Trpc4ap cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trpc4ap
Entrez Gene ID	56407
Full Name	transient receptor potential cation channel, subfamily C, member 4 associated protein
Synonyms	4833429F06Rik,D2Ertd113e,Trrp4ap,mFLJ00177,truss
General protein information	Preferred Names transient receptor potential cation channel, subfamily C, member 4 associated protein Names short transient receptor potential channel 4-associated protein TNF-receptor ubiquitous scaffolding/signaling protein Trp4-associated protein TAP1 rabex-5/Rin2-interacting protein transient receptor protein 4, associated protein trpc4-associated protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 H1\|2 77.26 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_019828.2	NP_062802.2	short transient receptor potential channel 4-associated protein isoform 2
NM_001163452.1	NP_001156924.1	short transient receptor potential channel 4-associated protein isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TRPC4AP	XP_851670.2
Homo sapiens (human)	TRPC4AP	NP_056453.1
Bos taurus (cattle)	TRPC4AP	NP_001193759.1
Xenopus tropicalis (tropical clawed frog)	trpc4ap	NP_001120170.1
Pan troglodytes (chimpanzee)	TRPC4AP	XP_003316922.1
Mus musculus (house mouse)	Trpc4ap	NP_001156924.1
Danio rerio (zebrafish)	trpc4apa	NP_571644.1
Rattus norvegicus (Norway rat)	Trpc4ap	NP_001094218.1
Gallus gallus (chicken)	TRPC4AP	NP_001034406.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.
DiTommaso T, Jones LK, Cottle DL, , Gerdin AK, Vancollie VE, Watt FM, Ramirez-Solis R, Bradley A, Steel KP, Sundberg JP, White JK, Smyth IM
PLoS genetics10(10)e1004705(2014 Oct)

TRUSS, a novel tumor necrosis factor receptor 1 scaffolding protein that mediates activation of the transcription factor NF-kappaB.
Soond SM, Terry JL, Colbert JD, Riches DW
Molecular and cellular biology23(22)8334-44(2003 Nov)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

cloning and characterization of tumor necrosis factor receptor 1 (TNF-R1)-associated ubiquitous scaffolding and signaling protein (TRUSS)
Title: TRUSS, a novel tumor necrosis factor receptor 1 scaffolding protein that mediates activation of the transcription factor NF-kappaB.

The following Trpc4ap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trpc4ap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11462	NM_019828.2 Latest version!	Mus musculus transient receptor potential cation channel, subfamily C, member 4 associated protein (Trpc4ap), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu09027	NM_001163452.1 Latest version!	Mus musculus transient receptor potential cation channel, subfamily C, member 4 associated protein (Trpc4ap), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trpc4ap ( NM_019828.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062802.2 Mus musculus transient receptor potential cation channel, subfamily C, member 4 associated protein (Trpc4ap), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu11462
Clone ID Related Accession (Same CDS sequence)	NM_019828.2
Accession Version	NM_019828.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Mus musculus(house mouse)
Product	short transient receptor potential channel 4-associated protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL844852.11, AK171404.1 and BC057330.1. On Jul 21, 2009 this sequence version replaced NM_019828.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini as isoform 1. ##Evidence-Data-START## Transcript exon combination :: AF130458.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG CGCCCGCAGC GGCTGGAGCT GGGGCCAGCC GGGGGAGGCG GTTGGCAGCT 
ACCGCGGCGG CGTGGGGCGG CTGGGGAGGT CGACCGCGGC CGGGTAACAT CCTGCTGCAG 
CTGCGGCAGG GCCAGCTCAC CGGCCGAGGC CTGGTCCGGG CGGTGCAGTT CACTGAGACC 
TTTTTGACCG AGAGGGACAA ATTGTCCAAA TGGAGTGGAA TTCCTCAGTT GCTCCTTAAG 
CTGTATGCCA CCAGCCATCT GCACAGTGAC TTCGTTGAGT GCCAAAGCAT TCTCAAGGAG 
ATTTCTCCTC TTCTCTCTAT GGAGGCTATG GCGTTTGTAA CTGAAGACAG GAAATTTACT 
CAAGAAGCTA CATACCCAAA TACATATATT TTTGACTTAT TTGGAGGTGT TGATCTTCTT 
GTAGAAATTC TTATGAGGCC TACCATCTCT ATCCGGGGAC AGAAACTAAA AATAAGTGAC 
GAGATGTCGA AGGACTGCTT GAGCATCCTG TACAATACCT GTGTCTGCAC GGAAGGAGTA 
ACGAAGCGTT TGGCAGAAAA GAATGATTTT GTGATTTTTC TGTTCACACT GATGACAAGT 
AAGAAGACAT TCTTACAAAC AGCGACTCTC ATTGAAGACA TTTTGGGTGT TAAGAAGGAA 
ATGATCCGGC TAGATGAAGT TCCCAATCTG AGTTCCTTAG TATCCAATTT CGATCAGCAG 
CAGCTTGCTA ACTTCTGCAG GATCCTGGCT GTCACCATTT CAGAGATGGA CACGGGGAAT 
GACGACAAAC ACACGCTTCT TGCCAAAAAT GCTCAGCAGA AGAAGAGTTT GAGTTTGGGG 
CCTTCTGCAG CTGAAATCAA CCAAGCTGCT CTTCTCAGCA TCCCTGGCTT TGTGGAACGG 
CTTTGCAAAC TGGCAACTAG GAAAGTGTCA GAGTCAACGG GCACAGCCAG CTTCCTTCAA 
GAACTGGAGG AGTGGTACAC ATGGCTCGAC AATGCGTTGG TGCTAGATGC CCTGATGCGA 
GTGGCCAATG AGGAGTCTGA ACACAATCAA GGCACTTCGG AGGAGGGTGG GCTGCCACAC 
ACCTCGGCCA GGGCGCAGCT GCCCCAGTCC ATGAAGATTA TGCATGAGAT TATGTACAAA 
CTGGAAGTGC TTTATGTCCT CTGCGTGCTG CTGATGGGGC GTCAGCGAAA CCAGGTCCAC 
AGGATGATTG CTGAGTTCAA GTTGATCCCA GGACTCAATA ATTTGTTCGA CAAACTGATT 
TGGAGGAAGC ATTCAGCATC TGCCCTTGTC CTCCATGGCC ACAACCAGAA CTGTGACTGT 
AGCCCGGACA TCACCTTGAA GATCCAGTTT TTGAGACTTC TCCAGAGTTT CAGTGACCAC 
CATGAGAACA AGTACTTGCT GCTCAACAAC CAGGAGCTAA ATGAGCTCAG TGCCATCTCG 
CTGAAAGCCA ACATCCCTGA GGTGGAGGCT GTCCTCAACA CTGACAGGAG TCTGGTGTGT 
GACGGCAAGA GGGGCCTACT AACTCGGCTG CTGCAGGTCA TGAAGAAGGA GCCAGCGGAG 
TCTTCATTCA GGTTTTGGCA AGCCCGGGCT GTGGAAAGTT TCCTCCGAGG GACCACCTCC 
TATGCAGACC AGATGTTCCT GCTGAAGCGA GGCCTTCTGG AGCACATCTT ATACTGCATT 
GTGGACAGTG AGTGCAAGTC TAGGGATGTA CTCCAGAGCT ACTTTGATCT CCTGGGAGAG 
CTGATGAAGT TCAACGTCGA TGCATTCAAG AGATTCAATA AATATATCAA CACTGATGCA 
AAGTTCCAGG TGTTCCTGAA ACAGATCAAC AGCTCGCTAG TGGACTCCAA CATGCTGGTG 
CGCTGTGTCA CTCTGTCACT GGACCGATTT GAAAACCAGG TGGACATGAA AGTGGCCGAG 
GTCCTGTCCG AGTGCCGCCT GCTTGCCTAC ATATCCCAGG TGCCCACGCA AATGTCCTTC 
CTCTTCCGCC TCATCAATAT CATCCATGTG CAGACGCTAA CCCAGGAGAA CGTCAGCTGC 
CTCAACACCA GCCTGGTAAT CCTGATGCTG GCCCGACGGA AAGAGCGGCT GCCCCTGTAC 
CTGCGACTGC TACAGCGAAT GGAGCACAGC AAGAAATACC CCGGCTTCCT GCTCAACAAC 
TTCCACAACC TGCTGCGCTT TTGGCAGCAG CACTACCTGC ACAAGGATAA GGACAGCACC 
TGCCTGGAGA ATAGCTCCTG TATCAGCTTC TCGTACTGGA AGGAGACAGT GTCCATCTTG 
TTGAACCCAG ACCGCCAGTC GCCCTCTGCC CTCGTCAGCT ACATTGAGGA GCCCTACATG 
GACATAGACA GAGACTTCAC TGAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062802.2
CDS	79..2448
Translation	MAAAPAAAGAGASRGRRLAATAAAWGGWGGRPRPGNILLQLRQGQLTGRGLVRAVQFTETFLTERDKLSKWSGIPQLLLKLYATSHLHSDFVECQSILKEISPLLSMEAMAFVTEDRKFTQEATYPNTYIFDLFGGVDLLVEILMRPTISIRGQKLKISDEMSKDCLSILYNTCVCTEGVTKRLAEKNDFVIFLFTLMTSKKTFLQTATLIEDILGVKKEMIRLDEVPNLSSLVSNFDQQQLANFCRILAVTISEMDTGNDDKHTLLAKNAQQKKSLSLGPSAAEINQAALLSIPGFVERLCKLATRKVSESTGTASFLQELEEWYTWLDNALVLDALMRVANEESEHNQGTSEEGGLPHTSARAQLPQSMKIMHEIMYKLEVLYVLCVLLMGRQRNQVHRMIAEFKLIPGLNNLFDKLIWRKHSASALVLHGHNQNCDCSPDITLKIQFLRLLQSFSDHHENKYLLLNNQELNELSAISLKANIPEVEAVLNTDRSLVCDGKRGLLTRLLQVMKKEPAESSFRFWQARAVESFLRGTTSYADQMFLLKRGLLEHILYCIVDSECKSRDVLQSYFDLLGELMKFNVDAFKRFNKYINTDAKFQVFLKQINSSLVDSNMLVRCVTLSLDRFENQVDMKVAEVLSECRLLAYISQVPTQMSFLFRLINIIHVQTLTQENVSCLNTSLVILMLARRKERLPLYLRLLQRMEHSKKYPGFLLNNFHNLLRFWQQHYLHKDKDSTCLENSSCISFSYWKETVSILLNPDRQSPSALVSYIEEPYMDIDRDFTEE

Target ORF information:

RefSeq Version	NM_019828.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily C, member 4 associated protein (Trpc4ap), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019828.2

ORF Insert Sequence:

ATGGCGGCAG CGCCCGCAGC GGCTGGAGCT GGGGCCAGCC GGGGGAGGCG GTTGGCAGCT 
ACCGCGGCGG CGTGGGGCGG CTGGGGAGGT CGACCGCGGC CGGGTAACAT CCTGCTGCAG 
CTGCGGCAGG GCCAGCTCAC CGGCCGAGGC CTGGTCCGGG CGGTGCAGTT CACTGAGACC 
TTTTTGACCG AGAGGGACAA ATTGTCCAAA TGGAGTGGAA TTCCTCAGTT GCTCCTTAAG 
CTGTATGCCA CCAGCCATCT GCACAGTGAC TTCGTTGAGT GCCAAAGCAT TCTCAAGGAG 
ATTTCTCCTC TTCTCTCTAT GGAGGCTATG GCGTTTGTAA CTGAAGACAG GAAATTTACT 
CAAGAAGCTA CATACCCAAA TACATATATT TTTGACTTAT TTGGAGGTGT TGATCTTCTT 
GTAGAAATTC TTATGAGGCC TACCATCTCT ATCCGGGGAC AGAAACTAAA AATAAGTGAC 
GAGATGTCGA AGGACTGCTT GAGCATCCTG TACAATACCT GTGTCTGCAC GGAAGGAGTA 
ACGAAGCGTT TGGCAGAAAA GAATGATTTT GTGATTTTTC TGTTCACACT GATGACAAGT 
AAGAAGACAT TCTTACAAAC AGCGACTCTC ATTGAAGACA TTTTGGGTGT TAAGAAGGAA 
ATGATCCGGC TAGATGAAGT TCCCAATCTG AGTTCCTTAG TATCCAATTT CGATCAGCAG 
CAGCTTGCTA ACTTCTGCAG GATCCTGGCT GTCACCATTT CAGAGATGGA CACGGGGAAT 
GACGACAAAC ACACGCTTCT TGCCAAAAAT GCTCAGCAGA AGAAGAGTTT GAGTTTGGGG 
CCTTCTGCAG CTGAAATCAA CCAAGCTGCT CTTCTCAGCA TCCCTGGCTT TGTGGAACGG 
CTTTGCAAAC TGGCAACTAG GAAAGTGTCA GAGTCAACGG GCACAGCCAG CTTCCTTCAA 
GAACTGGAGG AGTGGTACAC ATGGCTCGAC AATGCGTTGG TGCTAGATGC CCTGATGCGA 
GTGGCCAATG AGGAGTCTGA ACACAATCAA GGCACTTCGG AGGAGGGTGG GCTGCCACAC 
ACCTCGGCCA GGGCGCAGCT GCCCCAGTCC ATGAAGATTA TGCATGAGAT TATGTACAAA 
CTGGAAGTGC TTTATGTCCT CTGCGTGCTG CTGATGGGGC GTCAGCGAAA CCAGGTCCAC 
AGGATGATTG CTGAGTTCAA GTTGATCCCA GGACTCAATA ATTTGTTCGA CAAACTGATT 
TGGAGGAAGC ATTCAGCATC TGCCCTTGTC CTCCATGGCC ACAACCAGAA CTGTGACTGT 
AGCCCGGACA TCACCTTGAA GATCCAGTTT TTGAGACTTC TCCAGAGTTT CAGTGACCAC 
CATGAGAACA AGTACTTGCT GCTCAACAAC CAGGAGCTAA ATGAGCTCAG TGCCATCTCG 
CTGAAAGCCA ACATCCCTGA GGTGGAGGCT GTCCTCAACA CTGACAGGAG TCTGGTGTGT 
GACGGCAAGA GGGGCCTACT AACTCGGCTG CTGCAGGTCA TGAAGAAGGA GCCAGCGGAG 
TCTTCATTCA GGTTTTGGCA AGCCCGGGCT GTGGAAAGTT TCCTCCGAGG GACCACCTCC 
TATGCAGACC AGATGTTCCT GCTGAAGCGA GGCCTTCTGG AGCACATCTT ATACTGCATT 
GTGGACAGTG AGTGCAAGTC TAGGGATGTA CTCCAGAGCT ACTTTGATCT CCTGGGAGAG 
CTGATGAAGT TCAACGTCGA TGCATTCAAG AGATTCAATA AATATATCAA CACTGATGCA 
AAGTTCCAGG TGTTCCTGAA ACAGATCAAC AGCTCGCTAG TGGACTCCAA CATGCTGGTG 
CGCTGTGTCA CTCTGTCACT GGACCGATTT GAAAACCAGG TGGACATGAA AGTGGCCGAG 
GTCCTGTCCG AGTGCCGCCT GCTTGCCTAC ATATCCCAGG TGCCCACGCA AATGTCCTTC 
CTCTTCCGCC TCATCAATAT CATCCATGTG CAGACGCTAA CCCAGGAGAA CGTCAGCTGC 
CTCAACACCA GCCTGGTAAT CCTGATGCTG GCCCGACGGA AAGAGCGGCT GCCCCTGTAC 
CTGCGACTGC TACAGCGAAT GGAGCACAGC AAGAAATACC CCGGCTTCCT GCTCAACAAC 
TTCCACAACC TGCTGCGCTT TTGGCAGCAG CACTACCTGC ACAAGGATAA GGACAGCACC 
TGCCTGGAGA ATAGCTCCTG TATCAGCTTC TCGTACTGGA AGGAGACAGT GTCCATCTTG 
TTGAACCCAG ACCGCCAGTC GCCCTCTGCC CTCGTCAGCT ACATTGAGGA GCCCTACATG 
GACATAGACA GAGACTTCAC TGAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpc4ap ( NM_001163452.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156924.1 Mus musculus transient receptor potential cation channel, subfamily C, member 4 associated protein (Trpc4ap), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09027
Clone ID Related Accession (Same CDS sequence)	NM_001163452.1
Accession Version	NM_001163452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Mus musculus(house mouse)
Product	short transient receptor potential channel 4-associated protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL844852.11, AK171404.1 and BC057330.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC057330.1, AK220239.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG CGCCCGCAGC GGCTGGAGCT GGGGCCAGCC GGGGGAGGCG GTTGGCAGCT 
ACCGCGGCGG CGTGGGGCGG CTGGGGAGGT CGACCGCGGC CGGGTAACAT CCTGCTGCAG 
CTGCGGCAGG GCCAGCTCAC CGGCCGAGGC CTGGTCCGGG CGGTGCAGTT CACTGAGACC 
TTTTTGACCG AGAGGGACAA ATTGTCCAAA TGGAGTGGAA TTCCTCAGTT GCTCCTTAAG 
CTGTATGCCA CCAGCCATCT GCACAGTGAC TTCGTTGAGT GCCAAAGCAT TCTCAAGGAG 
ATTTCTCCTC TTCTCTCTAT GGAGGCTATG GCGTTTGTAA CTGAAGACAG GAAATTTACT 
CAAGAAGCTA CATACCCAAA TACATATATT TTTGACTTAT TTGGAGGTGT TGATCTTCTT 
GTAGAAATTC TTATGAGGCC TACCATCTCT ATCCGGGGAC AGAAACTAAA AATAAGTGAC 
GAGATGTCGA AGGACTGCTT GAGCATCCTG TACAATACCT GTGTCTGCAC GGAAGGAGTA 
ACGAAGCGTT TGGCAGAAAA GAATGATTTT GTGATTTTTC TGTTCACACT GATGACAAGT 
AAGAAGACAT TCTTACAAAC AGCGACTCTC ATTGAAGACA TTTTGGGTGT TAAGAAGGAA 
ATGATCCGGC TAGATGAAGT TCCCAATCTG AGTTCCTTAG TATCCAATTT CGATCAGCAG 
CAGCTTGCTA ACTTCTGCAG GATCCTGGCT GTCACCATTT CAGAGATGGA CACGGGGAAT 
GACGACAAAC ACACGCTTCT TGCCAAAAAT GCTCAGCAGA AGAAGAGTTT GAGTTTGGGG 
CCTTCTGCAG CTGAAATCAA CCAAGCTGCT CTTCTCAGCA TCCCTGGCTT TGTGGAACGG 
CTTTGCAAAC TGGCAACTAG GAAAGTGTCA GAGTCAACGG GCACAGCCAG CTTCCTTCAA 
GAACTGGAGG AGTGGTACAC ATGGCTCGAC AATGCGTTGG TGCTAGATGC CCTGATGCGA 
GTGGCCAATG AGGAGTCTGA ACACAATCAA GCCCCCACTG TGTTCCCTTC TCTAGGCACT 
TCGGAGGAGG GTGGGCTGCC ACACACCTCG GCCAGGGCGC AGCTGCCCCA GTCCATGAAG 
ATTATGCATG AGATTATGTA CAAACTGGAA GTGCTTTATG TCCTCTGCGT GCTGCTGATG 
GGGCGTCAGC GAAACCAGGT CCACAGGATG ATTGCTGAGT TCAAGTTGAT CCCAGGACTC 
AATAATTTGT TCGACAAACT GATTTGGAGG AAGCATTCAG CATCTGCCCT TGTCCTCCAT 
GGCCACAACC AGAACTGTGA CTGTAGCCCG GACATCACCT TGAAGATCCA GTTTTTGAGA 
CTTCTCCAGA GTTTCAGTGA CCACCATGAG AACAAGTACT TGCTGCTCAA CAACCAGGAG 
CTAAATGAGC TCAGTGCCAT CTCGCTGAAA GCCAACATCC CTGAGGTGGA GGCTGTCCTC 
AACACTGACA GGAGTCTGGT GTGTGACGGC AAGAGGGGCC TACTAACTCG GCTGCTGCAG 
GTCATGAAGA AGGAGCCAGC GGAGTCTTCA TTCAGGTTTT GGCAAGCCCG GGCTGTGGAA 
AGTTTCCTCC GAGGGACCAC CTCCTATGCA GACCAGATGT TCCTGCTGAA GCGAGGCCTT 
CTGGAGCACA TCTTATACTG CATTGTGGAC AGTGAGTGCA AGTCTAGGGA TGTACTCCAG 
AGCTACTTTG ATCTCCTGGG AGAGCTGATG AAGTTCAACG TCGATGCATT CAAGAGATTC 
AATAAATATA TCAACACTGA TGCAAAGTTC CAGGTGTTCC TGAAACAGAT CAACAGCTCG 
CTAGTGGACT CCAACATGCT GGTGCGCTGT GTCACTCTGT CACTGGACCG ATTTGAAAAC 
CAGGTGGACA TGAAAGTGGC CGAGGTCCTG TCCGAGTGCC GCCTGCTTGC CTACATATCC 
CAGGTGCCCA CGCAAATGTC CTTCCTCTTC CGCCTCATCA ATATCATCCA TGTGCAGACG 
CTAACCCAGG AGAACGTCAG CTGCCTCAAC ACCAGCCTGG TAATCCTGAT GCTGGCCCGA 
CGGAAAGAGC GGCTGCCCCT GTACCTGCGA CTGCTACAGC GAATGGAGCA CAGCAAGAAA 
TACCCCGGCT TCCTGCTCAA CAACTTCCAC AACCTGCTGC GCTTTTGGCA GCAGCACTAC 
CTGCACAAGG ATAAGGACAG CACCTGCCTG GAGAATAGCT CCTGTATCAG CTTCTCGTAC 
TGGAAGGAGA CAGTGTCCAT CTTGTTGAAC CCAGACCGCC AGTCGCCCTC TGCCCTCGTC 
AGCTACATTG AGGAGCCCTA CATGGACATA GACAGAGACT TCACTGAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156924.1
CDS	79..2472
Translation	MAAAPAAAGAGASRGRRLAATAAAWGGWGGRPRPGNILLQLRQGQLTGRGLVRAVQFTETFLTERDKLSKWSGIPQLLLKLYATSHLHSDFVECQSILKEISPLLSMEAMAFVTEDRKFTQEATYPNTYIFDLFGGVDLLVEILMRPTISIRGQKLKISDEMSKDCLSILYNTCVCTEGVTKRLAEKNDFVIFLFTLMTSKKTFLQTATLIEDILGVKKEMIRLDEVPNLSSLVSNFDQQQLANFCRILAVTISEMDTGNDDKHTLLAKNAQQKKSLSLGPSAAEINQAALLSIPGFVERLCKLATRKVSESTGTASFLQELEEWYTWLDNALVLDALMRVANEESEHNQAPTVFPSLGTSEEGGLPHTSARAQLPQSMKIMHEIMYKLEVLYVLCVLLMGRQRNQVHRMIAEFKLIPGLNNLFDKLIWRKHSASALVLHGHNQNCDCSPDITLKIQFLRLLQSFSDHHENKYLLLNNQELNELSAISLKANIPEVEAVLNTDRSLVCDGKRGLLTRLLQVMKKEPAESSFRFWQARAVESFLRGTTSYADQMFLLKRGLLEHILYCIVDSECKSRDVLQSYFDLLGELMKFNVDAFKRFNKYINTDAKFQVFLKQINSSLVDSNMLVRCVTLSLDRFENQVDMKVAEVLSECRLLAYISQVPTQMSFLFRLINIIHVQTLTQENVSCLNTSLVILMLARRKERLPLYLRLLQRMEHSKKYPGFLLNNFHNLLRFWQQHYLHKDKDSTCLENSSCISFSYWKETVSILLNPDRQSPSALVSYIEEPYMDIDRDFTEE

Target ORF information:

RefSeq Version	NM_001163452.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily C, member 4 associated protein (Trpc4ap), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163452.1

ORF Insert Sequence:

ATGGCGGCAG CGCCCGCAGC GGCTGGAGCT GGGGCCAGCC GGGGGAGGCG GTTGGCAGCT 
ACCGCGGCGG CGTGGGGCGG CTGGGGAGGT CGACCGCGGC CGGGTAACAT CCTGCTGCAG 
CTGCGGCAGG GCCAGCTCAC CGGCCGAGGC CTGGTCCGGG CGGTGCAGTT CACTGAGACC 
TTTTTGACCG AGAGGGACAA ATTGTCCAAA TGGAGTGGAA TTCCTCAGTT GCTCCTTAAG 
CTGTATGCCA CCAGCCATCT GCACAGTGAC TTCGTTGAGT GCCAAAGCAT TCTCAAGGAG 
ATTTCTCCTC TTCTCTCTAT GGAGGCTATG GCGTTTGTAA CTGAAGACAG GAAATTTACT 
CAAGAAGCTA CATACCCAAA TACATATATT TTTGACTTAT TTGGAGGTGT TGATCTTCTT 
GTAGAAATTC TTATGAGGCC TACCATCTCT ATCCGGGGAC AGAAACTAAA AATAAGTGAC 
GAGATGTCGA AGGACTGCTT GAGCATCCTG TACAATACCT GTGTCTGCAC GGAAGGAGTA 
ACGAAGCGTT TGGCAGAAAA GAATGATTTT GTGATTTTTC TGTTCACACT GATGACAAGT 
AAGAAGACAT TCTTACAAAC AGCGACTCTC ATTGAAGACA TTTTGGGTGT TAAGAAGGAA 
ATGATCCGGC TAGATGAAGT TCCCAATCTG AGTTCCTTAG TATCCAATTT CGATCAGCAG 
CAGCTTGCTA ACTTCTGCAG GATCCTGGCT GTCACCATTT CAGAGATGGA CACGGGGAAT 
GACGACAAAC ACACGCTTCT TGCCAAAAAT GCTCAGCAGA AGAAGAGTTT GAGTTTGGGG 
CCTTCTGCAG CTGAAATCAA CCAAGCTGCT CTTCTCAGCA TCCCTGGCTT TGTGGAACGG 
CTTTGCAAAC TGGCAACTAG GAAAGTGTCA GAGTCAACGG GCACAGCCAG CTTCCTTCAA 
GAACTGGAGG AGTGGTACAC ATGGCTCGAC AATGCGTTGG TGCTAGATGC CCTGATGCGA 
GTGGCCAATG AGGAGTCTGA ACACAATCAA GCCCCCACTG TGTTCCCTTC TCTAGGCACT 
TCGGAGGAGG GTGGGCTGCC ACACACCTCG GCCAGGGCGC AGCTGCCCCA GTCCATGAAG 
ATTATGCATG AGATTATGTA CAAACTGGAA GTGCTTTATG TCCTCTGCGT GCTGCTGATG 
GGGCGTCAGC GAAACCAGGT CCACAGGATG ATTGCTGAGT TCAAGTTGAT CCCAGGACTC 
AATAATTTGT TCGACAAACT GATTTGGAGG AAGCATTCAG CATCTGCCCT TGTCCTCCAT 
GGCCACAACC AGAACTGTGA CTGTAGCCCG GACATCACCT TGAAGATCCA GTTTTTGAGA 
CTTCTCCAGA GTTTCAGTGA CCACCATGAG AACAAGTACT TGCTGCTCAA CAACCAGGAG 
CTAAATGAGC TCAGTGCCAT CTCGCTGAAA GCCAACATCC CTGAGGTGGA GGCTGTCCTC 
AACACTGACA GGAGTCTGGT GTGTGACGGC AAGAGGGGCC TACTAACTCG GCTGCTGCAG 
GTCATGAAGA AGGAGCCAGC GGAGTCTTCA TTCAGGTTTT GGCAAGCCCG GGCTGTGGAA 
AGTTTCCTCC GAGGGACCAC CTCCTATGCA GACCAGATGT TCCTGCTGAA GCGAGGCCTT 
CTGGAGCACA TCTTATACTG CATTGTGGAC AGTGAGTGCA AGTCTAGGGA TGTACTCCAG 
AGCTACTTTG ATCTCCTGGG AGAGCTGATG AAGTTCAACG TCGATGCATT CAAGAGATTC 
AATAAATATA TCAACACTGA TGCAAAGTTC CAGGTGTTCC TGAAACAGAT CAACAGCTCG 
CTAGTGGACT CCAACATGCT GGTGCGCTGT GTCACTCTGT CACTGGACCG ATTTGAAAAC 
CAGGTGGACA TGAAAGTGGC CGAGGTCCTG TCCGAGTGCC GCCTGCTTGC CTACATATCC 
CAGGTGCCCA CGCAAATGTC CTTCCTCTTC CGCCTCATCA ATATCATCCA TGTGCAGACG 
CTAACCCAGG AGAACGTCAG CTGCCTCAAC ACCAGCCTGG TAATCCTGAT GCTGGCCCGA 
CGGAAAGAGC GGCTGCCCCT GTACCTGCGA CTGCTACAGC GAATGGAGCA CAGCAAGAAA 
TACCCCGGCT TCCTGCTCAA CAACTTCCAC AACCTGCTGC GCTTTTGGCA GCAGCACTAC 
CTGCACAAGG ATAAGGACAG CACCTGCCTG GAGAATAGCT CCTGTATCAG CTTCTCGTAC 
TGGAAGGAGA CAGTGTCCAT CTTGTTGAAC CCAGACCGCC AGTCGCCCTC TGCCCTCGTC 
AGCTACATTG AGGAGCCCTA CATGGACATA GACAGAGACT TCACTGAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.
PLoS genetics10(10)e1004705(2014 Oct)
DiTommaso T,Jones LK,Cottle DL,,Gerdin AK,Vancollie VE,Watt FM,Ramirez-Solis R,Bradley A,Steel KP,Sundberg JP,White JK,Smyth IM

TRUSS, a novel tumor necrosis factor receptor 1 scaffolding protein that mediates activation of the transcription factor NF-kappaB.
Molecular and cellular biology23(22)8334-44(2003 Nov)
Soond SM,Terry JL,Colbert JD,Riches DW

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Trpc4ap cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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