Mrpl12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mrpl12
Entrez Gene ID	56282
Full Name	mitochondrial ribosomal protein L12
Synonyms	0610034O11Rik,1500031N16Rik,MRP-L12,Rpml12
General protein information	Preferred Names mitochondrial ribosomal protein L12 Names 39S ribosomal protein L12, mitochondrial L12mt
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_027204.3	NP_081480.2	39S ribosomal protein L12, mitochondrial precursor
NM_027204.2	NP_081480.2	39S ribosomal protein L12, mitochondrial precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MRPL12	NP_963900.1
Mus musculus (house mouse)	Mrpl12	NP_081480.2
Neurospora crassa	NCU06469	XP_957360.1
Pan troglodytes (chimpanzee)	MRPL12	XP_001163363.1
Canis lupus familiaris (dog)	MRPL12	XP_850016.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011581	XP_309929.3
Gallus gallus (chicken)	MRPL12	XP_001232214.2
Drosophila melanogaster (fruit fly)	mRpL12	NP_524742.1
Caenorhabditis elegans (roundworm)	mrpl-12	NP_499360.1
Saccharomyces cerevisiae (baker's yeast)	MNP1	NP_011447.1
Kluyveromyces lactis	KLLA0D03212g	XP_453209.1
Magnaporthe oryzae (rice blast fungus)	MGG_06378	XP_003717196.1
Xenopus tropicalis (tropical clawed frog)	mrpl12	NP_001006886.1
Danio rerio (zebrafish)	mrpl12	NP_001017696.1
Schizosaccharomyces pombe (fission yeast)	SPCC4B3.09c	NP_588081.2
Homo sapiens (human)	MRPL12	NP_002940.2
	MRPL12	XP_001111712.1
Rattus norvegicus (Norway rat)	Mrpl12	NP_001025071.1
Eremothecium gossypii	AGOS_AFL089C	NP_985459.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import.
Nouws J, Goswami AV, Bestwick M, McCann BJ, Surovtseva YV, Shadel GS
The Journal of biological chemistry291(2)989-97(2016 Jan)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

Tagging genes with cassette-exchange sites.
Cobellis G, Nicolaus G, Iovino M, Romito A, Marra E, Barbarisi M, Sardiello M, Di Giorgio FP, Iovino N, Zollo M, Ballabio A, Cortese R
Nucleic acids research33(4)e44(2005 Mar)

Identification of mammalian mitochondrial ribosomal proteins (MRPs) by N-terminal sequencing of purified bovine MRPs and comparison to data bank sequences: the large subribosomal particle.
Graack HR, Bryant ML, O'Brien TW
Biochemistry38(50)16569-77(1999 Dec)

Exchangeable gene trap using the Cre/mutated lox system.
Araki K, Imaizumi T, Sekimoto T, Yoshinobu K, Yoshimuta J, Akizuki M, Miura K, Araki M, Yamamura K
Cellular and molecular biology (Noisy-le-Grand, France)45(5)737-50(1999 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

knock-down of MRPL12 by RNA interference results in instability of POLRMT.
Title: Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import.

The following Mrpl12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mrpl12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14588	NM_027204.3 Latest version!	Mus musculus mitochondrial ribosomal protein L12 (Mrpl12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
View Old Accession Versions >>
OMu14588	NM_027204.2	Mus musculus mitochondrial ribosomal protein L12 (Mrpl12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mrpl12 ( NM_027204.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081480.2 Mus musculus mitochondrial ribosomal protein L12 (Mrpl12), mRNA.

Price & Availability↑

CloneID	OMu14588
Clone ID Related Accession (Same CDS sequence)	NM_027204.3 , NM_027204.2
Accession Version	NM_027204.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	39S ribosomal protein L12, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK134816.1 and BQ032019.1. On Mar 1, 2018 this sequence version replaced NM_027204.2. ##Evidence-Data-START## Transcript exon combination :: BC019565.1, AK134816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGCTGCCGG TGGCGGCTAG CCGCTGCCTG TGGGGGCCAC GGCTTGGACT CCGGGGGGCA 
GCGCTTCGCC TTGCCAGGCA ACAGATGCCC AGCGTCTGTG CAGCGCGGCA GTTGAGAAGC 
AGCAGCCACC GAAGGAGTGA AGCACTCGCC GGCGCACCTC TGGATAATGC TCCCAAAGAA 
TACCCCCCCA AGATCCAGCA GCTGGTCCAA GACATTGCCA GCCTTACGCT CCTGGAGATC 
TCAGACCTCA ACGAACTCCT GAAGAAAACA TTGAAGATCC AGGATGTCGG CCTTATGCCA 
ATGGGTGGCA TGGTGCCTGG TCCCGTCTCT GCTGCAGCAC CGGCCTCTGA GGCGGCCGAG 
GAGGAAGACG TCCCCAAACA GAAAGAGCGG ACACACTTCA CCGTCCGCCT GACAGAGGCA 
AAGCCTGTGG ACAAAGTAAA GCTGATCAAG GAGATCAAGA ACTACGTCCA GGGCATCAAC 
CTCGTCCAGG CCAAGAAGCT GGTAGAGTCC CTGCCCCAGG AAATCAAAGC CAACGTCGCC 
AAAGCTGAGG CCGAGAAGAT CAAAGCAGCC TTGGAGGCAG TGGGTGGCAC CGTGGTTCTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081480.2
CDS	56..661
Translation	MLPVAASRCLWGPRLGLRGAALRLARQQMPSVCAARQLRSSSHRRSEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLMPMGGMVPGPVSAAAPASEAAEEEDVPKQKERTHFTVRLTEAKPVDKVKLIKEIKNYVQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE

Target ORF information:

RefSeq Version	NM_027204.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein L12 (Mrpl12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027204.3

ORF Insert Sequence:

ATGCTGCCGG TGGCGGCTAG CCGCTGCCTG TGGGGGCCAC GGCTTGGACT CCGGGGGGCA 
GCGCTTCGCC TTGCCAGGCA ACAGATGCCC AGCGTCTGTG CAGCGCGGCA GTTGAGAAGC 
AGCAGCCACC GAAGGAGTGA AGCACTCGCC GGCGCACCTC TGGATAATGC TCCCAAAGAA 
TACCCCCCCA AGATCCAGCA GCTGGTCCAA GACATTGCCA GCCTTACGCT CCTGGAGATC 
TCAGACCTCA ACGAACTCCT GAAGAAAACA TTGAAGATCC AGGATGTCGG CCTTATGCCA 
ATGGGTGGCA TGGTGCCTGG TCCCGTCTCT GCTGCAGCAC CGGCCTCTGA GGCGGCCGAG 
GAGGAAGACG TCCCCAAACA GAAAGAGCGG ACACACTTCA CCGTCCGCCT GACAGAGGCA 
AAGCCTGTGG ACAAAGTAAA GCTGATCAAG GAGATCAAGA ACTACGTCCA GGGCATCAAC 
CTCGTCCAGG CCAAGAAGCT GGTAGAGTCC CTGCCCCAGG AAATCAAAGC CAACGTCGCC 
AAAGCTGAGG CCGAGAAGAT CAAAGCAGCC TTGGAGGCAG TGGGTGGCAC CGTGGTTCTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mrpl12 ( NM_027204.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081480.2 Mus musculus mitochondrial ribosomal protein L12 (Mrpl12), mRNA.

Price & Availability↑

CloneID	OMu14588
Clone ID Related Accession (Same CDS sequence)	NM_027204.3 , NM_027204.2
Accession Version	NM_027204.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Mus musculus(house mouse)
Product	39S ribosomal protein L12, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC005712.1. On Aug 9, 2002 this sequence version replaced NM_027204.1. ##Evidence-Data-START## Transcript exon combination :: BC005712.1, AK134816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGCTGCCGG TGGCGGCTAG CCGCTGCCTG TGGGGGCCAC GGCTTGGACT CCGGGGGGCA 
GCGCTTCGCC TTGCCAGGCA ACAGATGCCC AGCGTCTGTG CAGCGCGGCA GTTGAGAAGC 
AGCAGCCACC GAAGGAGTGA AGCACTCGCC GGCGCACCTC TGGATAATGC TCCCAAAGAA 
TACCCCCCCA AGATCCAGCA GCTGGTCCAA GACATTGCCA GCCTTACGCT CCTGGAGATC 
TCAGACCTCA ACGAACTCCT GAAGAAAACA TTGAAGATCC AGGATGTCGG CCTTATGCCA 
ATGGGTGGCA TGGTGCCTGG TCCCGTCTCT GCTGCAGCAC CGGCCTCTGA GGCGGCCGAG 
GAGGAAGACG TCCCCAAACA GAAAGAGCGG ACACACTTCA CCGTCCGCCT GACAGAGGCA 
AAGCCTGTGG ACAAAGTAAA GCTGATCAAG GAGATCAAGA ACTACGTCCA GGGCATCAAC 
CTCGTCCAGG CCAAGAAGCT GGTAGAGTCC CTGCCCCAGG AAATCAAAGC CAACGTCGCC 
AAAGCTGAGG CCGAGAAGAT CAAAGCAGCC TTGGAGGCAG TGGGTGGCAC CGTGGTTCTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081480.2
CDS	24..629
Translation	MLPVAASRCLWGPRLGLRGAALRLARQQMPSVCAARQLRSSSHRRSEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLMPMGGMVPGPVSAAAPASEAAEEEDVPKQKERTHFTVRLTEAKPVDKVKLIKEIKNYVQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE

Target ORF information:

RefSeq Version	NM_027204.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein L12 (Mrpl12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027204.2

ORF Insert Sequence:

ATGCTGCCGG TGGCGGCTAG CCGCTGCCTG TGGGGGCCAC GGCTTGGACT CCGGGGGGCA 
GCGCTTCGCC TTGCCAGGCA ACAGATGCCC AGCGTCTGTG CAGCGCGGCA GTTGAGAAGC 
AGCAGCCACC GAAGGAGTGA AGCACTCGCC GGCGCACCTC TGGATAATGC TCCCAAAGAA 
TACCCCCCCA AGATCCAGCA GCTGGTCCAA GACATTGCCA GCCTTACGCT CCTGGAGATC 
TCAGACCTCA ACGAACTCCT GAAGAAAACA TTGAAGATCC AGGATGTCGG CCTTATGCCA 
ATGGGTGGCA TGGTGCCTGG TCCCGTCTCT GCTGCAGCAC CGGCCTCTGA GGCGGCCGAG 
GAGGAAGACG TCCCCAAACA GAAAGAGCGG ACACACTTCA CCGTCCGCCT GACAGAGGCA 
AAGCCTGTGG ACAAAGTAAA GCTGATCAAG GAGATCAAGA ACTACGTCCA GGGCATCAAC 
CTCGTCCAGG CCAAGAAGCT GGTAGAGTCC CTGCCCCAGG AAATCAAAGC CAACGTCGCC 
AAAGCTGAGG CCGAGAAGAT CAAAGCAGCC TTGGAGGCAG TGGGTGGCAC CGTGGTTCTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import.
The Journal of biological chemistry291(2)989-97(2016 Jan)
Nouws J,Goswami AV,Bestwick M,McCann BJ,Surovtseva YV,Shadel GS

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K

Tagging genes with cassette-exchange sites.
Nucleic acids research33(4)e44(2005 Mar)
Cobellis G,Nicolaus G,Iovino M,Romito A,Marra E,Barbarisi M,Sardiello M,Di Giorgio FP,Iovino N,Zollo M,Ballabio A,Cortese R

Identification of mammalian mitochondrial ribosomal proteins (MRPs) by N-terminal sequencing of purified bovine MRPs and comparison to data bank sequences: the large subribosomal particle.
Biochemistry38(50)16569-77(1999 Dec)
Graack HR,Bryant ML,O'Brien TW

Exchangeable gene trap using the Cre/mutated lox system.
Cellular and molecular biology (Noisy-le-Grand, France)45(5)737-50(1999 Jul)
Araki K,Imaizumi T,Sekimoto T,Yoshinobu K,Yoshimuta J,Akizuki M,Miura K,Araki M,Yamamura K

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Mrpl12 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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