The following Net1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Net1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu01148 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001047159.2 | |
| Accession Version | NM_001047159.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-05-13 | |
| Organism | Mus musculus(house mouse) | |
| Product | neuroepithelial cell-transforming gene 1 protein isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB598013.1, AK139921.1 and AK082128.1. On Sep 26, 2013 this sequence version replaced NM_001047159.1. Transcript Variant: This variant (2) lacks part of the 5' coding region, uses an alternate 5'-terminal exon, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK082128.1, BC004699.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGTGGCGC ATGATGAGAT CGGAGGTCTC CTGCCCATTA AAAGGACGAT CCGAGTCTTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001040624.1 |
| CDS | 197..1822 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001047159.2 |
1 | ATGGTGGCGC ATGATGAGAT CGGAGGTCTC CTGCCCATTA AAAGGACGAT CCGAGTCTTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu11355 | |
| Clone ID Related Accession (Same CDS sequence) | NM_019671.3 | |
| Accession Version | NM_019671.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-05-13 | |
| Organism | Mus musculus(house mouse) | |
| Product | neuroepithelial cell-transforming gene 1 protein isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139323.4, CF167482.1, AK082128.1 and AK139921.1. On Sep 26, 2013 this sequence version replaced NM_019671.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF094520.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAGCCAG AGCCGGCGGC TCAGAAGCAG CCCCGACCTC GGAGGAGAAG CCGCAGGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_062645.2 |
| CDS | 156..1943 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_019671.3 |
1 | ATGGAGCCAG AGCCGGCGGC TCAGAAGCAG CCCCGACCTC GGAGGAGAAG CCGCAGGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu86261 | |
| Clone ID Related Accession (Same CDS sequence) | XM_030247332.1 | |
| Accession Version | XM_030247332.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | neuroepithelial cell-transforming gene 1 protein isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTAGACA TCAATATGAA GGAGTCCTTG ACCACTCGAG AGATCAAACG TCAGGAGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_030103192.1 |
| CDS | 325..1638 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_030247332.1 |
1 | ATGCTAGACA TCAATATGAA GGAGTCCTTG ACCACTCGAG AGATCAAACG TCAGGAGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Acetylation of the RhoA GEF Net1A controls its subcellular localization and activity. |
The RhoGEF Net1 is required for normal mammary gland development. |
Rac1 controls the subcellular localization of the Rho guanine nucleotide exchange factor Net1A to regulate focal adhesion formation and cell spreading. |
Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation. |
