gRNAs
The following Nphs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nphs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu20792 | |
| Clone ID Related Accession (Same CDS sequence) | NM_019459.2 | |
| Accession Version | NM_019459.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-25 | |
| Organism | Mus musculus(house mouse) | |
| Product | nephrin precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF191090.1, AF172256.1, AK141081.1 and AC167970.4. On Jan 4, 2007 this sequence version replaced NM_019459.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF172256.1, AF191090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN00849388 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGGAGCTA AGGAAGCCAC AGTCAGAGGC CCCGGGGCAA GCCCAGTGCA CAGAACTTGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_062332.2 |
| CDS | 87..3857 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_019459.2 |
1 | ATGGGAGCTA AGGAAGCCAC AGTCAGAGGC CCCGGGGCAA GCCCAGTGCA CAGAACTTGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu69564 | |
| Clone ID Related Accession (Same CDS sequence) | XM_011250647.2 , XM_011250647.1 | |
| Accession Version | XM_011250647.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | nephrin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250647.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAAAAAT GGAGAGCCTG GGATCCCCAG AGCATTCAGA GAAGGAAAAC AGCCAAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_011248949.1 |
| CDS | 257..3985 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_011250647.2 |
1 | ATGGAAAAAT GGAGAGCCTG GGATCCCCAG AGCATTCAGA GAAGGAAAAC AGCCAAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu69564 | |
| Clone ID Related Accession (Same CDS sequence) | XM_011250647.2 , XM_011250647.1 | |
| Accession Version | XM_011250647.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | nephrin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAAAAAT GGAGAGCCTG GGATCCCCAG AGCATTCAGA GAAGGAAAAC AGCCAAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_011248949.1 |
| CDS | 258..3986 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_011250647.1 |
1 | ATGGAAAAAT GGAGAGCCTG GGATCCCCAG AGCATTCAGA GAAGGAAAAC AGCCAAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The WD40-domain containing protein CORO2B is specifically enriched in glomerular podocytes and regulates the ventral actin cytoskeleton. |
