Pde3a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde3a
Entrez Gene ID	54611
Full Name	phosphodiesterase 3A, cGMP inhibited
Synonyms	A930022O17Rik,C87899
General protein information	Preferred Names phosphodiesterase 3A, cGMP inhibited Names cGMP-inhibited 3',5'-cyclic phosphodiesterase A CGI-PDE A cGMP-inhibited phosphodiesterase cyclic GMP-inhibited phosphodiesterase A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 G2

mRNA and Protein(s)

mRNA	Protein	Name
XM_011241595.3	XP_011239897.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X1
NM_018779.1	NP_061249.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A
XM_006506979.3	XP_006507042.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X2
NM_018779.2	NP_061249.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A
XM_011241595.2	XP_011239897.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X1
XM_006506979.4	XP_006507042.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X2

Pathways from BioSystems

Homologies

	PDE3A	XP_001096963.2
Canis lupus familiaris (dog)	PDE3A	XP_005637137.1
Rattus norvegicus (Norway rat)	Pde3a	NP_059033.1
Danio rerio (zebrafish)	pde3a	XP_695432.4
Pan troglodytes (chimpanzee)	PDE3A	XP_520783.2
Mus musculus (house mouse)	Pde3a	NP_061249.1
Gallus gallus (chicken)	PDE3A	XP_416416.4
Homo sapiens (human)	PDE3A	NP_000912.3
Bos taurus (cattle)	PDE3A	XP_002687798.1

Gene Ontology
Bibliography

Related articles in PubMed

Phosphodiesterase 3A expression and activity in the murine vasculature is influenced by NO-sensitive guanylyl cyclase.
D?nnes S, Voussen B, Aue A, Groneberg K, Nikolaev V, Groneberg D, Friebe A
Pflugers Archiv : European journal of physiology(2018 Jan)

Phosphodiesterase 3A: a new player in development of interstitial cells of Cajal and a prospective target in gastrointestinal stromal tumors (GIST).
Vandenberghe P, Hagu? P, Hockman SC, Manganiello VC, Demetter P, Erneux C, Vanderwinden JM
Oncotarget8(25)41026-41043(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PDE3A is a low-affinity and high-velocity enzyme for hydrolysis of cUMP in cardiac myocytes.
Title: cUMP hydrolysis by PDE3A.

These together indicate that PDE3A1 negatively regulates beta-AR signaling and protects against I/R injury by inhibiting cardiomyocyte apoptosis.
Title: Cyclic nucleotide phosphodiesterase 3A1 protects the heart against ischemia-reperfusion injury.

PDE3A is the primary isozyme modulating basal myocardial contractility and sarcoplasmic reticulum divalent calcium ion (Ca2+) content.
Title: Phosphodiesterase type 3A regulates basal myocardial contractility through interacting with sarcoplasmic reticulum calcium ATPase type 2a signaling complexes in mouse heart.

Discussion of role of PDE3A, not PDE3B, in cell cycle progression in vascular smooth muscle and oocytes via regulation of cAMP levels. Is inhibition of PDE3A a possible target for treatment of cardiovascular diseases or female infertility? [REVIEW]
Title: Role of PDE3A in regulation of cell cycle progression in mouse vascular smooth muscle cells and oocytes: implications in cardiovascular diseases and infertility.

PDE3A regulates VSMC growth via two complementary pathways
Title: Phosphodiesterase 3A (PDE3A) deletion suppresses proliferation of cultured murine vascular smooth muscle cells (VSMCs) via inhibition of mitogen-activated protein kinase (MAPK) signaling and alterations in critical cell cycle regulatory proteins.

The following Pde3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu37834	XM_006506979.4 Latest version!	Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu69560	XM_011241595.3 Latest version!	Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu10996	NM_018779.2 Latest version!	Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu10996	NM_018779.1	Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu69560	XM_011241595.2	Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu37834	XM_006506979.3	Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde3a ( XM_006506979.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507042.1 Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu37834
Clone ID Related Accession (Same CDS sequence)	XM_006506979.3 , XM_006506979.4
Accession Version	XM_006506979.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506979.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACC AGTCGTCTGG GATGATAGGC ACTTCAGTGC AGACATATCC CAAGCTCATG 
GGCCATTCAG AATGGGACCA CAAGAGAGGG CCACGAGGAT CCCAGTCAGG AACCAGCATC 
ACTGTGGATA TTGCTGTGAT GGGTGAGGCC CATGGCCTCA TTACCGACCT CCTGGCCGAC 
CCTTCTCTGC CCCCGAATGT GTGTACATCC TTGAGGGCCG TAAGCAACTT GCTCAGCACC 
CAGCTCACCT TCCAGGCCAT CCACAAGCCC AGAGTGAACC CCACCGTGAC CTTCAGTGAG 
AACTATACCT GCTCGGACTC TGAGGAAGGC TTGGAGAAAG ACAAGCAGGC CATTTCCAAG 
CGCCTGAGAA GAAGTTTGCC TCCAGGCTTA CTGAGAAGAG TCTCGTCAAC TTGGACAACT 
ACCACCTCTG CCACAGGTCT GCCCACCTTG GAGCCTGCGC CAGTGCGGAG GGACCGCAGT 
GCCAGCATAA AGCCGCACGA AGCCCCTTCA CCCAGTGCTG TCAATCCTGA CTCTTGGAAT 
GCCCCAGGGT TGACGACTCT CACCAAAAGC AGATCCTTCA CTTCATCCTA TGCTGTCTCT 
GCAGCTAACC ATGTAAAAGC TAAAAAGCAA AACAGGCCAG GTGGCCTTGC TAAAATTTCC 
CCCGTCCCCT CACCCTCCTC CTCGCCTCCC CAGGGATCAC CTGCCAGCAG TCCTGTCAGC 
AACAGTGCTT CACAACAGTT CCCAGAATCC CCTGAAGTAA CCATCAAGCG AGGGCCTGGA 
TCTCACAGGG CCTTAACTTA CACCCAAAGT GCCCCTGACC TGTCCCCTCA GATCCCACCC 
CCGTCTGTCA TATGTAGCAG CTGTGGCAGA CCTTATTCGC AAGGGAATCC TGCTGATGGA 
CCCTCAGAGA GAAGTGGCCC AGCCATGCTG AAACCAAACA GAACAGATGA CACTTCTCAA 
GTTACCTCCG ATTATGAGAC CAACAACAAC AGTGACAGCA GTGACATTCT GCAGAATGAA 
GAGGAAGCTG AGTGCCAGAG AGAACCCCAG AGGAAAGCAT CGGCTTGTGG CACCTATACT 
TCCCAGACCA TGATCTTCCT GGACAAACCA ATTCTTGCTC CAGAACCCCT GGTCATGGAT 
AACCTGGACT CAATTATGGA TCAGTTGAAC ACCTGGAATT TTCCAATTTT TGATTTAATG 
GAAAATATAG GAAGAAAATG TGGCCGTATT CTGAGCCAGG TATCATACAG ACTGTTTGAA 
GACATGGGGC TCTTTGAAGC CTTTAAAATC CCGGTTAGGG AGTTTATGAA TTACTTTCAT 
GCTTTGGAGA TCGGATACAG GGACATTCCT TATCATAACA GAATCCATGC CACCGATGTT 
CTACACGCTG TGTGGTATCT CACAACACAG CCGATTCCTG GCCTCCCAAG TGTGATTGGT 
GATCACGGCT CAGCAAGCGA CTCCGATTCT GACAGTGGAT TTACACACGG ACACATGGGA 
TATGTGTTTT CAAAAATGTA TCACGTGCCA GATGACAAAT ATGGATGCCT GTCTGGGAAT 
ATTCCAGCCC TAGAGTTGAT GGCTCTGTAC GTTGCTGCAG CCATGCATGA CTACGATCAC 
CCAGGGAGGA CAAATGCCTT CCTGGTTGCC ACTAGTGCCC CTCAGGCGGT GCTATACAAC 
GACCGTTCTG TTCTGGAGAA CCATCACGCC GCTGCAGCCT GGAATCTCTT CATGTCCCGG 
CCAGAGTATA ACTTCTTAGT TAACCTGGAC CACGTGGAAT TCAAGCACTT CCGCTTCCTA 
GTCATTGAAG CGATTTTGGC TACTGACCTG AAGAAACACT TTGACTTTGT AGCCAAATTT 
AACGCCAAGG TAAACGATGA TGTCGGGATA GATTGGACCA ATGAAAACGA CCGTCTCCTG 
GTGTGTCAGA TGTGTATAAA GCTGGCTGAC ATCAACGGGC CTGCTAAATG CAAAGAACTC 
CACCTGCGGT GGACAGAGGG CATCGCCAGC GAGTTTTATG AGCAGGGCGA TGAAGAAGCC 
AGCCTCGGCT TGCCCATAAG TCCCTTTATG GACCGCTCTG CCCCGCAGCT GGCCAATCTT 
CAGGAATCCT TTATCTCACA CATCGTGGGT CCTCTCTGCC ACTCCTATGA CTCTGCGGGA 
CTCATGCCAG GAAAATGGGT AGATGACAGT GATGATTCCG GAGACACCGA TGACCCGGAA 
GAAGAGGAGG AAGAGGCTGA AACACCACAC GAGGACGAAG CCTGTGAAAG CAGTATAGCT 
CCCCGAAAGA AAAGTTTCAA AAGAAGGAGA ATCTACTGCC AAATAACTCA GCACCTTCTG 
CAGAACCACA TGATGTGGAA GAAAGTGATT GAAGAGGAAC AGTGTTTATC AGGCACAGAG 
AACCAGTCCC TGGACCAGGT CCCTCTGCAG CATCCCTCAG AGCAGATCCA GGCTATCAAG 
GAGGAAGAGG AAGAGAAGGG GAAGCCAAGA GCAGAGGAGA CCCTGGCTCC ACAGCCAGAC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507042.1
CDS	1513..4038
Translation	MQDQSSGMIGTSVQTYPKLMGHSEWDHKRGPRGSQSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPTVTFSENYTCSDSEEGLEKDKQAISKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSASIKPHEAPSPSAVNPDSWNAPGLTTLTKSRSFTSSYAVSAANHVKAKKQNRPGGLAKISPVPSPSSSPPQGSPASSPVSNSASQQFPESPEVTIKRGPGSHRALTYTQSAPDLSPQIPPPSVICSSCGRPYSQGNPADGPSERSGPAMLKPNRTDDTSQVTSDYETNNNSDSSDILQNEEEAECQREPQRKASACGTYTSQTMIFLDKPILAPEPLVMDNLDSIMDQLNTWNFPIFDLMENIGRKCGRILSQVSYRLFEDMGLFEAFKIPVREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLPSVIGDHGSASDSDSDSGFTHGHMGYVFSKMYHVPDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLVNLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNAKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLRWTEGIASEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCHSYDSAGLMPGKWVDDSDDSGDTDDPEEEEEEAETPHEDEACESSIAPRKKSFKRRRIYCQITQHLLQNHMMWKKVIEEEQCLSGTENQSLDQVPLQHPSEQIQAIKEEEEEKGKPRAEETLAPQPDL

Target ORF information:

RefSeq Version	XM_006506979.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506979.3

ORF Insert Sequence:

ATGCAGGACC AGTCGTCTGG GATGATAGGC ACTTCAGTGC AGACATATCC CAAGCTCATG 
GGCCATTCAG AATGGGACCA CAAGAGAGGG CCACGAGGAT CCCAGTCAGG AACCAGCATC 
ACTGTGGATA TTGCTGTGAT GGGTGAGGCC CATGGCCTCA TTACCGACCT CCTGGCCGAC 
CCTTCTCTGC CCCCGAATGT GTGTACATCC TTGAGGGCCG TAAGCAACTT GCTCAGCACC 
CAGCTCACCT TCCAGGCCAT CCACAAGCCC AGAGTGAACC CCACCGTGAC CTTCAGTGAG 
AACTATACCT GCTCGGACTC TGAGGAAGGC TTGGAGAAAG ACAAGCAGGC CATTTCCAAG 
CGCCTGAGAA GAAGTTTGCC TCCAGGCTTA CTGAGAAGAG TCTCGTCAAC TTGGACAACT 
ACCACCTCTG CCACAGGTCT GCCCACCTTG GAGCCTGCGC CAGTGCGGAG GGACCGCAGT 
GCCAGCATAA AGCCGCACGA AGCCCCTTCA CCCAGTGCTG TCAATCCTGA CTCTTGGAAT 
GCCCCAGGGT TGACGACTCT CACCAAAAGC AGATCCTTCA CTTCATCCTA TGCTGTCTCT 
GCAGCTAACC ATGTAAAAGC TAAAAAGCAA AACAGGCCAG GTGGCCTTGC TAAAATTTCC 
CCCGTCCCCT CACCCTCCTC CTCGCCTCCC CAGGGATCAC CTGCCAGCAG TCCTGTCAGC 
AACAGTGCTT CACAACAGTT CCCAGAATCC CCTGAAGTAA CCATCAAGCG AGGGCCTGGA 
TCTCACAGGG CCTTAACTTA CACCCAAAGT GCCCCTGACC TGTCCCCTCA GATCCCACCC 
CCGTCTGTCA TATGTAGCAG CTGTGGCAGA CCTTATTCGC AAGGGAATCC TGCTGATGGA 
CCCTCAGAGA GAAGTGGCCC AGCCATGCTG AAACCAAACA GAACAGATGA CACTTCTCAA 
GTTACCTCCG ATTATGAGAC CAACAACAAC AGTGACAGCA GTGACATTCT GCAGAATGAA 
GAGGAAGCTG AGTGCCAGAG AGAACCCCAG AGGAAAGCAT CGGCTTGTGG CACCTATACT 
TCCCAGACCA TGATCTTCCT GGACAAACCA ATTCTTGCTC CAGAACCCCT GGTCATGGAT 
AACCTGGACT CAATTATGGA TCAGTTGAAC ACCTGGAATT TTCCAATTTT TGATTTAATG 
GAAAATATAG GAAGAAAATG TGGCCGTATT CTGAGCCAGG TATCATACAG ACTGTTTGAA 
GACATGGGGC TCTTTGAAGC CTTTAAAATC CCGGTTAGGG AGTTTATGAA TTACTTTCAT 
GCTTTGGAGA TCGGATACAG GGACATTCCT TATCATAACA GAATCCATGC CACCGATGTT 
CTACACGCTG TGTGGTATCT CACAACACAG CCGATTCCTG GCCTCCCAAG TGTGATTGGT 
GATCACGGCT CAGCAAGCGA CTCCGATTCT GACAGTGGAT TTACACACGG ACACATGGGA 
TATGTGTTTT CAAAAATGTA TCACGTGCCA GATGACAAAT ATGGATGCCT GTCTGGGAAT 
ATTCCAGCCC TAGAGTTGAT GGCTCTGTAC GTTGCTGCAG CCATGCATGA CTACGATCAC 
CCAGGGAGGA CAAATGCCTT CCTGGTTGCC ACTAGTGCCC CTCAGGCGGT GCTATACAAC 
GACCGTTCTG TTCTGGAGAA CCATCACGCC GCTGCAGCCT GGAATCTCTT CATGTCCCGG 
CCAGAGTATA ACTTCTTAGT TAACCTGGAC CACGTGGAAT TCAAGCACTT CCGCTTCCTA 
GTCATTGAAG CGATTTTGGC TACTGACCTG AAGAAACACT TTGACTTTGT AGCCAAATTT 
AACGCCAAGG TAAACGATGA TGTCGGGATA GATTGGACCA ATGAAAACGA CCGTCTCCTG 
GTGTGTCAGA TGTGTATAAA GCTGGCTGAC ATCAACGGGC CTGCTAAATG CAAAGAACTC 
CACCTGCGGT GGACAGAGGG CATCGCCAGC GAGTTTTATG AGCAGGGCGA TGAAGAAGCC 
AGCCTCGGCT TGCCCATAAG TCCCTTTATG GACCGCTCTG CCCCGCAGCT GGCCAATCTT 
CAGGAATCCT TTATCTCACA CATCGTGGGT CCTCTCTGCC ACTCCTATGA CTCTGCGGGA 
CTCATGCCAG GAAAATGGGT AGATGACAGT GATGATTCCG GAGACACCGA TGACCCGGAA 
GAAGAGGAGG AAGAGGCTGA AACACCACAC GAGGACGAAG CCTGTGAAAG CAGTATAGCT 
CCCCGAAAGA AAAGTTTCAA AAGAAGGAGA ATCTACTGCC AAATAACTCA GCACCTTCTG 
CAGAACCACA TGATGTGGAA GAAAGTGATT GAAGAGGAAC AGTGTTTATC AGGCACAGAG 
AACCAGTCCC TGGACCAGGT CCCTCTGCAG CATCCCTCAG AGCAGATCCA GGCTATCAAG 
GAGGAAGAGG AAGAGAAGGG GAAGCCAAGA GCAGAGGAGA CCCTGGCTCC ACAGCCAGAC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde3a ( XM_011241595.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239897.1 Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69560
Clone ID Related Accession (Same CDS sequence)	XM_011241595.3 , XM_011241595.2
Accession Version	XM_011241595.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCCG TGCTGGCTCA GCATCTACTT GGTGCCATTT GTTTCTTCAG TGGCAGCTAT 
CTGACTCAGC TCATGGGCCA TTCAGAATGG GACCACAAGA GAGGGCCACG AGGATCCCAG 
TCAGGAACCA GCATCACTGT GGATATTGCT GTGATGGGTG AGGCCCATGG CCTCATTACC 
GACCTCCTGG CCGACCCTTC TCTGCCCCCG AATGTGTGTA CATCCTTGAG GGCCGTAAGC 
AACTTGCTCA GCACCCAGCT CACCTTCCAG GCCATCCACA AGCCCAGAGT GAACCCCACC 
GTGACCTTCA GTGAGAACTA TACCTGCTCG GACTCTGAGG AAGGCTTGGA GAAAGACAAG 
CAGGCCATTT CCAAGCGCCT GAGAAGAAGT TTGCCTCCAG GCTTACTGAG AAGAGTCTCG 
TCAACTTGGA CAACTACCAC CTCTGCCACA GGTCTGCCCA CCTTGGAGCC TGCGCCAGTG 
CGGAGGGACC GCAGTGCCAG CATAAAGCCG CACGAAGCCC CTTCACCCAG TGCTGTCAAT 
CCTGACTCTT GGAATGCCCC AGGGTTGACG ACTCTCACCA AAAGCAGATC CTTCACTTCA 
TCCTATGCTG TCTCTGCAGC TAACCATGTA AAAGCTAAAA AGCAAAACAG GCCAGGTGGC 
CTTGCTAAAA TTTCCCCCGT CCCCTCACCC TCCTCCTCGC CTCCCCAGGG ATCACCTGCC 
AGCAGTCCTG TCAGCAACAG TGCTTCACAA CAGTTCCCAG AATCCCCTGA AGTAACCATC 
AAGCGAGGGC CTGGATCTCA CAGGGCCTTA ACTTACACCC AAAGTGCCCC TGACCTGTCC 
CCTCAGATCC CACCCCCGTC TGTCATATGT AGCAGCTGTG GCAGACCTTA TTCGCAAGGG 
AATCCTGCTG ATGGACCCTC AGAGAGAAGT GGCCCAGCCA TGCTGAAACC AAACAGAACA 
GATGACACTT CTCAAGTTAC CTCCGATTAT GAGACCAACA ACAACAGTGA CAGCAGTGAC 
ATTCTGCAGA ATGAAGAGGA AGCTGAGTGC CAGAGAGAAC CCCAGAGGAA AGCATCGGCT 
TGTGGCACCT ATACTTCCCA GACCATGATC TTCCTGGACA AACCAATTCT TGCTCCAGAA 
CCCCTGGTCA TGGATAACCT GGACTCAATT ATGGATCAGT TGAACACCTG GAATTTTCCA 
ATTTTTGATT TAATGGAAAA TATAGGAAGA AAATGTGGCC GTATTCTGAG CCAGGTATCA 
TACAGACTGT TTGAAGACAT GGGGCTCTTT GAAGCCTTTA AAATCCCGGT TAGGGAGTTT 
ATGAATTACT TTCATGCTTT GGAGATCGGA TACAGGGACA TTCCTTATCA TAACAGAATC 
CATGCCACCG ATGTTCTACA CGCTGTGTGG TATCTCACAA CACAGCCGAT TCCTGGCCTC 
CCAAGTGTGA TTGGTGATCA CGGCTCAGCA AGCGACTCCG ATTCTGACAG TGGATTTACA 
CACGGACACA TGGGATATGT GTTTTCAAAA ATGTATCACG TGCCAGATGA CAAATATGGA 
TGCCTGTCTG GGAATATTCC AGCCCTAGAG TTGATGGCTC TGTACGTTGC TGCAGCCATG 
CATGACTACG ATCACCCAGG GAGGACAAAT GCCTTCCTGG TTGCCACTAG TGCCCCTCAG 
GCGGTGCTAT ACAACGACCG TTCTGTTCTG GAGAACCATC ACGCCGCTGC AGCCTGGAAT 
CTCTTCATGT CCCGGCCAGA GTATAACTTC TTAGTTAACC TGGACCACGT GGAATTCAAG 
CACTTCCGCT TCCTAGTCAT TGAAGCGATT TTGGCTACTG ACCTGAAGAA ACACTTTGAC 
TTTGTAGCCA AATTTAACGC CAAGGTAAAC GATGATGTCG GGATAGATTG GACCAATGAA 
AACGACCGTC TCCTGGTGTG TCAGATGTGT ATAAAGCTGG CTGACATCAA CGGGCCTGCT 
AAATGCAAAG AACTCCACCT GCGGTGGACA GAGGGCATCG CCAGCGAGTT TTATGAGCAG 
GGCGATGAAG AAGCCAGCCT CGGCTTGCCC ATAAGTCCCT TTATGGACCG CTCTGCCCCG 
CAGCTGGCCA ATCTTCAGGA ATCCTTTATC TCACACATCG TGGGTCCTCT CTGCCACTCC 
TATGACTCTG CGGGACTCAT GCCAGGAAAA TGGGTAGATG ACAGTGATGA TTCCGGAGAC 
ACCGATGACC CGGAAGAAGA GGAGGAAGAG GCTGAAACAC CACACGAGGA CGAAGCCTGT 
GAAAGCAGTA TAGCTCCCCG AAAGAAAAGT TTCAAAAGAA GGAGAATCTA CTGCCAAATA 
ACTCAGCACC TTCTGCAGAA CCACATGATG TGGAAGAAAG TGATTGAAGA GGAACAGTGT 
TTATCAGGCA CAGAGAACCA GTCCCTGGAC CAGGTCCCTC TGCAGCATCC CTCAGAGCAG 
ATCCAGGCTA TCAAGGAGGA AGAGGAAGAG AAGGGGAAGC CAAGAGCAGA GGAGACCCTG 
GCTCCACAGC CAGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239897.1
CDS	379..2919
Translation	MKAVLAQHLLGAICFFSGSYLTQLMGHSEWDHKRGPRGSQSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPTVTFSENYTCSDSEEGLEKDKQAISKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSASIKPHEAPSPSAVNPDSWNAPGLTTLTKSRSFTSSYAVSAANHVKAKKQNRPGGLAKISPVPSPSSSPPQGSPASSPVSNSASQQFPESPEVTIKRGPGSHRALTYTQSAPDLSPQIPPPSVICSSCGRPYSQGNPADGPSERSGPAMLKPNRTDDTSQVTSDYETNNNSDSSDILQNEEEAECQREPQRKASACGTYTSQTMIFLDKPILAPEPLVMDNLDSIMDQLNTWNFPIFDLMENIGRKCGRILSQVSYRLFEDMGLFEAFKIPVREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLPSVIGDHGSASDSDSDSGFTHGHMGYVFSKMYHVPDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLVNLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNAKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLRWTEGIASEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCHSYDSAGLMPGKWVDDSDDSGDTDDPEEEEEEAETPHEDEACESSIAPRKKSFKRRRIYCQITQHLLQNHMMWKKVIEEEQCLSGTENQSLDQVPLQHPSEQIQAIKEEEEEKGKPRAEETLAPQPDL

Target ORF information:

RefSeq Version	XM_011241595.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241595.2

ORF Insert Sequence:

ATGAAGGCCG TGCTGGCTCA GCATCTACTT GGTGCCATTT GTTTCTTCAG TGGCAGCTAT 
CTGACTCAGC TCATGGGCCA TTCAGAATGG GACCACAAGA GAGGGCCACG AGGATCCCAG 
TCAGGAACCA GCATCACTGT GGATATTGCT GTGATGGGTG AGGCCCATGG CCTCATTACC 
GACCTCCTGG CCGACCCTTC TCTGCCCCCG AATGTGTGTA CATCCTTGAG GGCCGTAAGC 
AACTTGCTCA GCACCCAGCT CACCTTCCAG GCCATCCACA AGCCCAGAGT GAACCCCACC 
GTGACCTTCA GTGAGAACTA TACCTGCTCG GACTCTGAGG AAGGCTTGGA GAAAGACAAG 
CAGGCCATTT CCAAGCGCCT GAGAAGAAGT TTGCCTCCAG GCTTACTGAG AAGAGTCTCG 
TCAACTTGGA CAACTACCAC CTCTGCCACA GGTCTGCCCA CCTTGGAGCC TGCGCCAGTG 
CGGAGGGACC GCAGTGCCAG CATAAAGCCG CACGAAGCCC CTTCACCCAG TGCTGTCAAT 
CCTGACTCTT GGAATGCCCC AGGGTTGACG ACTCTCACCA AAAGCAGATC CTTCACTTCA 
TCCTATGCTG TCTCTGCAGC TAACCATGTA AAAGCTAAAA AGCAAAACAG GCCAGGTGGC 
CTTGCTAAAA TTTCCCCCGT CCCCTCACCC TCCTCCTCGC CTCCCCAGGG ATCACCTGCC 
AGCAGTCCTG TCAGCAACAG TGCTTCACAA CAGTTCCCAG AATCCCCTGA AGTAACCATC 
AAGCGAGGGC CTGGATCTCA CAGGGCCTTA ACTTACACCC AAAGTGCCCC TGACCTGTCC 
CCTCAGATCC CACCCCCGTC TGTCATATGT AGCAGCTGTG GCAGACCTTA TTCGCAAGGG 
AATCCTGCTG ATGGACCCTC AGAGAGAAGT GGCCCAGCCA TGCTGAAACC AAACAGAACA 
GATGACACTT CTCAAGTTAC CTCCGATTAT GAGACCAACA ACAACAGTGA CAGCAGTGAC 
ATTCTGCAGA ATGAAGAGGA AGCTGAGTGC CAGAGAGAAC CCCAGAGGAA AGCATCGGCT 
TGTGGCACCT ATACTTCCCA GACCATGATC TTCCTGGACA AACCAATTCT TGCTCCAGAA 
CCCCTGGTCA TGGATAACCT GGACTCAATT ATGGATCAGT TGAACACCTG GAATTTTCCA 
ATTTTTGATT TAATGGAAAA TATAGGAAGA AAATGTGGCC GTATTCTGAG CCAGGTATCA 
TACAGACTGT TTGAAGACAT GGGGCTCTTT GAAGCCTTTA AAATCCCGGT TAGGGAGTTT 
ATGAATTACT TTCATGCTTT GGAGATCGGA TACAGGGACA TTCCTTATCA TAACAGAATC 
CATGCCACCG ATGTTCTACA CGCTGTGTGG TATCTCACAA CACAGCCGAT TCCTGGCCTC 
CCAAGTGTGA TTGGTGATCA CGGCTCAGCA AGCGACTCCG ATTCTGACAG TGGATTTACA 
CACGGACACA TGGGATATGT GTTTTCAAAA ATGTATCACG TGCCAGATGA CAAATATGGA 
TGCCTGTCTG GGAATATTCC AGCCCTAGAG TTGATGGCTC TGTACGTTGC TGCAGCCATG 
CATGACTACG ATCACCCAGG GAGGACAAAT GCCTTCCTGG TTGCCACTAG TGCCCCTCAG 
GCGGTGCTAT ACAACGACCG TTCTGTTCTG GAGAACCATC ACGCCGCTGC AGCCTGGAAT 
CTCTTCATGT CCCGGCCAGA GTATAACTTC TTAGTTAACC TGGACCACGT GGAATTCAAG 
CACTTCCGCT TCCTAGTCAT TGAAGCGATT TTGGCTACTG ACCTGAAGAA ACACTTTGAC 
TTTGTAGCCA AATTTAACGC CAAGGTAAAC GATGATGTCG GGATAGATTG GACCAATGAA 
AACGACCGTC TCCTGGTGTG TCAGATGTGT ATAAAGCTGG CTGACATCAA CGGGCCTGCT 
AAATGCAAAG AACTCCACCT GCGGTGGACA GAGGGCATCG CCAGCGAGTT TTATGAGCAG 
GGCGATGAAG AAGCCAGCCT CGGCTTGCCC ATAAGTCCCT TTATGGACCG CTCTGCCCCG 
CAGCTGGCCA ATCTTCAGGA ATCCTTTATC TCACACATCG TGGGTCCTCT CTGCCACTCC 
TATGACTCTG CGGGACTCAT GCCAGGAAAA TGGGTAGATG ACAGTGATGA TTCCGGAGAC 
ACCGATGACC CGGAAGAAGA GGAGGAAGAG GCTGAAACAC CACACGAGGA CGAAGCCTGT 
GAAAGCAGTA TAGCTCCCCG AAAGAAAAGT TTCAAAAGAA GGAGAATCTA CTGCCAAATA 
ACTCAGCACC TTCTGCAGAA CCACATGATG TGGAAGAAAG TGATTGAAGA GGAACAGTGT 
TTATCAGGCA CAGAGAACCA GTCCCTGGAC CAGGTCCCTC TGCAGCATCC CTCAGAGCAG 
ATCCAGGCTA TCAAGGAGGA AGAGGAAGAG AAGGGGAAGC CAAGAGCAGA GGAGACCCTG 
GCTCCACAGC CAGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde3a ( XM_006506979.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507042.1 Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), transcript variant X2, mRNA.

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CloneID	OMu37834
Clone ID Related Accession (Same CDS sequence)	XM_006506979.3 , XM_006506979.4
Accession Version	XM_006506979.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506979.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACC AGTCGTCTGG GATGATAGGC ACTTCAGTGC AGACATATCC CAAGCTCATG 
GGCCATTCAG AATGGGACCA CAAGAGAGGG CCACGAGGAT CCCAGTCAGG AACCAGCATC 
ACTGTGGATA TTGCTGTGAT GGGTGAGGCC CATGGCCTCA TTACCGACCT CCTGGCCGAC 
CCTTCTCTGC CCCCGAATGT GTGTACATCC TTGAGGGCCG TAAGCAACTT GCTCAGCACC 
CAGCTCACCT TCCAGGCCAT CCACAAGCCC AGAGTGAACC CCACCGTGAC CTTCAGTGAG 
AACTATACCT GCTCGGACTC TGAGGAAGGC TTGGAGAAAG ACAAGCAGGC CATTTCCAAG 
CGCCTGAGAA GAAGTTTGCC TCCAGGCTTA CTGAGAAGAG TCTCGTCAAC TTGGACAACT 
ACCACCTCTG CCACAGGTCT GCCCACCTTG GAGCCTGCGC CAGTGCGGAG GGACCGCAGT 
GCCAGCATAA AGCCGCACGA AGCCCCTTCA CCCAGTGCTG TCAATCCTGA CTCTTGGAAT 
GCCCCAGGGT TGACGACTCT CACCAAAAGC AGATCCTTCA CTTCATCCTA TGCTGTCTCT 
GCAGCTAACC ATGTAAAAGC TAAAAAGCAA AACAGGCCAG GTGGCCTTGC TAAAATTTCC 
CCCGTCCCCT CACCCTCCTC CTCGCCTCCC CAGGGATCAC CTGCCAGCAG TCCTGTCAGC 
AACAGTGCTT CACAACAGTT CCCAGAATCC CCTGAAGTAA CCATCAAGCG AGGGCCTGGA 
TCTCACAGGG CCTTAACTTA CACCCAAAGT GCCCCTGACC TGTCCCCTCA GATCCCACCC 
CCGTCTGTCA TATGTAGCAG CTGTGGCAGA CCTTATTCGC AAGGGAATCC TGCTGATGGA 
CCCTCAGAGA GAAGTGGCCC AGCCATGCTG AAACCAAACA GAACAGATGA CACTTCTCAA 
GTTACCTCCG ATTATGAGAC CAACAACAAC AGTGACAGCA GTGACATTCT GCAGAATGAA 
GAGGAAGCTG AGTGCCAGAG AGAACCCCAG AGGAAAGCAT CGGCTTGTGG CACCTATACT 
TCCCAGACCA TGATCTTCCT GGACAAACCA ATTCTTGCTC CAGAACCCCT GGTCATGGAT 
AACCTGGACT CAATTATGGA TCAGTTGAAC ACCTGGAATT TTCCAATTTT TGATTTAATG 
GAAAATATAG GAAGAAAATG TGGCCGTATT CTGAGCCAGG TATCATACAG ACTGTTTGAA 
GACATGGGGC TCTTTGAAGC CTTTAAAATC CCGGTTAGGG AGTTTATGAA TTACTTTCAT 
GCTTTGGAGA TCGGATACAG GGACATTCCT TATCATAACA GAATCCATGC CACCGATGTT 
CTACACGCTG TGTGGTATCT CACAACACAG CCGATTCCTG GCCTCCCAAG TGTGATTGGT 
GATCACGGCT CAGCAAGCGA CTCCGATTCT GACAGTGGAT TTACACACGG ACACATGGGA 
TATGTGTTTT CAAAAATGTA TCACGTGCCA GATGACAAAT ATGGATGCCT GTCTGGGAAT 
ATTCCAGCCC TAGAGTTGAT GGCTCTGTAC GTTGCTGCAG CCATGCATGA CTACGATCAC 
CCAGGGAGGA CAAATGCCTT CCTGGTTGCC ACTAGTGCCC CTCAGGCGGT GCTATACAAC 
GACCGTTCTG TTCTGGAGAA CCATCACGCC GCTGCAGCCT GGAATCTCTT CATGTCCCGG 
CCAGAGTATA ACTTCTTAGT TAACCTGGAC CACGTGGAAT TCAAGCACTT CCGCTTCCTA 
GTCATTGAAG CGATTTTGGC TACTGACCTG AAGAAACACT TTGACTTTGT AGCCAAATTT 
AACGCCAAGG TAAACGATGA TGTCGGGATA GATTGGACCA ATGAAAACGA CCGTCTCCTG 
GTGTGTCAGA TGTGTATAAA GCTGGCTGAC ATCAACGGGC CTGCTAAATG CAAAGAACTC 
CACCTGCGGT GGACAGAGGG CATCGCCAGC GAGTTTTATG AGCAGGGCGA TGAAGAAGCC 
AGCCTCGGCT TGCCCATAAG TCCCTTTATG GACCGCTCTG CCCCGCAGCT GGCCAATCTT 
CAGGAATCCT TTATCTCACA CATCGTGGGT CCTCTCTGCC ACTCCTATGA CTCTGCGGGA 
CTCATGCCAG GAAAATGGGT AGATGACAGT GATGATTCCG GAGACACCGA TGACCCGGAA 
GAAGAGGAGG AAGAGGCTGA AACACCACAC GAGGACGAAG CCTGTGAAAG CAGTATAGCT 
CCCCGAAAGA AAAGTTTCAA AAGAAGGAGA ATCTACTGCC AAATAACTCA GCACCTTCTG 
CAGAACCACA TGATGTGGAA GAAAGTGATT GAAGAGGAAC AGTGTTTATC AGGCACAGAG 
AACCAGTCCC TGGACCAGGT CCCTCTGCAG CATCCCTCAG AGCAGATCCA GGCTATCAAG 
GAGGAAGAGG AAGAGAAGGG GAAGCCAAGA GCAGAGGAGA CCCTGGCTCC ACAGCCAGAC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507042.1
CDS	1514..4039
Translation	MQDQSSGMIGTSVQTYPKLMGHSEWDHKRGPRGSQSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPTVTFSENYTCSDSEEGLEKDKQAISKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSASIKPHEAPSPSAVNPDSWNAPGLTTLTKSRSFTSSYAVSAANHVKAKKQNRPGGLAKISPVPSPSSSPPQGSPASSPVSNSASQQFPESPEVTIKRGPGSHRALTYTQSAPDLSPQIPPPSVICSSCGRPYSQGNPADGPSERSGPAMLKPNRTDDTSQVTSDYETNNNSDSSDILQNEEEAECQREPQRKASACGTYTSQTMIFLDKPILAPEPLVMDNLDSIMDQLNTWNFPIFDLMENIGRKCGRILSQVSYRLFEDMGLFEAFKIPVREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLPSVIGDHGSASDSDSDSGFTHGHMGYVFSKMYHVPDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLVNLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNAKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLRWTEGIASEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCHSYDSAGLMPGKWVDDSDDSGDTDDPEEEEEEAETPHEDEACESSIAPRKKSFKRRRIYCQITQHLLQNHMMWKKVIEEEQCLSGTENQSLDQVPLQHPSEQIQAIKEEEEEKGKPRAEETLAPQPDL

Target ORF information:

RefSeq Version	XM_006506979.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506979.4

ORF Insert Sequence:

ATGCAGGACC AGTCGTCTGG GATGATAGGC ACTTCAGTGC AGACATATCC CAAGCTCATG 
GGCCATTCAG AATGGGACCA CAAGAGAGGG CCACGAGGAT CCCAGTCAGG AACCAGCATC 
ACTGTGGATA TTGCTGTGAT GGGTGAGGCC CATGGCCTCA TTACCGACCT CCTGGCCGAC 
CCTTCTCTGC CCCCGAATGT GTGTACATCC TTGAGGGCCG TAAGCAACTT GCTCAGCACC 
CAGCTCACCT TCCAGGCCAT CCACAAGCCC AGAGTGAACC CCACCGTGAC CTTCAGTGAG 
AACTATACCT GCTCGGACTC TGAGGAAGGC TTGGAGAAAG ACAAGCAGGC CATTTCCAAG 
CGCCTGAGAA GAAGTTTGCC TCCAGGCTTA CTGAGAAGAG TCTCGTCAAC TTGGACAACT 
ACCACCTCTG CCACAGGTCT GCCCACCTTG GAGCCTGCGC CAGTGCGGAG GGACCGCAGT 
GCCAGCATAA AGCCGCACGA AGCCCCTTCA CCCAGTGCTG TCAATCCTGA CTCTTGGAAT 
GCCCCAGGGT TGACGACTCT CACCAAAAGC AGATCCTTCA CTTCATCCTA TGCTGTCTCT 
GCAGCTAACC ATGTAAAAGC TAAAAAGCAA AACAGGCCAG GTGGCCTTGC TAAAATTTCC 
CCCGTCCCCT CACCCTCCTC CTCGCCTCCC CAGGGATCAC CTGCCAGCAG TCCTGTCAGC 
AACAGTGCTT CACAACAGTT CCCAGAATCC CCTGAAGTAA CCATCAAGCG AGGGCCTGGA 
TCTCACAGGG CCTTAACTTA CACCCAAAGT GCCCCTGACC TGTCCCCTCA GATCCCACCC 
CCGTCTGTCA TATGTAGCAG CTGTGGCAGA CCTTATTCGC AAGGGAATCC TGCTGATGGA 
CCCTCAGAGA GAAGTGGCCC AGCCATGCTG AAACCAAACA GAACAGATGA CACTTCTCAA 
GTTACCTCCG ATTATGAGAC CAACAACAAC AGTGACAGCA GTGACATTCT GCAGAATGAA 
GAGGAAGCTG AGTGCCAGAG AGAACCCCAG AGGAAAGCAT CGGCTTGTGG CACCTATACT 
TCCCAGACCA TGATCTTCCT GGACAAACCA ATTCTTGCTC CAGAACCCCT GGTCATGGAT 
AACCTGGACT CAATTATGGA TCAGTTGAAC ACCTGGAATT TTCCAATTTT TGATTTAATG 
GAAAATATAG GAAGAAAATG TGGCCGTATT CTGAGCCAGG TATCATACAG ACTGTTTGAA 
GACATGGGGC TCTTTGAAGC CTTTAAAATC CCGGTTAGGG AGTTTATGAA TTACTTTCAT 
GCTTTGGAGA TCGGATACAG GGACATTCCT TATCATAACA GAATCCATGC CACCGATGTT 
CTACACGCTG TGTGGTATCT CACAACACAG CCGATTCCTG GCCTCCCAAG TGTGATTGGT 
GATCACGGCT CAGCAAGCGA CTCCGATTCT GACAGTGGAT TTACACACGG ACACATGGGA 
TATGTGTTTT CAAAAATGTA TCACGTGCCA GATGACAAAT ATGGATGCCT GTCTGGGAAT 
ATTCCAGCCC TAGAGTTGAT GGCTCTGTAC GTTGCTGCAG CCATGCATGA CTACGATCAC 
CCAGGGAGGA CAAATGCCTT CCTGGTTGCC ACTAGTGCCC CTCAGGCGGT GCTATACAAC 
GACCGTTCTG TTCTGGAGAA CCATCACGCC GCTGCAGCCT GGAATCTCTT CATGTCCCGG 
CCAGAGTATA ACTTCTTAGT TAACCTGGAC CACGTGGAAT TCAAGCACTT CCGCTTCCTA 
GTCATTGAAG CGATTTTGGC TACTGACCTG AAGAAACACT TTGACTTTGT AGCCAAATTT 
AACGCCAAGG TAAACGATGA TGTCGGGATA GATTGGACCA ATGAAAACGA CCGTCTCCTG 
GTGTGTCAGA TGTGTATAAA GCTGGCTGAC ATCAACGGGC CTGCTAAATG CAAAGAACTC 
CACCTGCGGT GGACAGAGGG CATCGCCAGC GAGTTTTATG AGCAGGGCGA TGAAGAAGCC 
AGCCTCGGCT TGCCCATAAG TCCCTTTATG GACCGCTCTG CCCCGCAGCT GGCCAATCTT 
CAGGAATCCT TTATCTCACA CATCGTGGGT CCTCTCTGCC ACTCCTATGA CTCTGCGGGA 
CTCATGCCAG GAAAATGGGT AGATGACAGT GATGATTCCG GAGACACCGA TGACCCGGAA 
GAAGAGGAGG AAGAGGCTGA AACACCACAC GAGGACGAAG CCTGTGAAAG CAGTATAGCT 
CCCCGAAAGA AAAGTTTCAA AAGAAGGAGA ATCTACTGCC AAATAACTCA GCACCTTCTG 
CAGAACCACA TGATGTGGAA GAAAGTGATT GAAGAGGAAC AGTGTTTATC AGGCACAGAG 
AACCAGTCCC TGGACCAGGT CCCTCTGCAG CATCCCTCAG AGCAGATCCA GGCTATCAAG 
GAGGAAGAGG AAGAGAAGGG GAAGCCAAGA GCAGAGGAGA CCCTGGCTCC ACAGCCAGAC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde3a ( XM_011241595.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239897.1 Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69560
Clone ID Related Accession (Same CDS sequence)	XM_011241595.3 , XM_011241595.2
Accession Version	XM_011241595.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241595.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCCG TGCTGGCTCA GCATCTACTT GGTGCCATTT GTTTCTTCAG TGGCAGCTAT 
CTGACTCAGC TCATGGGCCA TTCAGAATGG GACCACAAGA GAGGGCCACG AGGATCCCAG 
TCAGGAACCA GCATCACTGT GGATATTGCT GTGATGGGTG AGGCCCATGG CCTCATTACC 
GACCTCCTGG CCGACCCTTC TCTGCCCCCG AATGTGTGTA CATCCTTGAG GGCCGTAAGC 
AACTTGCTCA GCACCCAGCT CACCTTCCAG GCCATCCACA AGCCCAGAGT GAACCCCACC 
GTGACCTTCA GTGAGAACTA TACCTGCTCG GACTCTGAGG AAGGCTTGGA GAAAGACAAG 
CAGGCCATTT CCAAGCGCCT GAGAAGAAGT TTGCCTCCAG GCTTACTGAG AAGAGTCTCG 
TCAACTTGGA CAACTACCAC CTCTGCCACA GGTCTGCCCA CCTTGGAGCC TGCGCCAGTG 
CGGAGGGACC GCAGTGCCAG CATAAAGCCG CACGAAGCCC CTTCACCCAG TGCTGTCAAT 
CCTGACTCTT GGAATGCCCC AGGGTTGACG ACTCTCACCA AAAGCAGATC CTTCACTTCA 
TCCTATGCTG TCTCTGCAGC TAACCATGTA AAAGCTAAAA AGCAAAACAG GCCAGGTGGC 
CTTGCTAAAA TTTCCCCCGT CCCCTCACCC TCCTCCTCGC CTCCCCAGGG ATCACCTGCC 
AGCAGTCCTG TCAGCAACAG TGCTTCACAA CAGTTCCCAG AATCCCCTGA AGTAACCATC 
AAGCGAGGGC CTGGATCTCA CAGGGCCTTA ACTTACACCC AAAGTGCCCC TGACCTGTCC 
CCTCAGATCC CACCCCCGTC TGTCATATGT AGCAGCTGTG GCAGACCTTA TTCGCAAGGG 
AATCCTGCTG ATGGACCCTC AGAGAGAAGT GGCCCAGCCA TGCTGAAACC AAACAGAACA 
GATGACACTT CTCAAGTTAC CTCCGATTAT GAGACCAACA ACAACAGTGA CAGCAGTGAC 
ATTCTGCAGA ATGAAGAGGA AGCTGAGTGC CAGAGAGAAC CCCAGAGGAA AGCATCGGCT 
TGTGGCACCT ATACTTCCCA GACCATGATC TTCCTGGACA AACCAATTCT TGCTCCAGAA 
CCCCTGGTCA TGGATAACCT GGACTCAATT ATGGATCAGT TGAACACCTG GAATTTTCCA 
ATTTTTGATT TAATGGAAAA TATAGGAAGA AAATGTGGCC GTATTCTGAG CCAGGTATCA 
TACAGACTGT TTGAAGACAT GGGGCTCTTT GAAGCCTTTA AAATCCCGGT TAGGGAGTTT 
ATGAATTACT TTCATGCTTT GGAGATCGGA TACAGGGACA TTCCTTATCA TAACAGAATC 
CATGCCACCG ATGTTCTACA CGCTGTGTGG TATCTCACAA CACAGCCGAT TCCTGGCCTC 
CCAAGTGTGA TTGGTGATCA CGGCTCAGCA AGCGACTCCG ATTCTGACAG TGGATTTACA 
CACGGACACA TGGGATATGT GTTTTCAAAA ATGTATCACG TGCCAGATGA CAAATATGGA 
TGCCTGTCTG GGAATATTCC AGCCCTAGAG TTGATGGCTC TGTACGTTGC TGCAGCCATG 
CATGACTACG ATCACCCAGG GAGGACAAAT GCCTTCCTGG TTGCCACTAG TGCCCCTCAG 
GCGGTGCTAT ACAACGACCG TTCTGTTCTG GAGAACCATC ACGCCGCTGC AGCCTGGAAT 
CTCTTCATGT CCCGGCCAGA GTATAACTTC TTAGTTAACC TGGACCACGT GGAATTCAAG 
CACTTCCGCT TCCTAGTCAT TGAAGCGATT TTGGCTACTG ACCTGAAGAA ACACTTTGAC 
TTTGTAGCCA AATTTAACGC CAAGGTAAAC GATGATGTCG GGATAGATTG GACCAATGAA 
AACGACCGTC TCCTGGTGTG TCAGATGTGT ATAAAGCTGG CTGACATCAA CGGGCCTGCT 
AAATGCAAAG AACTCCACCT GCGGTGGACA GAGGGCATCG CCAGCGAGTT TTATGAGCAG 
GGCGATGAAG AAGCCAGCCT CGGCTTGCCC ATAAGTCCCT TTATGGACCG CTCTGCCCCG 
CAGCTGGCCA ATCTTCAGGA ATCCTTTATC TCACACATCG TGGGTCCTCT CTGCCACTCC 
TATGACTCTG CGGGACTCAT GCCAGGAAAA TGGGTAGATG ACAGTGATGA TTCCGGAGAC 
ACCGATGACC CGGAAGAAGA GGAGGAAGAG GCTGAAACAC CACACGAGGA CGAAGCCTGT 
GAAAGCAGTA TAGCTCCCCG AAAGAAAAGT TTCAAAAGAA GGAGAATCTA CTGCCAAATA 
ACTCAGCACC TTCTGCAGAA CCACATGATG TGGAAGAAAG TGATTGAAGA GGAACAGTGT 
TTATCAGGCA CAGAGAACCA GTCCCTGGAC CAGGTCCCTC TGCAGCATCC CTCAGAGCAG 
ATCCAGGCTA TCAAGGAGGA AGAGGAAGAG AAGGGGAAGC CAAGAGCAGA GGAGACCCTG 
GCTCCACAGC CAGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239897.1
CDS	1356..3896
Translation	MKAVLAQHLLGAICFFSGSYLTQLMGHSEWDHKRGPRGSQSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPTVTFSENYTCSDSEEGLEKDKQAISKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSASIKPHEAPSPSAVNPDSWNAPGLTTLTKSRSFTSSYAVSAANHVKAKKQNRPGGLAKISPVPSPSSSPPQGSPASSPVSNSASQQFPESPEVTIKRGPGSHRALTYTQSAPDLSPQIPPPSVICSSCGRPYSQGNPADGPSERSGPAMLKPNRTDDTSQVTSDYETNNNSDSSDILQNEEEAECQREPQRKASACGTYTSQTMIFLDKPILAPEPLVMDNLDSIMDQLNTWNFPIFDLMENIGRKCGRILSQVSYRLFEDMGLFEAFKIPVREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLPSVIGDHGSASDSDSDSGFTHGHMGYVFSKMYHVPDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLVNLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNAKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLRWTEGIASEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCHSYDSAGLMPGKWVDDSDDSGDTDDPEEEEEEAETPHEDEACESSIAPRKKSFKRRRIYCQITQHLLQNHMMWKKVIEEEQCLSGTENQSLDQVPLQHPSEQIQAIKEEEEEKGKPRAEETLAPQPDL

Target ORF information:

RefSeq Version	XM_011241595.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241595.3

ORF Insert Sequence:

ATGAAGGCCG TGCTGGCTCA GCATCTACTT GGTGCCATTT GTTTCTTCAG TGGCAGCTAT 
CTGACTCAGC TCATGGGCCA TTCAGAATGG GACCACAAGA GAGGGCCACG AGGATCCCAG 
TCAGGAACCA GCATCACTGT GGATATTGCT GTGATGGGTG AGGCCCATGG CCTCATTACC 
GACCTCCTGG CCGACCCTTC TCTGCCCCCG AATGTGTGTA CATCCTTGAG GGCCGTAAGC 
AACTTGCTCA GCACCCAGCT CACCTTCCAG GCCATCCACA AGCCCAGAGT GAACCCCACC 
GTGACCTTCA GTGAGAACTA TACCTGCTCG GACTCTGAGG AAGGCTTGGA GAAAGACAAG 
CAGGCCATTT CCAAGCGCCT GAGAAGAAGT TTGCCTCCAG GCTTACTGAG AAGAGTCTCG 
TCAACTTGGA CAACTACCAC CTCTGCCACA GGTCTGCCCA CCTTGGAGCC TGCGCCAGTG 
CGGAGGGACC GCAGTGCCAG CATAAAGCCG CACGAAGCCC CTTCACCCAG TGCTGTCAAT 
CCTGACTCTT GGAATGCCCC AGGGTTGACG ACTCTCACCA AAAGCAGATC CTTCACTTCA 
TCCTATGCTG TCTCTGCAGC TAACCATGTA AAAGCTAAAA AGCAAAACAG GCCAGGTGGC 
CTTGCTAAAA TTTCCCCCGT CCCCTCACCC TCCTCCTCGC CTCCCCAGGG ATCACCTGCC 
AGCAGTCCTG TCAGCAACAG TGCTTCACAA CAGTTCCCAG AATCCCCTGA AGTAACCATC 
AAGCGAGGGC CTGGATCTCA CAGGGCCTTA ACTTACACCC AAAGTGCCCC TGACCTGTCC 
CCTCAGATCC CACCCCCGTC TGTCATATGT AGCAGCTGTG GCAGACCTTA TTCGCAAGGG 
AATCCTGCTG ATGGACCCTC AGAGAGAAGT GGCCCAGCCA TGCTGAAACC AAACAGAACA 
GATGACACTT CTCAAGTTAC CTCCGATTAT GAGACCAACA ACAACAGTGA CAGCAGTGAC 
ATTCTGCAGA ATGAAGAGGA AGCTGAGTGC CAGAGAGAAC CCCAGAGGAA AGCATCGGCT 
TGTGGCACCT ATACTTCCCA GACCATGATC TTCCTGGACA AACCAATTCT TGCTCCAGAA 
CCCCTGGTCA TGGATAACCT GGACTCAATT ATGGATCAGT TGAACACCTG GAATTTTCCA 
ATTTTTGATT TAATGGAAAA TATAGGAAGA AAATGTGGCC GTATTCTGAG CCAGGTATCA 
TACAGACTGT TTGAAGACAT GGGGCTCTTT GAAGCCTTTA AAATCCCGGT TAGGGAGTTT 
ATGAATTACT TTCATGCTTT GGAGATCGGA TACAGGGACA TTCCTTATCA TAACAGAATC 
CATGCCACCG ATGTTCTACA CGCTGTGTGG TATCTCACAA CACAGCCGAT TCCTGGCCTC 
CCAAGTGTGA TTGGTGATCA CGGCTCAGCA AGCGACTCCG ATTCTGACAG TGGATTTACA 
CACGGACACA TGGGATATGT GTTTTCAAAA ATGTATCACG TGCCAGATGA CAAATATGGA 
TGCCTGTCTG GGAATATTCC AGCCCTAGAG TTGATGGCTC TGTACGTTGC TGCAGCCATG 
CATGACTACG ATCACCCAGG GAGGACAAAT GCCTTCCTGG TTGCCACTAG TGCCCCTCAG 
GCGGTGCTAT ACAACGACCG TTCTGTTCTG GAGAACCATC ACGCCGCTGC AGCCTGGAAT 
CTCTTCATGT CCCGGCCAGA GTATAACTTC TTAGTTAACC TGGACCACGT GGAATTCAAG 
CACTTCCGCT TCCTAGTCAT TGAAGCGATT TTGGCTACTG ACCTGAAGAA ACACTTTGAC 
TTTGTAGCCA AATTTAACGC CAAGGTAAAC GATGATGTCG GGATAGATTG GACCAATGAA 
AACGACCGTC TCCTGGTGTG TCAGATGTGT ATAAAGCTGG CTGACATCAA CGGGCCTGCT 
AAATGCAAAG AACTCCACCT GCGGTGGACA GAGGGCATCG CCAGCGAGTT TTATGAGCAG 
GGCGATGAAG AAGCCAGCCT CGGCTTGCCC ATAAGTCCCT TTATGGACCG CTCTGCCCCG 
CAGCTGGCCA ATCTTCAGGA ATCCTTTATC TCACACATCG TGGGTCCTCT CTGCCACTCC 
TATGACTCTG CGGGACTCAT GCCAGGAAAA TGGGTAGATG ACAGTGATGA TTCCGGAGAC 
ACCGATGACC CGGAAGAAGA GGAGGAAGAG GCTGAAACAC CACACGAGGA CGAAGCCTGT 
GAAAGCAGTA TAGCTCCCCG AAAGAAAAGT TTCAAAAGAA GGAGAATCTA CTGCCAAATA 
ACTCAGCACC TTCTGCAGAA CCACATGATG TGGAAGAAAG TGATTGAAGA GGAACAGTGT 
TTATCAGGCA CAGAGAACCA GTCCCTGGAC CAGGTCCCTC TGCAGCATCC CTCAGAGCAG 
ATCCAGGCTA TCAAGGAGGA AGAGGAAGAG AAGGGGAAGC CAAGAGCAGA GGAGACCCTG 
GCTCCACAGC CAGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde3a ( NM_018779.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061249.1 Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), mRNA.

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CloneID	OMu10996
Clone ID Related Accession (Same CDS sequence)	NM_018779.1 , NM_018779.2
Accession Version	NM_018779.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF099187.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF099187.1, BC138265.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGTGC GGGGCGAGGC CGCCCAAGAC CTAGCCAAGC CGGGCCTCGG AGGGGCGAGT 
CCCGCCCGTG TGGCAAGAGG GAACCACCGC CATCGTGGCG AGTCCTCTCC ATCCCCTCGG 
GGCTCCGGCT GTTGTTGGAG AGCCCTGGCG CTGCAGCCGC TCCGGCGCTC GCCTCAACTC 
TCCTCGGCGC TGTGCGCGGG CTCCCTGTCC GTGCTGCTGG CGCTGCTGGT GAGGCTGGTC 
GGCGGGGAGG TCGGAGGCGA GCTGGAGAAG AGTCAGGAGG CGGCGGCCGA GGAGGAGGAG 
GAGGAAGGAG CCCGAGGGGG CGTCTTCCCG GGCCCTCGGG GAGGTGCTCC CGGGGGCGGC 
GCGCAGCTCA GCCCTTGGCT GCAGCCGGCC GCGCTGCTCT TCAGTCTCCT GTGCGCCTTC 
TTCTGGATGG GCTTGTGCCT GCTGCGCGCC GGGGTGCGCC TGCCTCTGGC CGTCGCGCTG 
TTGGCCGCCT GCTGCGCCGG GGAAGCGCTG GTGCAGCTCT CCTTGGGCGT GGGGGACGGT 
CGCCTGCTGT CGCTGCCCGC CGCGGGGGTC CTGCTCAGCT GCCTAGGTGG CGCGACATGG 
CTGGTGCTGA GGCTGAGGCT GGGCGTCCTC ATGGTCGCCT GGACTAGCGT GCTTAGGACG 
GTGGCCCTGG TTTCTCTGGA GAGGTTCAAG GTCGCCTGGA GACCTTACCT GGCATACTTG 
GCGGCCGTGC TTGGGCTGCT GCTGGCCAGG TACGCCGAGC AGATCCTGCC GCAGTGCTCC 
GGGCCGGCTC CGCCCAGGGA GCGGTTTGGG TCCCAGTTGA GCGCGAGGAC CAAGGAAGAG 
ATTCCGGGGT GGAAGAGGAG GAGGCGGTCC AGCTCGGTGG TGGCCGGCGA GATGTCCGGC 
TGCAGTGGCA AGTCTCACCG GAGGACCTCC CTGCCCTGCA TACCCAGGGA GCAGCTCATG 
GGCCATTCAG AATGGGACCA CAAGAGAGGG CCACGAGGAT CCCAGTCAGG AACCAGCATC 
ACTGTGGATA TTGCTGTGAT GGGTGAGGCC CATGGCCTCA TTACCGACCT CCTGGCCGAC 
CCTTCTCTGC CCCCGAATGT GTGTACATCC TTGAGGGCCG TAAGCAACTT GCTCAGCACC 
CAGCTCACCT TCCAGGCCAT CCACAAGCCC AGAGTGAACC CCACCGTGAC CTTCAGTGAG 
AACTATACCT GCTCGGACTC TGAGGAAGGC TTGGAGAAAG ACAAGCAGGC CATTTCCAAG 
CGCCTGAGAA GAAGTTTGCC TCCAGGCTTA CTGAGAAGAG TCTCGTCAAC TTGGACAACT 
ACCACCTCTG CCACAGGTCT GCCCACCTTG GAGCCTGCGC CAGTGCGGAG GGACCGCAGT 
GCCAGCATAA AGCCGCACGA AGCCCCTTCA CCCAGTGCTG TCAATCCTGA CTCTTGGAAT 
GCCCCAGGGT TGACGACTCT CACCAAAAGC AGATCCTTCA CTTCATCCTA TGCTGTCTCT 
GCAGCTAACC ATGTAAAAGC TAAAAAGCAA AACAGGCCAG GTGGCCTTGC TAAAATTTCC 
CCCGTCCCCT CACCCTCCTC CTCGCCTCCC CAGGGATCAC CTGCCAGCAG TCCTGTCAGC 
AACAGTGCTT CACAACAGTT CCCAGAATCC CCTGAAGTAA CCATCAAGCG AGGGCCTGGA 
TCTCACAGGG CCTTAACTTA CACCCAAAGT GCCCCTGACC TGTCCCCTCA GATCCCACCC 
CCGTCTGTCA TATGTAGCAG CTGTGGCAGA CCTTATTCGC AAGGGAATCC TGCTGATGGA 
CCCTCAGAGA GAAGTGGCCC AGCCATGCTG AAACCAAACA GAACAGATGA CACTTCTCAA 
GTTACCTCCG ATTATGAGAC CAACAACAAC AGTGACAGCA GTGACATTCT GCAGAATGAA 
GAGGAAGCTG AGTGCCAGAG AGAACCCCAG AGGAAAGCAT CGGCTTGTGG CACCTATACT 
TCCCAGACCA TGATCTTCCT GGACAAACCA ATTCTTGCTC CAGAACCCCT GGTCATGGAT 
AACCTGGACT CAATTATGGA TCAGTTGAAC ACCTGGAATT TTCCAATTTT TGATTTAATG 
GAAAATATAG GAAGAAAATG TGGCCGTATT CTGAGCCAGG TATCATACAG ACTGTTTGAA 
GACATGGGGC TCTTTGAAGC CTTTAAAATC CCGGTTAGGG AGTTTATGAA TTACTTTCAT 
GCTTTGGAGA TCGGATACAG GGACATTCCT TATCATAACA GAATCCATGC CACCGATGTT 
CTACACGCTG TGTGGTATCT CACAACACAG CCGATTCCTG GCCTCCCAAG TGTGATTGGT 
GATCACGGCT CAGCAAGCGA CTCCGATTCT GACAGTGGAT TTACACACGG ACACATGGGA 
TATGTGTTTT CAAAAATGTA TCACGTGCCA GATGACAAAT ATGGATGCCT GTCTGGGAAT 
ATTCCAGCCC TAGAGTTGAT GGCTCTGTAC GTTGCTGCAG CCATGCATGA CTACGATCAC 
CCAGGGAGGA CAAATGCCTT CCTGGTTGCC ACTAGTGCCC CTCAGGCGGT GCTATACAAC 
GACCGTTCTG TTCTGGAGAA CCATCACGCC GCTGCAGCCT GGAATCTCTT CATGTCCCGG 
CCAGAGTATA ACTTCTTAGT TAACCTGGAC CACGTGGAAT TCAAGCACTT CCGCTTCCTA 
GTCATTGAAG CGATTTTGGC TACTGACCTG AAGAAACACT TTGACTTTGT AGCCAAATTT 
AACGCCAAGG TAAACGATGA TGTCGGGATA GATTGGACCA ATGAAAACGA CCGTCTCCTG 
GTGTGTCAGA TGTGTATAAA GCTGGCTGAC ATCAACGGGC CTGCTAAATG CAAAGAACTC 
CACCTGCGGT GGACAGAGGG CATCGCCAGC GAGTTTTATG AGCAGGGCGA TGAAGAAGCC 
AGCCTCGGCT TGCCCATAAG TCCCTTTATG GACCGCTCTG CCCCGCAGCT GGCCAATCTT 
CAGGAATCCT TTATCTCACA CATCGTGGGT CCTCTCTGCC ACTCCTATGA CTCTGCGGGA 
CTCATGCCAG GAAAATGGGT AGATGACAGT GATGATTCCG GAGACACCGA TGACCCGGAA 
GAAGAGGAGG AAGAGGCTGA AACACCACAC GAGGACGAAG CCTGTGAAAG CAGTATAGCT 
CCCCGAAAGA AAAGTTTCAA AAGAAGGAGA ATCTACTGCC AAATAACTCA GCACCTTCTG 
CAGAACCACA TGATGTGGAA GAAAGTGATT GAAGAGGAAC AGTGTTTATC AGGCACAGAG 
AACCAGTCCC TGGACCAGGT CCCTCTGCAG CATCCCTCAG AGCAGATCCA GGCTATCAAG 
GAGGAAGAGG AAGAGAAGGG GAAGCCAAGA GCAGAGGAGA CCCTGGCTCC ACAGCCAGAC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061249.1
CDS	322..3747
Translation	MAVRGEAAQDLAKPGLGGASPARVARGNHRHRGESSPSPRGSGCCWRALALQPLRRSPQLSSALCAGSLSVLLALLVRLVGGEVGGELEKSQEAAAEEEEEEGARGGVFPGPRGGAPGGGAQLSPWLQPAALLFSLLCAFFWMGLCLLRAGVRLPLAVALLAACCAGEALVQLSLGVGDGRLLSLPAAGVLLSCLGGATWLVLRLRLGVLMVAWTSVLRTVALVSLERFKVAWRPYLAYLAAVLGLLLARYAEQILPQCSGPAPPRERFGSQLSARTKEEIPGWKRRRRSSSVVAGEMSGCSGKSHRRTSLPCIPREQLMGHSEWDHKRGPRGSQSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPTVTFSENYTCSDSEEGLEKDKQAISKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSASIKPHEAPSPSAVNPDSWNAPGLTTLTKSRSFTSSYAVSAANHVKAKKQNRPGGLAKISPVPSPSSSPPQGSPASSPVSNSASQQFPESPEVTIKRGPGSHRALTYTQSAPDLSPQIPPPSVICSSCGRPYSQGNPADGPSERSGPAMLKPNRTDDTSQVTSDYETNNNSDSSDILQNEEEAECQREPQRKASACGTYTSQTMIFLDKPILAPEPLVMDNLDSIMDQLNTWNFPIFDLMENIGRKCGRILSQVSYRLFEDMGLFEAFKIPVREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLPSVIGDHGSASDSDSDSGFTHGHMGYVFSKMYHVPDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLVNLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNAKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLRWTEGIASEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCHSYDSAGLMPGKWVDDSDDSGDTDDPEEEEEEAETPHEDEACESSIAPRKKSFKRRRIYCQITQHLLQNHMMWKKVIEEEQCLSGTENQSLDQVPLQHPSEQIQAIKEEEEEKGKPRAEETLAPQPDL

Target ORF information:

RefSeq Version	NM_018779.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018779.1

ORF Insert Sequence:

ATGGCAGTGC GGGGCGAGGC CGCCCAAGAC CTAGCCAAGC CGGGCCTCGG AGGGGCGAGT 
CCCGCCCGTG TGGCAAGAGG GAACCACCGC CATCGTGGCG AGTCCTCTCC ATCCCCTCGG 
GGCTCCGGCT GTTGTTGGAG AGCCCTGGCG CTGCAGCCGC TCCGGCGCTC GCCTCAACTC 
TCCTCGGCGC TGTGCGCGGG CTCCCTGTCC GTGCTGCTGG CGCTGCTGGT GAGGCTGGTC 
GGCGGGGAGG TCGGAGGCGA GCTGGAGAAG AGTCAGGAGG CGGCGGCCGA GGAGGAGGAG 
GAGGAAGGAG CCCGAGGGGG CGTCTTCCCG GGCCCTCGGG GAGGTGCTCC CGGGGGCGGC 
GCGCAGCTCA GCCCTTGGCT GCAGCCGGCC GCGCTGCTCT TCAGTCTCCT GTGCGCCTTC 
TTCTGGATGG GCTTGTGCCT GCTGCGCGCC GGGGTGCGCC TGCCTCTGGC CGTCGCGCTG 
TTGGCCGCCT GCTGCGCCGG GGAAGCGCTG GTGCAGCTCT CCTTGGGCGT GGGGGACGGT 
CGCCTGCTGT CGCTGCCCGC CGCGGGGGTC CTGCTCAGCT GCCTAGGTGG CGCGACATGG 
CTGGTGCTGA GGCTGAGGCT GGGCGTCCTC ATGGTCGCCT GGACTAGCGT GCTTAGGACG 
GTGGCCCTGG TTTCTCTGGA GAGGTTCAAG GTCGCCTGGA GACCTTACCT GGCATACTTG 
GCGGCCGTGC TTGGGCTGCT GCTGGCCAGG TACGCCGAGC AGATCCTGCC GCAGTGCTCC 
GGGCCGGCTC CGCCCAGGGA GCGGTTTGGG TCCCAGTTGA GCGCGAGGAC CAAGGAAGAG 
ATTCCGGGGT GGAAGAGGAG GAGGCGGTCC AGCTCGGTGG TGGCCGGCGA GATGTCCGGC 
TGCAGTGGCA AGTCTCACCG GAGGACCTCC CTGCCCTGCA TACCCAGGGA GCAGCTCATG 
GGCCATTCAG AATGGGACCA CAAGAGAGGG CCACGAGGAT CCCAGTCAGG AACCAGCATC 
ACTGTGGATA TTGCTGTGAT GGGTGAGGCC CATGGCCTCA TTACCGACCT CCTGGCCGAC 
CCTTCTCTGC CCCCGAATGT GTGTACATCC TTGAGGGCCG TAAGCAACTT GCTCAGCACC 
CAGCTCACCT TCCAGGCCAT CCACAAGCCC AGAGTGAACC CCACCGTGAC CTTCAGTGAG 
AACTATACCT GCTCGGACTC TGAGGAAGGC TTGGAGAAAG ACAAGCAGGC CATTTCCAAG 
CGCCTGAGAA GAAGTTTGCC TCCAGGCTTA CTGAGAAGAG TCTCGTCAAC TTGGACAACT 
ACCACCTCTG CCACAGGTCT GCCCACCTTG GAGCCTGCGC CAGTGCGGAG GGACCGCAGT 
GCCAGCATAA AGCCGCACGA AGCCCCTTCA CCCAGTGCTG TCAATCCTGA CTCTTGGAAT 
GCCCCAGGGT TGACGACTCT CACCAAAAGC AGATCCTTCA CTTCATCCTA TGCTGTCTCT 
GCAGCTAACC ATGTAAAAGC TAAAAAGCAA AACAGGCCAG GTGGCCTTGC TAAAATTTCC 
CCCGTCCCCT CACCCTCCTC CTCGCCTCCC CAGGGATCAC CTGCCAGCAG TCCTGTCAGC 
AACAGTGCTT CACAACAGTT CCCAGAATCC CCTGAAGTAA CCATCAAGCG AGGGCCTGGA 
TCTCACAGGG CCTTAACTTA CACCCAAAGT GCCCCTGACC TGTCCCCTCA GATCCCACCC 
CCGTCTGTCA TATGTAGCAG CTGTGGCAGA CCTTATTCGC AAGGGAATCC TGCTGATGGA 
CCCTCAGAGA GAAGTGGCCC AGCCATGCTG AAACCAAACA GAACAGATGA CACTTCTCAA 
GTTACCTCCG ATTATGAGAC CAACAACAAC AGTGACAGCA GTGACATTCT GCAGAATGAA 
GAGGAAGCTG AGTGCCAGAG AGAACCCCAG AGGAAAGCAT CGGCTTGTGG CACCTATACT 
TCCCAGACCA TGATCTTCCT GGACAAACCA ATTCTTGCTC CAGAACCCCT GGTCATGGAT 
AACCTGGACT CAATTATGGA TCAGTTGAAC ACCTGGAATT TTCCAATTTT TGATTTAATG 
GAAAATATAG GAAGAAAATG TGGCCGTATT CTGAGCCAGG TATCATACAG ACTGTTTGAA 
GACATGGGGC TCTTTGAAGC CTTTAAAATC CCGGTTAGGG AGTTTATGAA TTACTTTCAT 
GCTTTGGAGA TCGGATACAG GGACATTCCT TATCATAACA GAATCCATGC CACCGATGTT 
CTACACGCTG TGTGGTATCT CACAACACAG CCGATTCCTG GCCTCCCAAG TGTGATTGGT 
GATCACGGCT CAGCAAGCGA CTCCGATTCT GACAGTGGAT TTACACACGG ACACATGGGA 
TATGTGTTTT CAAAAATGTA TCACGTGCCA GATGACAAAT ATGGATGCCT GTCTGGGAAT 
ATTCCAGCCC TAGAGTTGAT GGCTCTGTAC GTTGCTGCAG CCATGCATGA CTACGATCAC 
CCAGGGAGGA CAAATGCCTT CCTGGTTGCC ACTAGTGCCC CTCAGGCGGT GCTATACAAC 
GACCGTTCTG TTCTGGAGAA CCATCACGCC GCTGCAGCCT GGAATCTCTT CATGTCCCGG 
CCAGAGTATA ACTTCTTAGT TAACCTGGAC CACGTGGAAT TCAAGCACTT CCGCTTCCTA 
GTCATTGAAG CGATTTTGGC TACTGACCTG AAGAAACACT TTGACTTTGT AGCCAAATTT 
AACGCCAAGG TAAACGATGA TGTCGGGATA GATTGGACCA ATGAAAACGA CCGTCTCCTG 
GTGTGTCAGA TGTGTATAAA GCTGGCTGAC ATCAACGGGC CTGCTAAATG CAAAGAACTC 
CACCTGCGGT GGACAGAGGG CATCGCCAGC GAGTTTTATG AGCAGGGCGA TGAAGAAGCC 
AGCCTCGGCT TGCCCATAAG TCCCTTTATG GACCGCTCTG CCCCGCAGCT GGCCAATCTT 
CAGGAATCCT TTATCTCACA CATCGTGGGT CCTCTCTGCC ACTCCTATGA CTCTGCGGGA 
CTCATGCCAG GAAAATGGGT AGATGACAGT GATGATTCCG GAGACACCGA TGACCCGGAA 
GAAGAGGAGG AAGAGGCTGA AACACCACAC GAGGACGAAG CCTGTGAAAG CAGTATAGCT 
CCCCGAAAGA AAAGTTTCAA AAGAAGGAGA ATCTACTGCC AAATAACTCA GCACCTTCTG 
CAGAACCACA TGATGTGGAA GAAAGTGATT GAAGAGGAAC AGTGTTTATC AGGCACAGAG 
AACCAGTCCC TGGACCAGGT CCCTCTGCAG CATCCCTCAG AGCAGATCCA GGCTATCAAG 
GAGGAAGAGG AAGAGAAGGG GAAGCCAAGA GCAGAGGAGA CCCTGGCTCC ACAGCCAGAC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde3a ( NM_018779.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061249.1 Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), mRNA.

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CloneID	OMu10996
Clone ID Related Accession (Same CDS sequence)	NM_018779.1 , NM_018779.2
Accession Version	NM_018779.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF099187.1 and AC155835.3. On May 29, 2018 this sequence version replaced NM_018779.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF099187.1, AK052374.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGTGC GGGGCGAGGC CGCCCAAGAC CTAGCCAAGC CGGGCCTCGG AGGGGCGAGT 
CCCGCCCGTG TGGCAAGAGG GAACCACCGC CATCGTGGCG AGTCCTCTCC ATCCCCTCGG 
GGCTCCGGCT GTTGTTGGAG AGCCCTGGCG CTGCAGCCGC TCCGGCGCTC GCCTCAACTC 
TCCTCGGCGC TGTGCGCGGG CTCCCTGTCC GTGCTGCTGG CGCTGCTGGT GAGGCTGGTC 
GGCGGGGAGG TCGGAGGCGA GCTGGAGAAG AGTCAGGAGG CGGCGGCCGA GGAGGAGGAG 
GAGGAAGGAG CCCGAGGGGG CGTCTTCCCG GGCCCTCGGG GAGGTGCTCC CGGGGGCGGC 
GCGCAGCTCA GCCCTTGGCT GCAGCCGGCC GCGCTGCTCT TCAGTCTCCT GTGCGCCTTC 
TTCTGGATGG GCTTGTGCCT GCTGCGCGCC GGGGTGCGCC TGCCTCTGGC CGTCGCGCTG 
TTGGCCGCCT GCTGCGCCGG GGAAGCGCTG GTGCAGCTCT CCTTGGGCGT GGGGGACGGT 
CGCCTGCTGT CGCTGCCCGC CGCGGGGGTC CTGCTCAGCT GCCTAGGTGG CGCGACATGG 
CTGGTGCTGA GGCTGAGGCT GGGCGTCCTC ATGGTCGCCT GGACTAGCGT GCTTAGGACG 
GTGGCCCTGG TTTCTCTGGA GAGGTTCAAG GTCGCCTGGA GACCTTACCT GGCATACTTG 
GCGGCCGTGC TTGGGCTGCT GCTGGCCAGG TACGCCGAGC AGATCCTGCC GCAGTGCTCC 
GGGCCGGCTC CGCCCAGGGA GCGGTTTGGG TCCCAGTTGA GCGCGAGGAC CAAGGAAGAG 
ATTCCGGGGT GGAAGAGGAG GAGGCGGTCC AGCTCGGTGG TGGCCGGCGA GATGTCCGGC 
TGCAGTGGCA AGTCTCACCG GAGGACCTCC CTGCCCTGCA TACCCAGGGA GCAGCTCATG 
GGCCATTCAG AATGGGACCA CAAGAGAGGG CCACGAGGAT CCCAGTCAGG AACCAGCATC 
ACTGTGGATA TTGCTGTGAT GGGTGAGGCC CATGGCCTCA TTACCGACCT CCTGGCCGAC 
CCTTCTCTGC CCCCGAATGT GTGTACATCC TTGAGGGCCG TAAGCAACTT GCTCAGCACC 
CAGCTCACCT TCCAGGCCAT CCACAAGCCC AGAGTGAACC CCACCGTGAC CTTCAGTGAG 
AACTATACCT GCTCGGACTC TGAGGAAGGC TTGGAGAAAG ACAAGCAGGC CATTTCCAAG 
CGCCTGAGAA GAAGTTTGCC TCCAGGCTTA CTGAGAAGAG TCTCGTCAAC TTGGACAACT 
ACCACCTCTG CCACAGGTCT GCCCACCTTG GAGCCTGCGC CAGTGCGGAG GGACCGCAGT 
GCCAGCATAA AGCCGCACGA AGCCCCTTCA CCCAGTGCTG TCAATCCTGA CTCTTGGAAT 
GCCCCAGGGT TGACGACTCT CACCAAAAGC AGATCCTTCA CTTCATCCTA TGCTGTCTCT 
GCAGCTAACC ATGTAAAAGC TAAAAAGCAA AACAGGCCAG GTGGCCTTGC TAAAATTTCC 
CCCGTCCCCT CACCCTCCTC CTCGCCTCCC CAGGGATCAC CTGCCAGCAG TCCTGTCAGC 
AACAGTGCTT CACAACAGTT CCCAGAATCC CCTGAAGTAA CCATCAAGCG AGGGCCTGGA 
TCTCACAGGG CCTTAACTTA CACCCAAAGT GCCCCTGACC TGTCCCCTCA GATCCCACCC 
CCGTCTGTCA TATGTAGCAG CTGTGGCAGA CCTTATTCGC AAGGGAATCC TGCTGATGGA 
CCCTCAGAGA GAAGTGGCCC AGCCATGCTG AAACCAAACA GAACAGATGA CACTTCTCAA 
GTTACCTCCG ATTATGAGAC CAACAACAAC AGTGACAGCA GTGACATTCT GCAGAATGAA 
GAGGAAGCTG AGTGCCAGAG AGAACCCCAG AGGAAAGCAT CGGCTTGTGG CACCTATACT 
TCCCAGACCA TGATCTTCCT GGACAAACCA ATTCTTGCTC CAGAACCCCT GGTCATGGAT 
AACCTGGACT CAATTATGGA TCAGTTGAAC ACCTGGAATT TTCCAATTTT TGATTTAATG 
GAAAATATAG GAAGAAAATG TGGCCGTATT CTGAGCCAGG TATCATACAG ACTGTTTGAA 
GACATGGGGC TCTTTGAAGC CTTTAAAATC CCGGTTAGGG AGTTTATGAA TTACTTTCAT 
GCTTTGGAGA TCGGATACAG GGACATTCCT TATCATAACA GAATCCATGC CACCGATGTT 
CTACACGCTG TGTGGTATCT CACAACACAG CCGATTCCTG GCCTCCCAAG TGTGATTGGT 
GATCACGGCT CAGCAAGCGA CTCCGATTCT GACAGTGGAT TTACACACGG ACACATGGGA 
TATGTGTTTT CAAAAATGTA TCACGTGCCA GATGACAAAT ATGGATGCCT GTCTGGGAAT 
ATTCCAGCCC TAGAGTTGAT GGCTCTGTAC GTTGCTGCAG CCATGCATGA CTACGATCAC 
CCAGGGAGGA CAAATGCCTT CCTGGTTGCC ACTAGTGCCC CTCAGGCGGT GCTATACAAC 
GACCGTTCTG TTCTGGAGAA CCATCACGCC GCTGCAGCCT GGAATCTCTT CATGTCCCGG 
CCAGAGTATA ACTTCTTAGT TAACCTGGAC CACGTGGAAT TCAAGCACTT CCGCTTCCTA 
GTCATTGAAG CGATTTTGGC TACTGACCTG AAGAAACACT TTGACTTTGT AGCCAAATTT 
AACGCCAAGG TAAACGATGA TGTCGGGATA GATTGGACCA ATGAAAACGA CCGTCTCCTG 
GTGTGTCAGA TGTGTATAAA GCTGGCTGAC ATCAACGGGC CTGCTAAATG CAAAGAACTC 
CACCTGCGGT GGACAGAGGG CATCGCCAGC GAGTTTTATG AGCAGGGCGA TGAAGAAGCC 
AGCCTCGGCT TGCCCATAAG TCCCTTTATG GACCGCTCTG CCCCGCAGCT GGCCAATCTT 
CAGGAATCCT TTATCTCACA CATCGTGGGT CCTCTCTGCC ACTCCTATGA CTCTGCGGGA 
CTCATGCCAG GAAAATGGGT AGATGACAGT GATGATTCCG GAGACACCGA TGACCCGGAA 
GAAGAGGAGG AAGAGGCTGA AACACCACAC GAGGACGAAG CCTGTGAAAG CAGTATAGCT 
CCCCGAAAGA AAAGTTTCAA AAGAAGGAGA ATCTACTGCC AAATAACTCA GCACCTTCTG 
CAGAACCACA TGATGTGGAA GAAAGTGATT GAAGAGGAAC AGTGTTTATC AGGCACAGAG 
AACCAGTCCC TGGACCAGGT CCCTCTGCAG CATCCCTCAG AGCAGATCCA GGCTATCAAG 
GAGGAAGAGG AAGAGAAGGG GAAGCCAAGA GCAGAGGAGA CCCTGGCTCC ACAGCCAGAC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061249.1
CDS	322..3747
Translation	MAVRGEAAQDLAKPGLGGASPARVARGNHRHRGESSPSPRGSGCCWRALALQPLRRSPQLSSALCAGSLSVLLALLVRLVGGEVGGELEKSQEAAAEEEEEEGARGGVFPGPRGGAPGGGAQLSPWLQPAALLFSLLCAFFWMGLCLLRAGVRLPLAVALLAACCAGEALVQLSLGVGDGRLLSLPAAGVLLSCLGGATWLVLRLRLGVLMVAWTSVLRTVALVSLERFKVAWRPYLAYLAAVLGLLLARYAEQILPQCSGPAPPRERFGSQLSARTKEEIPGWKRRRRSSSVVAGEMSGCSGKSHRRTSLPCIPREQLMGHSEWDHKRGPRGSQSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPTVTFSENYTCSDSEEGLEKDKQAISKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSASIKPHEAPSPSAVNPDSWNAPGLTTLTKSRSFTSSYAVSAANHVKAKKQNRPGGLAKISPVPSPSSSPPQGSPASSPVSNSASQQFPESPEVTIKRGPGSHRALTYTQSAPDLSPQIPPPSVICSSCGRPYSQGNPADGPSERSGPAMLKPNRTDDTSQVTSDYETNNNSDSSDILQNEEEAECQREPQRKASACGTYTSQTMIFLDKPILAPEPLVMDNLDSIMDQLNTWNFPIFDLMENIGRKCGRILSQVSYRLFEDMGLFEAFKIPVREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLPSVIGDHGSASDSDSDSGFTHGHMGYVFSKMYHVPDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLVNLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNAKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLRWTEGIASEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCHSYDSAGLMPGKWVDDSDDSGDTDDPEEEEEEAETPHEDEACESSIAPRKKSFKRRRIYCQITQHLLQNHMMWKKVIEEEQCLSGTENQSLDQVPLQHPSEQIQAIKEEEEEKGKPRAEETLAPQPDL

Target ORF information:

RefSeq Version	NM_018779.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018779.2

ORF Insert Sequence:

ATGGCAGTGC GGGGCGAGGC CGCCCAAGAC CTAGCCAAGC CGGGCCTCGG AGGGGCGAGT 
CCCGCCCGTG TGGCAAGAGG GAACCACCGC CATCGTGGCG AGTCCTCTCC ATCCCCTCGG 
GGCTCCGGCT GTTGTTGGAG AGCCCTGGCG CTGCAGCCGC TCCGGCGCTC GCCTCAACTC 
TCCTCGGCGC TGTGCGCGGG CTCCCTGTCC GTGCTGCTGG CGCTGCTGGT GAGGCTGGTC 
GGCGGGGAGG TCGGAGGCGA GCTGGAGAAG AGTCAGGAGG CGGCGGCCGA GGAGGAGGAG 
GAGGAAGGAG CCCGAGGGGG CGTCTTCCCG GGCCCTCGGG GAGGTGCTCC CGGGGGCGGC 
GCGCAGCTCA GCCCTTGGCT GCAGCCGGCC GCGCTGCTCT TCAGTCTCCT GTGCGCCTTC 
TTCTGGATGG GCTTGTGCCT GCTGCGCGCC GGGGTGCGCC TGCCTCTGGC CGTCGCGCTG 
TTGGCCGCCT GCTGCGCCGG GGAAGCGCTG GTGCAGCTCT CCTTGGGCGT GGGGGACGGT 
CGCCTGCTGT CGCTGCCCGC CGCGGGGGTC CTGCTCAGCT GCCTAGGTGG CGCGACATGG 
CTGGTGCTGA GGCTGAGGCT GGGCGTCCTC ATGGTCGCCT GGACTAGCGT GCTTAGGACG 
GTGGCCCTGG TTTCTCTGGA GAGGTTCAAG GTCGCCTGGA GACCTTACCT GGCATACTTG 
GCGGCCGTGC TTGGGCTGCT GCTGGCCAGG TACGCCGAGC AGATCCTGCC GCAGTGCTCC 
GGGCCGGCTC CGCCCAGGGA GCGGTTTGGG TCCCAGTTGA GCGCGAGGAC CAAGGAAGAG 
ATTCCGGGGT GGAAGAGGAG GAGGCGGTCC AGCTCGGTGG TGGCCGGCGA GATGTCCGGC 
TGCAGTGGCA AGTCTCACCG GAGGACCTCC CTGCCCTGCA TACCCAGGGA GCAGCTCATG 
GGCCATTCAG AATGGGACCA CAAGAGAGGG CCACGAGGAT CCCAGTCAGG AACCAGCATC 
ACTGTGGATA TTGCTGTGAT GGGTGAGGCC CATGGCCTCA TTACCGACCT CCTGGCCGAC 
CCTTCTCTGC CCCCGAATGT GTGTACATCC TTGAGGGCCG TAAGCAACTT GCTCAGCACC 
CAGCTCACCT TCCAGGCCAT CCACAAGCCC AGAGTGAACC CCACCGTGAC CTTCAGTGAG 
AACTATACCT GCTCGGACTC TGAGGAAGGC TTGGAGAAAG ACAAGCAGGC CATTTCCAAG 
CGCCTGAGAA GAAGTTTGCC TCCAGGCTTA CTGAGAAGAG TCTCGTCAAC TTGGACAACT 
ACCACCTCTG CCACAGGTCT GCCCACCTTG GAGCCTGCGC CAGTGCGGAG GGACCGCAGT 
GCCAGCATAA AGCCGCACGA AGCCCCTTCA CCCAGTGCTG TCAATCCTGA CTCTTGGAAT 
GCCCCAGGGT TGACGACTCT CACCAAAAGC AGATCCTTCA CTTCATCCTA TGCTGTCTCT 
GCAGCTAACC ATGTAAAAGC TAAAAAGCAA AACAGGCCAG GTGGCCTTGC TAAAATTTCC 
CCCGTCCCCT CACCCTCCTC CTCGCCTCCC CAGGGATCAC CTGCCAGCAG TCCTGTCAGC 
AACAGTGCTT CACAACAGTT CCCAGAATCC CCTGAAGTAA CCATCAAGCG AGGGCCTGGA 
TCTCACAGGG CCTTAACTTA CACCCAAAGT GCCCCTGACC TGTCCCCTCA GATCCCACCC 
CCGTCTGTCA TATGTAGCAG CTGTGGCAGA CCTTATTCGC AAGGGAATCC TGCTGATGGA 
CCCTCAGAGA GAAGTGGCCC AGCCATGCTG AAACCAAACA GAACAGATGA CACTTCTCAA 
GTTACCTCCG ATTATGAGAC CAACAACAAC AGTGACAGCA GTGACATTCT GCAGAATGAA 
GAGGAAGCTG AGTGCCAGAG AGAACCCCAG AGGAAAGCAT CGGCTTGTGG CACCTATACT 
TCCCAGACCA TGATCTTCCT GGACAAACCA ATTCTTGCTC CAGAACCCCT GGTCATGGAT 
AACCTGGACT CAATTATGGA TCAGTTGAAC ACCTGGAATT TTCCAATTTT TGATTTAATG 
GAAAATATAG GAAGAAAATG TGGCCGTATT CTGAGCCAGG TATCATACAG ACTGTTTGAA 
GACATGGGGC TCTTTGAAGC CTTTAAAATC CCGGTTAGGG AGTTTATGAA TTACTTTCAT 
GCTTTGGAGA TCGGATACAG GGACATTCCT TATCATAACA GAATCCATGC CACCGATGTT 
CTACACGCTG TGTGGTATCT CACAACACAG CCGATTCCTG GCCTCCCAAG TGTGATTGGT 
GATCACGGCT CAGCAAGCGA CTCCGATTCT GACAGTGGAT TTACACACGG ACACATGGGA 
TATGTGTTTT CAAAAATGTA TCACGTGCCA GATGACAAAT ATGGATGCCT GTCTGGGAAT 
ATTCCAGCCC TAGAGTTGAT GGCTCTGTAC GTTGCTGCAG CCATGCATGA CTACGATCAC 
CCAGGGAGGA CAAATGCCTT CCTGGTTGCC ACTAGTGCCC CTCAGGCGGT GCTATACAAC 
GACCGTTCTG TTCTGGAGAA CCATCACGCC GCTGCAGCCT GGAATCTCTT CATGTCCCGG 
CCAGAGTATA ACTTCTTAGT TAACCTGGAC CACGTGGAAT TCAAGCACTT CCGCTTCCTA 
GTCATTGAAG CGATTTTGGC TACTGACCTG AAGAAACACT TTGACTTTGT AGCCAAATTT 
AACGCCAAGG TAAACGATGA TGTCGGGATA GATTGGACCA ATGAAAACGA CCGTCTCCTG 
GTGTGTCAGA TGTGTATAAA GCTGGCTGAC ATCAACGGGC CTGCTAAATG CAAAGAACTC 
CACCTGCGGT GGACAGAGGG CATCGCCAGC GAGTTTTATG AGCAGGGCGA TGAAGAAGCC 
AGCCTCGGCT TGCCCATAAG TCCCTTTATG GACCGCTCTG CCCCGCAGCT GGCCAATCTT 
CAGGAATCCT TTATCTCACA CATCGTGGGT CCTCTCTGCC ACTCCTATGA CTCTGCGGGA 
CTCATGCCAG GAAAATGGGT AGATGACAGT GATGATTCCG GAGACACCGA TGACCCGGAA 
GAAGAGGAGG AAGAGGCTGA AACACCACAC GAGGACGAAG CCTGTGAAAG CAGTATAGCT 
CCCCGAAAGA AAAGTTTCAA AAGAAGGAGA ATCTACTGCC AAATAACTCA GCACCTTCTG 
CAGAACCACA TGATGTGGAA GAAAGTGATT GAAGAGGAAC AGTGTTTATC AGGCACAGAG 
AACCAGTCCC TGGACCAGGT CCCTCTGCAG CATCCCTCAG AGCAGATCCA GGCTATCAAG 
GAGGAAGAGG AAGAGAAGGG GAAGCCAAGA GCAGAGGAGA CCCTGGCTCC ACAGCCAGAC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phosphodiesterase 3A expression and activity in the murine vasculature is influenced by NO-sensitive guanylyl cyclase.
Pflugers Archiv : European journal of physiology(2018 Jan)
D?nnes S,Voussen B,Aue A,Groneberg K,Nikolaev V,Groneberg D,Friebe A

Phosphodiesterase 3A: a new player in development of interstitial cells of Cajal and a prospective target in gastrointestinal stromal tumors (GIST).
Oncotarget8(25)41026-41043(2017 Jun)
Vandenberghe P,Hagu? P,Hockman SC,Manganiello VC,Demetter P,Erneux C,Vanderwinden JM

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Pde3a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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