Diaph3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Diaph3
Entrez Gene ID	56419
Full Name	diaphanous related formin 3
Synonyms	4930417P13Rik,Dia2,Diap3,Drf3,mDia2,mKIAA4117,p134MDia2
General protein information	Preferred Names diaphanous related formin 3 Names protein diaphanous homolog 3 diaphanous homolog 3 diaphanous-related formin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 E1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006519312.3	XP_006519375.1	protein diaphanous homolog 3 isoform X2
NM_019670.1	NP_062644.1	protein diaphanous homolog 3
XM_011245123.2	XP_011243425.1	protein diaphanous homolog 3 isoform X1
XM_006519312.4	XP_006519375.1	protein diaphanous homolog 3 isoform X2
NM_019670.2	NP_062644.1	protein diaphanous homolog 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIAPH3	NP_001245295.1
Pan troglodytes (chimpanzee)	DIAPH3	XP_509808.3
Canis lupus familiaris (dog)	DIAPH3	XP_005633999.1
Rattus norvegicus (Norway rat)	Diaph3	XP_002728345.2
Xenopus tropicalis (tropical clawed frog)	diaph3	XP_002936971.2
	DIAPH3	XP_002800823.1
Bos taurus (cattle)	DIAPH3	NP_001179543.1
Mus musculus (house mouse)	Diap3	NP_062644.1

Gene Ontology
Bibliography

Related articles in PubMed

Histone deacetylase 6 regulates cytokinesis and erythrocyte enucleation through deacetylation of formin protein mDia2.
Li X, Mei Y, Yan B, Vitriol E, Huang S, Ji P, Qiu Y
Haematologica102(6)984-994(2017 Jun)

Quantitative Proteomics Illuminates a Functional Interaction between mDia2 and the Proteasome.
Isogai T, van der Kammen R, Bleijerveld OB, Goerdayal SS, Argenzio E, Altelaar AF, Innocenti M
Journal of proteome research15(12)4624-4637(2016 Dec)

Lack of Diaph3 relaxes the spindle checkpoint causing the loss of neural progenitors.
Damiani D, Goffinet AM, Alberts A, Tissir F
Nature communications713509(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings add more complexity to the mDia2 activity cycle by showing that the open conformation may control actin dynamics also through actin-independent regulation of the proteasome
Title: Quantitative Proteomics Illuminates a Functional Interaction between mDia2 and the Proteasome.

Ineffective erythropoiesis caused by binucleated late-stage erythroblasts in mDia2 hematopoietic specific knockout mice.
Title: Ineffective erythropoiesis caused by binucleated late-stage erythroblasts in mDia2 hematopoietic specific knockout mice.

the interactome of auto-inhibited and constitutively active mDia2, is reported.
Title: Proteomic analyses uncover a new function and mode of action for mouse homolog of Diaphanous 2 (mDia2).

mDia2-deficient erythroid cells differentiate normally, though in a delayed manner, but exhibit cytokinesis failure with decreased accumulation of F-actin in the cleavage furrow during late differentiation from proerythroblasts
Title: Loss of a Rho-regulated actin nucleator, mDia2, impairs cytokinesis during mouse fetal erythropoiesis.

Diaphanous homolog 3 (Diap3) overexpression causes progressive hearing loss and inner hair cell defects in a transgenic mouse model of human deafness.
Title: Diaphanous homolog 3 (Diap3) overexpression causes progressive hearing loss and inner hair cell defects in a transgenic mouse model of human deafness.

The following Diaph3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Diaph3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu50192	XM_006519312.4 Latest version!	Mus musculus diaphanous related formin 3 (Diaph3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu17668	NM_019670.2 Latest version!	Mus musculus diaphanous related formin 3 (Diaph3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu69653	XM_011245123.2 Latest version!	Mus musculus diaphanous related formin 3 (Diaph3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu50192	XM_006519312.3	Mus musculus diaphanous related formin 3 (Diaph3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu17668	NM_019670.1	Mus musculus diaphanous related formin 3 (Diaph3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Diaph3 ( XM_006519312.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519375.1 Mus musculus diaphanous related formin 3 (Diaph3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu50192
Clone ID Related Accession (Same CDS sequence)	XM_006519312.3 , XM_006519312.4
Accession Version	XM_006519312.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006519312.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGC ACCGGGCGCG CGCTCTCGGC CGGGACAGCA AGTCGTCGCG GAGGAAGGGC 
TTGCAGTCCG CGCCGCCCGC TGGCCCCTAC GAGCCCGGGG AGAAGCGACC CAAGTTGCAT 
TTAAATATTA GAACACTGAC AGATGATATG CTGGACAAAT TTGCCAGTAT AAGAATTCCA 
GGGAGCAAGA AAGAGAGACC TCCCCTTCCC CACCTGAAGA CTGTGTCTGG GATCAGTGAC 
AGCTCATCAC TGTCCTCAGA GACAATGGAA AACAACCCAA AGGCGCTGCC AGAGAGTGAA 
GTCTTGAAGC TTTTTGAGAA GATGATGGAA GATATGAATT TAAATGAAGA TAAAAAGGCA 
CCATTGCGGG AAAAAGACTT CGGTATCAAA AAAGAAATGG TGATGCAGTA CATTAATACT 
GCTTCTAAGA CAGGAAGTCT TAGAAGTAGC CGACAGATCT CACCTCAGGA ATTTCTCCAT 
GAGCTGAAAA TGGGTTACAC AGACGAGAGA CTTTTCACGT ATCTGGAGTC ACTCCGAGTA 
TCATTGACCA GTCATCCTGT GAGTTGGGTG CAAAGCTTTG GACACGAGGG ACTCGGATTA 
TTGCTGGACA TTTTGGAAAA ACTAATTAAT GGGCAAATCC AAGAAAAAGT TGTGAAGAAG 
ACTCAGCACA AAGTCATCCA GTGTCTGAGA GCCCTGATGA ACACACAGTA TGGCTTAGAA 
AGGATTATGA GTGACAAGAG GAGTCTTTCC TTGTTGGCAA AAGCCATGGA TCCCAGGCAG 
CCCGCTATGA TGGCAGACGT GGTGAAGCTT CTGTCTGCAG TGTGCATTGT CGGCGAGGAA 
AGCATCCTTG AAGAAGTGTT AGAAGCCTTG ACTTCAGCTG GAGAAGAAAG AAAGATTGAC 
AGATTTTTTT CCATTGTGGA AGGCCTCCGG CATAACTCAG TGAACCTGCA AGTTGCTTGT 
ATGCAGCTCA TAAATGCTCT CGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACCTC 
AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATATTGC CAAACTTAAA GGGCATTAAG 
AATGATGGCC TGGATATACA ACTTAAAGTC TTTGATGAGC ACAAAGAAGA AGATTTGAGT 
GAGTTTTTCC ATCGCCTTGA AGACATTAGA GCTGAACTTG ATGAAGCATC TGATGTTTAC 
AGCATGTTAT GGGACACAGT TAAGGAAACT CGAGCAGAGG GACATTTTCT TTCTATTCTT 
CAGCATCTCC TGCTCATTCG CAATGATCGT TTTATAAGAG AGCAGTATTT CAAATTAATT 
GATGAGTGTG TGTCACAGAT TGTATTACAT AGAGATGGAA CGGACCCTGA CTTCACATAC 
AGAAAAAGAC TAGATTTGGA CTTAAGTCAG TTTGTAGATG TTTGCATAGA TCAGGCCAAA 
CTAGATGAGT GGGAAGAGAA AGCATCCGAA CATTGCAAGA AATTTGAAAA AGAGTGTACT 
GACCACCAAG AAACCCAGGC TCAATTGCAG AAAAGAGAGG CAAAGATTAA TGAGCTTCAA 
GCAGAGTTAC AAGCTTTTAA ATCCCAGTTT GGTGCCTTGC CACCTGGTAC AAAAATTCCT 
TTGCAACCTT CAGTAGAAGG TGAAGCTGGC CCTTCAGCCC TTCCTCCTGC CCCACCAGCA 
CTCAGTGGAG GAGTGCCGCC TCCCCCACCG CCCCCTCCTC CACCACCCCC ACCACTCCCA 
GGAATGCCAA TGCCATTTGG TGGCCCTGTA CCACCACCAC CTCCTCTGGG ATTCCTGGGT 
GGGCAAAGCT CTATTCCATT AAACCTGCCA TTTGGTTTGA AACCAAAGAA AGAATTTAAG 
CCTGAAATCA GCATGAGAAG ATTGAATTGG TTAAAGATCG GACCAAATGA AATGTCTGAG 
AACTGCTTCT GGATCAAAGT AAATGAAAAT AAGTATGAAA ATAGGGATTT GCTTTGTAAA 
CTTGAGAACA CTTTTTGTTG CCAAGAAAAA GAGAAAAGGA ATACAAATGA CTTTGATGAG 
AAGAAAGTTA TTAAGAAGAG AATGAAGGAA CTTAAATTTC TAGATCCTAA AATTGCTCAG 
AACCTTTCAA TCTTCCTGAG CTCCTTCCGG GTGCCATATG AGAAAATCAG GACGATGATA 
TTGGAAGTGG ATGAAACACA GTTGTCAGAG TCCATGATTC AGAACTTAAT AAAGCACCTT 
CCTGATGAGG AGCAGTTGAA GTCATTGTCC CAGTTTAGAA GTGACTATAA CAGTTTGTGT 
GAGCCTGAGC AGTTCGCTGT TGTGATGAGC AATGTGAAGA GACTCCGGCC ACGGCTCAGT 
GCTATTCTCT TTAAGCTTCA ATTTGAAGAG CAGGTGAACA ACATCAAACC TGACATCATG 
GCTGTCAGTA CTGCCTGCGA GGAGATCAAG AAGAGCAAAG GCTTTAGCAA GTTGCTGGAA 
CTTGTGTTGC TAATGGGAAA CTACATGAAT GCTGGCTCCC GGAATGCTCA AACCTTCGGA 
TTTGACCTTA GCTCTCTCTG TAAACTGAAG GATACAAAAT CTGCAGATCA GAAAACCACA 
CTCCTCCATT TCCTGGTAGA TGTATGTGAA GAAAAGCATG CTGACATCCT TCACTTTGTG 
GACGATTTGG CACATTTAGA CAAAGCTAGC AGAGTCTCTG TAGAAATGCT GGAAAAGAAC 
GTGAAGCAGA TGGGAAGGCA GCTTCAACAG CTTGAGAAGA ATTTGGAAAC CTTTCCCCCT 
CCTGAGGACT TGCATGACAA GTTTGTGATA AAGATGTCCA GCTTCGTTAT CAGTGCGAAC 
GAGCAGTATG AAAAACTCTC CACACTACTG GGCAGCATGA CACAATTGTA CCAGAGTATA 
ATGGGCTACT ATGCTGTCGA CATGAAGAAG GTTTCCGTGG AAGAGTTTTT TAATGATCTG 
AACAACTTCA GAACTTCATT TATGCTAGCA TTAAAGGAAA ACATCAAAAA ACGAGAAGCA 
GCAGAAAAGG AGAAACGTGC CAGGATAGCG AAAGAGCGAG CAGAGAAAGA GCGACTTGAA 
CGCCAGCAAG AGAAAAAGCG CTTACTAGAA ATGAAAACTG TCCACAGGAT GCTAACAGAA 
CTTGATCCGA GGAGACATGG CATTTGCAGT GTTCCATCAA GTGTGCACCC CACCATCGCA 
GGACACGTGC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519375.1
CDS	134..3328
Translation	MERHRARALGRDSKSSRRKGLQSAPPAGPYEPGEKRPKLHLNIRTLTDDMLDKFASIRIPGSKKERPPLPHLKTVSGISDSSSLSSETMENNPKALPESEVLKLFEKMMEDMNLNEDKKAPLREKDFGIKKEMVMQYINTASKTGSLRSSRQISPQEFLHELKMGYTDERLFTYLESLRVSLTSHPVSWVQSFGHEGLGLLLDILEKLINGQIQEKVVKKTQHKVIQCLRALMNTQYGLERIMSDKRSLSLLAKAMDPRQPAMMADVVKLLSAVCIVGEESILEEVLEALTSAGEERKIDRFFSIVEGLRHNSVNLQVACMQLINALVTSPDDLDFRLHLRNEFMRCGLKEILPNLKGIKNDGLDIQLKVFDEHKEEDLSEFFHRLEDIRAELDEASDVYSMLWDTVKETRAEGHFLSILQHLLLIRNDRFIREQYFKLIDECVSQIVLHRDGTDPDFTYRKRLDLDLSQFVDVCIDQAKLDEWEEKASEHCKKFEKECTDHQETQAQLQKREAKINELQAELQAFKSQFGALPPGTKIPLQPSVEGEAGPSALPPAPPALSGGVPPPPPPPPPPPPPLPGMPMPFGGPVPPPPPLGFLGGQSSIPLNLPFGLKPKKEFKPEISMRRLNWLKIGPNEMSENCFWIKVNENKYENRDLLCKLENTFCCQEKEKRNTNDFDEKKVIKKRMKELKFLDPKIAQNLSIFLSSFRVPYEKIRTMILEVDETQLSESMIQNLIKHLPDEEQLKSLSQFRSDYNSLCEPEQFAVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKGFSKLLELVLLMGNYMNAGSRNAQTFGFDLSSLCKLKDTKSADQKTTLLHFLVDVCEEKHADILHFVDDLAHLDKASRVSVEMLEKNVKQMGRQLQQLEKNLETFPPPEDLHDKFVIKMSSFVISANEQYEKLSTLLGSMTQLYQSIMGYYAVDMKKVSVEEFFNDLNNFRTSFMLALKENIKKREAAEKEKRARIAKERAEKERLERQQEKKRLLEMKTVHRMLTELDPRRHGICSVPSSVHPTIAGHVP

Target ORF information:

RefSeq Version	XM_006519312.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 3 (Diaph3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519312.4

ORF Insert Sequence:

ATGGAGAGGC ACCGGGCGCG CGCTCTCGGC CGGGACAGCA AGTCGTCGCG GAGGAAGGGC 
TTGCAGTCCG CGCCGCCCGC TGGCCCCTAC GAGCCCGGGG AGAAGCGACC CAAGTTGCAT 
TTAAATATTA GAACACTGAC AGATGATATG CTGGACAAAT TTGCCAGTAT AAGAATTCCA 
GGGAGCAAGA AAGAGAGACC TCCCCTTCCC CACCTGAAGA CTGTGTCTGG GATCAGTGAC 
AGCTCATCAC TGTCCTCAGA GACAATGGAA AACAACCCAA AGGCGCTGCC AGAGAGTGAA 
GTCTTGAAGC TTTTTGAGAA GATGATGGAA GATATGAATT TAAATGAAGA TAAAAAGGCA 
CCATTGCGGG AAAAAGACTT CGGTATCAAA AAAGAAATGG TGATGCAGTA CATTAATACT 
GCTTCTAAGA CAGGAAGTCT TAGAAGTAGC CGACAGATCT CACCTCAGGA ATTTCTCCAT 
GAGCTGAAAA TGGGTTACAC AGACGAGAGA CTTTTCACGT ATCTGGAGTC ACTCCGAGTA 
TCATTGACCA GTCATCCTGT GAGTTGGGTG CAAAGCTTTG GACACGAGGG ACTCGGATTA 
TTGCTGGACA TTTTGGAAAA ACTAATTAAT GGGCAAATCC AAGAAAAAGT TGTGAAGAAG 
ACTCAGCACA AAGTCATCCA GTGTCTGAGA GCCCTGATGA ACACACAGTA TGGCTTAGAA 
AGGATTATGA GTGACAAGAG GAGTCTTTCC TTGTTGGCAA AAGCCATGGA TCCCAGGCAG 
CCCGCTATGA TGGCAGACGT GGTGAAGCTT CTGTCTGCAG TGTGCATTGT CGGCGAGGAA 
AGCATCCTTG AAGAAGTGTT AGAAGCCTTG ACTTCAGCTG GAGAAGAAAG AAAGATTGAC 
AGATTTTTTT CCATTGTGGA AGGCCTCCGG CATAACTCAG TGAACCTGCA AGTTGCTTGT 
ATGCAGCTCA TAAATGCTCT CGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACCTC 
AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATATTGC CAAACTTAAA GGGCATTAAG 
AATGATGGCC TGGATATACA ACTTAAAGTC TTTGATGAGC ACAAAGAAGA AGATTTGAGT 
GAGTTTTTCC ATCGCCTTGA AGACATTAGA GCTGAACTTG ATGAAGCATC TGATGTTTAC 
AGCATGTTAT GGGACACAGT TAAGGAAACT CGAGCAGAGG GACATTTTCT TTCTATTCTT 
CAGCATCTCC TGCTCATTCG CAATGATCGT TTTATAAGAG AGCAGTATTT CAAATTAATT 
GATGAGTGTG TGTCACAGAT TGTATTACAT AGAGATGGAA CGGACCCTGA CTTCACATAC 
AGAAAAAGAC TAGATTTGGA CTTAAGTCAG TTTGTAGATG TTTGCATAGA TCAGGCCAAA 
CTAGATGAGT GGGAAGAGAA AGCATCCGAA CATTGCAAGA AATTTGAAAA AGAGTGTACT 
GACCACCAAG AAACCCAGGC TCAATTGCAG AAAAGAGAGG CAAAGATTAA TGAGCTTCAA 
GCAGAGTTAC AAGCTTTTAA ATCCCAGTTT GGTGCCTTGC CACCTGGTAC AAAAATTCCT 
TTGCAACCTT CAGTAGAAGG TGAAGCTGGC CCTTCAGCCC TTCCTCCTGC CCCACCAGCA 
CTCAGTGGAG GAGTGCCGCC TCCCCCACCG CCCCCTCCTC CACCACCCCC ACCACTCCCA 
GGAATGCCAA TGCCATTTGG TGGCCCTGTA CCACCACCAC CTCCTCTGGG ATTCCTGGGT 
GGGCAAAGCT CTATTCCATT AAACCTGCCA TTTGGTTTGA AACCAAAGAA AGAATTTAAG 
CCTGAAATCA GCATGAGAAG ATTGAATTGG TTAAAGATCG GACCAAATGA AATGTCTGAG 
AACTGCTTCT GGATCAAAGT AAATGAAAAT AAGTATGAAA ATAGGGATTT GCTTTGTAAA 
CTTGAGAACA CTTTTTGTTG CCAAGAAAAA GAGAAAAGGA ATACAAATGA CTTTGATGAG 
AAGAAAGTTA TTAAGAAGAG AATGAAGGAA CTTAAATTTC TAGATCCTAA AATTGCTCAG 
AACCTTTCAA TCTTCCTGAG CTCCTTCCGG GTGCCATATG AGAAAATCAG GACGATGATA 
TTGGAAGTGG ATGAAACACA GTTGTCAGAG TCCATGATTC AGAACTTAAT AAAGCACCTT 
CCTGATGAGG AGCAGTTGAA GTCATTGTCC CAGTTTAGAA GTGACTATAA CAGTTTGTGT 
GAGCCTGAGC AGTTCGCTGT TGTGATGAGC AATGTGAAGA GACTCCGGCC ACGGCTCAGT 
GCTATTCTCT TTAAGCTTCA ATTTGAAGAG CAGGTGAACA ACATCAAACC TGACATCATG 
GCTGTCAGTA CTGCCTGCGA GGAGATCAAG AAGAGCAAAG GCTTTAGCAA GTTGCTGGAA 
CTTGTGTTGC TAATGGGAAA CTACATGAAT GCTGGCTCCC GGAATGCTCA AACCTTCGGA 
TTTGACCTTA GCTCTCTCTG TAAACTGAAG GATACAAAAT CTGCAGATCA GAAAACCACA 
CTCCTCCATT TCCTGGTAGA TGTATGTGAA GAAAAGCATG CTGACATCCT TCACTTTGTG 
GACGATTTGG CACATTTAGA CAAAGCTAGC AGAGTCTCTG TAGAAATGCT GGAAAAGAAC 
GTGAAGCAGA TGGGAAGGCA GCTTCAACAG CTTGAGAAGA ATTTGGAAAC CTTTCCCCCT 
CCTGAGGACT TGCATGACAA GTTTGTGATA AAGATGTCCA GCTTCGTTAT CAGTGCGAAC 
GAGCAGTATG AAAAACTCTC CACACTACTG GGCAGCATGA CACAATTGTA CCAGAGTATA 
ATGGGCTACT ATGCTGTCGA CATGAAGAAG GTTTCCGTGG AAGAGTTTTT TAATGATCTG 
AACAACTTCA GAACTTCATT TATGCTAGCA TTAAAGGAAA ACATCAAAAA ACGAGAAGCA 
GCAGAAAAGG AGAAACGTGC CAGGATAGCG AAAGAGCGAG CAGAGAAAGA GCGACTTGAA 
CGCCAGCAAG AGAAAAAGCG CTTACTAGAA ATGAAAACTG TCCACAGGAT GCTAACAGAA 
CTTGATCCGA GGAGACATGG CATTTGCAGT GTTCCATCAA GTGTGCACCC CACCATCGCA 
GGACACGTGC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diaph3 ( NM_019670.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062644.1 Mus musculus diaphanous related formin 3 (Diaph3), mRNA.

Price & Availability↑

CloneID	OMu17668
Clone ID Related Accession (Same CDS sequence)	NM_019670.1 , NM_019670.2
Accession Version	NM_019670.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-11
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF094519.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF094519.1, BC085191.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAGGC ACCGGGCGCG CGCTCTCGGC CGGGACAGCA AGTCGTCGCG GAGGAAGGGC 
TTGCAGTCCG CGCCGCCCGC TGGCCCCTAC GAGCCCGGGG AGAAGCGACC CAAGTTGCAT 
TTAAATATTA GAACACTGAC AGATGATATG CTGGACAAAT TTGCCAGTAT AAGAATTCCA 
GGGAGCAAGA AAGAGAGACC TCCCCTTCCC CACCTGAAGA CTGTGTCTGG GATCAGTGAC 
AGCTCATCAC TGTCCTCAGA GACAATGGAA AACAACCCAA AGGCGCTGCC AGAGAGTGAA 
GTCTTGAAGC TTTTTGAGAA GATGATGGAA GATATGAATT TAAATGAAGA TAAAAAGGCA 
CCATTGCGGG AAAAAGACTT CGGTATCAAA AAAGAAATGG TGATGCAGTA CATTAATACT 
GCTTCTAAGA CAGGAAGTCT TAGAAGTAGC CGACAGATCT CACCTCAGGA ATTTCTCCAT 
GAGCTGAAAA TGGGTTACAC AGACGAGAGA CTTTTCACGT ATCTGGAGTC ACTCCGAGTA 
TCATTGACCA GTCATCCTGT GAGTTGGGTG CAAAGCTTTG GACACGAGGG ACTCGGATTA 
TTGCTGGACA TTTTGGAAAA ACTAATTAAT GGGCAAATCC AAGAAAAAGT TGTGAAGAAG 
ACTCAGCACA AAGTCATCCA GTGTCTGAGA GCCCTGATGA ACACACAGTA TGGCTTAGAA 
AGGATTATGA GTGACAAGAG GAGTCTTTCC TTGTTGGCAA AAGCCATGGA TCCCAGGCAG 
CCCGCTATGA TGGCAGACGT GGTGAAGCTT CTGTCTGCAG TGTGCATTGT CGGCGAGGAA 
AGCATCCTTG AAGAAGTGTT AGAAGCCTTG ACTTCAGCTG GAGAAGAAAG AAAGATTGAC 
AGATTTTTTT CCATTGTGGA AGGCCTCCGG CATAACTCAG TGAACCTGCA AGTTGCTTGT 
ATGCAGCTCA TAAATGCTCT CGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACCTC 
AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATATTGC CAAACTTAAA GGGCATTAAG 
AATGATGGCC TGGATATACA ACTTAAAGTC TTTGATGAGC ACAAAGAAGA AGATTTGAGT 
GAGTTTTTCC ATCGCCTTGA AGACATTAGA GCTGAACTTG ATGAAGCATC TGATGTTTAC 
AGCATGTTAT GGGACACAGT TAAGGAAACT CGAGCAGAGG GACATTTTCT TTCTATTCTT 
CAGCATCTCC TGCTCATTCG CAATGATCGT TTTATAAGAG AGCAGTATTT CAAATTAATT 
GATGAGTGTG TGTCACAGAT TGTATTACAT AGAGATGGAA CGGACCCTGA CTTCACATAC 
AGAAAAAGAC TAGATTTGGA CTTAAGTCAG TTTGTAGATG TTTGCATAGA TCAGGCCAAA 
CTAGATGAGT GGGAAGAGAA AGCATCCGAA CATTGCAAGA AATTTGAAAA AGAGTGTACT 
GACCACCAAG AAACCCAGGC TCAATTGCAG AAAAGAGAGG CAAAGATTAA TGAGCTTCAA 
GCAGAGTTAC AAGCTTTTAA ATCCCAGTTT GGTGCCTTGC CACCTGGTAC AAAAATTCCT 
TTGCAACCTT CAGTAGAAGG TGAAGCTGGC CCTTCAGCCC TTCCTCCTGC CCCACCAGCA 
CTCAGTGGAG GAGTGCCGCC TCCCCCACCG CCCCCTCCTC CACCACCCCC ACCACTCCCA 
GGAATGCCAA TGCCATTTGG TGGCCCTGTA CCACCACCAC CTCCTCTGGG ATTCCTGGGT 
GGGCAAAGCT CTATTCCATT AAACCTGCCA TTTGGTTTGA AACCAAAGAA AGAATTTAAG 
CCTGAAATCA GCATGAGAAG ATTGAATTGG TTAAAGATCG GACCAAATGA AATGTCTGAG 
AACTGCTTCT GGATCAAAGT AAATGAAAAT AAGTATGAAA ATAGGGATTT GCTTTGTAAA 
CTTGAGAACA CTTTTTGTTG CCAAGAAAAA GAGAAAAGGA ATACAAATGA CTTTGATGAG 
AAGAAAGTTA TTAAGAAGAG AATGAAGGAA CTTAAATTTC TAGATCCTAA AATTGCTCAG 
AACCTTTCAA TCTTCCTGAG CTCCTTCCGG GTGCCATATG AGAAAATCAG GACGATGATA 
TTGGAAGTGG ATGAAACACA GTTGTCAGAG TCCATGATTC AGAACTTAAT AAAGCACCTT 
CCTGATGAGG AGCAGTTGAA GTCATTGTCC CAGTTTAGAA GTGACTATAA CAGTTTGTGT 
GAGCCTGAGC AGTTCGCTGT TGTGATGAGC AATGTGAAGA GACTCCGGCC ACGGCTCAGT 
GCTATTCTCT TTAAGCTTCA ATTTGAAGAG CAGGTGAACA ACATCAAACC TGACATCATG 
GCTGTCAGTA CTGCCTGCGA GGAGATCAAG AAGAGCAAAG GCTTTAGCAA GTTGCTGGAA 
CTTGTGTTGC TAATGGGAAA CTACATGAAT GCTGGCTCCC GGAATGCTCA AACCTTCGGA 
TTTGACCTTA GCTCTCTCTG TAAACTGAAG GATACAAAAT CTGCAGATCA GAAAACCACA 
CTCCTCCATT TCCTGGTAGA TGTATGTGAA GAAAAGCATG CTGACATCCT TCACTTTGTG 
GACGATTTGG CACATTTAGA CAAAGCTAGC AGAGTCTCTG TAGAAATGCT GGAAAAGAAC 
GTGAAGCAGA TGGGAAGGCA GCTTCAACAG CTTGAGAAGA ATTTGGAAAC CTTTCCCCCT 
CCTGAGGACT TGCATGACAA GTTTGTGATA AAGATGTCCA GCTTCGTTAT CAGTGCGAAC 
GAGCAGTATG AAAAACTCTC CACACTACTG GGCAGCATGA CACAATTGTA CCAGAGTATA 
ATGGGCTACT ATGCTGTCGA CATGAAGAAG GTTTCCGTGG AAGAGTTTTT TAATGATCTG 
AACAACTTCA GAACTTCATT TATGCTAGCA TTAAAGGAAA ACATCAAAAA ACGAGAAGCA 
GCAGAAAAGG AGAAACGTGC CAGGATAGCG AAAGAGCGAG CAGAGAAAGA GCGACTTGAA 
CGCCAGCAAG AGAAAAAGCG CTTACTAGAA ATGAAAACTG AGGGAGATGA GACAGGAGTG 
ATGGATAGTC TGCTGGAGGC CTTGCAGTCA GGGGCTGCCT TCCGCGACAG AAGAAAAAGG 
ACACCAAAGC TGAAAGATAT TCGGCAGAGT CTCAGCCCGA TGTCTCAGAG GCCTGTTCTC 
AAAGTTTGTA ACCATGAAAA TCAGAAAATG CAGTTGACAG AAGGGTCACG TCCACACCAC 
AGTATCAATT GCAACTCCAC CAGGACTCCA GTCGCCAAGG AGCTTAATTA TAATCTAGAC 
ACTCATGCGT CTACAGGGAG GATCAAGGCA GTTGAGAAGG AAGCCTGTAA TGCAGAAAGC 
AACAAAAAAA AGGAAATGGA ACTTCTTGGC TCTGTTGCTA AAAGCGAATC AGTTCCTGAA 
GTTGAAGCCC TGCTGGCAAG ATTACGAGCT TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062644.1
CDS	1..3516
Translation	MERHRARALGRDSKSSRRKGLQSAPPAGPYEPGEKRPKLHLNIRTLTDDMLDKFASIRIPGSKKERPPLPHLKTVSGISDSSSLSSETMENNPKALPESEVLKLFEKMMEDMNLNEDKKAPLREKDFGIKKEMVMQYINTASKTGSLRSSRQISPQEFLHELKMGYTDERLFTYLESLRVSLTSHPVSWVQSFGHEGLGLLLDILEKLINGQIQEKVVKKTQHKVIQCLRALMNTQYGLERIMSDKRSLSLLAKAMDPRQPAMMADVVKLLSAVCIVGEESILEEVLEALTSAGEERKIDRFFSIVEGLRHNSVNLQVACMQLINALVTSPDDLDFRLHLRNEFMRCGLKEILPNLKGIKNDGLDIQLKVFDEHKEEDLSEFFHRLEDIRAELDEASDVYSMLWDTVKETRAEGHFLSILQHLLLIRNDRFIREQYFKLIDECVSQIVLHRDGTDPDFTYRKRLDLDLSQFVDVCIDQAKLDEWEEKASEHCKKFEKECTDHQETQAQLQKREAKINELQAELQAFKSQFGALPPGTKIPLQPSVEGEAGPSALPPAPPALSGGVPPPPPPPPPPPPPLPGMPMPFGGPVPPPPPLGFLGGQSSIPLNLPFGLKPKKEFKPEISMRRLNWLKIGPNEMSENCFWIKVNENKYENRDLLCKLENTFCCQEKEKRNTNDFDEKKVIKKRMKELKFLDPKIAQNLSIFLSSFRVPYEKIRTMILEVDETQLSESMIQNLIKHLPDEEQLKSLSQFRSDYNSLCEPEQFAVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKGFSKLLELVLLMGNYMNAGSRNAQTFGFDLSSLCKLKDTKSADQKTTLLHFLVDVCEEKHADILHFVDDLAHLDKASRVSVEMLEKNVKQMGRQLQQLEKNLETFPPPEDLHDKFVIKMSSFVISANEQYEKLSTLLGSMTQLYQSIMGYYAVDMKKVSVEEFFNDLNNFRTSFMLALKENIKKREAAEKEKRARIAKERAEKERLERQQEKKRLLEMKTEGDETGVMDSLLEALQSGAAFRDRRKRTPKLKDIRQSLSPMSQRPVLKVCNHENQKMQLTEGSRPHHSINCNSTRTPVAKELNYNLDTHASTGRIKAVEKEACNAESNKKKEMELLGSVAKSESVPEVEALLARLRAL

Target ORF information:

RefSeq Version	NM_019670.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 3 (Diaph3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019670.1

ORF Insert Sequence:

ATGGAGAGGC ACCGGGCGCG CGCTCTCGGC CGGGACAGCA AGTCGTCGCG GAGGAAGGGC 
TTGCAGTCCG CGCCGCCCGC TGGCCCCTAC GAGCCCGGGG AGAAGCGACC CAAGTTGCAT 
TTAAATATTA GAACACTGAC AGATGATATG CTGGACAAAT TTGCCAGTAT AAGAATTCCA 
GGGAGCAAGA AAGAGAGACC TCCCCTTCCC CACCTGAAGA CTGTGTCTGG GATCAGTGAC 
AGCTCATCAC TGTCCTCAGA GACAATGGAA AACAACCCAA AGGCGCTGCC AGAGAGTGAA 
GTCTTGAAGC TTTTTGAGAA GATGATGGAA GATATGAATT TAAATGAAGA TAAAAAGGCA 
CCATTGCGGG AAAAAGACTT CGGTATCAAA AAAGAAATGG TGATGCAGTA CATTAATACT 
GCTTCTAAGA CAGGAAGTCT TAGAAGTAGC CGACAGATCT CACCTCAGGA ATTTCTCCAT 
GAGCTGAAAA TGGGTTACAC AGACGAGAGA CTTTTCACGT ATCTGGAGTC ACTCCGAGTA 
TCATTGACCA GTCATCCTGT GAGTTGGGTG CAAAGCTTTG GACACGAGGG ACTCGGATTA 
TTGCTGGACA TTTTGGAAAA ACTAATTAAT GGGCAAATCC AAGAAAAAGT TGTGAAGAAG 
ACTCAGCACA AAGTCATCCA GTGTCTGAGA GCCCTGATGA ACACACAGTA TGGCTTAGAA 
AGGATTATGA GTGACAAGAG GAGTCTTTCC TTGTTGGCAA AAGCCATGGA TCCCAGGCAG 
CCCGCTATGA TGGCAGACGT GGTGAAGCTT CTGTCTGCAG TGTGCATTGT CGGCGAGGAA 
AGCATCCTTG AAGAAGTGTT AGAAGCCTTG ACTTCAGCTG GAGAAGAAAG AAAGATTGAC 
AGATTTTTTT CCATTGTGGA AGGCCTCCGG CATAACTCAG TGAACCTGCA AGTTGCTTGT 
ATGCAGCTCA TAAATGCTCT CGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACCTC 
AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATATTGC CAAACTTAAA GGGCATTAAG 
AATGATGGCC TGGATATACA ACTTAAAGTC TTTGATGAGC ACAAAGAAGA AGATTTGAGT 
GAGTTTTTCC ATCGCCTTGA AGACATTAGA GCTGAACTTG ATGAAGCATC TGATGTTTAC 
AGCATGTTAT GGGACACAGT TAAGGAAACT CGAGCAGAGG GACATTTTCT TTCTATTCTT 
CAGCATCTCC TGCTCATTCG CAATGATCGT TTTATAAGAG AGCAGTATTT CAAATTAATT 
GATGAGTGTG TGTCACAGAT TGTATTACAT AGAGATGGAA CGGACCCTGA CTTCACATAC 
AGAAAAAGAC TAGATTTGGA CTTAAGTCAG TTTGTAGATG TTTGCATAGA TCAGGCCAAA 
CTAGATGAGT GGGAAGAGAA AGCATCCGAA CATTGCAAGA AATTTGAAAA AGAGTGTACT 
GACCACCAAG AAACCCAGGC TCAATTGCAG AAAAGAGAGG CAAAGATTAA TGAGCTTCAA 
GCAGAGTTAC AAGCTTTTAA ATCCCAGTTT GGTGCCTTGC CACCTGGTAC AAAAATTCCT 
TTGCAACCTT CAGTAGAAGG TGAAGCTGGC CCTTCAGCCC TTCCTCCTGC CCCACCAGCA 
CTCAGTGGAG GAGTGCCGCC TCCCCCACCG CCCCCTCCTC CACCACCCCC ACCACTCCCA 
GGAATGCCAA TGCCATTTGG TGGCCCTGTA CCACCACCAC CTCCTCTGGG ATTCCTGGGT 
GGGCAAAGCT CTATTCCATT AAACCTGCCA TTTGGTTTGA AACCAAAGAA AGAATTTAAG 
CCTGAAATCA GCATGAGAAG ATTGAATTGG TTAAAGATCG GACCAAATGA AATGTCTGAG 
AACTGCTTCT GGATCAAAGT AAATGAAAAT AAGTATGAAA ATAGGGATTT GCTTTGTAAA 
CTTGAGAACA CTTTTTGTTG CCAAGAAAAA GAGAAAAGGA ATACAAATGA CTTTGATGAG 
AAGAAAGTTA TTAAGAAGAG AATGAAGGAA CTTAAATTTC TAGATCCTAA AATTGCTCAG 
AACCTTTCAA TCTTCCTGAG CTCCTTCCGG GTGCCATATG AGAAAATCAG GACGATGATA 
TTGGAAGTGG ATGAAACACA GTTGTCAGAG TCCATGATTC AGAACTTAAT AAAGCACCTT 
CCTGATGAGG AGCAGTTGAA GTCATTGTCC CAGTTTAGAA GTGACTATAA CAGTTTGTGT 
GAGCCTGAGC AGTTCGCTGT TGTGATGAGC AATGTGAAGA GACTCCGGCC ACGGCTCAGT 
GCTATTCTCT TTAAGCTTCA ATTTGAAGAG CAGGTGAACA ACATCAAACC TGACATCATG 
GCTGTCAGTA CTGCCTGCGA GGAGATCAAG AAGAGCAAAG GCTTTAGCAA GTTGCTGGAA 
CTTGTGTTGC TAATGGGAAA CTACATGAAT GCTGGCTCCC GGAATGCTCA AACCTTCGGA 
TTTGACCTTA GCTCTCTCTG TAAACTGAAG GATACAAAAT CTGCAGATCA GAAAACCACA 
CTCCTCCATT TCCTGGTAGA TGTATGTGAA GAAAAGCATG CTGACATCCT TCACTTTGTG 
GACGATTTGG CACATTTAGA CAAAGCTAGC AGAGTCTCTG TAGAAATGCT GGAAAAGAAC 
GTGAAGCAGA TGGGAAGGCA GCTTCAACAG CTTGAGAAGA ATTTGGAAAC CTTTCCCCCT 
CCTGAGGACT TGCATGACAA GTTTGTGATA AAGATGTCCA GCTTCGTTAT CAGTGCGAAC 
GAGCAGTATG AAAAACTCTC CACACTACTG GGCAGCATGA CACAATTGTA CCAGAGTATA 
ATGGGCTACT ATGCTGTCGA CATGAAGAAG GTTTCCGTGG AAGAGTTTTT TAATGATCTG 
AACAACTTCA GAACTTCATT TATGCTAGCA TTAAAGGAAA ACATCAAAAA ACGAGAAGCA 
GCAGAAAAGG AGAAACGTGC CAGGATAGCG AAAGAGCGAG CAGAGAAAGA GCGACTTGAA 
CGCCAGCAAG AGAAAAAGCG CTTACTAGAA ATGAAAACTG AGGGAGATGA GACAGGAGTG 
ATGGATAGTC TGCTGGAGGC CTTGCAGTCA GGGGCTGCCT TCCGCGACAG AAGAAAAAGG 
ACACCAAAGC TGAAAGATAT TCGGCAGAGT CTCAGCCCGA TGTCTCAGAG GCCTGTTCTC 
AAAGTTTGTA ACCATGAAAA TCAGAAAATG CAGTTGACAG AAGGGTCACG TCCACACCAC 
AGTATCAATT GCAACTCCAC CAGGACTCCA GTCGCCAAGG AGCTTAATTA TAATCTAGAC 
ACTCATGCGT CTACAGGGAG GATCAAGGCA GTTGAGAAGG AAGCCTGTAA TGCAGAAAGC 
AACAAAAAAA AGGAAATGGA ACTTCTTGGC TCTGTTGCTA AAAGCGAATC AGTTCCTGAA 
GTTGAAGCCC TGCTGGCAAG ATTACGAGCT TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diaph3 ( NM_019670.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062644.1 Mus musculus diaphanous related formin 3 (Diaph3), mRNA.

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CloneID	OMu17668
Clone ID Related Accession (Same CDS sequence)	NM_019670.1 , NM_019670.2
Accession Version	NM_019670.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC085191.1, AF094519.1 and AC158988.3. On Apr 17, 2018 this sequence version replaced NM_019670.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085191.1, AK220493.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGGC ACCGGGCGCG CGCTCTCGGC CGGGACAGCA AGTCGTCGCG GAGGAAGGGC 
TTGCAGTCCG CGCCGCCCGC TGGCCCCTAC GAGCCCGGGG AGAAGCGACC CAAGTTGCAT 
TTAAATATTA GAACACTGAC AGATGATATG CTGGACAAAT TTGCCAGTAT AAGAATTCCA 
GGGAGCAAGA AAGAGAGACC TCCCCTTCCC CACCTGAAGA CTGTGTCTGG GATCAGTGAC 
AGCTCATCAC TGTCCTCAGA GACAATGGAA AACAACCCAA AGGCGCTGCC AGAGAGTGAA 
GTCTTGAAGC TTTTTGAGAA GATGATGGAA GATATGAATT TAAATGAAGA TAAAAAGGCA 
CCATTGCGGG AAAAAGACTT CGGTATCAAA AAAGAAATGG TGATGCAGTA CATTAATACT 
GCTTCTAAGA CAGGAAGTCT TAGAAGTAGC CGACAGATCT CACCTCAGGA ATTTCTCCAT 
GAGCTGAAAA TGGGTTACAC AGACGAGAGA CTTTTCACGT ATCTGGAGTC ACTCCGAGTA 
TCATTGACCA GTCATCCTGT GAGTTGGGTG CAAAGCTTTG GACACGAGGG ACTCGGATTA 
TTGCTGGACA TTTTGGAAAA ACTAATTAAT GGGCAAATCC AAGAAAAAGT TGTGAAGAAG 
ACTCAGCACA AAGTCATCCA GTGTCTGAGA GCCCTGATGA ACACACAGTA TGGCTTAGAA 
AGGATTATGA GTGACAAGAG GAGTCTTTCC TTGTTGGCAA AAGCCATGGA TCCCAGGCAG 
CCCGCTATGA TGGCAGACGT GGTGAAGCTT CTGTCTGCAG TGTGCATTGT CGGCGAGGAA 
AGCATCCTTG AAGAAGTGTT AGAAGCCTTG ACTTCAGCTG GAGAAGAAAG AAAGATTGAC 
AGATTTTTTT CCATTGTGGA AGGCCTCCGG CATAACTCAG TGAACCTGCA AGTTGCTTGT 
ATGCAGCTCA TAAATGCTCT CGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACCTC 
AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATATTGC CAAACTTAAA GGGCATTAAG 
AATGATGGCC TGGATATACA ACTTAAAGTC TTTGATGAGC ACAAAGAAGA AGATTTGAGT 
GAGTTTTTCC ATCGCCTTGA AGACATTAGA GCTGAACTTG ATGAAGCATC TGATGTTTAC 
AGCATGTTAT GGGACACAGT TAAGGAAACT CGAGCAGAGG GACATTTTCT TTCTATTCTT 
CAGCATCTCC TGCTCATTCG CAATGATCGT TTTATAAGAG AGCAGTATTT CAAATTAATT 
GATGAGTGTG TGTCACAGAT TGTATTACAT AGAGATGGAA CGGACCCTGA CTTCACATAC 
AGAAAAAGAC TAGATTTGGA CTTAAGTCAG TTTGTAGATG TTTGCATAGA TCAGGCCAAA 
CTAGATGAGT GGGAAGAGAA AGCATCCGAA CATTGCAAGA AATTTGAAAA AGAGTGTACT 
GACCACCAAG AAACCCAGGC TCAATTGCAG AAAAGAGAGG CAAAGATTAA TGAGCTTCAA 
GCAGAGTTAC AAGCTTTTAA ATCCCAGTTT GGTGCCTTGC CACCTGGTAC AAAAATTCCT 
TTGCAACCTT CAGTAGAAGG TGAAGCTGGC CCTTCAGCCC TTCCTCCTGC CCCACCAGCA 
CTCAGTGGAG GAGTGCCGCC TCCCCCACCG CCCCCTCCTC CACCACCCCC ACCACTCCCA 
GGAATGCCAA TGCCATTTGG TGGCCCTGTA CCACCACCAC CTCCTCTGGG ATTCCTGGGT 
GGGCAAAGCT CTATTCCATT AAACCTGCCA TTTGGTTTGA AACCAAAGAA AGAATTTAAG 
CCTGAAATCA GCATGAGAAG ATTGAATTGG TTAAAGATCG GACCAAATGA AATGTCTGAG 
AACTGCTTCT GGATCAAAGT AAATGAAAAT AAGTATGAAA ATAGGGATTT GCTTTGTAAA 
CTTGAGAACA CTTTTTGTTG CCAAGAAAAA GAGAAAAGGA ATACAAATGA CTTTGATGAG 
AAGAAAGTTA TTAAGAAGAG AATGAAGGAA CTTAAATTTC TAGATCCTAA AATTGCTCAG 
AACCTTTCAA TCTTCCTGAG CTCCTTCCGG GTGCCATATG AGAAAATCAG GACGATGATA 
TTGGAAGTGG ATGAAACACA GTTGTCAGAG TCCATGATTC AGAACTTAAT AAAGCACCTT 
CCTGATGAGG AGCAGTTGAA GTCATTGTCC CAGTTTAGAA GTGACTATAA CAGTTTGTGT 
GAGCCTGAGC AGTTCGCTGT TGTGATGAGC AATGTGAAGA GACTCCGGCC ACGGCTCAGT 
GCTATTCTCT TTAAGCTTCA ATTTGAAGAG CAGGTGAACA ACATCAAACC TGACATCATG 
GCTGTCAGTA CTGCCTGCGA GGAGATCAAG AAGAGCAAAG GCTTTAGCAA GTTGCTGGAA 
CTTGTGTTGC TAATGGGAAA CTACATGAAT GCTGGCTCCC GGAATGCTCA AACCTTCGGA 
TTTGACCTTA GCTCTCTCTG TAAACTGAAG GATACAAAAT CTGCAGATCA GAAAACCACA 
CTCCTCCATT TCCTGGTAGA TGTATGTGAA GAAAAGCATG CTGACATCCT TCACTTTGTG 
GACGATTTGG CACATTTAGA CAAAGCTAGC AGAGTCTCTG TAGAAATGCT GGAAAAGAAC 
GTGAAGCAGA TGGGAAGGCA GCTTCAACAG CTTGAGAAGA ATTTGGAAAC CTTTCCCCCT 
CCTGAGGACT TGCATGACAA GTTTGTGATA AAGATGTCCA GCTTCGTTAT CAGTGCGAAC 
GAGCAGTATG AAAAACTCTC CACACTACTG GGCAGCATGA CACAATTGTA CCAGAGTATA 
ATGGGCTACT ATGCTGTCGA CATGAAGAAG GTTTCCGTGG AAGAGTTTTT TAATGATCTG 
AACAACTTCA GAACTTCATT TATGCTAGCA TTAAAGGAAA ACATCAAAAA ACGAGAAGCA 
GCAGAAAAGG AGAAACGTGC CAGGATAGCG AAAGAGCGAG CAGAGAAAGA GCGACTTGAA 
CGCCAGCAAG AGAAAAAGCG CTTACTAGAA ATGAAAACTG AGGGAGATGA GACAGGAGTG 
ATGGATAGTC TGCTGGAGGC CTTGCAGTCA GGGGCTGCCT TCCGCGACAG AAGAAAAAGG 
ACACCAAAGC TGAAAGATAT TCGGCAGAGT CTCAGCCCGA TGTCTCAGAG GCCTGTTCTC 
AAAGTTTGTA ACCATGAAAA TCAGAAAATG CAGTTGACAG AAGGGTCACG TCCACACCAC 
AGTATCAATT GCAACTCCAC CAGGACTCCA GTCGCCAAGG AGCTTAATTA TAATCTAGAC 
ACTCATGCGT CTACAGGGAG GATCAAGGCA GTTGAGAAGG AAGCCTGTAA TGCAGAAAGC 
AACAAAAAAA AGGAAATGGA ACTTCTTGGC TCTGTTGCTA AAAGCGAATC AGTTCCTGAA 
GTTGAAGCCC TGCTGGCAAG ATTACGAGCT TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062644.1
CDS	111..3626
Translation	MERHRARALGRDSKSSRRKGLQSAPPAGPYEPGEKRPKLHLNIRTLTDDMLDKFASIRIPGSKKERPPLPHLKTVSGISDSSSLSSETMENNPKALPESEVLKLFEKMMEDMNLNEDKKAPLREKDFGIKKEMVMQYINTASKTGSLRSSRQISPQEFLHELKMGYTDERLFTYLESLRVSLTSHPVSWVQSFGHEGLGLLLDILEKLINGQIQEKVVKKTQHKVIQCLRALMNTQYGLERIMSDKRSLSLLAKAMDPRQPAMMADVVKLLSAVCIVGEESILEEVLEALTSAGEERKIDRFFSIVEGLRHNSVNLQVACMQLINALVTSPDDLDFRLHLRNEFMRCGLKEILPNLKGIKNDGLDIQLKVFDEHKEEDLSEFFHRLEDIRAELDEASDVYSMLWDTVKETRAEGHFLSILQHLLLIRNDRFIREQYFKLIDECVSQIVLHRDGTDPDFTYRKRLDLDLSQFVDVCIDQAKLDEWEEKASEHCKKFEKECTDHQETQAQLQKREAKINELQAELQAFKSQFGALPPGTKIPLQPSVEGEAGPSALPPAPPALSGGVPPPPPPPPPPPPPLPGMPMPFGGPVPPPPPLGFLGGQSSIPLNLPFGLKPKKEFKPEISMRRLNWLKIGPNEMSENCFWIKVNENKYENRDLLCKLENTFCCQEKEKRNTNDFDEKKVIKKRMKELKFLDPKIAQNLSIFLSSFRVPYEKIRTMILEVDETQLSESMIQNLIKHLPDEEQLKSLSQFRSDYNSLCEPEQFAVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKGFSKLLELVLLMGNYMNAGSRNAQTFGFDLSSLCKLKDTKSADQKTTLLHFLVDVCEEKHADILHFVDDLAHLDKASRVSVEMLEKNVKQMGRQLQQLEKNLETFPPPEDLHDKFVIKMSSFVISANEQYEKLSTLLGSMTQLYQSIMGYYAVDMKKVSVEEFFNDLNNFRTSFMLALKENIKKREAAEKEKRARIAKERAEKERLERQQEKKRLLEMKTEGDETGVMDSLLEALQSGAAFRDRRKRTPKLKDIRQSLSPMSQRPVLKVCNHENQKMQLTEGSRPHHSINCNSTRTPVAKELNYNLDTHASTGRIKAVEKEACNAESNKKKEMELLGSVAKSESVPEVEALLARLRAL

Target ORF information:

RefSeq Version	NM_019670.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 3 (Diaph3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019670.2

ORF Insert Sequence:

ATGGAGAGGC ACCGGGCGCG CGCTCTCGGC CGGGACAGCA AGTCGTCGCG GAGGAAGGGC 
TTGCAGTCCG CGCCGCCCGC TGGCCCCTAC GAGCCCGGGG AGAAGCGACC CAAGTTGCAT 
TTAAATATTA GAACACTGAC AGATGATATG CTGGACAAAT TTGCCAGTAT AAGAATTCCA 
GGGAGCAAGA AAGAGAGACC TCCCCTTCCC CACCTGAAGA CTGTGTCTGG GATCAGTGAC 
AGCTCATCAC TGTCCTCAGA GACAATGGAA AACAACCCAA AGGCGCTGCC AGAGAGTGAA 
GTCTTGAAGC TTTTTGAGAA GATGATGGAA GATATGAATT TAAATGAAGA TAAAAAGGCA 
CCATTGCGGG AAAAAGACTT CGGTATCAAA AAAGAAATGG TGATGCAGTA CATTAATACT 
GCTTCTAAGA CAGGAAGTCT TAGAAGTAGC CGACAGATCT CACCTCAGGA ATTTCTCCAT 
GAGCTGAAAA TGGGTTACAC AGACGAGAGA CTTTTCACGT ATCTGGAGTC ACTCCGAGTA 
TCATTGACCA GTCATCCTGT GAGTTGGGTG CAAAGCTTTG GACACGAGGG ACTCGGATTA 
TTGCTGGACA TTTTGGAAAA ACTAATTAAT GGGCAAATCC AAGAAAAAGT TGTGAAGAAG 
ACTCAGCACA AAGTCATCCA GTGTCTGAGA GCCCTGATGA ACACACAGTA TGGCTTAGAA 
AGGATTATGA GTGACAAGAG GAGTCTTTCC TTGTTGGCAA AAGCCATGGA TCCCAGGCAG 
CCCGCTATGA TGGCAGACGT GGTGAAGCTT CTGTCTGCAG TGTGCATTGT CGGCGAGGAA 
AGCATCCTTG AAGAAGTGTT AGAAGCCTTG ACTTCAGCTG GAGAAGAAAG AAAGATTGAC 
AGATTTTTTT CCATTGTGGA AGGCCTCCGG CATAACTCAG TGAACCTGCA AGTTGCTTGT 
ATGCAGCTCA TAAATGCTCT CGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACCTC 
AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATATTGC CAAACTTAAA GGGCATTAAG 
AATGATGGCC TGGATATACA ACTTAAAGTC TTTGATGAGC ACAAAGAAGA AGATTTGAGT 
GAGTTTTTCC ATCGCCTTGA AGACATTAGA GCTGAACTTG ATGAAGCATC TGATGTTTAC 
AGCATGTTAT GGGACACAGT TAAGGAAACT CGAGCAGAGG GACATTTTCT TTCTATTCTT 
CAGCATCTCC TGCTCATTCG CAATGATCGT TTTATAAGAG AGCAGTATTT CAAATTAATT 
GATGAGTGTG TGTCACAGAT TGTATTACAT AGAGATGGAA CGGACCCTGA CTTCACATAC 
AGAAAAAGAC TAGATTTGGA CTTAAGTCAG TTTGTAGATG TTTGCATAGA TCAGGCCAAA 
CTAGATGAGT GGGAAGAGAA AGCATCCGAA CATTGCAAGA AATTTGAAAA AGAGTGTACT 
GACCACCAAG AAACCCAGGC TCAATTGCAG AAAAGAGAGG CAAAGATTAA TGAGCTTCAA 
GCAGAGTTAC AAGCTTTTAA ATCCCAGTTT GGTGCCTTGC CACCTGGTAC AAAAATTCCT 
TTGCAACCTT CAGTAGAAGG TGAAGCTGGC CCTTCAGCCC TTCCTCCTGC CCCACCAGCA 
CTCAGTGGAG GAGTGCCGCC TCCCCCACCG CCCCCTCCTC CACCACCCCC ACCACTCCCA 
GGAATGCCAA TGCCATTTGG TGGCCCTGTA CCACCACCAC CTCCTCTGGG ATTCCTGGGT 
GGGCAAAGCT CTATTCCATT AAACCTGCCA TTTGGTTTGA AACCAAAGAA AGAATTTAAG 
CCTGAAATCA GCATGAGAAG ATTGAATTGG TTAAAGATCG GACCAAATGA AATGTCTGAG 
AACTGCTTCT GGATCAAAGT AAATGAAAAT AAGTATGAAA ATAGGGATTT GCTTTGTAAA 
CTTGAGAACA CTTTTTGTTG CCAAGAAAAA GAGAAAAGGA ATACAAATGA CTTTGATGAG 
AAGAAAGTTA TTAAGAAGAG AATGAAGGAA CTTAAATTTC TAGATCCTAA AATTGCTCAG 
AACCTTTCAA TCTTCCTGAG CTCCTTCCGG GTGCCATATG AGAAAATCAG GACGATGATA 
TTGGAAGTGG ATGAAACACA GTTGTCAGAG TCCATGATTC AGAACTTAAT AAAGCACCTT 
CCTGATGAGG AGCAGTTGAA GTCATTGTCC CAGTTTAGAA GTGACTATAA CAGTTTGTGT 
GAGCCTGAGC AGTTCGCTGT TGTGATGAGC AATGTGAAGA GACTCCGGCC ACGGCTCAGT 
GCTATTCTCT TTAAGCTTCA ATTTGAAGAG CAGGTGAACA ACATCAAACC TGACATCATG 
GCTGTCAGTA CTGCCTGCGA GGAGATCAAG AAGAGCAAAG GCTTTAGCAA GTTGCTGGAA 
CTTGTGTTGC TAATGGGAAA CTACATGAAT GCTGGCTCCC GGAATGCTCA AACCTTCGGA 
TTTGACCTTA GCTCTCTCTG TAAACTGAAG GATACAAAAT CTGCAGATCA GAAAACCACA 
CTCCTCCATT TCCTGGTAGA TGTATGTGAA GAAAAGCATG CTGACATCCT TCACTTTGTG 
GACGATTTGG CACATTTAGA CAAAGCTAGC AGAGTCTCTG TAGAAATGCT GGAAAAGAAC 
GTGAAGCAGA TGGGAAGGCA GCTTCAACAG CTTGAGAAGA ATTTGGAAAC CTTTCCCCCT 
CCTGAGGACT TGCATGACAA GTTTGTGATA AAGATGTCCA GCTTCGTTAT CAGTGCGAAC 
GAGCAGTATG AAAAACTCTC CACACTACTG GGCAGCATGA CACAATTGTA CCAGAGTATA 
ATGGGCTACT ATGCTGTCGA CATGAAGAAG GTTTCCGTGG AAGAGTTTTT TAATGATCTG 
AACAACTTCA GAACTTCATT TATGCTAGCA TTAAAGGAAA ACATCAAAAA ACGAGAAGCA 
GCAGAAAAGG AGAAACGTGC CAGGATAGCG AAAGAGCGAG CAGAGAAAGA GCGACTTGAA 
CGCCAGCAAG AGAAAAAGCG CTTACTAGAA ATGAAAACTG AGGGAGATGA GACAGGAGTG 
ATGGATAGTC TGCTGGAGGC CTTGCAGTCA GGGGCTGCCT TCCGCGACAG AAGAAAAAGG 
ACACCAAAGC TGAAAGATAT TCGGCAGAGT CTCAGCCCGA TGTCTCAGAG GCCTGTTCTC 
AAAGTTTGTA ACCATGAAAA TCAGAAAATG CAGTTGACAG AAGGGTCACG TCCACACCAC 
AGTATCAATT GCAACTCCAC CAGGACTCCA GTCGCCAAGG AGCTTAATTA TAATCTAGAC 
ACTCATGCGT CTACAGGGAG GATCAAGGCA GTTGAGAAGG AAGCCTGTAA TGCAGAAAGC 
AACAAAAAAA AGGAAATGGA ACTTCTTGGC TCTGTTGCTA AAAGCGAATC AGTTCCTGAA 
GTTGAAGCCC TGCTGGCAAG ATTACGAGCT TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diaph3 ( XM_006519312.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519375.1 Mus musculus diaphanous related formin 3 (Diaph3), transcript variant X2, mRNA.

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CloneID	OMu50192
Clone ID Related Accession (Same CDS sequence)	XM_006519312.3 , XM_006519312.4
Accession Version	XM_006519312.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519312.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGC ACCGGGCGCG CGCTCTCGGC CGGGACAGCA AGTCGTCGCG GAGGAAGGGC 
TTGCAGTCCG CGCCGCCCGC TGGCCCCTAC GAGCCCGGGG AGAAGCGACC CAAGTTGCAT 
TTAAATATTA GAACACTGAC AGATGATATG CTGGACAAAT TTGCCAGTAT AAGAATTCCA 
GGGAGCAAGA AAGAGAGACC TCCCCTTCCC CACCTGAAGA CTGTGTCTGG GATCAGTGAC 
AGCTCATCAC TGTCCTCAGA GACAATGGAA AACAACCCAA AGGCGCTGCC AGAGAGTGAA 
GTCTTGAAGC TTTTTGAGAA GATGATGGAA GATATGAATT TAAATGAAGA TAAAAAGGCA 
CCATTGCGGG AAAAAGACTT CGGTATCAAA AAAGAAATGG TGATGCAGTA CATTAATACT 
GCTTCTAAGA CAGGAAGTCT TAGAAGTAGC CGACAGATCT CACCTCAGGA ATTTCTCCAT 
GAGCTGAAAA TGGGTTACAC AGACGAGAGA CTTTTCACGT ATCTGGAGTC ACTCCGAGTA 
TCATTGACCA GTCATCCTGT GAGTTGGGTG CAAAGCTTTG GACACGAGGG ACTCGGATTA 
TTGCTGGACA TTTTGGAAAA ACTAATTAAT GGGCAAATCC AAGAAAAAGT TGTGAAGAAG 
ACTCAGCACA AAGTCATCCA GTGTCTGAGA GCCCTGATGA ACACACAGTA TGGCTTAGAA 
AGGATTATGA GTGACAAGAG GAGTCTTTCC TTGTTGGCAA AAGCCATGGA TCCCAGGCAG 
CCCGCTATGA TGGCAGACGT GGTGAAGCTT CTGTCTGCAG TGTGCATTGT CGGCGAGGAA 
AGCATCCTTG AAGAAGTGTT AGAAGCCTTG ACTTCAGCTG GAGAAGAAAG AAAGATTGAC 
AGATTTTTTT CCATTGTGGA AGGCCTCCGG CATAACTCAG TGAACCTGCA AGTTGCTTGT 
ATGCAGCTCA TAAATGCTCT CGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACCTC 
AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATATTGC CAAACTTAAA GGGCATTAAG 
AATGATGGCC TGGATATACA ACTTAAAGTC TTTGATGAGC ACAAAGAAGA AGATTTGAGT 
GAGTTTTTCC ATCGCCTTGA AGACATTAGA GCTGAACTTG ATGAAGCATC TGATGTTTAC 
AGCATGTTAT GGGACACAGT TAAGGAAACT CGAGCAGAGG GACATTTTCT TTCTATTCTT 
CAGCATCTCC TGCTCATTCG CAATGATCGT TTTATAAGAG AGCAGTATTT CAAATTAATT 
GATGAGTGTG TGTCACAGAT TGTATTACAT AGAGATGGAA CGGACCCTGA CTTCACATAC 
AGAAAAAGAC TAGATTTGGA CTTAAGTCAG TTTGTAGATG TTTGCATAGA TCAGGCCAAA 
CTAGATGAGT GGGAAGAGAA AGCATCCGAA CATTGCAAGA AATTTGAAAA AGAGTGTACT 
GACCACCAAG AAACCCAGGC TCAATTGCAG AAAAGAGAGG CAAAGATTAA TGAGCTTCAA 
GCAGAGTTAC AAGCTTTTAA ATCCCAGTTT GGTGCCTTGC CACCTGGTAC AAAAATTCCT 
TTGCAACCTT CAGTAGAAGG TGAAGCTGGC CCTTCAGCCC TTCCTCCTGC CCCACCAGCA 
CTCAGTGGAG GAGTGCCGCC TCCCCCACCG CCCCCTCCTC CACCACCCCC ACCACTCCCA 
GGAATGCCAA TGCCATTTGG TGGCCCTGTA CCACCACCAC CTCCTCTGGG ATTCCTGGGT 
GGGCAAAGCT CTATTCCATT AAACCTGCCA TTTGGTTTGA AACCAAAGAA AGAATTTAAG 
CCTGAAATCA GCATGAGAAG ATTGAATTGG TTAAAGATCG GACCAAATGA AATGTCTGAG 
AACTGCTTCT GGATCAAAGT AAATGAAAAT AAGTATGAAA ATAGGGATTT GCTTTGTAAA 
CTTGAGAACA CTTTTTGTTG CCAAGAAAAA GAGAAAAGGA ATACAAATGA CTTTGATGAG 
AAGAAAGTTA TTAAGAAGAG AATGAAGGAA CTTAAATTTC TAGATCCTAA AATTGCTCAG 
AACCTTTCAA TCTTCCTGAG CTCCTTCCGG GTGCCATATG AGAAAATCAG GACGATGATA 
TTGGAAGTGG ATGAAACACA GTTGTCAGAG TCCATGATTC AGAACTTAAT AAAGCACCTT 
CCTGATGAGG AGCAGTTGAA GTCATTGTCC CAGTTTAGAA GTGACTATAA CAGTTTGTGT 
GAGCCTGAGC AGTTCGCTGT TGTGATGAGC AATGTGAAGA GACTCCGGCC ACGGCTCAGT 
GCTATTCTCT TTAAGCTTCA ATTTGAAGAG CAGGTGAACA ACATCAAACC TGACATCATG 
GCTGTCAGTA CTGCCTGCGA GGAGATCAAG AAGAGCAAAG GCTTTAGCAA GTTGCTGGAA 
CTTGTGTTGC TAATGGGAAA CTACATGAAT GCTGGCTCCC GGAATGCTCA AACCTTCGGA 
TTTGACCTTA GCTCTCTCTG TAAACTGAAG GATACAAAAT CTGCAGATCA GAAAACCACA 
CTCCTCCATT TCCTGGTAGA TGTATGTGAA GAAAAGCATG CTGACATCCT TCACTTTGTG 
GACGATTTGG CACATTTAGA CAAAGCTAGC AGAGTCTCTG TAGAAATGCT GGAAAAGAAC 
GTGAAGCAGA TGGGAAGGCA GCTTCAACAG CTTGAGAAGA ATTTGGAAAC CTTTCCCCCT 
CCTGAGGACT TGCATGACAA GTTTGTGATA AAGATGTCCA GCTTCGTTAT CAGTGCGAAC 
GAGCAGTATG AAAAACTCTC CACACTACTG GGCAGCATGA CACAATTGTA CCAGAGTATA 
ATGGGCTACT ATGCTGTCGA CATGAAGAAG GTTTCCGTGG AAGAGTTTTT TAATGATCTG 
AACAACTTCA GAACTTCATT TATGCTAGCA TTAAAGGAAA ACATCAAAAA ACGAGAAGCA 
GCAGAAAAGG AGAAACGTGC CAGGATAGCG AAAGAGCGAG CAGAGAAAGA GCGACTTGAA 
CGCCAGCAAG AGAAAAAGCG CTTACTAGAA ATGAAAACTG TCCACAGGAT GCTAACAGAA 
CTTGATCCGA GGAGACATGG CATTTGCAGT GTTCCATCAA GTGTGCACCC CACCATCGCA 
GGACACGTGC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519375.1
CDS	135..3329
Translation	MERHRARALGRDSKSSRRKGLQSAPPAGPYEPGEKRPKLHLNIRTLTDDMLDKFASIRIPGSKKERPPLPHLKTVSGISDSSSLSSETMENNPKALPESEVLKLFEKMMEDMNLNEDKKAPLREKDFGIKKEMVMQYINTASKTGSLRSSRQISPQEFLHELKMGYTDERLFTYLESLRVSLTSHPVSWVQSFGHEGLGLLLDILEKLINGQIQEKVVKKTQHKVIQCLRALMNTQYGLERIMSDKRSLSLLAKAMDPRQPAMMADVVKLLSAVCIVGEESILEEVLEALTSAGEERKIDRFFSIVEGLRHNSVNLQVACMQLINALVTSPDDLDFRLHLRNEFMRCGLKEILPNLKGIKNDGLDIQLKVFDEHKEEDLSEFFHRLEDIRAELDEASDVYSMLWDTVKETRAEGHFLSILQHLLLIRNDRFIREQYFKLIDECVSQIVLHRDGTDPDFTYRKRLDLDLSQFVDVCIDQAKLDEWEEKASEHCKKFEKECTDHQETQAQLQKREAKINELQAELQAFKSQFGALPPGTKIPLQPSVEGEAGPSALPPAPPALSGGVPPPPPPPPPPPPPLPGMPMPFGGPVPPPPPLGFLGGQSSIPLNLPFGLKPKKEFKPEISMRRLNWLKIGPNEMSENCFWIKVNENKYENRDLLCKLENTFCCQEKEKRNTNDFDEKKVIKKRMKELKFLDPKIAQNLSIFLSSFRVPYEKIRTMILEVDETQLSESMIQNLIKHLPDEEQLKSLSQFRSDYNSLCEPEQFAVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKGFSKLLELVLLMGNYMNAGSRNAQTFGFDLSSLCKLKDTKSADQKTTLLHFLVDVCEEKHADILHFVDDLAHLDKASRVSVEMLEKNVKQMGRQLQQLEKNLETFPPPEDLHDKFVIKMSSFVISANEQYEKLSTLLGSMTQLYQSIMGYYAVDMKKVSVEEFFNDLNNFRTSFMLALKENIKKREAAEKEKRARIAKERAEKERLERQQEKKRLLEMKTVHRMLTELDPRRHGICSVPSSVHPTIAGHVP

Target ORF information:

RefSeq Version	XM_006519312.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 3 (Diaph3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519312.3

ORF Insert Sequence:

ATGGAGAGGC ACCGGGCGCG CGCTCTCGGC CGGGACAGCA AGTCGTCGCG GAGGAAGGGC 
TTGCAGTCCG CGCCGCCCGC TGGCCCCTAC GAGCCCGGGG AGAAGCGACC CAAGTTGCAT 
TTAAATATTA GAACACTGAC AGATGATATG CTGGACAAAT TTGCCAGTAT AAGAATTCCA 
GGGAGCAAGA AAGAGAGACC TCCCCTTCCC CACCTGAAGA CTGTGTCTGG GATCAGTGAC 
AGCTCATCAC TGTCCTCAGA GACAATGGAA AACAACCCAA AGGCGCTGCC AGAGAGTGAA 
GTCTTGAAGC TTTTTGAGAA GATGATGGAA GATATGAATT TAAATGAAGA TAAAAAGGCA 
CCATTGCGGG AAAAAGACTT CGGTATCAAA AAAGAAATGG TGATGCAGTA CATTAATACT 
GCTTCTAAGA CAGGAAGTCT TAGAAGTAGC CGACAGATCT CACCTCAGGA ATTTCTCCAT 
GAGCTGAAAA TGGGTTACAC AGACGAGAGA CTTTTCACGT ATCTGGAGTC ACTCCGAGTA 
TCATTGACCA GTCATCCTGT GAGTTGGGTG CAAAGCTTTG GACACGAGGG ACTCGGATTA 
TTGCTGGACA TTTTGGAAAA ACTAATTAAT GGGCAAATCC AAGAAAAAGT TGTGAAGAAG 
ACTCAGCACA AAGTCATCCA GTGTCTGAGA GCCCTGATGA ACACACAGTA TGGCTTAGAA 
AGGATTATGA GTGACAAGAG GAGTCTTTCC TTGTTGGCAA AAGCCATGGA TCCCAGGCAG 
CCCGCTATGA TGGCAGACGT GGTGAAGCTT CTGTCTGCAG TGTGCATTGT CGGCGAGGAA 
AGCATCCTTG AAGAAGTGTT AGAAGCCTTG ACTTCAGCTG GAGAAGAAAG AAAGATTGAC 
AGATTTTTTT CCATTGTGGA AGGCCTCCGG CATAACTCAG TGAACCTGCA AGTTGCTTGT 
ATGCAGCTCA TAAATGCTCT CGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACCTC 
AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATATTGC CAAACTTAAA GGGCATTAAG 
AATGATGGCC TGGATATACA ACTTAAAGTC TTTGATGAGC ACAAAGAAGA AGATTTGAGT 
GAGTTTTTCC ATCGCCTTGA AGACATTAGA GCTGAACTTG ATGAAGCATC TGATGTTTAC 
AGCATGTTAT GGGACACAGT TAAGGAAACT CGAGCAGAGG GACATTTTCT TTCTATTCTT 
CAGCATCTCC TGCTCATTCG CAATGATCGT TTTATAAGAG AGCAGTATTT CAAATTAATT 
GATGAGTGTG TGTCACAGAT TGTATTACAT AGAGATGGAA CGGACCCTGA CTTCACATAC 
AGAAAAAGAC TAGATTTGGA CTTAAGTCAG TTTGTAGATG TTTGCATAGA TCAGGCCAAA 
CTAGATGAGT GGGAAGAGAA AGCATCCGAA CATTGCAAGA AATTTGAAAA AGAGTGTACT 
GACCACCAAG AAACCCAGGC TCAATTGCAG AAAAGAGAGG CAAAGATTAA TGAGCTTCAA 
GCAGAGTTAC AAGCTTTTAA ATCCCAGTTT GGTGCCTTGC CACCTGGTAC AAAAATTCCT 
TTGCAACCTT CAGTAGAAGG TGAAGCTGGC CCTTCAGCCC TTCCTCCTGC CCCACCAGCA 
CTCAGTGGAG GAGTGCCGCC TCCCCCACCG CCCCCTCCTC CACCACCCCC ACCACTCCCA 
GGAATGCCAA TGCCATTTGG TGGCCCTGTA CCACCACCAC CTCCTCTGGG ATTCCTGGGT 
GGGCAAAGCT CTATTCCATT AAACCTGCCA TTTGGTTTGA AACCAAAGAA AGAATTTAAG 
CCTGAAATCA GCATGAGAAG ATTGAATTGG TTAAAGATCG GACCAAATGA AATGTCTGAG 
AACTGCTTCT GGATCAAAGT AAATGAAAAT AAGTATGAAA ATAGGGATTT GCTTTGTAAA 
CTTGAGAACA CTTTTTGTTG CCAAGAAAAA GAGAAAAGGA ATACAAATGA CTTTGATGAG 
AAGAAAGTTA TTAAGAAGAG AATGAAGGAA CTTAAATTTC TAGATCCTAA AATTGCTCAG 
AACCTTTCAA TCTTCCTGAG CTCCTTCCGG GTGCCATATG AGAAAATCAG GACGATGATA 
TTGGAAGTGG ATGAAACACA GTTGTCAGAG TCCATGATTC AGAACTTAAT AAAGCACCTT 
CCTGATGAGG AGCAGTTGAA GTCATTGTCC CAGTTTAGAA GTGACTATAA CAGTTTGTGT 
GAGCCTGAGC AGTTCGCTGT TGTGATGAGC AATGTGAAGA GACTCCGGCC ACGGCTCAGT 
GCTATTCTCT TTAAGCTTCA ATTTGAAGAG CAGGTGAACA ACATCAAACC TGACATCATG 
GCTGTCAGTA CTGCCTGCGA GGAGATCAAG AAGAGCAAAG GCTTTAGCAA GTTGCTGGAA 
CTTGTGTTGC TAATGGGAAA CTACATGAAT GCTGGCTCCC GGAATGCTCA AACCTTCGGA 
TTTGACCTTA GCTCTCTCTG TAAACTGAAG GATACAAAAT CTGCAGATCA GAAAACCACA 
CTCCTCCATT TCCTGGTAGA TGTATGTGAA GAAAAGCATG CTGACATCCT TCACTTTGTG 
GACGATTTGG CACATTTAGA CAAAGCTAGC AGAGTCTCTG TAGAAATGCT GGAAAAGAAC 
GTGAAGCAGA TGGGAAGGCA GCTTCAACAG CTTGAGAAGA ATTTGGAAAC CTTTCCCCCT 
CCTGAGGACT TGCATGACAA GTTTGTGATA AAGATGTCCA GCTTCGTTAT CAGTGCGAAC 
GAGCAGTATG AAAAACTCTC CACACTACTG GGCAGCATGA CACAATTGTA CCAGAGTATA 
ATGGGCTACT ATGCTGTCGA CATGAAGAAG GTTTCCGTGG AAGAGTTTTT TAATGATCTG 
AACAACTTCA GAACTTCATT TATGCTAGCA TTAAAGGAAA ACATCAAAAA ACGAGAAGCA 
GCAGAAAAGG AGAAACGTGC CAGGATAGCG AAAGAGCGAG CAGAGAAAGA GCGACTTGAA 
CGCCAGCAAG AGAAAAAGCG CTTACTAGAA ATGAAAACTG TCCACAGGAT GCTAACAGAA 
CTTGATCCGA GGAGACATGG CATTTGCAGT GTTCCATCAA GTGTGCACCC CACCATCGCA 
GGACACGTGC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diaph3 ( XM_011245123.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243425.1 Mus musculus diaphanous related formin 3 (Diaph3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69653
Clone ID Related Accession (Same CDS sequence)	XM_011245123.2
Accession Version	XM_011245123.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245123.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGC ACCGGGCGCG CGCTCTCGGC CGGGACAGCA AGTCGTCGCG GAGGAAGGGC 
TTGCAGTCCG CGCCGCCCGC TGGCCCCTAC GAGCCCGGGG AGAAGCGACC CAAGTTGCTG 
GACAAATTTG CCAGTATAAG AATTCCAGGG AGCAAGAAAG AGAGACCTCC CCTTCCCCAC 
CTGAAGACTG TGTCTGGGAT CAGTGACAGC TCATCACTGT CCTCAGAGAC AATGGAAAAC 
AACCCAAAGG CGCTGCCAGA GAGTGAAGTC TTGAAGCTTT TTGAGAAGAT GATGGAAGAT 
ATGAATTTAA ATGAAGATAA AAAGGCACCA TTGCGGGAAA AAGACTTCGG TATCAAAAAA 
GAAATGGTGA TGCAGTACAT TAATACTGCT TCTAAGACAG GAAGTCTTAG AAGTAGCCGA 
CAGATCTCAC CTCAGGAATT TCTCCATGAG CTGAAAATGG GTTACACAGA CGAGAGACTT 
TTCACGTATC TGGAGTCACT CCGAGTATCA TTGACCAGTC ATCCTGTGAG TTGGGTGCAA 
AGCTTTGGAC ACGAGGGACT CGGATTATTG CTGGACATTT TGGAAAAACT AATTAATGGG 
CAAATCCAAG AAAAAGTTGT GAAGAAGACT CAGCACAAAG TCATCCAGTG TCTGAGAGCC 
CTGATGAACA CACAGTATGG CTTAGAAAGG ATTATGAGTG ACAAGAGGAG TCTTTCCTTG 
TTGGCAAAAG CCATGGATCC CAGGCAGCCC GCTATGATGG CAGACGTGGT GAAGCTTCTG 
TCTGCAGTGT GCATTGTCGG CGAGGAAAGC ATCCTTGAAG AAGTGTTAGA AGCCTTGACT 
TCAGCTGGAG AAGAAAGAAA GATTGACAGA TTTTTTTCCA TTGTGGAAGG CCTCCGGCAT 
AACTCAGTGA ACCTGCAAGT TGCTTGTATG CAGCTCATAA ATGCTCTCGT TACATCTCCT 
GATGATTTGG ACTTCAGGCT TCACCTCAGA AATGAATTTA TGCGTTGTGG ATTGAAAGAG 
ATATTGCCAA ACTTAAAGGG CATTAAGAAT GATGGCCTGG ATATACAACT TAAAGTCTTT 
GATGAGCACA AAGAAGAAGA TTTGAGTGAG TTTTTCCATC GCCTTGAAGA CATTAGAGCT 
GAACTTGATG AAGCATCTGA TGTTTACAGC ATGTTATGGG ACACAGTTAA GGAAACTCGA 
GCAGAGGGAC ATTTTCTTTC TATTCTTCAG CATCTCCTGC TCATTCGCAA TGATCGTTTT 
ATAAGAGAGC AGTATTTCAA ATTAATTGAT GAGTGTGTGT CACAGATTGT ATTACATAGA 
GATGGAACGG ACCCTGACTT CACATACAGA AAAAGACTAG ATTTGGACTT AAGTCAGTTT 
GTAGATGTTT GCATAGATCA GGCCAAACTA GATGAGTGGG AAGAGAAAGC ATCCGAACAT 
TGCAAGAAAT TTGAAAAAGA GTGTACTGAC CACCAAGAAA CCCAGGCTCA ATTGCAGAAA 
AGAGAGGCAA AGATTAATGA GCTTCAAGCA GAGTTACAAG CTTTTAAATC CCAGTTTGGT 
GCCTTGCCAC CTGGTACAAA AATTCCTTTG CAACCTTCAG TAGAAGGTGA AGCTGGCCCT 
TCAGCCCTTC CTCCTGCCCC ACCAGCACTC AGTGGAGGAG TGCCGCCTCC CCCACCGCCC 
CCTCCTCCAC CACCCCCACC ACTCCCAGGA ATGCCAATGC CATTTGGTGG CCCTGTACCA 
CCACCACCTC CTCTGGGATT CCTGGGTGGG CAAAGCTCTA TTCCATTAAA CCTGCCATTT 
GGTTTGAAAC CAAAGAAAGA ATTTAAGCCT GAAATCAGCA TGAGAAGATT GAATTGGTTA 
AAGATCGGAC CAAATGAAAT GTCTGAGAAC TGCTTCTGGA TCAAAGTAAA TGAAAATAAG 
TATGAAAATA GGGATTTGCT TTGTAAACTT GAGAACACTT TTTGTTGCCA AGAAAAAGAG 
AAAAGGAATA CAAATGACTT TGATGAGAAG AAAGTTATTA AGAAGAGAAT GAAGGAACTT 
AAATTTCTAG ATCCTAAAAT TGCTCAGAAC CTTTCAATCT TCCTGAGCTC CTTCCGGGTG 
CCATATGAGA AAATCAGGAC GATGATATTG GAAGTGGATG AAACACAGTT GTCAGAGTCC 
ATGATTCAGA ACTTAATAAA GCACCTTCCT GATGAGGAGC AGTTGAAGTC ATTGTCCCAG 
TTTAGAAGTG ACTATAACAG TTTGTGTGAG CCTGAGCAGT TCGCTGTTGT GATGAGCAAT 
GTGAAGAGAC TCCGGCCACG GCTCAGTGCT ATTCTCTTTA AGCTTCAATT TGAAGAGCAG 
GTGAACAACA TCAAACCTGA CATCATGGCT GTCAGTACTG CCTGCGAGGA GATCAAGAAG 
AGCAAAGGCT TTAGCAAGTT GCTGGAACTT GTGTTGCTAA TGGGAAACTA CATGAATGCT 
GGCTCCCGGA ATGCTCAAAC CTTCGGATTT GACCTTAGCT CTCTCTGTAA ACTGAAGGAT 
ACAAAATCTG CAGATCAGAA AACCACACTC CTCCATTTCC TGGTAGATGT ATGTGAAGAA 
AAGCATGCTG ACATCCTTCA CTTTGTGGAC GATTTGGCAC ATTTAGACAA AGCTAGCAGA 
GTCTCTGTAG AAATGCTGGA AAAGAACGTG AAGCAGATGG GAAGGCAGCT TCAACAGCTT 
GAGAAGAATT TGGAAACCTT TCCCCCTCCT GAGGACTTGC ATGACAAGTT TGTGATAAAG 
ATGTCCAGCT TCGTTATCAG TGCGAACGAG CAGTATGAAA AACTCTCCAC ACTACTGGGC 
AGCATGACAC AATTGTACCA GAGTATAATG GGCTACTATG CTGTCGACAT GAAGAAGGTT 
TCCGTGGAAG AGTTTTTTAA TGATCTGAAC AACTTCAGAA CTTCATTTAT GCTAGCATTA 
AAGGAAAACA TCAAAAAACG AGAAGCAGCA GAAAAGGAGA AACGTGCCAG GATAGCGAAA 
GAGCGAGCAG AGAAAGAGCG ACTTGAACGC CAGCAAGAGA AAAAGCGCTT ACTAGAAATG 
AAAACTGAGG GAGATGAGAC AGGAGTGATG GATAGTCTGC TGGAGGCCTT GCAGTCAGGG 
GCTGCCTTCC GCGACAGAAG AAAAAGGACA CCAAAGCTGA AAGATATTCG GCAGAGTCTC 
AGCCCGATGT CTCAGAGGCC TGTTCTCAAA GTTTGTAACC ATGAAAATCA GAAAATGCAG 
TTGACAGAAG GGTCACGTCC ACACCACAGT ATCAATTGCA ACTCCACCAG GACTCCAGTC 
GCCAAGGAGC TTAATTATAA TCTAGACACT CATGCGTCTA CAGGGAGGAT CAAGGCAGTT 
GAGAAGGAAG CCTGTAATGC AGAAAGCAAC AAAAAAAAGG AAATGGAACT TCTTGGCTCT 
GTTGCTAAAA GCGAATCAGT TCCTGAAGTT GAAGCCCTGC TGGCAAGATT ACGAGCTTTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243425.1
CDS	122..3604
Translation	MERHRARALGRDSKSSRRKGLQSAPPAGPYEPGEKRPKLLDKFASIRIPGSKKERPPLPHLKTVSGISDSSSLSSETMENNPKALPESEVLKLFEKMMEDMNLNEDKKAPLREKDFGIKKEMVMQYINTASKTGSLRSSRQISPQEFLHELKMGYTDERLFTYLESLRVSLTSHPVSWVQSFGHEGLGLLLDILEKLINGQIQEKVVKKTQHKVIQCLRALMNTQYGLERIMSDKRSLSLLAKAMDPRQPAMMADVVKLLSAVCIVGEESILEEVLEALTSAGEERKIDRFFSIVEGLRHNSVNLQVACMQLINALVTSPDDLDFRLHLRNEFMRCGLKEILPNLKGIKNDGLDIQLKVFDEHKEEDLSEFFHRLEDIRAELDEASDVYSMLWDTVKETRAEGHFLSILQHLLLIRNDRFIREQYFKLIDECVSQIVLHRDGTDPDFTYRKRLDLDLSQFVDVCIDQAKLDEWEEKASEHCKKFEKECTDHQETQAQLQKREAKINELQAELQAFKSQFGALPPGTKIPLQPSVEGEAGPSALPPAPPALSGGVPPPPPPPPPPPPPLPGMPMPFGGPVPPPPPLGFLGGQSSIPLNLPFGLKPKKEFKPEISMRRLNWLKIGPNEMSENCFWIKVNENKYENRDLLCKLENTFCCQEKEKRNTNDFDEKKVIKKRMKELKFLDPKIAQNLSIFLSSFRVPYEKIRTMILEVDETQLSESMIQNLIKHLPDEEQLKSLSQFRSDYNSLCEPEQFAVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKGFSKLLELVLLMGNYMNAGSRNAQTFGFDLSSLCKLKDTKSADQKTTLLHFLVDVCEEKHADILHFVDDLAHLDKASRVSVEMLEKNVKQMGRQLQQLEKNLETFPPPEDLHDKFVIKMSSFVISANEQYEKLSTLLGSMTQLYQSIMGYYAVDMKKVSVEEFFNDLNNFRTSFMLALKENIKKREAAEKEKRARIAKERAEKERLERQQEKKRLLEMKTEGDETGVMDSLLEALQSGAAFRDRRKRTPKLKDIRQSLSPMSQRPVLKVCNHENQKMQLTEGSRPHHSINCNSTRTPVAKELNYNLDTHASTGRIKAVEKEACNAESNKKKEMELLGSVAKSESVPEVEALLARLRAL

Target ORF information:

RefSeq Version	XM_011245123.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 3 (Diaph3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245123.2

ORF Insert Sequence:

ATGGAGAGGC ACCGGGCGCG CGCTCTCGGC CGGGACAGCA AGTCGTCGCG GAGGAAGGGC 
TTGCAGTCCG CGCCGCCCGC TGGCCCCTAC GAGCCCGGGG AGAAGCGACC CAAGTTGCTG 
GACAAATTTG CCAGTATAAG AATTCCAGGG AGCAAGAAAG AGAGACCTCC CCTTCCCCAC 
CTGAAGACTG TGTCTGGGAT CAGTGACAGC TCATCACTGT CCTCAGAGAC AATGGAAAAC 
AACCCAAAGG CGCTGCCAGA GAGTGAAGTC TTGAAGCTTT TTGAGAAGAT GATGGAAGAT 
ATGAATTTAA ATGAAGATAA AAAGGCACCA TTGCGGGAAA AAGACTTCGG TATCAAAAAA 
GAAATGGTGA TGCAGTACAT TAATACTGCT TCTAAGACAG GAAGTCTTAG AAGTAGCCGA 
CAGATCTCAC CTCAGGAATT TCTCCATGAG CTGAAAATGG GTTACACAGA CGAGAGACTT 
TTCACGTATC TGGAGTCACT CCGAGTATCA TTGACCAGTC ATCCTGTGAG TTGGGTGCAA 
AGCTTTGGAC ACGAGGGACT CGGATTATTG CTGGACATTT TGGAAAAACT AATTAATGGG 
CAAATCCAAG AAAAAGTTGT GAAGAAGACT CAGCACAAAG TCATCCAGTG TCTGAGAGCC 
CTGATGAACA CACAGTATGG CTTAGAAAGG ATTATGAGTG ACAAGAGGAG TCTTTCCTTG 
TTGGCAAAAG CCATGGATCC CAGGCAGCCC GCTATGATGG CAGACGTGGT GAAGCTTCTG 
TCTGCAGTGT GCATTGTCGG CGAGGAAAGC ATCCTTGAAG AAGTGTTAGA AGCCTTGACT 
TCAGCTGGAG AAGAAAGAAA GATTGACAGA TTTTTTTCCA TTGTGGAAGG CCTCCGGCAT 
AACTCAGTGA ACCTGCAAGT TGCTTGTATG CAGCTCATAA ATGCTCTCGT TACATCTCCT 
GATGATTTGG ACTTCAGGCT TCACCTCAGA AATGAATTTA TGCGTTGTGG ATTGAAAGAG 
ATATTGCCAA ACTTAAAGGG CATTAAGAAT GATGGCCTGG ATATACAACT TAAAGTCTTT 
GATGAGCACA AAGAAGAAGA TTTGAGTGAG TTTTTCCATC GCCTTGAAGA CATTAGAGCT 
GAACTTGATG AAGCATCTGA TGTTTACAGC ATGTTATGGG ACACAGTTAA GGAAACTCGA 
GCAGAGGGAC ATTTTCTTTC TATTCTTCAG CATCTCCTGC TCATTCGCAA TGATCGTTTT 
ATAAGAGAGC AGTATTTCAA ATTAATTGAT GAGTGTGTGT CACAGATTGT ATTACATAGA 
GATGGAACGG ACCCTGACTT CACATACAGA AAAAGACTAG ATTTGGACTT AAGTCAGTTT 
GTAGATGTTT GCATAGATCA GGCCAAACTA GATGAGTGGG AAGAGAAAGC ATCCGAACAT 
TGCAAGAAAT TTGAAAAAGA GTGTACTGAC CACCAAGAAA CCCAGGCTCA ATTGCAGAAA 
AGAGAGGCAA AGATTAATGA GCTTCAAGCA GAGTTACAAG CTTTTAAATC CCAGTTTGGT 
GCCTTGCCAC CTGGTACAAA AATTCCTTTG CAACCTTCAG TAGAAGGTGA AGCTGGCCCT 
TCAGCCCTTC CTCCTGCCCC ACCAGCACTC AGTGGAGGAG TGCCGCCTCC CCCACCGCCC 
CCTCCTCCAC CACCCCCACC ACTCCCAGGA ATGCCAATGC CATTTGGTGG CCCTGTACCA 
CCACCACCTC CTCTGGGATT CCTGGGTGGG CAAAGCTCTA TTCCATTAAA CCTGCCATTT 
GGTTTGAAAC CAAAGAAAGA ATTTAAGCCT GAAATCAGCA TGAGAAGATT GAATTGGTTA 
AAGATCGGAC CAAATGAAAT GTCTGAGAAC TGCTTCTGGA TCAAAGTAAA TGAAAATAAG 
TATGAAAATA GGGATTTGCT TTGTAAACTT GAGAACACTT TTTGTTGCCA AGAAAAAGAG 
AAAAGGAATA CAAATGACTT TGATGAGAAG AAAGTTATTA AGAAGAGAAT GAAGGAACTT 
AAATTTCTAG ATCCTAAAAT TGCTCAGAAC CTTTCAATCT TCCTGAGCTC CTTCCGGGTG 
CCATATGAGA AAATCAGGAC GATGATATTG GAAGTGGATG AAACACAGTT GTCAGAGTCC 
ATGATTCAGA ACTTAATAAA GCACCTTCCT GATGAGGAGC AGTTGAAGTC ATTGTCCCAG 
TTTAGAAGTG ACTATAACAG TTTGTGTGAG CCTGAGCAGT TCGCTGTTGT GATGAGCAAT 
GTGAAGAGAC TCCGGCCACG GCTCAGTGCT ATTCTCTTTA AGCTTCAATT TGAAGAGCAG 
GTGAACAACA TCAAACCTGA CATCATGGCT GTCAGTACTG CCTGCGAGGA GATCAAGAAG 
AGCAAAGGCT TTAGCAAGTT GCTGGAACTT GTGTTGCTAA TGGGAAACTA CATGAATGCT 
GGCTCCCGGA ATGCTCAAAC CTTCGGATTT GACCTTAGCT CTCTCTGTAA ACTGAAGGAT 
ACAAAATCTG CAGATCAGAA AACCACACTC CTCCATTTCC TGGTAGATGT ATGTGAAGAA 
AAGCATGCTG ACATCCTTCA CTTTGTGGAC GATTTGGCAC ATTTAGACAA AGCTAGCAGA 
GTCTCTGTAG AAATGCTGGA AAAGAACGTG AAGCAGATGG GAAGGCAGCT TCAACAGCTT 
GAGAAGAATT TGGAAACCTT TCCCCCTCCT GAGGACTTGC ATGACAAGTT TGTGATAAAG 
ATGTCCAGCT TCGTTATCAG TGCGAACGAG CAGTATGAAA AACTCTCCAC ACTACTGGGC 
AGCATGACAC AATTGTACCA GAGTATAATG GGCTACTATG CTGTCGACAT GAAGAAGGTT 
TCCGTGGAAG AGTTTTTTAA TGATCTGAAC AACTTCAGAA CTTCATTTAT GCTAGCATTA 
AAGGAAAACA TCAAAAAACG AGAAGCAGCA GAAAAGGAGA AACGTGCCAG GATAGCGAAA 
GAGCGAGCAG AGAAAGAGCG ACTTGAACGC CAGCAAGAGA AAAAGCGCTT ACTAGAAATG 
AAAACTGAGG GAGATGAGAC AGGAGTGATG GATAGTCTGC TGGAGGCCTT GCAGTCAGGG 
GCTGCCTTCC GCGACAGAAG AAAAAGGACA CCAAAGCTGA AAGATATTCG GCAGAGTCTC 
AGCCCGATGT CTCAGAGGCC TGTTCTCAAA GTTTGTAACC ATGAAAATCA GAAAATGCAG 
TTGACAGAAG GGTCACGTCC ACACCACAGT ATCAATTGCA ACTCCACCAG GACTCCAGTC 
GCCAAGGAGC TTAATTATAA TCTAGACACT CATGCGTCTA CAGGGAGGAT CAAGGCAGTT 
GAGAAGGAAG CCTGTAATGC AGAAAGCAAC AAAAAAAAGG AAATGGAACT TCTTGGCTCT 
GTTGCTAAAA GCGAATCAGT TCCTGAAGTT GAAGCCCTGC TGGCAAGATT ACGAGCTTTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histone deacetylase 6 regulates cytokinesis and erythrocyte enucleation through deacetylation of formin protein mDia2.
Haematologica102(6)984-994(2017 Jun)
Li X,Mei Y,Yan B,Vitriol E,Huang S,Ji P,Qiu Y

Quantitative Proteomics Illuminates a Functional Interaction between mDia2 and the Proteasome.
Journal of proteome research15(12)4624-4637(2016 Dec)
Isogai T,van der Kammen R,Bleijerveld OB,Goerdayal SS,Argenzio E,Altelaar AF,Innocenti M

Lack of Diaph3 relaxes the spindle checkpoint causing the loss of neural progenitors.
Nature communications713509(2016 Nov)
Damiani D,Goffinet AM,Alberts A,Tissir F

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Diaph3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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