Yu C,Zhang YL,Pan WW,Li XM,Wang ZW,Ge ZJ,Zhou JJ,Cang Y,Tong C,Sun QY,Fan HY

Rbx2 regulates neuronal migration through different cullin 5-RING ligase adaptors.
Developmental cell27(4)399-411(2013 Nov)
Sim? S,Cooper JA

F-box and WD repeat domain-containing-7 (Fbxw7) protein targets endoplasmic reticulum-anchored osteogenic and chondrogenic transcriptional factors for degradation.
The Journal of biological chemistry288(40)28488-502(2013 Oct)
Yumimoto K,Matsumoto M,Onoyama I,Imaizumi K,Nakayama KI