Kalrn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kalrn
Entrez Gene ID	545156
Full Name	kalirin, RhoGEF kinase
Synonyms	2210407G14Rik,AV235988,DUET,E530005C20Rik,Gm539,Hapip,TRAD
General protein information	Preferred Names kalirin, RhoGEF kinase Names kalirin huntingtin-associated protein interacting protein (duo) kalrn protein Duo serine/threonine kinase with Dbl- and pleckstrin homology domain serine/threonine kinase with Dbl- and pleckstrin homology domains serine/threonine-protein kinase with Dbl- and pleckstrin homology domain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_011245954.2	XP_011244256.1	kalirin isoform X4
XM_011245955.1	XP_011244257.1	kalirin isoform X5
XM_017317071.1	XP_017172560.1	kalirin isoform X14
XM_011245963.2	XP_011244265.1	kalirin isoform X15
XM_011245966.2	XP_011244268.1	kalirin isoform X17
XM_011245968.2	XP_011244270.1	kalirin isoform X24
XM_017317075.1	XP_017172564.1	kalirin isoform X27
XM_006522386.3	XP_006522449.1	kalirin isoform X38
NM_001164268.1	NP_001157740.1	kalirin isoform 2
XM_011245954.3	XP_011244256.1	kalirin isoform X4
XM_011245961.3	XP_011244263.1	kalirin isoform X13
XM_017317071.2	XP_017172560.1	kalirin isoform X14
XM_011245965.3	XP_011244267.1	kalirin isoform X16
XM_011245966.3	XP_011244268.1	kalirin isoform X17
XM_017317074.2	XP_017172563.1	kalirin isoform X24
XM_006522385.4	XP_006522448.1	kalirin isoform X33
XM_006522364.3	XP_006522427.1	kalirin isoform X7
XM_006522370.3	XP_006522433.1	kalirin isoform X10
XM_011245961.2	XP_011244263.1	kalirin isoform X13
XM_017317072.1	XP_017172561.1	kalirin isoform X18
XM_017317073.1	XP_017172562.1	kalirin isoform X20
XM_011245970.2	XP_011244272.1	kalirin isoform X30
XM_011245953.3	XP_011244255.1	kalirin isoform X3
XM_006522370.4	XP_006522433.1	kalirin isoform X10
XM_011245958.2	XP_011244260.1	kalirin isoform X11
XM_011245963.3	XP_011244265.1	kalirin isoform X15
XM_011245967.3	XP_011244269.1	kalirin isoform X19
XM_006522380.1	XP_006522443.1	kalirin isoform X25
XM_006522383.4	XP_006522446.1	kalirin isoform X29
XM_017317076.2	XP_017172565.1	kalirin isoform X31
XM_017317082.2	XP_017172571.1	kalirin isoform X39
XM_011245951.2	XP_011244253.1	kalirin isoform X1
XM_011245957.2	XP_011244259.1	kalirin isoform X9
XM_011245967.2	XP_011244269.1	kalirin isoform X19
XM_006522382.3	XP_006522445.1	kalirin isoform X25
XM_011245969.2	XP_011244271.1	kalirin isoform X29
XM_006522385.3	XP_006522448.1	kalirin isoform X31
XM_017317077.1	XP_017172566.1	kalirin isoform X32
XM_017317078.1	XP_017172567.1	kalirin isoform X33
XM_017317079.1	XP_017172568.1	kalirin isoform X34
XM_011245951.3	XP_011244253.1	kalirin isoform X1
XM_011245952.3	XP_011244254.1	kalirin isoform X2
XM_011245955.2	XP_011244257.1	kalirin isoform X5
XM_030249190.1	XP_030105050.1	kalirin isoform X20
XM_030249192.1	XP_030105052.1	kalirin isoform X23
XM_006522381.1	XP_006522444.1	kalirin isoform X26
XM_006522382.4	XP_006522445.1	kalirin isoform X28
XM_017317075.2	XP_017172564.1	kalirin isoform X30
XM_017317077.2	XP_017172566.1	kalirin isoform X34
XM_017317080.2	XP_017172569.1	kalirin isoform X37
XM_017317081.2	XP_017172570.1	kalirin isoform X38
XM_011245952.2	XP_011244254.1	kalirin isoform X2
XM_011245953.2	XP_011244255.1	kalirin isoform X3
XM_006522363.3	XP_006522426.1	kalirin isoform X6
XM_011245956.2	XP_011244258.1	kalirin isoform X8
XM_011245960.2	XP_011244262.1	kalirin isoform X12
XM_006522363.4	XP_006522426.1	kalirin isoform X6
XM_011245959.2	XP_011244261.1	kalirin isoform X11
XM_011245960.3	XP_011244262.1	kalirin isoform X12
XM_017317073.2	XP_017172562.1	kalirin isoform X21
XM_011245970.3	XP_011244272.1	kalirin isoform X32
XM_017317078.2	XP_017172567.1	kalirin isoform X35
XM_017317079.2	XP_017172568.1	kalirin isoform X36
XM_006522386.4	XP_006522449.1	kalirin isoform X40
XM_011245965.2	XP_011244267.1	kalirin isoform X16
XM_017317074.1	XP_017172563.1	kalirin isoform X21
XM_006522383.3	XP_006522446.1	kalirin isoform X26
XM_017317076.1	XP_017172565.1	kalirin isoform X28
XM_017317080.1	XP_017172569.1	kalirin isoform X35
XM_017317081.1	XP_017172570.1	kalirin isoform X36
XM_017317082.1	XP_017172571.1	kalirin isoform X37
NM_177357.3	NP_796331.2	kalirin isoform 1
XM_006522364.4	XP_006522427.1	kalirin isoform X7
XM_011245956.3	XP_011244258.1	kalirin isoform X8
XM_011245957.3	XP_011244259.1	kalirin isoform X9
XM_017317072.2	XP_017172561.1	kalirin isoform X18
XM_030249191.1	XP_030105051.1	kalirin isoform X22
XM_011245968.3	XP_011244270.1	kalirin isoform X27

Pathways from BioSystems

Homologies

Homo sapiens (human)	KALRN	NP_001019831.2
	KALRN	XP_002802737.1
Gallus gallus (chicken)	KALRN	XP_004942990.1
Mus musculus (house mouse)	Kalrn	NP_796331.2
Rattus norvegicus (Norway rat)	Kalrn	NP_114451.2
Bos taurus (cattle)	KALRN	NP_001178293.1
Canis lupus familiaris (dog)	KALRN	XP_535768.3

Gene Ontology
Bibliography

Related articles in PubMed

Kalirin reduction rescues psychosis-associated behavioral deficits in APPswe/PSEN1dE9 transgenic mice.
Krivinko JM, Erickson SL, Abrahamson EE, Wills ZP, Ikonomovic MD, Penzes P, Sweet RA
Neurobiology of aging5459-70(2017 Jun)

Using Kalirin conditional knockout mice to distinguish its role in dopamine receptor mediated behaviors.
LaRese TP, Yan Y, Eipper BA, Mains RE
BMC neuroscience18(1)45(2017 May)

Synaptic pruning in the female hippocampus is triggered at puberty by extrasynaptic GABAA receptors on dendritic spines.
Afroz S, Parato J, Shen H, Smith SS
eLife5(2016 05)

A role for Kalirin-7 in nociceptive sensitization via activity-dependent modulation of spinal synapses.
Lu J, Luo C, Bali KK, Xie RG, Mains RE, Eipper BA, Kuner R
Nature communications66820(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kalirin reduction in APPswe/PSEN1dE9 mice significantly attenuated psychosis-associated behavior at 12 months of age without changing spatial memory performance.
Title: Kalirin reduction rescues psychosis-associated behavioral deficits in APPswe/PSEN1dE9 transgenic mice.

Kalirin-7 plays a prominent role in nociceptive sensitization via activity-dependent modulation of spinal synapses.
Title: A role for Kalirin-7 in nociceptive sensitization via activity-dependent modulation of spinal synapses.

lack of Kalrn expression in POMC cells closely mimicked the effects of global Kalrn knockout on anxiety-like behavior and passive avoidance conditioning
Title: Elimination of Kalrn expression in POMC cells reduces anxiety-like behavior and contextual fear learning.

Kal7 may allow coordination of the many signaling pathways contributing to dendritic spine morphogenesis.
Title: Nonenzymatic domains of Kalirin7 contribute to spine morphogenesis through interactions with phosphoinositides and Abl.

Kalirin may play a role in paracrine and/or endocrine signaling events that control skeletal bone remodeling and the maintenance of bone mass.
Title: The Rho-GEF Kalirin regulates bone mass and the function of osteoblasts and osteoclasts.

The following Kalrn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kalrn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu79376	XM_017317078.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu79377	XM_017317079.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu79378	XM_017317080.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu79379	XM_017317081.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X42, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu79380	XM_017317082.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X43, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu52301	XM_006522385.4 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu79375	XM_017317077.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu52298	XM_006522382.4 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52299	XM_006522383.4 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu75323	XM_011245968.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu75325	XM_011245970.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79373	XM_017317075.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79374	XM_017317076.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu21858	NM_001164268.1 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$972.30 $1389.00
OMu75324	XM_011245969.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu75318	XM_011245963.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu75320	XM_011245965.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu75321	XM_011245966.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu75322	XM_011245967.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu79370	XM_017317072.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu79371	XM_017317073.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu79372	XM_017317074.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu86978	XM_030249190.1 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu86979	XM_030249191.1 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu86980	XM_030249192.1 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu52296	XM_006522380.1 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu52297	XM_006522381.1 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu21881	NM_177357.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu52279	XM_006522363.4 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu52280	XM_006522364.4 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu52286	XM_006522370.4 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu52302	XM_006522386.4 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X44, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75307	XM_011245951.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75308	XM_011245952.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75309	XM_011245953.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75310	XM_011245954.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75311	XM_011245955.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75312	XM_011245956.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75313	XM_011245957.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75314	XM_011245958.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75314	XM_011245959.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75315	XM_011245960.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75316	XM_011245961.3 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu79369	XM_017317071.2 Latest version!	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OMu75310	XM_011245954.2	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu79369	XM_017317071.1	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75321	XM_011245966.2	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu75316	XM_011245961.2	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu52286	XM_006522370.3	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75318	XM_011245963.2	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu75322	XM_011245967.2	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu52301	XM_006522385.3	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu75307	XM_011245951.2	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75311	XM_011245955.1	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu52298	XM_006522382.3	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu79373	XM_017317075.1	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79375	XM_017317077.1	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu75308	XM_011245952.2	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75309	XM_011245953.2	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu52279	XM_006522363.3	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75315	XM_011245960.2	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu79371	XM_017317073.1	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu75325	XM_011245970.2	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu79376	XM_017317078.1	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu79377	XM_017317079.1	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu52302	XM_006522386.3	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75320	XM_011245965.2	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu79372	XM_017317074.1	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu52299	XM_006522383.3	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu79374	XM_017317076.1	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79378	XM_017317080.1	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu79379	XM_017317081.1	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu79380	XM_017317082.1	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu52280	XM_006522364.3	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75312	XM_011245956.2	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu75313	XM_011245957.2	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu79370	XM_017317072.1	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu75323	XM_011245968.2	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kalrn ( XM_017317078.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172567.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X36, mRNA.

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CloneID	OMu79376
Clone ID Related Accession (Same CDS sequence)	XM_017317078.1 , XM_017317078.2
Accession Version	XM_017317078.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GTCGTCTTTG 
CTAGCAGCCC GGCAGGCTCC CCCTGACGTG CCTACTGCTG CAGACCTTGT CAGTGCAATA 
GAAAAGTTGG TCAAAAATAA GCTGACTCTG GAAGGAGGCT CTTACCGGGG GAGCTTGAAG 
GACCCTACAG GCTGCCTGAA TGAGGGGATG ACCCCTCCCA CACCTCCTAG AAACCTGGAA 
GAAGAACAGA AAGCCAAGGC GCTGAGAGGC AGGATGTTTG TCCTGAATGA GCTGGTACAG 
ACAGAGAAGG ACTACGTCAA GGACCTGGGG ATTGTGGTGG AGGGCTTCAT GAAGAGAATA 
GAAGAGAAGG GGGTCCCTGA GGACATGCGA GGGAAGGAAA AGATCGTGTT TGGAAATATC 
CACCAGATTT ACGACTGGCA TAAAGATTTT TTCCTGGCTG AACTGGAAAA ATGTATCCAA 
GAGCAAGACA GATTGGCCCA GCTCTTCATT AAACACGAGC GGAAGCTGCA CATCTACGTG 
TGGTACTGCC AGAACAAGCC GCGTTCCGAG TACATCGTGG CTGAGTACGA TGCCTATTTT 
GAGGAGGTTA AACAAGAGAT CAATCAAAGG CTGACCCTTA GTGACTTCCT GATCAAGCCC 
ATTCAGAGAA TAACAAAGTA CCAGCTGCTA CTCAAGGACT TCCTGCGATA CAGCGAGAAG 
GCTGGTTTGG AGTGTTCCGA TATTGAGAAA GCAGTGGAGT TAATGTGCCT TGTCCCCAAG 
CGCTGTAATG ACATGATGAA TCTGGGACGC CTGCAGGGCT TCGAGGGCAC CCTGACCGCC 
CAGGGCAAGC TACTGCAGCA GGACACATTC TATGTGATCG AGCTGGATGC CGGCATGCAG 
TCCCGGACCA AGGAGAGGCG TGTGTTTCTC TTTGAGCAGA TTGTCATCTT CAGTGAACTG 
CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC ATGTTCAAAA GAAGCATCAA GATGAATTAC 
TTGGTCCTGG AAGACAATGT GGATGGTGAC CCCTGCAAAT TTGCACTCAT GAACAGGGAG 
ACTTCAGAAA GGGTCATTCT ACAGGCTGCC AACTCAGACA TCCAGCAGGC CTGGGTGCAG 
GACATCAACC AAGTCCTAGA AACACAGAGG GACTTCTTAA ATGCACTACA GTCACCCATT 
GAGTATCAGC GGAAGGAGAG AAGCACAGCT GTGATCCGGT CCCAGCCCCC GAGGGTTCCA 
CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC 
TCCAGCTATA ACCCGCCTCT GCCACCCCTG AAGATATCTA CCTCTAATGG CAGTCCAGGG 
TTCGACTACC ATCAGCCTGG GGACAAGTTT GACGCTAGCA AGAACGACCT GGGAGGCTGC 
AATGGGACAT CAACCATGAC TGTAATCAAA GATTACTATG CACTGAAGGA GAATGAAATC 
TGCGTGAGCC AAGGTGAGGT GGTACAGGTC CTCGCCGTCA ACCAGCAGAA CATGTGTCTG 
GTGTACCAGC CTGCCAGCGA CCATTCCCCG GCAGCAGAGG GCTGGGTCCC AGGCAGCATC 
CTGGCGCCCC TCGCCAAAGC CACAGCAGCG GCCGAAAGTA GTGACGGGAG CATCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172567.1
CDS	1578..3677
Translation	MKGGDQAYTRGPSLGWLFAKCCCCFPCRDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIK

Target ORF information:

RefSeq Version	XM_017317078.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317078.1

ORF Insert Sequence:

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GTCGTCTTTG 
CTAGCAGCCC GGCAGGCTCC CCCTGACGTG CCTACTGCTG CAGACCTTGT CAGTGCAATA 
GAAAAGTTGG TCAAAAATAA GCTGACTCTG GAAGGAGGCT CTTACCGGGG GAGCTTGAAG 
GACCCTACAG GCTGCCTGAA TGAGGGGATG ACCCCTCCCA CACCTCCTAG AAACCTGGAA 
GAAGAACAGA AAGCCAAGGC GCTGAGAGGC AGGATGTTTG TCCTGAATGA GCTGGTACAG 
ACAGAGAAGG ACTACGTCAA GGACCTGGGG ATTGTGGTGG AGGGCTTCAT GAAGAGAATA 
GAAGAGAAGG GGGTCCCTGA GGACATGCGA GGGAAGGAAA AGATCGTGTT TGGAAATATC 
CACCAGATTT ACGACTGGCA TAAAGATTTT TTCCTGGCTG AACTGGAAAA ATGTATCCAA 
GAGCAAGACA GATTGGCCCA GCTCTTCATT AAACACGAGC GGAAGCTGCA CATCTACGTG 
TGGTACTGCC AGAACAAGCC GCGTTCCGAG TACATCGTGG CTGAGTACGA TGCCTATTTT 
GAGGAGGTTA AACAAGAGAT CAATCAAAGG CTGACCCTTA GTGACTTCCT GATCAAGCCC 
ATTCAGAGAA TAACAAAGTA CCAGCTGCTA CTCAAGGACT TCCTGCGATA CAGCGAGAAG 
GCTGGTTTGG AGTGTTCCGA TATTGAGAAA GCAGTGGAGT TAATGTGCCT TGTCCCCAAG 
CGCTGTAATG ACATGATGAA TCTGGGACGC CTGCAGGGCT TCGAGGGCAC CCTGACCGCC 
CAGGGCAAGC TACTGCAGCA GGACACATTC TATGTGATCG AGCTGGATGC CGGCATGCAG 
TCCCGGACCA AGGAGAGGCG TGTGTTTCTC TTTGAGCAGA TTGTCATCTT CAGTGAACTG 
CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC ATGTTCAAAA GAAGCATCAA GATGAATTAC 
TTGGTCCTGG AAGACAATGT GGATGGTGAC CCCTGCAAAT TTGCACTCAT GAACAGGGAG 
ACTTCAGAAA GGGTCATTCT ACAGGCTGCC AACTCAGACA TCCAGCAGGC CTGGGTGCAG 
GACATCAACC AAGTCCTAGA AACACAGAGG GACTTCTTAA ATGCACTACA GTCACCCATT 
GAGTATCAGC GGAAGGAGAG AAGCACAGCT GTGATCCGGT CCCAGCCCCC GAGGGTTCCA 
CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC 
TCCAGCTATA ACCCGCCTCT GCCACCCCTG AAGATATCTA CCTCTAATGG CAGTCCAGGG 
TTCGACTACC ATCAGCCTGG GGACAAGTTT GACGCTAGCA AGAACGACCT GGGAGGCTGC 
AATGGGACAT CAACCATGAC TGTAATCAAA GATTACTATG CACTGAAGGA GAATGAAATC 
TGCGTGAGCC AAGGTGAGGT GGTACAGGTC CTCGCCGTCA ACCAGCAGAA CATGTGTCTG 
GTGTACCAGC CTGCCAGCGA CCATTCCCCG GCAGCAGAGG GCTGGGTCCC AGGCAGCATC 
CTGGCGCCCC TCGCCAAAGC CACAGCAGCG GCCGAAAGTA GTGACGGGAG CATCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317078.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172567.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X39, mRNA.

Price & Availability↑

CloneID	OMu79376
Clone ID Related Accession (Same CDS sequence)	XM_017317078.1 , XM_017317078.2
Accession Version	XM_017317078.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317078.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GTCGTCTTTG 
CTAGCAGCCC GGCAGGCTCC CCCTGACGTG CCTACTGCTG CAGACCTTGT CAGTGCAATA 
GAAAAGTTGG TCAAAAATAA GCTGACTCTG GAAGGAGGCT CTTACCGGGG GAGCTTGAAG 
GACCCTACAG GCTGCCTGAA TGAGGGGATG ACCCCTCCCA CACCTCCTAG AAACCTGGAA 
GAAGAACAGA AAGCCAAGGC GCTGAGAGGC AGGATGTTTG TCCTGAATGA GCTGGTACAG 
ACAGAGAAGG ACTACGTCAA GGACCTGGGG ATTGTGGTGG AGGGCTTCAT GAAGAGAATA 
GAAGAGAAGG GGGTCCCTGA GGACATGCGA GGGAAGGAAA AGATCGTGTT TGGAAATATC 
CACCAGATTT ACGACTGGCA TAAAGATTTT TTCCTGGCTG AACTGGAAAA ATGTATCCAA 
GAGCAAGACA GATTGGCCCA GCTCTTCATT AAACACGAGC GGAAGCTGCA CATCTACGTG 
TGGTACTGCC AGAACAAGCC GCGTTCCGAG TACATCGTGG CTGAGTACGA TGCCTATTTT 
GAGGAGGTTA AACAAGAGAT CAATCAAAGG CTGACCCTTA GTGACTTCCT GATCAAGCCC 
ATTCAGAGAA TAACAAAGTA CCAGCTGCTA CTCAAGGACT TCCTGCGATA CAGCGAGAAG 
GCTGGTTTGG AGTGTTCCGA TATTGAGAAA GCAGTGGAGT TAATGTGCCT TGTCCCCAAG 
CGCTGTAATG ACATGATGAA TCTGGGACGC CTGCAGGGCT TCGAGGGCAC CCTGACCGCC 
CAGGGCAAGC TACTGCAGCA GGACACATTC TATGTGATCG AGCTGGATGC CGGCATGCAG 
TCCCGGACCA AGGAGAGGCG TGTGTTTCTC TTTGAGCAGA TTGTCATCTT CAGTGAACTG 
CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC ATGTTCAAAA GAAGCATCAA GATGAATTAC 
TTGGTCCTGG AAGACAATGT GGATGGTGAC CCCTGCAAAT TTGCACTCAT GAACAGGGAG 
ACTTCAGAAA GGGTCATTCT ACAGGCTGCC AACTCAGACA TCCAGCAGGC CTGGGTGCAG 
GACATCAACC AAGTCCTAGA AACACAGAGG GACTTCTTAA ATGCACTACA GTCACCCATT 
GAGTATCAGC GGAAGGAGAG AAGCACAGCT GTGATCCGGT CCCAGCCCCC GAGGGTTCCA 
CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC 
TCCAGCTATA ACCCGCCTCT GCCACCCCTG AAGATATCTA CCTCTAATGG CAGTCCAGGG 
TTCGACTACC ATCAGCCTGG GGACAAGTTT GACGCTAGCA AGAACGACCT GGGAGGCTGC 
AATGGGACAT CAACCATGAC TGTAATCAAA GATTACTATG CACTGAAGGA GAATGAAATC 
TGCGTGAGCC AAGGTGAGGT GGTACAGGTC CTCGCCGTCA ACCAGCAGAA CATGTGTCTG 
GTGTACCAGC CTGCCAGCGA CCATTCCCCG GCAGCAGAGG GCTGGGTCCC AGGCAGCATC 
CTGGCGCCCC TCGCCAAAGC CACAGCAGCG GCCGAAAGTA GTGACGGGAG CATCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172567.1
CDS	1521..3620
Translation	MKGGDQAYTRGPSLGWLFAKCCCCFPCRDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIK

Target ORF information:

RefSeq Version	XM_017317078.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317078.2

ORF Insert Sequence:

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GTCGTCTTTG 
CTAGCAGCCC GGCAGGCTCC CCCTGACGTG CCTACTGCTG CAGACCTTGT CAGTGCAATA 
GAAAAGTTGG TCAAAAATAA GCTGACTCTG GAAGGAGGCT CTTACCGGGG GAGCTTGAAG 
GACCCTACAG GCTGCCTGAA TGAGGGGATG ACCCCTCCCA CACCTCCTAG AAACCTGGAA 
GAAGAACAGA AAGCCAAGGC GCTGAGAGGC AGGATGTTTG TCCTGAATGA GCTGGTACAG 
ACAGAGAAGG ACTACGTCAA GGACCTGGGG ATTGTGGTGG AGGGCTTCAT GAAGAGAATA 
GAAGAGAAGG GGGTCCCTGA GGACATGCGA GGGAAGGAAA AGATCGTGTT TGGAAATATC 
CACCAGATTT ACGACTGGCA TAAAGATTTT TTCCTGGCTG AACTGGAAAA ATGTATCCAA 
GAGCAAGACA GATTGGCCCA GCTCTTCATT AAACACGAGC GGAAGCTGCA CATCTACGTG 
TGGTACTGCC AGAACAAGCC GCGTTCCGAG TACATCGTGG CTGAGTACGA TGCCTATTTT 
GAGGAGGTTA AACAAGAGAT CAATCAAAGG CTGACCCTTA GTGACTTCCT GATCAAGCCC 
ATTCAGAGAA TAACAAAGTA CCAGCTGCTA CTCAAGGACT TCCTGCGATA CAGCGAGAAG 
GCTGGTTTGG AGTGTTCCGA TATTGAGAAA GCAGTGGAGT TAATGTGCCT TGTCCCCAAG 
CGCTGTAATG ACATGATGAA TCTGGGACGC CTGCAGGGCT TCGAGGGCAC CCTGACCGCC 
CAGGGCAAGC TACTGCAGCA GGACACATTC TATGTGATCG AGCTGGATGC CGGCATGCAG 
TCCCGGACCA AGGAGAGGCG TGTGTTTCTC TTTGAGCAGA TTGTCATCTT CAGTGAACTG 
CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC ATGTTCAAAA GAAGCATCAA GATGAATTAC 
TTGGTCCTGG AAGACAATGT GGATGGTGAC CCCTGCAAAT TTGCACTCAT GAACAGGGAG 
ACTTCAGAAA GGGTCATTCT ACAGGCTGCC AACTCAGACA TCCAGCAGGC CTGGGTGCAG 
GACATCAACC AAGTCCTAGA AACACAGAGG GACTTCTTAA ATGCACTACA GTCACCCATT 
GAGTATCAGC GGAAGGAGAG AAGCACAGCT GTGATCCGGT CCCAGCCCCC GAGGGTTCCA 
CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC 
TCCAGCTATA ACCCGCCTCT GCCACCCCTG AAGATATCTA CCTCTAATGG CAGTCCAGGG 
TTCGACTACC ATCAGCCTGG GGACAAGTTT GACGCTAGCA AGAACGACCT GGGAGGCTGC 
AATGGGACAT CAACCATGAC TGTAATCAAA GATTACTATG CACTGAAGGA GAATGAAATC 
TGCGTGAGCC AAGGTGAGGT GGTACAGGTC CTCGCCGTCA ACCAGCAGAA CATGTGTCTG 
GTGTACCAGC CTGCCAGCGA CCATTCCCCG GCAGCAGAGG GCTGGGTCCC AGGCAGCATC 
CTGGCGCCCC TCGCCAAAGC CACAGCAGCG GCCGAAAGTA GTGACGGGAG CATCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317079.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172568.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X37, mRNA.

Price & Availability↑

CloneID	OMu79377
Clone ID Related Accession (Same CDS sequence)	XM_017317079.1 , XM_017317079.2
Accession Version	XM_017317079.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGACTCTG 
GAAGGAGGCT CTTACCGGGG GAGCTTGAAG GACCCTACAG GCTGCCTGAA TGAGGGGATG 
ACCCCTCCCA CACCTCCTAG AAACCTGGAA GAAGAACAGA AAGCCAAGGC GCTGAGAGGC 
AGGATGTTTG TCCTGAATGA GCTGGTACAG ACAGAGAAGG ACTACGTCAA GGACCTGGGG 
ATTGTGGTGG AGGGCTTCAT GAAGAGAATA GAAGAGAAGG GGGTCCCTGA GGACATGCGA 
GGGAAGGAAA AGATCGTGTT TGGAAATATC CACCAGATTT ACGACTGGCA TAAAGATTTT 
TTCCTGGCTG AACTGGAAAA ATGTATCCAA GAGCAAGACA GATTGGCCCA GCTCTTCATT 
AAACACGAGC GGAAGCTGCA CATCTACGTG TGGTACTGCC AGAACAAGCC GCGTTCCGAG 
TACATCGTGG CTGAGTACGA TGCCTATTTT GAGGAGGTTA AACAAGAGAT CAATCAAAGG 
CTGACCCTTA GTGACTTCCT GATCAAGCCC ATTCAGAGAA TAACAAAGTA CCAGCTGCTA 
CTCAAGGACT TCCTGCGATA CAGCGAGAAG GCTGGTTTGG AGTGTTCCGA TATTGAGAAA 
GCAGTGGAGT TAATGTGCCT TGTCCCCAAG CGCTGTAATG ACATGATGAA TCTGGGACGC 
CTGCAGGGCT TCGAGGGCAC CCTGACCGCC CAGGGCAAGC TACTGCAGCA GGACACATTC 
TATGTGATCG AGCTGGATGC CGGCATGCAG TCCCGGACCA AGGAGAGGCG TGTGTTTCTC 
TTTGAGCAGA TTGTCATCTT CAGTGAACTG CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC 
ATGTTCAAAA GAAGCATCAA GATGAATTAC TTGGTCCTGG AAGACAATGT GGATGGTGAC 
CCCTGCAAAT TTGCACTCAT GAACAGGGAG ACTTCAGAAA GGGTCATTCT ACAGGCTGCC 
AACTCAGACA TCCAGCAGGC CTGGGTGCAG GACATCAACC AAGTCCTAGA AACACAGAGG 
GACTTCTTAA ATGCACTACA GTCACCCATT GAGTATCAGC GGAAGGAGAG AAGCACAGCT 
GTGATCCGGT CCCAGCCCCC GAGGGTTCCA CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC 
CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC TCCAGCTATA ACCCGCCTCT GCCACCCCTG 
AAGATATCTA CCTCTAATGG CAGTCCAGGG TTCGACTACC ATCAGCCTGG GGACAAGTTT 
GACGCTAGCA AGCAGAACGA CCTGGGAGGC TGCAATGGGA CATCAACCAT GACTGTAATC 
AAAGATTACT ATGCACTGAA GGAGAATGAA ATCTGCGTGA GCCAAGGTGA GGTGGTACAG 
GTCCTCGCCG TCAACCAGCA GAACATGTGT CTGGTGTACC AGCCTGCCAG CGACCATTCC 
CCGGCAGCAG AGGGCTGGGT CCCAGGCAGC ATCCTGGCGC CCCTCGCCAA AGCCACAGCA 
GCGGCCGAAA GTAGTGACGG GAGCATCAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172568.1
CDS	1578..3590
Translation	MKGGDQAYTRGPSLGWLFAKCCCCFPCRDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIK

Target ORF information:

RefSeq Version	XM_017317079.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317079.1

ORF Insert Sequence:

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGACTCTG 
GAAGGAGGCT CTTACCGGGG GAGCTTGAAG GACCCTACAG GCTGCCTGAA TGAGGGGATG 
ACCCCTCCCA CACCTCCTAG AAACCTGGAA GAAGAACAGA AAGCCAAGGC GCTGAGAGGC 
AGGATGTTTG TCCTGAATGA GCTGGTACAG ACAGAGAAGG ACTACGTCAA GGACCTGGGG 
ATTGTGGTGG AGGGCTTCAT GAAGAGAATA GAAGAGAAGG GGGTCCCTGA GGACATGCGA 
GGGAAGGAAA AGATCGTGTT TGGAAATATC CACCAGATTT ACGACTGGCA TAAAGATTTT 
TTCCTGGCTG AACTGGAAAA ATGTATCCAA GAGCAAGACA GATTGGCCCA GCTCTTCATT 
AAACACGAGC GGAAGCTGCA CATCTACGTG TGGTACTGCC AGAACAAGCC GCGTTCCGAG 
TACATCGTGG CTGAGTACGA TGCCTATTTT GAGGAGGTTA AACAAGAGAT CAATCAAAGG 
CTGACCCTTA GTGACTTCCT GATCAAGCCC ATTCAGAGAA TAACAAAGTA CCAGCTGCTA 
CTCAAGGACT TCCTGCGATA CAGCGAGAAG GCTGGTTTGG AGTGTTCCGA TATTGAGAAA 
GCAGTGGAGT TAATGTGCCT TGTCCCCAAG CGCTGTAATG ACATGATGAA TCTGGGACGC 
CTGCAGGGCT TCGAGGGCAC CCTGACCGCC CAGGGCAAGC TACTGCAGCA GGACACATTC 
TATGTGATCG AGCTGGATGC CGGCATGCAG TCCCGGACCA AGGAGAGGCG TGTGTTTCTC 
TTTGAGCAGA TTGTCATCTT CAGTGAACTG CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC 
ATGTTCAAAA GAAGCATCAA GATGAATTAC TTGGTCCTGG AAGACAATGT GGATGGTGAC 
CCCTGCAAAT TTGCACTCAT GAACAGGGAG ACTTCAGAAA GGGTCATTCT ACAGGCTGCC 
AACTCAGACA TCCAGCAGGC CTGGGTGCAG GACATCAACC AAGTCCTAGA AACACAGAGG 
GACTTCTTAA ATGCACTACA GTCACCCATT GAGTATCAGC GGAAGGAGAG AAGCACAGCT 
GTGATCCGGT CCCAGCCCCC GAGGGTTCCA CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC 
CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC TCCAGCTATA ACCCGCCTCT GCCACCCCTG 
AAGATATCTA CCTCTAATGG CAGTCCAGGG TTCGACTACC ATCAGCCTGG GGACAAGTTT 
GACGCTAGCA AGCAGAACGA CCTGGGAGGC TGCAATGGGA CATCAACCAT GACTGTAATC 
AAAGATTACT ATGCACTGAA GGAGAATGAA ATCTGCGTGA GCCAAGGTGA GGTGGTACAG 
GTCCTCGCCG TCAACCAGCA GAACATGTGT CTGGTGTACC AGCCTGCCAG CGACCATTCC 
CCGGCAGCAG AGGGCTGGGT CCCAGGCAGC ATCCTGGCGC CCCTCGCCAA AGCCACAGCA 
GCGGCCGAAA GTAGTGACGG GAGCATCAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317079.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172568.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X40, mRNA.

Price & Availability↑

CloneID	OMu79377
Clone ID Related Accession (Same CDS sequence)	XM_017317079.1 , XM_017317079.2
Accession Version	XM_017317079.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317079.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGACTCTG 
GAAGGAGGCT CTTACCGGGG GAGCTTGAAG GACCCTACAG GCTGCCTGAA TGAGGGGATG 
ACCCCTCCCA CACCTCCTAG AAACCTGGAA GAAGAACAGA AAGCCAAGGC GCTGAGAGGC 
AGGATGTTTG TCCTGAATGA GCTGGTACAG ACAGAGAAGG ACTACGTCAA GGACCTGGGG 
ATTGTGGTGG AGGGCTTCAT GAAGAGAATA GAAGAGAAGG GGGTCCCTGA GGACATGCGA 
GGGAAGGAAA AGATCGTGTT TGGAAATATC CACCAGATTT ACGACTGGCA TAAAGATTTT 
TTCCTGGCTG AACTGGAAAA ATGTATCCAA GAGCAAGACA GATTGGCCCA GCTCTTCATT 
AAACACGAGC GGAAGCTGCA CATCTACGTG TGGTACTGCC AGAACAAGCC GCGTTCCGAG 
TACATCGTGG CTGAGTACGA TGCCTATTTT GAGGAGGTTA AACAAGAGAT CAATCAAAGG 
CTGACCCTTA GTGACTTCCT GATCAAGCCC ATTCAGAGAA TAACAAAGTA CCAGCTGCTA 
CTCAAGGACT TCCTGCGATA CAGCGAGAAG GCTGGTTTGG AGTGTTCCGA TATTGAGAAA 
GCAGTGGAGT TAATGTGCCT TGTCCCCAAG CGCTGTAATG ACATGATGAA TCTGGGACGC 
CTGCAGGGCT TCGAGGGCAC CCTGACCGCC CAGGGCAAGC TACTGCAGCA GGACACATTC 
TATGTGATCG AGCTGGATGC CGGCATGCAG TCCCGGACCA AGGAGAGGCG TGTGTTTCTC 
TTTGAGCAGA TTGTCATCTT CAGTGAACTG CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC 
ATGTTCAAAA GAAGCATCAA GATGAATTAC TTGGTCCTGG AAGACAATGT GGATGGTGAC 
CCCTGCAAAT TTGCACTCAT GAACAGGGAG ACTTCAGAAA GGGTCATTCT ACAGGCTGCC 
AACTCAGACA TCCAGCAGGC CTGGGTGCAG GACATCAACC AAGTCCTAGA AACACAGAGG 
GACTTCTTAA ATGCACTACA GTCACCCATT GAGTATCAGC GGAAGGAGAG AAGCACAGCT 
GTGATCCGGT CCCAGCCCCC GAGGGTTCCA CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC 
CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC TCCAGCTATA ACCCGCCTCT GCCACCCCTG 
AAGATATCTA CCTCTAATGG CAGTCCAGGG TTCGACTACC ATCAGCCTGG GGACAAGTTT 
GACGCTAGCA AGCAGAACGA CCTGGGAGGC TGCAATGGGA CATCAACCAT GACTGTAATC 
AAAGATTACT ATGCACTGAA GGAGAATGAA ATCTGCGTGA GCCAAGGTGA GGTGGTACAG 
GTCCTCGCCG TCAACCAGCA GAACATGTGT CTGGTGTACC AGCCTGCCAG CGACCATTCC 
CCGGCAGCAG AGGGCTGGGT CCCAGGCAGC ATCCTGGCGC CCCTCGCCAA AGCCACAGCA 
GCGGCCGAAA GTAGTGACGG GAGCATCAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172568.1
CDS	1521..3533
Translation	MKGGDQAYTRGPSLGWLFAKCCCCFPCRDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIK

Target ORF information:

RefSeq Version	XM_017317079.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317079.2

ORF Insert Sequence:

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGACTCTG 
GAAGGAGGCT CTTACCGGGG GAGCTTGAAG GACCCTACAG GCTGCCTGAA TGAGGGGATG 
ACCCCTCCCA CACCTCCTAG AAACCTGGAA GAAGAACAGA AAGCCAAGGC GCTGAGAGGC 
AGGATGTTTG TCCTGAATGA GCTGGTACAG ACAGAGAAGG ACTACGTCAA GGACCTGGGG 
ATTGTGGTGG AGGGCTTCAT GAAGAGAATA GAAGAGAAGG GGGTCCCTGA GGACATGCGA 
GGGAAGGAAA AGATCGTGTT TGGAAATATC CACCAGATTT ACGACTGGCA TAAAGATTTT 
TTCCTGGCTG AACTGGAAAA ATGTATCCAA GAGCAAGACA GATTGGCCCA GCTCTTCATT 
AAACACGAGC GGAAGCTGCA CATCTACGTG TGGTACTGCC AGAACAAGCC GCGTTCCGAG 
TACATCGTGG CTGAGTACGA TGCCTATTTT GAGGAGGTTA AACAAGAGAT CAATCAAAGG 
CTGACCCTTA GTGACTTCCT GATCAAGCCC ATTCAGAGAA TAACAAAGTA CCAGCTGCTA 
CTCAAGGACT TCCTGCGATA CAGCGAGAAG GCTGGTTTGG AGTGTTCCGA TATTGAGAAA 
GCAGTGGAGT TAATGTGCCT TGTCCCCAAG CGCTGTAATG ACATGATGAA TCTGGGACGC 
CTGCAGGGCT TCGAGGGCAC CCTGACCGCC CAGGGCAAGC TACTGCAGCA GGACACATTC 
TATGTGATCG AGCTGGATGC CGGCATGCAG TCCCGGACCA AGGAGAGGCG TGTGTTTCTC 
TTTGAGCAGA TTGTCATCTT CAGTGAACTG CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC 
ATGTTCAAAA GAAGCATCAA GATGAATTAC TTGGTCCTGG AAGACAATGT GGATGGTGAC 
CCCTGCAAAT TTGCACTCAT GAACAGGGAG ACTTCAGAAA GGGTCATTCT ACAGGCTGCC 
AACTCAGACA TCCAGCAGGC CTGGGTGCAG GACATCAACC AAGTCCTAGA AACACAGAGG 
GACTTCTTAA ATGCACTACA GTCACCCATT GAGTATCAGC GGAAGGAGAG AAGCACAGCT 
GTGATCCGGT CCCAGCCCCC GAGGGTTCCA CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC 
CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC TCCAGCTATA ACCCGCCTCT GCCACCCCTG 
AAGATATCTA CCTCTAATGG CAGTCCAGGG TTCGACTACC ATCAGCCTGG GGACAAGTTT 
GACGCTAGCA AGCAGAACGA CCTGGGAGGC TGCAATGGGA CATCAACCAT GACTGTAATC 
AAAGATTACT ATGCACTGAA GGAGAATGAA ATCTGCGTGA GCCAAGGTGA GGTGGTACAG 
GTCCTCGCCG TCAACCAGCA GAACATGTGT CTGGTGTACC AGCCTGCCAG CGACCATTCC 
CCGGCAGCAG AGGGCTGGGT CCCAGGCAGC ATCCTGGCGC CCCTCGCCAA AGCCACAGCA 
GCGGCCGAAA GTAGTGACGG GAGCATCAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317080.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172569.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X41, mRNA.

Price & Availability↑

CloneID	OMu79378
Clone ID Related Accession (Same CDS sequence)	XM_017317080.2 , XM_017317080.1
Accession Version	XM_017317080.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X37
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GACTCTGGAA 
GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC CCTACAGGCT GCCTGAATGA GGGGATGACC 
CCTCCCACAC CTCCTAGAAA CCTGGAAGAA GAACAGAAAG CCAAGGCGCT GAGAGGCAGG 
ATGTTTGTCC TGAATGAGCT GGTACAGACA GAGAAGGACT ACGTCAAGGA CCTGGGGATT 
GTGGTGGAGG GCTTCATGAA GAGAATAGAA GAGAAGGGGG TCCCTGAGGA CATGCGAGGG 
AAGGAAAAGA TCGTGTTTGG AAATATCCAC CAGATTTACG ACTGGCATAA AGATTTTTTC 
CTGGCTGAAC TGGAAAAATG TATCCAAGAG CAAGACAGAT TGGCCCAGCT CTTCATTAAA 
CACGAGCGGA AGCTGCACAT CTACGTGTGG TACTGCCAGA ACAAGCCGCG TTCCGAGTAC 
ATCGTGGCTG AGTACGATGC CTATTTTGAG GAGGTTAAAC AAGAGATCAA TCAAAGGCTG 
ACCCTTAGTG ACTTCCTGAT CAAGCCCATT CAGAGAATAA CAAAGTACCA GCTGCTACTC 
AAGGACTTCC TGCGATACAG CGAGAAGGCT GGTTTGGAGT GTTCCGATAT TGAGAAAGCA 
GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC TGTAATGACA TGATGAATCT GGGACGCCTG 
CAGGGCTTCG AGGGCACCCT GACCGCCCAG GGCAAGCTAC TGCAGCAGGA CACATTCTAT 
GTGATCGAGC TGGATGCCGG CATGCAGTCC CGGACCAAGG AGAGGCGTGT GTTTCTCTTT 
GAGCAGATTG TCATCTTCAG TGAACTGCTC CGAAAGGGGT CCCTCACCCC AGGCTACATG 
TTCAAAAGAA GCATCAAGAT GAATTACTTG GTCCTGGAAG ACAATGTGGA TGGTGACCCC 
TGCAAATTTG CACTCATGAA CAGGGAGACT TCAGAAAGGG TCATTCTACA GGCTGCCAAC 
TCAGACATCC AGCAGGCCTG GGTGCAGGAC ATCAACCAAG TCCTAGAAAC ACAGAGGGAC 
TTCTTAAATG CACTACAGTC ACCCATTGAG TATCAGCGGA AGGAGAGAAG CACAGCTGTG 
ATCCGGTCCC AGCCCCCGAG GGTTCCACAA GCCAGCCCCA GGCCCTACTC TTCTGGCCCG 
GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC AGCTATAACC CGCCTCTGCC ACCCCTGAAG 
ATATCTACCT CTAATGGCAG TCCAGGGTTC GACTACCATC AGCCTGGGGA CAAGTTTGAC 
GCTAGCAAGC AGAACGACCT GGGAGGCTGC AATGGGACAT CAACCATGAC TGTAATCAAA 
GATTACTATG CACTGAAGGA GAATGAAATC TGCGTGAGCC AAGGTGAGGT GGTACAGGTC 
CTCGCCGTCA ACCAGCAGAA CATGTGTCTG GTGTACCAGC CTGCCAGCGA CCATTCCCCG 
GCAGCAGAGG GCTGGGTCCC AGGCAGCATC CTGGCGCCCC TCGCCAAAGC CACAGCAGCG 
GCCGAAAGTA GTGACGGGAG CATCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172569.1
CDS	1521..3530
Translation	MKGGDQAYTRGPSLGWLFAKCCCCFPCRDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIK

Target ORF information:

RefSeq Version	XM_017317080.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317080.2

ORF Insert Sequence:

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GACTCTGGAA 
GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC CCTACAGGCT GCCTGAATGA GGGGATGACC 
CCTCCCACAC CTCCTAGAAA CCTGGAAGAA GAACAGAAAG CCAAGGCGCT GAGAGGCAGG 
ATGTTTGTCC TGAATGAGCT GGTACAGACA GAGAAGGACT ACGTCAAGGA CCTGGGGATT 
GTGGTGGAGG GCTTCATGAA GAGAATAGAA GAGAAGGGGG TCCCTGAGGA CATGCGAGGG 
AAGGAAAAGA TCGTGTTTGG AAATATCCAC CAGATTTACG ACTGGCATAA AGATTTTTTC 
CTGGCTGAAC TGGAAAAATG TATCCAAGAG CAAGACAGAT TGGCCCAGCT CTTCATTAAA 
CACGAGCGGA AGCTGCACAT CTACGTGTGG TACTGCCAGA ACAAGCCGCG TTCCGAGTAC 
ATCGTGGCTG AGTACGATGC CTATTTTGAG GAGGTTAAAC AAGAGATCAA TCAAAGGCTG 
ACCCTTAGTG ACTTCCTGAT CAAGCCCATT CAGAGAATAA CAAAGTACCA GCTGCTACTC 
AAGGACTTCC TGCGATACAG CGAGAAGGCT GGTTTGGAGT GTTCCGATAT TGAGAAAGCA 
GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC TGTAATGACA TGATGAATCT GGGACGCCTG 
CAGGGCTTCG AGGGCACCCT GACCGCCCAG GGCAAGCTAC TGCAGCAGGA CACATTCTAT 
GTGATCGAGC TGGATGCCGG CATGCAGTCC CGGACCAAGG AGAGGCGTGT GTTTCTCTTT 
GAGCAGATTG TCATCTTCAG TGAACTGCTC CGAAAGGGGT CCCTCACCCC AGGCTACATG 
TTCAAAAGAA GCATCAAGAT GAATTACTTG GTCCTGGAAG ACAATGTGGA TGGTGACCCC 
TGCAAATTTG CACTCATGAA CAGGGAGACT TCAGAAAGGG TCATTCTACA GGCTGCCAAC 
TCAGACATCC AGCAGGCCTG GGTGCAGGAC ATCAACCAAG TCCTAGAAAC ACAGAGGGAC 
TTCTTAAATG CACTACAGTC ACCCATTGAG TATCAGCGGA AGGAGAGAAG CACAGCTGTG 
ATCCGGTCCC AGCCCCCGAG GGTTCCACAA GCCAGCCCCA GGCCCTACTC TTCTGGCCCG 
GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC AGCTATAACC CGCCTCTGCC ACCCCTGAAG 
ATATCTACCT CTAATGGCAG TCCAGGGTTC GACTACCATC AGCCTGGGGA CAAGTTTGAC 
GCTAGCAAGC AGAACGACCT GGGAGGCTGC AATGGGACAT CAACCATGAC TGTAATCAAA 
GATTACTATG CACTGAAGGA GAATGAAATC TGCGTGAGCC AAGGTGAGGT GGTACAGGTC 
CTCGCCGTCA ACCAGCAGAA CATGTGTCTG GTGTACCAGC CTGCCAGCGA CCATTCCCCG 
GCAGCAGAGG GCTGGGTCCC AGGCAGCATC CTGGCGCCCC TCGCCAAAGC CACAGCAGCG 
GCCGAAAGTA GTGACGGGAG CATCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317080.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172569.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X38, mRNA.

Price & Availability↑

CloneID	OMu79378
Clone ID Related Accession (Same CDS sequence)	XM_017317080.2 , XM_017317080.1
Accession Version	XM_017317080.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GACTCTGGAA 
GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC CCTACAGGCT GCCTGAATGA GGGGATGACC 
CCTCCCACAC CTCCTAGAAA CCTGGAAGAA GAACAGAAAG CCAAGGCGCT GAGAGGCAGG 
ATGTTTGTCC TGAATGAGCT GGTACAGACA GAGAAGGACT ACGTCAAGGA CCTGGGGATT 
GTGGTGGAGG GCTTCATGAA GAGAATAGAA GAGAAGGGGG TCCCTGAGGA CATGCGAGGG 
AAGGAAAAGA TCGTGTTTGG AAATATCCAC CAGATTTACG ACTGGCATAA AGATTTTTTC 
CTGGCTGAAC TGGAAAAATG TATCCAAGAG CAAGACAGAT TGGCCCAGCT CTTCATTAAA 
CACGAGCGGA AGCTGCACAT CTACGTGTGG TACTGCCAGA ACAAGCCGCG TTCCGAGTAC 
ATCGTGGCTG AGTACGATGC CTATTTTGAG GAGGTTAAAC AAGAGATCAA TCAAAGGCTG 
ACCCTTAGTG ACTTCCTGAT CAAGCCCATT CAGAGAATAA CAAAGTACCA GCTGCTACTC 
AAGGACTTCC TGCGATACAG CGAGAAGGCT GGTTTGGAGT GTTCCGATAT TGAGAAAGCA 
GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC TGTAATGACA TGATGAATCT GGGACGCCTG 
CAGGGCTTCG AGGGCACCCT GACCGCCCAG GGCAAGCTAC TGCAGCAGGA CACATTCTAT 
GTGATCGAGC TGGATGCCGG CATGCAGTCC CGGACCAAGG AGAGGCGTGT GTTTCTCTTT 
GAGCAGATTG TCATCTTCAG TGAACTGCTC CGAAAGGGGT CCCTCACCCC AGGCTACATG 
TTCAAAAGAA GCATCAAGAT GAATTACTTG GTCCTGGAAG ACAATGTGGA TGGTGACCCC 
TGCAAATTTG CACTCATGAA CAGGGAGACT TCAGAAAGGG TCATTCTACA GGCTGCCAAC 
TCAGACATCC AGCAGGCCTG GGTGCAGGAC ATCAACCAAG TCCTAGAAAC ACAGAGGGAC 
TTCTTAAATG CACTACAGTC ACCCATTGAG TATCAGCGGA AGGAGAGAAG CACAGCTGTG 
ATCCGGTCCC AGCCCCCGAG GGTTCCACAA GCCAGCCCCA GGCCCTACTC TTCTGGCCCG 
GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC AGCTATAACC CGCCTCTGCC ACCCCTGAAG 
ATATCTACCT CTAATGGCAG TCCAGGGTTC GACTACCATC AGCCTGGGGA CAAGTTTGAC 
GCTAGCAAGC AGAACGACCT GGGAGGCTGC AATGGGACAT CAACCATGAC TGTAATCAAA 
GATTACTATG CACTGAAGGA GAATGAAATC TGCGTGAGCC AAGGTGAGGT GGTACAGGTC 
CTCGCCGTCA ACCAGCAGAA CATGTGTCTG GTGTACCAGC CTGCCAGCGA CCATTCCCCG 
GCAGCAGAGG GCTGGGTCCC AGGCAGCATC CTGGCGCCCC TCGCCAAAGC CACAGCAGCG 
GCCGAAAGTA GTGACGGGAG CATCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172569.1
CDS	1578..3587
Translation	MKGGDQAYTRGPSLGWLFAKCCCCFPCRDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIK

Target ORF information:

RefSeq Version	XM_017317080.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317080.1

ORF Insert Sequence:

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GACTCTGGAA 
GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC CCTACAGGCT GCCTGAATGA GGGGATGACC 
CCTCCCACAC CTCCTAGAAA CCTGGAAGAA GAACAGAAAG CCAAGGCGCT GAGAGGCAGG 
ATGTTTGTCC TGAATGAGCT GGTACAGACA GAGAAGGACT ACGTCAAGGA CCTGGGGATT 
GTGGTGGAGG GCTTCATGAA GAGAATAGAA GAGAAGGGGG TCCCTGAGGA CATGCGAGGG 
AAGGAAAAGA TCGTGTTTGG AAATATCCAC CAGATTTACG ACTGGCATAA AGATTTTTTC 
CTGGCTGAAC TGGAAAAATG TATCCAAGAG CAAGACAGAT TGGCCCAGCT CTTCATTAAA 
CACGAGCGGA AGCTGCACAT CTACGTGTGG TACTGCCAGA ACAAGCCGCG TTCCGAGTAC 
ATCGTGGCTG AGTACGATGC CTATTTTGAG GAGGTTAAAC AAGAGATCAA TCAAAGGCTG 
ACCCTTAGTG ACTTCCTGAT CAAGCCCATT CAGAGAATAA CAAAGTACCA GCTGCTACTC 
AAGGACTTCC TGCGATACAG CGAGAAGGCT GGTTTGGAGT GTTCCGATAT TGAGAAAGCA 
GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC TGTAATGACA TGATGAATCT GGGACGCCTG 
CAGGGCTTCG AGGGCACCCT GACCGCCCAG GGCAAGCTAC TGCAGCAGGA CACATTCTAT 
GTGATCGAGC TGGATGCCGG CATGCAGTCC CGGACCAAGG AGAGGCGTGT GTTTCTCTTT 
GAGCAGATTG TCATCTTCAG TGAACTGCTC CGAAAGGGGT CCCTCACCCC AGGCTACATG 
TTCAAAAGAA GCATCAAGAT GAATTACTTG GTCCTGGAAG ACAATGTGGA TGGTGACCCC 
TGCAAATTTG CACTCATGAA CAGGGAGACT TCAGAAAGGG TCATTCTACA GGCTGCCAAC 
TCAGACATCC AGCAGGCCTG GGTGCAGGAC ATCAACCAAG TCCTAGAAAC ACAGAGGGAC 
TTCTTAAATG CACTACAGTC ACCCATTGAG TATCAGCGGA AGGAGAGAAG CACAGCTGTG 
ATCCGGTCCC AGCCCCCGAG GGTTCCACAA GCCAGCCCCA GGCCCTACTC TTCTGGCCCG 
GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC AGCTATAACC CGCCTCTGCC ACCCCTGAAG 
ATATCTACCT CTAATGGCAG TCCAGGGTTC GACTACCATC AGCCTGGGGA CAAGTTTGAC 
GCTAGCAAGC AGAACGACCT GGGAGGCTGC AATGGGACAT CAACCATGAC TGTAATCAAA 
GATTACTATG CACTGAAGGA GAATGAAATC TGCGTGAGCC AAGGTGAGGT GGTACAGGTC 
CTCGCCGTCA ACCAGCAGAA CATGTGTCTG GTGTACCAGC CTGCCAGCGA CCATTCCCCG 
GCAGCAGAGG GCTGGGTCCC AGGCAGCATC CTGGCGCCCC TCGCCAAAGC CACAGCAGCG 
GCCGAAAGTA GTGACGGGAG CATCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317081.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172570.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X42, mRNA.

Price & Availability↑

CloneID	OMu79379
Clone ID Related Accession (Same CDS sequence)	XM_017317081.2 , XM_017317081.1
Accession Version	XM_017317081.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X38
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGACTCTG 
GAAGGAGGCT CTTACCGGGG GAGCTTGAAG GACCCTACAG GCTGCCTGAA TGAGGGGATG 
ACCCCTCCCA CACCTCCTAG AAACCTGGAA GAAGAACAGA AAGCCAAGGC GCTGAGAGGC 
AGGATGTTTG TCCTGAATGA GCTGGTACAG ACAGAGAAGG ACTACGTCAA GGACCTGGGG 
ATTGTGGTGG AGGGCTTCAT GAAGAGAATA GAAGAGAAGG GGGTCCCTGA GGACATGCGA 
GGGAAGGAAA AGATCGTGTT TGGAAATATC CACCAGATTT ACGACTGGCA TAAAGATTTT 
TTCCTGGCTG AACTGGAAAA ATGTATCCAA GAGCAAGACA GATTGGCCCA GCTCTTCATT 
AAACACGAGC GGAAGCTGCA CATCTACGTG TGGTACTGCC AGAACAAGCC GCGTTCCGAG 
TACATCGTGG CTGAGTACGA TGCCTATTTT GAGGAGGTTA AACAAGAGAT CAATCAAAGG 
CTGACCCTTA GTGACTTCCT GATCAAGCCC ATTCAGAGAA TAACAAAGTA CCAGCTGCTA 
CTCAAGGACT TCCTGCGATA CAGCGAGAAG GCTGGTTTGG AGTGTTCCGA TATTGAGAAA 
GCAGTGGAGT TAATGTGCCT TGTCCCCAAG CGCTGTAATG ACATGATGAA TCTGGGACGC 
CTGCAGGGCT TCGAGGGCAC CCTGACCGCC CAGGGCAAGC TACTGCAGCA GGACACATTC 
TATGTGATCG AGCTGGATGC CGGCATGCAG TCCCGGACCA AGGAGAGGCG TGTGTTTCTC 
TTTGAGCAGA TTGTCATCTT CAGTGAACTG CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC 
ATGTTCAAAA GAAGCATCAA GATGAATTAC TTGGTCCTGG AAGACAATGT GGATGGTGAC 
CCCTGCAAAT TTGCACTCAT GAACAGGGAG ACTTCAGAAA GGGTCATTCT ACAGGCTGCC 
AACTCAGACA TCCAGCAGGC CTGGGTGCAG GACATCAACC AAGTCCTAGA AACACAGAGG 
GACTTCTTAA ATGCACTACA GTCACCCATT GAGTATCAGC GGAAGGAGAG AAGCACAGCT 
GTGATCCGGT CCCAGCCCCC GAGGGTTCCA CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC 
CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC TCCAGCTATA ACCCGCCTCT GCCACCCCTG 
AAGATATCTA CCTCTAATGG CAGTCCAGGG TTCGACTACC ATCAGCCTGG GGACAAGTTT 
GACGCTAGCA AGAACGACCT GGGAGGCTGC AATGGGACAT CAACCATGAC TGTAATCAAA 
GATTACTATG CACTGAAGGA GAATGAAATC TGCGTGAGCC AAGGTGAGGT GGTACAGGTC 
CTCGCCGTCA ACCAGCAGAA CATGTGTCTG GTGTACCAGC CTGCCAGCGA CCATTCCCCG 
GCAGCAGAGG GCTGGGTCCC AGGCAGCATC CTGGCGCCCC TCGCCAAAGC CACAGCAGCG 
GCCGAAAGTA GTGACGGGAG CATCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172570.1
CDS	1521..3530
Translation	MKGGDQAYTRGPSLGWLFAKCCCCFPCRDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIK

Target ORF information:

RefSeq Version	XM_017317081.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X42, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317081.2

ORF Insert Sequence:

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGACTCTG 
GAAGGAGGCT CTTACCGGGG GAGCTTGAAG GACCCTACAG GCTGCCTGAA TGAGGGGATG 
ACCCCTCCCA CACCTCCTAG AAACCTGGAA GAAGAACAGA AAGCCAAGGC GCTGAGAGGC 
AGGATGTTTG TCCTGAATGA GCTGGTACAG ACAGAGAAGG ACTACGTCAA GGACCTGGGG 
ATTGTGGTGG AGGGCTTCAT GAAGAGAATA GAAGAGAAGG GGGTCCCTGA GGACATGCGA 
GGGAAGGAAA AGATCGTGTT TGGAAATATC CACCAGATTT ACGACTGGCA TAAAGATTTT 
TTCCTGGCTG AACTGGAAAA ATGTATCCAA GAGCAAGACA GATTGGCCCA GCTCTTCATT 
AAACACGAGC GGAAGCTGCA CATCTACGTG TGGTACTGCC AGAACAAGCC GCGTTCCGAG 
TACATCGTGG CTGAGTACGA TGCCTATTTT GAGGAGGTTA AACAAGAGAT CAATCAAAGG 
CTGACCCTTA GTGACTTCCT GATCAAGCCC ATTCAGAGAA TAACAAAGTA CCAGCTGCTA 
CTCAAGGACT TCCTGCGATA CAGCGAGAAG GCTGGTTTGG AGTGTTCCGA TATTGAGAAA 
GCAGTGGAGT TAATGTGCCT TGTCCCCAAG CGCTGTAATG ACATGATGAA TCTGGGACGC 
CTGCAGGGCT TCGAGGGCAC CCTGACCGCC CAGGGCAAGC TACTGCAGCA GGACACATTC 
TATGTGATCG AGCTGGATGC CGGCATGCAG TCCCGGACCA AGGAGAGGCG TGTGTTTCTC 
TTTGAGCAGA TTGTCATCTT CAGTGAACTG CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC 
ATGTTCAAAA GAAGCATCAA GATGAATTAC TTGGTCCTGG AAGACAATGT GGATGGTGAC 
CCCTGCAAAT TTGCACTCAT GAACAGGGAG ACTTCAGAAA GGGTCATTCT ACAGGCTGCC 
AACTCAGACA TCCAGCAGGC CTGGGTGCAG GACATCAACC AAGTCCTAGA AACACAGAGG 
GACTTCTTAA ATGCACTACA GTCACCCATT GAGTATCAGC GGAAGGAGAG AAGCACAGCT 
GTGATCCGGT CCCAGCCCCC GAGGGTTCCA CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC 
CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC TCCAGCTATA ACCCGCCTCT GCCACCCCTG 
AAGATATCTA CCTCTAATGG CAGTCCAGGG TTCGACTACC ATCAGCCTGG GGACAAGTTT 
GACGCTAGCA AGAACGACCT GGGAGGCTGC AATGGGACAT CAACCATGAC TGTAATCAAA 
GATTACTATG CACTGAAGGA GAATGAAATC TGCGTGAGCC AAGGTGAGGT GGTACAGGTC 
CTCGCCGTCA ACCAGCAGAA CATGTGTCTG GTGTACCAGC CTGCCAGCGA CCATTCCCCG 
GCAGCAGAGG GCTGGGTCCC AGGCAGCATC CTGGCGCCCC TCGCCAAAGC CACAGCAGCG 
GCCGAAAGTA GTGACGGGAG CATCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317081.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172570.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X39, mRNA.

Price & Availability↑

CloneID	OMu79379
Clone ID Related Accession (Same CDS sequence)	XM_017317081.2 , XM_017317081.1
Accession Version	XM_017317081.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGACTCTG 
GAAGGAGGCT CTTACCGGGG GAGCTTGAAG GACCCTACAG GCTGCCTGAA TGAGGGGATG 
ACCCCTCCCA CACCTCCTAG AAACCTGGAA GAAGAACAGA AAGCCAAGGC GCTGAGAGGC 
AGGATGTTTG TCCTGAATGA GCTGGTACAG ACAGAGAAGG ACTACGTCAA GGACCTGGGG 
ATTGTGGTGG AGGGCTTCAT GAAGAGAATA GAAGAGAAGG GGGTCCCTGA GGACATGCGA 
GGGAAGGAAA AGATCGTGTT TGGAAATATC CACCAGATTT ACGACTGGCA TAAAGATTTT 
TTCCTGGCTG AACTGGAAAA ATGTATCCAA GAGCAAGACA GATTGGCCCA GCTCTTCATT 
AAACACGAGC GGAAGCTGCA CATCTACGTG TGGTACTGCC AGAACAAGCC GCGTTCCGAG 
TACATCGTGG CTGAGTACGA TGCCTATTTT GAGGAGGTTA AACAAGAGAT CAATCAAAGG 
CTGACCCTTA GTGACTTCCT GATCAAGCCC ATTCAGAGAA TAACAAAGTA CCAGCTGCTA 
CTCAAGGACT TCCTGCGATA CAGCGAGAAG GCTGGTTTGG AGTGTTCCGA TATTGAGAAA 
GCAGTGGAGT TAATGTGCCT TGTCCCCAAG CGCTGTAATG ACATGATGAA TCTGGGACGC 
CTGCAGGGCT TCGAGGGCAC CCTGACCGCC CAGGGCAAGC TACTGCAGCA GGACACATTC 
TATGTGATCG AGCTGGATGC CGGCATGCAG TCCCGGACCA AGGAGAGGCG TGTGTTTCTC 
TTTGAGCAGA TTGTCATCTT CAGTGAACTG CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC 
ATGTTCAAAA GAAGCATCAA GATGAATTAC TTGGTCCTGG AAGACAATGT GGATGGTGAC 
CCCTGCAAAT TTGCACTCAT GAACAGGGAG ACTTCAGAAA GGGTCATTCT ACAGGCTGCC 
AACTCAGACA TCCAGCAGGC CTGGGTGCAG GACATCAACC AAGTCCTAGA AACACAGAGG 
GACTTCTTAA ATGCACTACA GTCACCCATT GAGTATCAGC GGAAGGAGAG AAGCACAGCT 
GTGATCCGGT CCCAGCCCCC GAGGGTTCCA CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC 
CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC TCCAGCTATA ACCCGCCTCT GCCACCCCTG 
AAGATATCTA CCTCTAATGG CAGTCCAGGG TTCGACTACC ATCAGCCTGG GGACAAGTTT 
GACGCTAGCA AGAACGACCT GGGAGGCTGC AATGGGACAT CAACCATGAC TGTAATCAAA 
GATTACTATG CACTGAAGGA GAATGAAATC TGCGTGAGCC AAGGTGAGGT GGTACAGGTC 
CTCGCCGTCA ACCAGCAGAA CATGTGTCTG GTGTACCAGC CTGCCAGCGA CCATTCCCCG 
GCAGCAGAGG GCTGGGTCCC AGGCAGCATC CTGGCGCCCC TCGCCAAAGC CACAGCAGCG 
GCCGAAAGTA GTGACGGGAG CATCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172570.1
CDS	1578..3587
Translation	MKGGDQAYTRGPSLGWLFAKCCCCFPCRDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIK

Target ORF information:

RefSeq Version	XM_017317081.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317081.1

ORF Insert Sequence:

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGACTCTG 
GAAGGAGGCT CTTACCGGGG GAGCTTGAAG GACCCTACAG GCTGCCTGAA TGAGGGGATG 
ACCCCTCCCA CACCTCCTAG AAACCTGGAA GAAGAACAGA AAGCCAAGGC GCTGAGAGGC 
AGGATGTTTG TCCTGAATGA GCTGGTACAG ACAGAGAAGG ACTACGTCAA GGACCTGGGG 
ATTGTGGTGG AGGGCTTCAT GAAGAGAATA GAAGAGAAGG GGGTCCCTGA GGACATGCGA 
GGGAAGGAAA AGATCGTGTT TGGAAATATC CACCAGATTT ACGACTGGCA TAAAGATTTT 
TTCCTGGCTG AACTGGAAAA ATGTATCCAA GAGCAAGACA GATTGGCCCA GCTCTTCATT 
AAACACGAGC GGAAGCTGCA CATCTACGTG TGGTACTGCC AGAACAAGCC GCGTTCCGAG 
TACATCGTGG CTGAGTACGA TGCCTATTTT GAGGAGGTTA AACAAGAGAT CAATCAAAGG 
CTGACCCTTA GTGACTTCCT GATCAAGCCC ATTCAGAGAA TAACAAAGTA CCAGCTGCTA 
CTCAAGGACT TCCTGCGATA CAGCGAGAAG GCTGGTTTGG AGTGTTCCGA TATTGAGAAA 
GCAGTGGAGT TAATGTGCCT TGTCCCCAAG CGCTGTAATG ACATGATGAA TCTGGGACGC 
CTGCAGGGCT TCGAGGGCAC CCTGACCGCC CAGGGCAAGC TACTGCAGCA GGACACATTC 
TATGTGATCG AGCTGGATGC CGGCATGCAG TCCCGGACCA AGGAGAGGCG TGTGTTTCTC 
TTTGAGCAGA TTGTCATCTT CAGTGAACTG CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC 
ATGTTCAAAA GAAGCATCAA GATGAATTAC TTGGTCCTGG AAGACAATGT GGATGGTGAC 
CCCTGCAAAT TTGCACTCAT GAACAGGGAG ACTTCAGAAA GGGTCATTCT ACAGGCTGCC 
AACTCAGACA TCCAGCAGGC CTGGGTGCAG GACATCAACC AAGTCCTAGA AACACAGAGG 
GACTTCTTAA ATGCACTACA GTCACCCATT GAGTATCAGC GGAAGGAGAG AAGCACAGCT 
GTGATCCGGT CCCAGCCCCC GAGGGTTCCA CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC 
CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC TCCAGCTATA ACCCGCCTCT GCCACCCCTG 
AAGATATCTA CCTCTAATGG CAGTCCAGGG TTCGACTACC ATCAGCCTGG GGACAAGTTT 
GACGCTAGCA AGAACGACCT GGGAGGCTGC AATGGGACAT CAACCATGAC TGTAATCAAA 
GATTACTATG CACTGAAGGA GAATGAAATC TGCGTGAGCC AAGGTGAGGT GGTACAGGTC 
CTCGCCGTCA ACCAGCAGAA CATGTGTCTG GTGTACCAGC CTGCCAGCGA CCATTCCCCG 
GCAGCAGAGG GCTGGGTCCC AGGCAGCATC CTGGCGCCCC TCGCCAAAGC CACAGCAGCG 
GCCGAAAGTA GTGACGGGAG CATCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317082.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172571.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X43, mRNA.

Price & Availability↑

CloneID	OMu79380
Clone ID Related Accession (Same CDS sequence)	XM_017317082.2 , XM_017317082.1
Accession Version	XM_017317082.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X39
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GACTCTGGAA 
GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC CCTACAGGCT GCCTGAATGA GGGGATGACC 
CCTCCCACAC CTCCTAGAAA CCTGGAAGAA GAACAGAAAG CCAAGGCGCT GAGAGGCAGG 
ATGTTTGTCC TGAATGAGCT GGTACAGACA GAGAAGGACT ACGTCAAGGA CCTGGGGATT 
GTGGTGGAGG GCTTCATGAA GAGAATAGAA GAGAAGGGGG TCCCTGAGGA CATGCGAGGG 
AAGGAAAAGA TCGTGTTTGG AAATATCCAC CAGATTTACG ACTGGCATAA AGATTTTTTC 
CTGGCTGAAC TGGAAAAATG TATCCAAGAG CAAGACAGAT TGGCCCAGCT CTTCATTAAA 
CACGAGCGGA AGCTGCACAT CTACGTGTGG TACTGCCAGA ACAAGCCGCG TTCCGAGTAC 
ATCGTGGCTG AGTACGATGC CTATTTTGAG GAGGTTAAAC AAGAGATCAA TCAAAGGCTG 
ACCCTTAGTG ACTTCCTGAT CAAGCCCATT CAGAGAATAA CAAAGTACCA GCTGCTACTC 
AAGGACTTCC TGCGATACAG CGAGAAGGCT GGTTTGGAGT GTTCCGATAT TGAGAAAGCA 
GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC TGTAATGACA TGATGAATCT GGGACGCCTG 
CAGGGCTTCG AGGGCACCCT GACCGCCCAG GGCAAGCTAC TGCAGCAGGA CACATTCTAT 
GTGATCGAGC TGGATGCCGG CATGCAGTCC CGGACCAAGG AGAGGCGTGT GTTTCTCTTT 
GAGCAGATTG TCATCTTCAG TGAACTGCTC CGAAAGGGGT CCCTCACCCC AGGCTACATG 
TTCAAAAGAA GCATCAAGAT GAATTACTTG GTCCTGGAAG ACAATGTGGA TGGTGACCCC 
TGCAAATTTG CACTCATGAA CAGGGAGACT TCAGAAAGGG TCATTCTACA GGCTGCCAAC 
TCAGACATCC AGCAGGCCTG GGTGCAGGAC ATCAACCAAG TCCTAGAAAC ACAGAGGGAC 
TTCTTAAATG CACTACAGTC ACCCATTGAG TATCAGCGGA AGGAGAGAAG CACAGCTGTG 
ATCCGGTCCC AGCCCCCGAG GGTTCCACAA GCCAGCCCCA GGCCCTACTC TTCTGGCCCG 
GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC AGCTATAACC CGCCTCTGCC ACCCCTGAAG 
ATATCTACCT CTAATGGCAG TCCAGGGTTC GACTACCATC AGCCTGGGGA CAAGTTTGAC 
GCTAGCAAGA ACGACCTGGG AGGCTGCAAT GGGACATCAA CCATGACTGT AATCAAAGAT 
TACTATGCAC TGAAGGAGAA TGAAATCTGC GTGAGCCAAG GTGAGGTGGT ACAGGTCCTC 
GCCGTCAACC AGCAGAACAT GTGTCTGGTG TACCAGCCTG CCAGCGACCA TTCCCCGGCA 
GCAGAGGGCT GGGTCCCAGG CAGCATCCTG GCGCCCCTCG CCAAAGCCAC AGCAGCGGCC 
GAAAGTAGTG ACGGGAGCAT CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172571.1
CDS	1521..3527
Translation	MKGGDQAYTRGPSLGWLFAKCCCCFPCRDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIK

Target ORF information:

RefSeq Version	XM_017317082.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X43, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317082.2

ORF Insert Sequence:

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GACTCTGGAA 
GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC CCTACAGGCT GCCTGAATGA GGGGATGACC 
CCTCCCACAC CTCCTAGAAA CCTGGAAGAA GAACAGAAAG CCAAGGCGCT GAGAGGCAGG 
ATGTTTGTCC TGAATGAGCT GGTACAGACA GAGAAGGACT ACGTCAAGGA CCTGGGGATT 
GTGGTGGAGG GCTTCATGAA GAGAATAGAA GAGAAGGGGG TCCCTGAGGA CATGCGAGGG 
AAGGAAAAGA TCGTGTTTGG AAATATCCAC CAGATTTACG ACTGGCATAA AGATTTTTTC 
CTGGCTGAAC TGGAAAAATG TATCCAAGAG CAAGACAGAT TGGCCCAGCT CTTCATTAAA 
CACGAGCGGA AGCTGCACAT CTACGTGTGG TACTGCCAGA ACAAGCCGCG TTCCGAGTAC 
ATCGTGGCTG AGTACGATGC CTATTTTGAG GAGGTTAAAC AAGAGATCAA TCAAAGGCTG 
ACCCTTAGTG ACTTCCTGAT CAAGCCCATT CAGAGAATAA CAAAGTACCA GCTGCTACTC 
AAGGACTTCC TGCGATACAG CGAGAAGGCT GGTTTGGAGT GTTCCGATAT TGAGAAAGCA 
GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC TGTAATGACA TGATGAATCT GGGACGCCTG 
CAGGGCTTCG AGGGCACCCT GACCGCCCAG GGCAAGCTAC TGCAGCAGGA CACATTCTAT 
GTGATCGAGC TGGATGCCGG CATGCAGTCC CGGACCAAGG AGAGGCGTGT GTTTCTCTTT 
GAGCAGATTG TCATCTTCAG TGAACTGCTC CGAAAGGGGT CCCTCACCCC AGGCTACATG 
TTCAAAAGAA GCATCAAGAT GAATTACTTG GTCCTGGAAG ACAATGTGGA TGGTGACCCC 
TGCAAATTTG CACTCATGAA CAGGGAGACT TCAGAAAGGG TCATTCTACA GGCTGCCAAC 
TCAGACATCC AGCAGGCCTG GGTGCAGGAC ATCAACCAAG TCCTAGAAAC ACAGAGGGAC 
TTCTTAAATG CACTACAGTC ACCCATTGAG TATCAGCGGA AGGAGAGAAG CACAGCTGTG 
ATCCGGTCCC AGCCCCCGAG GGTTCCACAA GCCAGCCCCA GGCCCTACTC TTCTGGCCCG 
GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC AGCTATAACC CGCCTCTGCC ACCCCTGAAG 
ATATCTACCT CTAATGGCAG TCCAGGGTTC GACTACCATC AGCCTGGGGA CAAGTTTGAC 
GCTAGCAAGA ACGACCTGGG AGGCTGCAAT GGGACATCAA CCATGACTGT AATCAAAGAT 
TACTATGCAC TGAAGGAGAA TGAAATCTGC GTGAGCCAAG GTGAGGTGGT ACAGGTCCTC 
GCCGTCAACC AGCAGAACAT GTGTCTGGTG TACCAGCCTG CCAGCGACCA TTCCCCGGCA 
GCAGAGGGCT GGGTCCCAGG CAGCATCCTG GCGCCCCTCG CCAAAGCCAC AGCAGCGGCC 
GAAAGTAGTG ACGGGAGCAT CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317082.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172571.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X40, mRNA.

Price & Availability↑

CloneID	OMu79380
Clone ID Related Accession (Same CDS sequence)	XM_017317082.2 , XM_017317082.1
Accession Version	XM_017317082.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X37
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GACTCTGGAA 
GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC CCTACAGGCT GCCTGAATGA GGGGATGACC 
CCTCCCACAC CTCCTAGAAA CCTGGAAGAA GAACAGAAAG CCAAGGCGCT GAGAGGCAGG 
ATGTTTGTCC TGAATGAGCT GGTACAGACA GAGAAGGACT ACGTCAAGGA CCTGGGGATT 
GTGGTGGAGG GCTTCATGAA GAGAATAGAA GAGAAGGGGG TCCCTGAGGA CATGCGAGGG 
AAGGAAAAGA TCGTGTTTGG AAATATCCAC CAGATTTACG ACTGGCATAA AGATTTTTTC 
CTGGCTGAAC TGGAAAAATG TATCCAAGAG CAAGACAGAT TGGCCCAGCT CTTCATTAAA 
CACGAGCGGA AGCTGCACAT CTACGTGTGG TACTGCCAGA ACAAGCCGCG TTCCGAGTAC 
ATCGTGGCTG AGTACGATGC CTATTTTGAG GAGGTTAAAC AAGAGATCAA TCAAAGGCTG 
ACCCTTAGTG ACTTCCTGAT CAAGCCCATT CAGAGAATAA CAAAGTACCA GCTGCTACTC 
AAGGACTTCC TGCGATACAG CGAGAAGGCT GGTTTGGAGT GTTCCGATAT TGAGAAAGCA 
GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC TGTAATGACA TGATGAATCT GGGACGCCTG 
CAGGGCTTCG AGGGCACCCT GACCGCCCAG GGCAAGCTAC TGCAGCAGGA CACATTCTAT 
GTGATCGAGC TGGATGCCGG CATGCAGTCC CGGACCAAGG AGAGGCGTGT GTTTCTCTTT 
GAGCAGATTG TCATCTTCAG TGAACTGCTC CGAAAGGGGT CCCTCACCCC AGGCTACATG 
TTCAAAAGAA GCATCAAGAT GAATTACTTG GTCCTGGAAG ACAATGTGGA TGGTGACCCC 
TGCAAATTTG CACTCATGAA CAGGGAGACT TCAGAAAGGG TCATTCTACA GGCTGCCAAC 
TCAGACATCC AGCAGGCCTG GGTGCAGGAC ATCAACCAAG TCCTAGAAAC ACAGAGGGAC 
TTCTTAAATG CACTACAGTC ACCCATTGAG TATCAGCGGA AGGAGAGAAG CACAGCTGTG 
ATCCGGTCCC AGCCCCCGAG GGTTCCACAA GCCAGCCCCA GGCCCTACTC TTCTGGCCCG 
GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC AGCTATAACC CGCCTCTGCC ACCCCTGAAG 
ATATCTACCT CTAATGGCAG TCCAGGGTTC GACTACCATC AGCCTGGGGA CAAGTTTGAC 
GCTAGCAAGA ACGACCTGGG AGGCTGCAAT GGGACATCAA CCATGACTGT AATCAAAGAT 
TACTATGCAC TGAAGGAGAA TGAAATCTGC GTGAGCCAAG GTGAGGTGGT ACAGGTCCTC 
GCCGTCAACC AGCAGAACAT GTGTCTGGTG TACCAGCCTG CCAGCGACCA TTCCCCGGCA 
GCAGAGGGCT GGGTCCCAGG CAGCATCCTG GCGCCCCTCG CCAAAGCCAC AGCAGCGGCC 
GAAAGTAGTG ACGGGAGCAT CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172571.1
CDS	1578..3584
Translation	MKGGDQAYTRGPSLGWLFAKCCCCFPCRDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIK

Target ORF information:

RefSeq Version	XM_017317082.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317082.1

ORF Insert Sequence:

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GACTCTGGAA 
GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC CCTACAGGCT GCCTGAATGA GGGGATGACC 
CCTCCCACAC CTCCTAGAAA CCTGGAAGAA GAACAGAAAG CCAAGGCGCT GAGAGGCAGG 
ATGTTTGTCC TGAATGAGCT GGTACAGACA GAGAAGGACT ACGTCAAGGA CCTGGGGATT 
GTGGTGGAGG GCTTCATGAA GAGAATAGAA GAGAAGGGGG TCCCTGAGGA CATGCGAGGG 
AAGGAAAAGA TCGTGTTTGG AAATATCCAC CAGATTTACG ACTGGCATAA AGATTTTTTC 
CTGGCTGAAC TGGAAAAATG TATCCAAGAG CAAGACAGAT TGGCCCAGCT CTTCATTAAA 
CACGAGCGGA AGCTGCACAT CTACGTGTGG TACTGCCAGA ACAAGCCGCG TTCCGAGTAC 
ATCGTGGCTG AGTACGATGC CTATTTTGAG GAGGTTAAAC AAGAGATCAA TCAAAGGCTG 
ACCCTTAGTG ACTTCCTGAT CAAGCCCATT CAGAGAATAA CAAAGTACCA GCTGCTACTC 
AAGGACTTCC TGCGATACAG CGAGAAGGCT GGTTTGGAGT GTTCCGATAT TGAGAAAGCA 
GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC TGTAATGACA TGATGAATCT GGGACGCCTG 
CAGGGCTTCG AGGGCACCCT GACCGCCCAG GGCAAGCTAC TGCAGCAGGA CACATTCTAT 
GTGATCGAGC TGGATGCCGG CATGCAGTCC CGGACCAAGG AGAGGCGTGT GTTTCTCTTT 
GAGCAGATTG TCATCTTCAG TGAACTGCTC CGAAAGGGGT CCCTCACCCC AGGCTACATG 
TTCAAAAGAA GCATCAAGAT GAATTACTTG GTCCTGGAAG ACAATGTGGA TGGTGACCCC 
TGCAAATTTG CACTCATGAA CAGGGAGACT TCAGAAAGGG TCATTCTACA GGCTGCCAAC 
TCAGACATCC AGCAGGCCTG GGTGCAGGAC ATCAACCAAG TCCTAGAAAC ACAGAGGGAC 
TTCTTAAATG CACTACAGTC ACCCATTGAG TATCAGCGGA AGGAGAGAAG CACAGCTGTG 
ATCCGGTCCC AGCCCCCGAG GGTTCCACAA GCCAGCCCCA GGCCCTACTC TTCTGGCCCG 
GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC AGCTATAACC CGCCTCTGCC ACCCCTGAAG 
ATATCTACCT CTAATGGCAG TCCAGGGTTC GACTACCATC AGCCTGGGGA CAAGTTTGAC 
GCTAGCAAGA ACGACCTGGG AGGCTGCAAT GGGACATCAA CCATGACTGT AATCAAAGAT 
TACTATGCAC TGAAGGAGAA TGAAATCTGC GTGAGCCAAG GTGAGGTGGT ACAGGTCCTC 
GCCGTCAACC AGCAGAACAT GTGTCTGGTG TACCAGCCTG CCAGCGACCA TTCCCCGGCA 
GCAGAGGGCT GGGTCCCAGG CAGCATCCTG GCGCCCCTCG CCAAAGCCAC AGCAGCGGCC 
GAAAGTAGTG ACGGGAGCAT CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522385.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522448.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X37, mRNA.

Price & Availability↑

CloneID	OMu52301
Clone ID Related Accession (Same CDS sequence)	XM_006522385.4 , XM_006522385.3
Accession Version	XM_006522385.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522385.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGTCGTCT 
TTGCTAGCAG CCCGGCAGGC TCCCCCTGAC GTGCCTACTG CTGCAGACCT TGTCAGTGCA 
ATAGAAAAGT TGGTCAAAAA TAAGCTGACT CTGGAAGGAG GCTCTTACCG GGGGAGCTTG 
AAGGACCCTA CAGGCTGCCT GAATGAGGGG ATGACCCCTC CCACACCTCC TAGAAACCTG 
GAAGAAGAAC AGAAAGCCAA GGCGCTGAGA GGCAGGATGT TTGTCCTGAA TGAGCTGGTA 
CAGACAGAGA AGGACTACGT CAAGGACCTG GGGATTGTGG TGGAGGGCTT CATGAAGAGA 
ATAGAAGAGA AGGGGGTCCC TGAGGACATG CGAGGGAAGG AAAAGATCGT GTTTGGAAAT 
ATCCACCAGA TTTACGACTG GCATAAAGAT TTTTTCCTGG CTGAACTGGA AAAATGTATC 
CAAGAGCAAG ACAGATTGGC CCAGCTCTTC ATTAAACACG AGCGGAAGCT GCACATCTAC 
GTGTGGTACT GCCAGAACAA GCCGCGTTCC GAGTACATCG TGGCTGAGTA CGATGCCTAT 
TTTGAGGAGG TTAAACAAGA GATCAATCAA AGGCTGACCC TTAGTGACTT CCTGATCAAG 
CCCATTCAGA GAATAACAAA GTACCAGCTG CTACTCAAGG ACTTCCTGCG ATACAGCGAG 
AAGGCTGGTT TGGAGTGTTC CGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTCCCC 
AAGCGCTGTA ATGACATGAT GAATCTGGGA CGCCTGCAGG GCTTCGAGGG CACCCTGACC 
GCCCAGGGCA AGCTACTGCA GCAGGACACA TTCTATGTGA TCGAGCTGGA TGCCGGCATG 
CAGTCCCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AGATTGTCAT CTTCAGTGAA 
CTGCTCCGAA AGGGGTCCCT CACCCCAGGC TACATGTTCA AAAGAAGCAT CAAGATGAAT 
TACTTGGTCC TGGAAGACAA TGTGGATGGT GACCCCTGCA AATTTGCACT CATGAACAGG 
GAGACTTCAG AAAGGGTCAT TCTACAGGCT GCCAACTCAG ACATCCAGCA GGCCTGGGTG 
CAGGACATCA ACCAAGTCCT AGAAACACAG AGGGACTTCT TAAATGCACT ACAGTCACCC 
ATTGAGTATC AGCGGAAGGA GAGAAGCACA GCTGTGATCC GGTCCCAGCC CCCGAGGGTT 
CCACAAGCCA GCCCCAGGCC CTACTCTTCT GGCCCGGTGG GCTCAGAGAA GCCCCCCAAG 
GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCTAA TGGCAGTCCA 
GGGTTCGACT ACCATCAGCC TGGGGACAAG TTTGACGCTA GCAAGCAGAA CGACCTGGGA 
GGCTGCAATG GGACATCAAC CATGACTGTA ATCAAAGATT ACTATGCACT GAAGGAGAAT 
GAAATCTGCG TGAGCCAAGG TGAGGTGGTA CAGGTCCTCG CCGTCAACCA GCAGAACATG 
TGTCTGGTGT ACCAGCCTGC CAGCGACCAT TCCCCGGCAG CAGAGGGCTG GGTCCCAGGC 
AGCATCCTGG CGCCCCTCGC CAAAGCCACA GCAGCGGCCG AAAGTAGTGA CGGGAGCATC 
AAGAAGTCAT GCTCTTGGCA CACTCTACGC ATGAGAAAGC GGGCAGACGT GGAGAACTCG 
GGTAAAAATG AAGCCACAGG GCCTCGTAAA CCCAAGGATA TTCTGGGCAA CAAAGTCTCT 
GTTAAAGAGA CGAACAGTTC CGAGGAGTCA GAGTGTGATG ATCTTGACCC TAATACTAGT 
ATGGAGATCT TGAATCCAAA TTTCATCCAG GAAGTGGCTC CAGAATTCCT CGTGCCCTTA 
GTAGATGTGA CCTGTTTGCT TGGAGACACA GTGCTACTGC AGTGTAAAGC CTGTGGGCGG 
CCAAAGCCCA GCATCACCTG GAAGGGTCCG GATCAGAACA TCCTAGACAC TGACAACAGT 
TCAGCAACAT ACACCATCTC CTCCTGTGAT TCTGGGGAAA GCACCCTCAA GATCTGCAAC 
CTGATGCCCC AGGATAGTGG GATTTATACC TGCATAGCAG CAAACGACCA CGGGACTGCA 
TCCACATCTG CTACAGTTAA GGTGCAAGGT GTCCCCGCAG CCCCCAACCG CCCAATCGCA 
CAGGAGAGGA GCTGCACCTC CGTCATCCTC CGCTGGCTGC CCCCAGCCAG CACCGGAAAC 
TGCACTATTT CGGGGTACAC AGTGGAGTAT CGCGAGGAAG GTTCCCAGGT CTGGCAGCAA 
TCCGTAGCTT CGACCTTGGA CACTTACCTG GTCATTGAAG ACCTTAGTCC TGGGTGCCCT 
TATCAGTTCA GGGTCAGTGC CAGCAACCCC TGGGGAATCA GCCTTCCCAG TGAGCCCTCG 
GAGTTTGTGC GCCTTCCAGA GTACGATGCA GCCGCCGACG GTGCCACCAT TTCTTGGAAG 
GAAAATTTTG ATTCTGCTTA CACTGAACTG AACGAGATTG GCAGAGGCCG TTTCTCTATT 
GTAAAGAAAT GCATTCACAA AGCTACCCGC AAAGATGTTG CTGTGAAATT TGTGAGCAAA 
AAAATGAAGA AGAAGGAGCA GGCTGCTCAC GAGGCCGCCC TGCTCCAGCA CCTGCAGCAC 
CCTCAGTATG TCACGCTCCA TGACACCTAC GAGTCCCCCA CATCCTACAT CCTGATTTTG 
GAACTGATGG ATGACGGCCG ACTCTTGGAC TACCTTATGA ATCATGATGA GCTGATGGAA 
GAAAAGGTAG CTTTCTACAT TCGAGACATC ATGGAAGCTC TGCAGTACCT GCACAACTGT 
AGGGTCGCAC ATTTGGACAT AAAGCCCGAA AACCTGCTCA TTGACCTCCG GATCCCAGTG 
CCTCGAGTGA AGCTCATCGA CTTGGAAGAC GCTGTCCAGA TCTCAGGCCA CTTCCATATC 
CACCATCTGC TGGGGAACCC TGAGTTCGCT GCCCCCGAAG TGATCCAAGG CATCCCTGTC 
TCCCTGGGCA CAGACATCTG GAGCATCGGG GTTCTGACAT ATGTCATGCT TAGTGGGGTC 
TCCCCCTTCT TAGATGAGAG CAAAGAGGAA ACCTGTATCA ACGTGTGCAG GGTGGACTTT 
AGCTTCCCCC ACGAGTACTT CTGCGGTGTG AGCAATGCTG CCAGGGATTT CATCAACGTA 
ATCTTACAGG AAGACTTCCG GAGGCGGCCC ACAGCAGCCA CGTGCCTGCA GCATCCCTGG 
CTACAGCCCC ACAACGGCAG CTACTCCAAG ATCCCCTTGG ACACCTCCCG TCTGGCCTGC 
TTCATAGAAC GCCGCAAGCA CCAGAATGAT GTGCGGCCCA TTCCCAATGT CAAGAGTTAC 
ATCGTCAATA GGGTGAACCA AGGGACCTCG CTATCCCACA ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522448.1
CDS	1521..5408
Translation	MKGGDQAYTRGPSLGWLFAKCCCCFPCRDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_006522385.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522385.4

ORF Insert Sequence:

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGTCGTCT 
TTGCTAGCAG CCCGGCAGGC TCCCCCTGAC GTGCCTACTG CTGCAGACCT TGTCAGTGCA 
ATAGAAAAGT TGGTCAAAAA TAAGCTGACT CTGGAAGGAG GCTCTTACCG GGGGAGCTTG 
AAGGACCCTA CAGGCTGCCT GAATGAGGGG ATGACCCCTC CCACACCTCC TAGAAACCTG 
GAAGAAGAAC AGAAAGCCAA GGCGCTGAGA GGCAGGATGT TTGTCCTGAA TGAGCTGGTA 
CAGACAGAGA AGGACTACGT CAAGGACCTG GGGATTGTGG TGGAGGGCTT CATGAAGAGA 
ATAGAAGAGA AGGGGGTCCC TGAGGACATG CGAGGGAAGG AAAAGATCGT GTTTGGAAAT 
ATCCACCAGA TTTACGACTG GCATAAAGAT TTTTTCCTGG CTGAACTGGA AAAATGTATC 
CAAGAGCAAG ACAGATTGGC CCAGCTCTTC ATTAAACACG AGCGGAAGCT GCACATCTAC 
GTGTGGTACT GCCAGAACAA GCCGCGTTCC GAGTACATCG TGGCTGAGTA CGATGCCTAT 
TTTGAGGAGG TTAAACAAGA GATCAATCAA AGGCTGACCC TTAGTGACTT CCTGATCAAG 
CCCATTCAGA GAATAACAAA GTACCAGCTG CTACTCAAGG ACTTCCTGCG ATACAGCGAG 
AAGGCTGGTT TGGAGTGTTC CGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTCCCC 
AAGCGCTGTA ATGACATGAT GAATCTGGGA CGCCTGCAGG GCTTCGAGGG CACCCTGACC 
GCCCAGGGCA AGCTACTGCA GCAGGACACA TTCTATGTGA TCGAGCTGGA TGCCGGCATG 
CAGTCCCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AGATTGTCAT CTTCAGTGAA 
CTGCTCCGAA AGGGGTCCCT CACCCCAGGC TACATGTTCA AAAGAAGCAT CAAGATGAAT 
TACTTGGTCC TGGAAGACAA TGTGGATGGT GACCCCTGCA AATTTGCACT CATGAACAGG 
GAGACTTCAG AAAGGGTCAT TCTACAGGCT GCCAACTCAG ACATCCAGCA GGCCTGGGTG 
CAGGACATCA ACCAAGTCCT AGAAACACAG AGGGACTTCT TAAATGCACT ACAGTCACCC 
ATTGAGTATC AGCGGAAGGA GAGAAGCACA GCTGTGATCC GGTCCCAGCC CCCGAGGGTT 
CCACAAGCCA GCCCCAGGCC CTACTCTTCT GGCCCGGTGG GCTCAGAGAA GCCCCCCAAG 
GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCTAA TGGCAGTCCA 
GGGTTCGACT ACCATCAGCC TGGGGACAAG TTTGACGCTA GCAAGCAGAA CGACCTGGGA 
GGCTGCAATG GGACATCAAC CATGACTGTA ATCAAAGATT ACTATGCACT GAAGGAGAAT 
GAAATCTGCG TGAGCCAAGG TGAGGTGGTA CAGGTCCTCG CCGTCAACCA GCAGAACATG 
TGTCTGGTGT ACCAGCCTGC CAGCGACCAT TCCCCGGCAG CAGAGGGCTG GGTCCCAGGC 
AGCATCCTGG CGCCCCTCGC CAAAGCCACA GCAGCGGCCG AAAGTAGTGA CGGGAGCATC 
AAGAAGTCAT GCTCTTGGCA CACTCTACGC ATGAGAAAGC GGGCAGACGT GGAGAACTCG 
GGTAAAAATG AAGCCACAGG GCCTCGTAAA CCCAAGGATA TTCTGGGCAA CAAAGTCTCT 
GTTAAAGAGA CGAACAGTTC CGAGGAGTCA GAGTGTGATG ATCTTGACCC TAATACTAGT 
ATGGAGATCT TGAATCCAAA TTTCATCCAG GAAGTGGCTC CAGAATTCCT CGTGCCCTTA 
GTAGATGTGA CCTGTTTGCT TGGAGACACA GTGCTACTGC AGTGTAAAGC CTGTGGGCGG 
CCAAAGCCCA GCATCACCTG GAAGGGTCCG GATCAGAACA TCCTAGACAC TGACAACAGT 
TCAGCAACAT ACACCATCTC CTCCTGTGAT TCTGGGGAAA GCACCCTCAA GATCTGCAAC 
CTGATGCCCC AGGATAGTGG GATTTATACC TGCATAGCAG CAAACGACCA CGGGACTGCA 
TCCACATCTG CTACAGTTAA GGTGCAAGGT GTCCCCGCAG CCCCCAACCG CCCAATCGCA 
CAGGAGAGGA GCTGCACCTC CGTCATCCTC CGCTGGCTGC CCCCAGCCAG CACCGGAAAC 
TGCACTATTT CGGGGTACAC AGTGGAGTAT CGCGAGGAAG GTTCCCAGGT CTGGCAGCAA 
TCCGTAGCTT CGACCTTGGA CACTTACCTG GTCATTGAAG ACCTTAGTCC TGGGTGCCCT 
TATCAGTTCA GGGTCAGTGC CAGCAACCCC TGGGGAATCA GCCTTCCCAG TGAGCCCTCG 
GAGTTTGTGC GCCTTCCAGA GTACGATGCA GCCGCCGACG GTGCCACCAT TTCTTGGAAG 
GAAAATTTTG ATTCTGCTTA CACTGAACTG AACGAGATTG GCAGAGGCCG TTTCTCTATT 
GTAAAGAAAT GCATTCACAA AGCTACCCGC AAAGATGTTG CTGTGAAATT TGTGAGCAAA 
AAAATGAAGA AGAAGGAGCA GGCTGCTCAC GAGGCCGCCC TGCTCCAGCA CCTGCAGCAC 
CCTCAGTATG TCACGCTCCA TGACACCTAC GAGTCCCCCA CATCCTACAT CCTGATTTTG 
GAACTGATGG ATGACGGCCG ACTCTTGGAC TACCTTATGA ATCATGATGA GCTGATGGAA 
GAAAAGGTAG CTTTCTACAT TCGAGACATC ATGGAAGCTC TGCAGTACCT GCACAACTGT 
AGGGTCGCAC ATTTGGACAT AAAGCCCGAA AACCTGCTCA TTGACCTCCG GATCCCAGTG 
CCTCGAGTGA AGCTCATCGA CTTGGAAGAC GCTGTCCAGA TCTCAGGCCA CTTCCATATC 
CACCATCTGC TGGGGAACCC TGAGTTCGCT GCCCCCGAAG TGATCCAAGG CATCCCTGTC 
TCCCTGGGCA CAGACATCTG GAGCATCGGG GTTCTGACAT ATGTCATGCT TAGTGGGGTC 
TCCCCCTTCT TAGATGAGAG CAAAGAGGAA ACCTGTATCA ACGTGTGCAG GGTGGACTTT 
AGCTTCCCCC ACGAGTACTT CTGCGGTGTG AGCAATGCTG CCAGGGATTT CATCAACGTA 
ATCTTACAGG AAGACTTCCG GAGGCGGCCC ACAGCAGCCA CGTGCCTGCA GCATCCCTGG 
CTACAGCCCC ACAACGGCAG CTACTCCAAG ATCCCCTTGG ACACCTCCCG TCTGGCCTGC 
TTCATAGAAC GCCGCAAGCA CCAGAATGAT GTGCGGCCCA TTCCCAATGT CAAGAGTTAC 
ATCGTCAATA GGGTGAACCA AGGGACCTCG CTATCCCACA ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317077.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172566.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X35, mRNA.

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CloneID	OMu79375
Clone ID Related Accession (Same CDS sequence)	XM_017317077.1 , XM_017317077.2
Accession Version	XM_017317077.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGTCGTCT 
TTGCTAGCAG CCCGGCAGGC TCCCCCTGAC GTGCCTACTG CTGCAGACCT TGTCAGTGCA 
ATAGAAAAGT TGGTCAAAAA TAAGCTGACT CTGGAAGGAG GCTCTTACCG GGGGAGCTTG 
AAGGACCCTA CAGGCTGCCT GAATGAGGGG ATGACCCCTC CCACACCTCC TAGAAACCTG 
GAAGAAGAAC AGAAAGCCAA GGCGCTGAGA GGCAGGATGT TTGTCCTGAA TGAGCTGGTA 
CAGACAGAGA AGGACTACGT CAAGGACCTG GGGATTGTGG TGGAGGGCTT CATGAAGAGA 
ATAGAAGAGA AGGGGGTCCC TGAGGACATG CGAGGGAAGG AAAAGATCGT GTTTGGAAAT 
ATCCACCAGA TTTACGACTG GCATAAAGAT TTTTTCCTGG CTGAACTGGA AAAATGTATC 
CAAGAGCAAG ACAGATTGGC CCAGCTCTTC ATTAAACACG AGCGGAAGCT GCACATCTAC 
GTGTGGTACT GCCAGAACAA GCCGCGTTCC GAGTACATCG TGGCTGAGTA CGATGCCTAT 
TTTGAGGAGG TTAAACAAGA GATCAATCAA AGGCTGACCC TTAGTGACTT CCTGATCAAG 
CCCATTCAGA GAATAACAAA GTACCAGCTG CTACTCAAGG ACTTCCTGCG ATACAGCGAG 
AAGGCTGGTT TGGAGTGTTC CGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTCCCC 
AAGCGCTGTA ATGACATGAT GAATCTGGGA CGCCTGCAGG GCTTCGAGGG CACCCTGACC 
GCCCAGGGCA AGCTACTGCA GCAGGACACA TTCTATGTGA TCGAGCTGGA TGCCGGCATG 
CAGTCCCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AGATTGTCAT CTTCAGTGAA 
CTGCTCCGAA AGGGGTCCCT CACCCCAGGC TACATGTTCA AAAGAAGCAT CAAGATGAAT 
TACTTGGTCC TGGAAGACAA TGTGGATGGT GACCCCTGCA AATTTGCACT CATGAACAGG 
GAGACTTCAG AAAGGGTCAT TCTACAGGCT GCCAACTCAG ACATCCAGCA GGCCTGGGTG 
CAGGACATCA ACCAAGTCCT AGAAACACAG AGGGACTTCT TAAATGCACT ACAGTCACCC 
ATTGAGTATC AGCGGAAGGA GAGAAGCACA GCTGTGATCC GGTCCCAGCC CCCGAGGGTT 
CCACAAGCCA GCCCCAGGCC CTACTCTTCT GGCCCGGTGG GCTCAGAGAA GCCCCCCAAG 
GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCTAA TGGCAGTCCA 
GGGTTCGACT ACCATCAGCC TGGGGACAAG TTTGACGCTA GCAAGAACGA CCTGGGAGGC 
TGCAATGGGA CATCAACCAT GACTGTAATC AAAGATTACT ATGCACTGAA GGAGAATGAA 
ATCTGCGTGA GCCAAGGTGA GGTGGTACAG GTCCTCGCCG TCAACCAGCA GAACATGTGT 
CTGGTGTACC AGCCTGCCAG CGACCATTCC CCGGCAGCAG AGGGCTGGGT CCCAGGCAGC 
ATCCTGGCGC CCCTCGCCAA AGCCACAGCA GCGGCCGAAA GTAGTGACGG GAGCATCAAG 
AAGTCATGCT CTTGGCACAC TCTACGCATG AGAAAGCGGG CAGACGTGGA GAACTCGGGT 
AAAAATGAAG CCACAGGGCC TCGTAAACCC AAGGATATTC TGGGCAACAA AGTCTCTGTT 
AAAGAGACGA ACAGTTCCGA GGAGTCAGAG TGTGATGATC TTGACCCTAA TACTAGTATG 
GAGATCTTGA ATCCAAATTT CATCCAGGAA GTGGCTCCAG AATTCCTCGT GCCCTTAGTA 
GATGTGACCT GTTTGCTTGG AGACACAGTG CTACTGCAGT GTAAAGCCTG TGGGCGGCCA 
AAGCCCAGCA TCACCTGGAA GGGTCCGGAT CAGAACATCC TAGACACTGA CAACAGTTCA 
GCAACATACA CCATCTCCTC CTGTGATTCT GGGGAAAGCA CCCTCAAGAT CTGCAACCTG 
ATGCCCCAGG ATAGTGGGAT TTATACCTGC ATAGCAGCAA ACGACCACGG GACTGCATCC 
ACATCTGCTA CAGTTAAGGT GCAAGGTGTC CCCGCAGCCC CCAACCGCCC AATCGCACAG 
GAGAGGAGCT GCACCTCCGT CATCCTCCGC TGGCTGCCCC CAGCCAGCAC CGGAAACTGC 
ACTATTTCGG GGTACACAGT GGAGTATCGC GAGGAAGGTT CCCAGGTCTG GCAGCAATCC 
GTAGCTTCGA CCTTGGACAC TTACCTGGTC ATTGAAGACC TTAGTCCTGG GTGCCCTTAT 
CAGTTCAGGG TCAGTGCCAG CAACCCCTGG GGAATCAGCC TTCCCAGTGA GCCCTCGGAG 
TTTGTGCGCC TTCCAGAGTA CGATGCAGCC GCCGACGGTG CCACCATTTC TTGGAAGGAA 
AATTTTGATT CTGCTTACAC TGAACTGAAC GAGATTGGCA GAGGCCGTTT CTCTATTGTA 
AAGAAATGCA TTCACAAAGC TACCCGCAAA GATGTTGCTG TGAAATTTGT GAGCAAAAAA 
ATGAAGAAGA AGGAGCAGGC TGCTCACGAG GCCGCCCTGC TCCAGCACCT GCAGCACCCT 
CAGTATGTCA CGCTCCATGA CACCTACGAG TCCCCCACAT CCTACATCCT GATTTTGGAA 
CTGATGGATG ACGGCCGACT CTTGGACTAC CTTATGAATC ATGATGAGCT GATGGAAGAA 
AAGGTAGCTT TCTACATTCG AGACATCATG GAAGCTCTGC AGTACCTGCA CAACTGTAGG 
GTCGCACATT TGGACATAAA GCCCGAAAAC CTGCTCATTG ACCTCCGGAT CCCAGTGCCT 
CGAGTGAAGC TCATCGACTT GGAAGACGCT GTCCAGATCT CAGGCCACTT CCATATCCAC 
CATCTGCTGG GGAACCCTGA GTTCGCTGCC CCCGAAGTGA TCCAAGGCAT CCCTGTCTCC 
CTGGGCACAG ACATCTGGAG CATCGGGGTT CTGACATATG TCATGCTTAG TGGGGTCTCC 
CCCTTCTTAG ATGAGAGCAA AGAGGAAACC TGTATCAACG TGTGCAGGGT GGACTTTAGC 
TTCCCCCACG AGTACTTCTG CGGTGTGAGC AATGCTGCCA GGGATTTCAT CAACGTAATC 
TTACAGGAAG ACTTCCGGAG GCGGCCCACA GCAGCCACGT GCCTGCAGCA TCCCTGGCTA 
CAGCCCCACA ACGGCAGCTA CTCCAAGATC CCCTTGGACA CCTCCCGTCT GGCCTGCTTC 
ATAGAACGCC GCAAGCACCA GAATGATGTG CGGCCCATTC CCAATGTCAA GAGTTACATC 
GTCAATAGGG TGAACCAAGG GACCTCGCTA TCCCACAACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172566.1
CDS	1578..5462
Translation	MKGGDQAYTRGPSLGWLFAKCCCCFPCRDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_017317077.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317077.1

ORF Insert Sequence:

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGTCGTCT 
TTGCTAGCAG CCCGGCAGGC TCCCCCTGAC GTGCCTACTG CTGCAGACCT TGTCAGTGCA 
ATAGAAAAGT TGGTCAAAAA TAAGCTGACT CTGGAAGGAG GCTCTTACCG GGGGAGCTTG 
AAGGACCCTA CAGGCTGCCT GAATGAGGGG ATGACCCCTC CCACACCTCC TAGAAACCTG 
GAAGAAGAAC AGAAAGCCAA GGCGCTGAGA GGCAGGATGT TTGTCCTGAA TGAGCTGGTA 
CAGACAGAGA AGGACTACGT CAAGGACCTG GGGATTGTGG TGGAGGGCTT CATGAAGAGA 
ATAGAAGAGA AGGGGGTCCC TGAGGACATG CGAGGGAAGG AAAAGATCGT GTTTGGAAAT 
ATCCACCAGA TTTACGACTG GCATAAAGAT TTTTTCCTGG CTGAACTGGA AAAATGTATC 
CAAGAGCAAG ACAGATTGGC CCAGCTCTTC ATTAAACACG AGCGGAAGCT GCACATCTAC 
GTGTGGTACT GCCAGAACAA GCCGCGTTCC GAGTACATCG TGGCTGAGTA CGATGCCTAT 
TTTGAGGAGG TTAAACAAGA GATCAATCAA AGGCTGACCC TTAGTGACTT CCTGATCAAG 
CCCATTCAGA GAATAACAAA GTACCAGCTG CTACTCAAGG ACTTCCTGCG ATACAGCGAG 
AAGGCTGGTT TGGAGTGTTC CGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTCCCC 
AAGCGCTGTA ATGACATGAT GAATCTGGGA CGCCTGCAGG GCTTCGAGGG CACCCTGACC 
GCCCAGGGCA AGCTACTGCA GCAGGACACA TTCTATGTGA TCGAGCTGGA TGCCGGCATG 
CAGTCCCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AGATTGTCAT CTTCAGTGAA 
CTGCTCCGAA AGGGGTCCCT CACCCCAGGC TACATGTTCA AAAGAAGCAT CAAGATGAAT 
TACTTGGTCC TGGAAGACAA TGTGGATGGT GACCCCTGCA AATTTGCACT CATGAACAGG 
GAGACTTCAG AAAGGGTCAT TCTACAGGCT GCCAACTCAG ACATCCAGCA GGCCTGGGTG 
CAGGACATCA ACCAAGTCCT AGAAACACAG AGGGACTTCT TAAATGCACT ACAGTCACCC 
ATTGAGTATC AGCGGAAGGA GAGAAGCACA GCTGTGATCC GGTCCCAGCC CCCGAGGGTT 
CCACAAGCCA GCCCCAGGCC CTACTCTTCT GGCCCGGTGG GCTCAGAGAA GCCCCCCAAG 
GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCTAA TGGCAGTCCA 
GGGTTCGACT ACCATCAGCC TGGGGACAAG TTTGACGCTA GCAAGAACGA CCTGGGAGGC 
TGCAATGGGA CATCAACCAT GACTGTAATC AAAGATTACT ATGCACTGAA GGAGAATGAA 
ATCTGCGTGA GCCAAGGTGA GGTGGTACAG GTCCTCGCCG TCAACCAGCA GAACATGTGT 
CTGGTGTACC AGCCTGCCAG CGACCATTCC CCGGCAGCAG AGGGCTGGGT CCCAGGCAGC 
ATCCTGGCGC CCCTCGCCAA AGCCACAGCA GCGGCCGAAA GTAGTGACGG GAGCATCAAG 
AAGTCATGCT CTTGGCACAC TCTACGCATG AGAAAGCGGG CAGACGTGGA GAACTCGGGT 
AAAAATGAAG CCACAGGGCC TCGTAAACCC AAGGATATTC TGGGCAACAA AGTCTCTGTT 
AAAGAGACGA ACAGTTCCGA GGAGTCAGAG TGTGATGATC TTGACCCTAA TACTAGTATG 
GAGATCTTGA ATCCAAATTT CATCCAGGAA GTGGCTCCAG AATTCCTCGT GCCCTTAGTA 
GATGTGACCT GTTTGCTTGG AGACACAGTG CTACTGCAGT GTAAAGCCTG TGGGCGGCCA 
AAGCCCAGCA TCACCTGGAA GGGTCCGGAT CAGAACATCC TAGACACTGA CAACAGTTCA 
GCAACATACA CCATCTCCTC CTGTGATTCT GGGGAAAGCA CCCTCAAGAT CTGCAACCTG 
ATGCCCCAGG ATAGTGGGAT TTATACCTGC ATAGCAGCAA ACGACCACGG GACTGCATCC 
ACATCTGCTA CAGTTAAGGT GCAAGGTGTC CCCGCAGCCC CCAACCGCCC AATCGCACAG 
GAGAGGAGCT GCACCTCCGT CATCCTCCGC TGGCTGCCCC CAGCCAGCAC CGGAAACTGC 
ACTATTTCGG GGTACACAGT GGAGTATCGC GAGGAAGGTT CCCAGGTCTG GCAGCAATCC 
GTAGCTTCGA CCTTGGACAC TTACCTGGTC ATTGAAGACC TTAGTCCTGG GTGCCCTTAT 
CAGTTCAGGG TCAGTGCCAG CAACCCCTGG GGAATCAGCC TTCCCAGTGA GCCCTCGGAG 
TTTGTGCGCC TTCCAGAGTA CGATGCAGCC GCCGACGGTG CCACCATTTC TTGGAAGGAA 
AATTTTGATT CTGCTTACAC TGAACTGAAC GAGATTGGCA GAGGCCGTTT CTCTATTGTA 
AAGAAATGCA TTCACAAAGC TACCCGCAAA GATGTTGCTG TGAAATTTGT GAGCAAAAAA 
ATGAAGAAGA AGGAGCAGGC TGCTCACGAG GCCGCCCTGC TCCAGCACCT GCAGCACCCT 
CAGTATGTCA CGCTCCATGA CACCTACGAG TCCCCCACAT CCTACATCCT GATTTTGGAA 
CTGATGGATG ACGGCCGACT CTTGGACTAC CTTATGAATC ATGATGAGCT GATGGAAGAA 
AAGGTAGCTT TCTACATTCG AGACATCATG GAAGCTCTGC AGTACCTGCA CAACTGTAGG 
GTCGCACATT TGGACATAAA GCCCGAAAAC CTGCTCATTG ACCTCCGGAT CCCAGTGCCT 
CGAGTGAAGC TCATCGACTT GGAAGACGCT GTCCAGATCT CAGGCCACTT CCATATCCAC 
CATCTGCTGG GGAACCCTGA GTTCGCTGCC CCCGAAGTGA TCCAAGGCAT CCCTGTCTCC 
CTGGGCACAG ACATCTGGAG CATCGGGGTT CTGACATATG TCATGCTTAG TGGGGTCTCC 
CCCTTCTTAG ATGAGAGCAA AGAGGAAACC TGTATCAACG TGTGCAGGGT GGACTTTAGC 
TTCCCCCACG AGTACTTCTG CGGTGTGAGC AATGCTGCCA GGGATTTCAT CAACGTAATC 
TTACAGGAAG ACTTCCGGAG GCGGCCCACA GCAGCCACGT GCCTGCAGCA TCCCTGGCTA 
CAGCCCCACA ACGGCAGCTA CTCCAAGATC CCCTTGGACA CCTCCCGTCT GGCCTGCTTC 
ATAGAACGCC GCAAGCACCA GAATGATGTG CGGCCCATTC CCAATGTCAA GAGTTACATC 
GTCAATAGGG TGAACCAAGG GACCTCGCTA TCCCACAACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317077.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172566.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X38, mRNA.

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CloneID	OMu79375
Clone ID Related Accession (Same CDS sequence)	XM_017317077.1 , XM_017317077.2
Accession Version	XM_017317077.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317077.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGTCGTCT 
TTGCTAGCAG CCCGGCAGGC TCCCCCTGAC GTGCCTACTG CTGCAGACCT TGTCAGTGCA 
ATAGAAAAGT TGGTCAAAAA TAAGCTGACT CTGGAAGGAG GCTCTTACCG GGGGAGCTTG 
AAGGACCCTA CAGGCTGCCT GAATGAGGGG ATGACCCCTC CCACACCTCC TAGAAACCTG 
GAAGAAGAAC AGAAAGCCAA GGCGCTGAGA GGCAGGATGT TTGTCCTGAA TGAGCTGGTA 
CAGACAGAGA AGGACTACGT CAAGGACCTG GGGATTGTGG TGGAGGGCTT CATGAAGAGA 
ATAGAAGAGA AGGGGGTCCC TGAGGACATG CGAGGGAAGG AAAAGATCGT GTTTGGAAAT 
ATCCACCAGA TTTACGACTG GCATAAAGAT TTTTTCCTGG CTGAACTGGA AAAATGTATC 
CAAGAGCAAG ACAGATTGGC CCAGCTCTTC ATTAAACACG AGCGGAAGCT GCACATCTAC 
GTGTGGTACT GCCAGAACAA GCCGCGTTCC GAGTACATCG TGGCTGAGTA CGATGCCTAT 
TTTGAGGAGG TTAAACAAGA GATCAATCAA AGGCTGACCC TTAGTGACTT CCTGATCAAG 
CCCATTCAGA GAATAACAAA GTACCAGCTG CTACTCAAGG ACTTCCTGCG ATACAGCGAG 
AAGGCTGGTT TGGAGTGTTC CGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTCCCC 
AAGCGCTGTA ATGACATGAT GAATCTGGGA CGCCTGCAGG GCTTCGAGGG CACCCTGACC 
GCCCAGGGCA AGCTACTGCA GCAGGACACA TTCTATGTGA TCGAGCTGGA TGCCGGCATG 
CAGTCCCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AGATTGTCAT CTTCAGTGAA 
CTGCTCCGAA AGGGGTCCCT CACCCCAGGC TACATGTTCA AAAGAAGCAT CAAGATGAAT 
TACTTGGTCC TGGAAGACAA TGTGGATGGT GACCCCTGCA AATTTGCACT CATGAACAGG 
GAGACTTCAG AAAGGGTCAT TCTACAGGCT GCCAACTCAG ACATCCAGCA GGCCTGGGTG 
CAGGACATCA ACCAAGTCCT AGAAACACAG AGGGACTTCT TAAATGCACT ACAGTCACCC 
ATTGAGTATC AGCGGAAGGA GAGAAGCACA GCTGTGATCC GGTCCCAGCC CCCGAGGGTT 
CCACAAGCCA GCCCCAGGCC CTACTCTTCT GGCCCGGTGG GCTCAGAGAA GCCCCCCAAG 
GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCTAA TGGCAGTCCA 
GGGTTCGACT ACCATCAGCC TGGGGACAAG TTTGACGCTA GCAAGAACGA CCTGGGAGGC 
TGCAATGGGA CATCAACCAT GACTGTAATC AAAGATTACT ATGCACTGAA GGAGAATGAA 
ATCTGCGTGA GCCAAGGTGA GGTGGTACAG GTCCTCGCCG TCAACCAGCA GAACATGTGT 
CTGGTGTACC AGCCTGCCAG CGACCATTCC CCGGCAGCAG AGGGCTGGGT CCCAGGCAGC 
ATCCTGGCGC CCCTCGCCAA AGCCACAGCA GCGGCCGAAA GTAGTGACGG GAGCATCAAG 
AAGTCATGCT CTTGGCACAC TCTACGCATG AGAAAGCGGG CAGACGTGGA GAACTCGGGT 
AAAAATGAAG CCACAGGGCC TCGTAAACCC AAGGATATTC TGGGCAACAA AGTCTCTGTT 
AAAGAGACGA ACAGTTCCGA GGAGTCAGAG TGTGATGATC TTGACCCTAA TACTAGTATG 
GAGATCTTGA ATCCAAATTT CATCCAGGAA GTGGCTCCAG AATTCCTCGT GCCCTTAGTA 
GATGTGACCT GTTTGCTTGG AGACACAGTG CTACTGCAGT GTAAAGCCTG TGGGCGGCCA 
AAGCCCAGCA TCACCTGGAA GGGTCCGGAT CAGAACATCC TAGACACTGA CAACAGTTCA 
GCAACATACA CCATCTCCTC CTGTGATTCT GGGGAAAGCA CCCTCAAGAT CTGCAACCTG 
ATGCCCCAGG ATAGTGGGAT TTATACCTGC ATAGCAGCAA ACGACCACGG GACTGCATCC 
ACATCTGCTA CAGTTAAGGT GCAAGGTGTC CCCGCAGCCC CCAACCGCCC AATCGCACAG 
GAGAGGAGCT GCACCTCCGT CATCCTCCGC TGGCTGCCCC CAGCCAGCAC CGGAAACTGC 
ACTATTTCGG GGTACACAGT GGAGTATCGC GAGGAAGGTT CCCAGGTCTG GCAGCAATCC 
GTAGCTTCGA CCTTGGACAC TTACCTGGTC ATTGAAGACC TTAGTCCTGG GTGCCCTTAT 
CAGTTCAGGG TCAGTGCCAG CAACCCCTGG GGAATCAGCC TTCCCAGTGA GCCCTCGGAG 
TTTGTGCGCC TTCCAGAGTA CGATGCAGCC GCCGACGGTG CCACCATTTC TTGGAAGGAA 
AATTTTGATT CTGCTTACAC TGAACTGAAC GAGATTGGCA GAGGCCGTTT CTCTATTGTA 
AAGAAATGCA TTCACAAAGC TACCCGCAAA GATGTTGCTG TGAAATTTGT GAGCAAAAAA 
ATGAAGAAGA AGGAGCAGGC TGCTCACGAG GCCGCCCTGC TCCAGCACCT GCAGCACCCT 
CAGTATGTCA CGCTCCATGA CACCTACGAG TCCCCCACAT CCTACATCCT GATTTTGGAA 
CTGATGGATG ACGGCCGACT CTTGGACTAC CTTATGAATC ATGATGAGCT GATGGAAGAA 
AAGGTAGCTT TCTACATTCG AGACATCATG GAAGCTCTGC AGTACCTGCA CAACTGTAGG 
GTCGCACATT TGGACATAAA GCCCGAAAAC CTGCTCATTG ACCTCCGGAT CCCAGTGCCT 
CGAGTGAAGC TCATCGACTT GGAAGACGCT GTCCAGATCT CAGGCCACTT CCATATCCAC 
CATCTGCTGG GGAACCCTGA GTTCGCTGCC CCCGAAGTGA TCCAAGGCAT CCCTGTCTCC 
CTGGGCACAG ACATCTGGAG CATCGGGGTT CTGACATATG TCATGCTTAG TGGGGTCTCC 
CCCTTCTTAG ATGAGAGCAA AGAGGAAACC TGTATCAACG TGTGCAGGGT GGACTTTAGC 
TTCCCCCACG AGTACTTCTG CGGTGTGAGC AATGCTGCCA GGGATTTCAT CAACGTAATC 
TTACAGGAAG ACTTCCGGAG GCGGCCCACA GCAGCCACGT GCCTGCAGCA TCCCTGGCTA 
CAGCCCCACA ACGGCAGCTA CTCCAAGATC CCCTTGGACA CCTCCCGTCT GGCCTGCTTC 
ATAGAACGCC GCAAGCACCA GAATGATGTG CGGCCCATTC CCAATGTCAA GAGTTACATC 
GTCAATAGGG TGAACCAAGG GACCTCGCTA TCCCACAACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172566.1
CDS	1521..5405
Translation	MKGGDQAYTRGPSLGWLFAKCCCCFPCRDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_017317077.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317077.2

ORF Insert Sequence:

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGTCGTCT 
TTGCTAGCAG CCCGGCAGGC TCCCCCTGAC GTGCCTACTG CTGCAGACCT TGTCAGTGCA 
ATAGAAAAGT TGGTCAAAAA TAAGCTGACT CTGGAAGGAG GCTCTTACCG GGGGAGCTTG 
AAGGACCCTA CAGGCTGCCT GAATGAGGGG ATGACCCCTC CCACACCTCC TAGAAACCTG 
GAAGAAGAAC AGAAAGCCAA GGCGCTGAGA GGCAGGATGT TTGTCCTGAA TGAGCTGGTA 
CAGACAGAGA AGGACTACGT CAAGGACCTG GGGATTGTGG TGGAGGGCTT CATGAAGAGA 
ATAGAAGAGA AGGGGGTCCC TGAGGACATG CGAGGGAAGG AAAAGATCGT GTTTGGAAAT 
ATCCACCAGA TTTACGACTG GCATAAAGAT TTTTTCCTGG CTGAACTGGA AAAATGTATC 
CAAGAGCAAG ACAGATTGGC CCAGCTCTTC ATTAAACACG AGCGGAAGCT GCACATCTAC 
GTGTGGTACT GCCAGAACAA GCCGCGTTCC GAGTACATCG TGGCTGAGTA CGATGCCTAT 
TTTGAGGAGG TTAAACAAGA GATCAATCAA AGGCTGACCC TTAGTGACTT CCTGATCAAG 
CCCATTCAGA GAATAACAAA GTACCAGCTG CTACTCAAGG ACTTCCTGCG ATACAGCGAG 
AAGGCTGGTT TGGAGTGTTC CGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTCCCC 
AAGCGCTGTA ATGACATGAT GAATCTGGGA CGCCTGCAGG GCTTCGAGGG CACCCTGACC 
GCCCAGGGCA AGCTACTGCA GCAGGACACA TTCTATGTGA TCGAGCTGGA TGCCGGCATG 
CAGTCCCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AGATTGTCAT CTTCAGTGAA 
CTGCTCCGAA AGGGGTCCCT CACCCCAGGC TACATGTTCA AAAGAAGCAT CAAGATGAAT 
TACTTGGTCC TGGAAGACAA TGTGGATGGT GACCCCTGCA AATTTGCACT CATGAACAGG 
GAGACTTCAG AAAGGGTCAT TCTACAGGCT GCCAACTCAG ACATCCAGCA GGCCTGGGTG 
CAGGACATCA ACCAAGTCCT AGAAACACAG AGGGACTTCT TAAATGCACT ACAGTCACCC 
ATTGAGTATC AGCGGAAGGA GAGAAGCACA GCTGTGATCC GGTCCCAGCC CCCGAGGGTT 
CCACAAGCCA GCCCCAGGCC CTACTCTTCT GGCCCGGTGG GCTCAGAGAA GCCCCCCAAG 
GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCTAA TGGCAGTCCA 
GGGTTCGACT ACCATCAGCC TGGGGACAAG TTTGACGCTA GCAAGAACGA CCTGGGAGGC 
TGCAATGGGA CATCAACCAT GACTGTAATC AAAGATTACT ATGCACTGAA GGAGAATGAA 
ATCTGCGTGA GCCAAGGTGA GGTGGTACAG GTCCTCGCCG TCAACCAGCA GAACATGTGT 
CTGGTGTACC AGCCTGCCAG CGACCATTCC CCGGCAGCAG AGGGCTGGGT CCCAGGCAGC 
ATCCTGGCGC CCCTCGCCAA AGCCACAGCA GCGGCCGAAA GTAGTGACGG GAGCATCAAG 
AAGTCATGCT CTTGGCACAC TCTACGCATG AGAAAGCGGG CAGACGTGGA GAACTCGGGT 
AAAAATGAAG CCACAGGGCC TCGTAAACCC AAGGATATTC TGGGCAACAA AGTCTCTGTT 
AAAGAGACGA ACAGTTCCGA GGAGTCAGAG TGTGATGATC TTGACCCTAA TACTAGTATG 
GAGATCTTGA ATCCAAATTT CATCCAGGAA GTGGCTCCAG AATTCCTCGT GCCCTTAGTA 
GATGTGACCT GTTTGCTTGG AGACACAGTG CTACTGCAGT GTAAAGCCTG TGGGCGGCCA 
AAGCCCAGCA TCACCTGGAA GGGTCCGGAT CAGAACATCC TAGACACTGA CAACAGTTCA 
GCAACATACA CCATCTCCTC CTGTGATTCT GGGGAAAGCA CCCTCAAGAT CTGCAACCTG 
ATGCCCCAGG ATAGTGGGAT TTATACCTGC ATAGCAGCAA ACGACCACGG GACTGCATCC 
ACATCTGCTA CAGTTAAGGT GCAAGGTGTC CCCGCAGCCC CCAACCGCCC AATCGCACAG 
GAGAGGAGCT GCACCTCCGT CATCCTCCGC TGGCTGCCCC CAGCCAGCAC CGGAAACTGC 
ACTATTTCGG GGTACACAGT GGAGTATCGC GAGGAAGGTT CCCAGGTCTG GCAGCAATCC 
GTAGCTTCGA CCTTGGACAC TTACCTGGTC ATTGAAGACC TTAGTCCTGG GTGCCCTTAT 
CAGTTCAGGG TCAGTGCCAG CAACCCCTGG GGAATCAGCC TTCCCAGTGA GCCCTCGGAG 
TTTGTGCGCC TTCCAGAGTA CGATGCAGCC GCCGACGGTG CCACCATTTC TTGGAAGGAA 
AATTTTGATT CTGCTTACAC TGAACTGAAC GAGATTGGCA GAGGCCGTTT CTCTATTGTA 
AAGAAATGCA TTCACAAAGC TACCCGCAAA GATGTTGCTG TGAAATTTGT GAGCAAAAAA 
ATGAAGAAGA AGGAGCAGGC TGCTCACGAG GCCGCCCTGC TCCAGCACCT GCAGCACCCT 
CAGTATGTCA CGCTCCATGA CACCTACGAG TCCCCCACAT CCTACATCCT GATTTTGGAA 
CTGATGGATG ACGGCCGACT CTTGGACTAC CTTATGAATC ATGATGAGCT GATGGAAGAA 
AAGGTAGCTT TCTACATTCG AGACATCATG GAAGCTCTGC AGTACCTGCA CAACTGTAGG 
GTCGCACATT TGGACATAAA GCCCGAAAAC CTGCTCATTG ACCTCCGGAT CCCAGTGCCT 
CGAGTGAAGC TCATCGACTT GGAAGACGCT GTCCAGATCT CAGGCCACTT CCATATCCAC 
CATCTGCTGG GGAACCCTGA GTTCGCTGCC CCCGAAGTGA TCCAAGGCAT CCCTGTCTCC 
CTGGGCACAG ACATCTGGAG CATCGGGGTT CTGACATATG TCATGCTTAG TGGGGTCTCC 
CCCTTCTTAG ATGAGAGCAA AGAGGAAACC TGTATCAACG TGTGCAGGGT GGACTTTAGC 
TTCCCCCACG AGTACTTCTG CGGTGTGAGC AATGCTGCCA GGGATTTCAT CAACGTAATC 
TTACAGGAAG ACTTCCGGAG GCGGCCCACA GCAGCCACGT GCCTGCAGCA TCCCTGGCTA 
CAGCCCCACA ACGGCAGCTA CTCCAAGATC CCCTTGGACA CCTCCCGTCT GGCCTGCTTC 
ATAGAACGCC GCAAGCACCA GAATGATGTG CGGCCCATTC CCAATGTCAA GAGTTACATC 
GTCAATAGGG TGAACCAAGG GACCTCGCTA TCCCACAACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522385.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522448.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X34, mRNA.

Price & Availability↑

CloneID	OMu52301
Clone ID Related Accession (Same CDS sequence)	XM_006522385.4 , XM_006522385.3
Accession Version	XM_006522385.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522385.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGTCGTCT 
TTGCTAGCAG CCCGGCAGGC TCCCCCTGAC GTGCCTACTG CTGCAGACCT TGTCAGTGCA 
ATAGAAAAGT TGGTCAAAAA TAAGCTGACT CTGGAAGGAG GCTCTTACCG GGGGAGCTTG 
AAGGACCCTA CAGGCTGCCT GAATGAGGGG ATGACCCCTC CCACACCTCC TAGAAACCTG 
GAAGAAGAAC AGAAAGCCAA GGCGCTGAGA GGCAGGATGT TTGTCCTGAA TGAGCTGGTA 
CAGACAGAGA AGGACTACGT CAAGGACCTG GGGATTGTGG TGGAGGGCTT CATGAAGAGA 
ATAGAAGAGA AGGGGGTCCC TGAGGACATG CGAGGGAAGG AAAAGATCGT GTTTGGAAAT 
ATCCACCAGA TTTACGACTG GCATAAAGAT TTTTTCCTGG CTGAACTGGA AAAATGTATC 
CAAGAGCAAG ACAGATTGGC CCAGCTCTTC ATTAAACACG AGCGGAAGCT GCACATCTAC 
GTGTGGTACT GCCAGAACAA GCCGCGTTCC GAGTACATCG TGGCTGAGTA CGATGCCTAT 
TTTGAGGAGG TTAAACAAGA GATCAATCAA AGGCTGACCC TTAGTGACTT CCTGATCAAG 
CCCATTCAGA GAATAACAAA GTACCAGCTG CTACTCAAGG ACTTCCTGCG ATACAGCGAG 
AAGGCTGGTT TGGAGTGTTC CGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTCCCC 
AAGCGCTGTA ATGACATGAT GAATCTGGGA CGCCTGCAGG GCTTCGAGGG CACCCTGACC 
GCCCAGGGCA AGCTACTGCA GCAGGACACA TTCTATGTGA TCGAGCTGGA TGCCGGCATG 
CAGTCCCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AGATTGTCAT CTTCAGTGAA 
CTGCTCCGAA AGGGGTCCCT CACCCCAGGC TACATGTTCA AAAGAAGCAT CAAGATGAAT 
TACTTGGTCC TGGAAGACAA TGTGGATGGT GACCCCTGCA AATTTGCACT CATGAACAGG 
GAGACTTCAG AAAGGGTCAT TCTACAGGCT GCCAACTCAG ACATCCAGCA GGCCTGGGTG 
CAGGACATCA ACCAAGTCCT AGAAACACAG AGGGACTTCT TAAATGCACT ACAGTCACCC 
ATTGAGTATC AGCGGAAGGA GAGAAGCACA GCTGTGATCC GGTCCCAGCC CCCGAGGGTT 
CCACAAGCCA GCCCCAGGCC CTACTCTTCT GGCCCGGTGG GCTCAGAGAA GCCCCCCAAG 
GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCTAA TGGCAGTCCA 
GGGTTCGACT ACCATCAGCC TGGGGACAAG TTTGACGCTA GCAAGCAGAA CGACCTGGGA 
GGCTGCAATG GGACATCAAC CATGACTGTA ATCAAAGATT ACTATGCACT GAAGGAGAAT 
GAAATCTGCG TGAGCCAAGG TGAGGTGGTA CAGGTCCTCG CCGTCAACCA GCAGAACATG 
TGTCTGGTGT ACCAGCCTGC CAGCGACCAT TCCCCGGCAG CAGAGGGCTG GGTCCCAGGC 
AGCATCCTGG CGCCCCTCGC CAAAGCCACA GCAGCGGCCG AAAGTAGTGA CGGGAGCATC 
AAGAAGTCAT GCTCTTGGCA CACTCTACGC ATGAGAAAGC GGGCAGACGT GGAGAACTCG 
GGTAAAAATG AAGCCACAGG GCCTCGTAAA CCCAAGGATA TTCTGGGCAA CAAAGTCTCT 
GTTAAAGAGA CGAACAGTTC CGAGGAGTCA GAGTGTGATG ATCTTGACCC TAATACTAGT 
ATGGAGATCT TGAATCCAAA TTTCATCCAG GAAGTGGCTC CAGAATTCCT CGTGCCCTTA 
GTAGATGTGA CCTGTTTGCT TGGAGACACA GTGCTACTGC AGTGTAAAGC CTGTGGGCGG 
CCAAAGCCCA GCATCACCTG GAAGGGTCCG GATCAGAACA TCCTAGACAC TGACAACAGT 
TCAGCAACAT ACACCATCTC CTCCTGTGAT TCTGGGGAAA GCACCCTCAA GATCTGCAAC 
CTGATGCCCC AGGATAGTGG GATTTATACC TGCATAGCAG CAAACGACCA CGGGACTGCA 
TCCACATCTG CTACAGTTAA GGTGCAAGGT GTCCCCGCAG CCCCCAACCG CCCAATCGCA 
CAGGAGAGGA GCTGCACCTC CGTCATCCTC CGCTGGCTGC CCCCAGCCAG CACCGGAAAC 
TGCACTATTT CGGGGTACAC AGTGGAGTAT CGCGAGGAAG GTTCCCAGGT CTGGCAGCAA 
TCCGTAGCTT CGACCTTGGA CACTTACCTG GTCATTGAAG ACCTTAGTCC TGGGTGCCCT 
TATCAGTTCA GGGTCAGTGC CAGCAACCCC TGGGGAATCA GCCTTCCCAG TGAGCCCTCG 
GAGTTTGTGC GCCTTCCAGA GTACGATGCA GCCGCCGACG GTGCCACCAT TTCTTGGAAG 
GAAAATTTTG ATTCTGCTTA CACTGAACTG AACGAGATTG GCAGAGGCCG TTTCTCTATT 
GTAAAGAAAT GCATTCACAA AGCTACCCGC AAAGATGTTG CTGTGAAATT TGTGAGCAAA 
AAAATGAAGA AGAAGGAGCA GGCTGCTCAC GAGGCCGCCC TGCTCCAGCA CCTGCAGCAC 
CCTCAGTATG TCACGCTCCA TGACACCTAC GAGTCCCCCA CATCCTACAT CCTGATTTTG 
GAACTGATGG ATGACGGCCG ACTCTTGGAC TACCTTATGA ATCATGATGA GCTGATGGAA 
GAAAAGGTAG CTTTCTACAT TCGAGACATC ATGGAAGCTC TGCAGTACCT GCACAACTGT 
AGGGTCGCAC ATTTGGACAT AAAGCCCGAA AACCTGCTCA TTGACCTCCG GATCCCAGTG 
CCTCGAGTGA AGCTCATCGA CTTGGAAGAC GCTGTCCAGA TCTCAGGCCA CTTCCATATC 
CACCATCTGC TGGGGAACCC TGAGTTCGCT GCCCCCGAAG TGATCCAAGG CATCCCTGTC 
TCCCTGGGCA CAGACATCTG GAGCATCGGG GTTCTGACAT ATGTCATGCT TAGTGGGGTC 
TCCCCCTTCT TAGATGAGAG CAAAGAGGAA ACCTGTATCA ACGTGTGCAG GGTGGACTTT 
AGCTTCCCCC ACGAGTACTT CTGCGGTGTG AGCAATGCTG CCAGGGATTT CATCAACGTA 
ATCTTACAGG AAGACTTCCG GAGGCGGCCC ACAGCAGCCA CGTGCCTGCA GCATCCCTGG 
CTACAGCCCC ACAACGGCAG CTACTCCAAG ATCCCCTTGG ACACCTCCCG TCTGGCCTGC 
TTCATAGAAC GCCGCAAGCA CCAGAATGAT GTGCGGCCCA TTCCCAATGT CAAGAGTTAC 
ATCGTCAATA GGGTGAACCA AGGGACCTCG CTATCCCACA ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522448.1
CDS	1578..5465
Translation	MKGGDQAYTRGPSLGWLFAKCCCCFPCRDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_006522385.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522385.3

ORF Insert Sequence:

ATGAAGGGTG GCGATCAGGC TTACACCCGA GGTCCCTCTT TGGGCTGGCT CTTTGCTAAG 
TGCTGCTGTT GCTTCCCTTG TAGAGATTCA TACTCTCATT CCTCCAGTGA GAATGGAGGC 
AAATCCGAGT CCGTGGCCCA CCTGCAGTCT CAGCCCTCCC TGAACTCCAT CCACAGTTCC 
CCTGGTCCCA AGCGCTCCAC CAACACCCTC AAAAAGTGGC TGACCAGCCC CGTGCGCCGC 
CTCAACAGCG GAAAAGCCGA CGGGAACATC AAAAAGCAGA AGAAAGTCCG GGATGGGCGG 
AAGAGCTTCG ATCTGGGATC GCCCAAGCCT GGGGATGAGA CGACACCTCA GGGAGACAGC 
GCTGACGAGA AGAGCAAGAA GGGTTGGGGC GAAGACGAGC CAGATGAAGA GTCACACACA 
CCCCTCCCTC CGCCCATGAA GATCTTTGAC AACGACCCCA CACAGGATGA GATGTCGTCT 
TTGCTAGCAG CCCGGCAGGC TCCCCCTGAC GTGCCTACTG CTGCAGACCT TGTCAGTGCA 
ATAGAAAAGT TGGTCAAAAA TAAGCTGACT CTGGAAGGAG GCTCTTACCG GGGGAGCTTG 
AAGGACCCTA CAGGCTGCCT GAATGAGGGG ATGACCCCTC CCACACCTCC TAGAAACCTG 
GAAGAAGAAC AGAAAGCCAA GGCGCTGAGA GGCAGGATGT TTGTCCTGAA TGAGCTGGTA 
CAGACAGAGA AGGACTACGT CAAGGACCTG GGGATTGTGG TGGAGGGCTT CATGAAGAGA 
ATAGAAGAGA AGGGGGTCCC TGAGGACATG CGAGGGAAGG AAAAGATCGT GTTTGGAAAT 
ATCCACCAGA TTTACGACTG GCATAAAGAT TTTTTCCTGG CTGAACTGGA AAAATGTATC 
CAAGAGCAAG ACAGATTGGC CCAGCTCTTC ATTAAACACG AGCGGAAGCT GCACATCTAC 
GTGTGGTACT GCCAGAACAA GCCGCGTTCC GAGTACATCG TGGCTGAGTA CGATGCCTAT 
TTTGAGGAGG TTAAACAAGA GATCAATCAA AGGCTGACCC TTAGTGACTT CCTGATCAAG 
CCCATTCAGA GAATAACAAA GTACCAGCTG CTACTCAAGG ACTTCCTGCG ATACAGCGAG 
AAGGCTGGTT TGGAGTGTTC CGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTCCCC 
AAGCGCTGTA ATGACATGAT GAATCTGGGA CGCCTGCAGG GCTTCGAGGG CACCCTGACC 
GCCCAGGGCA AGCTACTGCA GCAGGACACA TTCTATGTGA TCGAGCTGGA TGCCGGCATG 
CAGTCCCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AGATTGTCAT CTTCAGTGAA 
CTGCTCCGAA AGGGGTCCCT CACCCCAGGC TACATGTTCA AAAGAAGCAT CAAGATGAAT 
TACTTGGTCC TGGAAGACAA TGTGGATGGT GACCCCTGCA AATTTGCACT CATGAACAGG 
GAGACTTCAG AAAGGGTCAT TCTACAGGCT GCCAACTCAG ACATCCAGCA GGCCTGGGTG 
CAGGACATCA ACCAAGTCCT AGAAACACAG AGGGACTTCT TAAATGCACT ACAGTCACCC 
ATTGAGTATC AGCGGAAGGA GAGAAGCACA GCTGTGATCC GGTCCCAGCC CCCGAGGGTT 
CCACAAGCCA GCCCCAGGCC CTACTCTTCT GGCCCGGTGG GCTCAGAGAA GCCCCCCAAG 
GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCTAA TGGCAGTCCA 
GGGTTCGACT ACCATCAGCC TGGGGACAAG TTTGACGCTA GCAAGCAGAA CGACCTGGGA 
GGCTGCAATG GGACATCAAC CATGACTGTA ATCAAAGATT ACTATGCACT GAAGGAGAAT 
GAAATCTGCG TGAGCCAAGG TGAGGTGGTA CAGGTCCTCG CCGTCAACCA GCAGAACATG 
TGTCTGGTGT ACCAGCCTGC CAGCGACCAT TCCCCGGCAG CAGAGGGCTG GGTCCCAGGC 
AGCATCCTGG CGCCCCTCGC CAAAGCCACA GCAGCGGCCG AAAGTAGTGA CGGGAGCATC 
AAGAAGTCAT GCTCTTGGCA CACTCTACGC ATGAGAAAGC GGGCAGACGT GGAGAACTCG 
GGTAAAAATG AAGCCACAGG GCCTCGTAAA CCCAAGGATA TTCTGGGCAA CAAAGTCTCT 
GTTAAAGAGA CGAACAGTTC CGAGGAGTCA GAGTGTGATG ATCTTGACCC TAATACTAGT 
ATGGAGATCT TGAATCCAAA TTTCATCCAG GAAGTGGCTC CAGAATTCCT CGTGCCCTTA 
GTAGATGTGA CCTGTTTGCT TGGAGACACA GTGCTACTGC AGTGTAAAGC CTGTGGGCGG 
CCAAAGCCCA GCATCACCTG GAAGGGTCCG GATCAGAACA TCCTAGACAC TGACAACAGT 
TCAGCAACAT ACACCATCTC CTCCTGTGAT TCTGGGGAAA GCACCCTCAA GATCTGCAAC 
CTGATGCCCC AGGATAGTGG GATTTATACC TGCATAGCAG CAAACGACCA CGGGACTGCA 
TCCACATCTG CTACAGTTAA GGTGCAAGGT GTCCCCGCAG CCCCCAACCG CCCAATCGCA 
CAGGAGAGGA GCTGCACCTC CGTCATCCTC CGCTGGCTGC CCCCAGCCAG CACCGGAAAC 
TGCACTATTT CGGGGTACAC AGTGGAGTAT CGCGAGGAAG GTTCCCAGGT CTGGCAGCAA 
TCCGTAGCTT CGACCTTGGA CACTTACCTG GTCATTGAAG ACCTTAGTCC TGGGTGCCCT 
TATCAGTTCA GGGTCAGTGC CAGCAACCCC TGGGGAATCA GCCTTCCCAG TGAGCCCTCG 
GAGTTTGTGC GCCTTCCAGA GTACGATGCA GCCGCCGACG GTGCCACCAT TTCTTGGAAG 
GAAAATTTTG ATTCTGCTTA CACTGAACTG AACGAGATTG GCAGAGGCCG TTTCTCTATT 
GTAAAGAAAT GCATTCACAA AGCTACCCGC AAAGATGTTG CTGTGAAATT TGTGAGCAAA 
AAAATGAAGA AGAAGGAGCA GGCTGCTCAC GAGGCCGCCC TGCTCCAGCA CCTGCAGCAC 
CCTCAGTATG TCACGCTCCA TGACACCTAC GAGTCCCCCA CATCCTACAT CCTGATTTTG 
GAACTGATGG ATGACGGCCG ACTCTTGGAC TACCTTATGA ATCATGATGA GCTGATGGAA 
GAAAAGGTAG CTTTCTACAT TCGAGACATC ATGGAAGCTC TGCAGTACCT GCACAACTGT 
AGGGTCGCAC ATTTGGACAT AAAGCCCGAA AACCTGCTCA TTGACCTCCG GATCCCAGTG 
CCTCGAGTGA AGCTCATCGA CTTGGAAGAC GCTGTCCAGA TCTCAGGCCA CTTCCATATC 
CACCATCTGC TGGGGAACCC TGAGTTCGCT GCCCCCGAAG TGATCCAAGG CATCCCTGTC 
TCCCTGGGCA CAGACATCTG GAGCATCGGG GTTCTGACAT ATGTCATGCT TAGTGGGGTC 
TCCCCCTTCT TAGATGAGAG CAAAGAGGAA ACCTGTATCA ACGTGTGCAG GGTGGACTTT 
AGCTTCCCCC ACGAGTACTT CTGCGGTGTG AGCAATGCTG CCAGGGATTT CATCAACGTA 
ATCTTACAGG AAGACTTCCG GAGGCGGCCC ACAGCAGCCA CGTGCCTGCA GCATCCCTGG 
CTACAGCCCC ACAACGGCAG CTACTCCAAG ATCCCCTTGG ACACCTCCCG TCTGGCCTGC 
TTCATAGAAC GCCGCAAGCA CCAGAATGAT GTGCGGCCCA TTCCCAATGT CAAGAGTTAC 
ATCGTCAATA GGGTGAACCA AGGGACCTCG CTATCCCACA ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522382.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522445.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OMu52298
Clone ID Related Accession (Same CDS sequence)	XM_006522382.3 , XM_006522382.4
Accession Version	XM_006522382.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522382.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGAT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC TATCCTAAAG 
GAGAAAGTGG CCTTTGTGTC TGGGGGGCGT GACAAGCGAG GTGGACCCAT CCTGACCTTC 
CCTGCCCGAA GCAATCATGA CAGAATAAGA CAGGAAGACC TACGGAAACT TGTGACGTAT 
TTGGCCAGCG TGCCAAGTGA GGACGTTTGC AAACGTGGCT TCACCGTCAT CATCGACATG 
CGGGGCTCCA AGTGGGATCT CATCAAGCCC CTCCTCAAGA CGCTGCAGGA AGCCTTCCCC 
GCCGAGATCC ATGTGGCCCT CATCATCAAG CCTGACAACT TCTGGCAGAA GCAGAAGACC 
AACTTTGGCA GCTCCAAATT CATCTTTGAG ACAAGCATGG TGTCTGTGGA GGGCCTCACG 
AAGCTGGTGG ACCCCTCCCA GCTGACAGAG GAGTTTGACG GCTCCCTGGA TTACAACCAT 
GAGGAGTGGA TTGAGTTGCG CCTCTCCCTG GAGGAGTTCT TCAACAGTGC GGTCCACCTG 
CTCTCACGCC TTGAGGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC TGTGGATGTG 
GAGGGCTCTC GGAGGCTTAT CGACGAGCAC ACGCAACTTA AGAAAAAGGT GCTGAAGGCC 
CCCGTGGAAG AGCTGGACCG GGAGGGGCAG CGTCTACTGC AGTGCATCCG CTGCAGCGAT 
GGCTTCTCAG GGCGCAACTG CATCCCAGGA AGTGCCGACT TCCAGAGCCT TGTACCCAAG 
ATCACCAGCC TCCTGGACAA GCTGCATTCC ACCCGACAAC ACCTGCACCA AATGTGGCAC 
GTGCGAAAGC TCAAGCTGGA TCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAG 
AAGATGTTCG ACTGGATAAG CCACAACAAG GAGTTATTTC TTCAGAGCCA CACAGAGATC 
GGAGTCAGCT ACCAGCATGC ACTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC 
TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TCGCTTCCCG CCTCTCAGAG 
GCCGGCCATT ATGCCTCCCA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG 
AAGAGCTTTG CCGCTGCCCT GGATGAACGC AGTACAATCC TGGCCATGTC TGCTGTGTTC 
CACCAGAAGG CAGAGCAGTT CCTGTCAGGC GTGGATGCCT GGTGTAAGAT GTGCAGCGAA 
GGCGGCCTTC CTTCTGAGAT GCAGGACCTG GAGCTGGCAA TCCACCATCA CCAGTCCTTA 
TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ATGGCAAAGC ACTACTGGAT 
GTCCTGCAGC GTCCCCTGAG TCCTGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC 
TCCAAGGCGG TCCACCAGGT GCTGGATGTG GTCCACGAGG TGCTGCACCA TCAGCGACGG 
CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTTCACC AGCGGCTGCA GCTGTGTGTC 
TTCCAGCAGG ATGTGCAGCA GGTGCTGGAC TGGATTGAAA ACCATGGCGA GGCCTTTCTC 
AGCAAACACA CTGGGGTTGG CAAGTCCTTG CATCGAGCAC GTGCACTACA GAAGAGGCAT 
GATGACTTTG AAGAAGTGGC ACAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCA 
GCGGAGCAGC TGGCTCAGAC TGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCCCGG 
CACCTGGAGG TGCGCATCCA AGACTTTGTG CGCAGAGTGG AGCAGCGGAA GCTGCTTCTG 
GACATGTCGG TCTCTTTCCA CACGCACACC AAAGAGCTGT GGACATGGAT GGAGGACCTT 
CAGAAGGAGG TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAGCTGATC 
AAGCAGTTCC AGCAGCAGCA GACCGCCACC CTGGATGCCA CTCTCAATGT CATCAAGGAA 
GGCGAAGACC TTATCCAGCA GCTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCACCGAG 
GCCAGGGACT CAGCGATGTC CAACAACAAG ACACCCCACA GTAGCTCTAT CAGCCATATT 
GAGTCTGTCC TCCAGCAGCT GGACGATGCT CAGGTACAGA TGGAAGAGCT GTTTCACGAG 
CGGAAGATCA AGCTGGACAT CTTCCTACAG CTGCGCATCT TTGAACAGTA CACCATTGAG 
GTCACTGCAG AGCTGGATGC CTGGAACGAG GACTTGCTCC GGCAGATGAA TGATTTCAAC 
ACAGAGGATC TGACCCTGGC TGAGCAGCGG CTACAGCGCC ACACAGAACG GAAGCTTGCC 
ATGAACAACA TGACGTTCGA GGTCATCCAG CAGGGCCAGG ACCTGCACCA GTACATCATG 
GAAGTGCAAG CGTCAGGAAT CGAGTTAATC TGTGAGAAGG ACCTTGACCT GGCAGCTCAG 
GTGCAGGAGC TGCTGGAGTT TCTCCATGAG AAGCAGCACG AGCTGGAGCT CAATGCGGAG 
CAGACGCACA AGCGGCTAGA GCAGTGTCTC CAGCTGAGGC ACCTCCAGGC TGAGGTCAAA 
CAGGTTCTGG GATGGATCCG CAATGGAGAG TCGATGCTCA ACGCCAGCCT GGTCAACGCC 
AGCTCTTTGT CGGAGGCAGA GCAGCTGCAG CGGGAGCATG AACAGTTCCA GCTAGCCATC 
GAGTCCCTCT TTCATGCCAC TTCCTTGCAG AAGACGCACC AGAGCGCCCT GCAGGTACAG 
CAGAAGGCAG AGGCACTGCT CCAGGCCGGC CACTACGACG CAGACGCCAT CCGGGAATGT 
GCTGAGAAGG TGGCCCTCCA CTGGCAACAG CTCATGCTAA AGATGGAGGA CCGGCTGAAG 
CTTGTCAACG CCTCTGTGGC CTTTTACAAG ACCTCTGAGC AGGTGTGCAG TGTTCTGGAG 
AGCTTGGAGC AGGAGTACCG GAGAGACGAG GACTGGTGTG GTGGGAGAGA CAAGCTGGGG 
CCGGCGGCAG AGATGGACCA CGTTATTCCA CTCCTCAGCA AGCACCTGGA GCAGAAGGAG 
GCCTTCCTGA AGGCCTGCAC GCTGGCAAGG CGGAACGCCG AGGTGTTCCT CAAATACATC 
CACAGGAACA ATGTGAGCAT GCCTAGTGTT GCCAGCCACA CCCGGGGGCC TGAACAGCAA 
GTAAAAGCCA TCTTGAGCGA GCTCTTGCAG AGGGAGAATC GAGTCCTGCA CTTCTGGACC 
TTGAAGAAGC GGCGGTTAGA CCAGTGTCAG CAATATGTGG TGTTTGAGCG CAGTGCTAAG 
CAGGCACTGG ACTGGATCCA AGAAACAGGA GAATATTACC TCTCAACACA TACCTCCACC 
GGAGAGACCA CGGAGGAGAC TCAGGAGCTG CTGAAAGAGT ATGGAGAATT CAGGGTGCCT 
GCCAAGCAAA CGAAGGAGAA GGTGAAACTT CTGATCCAGC TAGCAGACAG CTTTGTGGAA 
AAGGGCCACA TCCACGCCAC AGAGATACGG AAATGGGTGA CCACGGTGGA CAAGCACTAC 
CGCGATTTCT CCCTGAGGAT GGGGAAGTAC CGGTACTCGC TGGAGAAGGC GCTGGGGGTC 
AACACGGAGG ATAACAAGGA CCTAGAGCTG GACATCATCC CAGCAAGCCT TTCGGATCGG 
GAGGTCAAGC TCCGGGACGC TAACCATGAG ATCAATGAAG AGAAACGGAA GTCAGCTCGA 
AAGAAAGAGT TCATTATGGC TGAACTGCTC CAAACTGAGA AGGCTTATGT AAGGGACTTG 
CACGAGTGCT TGGAGACCTA CCTGTGGGAG ATGACTAGTG GTGTGGAAGA GATACCCCCC 
GGCATCCTCA ATAAGGAACA TATCATCTTC GGCAACATCC AGGAGATCTA TGACTTCCAT 
AACAACATCT TCCTCAAAGA GCTGGAGAAG TACGAGCAGC TGCCTGAGGA TGTGGGACAC 
TGCTTTGTTA CCTGGGCAGA CAAATTTCAG ATGTATGTCA CCTACTGTAA AAACAAGCCT 
GATTCCAACC AGCTGATCCT GGAGCATGCG GGCACCTTCT TTGATGAGAT CCAGCAGCGG 
CATGGCCTGG CCAACTCCAT CTCCTCCTAC CTAATTAAGC CTGTCCAGAG GGTTACCAAG 
TACCAGCTGC TCCTGAAGGA ACTTCTAACT TGCTGTGAAG AAGGGAAAGG AGAGCTGAAG 
GATGGCCTGG AGGTGATGCT CAGTGTCCCC AAGAAAGCCA ACGATGCCAT GCATGTCAGC 
ATGCTGGAAG GGTTCGATGA AAACTTGGAT GTACAGGGGG AGTTGATTCT CCAGGATGCC 
TTTCAAGTGT GGGACCCAAA GTCGCTGATC CGGAAGGGGC GGGAGCGCCA TCTGTTCCTC 
TTTGAGATCT CCTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACGAAATAT 
GTTTACAAGA ACAAGCTACT GACCTCAGAG CTGGGTGTGA CCGAGCATGT AGAAGGTGAC 
CCCTGCAAAT TCGCCTTGTG GTCTGGGCGC ACCCCATCCT CAGACAATAA AACAGTGCTC 
AAAGCTTCCA ACATCGAAAC CAAGCAGGAA TGGATCAAGA ACATTCGAGA AGTGATTCAG 
GAGAGGATCA TTCATCTTAA AGGCGCTTTA AAGGAGCCAA TTCAGCTCCC CAAAACACCA 
GCCAAACTGA GGAACAATAG TAAGAGGGAT GGGGTAGAGG ATGGTGACAG CCAAGGGGAT 
GGGAGCAGCC AGCCAGATAC CATATCCATT GCCTCCAGGA CCTCTCAGAA CACAGTGGAA 
AGTGACAAGG ATGGCAACCT TGTTCCTCGT TGGCACCTGG GACCTGGAGA TCCTTTCTCC 
ACTTACGTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522445.1
CDS	3334..8325
Translation	MTDRFWDQWYLWYLRLLRLLDRGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKDGNLVPRWHLGPGDPFSTYV

Target ORF information:

RefSeq Version	XM_006522382.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522382.4

ORF Insert Sequence:

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGAT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC TATCCTAAAG 
GAGAAAGTGG CCTTTGTGTC TGGGGGGCGT GACAAGCGAG GTGGACCCAT CCTGACCTTC 
CCTGCCCGAA GCAATCATGA CAGAATAAGA CAGGAAGACC TACGGAAACT TGTGACGTAT 
TTGGCCAGCG TGCCAAGTGA GGACGTTTGC AAACGTGGCT TCACCGTCAT CATCGACATG 
CGGGGCTCCA AGTGGGATCT CATCAAGCCC CTCCTCAAGA CGCTGCAGGA AGCCTTCCCC 
GCCGAGATCC ATGTGGCCCT CATCATCAAG CCTGACAACT TCTGGCAGAA GCAGAAGACC 
AACTTTGGCA GCTCCAAATT CATCTTTGAG ACAAGCATGG TGTCTGTGGA GGGCCTCACG 
AAGCTGGTGG ACCCCTCCCA GCTGACAGAG GAGTTTGACG GCTCCCTGGA TTACAACCAT 
GAGGAGTGGA TTGAGTTGCG CCTCTCCCTG GAGGAGTTCT TCAACAGTGC GGTCCACCTG 
CTCTCACGCC TTGAGGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC TGTGGATGTG 
GAGGGCTCTC GGAGGCTTAT CGACGAGCAC ACGCAACTTA AGAAAAAGGT GCTGAAGGCC 
CCCGTGGAAG AGCTGGACCG GGAGGGGCAG CGTCTACTGC AGTGCATCCG CTGCAGCGAT 
GGCTTCTCAG GGCGCAACTG CATCCCAGGA AGTGCCGACT TCCAGAGCCT TGTACCCAAG 
ATCACCAGCC TCCTGGACAA GCTGCATTCC ACCCGACAAC ACCTGCACCA AATGTGGCAC 
GTGCGAAAGC TCAAGCTGGA TCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAG 
AAGATGTTCG ACTGGATAAG CCACAACAAG GAGTTATTTC TTCAGAGCCA CACAGAGATC 
GGAGTCAGCT ACCAGCATGC ACTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC 
TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TCGCTTCCCG CCTCTCAGAG 
GCCGGCCATT ATGCCTCCCA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG 
AAGAGCTTTG CCGCTGCCCT GGATGAACGC AGTACAATCC TGGCCATGTC TGCTGTGTTC 
CACCAGAAGG CAGAGCAGTT CCTGTCAGGC GTGGATGCCT GGTGTAAGAT GTGCAGCGAA 
GGCGGCCTTC CTTCTGAGAT GCAGGACCTG GAGCTGGCAA TCCACCATCA CCAGTCCTTA 
TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ATGGCAAAGC ACTACTGGAT 
GTCCTGCAGC GTCCCCTGAG TCCTGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC 
TCCAAGGCGG TCCACCAGGT GCTGGATGTG GTCCACGAGG TGCTGCACCA TCAGCGACGG 
CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTTCACC AGCGGCTGCA GCTGTGTGTC 
TTCCAGCAGG ATGTGCAGCA GGTGCTGGAC TGGATTGAAA ACCATGGCGA GGCCTTTCTC 
AGCAAACACA CTGGGGTTGG CAAGTCCTTG CATCGAGCAC GTGCACTACA GAAGAGGCAT 
GATGACTTTG AAGAAGTGGC ACAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCA 
GCGGAGCAGC TGGCTCAGAC TGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCCCGG 
CACCTGGAGG TGCGCATCCA AGACTTTGTG CGCAGAGTGG AGCAGCGGAA GCTGCTTCTG 
GACATGTCGG TCTCTTTCCA CACGCACACC AAAGAGCTGT GGACATGGAT GGAGGACCTT 
CAGAAGGAGG TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAGCTGATC 
AAGCAGTTCC AGCAGCAGCA GACCGCCACC CTGGATGCCA CTCTCAATGT CATCAAGGAA 
GGCGAAGACC TTATCCAGCA GCTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCACCGAG 
GCCAGGGACT CAGCGATGTC CAACAACAAG ACACCCCACA GTAGCTCTAT CAGCCATATT 
GAGTCTGTCC TCCAGCAGCT GGACGATGCT CAGGTACAGA TGGAAGAGCT GTTTCACGAG 
CGGAAGATCA AGCTGGACAT CTTCCTACAG CTGCGCATCT TTGAACAGTA CACCATTGAG 
GTCACTGCAG AGCTGGATGC CTGGAACGAG GACTTGCTCC GGCAGATGAA TGATTTCAAC 
ACAGAGGATC TGACCCTGGC TGAGCAGCGG CTACAGCGCC ACACAGAACG GAAGCTTGCC 
ATGAACAACA TGACGTTCGA GGTCATCCAG CAGGGCCAGG ACCTGCACCA GTACATCATG 
GAAGTGCAAG CGTCAGGAAT CGAGTTAATC TGTGAGAAGG ACCTTGACCT GGCAGCTCAG 
GTGCAGGAGC TGCTGGAGTT TCTCCATGAG AAGCAGCACG AGCTGGAGCT CAATGCGGAG 
CAGACGCACA AGCGGCTAGA GCAGTGTCTC CAGCTGAGGC ACCTCCAGGC TGAGGTCAAA 
CAGGTTCTGG GATGGATCCG CAATGGAGAG TCGATGCTCA ACGCCAGCCT GGTCAACGCC 
AGCTCTTTGT CGGAGGCAGA GCAGCTGCAG CGGGAGCATG AACAGTTCCA GCTAGCCATC 
GAGTCCCTCT TTCATGCCAC TTCCTTGCAG AAGACGCACC AGAGCGCCCT GCAGGTACAG 
CAGAAGGCAG AGGCACTGCT CCAGGCCGGC CACTACGACG CAGACGCCAT CCGGGAATGT 
GCTGAGAAGG TGGCCCTCCA CTGGCAACAG CTCATGCTAA AGATGGAGGA CCGGCTGAAG 
CTTGTCAACG CCTCTGTGGC CTTTTACAAG ACCTCTGAGC AGGTGTGCAG TGTTCTGGAG 
AGCTTGGAGC AGGAGTACCG GAGAGACGAG GACTGGTGTG GTGGGAGAGA CAAGCTGGGG 
CCGGCGGCAG AGATGGACCA CGTTATTCCA CTCCTCAGCA AGCACCTGGA GCAGAAGGAG 
GCCTTCCTGA AGGCCTGCAC GCTGGCAAGG CGGAACGCCG AGGTGTTCCT CAAATACATC 
CACAGGAACA ATGTGAGCAT GCCTAGTGTT GCCAGCCACA CCCGGGGGCC TGAACAGCAA 
GTAAAAGCCA TCTTGAGCGA GCTCTTGCAG AGGGAGAATC GAGTCCTGCA CTTCTGGACC 
TTGAAGAAGC GGCGGTTAGA CCAGTGTCAG CAATATGTGG TGTTTGAGCG CAGTGCTAAG 
CAGGCACTGG ACTGGATCCA AGAAACAGGA GAATATTACC TCTCAACACA TACCTCCACC 
GGAGAGACCA CGGAGGAGAC TCAGGAGCTG CTGAAAGAGT ATGGAGAATT CAGGGTGCCT 
GCCAAGCAAA CGAAGGAGAA GGTGAAACTT CTGATCCAGC TAGCAGACAG CTTTGTGGAA 
AAGGGCCACA TCCACGCCAC AGAGATACGG AAATGGGTGA CCACGGTGGA CAAGCACTAC 
CGCGATTTCT CCCTGAGGAT GGGGAAGTAC CGGTACTCGC TGGAGAAGGC GCTGGGGGTC 
AACACGGAGG ATAACAAGGA CCTAGAGCTG GACATCATCC CAGCAAGCCT TTCGGATCGG 
GAGGTCAAGC TCCGGGACGC TAACCATGAG ATCAATGAAG AGAAACGGAA GTCAGCTCGA 
AAGAAAGAGT TCATTATGGC TGAACTGCTC CAAACTGAGA AGGCTTATGT AAGGGACTTG 
CACGAGTGCT TGGAGACCTA CCTGTGGGAG ATGACTAGTG GTGTGGAAGA GATACCCCCC 
GGCATCCTCA ATAAGGAACA TATCATCTTC GGCAACATCC AGGAGATCTA TGACTTCCAT 
AACAACATCT TCCTCAAAGA GCTGGAGAAG TACGAGCAGC TGCCTGAGGA TGTGGGACAC 
TGCTTTGTTA CCTGGGCAGA CAAATTTCAG ATGTATGTCA CCTACTGTAA AAACAAGCCT 
GATTCCAACC AGCTGATCCT GGAGCATGCG GGCACCTTCT TTGATGAGAT CCAGCAGCGG 
CATGGCCTGG CCAACTCCAT CTCCTCCTAC CTAATTAAGC CTGTCCAGAG GGTTACCAAG 
TACCAGCTGC TCCTGAAGGA ACTTCTAACT TGCTGTGAAG AAGGGAAAGG AGAGCTGAAG 
GATGGCCTGG AGGTGATGCT CAGTGTCCCC AAGAAAGCCA ACGATGCCAT GCATGTCAGC 
ATGCTGGAAG GGTTCGATGA AAACTTGGAT GTACAGGGGG AGTTGATTCT CCAGGATGCC 
TTTCAAGTGT GGGACCCAAA GTCGCTGATC CGGAAGGGGC GGGAGCGCCA TCTGTTCCTC 
TTTGAGATCT CCTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACGAAATAT 
GTTTACAAGA ACAAGCTACT GACCTCAGAG CTGGGTGTGA CCGAGCATGT AGAAGGTGAC 
CCCTGCAAAT TCGCCTTGTG GTCTGGGCGC ACCCCATCCT CAGACAATAA AACAGTGCTC 
AAAGCTTCCA ACATCGAAAC CAAGCAGGAA TGGATCAAGA ACATTCGAGA AGTGATTCAG 
GAGAGGATCA TTCATCTTAA AGGCGCTTTA AAGGAGCCAA TTCAGCTCCC CAAAACACCA 
GCCAAACTGA GGAACAATAG TAAGAGGGAT GGGGTAGAGG ATGGTGACAG CCAAGGGGAT 
GGGAGCAGCC AGCCAGATAC CATATCCATT GCCTCCAGGA CCTCTCAGAA CACAGTGGAA 
AGTGACAAGG ATGGCAACCT TGTTCCTCGT TGGCACCTGG GACCTGGAGA TCCTTTCTCC 
ACTTACGTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522383.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522446.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X32, mRNA.

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CloneID	OMu52299
Clone ID Related Accession (Same CDS sequence)	XM_006522383.4 , XM_006522383.3
Accession Version	XM_006522383.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522383.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGAAG ACGCACCAGA GCGCCCTGCA GGTACAGCAG 
AAGGCAGAGG CACTGCTCCA GGCCGGCCAC TACGACGCAG ACGCCATCCG GGAATGTGCT 
GAGAAGGTGG CCCTCCACTG GCAACAGCTC ATGCTAAAGA TGGAGGACCG GCTGAAGCTT 
GTCAACGCCT CTGTGGCCTT TTACAAGACC TCTGAGCAGG TGTGCAGTGT TCTGGAGAGC 
TTGGAGCAGG AGTACCGGAG AGACGAGGAC TGGTGTGGTG GGAGAGACAA GCTGGGGCCG 
GCGGCAGAGA TGGACCACGT TATTCCACTC CTCAGCAAGC ACCTGGAGCA GAAGGAGGCC 
TTCCTGAAGG CCTGCACGCT GGCAAGGCGG AACGCCGAGG TGTTCCTCAA ATACATCCAC 
AGGAACAATG TGAGCATGCC TAGTGTTGCC AGCCACACCC GGGGGCCTGA ACAGCAAGTA 
AAAGCCATCT TGAGCGAGCT CTTGCAGAGG GAGAATCGAG TCCTGCACTT CTGGACCTTG 
AAGAAGCGGC GGTTAGACCA GTGTCAGCAA TATGTGGTGT TTGAGCGCAG TGCTAAGCAG 
GCACTGGACT GGATCCAAGA AACAGGAGAA TATTACCTCT CAACACATAC CTCCACCGGA 
GAGACCACGG AGGAGACTCA GGAGCTGCTG AAAGAGTATG GAGAATTCAG GGTGCCTGCC 
AAGCAAACGA AGGAGAAGGT GAAACTTCTG ATCCAGCTAG CAGACAGCTT TGTGGAAAAG 
GGCCACATCC ACGCCACAGA GATACGGAAA TGGGTGACCA CGGTGGACAA GCACTACCGC 
GATTTCTCCC TGAGGATGGG GAAGTACCGG TACTCGCTGG AGAAGGCGCT GGGGGTCAAC 
ACGGAGGATA ACAAGGACCT AGAGCTGGAC ATCATCCCAG CAAGCCTTTC GGATCGGGAG 
GTCAAGCTCC GGGACGCTAA CCATGAGATC AATGAAGAGA AACGGAAGTC AGCTCGAAAG 
AAAGAGTTCA TTATGGCTGA ACTGCTCCAA ACTGAGAAGG CTTATGTAAG GGACTTGCAC 
GAGTGCTTGG AGACCTACCT GTGGGAGATG ACTAGTGGTG TGGAAGAGAT ACCCCCCGGC 
ATCCTCAATA AGGAACATAT CATCTTCGGC AACATCCAGG AGATCTATGA CTTCCATAAC 
AACATCTTCC TCAAAGAGCT GGAGAAGTAC GAGCAGCTGC CTGAGGATGT GGGACACTGC 
TTTGTTACCT GGGCAGACAA ATTTCAGATG TATGTCACCT ACTGTAAAAA CAAGCCTGAT 
TCCAACCAGC TGATCCTGGA GCATGCGGGC ACCTTCTTTG ATGAGATCCA GCAGCGGCAT 
GGCCTGGCCA ACTCCATCTC CTCCTACCTA ATTAAGCCTG TCCAGAGGGT TACCAAGTAC 
CAGCTGCTCC TGAAGGAACT TCTAACTTGC TGTGAAGAAG GGAAAGGAGA GCTGAAGGAT 
GGCCTGGAGG TGATGCTCAG TGTCCCCAAG AAAGCCAACG ATGCCATGCA TGTCAGCATG 
CTGGAAGGGT TCGATGAAAA CTTGGATGTA CAGGGGGAGT TGATTCTCCA GGATGCCTTT 
CAAGTGTGGG ACCCAAAGTC GCTGATCCGG AAGGGGCGGG AGCGCCATCT GTTCCTCTTT 
GAGATCTCCT TGGTGTTTAG CAAAGAGATC AAAGACTCTT CAGGACACAC GAAATATGTT 
TACAAGAACA AGCTACTGAC CTCAGAGCTG GGTGTGACCG AGCATGTAGA AGGTGACCCC 
TGCAAATTCG CCTTGTGGTC TGGGCGCACC CCATCCTCAG ACAATAAAAC AGTGCTCAAA 
GCTTCCAACA TCGAAACCAA GCAGGAATGG ATCAAGAACA TTCGAGAAGT GATTCAGGAG 
AGGATCATTC ATCTTAAAGG CGCTTTAAAG GAGCCAATTC AGCTCCCCAA AACACCAGCC 
AAACTGAGGA ACAATAGTAA GAGGGATGGG GTAGAGGATG GTGACAGCCA AGGGGATGGG 
AGCAGCCAGC CAGATACCAT ATCCATTGCC TCCAGGACCT CTCAGAACAC AGTGGAAAGT 
GACAAGGATG GCAACCTTGT TCCTCGTTGG CACCTGGGAC CTGGAGATCC TTTCTCCACT 
TACGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522446.1
CDS	24875..29863
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKDGNLVPRWHLGPGDPFSTYV

Target ORF information:

RefSeq Version	XM_006522383.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522383.4

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGAAG ACGCACCAGA GCGCCCTGCA GGTACAGCAG 
AAGGCAGAGG CACTGCTCCA GGCCGGCCAC TACGACGCAG ACGCCATCCG GGAATGTGCT 
GAGAAGGTGG CCCTCCACTG GCAACAGCTC ATGCTAAAGA TGGAGGACCG GCTGAAGCTT 
GTCAACGCCT CTGTGGCCTT TTACAAGACC TCTGAGCAGG TGTGCAGTGT TCTGGAGAGC 
TTGGAGCAGG AGTACCGGAG AGACGAGGAC TGGTGTGGTG GGAGAGACAA GCTGGGGCCG 
GCGGCAGAGA TGGACCACGT TATTCCACTC CTCAGCAAGC ACCTGGAGCA GAAGGAGGCC 
TTCCTGAAGG CCTGCACGCT GGCAAGGCGG AACGCCGAGG TGTTCCTCAA ATACATCCAC 
AGGAACAATG TGAGCATGCC TAGTGTTGCC AGCCACACCC GGGGGCCTGA ACAGCAAGTA 
AAAGCCATCT TGAGCGAGCT CTTGCAGAGG GAGAATCGAG TCCTGCACTT CTGGACCTTG 
AAGAAGCGGC GGTTAGACCA GTGTCAGCAA TATGTGGTGT TTGAGCGCAG TGCTAAGCAG 
GCACTGGACT GGATCCAAGA AACAGGAGAA TATTACCTCT CAACACATAC CTCCACCGGA 
GAGACCACGG AGGAGACTCA GGAGCTGCTG AAAGAGTATG GAGAATTCAG GGTGCCTGCC 
AAGCAAACGA AGGAGAAGGT GAAACTTCTG ATCCAGCTAG CAGACAGCTT TGTGGAAAAG 
GGCCACATCC ACGCCACAGA GATACGGAAA TGGGTGACCA CGGTGGACAA GCACTACCGC 
GATTTCTCCC TGAGGATGGG GAAGTACCGG TACTCGCTGG AGAAGGCGCT GGGGGTCAAC 
ACGGAGGATA ACAAGGACCT AGAGCTGGAC ATCATCCCAG CAAGCCTTTC GGATCGGGAG 
GTCAAGCTCC GGGACGCTAA CCATGAGATC AATGAAGAGA AACGGAAGTC AGCTCGAAAG 
AAAGAGTTCA TTATGGCTGA ACTGCTCCAA ACTGAGAAGG CTTATGTAAG GGACTTGCAC 
GAGTGCTTGG AGACCTACCT GTGGGAGATG ACTAGTGGTG TGGAAGAGAT ACCCCCCGGC 
ATCCTCAATA AGGAACATAT CATCTTCGGC AACATCCAGG AGATCTATGA CTTCCATAAC 
AACATCTTCC TCAAAGAGCT GGAGAAGTAC GAGCAGCTGC CTGAGGATGT GGGACACTGC 
TTTGTTACCT GGGCAGACAA ATTTCAGATG TATGTCACCT ACTGTAAAAA CAAGCCTGAT 
TCCAACCAGC TGATCCTGGA GCATGCGGGC ACCTTCTTTG ATGAGATCCA GCAGCGGCAT 
GGCCTGGCCA ACTCCATCTC CTCCTACCTA ATTAAGCCTG TCCAGAGGGT TACCAAGTAC 
CAGCTGCTCC TGAAGGAACT TCTAACTTGC TGTGAAGAAG GGAAAGGAGA GCTGAAGGAT 
GGCCTGGAGG TGATGCTCAG TGTCCCCAAG AAAGCCAACG ATGCCATGCA TGTCAGCATG 
CTGGAAGGGT TCGATGAAAA CTTGGATGTA CAGGGGGAGT TGATTCTCCA GGATGCCTTT 
CAAGTGTGGG ACCCAAAGTC GCTGATCCGG AAGGGGCGGG AGCGCCATCT GTTCCTCTTT 
GAGATCTCCT TGGTGTTTAG CAAAGAGATC AAAGACTCTT CAGGACACAC GAAATATGTT 
TACAAGAACA AGCTACTGAC CTCAGAGCTG GGTGTGACCG AGCATGTAGA AGGTGACCCC 
TGCAAATTCG CCTTGTGGTC TGGGCGCACC CCATCCTCAG ACAATAAAAC AGTGCTCAAA 
GCTTCCAACA TCGAAACCAA GCAGGAATGG ATCAAGAACA TTCGAGAAGT GATTCAGGAG 
AGGATCATTC ATCTTAAAGG CGCTTTAAAG GAGCCAATTC AGCTCCCCAA AACACCAGCC 
AAACTGAGGA ACAATAGTAA GAGGGATGGG GTAGAGGATG GTGACAGCCA AGGGGATGGG 
AGCAGCCAGC CAGATACCAT ATCCATTGCC TCCAGGACCT CTCAGAACAC AGTGGAAAGT 
GACAAGGATG GCAACCTTGT TCCTCGTTGG CACCTGGGAC CTGGAGATCC TTTCTCCACT 
TACGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245968.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244270.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X30, mRNA.

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CloneID	OMu75323
Clone ID Related Accession (Same CDS sequence)	XM_011245968.2 , XM_011245968.3
Accession Version	XM_011245968.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245968.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGGATGGCA ACCTTGTTCC TCGTTGGCAC 
CTGGGACCTG GAGATCCTTT CTCCACTTAC GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244270.1
CDS	24875..29890
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKDGNLVPRWHLGPGDPFSTYV

Target ORF information:

RefSeq Version	XM_011245968.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245968.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGGATGGCA ACCTTGTTCC TCGTTGGCAC 
CTGGGACCTG GAGATCCTTT CTCCACTTAC GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245970.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244272.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X36, mRNA.

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CloneID	OMu75325
Clone ID Related Accession (Same CDS sequence)	XM_011245970.2 , XM_011245970.3
Accession Version	XM_011245970.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245970.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244272.1
CDS	24875..29485
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLP

Target ORF information:

RefSeq Version	XM_011245970.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245970.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317075.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172564.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X30, mRNA.

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CloneID	OMu79373
Clone ID Related Accession (Same CDS sequence)	XM_017317075.1 , XM_017317075.2
Accession Version	XM_017317075.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGTC CCTCTTTCAT 
GCCACTTCCT TGCAGAAGAC GCACCAGAGC GCCCTGCAGG TACAGCAGAA GGCAGAGGCA 
CTGCTCCAGG CCGGCCACTA CGACGCAGAC GCCATCCGGG AATGTGCTGA GAAGGTGGCC 
CTCCACTGGC AACAGCTCAT GCTAAAGATG GAGGACCGGC TGAAGCTTGT CAACGCCTCT 
GTGGCCTTTT ACAAGACCTC TGAGCAGGTG TGCAGTGTTC TGGAGAGCTT GGAGCAGGAG 
TACCGGAGAG ACGAGGACTG GTGTGGTGGG AGAGACAAGC TGGGGCCGGC GGCAGAGATG 
GACCACGTTA TTCCACTCCT CAGCAAGCAC CTGGAGCAGA AGGAGGCCTT CCTGAAGGCC 
TGCACGCTGG CAAGGCGGAA CGCCGAGGTG TTCCTCAAAT ACATCCACAG GAACAATGTG 
AGCATGCCTA GTGTTGCCAG CCACACCCGG GGGCCTGAAC AGCAAGTAAA AGCCATCTTG 
AGCGAGCTCT TGCAGAGGGA GAATCGAGTC CTGCACTTCT GGACCTTGAA GAAGCGGCGG 
TTAGACCAGT GTCAGCAATA TGTGGTGTTT GAGCGCAGTG CTAAGCAGGC ACTGGACTGG 
ATCCAAGAAA CAGGAGAATA TTACCTCTCA ACACATACCT CCACCGGAGA GACCACGGAG 
GAGACTCAGG AGCTGCTGAA AGAGTATGGA GAATTCAGGG TGCCTGCCAA GCAAACGAAG 
GAGAAGGTGA AACTTCTGAT CCAGCTAGCA GACAGCTTTG TGGAAAAGGG CCACATCCAC 
GCCACAGAGA TACGGAAATG GGTGACCACG GTGGACAAGC ACTACCGCGA TTTCTCCCTG 
AGGATGGGGA AGTACCGGTA CTCGCTGGAG AAGGCGCTGG GGGTCAACAC GGAGGATAAC 
AAGGACCTAG AGCTGGACAT CATCCCAGCA AGCCTTTCGG ATCGGGAGGT CAAGCTCCGG 
GACGCTAACC ATGAGATCAA TGAAGAGAAA CGGAAGTCAG CTCGAAAGAA AGAGTTCATT 
ATGGCTGAAC TGCTCCAAAC TGAGAAGGCT TATGTAAGGG ACTTGCACGA GTGCTTGGAG 
ACCTACCTGT GGGAGATGAC TAGTGGTGTG GAAGAGATAC CCCCCGGCAT CCTCAATAAG 
GAACATATCA TCTTCGGCAA CATCCAGGAG ATCTATGACT TCCATAACAA CATCTTCCTC 
AAAGAGCTGG AGAAGTACGA GCAGCTGCCT GAGGATGTGG GACACTGCTT TGTTACCTGG 
GCAGACAAAT TTCAGATGTA TGTCACCTAC TGTAAAAACA AGCCTGATTC CAACCAGCTG 
ATCCTGGAGC ATGCGGGCAC CTTCTTTGAT GAGATCCAGC AGCGGCATGG CCTGGCCAAC 
TCCATCTCCT CCTACCTAAT TAAGCCTGTC CAGAGGGTTA CCAAGTACCA GCTGCTCCTG 
AAGGAACTTC TAACTTGCTG TGAAGAAGGG AAAGGAGAGC TGAAGGATGG CCTGGAGGTG 
ATGCTCAGTG TCCCCAAGAA AGCCAACGAT GCCATGCATG TCAGCATGCT GGAAGGGTTC 
GATGAAAACT TGGATGTACA GGGGGAGTTG ATTCTCCAGG ATGCCTTTCA AGTGTGGGAC 
CCAAAGTCGC TGATCCGGAA GGGGCGGGAG CGCCATCTGT TCCTCTTTGA GATCTCCTTG 
GTGTTTAGCA AAGAGATCAA AGACTCTTCA GGACACACGA AATATGTTTA CAAGAACAAG 
CTACTGACCT CAGAGCTGGG TGTGACCGAG CATGTAGAAG GTGACCCCTG CAAATTCGCC 
TTGTGGTCTG GGCGCACCCC ATCCTCAGAC AATAAAACAG TGCTCAAAGC TTCCAACATC 
GAAACCAAGC AGGAATGGAT CAAGAACATT CGAGAAGTGA TTCAGGAGAG GATCATTCAT 
CTTAAAGGCG CTTTAAAGGA GCCAATTCAG CTCCCCAAAA CACCAGCCAA ACTGAGGAAC 
AATAGTAAGA GGGATGGGGT AGAGGATGGT GACAGCCAAG GGGATGGGAG CAGCCAGCCA 
GATACCATAT CCATTGCCTC CAGGACCTCT CAGAACACAG TGGAAAGTGA CAAGGATGGC 
AACCTTGTTC CTCGTTGGCA CCTGGGACCT GGAGATCCTT TCTCCACTTA CGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172564.1
CDS	4891..9867
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKDGNLVPRWHLGPGDPFSTYV

Target ORF information:

RefSeq Version	XM_017317075.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317075.1

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGTC CCTCTTTCAT 
GCCACTTCCT TGCAGAAGAC GCACCAGAGC GCCCTGCAGG TACAGCAGAA GGCAGAGGCA 
CTGCTCCAGG CCGGCCACTA CGACGCAGAC GCCATCCGGG AATGTGCTGA GAAGGTGGCC 
CTCCACTGGC AACAGCTCAT GCTAAAGATG GAGGACCGGC TGAAGCTTGT CAACGCCTCT 
GTGGCCTTTT ACAAGACCTC TGAGCAGGTG TGCAGTGTTC TGGAGAGCTT GGAGCAGGAG 
TACCGGAGAG ACGAGGACTG GTGTGGTGGG AGAGACAAGC TGGGGCCGGC GGCAGAGATG 
GACCACGTTA TTCCACTCCT CAGCAAGCAC CTGGAGCAGA AGGAGGCCTT CCTGAAGGCC 
TGCACGCTGG CAAGGCGGAA CGCCGAGGTG TTCCTCAAAT ACATCCACAG GAACAATGTG 
AGCATGCCTA GTGTTGCCAG CCACACCCGG GGGCCTGAAC AGCAAGTAAA AGCCATCTTG 
AGCGAGCTCT TGCAGAGGGA GAATCGAGTC CTGCACTTCT GGACCTTGAA GAAGCGGCGG 
TTAGACCAGT GTCAGCAATA TGTGGTGTTT GAGCGCAGTG CTAAGCAGGC ACTGGACTGG 
ATCCAAGAAA CAGGAGAATA TTACCTCTCA ACACATACCT CCACCGGAGA GACCACGGAG 
GAGACTCAGG AGCTGCTGAA AGAGTATGGA GAATTCAGGG TGCCTGCCAA GCAAACGAAG 
GAGAAGGTGA AACTTCTGAT CCAGCTAGCA GACAGCTTTG TGGAAAAGGG CCACATCCAC 
GCCACAGAGA TACGGAAATG GGTGACCACG GTGGACAAGC ACTACCGCGA TTTCTCCCTG 
AGGATGGGGA AGTACCGGTA CTCGCTGGAG AAGGCGCTGG GGGTCAACAC GGAGGATAAC 
AAGGACCTAG AGCTGGACAT CATCCCAGCA AGCCTTTCGG ATCGGGAGGT CAAGCTCCGG 
GACGCTAACC ATGAGATCAA TGAAGAGAAA CGGAAGTCAG CTCGAAAGAA AGAGTTCATT 
ATGGCTGAAC TGCTCCAAAC TGAGAAGGCT TATGTAAGGG ACTTGCACGA GTGCTTGGAG 
ACCTACCTGT GGGAGATGAC TAGTGGTGTG GAAGAGATAC CCCCCGGCAT CCTCAATAAG 
GAACATATCA TCTTCGGCAA CATCCAGGAG ATCTATGACT TCCATAACAA CATCTTCCTC 
AAAGAGCTGG AGAAGTACGA GCAGCTGCCT GAGGATGTGG GACACTGCTT TGTTACCTGG 
GCAGACAAAT TTCAGATGTA TGTCACCTAC TGTAAAAACA AGCCTGATTC CAACCAGCTG 
ATCCTGGAGC ATGCGGGCAC CTTCTTTGAT GAGATCCAGC AGCGGCATGG CCTGGCCAAC 
TCCATCTCCT CCTACCTAAT TAAGCCTGTC CAGAGGGTTA CCAAGTACCA GCTGCTCCTG 
AAGGAACTTC TAACTTGCTG TGAAGAAGGG AAAGGAGAGC TGAAGGATGG CCTGGAGGTG 
ATGCTCAGTG TCCCCAAGAA AGCCAACGAT GCCATGCATG TCAGCATGCT GGAAGGGTTC 
GATGAAAACT TGGATGTACA GGGGGAGTTG ATTCTCCAGG ATGCCTTTCA AGTGTGGGAC 
CCAAAGTCGC TGATCCGGAA GGGGCGGGAG CGCCATCTGT TCCTCTTTGA GATCTCCTTG 
GTGTTTAGCA AAGAGATCAA AGACTCTTCA GGACACACGA AATATGTTTA CAAGAACAAG 
CTACTGACCT CAGAGCTGGG TGTGACCGAG CATGTAGAAG GTGACCCCTG CAAATTCGCC 
TTGTGGTCTG GGCGCACCCC ATCCTCAGAC AATAAAACAG TGCTCAAAGC TTCCAACATC 
GAAACCAAGC AGGAATGGAT CAAGAACATT CGAGAAGTGA TTCAGGAGAG GATCATTCAT 
CTTAAAGGCG CTTTAAAGGA GCCAATTCAG CTCCCCAAAA CACCAGCCAA ACTGAGGAAC 
AATAGTAAGA GGGATGGGGT AGAGGATGGT GACAGCCAAG GGGATGGGAG CAGCCAGCCA 
GATACCATAT CCATTGCCTC CAGGACCTCT CAGAACACAG TGGAAAGTGA CAAGGATGGC 
AACCTTGTTC CTCGTTGGCA CCTGGGACCT GGAGATCCTT TCTCCACTTA CGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317075.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172564.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OMu79373
Clone ID Related Accession (Same CDS sequence)	XM_017317075.1 , XM_017317075.2
Accession Version	XM_017317075.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317075.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGTC CCTCTTTCAT 
GCCACTTCCT TGCAGAAGAC GCACCAGAGC GCCCTGCAGG TACAGCAGAA GGCAGAGGCA 
CTGCTCCAGG CCGGCCACTA CGACGCAGAC GCCATCCGGG AATGTGCTGA GAAGGTGGCC 
CTCCACTGGC AACAGCTCAT GCTAAAGATG GAGGACCGGC TGAAGCTTGT CAACGCCTCT 
GTGGCCTTTT ACAAGACCTC TGAGCAGGTG TGCAGTGTTC TGGAGAGCTT GGAGCAGGAG 
TACCGGAGAG ACGAGGACTG GTGTGGTGGG AGAGACAAGC TGGGGCCGGC GGCAGAGATG 
GACCACGTTA TTCCACTCCT CAGCAAGCAC CTGGAGCAGA AGGAGGCCTT CCTGAAGGCC 
TGCACGCTGG CAAGGCGGAA CGCCGAGGTG TTCCTCAAAT ACATCCACAG GAACAATGTG 
AGCATGCCTA GTGTTGCCAG CCACACCCGG GGGCCTGAAC AGCAAGTAAA AGCCATCTTG 
AGCGAGCTCT TGCAGAGGGA GAATCGAGTC CTGCACTTCT GGACCTTGAA GAAGCGGCGG 
TTAGACCAGT GTCAGCAATA TGTGGTGTTT GAGCGCAGTG CTAAGCAGGC ACTGGACTGG 
ATCCAAGAAA CAGGAGAATA TTACCTCTCA ACACATACCT CCACCGGAGA GACCACGGAG 
GAGACTCAGG AGCTGCTGAA AGAGTATGGA GAATTCAGGG TGCCTGCCAA GCAAACGAAG 
GAGAAGGTGA AACTTCTGAT CCAGCTAGCA GACAGCTTTG TGGAAAAGGG CCACATCCAC 
GCCACAGAGA TACGGAAATG GGTGACCACG GTGGACAAGC ACTACCGCGA TTTCTCCCTG 
AGGATGGGGA AGTACCGGTA CTCGCTGGAG AAGGCGCTGG GGGTCAACAC GGAGGATAAC 
AAGGACCTAG AGCTGGACAT CATCCCAGCA AGCCTTTCGG ATCGGGAGGT CAAGCTCCGG 
GACGCTAACC ATGAGATCAA TGAAGAGAAA CGGAAGTCAG CTCGAAAGAA AGAGTTCATT 
ATGGCTGAAC TGCTCCAAAC TGAGAAGGCT TATGTAAGGG ACTTGCACGA GTGCTTGGAG 
ACCTACCTGT GGGAGATGAC TAGTGGTGTG GAAGAGATAC CCCCCGGCAT CCTCAATAAG 
GAACATATCA TCTTCGGCAA CATCCAGGAG ATCTATGACT TCCATAACAA CATCTTCCTC 
AAAGAGCTGG AGAAGTACGA GCAGCTGCCT GAGGATGTGG GACACTGCTT TGTTACCTGG 
GCAGACAAAT TTCAGATGTA TGTCACCTAC TGTAAAAACA AGCCTGATTC CAACCAGCTG 
ATCCTGGAGC ATGCGGGCAC CTTCTTTGAT GAGATCCAGC AGCGGCATGG CCTGGCCAAC 
TCCATCTCCT CCTACCTAAT TAAGCCTGTC CAGAGGGTTA CCAAGTACCA GCTGCTCCTG 
AAGGAACTTC TAACTTGCTG TGAAGAAGGG AAAGGAGAGC TGAAGGATGG CCTGGAGGTG 
ATGCTCAGTG TCCCCAAGAA AGCCAACGAT GCCATGCATG TCAGCATGCT GGAAGGGTTC 
GATGAAAACT TGGATGTACA GGGGGAGTTG ATTCTCCAGG ATGCCTTTCA AGTGTGGGAC 
CCAAAGTCGC TGATCCGGAA GGGGCGGGAG CGCCATCTGT TCCTCTTTGA GATCTCCTTG 
GTGTTTAGCA AAGAGATCAA AGACTCTTCA GGACACACGA AATATGTTTA CAAGAACAAG 
CTACTGACCT CAGAGCTGGG TGTGACCGAG CATGTAGAAG GTGACCCCTG CAAATTCGCC 
TTGTGGTCTG GGCGCACCCC ATCCTCAGAC AATAAAACAG TGCTCAAAGC TTCCAACATC 
GAAACCAAGC AGGAATGGAT CAAGAACATT CGAGAAGTGA TTCAGGAGAG GATCATTCAT 
CTTAAAGGCG CTTTAAAGGA GCCAATTCAG CTCCCCAAAA CACCAGCCAA ACTGAGGAAC 
AATAGTAAGA GGGATGGGGT AGAGGATGGT GACAGCCAAG GGGATGGGAG CAGCCAGCCA 
GATACCATAT CCATTGCCTC CAGGACCTCT CAGAACACAG TGGAAAGTGA CAAGGATGGC 
AACCTTGTTC CTCGTTGGCA CCTGGGACCT GGAGATCCTT TCTCCACTTA CGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172564.1
CDS	24875..29851
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKDGNLVPRWHLGPGDPFSTYV

Target ORF information:

RefSeq Version	XM_017317075.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317075.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGTC CCTCTTTCAT 
GCCACTTCCT TGCAGAAGAC GCACCAGAGC GCCCTGCAGG TACAGCAGAA GGCAGAGGCA 
CTGCTCCAGG CCGGCCACTA CGACGCAGAC GCCATCCGGG AATGTGCTGA GAAGGTGGCC 
CTCCACTGGC AACAGCTCAT GCTAAAGATG GAGGACCGGC TGAAGCTTGT CAACGCCTCT 
GTGGCCTTTT ACAAGACCTC TGAGCAGGTG TGCAGTGTTC TGGAGAGCTT GGAGCAGGAG 
TACCGGAGAG ACGAGGACTG GTGTGGTGGG AGAGACAAGC TGGGGCCGGC GGCAGAGATG 
GACCACGTTA TTCCACTCCT CAGCAAGCAC CTGGAGCAGA AGGAGGCCTT CCTGAAGGCC 
TGCACGCTGG CAAGGCGGAA CGCCGAGGTG TTCCTCAAAT ACATCCACAG GAACAATGTG 
AGCATGCCTA GTGTTGCCAG CCACACCCGG GGGCCTGAAC AGCAAGTAAA AGCCATCTTG 
AGCGAGCTCT TGCAGAGGGA GAATCGAGTC CTGCACTTCT GGACCTTGAA GAAGCGGCGG 
TTAGACCAGT GTCAGCAATA TGTGGTGTTT GAGCGCAGTG CTAAGCAGGC ACTGGACTGG 
ATCCAAGAAA CAGGAGAATA TTACCTCTCA ACACATACCT CCACCGGAGA GACCACGGAG 
GAGACTCAGG AGCTGCTGAA AGAGTATGGA GAATTCAGGG TGCCTGCCAA GCAAACGAAG 
GAGAAGGTGA AACTTCTGAT CCAGCTAGCA GACAGCTTTG TGGAAAAGGG CCACATCCAC 
GCCACAGAGA TACGGAAATG GGTGACCACG GTGGACAAGC ACTACCGCGA TTTCTCCCTG 
AGGATGGGGA AGTACCGGTA CTCGCTGGAG AAGGCGCTGG GGGTCAACAC GGAGGATAAC 
AAGGACCTAG AGCTGGACAT CATCCCAGCA AGCCTTTCGG ATCGGGAGGT CAAGCTCCGG 
GACGCTAACC ATGAGATCAA TGAAGAGAAA CGGAAGTCAG CTCGAAAGAA AGAGTTCATT 
ATGGCTGAAC TGCTCCAAAC TGAGAAGGCT TATGTAAGGG ACTTGCACGA GTGCTTGGAG 
ACCTACCTGT GGGAGATGAC TAGTGGTGTG GAAGAGATAC CCCCCGGCAT CCTCAATAAG 
GAACATATCA TCTTCGGCAA CATCCAGGAG ATCTATGACT TCCATAACAA CATCTTCCTC 
AAAGAGCTGG AGAAGTACGA GCAGCTGCCT GAGGATGTGG GACACTGCTT TGTTACCTGG 
GCAGACAAAT TTCAGATGTA TGTCACCTAC TGTAAAAACA AGCCTGATTC CAACCAGCTG 
ATCCTGGAGC ATGCGGGCAC CTTCTTTGAT GAGATCCAGC AGCGGCATGG CCTGGCCAAC 
TCCATCTCCT CCTACCTAAT TAAGCCTGTC CAGAGGGTTA CCAAGTACCA GCTGCTCCTG 
AAGGAACTTC TAACTTGCTG TGAAGAAGGG AAAGGAGAGC TGAAGGATGG CCTGGAGGTG 
ATGCTCAGTG TCCCCAAGAA AGCCAACGAT GCCATGCATG TCAGCATGCT GGAAGGGTTC 
GATGAAAACT TGGATGTACA GGGGGAGTTG ATTCTCCAGG ATGCCTTTCA AGTGTGGGAC 
CCAAAGTCGC TGATCCGGAA GGGGCGGGAG CGCCATCTGT TCCTCTTTGA GATCTCCTTG 
GTGTTTAGCA AAGAGATCAA AGACTCTTCA GGACACACGA AATATGTTTA CAAGAACAAG 
CTACTGACCT CAGAGCTGGG TGTGACCGAG CATGTAGAAG GTGACCCCTG CAAATTCGCC 
TTGTGGTCTG GGCGCACCCC ATCCTCAGAC AATAAAACAG TGCTCAAAGC TTCCAACATC 
GAAACCAAGC AGGAATGGAT CAAGAACATT CGAGAAGTGA TTCAGGAGAG GATCATTCAT 
CTTAAAGGCG CTTTAAAGGA GCCAATTCAG CTCCCCAAAA CACCAGCCAA ACTGAGGAAC 
AATAGTAAGA GGGATGGGGT AGAGGATGGT GACAGCCAAG GGGATGGGAG CAGCCAGCCA 
GATACCATAT CCATTGCCTC CAGGACCTCT CAGAACACAG TGGAAAGTGA CAAGGATGGC 
AACCTTGTTC CTCGTTGGCA CCTGGGACCT GGAGATCCTT TCTCCACTTA CGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317076.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172565.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X34, mRNA.

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CloneID	OMu79374
Clone ID Related Accession (Same CDS sequence)	XM_017317076.2 , XM_017317076.1
Accession Version	XM_017317076.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317076.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGAA GACGCACCAG 
AGCGCCCTGC AGGTACAGCA GAAGGCAGAG GCACTGCTCC AGGCCGGCCA CTACGACGCA 
GACGCCATCC GGGAATGTGC TGAGAAGGTG GCCCTCCACT GGCAACAGCT CATGCTAAAG 
ATGGAGGACC GGCTGAAGCT TGTCAACGCC TCTGTGGCCT TTTACAAGAC CTCTGAGCAG 
GTGTGCAGTG TTCTGGAGAG CTTGGAGCAG GAGTACCGGA GAGACGAGGA CTGGTGTGGT 
GGGAGAGACA AGCTGGGGCC GGCGGCAGAG ATGGACCACG TTATTCCACT CCTCAGCAAG 
CACCTGGAGC AGAAGGAGGC CTTCCTGAAG GCCTGCACGC TGGCAAGGCG GAACGCCGAG 
GTGTTCCTCA AATACATCCA CAGGAACAAT GTGAGCATGC CTAGTGTTGC CAGCCACACC 
CGGGGGCCTG AACAGCAAGT AAAAGCCATC TTGAGCGAGC TCTTGCAGAG GGAGAATCGA 
GTCCTGCACT TCTGGACCTT GAAGAAGCGG CGGTTAGACC AGTGTCAGCA ATATGTGGTG 
TTTGAGCGCA GTGCTAAGCA GGCACTGGAC TGGATCCAAG AAACAGGAGA ATATTACCTC 
TCAACACATA CCTCCACCGG AGAGACCACG GAGGAGACTC AGGAGCTGCT GAAAGAGTAT 
GGAGAATTCA GGGTGCCTGC CAAGCAAACG AAGGAGAAGG TGAAACTTCT GATCCAGCTA 
GCAGACAGCT TTGTGGAAAA GGGCCACATC CACGCCACAG AGATACGGAA ATGGGTGACC 
ACGGTGGACA AGCACTACCG CGATTTCTCC CTGAGGATGG GGAAGTACCG GTACTCGCTG 
GAGAAGGCGC TGGGGGTCAA CACGGAGGAT AACAAGGACC TAGAGCTGGA CATCATCCCA 
GCAAGCCTTT CGGATCGGGA GGTCAAGCTC CGGGACGCTA ACCATGAGAT CAATGAAGAG 
AAACGGAAGT CAGCTCGAAA GAAAGAGTTC ATTATGGCTG AACTGCTCCA AACTGAGAAG 
GCTTATGTAA GGGACTTGCA CGAGTGCTTG GAGACCTACC TGTGGGAGAT GACTAGTGGT 
GTGGAAGAGA TACCCCCCGG CATCCTCAAT AAGGAACATA TCATCTTCGG CAACATCCAG 
GAGATCTATG ACTTCCATAA CAACATCTTC CTCAAAGAGC TGGAGAAGTA CGAGCAGCTG 
CCTGAGGATG TGGGACACTG CTTTGTTACC TGGGCAGACA AATTTCAGAT GTATGTCACC 
TACTGTAAAA ACAAGCCTGA TTCCAACCAG CTGATCCTGG AGCATGCGGG CACCTTCTTT 
GATGAGATCC AGCAGCGGCA TGGCCTGGCC AACTCCATCT CCTCCTACCT AATTAAGCCT 
GTCCAGAGGG TTACCAAGTA CCAGCTGCTC CTGAAGGAAC TTCTAACTTG CTGTGAAGAA 
GGGAAAGGAG AGCTGAAGGA TGGCCTGGAG GTGATGCTCA GTGTCCCCAA GAAAGCCAAC 
GATGCCATGC ATGTCAGCAT GCTGGAAGGG TTCGATGAAA ACTTGGATGT ACAGGGGGAG 
TTGATTCTCC AGGATGCCTT TCAAGTGTGG GACCCAAAGT CGCTGATCCG GAAGGGGCGG 
GAGCGCCATC TGTTCCTCTT TGAGATCTCC TTGGTGTTTA GCAAAGAGAT CAAAGACTCT 
TCAGGACACA CGAAATATGT TTACAAGAAC AAGCTACTGA CCTCAGAGCT GGGTGTGACC 
GAGCATGTAG AAGGTGACCC CTGCAAATTC GCCTTGTGGT CTGGGCGCAC CCCATCCTCA 
GACAATAAAA CAGTGCTCAA AGCTTCCAAC ATCGAAACCA AGCAGGAATG GATCAAGAAC 
ATTCGAGAAG TGATTCAGGA GAGGATCATT CATCTTAAAG GCGCTTTAAA GGAGCCAATT 
CAGCTCCCCA AAACACCAGC CAAACTGAGG AACAATAGTA AGAGGGATGG GGTAGAGGAT 
GGTGACAGCC AAGGGGATGG GAGCAGCCAG CCAGATACCA TATCCATTGC CTCCAGGACC 
TCTCAGAACA CAGTGGAAAG TGACAAGGAT GGCAACCTTG TTCCTCGTTG GCACCTGGGA 
CCTGGAGATC CTTTCTCCAC TTACGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172565.1
CDS	24875..29824
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKDGNLVPRWHLGPGDPFSTYV

Target ORF information:

RefSeq Version	XM_017317076.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317076.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGAA GACGCACCAG 
AGCGCCCTGC AGGTACAGCA GAAGGCAGAG GCACTGCTCC AGGCCGGCCA CTACGACGCA 
GACGCCATCC GGGAATGTGC TGAGAAGGTG GCCCTCCACT GGCAACAGCT CATGCTAAAG 
ATGGAGGACC GGCTGAAGCT TGTCAACGCC TCTGTGGCCT TTTACAAGAC CTCTGAGCAG 
GTGTGCAGTG TTCTGGAGAG CTTGGAGCAG GAGTACCGGA GAGACGAGGA CTGGTGTGGT 
GGGAGAGACA AGCTGGGGCC GGCGGCAGAG ATGGACCACG TTATTCCACT CCTCAGCAAG 
CACCTGGAGC AGAAGGAGGC CTTCCTGAAG GCCTGCACGC TGGCAAGGCG GAACGCCGAG 
GTGTTCCTCA AATACATCCA CAGGAACAAT GTGAGCATGC CTAGTGTTGC CAGCCACACC 
CGGGGGCCTG AACAGCAAGT AAAAGCCATC TTGAGCGAGC TCTTGCAGAG GGAGAATCGA 
GTCCTGCACT TCTGGACCTT GAAGAAGCGG CGGTTAGACC AGTGTCAGCA ATATGTGGTG 
TTTGAGCGCA GTGCTAAGCA GGCACTGGAC TGGATCCAAG AAACAGGAGA ATATTACCTC 
TCAACACATA CCTCCACCGG AGAGACCACG GAGGAGACTC AGGAGCTGCT GAAAGAGTAT 
GGAGAATTCA GGGTGCCTGC CAAGCAAACG AAGGAGAAGG TGAAACTTCT GATCCAGCTA 
GCAGACAGCT TTGTGGAAAA GGGCCACATC CACGCCACAG AGATACGGAA ATGGGTGACC 
ACGGTGGACA AGCACTACCG CGATTTCTCC CTGAGGATGG GGAAGTACCG GTACTCGCTG 
GAGAAGGCGC TGGGGGTCAA CACGGAGGAT AACAAGGACC TAGAGCTGGA CATCATCCCA 
GCAAGCCTTT CGGATCGGGA GGTCAAGCTC CGGGACGCTA ACCATGAGAT CAATGAAGAG 
AAACGGAAGT CAGCTCGAAA GAAAGAGTTC ATTATGGCTG AACTGCTCCA AACTGAGAAG 
GCTTATGTAA GGGACTTGCA CGAGTGCTTG GAGACCTACC TGTGGGAGAT GACTAGTGGT 
GTGGAAGAGA TACCCCCCGG CATCCTCAAT AAGGAACATA TCATCTTCGG CAACATCCAG 
GAGATCTATG ACTTCCATAA CAACATCTTC CTCAAAGAGC TGGAGAAGTA CGAGCAGCTG 
CCTGAGGATG TGGGACACTG CTTTGTTACC TGGGCAGACA AATTTCAGAT GTATGTCACC 
TACTGTAAAA ACAAGCCTGA TTCCAACCAG CTGATCCTGG AGCATGCGGG CACCTTCTTT 
GATGAGATCC AGCAGCGGCA TGGCCTGGCC AACTCCATCT CCTCCTACCT AATTAAGCCT 
GTCCAGAGGG TTACCAAGTA CCAGCTGCTC CTGAAGGAAC TTCTAACTTG CTGTGAAGAA 
GGGAAAGGAG AGCTGAAGGA TGGCCTGGAG GTGATGCTCA GTGTCCCCAA GAAAGCCAAC 
GATGCCATGC ATGTCAGCAT GCTGGAAGGG TTCGATGAAA ACTTGGATGT ACAGGGGGAG 
TTGATTCTCC AGGATGCCTT TCAAGTGTGG GACCCAAAGT CGCTGATCCG GAAGGGGCGG 
GAGCGCCATC TGTTCCTCTT TGAGATCTCC TTGGTGTTTA GCAAAGAGAT CAAAGACTCT 
TCAGGACACA CGAAATATGT TTACAAGAAC AAGCTACTGA CCTCAGAGCT GGGTGTGACC 
GAGCATGTAG AAGGTGACCC CTGCAAATTC GCCTTGTGGT CTGGGCGCAC CCCATCCTCA 
GACAATAAAA CAGTGCTCAA AGCTTCCAAC ATCGAAACCA AGCAGGAATG GATCAAGAAC 
ATTCGAGAAG TGATTCAGGA GAGGATCATT CATCTTAAAG GCGCTTTAAA GGAGCCAATT 
CAGCTCCCCA AAACACCAGC CAAACTGAGG AACAATAGTA AGAGGGATGG GGTAGAGGAT 
GGTGACAGCC AAGGGGATGG GAGCAGCCAG CCAGATACCA TATCCATTGC CTCCAGGACC 
TCTCAGAACA CAGTGGAAAG TGACAAGGAT GGCAACCTTG TTCCTCGTTG GCACCTGGGA 
CCTGGAGATC CTTTCTCCAC TTACGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317076.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172565.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X31, mRNA.

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CloneID	OMu79374
Clone ID Related Accession (Same CDS sequence)	XM_017317076.2 , XM_017317076.1
Accession Version	XM_017317076.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGAA GACGCACCAG 
AGCGCCCTGC AGGTACAGCA GAAGGCAGAG GCACTGCTCC AGGCCGGCCA CTACGACGCA 
GACGCCATCC GGGAATGTGC TGAGAAGGTG GCCCTCCACT GGCAACAGCT CATGCTAAAG 
ATGGAGGACC GGCTGAAGCT TGTCAACGCC TCTGTGGCCT TTTACAAGAC CTCTGAGCAG 
GTGTGCAGTG TTCTGGAGAG CTTGGAGCAG GAGTACCGGA GAGACGAGGA CTGGTGTGGT 
GGGAGAGACA AGCTGGGGCC GGCGGCAGAG ATGGACCACG TTATTCCACT CCTCAGCAAG 
CACCTGGAGC AGAAGGAGGC CTTCCTGAAG GCCTGCACGC TGGCAAGGCG GAACGCCGAG 
GTGTTCCTCA AATACATCCA CAGGAACAAT GTGAGCATGC CTAGTGTTGC CAGCCACACC 
CGGGGGCCTG AACAGCAAGT AAAAGCCATC TTGAGCGAGC TCTTGCAGAG GGAGAATCGA 
GTCCTGCACT TCTGGACCTT GAAGAAGCGG CGGTTAGACC AGTGTCAGCA ATATGTGGTG 
TTTGAGCGCA GTGCTAAGCA GGCACTGGAC TGGATCCAAG AAACAGGAGA ATATTACCTC 
TCAACACATA CCTCCACCGG AGAGACCACG GAGGAGACTC AGGAGCTGCT GAAAGAGTAT 
GGAGAATTCA GGGTGCCTGC CAAGCAAACG AAGGAGAAGG TGAAACTTCT GATCCAGCTA 
GCAGACAGCT TTGTGGAAAA GGGCCACATC CACGCCACAG AGATACGGAA ATGGGTGACC 
ACGGTGGACA AGCACTACCG CGATTTCTCC CTGAGGATGG GGAAGTACCG GTACTCGCTG 
GAGAAGGCGC TGGGGGTCAA CACGGAGGAT AACAAGGACC TAGAGCTGGA CATCATCCCA 
GCAAGCCTTT CGGATCGGGA GGTCAAGCTC CGGGACGCTA ACCATGAGAT CAATGAAGAG 
AAACGGAAGT CAGCTCGAAA GAAAGAGTTC ATTATGGCTG AACTGCTCCA AACTGAGAAG 
GCTTATGTAA GGGACTTGCA CGAGTGCTTG GAGACCTACC TGTGGGAGAT GACTAGTGGT 
GTGGAAGAGA TACCCCCCGG CATCCTCAAT AAGGAACATA TCATCTTCGG CAACATCCAG 
GAGATCTATG ACTTCCATAA CAACATCTTC CTCAAAGAGC TGGAGAAGTA CGAGCAGCTG 
CCTGAGGATG TGGGACACTG CTTTGTTACC TGGGCAGACA AATTTCAGAT GTATGTCACC 
TACTGTAAAA ACAAGCCTGA TTCCAACCAG CTGATCCTGG AGCATGCGGG CACCTTCTTT 
GATGAGATCC AGCAGCGGCA TGGCCTGGCC AACTCCATCT CCTCCTACCT AATTAAGCCT 
GTCCAGAGGG TTACCAAGTA CCAGCTGCTC CTGAAGGAAC TTCTAACTTG CTGTGAAGAA 
GGGAAAGGAG AGCTGAAGGA TGGCCTGGAG GTGATGCTCA GTGTCCCCAA GAAAGCCAAC 
GATGCCATGC ATGTCAGCAT GCTGGAAGGG TTCGATGAAA ACTTGGATGT ACAGGGGGAG 
TTGATTCTCC AGGATGCCTT TCAAGTGTGG GACCCAAAGT CGCTGATCCG GAAGGGGCGG 
GAGCGCCATC TGTTCCTCTT TGAGATCTCC TTGGTGTTTA GCAAAGAGAT CAAAGACTCT 
TCAGGACACA CGAAATATGT TTACAAGAAC AAGCTACTGA CCTCAGAGCT GGGTGTGACC 
GAGCATGTAG AAGGTGACCC CTGCAAATTC GCCTTGTGGT CTGGGCGCAC CCCATCCTCA 
GACAATAAAA CAGTGCTCAA AGCTTCCAAC ATCGAAACCA AGCAGGAATG GATCAAGAAC 
ATTCGAGAAG TGATTCAGGA GAGGATCATT CATCTTAAAG GCGCTTTAAA GGAGCCAATT 
CAGCTCCCCA AAACACCAGC CAAACTGAGG AACAATAGTA AGAGGGATGG GGTAGAGGAT 
GGTGACAGCC AAGGGGATGG GAGCAGCCAG CCAGATACCA TATCCATTGC CTCCAGGACC 
TCTCAGAACA CAGTGGAAAG TGACAAGGAT GGCAACCTTG TTCCTCGTTG GCACCTGGGA 
CCTGGAGATC CTTTCTCCAC TTACGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172565.1
CDS	4891..9840
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKDGNLVPRWHLGPGDPFSTYV

Target ORF information:

RefSeq Version	XM_017317076.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317076.1

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGAA GACGCACCAG 
AGCGCCCTGC AGGTACAGCA GAAGGCAGAG GCACTGCTCC AGGCCGGCCA CTACGACGCA 
GACGCCATCC GGGAATGTGC TGAGAAGGTG GCCCTCCACT GGCAACAGCT CATGCTAAAG 
ATGGAGGACC GGCTGAAGCT TGTCAACGCC TCTGTGGCCT TTTACAAGAC CTCTGAGCAG 
GTGTGCAGTG TTCTGGAGAG CTTGGAGCAG GAGTACCGGA GAGACGAGGA CTGGTGTGGT 
GGGAGAGACA AGCTGGGGCC GGCGGCAGAG ATGGACCACG TTATTCCACT CCTCAGCAAG 
CACCTGGAGC AGAAGGAGGC CTTCCTGAAG GCCTGCACGC TGGCAAGGCG GAACGCCGAG 
GTGTTCCTCA AATACATCCA CAGGAACAAT GTGAGCATGC CTAGTGTTGC CAGCCACACC 
CGGGGGCCTG AACAGCAAGT AAAAGCCATC TTGAGCGAGC TCTTGCAGAG GGAGAATCGA 
GTCCTGCACT TCTGGACCTT GAAGAAGCGG CGGTTAGACC AGTGTCAGCA ATATGTGGTG 
TTTGAGCGCA GTGCTAAGCA GGCACTGGAC TGGATCCAAG AAACAGGAGA ATATTACCTC 
TCAACACATA CCTCCACCGG AGAGACCACG GAGGAGACTC AGGAGCTGCT GAAAGAGTAT 
GGAGAATTCA GGGTGCCTGC CAAGCAAACG AAGGAGAAGG TGAAACTTCT GATCCAGCTA 
GCAGACAGCT TTGTGGAAAA GGGCCACATC CACGCCACAG AGATACGGAA ATGGGTGACC 
ACGGTGGACA AGCACTACCG CGATTTCTCC CTGAGGATGG GGAAGTACCG GTACTCGCTG 
GAGAAGGCGC TGGGGGTCAA CACGGAGGAT AACAAGGACC TAGAGCTGGA CATCATCCCA 
GCAAGCCTTT CGGATCGGGA GGTCAAGCTC CGGGACGCTA ACCATGAGAT CAATGAAGAG 
AAACGGAAGT CAGCTCGAAA GAAAGAGTTC ATTATGGCTG AACTGCTCCA AACTGAGAAG 
GCTTATGTAA GGGACTTGCA CGAGTGCTTG GAGACCTACC TGTGGGAGAT GACTAGTGGT 
GTGGAAGAGA TACCCCCCGG CATCCTCAAT AAGGAACATA TCATCTTCGG CAACATCCAG 
GAGATCTATG ACTTCCATAA CAACATCTTC CTCAAAGAGC TGGAGAAGTA CGAGCAGCTG 
CCTGAGGATG TGGGACACTG CTTTGTTACC TGGGCAGACA AATTTCAGAT GTATGTCACC 
TACTGTAAAA ACAAGCCTGA TTCCAACCAG CTGATCCTGG AGCATGCGGG CACCTTCTTT 
GATGAGATCC AGCAGCGGCA TGGCCTGGCC AACTCCATCT CCTCCTACCT AATTAAGCCT 
GTCCAGAGGG TTACCAAGTA CCAGCTGCTC CTGAAGGAAC TTCTAACTTG CTGTGAAGAA 
GGGAAAGGAG AGCTGAAGGA TGGCCTGGAG GTGATGCTCA GTGTCCCCAA GAAAGCCAAC 
GATGCCATGC ATGTCAGCAT GCTGGAAGGG TTCGATGAAA ACTTGGATGT ACAGGGGGAG 
TTGATTCTCC AGGATGCCTT TCAAGTGTGG GACCCAAAGT CGCTGATCCG GAAGGGGCGG 
GAGCGCCATC TGTTCCTCTT TGAGATCTCC TTGGTGTTTA GCAAAGAGAT CAAAGACTCT 
TCAGGACACA CGAAATATGT TTACAAGAAC AAGCTACTGA CCTCAGAGCT GGGTGTGACC 
GAGCATGTAG AAGGTGACCC CTGCAAATTC GCCTTGTGGT CTGGGCGCAC CCCATCCTCA 
GACAATAAAA CAGTGCTCAA AGCTTCCAAC ATCGAAACCA AGCAGGAATG GATCAAGAAC 
ATTCGAGAAG TGATTCAGGA GAGGATCATT CATCTTAAAG GCGCTTTAAA GGAGCCAATT 
CAGCTCCCCA AAACACCAGC CAAACTGAGG AACAATAGTA AGAGGGATGG GGTAGAGGAT 
GGTGACAGCC AAGGGGATGG GAGCAGCCAG CCAGATACCA TATCCATTGC CTCCAGGACC 
TCTCAGAACA CAGTGGAAAG TGACAAGGAT GGCAACCTTG TTCCTCGTTG GCACCTGGGA 
CCTGGAGATC CTTTCTCCAC TTACGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( NM_001164268.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157740.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant 2, mRNA.

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CloneID	OMu21858
Clone ID Related Accession (Same CDS sequence)	NM_001164268.1
Accession Version	NM_001164268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	kalirin isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC157950.1 and AK147518.1. On or before Aug 20, 2009 this sequence version replaced XM_001001454.1, XM_001481028.1, XM_001481029.1, XM_894473.2, XM_001001845.1. Transcript Variant: This variant (2) uses an alternate splice pattern in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a substantially shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147314.1, AK147518.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGAT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC TATCCTAAAG 
GAGAAAGTGG CCTTTGTGTC TGGGGGGCGT GACAAGCGAG GTGGACCCAT CCTGACCTTC 
CCTGCCCGAA GCAATCATGA CAGAATAAGA CAGGAAGACC TACGGAAACT TGTGACGTAT 
TTGGCCAGCG TGCCAAGTGA GGACGTTTGC AAACGTGGCT TCACCGTCAT CATCGACATG 
CGGGGCTCCA AGTGGGATCT CATCAAGCCC CTCCTCAAGA CGCTGCAGGA AGCCTTCCCC 
GCCGAGATCC ATGTGGCCCT CATCATCAAG CCTGACAACT TCTGGCAGAA GCAGAAGACC 
AACTTTGGCA GCTCCAAATT CATCTTTGAG ACAAGCATGG TGTCTGTGGA GGGCCTCACG 
AAGCTGGTGG ACCCCTCCCA GCTGACAGAG GAGTTTGACG GCTCCCTGGA TTACAACCAT 
GAGGAGTGGA TTGAGTTGCG CCTCTCCCTG GAGGAGTTCT TCAACAGTGC GGTCCACCTG 
CTCTCACGCC TTGAGGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC TGTGGATGTG 
GAGGGCTCTC GGAGGCTTAT CGACGAGCAC ACGCAACTTA AGAAAAAGGT GCTGAAGGCC 
CCCGTGGAAG AGCTGGACCG GGAGGGGCAG CGTCTACTGC AGTGCATCCG CTGCAGCGAT 
GGCTTCTCAG GGCGCAACTG CATCCCAGGA AGTGCCGACT TCCAGAGCCT TGTACCCAAG 
ATCACCAGCC TCCTGGACAA GCTGCATTCC ACCCGACAAC ACCTGCACCA AATGTGGCAC 
GTGCGAAAGC TCAAGCTGGA TCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAG 
AAGATGTTCG ACTGGATAAG CCACAACAAG GAGTTATTTC TTCAGAGCCA CACAGAGATC 
GGAGTCAGCT ACCAGCATGC ACTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC 
TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TCGCTTCCCG CCTCTCAGAG 
GCCGGCCATT ATGCCTCCCA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG 
AAGAGCTTTG CCGCTGCCCT GGATGAACGC AGTACAATCC TGGCCATGTC TGCTGTGTTC 
CACCAGAAGG CAGAGCAGTT CCTGTCAGGC GTGGATGCCT GGTGTAAGAT GTGCAGCGAA 
GGCGGCCTTC CTTCTGAGAT GCAGGACCTG GAGCTGGCAA TCCACCATCA CCAGTCCTTA 
TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ATGGCAAAGC ACTACTGGAT 
GTCCTGCAGC GTCCCCTGAG TCCTGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC 
TCCAAGGCGG TCCACCAGGT GCTGGATGTG GTCCACGAGG TGCTGCACCA TCAGCGACGG 
CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTTCACC AGCGGCTGCA GCTGTGTGTC 
TTCCAGCAGG ATGTGCAGCA GGTGCTGGAC TGGATTGAAA ACCATGGCGA GGCCTTTCTC 
AGCAAACACA CTGGGGTTGG CAAGTCCTTG CATCGAGCAC GTGCACTACA GAAGAGGCAT 
GATGACTTTG AAGAAGTGGC ACAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCA 
GCGGAGCAGC TGGCTCAGAC TGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCCCGG 
CACCTGGAGG TGCGCATCCA AGACTTTGTG CGCAGAGTGG AGCAGCGGAA GCTGCTTCTG 
GACATGTCGG TCTCTTTCCA CACGCACACC AAAGAGCTGT GGACATGGAT GGAGGACCTT 
CAGAAGGAGG TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAGCTGATC 
AAGCAGTTCC AGCAGCAGCA GACCGCCACC CTGGATGCCA CTCTCAATGT CATCAAGGAA 
GGCGAAGACC TTATCCAGCA GCTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCACCGAG 
GCCAGGGACT CAGCGATGTC CAACAACAAG ACACCCCACA GTAGCTCTAT CAGCCATATT 
GAGTCTGTCC TCCAGCAGCT GGACGATGCT CAGGTACAGA TGGAAGAGCT GTTTCACGAG 
CGGAAGATCA AGCTGGACAT CTTCCTACAG CTGCGCATCT TTGAACAGTA CACCATTGAG 
GTCACTGCAG AGCTGGATGC CTGGAACGAG GACTTGCTCC GGCAGATGAA TGATTTCAAC 
ACAGAGGATC TGACCCTGGC TGAGCAGCGG CTACAGCGCC ACACAGAACG GAAGCTTGCC 
ATGAACAACA TGACGTTCGA GGTCATCCAG CAGGGCCAGG ACCTGCACCA GTACATCATG 
GAAGTGCAAG CGTCAGGAAT CGAGTTAATC TGTGAGAAGG ACCTTGACCT GGCAGCTCAG 
GTGCAGGAGC TGCTGGAGTT TCTCCATGAG AAGCAGCACG AGCTGGAGCT CAATGCGGAG 
CAGACGCACA AGCGGCTAGA GCAGTGTCTC CAGCTGAGGC ACCTCCAGGC TGAGGTCAAA 
CAGGTTCTGG GATGGATCCG CAATGGAGAG TCGATGCTCA ACGCCAGCCT GGTCAACGCC 
AGCTCTTTGT CGGAGGCAGA GCAGCTGCAG CGGGAGCATG AACAGTTCCA GCTAGCCATC 
GAGAAGACGC ACCAGAGCGC CCTGCAGGTA CAGCAGAAGG CAGAGGCACT GCTCCAGGCC 
GGCCACTACG ACGCAGACGC CATCCGGGAA TGTGCTGAGA AGGTGGCCCT CCACTGGCAA 
CAGCTCATGC TAAAGATGGA GGACCGGCTG AAGCTTGTCA ACGCCTCTGT GGCCTTTTAC 
AAGACCTCTG AGCAGGTGTG CAGTGTTCTG GAGAGCTTGG AGCAGGAGTA CCGGAGAGAC 
GAGGACTGGT GTGGTGGGAG AGACAAGCTG GGGCCGGCGG CAGAGATGGA CCACGTTATT 
CCACTCCTCA GCAAGCACCT GGAGCAGAAG GAGGCCTTCC TGAAGGCCTG CACGCTGGCA 
AGGCGGAACG CCGAGGTGTT CCTCAAATAC ATCCACAGGA ACAATGTGAG CATGCCTAGT 
GTTGCCAGCC ACACCCGGGG GCCTGAACAG CAAGTAAAAG CCATCTTGAG CGAGCTCTTG 
CAGAGGGAGA ATCGAGTCCT GCACTTCTGG ACCTTGAAGA AGCGGCGGTT AGACCAGTGT 
CAGCAATATG TGGTGTTTGA GCGCAGTGCT AAGCAGGCAC TGGACTGGAT CCAAGAAACA 
GGAGAATATT ACCTCTCAAC ACATACCTCC ACCGGAGAGA CCACGGAGGA GACTCAGGAG 
CTGCTGAAAG AGTATGGAGA ATTCAGGGTG CCTGCCAAGC AAACGAAGGA GAAGGTGAAA 
CTTCTGATCC AGCTAGCAGA CAGCTTTGTG GAAAAGGGCC ACATCCACGC CACAGAGATA 
CGGAAATGGG TGACCACGGT GGACAAGCAC TACCGCGATT TCTCCCTGAG GATGGGGAAG 
TACCGGTACT CGCTGGAGAA GGCGCTGGGG GTCAACACGG AGGATAACAA GGACCTAGAG 
CTGGACATCA TCCCAGCAAG CCTTTCGGAT CGGGAGGTCA AGCTCCGGGA CGCTAACCAT 
GAGATCAATG AAGAGAAACG GAAGTCAGCT CGAAAGAAAG AGTTCATTAT GGCTGAACTG 
CTCCAAACTG AGAAGGCTTA TGTAAGGGAC TTGCACGAGT GCTTGGAGAC CTACCTGTGG 
GAGATGACTA GTGGTGTGGA AGAGATACCC CCCGGCATCC TCAATAAGGA ACATATCATC 
TTCGGCAACA TCCAGGAGAT CTATGACTTC CATAACAACA TCTTCCTCAA AGAGCTGGAG 
AAGTACGAGC AGCTGCCTGA GGATGTGGGA CACTGCTTTG TTACCTGGGC AGACAAATTT 
CAGATGTATG TCACCTACTG TAAAAACAAG CCTGATTCCA ACCAGCTGAT CCTGGAGCAT 
GCGGGCACCT TCTTTGATGA GATCCAGCAG CGGCATGGCC TGGCCAACTC CATCTCCTCC 
TACCTAATTA AGCCTGTCCA GAGGGTTACC AAGTACCAGC TGCTCCTGAA GGAACTTCTA 
ACTTGCTGTG AAGAAGGGAA AGGAGAGCTG AAGGATGGCC TGGAGGTGAT GCTCAGTGTC 
CCCAAGAAAG CCAACGATGC CATGCATGTC AGCATGCTGG AAGGGTTCGA TGAAAACTTG 
GATGTACAGG GGGAGTTGAT TCTCCAGGAT GCCTTTCAAG TGTGGGACCC AAAGTCGCTG 
ATCCGGAAGG GGCGGGAGCG CCATCTGTTC CTCTTTGAGA TCTCCTTGGT GTTTAGCAAA 
GAGATCAAAG ACTCTTCAGG ACACACGAAA TATGTTTACA AGAACAAGCT ACTGACCTCA 
GAGCTGGGTG TGACCGAGCA TGTAGAAGGT GACCCCTGCA AATTCGCCTT GTGGTCTGGG 
CGCACCCCAT CCTCAGACAA TAAAACAGTG CTCAAAGCTT CCAACATCGA AACCAAGCAG 
GAATGGATCA AGAACATTCG AGAAGTGATT CAGGAGAGGA TCATTCATCT TAAAGGCGCT 
TTAAAGGAGC CAATTCAGCT CCCCAAAACA CCAGCCAAAC TGAGGAACAA TAGTAAGAGG 
GATGGGGTAG AGGATGGTGA CAGCCAAGGG GATGGGAGCA GCCAGCCAGA TACCATATCC 
ATTGCCTCCA GGACCTCTCA GAACACAGTG GAAAGTGACA AGGATGGCAA CCTTGTTCCT 
CGTTGGCACC TGGGACCTGG AGATCCTTTC TCCACTTACG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157740.1
CDS	48..5012
Translation	MTDRFWDQWYLWYLRLLRLLDRGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKDGNLVPRWHLGPGDPFSTYV

Target ORF information:

RefSeq Version	NM_001164268.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164268.1

ORF Insert Sequence:

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGAT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC TATCCTAAAG 
GAGAAAGTGG CCTTTGTGTC TGGGGGGCGT GACAAGCGAG GTGGACCCAT CCTGACCTTC 
CCTGCCCGAA GCAATCATGA CAGAATAAGA CAGGAAGACC TACGGAAACT TGTGACGTAT 
TTGGCCAGCG TGCCAAGTGA GGACGTTTGC AAACGTGGCT TCACCGTCAT CATCGACATG 
CGGGGCTCCA AGTGGGATCT CATCAAGCCC CTCCTCAAGA CGCTGCAGGA AGCCTTCCCC 
GCCGAGATCC ATGTGGCCCT CATCATCAAG CCTGACAACT TCTGGCAGAA GCAGAAGACC 
AACTTTGGCA GCTCCAAATT CATCTTTGAG ACAAGCATGG TGTCTGTGGA GGGCCTCACG 
AAGCTGGTGG ACCCCTCCCA GCTGACAGAG GAGTTTGACG GCTCCCTGGA TTACAACCAT 
GAGGAGTGGA TTGAGTTGCG CCTCTCCCTG GAGGAGTTCT TCAACAGTGC GGTCCACCTG 
CTCTCACGCC TTGAGGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC TGTGGATGTG 
GAGGGCTCTC GGAGGCTTAT CGACGAGCAC ACGCAACTTA AGAAAAAGGT GCTGAAGGCC 
CCCGTGGAAG AGCTGGACCG GGAGGGGCAG CGTCTACTGC AGTGCATCCG CTGCAGCGAT 
GGCTTCTCAG GGCGCAACTG CATCCCAGGA AGTGCCGACT TCCAGAGCCT TGTACCCAAG 
ATCACCAGCC TCCTGGACAA GCTGCATTCC ACCCGACAAC ACCTGCACCA AATGTGGCAC 
GTGCGAAAGC TCAAGCTGGA TCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAG 
AAGATGTTCG ACTGGATAAG CCACAACAAG GAGTTATTTC TTCAGAGCCA CACAGAGATC 
GGAGTCAGCT ACCAGCATGC ACTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC 
TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TCGCTTCCCG CCTCTCAGAG 
GCCGGCCATT ATGCCTCCCA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG 
AAGAGCTTTG CCGCTGCCCT GGATGAACGC AGTACAATCC TGGCCATGTC TGCTGTGTTC 
CACCAGAAGG CAGAGCAGTT CCTGTCAGGC GTGGATGCCT GGTGTAAGAT GTGCAGCGAA 
GGCGGCCTTC CTTCTGAGAT GCAGGACCTG GAGCTGGCAA TCCACCATCA CCAGTCCTTA 
TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ATGGCAAAGC ACTACTGGAT 
GTCCTGCAGC GTCCCCTGAG TCCTGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC 
TCCAAGGCGG TCCACCAGGT GCTGGATGTG GTCCACGAGG TGCTGCACCA TCAGCGACGG 
CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTTCACC AGCGGCTGCA GCTGTGTGTC 
TTCCAGCAGG ATGTGCAGCA GGTGCTGGAC TGGATTGAAA ACCATGGCGA GGCCTTTCTC 
AGCAAACACA CTGGGGTTGG CAAGTCCTTG CATCGAGCAC GTGCACTACA GAAGAGGCAT 
GATGACTTTG AAGAAGTGGC ACAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCA 
GCGGAGCAGC TGGCTCAGAC TGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCCCGG 
CACCTGGAGG TGCGCATCCA AGACTTTGTG CGCAGAGTGG AGCAGCGGAA GCTGCTTCTG 
GACATGTCGG TCTCTTTCCA CACGCACACC AAAGAGCTGT GGACATGGAT GGAGGACCTT 
CAGAAGGAGG TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAGCTGATC 
AAGCAGTTCC AGCAGCAGCA GACCGCCACC CTGGATGCCA CTCTCAATGT CATCAAGGAA 
GGCGAAGACC TTATCCAGCA GCTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCACCGAG 
GCCAGGGACT CAGCGATGTC CAACAACAAG ACACCCCACA GTAGCTCTAT CAGCCATATT 
GAGTCTGTCC TCCAGCAGCT GGACGATGCT CAGGTACAGA TGGAAGAGCT GTTTCACGAG 
CGGAAGATCA AGCTGGACAT CTTCCTACAG CTGCGCATCT TTGAACAGTA CACCATTGAG 
GTCACTGCAG AGCTGGATGC CTGGAACGAG GACTTGCTCC GGCAGATGAA TGATTTCAAC 
ACAGAGGATC TGACCCTGGC TGAGCAGCGG CTACAGCGCC ACACAGAACG GAAGCTTGCC 
ATGAACAACA TGACGTTCGA GGTCATCCAG CAGGGCCAGG ACCTGCACCA GTACATCATG 
GAAGTGCAAG CGTCAGGAAT CGAGTTAATC TGTGAGAAGG ACCTTGACCT GGCAGCTCAG 
GTGCAGGAGC TGCTGGAGTT TCTCCATGAG AAGCAGCACG AGCTGGAGCT CAATGCGGAG 
CAGACGCACA AGCGGCTAGA GCAGTGTCTC CAGCTGAGGC ACCTCCAGGC TGAGGTCAAA 
CAGGTTCTGG GATGGATCCG CAATGGAGAG TCGATGCTCA ACGCCAGCCT GGTCAACGCC 
AGCTCTTTGT CGGAGGCAGA GCAGCTGCAG CGGGAGCATG AACAGTTCCA GCTAGCCATC 
GAGAAGACGC ACCAGAGCGC CCTGCAGGTA CAGCAGAAGG CAGAGGCACT GCTCCAGGCC 
GGCCACTACG ACGCAGACGC CATCCGGGAA TGTGCTGAGA AGGTGGCCCT CCACTGGCAA 
CAGCTCATGC TAAAGATGGA GGACCGGCTG AAGCTTGTCA ACGCCTCTGT GGCCTTTTAC 
AAGACCTCTG AGCAGGTGTG CAGTGTTCTG GAGAGCTTGG AGCAGGAGTA CCGGAGAGAC 
GAGGACTGGT GTGGTGGGAG AGACAAGCTG GGGCCGGCGG CAGAGATGGA CCACGTTATT 
CCACTCCTCA GCAAGCACCT GGAGCAGAAG GAGGCCTTCC TGAAGGCCTG CACGCTGGCA 
AGGCGGAACG CCGAGGTGTT CCTCAAATAC ATCCACAGGA ACAATGTGAG CATGCCTAGT 
GTTGCCAGCC ACACCCGGGG GCCTGAACAG CAAGTAAAAG CCATCTTGAG CGAGCTCTTG 
CAGAGGGAGA ATCGAGTCCT GCACTTCTGG ACCTTGAAGA AGCGGCGGTT AGACCAGTGT 
CAGCAATATG TGGTGTTTGA GCGCAGTGCT AAGCAGGCAC TGGACTGGAT CCAAGAAACA 
GGAGAATATT ACCTCTCAAC ACATACCTCC ACCGGAGAGA CCACGGAGGA GACTCAGGAG 
CTGCTGAAAG AGTATGGAGA ATTCAGGGTG CCTGCCAAGC AAACGAAGGA GAAGGTGAAA 
CTTCTGATCC AGCTAGCAGA CAGCTTTGTG GAAAAGGGCC ACATCCACGC CACAGAGATA 
CGGAAATGGG TGACCACGGT GGACAAGCAC TACCGCGATT TCTCCCTGAG GATGGGGAAG 
TACCGGTACT CGCTGGAGAA GGCGCTGGGG GTCAACACGG AGGATAACAA GGACCTAGAG 
CTGGACATCA TCCCAGCAAG CCTTTCGGAT CGGGAGGTCA AGCTCCGGGA CGCTAACCAT 
GAGATCAATG AAGAGAAACG GAAGTCAGCT CGAAAGAAAG AGTTCATTAT GGCTGAACTG 
CTCCAAACTG AGAAGGCTTA TGTAAGGGAC TTGCACGAGT GCTTGGAGAC CTACCTGTGG 
GAGATGACTA GTGGTGTGGA AGAGATACCC CCCGGCATCC TCAATAAGGA ACATATCATC 
TTCGGCAACA TCCAGGAGAT CTATGACTTC CATAACAACA TCTTCCTCAA AGAGCTGGAG 
AAGTACGAGC AGCTGCCTGA GGATGTGGGA CACTGCTTTG TTACCTGGGC AGACAAATTT 
CAGATGTATG TCACCTACTG TAAAAACAAG CCTGATTCCA ACCAGCTGAT CCTGGAGCAT 
GCGGGCACCT TCTTTGATGA GATCCAGCAG CGGCATGGCC TGGCCAACTC CATCTCCTCC 
TACCTAATTA AGCCTGTCCA GAGGGTTACC AAGTACCAGC TGCTCCTGAA GGAACTTCTA 
ACTTGCTGTG AAGAAGGGAA AGGAGAGCTG AAGGATGGCC TGGAGGTGAT GCTCAGTGTC 
CCCAAGAAAG CCAACGATGC CATGCATGTC AGCATGCTGG AAGGGTTCGA TGAAAACTTG 
GATGTACAGG GGGAGTTGAT TCTCCAGGAT GCCTTTCAAG TGTGGGACCC AAAGTCGCTG 
ATCCGGAAGG GGCGGGAGCG CCATCTGTTC CTCTTTGAGA TCTCCTTGGT GTTTAGCAAA 
GAGATCAAAG ACTCTTCAGG ACACACGAAA TATGTTTACA AGAACAAGCT ACTGACCTCA 
GAGCTGGGTG TGACCGAGCA TGTAGAAGGT GACCCCTGCA AATTCGCCTT GTGGTCTGGG 
CGCACCCCAT CCTCAGACAA TAAAACAGTG CTCAAAGCTT CCAACATCGA AACCAAGCAG 
GAATGGATCA AGAACATTCG AGAAGTGATT CAGGAGAGGA TCATTCATCT TAAAGGCGCT 
TTAAAGGAGC CAATTCAGCT CCCCAAAACA CCAGCCAAAC TGAGGAACAA TAGTAAGAGG 
GATGGGGTAG AGGATGGTGA CAGCCAAGGG GATGGGAGCA GCCAGCCAGA TACCATATCC 
ATTGCCTCCA GGACCTCTCA GAACACAGTG GAAAGTGACA AGGATGGCAA CCTTGTTCCT 
CGTTGGCACC TGGGACCTGG AGATCCTTTC TCCACTTACG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522382.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522445.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X28, mRNA.

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CloneID	OMu52298
Clone ID Related Accession (Same CDS sequence)	XM_006522382.3 , XM_006522382.4
Accession Version	XM_006522382.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522382.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGAT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC TATCCTAAAG 
GAGAAAGTGG CCTTTGTGTC TGGGGGGCGT GACAAGCGAG GTGGACCCAT CCTGACCTTC 
CCTGCCCGAA GCAATCATGA CAGAATAAGA CAGGAAGACC TACGGAAACT TGTGACGTAT 
TTGGCCAGCG TGCCAAGTGA GGACGTTTGC AAACGTGGCT TCACCGTCAT CATCGACATG 
CGGGGCTCCA AGTGGGATCT CATCAAGCCC CTCCTCAAGA CGCTGCAGGA AGCCTTCCCC 
GCCGAGATCC ATGTGGCCCT CATCATCAAG CCTGACAACT TCTGGCAGAA GCAGAAGACC 
AACTTTGGCA GCTCCAAATT CATCTTTGAG ACAAGCATGG TGTCTGTGGA GGGCCTCACG 
AAGCTGGTGG ACCCCTCCCA GCTGACAGAG GAGTTTGACG GCTCCCTGGA TTACAACCAT 
GAGGAGTGGA TTGAGTTGCG CCTCTCCCTG GAGGAGTTCT TCAACAGTGC GGTCCACCTG 
CTCTCACGCC TTGAGGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC TGTGGATGTG 
GAGGGCTCTC GGAGGCTTAT CGACGAGCAC ACGCAACTTA AGAAAAAGGT GCTGAAGGCC 
CCCGTGGAAG AGCTGGACCG GGAGGGGCAG CGTCTACTGC AGTGCATCCG CTGCAGCGAT 
GGCTTCTCAG GGCGCAACTG CATCCCAGGA AGTGCCGACT TCCAGAGCCT TGTACCCAAG 
ATCACCAGCC TCCTGGACAA GCTGCATTCC ACCCGACAAC ACCTGCACCA AATGTGGCAC 
GTGCGAAAGC TCAAGCTGGA TCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAG 
AAGATGTTCG ACTGGATAAG CCACAACAAG GAGTTATTTC TTCAGAGCCA CACAGAGATC 
GGAGTCAGCT ACCAGCATGC ACTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC 
TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TCGCTTCCCG CCTCTCAGAG 
GCCGGCCATT ATGCCTCCCA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG 
AAGAGCTTTG CCGCTGCCCT GGATGAACGC AGTACAATCC TGGCCATGTC TGCTGTGTTC 
CACCAGAAGG CAGAGCAGTT CCTGTCAGGC GTGGATGCCT GGTGTAAGAT GTGCAGCGAA 
GGCGGCCTTC CTTCTGAGAT GCAGGACCTG GAGCTGGCAA TCCACCATCA CCAGTCCTTA 
TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ATGGCAAAGC ACTACTGGAT 
GTCCTGCAGC GTCCCCTGAG TCCTGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC 
TCCAAGGCGG TCCACCAGGT GCTGGATGTG GTCCACGAGG TGCTGCACCA TCAGCGACGG 
CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTTCACC AGCGGCTGCA GCTGTGTGTC 
TTCCAGCAGG ATGTGCAGCA GGTGCTGGAC TGGATTGAAA ACCATGGCGA GGCCTTTCTC 
AGCAAACACA CTGGGGTTGG CAAGTCCTTG CATCGAGCAC GTGCACTACA GAAGAGGCAT 
GATGACTTTG AAGAAGTGGC ACAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCA 
GCGGAGCAGC TGGCTCAGAC TGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCCCGG 
CACCTGGAGG TGCGCATCCA AGACTTTGTG CGCAGAGTGG AGCAGCGGAA GCTGCTTCTG 
GACATGTCGG TCTCTTTCCA CACGCACACC AAAGAGCTGT GGACATGGAT GGAGGACCTT 
CAGAAGGAGG TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAGCTGATC 
AAGCAGTTCC AGCAGCAGCA GACCGCCACC CTGGATGCCA CTCTCAATGT CATCAAGGAA 
GGCGAAGACC TTATCCAGCA GCTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCACCGAG 
GCCAGGGACT CAGCGATGTC CAACAACAAG ACACCCCACA GTAGCTCTAT CAGCCATATT 
GAGTCTGTCC TCCAGCAGCT GGACGATGCT CAGGTACAGA TGGAAGAGCT GTTTCACGAG 
CGGAAGATCA AGCTGGACAT CTTCCTACAG CTGCGCATCT TTGAACAGTA CACCATTGAG 
GTCACTGCAG AGCTGGATGC CTGGAACGAG GACTTGCTCC GGCAGATGAA TGATTTCAAC 
ACAGAGGATC TGACCCTGGC TGAGCAGCGG CTACAGCGCC ACACAGAACG GAAGCTTGCC 
ATGAACAACA TGACGTTCGA GGTCATCCAG CAGGGCCAGG ACCTGCACCA GTACATCATG 
GAAGTGCAAG CGTCAGGAAT CGAGTTAATC TGTGAGAAGG ACCTTGACCT GGCAGCTCAG 
GTGCAGGAGC TGCTGGAGTT TCTCCATGAG AAGCAGCACG AGCTGGAGCT CAATGCGGAG 
CAGACGCACA AGCGGCTAGA GCAGTGTCTC CAGCTGAGGC ACCTCCAGGC TGAGGTCAAA 
CAGGTTCTGG GATGGATCCG CAATGGAGAG TCGATGCTCA ACGCCAGCCT GGTCAACGCC 
AGCTCTTTGT CGGAGGCAGA GCAGCTGCAG CGGGAGCATG AACAGTTCCA GCTAGCCATC 
GAGTCCCTCT TTCATGCCAC TTCCTTGCAG AAGACGCACC AGAGCGCCCT GCAGGTACAG 
CAGAAGGCAG AGGCACTGCT CCAGGCCGGC CACTACGACG CAGACGCCAT CCGGGAATGT 
GCTGAGAAGG TGGCCCTCCA CTGGCAACAG CTCATGCTAA AGATGGAGGA CCGGCTGAAG 
CTTGTCAACG CCTCTGTGGC CTTTTACAAG ACCTCTGAGC AGGTGTGCAG TGTTCTGGAG 
AGCTTGGAGC AGGAGTACCG GAGAGACGAG GACTGGTGTG GTGGGAGAGA CAAGCTGGGG 
CCGGCGGCAG AGATGGACCA CGTTATTCCA CTCCTCAGCA AGCACCTGGA GCAGAAGGAG 
GCCTTCCTGA AGGCCTGCAC GCTGGCAAGG CGGAACGCCG AGGTGTTCCT CAAATACATC 
CACAGGAACA ATGTGAGCAT GCCTAGTGTT GCCAGCCACA CCCGGGGGCC TGAACAGCAA 
GTAAAAGCCA TCTTGAGCGA GCTCTTGCAG AGGGAGAATC GAGTCCTGCA CTTCTGGACC 
TTGAAGAAGC GGCGGTTAGA CCAGTGTCAG CAATATGTGG TGTTTGAGCG CAGTGCTAAG 
CAGGCACTGG ACTGGATCCA AGAAACAGGA GAATATTACC TCTCAACACA TACCTCCACC 
GGAGAGACCA CGGAGGAGAC TCAGGAGCTG CTGAAAGAGT ATGGAGAATT CAGGGTGCCT 
GCCAAGCAAA CGAAGGAGAA GGTGAAACTT CTGATCCAGC TAGCAGACAG CTTTGTGGAA 
AAGGGCCACA TCCACGCCAC AGAGATACGG AAATGGGTGA CCACGGTGGA CAAGCACTAC 
CGCGATTTCT CCCTGAGGAT GGGGAAGTAC CGGTACTCGC TGGAGAAGGC GCTGGGGGTC 
AACACGGAGG ATAACAAGGA CCTAGAGCTG GACATCATCC CAGCAAGCCT TTCGGATCGG 
GAGGTCAAGC TCCGGGACGC TAACCATGAG ATCAATGAAG AGAAACGGAA GTCAGCTCGA 
AAGAAAGAGT TCATTATGGC TGAACTGCTC CAAACTGAGA AGGCTTATGT AAGGGACTTG 
CACGAGTGCT TGGAGACCTA CCTGTGGGAG ATGACTAGTG GTGTGGAAGA GATACCCCCC 
GGCATCCTCA ATAAGGAACA TATCATCTTC GGCAACATCC AGGAGATCTA TGACTTCCAT 
AACAACATCT TCCTCAAAGA GCTGGAGAAG TACGAGCAGC TGCCTGAGGA TGTGGGACAC 
TGCTTTGTTA CCTGGGCAGA CAAATTTCAG ATGTATGTCA CCTACTGTAA AAACAAGCCT 
GATTCCAACC AGCTGATCCT GGAGCATGCG GGCACCTTCT TTGATGAGAT CCAGCAGCGG 
CATGGCCTGG CCAACTCCAT CTCCTCCTAC CTAATTAAGC CTGTCCAGAG GGTTACCAAG 
TACCAGCTGC TCCTGAAGGA ACTTCTAACT TGCTGTGAAG AAGGGAAAGG AGAGCTGAAG 
GATGGCCTGG AGGTGATGCT CAGTGTCCCC AAGAAAGCCA ACGATGCCAT GCATGTCAGC 
ATGCTGGAAG GGTTCGATGA AAACTTGGAT GTACAGGGGG AGTTGATTCT CCAGGATGCC 
TTTCAAGTGT GGGACCCAAA GTCGCTGATC CGGAAGGGGC GGGAGCGCCA TCTGTTCCTC 
TTTGAGATCT CCTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACGAAATAT 
GTTTACAAGA ACAAGCTACT GACCTCAGAG CTGGGTGTGA CCGAGCATGT AGAAGGTGAC 
CCCTGCAAAT TCGCCTTGTG GTCTGGGCGC ACCCCATCCT CAGACAATAA AACAGTGCTC 
AAAGCTTCCA ACATCGAAAC CAAGCAGGAA TGGATCAAGA ACATTCGAGA AGTGATTCAG 
GAGAGGATCA TTCATCTTAA AGGCGCTTTA AAGGAGCCAA TTCAGCTCCC CAAAACACCA 
GCCAAACTGA GGAACAATAG TAAGAGGGAT GGGGTAGAGG ATGGTGACAG CCAAGGGGAT 
GGGAGCAGCC AGCCAGATAC CATATCCATT GCCTCCAGGA CCTCTCAGAA CACAGTGGAA 
AGTGACAAGG ATGGCAACCT TGTTCCTCGT TGGCACCTGG GACCTGGAGA TCCTTTCTCC 
ACTTACGTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522445.1
CDS	3331..8322
Translation	MTDRFWDQWYLWYLRLLRLLDRGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKDGNLVPRWHLGPGDPFSTYV

Target ORF information:

RefSeq Version	XM_006522382.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522382.3

ORF Insert Sequence:

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGAT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC TATCCTAAAG 
GAGAAAGTGG CCTTTGTGTC TGGGGGGCGT GACAAGCGAG GTGGACCCAT CCTGACCTTC 
CCTGCCCGAA GCAATCATGA CAGAATAAGA CAGGAAGACC TACGGAAACT TGTGACGTAT 
TTGGCCAGCG TGCCAAGTGA GGACGTTTGC AAACGTGGCT TCACCGTCAT CATCGACATG 
CGGGGCTCCA AGTGGGATCT CATCAAGCCC CTCCTCAAGA CGCTGCAGGA AGCCTTCCCC 
GCCGAGATCC ATGTGGCCCT CATCATCAAG CCTGACAACT TCTGGCAGAA GCAGAAGACC 
AACTTTGGCA GCTCCAAATT CATCTTTGAG ACAAGCATGG TGTCTGTGGA GGGCCTCACG 
AAGCTGGTGG ACCCCTCCCA GCTGACAGAG GAGTTTGACG GCTCCCTGGA TTACAACCAT 
GAGGAGTGGA TTGAGTTGCG CCTCTCCCTG GAGGAGTTCT TCAACAGTGC GGTCCACCTG 
CTCTCACGCC TTGAGGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC TGTGGATGTG 
GAGGGCTCTC GGAGGCTTAT CGACGAGCAC ACGCAACTTA AGAAAAAGGT GCTGAAGGCC 
CCCGTGGAAG AGCTGGACCG GGAGGGGCAG CGTCTACTGC AGTGCATCCG CTGCAGCGAT 
GGCTTCTCAG GGCGCAACTG CATCCCAGGA AGTGCCGACT TCCAGAGCCT TGTACCCAAG 
ATCACCAGCC TCCTGGACAA GCTGCATTCC ACCCGACAAC ACCTGCACCA AATGTGGCAC 
GTGCGAAAGC TCAAGCTGGA TCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAG 
AAGATGTTCG ACTGGATAAG CCACAACAAG GAGTTATTTC TTCAGAGCCA CACAGAGATC 
GGAGTCAGCT ACCAGCATGC ACTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC 
TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TCGCTTCCCG CCTCTCAGAG 
GCCGGCCATT ATGCCTCCCA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG 
AAGAGCTTTG CCGCTGCCCT GGATGAACGC AGTACAATCC TGGCCATGTC TGCTGTGTTC 
CACCAGAAGG CAGAGCAGTT CCTGTCAGGC GTGGATGCCT GGTGTAAGAT GTGCAGCGAA 
GGCGGCCTTC CTTCTGAGAT GCAGGACCTG GAGCTGGCAA TCCACCATCA CCAGTCCTTA 
TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ATGGCAAAGC ACTACTGGAT 
GTCCTGCAGC GTCCCCTGAG TCCTGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC 
TCCAAGGCGG TCCACCAGGT GCTGGATGTG GTCCACGAGG TGCTGCACCA TCAGCGACGG 
CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTTCACC AGCGGCTGCA GCTGTGTGTC 
TTCCAGCAGG ATGTGCAGCA GGTGCTGGAC TGGATTGAAA ACCATGGCGA GGCCTTTCTC 
AGCAAACACA CTGGGGTTGG CAAGTCCTTG CATCGAGCAC GTGCACTACA GAAGAGGCAT 
GATGACTTTG AAGAAGTGGC ACAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCA 
GCGGAGCAGC TGGCTCAGAC TGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCCCGG 
CACCTGGAGG TGCGCATCCA AGACTTTGTG CGCAGAGTGG AGCAGCGGAA GCTGCTTCTG 
GACATGTCGG TCTCTTTCCA CACGCACACC AAAGAGCTGT GGACATGGAT GGAGGACCTT 
CAGAAGGAGG TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAGCTGATC 
AAGCAGTTCC AGCAGCAGCA GACCGCCACC CTGGATGCCA CTCTCAATGT CATCAAGGAA 
GGCGAAGACC TTATCCAGCA GCTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCACCGAG 
GCCAGGGACT CAGCGATGTC CAACAACAAG ACACCCCACA GTAGCTCTAT CAGCCATATT 
GAGTCTGTCC TCCAGCAGCT GGACGATGCT CAGGTACAGA TGGAAGAGCT GTTTCACGAG 
CGGAAGATCA AGCTGGACAT CTTCCTACAG CTGCGCATCT TTGAACAGTA CACCATTGAG 
GTCACTGCAG AGCTGGATGC CTGGAACGAG GACTTGCTCC GGCAGATGAA TGATTTCAAC 
ACAGAGGATC TGACCCTGGC TGAGCAGCGG CTACAGCGCC ACACAGAACG GAAGCTTGCC 
ATGAACAACA TGACGTTCGA GGTCATCCAG CAGGGCCAGG ACCTGCACCA GTACATCATG 
GAAGTGCAAG CGTCAGGAAT CGAGTTAATC TGTGAGAAGG ACCTTGACCT GGCAGCTCAG 
GTGCAGGAGC TGCTGGAGTT TCTCCATGAG AAGCAGCACG AGCTGGAGCT CAATGCGGAG 
CAGACGCACA AGCGGCTAGA GCAGTGTCTC CAGCTGAGGC ACCTCCAGGC TGAGGTCAAA 
CAGGTTCTGG GATGGATCCG CAATGGAGAG TCGATGCTCA ACGCCAGCCT GGTCAACGCC 
AGCTCTTTGT CGGAGGCAGA GCAGCTGCAG CGGGAGCATG AACAGTTCCA GCTAGCCATC 
GAGTCCCTCT TTCATGCCAC TTCCTTGCAG AAGACGCACC AGAGCGCCCT GCAGGTACAG 
CAGAAGGCAG AGGCACTGCT CCAGGCCGGC CACTACGACG CAGACGCCAT CCGGGAATGT 
GCTGAGAAGG TGGCCCTCCA CTGGCAACAG CTCATGCTAA AGATGGAGGA CCGGCTGAAG 
CTTGTCAACG CCTCTGTGGC CTTTTACAAG ACCTCTGAGC AGGTGTGCAG TGTTCTGGAG 
AGCTTGGAGC AGGAGTACCG GAGAGACGAG GACTGGTGTG GTGGGAGAGA CAAGCTGGGG 
CCGGCGGCAG AGATGGACCA CGTTATTCCA CTCCTCAGCA AGCACCTGGA GCAGAAGGAG 
GCCTTCCTGA AGGCCTGCAC GCTGGCAAGG CGGAACGCCG AGGTGTTCCT CAAATACATC 
CACAGGAACA ATGTGAGCAT GCCTAGTGTT GCCAGCCACA CCCGGGGGCC TGAACAGCAA 
GTAAAAGCCA TCTTGAGCGA GCTCTTGCAG AGGGAGAATC GAGTCCTGCA CTTCTGGACC 
TTGAAGAAGC GGCGGTTAGA CCAGTGTCAG CAATATGTGG TGTTTGAGCG CAGTGCTAAG 
CAGGCACTGG ACTGGATCCA AGAAACAGGA GAATATTACC TCTCAACACA TACCTCCACC 
GGAGAGACCA CGGAGGAGAC TCAGGAGCTG CTGAAAGAGT ATGGAGAATT CAGGGTGCCT 
GCCAAGCAAA CGAAGGAGAA GGTGAAACTT CTGATCCAGC TAGCAGACAG CTTTGTGGAA 
AAGGGCCACA TCCACGCCAC AGAGATACGG AAATGGGTGA CCACGGTGGA CAAGCACTAC 
CGCGATTTCT CCCTGAGGAT GGGGAAGTAC CGGTACTCGC TGGAGAAGGC GCTGGGGGTC 
AACACGGAGG ATAACAAGGA CCTAGAGCTG GACATCATCC CAGCAAGCCT TTCGGATCGG 
GAGGTCAAGC TCCGGGACGC TAACCATGAG ATCAATGAAG AGAAACGGAA GTCAGCTCGA 
AAGAAAGAGT TCATTATGGC TGAACTGCTC CAAACTGAGA AGGCTTATGT AAGGGACTTG 
CACGAGTGCT TGGAGACCTA CCTGTGGGAG ATGACTAGTG GTGTGGAAGA GATACCCCCC 
GGCATCCTCA ATAAGGAACA TATCATCTTC GGCAACATCC AGGAGATCTA TGACTTCCAT 
AACAACATCT TCCTCAAAGA GCTGGAGAAG TACGAGCAGC TGCCTGAGGA TGTGGGACAC 
TGCTTTGTTA CCTGGGCAGA CAAATTTCAG ATGTATGTCA CCTACTGTAA AAACAAGCCT 
GATTCCAACC AGCTGATCCT GGAGCATGCG GGCACCTTCT TTGATGAGAT CCAGCAGCGG 
CATGGCCTGG CCAACTCCAT CTCCTCCTAC CTAATTAAGC CTGTCCAGAG GGTTACCAAG 
TACCAGCTGC TCCTGAAGGA ACTTCTAACT TGCTGTGAAG AAGGGAAAGG AGAGCTGAAG 
GATGGCCTGG AGGTGATGCT CAGTGTCCCC AAGAAAGCCA ACGATGCCAT GCATGTCAGC 
ATGCTGGAAG GGTTCGATGA AAACTTGGAT GTACAGGGGG AGTTGATTCT CCAGGATGCC 
TTTCAAGTGT GGGACCCAAA GTCGCTGATC CGGAAGGGGC GGGAGCGCCA TCTGTTCCTC 
TTTGAGATCT CCTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACGAAATAT 
GTTTACAAGA ACAAGCTACT GACCTCAGAG CTGGGTGTGA CCGAGCATGT AGAAGGTGAC 
CCCTGCAAAT TCGCCTTGTG GTCTGGGCGC ACCCCATCCT CAGACAATAA AACAGTGCTC 
AAAGCTTCCA ACATCGAAAC CAAGCAGGAA TGGATCAAGA ACATTCGAGA AGTGATTCAG 
GAGAGGATCA TTCATCTTAA AGGCGCTTTA AAGGAGCCAA TTCAGCTCCC CAAAACACCA 
GCCAAACTGA GGAACAATAG TAAGAGGGAT GGGGTAGAGG ATGGTGACAG CCAAGGGGAT 
GGGAGCAGCC AGCCAGATAC CATATCCATT GCCTCCAGGA CCTCTCAGAA CACAGTGGAA 
AGTGACAAGG ATGGCAACCT TGTTCCTCGT TGGCACCTGG GACCTGGAGA TCCTTTCTCC 
ACTTACGTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522383.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522446.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X29, mRNA.

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CloneID	OMu52299
Clone ID Related Accession (Same CDS sequence)	XM_006522383.4 , XM_006522383.3
Accession Version	XM_006522383.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522383.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGAAG ACGCACCAGA GCGCCCTGCA GGTACAGCAG 
AAGGCAGAGG CACTGCTCCA GGCCGGCCAC TACGACGCAG ACGCCATCCG GGAATGTGCT 
GAGAAGGTGG CCCTCCACTG GCAACAGCTC ATGCTAAAGA TGGAGGACCG GCTGAAGCTT 
GTCAACGCCT CTGTGGCCTT TTACAAGACC TCTGAGCAGG TGTGCAGTGT TCTGGAGAGC 
TTGGAGCAGG AGTACCGGAG AGACGAGGAC TGGTGTGGTG GGAGAGACAA GCTGGGGCCG 
GCGGCAGAGA TGGACCACGT TATTCCACTC CTCAGCAAGC ACCTGGAGCA GAAGGAGGCC 
TTCCTGAAGG CCTGCACGCT GGCAAGGCGG AACGCCGAGG TGTTCCTCAA ATACATCCAC 
AGGAACAATG TGAGCATGCC TAGTGTTGCC AGCCACACCC GGGGGCCTGA ACAGCAAGTA 
AAAGCCATCT TGAGCGAGCT CTTGCAGAGG GAGAATCGAG TCCTGCACTT CTGGACCTTG 
AAGAAGCGGC GGTTAGACCA GTGTCAGCAA TATGTGGTGT TTGAGCGCAG TGCTAAGCAG 
GCACTGGACT GGATCCAAGA AACAGGAGAA TATTACCTCT CAACACATAC CTCCACCGGA 
GAGACCACGG AGGAGACTCA GGAGCTGCTG AAAGAGTATG GAGAATTCAG GGTGCCTGCC 
AAGCAAACGA AGGAGAAGGT GAAACTTCTG ATCCAGCTAG CAGACAGCTT TGTGGAAAAG 
GGCCACATCC ACGCCACAGA GATACGGAAA TGGGTGACCA CGGTGGACAA GCACTACCGC 
GATTTCTCCC TGAGGATGGG GAAGTACCGG TACTCGCTGG AGAAGGCGCT GGGGGTCAAC 
ACGGAGGATA ACAAGGACCT AGAGCTGGAC ATCATCCCAG CAAGCCTTTC GGATCGGGAG 
GTCAAGCTCC GGGACGCTAA CCATGAGATC AATGAAGAGA AACGGAAGTC AGCTCGAAAG 
AAAGAGTTCA TTATGGCTGA ACTGCTCCAA ACTGAGAAGG CTTATGTAAG GGACTTGCAC 
GAGTGCTTGG AGACCTACCT GTGGGAGATG ACTAGTGGTG TGGAAGAGAT ACCCCCCGGC 
ATCCTCAATA AGGAACATAT CATCTTCGGC AACATCCAGG AGATCTATGA CTTCCATAAC 
AACATCTTCC TCAAAGAGCT GGAGAAGTAC GAGCAGCTGC CTGAGGATGT GGGACACTGC 
TTTGTTACCT GGGCAGACAA ATTTCAGATG TATGTCACCT ACTGTAAAAA CAAGCCTGAT 
TCCAACCAGC TGATCCTGGA GCATGCGGGC ACCTTCTTTG ATGAGATCCA GCAGCGGCAT 
GGCCTGGCCA ACTCCATCTC CTCCTACCTA ATTAAGCCTG TCCAGAGGGT TACCAAGTAC 
CAGCTGCTCC TGAAGGAACT TCTAACTTGC TGTGAAGAAG GGAAAGGAGA GCTGAAGGAT 
GGCCTGGAGG TGATGCTCAG TGTCCCCAAG AAAGCCAACG ATGCCATGCA TGTCAGCATG 
CTGGAAGGGT TCGATGAAAA CTTGGATGTA CAGGGGGAGT TGATTCTCCA GGATGCCTTT 
CAAGTGTGGG ACCCAAAGTC GCTGATCCGG AAGGGGCGGG AGCGCCATCT GTTCCTCTTT 
GAGATCTCCT TGGTGTTTAG CAAAGAGATC AAAGACTCTT CAGGACACAC GAAATATGTT 
TACAAGAACA AGCTACTGAC CTCAGAGCTG GGTGTGACCG AGCATGTAGA AGGTGACCCC 
TGCAAATTCG CCTTGTGGTC TGGGCGCACC CCATCCTCAG ACAATAAAAC AGTGCTCAAA 
GCTTCCAACA TCGAAACCAA GCAGGAATGG ATCAAGAACA TTCGAGAAGT GATTCAGGAG 
AGGATCATTC ATCTTAAAGG CGCTTTAAAG GAGCCAATTC AGCTCCCCAA AACACCAGCC 
AAACTGAGGA ACAATAGTAA GAGGGATGGG GTAGAGGATG GTGACAGCCA AGGGGATGGG 
AGCAGCCAGC CAGATACCAT ATCCATTGCC TCCAGGACCT CTCAGAACAC AGTGGAAAGT 
GACAAGGATG GCAACCTTGT TCCTCGTTGG CACCTGGGAC CTGGAGATCC TTTCTCCACT 
TACGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522446.1
CDS	4891..9879
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKDGNLVPRWHLGPGDPFSTYV

Target ORF information:

RefSeq Version	XM_006522383.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522383.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGAAG ACGCACCAGA GCGCCCTGCA GGTACAGCAG 
AAGGCAGAGG CACTGCTCCA GGCCGGCCAC TACGACGCAG ACGCCATCCG GGAATGTGCT 
GAGAAGGTGG CCCTCCACTG GCAACAGCTC ATGCTAAAGA TGGAGGACCG GCTGAAGCTT 
GTCAACGCCT CTGTGGCCTT TTACAAGACC TCTGAGCAGG TGTGCAGTGT TCTGGAGAGC 
TTGGAGCAGG AGTACCGGAG AGACGAGGAC TGGTGTGGTG GGAGAGACAA GCTGGGGCCG 
GCGGCAGAGA TGGACCACGT TATTCCACTC CTCAGCAAGC ACCTGGAGCA GAAGGAGGCC 
TTCCTGAAGG CCTGCACGCT GGCAAGGCGG AACGCCGAGG TGTTCCTCAA ATACATCCAC 
AGGAACAATG TGAGCATGCC TAGTGTTGCC AGCCACACCC GGGGGCCTGA ACAGCAAGTA 
AAAGCCATCT TGAGCGAGCT CTTGCAGAGG GAGAATCGAG TCCTGCACTT CTGGACCTTG 
AAGAAGCGGC GGTTAGACCA GTGTCAGCAA TATGTGGTGT TTGAGCGCAG TGCTAAGCAG 
GCACTGGACT GGATCCAAGA AACAGGAGAA TATTACCTCT CAACACATAC CTCCACCGGA 
GAGACCACGG AGGAGACTCA GGAGCTGCTG AAAGAGTATG GAGAATTCAG GGTGCCTGCC 
AAGCAAACGA AGGAGAAGGT GAAACTTCTG ATCCAGCTAG CAGACAGCTT TGTGGAAAAG 
GGCCACATCC ACGCCACAGA GATACGGAAA TGGGTGACCA CGGTGGACAA GCACTACCGC 
GATTTCTCCC TGAGGATGGG GAAGTACCGG TACTCGCTGG AGAAGGCGCT GGGGGTCAAC 
ACGGAGGATA ACAAGGACCT AGAGCTGGAC ATCATCCCAG CAAGCCTTTC GGATCGGGAG 
GTCAAGCTCC GGGACGCTAA CCATGAGATC AATGAAGAGA AACGGAAGTC AGCTCGAAAG 
AAAGAGTTCA TTATGGCTGA ACTGCTCCAA ACTGAGAAGG CTTATGTAAG GGACTTGCAC 
GAGTGCTTGG AGACCTACCT GTGGGAGATG ACTAGTGGTG TGGAAGAGAT ACCCCCCGGC 
ATCCTCAATA AGGAACATAT CATCTTCGGC AACATCCAGG AGATCTATGA CTTCCATAAC 
AACATCTTCC TCAAAGAGCT GGAGAAGTAC GAGCAGCTGC CTGAGGATGT GGGACACTGC 
TTTGTTACCT GGGCAGACAA ATTTCAGATG TATGTCACCT ACTGTAAAAA CAAGCCTGAT 
TCCAACCAGC TGATCCTGGA GCATGCGGGC ACCTTCTTTG ATGAGATCCA GCAGCGGCAT 
GGCCTGGCCA ACTCCATCTC CTCCTACCTA ATTAAGCCTG TCCAGAGGGT TACCAAGTAC 
CAGCTGCTCC TGAAGGAACT TCTAACTTGC TGTGAAGAAG GGAAAGGAGA GCTGAAGGAT 
GGCCTGGAGG TGATGCTCAG TGTCCCCAAG AAAGCCAACG ATGCCATGCA TGTCAGCATG 
CTGGAAGGGT TCGATGAAAA CTTGGATGTA CAGGGGGAGT TGATTCTCCA GGATGCCTTT 
CAAGTGTGGG ACCCAAAGTC GCTGATCCGG AAGGGGCGGG AGCGCCATCT GTTCCTCTTT 
GAGATCTCCT TGGTGTTTAG CAAAGAGATC AAAGACTCTT CAGGACACAC GAAATATGTT 
TACAAGAACA AGCTACTGAC CTCAGAGCTG GGTGTGACCG AGCATGTAGA AGGTGACCCC 
TGCAAATTCG CCTTGTGGTC TGGGCGCACC CCATCCTCAG ACAATAAAAC AGTGCTCAAA 
GCTTCCAACA TCGAAACCAA GCAGGAATGG ATCAAGAACA TTCGAGAAGT GATTCAGGAG 
AGGATCATTC ATCTTAAAGG CGCTTTAAAG GAGCCAATTC AGCTCCCCAA AACACCAGCC 
AAACTGAGGA ACAATAGTAA GAGGGATGGG GTAGAGGATG GTGACAGCCA AGGGGATGGG 
AGCAGCCAGC CAGATACCAT ATCCATTGCC TCCAGGACCT CTCAGAACAC AGTGGAAAGT 
GACAAGGATG GCAACCTTGT TCCTCGTTGG CACCTGGGAC CTGGAGATCC TTTCTCCACT 
TACGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245968.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244270.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X27, mRNA.

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CloneID	OMu75323
Clone ID Related Accession (Same CDS sequence)	XM_011245968.2 , XM_011245968.3
Accession Version	XM_011245968.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245968.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGGATGGCA ACCTTGTTCC TCGTTGGCAC 
CTGGGACCTG GAGATCCTTT CTCCACTTAC GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244270.1
CDS	4891..9906
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKDGNLVPRWHLGPGDPFSTYV

Target ORF information:

RefSeq Version	XM_011245968.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245968.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGGATGGCA ACCTTGTTCC TCGTTGGCAC 
CTGGGACCTG GAGATCCTTT CTCCACTTAC GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245969.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244271.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OMu75324
Clone ID Related Accession (Same CDS sequence)	XM_011245969.2
Accession Version	XM_011245969.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGGCATT TCCTGTCTAC 
AGACCTCAGA GCTGGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244271.1
CDS	4891..9531
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLAFPVYRPQSWV

Target ORF information:

RefSeq Version	XM_011245969.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245969.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGGCATT TCCTGTCTAC 
AGACCTCAGA GCTGGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245970.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244272.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X33, mRNA.

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CloneID	OMu75325
Clone ID Related Accession (Same CDS sequence)	XM_011245970.2 , XM_011245970.3
Accession Version	XM_011245970.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245970.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244272.1
CDS	4891..9501
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLP

Target ORF information:

RefSeq Version	XM_011245970.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245970.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245963.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244265.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X17, mRNA.

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CloneID	OMu75318
Clone ID Related Accession (Same CDS sequence)	XM_011245963.2 , XM_011245963.3
Accession Version	XM_011245963.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245963.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAGAAGTC ATGCTCTTGG CACACTCTAC GCATGAGAAA GCGGGCAGAC 
GTGGAGAACT CGGGTAAAAA TGAAGCCACA GGGCCTCGTA AACCCAAGGA TATTCTGGGC 
AACAAAGTCT CTGTTAAAAA ACAATGTGAT CATGATCCGC CTTCAGAAAT GCTTTGCCTT 
GGGACAATGA AAATCACTCA TTGGAGCTGG AGAGATGGCT CAGAGGTTAA GAGCACTGTC 
TGCTCTTCCA GAGGTCCTGA GTTTAATTCC CAGCAACCTT ATAGTGGCTC ACAACCATCT 
ATAATGAGAT CTGGTGCCCT CTTCTGGCTG CAGGCCATAA TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244265.1
CDS	24875..32419
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKKQCDHDPPSEMLCLGTMKITHWSWRDGSEVKSTVCSSRGPEFNSQQPYSGSQPSIMRSGALFWLQAII

Target ORF information:

RefSeq Version	XM_011245963.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245963.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAGAAGTC ATGCTCTTGG CACACTCTAC GCATGAGAAA GCGGGCAGAC 
GTGGAGAACT CGGGTAAAAA TGAAGCCACA GGGCCTCGTA AACCCAAGGA TATTCTGGGC 
AACAAAGTCT CTGTTAAAAA ACAATGTGAT CATGATCCGC CTTCAGAAAT GCTTTGCCTT 
GGGACAATGA AAATCACTCA TTGGAGCTGG AGAGATGGCT CAGAGGTTAA GAGCACTGTC 
TGCTCTTCCA GAGGTCCTGA GTTTAATTCC CAGCAACCTT ATAGTGGCTC ACAACCATCT 
ATAATGAGAT CTGGTGCCCT CTTCTGGCTG CAGGCCATAA TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245965.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244267.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X19, mRNA.

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CloneID	OMu75320
Clone ID Related Accession (Same CDS sequence)	XM_011245965.3 , XM_011245965.2
Accession Version	XM_011245965.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245965.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAAACCCT ACTGAAACCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244267.1
CDS	24875..32107
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKTLLKP

Target ORF information:

RefSeq Version	XM_011245965.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245965.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAAACCCT ACTGAAACCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245966.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244268.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X20, mRNA.

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CloneID	OMu75321
Clone ID Related Accession (Same CDS sequence)	XM_011245966.2 , XM_011245966.3
Accession Version	XM_011245966.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245966.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAAAAAGA ACCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244268.1
CDS	24875..32104
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKEPY

Target ORF information:

RefSeq Version	XM_011245966.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245966.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAAAAAGA ACCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245967.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244269.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X22, mRNA.

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CloneID	OMu75322
Clone ID Related Accession (Same CDS sequence)	XM_011245967.3 , XM_011245967.2
Accession Version	XM_011245967.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245967.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AAAAAGAACC CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244269.1
CDS	24875..32101
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKEPY

Target ORF information:

RefSeq Version	XM_011245967.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245967.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AAAAAGAACC CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317072.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172561.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X21, mRNA.

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CloneID	OMu79370
Clone ID Related Accession (Same CDS sequence)	XM_017317072.1 , XM_017317072.2
Accession Version	XM_017317072.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AAACCCTACT GAAACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172561.1
CDS	4891..12120
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKTLLKP

Target ORF information:

RefSeq Version	XM_017317072.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317072.1

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AAACCCTACT GAAACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317072.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172561.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X21, mRNA.

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CloneID	OMu79370
Clone ID Related Accession (Same CDS sequence)	XM_017317072.1 , XM_017317072.2
Accession Version	XM_017317072.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317072.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AAACCCTACT GAAACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172561.1
CDS	24875..32104
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKTLLKP

Target ORF information:

RefSeq Version	XM_017317072.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317072.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AAACCCTACT GAAACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317073.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172562.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X23, mRNA.

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CloneID	OMu79371
Clone ID Related Accession (Same CDS sequence)	XM_017317073.1 , XM_017317073.2
Accession Version	XM_017317073.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGTCGTCTT TGCTAGCAGC CCGGCAGGCT CCCCCTGACG TGCCTACTGC TGCAGACCTT 
GTCAGTGCAA TAGAAAAGTT GGTCAAAAAT AAGCTGACTC TGGAAGGAGG CTCTTACCGG 
GGGAGCTTGA AGGACCCTAC AGGCTGCCTG AATGAGGGGA TGACCCCTCC CACACCTCCT 
AGAAACCTGG AAGAAGAACA GAAAGCCAAG GCGCTGAGAG GCAGGATGTT TGTCCTGAAT 
GAGCTGGTAC AGACAGAGAA GGACTACGTC AAGGACCTGG GGATTGTGGT GGAGGGCTTC 
ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT GAGGACATGC GAGGGAAGGA AAAGATCGTG 
TTTGGAAATA TCCACCAGAT TTACGACTGG CATAAAGATT TTTTCCTGGC TGAACTGGAA 
AAATGTATCC AAGAGCAAGA CAGATTGGCC CAGCTCTTCA TTAAACACGA GCGGAAGCTG 
CACATCTACG TGTGGTACTG CCAGAACAAG CCGCGTTCCG AGTACATCGT GGCTGAGTAC 
GATGCCTATT TTGAGGAGGT TAAACAAGAG ATCAATCAAA GGCTGACCCT TAGTGACTTC 
CTGATCAAGC CCATTCAGAG AATAACAAAG TACCAGCTGC TACTCAAGGA CTTCCTGCGA 
TACAGCGAGA AGGCTGGTTT GGAGTGTTCC GATATTGAGA AAGCAGTGGA GTTAATGTGC 
CTTGTCCCCA AGCGCTGTAA TGACATGATG AATCTGGGAC GCCTGCAGGG CTTCGAGGGC 
ACCCTGACCG CCCAGGGCAA GCTACTGCAG CAGGACACAT TCTATGTGAT CGAGCTGGAT 
GCCGGCATGC AGTCCCGGAC CAAGGAGAGG CGTGTGTTTC TCTTTGAGCA GATTGTCATC 
TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC ACCCCAGGCT ACATGTTCAA AAGAAGCATC 
AAGATGAATT ACTTGGTCCT GGAAGACAAT GTGGATGGTG ACCCCTGCAA ATTTGCACTC 
ATGAACAGGG AGACTTCAGA AAGGGTCATT CTACAGGCTG CCAACTCAGA CATCCAGCAG 
GCCTGGGTGC AGGACATCAA CCAAGTCCTA GAAACACAGA GGGACTTCTT AAATGCACTA 
CAGTCACCCA TTGAGTATCA GCGGAAGGAG AGAAGCACAG CTGTGATCCG GTCCCAGCCC 
CCGAGGGTTC CACAAGCCAG CCCCAGGCCC TACTCTTCTG GCCCGGTGGG CTCAGAGAAG 
CCCCCCAAGG GCTCCAGCTA TAACCCGCCT CTGCCACCCC TGAAGATATC TACCTCTAAT 
GGCAGTCCAG GGTTCGACTA CCATCAGCCT GGGGACAAGT TTGACGCTAG CAAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172562.1
CDS	4891..12102
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIK

Target ORF information:

RefSeq Version	XM_017317073.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317073.1

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGTCGTCTT TGCTAGCAGC CCGGCAGGCT CCCCCTGACG TGCCTACTGC TGCAGACCTT 
GTCAGTGCAA TAGAAAAGTT GGTCAAAAAT AAGCTGACTC TGGAAGGAGG CTCTTACCGG 
GGGAGCTTGA AGGACCCTAC AGGCTGCCTG AATGAGGGGA TGACCCCTCC CACACCTCCT 
AGAAACCTGG AAGAAGAACA GAAAGCCAAG GCGCTGAGAG GCAGGATGTT TGTCCTGAAT 
GAGCTGGTAC AGACAGAGAA GGACTACGTC AAGGACCTGG GGATTGTGGT GGAGGGCTTC 
ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT GAGGACATGC GAGGGAAGGA AAAGATCGTG 
TTTGGAAATA TCCACCAGAT TTACGACTGG CATAAAGATT TTTTCCTGGC TGAACTGGAA 
AAATGTATCC AAGAGCAAGA CAGATTGGCC CAGCTCTTCA TTAAACACGA GCGGAAGCTG 
CACATCTACG TGTGGTACTG CCAGAACAAG CCGCGTTCCG AGTACATCGT GGCTGAGTAC 
GATGCCTATT TTGAGGAGGT TAAACAAGAG ATCAATCAAA GGCTGACCCT TAGTGACTTC 
CTGATCAAGC CCATTCAGAG AATAACAAAG TACCAGCTGC TACTCAAGGA CTTCCTGCGA 
TACAGCGAGA AGGCTGGTTT GGAGTGTTCC GATATTGAGA AAGCAGTGGA GTTAATGTGC 
CTTGTCCCCA AGCGCTGTAA TGACATGATG AATCTGGGAC GCCTGCAGGG CTTCGAGGGC 
ACCCTGACCG CCCAGGGCAA GCTACTGCAG CAGGACACAT TCTATGTGAT CGAGCTGGAT 
GCCGGCATGC AGTCCCGGAC CAAGGAGAGG CGTGTGTTTC TCTTTGAGCA GATTGTCATC 
TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC ACCCCAGGCT ACATGTTCAA AAGAAGCATC 
AAGATGAATT ACTTGGTCCT GGAAGACAAT GTGGATGGTG ACCCCTGCAA ATTTGCACTC 
ATGAACAGGG AGACTTCAGA AAGGGTCATT CTACAGGCTG CCAACTCAGA CATCCAGCAG 
GCCTGGGTGC AGGACATCAA CCAAGTCCTA GAAACACAGA GGGACTTCTT AAATGCACTA 
CAGTCACCCA TTGAGTATCA GCGGAAGGAG AGAAGCACAG CTGTGATCCG GTCCCAGCCC 
CCGAGGGTTC CACAAGCCAG CCCCAGGCCC TACTCTTCTG GCCCGGTGGG CTCAGAGAAG 
CCCCCCAAGG GCTCCAGCTA TAACCCGCCT CTGCCACCCC TGAAGATATC TACCTCTAAT 
GGCAGTCCAG GGTTCGACTA CCATCAGCCT GGGGACAAGT TTGACGCTAG CAAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317073.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172562.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X24, mRNA.

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CloneID	OMu79371
Clone ID Related Accession (Same CDS sequence)	XM_017317073.1 , XM_017317073.2
Accession Version	XM_017317073.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317073.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGTCGTCTT TGCTAGCAGC CCGGCAGGCT CCCCCTGACG TGCCTACTGC TGCAGACCTT 
GTCAGTGCAA TAGAAAAGTT GGTCAAAAAT AAGCTGACTC TGGAAGGAGG CTCTTACCGG 
GGGAGCTTGA AGGACCCTAC AGGCTGCCTG AATGAGGGGA TGACCCCTCC CACACCTCCT 
AGAAACCTGG AAGAAGAACA GAAAGCCAAG GCGCTGAGAG GCAGGATGTT TGTCCTGAAT 
GAGCTGGTAC AGACAGAGAA GGACTACGTC AAGGACCTGG GGATTGTGGT GGAGGGCTTC 
ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT GAGGACATGC GAGGGAAGGA AAAGATCGTG 
TTTGGAAATA TCCACCAGAT TTACGACTGG CATAAAGATT TTTTCCTGGC TGAACTGGAA 
AAATGTATCC AAGAGCAAGA CAGATTGGCC CAGCTCTTCA TTAAACACGA GCGGAAGCTG 
CACATCTACG TGTGGTACTG CCAGAACAAG CCGCGTTCCG AGTACATCGT GGCTGAGTAC 
GATGCCTATT TTGAGGAGGT TAAACAAGAG ATCAATCAAA GGCTGACCCT TAGTGACTTC 
CTGATCAAGC CCATTCAGAG AATAACAAAG TACCAGCTGC TACTCAAGGA CTTCCTGCGA 
TACAGCGAGA AGGCTGGTTT GGAGTGTTCC GATATTGAGA AAGCAGTGGA GTTAATGTGC 
CTTGTCCCCA AGCGCTGTAA TGACATGATG AATCTGGGAC GCCTGCAGGG CTTCGAGGGC 
ACCCTGACCG CCCAGGGCAA GCTACTGCAG CAGGACACAT TCTATGTGAT CGAGCTGGAT 
GCCGGCATGC AGTCCCGGAC CAAGGAGAGG CGTGTGTTTC TCTTTGAGCA GATTGTCATC 
TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC ACCCCAGGCT ACATGTTCAA AAGAAGCATC 
AAGATGAATT ACTTGGTCCT GGAAGACAAT GTGGATGGTG ACCCCTGCAA ATTTGCACTC 
ATGAACAGGG AGACTTCAGA AAGGGTCATT CTACAGGCTG CCAACTCAGA CATCCAGCAG 
GCCTGGGTGC AGGACATCAA CCAAGTCCTA GAAACACAGA GGGACTTCTT AAATGCACTA 
CAGTCACCCA TTGAGTATCA GCGGAAGGAG AGAAGCACAG CTGTGATCCG GTCCCAGCCC 
CCGAGGGTTC CACAAGCCAG CCCCAGGCCC TACTCTTCTG GCCCGGTGGG CTCAGAGAAG 
CCCCCCAAGG GCTCCAGCTA TAACCCGCCT CTGCCACCCC TGAAGATATC TACCTCTAAT 
GGCAGTCCAG GGTTCGACTA CCATCAGCCT GGGGACAAGT TTGACGCTAG CAAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172562.1
CDS	24875..32086
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIK

Target ORF information:

RefSeq Version	XM_017317073.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317073.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGTCGTCTT TGCTAGCAGC CCGGCAGGCT CCCCCTGACG TGCCTACTGC TGCAGACCTT 
GTCAGTGCAA TAGAAAAGTT GGTCAAAAAT AAGCTGACTC TGGAAGGAGG CTCTTACCGG 
GGGAGCTTGA AGGACCCTAC AGGCTGCCTG AATGAGGGGA TGACCCCTCC CACACCTCCT 
AGAAACCTGG AAGAAGAACA GAAAGCCAAG GCGCTGAGAG GCAGGATGTT TGTCCTGAAT 
GAGCTGGTAC AGACAGAGAA GGACTACGTC AAGGACCTGG GGATTGTGGT GGAGGGCTTC 
ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT GAGGACATGC GAGGGAAGGA AAAGATCGTG 
TTTGGAAATA TCCACCAGAT TTACGACTGG CATAAAGATT TTTTCCTGGC TGAACTGGAA 
AAATGTATCC AAGAGCAAGA CAGATTGGCC CAGCTCTTCA TTAAACACGA GCGGAAGCTG 
CACATCTACG TGTGGTACTG CCAGAACAAG CCGCGTTCCG AGTACATCGT GGCTGAGTAC 
GATGCCTATT TTGAGGAGGT TAAACAAGAG ATCAATCAAA GGCTGACCCT TAGTGACTTC 
CTGATCAAGC CCATTCAGAG AATAACAAAG TACCAGCTGC TACTCAAGGA CTTCCTGCGA 
TACAGCGAGA AGGCTGGTTT GGAGTGTTCC GATATTGAGA AAGCAGTGGA GTTAATGTGC 
CTTGTCCCCA AGCGCTGTAA TGACATGATG AATCTGGGAC GCCTGCAGGG CTTCGAGGGC 
ACCCTGACCG CCCAGGGCAA GCTACTGCAG CAGGACACAT TCTATGTGAT CGAGCTGGAT 
GCCGGCATGC AGTCCCGGAC CAAGGAGAGG CGTGTGTTTC TCTTTGAGCA GATTGTCATC 
TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC ACCCCAGGCT ACATGTTCAA AAGAAGCATC 
AAGATGAATT ACTTGGTCCT GGAAGACAAT GTGGATGGTG ACCCCTGCAA ATTTGCACTC 
ATGAACAGGG AGACTTCAGA AAGGGTCATT CTACAGGCTG CCAACTCAGA CATCCAGCAG 
GCCTGGGTGC AGGACATCAA CCAAGTCCTA GAAACACAGA GGGACTTCTT AAATGCACTA 
CAGTCACCCA TTGAGTATCA GCGGAAGGAG AGAAGCACAG CTGTGATCCG GTCCCAGCCC 
CCGAGGGTTC CACAAGCCAG CCCCAGGCCC TACTCTTCTG GCCCGGTGGG CTCAGAGAAG 
CCCCCCAAGG GCTCCAGCTA TAACCCGCCT CTGCCACCCC TGAAGATATC TACCTCTAAT 
GGCAGTCCAG GGTTCGACTA CCATCAGCCT GGGGACAAGT TTGACGCTAG CAAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317074.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172563.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X27, mRNA.

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CloneID	OMu79372
Clone ID Related Accession (Same CDS sequence)	XM_017317074.2 , XM_017317074.1
Accession Version	XM_017317074.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317074.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGACTCTGG AAGGAGGCTC TTACCGGGGG AGCTTGAAGG ACCCTACAGG CTGCCTGAAT 
GAGGGGATGA CCCCTCCCAC ACCTCCTAGA AACCTGGAAG AAGAACAGAA AGCCAAGGCG 
CTGAGAGGCA GGATGTTTGT CCTGAATGAG CTGGTACAGA CAGAGAAGGA CTACGTCAAG 
GACCTGGGGA TTGTGGTGGA GGGCTTCATG AAGAGAATAG AAGAGAAGGG GGTCCCTGAG 
GACATGCGAG GGAAGGAAAA GATCGTGTTT GGAAATATCC ACCAGATTTA CGACTGGCAT 
AAAGATTTTT TCCTGGCTGA ACTGGAAAAA TGTATCCAAG AGCAAGACAG ATTGGCCCAG 
CTCTTCATTA AACACGAGCG GAAGCTGCAC ATCTACGTGT GGTACTGCCA GAACAAGCCG 
CGTTCCGAGT ACATCGTGGC TGAGTACGAT GCCTATTTTG AGGAGGTTAA ACAAGAGATC 
AATCAAAGGC TGACCCTTAG TGACTTCCTG ATCAAGCCCA TTCAGAGAAT AACAAAGTAC 
CAGCTGCTAC TCAAGGACTT CCTGCGATAC AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT 
ATTGAGAAAG CAGTGGAGTT AATGTGCCTT GTCCCCAAGC GCTGTAATGA CATGATGAAT 
CTGGGACGCC TGCAGGGCTT CGAGGGCACC CTGACCGCCC AGGGCAAGCT ACTGCAGCAG 
GACACATTCT ATGTGATCGA GCTGGATGCC GGCATGCAGT CCCGGACCAA GGAGAGGCGT 
GTGTTTCTCT TTGAGCAGAT TGTCATCTTC AGTGAACTGC TCCGAAAGGG GTCCCTCACC 
CCAGGCTACA TGTTCAAAAG AAGCATCAAG ATGAATTACT TGGTCCTGGA AGACAATGTG 
GATGGTGACC CCTGCAAATT TGCACTCATG AACAGGGAGA CTTCAGAAAG GGTCATTCTA 
CAGGCTGCCA ACTCAGACAT CCAGCAGGCC TGGGTGCAGG ACATCAACCA AGTCCTAGAA 
ACACAGAGGG ACTTCTTAAA TGCACTACAG TCACCCATTG AGTATCAGCG GAAGGAGAGA 
AGCACAGCTG TGATCCGGTC CCAGCCCCCG AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC 
TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC CCCAAGGGCT CCAGCTATAA CCCGCCTCTG 
CCACCCCTGA AGATATCTAC CTCTAATGGC AGTCCAGGGT TCGACTACCA TCAGCCTGGG 
GACAAGTTTG ACGCTAGCAA GAACGACCTG GGAGGCTGCA ATGGGACATC AACCATGACT 
GTAATCAAAG ATTACTATGC ACTGAAGGAG AATGAAATCT GCGTGAGCCA AGGTGAGGTG 
GTACAGGTCC TCGCCGTCAA CCAGCAGAAC ATGTGTCTGG TGTACCAGCC TGCCAGCGAC 
CATTCCCCGG CAGCAGAGGG CTGGGTCCCA GGCAGCATCC TGGCGCCCCT CGCCAAAGCC 
ACAGCAGCGG CCGAAAGTAG TGACGGGAGC ATCAAAAAAG AACCCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172563.1
CDS	24875..32005
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKEPY

Target ORF information:

RefSeq Version	XM_017317074.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317074.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGACTCTGG AAGGAGGCTC TTACCGGGGG AGCTTGAAGG ACCCTACAGG CTGCCTGAAT 
GAGGGGATGA CCCCTCCCAC ACCTCCTAGA AACCTGGAAG AAGAACAGAA AGCCAAGGCG 
CTGAGAGGCA GGATGTTTGT CCTGAATGAG CTGGTACAGA CAGAGAAGGA CTACGTCAAG 
GACCTGGGGA TTGTGGTGGA GGGCTTCATG AAGAGAATAG AAGAGAAGGG GGTCCCTGAG 
GACATGCGAG GGAAGGAAAA GATCGTGTTT GGAAATATCC ACCAGATTTA CGACTGGCAT 
AAAGATTTTT TCCTGGCTGA ACTGGAAAAA TGTATCCAAG AGCAAGACAG ATTGGCCCAG 
CTCTTCATTA AACACGAGCG GAAGCTGCAC ATCTACGTGT GGTACTGCCA GAACAAGCCG 
CGTTCCGAGT ACATCGTGGC TGAGTACGAT GCCTATTTTG AGGAGGTTAA ACAAGAGATC 
AATCAAAGGC TGACCCTTAG TGACTTCCTG ATCAAGCCCA TTCAGAGAAT AACAAAGTAC 
CAGCTGCTAC TCAAGGACTT CCTGCGATAC AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT 
ATTGAGAAAG CAGTGGAGTT AATGTGCCTT GTCCCCAAGC GCTGTAATGA CATGATGAAT 
CTGGGACGCC TGCAGGGCTT CGAGGGCACC CTGACCGCCC AGGGCAAGCT ACTGCAGCAG 
GACACATTCT ATGTGATCGA GCTGGATGCC GGCATGCAGT CCCGGACCAA GGAGAGGCGT 
GTGTTTCTCT TTGAGCAGAT TGTCATCTTC AGTGAACTGC TCCGAAAGGG GTCCCTCACC 
CCAGGCTACA TGTTCAAAAG AAGCATCAAG ATGAATTACT TGGTCCTGGA AGACAATGTG 
GATGGTGACC CCTGCAAATT TGCACTCATG AACAGGGAGA CTTCAGAAAG GGTCATTCTA 
CAGGCTGCCA ACTCAGACAT CCAGCAGGCC TGGGTGCAGG ACATCAACCA AGTCCTAGAA 
ACACAGAGGG ACTTCTTAAA TGCACTACAG TCACCCATTG AGTATCAGCG GAAGGAGAGA 
AGCACAGCTG TGATCCGGTC CCAGCCCCCG AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC 
TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC CCCAAGGGCT CCAGCTATAA CCCGCCTCTG 
CCACCCCTGA AGATATCTAC CTCTAATGGC AGTCCAGGGT TCGACTACCA TCAGCCTGGG 
GACAAGTTTG ACGCTAGCAA GAACGACCTG GGAGGCTGCA ATGGGACATC AACCATGACT 
GTAATCAAAG ATTACTATGC ACTGAAGGAG AATGAAATCT GCGTGAGCCA AGGTGAGGTG 
GTACAGGTCC TCGCCGTCAA CCAGCAGAAC ATGTGTCTGG TGTACCAGCC TGCCAGCGAC 
CATTCCCCGG CAGCAGAGGG CTGGGTCCCA GGCAGCATCC TGGCGCCCCT CGCCAAAGCC 
ACAGCAGCGG CCGAAAGTAG TGACGGGAGC ATCAAAAAAG AACCCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317074.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172563.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X24, mRNA.

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CloneID	OMu79372
Clone ID Related Accession (Same CDS sequence)	XM_017317074.2 , XM_017317074.1
Accession Version	XM_017317074.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGACTCTGG AAGGAGGCTC TTACCGGGGG AGCTTGAAGG ACCCTACAGG CTGCCTGAAT 
GAGGGGATGA CCCCTCCCAC ACCTCCTAGA AACCTGGAAG AAGAACAGAA AGCCAAGGCG 
CTGAGAGGCA GGATGTTTGT CCTGAATGAG CTGGTACAGA CAGAGAAGGA CTACGTCAAG 
GACCTGGGGA TTGTGGTGGA GGGCTTCATG AAGAGAATAG AAGAGAAGGG GGTCCCTGAG 
GACATGCGAG GGAAGGAAAA GATCGTGTTT GGAAATATCC ACCAGATTTA CGACTGGCAT 
AAAGATTTTT TCCTGGCTGA ACTGGAAAAA TGTATCCAAG AGCAAGACAG ATTGGCCCAG 
CTCTTCATTA AACACGAGCG GAAGCTGCAC ATCTACGTGT GGTACTGCCA GAACAAGCCG 
CGTTCCGAGT ACATCGTGGC TGAGTACGAT GCCTATTTTG AGGAGGTTAA ACAAGAGATC 
AATCAAAGGC TGACCCTTAG TGACTTCCTG ATCAAGCCCA TTCAGAGAAT AACAAAGTAC 
CAGCTGCTAC TCAAGGACTT CCTGCGATAC AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT 
ATTGAGAAAG CAGTGGAGTT AATGTGCCTT GTCCCCAAGC GCTGTAATGA CATGATGAAT 
CTGGGACGCC TGCAGGGCTT CGAGGGCACC CTGACCGCCC AGGGCAAGCT ACTGCAGCAG 
GACACATTCT ATGTGATCGA GCTGGATGCC GGCATGCAGT CCCGGACCAA GGAGAGGCGT 
GTGTTTCTCT TTGAGCAGAT TGTCATCTTC AGTGAACTGC TCCGAAAGGG GTCCCTCACC 
CCAGGCTACA TGTTCAAAAG AAGCATCAAG ATGAATTACT TGGTCCTGGA AGACAATGTG 
GATGGTGACC CCTGCAAATT TGCACTCATG AACAGGGAGA CTTCAGAAAG GGTCATTCTA 
CAGGCTGCCA ACTCAGACAT CCAGCAGGCC TGGGTGCAGG ACATCAACCA AGTCCTAGAA 
ACACAGAGGG ACTTCTTAAA TGCACTACAG TCACCCATTG AGTATCAGCG GAAGGAGAGA 
AGCACAGCTG TGATCCGGTC CCAGCCCCCG AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC 
TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC CCCAAGGGCT CCAGCTATAA CCCGCCTCTG 
CCACCCCTGA AGATATCTAC CTCTAATGGC AGTCCAGGGT TCGACTACCA TCAGCCTGGG 
GACAAGTTTG ACGCTAGCAA GAACGACCTG GGAGGCTGCA ATGGGACATC AACCATGACT 
GTAATCAAAG ATTACTATGC ACTGAAGGAG AATGAAATCT GCGTGAGCCA AGGTGAGGTG 
GTACAGGTCC TCGCCGTCAA CCAGCAGAAC ATGTGTCTGG TGTACCAGCC TGCCAGCGAC 
CATTCCCCGG CAGCAGAGGG CTGGGTCCCA GGCAGCATCC TGGCGCCCCT CGCCAAAGCC 
ACAGCAGCGG CCGAAAGTAG TGACGGGAGC ATCAAAAAAG AACCCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172563.1
CDS	4891..12021
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKEPY

Target ORF information:

RefSeq Version	XM_017317074.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317074.1

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGACTCTGG AAGGAGGCTC TTACCGGGGG AGCTTGAAGG ACCCTACAGG CTGCCTGAAT 
GAGGGGATGA CCCCTCCCAC ACCTCCTAGA AACCTGGAAG AAGAACAGAA AGCCAAGGCG 
CTGAGAGGCA GGATGTTTGT CCTGAATGAG CTGGTACAGA CAGAGAAGGA CTACGTCAAG 
GACCTGGGGA TTGTGGTGGA GGGCTTCATG AAGAGAATAG AAGAGAAGGG GGTCCCTGAG 
GACATGCGAG GGAAGGAAAA GATCGTGTTT GGAAATATCC ACCAGATTTA CGACTGGCAT 
AAAGATTTTT TCCTGGCTGA ACTGGAAAAA TGTATCCAAG AGCAAGACAG ATTGGCCCAG 
CTCTTCATTA AACACGAGCG GAAGCTGCAC ATCTACGTGT GGTACTGCCA GAACAAGCCG 
CGTTCCGAGT ACATCGTGGC TGAGTACGAT GCCTATTTTG AGGAGGTTAA ACAAGAGATC 
AATCAAAGGC TGACCCTTAG TGACTTCCTG ATCAAGCCCA TTCAGAGAAT AACAAAGTAC 
CAGCTGCTAC TCAAGGACTT CCTGCGATAC AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT 
ATTGAGAAAG CAGTGGAGTT AATGTGCCTT GTCCCCAAGC GCTGTAATGA CATGATGAAT 
CTGGGACGCC TGCAGGGCTT CGAGGGCACC CTGACCGCCC AGGGCAAGCT ACTGCAGCAG 
GACACATTCT ATGTGATCGA GCTGGATGCC GGCATGCAGT CCCGGACCAA GGAGAGGCGT 
GTGTTTCTCT TTGAGCAGAT TGTCATCTTC AGTGAACTGC TCCGAAAGGG GTCCCTCACC 
CCAGGCTACA TGTTCAAAAG AAGCATCAAG ATGAATTACT TGGTCCTGGA AGACAATGTG 
GATGGTGACC CCTGCAAATT TGCACTCATG AACAGGGAGA CTTCAGAAAG GGTCATTCTA 
CAGGCTGCCA ACTCAGACAT CCAGCAGGCC TGGGTGCAGG ACATCAACCA AGTCCTAGAA 
ACACAGAGGG ACTTCTTAAA TGCACTACAG TCACCCATTG AGTATCAGCG GAAGGAGAGA 
AGCACAGCTG TGATCCGGTC CCAGCCCCCG AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC 
TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC CCCAAGGGCT CCAGCTATAA CCCGCCTCTG 
CCACCCCTGA AGATATCTAC CTCTAATGGC AGTCCAGGGT TCGACTACCA TCAGCCTGGG 
GACAAGTTTG ACGCTAGCAA GAACGACCTG GGAGGCTGCA ATGGGACATC AACCATGACT 
GTAATCAAAG ATTACTATGC ACTGAAGGAG AATGAAATCT GCGTGAGCCA AGGTGAGGTG 
GTACAGGTCC TCGCCGTCAA CCAGCAGAAC ATGTGTCTGG TGTACCAGCC TGCCAGCGAC 
CATTCCCCGG CAGCAGAGGG CTGGGTCCCA GGCAGCATCC TGGCGCCCCT CGCCAAAGCC 
ACAGCAGCGG CCGAAAGTAG TGACGGGAGC ATCAAAAAAG AACCCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_030249190.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105050.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X23, mRNA.

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CloneID	OMu86978
Clone ID Related Accession (Same CDS sequence)	XM_030249190.1
Accession Version	XM_030249190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGTCGTCTT TGCTAGCAGC CCGGCAGGCT CCCCCTGACG TGCCTACTGC TGCAGACCTT 
GTCAGTGCAA TAGAAAAGTT GGTCAAAAAT AAGCTGACTC TGGAAGGAGG CTCTTACCGG 
GGGAGCTTGA AGGACCCTAC AGGCTGCCTG AATGAGGGGA TGACCCCTCC CACACCTCCT 
AGAAACCTGG AAGAAGAACA GAAAGCCAAG GCGCTGAGAG GCAGGATGTT TGTCCTGAAT 
GAGCTGGTAC AGACAGAGAA GGACTACGTC AAGGACCTGG GGATTGTGGT GGAGGGCTTC 
ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT GAGGACATGC GAGGGAAGGA AAAGATCGTG 
TTTGGAAATA TCCACCAGAT TTACGACTGG CATAAAGATT TTTTCCTGGC TGAACTGGAA 
AAATGTATCC AAGAGCAAGA CAGATTGGCC CAGCTCTTCA TTAAACACGA GCGGAAGCTG 
CACATCTACG TGTGGTACTG CCAGAACAAG CCGCGTTCCG AGTACATCGT GGCTGAGTAC 
GATGCCTATT TTGAGGAGGT TAAACAAGAG ATCAATCAAA GGCTGACCCT TAGTGACTTC 
CTGATCAAGC CCATTCAGAG AATAACAAAG TACCAGCTGC TACTCAAGGA CTTCCTGCGA 
TACAGCGAGA AGGCTGGTTT GGAGTGTTCC GATATTGAGA AAGCAGTGGA GTTAATGTGC 
CTTGTCCCCA AGCGCTGTAA TGACATGATG AATCTGGGAC GCCTGCAGGG CTTCGAGGGC 
ACCCTGACCG CCCAGGGCAA GCTACTGCAG CAGGACACAT TCTATGTGAT CGAGCTGGAT 
GCCGGCATGC AGTCCCGGAC CAAGGAGAGG CGTGTGTTTC TCTTTGAGCA GATTGTCATC 
TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC ACCCCAGGCT ACATGTTCAA AAGAAGCATC 
AAGATGAATT ACTTGGTCCT GGAAGACAAT GTGGATGGTG ACCCCTGCAA ATTTGCACTC 
ATGAACAGGG AGACTTCAGA AAGGGTCATT CTACAGGCTG CCAACTCAGA CATCCAGCAG 
GCCTGGGTGC AGGACATCAA CCAAGTCCTA GAAACACAGA GGGACTTCTT AAATGCACTA 
CAGTCACCCA TTGAGTATCA GCGGAAGGAG AGAAGCACAG CTGTGATCCG GTCCCAGCCC 
CCGAGGGTTC CACAAGCCAG CCCCAGGCCC TACTCTTCTG GCCCGGTGGG CTCAGAGAAG 
CCCCCCAAGG GCTCCAGCTA TAACCCGCCT CTGCCACCCC TGAAGATATC TACCTCTAAT 
GGCAGTCCAG GGTTCGACTA CCATCAGCCT GGGGACAAGT TTGACGCTAG CAAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAAA AAGAACCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105050.1
CDS	24875..32098
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKEPY

Target ORF information:

RefSeq Version	XM_030249190.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249190.1

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGTCGTCTT TGCTAGCAGC CCGGCAGGCT CCCCCTGACG TGCCTACTGC TGCAGACCTT 
GTCAGTGCAA TAGAAAAGTT GGTCAAAAAT AAGCTGACTC TGGAAGGAGG CTCTTACCGG 
GGGAGCTTGA AGGACCCTAC AGGCTGCCTG AATGAGGGGA TGACCCCTCC CACACCTCCT 
AGAAACCTGG AAGAAGAACA GAAAGCCAAG GCGCTGAGAG GCAGGATGTT TGTCCTGAAT 
GAGCTGGTAC AGACAGAGAA GGACTACGTC AAGGACCTGG GGATTGTGGT GGAGGGCTTC 
ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT GAGGACATGC GAGGGAAGGA AAAGATCGTG 
TTTGGAAATA TCCACCAGAT TTACGACTGG CATAAAGATT TTTTCCTGGC TGAACTGGAA 
AAATGTATCC AAGAGCAAGA CAGATTGGCC CAGCTCTTCA TTAAACACGA GCGGAAGCTG 
CACATCTACG TGTGGTACTG CCAGAACAAG CCGCGTTCCG AGTACATCGT GGCTGAGTAC 
GATGCCTATT TTGAGGAGGT TAAACAAGAG ATCAATCAAA GGCTGACCCT TAGTGACTTC 
CTGATCAAGC CCATTCAGAG AATAACAAAG TACCAGCTGC TACTCAAGGA CTTCCTGCGA 
TACAGCGAGA AGGCTGGTTT GGAGTGTTCC GATATTGAGA AAGCAGTGGA GTTAATGTGC 
CTTGTCCCCA AGCGCTGTAA TGACATGATG AATCTGGGAC GCCTGCAGGG CTTCGAGGGC 
ACCCTGACCG CCCAGGGCAA GCTACTGCAG CAGGACACAT TCTATGTGAT CGAGCTGGAT 
GCCGGCATGC AGTCCCGGAC CAAGGAGAGG CGTGTGTTTC TCTTTGAGCA GATTGTCATC 
TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC ACCCCAGGCT ACATGTTCAA AAGAAGCATC 
AAGATGAATT ACTTGGTCCT GGAAGACAAT GTGGATGGTG ACCCCTGCAA ATTTGCACTC 
ATGAACAGGG AGACTTCAGA AAGGGTCATT CTACAGGCTG CCAACTCAGA CATCCAGCAG 
GCCTGGGTGC AGGACATCAA CCAAGTCCTA GAAACACAGA GGGACTTCTT AAATGCACTA 
CAGTCACCCA TTGAGTATCA GCGGAAGGAG AGAAGCACAG CTGTGATCCG GTCCCAGCCC 
CCGAGGGTTC CACAAGCCAG CCCCAGGCCC TACTCTTCTG GCCCGGTGGG CTCAGAGAAG 
CCCCCCAAGG GCTCCAGCTA TAACCCGCCT CTGCCACCCC TGAAGATATC TACCTCTAAT 
GGCAGTCCAG GGTTCGACTA CCATCAGCCT GGGGACAAGT TTGACGCTAG CAAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAAA AAGAACCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_030249191.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105051.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X25, mRNA.

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CloneID	OMu86979
Clone ID Related Accession (Same CDS sequence)	XM_030249191.1
Accession Version	XM_030249191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGACTCTGG AAGGAGGCTC TTACCGGGGG AGCTTGAAGG ACCCTACAGG CTGCCTGAAT 
GAGGGGATGA CCCCTCCCAC ACCTCCTAGA AACCTGGAAG AAGAACAGAA AGCCAAGGCG 
CTGAGAGGCA GGATGTTTGT CCTGAATGAG CTGGTACAGA CAGAGAAGGA CTACGTCAAG 
GACCTGGGGA TTGTGGTGGA GGGCTTCATG AAGAGAATAG AAGAGAAGGG GGTCCCTGAG 
GACATGCGAG GGAAGGAAAA GATCGTGTTT GGAAATATCC ACCAGATTTA CGACTGGCAT 
AAAGATTTTT TCCTGGCTGA ACTGGAAAAA TGTATCCAAG AGCAAGACAG ATTGGCCCAG 
CTCTTCATTA AACACGAGCG GAAGCTGCAC ATCTACGTGT GGTACTGCCA GAACAAGCCG 
CGTTCCGAGT ACATCGTGGC TGAGTACGAT GCCTATTTTG AGGAGGTTAA ACAAGAGATC 
AATCAAAGGC TGACCCTTAG TGACTTCCTG ATCAAGCCCA TTCAGAGAAT AACAAAGTAC 
CAGCTGCTAC TCAAGGACTT CCTGCGATAC AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT 
ATTGAGAAAG CAGTGGAGTT AATGTGCCTT GTCCCCAAGC GCTGTAATGA CATGATGAAT 
CTGGGACGCC TGCAGGGCTT CGAGGGCACC CTGACCGCCC AGGGCAAGCT ACTGCAGCAG 
GACACATTCT ATGTGATCGA GCTGGATGCC GGCATGCAGT CCCGGACCAA GGAGAGGCGT 
GTGTTTCTCT TTGAGCAGAT TGTCATCTTC AGTGAACTGC TCCGAAAGGG GTCCCTCACC 
CCAGGCTACA TGTTCAAAAG AAGCATCAAG ATGAATTACT TGGTCCTGGA AGACAATGTG 
GATGGTGACC CCTGCAAATT TGCACTCATG AACAGGGAGA CTTCAGAAAG GGTCATTCTA 
CAGGCTGCCA ACTCAGACAT CCAGCAGGCC TGGGTGCAGG ACATCAACCA AGTCCTAGAA 
ACACAGAGGG ACTTCTTAAA TGCACTACAG TCACCCATTG AGTATCAGCG GAAGGAGAGA 
AGCACAGCTG TGATCCGGTC CCAGCCCCCG AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC 
TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC CCCAAGGGCT CCAGCTATAA CCCGCCTCTG 
CCACCCCTGA AGATATCTAC CTCTAATGGC AGTCCAGGGT TCGACTACCA TCAGCCTGGG 
GACAAGTTTG ACGCTAGCAA GCAGAACGAC CTGGGAGGCT GCAATGGGAC ATCAACCATG 
ACTGTAATCA AAGATTACTA TGCACTGAAG GAGAATGAAA TCTGCGTGAG CCAAGGTGAG 
GTGGTACAGG TCCTCGCCGT CAACCAGCAG AACATGTGTC TGGTGTACCA GCCTGCCAGC 
GACCATTCCC CGGCAGCAGA GGGCTGGGTC CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA 
GCCACAGCAG CGGCCGAAAG TAGTGACGGG AGCATCAAAA AAGAACCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105051.1
CDS	24875..32008
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKEPY

Target ORF information:

RefSeq Version	XM_030249191.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249191.1

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGACTCTGG AAGGAGGCTC TTACCGGGGG AGCTTGAAGG ACCCTACAGG CTGCCTGAAT 
GAGGGGATGA CCCCTCCCAC ACCTCCTAGA AACCTGGAAG AAGAACAGAA AGCCAAGGCG 
CTGAGAGGCA GGATGTTTGT CCTGAATGAG CTGGTACAGA CAGAGAAGGA CTACGTCAAG 
GACCTGGGGA TTGTGGTGGA GGGCTTCATG AAGAGAATAG AAGAGAAGGG GGTCCCTGAG 
GACATGCGAG GGAAGGAAAA GATCGTGTTT GGAAATATCC ACCAGATTTA CGACTGGCAT 
AAAGATTTTT TCCTGGCTGA ACTGGAAAAA TGTATCCAAG AGCAAGACAG ATTGGCCCAG 
CTCTTCATTA AACACGAGCG GAAGCTGCAC ATCTACGTGT GGTACTGCCA GAACAAGCCG 
CGTTCCGAGT ACATCGTGGC TGAGTACGAT GCCTATTTTG AGGAGGTTAA ACAAGAGATC 
AATCAAAGGC TGACCCTTAG TGACTTCCTG ATCAAGCCCA TTCAGAGAAT AACAAAGTAC 
CAGCTGCTAC TCAAGGACTT CCTGCGATAC AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT 
ATTGAGAAAG CAGTGGAGTT AATGTGCCTT GTCCCCAAGC GCTGTAATGA CATGATGAAT 
CTGGGACGCC TGCAGGGCTT CGAGGGCACC CTGACCGCCC AGGGCAAGCT ACTGCAGCAG 
GACACATTCT ATGTGATCGA GCTGGATGCC GGCATGCAGT CCCGGACCAA GGAGAGGCGT 
GTGTTTCTCT TTGAGCAGAT TGTCATCTTC AGTGAACTGC TCCGAAAGGG GTCCCTCACC 
CCAGGCTACA TGTTCAAAAG AAGCATCAAG ATGAATTACT TGGTCCTGGA AGACAATGTG 
GATGGTGACC CCTGCAAATT TGCACTCATG AACAGGGAGA CTTCAGAAAG GGTCATTCTA 
CAGGCTGCCA ACTCAGACAT CCAGCAGGCC TGGGTGCAGG ACATCAACCA AGTCCTAGAA 
ACACAGAGGG ACTTCTTAAA TGCACTACAG TCACCCATTG AGTATCAGCG GAAGGAGAGA 
AGCACAGCTG TGATCCGGTC CCAGCCCCCG AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC 
TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC CCCAAGGGCT CCAGCTATAA CCCGCCTCTG 
CCACCCCTGA AGATATCTAC CTCTAATGGC AGTCCAGGGT TCGACTACCA TCAGCCTGGG 
GACAAGTTTG ACGCTAGCAA GCAGAACGAC CTGGGAGGCT GCAATGGGAC ATCAACCATG 
ACTGTAATCA AAGATTACTA TGCACTGAAG GAGAATGAAA TCTGCGTGAG CCAAGGTGAG 
GTGGTACAGG TCCTCGCCGT CAACCAGCAG AACATGTGTC TGGTGTACCA GCCTGCCAGC 
GACCATTCCC CGGCAGCAGA GGGCTGGGTC CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA 
GCCACAGCAG CGGCCGAAAG TAGTGACGGG AGCATCAAAA AAGAACCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_030249192.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105052.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X26, mRNA.

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CloneID	OMu86980
Clone ID Related Accession (Same CDS sequence)	XM_030249192.1
Accession Version	XM_030249192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGACTC TGGAAGGAGG CTCTTACCGG GGGAGCTTGA AGGACCCTAC AGGCTGCCTG 
AATGAGGGGA TGACCCCTCC CACACCTCCT AGAAACCTGG AAGAAGAACA GAAAGCCAAG 
GCGCTGAGAG GCAGGATGTT TGTCCTGAAT GAGCTGGTAC AGACAGAGAA GGACTACGTC 
AAGGACCTGG GGATTGTGGT GGAGGGCTTC ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT 
GAGGACATGC GAGGGAAGGA AAAGATCGTG TTTGGAAATA TCCACCAGAT TTACGACTGG 
CATAAAGATT TTTTCCTGGC TGAACTGGAA AAATGTATCC AAGAGCAAGA CAGATTGGCC 
CAGCTCTTCA TTAAACACGA GCGGAAGCTG CACATCTACG TGTGGTACTG CCAGAACAAG 
CCGCGTTCCG AGTACATCGT GGCTGAGTAC GATGCCTATT TTGAGGAGGT TAAACAAGAG 
ATCAATCAAA GGCTGACCCT TAGTGACTTC CTGATCAAGC CCATTCAGAG AATAACAAAG 
TACCAGCTGC TACTCAAGGA CTTCCTGCGA TACAGCGAGA AGGCTGGTTT GGAGTGTTCC 
GATATTGAGA AAGCAGTGGA GTTAATGTGC CTTGTCCCCA AGCGCTGTAA TGACATGATG 
AATCTGGGAC GCCTGCAGGG CTTCGAGGGC ACCCTGACCG CCCAGGGCAA GCTACTGCAG 
CAGGACACAT TCTATGTGAT CGAGCTGGAT GCCGGCATGC AGTCCCGGAC CAAGGAGAGG 
CGTGTGTTTC TCTTTGAGCA GATTGTCATC TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC 
ACCCCAGGCT ACATGTTCAA AAGAAGCATC AAGATGAATT ACTTGGTCCT GGAAGACAAT 
GTGGATGGTG ACCCCTGCAA ATTTGCACTC ATGAACAGGG AGACTTCAGA AAGGGTCATT 
CTACAGGCTG CCAACTCAGA CATCCAGCAG GCCTGGGTGC AGGACATCAA CCAAGTCCTA 
GAAACACAGA GGGACTTCTT AAATGCACTA CAGTCACCCA TTGAGTATCA GCGGAAGGAG 
AGAAGCACAG CTGTGATCCG GTCCCAGCCC CCGAGGGTTC CACAAGCCAG CCCCAGGCCC 
TACTCTTCTG GCCCGGTGGG CTCAGAGAAG CCCCCCAAGG GCTCCAGCTA TAACCCGCCT 
CTGCCACCCC TGAAGATATC TACCTCTAAT GGCAGTCCAG GGTTCGACTA CCATCAGCCT 
GGGGACAAGT TTGACGCTAG CAAGAACGAC CTGGGAGGCT GCAATGGGAC ATCAACCATG 
ACTGTAATCA AAGATTACTA TGCACTGAAG GAGAATGAAA TCTGCGTGAG CCAAGGTGAG 
GTGGTACAGG TCCTCGCCGT CAACCAGCAG AACATGTGTC TGGTGTACCA GCCTGCCAGC 
GACCATTCCC CGGCAGCAGA GGGCTGGGTC CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA 
GCCACAGCAG CGGCCGAAAG TAGTGACGGG AGCATCAAAA AAGAACCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105052.1
CDS	24875..32008
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKEPY

Target ORF information:

RefSeq Version	XM_030249192.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249192.1

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGACTC TGGAAGGAGG CTCTTACCGG GGGAGCTTGA AGGACCCTAC AGGCTGCCTG 
AATGAGGGGA TGACCCCTCC CACACCTCCT AGAAACCTGG AAGAAGAACA GAAAGCCAAG 
GCGCTGAGAG GCAGGATGTT TGTCCTGAAT GAGCTGGTAC AGACAGAGAA GGACTACGTC 
AAGGACCTGG GGATTGTGGT GGAGGGCTTC ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT 
GAGGACATGC GAGGGAAGGA AAAGATCGTG TTTGGAAATA TCCACCAGAT TTACGACTGG 
CATAAAGATT TTTTCCTGGC TGAACTGGAA AAATGTATCC AAGAGCAAGA CAGATTGGCC 
CAGCTCTTCA TTAAACACGA GCGGAAGCTG CACATCTACG TGTGGTACTG CCAGAACAAG 
CCGCGTTCCG AGTACATCGT GGCTGAGTAC GATGCCTATT TTGAGGAGGT TAAACAAGAG 
ATCAATCAAA GGCTGACCCT TAGTGACTTC CTGATCAAGC CCATTCAGAG AATAACAAAG 
TACCAGCTGC TACTCAAGGA CTTCCTGCGA TACAGCGAGA AGGCTGGTTT GGAGTGTTCC 
GATATTGAGA AAGCAGTGGA GTTAATGTGC CTTGTCCCCA AGCGCTGTAA TGACATGATG 
AATCTGGGAC GCCTGCAGGG CTTCGAGGGC ACCCTGACCG CCCAGGGCAA GCTACTGCAG 
CAGGACACAT TCTATGTGAT CGAGCTGGAT GCCGGCATGC AGTCCCGGAC CAAGGAGAGG 
CGTGTGTTTC TCTTTGAGCA GATTGTCATC TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC 
ACCCCAGGCT ACATGTTCAA AAGAAGCATC AAGATGAATT ACTTGGTCCT GGAAGACAAT 
GTGGATGGTG ACCCCTGCAA ATTTGCACTC ATGAACAGGG AGACTTCAGA AAGGGTCATT 
CTACAGGCTG CCAACTCAGA CATCCAGCAG GCCTGGGTGC AGGACATCAA CCAAGTCCTA 
GAAACACAGA GGGACTTCTT AAATGCACTA CAGTCACCCA TTGAGTATCA GCGGAAGGAG 
AGAAGCACAG CTGTGATCCG GTCCCAGCCC CCGAGGGTTC CACAAGCCAG CCCCAGGCCC 
TACTCTTCTG GCCCGGTGGG CTCAGAGAAG CCCCCCAAGG GCTCCAGCTA TAACCCGCCT 
CTGCCACCCC TGAAGATATC TACCTCTAAT GGCAGTCCAG GGTTCGACTA CCATCAGCCT 
GGGGACAAGT TTGACGCTAG CAAGAACGAC CTGGGAGGCT GCAATGGGAC ATCAACCATG 
ACTGTAATCA AAGATTACTA TGCACTGAAG GAGAATGAAA TCTGCGTGAG CCAAGGTGAG 
GTGGTACAGG TCCTCGCCGT CAACCAGCAG AACATGTGTC TGGTGTACCA GCCTGCCAGC 
GACCATTCCC CGGCAGCAGA GGGCTGGGTC CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA 
GCCACAGCAG CGGCCGAAAG TAGTGACGGG AGCATCAAAA AAGAACCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522380.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522443.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X28, mRNA.

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CloneID	OMu52296
Clone ID Related Accession (Same CDS sequence)	XM_006522380.1
Accession Version	XM_006522380.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCT CTTTCCACAC GCACACCAAA GAGCTGTGGA CATGGATGGA GGACCTTCAG 
AAGGAGGTGC TGGAGGATGT CTGTGCAGAC TCTGTGGATG CGGTCCAGGA GCTGATCAAG 
CAGTTCCAGC AGCAGCAGAC CGCCACCCTG GATGCCACTC TCAATGTCAT CAAGGAAGGC 
GAAGACCTTA TCCAGCAGCT CAGGTCAGCG CCTCCCTCCC TGGGGGAGCC CACCGAGGCC 
AGGGACTCAG CGATGTCCAA CAACAAGACA CCCCACAGTA GCTCTATCAG CCATATTGAG 
TCTGTCCTCC AGCAGCTGGA CGATGCTCAG GTACAGATGG AAGAGCTGTT TCACGAGCGG 
AAGATCAAGC TGGACATCTT CCTACAGCTG CGCATCTTTG AACAGTACAC CATTGAGGTC 
ACTGCAGAGC TGGATGCCTG GAACGAGGAC TTGCTCCGGC AGATGAATGA TTTCAACACA 
GAGGATCTGA CCCTGGCTGA GCAGCGGCTA CAGCGCCACA CAGAACGGAA GCTTGCCATG 
AACAACATGA CGTTCGAGGT CATCCAGCAG GGCCAGGACC TGCACCAGTA CATCATGGAA 
GTGCAAGCGT CAGGAATCGA GTTAATCTGT GAGAAGGACC TTGACCTGGC AGCTCAGGTG 
CAGGAGCTGC TGGAGTTTCT CCATGAGAAG CAGCACGAGC TGGAGCTCAA TGCGGAGCAG 
ACGCACAAGC GGCTAGAGCA GTGTCTCCAG CTGAGGCACC TCCAGGCTGA GGTCAAACAG 
GTTCTGGGAT GGATCCGCAA TGGAGAGTCG ATGCTCAACG CCAGCCTGGT CAACGCCAGC 
TCTTTGTCGG AGGCAGAGCA GCTGCAGCGG GAGCATGAAC AGTTCCAGCT AGCCATCGAG 
TCCCTCTTTC ATGCCACTTC CTTGCAGAAG ACGCACCAGA GCGCCCTGCA GGTACAGCAG 
AAGGCAGAGG CACTGCTCCA GGCCGGCCAC TACGACGCAG ACGCCATCCG GGAATGTGCT 
GAGAAGGTGG CCCTCCACTG GCAACAGCTC ATGCTAAAGA TGGAGGACCG GCTGAAGCTT 
GTCAACGCCT CTGTGGCCTT TTACAAGACC TCTGAGCAGG TGTGCAGTGT TCTGGAGAGC 
TTGGAGCAGG AGTACCGGAG AGACGAGGAC TGGTGTGGTG GGAGAGACAA GCTGGGGCCG 
GCGGCAGAGA TGGACCACGT TATTCCACTC CTCAGCAAGC ACCTGGAGCA GAAGGAGGCC 
TTCCTGAAGG CCTGCACGCT GGCAAGGCGG AACGCCGAGG TGTTCCTCAA ATACATCCAC 
AGGAACAATG TGAGCATGCC TAGTGTTGCC AGCCACACCC GGGGGCCTGA ACAGCAAGTA 
AAAGCCATCT TGAGCGAGCT CTTGCAGAGG GAGAATCGAG TCCTGCACTT CTGGACCTTG 
AAGAAGCGGC GGTTAGACCA GTGTCAGCAA TATGTGGTGT TTGAGCGCAG TGCTAAGCAG 
GCACTGGACT GGATCCAAGA AACAGGAGAA TATTACCTCT CAACACATAC CTCCACCGGA 
GAGACCACGG AGGAGACTCA GGAGCTGCTG AAAGAGTATG GAGAATTCAG GGTGCCTGCC 
AAGCAAACGA AGGAGAAGGT GAAACTTCTG ATCCAGCTAG CAGACAGCTT TGTGGAAAAG 
GGCCACATCC ACGCCACAGA GATACGGAAA TGGGTGACCA CGGTGGACAA GCACTACCGC 
GATTTCTCCC TGAGGATGGG GAAGTACCGG TACTCGCTGG AGAAGGCGCT GGGGGTCAAC 
ACGGAGGATA ACAAGGACCT AGAGCTGGAC ATCATCCCAG CAAGCCTTTC GGATCGGGAG 
GTCAAGCTCC GGGACGCTAA CCATGAGATC AATGAAGAGA AACGGAAGTC AGCTCGAAAG 
AAAGAGTTCA TTATGGCTGA ACTGCTCCAA ACTGAGAAGG CTTATGTAAG GGACTTGCAC 
GAGTGCTTGG AGACCTACCT GTGGGAGATG ACTAGTGGTG TGGAAGAGAT ACCCCCCGGC 
ATCCTCAATA AGGAACATAT CATCTTCGGC AACATCCAGG AGATCTATGA CTTCCATAAC 
AACATCTTCC TCAAAGAGCT GGAGAAGTAC GAGCAGCTGC CTGAGGATGT GGGACACTGC 
TTTGTTACCT GGGCAGACAA ATTTCAGATG TATGTCACCT ACTGTAAAAA CAAGCCTGAT 
TCCAACCAGC TGATCCTGGA GCATGCGGGC ACCTTCTTTG ATGAGATCCA GCAGCGGCAT 
GGCCTGGCCA ACTCCATCTC CTCCTACCTA ATTAAGCCTG TCCAGAGGGT TACCAAGTAC 
CAGCTGCTCC TGAAGGAACT TCTAACTTGC TGTGAAGAAG GGAAAGGAGA GCTGAAGGAT 
GGCCTGGAGG TGATGCTCAG TGTCCCCAAG AAAGCCAACG ATGCCATGCA TGTCAGCATG 
CTGGAAGGGT TCGATGAAAA CTTGGATGTA CAGGGGGAGT TGATTCTCCA GGATGCCTTT 
CAAGTGTGGG ACCCAAAGTC GCTGATCCGG AAGGGGCGGG AGCGCCATCT GTTCCTCTTT 
GAGATCTCCT TGGTGTTTAG CAAAGAGATC AAAGACTCTT CAGGACACAC GAAATATGTT 
TACAAGAACA AGCTACTGAC CTCAGAGCTG GGTGTGACCG AGCATGTAGA AGGTGACCCC 
TGCAAATTCG CCTTGTGGTC TGGGCGCACC CCATCCTCAG ACAATAAAAC AGTGCTCAAA 
GCTTCCAACA TCGAAACCAA GCAGGAATGG ATCAAGAACA TTCGAGAAGT GATTCAGGAG 
AGGATCATTC ATCTTAAAGG CGCTTTAAAG GAGCCAATTC AGCTCCCCAA AACACCAGCC 
AAACTGAGGA ACAATAGTAA GAGGGATGGG GTAGAGGATG GTGACAGCCA AGGGGATGGG 
AGCAGCCAGC CAGATACCAT ATCCATTGCC TCCAGGACCT CTCAGAACAC AGTGGAAAGT 
GACAAGCTCT CAGGTGGATG TGAGCTGACC GTGGTCCTCC AGGACTTCAG CGCAGGCCAC 
AGCAGCGAGC TGAGCATCCA GGTGGGCCAG ACGGTGGAGC TGCTGGAACG ACCGAGTGAG 
AGGCCTGGCT GGTGTCTGGT CCGCACCACA GAGCGGAGCC CCCCACAGGA AGGCCTGGTA 
CCCAGCAGCG CCCTGTGCAT CTCACACTCT CGCAGCAGCG TGGAAATGGA CTGCTTCTTC 
CCCTTGAAAG ATTCATACTC TCATTCCTCC AGTGAGAATG GAGGCAAATC CGAGTCCGTG 
GCCCACCTGC AGTCTCAGCC CTCCCTGAAC TCCATCCACA GTTCCCCTGG TCCCAAGCGC 
TCCACCAACA CCCTCAAAAA GTGGCTGACC AGCCCCGTGC GCCGCCTCAA CAGCGGAAAA 
GCCGACGGGA ACATCAAAAA GCAGAAGAAA GTCCGGGATG GGCGGAAGAG CTTCGATCTG 
GGATCGCCCA AGCCTGGGGA TGAGACGACA CCTCAGGGAG ACAGCGCTGA CGAGAAGAGC 
AAGAAGGGTT GGGGCGAAGA CGAGCCAGAT GAAGAGTCAC ACACACCCCT CCCTCCGCCC 
ATGAAGATCT TTGACAACGA CCCCACACAG GATGAGATGT CGTCTTTGCT AGCAGCCCGG 
CAGGCTCCCC CTGACGTGCC TACTGCTGCA GACCTTGTCA GTGCAATAGA AAAGTTGGTC 
AAAAATAAGC TGACTCTGGA AGGAGGCTCT TACCGGGGGA GCTTGAAGGA CCCTACAGGC 
TGCCTGAATG AGGGGATGAC CCCTCCCACA CCTCCTAGAA ACCTGGAAGA AGAACAGAAA 
GCCAAGGCGC TGAGAGGCAG GATGTTTGTC CTGAATGAGC TGGTACAGAC AGAGAAGGAC 
TACGTCAAGG ACCTGGGGAT TGTGGTGGAG GGCTTCATGA AGAGAATAGA AGAGAAGGGG 
GTCCCTGAGG ACATGCGAGG GAAGGAAAAG ATCGTGTTTG GAAATATCCA CCAGATTTAC 
GACTGGCATA AAGATTTTTT CCTGGCTGAA CTGGAAAAAT GTATCCAAGA GCAAGACAGA 
TTGGCCCAGC TCTTCATTAA ACACGAGCGG AAGCTGCACA TCTACGTGTG GTACTGCCAG 
AACAAGCCGC GTTCCGAGTA CATCGTGGCT GAGTACGATG CCTATTTTGA GGAGGTTAAA 
CAAGAGATCA ATCAAAGGCT GACCCTTAGT GACTTCCTGA TCAAGCCCAT TCAGAGAATA 
ACAAAGTACC AGCTGCTACT CAAGGACTTC CTGCGATACA GCGAGAAGGC TGGTTTGGAG 
TGTTCCGATA TTGAGAAAGC AGTGGAGTTA ATGTGCCTTG TCCCCAAGCG CTGTAATGAC 
ATGATGAATC TGGGACGCCT GCAGGGCTTC GAGGGCACCC TGACCGCCCA GGGCAAGCTA 
CTGCAGCAGG ACACATTCTA TGTGATCGAG CTGGATGCCG GCATGCAGTC CCGGACCAAG 
GAGAGGCGTG TGTTTCTCTT TGAGCAGATT GTCATCTTCA GTGAACTGCT CCGAAAGGGG 
TCCCTCACCC CAGGCTACAT GTTCAAAAGA AGCATCAAGA TGAATTACTT GGTCCTGGAA 
GACAATGTGG ATGGTGACCC CTGCAAATTT GCACTCATGA ACAGGGAGAC TTCAGAAAGG 
GTCATTCTAC AGGCTGCCAA CTCAGACATC CAGCAGGCCT GGGTGCAGGA CATCAACCAA 
GTCCTAGAAA CACAGAGGGA CTTCTTAAAT GCACTACAGT CACCCATTGA GTATCAGCGG 
AAGGAGAGAA GCACAGCTGT GATCCGGTCC CAGCCCCCGA GGGTTCCACA AGCCAGCCCC 
AGGCCCTACT CTTCTGGCCC GGTGGGCTCA GAGAAGCCCC CCAAGGGCTC CAGCTATAAC 
CCGCCTCTGC CACCCCTGAA GATATCTACC TCTAATGGCA GTCCAGGGTT CGACTACCAT 
CAGCCTGGGG ACAAGTTTGA CGCTAGCAAG CAGAACGACC TGGGAGGCTG CAATGGGACA 
TCAACCATGA CTGTAATCAA AGATTACTAT GCACTGAAGG AGAATGAAAT CTGCGTGAGC 
CAAGGTGAGG TGGTACAGGT CCTCGCCGTC AACCAGCAGA ACATGTGTCT GGTGTACCAG 
CCTGCCAGCG ACCATTCCCC GGCAGCAGAG GGCTGGGTCC CAGGCAGCAT CCTGGCGCCC 
CTCGCCAAAG CCACAGCAGC GGCCGAAAGT AGTGACGGGA GCATCAAGAA GTCATGCTCT 
TGGCACACTC TACGCATGAG AAAGCGGGCA GACGTGGAGA ACTCGGGTAA AAATGAAGCC 
ACAGGGCCTC GTAAACCCAA GGATATTCTG GGCAACAAAG TCTCTGTTAA AGAGACGAAC 
AGTTCCGAGG AGTCAGAGTG TGATGATCTT GACCCTAATA CTAGTATGGA GATCTTGAAT 
CCAAATTTCA TCCAGGAAGT GGCTCCAGAA TTCCTCGTGC CCTTAGTAGA TGTGACCTGT 
TTGCTTGGAG ACACAGTGCT ACTGCAGTGT AAAGCCTGTG GGCGGCCAAA GCCCAGCATC 
ACCTGGAAGG GTCCGGATCA GAACATCCTA GACACTGACA ACAGTTCAGC AACATACACC 
ATCTCCTCCT GTGATTCTGG GGAAAGCACC CTCAAGATCT GCAACCTGAT GCCCCAGGAT 
AGTGGGATTT ATACCTGCAT AGCAGCAAAC GACCACGGGA CTGCATCCAC ATCTGCTACA 
GTTAAGGTGC AAGGTGTCCC CGCAGCCCCC AACCGCCCAA TCGCACAGGA GAGGAGCTGC 
ACCTCCGTCA TCCTCCGCTG GCTGCCCCCA GCCAGCACCG GAAACTGCAC TATTTCGGGG 
TACACAGTGG AGTATCGCGA GGAAGGTTCC CAGGTCTGGC AGCAATCCGT AGCTTCGACC 
TTGGACACTT ACCTGGTCAT TGAAGACCTT AGTCCTGGGT GCCCTTATCA GTTCAGGGTC 
AGTGCCAGCA ACCCCTGGGG AATCAGCCTT CCCAGTGAGC CCTCGGAGTT TGTGCGCCTT 
CCAGAGTACG ATGCAGCCGC CGACGGTGCC ACCATTTCTT GGAAGGAAAA TTTTGATTCT 
GCTTACACTG AACTGAACGA GATTGGCAGA GGCCGTTTCT CTATTGTAAA GAAATGCATT 
CACAAAGCTA CCCGCAAAGA TGTTGCTGTG AAATTTGTGA GCAAAAAAAT GAAGAAGAAG 
GAGCAGGCTG CTCACGAGGC CGCCCTGCTC CAGCACCTGC AGCACCCTCA GTATGTCACG 
CTCCATGACA CCTACGAGTC CCCCACATCC TACATCCTGA TTTTGGAACT GATGGATGAC 
GGCCGACTCT TGGACTACCT TATGAATCAT GATGAGCTGA TGGAAGAAAA GGTAGCTTTC 
TACATTCGAG ACATCATGGA AGCTCTGCAG TACCTGCACA ACTGTAGGGT CGCACATTTG 
GACATAAAGC CCGAAAACCT GCTCATTGAC CTCCGGATCC CAGTGCCTCG AGTGAAGCTC 
ATCGACTTGG AAGACGCTGT CCAGATCTCA GGCCACTTCC ATATCCACCA TCTGCTGGGG 
AACCCTGAGT TCGCTGCCCC CGAAGTGATC CAAGGCATCC CTGTCTCCCT GGGCACAGAC 
ATCTGGAGCA TCGGGGTTCT GACATATGTC ATGCTTAGTG GGGTCTCCCC CTTCTTAGAT 
GAGAGCAAAG AGGAAACCTG TATCAACGTG TGCAGGGTGG ACTTTAGCTT CCCCCACGAG 
TACTTCTGCG GTGTGAGCAA TGCTGCCAGG GATTTCATCA ACGTAATCTT ACAGGAAGAC 
TTCCGGAGGC GGCCCACAGC AGCCACGTGC CTGCAGCATC CCTGGCTACA GCCCCACAAC 
GGCAGCTACT CCAAGATCCC CTTGGACACC TCCCGTCTGG CCTGCTTCAT AGAACGCCGC 
AAGCACCAGA ATGATGTGCG GCCCATTCCC AATGTCAAGA GTTACATCGT CAATAGGGTG 
AACCAAGGGA CCTCGCTATC CCACAACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522443.1
CDS	310..7362
Translation	MSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_006522380.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522380.1

ORF Insert Sequence:

ATGTCGGTCT CTTTCCACAC GCACACCAAA GAGCTGTGGA CATGGATGGA GGACCTTCAG 
AAGGAGGTGC TGGAGGATGT CTGTGCAGAC TCTGTGGATG CGGTCCAGGA GCTGATCAAG 
CAGTTCCAGC AGCAGCAGAC CGCCACCCTG GATGCCACTC TCAATGTCAT CAAGGAAGGC 
GAAGACCTTA TCCAGCAGCT CAGGTCAGCG CCTCCCTCCC TGGGGGAGCC CACCGAGGCC 
AGGGACTCAG CGATGTCCAA CAACAAGACA CCCCACAGTA GCTCTATCAG CCATATTGAG 
TCTGTCCTCC AGCAGCTGGA CGATGCTCAG GTACAGATGG AAGAGCTGTT TCACGAGCGG 
AAGATCAAGC TGGACATCTT CCTACAGCTG CGCATCTTTG AACAGTACAC CATTGAGGTC 
ACTGCAGAGC TGGATGCCTG GAACGAGGAC TTGCTCCGGC AGATGAATGA TTTCAACACA 
GAGGATCTGA CCCTGGCTGA GCAGCGGCTA CAGCGCCACA CAGAACGGAA GCTTGCCATG 
AACAACATGA CGTTCGAGGT CATCCAGCAG GGCCAGGACC TGCACCAGTA CATCATGGAA 
GTGCAAGCGT CAGGAATCGA GTTAATCTGT GAGAAGGACC TTGACCTGGC AGCTCAGGTG 
CAGGAGCTGC TGGAGTTTCT CCATGAGAAG CAGCACGAGC TGGAGCTCAA TGCGGAGCAG 
ACGCACAAGC GGCTAGAGCA GTGTCTCCAG CTGAGGCACC TCCAGGCTGA GGTCAAACAG 
GTTCTGGGAT GGATCCGCAA TGGAGAGTCG ATGCTCAACG CCAGCCTGGT CAACGCCAGC 
TCTTTGTCGG AGGCAGAGCA GCTGCAGCGG GAGCATGAAC AGTTCCAGCT AGCCATCGAG 
TCCCTCTTTC ATGCCACTTC CTTGCAGAAG ACGCACCAGA GCGCCCTGCA GGTACAGCAG 
AAGGCAGAGG CACTGCTCCA GGCCGGCCAC TACGACGCAG ACGCCATCCG GGAATGTGCT 
GAGAAGGTGG CCCTCCACTG GCAACAGCTC ATGCTAAAGA TGGAGGACCG GCTGAAGCTT 
GTCAACGCCT CTGTGGCCTT TTACAAGACC TCTGAGCAGG TGTGCAGTGT TCTGGAGAGC 
TTGGAGCAGG AGTACCGGAG AGACGAGGAC TGGTGTGGTG GGAGAGACAA GCTGGGGCCG 
GCGGCAGAGA TGGACCACGT TATTCCACTC CTCAGCAAGC ACCTGGAGCA GAAGGAGGCC 
TTCCTGAAGG CCTGCACGCT GGCAAGGCGG AACGCCGAGG TGTTCCTCAA ATACATCCAC 
AGGAACAATG TGAGCATGCC TAGTGTTGCC AGCCACACCC GGGGGCCTGA ACAGCAAGTA 
AAAGCCATCT TGAGCGAGCT CTTGCAGAGG GAGAATCGAG TCCTGCACTT CTGGACCTTG 
AAGAAGCGGC GGTTAGACCA GTGTCAGCAA TATGTGGTGT TTGAGCGCAG TGCTAAGCAG 
GCACTGGACT GGATCCAAGA AACAGGAGAA TATTACCTCT CAACACATAC CTCCACCGGA 
GAGACCACGG AGGAGACTCA GGAGCTGCTG AAAGAGTATG GAGAATTCAG GGTGCCTGCC 
AAGCAAACGA AGGAGAAGGT GAAACTTCTG ATCCAGCTAG CAGACAGCTT TGTGGAAAAG 
GGCCACATCC ACGCCACAGA GATACGGAAA TGGGTGACCA CGGTGGACAA GCACTACCGC 
GATTTCTCCC TGAGGATGGG GAAGTACCGG TACTCGCTGG AGAAGGCGCT GGGGGTCAAC 
ACGGAGGATA ACAAGGACCT AGAGCTGGAC ATCATCCCAG CAAGCCTTTC GGATCGGGAG 
GTCAAGCTCC GGGACGCTAA CCATGAGATC AATGAAGAGA AACGGAAGTC AGCTCGAAAG 
AAAGAGTTCA TTATGGCTGA ACTGCTCCAA ACTGAGAAGG CTTATGTAAG GGACTTGCAC 
GAGTGCTTGG AGACCTACCT GTGGGAGATG ACTAGTGGTG TGGAAGAGAT ACCCCCCGGC 
ATCCTCAATA AGGAACATAT CATCTTCGGC AACATCCAGG AGATCTATGA CTTCCATAAC 
AACATCTTCC TCAAAGAGCT GGAGAAGTAC GAGCAGCTGC CTGAGGATGT GGGACACTGC 
TTTGTTACCT GGGCAGACAA ATTTCAGATG TATGTCACCT ACTGTAAAAA CAAGCCTGAT 
TCCAACCAGC TGATCCTGGA GCATGCGGGC ACCTTCTTTG ATGAGATCCA GCAGCGGCAT 
GGCCTGGCCA ACTCCATCTC CTCCTACCTA ATTAAGCCTG TCCAGAGGGT TACCAAGTAC 
CAGCTGCTCC TGAAGGAACT TCTAACTTGC TGTGAAGAAG GGAAAGGAGA GCTGAAGGAT 
GGCCTGGAGG TGATGCTCAG TGTCCCCAAG AAAGCCAACG ATGCCATGCA TGTCAGCATG 
CTGGAAGGGT TCGATGAAAA CTTGGATGTA CAGGGGGAGT TGATTCTCCA GGATGCCTTT 
CAAGTGTGGG ACCCAAAGTC GCTGATCCGG AAGGGGCGGG AGCGCCATCT GTTCCTCTTT 
GAGATCTCCT TGGTGTTTAG CAAAGAGATC AAAGACTCTT CAGGACACAC GAAATATGTT 
TACAAGAACA AGCTACTGAC CTCAGAGCTG GGTGTGACCG AGCATGTAGA AGGTGACCCC 
TGCAAATTCG CCTTGTGGTC TGGGCGCACC CCATCCTCAG ACAATAAAAC AGTGCTCAAA 
GCTTCCAACA TCGAAACCAA GCAGGAATGG ATCAAGAACA TTCGAGAAGT GATTCAGGAG 
AGGATCATTC ATCTTAAAGG CGCTTTAAAG GAGCCAATTC AGCTCCCCAA AACACCAGCC 
AAACTGAGGA ACAATAGTAA GAGGGATGGG GTAGAGGATG GTGACAGCCA AGGGGATGGG 
AGCAGCCAGC CAGATACCAT ATCCATTGCC TCCAGGACCT CTCAGAACAC AGTGGAAAGT 
GACAAGCTCT CAGGTGGATG TGAGCTGACC GTGGTCCTCC AGGACTTCAG CGCAGGCCAC 
AGCAGCGAGC TGAGCATCCA GGTGGGCCAG ACGGTGGAGC TGCTGGAACG ACCGAGTGAG 
AGGCCTGGCT GGTGTCTGGT CCGCACCACA GAGCGGAGCC CCCCACAGGA AGGCCTGGTA 
CCCAGCAGCG CCCTGTGCAT CTCACACTCT CGCAGCAGCG TGGAAATGGA CTGCTTCTTC 
CCCTTGAAAG ATTCATACTC TCATTCCTCC AGTGAGAATG GAGGCAAATC CGAGTCCGTG 
GCCCACCTGC AGTCTCAGCC CTCCCTGAAC TCCATCCACA GTTCCCCTGG TCCCAAGCGC 
TCCACCAACA CCCTCAAAAA GTGGCTGACC AGCCCCGTGC GCCGCCTCAA CAGCGGAAAA 
GCCGACGGGA ACATCAAAAA GCAGAAGAAA GTCCGGGATG GGCGGAAGAG CTTCGATCTG 
GGATCGCCCA AGCCTGGGGA TGAGACGACA CCTCAGGGAG ACAGCGCTGA CGAGAAGAGC 
AAGAAGGGTT GGGGCGAAGA CGAGCCAGAT GAAGAGTCAC ACACACCCCT CCCTCCGCCC 
ATGAAGATCT TTGACAACGA CCCCACACAG GATGAGATGT CGTCTTTGCT AGCAGCCCGG 
CAGGCTCCCC CTGACGTGCC TACTGCTGCA GACCTTGTCA GTGCAATAGA AAAGTTGGTC 
AAAAATAAGC TGACTCTGGA AGGAGGCTCT TACCGGGGGA GCTTGAAGGA CCCTACAGGC 
TGCCTGAATG AGGGGATGAC CCCTCCCACA CCTCCTAGAA ACCTGGAAGA AGAACAGAAA 
GCCAAGGCGC TGAGAGGCAG GATGTTTGTC CTGAATGAGC TGGTACAGAC AGAGAAGGAC 
TACGTCAAGG ACCTGGGGAT TGTGGTGGAG GGCTTCATGA AGAGAATAGA AGAGAAGGGG 
GTCCCTGAGG ACATGCGAGG GAAGGAAAAG ATCGTGTTTG GAAATATCCA CCAGATTTAC 
GACTGGCATA AAGATTTTTT CCTGGCTGAA CTGGAAAAAT GTATCCAAGA GCAAGACAGA 
TTGGCCCAGC TCTTCATTAA ACACGAGCGG AAGCTGCACA TCTACGTGTG GTACTGCCAG 
AACAAGCCGC GTTCCGAGTA CATCGTGGCT GAGTACGATG CCTATTTTGA GGAGGTTAAA 
CAAGAGATCA ATCAAAGGCT GACCCTTAGT GACTTCCTGA TCAAGCCCAT TCAGAGAATA 
ACAAAGTACC AGCTGCTACT CAAGGACTTC CTGCGATACA GCGAGAAGGC TGGTTTGGAG 
TGTTCCGATA TTGAGAAAGC AGTGGAGTTA ATGTGCCTTG TCCCCAAGCG CTGTAATGAC 
ATGATGAATC TGGGACGCCT GCAGGGCTTC GAGGGCACCC TGACCGCCCA GGGCAAGCTA 
CTGCAGCAGG ACACATTCTA TGTGATCGAG CTGGATGCCG GCATGCAGTC CCGGACCAAG 
GAGAGGCGTG TGTTTCTCTT TGAGCAGATT GTCATCTTCA GTGAACTGCT CCGAAAGGGG 
TCCCTCACCC CAGGCTACAT GTTCAAAAGA AGCATCAAGA TGAATTACTT GGTCCTGGAA 
GACAATGTGG ATGGTGACCC CTGCAAATTT GCACTCATGA ACAGGGAGAC TTCAGAAAGG 
GTCATTCTAC AGGCTGCCAA CTCAGACATC CAGCAGGCCT GGGTGCAGGA CATCAACCAA 
GTCCTAGAAA CACAGAGGGA CTTCTTAAAT GCACTACAGT CACCCATTGA GTATCAGCGG 
AAGGAGAGAA GCACAGCTGT GATCCGGTCC CAGCCCCCGA GGGTTCCACA AGCCAGCCCC 
AGGCCCTACT CTTCTGGCCC GGTGGGCTCA GAGAAGCCCC CCAAGGGCTC CAGCTATAAC 
CCGCCTCTGC CACCCCTGAA GATATCTACC TCTAATGGCA GTCCAGGGTT CGACTACCAT 
CAGCCTGGGG ACAAGTTTGA CGCTAGCAAG CAGAACGACC TGGGAGGCTG CAATGGGACA 
TCAACCATGA CTGTAATCAA AGATTACTAT GCACTGAAGG AGAATGAAAT CTGCGTGAGC 
CAAGGTGAGG TGGTACAGGT CCTCGCCGTC AACCAGCAGA ACATGTGTCT GGTGTACCAG 
CCTGCCAGCG ACCATTCCCC GGCAGCAGAG GGCTGGGTCC CAGGCAGCAT CCTGGCGCCC 
CTCGCCAAAG CCACAGCAGC GGCCGAAAGT AGTGACGGGA GCATCAAGAA GTCATGCTCT 
TGGCACACTC TACGCATGAG AAAGCGGGCA GACGTGGAGA ACTCGGGTAA AAATGAAGCC 
ACAGGGCCTC GTAAACCCAA GGATATTCTG GGCAACAAAG TCTCTGTTAA AGAGACGAAC 
AGTTCCGAGG AGTCAGAGTG TGATGATCTT GACCCTAATA CTAGTATGGA GATCTTGAAT 
CCAAATTTCA TCCAGGAAGT GGCTCCAGAA TTCCTCGTGC CCTTAGTAGA TGTGACCTGT 
TTGCTTGGAG ACACAGTGCT ACTGCAGTGT AAAGCCTGTG GGCGGCCAAA GCCCAGCATC 
ACCTGGAAGG GTCCGGATCA GAACATCCTA GACACTGACA ACAGTTCAGC AACATACACC 
ATCTCCTCCT GTGATTCTGG GGAAAGCACC CTCAAGATCT GCAACCTGAT GCCCCAGGAT 
AGTGGGATTT ATACCTGCAT AGCAGCAAAC GACCACGGGA CTGCATCCAC ATCTGCTACA 
GTTAAGGTGC AAGGTGTCCC CGCAGCCCCC AACCGCCCAA TCGCACAGGA GAGGAGCTGC 
ACCTCCGTCA TCCTCCGCTG GCTGCCCCCA GCCAGCACCG GAAACTGCAC TATTTCGGGG 
TACACAGTGG AGTATCGCGA GGAAGGTTCC CAGGTCTGGC AGCAATCCGT AGCTTCGACC 
TTGGACACTT ACCTGGTCAT TGAAGACCTT AGTCCTGGGT GCCCTTATCA GTTCAGGGTC 
AGTGCCAGCA ACCCCTGGGG AATCAGCCTT CCCAGTGAGC CCTCGGAGTT TGTGCGCCTT 
CCAGAGTACG ATGCAGCCGC CGACGGTGCC ACCATTTCTT GGAAGGAAAA TTTTGATTCT 
GCTTACACTG AACTGAACGA GATTGGCAGA GGCCGTTTCT CTATTGTAAA GAAATGCATT 
CACAAAGCTA CCCGCAAAGA TGTTGCTGTG AAATTTGTGA GCAAAAAAAT GAAGAAGAAG 
GAGCAGGCTG CTCACGAGGC CGCCCTGCTC CAGCACCTGC AGCACCCTCA GTATGTCACG 
CTCCATGACA CCTACGAGTC CCCCACATCC TACATCCTGA TTTTGGAACT GATGGATGAC 
GGCCGACTCT TGGACTACCT TATGAATCAT GATGAGCTGA TGGAAGAAAA GGTAGCTTTC 
TACATTCGAG ACATCATGGA AGCTCTGCAG TACCTGCACA ACTGTAGGGT CGCACATTTG 
GACATAAAGC CCGAAAACCT GCTCATTGAC CTCCGGATCC CAGTGCCTCG AGTGAAGCTC 
ATCGACTTGG AAGACGCTGT CCAGATCTCA GGCCACTTCC ATATCCACCA TCTGCTGGGG 
AACCCTGAGT TCGCTGCCCC CGAAGTGATC CAAGGCATCC CTGTCTCCCT GGGCACAGAC 
ATCTGGAGCA TCGGGGTTCT GACATATGTC ATGCTTAGTG GGGTCTCCCC CTTCTTAGAT 
GAGAGCAAAG AGGAAACCTG TATCAACGTG TGCAGGGTGG ACTTTAGCTT CCCCCACGAG 
TACTTCTGCG GTGTGAGCAA TGCTGCCAGG GATTTCATCA ACGTAATCTT ACAGGAAGAC 
TTCCGGAGGC GGCCCACAGC AGCCACGTGC CTGCAGCATC CCTGGCTACA GCCCCACAAC 
GGCAGCTACT CCAAGATCCC CTTGGACACC TCCCGTCTGG CCTGCTTCAT AGAACGCCGC 
AAGCACCAGA ATGATGTGCG GCCCATTCCC AATGTCAAGA GTTACATCGT CAATAGGGTG 
AACCAAGGGA CCTCGCTATC CCACAACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522381.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522444.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X29, mRNA.

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CloneID	OMu52297
Clone ID Related Accession (Same CDS sequence)	XM_006522381.1
Accession Version	XM_006522381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCT CTTTCCACAC GCACACCAAA GAGCTGTGGA CATGGATGGA GGACCTTCAG 
AAGGAGGTGC TGGAGGATGT CTGTGCAGAC TCTGTGGATG CGGTCCAGGA GCTGATCAAG 
CAGTTCCAGC AGCAGCAGAC CGCCACCCTG GATGCCACTC TCAATGTCAT CAAGGAAGGC 
GAAGACCTTA TCCAGCAGCT CAGGGACTCA GCGATGTCCA ACAACAAGAC ACCCCACAGT 
AGCTCTATCA GCCATATTGA GTCTGTCCTC CAGCAGCTGG ACGATGCTCA GGTACAGATG 
GAAGAGCTGT TTCACGAGCG GAAGATCAAG CTGGACATCT TCCTACAGCT GCGCATCTTT 
GAACAGTACA CCATTGAGGT CACTGCAGAG CTGGATGCCT GGAACGAGGA CTTGCTCCGG 
CAGATGAATG ATTTCAACAC AGAGGATCTG ACCCTGGCTG AGCAGCGGCT ACAGCGCCAC 
ACAGAACGGA AGCTTGCCAT GAACAACATG ACGTTCGAGG TCATCCAGCA GGGCCAGGAC 
CTGCACCAGT ACATCATGGA AGTGCAAGCG TCAGGAATCG AGTTAATCTG TGAGAAGGAC 
CTTGACCTGG CAGCTCAGGT GCAGGAGCTG CTGGAGTTTC TCCATGAGAA GCAGCACGAG 
CTGGAGCTCA ATGCGGAGCA GACGCACAAG CGGCTAGAGC AGTGTCTCCA GCTGAGGCAC 
CTCCAGGCTG AGGTCAAACA GGTTCTGGGA TGGATCCGCA ATGGAGAGTC GATGCTCAAC 
GCCAGCCTGG TCAACGCCAG CTCTTTGTCG GAGGCAGAGC AGCTGCAGCG GGAGCATGAA 
CAGTTCCAGC TAGCCATCGA GTCCCTCTTT CATGCCACTT CCTTGCAGAA GACGCACCAG 
AGCGCCCTGC AGGTACAGCA GAAGGCAGAG GCACTGCTCC AGGCCGGCCA CTACGACGCA 
GACGCCATCC GGGAATGTGC TGAGAAGGTG GCCCTCCACT GGCAACAGCT CATGCTAAAG 
ATGGAGGACC GGCTGAAGCT TGTCAACGCC TCTGTGGCCT TTTACAAGAC CTCTGAGCAG 
GTGTGCAGTG TTCTGGAGAG CTTGGAGCAG GAGTACCGGA GAGACGAGGA CTGGTGTGGT 
GGGAGAGACA AGCTGGGGCC GGCGGCAGAG ATGGACCACG TTATTCCACT CCTCAGCAAG 
CACCTGGAGC AGAAGGAGGC CTTCCTGAAG GCCTGCACGC TGGCAAGGCG GAACGCCGAG 
GTGTTCCTCA AATACATCCA CAGGAACAAT GTGAGCATGC CTAGTGTTGC CAGCCACACC 
CGGGGGCCTG AACAGCAAGT AAAAGCCATC TTGAGCGAGC TCTTGCAGAG GGAGAATCGA 
GTCCTGCACT TCTGGACCTT GAAGAAGCGG CGGTTAGACC AGTGTCAGCA ATATGTGGTG 
TTTGAGCGCA GTGCTAAGCA GGCACTGGAC TGGATCCAAG AAACAGGAGA ATATTACCTC 
TCAACACATA CCTCCACCGG AGAGACCACG GAGGAGACTC AGGAGCTGCT GAAAGAGTAT 
GGAGAATTCA GGGTGCCTGC CAAGCAAACG AAGGAGAAGG TGAAACTTCT GATCCAGCTA 
GCAGACAGCT TTGTGGAAAA GGGCCACATC CACGCCACAG AGATACGGAA ATGGGTGACC 
ACGGTGGACA AGCACTACCG CGATTTCTCC CTGAGGATGG GGAAGTACCG GTACTCGCTG 
GAGAAGGCGC TGGGGGTCAA CACGGAGGAT AACAAGGACC TAGAGCTGGA CATCATCCCA 
GCAAGCCTTT CGGATCGGGA GGTCAAGCTC CGGGACGCTA ACCATGAGAT CAATGAAGAG 
AAACGGAAGT CAGCTCGAAA GAAAGAGTTC ATTATGGCTG AACTGCTCCA AACTGAGAAG 
GCTTATGTAA GGGACTTGCA CGAGTGCTTG GAGACCTACC TGTGGGAGAT GACTAGTGGT 
GTGGAAGAGA TACCCCCCGG CATCCTCAAT AAGGAACATA TCATCTTCGG CAACATCCAG 
GAGATCTATG ACTTCCATAA CAACATCTTC CTCAAAGAGC TGGAGAAGTA CGAGCAGCTG 
CCTGAGGATG TGGGACACTG CTTTGTTACC TGGGCAGACA AATTTCAGAT GTATGTCACC 
TACTGTAAAA ACAAGCCTGA TTCCAACCAG CTGATCCTGG AGCATGCGGG CACCTTCTTT 
GATGAGATCC AGCAGCGGCA TGGCCTGGCC AACTCCATCT CCTCCTACCT AATTAAGCCT 
GTCCAGAGGG TTACCAAGTA CCAGCTGCTC CTGAAGGAAC TTCTAACTTG CTGTGAAGAA 
GGGAAAGGAG AGCTGAAGGA TGGCCTGGAG GTGATGCTCA GTGTCCCCAA GAAAGCCAAC 
GATGCCATGC ATGTCAGCAT GCTGGAAGGG TTCGATGAAA ACTTGGATGT ACAGGGGGAG 
TTGATTCTCC AGGATGCCTT TCAAGTGTGG GACCCAAAGT CGCTGATCCG GAAGGGGCGG 
GAGCGCCATC TGTTCCTCTT TGAGATCTCC TTGGTGTTTA GCAAAGAGAT CAAAGACTCT 
TCAGGACACA CGAAATATGT TTACAAGAAC AAGCTACTGA CCTCAGAGCT GGGTGTGACC 
GAGCATGTAG AAGGTGACCC CTGCAAATTC GCCTTGTGGT CTGGGCGCAC CCCATCCTCA 
GACAATAAAA CAGTGCTCAA AGCTTCCAAC ATCGAAACCA AGCAGGAATG GATCAAGAAC 
ATTCGAGAAG TGATTCAGGA GAGGATCATT CATCTTAAAG GCGCTTTAAA GGAGCCAATT 
CAGCTCCCCA AAACACCAGC CAAACTGAGG AACAATAGTA AGAGGGATGG GGTAGAGGAT 
GGTGACAGCC AAGGGGATGG GAGCAGCCAG CCAGATACCA TATCCATTGC CTCCAGGACC 
TCTCAGAACA CAGTGGAAAG TGACAAGCTC TCAGGTGGAT GTGAGCTGAC CGTGGTCCTC 
CAGGACTTCA GCGCAGGCCA CAGCAGCGAG CTGAGCATCC AGGTGGGCCA GACGGTGGAG 
CTGCTGGAAC GACCGAGTGA GAGGCCTGGC TGGTGTCTGG TCCGCACCAC AGAGCGGAGC 
CCCCCACAGG AAGGCCTGGT ACCCAGCAGC GCCCTGTGCA TCTCACACTC TCGCAGCAGC 
GTGGAAATGG ACTGCTTCTT CCCCTTGAAA GATTCATACT CTCATTCCTC CAGTGAGAAT 
GGAGGCAAAT CCGAGTCCGT GGCCCACCTG CAGTCTCAGC CCTCCCTGAA CTCCATCCAC 
AGTTCCCCTG GTCCCAAGCG CTCCACCAAC ACCCTCAAAA AGTGGCTGAC CAGCCCCGTG 
CGCCGCCTCA ACAGCGGAAA AGCCGACGGG AACATCAAAA AGCAGAAGAA AGTCCGGGAT 
GGGCGGAAGA GCTTCGATCT GGGATCGCCC AAGCCTGGGG ATGAGACGAC ACCTCAGGGA 
GACAGCGCTG ACGAGAAGAG CAAGAAGGGT TGGGGCGAAG ACGAGCCAGA TGAAGAGTCA 
CACACACCCC TCCCTCCGCC CATGAAGATC TTTGACAACG ACCCCACACA GGATGAGATG 
TCGTCTTTGC TAGCAGCCCG GCAGGCTCCC CCTGACGTGC CTACTGCTGC AGACCTTGTC 
AGTGCAATAG AAAAGTTGGT CAAAAATAAG CTGACTCTGG AAGGAGGCTC TTACCGGGGG 
AGCTTGAAGG ACCCTACAGG CTGCCTGAAT GAGGGGATGA CCCCTCCCAC ACCTCCTAGA 
AACCTGGAAG AAGAACAGAA AGCCAAGGCG CTGAGAGGCA GGATGTTTGT CCTGAATGAG 
CTGGTACAGA CAGAGAAGGA CTACGTCAAG GACCTGGGGA TTGTGGTGGA GGGCTTCATG 
AAGAGAATAG AAGAGAAGGG GGTCCCTGAG GACATGCGAG GGAAGGAAAA GATCGTGTTT 
GGAAATATCC ACCAGATTTA CGACTGGCAT AAAGATTTTT TCCTGGCTGA ACTGGAAAAA 
TGTATCCAAG AGCAAGACAG ATTGGCCCAG CTCTTCATTA AACACGAGCG GAAGCTGCAC 
ATCTACGTGT GGTACTGCCA GAACAAGCCG CGTTCCGAGT ACATCGTGGC TGAGTACGAT 
GCCTATTTTG AGGAGGTTAA ACAAGAGATC AATCAAAGGC TGACCCTTAG TGACTTCCTG 
ATCAAGCCCA TTCAGAGAAT AACAAAGTAC CAGCTGCTAC TCAAGGACTT CCTGCGATAC 
AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT ATTGAGAAAG CAGTGGAGTT AATGTGCCTT 
GTCCCCAAGC GCTGTAATGA CATGATGAAT CTGGGACGCC TGCAGGGCTT CGAGGGCACC 
CTGACCGCCC AGGGCAAGCT ACTGCAGCAG GACACATTCT ATGTGATCGA GCTGGATGCC 
GGCATGCAGT CCCGGACCAA GGAGAGGCGT GTGTTTCTCT TTGAGCAGAT TGTCATCTTC 
AGTGAACTGC TCCGAAAGGG GTCCCTCACC CCAGGCTACA TGTTCAAAAG AAGCATCAAG 
ATGAATTACT TGGTCCTGGA AGACAATGTG GATGGTGACC CCTGCAAATT TGCACTCATG 
AACAGGGAGA CTTCAGAAAG GGTCATTCTA CAGGCTGCCA ACTCAGACAT CCAGCAGGCC 
TGGGTGCAGG ACATCAACCA AGTCCTAGAA ACACAGAGGG ACTTCTTAAA TGCACTACAG 
TCACCCATTG AGTATCAGCG GAAGGAGAGA AGCACAGCTG TGATCCGGTC CCAGCCCCCG 
AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC 
CCCAAGGGCT CCAGCTATAA CCCGCCTCTG CCACCCCTGA AGATATCTAC CTCTAATGGC 
AGTCCAGGGT TCGACTACCA TCAGCCTGGG GACAAGTTTG ACGCTAGCAA GCAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAGA AGTCATGCTC TTGGCACACT CTACGCATGA GAAAGCGGGC AGACGTGGAG 
AACTCGGGTA AAAATGAAGC CACAGGGCCT CGTAAACCCA AGGATATTCT GGGCAACAAA 
GTCTCTGTTA AAGAGACGAA CAGTTCCGAG GAGTCAGAGT GTGATGATCT TGACCCTAAT 
ACTAGTATGG AGATCTTGAA TCCAAATTTC ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG 
CCCTTAGTAG ATGTGACCTG TTTGCTTGGA GACACAGTGC TACTGCAGTG TAAAGCCTGT 
GGGCGGCCAA AGCCCAGCAT CACCTGGAAG GGTCCGGATC AGAACATCCT AGACACTGAC 
AACAGTTCAG CAACATACAC CATCTCCTCC TGTGATTCTG GGGAAAGCAC CCTCAAGATC 
TGCAACCTGA TGCCCCAGGA TAGTGGGATT TATACCTGCA TAGCAGCAAA CGACCACGGG 
ACTGCATCCA CATCTGCTAC AGTTAAGGTG CAAGGTGTCC CCGCAGCCCC CAACCGCCCA 
ATCGCACAGG AGAGGAGCTG CACCTCCGTC ATCCTCCGCT GGCTGCCCCC AGCCAGCACC 
GGAAACTGCA CTATTTCGGG GTACACAGTG GAGTATCGCG AGGAAGGTTC CCAGGTCTGG 
CAGCAATCCG TAGCTTCGAC CTTGGACACT TACCTGGTCA TTGAAGACCT TAGTCCTGGG 
TGCCCTTATC AGTTCAGGGT CAGTGCCAGC AACCCCTGGG GAATCAGCCT TCCCAGTGAG 
CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC GATGCAGCCG CCGACGGTGC CACCATTTCT 
TGGAAGGAAA ATTTTGATTC TGCTTACACT GAACTGAACG AGATTGGCAG AGGCCGTTTC 
TCTATTGTAA AGAAATGCAT TCACAAAGCT ACCCGCAAAG ATGTTGCTGT GAAATTTGTG 
AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT GCTCACGAGG CCGCCCTGCT CCAGCACCTG 
CAGCACCCTC AGTATGTCAC GCTCCATGAC ACCTACGAGT CCCCCACATC CTACATCCTG 
ATTTTGGAAC TGATGGATGA CGGCCGACTC TTGGACTACC TTATGAATCA TGATGAGCTG 
ATGGAAGAAA AGGTAGCTTT CTACATTCGA GACATCATGG AAGCTCTGCA GTACCTGCAC 
AACTGTAGGG TCGCACATTT GGACATAAAG CCCGAAAACC TGCTCATTGA CCTCCGGATC 
CCAGTGCCTC GAGTGAAGCT CATCGACTTG GAAGACGCTG TCCAGATCTC AGGCCACTTC 
CATATCCACC ATCTGCTGGG GAACCCTGAG TTCGCTGCCC CCGAAGTGAT CCAAGGCATC 
CCTGTCTCCC TGGGCACAGA CATCTGGAGC ATCGGGGTTC TGACATATGT CATGCTTAGT 
GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA GAGGAAACCT GTATCAACGT GTGCAGGGTG 
GACTTTAGCT TCCCCCACGA GTACTTCTGC GGTGTGAGCA ATGCTGCCAG GGATTTCATC 
AACGTAATCT TACAGGAAGA CTTCCGGAGG CGGCCCACAG CAGCCACGTG CCTGCAGCAT 
CCCTGGCTAC AGCCCCACAA CGGCAGCTAC TCCAAGATCC CCTTGGACAC CTCCCGTCTG 
GCCTGCTTCA TAGAACGCCG CAAGCACCAG AATGATGTGC GGCCCATTCC CAATGTCAAG 
AGTTACATCG TCAATAGGGT GAACCAAGGG ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522444.1
CDS	310..7323
Translation	MSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_006522381.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522381.1

ORF Insert Sequence:

ATGTCGGTCT CTTTCCACAC GCACACCAAA GAGCTGTGGA CATGGATGGA GGACCTTCAG 
AAGGAGGTGC TGGAGGATGT CTGTGCAGAC TCTGTGGATG CGGTCCAGGA GCTGATCAAG 
CAGTTCCAGC AGCAGCAGAC CGCCACCCTG GATGCCACTC TCAATGTCAT CAAGGAAGGC 
GAAGACCTTA TCCAGCAGCT CAGGGACTCA GCGATGTCCA ACAACAAGAC ACCCCACAGT 
AGCTCTATCA GCCATATTGA GTCTGTCCTC CAGCAGCTGG ACGATGCTCA GGTACAGATG 
GAAGAGCTGT TTCACGAGCG GAAGATCAAG CTGGACATCT TCCTACAGCT GCGCATCTTT 
GAACAGTACA CCATTGAGGT CACTGCAGAG CTGGATGCCT GGAACGAGGA CTTGCTCCGG 
CAGATGAATG ATTTCAACAC AGAGGATCTG ACCCTGGCTG AGCAGCGGCT ACAGCGCCAC 
ACAGAACGGA AGCTTGCCAT GAACAACATG ACGTTCGAGG TCATCCAGCA GGGCCAGGAC 
CTGCACCAGT ACATCATGGA AGTGCAAGCG TCAGGAATCG AGTTAATCTG TGAGAAGGAC 
CTTGACCTGG CAGCTCAGGT GCAGGAGCTG CTGGAGTTTC TCCATGAGAA GCAGCACGAG 
CTGGAGCTCA ATGCGGAGCA GACGCACAAG CGGCTAGAGC AGTGTCTCCA GCTGAGGCAC 
CTCCAGGCTG AGGTCAAACA GGTTCTGGGA TGGATCCGCA ATGGAGAGTC GATGCTCAAC 
GCCAGCCTGG TCAACGCCAG CTCTTTGTCG GAGGCAGAGC AGCTGCAGCG GGAGCATGAA 
CAGTTCCAGC TAGCCATCGA GTCCCTCTTT CATGCCACTT CCTTGCAGAA GACGCACCAG 
AGCGCCCTGC AGGTACAGCA GAAGGCAGAG GCACTGCTCC AGGCCGGCCA CTACGACGCA 
GACGCCATCC GGGAATGTGC TGAGAAGGTG GCCCTCCACT GGCAACAGCT CATGCTAAAG 
ATGGAGGACC GGCTGAAGCT TGTCAACGCC TCTGTGGCCT TTTACAAGAC CTCTGAGCAG 
GTGTGCAGTG TTCTGGAGAG CTTGGAGCAG GAGTACCGGA GAGACGAGGA CTGGTGTGGT 
GGGAGAGACA AGCTGGGGCC GGCGGCAGAG ATGGACCACG TTATTCCACT CCTCAGCAAG 
CACCTGGAGC AGAAGGAGGC CTTCCTGAAG GCCTGCACGC TGGCAAGGCG GAACGCCGAG 
GTGTTCCTCA AATACATCCA CAGGAACAAT GTGAGCATGC CTAGTGTTGC CAGCCACACC 
CGGGGGCCTG AACAGCAAGT AAAAGCCATC TTGAGCGAGC TCTTGCAGAG GGAGAATCGA 
GTCCTGCACT TCTGGACCTT GAAGAAGCGG CGGTTAGACC AGTGTCAGCA ATATGTGGTG 
TTTGAGCGCA GTGCTAAGCA GGCACTGGAC TGGATCCAAG AAACAGGAGA ATATTACCTC 
TCAACACATA CCTCCACCGG AGAGACCACG GAGGAGACTC AGGAGCTGCT GAAAGAGTAT 
GGAGAATTCA GGGTGCCTGC CAAGCAAACG AAGGAGAAGG TGAAACTTCT GATCCAGCTA 
GCAGACAGCT TTGTGGAAAA GGGCCACATC CACGCCACAG AGATACGGAA ATGGGTGACC 
ACGGTGGACA AGCACTACCG CGATTTCTCC CTGAGGATGG GGAAGTACCG GTACTCGCTG 
GAGAAGGCGC TGGGGGTCAA CACGGAGGAT AACAAGGACC TAGAGCTGGA CATCATCCCA 
GCAAGCCTTT CGGATCGGGA GGTCAAGCTC CGGGACGCTA ACCATGAGAT CAATGAAGAG 
AAACGGAAGT CAGCTCGAAA GAAAGAGTTC ATTATGGCTG AACTGCTCCA AACTGAGAAG 
GCTTATGTAA GGGACTTGCA CGAGTGCTTG GAGACCTACC TGTGGGAGAT GACTAGTGGT 
GTGGAAGAGA TACCCCCCGG CATCCTCAAT AAGGAACATA TCATCTTCGG CAACATCCAG 
GAGATCTATG ACTTCCATAA CAACATCTTC CTCAAAGAGC TGGAGAAGTA CGAGCAGCTG 
CCTGAGGATG TGGGACACTG CTTTGTTACC TGGGCAGACA AATTTCAGAT GTATGTCACC 
TACTGTAAAA ACAAGCCTGA TTCCAACCAG CTGATCCTGG AGCATGCGGG CACCTTCTTT 
GATGAGATCC AGCAGCGGCA TGGCCTGGCC AACTCCATCT CCTCCTACCT AATTAAGCCT 
GTCCAGAGGG TTACCAAGTA CCAGCTGCTC CTGAAGGAAC TTCTAACTTG CTGTGAAGAA 
GGGAAAGGAG AGCTGAAGGA TGGCCTGGAG GTGATGCTCA GTGTCCCCAA GAAAGCCAAC 
GATGCCATGC ATGTCAGCAT GCTGGAAGGG TTCGATGAAA ACTTGGATGT ACAGGGGGAG 
TTGATTCTCC AGGATGCCTT TCAAGTGTGG GACCCAAAGT CGCTGATCCG GAAGGGGCGG 
GAGCGCCATC TGTTCCTCTT TGAGATCTCC TTGGTGTTTA GCAAAGAGAT CAAAGACTCT 
TCAGGACACA CGAAATATGT TTACAAGAAC AAGCTACTGA CCTCAGAGCT GGGTGTGACC 
GAGCATGTAG AAGGTGACCC CTGCAAATTC GCCTTGTGGT CTGGGCGCAC CCCATCCTCA 
GACAATAAAA CAGTGCTCAA AGCTTCCAAC ATCGAAACCA AGCAGGAATG GATCAAGAAC 
ATTCGAGAAG TGATTCAGGA GAGGATCATT CATCTTAAAG GCGCTTTAAA GGAGCCAATT 
CAGCTCCCCA AAACACCAGC CAAACTGAGG AACAATAGTA AGAGGGATGG GGTAGAGGAT 
GGTGACAGCC AAGGGGATGG GAGCAGCCAG CCAGATACCA TATCCATTGC CTCCAGGACC 
TCTCAGAACA CAGTGGAAAG TGACAAGCTC TCAGGTGGAT GTGAGCTGAC CGTGGTCCTC 
CAGGACTTCA GCGCAGGCCA CAGCAGCGAG CTGAGCATCC AGGTGGGCCA GACGGTGGAG 
CTGCTGGAAC GACCGAGTGA GAGGCCTGGC TGGTGTCTGG TCCGCACCAC AGAGCGGAGC 
CCCCCACAGG AAGGCCTGGT ACCCAGCAGC GCCCTGTGCA TCTCACACTC TCGCAGCAGC 
GTGGAAATGG ACTGCTTCTT CCCCTTGAAA GATTCATACT CTCATTCCTC CAGTGAGAAT 
GGAGGCAAAT CCGAGTCCGT GGCCCACCTG CAGTCTCAGC CCTCCCTGAA CTCCATCCAC 
AGTTCCCCTG GTCCCAAGCG CTCCACCAAC ACCCTCAAAA AGTGGCTGAC CAGCCCCGTG 
CGCCGCCTCA ACAGCGGAAA AGCCGACGGG AACATCAAAA AGCAGAAGAA AGTCCGGGAT 
GGGCGGAAGA GCTTCGATCT GGGATCGCCC AAGCCTGGGG ATGAGACGAC ACCTCAGGGA 
GACAGCGCTG ACGAGAAGAG CAAGAAGGGT TGGGGCGAAG ACGAGCCAGA TGAAGAGTCA 
CACACACCCC TCCCTCCGCC CATGAAGATC TTTGACAACG ACCCCACACA GGATGAGATG 
TCGTCTTTGC TAGCAGCCCG GCAGGCTCCC CCTGACGTGC CTACTGCTGC AGACCTTGTC 
AGTGCAATAG AAAAGTTGGT CAAAAATAAG CTGACTCTGG AAGGAGGCTC TTACCGGGGG 
AGCTTGAAGG ACCCTACAGG CTGCCTGAAT GAGGGGATGA CCCCTCCCAC ACCTCCTAGA 
AACCTGGAAG AAGAACAGAA AGCCAAGGCG CTGAGAGGCA GGATGTTTGT CCTGAATGAG 
CTGGTACAGA CAGAGAAGGA CTACGTCAAG GACCTGGGGA TTGTGGTGGA GGGCTTCATG 
AAGAGAATAG AAGAGAAGGG GGTCCCTGAG GACATGCGAG GGAAGGAAAA GATCGTGTTT 
GGAAATATCC ACCAGATTTA CGACTGGCAT AAAGATTTTT TCCTGGCTGA ACTGGAAAAA 
TGTATCCAAG AGCAAGACAG ATTGGCCCAG CTCTTCATTA AACACGAGCG GAAGCTGCAC 
ATCTACGTGT GGTACTGCCA GAACAAGCCG CGTTCCGAGT ACATCGTGGC TGAGTACGAT 
GCCTATTTTG AGGAGGTTAA ACAAGAGATC AATCAAAGGC TGACCCTTAG TGACTTCCTG 
ATCAAGCCCA TTCAGAGAAT AACAAAGTAC CAGCTGCTAC TCAAGGACTT CCTGCGATAC 
AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT ATTGAGAAAG CAGTGGAGTT AATGTGCCTT 
GTCCCCAAGC GCTGTAATGA CATGATGAAT CTGGGACGCC TGCAGGGCTT CGAGGGCACC 
CTGACCGCCC AGGGCAAGCT ACTGCAGCAG GACACATTCT ATGTGATCGA GCTGGATGCC 
GGCATGCAGT CCCGGACCAA GGAGAGGCGT GTGTTTCTCT TTGAGCAGAT TGTCATCTTC 
AGTGAACTGC TCCGAAAGGG GTCCCTCACC CCAGGCTACA TGTTCAAAAG AAGCATCAAG 
ATGAATTACT TGGTCCTGGA AGACAATGTG GATGGTGACC CCTGCAAATT TGCACTCATG 
AACAGGGAGA CTTCAGAAAG GGTCATTCTA CAGGCTGCCA ACTCAGACAT CCAGCAGGCC 
TGGGTGCAGG ACATCAACCA AGTCCTAGAA ACACAGAGGG ACTTCTTAAA TGCACTACAG 
TCACCCATTG AGTATCAGCG GAAGGAGAGA AGCACAGCTG TGATCCGGTC CCAGCCCCCG 
AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC 
CCCAAGGGCT CCAGCTATAA CCCGCCTCTG CCACCCCTGA AGATATCTAC CTCTAATGGC 
AGTCCAGGGT TCGACTACCA TCAGCCTGGG GACAAGTTTG ACGCTAGCAA GCAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAGA AGTCATGCTC TTGGCACACT CTACGCATGA GAAAGCGGGC AGACGTGGAG 
AACTCGGGTA AAAATGAAGC CACAGGGCCT CGTAAACCCA AGGATATTCT GGGCAACAAA 
GTCTCTGTTA AAGAGACGAA CAGTTCCGAG GAGTCAGAGT GTGATGATCT TGACCCTAAT 
ACTAGTATGG AGATCTTGAA TCCAAATTTC ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG 
CCCTTAGTAG ATGTGACCTG TTTGCTTGGA GACACAGTGC TACTGCAGTG TAAAGCCTGT 
GGGCGGCCAA AGCCCAGCAT CACCTGGAAG GGTCCGGATC AGAACATCCT AGACACTGAC 
AACAGTTCAG CAACATACAC CATCTCCTCC TGTGATTCTG GGGAAAGCAC CCTCAAGATC 
TGCAACCTGA TGCCCCAGGA TAGTGGGATT TATACCTGCA TAGCAGCAAA CGACCACGGG 
ACTGCATCCA CATCTGCTAC AGTTAAGGTG CAAGGTGTCC CCGCAGCCCC CAACCGCCCA 
ATCGCACAGG AGAGGAGCTG CACCTCCGTC ATCCTCCGCT GGCTGCCCCC AGCCAGCACC 
GGAAACTGCA CTATTTCGGG GTACACAGTG GAGTATCGCG AGGAAGGTTC CCAGGTCTGG 
CAGCAATCCG TAGCTTCGAC CTTGGACACT TACCTGGTCA TTGAAGACCT TAGTCCTGGG 
TGCCCTTATC AGTTCAGGGT CAGTGCCAGC AACCCCTGGG GAATCAGCCT TCCCAGTGAG 
CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC GATGCAGCCG CCGACGGTGC CACCATTTCT 
TGGAAGGAAA ATTTTGATTC TGCTTACACT GAACTGAACG AGATTGGCAG AGGCCGTTTC 
TCTATTGTAA AGAAATGCAT TCACAAAGCT ACCCGCAAAG ATGTTGCTGT GAAATTTGTG 
AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT GCTCACGAGG CCGCCCTGCT CCAGCACCTG 
CAGCACCCTC AGTATGTCAC GCTCCATGAC ACCTACGAGT CCCCCACATC CTACATCCTG 
ATTTTGGAAC TGATGGATGA CGGCCGACTC TTGGACTACC TTATGAATCA TGATGAGCTG 
ATGGAAGAAA AGGTAGCTTT CTACATTCGA GACATCATGG AAGCTCTGCA GTACCTGCAC 
AACTGTAGGG TCGCACATTT GGACATAAAG CCCGAAAACC TGCTCATTGA CCTCCGGATC 
CCAGTGCCTC GAGTGAAGCT CATCGACTTG GAAGACGCTG TCCAGATCTC AGGCCACTTC 
CATATCCACC ATCTGCTGGG GAACCCTGAG TTCGCTGCCC CCGAAGTGAT CCAAGGCATC 
CCTGTCTCCC TGGGCACAGA CATCTGGAGC ATCGGGGTTC TGACATATGT CATGCTTAGT 
GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA GAGGAAACCT GTATCAACGT GTGCAGGGTG 
GACTTTAGCT TCCCCCACGA GTACTTCTGC GGTGTGAGCA ATGCTGCCAG GGATTTCATC 
AACGTAATCT TACAGGAAGA CTTCCGGAGG CGGCCCACAG CAGCCACGTG CCTGCAGCAT 
CCCTGGCTAC AGCCCCACAA CGGCAGCTAC TCCAAGATCC CCTTGGACAC CTCCCGTCTG 
GCCTGCTTCA TAGAACGCCG CAAGCACCAG AATGATGTGC GGCCCATTCC CAATGTCAAG 
AGTTACATCG TCAATAGGGT GAACCAAGGG ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245963.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244265.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X17, mRNA.

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CloneID	OMu75318
Clone ID Related Accession (Same CDS sequence)	XM_011245963.2 , XM_011245963.3
Accession Version	XM_011245963.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245963.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAGAAGTC ATGCTCTTGG CACACTCTAC GCATGAGAAA GCGGGCAGAC 
GTGGAGAACT CGGGTAAAAA TGAAGCCACA GGGCCTCGTA AACCCAAGGA TATTCTGGGC 
AACAAAGTCT CTGTTAAAAA ACAATGTGAT CATGATCCGC CTTCAGAAAT GCTTTGCCTT 
GGGACAATGA AAATCACTCA TTGGAGCTGG AGAGATGGCT CAGAGGTTAA GAGCACTGTC 
TGCTCTTCCA GAGGTCCTGA GTTTAATTCC CAGCAACCTT ATAGTGGCTC ACAACCATCT 
ATAATGAGAT CTGGTGCCCT CTTCTGGCTG CAGGCCATAA TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244265.1
CDS	4891..12435
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKKQCDHDPPSEMLCLGTMKITHWSWRDGSEVKSTVCSSRGPEFNSQQPYSGSQPSIMRSGALFWLQAII

Target ORF information:

RefSeq Version	XM_011245963.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245963.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAGAAGTC ATGCTCTTGG CACACTCTAC GCATGAGAAA GCGGGCAGAC 
GTGGAGAACT CGGGTAAAAA TGAAGCCACA GGGCCTCGTA AACCCAAGGA TATTCTGGGC 
AACAAAGTCT CTGTTAAAAA ACAATGTGAT CATGATCCGC CTTCAGAAAT GCTTTGCCTT 
GGGACAATGA AAATCACTCA TTGGAGCTGG AGAGATGGCT CAGAGGTTAA GAGCACTGTC 
TGCTCTTCCA GAGGTCCTGA GTTTAATTCC CAGCAACCTT ATAGTGGCTC ACAACCATCT 
ATAATGAGAT CTGGTGCCCT CTTCTGGCTG CAGGCCATAA TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245965.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244267.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X19, mRNA.

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CloneID	OMu75320
Clone ID Related Accession (Same CDS sequence)	XM_011245965.3 , XM_011245965.2
Accession Version	XM_011245965.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAAACCCT ACTGAAACCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244267.1
CDS	4891..12123
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKTLLKP

Target ORF information:

RefSeq Version	XM_011245965.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245965.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAAACCCT ACTGAAACCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245966.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244268.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X20, mRNA.

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CloneID	OMu75321
Clone ID Related Accession (Same CDS sequence)	XM_011245966.2 , XM_011245966.3
Accession Version	XM_011245966.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAAAAAGA ACCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244268.1
CDS	4891..12120
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKEPY

Target ORF information:

RefSeq Version	XM_011245966.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245966.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAAAAAGA ACCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245967.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244269.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X22, mRNA.

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CloneID	OMu75322
Clone ID Related Accession (Same CDS sequence)	XM_011245967.3 , XM_011245967.2
Accession Version	XM_011245967.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245967.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AAAAAGAACC CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244269.1
CDS	4891..12117
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKEPY

Target ORF information:

RefSeq Version	XM_011245967.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245967.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AAAAAGAACC CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( NM_177357.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796331.2 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant 1, mRNA.

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CloneID	OMu21881
Clone ID Related Accession (Same CDS sequence)	NM_177357.3
Accession Version	NM_177357.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	kalirin isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154588.2, CT010573.10, AC154524.2 and AC165079.2. On Apr 23, 2010 this sequence version replaced NM_177357.2. Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript was available for the full length of the gene. The genomic coordinates used for the transcript record were based on transcript alignments and orthologous data, including rat AF232669.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGAT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC TATCCTAAAG 
GAGAAAGTGG CCTTTGTGTC TGGGGGGCGT GACAAGCGAG GTGGACCCAT CCTGACCTTC 
CCTGCCCGAA GCAATCATGA CAGAATAAGA CAGGAAGACC TACGGAAACT TGTGACGTAT 
TTGGCCAGCG TGCCAAGTGA GGACGTTTGC AAACGTGGCT TCACCGTCAT CATCGACATG 
CGGGGCTCCA AGTGGGATCT CATCAAGCCC CTCCTCAAGA CGCTGCAGGA AGCCTTCCCC 
GCCGAGATCC ATGTGGCCCT CATCATCAAG CCTGACAACT TCTGGCAGAA GCAGAAGACC 
AACTTTGGCA GCTCCAAATT CATCTTTGAG ACAAGCATGG TGTCTGTGGA GGGCCTCACG 
AAGCTGGTGG ACCCCTCCCA GCTGACAGAG GAGTTTGACG GCTCCCTGGA TTACAACCAT 
GAGGAGTGGA TTGAGTTGCG CCTCTCCCTG GAGGAGTTCT TCAACAGTGC GGTCCACCTG 
CTCTCACGCC TTGAGGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC TGTGGATGTG 
GAGGGCTCTC GGAGGCTTAT CGACGAGCAC ACGCAACTTA AGAAAAAGGT GCTGAAGGCC 
CCCGTGGAAG AGCTGGACCG GGAGGGGCAG CGTCTACTGC AGTGCATCCG CTGCAGCGAT 
GGCTTCTCAG GGCGCAACTG CATCCCAGGA AGTGCCGACT TCCAGAGCCT TGTACCCAAG 
ATCACCAGCC TCCTGGACAA GCTGCATTCC ACCCGACAAC ACCTGCACCA AATGTGGCAC 
GTGCGAAAGC TCAAGCTGGA TCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAG 
AAGATGTTCG ACTGGATAAG CCACAACAAG GAGTTATTTC TTCAGAGCCA CACAGAGATC 
GGAGTCAGCT ACCAGCATGC ACTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC 
TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TCGCTTCCCG CCTCTCAGAG 
GCCGGCCATT ATGCCTCCCA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG 
AAGAGCTTTG CCGCTGCCCT GGATGAACGC AGTACAATCC TGGCCATGTC TGCTGTGTTC 
CACCAGAAGG CAGAGCAGTT CCTGTCAGGC GTGGATGCCT GGTGTAAGAT GTGCAGCGAA 
GGCGGCCTTC CTTCTGAGAT GCAGGACCTG GAGCTGGCAA TCCACCATCA CCAGTCCTTA 
TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ATGGCAAAGC ACTACTGGAT 
GTCCTGCAGC GTCCCCTGAG TCCTGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC 
TCCAAGGCGG TCCACCAGGT GCTGGATGTG GTCCACGAGG TGCTGCACCA TCAGCGACGG 
CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTTCACC AGCGGCTGCA GCTGTGTGTC 
TTCCAGCAGG ATGTGCAGCA GGTGCTGGAC TGGATTGAAA ACCATGGCGA GGCCTTTCTC 
AGCAAACACA CTGGGGTTGG CAAGTCCTTG CATCGAGCAC GTGCACTACA GAAGAGGCAT 
GATGACTTTG AAGAAGTGGC ACAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCA 
GCGGAGCAGC TGGCTCAGAC TGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCCCGG 
CACCTGGAGG TGCGCATCCA AGACTTTGTG CGCAGAGTGG AGCAGCGGAA GCTGCTTCTG 
GACATGTCGG TCTCTTTCCA CACGCACACC AAAGAGCTGT GGACATGGAT GGAGGACCTT 
CAGAAGGAGG TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAGCTGATC 
AAGCAGTTCC AGCAGCAGCA GACCGCCACC CTGGATGCCA CTCTCAATGT CATCAAGGAA 
GGCGAAGACC TTATCCAGCA GCTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCACCGAG 
GCCAGGGACT CAGCGATGTC CAACAACAAG ACACCCCACA GTAGCTCTAT CAGCCATATT 
GAGTCTGTCC TCCAGCAGCT GGACGATGCT CAGGTACAGA TGGAAGAGCT GTTTCACGAG 
CGGAAGATCA AGCTGGACAT CTTCCTACAG CTGCGCATCT TTGAACAGTA CACCATTGAG 
GTCACTGCAG AGCTGGATGC CTGGAACGAG GACTTGCTCC GGCAGATGAA TGATTTCAAC 
ACAGAGGATC TGACCCTGGC TGAGCAGCGG CTACAGCGCC ACACAGAACG GAAGCTTGCC 
ATGAACAACA TGACGTTCGA GGTCATCCAG CAGGGCCAGG ACCTGCACCA GTACATCATG 
GAAGTGCAAG CGTCAGGAAT CGAGTTAATC TGTGAGAAGG ACCTTGACCT GGCAGCTCAG 
GTGCAGGAGC TGCTGGAGTT TCTCCATGAG AAGCAGCACG AGCTGGAGCT CAATGCGGAG 
CAGACGCACA AGCGGCTAGA GCAGTGTCTC CAGCTGAGGC ACCTCCAGGC TGAGGTCAAA 
CAGGTTCTGG GATGGATCCG CAATGGAGAG TCGATGCTCA ACGCCAGCCT GGTCAACGCC 
AGCTCTTTGT CGGAGGCAGA GCAGCTGCAG CGGGAGCATG AACAGTTCCA GCTAGCCATC 
GAGAAGACGC ACCAGAGCGC CCTGCAGGTA CAGCAGAAGG CAGAGGCACT GCTCCAGGCC 
GGCCACTACG ACGCAGACGC CATCCGGGAA TGTGCTGAGA AGGTGGCCCT CCACTGGCAA 
CAGCTCATGC TAAAGATGGA GGACCGGCTG AAGCTTGTCA ACGCCTCTGT GGCCTTTTAC 
AAGACCTCTG AGCAGGTGTG CAGTGTTCTG GAGAGCTTGG AGCAGGAGTA CCGGAGAGAC 
GAGGACTGGT GTGGTGGGAG AGACAAGCTG GGGCCGGCGG CAGAGATGGA CCACGTTATT 
CCACTCCTCA GCAAGCACCT GGAGCAGAAG GAGGCCTTCC TGAAGGCCTG CACGCTGGCA 
AGGCGGAACG CCGAGGTGTT CCTCAAATAC ATCCACAGGA ACAATGTGAG CATGCCTAGT 
GTTGCCAGCC ACACCCGGGG GCCTGAACAG CAAGTAAAAG CCATCTTGAG CGAGCTCTTG 
CAGAGGGAGA ATCGAGTCCT GCACTTCTGG ACCTTGAAGA AGCGGCGGTT AGACCAGTGT 
CAGCAATATG TGGTGTTTGA GCGCAGTGCT AAGCAGGCAC TGGACTGGAT CCAAGAAACA 
GGAGAATATT ACCTCTCAAC ACATACCTCC ACCGGAGAGA CCACGGAGGA GACTCAGGAG 
CTGCTGAAAG AGTATGGAGA ATTCAGGGTG CCTGCCAAGC AAACGAAGGA GAAGGTGAAA 
CTTCTGATCC AGCTAGCAGA CAGCTTTGTG GAAAAGGGCC ACATCCACGC CACAGAGATA 
CGGAAATGGG TGACCACGGT GGACAAGCAC TACCGCGATT TCTCCCTGAG GATGGGGAAG 
TACCGGTACT CGCTGGAGAA GGCGCTGGGG GTCAACACGG AGGATAACAA GGACCTAGAG 
CTGGACATCA TCCCAGCAAG CCTTTCGGAT CGGGAGGTCA AGCTCCGGGA CGCTAACCAT 
GAGATCAATG AAGAGAAACG GAAGTCAGCT CGAAAGAAAG AGTTCATTAT GGCTGAACTG 
CTCCAAACTG AGAAGGCTTA TGTAAGGGAC TTGCACGAGT GCTTGGAGAC CTACCTGTGG 
GAGATGACTA GTGGTGTGGA AGAGATACCC CCCGGCATCC TCAATAAGGA ACATATCATC 
TTCGGCAACA TCCAGGAGAT CTATGACTTC CATAACAACA TCTTCCTCAA AGAGCTGGAG 
AAGTACGAGC AGCTGCCTGA GGATGTGGGA CACTGCTTTG TTACCTGGGC AGACAAATTT 
CAGATGTATG TCACCTACTG TAAAAACAAG CCTGATTCCA ACCAGCTGAT CCTGGAGCAT 
GCGGGCACCT TCTTTGATGA GATCCAGCAG CGGCATGGCC TGGCCAACTC CATCTCCTCC 
TACCTAATTA AGCCTGTCCA GAGGGTTACC AAGTACCAGC TGCTCCTGAA GGAACTTCTA 
ACTTGCTGTG AAGAAGGGAA AGGAGAGCTG AAGGATGGCC TGGAGGTGAT GCTCAGTGTC 
CCCAAGAAAG CCAACGATGC CATGCATGTC AGCATGCTGG AAGGGTTCGA TGAAAACTTG 
GATGTACAGG GGGAGTTGAT TCTCCAGGAT GCCTTTCAAG TGTGGGACCC AAAGTCGCTG 
ATCCGGAAGG GGCGGGAGCG CCATCTGTTC CTCTTTGAGA TCTCCTTGGT GTTTAGCAAA 
GAGATCAAAG ACTCTTCAGG ACACACGAAA TATGTTTACA AGAACAAGCT ACTGACCTCA 
GAGCTGGGTG TGACCGAGCA TGTAGAAGGT GACCCCTGCA AATTCGCCTT GTGGTCTGGG 
CGCACCCCAT CCTCAGACAA TAAAACAGTG CTCAAAGCTT CCAACATCGA AACCAAGCAG 
GAATGGATCA AGAACATTCG AGAAGTGATT CAGGAGAGGA TCATTCATCT TAAAGGCGCT 
TTAAAGGAGC CAATTCAGCT CCCCAAAACA CCAGCCAAAC TGAGGAACAA TAGTAAGAGG 
GATGGGGTAG AGGATGGTGA CAGCCAAGGG GATGGGAGCA GCCAGCCAGA TACCATATCC 
ATTGCCTCCA GGACCTCTCA GAACACAGTG GAAAGTGACA AGCTCTCAGG TGGATGTGAG 
CTGACCGTGG TCCTCCAGGA CTTCAGCGCA GGCCACAGCA GCGAGCTGAG CATCCAGGTG 
GGCCAGACGG TGGAGCTGCT GGAACGACCG AGTGAGAGGC CTGGCTGGTG TCTGGTCCGC 
ACCACAGAGC GGAGCCCCCC ACAGGAAGGC CTGGTACCCA GCAGCGCCCT GTGCATCTCA 
CACTCTCGCA GCAGCGTGGA AATGGACTGC TTCTTCCCCT TGAAAGATTC ATACTCTCAT 
TCCTCCAGTG AGAATGGAGG CAAATCCGAG TCCGTGGCCC ACCTGCAGTC TCAGCCCTCC 
CTGAACTCCA TCCACAGTTC CCCTGGTCCC AAGCGCTCCA CCAACACCCT CAAAAAGTGG 
CTGACCAGCC CCGTGCGCCG CCTCAACAGC GGAAAAGCCG ACGGGAACAT CAAAAAGCAG 
AAGAAAGTCC GGGATGGGCG GAAGAGCTTC GATCTGGGAT CGCCCAAGCC TGGGGATGAG 
ACGACACCTC AGGGAGACAG CGCTGACGAG AAGAGCAAGA AGGGTTGGGG CGAAGACGAG 
CCAGATGAAG AGTCACACAC ACCCCTCCCT CCGCCCATGA AGATCTTTGA CAACGACCCC 
ACACAGGATG AGATGTCGTC TTTGCTAGCA GCCCGGCAGG CTCCCCCTGA CGTGCCTACT 
GCTGCAGACC TTGTCAGTGC AATAGAAAAG TTGGTCAAAA ATAAGCTGAC TCTGGAAGGA 
GGCTCTTACC GGGGGAGCTT GAAGGACCCT ACAGGCTGCC TGAATGAGGG GATGACCCCT 
CCCACACCTC CTAGAAACCT GGAAGAAGAA CAGAAAGCCA AGGCGCTGAG AGGCAGGATG 
TTTGTCCTGA ATGAGCTGGT ACAGACAGAG AAGGACTACG TCAAGGACCT GGGGATTGTG 
GTGGAGGGCT TCATGAAGAG AATAGAAGAG AAGGGGGTCC CTGAGGACAT GCGAGGGAAG 
GAAAAGATCG TGTTTGGAAA TATCCACCAG ATTTACGACT GGCATAAAGA TTTTTTCCTG 
GCTGAACTGG AAAAATGTAT CCAAGAGCAA GACAGATTGG CCCAGCTCTT CATTAAACAC 
GAGCGGAAGC TGCACATCTA CGTGTGGTAC TGCCAGAACA AGCCGCGTTC CGAGTACATC 
GTGGCTGAGT ACGATGCCTA TTTTGAGGAG GTTAAACAAG AGATCAATCA AAGGCTGACC 
CTTAGTGACT TCCTGATCAA GCCCATTCAG AGAATAACAA AGTACCAGCT GCTACTCAAG 
GACTTCCTGC GATACAGCGA GAAGGCTGGT TTGGAGTGTT CCGATATTGA GAAAGCAGTG 
GAGTTAATGT GCCTTGTCCC CAAGCGCTGT AATGACATGA TGAATCTGGG ACGCCTGCAG 
GGCTTCGAGG GCACCCTGAC CGCCCAGGGC AAGCTACTGC AGCAGGACAC ATTCTATGTG 
ATCGAGCTGG ATGCCGGCAT GCAGTCCCGG ACCAAGGAGA GGCGTGTGTT TCTCTTTGAG 
CAGATTGTCA TCTTCAGTGA ACTGCTCCGA AAGGGGTCCC TCACCCCAGG CTACATGTTC 
AAAAGAAGCA TCAAGATGAA TTACTTGGTC CTGGAAGACA ATGTGGATGG TGACCCCTGC 
AAATTTGCAC TCATGAACAG GGAGACTTCA GAAAGGGTCA TTCTACAGGC TGCCAACTCA 
GACATCCAGC AGGCCTGGGT GCAGGACATC AACCAAGTCC TAGAAACACA GAGGGACTTC 
TTAAATGCAC TACAGTCACC CATTGAGTAT CAGCGGAAGG AGAGAAGCAC AGCTGTGATC 
CGGTCCCAGC CCCCGAGGGT TCCACAAGCC AGCCCCAGGC CCTACTCTTC TGGCCCGGTG 
GGCTCAGAGA AGCCCCCCAA GGGCTCCAGC TATAACCCGC CTCTGCCACC CCTGAAGATA 
TCTACCTCTA ATGGCAGTCC AGGGTTCGAC TACCATCAGC CTGGGGACAA GTTTGACGCT 
AGCAAGCAGA ACGACCTGGG AGGCTGCAAT GGGACATCAA CCATGACTGT AATCAAAGAT 
TACTATGCAC TGAAGGAGAA TGAAATCTGC GTGAGCCAAG GTGAGGTGGT ACAGGTCCTC 
GCCGTCAACC AGCAGAACAT GTGTCTGGTG TACCAGCCTG CCAGCGACCA TTCCCCGGCA 
GCAGAGGGCT GGGTCCCAGG CAGCATCCTG GCGCCCCTCG CCAAAGCCAC AGCAGCGGCC 
GAAAGTAGTG ACGGGAGCAT CAAGAAGTCA TGCTCTTGGC ACACTCTACG CATGAGAAAG 
CGGGCAGACG TGGAGAACTC GGGTAAAAAT GAAGCCACAG GGCCTCGTAA ACCCAAGGAT 
ATTCTGGGCA ACAAAGTCTC TGTTAAAGAG ACGAACAGTT CCGAGGAGTC AGAGTGTGAT 
GATCTTGACC CTAATACTAG TATGGAGATC TTGAATCCAA ATTTCATCCA GGAAGTGGCT 
CCAGAATTCC TCGTGCCCTT AGTAGATGTG ACCTGTTTGC TTGGAGACAC AGTGCTACTG 
CAGTGTAAAG CCTGTGGGCG GCCAAAGCCC AGCATCACCT GGAAGGGTCC GGATCAGAAC 
ATCCTAGACA CTGACAACAG TTCAGCAACA TACACCATCT CCTCCTGTGA TTCTGGGGAA 
AGCACCCTCA AGATCTGCAA CCTGATGCCC CAGGATAGTG GGATTTATAC CTGCATAGCA 
GCAAACGACC ACGGGACTGC ATCCACATCT GCTACAGTTA AGGTGCAAGG TGTCCCCGCA 
GCCCCCAACC GCCCAATCGC ACAGGAGAGG AGCTGCACCT CCGTCATCCT CCGCTGGCTG 
CCCCCAGCCA GCACCGGAAA CTGCACTATT TCGGGGTACA CAGTGGAGTA TCGCGAGGAA 
GGTTCCCAGG TCTGGCAGCA ATCCGTAGCT TCGACCTTGG ACACTTACCT GGTCATTGAA 
GACCTTAGTC CTGGGTGCCC TTATCAGTTC AGGGTCAGTG CCAGCAACCC CTGGGGAATC 
AGCCTTCCCA GTGAGCCCTC GGAGTTTGTG CGCCTTCCAG AGTACGATGC AGCCGCCGAC 
GGTGCCACCA TTTCTTGGAA GGAAAATTTT GATTCTGCTT ACACTGAACT GAACGAGATT 
GGCAGAGGCC GTTTCTCTAT TGTAAAGAAA TGCATTCACA AAGCTACCCG CAAAGATGTT 
GCTGTGAAAT TTGTGAGCAA AAAAATGAAG AAGAAGGAGC AGGCTGCTCA CGAGGCCGCC 
CTGCTCCAGC ACCTGCAGCA CCCTCAGTAT GTCACGCTCC ATGACACCTA CGAGTCCCCC 
ACATCCTACA TCCTGATTTT GGAACTGATG GATGACGGCC GACTCTTGGA CTACCTTATG 
AATCATGATG AGCTGATGGA AGAAAAGGTA GCTTTCTACA TTCGAGACAT CATGGAAGCT 
CTGCAGTACC TGCACAACTG TAGGGTCGCA CATTTGGACA TAAAGCCCGA AAACCTGCTC 
ATTGACCTCC GGATCCCAGT GCCTCGAGTG AAGCTCATCG ACTTGGAAGA CGCTGTCCAG 
ATCTCAGGCC ACTTCCATAT CCACCATCTG CTGGGGAACC CTGAGTTCGC TGCCCCCGAA 
GTGATCCAAG GCATCCCTGT CTCCCTGGGC ACAGACATCT GGAGCATCGG GGTTCTGACA 
TATGTCATGC TTAGTGGGGT CTCCCCCTTC TTAGATGAGA GCAAAGAGGA AACCTGTATC 
AACGTGTGCA GGGTGGACTT TAGCTTCCCC CACGAGTACT TCTGCGGTGT GAGCAATGCT 
GCCAGGGATT TCATCAACGT AATCTTACAG GAAGACTTCC GGAGGCGGCC CACAGCAGCC 
ACGTGCCTGC AGCATCCCTG GCTACAGCCC CACAACGGCA GCTACTCCAA GATCCCCTTG 
GACACCTCCC GTCTGGCCTG CTTCATAGAA CGCCGCAAGC ACCAGAATGA TGTGCGGCCC 
ATTCCCAATG TCAAGAGTTA CATCGTCAAT AGGGTGAACC AAGGGACCTC GCTATCCCAC 
AACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796331.2
CDS	48..8996
Translation	MTDRFWDQWYLWYLRLLRLLDRGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	NM_177357.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177357.3

ORF Insert Sequence:

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGAT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC TATCCTAAAG 
GAGAAAGTGG CCTTTGTGTC TGGGGGGCGT GACAAGCGAG GTGGACCCAT CCTGACCTTC 
CCTGCCCGAA GCAATCATGA CAGAATAAGA CAGGAAGACC TACGGAAACT TGTGACGTAT 
TTGGCCAGCG TGCCAAGTGA GGACGTTTGC AAACGTGGCT TCACCGTCAT CATCGACATG 
CGGGGCTCCA AGTGGGATCT CATCAAGCCC CTCCTCAAGA CGCTGCAGGA AGCCTTCCCC 
GCCGAGATCC ATGTGGCCCT CATCATCAAG CCTGACAACT TCTGGCAGAA GCAGAAGACC 
AACTTTGGCA GCTCCAAATT CATCTTTGAG ACAAGCATGG TGTCTGTGGA GGGCCTCACG 
AAGCTGGTGG ACCCCTCCCA GCTGACAGAG GAGTTTGACG GCTCCCTGGA TTACAACCAT 
GAGGAGTGGA TTGAGTTGCG CCTCTCCCTG GAGGAGTTCT TCAACAGTGC GGTCCACCTG 
CTCTCACGCC TTGAGGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC TGTGGATGTG 
GAGGGCTCTC GGAGGCTTAT CGACGAGCAC ACGCAACTTA AGAAAAAGGT GCTGAAGGCC 
CCCGTGGAAG AGCTGGACCG GGAGGGGCAG CGTCTACTGC AGTGCATCCG CTGCAGCGAT 
GGCTTCTCAG GGCGCAACTG CATCCCAGGA AGTGCCGACT TCCAGAGCCT TGTACCCAAG 
ATCACCAGCC TCCTGGACAA GCTGCATTCC ACCCGACAAC ACCTGCACCA AATGTGGCAC 
GTGCGAAAGC TCAAGCTGGA TCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAG 
AAGATGTTCG ACTGGATAAG CCACAACAAG GAGTTATTTC TTCAGAGCCA CACAGAGATC 
GGAGTCAGCT ACCAGCATGC ACTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC 
TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TCGCTTCCCG CCTCTCAGAG 
GCCGGCCATT ATGCCTCCCA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG 
AAGAGCTTTG CCGCTGCCCT GGATGAACGC AGTACAATCC TGGCCATGTC TGCTGTGTTC 
CACCAGAAGG CAGAGCAGTT CCTGTCAGGC GTGGATGCCT GGTGTAAGAT GTGCAGCGAA 
GGCGGCCTTC CTTCTGAGAT GCAGGACCTG GAGCTGGCAA TCCACCATCA CCAGTCCTTA 
TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ATGGCAAAGC ACTACTGGAT 
GTCCTGCAGC GTCCCCTGAG TCCTGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC 
TCCAAGGCGG TCCACCAGGT GCTGGATGTG GTCCACGAGG TGCTGCACCA TCAGCGACGG 
CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTTCACC AGCGGCTGCA GCTGTGTGTC 
TTCCAGCAGG ATGTGCAGCA GGTGCTGGAC TGGATTGAAA ACCATGGCGA GGCCTTTCTC 
AGCAAACACA CTGGGGTTGG CAAGTCCTTG CATCGAGCAC GTGCACTACA GAAGAGGCAT 
GATGACTTTG AAGAAGTGGC ACAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCA 
GCGGAGCAGC TGGCTCAGAC TGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCCCGG 
CACCTGGAGG TGCGCATCCA AGACTTTGTG CGCAGAGTGG AGCAGCGGAA GCTGCTTCTG 
GACATGTCGG TCTCTTTCCA CACGCACACC AAAGAGCTGT GGACATGGAT GGAGGACCTT 
CAGAAGGAGG TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAGCTGATC 
AAGCAGTTCC AGCAGCAGCA GACCGCCACC CTGGATGCCA CTCTCAATGT CATCAAGGAA 
GGCGAAGACC TTATCCAGCA GCTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCACCGAG 
GCCAGGGACT CAGCGATGTC CAACAACAAG ACACCCCACA GTAGCTCTAT CAGCCATATT 
GAGTCTGTCC TCCAGCAGCT GGACGATGCT CAGGTACAGA TGGAAGAGCT GTTTCACGAG 
CGGAAGATCA AGCTGGACAT CTTCCTACAG CTGCGCATCT TTGAACAGTA CACCATTGAG 
GTCACTGCAG AGCTGGATGC CTGGAACGAG GACTTGCTCC GGCAGATGAA TGATTTCAAC 
ACAGAGGATC TGACCCTGGC TGAGCAGCGG CTACAGCGCC ACACAGAACG GAAGCTTGCC 
ATGAACAACA TGACGTTCGA GGTCATCCAG CAGGGCCAGG ACCTGCACCA GTACATCATG 
GAAGTGCAAG CGTCAGGAAT CGAGTTAATC TGTGAGAAGG ACCTTGACCT GGCAGCTCAG 
GTGCAGGAGC TGCTGGAGTT TCTCCATGAG AAGCAGCACG AGCTGGAGCT CAATGCGGAG 
CAGACGCACA AGCGGCTAGA GCAGTGTCTC CAGCTGAGGC ACCTCCAGGC TGAGGTCAAA 
CAGGTTCTGG GATGGATCCG CAATGGAGAG TCGATGCTCA ACGCCAGCCT GGTCAACGCC 
AGCTCTTTGT CGGAGGCAGA GCAGCTGCAG CGGGAGCATG AACAGTTCCA GCTAGCCATC 
GAGAAGACGC ACCAGAGCGC CCTGCAGGTA CAGCAGAAGG CAGAGGCACT GCTCCAGGCC 
GGCCACTACG ACGCAGACGC CATCCGGGAA TGTGCTGAGA AGGTGGCCCT CCACTGGCAA 
CAGCTCATGC TAAAGATGGA GGACCGGCTG AAGCTTGTCA ACGCCTCTGT GGCCTTTTAC 
AAGACCTCTG AGCAGGTGTG CAGTGTTCTG GAGAGCTTGG AGCAGGAGTA CCGGAGAGAC 
GAGGACTGGT GTGGTGGGAG AGACAAGCTG GGGCCGGCGG CAGAGATGGA CCACGTTATT 
CCACTCCTCA GCAAGCACCT GGAGCAGAAG GAGGCCTTCC TGAAGGCCTG CACGCTGGCA 
AGGCGGAACG CCGAGGTGTT CCTCAAATAC ATCCACAGGA ACAATGTGAG CATGCCTAGT 
GTTGCCAGCC ACACCCGGGG GCCTGAACAG CAAGTAAAAG CCATCTTGAG CGAGCTCTTG 
CAGAGGGAGA ATCGAGTCCT GCACTTCTGG ACCTTGAAGA AGCGGCGGTT AGACCAGTGT 
CAGCAATATG TGGTGTTTGA GCGCAGTGCT AAGCAGGCAC TGGACTGGAT CCAAGAAACA 
GGAGAATATT ACCTCTCAAC ACATACCTCC ACCGGAGAGA CCACGGAGGA GACTCAGGAG 
CTGCTGAAAG AGTATGGAGA ATTCAGGGTG CCTGCCAAGC AAACGAAGGA GAAGGTGAAA 
CTTCTGATCC AGCTAGCAGA CAGCTTTGTG GAAAAGGGCC ACATCCACGC CACAGAGATA 
CGGAAATGGG TGACCACGGT GGACAAGCAC TACCGCGATT TCTCCCTGAG GATGGGGAAG 
TACCGGTACT CGCTGGAGAA GGCGCTGGGG GTCAACACGG AGGATAACAA GGACCTAGAG 
CTGGACATCA TCCCAGCAAG CCTTTCGGAT CGGGAGGTCA AGCTCCGGGA CGCTAACCAT 
GAGATCAATG AAGAGAAACG GAAGTCAGCT CGAAAGAAAG AGTTCATTAT GGCTGAACTG 
CTCCAAACTG AGAAGGCTTA TGTAAGGGAC TTGCACGAGT GCTTGGAGAC CTACCTGTGG 
GAGATGACTA GTGGTGTGGA AGAGATACCC CCCGGCATCC TCAATAAGGA ACATATCATC 
TTCGGCAACA TCCAGGAGAT CTATGACTTC CATAACAACA TCTTCCTCAA AGAGCTGGAG 
AAGTACGAGC AGCTGCCTGA GGATGTGGGA CACTGCTTTG TTACCTGGGC AGACAAATTT 
CAGATGTATG TCACCTACTG TAAAAACAAG CCTGATTCCA ACCAGCTGAT CCTGGAGCAT 
GCGGGCACCT TCTTTGATGA GATCCAGCAG CGGCATGGCC TGGCCAACTC CATCTCCTCC 
TACCTAATTA AGCCTGTCCA GAGGGTTACC AAGTACCAGC TGCTCCTGAA GGAACTTCTA 
ACTTGCTGTG AAGAAGGGAA AGGAGAGCTG AAGGATGGCC TGGAGGTGAT GCTCAGTGTC 
CCCAAGAAAG CCAACGATGC CATGCATGTC AGCATGCTGG AAGGGTTCGA TGAAAACTTG 
GATGTACAGG GGGAGTTGAT TCTCCAGGAT GCCTTTCAAG TGTGGGACCC AAAGTCGCTG 
ATCCGGAAGG GGCGGGAGCG CCATCTGTTC CTCTTTGAGA TCTCCTTGGT GTTTAGCAAA 
GAGATCAAAG ACTCTTCAGG ACACACGAAA TATGTTTACA AGAACAAGCT ACTGACCTCA 
GAGCTGGGTG TGACCGAGCA TGTAGAAGGT GACCCCTGCA AATTCGCCTT GTGGTCTGGG 
CGCACCCCAT CCTCAGACAA TAAAACAGTG CTCAAAGCTT CCAACATCGA AACCAAGCAG 
GAATGGATCA AGAACATTCG AGAAGTGATT CAGGAGAGGA TCATTCATCT TAAAGGCGCT 
TTAAAGGAGC CAATTCAGCT CCCCAAAACA CCAGCCAAAC TGAGGAACAA TAGTAAGAGG 
GATGGGGTAG AGGATGGTGA CAGCCAAGGG GATGGGAGCA GCCAGCCAGA TACCATATCC 
ATTGCCTCCA GGACCTCTCA GAACACAGTG GAAAGTGACA AGCTCTCAGG TGGATGTGAG 
CTGACCGTGG TCCTCCAGGA CTTCAGCGCA GGCCACAGCA GCGAGCTGAG CATCCAGGTG 
GGCCAGACGG TGGAGCTGCT GGAACGACCG AGTGAGAGGC CTGGCTGGTG TCTGGTCCGC 
ACCACAGAGC GGAGCCCCCC ACAGGAAGGC CTGGTACCCA GCAGCGCCCT GTGCATCTCA 
CACTCTCGCA GCAGCGTGGA AATGGACTGC TTCTTCCCCT TGAAAGATTC ATACTCTCAT 
TCCTCCAGTG AGAATGGAGG CAAATCCGAG TCCGTGGCCC ACCTGCAGTC TCAGCCCTCC 
CTGAACTCCA TCCACAGTTC CCCTGGTCCC AAGCGCTCCA CCAACACCCT CAAAAAGTGG 
CTGACCAGCC CCGTGCGCCG CCTCAACAGC GGAAAAGCCG ACGGGAACAT CAAAAAGCAG 
AAGAAAGTCC GGGATGGGCG GAAGAGCTTC GATCTGGGAT CGCCCAAGCC TGGGGATGAG 
ACGACACCTC AGGGAGACAG CGCTGACGAG AAGAGCAAGA AGGGTTGGGG CGAAGACGAG 
CCAGATGAAG AGTCACACAC ACCCCTCCCT CCGCCCATGA AGATCTTTGA CAACGACCCC 
ACACAGGATG AGATGTCGTC TTTGCTAGCA GCCCGGCAGG CTCCCCCTGA CGTGCCTACT 
GCTGCAGACC TTGTCAGTGC AATAGAAAAG TTGGTCAAAA ATAAGCTGAC TCTGGAAGGA 
GGCTCTTACC GGGGGAGCTT GAAGGACCCT ACAGGCTGCC TGAATGAGGG GATGACCCCT 
CCCACACCTC CTAGAAACCT GGAAGAAGAA CAGAAAGCCA AGGCGCTGAG AGGCAGGATG 
TTTGTCCTGA ATGAGCTGGT ACAGACAGAG AAGGACTACG TCAAGGACCT GGGGATTGTG 
GTGGAGGGCT TCATGAAGAG AATAGAAGAG AAGGGGGTCC CTGAGGACAT GCGAGGGAAG 
GAAAAGATCG TGTTTGGAAA TATCCACCAG ATTTACGACT GGCATAAAGA TTTTTTCCTG 
GCTGAACTGG AAAAATGTAT CCAAGAGCAA GACAGATTGG CCCAGCTCTT CATTAAACAC 
GAGCGGAAGC TGCACATCTA CGTGTGGTAC TGCCAGAACA AGCCGCGTTC CGAGTACATC 
GTGGCTGAGT ACGATGCCTA TTTTGAGGAG GTTAAACAAG AGATCAATCA AAGGCTGACC 
CTTAGTGACT TCCTGATCAA GCCCATTCAG AGAATAACAA AGTACCAGCT GCTACTCAAG 
GACTTCCTGC GATACAGCGA GAAGGCTGGT TTGGAGTGTT CCGATATTGA GAAAGCAGTG 
GAGTTAATGT GCCTTGTCCC CAAGCGCTGT AATGACATGA TGAATCTGGG ACGCCTGCAG 
GGCTTCGAGG GCACCCTGAC CGCCCAGGGC AAGCTACTGC AGCAGGACAC ATTCTATGTG 
ATCGAGCTGG ATGCCGGCAT GCAGTCCCGG ACCAAGGAGA GGCGTGTGTT TCTCTTTGAG 
CAGATTGTCA TCTTCAGTGA ACTGCTCCGA AAGGGGTCCC TCACCCCAGG CTACATGTTC 
AAAAGAAGCA TCAAGATGAA TTACTTGGTC CTGGAAGACA ATGTGGATGG TGACCCCTGC 
AAATTTGCAC TCATGAACAG GGAGACTTCA GAAAGGGTCA TTCTACAGGC TGCCAACTCA 
GACATCCAGC AGGCCTGGGT GCAGGACATC AACCAAGTCC TAGAAACACA GAGGGACTTC 
TTAAATGCAC TACAGTCACC CATTGAGTAT CAGCGGAAGG AGAGAAGCAC AGCTGTGATC 
CGGTCCCAGC CCCCGAGGGT TCCACAAGCC AGCCCCAGGC CCTACTCTTC TGGCCCGGTG 
GGCTCAGAGA AGCCCCCCAA GGGCTCCAGC TATAACCCGC CTCTGCCACC CCTGAAGATA 
TCTACCTCTA ATGGCAGTCC AGGGTTCGAC TACCATCAGC CTGGGGACAA GTTTGACGCT 
AGCAAGCAGA ACGACCTGGG AGGCTGCAAT GGGACATCAA CCATGACTGT AATCAAAGAT 
TACTATGCAC TGAAGGAGAA TGAAATCTGC GTGAGCCAAG GTGAGGTGGT ACAGGTCCTC 
GCCGTCAACC AGCAGAACAT GTGTCTGGTG TACCAGCCTG CCAGCGACCA TTCCCCGGCA 
GCAGAGGGCT GGGTCCCAGG CAGCATCCTG GCGCCCCTCG CCAAAGCCAC AGCAGCGGCC 
GAAAGTAGTG ACGGGAGCAT CAAGAAGTCA TGCTCTTGGC ACACTCTACG CATGAGAAAG 
CGGGCAGACG TGGAGAACTC GGGTAAAAAT GAAGCCACAG GGCCTCGTAA ACCCAAGGAT 
ATTCTGGGCA ACAAAGTCTC TGTTAAAGAG ACGAACAGTT CCGAGGAGTC AGAGTGTGAT 
GATCTTGACC CTAATACTAG TATGGAGATC TTGAATCCAA ATTTCATCCA GGAAGTGGCT 
CCAGAATTCC TCGTGCCCTT AGTAGATGTG ACCTGTTTGC TTGGAGACAC AGTGCTACTG 
CAGTGTAAAG CCTGTGGGCG GCCAAAGCCC AGCATCACCT GGAAGGGTCC GGATCAGAAC 
ATCCTAGACA CTGACAACAG TTCAGCAACA TACACCATCT CCTCCTGTGA TTCTGGGGAA 
AGCACCCTCA AGATCTGCAA CCTGATGCCC CAGGATAGTG GGATTTATAC CTGCATAGCA 
GCAAACGACC ACGGGACTGC ATCCACATCT GCTACAGTTA AGGTGCAAGG TGTCCCCGCA 
GCCCCCAACC GCCCAATCGC ACAGGAGAGG AGCTGCACCT CCGTCATCCT CCGCTGGCTG 
CCCCCAGCCA GCACCGGAAA CTGCACTATT TCGGGGTACA CAGTGGAGTA TCGCGAGGAA 
GGTTCCCAGG TCTGGCAGCA ATCCGTAGCT TCGACCTTGG ACACTTACCT GGTCATTGAA 
GACCTTAGTC CTGGGTGCCC TTATCAGTTC AGGGTCAGTG CCAGCAACCC CTGGGGAATC 
AGCCTTCCCA GTGAGCCCTC GGAGTTTGTG CGCCTTCCAG AGTACGATGC AGCCGCCGAC 
GGTGCCACCA TTTCTTGGAA GGAAAATTTT GATTCTGCTT ACACTGAACT GAACGAGATT 
GGCAGAGGCC GTTTCTCTAT TGTAAAGAAA TGCATTCACA AAGCTACCCG CAAAGATGTT 
GCTGTGAAAT TTGTGAGCAA AAAAATGAAG AAGAAGGAGC AGGCTGCTCA CGAGGCCGCC 
CTGCTCCAGC ACCTGCAGCA CCCTCAGTAT GTCACGCTCC ATGACACCTA CGAGTCCCCC 
ACATCCTACA TCCTGATTTT GGAACTGATG GATGACGGCC GACTCTTGGA CTACCTTATG 
AATCATGATG AGCTGATGGA AGAAAAGGTA GCTTTCTACA TTCGAGACAT CATGGAAGCT 
CTGCAGTACC TGCACAACTG TAGGGTCGCA CATTTGGACA TAAAGCCCGA AAACCTGCTC 
ATTGACCTCC GGATCCCAGT GCCTCGAGTG AAGCTCATCG ACTTGGAAGA CGCTGTCCAG 
ATCTCAGGCC ACTTCCATAT CCACCATCTG CTGGGGAACC CTGAGTTCGC TGCCCCCGAA 
GTGATCCAAG GCATCCCTGT CTCCCTGGGC ACAGACATCT GGAGCATCGG GGTTCTGACA 
TATGTCATGC TTAGTGGGGT CTCCCCCTTC TTAGATGAGA GCAAAGAGGA AACCTGTATC 
AACGTGTGCA GGGTGGACTT TAGCTTCCCC CACGAGTACT TCTGCGGTGT GAGCAATGCT 
GCCAGGGATT TCATCAACGT AATCTTACAG GAAGACTTCC GGAGGCGGCC CACAGCAGCC 
ACGTGCCTGC AGCATCCCTG GCTACAGCCC CACAACGGCA GCTACTCCAA GATCCCCTTG 
GACACCTCCC GTCTGGCCTG CTTCATAGAA CGCCGCAAGC ACCAGAATGA TGTGCGGCCC 
ATTCCCAATG TCAAGAGTTA CATCGTCAAT AGGGTGAACC AAGGGACCTC GCTATCCCAC 
AACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522363.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522426.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X6, mRNA.

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CloneID	OMu52279
Clone ID Related Accession (Same CDS sequence)	XM_006522363.3 , XM_006522363.4
Accession Version	XM_006522363.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522363.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGAT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC TATCCTAAAG 
GAGAAAGTGG CCTTTGTGTC TGGGGGGCGT GACAAGCGAG GTGGACCCAT CCTGACCTTC 
CCTGCCCGAA GCAATCATGA CAGAATAAGA CAGGAAGACC TACGGAAACT TGTGACGTAT 
TTGGCCAGCG TGCCAAGTGA GGACGTTTGC AAACGTGGCT TCACCGTCAT CATCGACATG 
CGGGGCTCCA AGTGGGATCT CATCAAGCCC CTCCTCAAGA CGCTGCAGGA AGCCTTCCCC 
GCCGAGATCC ATGTGGCCCT CATCATCAAG CCTGACAACT TCTGGCAGAA GCAGAAGACC 
AACTTTGGCA GCTCCAAATT CATCTTTGAG ACAAGCATGG TGTCTGTGGA GGGCCTCACG 
AAGCTGGTGG ACCCCTCCCA GCTGACAGAG GAGTTTGACG GCTCCCTGGA TTACAACCAT 
GAGGAGTGGA TTGAGTTGCG CCTCTCCCTG GAGGAGTTCT TCAACAGTGC GGTCCACCTG 
CTCTCACGCC TTGAGGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC TGTGGATGTG 
GAGGGCTCTC GGAGGCTTAT CGACGAGCAC ACGCAACTTA AGAAAAAGGT GCTGAAGGCC 
CCCGTGGAAG AGCTGGACCG GGAGGGGCAG CGTCTACTGC AGTGCATCCG CTGCAGCGAT 
GGCTTCTCAG GGCGCAACTG CATCCCAGGA AGTGCCGACT TCCAGAGCCT TGTACCCAAG 
ATCACCAGCC TCCTGGACAA GCTGCATTCC ACCCGACAAC ACCTGCACCA AATGTGGCAC 
GTGCGAAAGC TCAAGCTGGA TCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAG 
AAGATGTTCG ACTGGATAAG CCACAACAAG GAGTTATTTC TTCAGAGCCA CACAGAGATC 
GGAGTCAGCT ACCAGCATGC ACTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC 
TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TCGCTTCCCG CCTCTCAGAG 
GCCGGCCATT ATGCCTCCCA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG 
AAGAGCTTTG CCGCTGCCCT GGATGAACGC AGTACAATCC TGGCCATGTC TGCTGTGTTC 
CACCAGAAGG CAGAGCAGTT CCTGTCAGGC GTGGATGCCT GGTGTAAGAT GTGCAGCGAA 
GGCGGCCTTC CTTCTGAGAT GCAGGACCTG GAGCTGGCAA TCCACCATCA CCAGTCCTTA 
TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ATGGCAAAGC ACTACTGGAT 
GTCCTGCAGC GTCCCCTGAG TCCTGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC 
TCCAAGGCGG TCCACCAGGT GCTGGATGTG GTCCACGAGG TGCTGCACCA TCAGCGACGG 
CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTTCACC AGCGGCTGCA GCTGTGTGTC 
TTCCAGCAGG ATGTGCAGCA GGTGCTGGAC TGGATTGAAA ACCATGGCGA GGCCTTTCTC 
AGCAAACACA CTGGGGTTGG CAAGTCCTTG CATCGAGCAC GTGCACTACA GAAGAGGCAT 
GATGACTTTG AAGAAGTGGC ACAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCA 
GCGGAGCAGC TGGCTCAGAC TGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCCCGG 
CACCTGGAGG TGCGCATCCA AGACTTTGTG CGCAGAGTGG AGCAGCGGAA GCTGCTTCTG 
GACATGTCGG TCTCTTTCCA CACGCACACC AAAGAGCTGT GGACATGGAT GGAGGACCTT 
CAGAAGGAGG TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAGCTGATC 
AAGCAGTTCC AGCAGCAGCA GACCGCCACC CTGGATGCCA CTCTCAATGT CATCAAGGAA 
GGCGAAGACC TTATCCAGCA GCTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCACCGAG 
GCCAGGGACT CAGCGATGTC CAACAACAAG ACACCCCACA GTAGCTCTAT CAGCCATATT 
GAGTCTGTCC TCCAGCAGCT GGACGATGCT CAGGTACAGA TGGAAGAGCT GTTTCACGAG 
CGGAAGATCA AGCTGGACAT CTTCCTACAG CTGCGCATCT TTGAACAGTA CACCATTGAG 
GTCACTGCAG AGCTGGATGC CTGGAACGAG GACTTGCTCC GGCAGATGAA TGATTTCAAC 
ACAGAGGATC TGACCCTGGC TGAGCAGCGG CTACAGCGCC ACACAGAACG GAAGCTTGCC 
ATGAACAACA TGACGTTCGA GGTCATCCAG CAGGGCCAGG ACCTGCACCA GTACATCATG 
GAAGTGCAAG CGTCAGGAAT CGAGTTAATC TGTGAGAAGG ACCTTGACCT GGCAGCTCAG 
GTGCAGGAGC TGCTGGAGTT TCTCCATGAG AAGCAGCACG AGCTGGAGCT CAATGCGGAG 
CAGACGCACA AGCGGCTAGA GCAGTGTCTC CAGCTGAGGC ACCTCCAGGC TGAGGTCAAA 
CAGGTTCTGG GATGGATCCG CAATGGAGAG TCGATGCTCA ACGCCAGCCT GGTCAACGCC 
AGCTCTTTGT CGGAGGCAGA GCAGCTGCAG CGGGAGCATG AACAGTTCCA GCTAGCCATC 
GAGTCCCTCT TTCATGCCAC TTCCTTGCAG AAGACGCACC AGAGCGCCCT GCAGGTACAG 
CAGAAGGCAG AGGCACTGCT CCAGGCCGGC CACTACGACG CAGACGCCAT CCGGGAATGT 
GCTGAGAAGG TGGCCCTCCA CTGGCAACAG CTCATGCTAA AGATGGAGGA CCGGCTGAAG 
CTTGTCAACG CCTCTGTGGC CTTTTACAAG ACCTCTGAGC AGGTGTGCAG TGTTCTGGAG 
AGCTTGGAGC AGGAGTACCG GAGAGACGAG GACTGGTGTG GTGGGAGAGA CAAGCTGGGG 
CCGGCGGCAG AGATGGACCA CGTTATTCCA CTCCTCAGCA AGCACCTGGA GCAGAAGGAG 
GCCTTCCTGA AGGCCTGCAC GCTGGCAAGG CGGAACGCCG AGGTGTTCCT CAAATACATC 
CACAGGAACA ATGTGAGCAT GCCTAGTGTT GCCAGCCACA CCCGGGGGCC TGAACAGCAA 
GTAAAAGCCA TCTTGAGCGA GCTCTTGCAG AGGGAGAATC GAGTCCTGCA CTTCTGGACC 
TTGAAGAAGC GGCGGTTAGA CCAGTGTCAG CAATATGTGG TGTTTGAGCG CAGTGCTAAG 
CAGGCACTGG ACTGGATCCA AGAAACAGGA GAATATTACC TCTCAACACA TACCTCCACC 
GGAGAGACCA CGGAGGAGAC TCAGGAGCTG CTGAAAGAGT ATGGAGAATT CAGGGTGCCT 
GCCAAGCAAA CGAAGGAGAA GGTGAAACTT CTGATCCAGC TAGCAGACAG CTTTGTGGAA 
AAGGGCCACA TCCACGCCAC AGAGATACGG AAATGGGTGA CCACGGTGGA CAAGCACTAC 
CGCGATTTCT CCCTGAGGAT GGGGAAGTAC CGGTACTCGC TGGAGAAGGC GCTGGGGGTC 
AACACGGAGG ATAACAAGGA CCTAGAGCTG GACATCATCC CAGCAAGCCT TTCGGATCGG 
GAGGTCAAGC TCCGGGACGC TAACCATGAG ATCAATGAAG AGAAACGGAA GTCAGCTCGA 
AAGAAAGAGT TCATTATGGC TGAACTGCTC CAAACTGAGA AGGCTTATGT AAGGGACTTG 
CACGAGTGCT TGGAGACCTA CCTGTGGGAG ATGACTAGTG GTGTGGAAGA GATACCCCCC 
GGCATCCTCA ATAAGGAACA TATCATCTTC GGCAACATCC AGGAGATCTA TGACTTCCAT 
AACAACATCT TCCTCAAAGA GCTGGAGAAG TACGAGCAGC TGCCTGAGGA TGTGGGACAC 
TGCTTTGTTA CCTGGGCAGA CAAATTTCAG ATGTATGTCA CCTACTGTAA AAACAAGCCT 
GATTCCAACC AGCTGATCCT GGAGCATGCG GGCACCTTCT TTGATGAGAT CCAGCAGCGG 
CATGGCCTGG CCAACTCCAT CTCCTCCTAC CTAATTAAGC CTGTCCAGAG GGTTACCAAG 
TACCAGCTGC TCCTGAAGGA ACTTCTAACT TGCTGTGAAG AAGGGAAAGG AGAGCTGAAG 
GATGGCCTGG AGGTGATGCT CAGTGTCCCC AAGAAAGCCA ACGATGCCAT GCATGTCAGC 
ATGCTGGAAG GGTTCGATGA AAACTTGGAT GTACAGGGGG AGTTGATTCT CCAGGATGCC 
TTTCAAGTGT GGGACCCAAA GTCGCTGATC CGGAAGGGGC GGGAGCGCCA TCTGTTCCTC 
TTTGAGATCT CCTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACGAAATAT 
GTTTACAAGA ACAAGCTACT GACCTCAGAG CTGGGTGTGA CCGAGCATGT AGAAGGTGAC 
CCCTGCAAAT TCGCCTTGTG GTCTGGGCGC ACCCCATCCT CAGACAATAA AACAGTGCTC 
AAAGCTTCCA ACATCGAAAC CAAGCAGGAA TGGATCAAGA ACATTCGAGA AGTGATTCAG 
GAGAGGATCA TTCATCTTAA AGGCGCTTTA AAGGAGCCAA TTCAGCTCCC CAAAACACCA 
GCCAAACTGA GGAACAATAG TAAGAGGGAT GGGGTAGAGG ATGGTGACAG CCAAGGGGAT 
GGGAGCAGCC AGCCAGATAC CATATCCATT GCCTCCAGGA CCTCTCAGAA CACAGTGGAA 
AGTGACAAGC TCTCAGGTGG ATGTGAGCTG ACCGTGGTCC TCCAGGACTT CAGCGCAGGC 
CACAGCAGCG AGCTGAGCAT CCAGGTGGGC CAGACGGTGG AGCTGCTGGA ACGACCGAGT 
GAGAGGCCTG GCTGGTGTCT GGTCCGCACC ACAGAGCGGA GCCCCCCACA GGAAGGCCTG 
GTACCCAGCA GCGCCCTGTG CATCTCACAC TCTCGCAGCA GCGTGGAAAT GGACTGCTTC 
TTCCCCTTGA AAGATTCATA CTCTCATTCC TCCAGTGAGA ATGGAGGCAA ATCCGAGTCC 
GTGGCCCACC TGCAGTCTCA GCCCTCCCTG AACTCCATCC ACAGTTCCCC TGGTCCCAAG 
CGCTCCACCA ACACCCTCAA AAAGTGGCTG ACCAGCCCCG TGCGCCGCCT CAACAGCGGA 
AAAGCCGACG GGAACATCAA AAAGCAGAAG AAAGTCCGGG ATGGGCGGAA GAGCTTCGAT 
CTGGGATCGC CCAAGCCTGG GGATGAGACG ACACCTCAGG GAGACAGCGC TGACGAGAAG 
AGCAAGAAGG GTTGGGGCGA AGACGAGCCA GATGAAGAGT CACACACACC CCTCCCTCCG 
CCCATGAAGA TCTTTGACAA CGACCCCACA CAGGATGAGA TGTCGTCTTT GCTAGCAGCC 
CGGCAGGCTC CCCCTGACGT GCCTACTGCT GCAGACCTTG TCAGTGCAAT AGAAAAGTTG 
GTCAAAAATA AGCTGACTCT GGAAGGAGGC TCTTACCGGG GGAGCTTGAA GGACCCTACA 
GGCTGCCTGA ATGAGGGGAT GACCCCTCCC ACACCTCCTA GAAACCTGGA AGAAGAACAG 
AAAGCCAAGG CGCTGAGAGG CAGGATGTTT GTCCTGAATG AGCTGGTACA GACAGAGAAG 
GACTACGTCA AGGACCTGGG GATTGTGGTG GAGGGCTTCA TGAAGAGAAT AGAAGAGAAG 
GGGGTCCCTG AGGACATGCG AGGGAAGGAA AAGATCGTGT TTGGAAATAT CCACCAGATT 
TACGACTGGC ATAAAGATTT TTTCCTGGCT GAACTGGAAA AATGTATCCA AGAGCAAGAC 
AGATTGGCCC AGCTCTTCAT TAAACACGAG CGGAAGCTGC ACATCTACGT GTGGTACTGC 
CAGAACAAGC CGCGTTCCGA GTACATCGTG GCTGAGTACG ATGCCTATTT TGAGGAGGTT 
AAACAAGAGA TCAATCAAAG GCTGACCCTT AGTGACTTCC TGATCAAGCC CATTCAGAGA 
ATAACAAAGT ACCAGCTGCT ACTCAAGGAC TTCCTGCGAT ACAGCGAGAA GGCTGGTTTG 
GAGTGTTCCG ATATTGAGAA AGCAGTGGAG TTAATGTGCC TTGTCCCCAA GCGCTGTAAT 
GACATGATGA ATCTGGGACG CCTGCAGGGC TTCGAGGGCA CCCTGACCGC CCAGGGCAAG 
CTACTGCAGC AGGACACATT CTATGTGATC GAGCTGGATG CCGGCATGCA GTCCCGGACC 
AAGGAGAGGC GTGTGTTTCT CTTTGAGCAG ATTGTCATCT TCAGTGAACT GCTCCGAAAG 
GGGTCCCTCA CCCCAGGCTA CATGTTCAAA AGAAGCATCA AGATGAATTA CTTGGTCCTG 
GAAGACAATG TGGATGGTGA CCCCTGCAAA TTTGCACTCA TGAACAGGGA GACTTCAGAA 
AGGGTCATTC TACAGGCTGC CAACTCAGAC ATCCAGCAGG CCTGGGTGCA GGACATCAAC 
CAAGTCCTAG AAACACAGAG GGACTTCTTA AATGCACTAC AGTCACCCAT TGAGTATCAG 
CGGAAGGAGA GAAGCACAGC TGTGATCCGG TCCCAGCCCC CGAGGGTTCC ACAAGCCAGC 
CCCAGGCCCT ACTCTTCTGG CCCGGTGGGC TCAGAGAAGC CCCCCAAGGG CTCCAGCTAT 
AACCCGCCTC TGCCACCCCT GAAGATATCT ACCTCTAATG GCAGTCCAGG GTTCGACTAC 
CATCAGCCTG GGGACAAGTT TGACGCTAGC AAGCAGAACG ACCTGGGAGG CTGCAATGGG 
ACATCAACCA TGACTGTAAT CAAAGATTAC TATGCACTGA AGGAGAATGA AATCTGCGTG 
AGCCAAGGTG AGGTGGTACA GGTCCTCGCC GTCAACCAGC AGAACATGTG TCTGGTGTAC 
CAGCCTGCCA GCGACCATTC CCCGGCAGCA GAGGGCTGGG TCCCAGGCAG CATCCTGGCG 
CCCCTCGCCA AAGCCACAGC AGCGGCCGAA AGTAGTGACG GGAGCATCAA GAAGTCATGC 
TCTTGGCACA CTCTACGCAT GAGAAAGCGG GCAGACGTGG AGAACTCGGG TAAAAATGAA 
GCCACAGGGC CTCGTAAACC CAAGGATATT CTGGGCAACA AAGTCTCTGT TAAAGAGACG 
AACAGTTCCG AGGAGTCAGA GTGTGATGAT CTTGACCCTA ATACTAGTAT GGAGATCTTG 
AATCCAAATT TCATCCAGGA AGTGGCTCCA GAATTCCTCG TGCCCTTAGT AGATGTGACC 
TGTTTGCTTG GAGACACAGT GCTACTGCAG TGTAAAGCCT GTGGGCGGCC AAAGCCCAGC 
ATCACCTGGA AGGGTCCGGA TCAGAACATC CTAGACACTG ACAACAGTTC AGCAACATAC 
ACCATCTCCT CCTGTGATTC TGGGGAAAGC ACCCTCAAGA TCTGCAACCT GATGCCCCAG 
GATAGTGGGA TTTATACCTG CATAGCAGCA AACGACCACG GGACTGCATC CACATCTGCT 
ACAGTTAAGG TGCAAGGTGT CCCCGCAGCC CCCAACCGCC CAATCGCACA GGAGAGGAGC 
TGCACCTCCG TCATCCTCCG CTGGCTGCCC CCAGCCAGCA CCGGAAACTG CACTATTTCG 
GGGTACACAG TGGAGTATCG CGAGGAAGGT TCCCAGGTCT GGCAGCAATC CGTAGCTTCG 
ACCTTGGACA CTTACCTGGT CATTGAAGAC CTTAGTCCTG GGTGCCCTTA TCAGTTCAGG 
GTCAGTGCCA GCAACCCCTG GGGAATCAGC CTTCCCAGTG AGCCCTCGGA GTTTGTGCGC 
CTTCCAGAGT ACGATGCAGC CGCCGACGGT GCCACCATTT CTTGGAAGGA AAATTTTGAT 
TCTGCTTACA CTGAACTGAA CGAGATTGGC AGAGGCCGTT TCTCTATTGT AAAGAAATGC 
ATTCACAAAG CTACCCGCAA AGATGTTGCT GTGAAATTTG TGAGCAAAAA AATGAAGAAG 
AAGGAGCAGG CTGCTCACGA GGCCGCCCTG CTCCAGCACC TGCAGCACCC TCAGTATGTC 
ACGCTCCATG ACACCTACGA GTCCCCCACA TCCTACATCC TGATTTTGGA ACTGATGGAT 
GACGGCCGAC TCTTGGACTA CCTTATGAAT CATGATGAGC TGATGGAAGA AAAGGTAGCT 
TTCTACATTC GAGACATCAT GGAAGCTCTG CAGTACCTGC ACAACTGTAG GGTCGCACAT 
TTGGACATAA AGCCCGAAAA CCTGCTCATT GACCTCCGGA TCCCAGTGCC TCGAGTGAAG 
CTCATCGACT TGGAAGACGC TGTCCAGATC TCAGGCCACT TCCATATCCA CCATCTGCTG 
GGGAACCCTG AGTTCGCTGC CCCCGAAGTG ATCCAAGGCA TCCCTGTCTC CCTGGGCACA 
GACATCTGGA GCATCGGGGT TCTGACATAT GTCATGCTTA GTGGGGTCTC CCCCTTCTTA 
GATGAGAGCA AAGAGGAAAC CTGTATCAAC GTGTGCAGGG TGGACTTTAG CTTCCCCCAC 
GAGTACTTCT GCGGTGTGAG CAATGCTGCC AGGGATTTCA TCAACGTAAT CTTACAGGAA 
GACTTCCGGA GGCGGCCCAC AGCAGCCACG TGCCTGCAGC ATCCCTGGCT ACAGCCCCAC 
AACGGCAGCT ACTCCAAGAT CCCCTTGGAC ACCTCCCGTC TGGCCTGCTT CATAGAACGC 
CGCAAGCACC AGAATGATGT GCGGCCCATT CCCAATGTCA AGAGTTACAT CGTCAATAGG 
GTGAACCAAG GGACCTCGCT ATCCCACAAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522426.1
CDS	3335..12310
Translation	MTDRFWDQWYLWYLRLLRLLDRGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_006522363.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522363.3

ORF Insert Sequence:

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGAT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC TATCCTAAAG 
GAGAAAGTGG CCTTTGTGTC TGGGGGGCGT GACAAGCGAG GTGGACCCAT CCTGACCTTC 
CCTGCCCGAA GCAATCATGA CAGAATAAGA CAGGAAGACC TACGGAAACT TGTGACGTAT 
TTGGCCAGCG TGCCAAGTGA GGACGTTTGC AAACGTGGCT TCACCGTCAT CATCGACATG 
CGGGGCTCCA AGTGGGATCT CATCAAGCCC CTCCTCAAGA CGCTGCAGGA AGCCTTCCCC 
GCCGAGATCC ATGTGGCCCT CATCATCAAG CCTGACAACT TCTGGCAGAA GCAGAAGACC 
AACTTTGGCA GCTCCAAATT CATCTTTGAG ACAAGCATGG TGTCTGTGGA GGGCCTCACG 
AAGCTGGTGG ACCCCTCCCA GCTGACAGAG GAGTTTGACG GCTCCCTGGA TTACAACCAT 
GAGGAGTGGA TTGAGTTGCG CCTCTCCCTG GAGGAGTTCT TCAACAGTGC GGTCCACCTG 
CTCTCACGCC TTGAGGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC TGTGGATGTG 
GAGGGCTCTC GGAGGCTTAT CGACGAGCAC ACGCAACTTA AGAAAAAGGT GCTGAAGGCC 
CCCGTGGAAG AGCTGGACCG GGAGGGGCAG CGTCTACTGC AGTGCATCCG CTGCAGCGAT 
GGCTTCTCAG GGCGCAACTG CATCCCAGGA AGTGCCGACT TCCAGAGCCT TGTACCCAAG 
ATCACCAGCC TCCTGGACAA GCTGCATTCC ACCCGACAAC ACCTGCACCA AATGTGGCAC 
GTGCGAAAGC TCAAGCTGGA TCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAG 
AAGATGTTCG ACTGGATAAG CCACAACAAG GAGTTATTTC TTCAGAGCCA CACAGAGATC 
GGAGTCAGCT ACCAGCATGC ACTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC 
TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TCGCTTCCCG CCTCTCAGAG 
GCCGGCCATT ATGCCTCCCA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG 
AAGAGCTTTG CCGCTGCCCT GGATGAACGC AGTACAATCC TGGCCATGTC TGCTGTGTTC 
CACCAGAAGG CAGAGCAGTT CCTGTCAGGC GTGGATGCCT GGTGTAAGAT GTGCAGCGAA 
GGCGGCCTTC CTTCTGAGAT GCAGGACCTG GAGCTGGCAA TCCACCATCA CCAGTCCTTA 
TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ATGGCAAAGC ACTACTGGAT 
GTCCTGCAGC GTCCCCTGAG TCCTGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC 
TCCAAGGCGG TCCACCAGGT GCTGGATGTG GTCCACGAGG TGCTGCACCA TCAGCGACGG 
CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTTCACC AGCGGCTGCA GCTGTGTGTC 
TTCCAGCAGG ATGTGCAGCA GGTGCTGGAC TGGATTGAAA ACCATGGCGA GGCCTTTCTC 
AGCAAACACA CTGGGGTTGG CAAGTCCTTG CATCGAGCAC GTGCACTACA GAAGAGGCAT 
GATGACTTTG AAGAAGTGGC ACAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCA 
GCGGAGCAGC TGGCTCAGAC TGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCCCGG 
CACCTGGAGG TGCGCATCCA AGACTTTGTG CGCAGAGTGG AGCAGCGGAA GCTGCTTCTG 
GACATGTCGG TCTCTTTCCA CACGCACACC AAAGAGCTGT GGACATGGAT GGAGGACCTT 
CAGAAGGAGG TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAGCTGATC 
AAGCAGTTCC AGCAGCAGCA GACCGCCACC CTGGATGCCA CTCTCAATGT CATCAAGGAA 
GGCGAAGACC TTATCCAGCA GCTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCACCGAG 
GCCAGGGACT CAGCGATGTC CAACAACAAG ACACCCCACA GTAGCTCTAT CAGCCATATT 
GAGTCTGTCC TCCAGCAGCT GGACGATGCT CAGGTACAGA TGGAAGAGCT GTTTCACGAG 
CGGAAGATCA AGCTGGACAT CTTCCTACAG CTGCGCATCT TTGAACAGTA CACCATTGAG 
GTCACTGCAG AGCTGGATGC CTGGAACGAG GACTTGCTCC GGCAGATGAA TGATTTCAAC 
ACAGAGGATC TGACCCTGGC TGAGCAGCGG CTACAGCGCC ACACAGAACG GAAGCTTGCC 
ATGAACAACA TGACGTTCGA GGTCATCCAG CAGGGCCAGG ACCTGCACCA GTACATCATG 
GAAGTGCAAG CGTCAGGAAT CGAGTTAATC TGTGAGAAGG ACCTTGACCT GGCAGCTCAG 
GTGCAGGAGC TGCTGGAGTT TCTCCATGAG AAGCAGCACG AGCTGGAGCT CAATGCGGAG 
CAGACGCACA AGCGGCTAGA GCAGTGTCTC CAGCTGAGGC ACCTCCAGGC TGAGGTCAAA 
CAGGTTCTGG GATGGATCCG CAATGGAGAG TCGATGCTCA ACGCCAGCCT GGTCAACGCC 
AGCTCTTTGT CGGAGGCAGA GCAGCTGCAG CGGGAGCATG AACAGTTCCA GCTAGCCATC 
GAGTCCCTCT TTCATGCCAC TTCCTTGCAG AAGACGCACC AGAGCGCCCT GCAGGTACAG 
CAGAAGGCAG AGGCACTGCT CCAGGCCGGC CACTACGACG CAGACGCCAT CCGGGAATGT 
GCTGAGAAGG TGGCCCTCCA CTGGCAACAG CTCATGCTAA AGATGGAGGA CCGGCTGAAG 
CTTGTCAACG CCTCTGTGGC CTTTTACAAG ACCTCTGAGC AGGTGTGCAG TGTTCTGGAG 
AGCTTGGAGC AGGAGTACCG GAGAGACGAG GACTGGTGTG GTGGGAGAGA CAAGCTGGGG 
CCGGCGGCAG AGATGGACCA CGTTATTCCA CTCCTCAGCA AGCACCTGGA GCAGAAGGAG 
GCCTTCCTGA AGGCCTGCAC GCTGGCAAGG CGGAACGCCG AGGTGTTCCT CAAATACATC 
CACAGGAACA ATGTGAGCAT GCCTAGTGTT GCCAGCCACA CCCGGGGGCC TGAACAGCAA 
GTAAAAGCCA TCTTGAGCGA GCTCTTGCAG AGGGAGAATC GAGTCCTGCA CTTCTGGACC 
TTGAAGAAGC GGCGGTTAGA CCAGTGTCAG CAATATGTGG TGTTTGAGCG CAGTGCTAAG 
CAGGCACTGG ACTGGATCCA AGAAACAGGA GAATATTACC TCTCAACACA TACCTCCACC 
GGAGAGACCA CGGAGGAGAC TCAGGAGCTG CTGAAAGAGT ATGGAGAATT CAGGGTGCCT 
GCCAAGCAAA CGAAGGAGAA GGTGAAACTT CTGATCCAGC TAGCAGACAG CTTTGTGGAA 
AAGGGCCACA TCCACGCCAC AGAGATACGG AAATGGGTGA CCACGGTGGA CAAGCACTAC 
CGCGATTTCT CCCTGAGGAT GGGGAAGTAC CGGTACTCGC TGGAGAAGGC GCTGGGGGTC 
AACACGGAGG ATAACAAGGA CCTAGAGCTG GACATCATCC CAGCAAGCCT TTCGGATCGG 
GAGGTCAAGC TCCGGGACGC TAACCATGAG ATCAATGAAG AGAAACGGAA GTCAGCTCGA 
AAGAAAGAGT TCATTATGGC TGAACTGCTC CAAACTGAGA AGGCTTATGT AAGGGACTTG 
CACGAGTGCT TGGAGACCTA CCTGTGGGAG ATGACTAGTG GTGTGGAAGA GATACCCCCC 
GGCATCCTCA ATAAGGAACA TATCATCTTC GGCAACATCC AGGAGATCTA TGACTTCCAT 
AACAACATCT TCCTCAAAGA GCTGGAGAAG TACGAGCAGC TGCCTGAGGA TGTGGGACAC 
TGCTTTGTTA CCTGGGCAGA CAAATTTCAG ATGTATGTCA CCTACTGTAA AAACAAGCCT 
GATTCCAACC AGCTGATCCT GGAGCATGCG GGCACCTTCT TTGATGAGAT CCAGCAGCGG 
CATGGCCTGG CCAACTCCAT CTCCTCCTAC CTAATTAAGC CTGTCCAGAG GGTTACCAAG 
TACCAGCTGC TCCTGAAGGA ACTTCTAACT TGCTGTGAAG AAGGGAAAGG AGAGCTGAAG 
GATGGCCTGG AGGTGATGCT CAGTGTCCCC AAGAAAGCCA ACGATGCCAT GCATGTCAGC 
ATGCTGGAAG GGTTCGATGA AAACTTGGAT GTACAGGGGG AGTTGATTCT CCAGGATGCC 
TTTCAAGTGT GGGACCCAAA GTCGCTGATC CGGAAGGGGC GGGAGCGCCA TCTGTTCCTC 
TTTGAGATCT CCTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACGAAATAT 
GTTTACAAGA ACAAGCTACT GACCTCAGAG CTGGGTGTGA CCGAGCATGT AGAAGGTGAC 
CCCTGCAAAT TCGCCTTGTG GTCTGGGCGC ACCCCATCCT CAGACAATAA AACAGTGCTC 
AAAGCTTCCA ACATCGAAAC CAAGCAGGAA TGGATCAAGA ACATTCGAGA AGTGATTCAG 
GAGAGGATCA TTCATCTTAA AGGCGCTTTA AAGGAGCCAA TTCAGCTCCC CAAAACACCA 
GCCAAACTGA GGAACAATAG TAAGAGGGAT GGGGTAGAGG ATGGTGACAG CCAAGGGGAT 
GGGAGCAGCC AGCCAGATAC CATATCCATT GCCTCCAGGA CCTCTCAGAA CACAGTGGAA 
AGTGACAAGC TCTCAGGTGG ATGTGAGCTG ACCGTGGTCC TCCAGGACTT CAGCGCAGGC 
CACAGCAGCG AGCTGAGCAT CCAGGTGGGC CAGACGGTGG AGCTGCTGGA ACGACCGAGT 
GAGAGGCCTG GCTGGTGTCT GGTCCGCACC ACAGAGCGGA GCCCCCCACA GGAAGGCCTG 
GTACCCAGCA GCGCCCTGTG CATCTCACAC TCTCGCAGCA GCGTGGAAAT GGACTGCTTC 
TTCCCCTTGA AAGATTCATA CTCTCATTCC TCCAGTGAGA ATGGAGGCAA ATCCGAGTCC 
GTGGCCCACC TGCAGTCTCA GCCCTCCCTG AACTCCATCC ACAGTTCCCC TGGTCCCAAG 
CGCTCCACCA ACACCCTCAA AAAGTGGCTG ACCAGCCCCG TGCGCCGCCT CAACAGCGGA 
AAAGCCGACG GGAACATCAA AAAGCAGAAG AAAGTCCGGG ATGGGCGGAA GAGCTTCGAT 
CTGGGATCGC CCAAGCCTGG GGATGAGACG ACACCTCAGG GAGACAGCGC TGACGAGAAG 
AGCAAGAAGG GTTGGGGCGA AGACGAGCCA GATGAAGAGT CACACACACC CCTCCCTCCG 
CCCATGAAGA TCTTTGACAA CGACCCCACA CAGGATGAGA TGTCGTCTTT GCTAGCAGCC 
CGGCAGGCTC CCCCTGACGT GCCTACTGCT GCAGACCTTG TCAGTGCAAT AGAAAAGTTG 
GTCAAAAATA AGCTGACTCT GGAAGGAGGC TCTTACCGGG GGAGCTTGAA GGACCCTACA 
GGCTGCCTGA ATGAGGGGAT GACCCCTCCC ACACCTCCTA GAAACCTGGA AGAAGAACAG 
AAAGCCAAGG CGCTGAGAGG CAGGATGTTT GTCCTGAATG AGCTGGTACA GACAGAGAAG 
GACTACGTCA AGGACCTGGG GATTGTGGTG GAGGGCTTCA TGAAGAGAAT AGAAGAGAAG 
GGGGTCCCTG AGGACATGCG AGGGAAGGAA AAGATCGTGT TTGGAAATAT CCACCAGATT 
TACGACTGGC ATAAAGATTT TTTCCTGGCT GAACTGGAAA AATGTATCCA AGAGCAAGAC 
AGATTGGCCC AGCTCTTCAT TAAACACGAG CGGAAGCTGC ACATCTACGT GTGGTACTGC 
CAGAACAAGC CGCGTTCCGA GTACATCGTG GCTGAGTACG ATGCCTATTT TGAGGAGGTT 
AAACAAGAGA TCAATCAAAG GCTGACCCTT AGTGACTTCC TGATCAAGCC CATTCAGAGA 
ATAACAAAGT ACCAGCTGCT ACTCAAGGAC TTCCTGCGAT ACAGCGAGAA GGCTGGTTTG 
GAGTGTTCCG ATATTGAGAA AGCAGTGGAG TTAATGTGCC TTGTCCCCAA GCGCTGTAAT 
GACATGATGA ATCTGGGACG CCTGCAGGGC TTCGAGGGCA CCCTGACCGC CCAGGGCAAG 
CTACTGCAGC AGGACACATT CTATGTGATC GAGCTGGATG CCGGCATGCA GTCCCGGACC 
AAGGAGAGGC GTGTGTTTCT CTTTGAGCAG ATTGTCATCT TCAGTGAACT GCTCCGAAAG 
GGGTCCCTCA CCCCAGGCTA CATGTTCAAA AGAAGCATCA AGATGAATTA CTTGGTCCTG 
GAAGACAATG TGGATGGTGA CCCCTGCAAA TTTGCACTCA TGAACAGGGA GACTTCAGAA 
AGGGTCATTC TACAGGCTGC CAACTCAGAC ATCCAGCAGG CCTGGGTGCA GGACATCAAC 
CAAGTCCTAG AAACACAGAG GGACTTCTTA AATGCACTAC AGTCACCCAT TGAGTATCAG 
CGGAAGGAGA GAAGCACAGC TGTGATCCGG TCCCAGCCCC CGAGGGTTCC ACAAGCCAGC 
CCCAGGCCCT ACTCTTCTGG CCCGGTGGGC TCAGAGAAGC CCCCCAAGGG CTCCAGCTAT 
AACCCGCCTC TGCCACCCCT GAAGATATCT ACCTCTAATG GCAGTCCAGG GTTCGACTAC 
CATCAGCCTG GGGACAAGTT TGACGCTAGC AAGCAGAACG ACCTGGGAGG CTGCAATGGG 
ACATCAACCA TGACTGTAAT CAAAGATTAC TATGCACTGA AGGAGAATGA AATCTGCGTG 
AGCCAAGGTG AGGTGGTACA GGTCCTCGCC GTCAACCAGC AGAACATGTG TCTGGTGTAC 
CAGCCTGCCA GCGACCATTC CCCGGCAGCA GAGGGCTGGG TCCCAGGCAG CATCCTGGCG 
CCCCTCGCCA AAGCCACAGC AGCGGCCGAA AGTAGTGACG GGAGCATCAA GAAGTCATGC 
TCTTGGCACA CTCTACGCAT GAGAAAGCGG GCAGACGTGG AGAACTCGGG TAAAAATGAA 
GCCACAGGGC CTCGTAAACC CAAGGATATT CTGGGCAACA AAGTCTCTGT TAAAGAGACG 
AACAGTTCCG AGGAGTCAGA GTGTGATGAT CTTGACCCTA ATACTAGTAT GGAGATCTTG 
AATCCAAATT TCATCCAGGA AGTGGCTCCA GAATTCCTCG TGCCCTTAGT AGATGTGACC 
TGTTTGCTTG GAGACACAGT GCTACTGCAG TGTAAAGCCT GTGGGCGGCC AAAGCCCAGC 
ATCACCTGGA AGGGTCCGGA TCAGAACATC CTAGACACTG ACAACAGTTC AGCAACATAC 
ACCATCTCCT CCTGTGATTC TGGGGAAAGC ACCCTCAAGA TCTGCAACCT GATGCCCCAG 
GATAGTGGGA TTTATACCTG CATAGCAGCA AACGACCACG GGACTGCATC CACATCTGCT 
ACAGTTAAGG TGCAAGGTGT CCCCGCAGCC CCCAACCGCC CAATCGCACA GGAGAGGAGC 
TGCACCTCCG TCATCCTCCG CTGGCTGCCC CCAGCCAGCA CCGGAAACTG CACTATTTCG 
GGGTACACAG TGGAGTATCG CGAGGAAGGT TCCCAGGTCT GGCAGCAATC CGTAGCTTCG 
ACCTTGGACA CTTACCTGGT CATTGAAGAC CTTAGTCCTG GGTGCCCTTA TCAGTTCAGG 
GTCAGTGCCA GCAACCCCTG GGGAATCAGC CTTCCCAGTG AGCCCTCGGA GTTTGTGCGC 
CTTCCAGAGT ACGATGCAGC CGCCGACGGT GCCACCATTT CTTGGAAGGA AAATTTTGAT 
TCTGCTTACA CTGAACTGAA CGAGATTGGC AGAGGCCGTT TCTCTATTGT AAAGAAATGC 
ATTCACAAAG CTACCCGCAA AGATGTTGCT GTGAAATTTG TGAGCAAAAA AATGAAGAAG 
AAGGAGCAGG CTGCTCACGA GGCCGCCCTG CTCCAGCACC TGCAGCACCC TCAGTATGTC 
ACGCTCCATG ACACCTACGA GTCCCCCACA TCCTACATCC TGATTTTGGA ACTGATGGAT 
GACGGCCGAC TCTTGGACTA CCTTATGAAT CATGATGAGC TGATGGAAGA AAAGGTAGCT 
TTCTACATTC GAGACATCAT GGAAGCTCTG CAGTACCTGC ACAACTGTAG GGTCGCACAT 
TTGGACATAA AGCCCGAAAA CCTGCTCATT GACCTCCGGA TCCCAGTGCC TCGAGTGAAG 
CTCATCGACT TGGAAGACGC TGTCCAGATC TCAGGCCACT TCCATATCCA CCATCTGCTG 
GGGAACCCTG AGTTCGCTGC CCCCGAAGTG ATCCAAGGCA TCCCTGTCTC CCTGGGCACA 
GACATCTGGA GCATCGGGGT TCTGACATAT GTCATGCTTA GTGGGGTCTC CCCCTTCTTA 
GATGAGAGCA AAGAGGAAAC CTGTATCAAC GTGTGCAGGG TGGACTTTAG CTTCCCCCAC 
GAGTACTTCT GCGGTGTGAG CAATGCTGCC AGGGATTTCA TCAACGTAAT CTTACAGGAA 
GACTTCCGGA GGCGGCCCAC AGCAGCCACG TGCCTGCAGC ATCCCTGGCT ACAGCCCCAC 
AACGGCAGCT ACTCCAAGAT CCCCTTGGAC ACCTCCCGTC TGGCCTGCTT CATAGAACGC 
CGCAAGCACC AGAATGATGT GCGGCCCATT CCCAATGTCA AGAGTTACAT CGTCAATAGG 
GTGAACCAAG GGACCTCGCT ATCCCACAAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522363.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522426.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X6, mRNA.

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CloneID	OMu52279
Clone ID Related Accession (Same CDS sequence)	XM_006522363.3 , XM_006522363.4
Accession Version	XM_006522363.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522363.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGAT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC TATCCTAAAG 
GAGAAAGTGG CCTTTGTGTC TGGGGGGCGT GACAAGCGAG GTGGACCCAT CCTGACCTTC 
CCTGCCCGAA GCAATCATGA CAGAATAAGA CAGGAAGACC TACGGAAACT TGTGACGTAT 
TTGGCCAGCG TGCCAAGTGA GGACGTTTGC AAACGTGGCT TCACCGTCAT CATCGACATG 
CGGGGCTCCA AGTGGGATCT CATCAAGCCC CTCCTCAAGA CGCTGCAGGA AGCCTTCCCC 
GCCGAGATCC ATGTGGCCCT CATCATCAAG CCTGACAACT TCTGGCAGAA GCAGAAGACC 
AACTTTGGCA GCTCCAAATT CATCTTTGAG ACAAGCATGG TGTCTGTGGA GGGCCTCACG 
AAGCTGGTGG ACCCCTCCCA GCTGACAGAG GAGTTTGACG GCTCCCTGGA TTACAACCAT 
GAGGAGTGGA TTGAGTTGCG CCTCTCCCTG GAGGAGTTCT TCAACAGTGC GGTCCACCTG 
CTCTCACGCC TTGAGGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC TGTGGATGTG 
GAGGGCTCTC GGAGGCTTAT CGACGAGCAC ACGCAACTTA AGAAAAAGGT GCTGAAGGCC 
CCCGTGGAAG AGCTGGACCG GGAGGGGCAG CGTCTACTGC AGTGCATCCG CTGCAGCGAT 
GGCTTCTCAG GGCGCAACTG CATCCCAGGA AGTGCCGACT TCCAGAGCCT TGTACCCAAG 
ATCACCAGCC TCCTGGACAA GCTGCATTCC ACCCGACAAC ACCTGCACCA AATGTGGCAC 
GTGCGAAAGC TCAAGCTGGA TCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAG 
AAGATGTTCG ACTGGATAAG CCACAACAAG GAGTTATTTC TTCAGAGCCA CACAGAGATC 
GGAGTCAGCT ACCAGCATGC ACTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC 
TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TCGCTTCCCG CCTCTCAGAG 
GCCGGCCATT ATGCCTCCCA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG 
AAGAGCTTTG CCGCTGCCCT GGATGAACGC AGTACAATCC TGGCCATGTC TGCTGTGTTC 
CACCAGAAGG CAGAGCAGTT CCTGTCAGGC GTGGATGCCT GGTGTAAGAT GTGCAGCGAA 
GGCGGCCTTC CTTCTGAGAT GCAGGACCTG GAGCTGGCAA TCCACCATCA CCAGTCCTTA 
TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ATGGCAAAGC ACTACTGGAT 
GTCCTGCAGC GTCCCCTGAG TCCTGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC 
TCCAAGGCGG TCCACCAGGT GCTGGATGTG GTCCACGAGG TGCTGCACCA TCAGCGACGG 
CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTTCACC AGCGGCTGCA GCTGTGTGTC 
TTCCAGCAGG ATGTGCAGCA GGTGCTGGAC TGGATTGAAA ACCATGGCGA GGCCTTTCTC 
AGCAAACACA CTGGGGTTGG CAAGTCCTTG CATCGAGCAC GTGCACTACA GAAGAGGCAT 
GATGACTTTG AAGAAGTGGC ACAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCA 
GCGGAGCAGC TGGCTCAGAC TGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCCCGG 
CACCTGGAGG TGCGCATCCA AGACTTTGTG CGCAGAGTGG AGCAGCGGAA GCTGCTTCTG 
GACATGTCGG TCTCTTTCCA CACGCACACC AAAGAGCTGT GGACATGGAT GGAGGACCTT 
CAGAAGGAGG TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAGCTGATC 
AAGCAGTTCC AGCAGCAGCA GACCGCCACC CTGGATGCCA CTCTCAATGT CATCAAGGAA 
GGCGAAGACC TTATCCAGCA GCTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCACCGAG 
GCCAGGGACT CAGCGATGTC CAACAACAAG ACACCCCACA GTAGCTCTAT CAGCCATATT 
GAGTCTGTCC TCCAGCAGCT GGACGATGCT CAGGTACAGA TGGAAGAGCT GTTTCACGAG 
CGGAAGATCA AGCTGGACAT CTTCCTACAG CTGCGCATCT TTGAACAGTA CACCATTGAG 
GTCACTGCAG AGCTGGATGC CTGGAACGAG GACTTGCTCC GGCAGATGAA TGATTTCAAC 
ACAGAGGATC TGACCCTGGC TGAGCAGCGG CTACAGCGCC ACACAGAACG GAAGCTTGCC 
ATGAACAACA TGACGTTCGA GGTCATCCAG CAGGGCCAGG ACCTGCACCA GTACATCATG 
GAAGTGCAAG CGTCAGGAAT CGAGTTAATC TGTGAGAAGG ACCTTGACCT GGCAGCTCAG 
GTGCAGGAGC TGCTGGAGTT TCTCCATGAG AAGCAGCACG AGCTGGAGCT CAATGCGGAG 
CAGACGCACA AGCGGCTAGA GCAGTGTCTC CAGCTGAGGC ACCTCCAGGC TGAGGTCAAA 
CAGGTTCTGG GATGGATCCG CAATGGAGAG TCGATGCTCA ACGCCAGCCT GGTCAACGCC 
AGCTCTTTGT CGGAGGCAGA GCAGCTGCAG CGGGAGCATG AACAGTTCCA GCTAGCCATC 
GAGTCCCTCT TTCATGCCAC TTCCTTGCAG AAGACGCACC AGAGCGCCCT GCAGGTACAG 
CAGAAGGCAG AGGCACTGCT CCAGGCCGGC CACTACGACG CAGACGCCAT CCGGGAATGT 
GCTGAGAAGG TGGCCCTCCA CTGGCAACAG CTCATGCTAA AGATGGAGGA CCGGCTGAAG 
CTTGTCAACG CCTCTGTGGC CTTTTACAAG ACCTCTGAGC AGGTGTGCAG TGTTCTGGAG 
AGCTTGGAGC AGGAGTACCG GAGAGACGAG GACTGGTGTG GTGGGAGAGA CAAGCTGGGG 
CCGGCGGCAG AGATGGACCA CGTTATTCCA CTCCTCAGCA AGCACCTGGA GCAGAAGGAG 
GCCTTCCTGA AGGCCTGCAC GCTGGCAAGG CGGAACGCCG AGGTGTTCCT CAAATACATC 
CACAGGAACA ATGTGAGCAT GCCTAGTGTT GCCAGCCACA CCCGGGGGCC TGAACAGCAA 
GTAAAAGCCA TCTTGAGCGA GCTCTTGCAG AGGGAGAATC GAGTCCTGCA CTTCTGGACC 
TTGAAGAAGC GGCGGTTAGA CCAGTGTCAG CAATATGTGG TGTTTGAGCG CAGTGCTAAG 
CAGGCACTGG ACTGGATCCA AGAAACAGGA GAATATTACC TCTCAACACA TACCTCCACC 
GGAGAGACCA CGGAGGAGAC TCAGGAGCTG CTGAAAGAGT ATGGAGAATT CAGGGTGCCT 
GCCAAGCAAA CGAAGGAGAA GGTGAAACTT CTGATCCAGC TAGCAGACAG CTTTGTGGAA 
AAGGGCCACA TCCACGCCAC AGAGATACGG AAATGGGTGA CCACGGTGGA CAAGCACTAC 
CGCGATTTCT CCCTGAGGAT GGGGAAGTAC CGGTACTCGC TGGAGAAGGC GCTGGGGGTC 
AACACGGAGG ATAACAAGGA CCTAGAGCTG GACATCATCC CAGCAAGCCT TTCGGATCGG 
GAGGTCAAGC TCCGGGACGC TAACCATGAG ATCAATGAAG AGAAACGGAA GTCAGCTCGA 
AAGAAAGAGT TCATTATGGC TGAACTGCTC CAAACTGAGA AGGCTTATGT AAGGGACTTG 
CACGAGTGCT TGGAGACCTA CCTGTGGGAG ATGACTAGTG GTGTGGAAGA GATACCCCCC 
GGCATCCTCA ATAAGGAACA TATCATCTTC GGCAACATCC AGGAGATCTA TGACTTCCAT 
AACAACATCT TCCTCAAAGA GCTGGAGAAG TACGAGCAGC TGCCTGAGGA TGTGGGACAC 
TGCTTTGTTA CCTGGGCAGA CAAATTTCAG ATGTATGTCA CCTACTGTAA AAACAAGCCT 
GATTCCAACC AGCTGATCCT GGAGCATGCG GGCACCTTCT TTGATGAGAT CCAGCAGCGG 
CATGGCCTGG CCAACTCCAT CTCCTCCTAC CTAATTAAGC CTGTCCAGAG GGTTACCAAG 
TACCAGCTGC TCCTGAAGGA ACTTCTAACT TGCTGTGAAG AAGGGAAAGG AGAGCTGAAG 
GATGGCCTGG AGGTGATGCT CAGTGTCCCC AAGAAAGCCA ACGATGCCAT GCATGTCAGC 
ATGCTGGAAG GGTTCGATGA AAACTTGGAT GTACAGGGGG AGTTGATTCT CCAGGATGCC 
TTTCAAGTGT GGGACCCAAA GTCGCTGATC CGGAAGGGGC GGGAGCGCCA TCTGTTCCTC 
TTTGAGATCT CCTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACGAAATAT 
GTTTACAAGA ACAAGCTACT GACCTCAGAG CTGGGTGTGA CCGAGCATGT AGAAGGTGAC 
CCCTGCAAAT TCGCCTTGTG GTCTGGGCGC ACCCCATCCT CAGACAATAA AACAGTGCTC 
AAAGCTTCCA ACATCGAAAC CAAGCAGGAA TGGATCAAGA ACATTCGAGA AGTGATTCAG 
GAGAGGATCA TTCATCTTAA AGGCGCTTTA AAGGAGCCAA TTCAGCTCCC CAAAACACCA 
GCCAAACTGA GGAACAATAG TAAGAGGGAT GGGGTAGAGG ATGGTGACAG CCAAGGGGAT 
GGGAGCAGCC AGCCAGATAC CATATCCATT GCCTCCAGGA CCTCTCAGAA CACAGTGGAA 
AGTGACAAGC TCTCAGGTGG ATGTGAGCTG ACCGTGGTCC TCCAGGACTT CAGCGCAGGC 
CACAGCAGCG AGCTGAGCAT CCAGGTGGGC CAGACGGTGG AGCTGCTGGA ACGACCGAGT 
GAGAGGCCTG GCTGGTGTCT GGTCCGCACC ACAGAGCGGA GCCCCCCACA GGAAGGCCTG 
GTACCCAGCA GCGCCCTGTG CATCTCACAC TCTCGCAGCA GCGTGGAAAT GGACTGCTTC 
TTCCCCTTGA AAGATTCATA CTCTCATTCC TCCAGTGAGA ATGGAGGCAA ATCCGAGTCC 
GTGGCCCACC TGCAGTCTCA GCCCTCCCTG AACTCCATCC ACAGTTCCCC TGGTCCCAAG 
CGCTCCACCA ACACCCTCAA AAAGTGGCTG ACCAGCCCCG TGCGCCGCCT CAACAGCGGA 
AAAGCCGACG GGAACATCAA AAAGCAGAAG AAAGTCCGGG ATGGGCGGAA GAGCTTCGAT 
CTGGGATCGC CCAAGCCTGG GGATGAGACG ACACCTCAGG GAGACAGCGC TGACGAGAAG 
AGCAAGAAGG GTTGGGGCGA AGACGAGCCA GATGAAGAGT CACACACACC CCTCCCTCCG 
CCCATGAAGA TCTTTGACAA CGACCCCACA CAGGATGAGA TGTCGTCTTT GCTAGCAGCC 
CGGCAGGCTC CCCCTGACGT GCCTACTGCT GCAGACCTTG TCAGTGCAAT AGAAAAGTTG 
GTCAAAAATA AGCTGACTCT GGAAGGAGGC TCTTACCGGG GGAGCTTGAA GGACCCTACA 
GGCTGCCTGA ATGAGGGGAT GACCCCTCCC ACACCTCCTA GAAACCTGGA AGAAGAACAG 
AAAGCCAAGG CGCTGAGAGG CAGGATGTTT GTCCTGAATG AGCTGGTACA GACAGAGAAG 
GACTACGTCA AGGACCTGGG GATTGTGGTG GAGGGCTTCA TGAAGAGAAT AGAAGAGAAG 
GGGGTCCCTG AGGACATGCG AGGGAAGGAA AAGATCGTGT TTGGAAATAT CCACCAGATT 
TACGACTGGC ATAAAGATTT TTTCCTGGCT GAACTGGAAA AATGTATCCA AGAGCAAGAC 
AGATTGGCCC AGCTCTTCAT TAAACACGAG CGGAAGCTGC ACATCTACGT GTGGTACTGC 
CAGAACAAGC CGCGTTCCGA GTACATCGTG GCTGAGTACG ATGCCTATTT TGAGGAGGTT 
AAACAAGAGA TCAATCAAAG GCTGACCCTT AGTGACTTCC TGATCAAGCC CATTCAGAGA 
ATAACAAAGT ACCAGCTGCT ACTCAAGGAC TTCCTGCGAT ACAGCGAGAA GGCTGGTTTG 
GAGTGTTCCG ATATTGAGAA AGCAGTGGAG TTAATGTGCC TTGTCCCCAA GCGCTGTAAT 
GACATGATGA ATCTGGGACG CCTGCAGGGC TTCGAGGGCA CCCTGACCGC CCAGGGCAAG 
CTACTGCAGC AGGACACATT CTATGTGATC GAGCTGGATG CCGGCATGCA GTCCCGGACC 
AAGGAGAGGC GTGTGTTTCT CTTTGAGCAG ATTGTCATCT TCAGTGAACT GCTCCGAAAG 
GGGTCCCTCA CCCCAGGCTA CATGTTCAAA AGAAGCATCA AGATGAATTA CTTGGTCCTG 
GAAGACAATG TGGATGGTGA CCCCTGCAAA TTTGCACTCA TGAACAGGGA GACTTCAGAA 
AGGGTCATTC TACAGGCTGC CAACTCAGAC ATCCAGCAGG CCTGGGTGCA GGACATCAAC 
CAAGTCCTAG AAACACAGAG GGACTTCTTA AATGCACTAC AGTCACCCAT TGAGTATCAG 
CGGAAGGAGA GAAGCACAGC TGTGATCCGG TCCCAGCCCC CGAGGGTTCC ACAAGCCAGC 
CCCAGGCCCT ACTCTTCTGG CCCGGTGGGC TCAGAGAAGC CCCCCAAGGG CTCCAGCTAT 
AACCCGCCTC TGCCACCCCT GAAGATATCT ACCTCTAATG GCAGTCCAGG GTTCGACTAC 
CATCAGCCTG GGGACAAGTT TGACGCTAGC AAGCAGAACG ACCTGGGAGG CTGCAATGGG 
ACATCAACCA TGACTGTAAT CAAAGATTAC TATGCACTGA AGGAGAATGA AATCTGCGTG 
AGCCAAGGTG AGGTGGTACA GGTCCTCGCC GTCAACCAGC AGAACATGTG TCTGGTGTAC 
CAGCCTGCCA GCGACCATTC CCCGGCAGCA GAGGGCTGGG TCCCAGGCAG CATCCTGGCG 
CCCCTCGCCA AAGCCACAGC AGCGGCCGAA AGTAGTGACG GGAGCATCAA GAAGTCATGC 
TCTTGGCACA CTCTACGCAT GAGAAAGCGG GCAGACGTGG AGAACTCGGG TAAAAATGAA 
GCCACAGGGC CTCGTAAACC CAAGGATATT CTGGGCAACA AAGTCTCTGT TAAAGAGACG 
AACAGTTCCG AGGAGTCAGA GTGTGATGAT CTTGACCCTA ATACTAGTAT GGAGATCTTG 
AATCCAAATT TCATCCAGGA AGTGGCTCCA GAATTCCTCG TGCCCTTAGT AGATGTGACC 
TGTTTGCTTG GAGACACAGT GCTACTGCAG TGTAAAGCCT GTGGGCGGCC AAAGCCCAGC 
ATCACCTGGA AGGGTCCGGA TCAGAACATC CTAGACACTG ACAACAGTTC AGCAACATAC 
ACCATCTCCT CCTGTGATTC TGGGGAAAGC ACCCTCAAGA TCTGCAACCT GATGCCCCAG 
GATAGTGGGA TTTATACCTG CATAGCAGCA AACGACCACG GGACTGCATC CACATCTGCT 
ACAGTTAAGG TGCAAGGTGT CCCCGCAGCC CCCAACCGCC CAATCGCACA GGAGAGGAGC 
TGCACCTCCG TCATCCTCCG CTGGCTGCCC CCAGCCAGCA CCGGAAACTG CACTATTTCG 
GGGTACACAG TGGAGTATCG CGAGGAAGGT TCCCAGGTCT GGCAGCAATC CGTAGCTTCG 
ACCTTGGACA CTTACCTGGT CATTGAAGAC CTTAGTCCTG GGTGCCCTTA TCAGTTCAGG 
GTCAGTGCCA GCAACCCCTG GGGAATCAGC CTTCCCAGTG AGCCCTCGGA GTTTGTGCGC 
CTTCCAGAGT ACGATGCAGC CGCCGACGGT GCCACCATTT CTTGGAAGGA AAATTTTGAT 
TCTGCTTACA CTGAACTGAA CGAGATTGGC AGAGGCCGTT TCTCTATTGT AAAGAAATGC 
ATTCACAAAG CTACCCGCAA AGATGTTGCT GTGAAATTTG TGAGCAAAAA AATGAAGAAG 
AAGGAGCAGG CTGCTCACGA GGCCGCCCTG CTCCAGCACC TGCAGCACCC TCAGTATGTC 
ACGCTCCATG ACACCTACGA GTCCCCCACA TCCTACATCC TGATTTTGGA ACTGATGGAT 
GACGGCCGAC TCTTGGACTA CCTTATGAAT CATGATGAGC TGATGGAAGA AAAGGTAGCT 
TTCTACATTC GAGACATCAT GGAAGCTCTG CAGTACCTGC ACAACTGTAG GGTCGCACAT 
TTGGACATAA AGCCCGAAAA CCTGCTCATT GACCTCCGGA TCCCAGTGCC TCGAGTGAAG 
CTCATCGACT TGGAAGACGC TGTCCAGATC TCAGGCCACT TCCATATCCA CCATCTGCTG 
GGGAACCCTG AGTTCGCTGC CCCCGAAGTG ATCCAAGGCA TCCCTGTCTC CCTGGGCACA 
GACATCTGGA GCATCGGGGT TCTGACATAT GTCATGCTTA GTGGGGTCTC CCCCTTCTTA 
GATGAGAGCA AAGAGGAAAC CTGTATCAAC GTGTGCAGGG TGGACTTTAG CTTCCCCCAC 
GAGTACTTCT GCGGTGTGAG CAATGCTGCC AGGGATTTCA TCAACGTAAT CTTACAGGAA 
GACTTCCGGA GGCGGCCCAC AGCAGCCACG TGCCTGCAGC ATCCCTGGCT ACAGCCCCAC 
AACGGCAGCT ACTCCAAGAT CCCCTTGGAC ACCTCCCGTC TGGCCTGCTT CATAGAACGC 
CGCAAGCACC AGAATGATGT GCGGCCCATT CCCAATGTCA AGAGTTACAT CGTCAATAGG 
GTGAACCAAG GGACCTCGCT ATCCCACAAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522426.1
CDS	3337..12312
Translation	MTDRFWDQWYLWYLRLLRLLDRGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_006522363.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522363.4

ORF Insert Sequence:

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGAT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC TATCCTAAAG 
GAGAAAGTGG CCTTTGTGTC TGGGGGGCGT GACAAGCGAG GTGGACCCAT CCTGACCTTC 
CCTGCCCGAA GCAATCATGA CAGAATAAGA CAGGAAGACC TACGGAAACT TGTGACGTAT 
TTGGCCAGCG TGCCAAGTGA GGACGTTTGC AAACGTGGCT TCACCGTCAT CATCGACATG 
CGGGGCTCCA AGTGGGATCT CATCAAGCCC CTCCTCAAGA CGCTGCAGGA AGCCTTCCCC 
GCCGAGATCC ATGTGGCCCT CATCATCAAG CCTGACAACT TCTGGCAGAA GCAGAAGACC 
AACTTTGGCA GCTCCAAATT CATCTTTGAG ACAAGCATGG TGTCTGTGGA GGGCCTCACG 
AAGCTGGTGG ACCCCTCCCA GCTGACAGAG GAGTTTGACG GCTCCCTGGA TTACAACCAT 
GAGGAGTGGA TTGAGTTGCG CCTCTCCCTG GAGGAGTTCT TCAACAGTGC GGTCCACCTG 
CTCTCACGCC TTGAGGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC TGTGGATGTG 
GAGGGCTCTC GGAGGCTTAT CGACGAGCAC ACGCAACTTA AGAAAAAGGT GCTGAAGGCC 
CCCGTGGAAG AGCTGGACCG GGAGGGGCAG CGTCTACTGC AGTGCATCCG CTGCAGCGAT 
GGCTTCTCAG GGCGCAACTG CATCCCAGGA AGTGCCGACT TCCAGAGCCT TGTACCCAAG 
ATCACCAGCC TCCTGGACAA GCTGCATTCC ACCCGACAAC ACCTGCACCA AATGTGGCAC 
GTGCGAAAGC TCAAGCTGGA TCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAG 
AAGATGTTCG ACTGGATAAG CCACAACAAG GAGTTATTTC TTCAGAGCCA CACAGAGATC 
GGAGTCAGCT ACCAGCATGC ACTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC 
TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TCGCTTCCCG CCTCTCAGAG 
GCCGGCCATT ATGCCTCCCA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG 
AAGAGCTTTG CCGCTGCCCT GGATGAACGC AGTACAATCC TGGCCATGTC TGCTGTGTTC 
CACCAGAAGG CAGAGCAGTT CCTGTCAGGC GTGGATGCCT GGTGTAAGAT GTGCAGCGAA 
GGCGGCCTTC CTTCTGAGAT GCAGGACCTG GAGCTGGCAA TCCACCATCA CCAGTCCTTA 
TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ATGGCAAAGC ACTACTGGAT 
GTCCTGCAGC GTCCCCTGAG TCCTGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC 
TCCAAGGCGG TCCACCAGGT GCTGGATGTG GTCCACGAGG TGCTGCACCA TCAGCGACGG 
CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTTCACC AGCGGCTGCA GCTGTGTGTC 
TTCCAGCAGG ATGTGCAGCA GGTGCTGGAC TGGATTGAAA ACCATGGCGA GGCCTTTCTC 
AGCAAACACA CTGGGGTTGG CAAGTCCTTG CATCGAGCAC GTGCACTACA GAAGAGGCAT 
GATGACTTTG AAGAAGTGGC ACAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCA 
GCGGAGCAGC TGGCTCAGAC TGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCCCGG 
CACCTGGAGG TGCGCATCCA AGACTTTGTG CGCAGAGTGG AGCAGCGGAA GCTGCTTCTG 
GACATGTCGG TCTCTTTCCA CACGCACACC AAAGAGCTGT GGACATGGAT GGAGGACCTT 
CAGAAGGAGG TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAGCTGATC 
AAGCAGTTCC AGCAGCAGCA GACCGCCACC CTGGATGCCA CTCTCAATGT CATCAAGGAA 
GGCGAAGACC TTATCCAGCA GCTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCACCGAG 
GCCAGGGACT CAGCGATGTC CAACAACAAG ACACCCCACA GTAGCTCTAT CAGCCATATT 
GAGTCTGTCC TCCAGCAGCT GGACGATGCT CAGGTACAGA TGGAAGAGCT GTTTCACGAG 
CGGAAGATCA AGCTGGACAT CTTCCTACAG CTGCGCATCT TTGAACAGTA CACCATTGAG 
GTCACTGCAG AGCTGGATGC CTGGAACGAG GACTTGCTCC GGCAGATGAA TGATTTCAAC 
ACAGAGGATC TGACCCTGGC TGAGCAGCGG CTACAGCGCC ACACAGAACG GAAGCTTGCC 
ATGAACAACA TGACGTTCGA GGTCATCCAG CAGGGCCAGG ACCTGCACCA GTACATCATG 
GAAGTGCAAG CGTCAGGAAT CGAGTTAATC TGTGAGAAGG ACCTTGACCT GGCAGCTCAG 
GTGCAGGAGC TGCTGGAGTT TCTCCATGAG AAGCAGCACG AGCTGGAGCT CAATGCGGAG 
CAGACGCACA AGCGGCTAGA GCAGTGTCTC CAGCTGAGGC ACCTCCAGGC TGAGGTCAAA 
CAGGTTCTGG GATGGATCCG CAATGGAGAG TCGATGCTCA ACGCCAGCCT GGTCAACGCC 
AGCTCTTTGT CGGAGGCAGA GCAGCTGCAG CGGGAGCATG AACAGTTCCA GCTAGCCATC 
GAGTCCCTCT TTCATGCCAC TTCCTTGCAG AAGACGCACC AGAGCGCCCT GCAGGTACAG 
CAGAAGGCAG AGGCACTGCT CCAGGCCGGC CACTACGACG CAGACGCCAT CCGGGAATGT 
GCTGAGAAGG TGGCCCTCCA CTGGCAACAG CTCATGCTAA AGATGGAGGA CCGGCTGAAG 
CTTGTCAACG CCTCTGTGGC CTTTTACAAG ACCTCTGAGC AGGTGTGCAG TGTTCTGGAG 
AGCTTGGAGC AGGAGTACCG GAGAGACGAG GACTGGTGTG GTGGGAGAGA CAAGCTGGGG 
CCGGCGGCAG AGATGGACCA CGTTATTCCA CTCCTCAGCA AGCACCTGGA GCAGAAGGAG 
GCCTTCCTGA AGGCCTGCAC GCTGGCAAGG CGGAACGCCG AGGTGTTCCT CAAATACATC 
CACAGGAACA ATGTGAGCAT GCCTAGTGTT GCCAGCCACA CCCGGGGGCC TGAACAGCAA 
GTAAAAGCCA TCTTGAGCGA GCTCTTGCAG AGGGAGAATC GAGTCCTGCA CTTCTGGACC 
TTGAAGAAGC GGCGGTTAGA CCAGTGTCAG CAATATGTGG TGTTTGAGCG CAGTGCTAAG 
CAGGCACTGG ACTGGATCCA AGAAACAGGA GAATATTACC TCTCAACACA TACCTCCACC 
GGAGAGACCA CGGAGGAGAC TCAGGAGCTG CTGAAAGAGT ATGGAGAATT CAGGGTGCCT 
GCCAAGCAAA CGAAGGAGAA GGTGAAACTT CTGATCCAGC TAGCAGACAG CTTTGTGGAA 
AAGGGCCACA TCCACGCCAC AGAGATACGG AAATGGGTGA CCACGGTGGA CAAGCACTAC 
CGCGATTTCT CCCTGAGGAT GGGGAAGTAC CGGTACTCGC TGGAGAAGGC GCTGGGGGTC 
AACACGGAGG ATAACAAGGA CCTAGAGCTG GACATCATCC CAGCAAGCCT TTCGGATCGG 
GAGGTCAAGC TCCGGGACGC TAACCATGAG ATCAATGAAG AGAAACGGAA GTCAGCTCGA 
AAGAAAGAGT TCATTATGGC TGAACTGCTC CAAACTGAGA AGGCTTATGT AAGGGACTTG 
CACGAGTGCT TGGAGACCTA CCTGTGGGAG ATGACTAGTG GTGTGGAAGA GATACCCCCC 
GGCATCCTCA ATAAGGAACA TATCATCTTC GGCAACATCC AGGAGATCTA TGACTTCCAT 
AACAACATCT TCCTCAAAGA GCTGGAGAAG TACGAGCAGC TGCCTGAGGA TGTGGGACAC 
TGCTTTGTTA CCTGGGCAGA CAAATTTCAG ATGTATGTCA CCTACTGTAA AAACAAGCCT 
GATTCCAACC AGCTGATCCT GGAGCATGCG GGCACCTTCT TTGATGAGAT CCAGCAGCGG 
CATGGCCTGG CCAACTCCAT CTCCTCCTAC CTAATTAAGC CTGTCCAGAG GGTTACCAAG 
TACCAGCTGC TCCTGAAGGA ACTTCTAACT TGCTGTGAAG AAGGGAAAGG AGAGCTGAAG 
GATGGCCTGG AGGTGATGCT CAGTGTCCCC AAGAAAGCCA ACGATGCCAT GCATGTCAGC 
ATGCTGGAAG GGTTCGATGA AAACTTGGAT GTACAGGGGG AGTTGATTCT CCAGGATGCC 
TTTCAAGTGT GGGACCCAAA GTCGCTGATC CGGAAGGGGC GGGAGCGCCA TCTGTTCCTC 
TTTGAGATCT CCTTGGTGTT TAGCAAAGAG ATCAAAGACT CTTCAGGACA CACGAAATAT 
GTTTACAAGA ACAAGCTACT GACCTCAGAG CTGGGTGTGA CCGAGCATGT AGAAGGTGAC 
CCCTGCAAAT TCGCCTTGTG GTCTGGGCGC ACCCCATCCT CAGACAATAA AACAGTGCTC 
AAAGCTTCCA ACATCGAAAC CAAGCAGGAA TGGATCAAGA ACATTCGAGA AGTGATTCAG 
GAGAGGATCA TTCATCTTAA AGGCGCTTTA AAGGAGCCAA TTCAGCTCCC CAAAACACCA 
GCCAAACTGA GGAACAATAG TAAGAGGGAT GGGGTAGAGG ATGGTGACAG CCAAGGGGAT 
GGGAGCAGCC AGCCAGATAC CATATCCATT GCCTCCAGGA CCTCTCAGAA CACAGTGGAA 
AGTGACAAGC TCTCAGGTGG ATGTGAGCTG ACCGTGGTCC TCCAGGACTT CAGCGCAGGC 
CACAGCAGCG AGCTGAGCAT CCAGGTGGGC CAGACGGTGG AGCTGCTGGA ACGACCGAGT 
GAGAGGCCTG GCTGGTGTCT GGTCCGCACC ACAGAGCGGA GCCCCCCACA GGAAGGCCTG 
GTACCCAGCA GCGCCCTGTG CATCTCACAC TCTCGCAGCA GCGTGGAAAT GGACTGCTTC 
TTCCCCTTGA AAGATTCATA CTCTCATTCC TCCAGTGAGA ATGGAGGCAA ATCCGAGTCC 
GTGGCCCACC TGCAGTCTCA GCCCTCCCTG AACTCCATCC ACAGTTCCCC TGGTCCCAAG 
CGCTCCACCA ACACCCTCAA AAAGTGGCTG ACCAGCCCCG TGCGCCGCCT CAACAGCGGA 
AAAGCCGACG GGAACATCAA AAAGCAGAAG AAAGTCCGGG ATGGGCGGAA GAGCTTCGAT 
CTGGGATCGC CCAAGCCTGG GGATGAGACG ACACCTCAGG GAGACAGCGC TGACGAGAAG 
AGCAAGAAGG GTTGGGGCGA AGACGAGCCA GATGAAGAGT CACACACACC CCTCCCTCCG 
CCCATGAAGA TCTTTGACAA CGACCCCACA CAGGATGAGA TGTCGTCTTT GCTAGCAGCC 
CGGCAGGCTC CCCCTGACGT GCCTACTGCT GCAGACCTTG TCAGTGCAAT AGAAAAGTTG 
GTCAAAAATA AGCTGACTCT GGAAGGAGGC TCTTACCGGG GGAGCTTGAA GGACCCTACA 
GGCTGCCTGA ATGAGGGGAT GACCCCTCCC ACACCTCCTA GAAACCTGGA AGAAGAACAG 
AAAGCCAAGG CGCTGAGAGG CAGGATGTTT GTCCTGAATG AGCTGGTACA GACAGAGAAG 
GACTACGTCA AGGACCTGGG GATTGTGGTG GAGGGCTTCA TGAAGAGAAT AGAAGAGAAG 
GGGGTCCCTG AGGACATGCG AGGGAAGGAA AAGATCGTGT TTGGAAATAT CCACCAGATT 
TACGACTGGC ATAAAGATTT TTTCCTGGCT GAACTGGAAA AATGTATCCA AGAGCAAGAC 
AGATTGGCCC AGCTCTTCAT TAAACACGAG CGGAAGCTGC ACATCTACGT GTGGTACTGC 
CAGAACAAGC CGCGTTCCGA GTACATCGTG GCTGAGTACG ATGCCTATTT TGAGGAGGTT 
AAACAAGAGA TCAATCAAAG GCTGACCCTT AGTGACTTCC TGATCAAGCC CATTCAGAGA 
ATAACAAAGT ACCAGCTGCT ACTCAAGGAC TTCCTGCGAT ACAGCGAGAA GGCTGGTTTG 
GAGTGTTCCG ATATTGAGAA AGCAGTGGAG TTAATGTGCC TTGTCCCCAA GCGCTGTAAT 
GACATGATGA ATCTGGGACG CCTGCAGGGC TTCGAGGGCA CCCTGACCGC CCAGGGCAAG 
CTACTGCAGC AGGACACATT CTATGTGATC GAGCTGGATG CCGGCATGCA GTCCCGGACC 
AAGGAGAGGC GTGTGTTTCT CTTTGAGCAG ATTGTCATCT TCAGTGAACT GCTCCGAAAG 
GGGTCCCTCA CCCCAGGCTA CATGTTCAAA AGAAGCATCA AGATGAATTA CTTGGTCCTG 
GAAGACAATG TGGATGGTGA CCCCTGCAAA TTTGCACTCA TGAACAGGGA GACTTCAGAA 
AGGGTCATTC TACAGGCTGC CAACTCAGAC ATCCAGCAGG CCTGGGTGCA GGACATCAAC 
CAAGTCCTAG AAACACAGAG GGACTTCTTA AATGCACTAC AGTCACCCAT TGAGTATCAG 
CGGAAGGAGA GAAGCACAGC TGTGATCCGG TCCCAGCCCC CGAGGGTTCC ACAAGCCAGC 
CCCAGGCCCT ACTCTTCTGG CCCGGTGGGC TCAGAGAAGC CCCCCAAGGG CTCCAGCTAT 
AACCCGCCTC TGCCACCCCT GAAGATATCT ACCTCTAATG GCAGTCCAGG GTTCGACTAC 
CATCAGCCTG GGGACAAGTT TGACGCTAGC AAGCAGAACG ACCTGGGAGG CTGCAATGGG 
ACATCAACCA TGACTGTAAT CAAAGATTAC TATGCACTGA AGGAGAATGA AATCTGCGTG 
AGCCAAGGTG AGGTGGTACA GGTCCTCGCC GTCAACCAGC AGAACATGTG TCTGGTGTAC 
CAGCCTGCCA GCGACCATTC CCCGGCAGCA GAGGGCTGGG TCCCAGGCAG CATCCTGGCG 
CCCCTCGCCA AAGCCACAGC AGCGGCCGAA AGTAGTGACG GGAGCATCAA GAAGTCATGC 
TCTTGGCACA CTCTACGCAT GAGAAAGCGG GCAGACGTGG AGAACTCGGG TAAAAATGAA 
GCCACAGGGC CTCGTAAACC CAAGGATATT CTGGGCAACA AAGTCTCTGT TAAAGAGACG 
AACAGTTCCG AGGAGTCAGA GTGTGATGAT CTTGACCCTA ATACTAGTAT GGAGATCTTG 
AATCCAAATT TCATCCAGGA AGTGGCTCCA GAATTCCTCG TGCCCTTAGT AGATGTGACC 
TGTTTGCTTG GAGACACAGT GCTACTGCAG TGTAAAGCCT GTGGGCGGCC AAAGCCCAGC 
ATCACCTGGA AGGGTCCGGA TCAGAACATC CTAGACACTG ACAACAGTTC AGCAACATAC 
ACCATCTCCT CCTGTGATTC TGGGGAAAGC ACCCTCAAGA TCTGCAACCT GATGCCCCAG 
GATAGTGGGA TTTATACCTG CATAGCAGCA AACGACCACG GGACTGCATC CACATCTGCT 
ACAGTTAAGG TGCAAGGTGT CCCCGCAGCC CCCAACCGCC CAATCGCACA GGAGAGGAGC 
TGCACCTCCG TCATCCTCCG CTGGCTGCCC CCAGCCAGCA CCGGAAACTG CACTATTTCG 
GGGTACACAG TGGAGTATCG CGAGGAAGGT TCCCAGGTCT GGCAGCAATC CGTAGCTTCG 
ACCTTGGACA CTTACCTGGT CATTGAAGAC CTTAGTCCTG GGTGCCCTTA TCAGTTCAGG 
GTCAGTGCCA GCAACCCCTG GGGAATCAGC CTTCCCAGTG AGCCCTCGGA GTTTGTGCGC 
CTTCCAGAGT ACGATGCAGC CGCCGACGGT GCCACCATTT CTTGGAAGGA AAATTTTGAT 
TCTGCTTACA CTGAACTGAA CGAGATTGGC AGAGGCCGTT TCTCTATTGT AAAGAAATGC 
ATTCACAAAG CTACCCGCAA AGATGTTGCT GTGAAATTTG TGAGCAAAAA AATGAAGAAG 
AAGGAGCAGG CTGCTCACGA GGCCGCCCTG CTCCAGCACC TGCAGCACCC TCAGTATGTC 
ACGCTCCATG ACACCTACGA GTCCCCCACA TCCTACATCC TGATTTTGGA ACTGATGGAT 
GACGGCCGAC TCTTGGACTA CCTTATGAAT CATGATGAGC TGATGGAAGA AAAGGTAGCT 
TTCTACATTC GAGACATCAT GGAAGCTCTG CAGTACCTGC ACAACTGTAG GGTCGCACAT 
TTGGACATAA AGCCCGAAAA CCTGCTCATT GACCTCCGGA TCCCAGTGCC TCGAGTGAAG 
CTCATCGACT TGGAAGACGC TGTCCAGATC TCAGGCCACT TCCATATCCA CCATCTGCTG 
GGGAACCCTG AGTTCGCTGC CCCCGAAGTG ATCCAAGGCA TCCCTGTCTC CCTGGGCACA 
GACATCTGGA GCATCGGGGT TCTGACATAT GTCATGCTTA GTGGGGTCTC CCCCTTCTTA 
GATGAGAGCA AAGAGGAAAC CTGTATCAAC GTGTGCAGGG TGGACTTTAG CTTCCCCCAC 
GAGTACTTCT GCGGTGTGAG CAATGCTGCC AGGGATTTCA TCAACGTAAT CTTACAGGAA 
GACTTCCGGA GGCGGCCCAC AGCAGCCACG TGCCTGCAGC ATCCCTGGCT ACAGCCCCAC 
AACGGCAGCT ACTCCAAGAT CCCCTTGGAC ACCTCCCGTC TGGCCTGCTT CATAGAACGC 
CGCAAGCACC AGAATGATGT GCGGCCCATT CCCAATGTCA AGAGTTACAT CGTCAATAGG 
GTGAACCAAG GGACCTCGCT ATCCCACAAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522364.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522427.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X7, mRNA.

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CloneID	OMu52280
Clone ID Related Accession (Same CDS sequence)	XM_006522364.3 , XM_006522364.4
Accession Version	XM_006522364.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522364.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGAAG ACGCACCAGA GCGCCCTGCA GGTACAGCAG 
AAGGCAGAGG CACTGCTCCA GGCCGGCCAC TACGACGCAG ACGCCATCCG GGAATGTGCT 
GAGAAGGTGG CCCTCCACTG GCAACAGCTC ATGCTAAAGA TGGAGGACCG GCTGAAGCTT 
GTCAACGCCT CTGTGGCCTT TTACAAGACC TCTGAGCAGG TGTGCAGTGT TCTGGAGAGC 
TTGGAGCAGG AGTACCGGAG AGACGAGGAC TGGTGTGGTG GGAGAGACAA GCTGGGGCCG 
GCGGCAGAGA TGGACCACGT TATTCCACTC CTCAGCAAGC ACCTGGAGCA GAAGGAGGCC 
TTCCTGAAGG CCTGCACGCT GGCAAGGCGG AACGCCGAGG TGTTCCTCAA ATACATCCAC 
AGGAACAATG TGAGCATGCC TAGTGTTGCC AGCCACACCC GGGGGCCTGA ACAGCAAGTA 
AAAGCCATCT TGAGCGAGCT CTTGCAGAGG GAGAATCGAG TCCTGCACTT CTGGACCTTG 
AAGAAGCGGC GGTTAGACCA GTGTCAGCAA TATGTGGTGT TTGAGCGCAG TGCTAAGCAG 
GCACTGGACT GGATCCAAGA AACAGGAGAA TATTACCTCT CAACACATAC CTCCACCGGA 
GAGACCACGG AGGAGACTCA GGAGCTGCTG AAAGAGTATG GAGAATTCAG GGTGCCTGCC 
AAGCAAACGA AGGAGAAGGT GAAACTTCTG ATCCAGCTAG CAGACAGCTT TGTGGAAAAG 
GGCCACATCC ACGCCACAGA GATACGGAAA TGGGTGACCA CGGTGGACAA GCACTACCGC 
GATTTCTCCC TGAGGATGGG GAAGTACCGG TACTCGCTGG AGAAGGCGCT GGGGGTCAAC 
ACGGAGGATA ACAAGGACCT AGAGCTGGAC ATCATCCCAG CAAGCCTTTC GGATCGGGAG 
GTCAAGCTCC GGGACGCTAA CCATGAGATC AATGAAGAGA AACGGAAGTC AGCTCGAAAG 
AAAGAGTTCA TTATGGCTGA ACTGCTCCAA ACTGAGAAGG CTTATGTAAG GGACTTGCAC 
GAGTGCTTGG AGACCTACCT GTGGGAGATG ACTAGTGGTG TGGAAGAGAT ACCCCCCGGC 
ATCCTCAATA AGGAACATAT CATCTTCGGC AACATCCAGG AGATCTATGA CTTCCATAAC 
AACATCTTCC TCAAAGAGCT GGAGAAGTAC GAGCAGCTGC CTGAGGATGT GGGACACTGC 
TTTGTTACCT GGGCAGACAA ATTTCAGATG TATGTCACCT ACTGTAAAAA CAAGCCTGAT 
TCCAACCAGC TGATCCTGGA GCATGCGGGC ACCTTCTTTG ATGAGATCCA GCAGCGGCAT 
GGCCTGGCCA ACTCCATCTC CTCCTACCTA ATTAAGCCTG TCCAGAGGGT TACCAAGTAC 
CAGCTGCTCC TGAAGGAACT TCTAACTTGC TGTGAAGAAG GGAAAGGAGA GCTGAAGGAT 
GGCCTGGAGG TGATGCTCAG TGTCCCCAAG AAAGCCAACG ATGCCATGCA TGTCAGCATG 
CTGGAAGGGT TCGATGAAAA CTTGGATGTA CAGGGGGAGT TGATTCTCCA GGATGCCTTT 
CAAGTGTGGG ACCCAAAGTC GCTGATCCGG AAGGGGCGGG AGCGCCATCT GTTCCTCTTT 
GAGATCTCCT TGGTGTTTAG CAAAGAGATC AAAGACTCTT CAGGACACAC GAAATATGTT 
TACAAGAACA AGCTACTGAC CTCAGAGCTG GGTGTGACCG AGCATGTAGA AGGTGACCCC 
TGCAAATTCG CCTTGTGGTC TGGGCGCACC CCATCCTCAG ACAATAAAAC AGTGCTCAAA 
GCTTCCAACA TCGAAACCAA GCAGGAATGG ATCAAGAACA TTCGAGAAGT GATTCAGGAG 
AGGATCATTC ATCTTAAAGG CGCTTTAAAG GAGCCAATTC AGCTCCCCAA AACACCAGCC 
AAACTGAGGA ACAATAGTAA GAGGGATGGG GTAGAGGATG GTGACAGCCA AGGGGATGGG 
AGCAGCCAGC CAGATACCAT ATCCATTGCC TCCAGGACCT CTCAGAACAC AGTGGAAAGT 
GACAAGCTCT CAGGTGGATG TGAGCTGACC GTGGTCCTCC AGGACTTCAG CGCAGGCCAC 
AGCAGCGAGC TGAGCATCCA GGTGGGCCAG ACGGTGGAGC TGCTGGAACG ACCGAGTGAG 
AGGCCTGGCT GGTGTCTGGT CCGCACCACA GAGCGGAGCC CCCCACAGGA AGGCCTGGTA 
CCCAGCAGCG CCCTGTGCAT CTCACACTCT CGCAGCAGCG TGGAAATGGA CTGCTTCTTC 
CCCTTGAAAG ATTCATACTC TCATTCCTCC AGTGAGAATG GAGGCAAATC CGAGTCCGTG 
GCCCACCTGC AGTCTCAGCC CTCCCTGAAC TCCATCCACA GTTCCCCTGG TCCCAAGCGC 
TCCACCAACA CCCTCAAAAA GTGGCTGACC AGCCCCGTGC GCCGCCTCAA CAGCGGAAAA 
GCCGACGGGA ACATCAAAAA GCAGAAGAAA GTCCGGGATG GGCGGAAGAG CTTCGATCTG 
GGATCGCCCA AGCCTGGGGA TGAGACGACA CCTCAGGGAG ACAGCGCTGA CGAGAAGAGC 
AAGAAGGGTT GGGGCGAAGA CGAGCCAGAT GAAGAGTCAC ACACACCCCT CCCTCCGCCC 
ATGAAGATCT TTGACAACGA CCCCACACAG GATGAGATGT CGTCTTTGCT AGCAGCCCGG 
CAGGCTCCCC CTGACGTGCC TACTGCTGCA GACCTTGTCA GTGCAATAGA AAAGTTGGTC 
AAAAATAAGC TGACTCTGGA AGGAGGCTCT TACCGGGGGA GCTTGAAGGA CCCTACAGGC 
TGCCTGAATG AGGGGATGAC CCCTCCCACA CCTCCTAGAA ACCTGGAAGA AGAACAGAAA 
GCCAAGGCGC TGAGAGGCAG GATGTTTGTC CTGAATGAGC TGGTACAGAC AGAGAAGGAC 
TACGTCAAGG ACCTGGGGAT TGTGGTGGAG GGCTTCATGA AGAGAATAGA AGAGAAGGGG 
GTCCCTGAGG ACATGCGAGG GAAGGAAAAG ATCGTGTTTG GAAATATCCA CCAGATTTAC 
GACTGGCATA AAGATTTTTT CCTGGCTGAA CTGGAAAAAT GTATCCAAGA GCAAGACAGA 
TTGGCCCAGC TCTTCATTAA ACACGAGCGG AAGCTGCACA TCTACGTGTG GTACTGCCAG 
AACAAGCCGC GTTCCGAGTA CATCGTGGCT GAGTACGATG CCTATTTTGA GGAGGTTAAA 
CAAGAGATCA ATCAAAGGCT GACCCTTAGT GACTTCCTGA TCAAGCCCAT TCAGAGAATA 
ACAAAGTACC AGCTGCTACT CAAGGACTTC CTGCGATACA GCGAGAAGGC TGGTTTGGAG 
TGTTCCGATA TTGAGAAAGC AGTGGAGTTA ATGTGCCTTG TCCCCAAGCG CTGTAATGAC 
ATGATGAATC TGGGACGCCT GCAGGGCTTC GAGGGCACCC TGACCGCCCA GGGCAAGCTA 
CTGCAGCAGG ACACATTCTA TGTGATCGAG CTGGATGCCG GCATGCAGTC CCGGACCAAG 
GAGAGGCGTG TGTTTCTCTT TGAGCAGATT GTCATCTTCA GTGAACTGCT CCGAAAGGGG 
TCCCTCACCC CAGGCTACAT GTTCAAAAGA AGCATCAAGA TGAATTACTT GGTCCTGGAA 
GACAATGTGG ATGGTGACCC CTGCAAATTT GCACTCATGA ACAGGGAGAC TTCAGAAAGG 
GTCATTCTAC AGGCTGCCAA CTCAGACATC CAGCAGGCCT GGGTGCAGGA CATCAACCAA 
GTCCTAGAAA CACAGAGGGA CTTCTTAAAT GCACTACAGT CACCCATTGA GTATCAGCGG 
AAGGAGAGAA GCACAGCTGT GATCCGGTCC CAGCCCCCGA GGGTTCCACA AGCCAGCCCC 
AGGCCCTACT CTTCTGGCCC GGTGGGCTCA GAGAAGCCCC CCAAGGGCTC CAGCTATAAC 
CCGCCTCTGC CACCCCTGAA GATATCTACC TCTAATGGCA GTCCAGGGTT CGACTACCAT 
CAGCCTGGGG ACAAGTTTGA CGCTAGCAAG CAGAACGACC TGGGAGGCTG CAATGGGACA 
TCAACCATGA CTGTAATCAA AGATTACTAT GCACTGAAGG AGAATGAAAT CTGCGTGAGC 
CAAGGTGAGG TGGTACAGGT CCTCGCCGTC AACCAGCAGA ACATGTGTCT GGTGTACCAG 
CCTGCCAGCG ACCATTCCCC GGCAGCAGAG GGCTGGGTCC CAGGCAGCAT CCTGGCGCCC 
CTCGCCAAAG CCACAGCAGC GGCCGAAAGT AGTGACGGGA GCATCAAGAA GTCATGCTCT 
TGGCACACTC TACGCATGAG AAAGCGGGCA GACGTGGAGA ACTCGGGTAA AAATGAAGCC 
ACAGGGCCTC GTAAACCCAA GGATATTCTG GGCAACAAAG TCTCTGTTAA AGAGACGAAC 
AGTTCCGAGG AGTCAGAGTG TGATGATCTT GACCCTAATA CTAGTATGGA GATCTTGAAT 
CCAAATTTCA TCCAGGAAGT GGCTCCAGAA TTCCTCGTGC CCTTAGTAGA TGTGACCTGT 
TTGCTTGGAG ACACAGTGCT ACTGCAGTGT AAAGCCTGTG GGCGGCCAAA GCCCAGCATC 
ACCTGGAAGG GTCCGGATCA GAACATCCTA GACACTGACA ACAGTTCAGC AACATACACC 
ATCTCCTCCT GTGATTCTGG GGAAAGCACC CTCAAGATCT GCAACCTGAT GCCCCAGGAT 
AGTGGGATTT ATACCTGCAT AGCAGCAAAC GACCACGGGA CTGCATCCAC ATCTGCTACA 
GTTAAGGTGC AAGGTGTCCC CGCAGCCCCC AACCGCCCAA TCGCACAGGA GAGGAGCTGC 
ACCTCCGTCA TCCTCCGCTG GCTGCCCCCA GCCAGCACCG GAAACTGCAC TATTTCGGGG 
TACACAGTGG AGTATCGCGA GGAAGGTTCC CAGGTCTGGC AGCAATCCGT AGCTTCGACC 
TTGGACACTT ACCTGGTCAT TGAAGACCTT AGTCCTGGGT GCCCTTATCA GTTCAGGGTC 
AGTGCCAGCA ACCCCTGGGG AATCAGCCTT CCCAGTGAGC CCTCGGAGTT TGTGCGCCTT 
CCAGAGTACG ATGCAGCCGC CGACGGTGCC ACCATTTCTT GGAAGGAAAA TTTTGATTCT 
GCTTACACTG AACTGAACGA GATTGGCAGA GGCCGTTTCT CTATTGTAAA GAAATGCATT 
CACAAAGCTA CCCGCAAAGA TGTTGCTGTG AAATTTGTGA GCAAAAAAAT GAAGAAGAAG 
GAGCAGGCTG CTCACGAGGC CGCCCTGCTC CAGCACCTGC AGCACCCTCA GTATGTCACG 
CTCCATGACA CCTACGAGTC CCCCACATCC TACATCCTGA TTTTGGAACT GATGGATGAC 
GGCCGACTCT TGGACTACCT TATGAATCAT GATGAGCTGA TGGAAGAAAA GGTAGCTTTC 
TACATTCGAG ACATCATGGA AGCTCTGCAG TACCTGCACA ACTGTAGGGT CGCACATTTG 
GACATAAAGC CCGAAAACCT GCTCATTGAC CTCCGGATCC CAGTGCCTCG AGTGAAGCTC 
ATCGACTTGG AAGACGCTGT CCAGATCTCA GGCCACTTCC ATATCCACCA TCTGCTGGGG 
AACCCTGAGT TCGCTGCCCC CGAAGTGATC CAAGGCATCC CTGTCTCCCT GGGCACAGAC 
ATCTGGAGCA TCGGGGTTCT GACATATGTC ATGCTTAGTG GGGTCTCCCC CTTCTTAGAT 
GAGAGCAAAG AGGAAACCTG TATCAACGTG TGCAGGGTGG ACTTTAGCTT CCCCCACGAG 
TACTTCTGCG GTGTGAGCAA TGCTGCCAGG GATTTCATCA ACGTAATCTT ACAGGAAGAC 
TTCCGGAGGC GGCCCACAGC AGCCACGTGC CTGCAGCATC CCTGGCTACA GCCCCACAAC 
GGCAGCTACT CCAAGATCCC CTTGGACACC TCCCGTCTGG CCTGCTTCAT AGAACGCCGC 
AAGCACCAGA ATGATGTGCG GCCCATTCCC AATGTCAAGA GTTACATCGT CAATAGGGTG 
AACCAAGGGA CCTCGCTATC CCACAACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522427.1
CDS	4891..13863
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_006522364.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522364.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGAAG ACGCACCAGA GCGCCCTGCA GGTACAGCAG 
AAGGCAGAGG CACTGCTCCA GGCCGGCCAC TACGACGCAG ACGCCATCCG GGAATGTGCT 
GAGAAGGTGG CCCTCCACTG GCAACAGCTC ATGCTAAAGA TGGAGGACCG GCTGAAGCTT 
GTCAACGCCT CTGTGGCCTT TTACAAGACC TCTGAGCAGG TGTGCAGTGT TCTGGAGAGC 
TTGGAGCAGG AGTACCGGAG AGACGAGGAC TGGTGTGGTG GGAGAGACAA GCTGGGGCCG 
GCGGCAGAGA TGGACCACGT TATTCCACTC CTCAGCAAGC ACCTGGAGCA GAAGGAGGCC 
TTCCTGAAGG CCTGCACGCT GGCAAGGCGG AACGCCGAGG TGTTCCTCAA ATACATCCAC 
AGGAACAATG TGAGCATGCC TAGTGTTGCC AGCCACACCC GGGGGCCTGA ACAGCAAGTA 
AAAGCCATCT TGAGCGAGCT CTTGCAGAGG GAGAATCGAG TCCTGCACTT CTGGACCTTG 
AAGAAGCGGC GGTTAGACCA GTGTCAGCAA TATGTGGTGT TTGAGCGCAG TGCTAAGCAG 
GCACTGGACT GGATCCAAGA AACAGGAGAA TATTACCTCT CAACACATAC CTCCACCGGA 
GAGACCACGG AGGAGACTCA GGAGCTGCTG AAAGAGTATG GAGAATTCAG GGTGCCTGCC 
AAGCAAACGA AGGAGAAGGT GAAACTTCTG ATCCAGCTAG CAGACAGCTT TGTGGAAAAG 
GGCCACATCC ACGCCACAGA GATACGGAAA TGGGTGACCA CGGTGGACAA GCACTACCGC 
GATTTCTCCC TGAGGATGGG GAAGTACCGG TACTCGCTGG AGAAGGCGCT GGGGGTCAAC 
ACGGAGGATA ACAAGGACCT AGAGCTGGAC ATCATCCCAG CAAGCCTTTC GGATCGGGAG 
GTCAAGCTCC GGGACGCTAA CCATGAGATC AATGAAGAGA AACGGAAGTC AGCTCGAAAG 
AAAGAGTTCA TTATGGCTGA ACTGCTCCAA ACTGAGAAGG CTTATGTAAG GGACTTGCAC 
GAGTGCTTGG AGACCTACCT GTGGGAGATG ACTAGTGGTG TGGAAGAGAT ACCCCCCGGC 
ATCCTCAATA AGGAACATAT CATCTTCGGC AACATCCAGG AGATCTATGA CTTCCATAAC 
AACATCTTCC TCAAAGAGCT GGAGAAGTAC GAGCAGCTGC CTGAGGATGT GGGACACTGC 
TTTGTTACCT GGGCAGACAA ATTTCAGATG TATGTCACCT ACTGTAAAAA CAAGCCTGAT 
TCCAACCAGC TGATCCTGGA GCATGCGGGC ACCTTCTTTG ATGAGATCCA GCAGCGGCAT 
GGCCTGGCCA ACTCCATCTC CTCCTACCTA ATTAAGCCTG TCCAGAGGGT TACCAAGTAC 
CAGCTGCTCC TGAAGGAACT TCTAACTTGC TGTGAAGAAG GGAAAGGAGA GCTGAAGGAT 
GGCCTGGAGG TGATGCTCAG TGTCCCCAAG AAAGCCAACG ATGCCATGCA TGTCAGCATG 
CTGGAAGGGT TCGATGAAAA CTTGGATGTA CAGGGGGAGT TGATTCTCCA GGATGCCTTT 
CAAGTGTGGG ACCCAAAGTC GCTGATCCGG AAGGGGCGGG AGCGCCATCT GTTCCTCTTT 
GAGATCTCCT TGGTGTTTAG CAAAGAGATC AAAGACTCTT CAGGACACAC GAAATATGTT 
TACAAGAACA AGCTACTGAC CTCAGAGCTG GGTGTGACCG AGCATGTAGA AGGTGACCCC 
TGCAAATTCG CCTTGTGGTC TGGGCGCACC CCATCCTCAG ACAATAAAAC AGTGCTCAAA 
GCTTCCAACA TCGAAACCAA GCAGGAATGG ATCAAGAACA TTCGAGAAGT GATTCAGGAG 
AGGATCATTC ATCTTAAAGG CGCTTTAAAG GAGCCAATTC AGCTCCCCAA AACACCAGCC 
AAACTGAGGA ACAATAGTAA GAGGGATGGG GTAGAGGATG GTGACAGCCA AGGGGATGGG 
AGCAGCCAGC CAGATACCAT ATCCATTGCC TCCAGGACCT CTCAGAACAC AGTGGAAAGT 
GACAAGCTCT CAGGTGGATG TGAGCTGACC GTGGTCCTCC AGGACTTCAG CGCAGGCCAC 
AGCAGCGAGC TGAGCATCCA GGTGGGCCAG ACGGTGGAGC TGCTGGAACG ACCGAGTGAG 
AGGCCTGGCT GGTGTCTGGT CCGCACCACA GAGCGGAGCC CCCCACAGGA AGGCCTGGTA 
CCCAGCAGCG CCCTGTGCAT CTCACACTCT CGCAGCAGCG TGGAAATGGA CTGCTTCTTC 
CCCTTGAAAG ATTCATACTC TCATTCCTCC AGTGAGAATG GAGGCAAATC CGAGTCCGTG 
GCCCACCTGC AGTCTCAGCC CTCCCTGAAC TCCATCCACA GTTCCCCTGG TCCCAAGCGC 
TCCACCAACA CCCTCAAAAA GTGGCTGACC AGCCCCGTGC GCCGCCTCAA CAGCGGAAAA 
GCCGACGGGA ACATCAAAAA GCAGAAGAAA GTCCGGGATG GGCGGAAGAG CTTCGATCTG 
GGATCGCCCA AGCCTGGGGA TGAGACGACA CCTCAGGGAG ACAGCGCTGA CGAGAAGAGC 
AAGAAGGGTT GGGGCGAAGA CGAGCCAGAT GAAGAGTCAC ACACACCCCT CCCTCCGCCC 
ATGAAGATCT TTGACAACGA CCCCACACAG GATGAGATGT CGTCTTTGCT AGCAGCCCGG 
CAGGCTCCCC CTGACGTGCC TACTGCTGCA GACCTTGTCA GTGCAATAGA AAAGTTGGTC 
AAAAATAAGC TGACTCTGGA AGGAGGCTCT TACCGGGGGA GCTTGAAGGA CCCTACAGGC 
TGCCTGAATG AGGGGATGAC CCCTCCCACA CCTCCTAGAA ACCTGGAAGA AGAACAGAAA 
GCCAAGGCGC TGAGAGGCAG GATGTTTGTC CTGAATGAGC TGGTACAGAC AGAGAAGGAC 
TACGTCAAGG ACCTGGGGAT TGTGGTGGAG GGCTTCATGA AGAGAATAGA AGAGAAGGGG 
GTCCCTGAGG ACATGCGAGG GAAGGAAAAG ATCGTGTTTG GAAATATCCA CCAGATTTAC 
GACTGGCATA AAGATTTTTT CCTGGCTGAA CTGGAAAAAT GTATCCAAGA GCAAGACAGA 
TTGGCCCAGC TCTTCATTAA ACACGAGCGG AAGCTGCACA TCTACGTGTG GTACTGCCAG 
AACAAGCCGC GTTCCGAGTA CATCGTGGCT GAGTACGATG CCTATTTTGA GGAGGTTAAA 
CAAGAGATCA ATCAAAGGCT GACCCTTAGT GACTTCCTGA TCAAGCCCAT TCAGAGAATA 
ACAAAGTACC AGCTGCTACT CAAGGACTTC CTGCGATACA GCGAGAAGGC TGGTTTGGAG 
TGTTCCGATA TTGAGAAAGC AGTGGAGTTA ATGTGCCTTG TCCCCAAGCG CTGTAATGAC 
ATGATGAATC TGGGACGCCT GCAGGGCTTC GAGGGCACCC TGACCGCCCA GGGCAAGCTA 
CTGCAGCAGG ACACATTCTA TGTGATCGAG CTGGATGCCG GCATGCAGTC CCGGACCAAG 
GAGAGGCGTG TGTTTCTCTT TGAGCAGATT GTCATCTTCA GTGAACTGCT CCGAAAGGGG 
TCCCTCACCC CAGGCTACAT GTTCAAAAGA AGCATCAAGA TGAATTACTT GGTCCTGGAA 
GACAATGTGG ATGGTGACCC CTGCAAATTT GCACTCATGA ACAGGGAGAC TTCAGAAAGG 
GTCATTCTAC AGGCTGCCAA CTCAGACATC CAGCAGGCCT GGGTGCAGGA CATCAACCAA 
GTCCTAGAAA CACAGAGGGA CTTCTTAAAT GCACTACAGT CACCCATTGA GTATCAGCGG 
AAGGAGAGAA GCACAGCTGT GATCCGGTCC CAGCCCCCGA GGGTTCCACA AGCCAGCCCC 
AGGCCCTACT CTTCTGGCCC GGTGGGCTCA GAGAAGCCCC CCAAGGGCTC CAGCTATAAC 
CCGCCTCTGC CACCCCTGAA GATATCTACC TCTAATGGCA GTCCAGGGTT CGACTACCAT 
CAGCCTGGGG ACAAGTTTGA CGCTAGCAAG CAGAACGACC TGGGAGGCTG CAATGGGACA 
TCAACCATGA CTGTAATCAA AGATTACTAT GCACTGAAGG AGAATGAAAT CTGCGTGAGC 
CAAGGTGAGG TGGTACAGGT CCTCGCCGTC AACCAGCAGA ACATGTGTCT GGTGTACCAG 
CCTGCCAGCG ACCATTCCCC GGCAGCAGAG GGCTGGGTCC CAGGCAGCAT CCTGGCGCCC 
CTCGCCAAAG CCACAGCAGC GGCCGAAAGT AGTGACGGGA GCATCAAGAA GTCATGCTCT 
TGGCACACTC TACGCATGAG AAAGCGGGCA GACGTGGAGA ACTCGGGTAA AAATGAAGCC 
ACAGGGCCTC GTAAACCCAA GGATATTCTG GGCAACAAAG TCTCTGTTAA AGAGACGAAC 
AGTTCCGAGG AGTCAGAGTG TGATGATCTT GACCCTAATA CTAGTATGGA GATCTTGAAT 
CCAAATTTCA TCCAGGAAGT GGCTCCAGAA TTCCTCGTGC CCTTAGTAGA TGTGACCTGT 
TTGCTTGGAG ACACAGTGCT ACTGCAGTGT AAAGCCTGTG GGCGGCCAAA GCCCAGCATC 
ACCTGGAAGG GTCCGGATCA GAACATCCTA GACACTGACA ACAGTTCAGC AACATACACC 
ATCTCCTCCT GTGATTCTGG GGAAAGCACC CTCAAGATCT GCAACCTGAT GCCCCAGGAT 
AGTGGGATTT ATACCTGCAT AGCAGCAAAC GACCACGGGA CTGCATCCAC ATCTGCTACA 
GTTAAGGTGC AAGGTGTCCC CGCAGCCCCC AACCGCCCAA TCGCACAGGA GAGGAGCTGC 
ACCTCCGTCA TCCTCCGCTG GCTGCCCCCA GCCAGCACCG GAAACTGCAC TATTTCGGGG 
TACACAGTGG AGTATCGCGA GGAAGGTTCC CAGGTCTGGC AGCAATCCGT AGCTTCGACC 
TTGGACACTT ACCTGGTCAT TGAAGACCTT AGTCCTGGGT GCCCTTATCA GTTCAGGGTC 
AGTGCCAGCA ACCCCTGGGG AATCAGCCTT CCCAGTGAGC CCTCGGAGTT TGTGCGCCTT 
CCAGAGTACG ATGCAGCCGC CGACGGTGCC ACCATTTCTT GGAAGGAAAA TTTTGATTCT 
GCTTACACTG AACTGAACGA GATTGGCAGA GGCCGTTTCT CTATTGTAAA GAAATGCATT 
CACAAAGCTA CCCGCAAAGA TGTTGCTGTG AAATTTGTGA GCAAAAAAAT GAAGAAGAAG 
GAGCAGGCTG CTCACGAGGC CGCCCTGCTC CAGCACCTGC AGCACCCTCA GTATGTCACG 
CTCCATGACA CCTACGAGTC CCCCACATCC TACATCCTGA TTTTGGAACT GATGGATGAC 
GGCCGACTCT TGGACTACCT TATGAATCAT GATGAGCTGA TGGAAGAAAA GGTAGCTTTC 
TACATTCGAG ACATCATGGA AGCTCTGCAG TACCTGCACA ACTGTAGGGT CGCACATTTG 
GACATAAAGC CCGAAAACCT GCTCATTGAC CTCCGGATCC CAGTGCCTCG AGTGAAGCTC 
ATCGACTTGG AAGACGCTGT CCAGATCTCA GGCCACTTCC ATATCCACCA TCTGCTGGGG 
AACCCTGAGT TCGCTGCCCC CGAAGTGATC CAAGGCATCC CTGTCTCCCT GGGCACAGAC 
ATCTGGAGCA TCGGGGTTCT GACATATGTC ATGCTTAGTG GGGTCTCCCC CTTCTTAGAT 
GAGAGCAAAG AGGAAACCTG TATCAACGTG TGCAGGGTGG ACTTTAGCTT CCCCCACGAG 
TACTTCTGCG GTGTGAGCAA TGCTGCCAGG GATTTCATCA ACGTAATCTT ACAGGAAGAC 
TTCCGGAGGC GGCCCACAGC AGCCACGTGC CTGCAGCATC CCTGGCTACA GCCCCACAAC 
GGCAGCTACT CCAAGATCCC CTTGGACACC TCCCGTCTGG CCTGCTTCAT AGAACGCCGC 
AAGCACCAGA ATGATGTGCG GCCCATTCCC AATGTCAAGA GTTACATCGT CAATAGGGTG 
AACCAAGGGA CCTCGCTATC CCACAACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522364.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522427.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X7, mRNA.

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CloneID	OMu52280
Clone ID Related Accession (Same CDS sequence)	XM_006522364.3 , XM_006522364.4
Accession Version	XM_006522364.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522364.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGAAG ACGCACCAGA GCGCCCTGCA GGTACAGCAG 
AAGGCAGAGG CACTGCTCCA GGCCGGCCAC TACGACGCAG ACGCCATCCG GGAATGTGCT 
GAGAAGGTGG CCCTCCACTG GCAACAGCTC ATGCTAAAGA TGGAGGACCG GCTGAAGCTT 
GTCAACGCCT CTGTGGCCTT TTACAAGACC TCTGAGCAGG TGTGCAGTGT TCTGGAGAGC 
TTGGAGCAGG AGTACCGGAG AGACGAGGAC TGGTGTGGTG GGAGAGACAA GCTGGGGCCG 
GCGGCAGAGA TGGACCACGT TATTCCACTC CTCAGCAAGC ACCTGGAGCA GAAGGAGGCC 
TTCCTGAAGG CCTGCACGCT GGCAAGGCGG AACGCCGAGG TGTTCCTCAA ATACATCCAC 
AGGAACAATG TGAGCATGCC TAGTGTTGCC AGCCACACCC GGGGGCCTGA ACAGCAAGTA 
AAAGCCATCT TGAGCGAGCT CTTGCAGAGG GAGAATCGAG TCCTGCACTT CTGGACCTTG 
AAGAAGCGGC GGTTAGACCA GTGTCAGCAA TATGTGGTGT TTGAGCGCAG TGCTAAGCAG 
GCACTGGACT GGATCCAAGA AACAGGAGAA TATTACCTCT CAACACATAC CTCCACCGGA 
GAGACCACGG AGGAGACTCA GGAGCTGCTG AAAGAGTATG GAGAATTCAG GGTGCCTGCC 
AAGCAAACGA AGGAGAAGGT GAAACTTCTG ATCCAGCTAG CAGACAGCTT TGTGGAAAAG 
GGCCACATCC ACGCCACAGA GATACGGAAA TGGGTGACCA CGGTGGACAA GCACTACCGC 
GATTTCTCCC TGAGGATGGG GAAGTACCGG TACTCGCTGG AGAAGGCGCT GGGGGTCAAC 
ACGGAGGATA ACAAGGACCT AGAGCTGGAC ATCATCCCAG CAAGCCTTTC GGATCGGGAG 
GTCAAGCTCC GGGACGCTAA CCATGAGATC AATGAAGAGA AACGGAAGTC AGCTCGAAAG 
AAAGAGTTCA TTATGGCTGA ACTGCTCCAA ACTGAGAAGG CTTATGTAAG GGACTTGCAC 
GAGTGCTTGG AGACCTACCT GTGGGAGATG ACTAGTGGTG TGGAAGAGAT ACCCCCCGGC 
ATCCTCAATA AGGAACATAT CATCTTCGGC AACATCCAGG AGATCTATGA CTTCCATAAC 
AACATCTTCC TCAAAGAGCT GGAGAAGTAC GAGCAGCTGC CTGAGGATGT GGGACACTGC 
TTTGTTACCT GGGCAGACAA ATTTCAGATG TATGTCACCT ACTGTAAAAA CAAGCCTGAT 
TCCAACCAGC TGATCCTGGA GCATGCGGGC ACCTTCTTTG ATGAGATCCA GCAGCGGCAT 
GGCCTGGCCA ACTCCATCTC CTCCTACCTA ATTAAGCCTG TCCAGAGGGT TACCAAGTAC 
CAGCTGCTCC TGAAGGAACT TCTAACTTGC TGTGAAGAAG GGAAAGGAGA GCTGAAGGAT 
GGCCTGGAGG TGATGCTCAG TGTCCCCAAG AAAGCCAACG ATGCCATGCA TGTCAGCATG 
CTGGAAGGGT TCGATGAAAA CTTGGATGTA CAGGGGGAGT TGATTCTCCA GGATGCCTTT 
CAAGTGTGGG ACCCAAAGTC GCTGATCCGG AAGGGGCGGG AGCGCCATCT GTTCCTCTTT 
GAGATCTCCT TGGTGTTTAG CAAAGAGATC AAAGACTCTT CAGGACACAC GAAATATGTT 
TACAAGAACA AGCTACTGAC CTCAGAGCTG GGTGTGACCG AGCATGTAGA AGGTGACCCC 
TGCAAATTCG CCTTGTGGTC TGGGCGCACC CCATCCTCAG ACAATAAAAC AGTGCTCAAA 
GCTTCCAACA TCGAAACCAA GCAGGAATGG ATCAAGAACA TTCGAGAAGT GATTCAGGAG 
AGGATCATTC ATCTTAAAGG CGCTTTAAAG GAGCCAATTC AGCTCCCCAA AACACCAGCC 
AAACTGAGGA ACAATAGTAA GAGGGATGGG GTAGAGGATG GTGACAGCCA AGGGGATGGG 
AGCAGCCAGC CAGATACCAT ATCCATTGCC TCCAGGACCT CTCAGAACAC AGTGGAAAGT 
GACAAGCTCT CAGGTGGATG TGAGCTGACC GTGGTCCTCC AGGACTTCAG CGCAGGCCAC 
AGCAGCGAGC TGAGCATCCA GGTGGGCCAG ACGGTGGAGC TGCTGGAACG ACCGAGTGAG 
AGGCCTGGCT GGTGTCTGGT CCGCACCACA GAGCGGAGCC CCCCACAGGA AGGCCTGGTA 
CCCAGCAGCG CCCTGTGCAT CTCACACTCT CGCAGCAGCG TGGAAATGGA CTGCTTCTTC 
CCCTTGAAAG ATTCATACTC TCATTCCTCC AGTGAGAATG GAGGCAAATC CGAGTCCGTG 
GCCCACCTGC AGTCTCAGCC CTCCCTGAAC TCCATCCACA GTTCCCCTGG TCCCAAGCGC 
TCCACCAACA CCCTCAAAAA GTGGCTGACC AGCCCCGTGC GCCGCCTCAA CAGCGGAAAA 
GCCGACGGGA ACATCAAAAA GCAGAAGAAA GTCCGGGATG GGCGGAAGAG CTTCGATCTG 
GGATCGCCCA AGCCTGGGGA TGAGACGACA CCTCAGGGAG ACAGCGCTGA CGAGAAGAGC 
AAGAAGGGTT GGGGCGAAGA CGAGCCAGAT GAAGAGTCAC ACACACCCCT CCCTCCGCCC 
ATGAAGATCT TTGACAACGA CCCCACACAG GATGAGATGT CGTCTTTGCT AGCAGCCCGG 
CAGGCTCCCC CTGACGTGCC TACTGCTGCA GACCTTGTCA GTGCAATAGA AAAGTTGGTC 
AAAAATAAGC TGACTCTGGA AGGAGGCTCT TACCGGGGGA GCTTGAAGGA CCCTACAGGC 
TGCCTGAATG AGGGGATGAC CCCTCCCACA CCTCCTAGAA ACCTGGAAGA AGAACAGAAA 
GCCAAGGCGC TGAGAGGCAG GATGTTTGTC CTGAATGAGC TGGTACAGAC AGAGAAGGAC 
TACGTCAAGG ACCTGGGGAT TGTGGTGGAG GGCTTCATGA AGAGAATAGA AGAGAAGGGG 
GTCCCTGAGG ACATGCGAGG GAAGGAAAAG ATCGTGTTTG GAAATATCCA CCAGATTTAC 
GACTGGCATA AAGATTTTTT CCTGGCTGAA CTGGAAAAAT GTATCCAAGA GCAAGACAGA 
TTGGCCCAGC TCTTCATTAA ACACGAGCGG AAGCTGCACA TCTACGTGTG GTACTGCCAG 
AACAAGCCGC GTTCCGAGTA CATCGTGGCT GAGTACGATG CCTATTTTGA GGAGGTTAAA 
CAAGAGATCA ATCAAAGGCT GACCCTTAGT GACTTCCTGA TCAAGCCCAT TCAGAGAATA 
ACAAAGTACC AGCTGCTACT CAAGGACTTC CTGCGATACA GCGAGAAGGC TGGTTTGGAG 
TGTTCCGATA TTGAGAAAGC AGTGGAGTTA ATGTGCCTTG TCCCCAAGCG CTGTAATGAC 
ATGATGAATC TGGGACGCCT GCAGGGCTTC GAGGGCACCC TGACCGCCCA GGGCAAGCTA 
CTGCAGCAGG ACACATTCTA TGTGATCGAG CTGGATGCCG GCATGCAGTC CCGGACCAAG 
GAGAGGCGTG TGTTTCTCTT TGAGCAGATT GTCATCTTCA GTGAACTGCT CCGAAAGGGG 
TCCCTCACCC CAGGCTACAT GTTCAAAAGA AGCATCAAGA TGAATTACTT GGTCCTGGAA 
GACAATGTGG ATGGTGACCC CTGCAAATTT GCACTCATGA ACAGGGAGAC TTCAGAAAGG 
GTCATTCTAC AGGCTGCCAA CTCAGACATC CAGCAGGCCT GGGTGCAGGA CATCAACCAA 
GTCCTAGAAA CACAGAGGGA CTTCTTAAAT GCACTACAGT CACCCATTGA GTATCAGCGG 
AAGGAGAGAA GCACAGCTGT GATCCGGTCC CAGCCCCCGA GGGTTCCACA AGCCAGCCCC 
AGGCCCTACT CTTCTGGCCC GGTGGGCTCA GAGAAGCCCC CCAAGGGCTC CAGCTATAAC 
CCGCCTCTGC CACCCCTGAA GATATCTACC TCTAATGGCA GTCCAGGGTT CGACTACCAT 
CAGCCTGGGG ACAAGTTTGA CGCTAGCAAG CAGAACGACC TGGGAGGCTG CAATGGGACA 
TCAACCATGA CTGTAATCAA AGATTACTAT GCACTGAAGG AGAATGAAAT CTGCGTGAGC 
CAAGGTGAGG TGGTACAGGT CCTCGCCGTC AACCAGCAGA ACATGTGTCT GGTGTACCAG 
CCTGCCAGCG ACCATTCCCC GGCAGCAGAG GGCTGGGTCC CAGGCAGCAT CCTGGCGCCC 
CTCGCCAAAG CCACAGCAGC GGCCGAAAGT AGTGACGGGA GCATCAAGAA GTCATGCTCT 
TGGCACACTC TACGCATGAG AAAGCGGGCA GACGTGGAGA ACTCGGGTAA AAATGAAGCC 
ACAGGGCCTC GTAAACCCAA GGATATTCTG GGCAACAAAG TCTCTGTTAA AGAGACGAAC 
AGTTCCGAGG AGTCAGAGTG TGATGATCTT GACCCTAATA CTAGTATGGA GATCTTGAAT 
CCAAATTTCA TCCAGGAAGT GGCTCCAGAA TTCCTCGTGC CCTTAGTAGA TGTGACCTGT 
TTGCTTGGAG ACACAGTGCT ACTGCAGTGT AAAGCCTGTG GGCGGCCAAA GCCCAGCATC 
ACCTGGAAGG GTCCGGATCA GAACATCCTA GACACTGACA ACAGTTCAGC AACATACACC 
ATCTCCTCCT GTGATTCTGG GGAAAGCACC CTCAAGATCT GCAACCTGAT GCCCCAGGAT 
AGTGGGATTT ATACCTGCAT AGCAGCAAAC GACCACGGGA CTGCATCCAC ATCTGCTACA 
GTTAAGGTGC AAGGTGTCCC CGCAGCCCCC AACCGCCCAA TCGCACAGGA GAGGAGCTGC 
ACCTCCGTCA TCCTCCGCTG GCTGCCCCCA GCCAGCACCG GAAACTGCAC TATTTCGGGG 
TACACAGTGG AGTATCGCGA GGAAGGTTCC CAGGTCTGGC AGCAATCCGT AGCTTCGACC 
TTGGACACTT ACCTGGTCAT TGAAGACCTT AGTCCTGGGT GCCCTTATCA GTTCAGGGTC 
AGTGCCAGCA ACCCCTGGGG AATCAGCCTT CCCAGTGAGC CCTCGGAGTT TGTGCGCCTT 
CCAGAGTACG ATGCAGCCGC CGACGGTGCC ACCATTTCTT GGAAGGAAAA TTTTGATTCT 
GCTTACACTG AACTGAACGA GATTGGCAGA GGCCGTTTCT CTATTGTAAA GAAATGCATT 
CACAAAGCTA CCCGCAAAGA TGTTGCTGTG AAATTTGTGA GCAAAAAAAT GAAGAAGAAG 
GAGCAGGCTG CTCACGAGGC CGCCCTGCTC CAGCACCTGC AGCACCCTCA GTATGTCACG 
CTCCATGACA CCTACGAGTC CCCCACATCC TACATCCTGA TTTTGGAACT GATGGATGAC 
GGCCGACTCT TGGACTACCT TATGAATCAT GATGAGCTGA TGGAAGAAAA GGTAGCTTTC 
TACATTCGAG ACATCATGGA AGCTCTGCAG TACCTGCACA ACTGTAGGGT CGCACATTTG 
GACATAAAGC CCGAAAACCT GCTCATTGAC CTCCGGATCC CAGTGCCTCG AGTGAAGCTC 
ATCGACTTGG AAGACGCTGT CCAGATCTCA GGCCACTTCC ATATCCACCA TCTGCTGGGG 
AACCCTGAGT TCGCTGCCCC CGAAGTGATC CAAGGCATCC CTGTCTCCCT GGGCACAGAC 
ATCTGGAGCA TCGGGGTTCT GACATATGTC ATGCTTAGTG GGGTCTCCCC CTTCTTAGAT 
GAGAGCAAAG AGGAAACCTG TATCAACGTG TGCAGGGTGG ACTTTAGCTT CCCCCACGAG 
TACTTCTGCG GTGTGAGCAA TGCTGCCAGG GATTTCATCA ACGTAATCTT ACAGGAAGAC 
TTCCGGAGGC GGCCCACAGC AGCCACGTGC CTGCAGCATC CCTGGCTACA GCCCCACAAC 
GGCAGCTACT CCAAGATCCC CTTGGACACC TCCCGTCTGG CCTGCTTCAT AGAACGCCGC 
AAGCACCAGA ATGATGTGCG GCCCATTCCC AATGTCAAGA GTTACATCGT CAATAGGGTG 
AACCAAGGGA CCTCGCTATC CCACAACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522427.1
CDS	24875..33847
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_006522364.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522364.4

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGAAG ACGCACCAGA GCGCCCTGCA GGTACAGCAG 
AAGGCAGAGG CACTGCTCCA GGCCGGCCAC TACGACGCAG ACGCCATCCG GGAATGTGCT 
GAGAAGGTGG CCCTCCACTG GCAACAGCTC ATGCTAAAGA TGGAGGACCG GCTGAAGCTT 
GTCAACGCCT CTGTGGCCTT TTACAAGACC TCTGAGCAGG TGTGCAGTGT TCTGGAGAGC 
TTGGAGCAGG AGTACCGGAG AGACGAGGAC TGGTGTGGTG GGAGAGACAA GCTGGGGCCG 
GCGGCAGAGA TGGACCACGT TATTCCACTC CTCAGCAAGC ACCTGGAGCA GAAGGAGGCC 
TTCCTGAAGG CCTGCACGCT GGCAAGGCGG AACGCCGAGG TGTTCCTCAA ATACATCCAC 
AGGAACAATG TGAGCATGCC TAGTGTTGCC AGCCACACCC GGGGGCCTGA ACAGCAAGTA 
AAAGCCATCT TGAGCGAGCT CTTGCAGAGG GAGAATCGAG TCCTGCACTT CTGGACCTTG 
AAGAAGCGGC GGTTAGACCA GTGTCAGCAA TATGTGGTGT TTGAGCGCAG TGCTAAGCAG 
GCACTGGACT GGATCCAAGA AACAGGAGAA TATTACCTCT CAACACATAC CTCCACCGGA 
GAGACCACGG AGGAGACTCA GGAGCTGCTG AAAGAGTATG GAGAATTCAG GGTGCCTGCC 
AAGCAAACGA AGGAGAAGGT GAAACTTCTG ATCCAGCTAG CAGACAGCTT TGTGGAAAAG 
GGCCACATCC ACGCCACAGA GATACGGAAA TGGGTGACCA CGGTGGACAA GCACTACCGC 
GATTTCTCCC TGAGGATGGG GAAGTACCGG TACTCGCTGG AGAAGGCGCT GGGGGTCAAC 
ACGGAGGATA ACAAGGACCT AGAGCTGGAC ATCATCCCAG CAAGCCTTTC GGATCGGGAG 
GTCAAGCTCC GGGACGCTAA CCATGAGATC AATGAAGAGA AACGGAAGTC AGCTCGAAAG 
AAAGAGTTCA TTATGGCTGA ACTGCTCCAA ACTGAGAAGG CTTATGTAAG GGACTTGCAC 
GAGTGCTTGG AGACCTACCT GTGGGAGATG ACTAGTGGTG TGGAAGAGAT ACCCCCCGGC 
ATCCTCAATA AGGAACATAT CATCTTCGGC AACATCCAGG AGATCTATGA CTTCCATAAC 
AACATCTTCC TCAAAGAGCT GGAGAAGTAC GAGCAGCTGC CTGAGGATGT GGGACACTGC 
TTTGTTACCT GGGCAGACAA ATTTCAGATG TATGTCACCT ACTGTAAAAA CAAGCCTGAT 
TCCAACCAGC TGATCCTGGA GCATGCGGGC ACCTTCTTTG ATGAGATCCA GCAGCGGCAT 
GGCCTGGCCA ACTCCATCTC CTCCTACCTA ATTAAGCCTG TCCAGAGGGT TACCAAGTAC 
CAGCTGCTCC TGAAGGAACT TCTAACTTGC TGTGAAGAAG GGAAAGGAGA GCTGAAGGAT 
GGCCTGGAGG TGATGCTCAG TGTCCCCAAG AAAGCCAACG ATGCCATGCA TGTCAGCATG 
CTGGAAGGGT TCGATGAAAA CTTGGATGTA CAGGGGGAGT TGATTCTCCA GGATGCCTTT 
CAAGTGTGGG ACCCAAAGTC GCTGATCCGG AAGGGGCGGG AGCGCCATCT GTTCCTCTTT 
GAGATCTCCT TGGTGTTTAG CAAAGAGATC AAAGACTCTT CAGGACACAC GAAATATGTT 
TACAAGAACA AGCTACTGAC CTCAGAGCTG GGTGTGACCG AGCATGTAGA AGGTGACCCC 
TGCAAATTCG CCTTGTGGTC TGGGCGCACC CCATCCTCAG ACAATAAAAC AGTGCTCAAA 
GCTTCCAACA TCGAAACCAA GCAGGAATGG ATCAAGAACA TTCGAGAAGT GATTCAGGAG 
AGGATCATTC ATCTTAAAGG CGCTTTAAAG GAGCCAATTC AGCTCCCCAA AACACCAGCC 
AAACTGAGGA ACAATAGTAA GAGGGATGGG GTAGAGGATG GTGACAGCCA AGGGGATGGG 
AGCAGCCAGC CAGATACCAT ATCCATTGCC TCCAGGACCT CTCAGAACAC AGTGGAAAGT 
GACAAGCTCT CAGGTGGATG TGAGCTGACC GTGGTCCTCC AGGACTTCAG CGCAGGCCAC 
AGCAGCGAGC TGAGCATCCA GGTGGGCCAG ACGGTGGAGC TGCTGGAACG ACCGAGTGAG 
AGGCCTGGCT GGTGTCTGGT CCGCACCACA GAGCGGAGCC CCCCACAGGA AGGCCTGGTA 
CCCAGCAGCG CCCTGTGCAT CTCACACTCT CGCAGCAGCG TGGAAATGGA CTGCTTCTTC 
CCCTTGAAAG ATTCATACTC TCATTCCTCC AGTGAGAATG GAGGCAAATC CGAGTCCGTG 
GCCCACCTGC AGTCTCAGCC CTCCCTGAAC TCCATCCACA GTTCCCCTGG TCCCAAGCGC 
TCCACCAACA CCCTCAAAAA GTGGCTGACC AGCCCCGTGC GCCGCCTCAA CAGCGGAAAA 
GCCGACGGGA ACATCAAAAA GCAGAAGAAA GTCCGGGATG GGCGGAAGAG CTTCGATCTG 
GGATCGCCCA AGCCTGGGGA TGAGACGACA CCTCAGGGAG ACAGCGCTGA CGAGAAGAGC 
AAGAAGGGTT GGGGCGAAGA CGAGCCAGAT GAAGAGTCAC ACACACCCCT CCCTCCGCCC 
ATGAAGATCT TTGACAACGA CCCCACACAG GATGAGATGT CGTCTTTGCT AGCAGCCCGG 
CAGGCTCCCC CTGACGTGCC TACTGCTGCA GACCTTGTCA GTGCAATAGA AAAGTTGGTC 
AAAAATAAGC TGACTCTGGA AGGAGGCTCT TACCGGGGGA GCTTGAAGGA CCCTACAGGC 
TGCCTGAATG AGGGGATGAC CCCTCCCACA CCTCCTAGAA ACCTGGAAGA AGAACAGAAA 
GCCAAGGCGC TGAGAGGCAG GATGTTTGTC CTGAATGAGC TGGTACAGAC AGAGAAGGAC 
TACGTCAAGG ACCTGGGGAT TGTGGTGGAG GGCTTCATGA AGAGAATAGA AGAGAAGGGG 
GTCCCTGAGG ACATGCGAGG GAAGGAAAAG ATCGTGTTTG GAAATATCCA CCAGATTTAC 
GACTGGCATA AAGATTTTTT CCTGGCTGAA CTGGAAAAAT GTATCCAAGA GCAAGACAGA 
TTGGCCCAGC TCTTCATTAA ACACGAGCGG AAGCTGCACA TCTACGTGTG GTACTGCCAG 
AACAAGCCGC GTTCCGAGTA CATCGTGGCT GAGTACGATG CCTATTTTGA GGAGGTTAAA 
CAAGAGATCA ATCAAAGGCT GACCCTTAGT GACTTCCTGA TCAAGCCCAT TCAGAGAATA 
ACAAAGTACC AGCTGCTACT CAAGGACTTC CTGCGATACA GCGAGAAGGC TGGTTTGGAG 
TGTTCCGATA TTGAGAAAGC AGTGGAGTTA ATGTGCCTTG TCCCCAAGCG CTGTAATGAC 
ATGATGAATC TGGGACGCCT GCAGGGCTTC GAGGGCACCC TGACCGCCCA GGGCAAGCTA 
CTGCAGCAGG ACACATTCTA TGTGATCGAG CTGGATGCCG GCATGCAGTC CCGGACCAAG 
GAGAGGCGTG TGTTTCTCTT TGAGCAGATT GTCATCTTCA GTGAACTGCT CCGAAAGGGG 
TCCCTCACCC CAGGCTACAT GTTCAAAAGA AGCATCAAGA TGAATTACTT GGTCCTGGAA 
GACAATGTGG ATGGTGACCC CTGCAAATTT GCACTCATGA ACAGGGAGAC TTCAGAAAGG 
GTCATTCTAC AGGCTGCCAA CTCAGACATC CAGCAGGCCT GGGTGCAGGA CATCAACCAA 
GTCCTAGAAA CACAGAGGGA CTTCTTAAAT GCACTACAGT CACCCATTGA GTATCAGCGG 
AAGGAGAGAA GCACAGCTGT GATCCGGTCC CAGCCCCCGA GGGTTCCACA AGCCAGCCCC 
AGGCCCTACT CTTCTGGCCC GGTGGGCTCA GAGAAGCCCC CCAAGGGCTC CAGCTATAAC 
CCGCCTCTGC CACCCCTGAA GATATCTACC TCTAATGGCA GTCCAGGGTT CGACTACCAT 
CAGCCTGGGG ACAAGTTTGA CGCTAGCAAG CAGAACGACC TGGGAGGCTG CAATGGGACA 
TCAACCATGA CTGTAATCAA AGATTACTAT GCACTGAAGG AGAATGAAAT CTGCGTGAGC 
CAAGGTGAGG TGGTACAGGT CCTCGCCGTC AACCAGCAGA ACATGTGTCT GGTGTACCAG 
CCTGCCAGCG ACCATTCCCC GGCAGCAGAG GGCTGGGTCC CAGGCAGCAT CCTGGCGCCC 
CTCGCCAAAG CCACAGCAGC GGCCGAAAGT AGTGACGGGA GCATCAAGAA GTCATGCTCT 
TGGCACACTC TACGCATGAG AAAGCGGGCA GACGTGGAGA ACTCGGGTAA AAATGAAGCC 
ACAGGGCCTC GTAAACCCAA GGATATTCTG GGCAACAAAG TCTCTGTTAA AGAGACGAAC 
AGTTCCGAGG AGTCAGAGTG TGATGATCTT GACCCTAATA CTAGTATGGA GATCTTGAAT 
CCAAATTTCA TCCAGGAAGT GGCTCCAGAA TTCCTCGTGC CCTTAGTAGA TGTGACCTGT 
TTGCTTGGAG ACACAGTGCT ACTGCAGTGT AAAGCCTGTG GGCGGCCAAA GCCCAGCATC 
ACCTGGAAGG GTCCGGATCA GAACATCCTA GACACTGACA ACAGTTCAGC AACATACACC 
ATCTCCTCCT GTGATTCTGG GGAAAGCACC CTCAAGATCT GCAACCTGAT GCCCCAGGAT 
AGTGGGATTT ATACCTGCAT AGCAGCAAAC GACCACGGGA CTGCATCCAC ATCTGCTACA 
GTTAAGGTGC AAGGTGTCCC CGCAGCCCCC AACCGCCCAA TCGCACAGGA GAGGAGCTGC 
ACCTCCGTCA TCCTCCGCTG GCTGCCCCCA GCCAGCACCG GAAACTGCAC TATTTCGGGG 
TACACAGTGG AGTATCGCGA GGAAGGTTCC CAGGTCTGGC AGCAATCCGT AGCTTCGACC 
TTGGACACTT ACCTGGTCAT TGAAGACCTT AGTCCTGGGT GCCCTTATCA GTTCAGGGTC 
AGTGCCAGCA ACCCCTGGGG AATCAGCCTT CCCAGTGAGC CCTCGGAGTT TGTGCGCCTT 
CCAGAGTACG ATGCAGCCGC CGACGGTGCC ACCATTTCTT GGAAGGAAAA TTTTGATTCT 
GCTTACACTG AACTGAACGA GATTGGCAGA GGCCGTTTCT CTATTGTAAA GAAATGCATT 
CACAAAGCTA CCCGCAAAGA TGTTGCTGTG AAATTTGTGA GCAAAAAAAT GAAGAAGAAG 
GAGCAGGCTG CTCACGAGGC CGCCCTGCTC CAGCACCTGC AGCACCCTCA GTATGTCACG 
CTCCATGACA CCTACGAGTC CCCCACATCC TACATCCTGA TTTTGGAACT GATGGATGAC 
GGCCGACTCT TGGACTACCT TATGAATCAT GATGAGCTGA TGGAAGAAAA GGTAGCTTTC 
TACATTCGAG ACATCATGGA AGCTCTGCAG TACCTGCACA ACTGTAGGGT CGCACATTTG 
GACATAAAGC CCGAAAACCT GCTCATTGAC CTCCGGATCC CAGTGCCTCG AGTGAAGCTC 
ATCGACTTGG AAGACGCTGT CCAGATCTCA GGCCACTTCC ATATCCACCA TCTGCTGGGG 
AACCCTGAGT TCGCTGCCCC CGAAGTGATC CAAGGCATCC CTGTCTCCCT GGGCACAGAC 
ATCTGGAGCA TCGGGGTTCT GACATATGTC ATGCTTAGTG GGGTCTCCCC CTTCTTAGAT 
GAGAGCAAAG AGGAAACCTG TATCAACGTG TGCAGGGTGG ACTTTAGCTT CCCCCACGAG 
TACTTCTGCG GTGTGAGCAA TGCTGCCAGG GATTTCATCA ACGTAATCTT ACAGGAAGAC 
TTCCGGAGGC GGCCCACAGC AGCCACGTGC CTGCAGCATC CCTGGCTACA GCCCCACAAC 
GGCAGCTACT CCAAGATCCC CTTGGACACC TCCCGTCTGG CCTGCTTCAT AGAACGCCGC 
AAGCACCAGA ATGATGTGCG GCCCATTCCC AATGTCAAGA GTTACATCGT CAATAGGGTG 
AACCAAGGGA CCTCGCTATC CCACAACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522370.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522433.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X10, mRNA.

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CloneID	OMu52286
Clone ID Related Accession (Same CDS sequence)	XM_006522370.3 , XM_006522370.4
Accession Version	XM_006522370.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522370.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGAA GACGCACCAG 
AGCGCCCTGC AGGTACAGCA GAAGGCAGAG GCACTGCTCC AGGCCGGCCA CTACGACGCA 
GACGCCATCC GGGAATGTGC TGAGAAGGTG GCCCTCCACT GGCAACAGCT CATGCTAAAG 
ATGGAGGACC GGCTGAAGCT TGTCAACGCC TCTGTGGCCT TTTACAAGAC CTCTGAGCAG 
GTGTGCAGTG TTCTGGAGAG CTTGGAGCAG GAGTACCGGA GAGACGAGGA CTGGTGTGGT 
GGGAGAGACA AGCTGGGGCC GGCGGCAGAG ATGGACCACG TTATTCCACT CCTCAGCAAG 
CACCTGGAGC AGAAGGAGGC CTTCCTGAAG GCCTGCACGC TGGCAAGGCG GAACGCCGAG 
GTGTTCCTCA AATACATCCA CAGGAACAAT GTGAGCATGC CTAGTGTTGC CAGCCACACC 
CGGGGGCCTG AACAGCAAGT AAAAGCCATC TTGAGCGAGC TCTTGCAGAG GGAGAATCGA 
GTCCTGCACT TCTGGACCTT GAAGAAGCGG CGGTTAGACC AGTGTCAGCA ATATGTGGTG 
TTTGAGCGCA GTGCTAAGCA GGCACTGGAC TGGATCCAAG AAACAGGAGA ATATTACCTC 
TCAACACATA CCTCCACCGG AGAGACCACG GAGGAGACTC AGGAGCTGCT GAAAGAGTAT 
GGAGAATTCA GGGTGCCTGC CAAGCAAACG AAGGAGAAGG TGAAACTTCT GATCCAGCTA 
GCAGACAGCT TTGTGGAAAA GGGCCACATC CACGCCACAG AGATACGGAA ATGGGTGACC 
ACGGTGGACA AGCACTACCG CGATTTCTCC CTGAGGATGG GGAAGTACCG GTACTCGCTG 
GAGAAGGCGC TGGGGGTCAA CACGGAGGAT AACAAGGACC TAGAGCTGGA CATCATCCCA 
GCAAGCCTTT CGGATCGGGA GGTCAAGCTC CGGGACGCTA ACCATGAGAT CAATGAAGAG 
AAACGGAAGT CAGCTCGAAA GAAAGAGTTC ATTATGGCTG AACTGCTCCA AACTGAGAAG 
GCTTATGTAA GGGACTTGCA CGAGTGCTTG GAGACCTACC TGTGGGAGAT GACTAGTGGT 
GTGGAAGAGA TACCCCCCGG CATCCTCAAT AAGGAACATA TCATCTTCGG CAACATCCAG 
GAGATCTATG ACTTCCATAA CAACATCTTC CTCAAAGAGC TGGAGAAGTA CGAGCAGCTG 
CCTGAGGATG TGGGACACTG CTTTGTTACC TGGGCAGACA AATTTCAGAT GTATGTCACC 
TACTGTAAAA ACAAGCCTGA TTCCAACCAG CTGATCCTGG AGCATGCGGG CACCTTCTTT 
GATGAGATCC AGCAGCGGCA TGGCCTGGCC AACTCCATCT CCTCCTACCT AATTAAGCCT 
GTCCAGAGGG TTACCAAGTA CCAGCTGCTC CTGAAGGAAC TTCTAACTTG CTGTGAAGAA 
GGGAAAGGAG AGCTGAAGGA TGGCCTGGAG GTGATGCTCA GTGTCCCCAA GAAAGCCAAC 
GATGCCATGC ATGTCAGCAT GCTGGAAGGG TTCGATGAAA ACTTGGATGT ACAGGGGGAG 
TTGATTCTCC AGGATGCCTT TCAAGTGTGG GACCCAAAGT CGCTGATCCG GAAGGGGCGG 
GAGCGCCATC TGTTCCTCTT TGAGATCTCC TTGGTGTTTA GCAAAGAGAT CAAAGACTCT 
TCAGGACACA CGAAATATGT TTACAAGAAC AAGCTACTGA CCTCAGAGCT GGGTGTGACC 
GAGCATGTAG AAGGTGACCC CTGCAAATTC GCCTTGTGGT CTGGGCGCAC CCCATCCTCA 
GACAATAAAA CAGTGCTCAA AGCTTCCAAC ATCGAAACCA AGCAGGAATG GATCAAGAAC 
ATTCGAGAAG TGATTCAGGA GAGGATCATT CATCTTAAAG GCGCTTTAAA GGAGCCAATT 
CAGCTCCCCA AAACACCAGC CAAACTGAGG AACAATAGTA AGAGGGATGG GGTAGAGGAT 
GGTGACAGCC AAGGGGATGG GAGCAGCCAG CCAGATACCA TATCCATTGC CTCCAGGACC 
TCTCAGAACA CAGTGGAAAG TGACAAGCTC TCAGGTGGAT GTGAGCTGAC CGTGGTCCTC 
CAGGACTTCA GCGCAGGCCA CAGCAGCGAG CTGAGCATCC AGGTGGGCCA GACGGTGGAG 
CTGCTGGAAC GACCGAGTGA GAGGCCTGGC TGGTGTCTGG TCCGCACCAC AGAGCGGAGC 
CCCCCACAGG AAGGCCTGGT ACCCAGCAGC GCCCTGTGCA TCTCACACTC TCGCAGCAGC 
GTGGAAATGG ACTGCTTCTT CCCCTTGAAA GATTCATACT CTCATTCCTC CAGTGAGAAT 
GGAGGCAAAT CCGAGTCCGT GGCCCACCTG CAGTCTCAGC CCTCCCTGAA CTCCATCCAC 
AGTTCCCCTG GTCCCAAGCG CTCCACCAAC ACCCTCAAAA AGTGGCTGAC CAGCCCCGTG 
CGCCGCCTCA ACAGCGGAAA AGCCGACGGG AACATCAAAA AGCAGAAGAA AGTCCGGGAT 
GGGCGGAAGA GCTTCGATCT GGGATCGCCC AAGCCTGGGG ATGAGACGAC ACCTCAGGGA 
GACAGCGCTG ACGAGAAGAG CAAGAAGGGT TGGGGCGAAG ACGAGCCAGA TGAAGAGTCA 
CACACACCCC TCCCTCCGCC CATGAAGATC TTTGACAACG ACCCCACACA GGATGAGATG 
TCGTCTTTGC TAGCAGCCCG GCAGGCTCCC CCTGACGTGC CTACTGCTGC AGACCTTGTC 
AGTGCAATAG AAAAGTTGGT CAAAAATAAG CTGACTCTGG AAGGAGGCTC TTACCGGGGG 
AGCTTGAAGG ACCCTACAGG CTGCCTGAAT GAGGGGATGA CCCCTCCCAC ACCTCCTAGA 
AACCTGGAAG AAGAACAGAA AGCCAAGGCG CTGAGAGGCA GGATGTTTGT CCTGAATGAG 
CTGGTACAGA CAGAGAAGGA CTACGTCAAG GACCTGGGGA TTGTGGTGGA GGGCTTCATG 
AAGAGAATAG AAGAGAAGGG GGTCCCTGAG GACATGCGAG GGAAGGAAAA GATCGTGTTT 
GGAAATATCC ACCAGATTTA CGACTGGCAT AAAGATTTTT TCCTGGCTGA ACTGGAAAAA 
TGTATCCAAG AGCAAGACAG ATTGGCCCAG CTCTTCATTA AACACGAGCG GAAGCTGCAC 
ATCTACGTGT GGTACTGCCA GAACAAGCCG CGTTCCGAGT ACATCGTGGC TGAGTACGAT 
GCCTATTTTG AGGAGGTTAA ACAAGAGATC AATCAAAGGC TGACCCTTAG TGACTTCCTG 
ATCAAGCCCA TTCAGAGAAT AACAAAGTAC CAGCTGCTAC TCAAGGACTT CCTGCGATAC 
AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT ATTGAGAAAG CAGTGGAGTT AATGTGCCTT 
GTCCCCAAGC GCTGTAATGA CATGATGAAT CTGGGACGCC TGCAGGGCTT CGAGGGCACC 
CTGACCGCCC AGGGCAAGCT ACTGCAGCAG GACACATTCT ATGTGATCGA GCTGGATGCC 
GGCATGCAGT CCCGGACCAA GGAGAGGCGT GTGTTTCTCT TTGAGCAGAT TGTCATCTTC 
AGTGAACTGC TCCGAAAGGG GTCCCTCACC CCAGGCTACA TGTTCAAAAG AAGCATCAAG 
ATGAATTACT TGGTCCTGGA AGACAATGTG GATGGTGACC CCTGCAAATT TGCACTCATG 
AACAGGGAGA CTTCAGAAAG GGTCATTCTA CAGGCTGCCA ACTCAGACAT CCAGCAGGCC 
TGGGTGCAGG ACATCAACCA AGTCCTAGAA ACACAGAGGG ACTTCTTAAA TGCACTACAG 
TCACCCATTG AGTATCAGCG GAAGGAGAGA AGCACAGCTG TGATCCGGTC CCAGCCCCCG 
AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC 
CCCAAGGGCT CCAGCTATAA CCCGCCTCTG CCACCCCTGA AGATATCTAC CTCTAATGGC 
AGTCCAGGGT TCGACTACCA TCAGCCTGGG GACAAGTTTG ACGCTAGCAA GCAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAGA AGTCATGCTC TTGGCACACT CTACGCATGA GAAAGCGGGC AGACGTGGAG 
AACTCGGGTA AAAATGAAGC CACAGGGCCT CGTAAACCCA AGGATATTCT GGGCAACAAA 
GTCTCTGTTA AAGAGACGAA CAGTTCCGAG GAGTCAGAGT GTGATGATCT TGACCCTAAT 
ACTAGTATGG AGATCTTGAA TCCAAATTTC ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG 
CCCTTAGTAG ATGTGACCTG TTTGCTTGGA GACACAGTGC TACTGCAGTG TAAAGCCTGT 
GGGCGGCCAA AGCCCAGCAT CACCTGGAAG GGTCCGGATC AGAACATCCT AGACACTGAC 
AACAGTTCAG CAACATACAC CATCTCCTCC TGTGATTCTG GGGAAAGCAC CCTCAAGATC 
TGCAACCTGA TGCCCCAGGA TAGTGGGATT TATACCTGCA TAGCAGCAAA CGACCACGGG 
ACTGCATCCA CATCTGCTAC AGTTAAGGTG CAAGGTGTCC CCGCAGCCCC CAACCGCCCA 
ATCGCACAGG AGAGGAGCTG CACCTCCGTC ATCCTCCGCT GGCTGCCCCC AGCCAGCACC 
GGAAACTGCA CTATTTCGGG GTACACAGTG GAGTATCGCG AGGAAGGTTC CCAGGTCTGG 
CAGCAATCCG TAGCTTCGAC CTTGGACACT TACCTGGTCA TTGAAGACCT TAGTCCTGGG 
TGCCCTTATC AGTTCAGGGT CAGTGCCAGC AACCCCTGGG GAATCAGCCT TCCCAGTGAG 
CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC GATGCAGCCG CCGACGGTGC CACCATTTCT 
TGGAAGGAAA ATTTTGATTC TGCTTACACT GAACTGAACG AGATTGGCAG AGGCCGTTTC 
TCTATTGTAA AGAAATGCAT TCACAAAGCT ACCCGCAAAG ATGTTGCTGT GAAATTTGTG 
AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT GCTCACGAGG CCGCCCTGCT CCAGCACCTG 
CAGCACCCTC AGTATGTCAC GCTCCATGAC ACCTACGAGT CCCCCACATC CTACATCCTG 
ATTTTGGAAC TGATGGATGA CGGCCGACTC TTGGACTACC TTATGAATCA TGATGAGCTG 
ATGGAAGAAA AGGTAGCTTT CTACATTCGA GACATCATGG AAGCTCTGCA GTACCTGCAC 
AACTGTAGGG TCGCACATTT GGACATAAAG CCCGAAAACC TGCTCATTGA CCTCCGGATC 
CCAGTGCCTC GAGTGAAGCT CATCGACTTG GAAGACGCTG TCCAGATCTC AGGCCACTTC 
CATATCCACC ATCTGCTGGG GAACCCTGAG TTCGCTGCCC CCGAAGTGAT CCAAGGCATC 
CCTGTCTCCC TGGGCACAGA CATCTGGAGC ATCGGGGTTC TGACATATGT CATGCTTAGT 
GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA GAGGAAACCT GTATCAACGT GTGCAGGGTG 
GACTTTAGCT TCCCCCACGA GTACTTCTGC GGTGTGAGCA ATGCTGCCAG GGATTTCATC 
AACGTAATCT TACAGGAAGA CTTCCGGAGG CGGCCCACAG CAGCCACGTG CCTGCAGCAT 
CCCTGGCTAC AGCCCCACAA CGGCAGCTAC TCCAAGATCC CCTTGGACAC CTCCCGTCTG 
GCCTGCTTCA TAGAACGCCG CAAGCACCAG AATGATGTGC GGCCCATTCC CAATGTCAAG 
AGTTACATCG TCAATAGGGT GAACCAAGGG ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522433.1
CDS	4891..13824
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_006522370.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522370.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGAA GACGCACCAG 
AGCGCCCTGC AGGTACAGCA GAAGGCAGAG GCACTGCTCC AGGCCGGCCA CTACGACGCA 
GACGCCATCC GGGAATGTGC TGAGAAGGTG GCCCTCCACT GGCAACAGCT CATGCTAAAG 
ATGGAGGACC GGCTGAAGCT TGTCAACGCC TCTGTGGCCT TTTACAAGAC CTCTGAGCAG 
GTGTGCAGTG TTCTGGAGAG CTTGGAGCAG GAGTACCGGA GAGACGAGGA CTGGTGTGGT 
GGGAGAGACA AGCTGGGGCC GGCGGCAGAG ATGGACCACG TTATTCCACT CCTCAGCAAG 
CACCTGGAGC AGAAGGAGGC CTTCCTGAAG GCCTGCACGC TGGCAAGGCG GAACGCCGAG 
GTGTTCCTCA AATACATCCA CAGGAACAAT GTGAGCATGC CTAGTGTTGC CAGCCACACC 
CGGGGGCCTG AACAGCAAGT AAAAGCCATC TTGAGCGAGC TCTTGCAGAG GGAGAATCGA 
GTCCTGCACT TCTGGACCTT GAAGAAGCGG CGGTTAGACC AGTGTCAGCA ATATGTGGTG 
TTTGAGCGCA GTGCTAAGCA GGCACTGGAC TGGATCCAAG AAACAGGAGA ATATTACCTC 
TCAACACATA CCTCCACCGG AGAGACCACG GAGGAGACTC AGGAGCTGCT GAAAGAGTAT 
GGAGAATTCA GGGTGCCTGC CAAGCAAACG AAGGAGAAGG TGAAACTTCT GATCCAGCTA 
GCAGACAGCT TTGTGGAAAA GGGCCACATC CACGCCACAG AGATACGGAA ATGGGTGACC 
ACGGTGGACA AGCACTACCG CGATTTCTCC CTGAGGATGG GGAAGTACCG GTACTCGCTG 
GAGAAGGCGC TGGGGGTCAA CACGGAGGAT AACAAGGACC TAGAGCTGGA CATCATCCCA 
GCAAGCCTTT CGGATCGGGA GGTCAAGCTC CGGGACGCTA ACCATGAGAT CAATGAAGAG 
AAACGGAAGT CAGCTCGAAA GAAAGAGTTC ATTATGGCTG AACTGCTCCA AACTGAGAAG 
GCTTATGTAA GGGACTTGCA CGAGTGCTTG GAGACCTACC TGTGGGAGAT GACTAGTGGT 
GTGGAAGAGA TACCCCCCGG CATCCTCAAT AAGGAACATA TCATCTTCGG CAACATCCAG 
GAGATCTATG ACTTCCATAA CAACATCTTC CTCAAAGAGC TGGAGAAGTA CGAGCAGCTG 
CCTGAGGATG TGGGACACTG CTTTGTTACC TGGGCAGACA AATTTCAGAT GTATGTCACC 
TACTGTAAAA ACAAGCCTGA TTCCAACCAG CTGATCCTGG AGCATGCGGG CACCTTCTTT 
GATGAGATCC AGCAGCGGCA TGGCCTGGCC AACTCCATCT CCTCCTACCT AATTAAGCCT 
GTCCAGAGGG TTACCAAGTA CCAGCTGCTC CTGAAGGAAC TTCTAACTTG CTGTGAAGAA 
GGGAAAGGAG AGCTGAAGGA TGGCCTGGAG GTGATGCTCA GTGTCCCCAA GAAAGCCAAC 
GATGCCATGC ATGTCAGCAT GCTGGAAGGG TTCGATGAAA ACTTGGATGT ACAGGGGGAG 
TTGATTCTCC AGGATGCCTT TCAAGTGTGG GACCCAAAGT CGCTGATCCG GAAGGGGCGG 
GAGCGCCATC TGTTCCTCTT TGAGATCTCC TTGGTGTTTA GCAAAGAGAT CAAAGACTCT 
TCAGGACACA CGAAATATGT TTACAAGAAC AAGCTACTGA CCTCAGAGCT GGGTGTGACC 
GAGCATGTAG AAGGTGACCC CTGCAAATTC GCCTTGTGGT CTGGGCGCAC CCCATCCTCA 
GACAATAAAA CAGTGCTCAA AGCTTCCAAC ATCGAAACCA AGCAGGAATG GATCAAGAAC 
ATTCGAGAAG TGATTCAGGA GAGGATCATT CATCTTAAAG GCGCTTTAAA GGAGCCAATT 
CAGCTCCCCA AAACACCAGC CAAACTGAGG AACAATAGTA AGAGGGATGG GGTAGAGGAT 
GGTGACAGCC AAGGGGATGG GAGCAGCCAG CCAGATACCA TATCCATTGC CTCCAGGACC 
TCTCAGAACA CAGTGGAAAG TGACAAGCTC TCAGGTGGAT GTGAGCTGAC CGTGGTCCTC 
CAGGACTTCA GCGCAGGCCA CAGCAGCGAG CTGAGCATCC AGGTGGGCCA GACGGTGGAG 
CTGCTGGAAC GACCGAGTGA GAGGCCTGGC TGGTGTCTGG TCCGCACCAC AGAGCGGAGC 
CCCCCACAGG AAGGCCTGGT ACCCAGCAGC GCCCTGTGCA TCTCACACTC TCGCAGCAGC 
GTGGAAATGG ACTGCTTCTT CCCCTTGAAA GATTCATACT CTCATTCCTC CAGTGAGAAT 
GGAGGCAAAT CCGAGTCCGT GGCCCACCTG CAGTCTCAGC CCTCCCTGAA CTCCATCCAC 
AGTTCCCCTG GTCCCAAGCG CTCCACCAAC ACCCTCAAAA AGTGGCTGAC CAGCCCCGTG 
CGCCGCCTCA ACAGCGGAAA AGCCGACGGG AACATCAAAA AGCAGAAGAA AGTCCGGGAT 
GGGCGGAAGA GCTTCGATCT GGGATCGCCC AAGCCTGGGG ATGAGACGAC ACCTCAGGGA 
GACAGCGCTG ACGAGAAGAG CAAGAAGGGT TGGGGCGAAG ACGAGCCAGA TGAAGAGTCA 
CACACACCCC TCCCTCCGCC CATGAAGATC TTTGACAACG ACCCCACACA GGATGAGATG 
TCGTCTTTGC TAGCAGCCCG GCAGGCTCCC CCTGACGTGC CTACTGCTGC AGACCTTGTC 
AGTGCAATAG AAAAGTTGGT CAAAAATAAG CTGACTCTGG AAGGAGGCTC TTACCGGGGG 
AGCTTGAAGG ACCCTACAGG CTGCCTGAAT GAGGGGATGA CCCCTCCCAC ACCTCCTAGA 
AACCTGGAAG AAGAACAGAA AGCCAAGGCG CTGAGAGGCA GGATGTTTGT CCTGAATGAG 
CTGGTACAGA CAGAGAAGGA CTACGTCAAG GACCTGGGGA TTGTGGTGGA GGGCTTCATG 
AAGAGAATAG AAGAGAAGGG GGTCCCTGAG GACATGCGAG GGAAGGAAAA GATCGTGTTT 
GGAAATATCC ACCAGATTTA CGACTGGCAT AAAGATTTTT TCCTGGCTGA ACTGGAAAAA 
TGTATCCAAG AGCAAGACAG ATTGGCCCAG CTCTTCATTA AACACGAGCG GAAGCTGCAC 
ATCTACGTGT GGTACTGCCA GAACAAGCCG CGTTCCGAGT ACATCGTGGC TGAGTACGAT 
GCCTATTTTG AGGAGGTTAA ACAAGAGATC AATCAAAGGC TGACCCTTAG TGACTTCCTG 
ATCAAGCCCA TTCAGAGAAT AACAAAGTAC CAGCTGCTAC TCAAGGACTT CCTGCGATAC 
AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT ATTGAGAAAG CAGTGGAGTT AATGTGCCTT 
GTCCCCAAGC GCTGTAATGA CATGATGAAT CTGGGACGCC TGCAGGGCTT CGAGGGCACC 
CTGACCGCCC AGGGCAAGCT ACTGCAGCAG GACACATTCT ATGTGATCGA GCTGGATGCC 
GGCATGCAGT CCCGGACCAA GGAGAGGCGT GTGTTTCTCT TTGAGCAGAT TGTCATCTTC 
AGTGAACTGC TCCGAAAGGG GTCCCTCACC CCAGGCTACA TGTTCAAAAG AAGCATCAAG 
ATGAATTACT TGGTCCTGGA AGACAATGTG GATGGTGACC CCTGCAAATT TGCACTCATG 
AACAGGGAGA CTTCAGAAAG GGTCATTCTA CAGGCTGCCA ACTCAGACAT CCAGCAGGCC 
TGGGTGCAGG ACATCAACCA AGTCCTAGAA ACACAGAGGG ACTTCTTAAA TGCACTACAG 
TCACCCATTG AGTATCAGCG GAAGGAGAGA AGCACAGCTG TGATCCGGTC CCAGCCCCCG 
AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC 
CCCAAGGGCT CCAGCTATAA CCCGCCTCTG CCACCCCTGA AGATATCTAC CTCTAATGGC 
AGTCCAGGGT TCGACTACCA TCAGCCTGGG GACAAGTTTG ACGCTAGCAA GCAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAGA AGTCATGCTC TTGGCACACT CTACGCATGA GAAAGCGGGC AGACGTGGAG 
AACTCGGGTA AAAATGAAGC CACAGGGCCT CGTAAACCCA AGGATATTCT GGGCAACAAA 
GTCTCTGTTA AAGAGACGAA CAGTTCCGAG GAGTCAGAGT GTGATGATCT TGACCCTAAT 
ACTAGTATGG AGATCTTGAA TCCAAATTTC ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG 
CCCTTAGTAG ATGTGACCTG TTTGCTTGGA GACACAGTGC TACTGCAGTG TAAAGCCTGT 
GGGCGGCCAA AGCCCAGCAT CACCTGGAAG GGTCCGGATC AGAACATCCT AGACACTGAC 
AACAGTTCAG CAACATACAC CATCTCCTCC TGTGATTCTG GGGAAAGCAC CCTCAAGATC 
TGCAACCTGA TGCCCCAGGA TAGTGGGATT TATACCTGCA TAGCAGCAAA CGACCACGGG 
ACTGCATCCA CATCTGCTAC AGTTAAGGTG CAAGGTGTCC CCGCAGCCCC CAACCGCCCA 
ATCGCACAGG AGAGGAGCTG CACCTCCGTC ATCCTCCGCT GGCTGCCCCC AGCCAGCACC 
GGAAACTGCA CTATTTCGGG GTACACAGTG GAGTATCGCG AGGAAGGTTC CCAGGTCTGG 
CAGCAATCCG TAGCTTCGAC CTTGGACACT TACCTGGTCA TTGAAGACCT TAGTCCTGGG 
TGCCCTTATC AGTTCAGGGT CAGTGCCAGC AACCCCTGGG GAATCAGCCT TCCCAGTGAG 
CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC GATGCAGCCG CCGACGGTGC CACCATTTCT 
TGGAAGGAAA ATTTTGATTC TGCTTACACT GAACTGAACG AGATTGGCAG AGGCCGTTTC 
TCTATTGTAA AGAAATGCAT TCACAAAGCT ACCCGCAAAG ATGTTGCTGT GAAATTTGTG 
AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT GCTCACGAGG CCGCCCTGCT CCAGCACCTG 
CAGCACCCTC AGTATGTCAC GCTCCATGAC ACCTACGAGT CCCCCACATC CTACATCCTG 
ATTTTGGAAC TGATGGATGA CGGCCGACTC TTGGACTACC TTATGAATCA TGATGAGCTG 
ATGGAAGAAA AGGTAGCTTT CTACATTCGA GACATCATGG AAGCTCTGCA GTACCTGCAC 
AACTGTAGGG TCGCACATTT GGACATAAAG CCCGAAAACC TGCTCATTGA CCTCCGGATC 
CCAGTGCCTC GAGTGAAGCT CATCGACTTG GAAGACGCTG TCCAGATCTC AGGCCACTTC 
CATATCCACC ATCTGCTGGG GAACCCTGAG TTCGCTGCCC CCGAAGTGAT CCAAGGCATC 
CCTGTCTCCC TGGGCACAGA CATCTGGAGC ATCGGGGTTC TGACATATGT CATGCTTAGT 
GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA GAGGAAACCT GTATCAACGT GTGCAGGGTG 
GACTTTAGCT TCCCCCACGA GTACTTCTGC GGTGTGAGCA ATGCTGCCAG GGATTTCATC 
AACGTAATCT TACAGGAAGA CTTCCGGAGG CGGCCCACAG CAGCCACGTG CCTGCAGCAT 
CCCTGGCTAC AGCCCCACAA CGGCAGCTAC TCCAAGATCC CCTTGGACAC CTCCCGTCTG 
GCCTGCTTCA TAGAACGCCG CAAGCACCAG AATGATGTGC GGCCCATTCC CAATGTCAAG 
AGTTACATCG TCAATAGGGT GAACCAAGGG ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522370.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522433.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X10, mRNA.

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CloneID	OMu52286
Clone ID Related Accession (Same CDS sequence)	XM_006522370.3 , XM_006522370.4
Accession Version	XM_006522370.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522370.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGAA GACGCACCAG 
AGCGCCCTGC AGGTACAGCA GAAGGCAGAG GCACTGCTCC AGGCCGGCCA CTACGACGCA 
GACGCCATCC GGGAATGTGC TGAGAAGGTG GCCCTCCACT GGCAACAGCT CATGCTAAAG 
ATGGAGGACC GGCTGAAGCT TGTCAACGCC TCTGTGGCCT TTTACAAGAC CTCTGAGCAG 
GTGTGCAGTG TTCTGGAGAG CTTGGAGCAG GAGTACCGGA GAGACGAGGA CTGGTGTGGT 
GGGAGAGACA AGCTGGGGCC GGCGGCAGAG ATGGACCACG TTATTCCACT CCTCAGCAAG 
CACCTGGAGC AGAAGGAGGC CTTCCTGAAG GCCTGCACGC TGGCAAGGCG GAACGCCGAG 
GTGTTCCTCA AATACATCCA CAGGAACAAT GTGAGCATGC CTAGTGTTGC CAGCCACACC 
CGGGGGCCTG AACAGCAAGT AAAAGCCATC TTGAGCGAGC TCTTGCAGAG GGAGAATCGA 
GTCCTGCACT TCTGGACCTT GAAGAAGCGG CGGTTAGACC AGTGTCAGCA ATATGTGGTG 
TTTGAGCGCA GTGCTAAGCA GGCACTGGAC TGGATCCAAG AAACAGGAGA ATATTACCTC 
TCAACACATA CCTCCACCGG AGAGACCACG GAGGAGACTC AGGAGCTGCT GAAAGAGTAT 
GGAGAATTCA GGGTGCCTGC CAAGCAAACG AAGGAGAAGG TGAAACTTCT GATCCAGCTA 
GCAGACAGCT TTGTGGAAAA GGGCCACATC CACGCCACAG AGATACGGAA ATGGGTGACC 
ACGGTGGACA AGCACTACCG CGATTTCTCC CTGAGGATGG GGAAGTACCG GTACTCGCTG 
GAGAAGGCGC TGGGGGTCAA CACGGAGGAT AACAAGGACC TAGAGCTGGA CATCATCCCA 
GCAAGCCTTT CGGATCGGGA GGTCAAGCTC CGGGACGCTA ACCATGAGAT CAATGAAGAG 
AAACGGAAGT CAGCTCGAAA GAAAGAGTTC ATTATGGCTG AACTGCTCCA AACTGAGAAG 
GCTTATGTAA GGGACTTGCA CGAGTGCTTG GAGACCTACC TGTGGGAGAT GACTAGTGGT 
GTGGAAGAGA TACCCCCCGG CATCCTCAAT AAGGAACATA TCATCTTCGG CAACATCCAG 
GAGATCTATG ACTTCCATAA CAACATCTTC CTCAAAGAGC TGGAGAAGTA CGAGCAGCTG 
CCTGAGGATG TGGGACACTG CTTTGTTACC TGGGCAGACA AATTTCAGAT GTATGTCACC 
TACTGTAAAA ACAAGCCTGA TTCCAACCAG CTGATCCTGG AGCATGCGGG CACCTTCTTT 
GATGAGATCC AGCAGCGGCA TGGCCTGGCC AACTCCATCT CCTCCTACCT AATTAAGCCT 
GTCCAGAGGG TTACCAAGTA CCAGCTGCTC CTGAAGGAAC TTCTAACTTG CTGTGAAGAA 
GGGAAAGGAG AGCTGAAGGA TGGCCTGGAG GTGATGCTCA GTGTCCCCAA GAAAGCCAAC 
GATGCCATGC ATGTCAGCAT GCTGGAAGGG TTCGATGAAA ACTTGGATGT ACAGGGGGAG 
TTGATTCTCC AGGATGCCTT TCAAGTGTGG GACCCAAAGT CGCTGATCCG GAAGGGGCGG 
GAGCGCCATC TGTTCCTCTT TGAGATCTCC TTGGTGTTTA GCAAAGAGAT CAAAGACTCT 
TCAGGACACA CGAAATATGT TTACAAGAAC AAGCTACTGA CCTCAGAGCT GGGTGTGACC 
GAGCATGTAG AAGGTGACCC CTGCAAATTC GCCTTGTGGT CTGGGCGCAC CCCATCCTCA 
GACAATAAAA CAGTGCTCAA AGCTTCCAAC ATCGAAACCA AGCAGGAATG GATCAAGAAC 
ATTCGAGAAG TGATTCAGGA GAGGATCATT CATCTTAAAG GCGCTTTAAA GGAGCCAATT 
CAGCTCCCCA AAACACCAGC CAAACTGAGG AACAATAGTA AGAGGGATGG GGTAGAGGAT 
GGTGACAGCC AAGGGGATGG GAGCAGCCAG CCAGATACCA TATCCATTGC CTCCAGGACC 
TCTCAGAACA CAGTGGAAAG TGACAAGCTC TCAGGTGGAT GTGAGCTGAC CGTGGTCCTC 
CAGGACTTCA GCGCAGGCCA CAGCAGCGAG CTGAGCATCC AGGTGGGCCA GACGGTGGAG 
CTGCTGGAAC GACCGAGTGA GAGGCCTGGC TGGTGTCTGG TCCGCACCAC AGAGCGGAGC 
CCCCCACAGG AAGGCCTGGT ACCCAGCAGC GCCCTGTGCA TCTCACACTC TCGCAGCAGC 
GTGGAAATGG ACTGCTTCTT CCCCTTGAAA GATTCATACT CTCATTCCTC CAGTGAGAAT 
GGAGGCAAAT CCGAGTCCGT GGCCCACCTG CAGTCTCAGC CCTCCCTGAA CTCCATCCAC 
AGTTCCCCTG GTCCCAAGCG CTCCACCAAC ACCCTCAAAA AGTGGCTGAC CAGCCCCGTG 
CGCCGCCTCA ACAGCGGAAA AGCCGACGGG AACATCAAAA AGCAGAAGAA AGTCCGGGAT 
GGGCGGAAGA GCTTCGATCT GGGATCGCCC AAGCCTGGGG ATGAGACGAC ACCTCAGGGA 
GACAGCGCTG ACGAGAAGAG CAAGAAGGGT TGGGGCGAAG ACGAGCCAGA TGAAGAGTCA 
CACACACCCC TCCCTCCGCC CATGAAGATC TTTGACAACG ACCCCACACA GGATGAGATG 
TCGTCTTTGC TAGCAGCCCG GCAGGCTCCC CCTGACGTGC CTACTGCTGC AGACCTTGTC 
AGTGCAATAG AAAAGTTGGT CAAAAATAAG CTGACTCTGG AAGGAGGCTC TTACCGGGGG 
AGCTTGAAGG ACCCTACAGG CTGCCTGAAT GAGGGGATGA CCCCTCCCAC ACCTCCTAGA 
AACCTGGAAG AAGAACAGAA AGCCAAGGCG CTGAGAGGCA GGATGTTTGT CCTGAATGAG 
CTGGTACAGA CAGAGAAGGA CTACGTCAAG GACCTGGGGA TTGTGGTGGA GGGCTTCATG 
AAGAGAATAG AAGAGAAGGG GGTCCCTGAG GACATGCGAG GGAAGGAAAA GATCGTGTTT 
GGAAATATCC ACCAGATTTA CGACTGGCAT AAAGATTTTT TCCTGGCTGA ACTGGAAAAA 
TGTATCCAAG AGCAAGACAG ATTGGCCCAG CTCTTCATTA AACACGAGCG GAAGCTGCAC 
ATCTACGTGT GGTACTGCCA GAACAAGCCG CGTTCCGAGT ACATCGTGGC TGAGTACGAT 
GCCTATTTTG AGGAGGTTAA ACAAGAGATC AATCAAAGGC TGACCCTTAG TGACTTCCTG 
ATCAAGCCCA TTCAGAGAAT AACAAAGTAC CAGCTGCTAC TCAAGGACTT CCTGCGATAC 
AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT ATTGAGAAAG CAGTGGAGTT AATGTGCCTT 
GTCCCCAAGC GCTGTAATGA CATGATGAAT CTGGGACGCC TGCAGGGCTT CGAGGGCACC 
CTGACCGCCC AGGGCAAGCT ACTGCAGCAG GACACATTCT ATGTGATCGA GCTGGATGCC 
GGCATGCAGT CCCGGACCAA GGAGAGGCGT GTGTTTCTCT TTGAGCAGAT TGTCATCTTC 
AGTGAACTGC TCCGAAAGGG GTCCCTCACC CCAGGCTACA TGTTCAAAAG AAGCATCAAG 
ATGAATTACT TGGTCCTGGA AGACAATGTG GATGGTGACC CCTGCAAATT TGCACTCATG 
AACAGGGAGA CTTCAGAAAG GGTCATTCTA CAGGCTGCCA ACTCAGACAT CCAGCAGGCC 
TGGGTGCAGG ACATCAACCA AGTCCTAGAA ACACAGAGGG ACTTCTTAAA TGCACTACAG 
TCACCCATTG AGTATCAGCG GAAGGAGAGA AGCACAGCTG TGATCCGGTC CCAGCCCCCG 
AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC 
CCCAAGGGCT CCAGCTATAA CCCGCCTCTG CCACCCCTGA AGATATCTAC CTCTAATGGC 
AGTCCAGGGT TCGACTACCA TCAGCCTGGG GACAAGTTTG ACGCTAGCAA GCAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAGA AGTCATGCTC TTGGCACACT CTACGCATGA GAAAGCGGGC AGACGTGGAG 
AACTCGGGTA AAAATGAAGC CACAGGGCCT CGTAAACCCA AGGATATTCT GGGCAACAAA 
GTCTCTGTTA AAGAGACGAA CAGTTCCGAG GAGTCAGAGT GTGATGATCT TGACCCTAAT 
ACTAGTATGG AGATCTTGAA TCCAAATTTC ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG 
CCCTTAGTAG ATGTGACCTG TTTGCTTGGA GACACAGTGC TACTGCAGTG TAAAGCCTGT 
GGGCGGCCAA AGCCCAGCAT CACCTGGAAG GGTCCGGATC AGAACATCCT AGACACTGAC 
AACAGTTCAG CAACATACAC CATCTCCTCC TGTGATTCTG GGGAAAGCAC CCTCAAGATC 
TGCAACCTGA TGCCCCAGGA TAGTGGGATT TATACCTGCA TAGCAGCAAA CGACCACGGG 
ACTGCATCCA CATCTGCTAC AGTTAAGGTG CAAGGTGTCC CCGCAGCCCC CAACCGCCCA 
ATCGCACAGG AGAGGAGCTG CACCTCCGTC ATCCTCCGCT GGCTGCCCCC AGCCAGCACC 
GGAAACTGCA CTATTTCGGG GTACACAGTG GAGTATCGCG AGGAAGGTTC CCAGGTCTGG 
CAGCAATCCG TAGCTTCGAC CTTGGACACT TACCTGGTCA TTGAAGACCT TAGTCCTGGG 
TGCCCTTATC AGTTCAGGGT CAGTGCCAGC AACCCCTGGG GAATCAGCCT TCCCAGTGAG 
CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC GATGCAGCCG CCGACGGTGC CACCATTTCT 
TGGAAGGAAA ATTTTGATTC TGCTTACACT GAACTGAACG AGATTGGCAG AGGCCGTTTC 
TCTATTGTAA AGAAATGCAT TCACAAAGCT ACCCGCAAAG ATGTTGCTGT GAAATTTGTG 
AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT GCTCACGAGG CCGCCCTGCT CCAGCACCTG 
CAGCACCCTC AGTATGTCAC GCTCCATGAC ACCTACGAGT CCCCCACATC CTACATCCTG 
ATTTTGGAAC TGATGGATGA CGGCCGACTC TTGGACTACC TTATGAATCA TGATGAGCTG 
ATGGAAGAAA AGGTAGCTTT CTACATTCGA GACATCATGG AAGCTCTGCA GTACCTGCAC 
AACTGTAGGG TCGCACATTT GGACATAAAG CCCGAAAACC TGCTCATTGA CCTCCGGATC 
CCAGTGCCTC GAGTGAAGCT CATCGACTTG GAAGACGCTG TCCAGATCTC AGGCCACTTC 
CATATCCACC ATCTGCTGGG GAACCCTGAG TTCGCTGCCC CCGAAGTGAT CCAAGGCATC 
CCTGTCTCCC TGGGCACAGA CATCTGGAGC ATCGGGGTTC TGACATATGT CATGCTTAGT 
GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA GAGGAAACCT GTATCAACGT GTGCAGGGTG 
GACTTTAGCT TCCCCCACGA GTACTTCTGC GGTGTGAGCA ATGCTGCCAG GGATTTCATC 
AACGTAATCT TACAGGAAGA CTTCCGGAGG CGGCCCACAG CAGCCACGTG CCTGCAGCAT 
CCCTGGCTAC AGCCCCACAA CGGCAGCTAC TCCAAGATCC CCTTGGACAC CTCCCGTCTG 
GCCTGCTTCA TAGAACGCCG CAAGCACCAG AATGATGTGC GGCCCATTCC CAATGTCAAG 
AGTTACATCG TCAATAGGGT GAACCAAGGG ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522433.1
CDS	24875..33808
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_006522370.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522370.4

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGAA GACGCACCAG 
AGCGCCCTGC AGGTACAGCA GAAGGCAGAG GCACTGCTCC AGGCCGGCCA CTACGACGCA 
GACGCCATCC GGGAATGTGC TGAGAAGGTG GCCCTCCACT GGCAACAGCT CATGCTAAAG 
ATGGAGGACC GGCTGAAGCT TGTCAACGCC TCTGTGGCCT TTTACAAGAC CTCTGAGCAG 
GTGTGCAGTG TTCTGGAGAG CTTGGAGCAG GAGTACCGGA GAGACGAGGA CTGGTGTGGT 
GGGAGAGACA AGCTGGGGCC GGCGGCAGAG ATGGACCACG TTATTCCACT CCTCAGCAAG 
CACCTGGAGC AGAAGGAGGC CTTCCTGAAG GCCTGCACGC TGGCAAGGCG GAACGCCGAG 
GTGTTCCTCA AATACATCCA CAGGAACAAT GTGAGCATGC CTAGTGTTGC CAGCCACACC 
CGGGGGCCTG AACAGCAAGT AAAAGCCATC TTGAGCGAGC TCTTGCAGAG GGAGAATCGA 
GTCCTGCACT TCTGGACCTT GAAGAAGCGG CGGTTAGACC AGTGTCAGCA ATATGTGGTG 
TTTGAGCGCA GTGCTAAGCA GGCACTGGAC TGGATCCAAG AAACAGGAGA ATATTACCTC 
TCAACACATA CCTCCACCGG AGAGACCACG GAGGAGACTC AGGAGCTGCT GAAAGAGTAT 
GGAGAATTCA GGGTGCCTGC CAAGCAAACG AAGGAGAAGG TGAAACTTCT GATCCAGCTA 
GCAGACAGCT TTGTGGAAAA GGGCCACATC CACGCCACAG AGATACGGAA ATGGGTGACC 
ACGGTGGACA AGCACTACCG CGATTTCTCC CTGAGGATGG GGAAGTACCG GTACTCGCTG 
GAGAAGGCGC TGGGGGTCAA CACGGAGGAT AACAAGGACC TAGAGCTGGA CATCATCCCA 
GCAAGCCTTT CGGATCGGGA GGTCAAGCTC CGGGACGCTA ACCATGAGAT CAATGAAGAG 
AAACGGAAGT CAGCTCGAAA GAAAGAGTTC ATTATGGCTG AACTGCTCCA AACTGAGAAG 
GCTTATGTAA GGGACTTGCA CGAGTGCTTG GAGACCTACC TGTGGGAGAT GACTAGTGGT 
GTGGAAGAGA TACCCCCCGG CATCCTCAAT AAGGAACATA TCATCTTCGG CAACATCCAG 
GAGATCTATG ACTTCCATAA CAACATCTTC CTCAAAGAGC TGGAGAAGTA CGAGCAGCTG 
CCTGAGGATG TGGGACACTG CTTTGTTACC TGGGCAGACA AATTTCAGAT GTATGTCACC 
TACTGTAAAA ACAAGCCTGA TTCCAACCAG CTGATCCTGG AGCATGCGGG CACCTTCTTT 
GATGAGATCC AGCAGCGGCA TGGCCTGGCC AACTCCATCT CCTCCTACCT AATTAAGCCT 
GTCCAGAGGG TTACCAAGTA CCAGCTGCTC CTGAAGGAAC TTCTAACTTG CTGTGAAGAA 
GGGAAAGGAG AGCTGAAGGA TGGCCTGGAG GTGATGCTCA GTGTCCCCAA GAAAGCCAAC 
GATGCCATGC ATGTCAGCAT GCTGGAAGGG TTCGATGAAA ACTTGGATGT ACAGGGGGAG 
TTGATTCTCC AGGATGCCTT TCAAGTGTGG GACCCAAAGT CGCTGATCCG GAAGGGGCGG 
GAGCGCCATC TGTTCCTCTT TGAGATCTCC TTGGTGTTTA GCAAAGAGAT CAAAGACTCT 
TCAGGACACA CGAAATATGT TTACAAGAAC AAGCTACTGA CCTCAGAGCT GGGTGTGACC 
GAGCATGTAG AAGGTGACCC CTGCAAATTC GCCTTGTGGT CTGGGCGCAC CCCATCCTCA 
GACAATAAAA CAGTGCTCAA AGCTTCCAAC ATCGAAACCA AGCAGGAATG GATCAAGAAC 
ATTCGAGAAG TGATTCAGGA GAGGATCATT CATCTTAAAG GCGCTTTAAA GGAGCCAATT 
CAGCTCCCCA AAACACCAGC CAAACTGAGG AACAATAGTA AGAGGGATGG GGTAGAGGAT 
GGTGACAGCC AAGGGGATGG GAGCAGCCAG CCAGATACCA TATCCATTGC CTCCAGGACC 
TCTCAGAACA CAGTGGAAAG TGACAAGCTC TCAGGTGGAT GTGAGCTGAC CGTGGTCCTC 
CAGGACTTCA GCGCAGGCCA CAGCAGCGAG CTGAGCATCC AGGTGGGCCA GACGGTGGAG 
CTGCTGGAAC GACCGAGTGA GAGGCCTGGC TGGTGTCTGG TCCGCACCAC AGAGCGGAGC 
CCCCCACAGG AAGGCCTGGT ACCCAGCAGC GCCCTGTGCA TCTCACACTC TCGCAGCAGC 
GTGGAAATGG ACTGCTTCTT CCCCTTGAAA GATTCATACT CTCATTCCTC CAGTGAGAAT 
GGAGGCAAAT CCGAGTCCGT GGCCCACCTG CAGTCTCAGC CCTCCCTGAA CTCCATCCAC 
AGTTCCCCTG GTCCCAAGCG CTCCACCAAC ACCCTCAAAA AGTGGCTGAC CAGCCCCGTG 
CGCCGCCTCA ACAGCGGAAA AGCCGACGGG AACATCAAAA AGCAGAAGAA AGTCCGGGAT 
GGGCGGAAGA GCTTCGATCT GGGATCGCCC AAGCCTGGGG ATGAGACGAC ACCTCAGGGA 
GACAGCGCTG ACGAGAAGAG CAAGAAGGGT TGGGGCGAAG ACGAGCCAGA TGAAGAGTCA 
CACACACCCC TCCCTCCGCC CATGAAGATC TTTGACAACG ACCCCACACA GGATGAGATG 
TCGTCTTTGC TAGCAGCCCG GCAGGCTCCC CCTGACGTGC CTACTGCTGC AGACCTTGTC 
AGTGCAATAG AAAAGTTGGT CAAAAATAAG CTGACTCTGG AAGGAGGCTC TTACCGGGGG 
AGCTTGAAGG ACCCTACAGG CTGCCTGAAT GAGGGGATGA CCCCTCCCAC ACCTCCTAGA 
AACCTGGAAG AAGAACAGAA AGCCAAGGCG CTGAGAGGCA GGATGTTTGT CCTGAATGAG 
CTGGTACAGA CAGAGAAGGA CTACGTCAAG GACCTGGGGA TTGTGGTGGA GGGCTTCATG 
AAGAGAATAG AAGAGAAGGG GGTCCCTGAG GACATGCGAG GGAAGGAAAA GATCGTGTTT 
GGAAATATCC ACCAGATTTA CGACTGGCAT AAAGATTTTT TCCTGGCTGA ACTGGAAAAA 
TGTATCCAAG AGCAAGACAG ATTGGCCCAG CTCTTCATTA AACACGAGCG GAAGCTGCAC 
ATCTACGTGT GGTACTGCCA GAACAAGCCG CGTTCCGAGT ACATCGTGGC TGAGTACGAT 
GCCTATTTTG AGGAGGTTAA ACAAGAGATC AATCAAAGGC TGACCCTTAG TGACTTCCTG 
ATCAAGCCCA TTCAGAGAAT AACAAAGTAC CAGCTGCTAC TCAAGGACTT CCTGCGATAC 
AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT ATTGAGAAAG CAGTGGAGTT AATGTGCCTT 
GTCCCCAAGC GCTGTAATGA CATGATGAAT CTGGGACGCC TGCAGGGCTT CGAGGGCACC 
CTGACCGCCC AGGGCAAGCT ACTGCAGCAG GACACATTCT ATGTGATCGA GCTGGATGCC 
GGCATGCAGT CCCGGACCAA GGAGAGGCGT GTGTTTCTCT TTGAGCAGAT TGTCATCTTC 
AGTGAACTGC TCCGAAAGGG GTCCCTCACC CCAGGCTACA TGTTCAAAAG AAGCATCAAG 
ATGAATTACT TGGTCCTGGA AGACAATGTG GATGGTGACC CCTGCAAATT TGCACTCATG 
AACAGGGAGA CTTCAGAAAG GGTCATTCTA CAGGCTGCCA ACTCAGACAT CCAGCAGGCC 
TGGGTGCAGG ACATCAACCA AGTCCTAGAA ACACAGAGGG ACTTCTTAAA TGCACTACAG 
TCACCCATTG AGTATCAGCG GAAGGAGAGA AGCACAGCTG TGATCCGGTC CCAGCCCCCG 
AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC 
CCCAAGGGCT CCAGCTATAA CCCGCCTCTG CCACCCCTGA AGATATCTAC CTCTAATGGC 
AGTCCAGGGT TCGACTACCA TCAGCCTGGG GACAAGTTTG ACGCTAGCAA GCAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAGA AGTCATGCTC TTGGCACACT CTACGCATGA GAAAGCGGGC AGACGTGGAG 
AACTCGGGTA AAAATGAAGC CACAGGGCCT CGTAAACCCA AGGATATTCT GGGCAACAAA 
GTCTCTGTTA AAGAGACGAA CAGTTCCGAG GAGTCAGAGT GTGATGATCT TGACCCTAAT 
ACTAGTATGG AGATCTTGAA TCCAAATTTC ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG 
CCCTTAGTAG ATGTGACCTG TTTGCTTGGA GACACAGTGC TACTGCAGTG TAAAGCCTGT 
GGGCGGCCAA AGCCCAGCAT CACCTGGAAG GGTCCGGATC AGAACATCCT AGACACTGAC 
AACAGTTCAG CAACATACAC CATCTCCTCC TGTGATTCTG GGGAAAGCAC CCTCAAGATC 
TGCAACCTGA TGCCCCAGGA TAGTGGGATT TATACCTGCA TAGCAGCAAA CGACCACGGG 
ACTGCATCCA CATCTGCTAC AGTTAAGGTG CAAGGTGTCC CCGCAGCCCC CAACCGCCCA 
ATCGCACAGG AGAGGAGCTG CACCTCCGTC ATCCTCCGCT GGCTGCCCCC AGCCAGCACC 
GGAAACTGCA CTATTTCGGG GTACACAGTG GAGTATCGCG AGGAAGGTTC CCAGGTCTGG 
CAGCAATCCG TAGCTTCGAC CTTGGACACT TACCTGGTCA TTGAAGACCT TAGTCCTGGG 
TGCCCTTATC AGTTCAGGGT CAGTGCCAGC AACCCCTGGG GAATCAGCCT TCCCAGTGAG 
CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC GATGCAGCCG CCGACGGTGC CACCATTTCT 
TGGAAGGAAA ATTTTGATTC TGCTTACACT GAACTGAACG AGATTGGCAG AGGCCGTTTC 
TCTATTGTAA AGAAATGCAT TCACAAAGCT ACCCGCAAAG ATGTTGCTGT GAAATTTGTG 
AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT GCTCACGAGG CCGCCCTGCT CCAGCACCTG 
CAGCACCCTC AGTATGTCAC GCTCCATGAC ACCTACGAGT CCCCCACATC CTACATCCTG 
ATTTTGGAAC TGATGGATGA CGGCCGACTC TTGGACTACC TTATGAATCA TGATGAGCTG 
ATGGAAGAAA AGGTAGCTTT CTACATTCGA GACATCATGG AAGCTCTGCA GTACCTGCAC 
AACTGTAGGG TCGCACATTT GGACATAAAG CCCGAAAACC TGCTCATTGA CCTCCGGATC 
CCAGTGCCTC GAGTGAAGCT CATCGACTTG GAAGACGCTG TCCAGATCTC AGGCCACTTC 
CATATCCACC ATCTGCTGGG GAACCCTGAG TTCGCTGCCC CCGAAGTGAT CCAAGGCATC 
CCTGTCTCCC TGGGCACAGA CATCTGGAGC ATCGGGGTTC TGACATATGT CATGCTTAGT 
GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA GAGGAAACCT GTATCAACGT GTGCAGGGTG 
GACTTTAGCT TCCCCCACGA GTACTTCTGC GGTGTGAGCA ATGCTGCCAG GGATTTCATC 
AACGTAATCT TACAGGAAGA CTTCCGGAGG CGGCCCACAG CAGCCACGTG CCTGCAGCAT 
CCCTGGCTAC AGCCCCACAA CGGCAGCTAC TCCAAGATCC CCTTGGACAC CTCCCGTCTG 
GCCTGCTTCA TAGAACGCCG CAAGCACCAG AATGATGTGC GGCCCATTCC CAATGTCAAG 
AGTTACATCG TCAATAGGGT GAACCAAGGG ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522386.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522449.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X41, mRNA.

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CloneID	OMu52302
Clone ID Related Accession (Same CDS sequence)	XM_006522386.3 , XM_006522386.4
Accession Version	XM_006522386.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X38
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522386.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGAA GACGCACCAG 
AGCGCCCTGC AGGTACAGCA GAAGGCAGAG GCACTGCTCC AGGCCGGCCA CTACGACGCA 
GACGCCATCC GGGAATGTGC TGAGAAGGTG GCCCTCCACT GGCAACAGCT CATGCTAAAG 
ATGGAGGACC GGCTGAAGCT TGTCAACGCC TCTGTGGCCT TTTACAAGAC CTCTGAGCAG 
GTGTGCAGTG TTCTGGAGAG CTTGGAGCAG GAGTACCGGA GAGACGAGGA CTGGTGTGGT 
GGGAGAGACA AGCTGGGGCC GGCGGCAGAG ATGGACCACG TTATTCCACT CCTCAGCAAG 
CACCTGGAGC AGAAGGAGGC CTTCCTGAAG GCCTGCACGC TGGCAAGGCG GAACGCCGAG 
GTGTTCCTCA AATACATCCA CAGGAACAAT GTGAGCATGC CTAGTGTTGC CAGCCACACC 
CGGGGGCCTG AACAGCAAGT AAAAGCCATC TTGAGCGAGC TCTTGCAGAG GGAGAATCGA 
GTCCTGCACT TCTGGACCTT GAAGAAGCGG CGGTTAGACC AGTGTCAGCA ATATGTGGTG 
TTTGAGCGCA GTGCTAAGCA GGCACTGGAC TGGATCCAAG AAACAGGAGA ATATTACCTC 
TCAACACATA CCTCCACCGG AGAGACCACG GAGGAGACTC AGGAGCTGCT GAAAGAGTAT 
GGAGAATTCA GGGTGCCTGC CAAGCAAACG AAGGAGAAGG TGAAACTTCT GATCCAGCTA 
GCAGACAGCT TTGTGGAAAA GGGCCACATC CACGCCACAG AGATACGGAA ATGGGTGACC 
ACGGTGGACA AGCACTACCG CGATTTCTCC CTGAGGATGG GGAAGTACCG GTACTCGCTG 
GAGAAGGCGC TGGGGGTCAA CACGGAGGAT AACAAGGACC TAGAGCTGGA CATCATCCCA 
GCAAGCCTTT CGGATCGGGA GGTCAAGCTC CGGGACGCTA ACCATGAGAT CAATGAAGAG 
AAACGGAAGT CAGCTCGAAA GAAAGAGTTC ATTATGGCTG AACTGCTCCA AACTGAGAAG 
GCTTATGTAA GGGACTTGCA CGAGTGCTTG GAGACCTACC TGTGGGAGAT GACTAGTGGT 
GTGGAAGAGA TACCCCCCGG CATCCTCAAT AAGGAACATA TCATCTTCGG CAACATCCAG 
GAGATCTATG ACTTCCATAA CAACATCTTC CTCAAAGAGC TGGAGAAGTA CGAGCAGCTG 
CCTGAGGATG TGGGACACTG CTTTGTTACC TGGGCAGACA AATTTCAGAT GTATGTCACC 
TACTGTAAAA ACAAGCCTGA TTCCAACCAG CTGATCCTGG AGCATGCGGG CACCTTCTTT 
GATGAGATCC AGCAGCGGCA TGGCCTGGCC AACTCCATCT CCTCCTACCT AATTAAGCCT 
GTCCAGAGGG TTACCAAGTA CCAGCTGCTC CTGAAGGAAC TTCTAACTTG CTGTGAAGAA 
GGGAAAGGAG AGCTGAAGGA TGGCCTGGAG GTGATGCTCA GTGTCCCCAA GAAAGCCAAC 
GATGCCATGC ATGTCAGCAT GCTGGAAGGG TTCGATGAAA ACTTGGATGT ACAGGGGGAG 
TTGATTCTCC AGGATGCCTT TCAAGTGTGG GACCCAAAGT CGCTGATCCG GAAGGGGCGG 
GAGCGCCATC TGTTCCTCTT TGAGATCTCC TTGGTGTTTA GCAAAGAGAT CAAAGACTCT 
TCAGGACACA CGAAATATGT TTACAAGAAC AAGCTACTGA CCTCAGAGCT GGGTGTGACC 
GAGCATGTAG AAGGTGACCC CTGCAAATTC GCCTTGTGGT CTGGGCGCAC CCCATCCTCA 
GACAATAAAA CAGTGCTCAA AGCTTCCAAC ATCGAAACCA AGCAGGAATG GATCAAGAAC 
ATTCGAGAAG TGATTCAGGA GAGGATCATT CATCTTAAAG GCGCTTTAAA GGAGCCAATT 
CAGCTCCCCA AAACACCAGC CAAACTGAGG AACAATAGTA AGAGGGATGG GGTAGAGGAT 
GGTGACAGCC AAGGGGATGG GAGCAGCCAG CCAGATACCA TATCCATTGC CTCCAGGACC 
TCTCAGAACA CAGTGGAAAG TGACAAGCTC TCAGGTGGAT GTGAGCTGAC CGTGGTCCTC 
CAGGACTTCA GCGCAGGCCA CAGCAGCGAG CTGAGCATCC AGGTGGGCCA GACGGTGGAG 
CTGCTGGAAC GACCGAGTGA GAGGCCTGGC TGGTGTCTGG TCCGCACCAC AGAGCGGAGC 
CCCCCACAGG AAGGCCTGGT ACCCAGCAGC GCCCTGTGCA TCTCACACTC TCGCAGCAGC 
GTGGAAATGG ACTGCTTCTT CCCCTTGAAA GATTCATACT CTCATTCCTC CAGTGAGAAT 
GGAGGCAAAT CCGAGTCCGT GGCCCACCTG CAGTCTCAGC CCTCCCTGAA CTCCATCCAC 
AGTTCCCCTG GTCCCAAGCG CTCCACCAAC ACCCTCAAAA AGTGGCTGAC CAGCCCCGTG 
CGCCGCCTCA ACAGCGGAAA AGCCGACGGG AACATCAAAA AGCAGAAGAA AGTCCGGGAT 
GGGCGGAAGA GCTTCGATCT GGGATCGCCC AAGCCTGGGG ATGAGACGAC ACCTCAGGGA 
GACAGCGCTG ACGAGAAGAG CAAGAAGGGT TGGGGCGAAG ACGAGCCAGA TGAAGAGTCA 
CACACACCCC TCCCTCCGCC CATGAAGATC TTTGACAACG ACCCCACACA GGATGAGATG 
TCGTCTTTGC TAGCAGCCCG GCAGGCTCCC CCTGACGTGC CTACTGCTGC AGACCTTGTC 
AGTGCAATAG AAAAGTTGGT CAAAAATAAG CTGACTCTGG AAGGAGGCTC TTACCGGGGG 
AGCTTGAAGG ACCCTACAGG CTGCCTGAAT GAGGGGATGA CCCCTCCCAC ACCTCCTAGA 
AACCTGGAAG AAGAACAGAA AGCCAAGGCG CTGAGAGGCA GGATGTTTGT CCTGAATGAG 
CTGGTACAGA CAGAGAAGGA CTACGTCAAG GACCTGGGGA TTGTGGTGGA GGGCTTCATG 
AAGAGAATAG AAGAGAAGGG GGTCCCTGAG GACATGCGAG GGAAGGAAAA GATCGTGTTT 
GGAAATATCC ACCAGATTTA CGACTGGCAT AAAGATTTTT TCCTGGCTGA ACTGGAAAAA 
TGTATCCAAG AGCAAGACAG ATTGGCCCAG CTCTTCATTA AACACGAGCG GAAGCTGCAC 
ATCTACGTGT GGTACTGCCA GAACAAGCCG CGTTCCGAGT ACATCGTGGC TGAGTACGAT 
GCCTATTTTG AGGAGGTTAA ACAAGAGATC AATCAAAGGC TGACCCTTAG TGACTTCCTG 
ATCAAGCCCA TTCAGAGAAT AACAAAGTAC CAGCTGCTAC TCAAGGACTT CCTGCGATAC 
AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT ATTGAGAAAG CAGTGGAGTT AATGTGCCTT 
GTCCCCAAGC GCTGTAATGA CATGATGAAT CTGGGACGCC TGCAGGGCTT CGAGGGCACC 
CTGACCGCCC AGGGCAAGCT ACTGCAGCAG GACACATTCT ATGTGATCGA GCTGGATGCC 
GGCATGCAGT CCCGGACCAA GGAGAGGCGT GTGTTTCTCT TTGAGCAGAT TGTCATCTTC 
AGTGAACTGC TCCGAAAGGG GTCCCTCACC CCAGGCTACA TGTTCAAAAG AAGCATCAAG 
ATGAATTACT TGGTCCTGGA AGACAATGTG GATGGTGACC CCTGCAAATT TGCACTCATG 
AACAGGGAGA CTTCAGAAAG GGTCATTCTA CAGGCTGCCA ACTCAGACAT CCAGCAGGCC 
TGGGTGCAGG ACATCAACCA AGTCCTAGAA ACACAGAGGG ACTTCTTAAA TGCACTACAG 
TCACCCATTG AGTATCAGCG GAAGGAGAGA AGCACAGCTG TGATCCGGTC CCAGCCCCCG 
AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC 
CCCAAGGGCT CCAGCTATAA CCCGCCTCTG CCACCCCTGA AGATATCTAC CTCTAATGGC 
AGTCCAGGGT TCGACTACCA TCAGCCTGGG GACAAGTTTG ACGCTAGCAA GCAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAGA AGTCATGCTC TTGGCACACT CTACGCATGA GAAAGCGGGC AGACGTGGAG 
AACTCGGGTA AAAATGAAGC CACAGGGCCT CGTAAACCCA AGGATATTCT GGGCAACAAA 
GTCTCTGTTA AAATCTTGAA TCCAAATTTC ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG 
CCCTTAGTAG ATGTGACCTG TTTGCTTGGA GACACAGTGC TACTGCAGTG TAAAGCCTGT 
GGGCGGCCAA AGCCCAGCAT CACCTGGAAG GGTCCGGATC AGAACATCCT AGACACTGAC 
AACAGTTCAG CAACATACAC CATCTCCTCC TGTGATTCTG GGGAAAGCAC CCTCAAGATC 
TGCAACCTGA TGCCCCAGGA TAGTGGGATT TATACCTGCA TAGCAGCAAA CGACCACGGG 
ACTGCATCCA CATCTGCTAC AGTTAAGGTG CAAGGTGTCC CCGCAGCCCC CAACCGCCCA 
ATCGCACAGG AGAGGAGCTG CACCTCCGTC ATCCTCCGCT GGCTGCCCCC AGCCAGCACC 
GGAAACTGCA CTATTTCGGG GTACACAGTG GAGTATCGCG AGGAAGGTTC CCAGGTCTGG 
CAGCAATCCG TAGCTTCGAC CTTGGACACT TACCTGGTCA TTGAAGACCT TAGTCCTGGG 
TGCCCTTATC AGTTCAGGGT CAGTGCCAGC AACCCCTGGG GAATCAGCCT TCCCAGTGAG 
CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC GATGCAGCCG CCGACGGTGC CACCATTTCT 
TGGAAGGAAA ATTTTGATTC TGCTTACACT GAACTGAACG AGATTGGCAG AGGCCGTTTC 
TCTATTGTAA AGAAATGCAT TCACAAAGCT ACCCGCAAAG ATGTTGCTGT GAAATTTGTG 
AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT GCTCACGAGG CCGCCCTGCT CCAGCACCTG 
CAGCACCCTC AGTATGTCAC GCTCCATGAC ACCTACGAGT CCCCCACATC CTACATCCTG 
ATTTTGGAAC TGATGGATGA CGGCCGACTC TTGGACTACC TTATGAATCA TGATGAGCTG 
ATGGAAGAAA AGGTAGCTTT CTACATTCGA GACATCATGG AAGCTCTGCA GTACCTGCAC 
AACTGTAGGG TCGCACATTT GGACATAAAG CCCGAAAACC TGCTCATTGA CCTCCGGATC 
CCAGTGCCTC GAGTGAAGCT CATCGACTTG GAAGACGCTG TCCAGATCTC AGGCCACTTC 
CATATCCACC ATCTGCTGGG GAACCCTGAG TTCGCTGCCC CCGAAGTGAT CCAAGGCATC 
CCTGTCTCCC TGGGCACAGA CATCTGGAGC ATCGGGGTTC TGACATATGT CATGCTTAGT 
GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA GAGGAAACCT GTATCAACGT GTGCAGGGTG 
GACTTTAGCT TCCCCCACGA GTACTTCTGC GGTGTGAGCA ATGCTGCCAG GGATTTCATC 
AACGTAATCT TACAGGAAGA CTTCCGGAGG CGGCCCACAG CAGCCACGTG CCTGCAGCAT 
CCCTGGCTAC AGCCCCACAA CGGCAGCTAC TCCAAGATCC CCTTGGACAC CTCCCGTCTG 
GCCTGCTTCA TAGAACGCCG CAAGCACCAG AATGATGTGC GGCCCATTCC CAATGTCAAG 
AGTTACATCG TCAATAGGGT GAACCAAGGG ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522449.1
CDS	4891..13764
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_006522386.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522386.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGAA GACGCACCAG 
AGCGCCCTGC AGGTACAGCA GAAGGCAGAG GCACTGCTCC AGGCCGGCCA CTACGACGCA 
GACGCCATCC GGGAATGTGC TGAGAAGGTG GCCCTCCACT GGCAACAGCT CATGCTAAAG 
ATGGAGGACC GGCTGAAGCT TGTCAACGCC TCTGTGGCCT TTTACAAGAC CTCTGAGCAG 
GTGTGCAGTG TTCTGGAGAG CTTGGAGCAG GAGTACCGGA GAGACGAGGA CTGGTGTGGT 
GGGAGAGACA AGCTGGGGCC GGCGGCAGAG ATGGACCACG TTATTCCACT CCTCAGCAAG 
CACCTGGAGC AGAAGGAGGC CTTCCTGAAG GCCTGCACGC TGGCAAGGCG GAACGCCGAG 
GTGTTCCTCA AATACATCCA CAGGAACAAT GTGAGCATGC CTAGTGTTGC CAGCCACACC 
CGGGGGCCTG AACAGCAAGT AAAAGCCATC TTGAGCGAGC TCTTGCAGAG GGAGAATCGA 
GTCCTGCACT TCTGGACCTT GAAGAAGCGG CGGTTAGACC AGTGTCAGCA ATATGTGGTG 
TTTGAGCGCA GTGCTAAGCA GGCACTGGAC TGGATCCAAG AAACAGGAGA ATATTACCTC 
TCAACACATA CCTCCACCGG AGAGACCACG GAGGAGACTC AGGAGCTGCT GAAAGAGTAT 
GGAGAATTCA GGGTGCCTGC CAAGCAAACG AAGGAGAAGG TGAAACTTCT GATCCAGCTA 
GCAGACAGCT TTGTGGAAAA GGGCCACATC CACGCCACAG AGATACGGAA ATGGGTGACC 
ACGGTGGACA AGCACTACCG CGATTTCTCC CTGAGGATGG GGAAGTACCG GTACTCGCTG 
GAGAAGGCGC TGGGGGTCAA CACGGAGGAT AACAAGGACC TAGAGCTGGA CATCATCCCA 
GCAAGCCTTT CGGATCGGGA GGTCAAGCTC CGGGACGCTA ACCATGAGAT CAATGAAGAG 
AAACGGAAGT CAGCTCGAAA GAAAGAGTTC ATTATGGCTG AACTGCTCCA AACTGAGAAG 
GCTTATGTAA GGGACTTGCA CGAGTGCTTG GAGACCTACC TGTGGGAGAT GACTAGTGGT 
GTGGAAGAGA TACCCCCCGG CATCCTCAAT AAGGAACATA TCATCTTCGG CAACATCCAG 
GAGATCTATG ACTTCCATAA CAACATCTTC CTCAAAGAGC TGGAGAAGTA CGAGCAGCTG 
CCTGAGGATG TGGGACACTG CTTTGTTACC TGGGCAGACA AATTTCAGAT GTATGTCACC 
TACTGTAAAA ACAAGCCTGA TTCCAACCAG CTGATCCTGG AGCATGCGGG CACCTTCTTT 
GATGAGATCC AGCAGCGGCA TGGCCTGGCC AACTCCATCT CCTCCTACCT AATTAAGCCT 
GTCCAGAGGG TTACCAAGTA CCAGCTGCTC CTGAAGGAAC TTCTAACTTG CTGTGAAGAA 
GGGAAAGGAG AGCTGAAGGA TGGCCTGGAG GTGATGCTCA GTGTCCCCAA GAAAGCCAAC 
GATGCCATGC ATGTCAGCAT GCTGGAAGGG TTCGATGAAA ACTTGGATGT ACAGGGGGAG 
TTGATTCTCC AGGATGCCTT TCAAGTGTGG GACCCAAAGT CGCTGATCCG GAAGGGGCGG 
GAGCGCCATC TGTTCCTCTT TGAGATCTCC TTGGTGTTTA GCAAAGAGAT CAAAGACTCT 
TCAGGACACA CGAAATATGT TTACAAGAAC AAGCTACTGA CCTCAGAGCT GGGTGTGACC 
GAGCATGTAG AAGGTGACCC CTGCAAATTC GCCTTGTGGT CTGGGCGCAC CCCATCCTCA 
GACAATAAAA CAGTGCTCAA AGCTTCCAAC ATCGAAACCA AGCAGGAATG GATCAAGAAC 
ATTCGAGAAG TGATTCAGGA GAGGATCATT CATCTTAAAG GCGCTTTAAA GGAGCCAATT 
CAGCTCCCCA AAACACCAGC CAAACTGAGG AACAATAGTA AGAGGGATGG GGTAGAGGAT 
GGTGACAGCC AAGGGGATGG GAGCAGCCAG CCAGATACCA TATCCATTGC CTCCAGGACC 
TCTCAGAACA CAGTGGAAAG TGACAAGCTC TCAGGTGGAT GTGAGCTGAC CGTGGTCCTC 
CAGGACTTCA GCGCAGGCCA CAGCAGCGAG CTGAGCATCC AGGTGGGCCA GACGGTGGAG 
CTGCTGGAAC GACCGAGTGA GAGGCCTGGC TGGTGTCTGG TCCGCACCAC AGAGCGGAGC 
CCCCCACAGG AAGGCCTGGT ACCCAGCAGC GCCCTGTGCA TCTCACACTC TCGCAGCAGC 
GTGGAAATGG ACTGCTTCTT CCCCTTGAAA GATTCATACT CTCATTCCTC CAGTGAGAAT 
GGAGGCAAAT CCGAGTCCGT GGCCCACCTG CAGTCTCAGC CCTCCCTGAA CTCCATCCAC 
AGTTCCCCTG GTCCCAAGCG CTCCACCAAC ACCCTCAAAA AGTGGCTGAC CAGCCCCGTG 
CGCCGCCTCA ACAGCGGAAA AGCCGACGGG AACATCAAAA AGCAGAAGAA AGTCCGGGAT 
GGGCGGAAGA GCTTCGATCT GGGATCGCCC AAGCCTGGGG ATGAGACGAC ACCTCAGGGA 
GACAGCGCTG ACGAGAAGAG CAAGAAGGGT TGGGGCGAAG ACGAGCCAGA TGAAGAGTCA 
CACACACCCC TCCCTCCGCC CATGAAGATC TTTGACAACG ACCCCACACA GGATGAGATG 
TCGTCTTTGC TAGCAGCCCG GCAGGCTCCC CCTGACGTGC CTACTGCTGC AGACCTTGTC 
AGTGCAATAG AAAAGTTGGT CAAAAATAAG CTGACTCTGG AAGGAGGCTC TTACCGGGGG 
AGCTTGAAGG ACCCTACAGG CTGCCTGAAT GAGGGGATGA CCCCTCCCAC ACCTCCTAGA 
AACCTGGAAG AAGAACAGAA AGCCAAGGCG CTGAGAGGCA GGATGTTTGT CCTGAATGAG 
CTGGTACAGA CAGAGAAGGA CTACGTCAAG GACCTGGGGA TTGTGGTGGA GGGCTTCATG 
AAGAGAATAG AAGAGAAGGG GGTCCCTGAG GACATGCGAG GGAAGGAAAA GATCGTGTTT 
GGAAATATCC ACCAGATTTA CGACTGGCAT AAAGATTTTT TCCTGGCTGA ACTGGAAAAA 
TGTATCCAAG AGCAAGACAG ATTGGCCCAG CTCTTCATTA AACACGAGCG GAAGCTGCAC 
ATCTACGTGT GGTACTGCCA GAACAAGCCG CGTTCCGAGT ACATCGTGGC TGAGTACGAT 
GCCTATTTTG AGGAGGTTAA ACAAGAGATC AATCAAAGGC TGACCCTTAG TGACTTCCTG 
ATCAAGCCCA TTCAGAGAAT AACAAAGTAC CAGCTGCTAC TCAAGGACTT CCTGCGATAC 
AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT ATTGAGAAAG CAGTGGAGTT AATGTGCCTT 
GTCCCCAAGC GCTGTAATGA CATGATGAAT CTGGGACGCC TGCAGGGCTT CGAGGGCACC 
CTGACCGCCC AGGGCAAGCT ACTGCAGCAG GACACATTCT ATGTGATCGA GCTGGATGCC 
GGCATGCAGT CCCGGACCAA GGAGAGGCGT GTGTTTCTCT TTGAGCAGAT TGTCATCTTC 
AGTGAACTGC TCCGAAAGGG GTCCCTCACC CCAGGCTACA TGTTCAAAAG AAGCATCAAG 
ATGAATTACT TGGTCCTGGA AGACAATGTG GATGGTGACC CCTGCAAATT TGCACTCATG 
AACAGGGAGA CTTCAGAAAG GGTCATTCTA CAGGCTGCCA ACTCAGACAT CCAGCAGGCC 
TGGGTGCAGG ACATCAACCA AGTCCTAGAA ACACAGAGGG ACTTCTTAAA TGCACTACAG 
TCACCCATTG AGTATCAGCG GAAGGAGAGA AGCACAGCTG TGATCCGGTC CCAGCCCCCG 
AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC 
CCCAAGGGCT CCAGCTATAA CCCGCCTCTG CCACCCCTGA AGATATCTAC CTCTAATGGC 
AGTCCAGGGT TCGACTACCA TCAGCCTGGG GACAAGTTTG ACGCTAGCAA GCAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAGA AGTCATGCTC TTGGCACACT CTACGCATGA GAAAGCGGGC AGACGTGGAG 
AACTCGGGTA AAAATGAAGC CACAGGGCCT CGTAAACCCA AGGATATTCT GGGCAACAAA 
GTCTCTGTTA AAATCTTGAA TCCAAATTTC ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG 
CCCTTAGTAG ATGTGACCTG TTTGCTTGGA GACACAGTGC TACTGCAGTG TAAAGCCTGT 
GGGCGGCCAA AGCCCAGCAT CACCTGGAAG GGTCCGGATC AGAACATCCT AGACACTGAC 
AACAGTTCAG CAACATACAC CATCTCCTCC TGTGATTCTG GGGAAAGCAC CCTCAAGATC 
TGCAACCTGA TGCCCCAGGA TAGTGGGATT TATACCTGCA TAGCAGCAAA CGACCACGGG 
ACTGCATCCA CATCTGCTAC AGTTAAGGTG CAAGGTGTCC CCGCAGCCCC CAACCGCCCA 
ATCGCACAGG AGAGGAGCTG CACCTCCGTC ATCCTCCGCT GGCTGCCCCC AGCCAGCACC 
GGAAACTGCA CTATTTCGGG GTACACAGTG GAGTATCGCG AGGAAGGTTC CCAGGTCTGG 
CAGCAATCCG TAGCTTCGAC CTTGGACACT TACCTGGTCA TTGAAGACCT TAGTCCTGGG 
TGCCCTTATC AGTTCAGGGT CAGTGCCAGC AACCCCTGGG GAATCAGCCT TCCCAGTGAG 
CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC GATGCAGCCG CCGACGGTGC CACCATTTCT 
TGGAAGGAAA ATTTTGATTC TGCTTACACT GAACTGAACG AGATTGGCAG AGGCCGTTTC 
TCTATTGTAA AGAAATGCAT TCACAAAGCT ACCCGCAAAG ATGTTGCTGT GAAATTTGTG 
AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT GCTCACGAGG CCGCCCTGCT CCAGCACCTG 
CAGCACCCTC AGTATGTCAC GCTCCATGAC ACCTACGAGT CCCCCACATC CTACATCCTG 
ATTTTGGAAC TGATGGATGA CGGCCGACTC TTGGACTACC TTATGAATCA TGATGAGCTG 
ATGGAAGAAA AGGTAGCTTT CTACATTCGA GACATCATGG AAGCTCTGCA GTACCTGCAC 
AACTGTAGGG TCGCACATTT GGACATAAAG CCCGAAAACC TGCTCATTGA CCTCCGGATC 
CCAGTGCCTC GAGTGAAGCT CATCGACTTG GAAGACGCTG TCCAGATCTC AGGCCACTTC 
CATATCCACC ATCTGCTGGG GAACCCTGAG TTCGCTGCCC CCGAAGTGAT CCAAGGCATC 
CCTGTCTCCC TGGGCACAGA CATCTGGAGC ATCGGGGTTC TGACATATGT CATGCTTAGT 
GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA GAGGAAACCT GTATCAACGT GTGCAGGGTG 
GACTTTAGCT TCCCCCACGA GTACTTCTGC GGTGTGAGCA ATGCTGCCAG GGATTTCATC 
AACGTAATCT TACAGGAAGA CTTCCGGAGG CGGCCCACAG CAGCCACGTG CCTGCAGCAT 
CCCTGGCTAC AGCCCCACAA CGGCAGCTAC TCCAAGATCC CCTTGGACAC CTCCCGTCTG 
GCCTGCTTCA TAGAACGCCG CAAGCACCAG AATGATGTGC GGCCCATTCC CAATGTCAAG 
AGTTACATCG TCAATAGGGT GAACCAAGGG ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_006522386.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522449.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X44, mRNA.

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CloneID	OMu52302
Clone ID Related Accession (Same CDS sequence)	XM_006522386.3 , XM_006522386.4
Accession Version	XM_006522386.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X40
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522386.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGAA GACGCACCAG 
AGCGCCCTGC AGGTACAGCA GAAGGCAGAG GCACTGCTCC AGGCCGGCCA CTACGACGCA 
GACGCCATCC GGGAATGTGC TGAGAAGGTG GCCCTCCACT GGCAACAGCT CATGCTAAAG 
ATGGAGGACC GGCTGAAGCT TGTCAACGCC TCTGTGGCCT TTTACAAGAC CTCTGAGCAG 
GTGTGCAGTG TTCTGGAGAG CTTGGAGCAG GAGTACCGGA GAGACGAGGA CTGGTGTGGT 
GGGAGAGACA AGCTGGGGCC GGCGGCAGAG ATGGACCACG TTATTCCACT CCTCAGCAAG 
CACCTGGAGC AGAAGGAGGC CTTCCTGAAG GCCTGCACGC TGGCAAGGCG GAACGCCGAG 
GTGTTCCTCA AATACATCCA CAGGAACAAT GTGAGCATGC CTAGTGTTGC CAGCCACACC 
CGGGGGCCTG AACAGCAAGT AAAAGCCATC TTGAGCGAGC TCTTGCAGAG GGAGAATCGA 
GTCCTGCACT TCTGGACCTT GAAGAAGCGG CGGTTAGACC AGTGTCAGCA ATATGTGGTG 
TTTGAGCGCA GTGCTAAGCA GGCACTGGAC TGGATCCAAG AAACAGGAGA ATATTACCTC 
TCAACACATA CCTCCACCGG AGAGACCACG GAGGAGACTC AGGAGCTGCT GAAAGAGTAT 
GGAGAATTCA GGGTGCCTGC CAAGCAAACG AAGGAGAAGG TGAAACTTCT GATCCAGCTA 
GCAGACAGCT TTGTGGAAAA GGGCCACATC CACGCCACAG AGATACGGAA ATGGGTGACC 
ACGGTGGACA AGCACTACCG CGATTTCTCC CTGAGGATGG GGAAGTACCG GTACTCGCTG 
GAGAAGGCGC TGGGGGTCAA CACGGAGGAT AACAAGGACC TAGAGCTGGA CATCATCCCA 
GCAAGCCTTT CGGATCGGGA GGTCAAGCTC CGGGACGCTA ACCATGAGAT CAATGAAGAG 
AAACGGAAGT CAGCTCGAAA GAAAGAGTTC ATTATGGCTG AACTGCTCCA AACTGAGAAG 
GCTTATGTAA GGGACTTGCA CGAGTGCTTG GAGACCTACC TGTGGGAGAT GACTAGTGGT 
GTGGAAGAGA TACCCCCCGG CATCCTCAAT AAGGAACATA TCATCTTCGG CAACATCCAG 
GAGATCTATG ACTTCCATAA CAACATCTTC CTCAAAGAGC TGGAGAAGTA CGAGCAGCTG 
CCTGAGGATG TGGGACACTG CTTTGTTACC TGGGCAGACA AATTTCAGAT GTATGTCACC 
TACTGTAAAA ACAAGCCTGA TTCCAACCAG CTGATCCTGG AGCATGCGGG CACCTTCTTT 
GATGAGATCC AGCAGCGGCA TGGCCTGGCC AACTCCATCT CCTCCTACCT AATTAAGCCT 
GTCCAGAGGG TTACCAAGTA CCAGCTGCTC CTGAAGGAAC TTCTAACTTG CTGTGAAGAA 
GGGAAAGGAG AGCTGAAGGA TGGCCTGGAG GTGATGCTCA GTGTCCCCAA GAAAGCCAAC 
GATGCCATGC ATGTCAGCAT GCTGGAAGGG TTCGATGAAA ACTTGGATGT ACAGGGGGAG 
TTGATTCTCC AGGATGCCTT TCAAGTGTGG GACCCAAAGT CGCTGATCCG GAAGGGGCGG 
GAGCGCCATC TGTTCCTCTT TGAGATCTCC TTGGTGTTTA GCAAAGAGAT CAAAGACTCT 
TCAGGACACA CGAAATATGT TTACAAGAAC AAGCTACTGA CCTCAGAGCT GGGTGTGACC 
GAGCATGTAG AAGGTGACCC CTGCAAATTC GCCTTGTGGT CTGGGCGCAC CCCATCCTCA 
GACAATAAAA CAGTGCTCAA AGCTTCCAAC ATCGAAACCA AGCAGGAATG GATCAAGAAC 
ATTCGAGAAG TGATTCAGGA GAGGATCATT CATCTTAAAG GCGCTTTAAA GGAGCCAATT 
CAGCTCCCCA AAACACCAGC CAAACTGAGG AACAATAGTA AGAGGGATGG GGTAGAGGAT 
GGTGACAGCC AAGGGGATGG GAGCAGCCAG CCAGATACCA TATCCATTGC CTCCAGGACC 
TCTCAGAACA CAGTGGAAAG TGACAAGCTC TCAGGTGGAT GTGAGCTGAC CGTGGTCCTC 
CAGGACTTCA GCGCAGGCCA CAGCAGCGAG CTGAGCATCC AGGTGGGCCA GACGGTGGAG 
CTGCTGGAAC GACCGAGTGA GAGGCCTGGC TGGTGTCTGG TCCGCACCAC AGAGCGGAGC 
CCCCCACAGG AAGGCCTGGT ACCCAGCAGC GCCCTGTGCA TCTCACACTC TCGCAGCAGC 
GTGGAAATGG ACTGCTTCTT CCCCTTGAAA GATTCATACT CTCATTCCTC CAGTGAGAAT 
GGAGGCAAAT CCGAGTCCGT GGCCCACCTG CAGTCTCAGC CCTCCCTGAA CTCCATCCAC 
AGTTCCCCTG GTCCCAAGCG CTCCACCAAC ACCCTCAAAA AGTGGCTGAC CAGCCCCGTG 
CGCCGCCTCA ACAGCGGAAA AGCCGACGGG AACATCAAAA AGCAGAAGAA AGTCCGGGAT 
GGGCGGAAGA GCTTCGATCT GGGATCGCCC AAGCCTGGGG ATGAGACGAC ACCTCAGGGA 
GACAGCGCTG ACGAGAAGAG CAAGAAGGGT TGGGGCGAAG ACGAGCCAGA TGAAGAGTCA 
CACACACCCC TCCCTCCGCC CATGAAGATC TTTGACAACG ACCCCACACA GGATGAGATG 
TCGTCTTTGC TAGCAGCCCG GCAGGCTCCC CCTGACGTGC CTACTGCTGC AGACCTTGTC 
AGTGCAATAG AAAAGTTGGT CAAAAATAAG CTGACTCTGG AAGGAGGCTC TTACCGGGGG 
AGCTTGAAGG ACCCTACAGG CTGCCTGAAT GAGGGGATGA CCCCTCCCAC ACCTCCTAGA 
AACCTGGAAG AAGAACAGAA AGCCAAGGCG CTGAGAGGCA GGATGTTTGT CCTGAATGAG 
CTGGTACAGA CAGAGAAGGA CTACGTCAAG GACCTGGGGA TTGTGGTGGA GGGCTTCATG 
AAGAGAATAG AAGAGAAGGG GGTCCCTGAG GACATGCGAG GGAAGGAAAA GATCGTGTTT 
GGAAATATCC ACCAGATTTA CGACTGGCAT AAAGATTTTT TCCTGGCTGA ACTGGAAAAA 
TGTATCCAAG AGCAAGACAG ATTGGCCCAG CTCTTCATTA AACACGAGCG GAAGCTGCAC 
ATCTACGTGT GGTACTGCCA GAACAAGCCG CGTTCCGAGT ACATCGTGGC TGAGTACGAT 
GCCTATTTTG AGGAGGTTAA ACAAGAGATC AATCAAAGGC TGACCCTTAG TGACTTCCTG 
ATCAAGCCCA TTCAGAGAAT AACAAAGTAC CAGCTGCTAC TCAAGGACTT CCTGCGATAC 
AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT ATTGAGAAAG CAGTGGAGTT AATGTGCCTT 
GTCCCCAAGC GCTGTAATGA CATGATGAAT CTGGGACGCC TGCAGGGCTT CGAGGGCACC 
CTGACCGCCC AGGGCAAGCT ACTGCAGCAG GACACATTCT ATGTGATCGA GCTGGATGCC 
GGCATGCAGT CCCGGACCAA GGAGAGGCGT GTGTTTCTCT TTGAGCAGAT TGTCATCTTC 
AGTGAACTGC TCCGAAAGGG GTCCCTCACC CCAGGCTACA TGTTCAAAAG AAGCATCAAG 
ATGAATTACT TGGTCCTGGA AGACAATGTG GATGGTGACC CCTGCAAATT TGCACTCATG 
AACAGGGAGA CTTCAGAAAG GGTCATTCTA CAGGCTGCCA ACTCAGACAT CCAGCAGGCC 
TGGGTGCAGG ACATCAACCA AGTCCTAGAA ACACAGAGGG ACTTCTTAAA TGCACTACAG 
TCACCCATTG AGTATCAGCG GAAGGAGAGA AGCACAGCTG TGATCCGGTC CCAGCCCCCG 
AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC 
CCCAAGGGCT CCAGCTATAA CCCGCCTCTG CCACCCCTGA AGATATCTAC CTCTAATGGC 
AGTCCAGGGT TCGACTACCA TCAGCCTGGG GACAAGTTTG ACGCTAGCAA GCAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAGA AGTCATGCTC TTGGCACACT CTACGCATGA GAAAGCGGGC AGACGTGGAG 
AACTCGGGTA AAAATGAAGC CACAGGGCCT CGTAAACCCA AGGATATTCT GGGCAACAAA 
GTCTCTGTTA AAATCTTGAA TCCAAATTTC ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG 
CCCTTAGTAG ATGTGACCTG TTTGCTTGGA GACACAGTGC TACTGCAGTG TAAAGCCTGT 
GGGCGGCCAA AGCCCAGCAT CACCTGGAAG GGTCCGGATC AGAACATCCT AGACACTGAC 
AACAGTTCAG CAACATACAC CATCTCCTCC TGTGATTCTG GGGAAAGCAC CCTCAAGATC 
TGCAACCTGA TGCCCCAGGA TAGTGGGATT TATACCTGCA TAGCAGCAAA CGACCACGGG 
ACTGCATCCA CATCTGCTAC AGTTAAGGTG CAAGGTGTCC CCGCAGCCCC CAACCGCCCA 
ATCGCACAGG AGAGGAGCTG CACCTCCGTC ATCCTCCGCT GGCTGCCCCC AGCCAGCACC 
GGAAACTGCA CTATTTCGGG GTACACAGTG GAGTATCGCG AGGAAGGTTC CCAGGTCTGG 
CAGCAATCCG TAGCTTCGAC CTTGGACACT TACCTGGTCA TTGAAGACCT TAGTCCTGGG 
TGCCCTTATC AGTTCAGGGT CAGTGCCAGC AACCCCTGGG GAATCAGCCT TCCCAGTGAG 
CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC GATGCAGCCG CCGACGGTGC CACCATTTCT 
TGGAAGGAAA ATTTTGATTC TGCTTACACT GAACTGAACG AGATTGGCAG AGGCCGTTTC 
TCTATTGTAA AGAAATGCAT TCACAAAGCT ACCCGCAAAG ATGTTGCTGT GAAATTTGTG 
AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT GCTCACGAGG CCGCCCTGCT CCAGCACCTG 
CAGCACCCTC AGTATGTCAC GCTCCATGAC ACCTACGAGT CCCCCACATC CTACATCCTG 
ATTTTGGAAC TGATGGATGA CGGCCGACTC TTGGACTACC TTATGAATCA TGATGAGCTG 
ATGGAAGAAA AGGTAGCTTT CTACATTCGA GACATCATGG AAGCTCTGCA GTACCTGCAC 
AACTGTAGGG TCGCACATTT GGACATAAAG CCCGAAAACC TGCTCATTGA CCTCCGGATC 
CCAGTGCCTC GAGTGAAGCT CATCGACTTG GAAGACGCTG TCCAGATCTC AGGCCACTTC 
CATATCCACC ATCTGCTGGG GAACCCTGAG TTCGCTGCCC CCGAAGTGAT CCAAGGCATC 
CCTGTCTCCC TGGGCACAGA CATCTGGAGC ATCGGGGTTC TGACATATGT CATGCTTAGT 
GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA GAGGAAACCT GTATCAACGT GTGCAGGGTG 
GACTTTAGCT TCCCCCACGA GTACTTCTGC GGTGTGAGCA ATGCTGCCAG GGATTTCATC 
AACGTAATCT TACAGGAAGA CTTCCGGAGG CGGCCCACAG CAGCCACGTG CCTGCAGCAT 
CCCTGGCTAC AGCCCCACAA CGGCAGCTAC TCCAAGATCC CCTTGGACAC CTCCCGTCTG 
GCCTGCTTCA TAGAACGCCG CAAGCACCAG AATGATGTGC GGCCCATTCC CAATGTCAAG 
AGTTACATCG TCAATAGGGT GAACCAAGGG ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522449.1
CDS	24875..33748
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_006522386.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X44, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522386.4

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGAA GACGCACCAG 
AGCGCCCTGC AGGTACAGCA GAAGGCAGAG GCACTGCTCC AGGCCGGCCA CTACGACGCA 
GACGCCATCC GGGAATGTGC TGAGAAGGTG GCCCTCCACT GGCAACAGCT CATGCTAAAG 
ATGGAGGACC GGCTGAAGCT TGTCAACGCC TCTGTGGCCT TTTACAAGAC CTCTGAGCAG 
GTGTGCAGTG TTCTGGAGAG CTTGGAGCAG GAGTACCGGA GAGACGAGGA CTGGTGTGGT 
GGGAGAGACA AGCTGGGGCC GGCGGCAGAG ATGGACCACG TTATTCCACT CCTCAGCAAG 
CACCTGGAGC AGAAGGAGGC CTTCCTGAAG GCCTGCACGC TGGCAAGGCG GAACGCCGAG 
GTGTTCCTCA AATACATCCA CAGGAACAAT GTGAGCATGC CTAGTGTTGC CAGCCACACC 
CGGGGGCCTG AACAGCAAGT AAAAGCCATC TTGAGCGAGC TCTTGCAGAG GGAGAATCGA 
GTCCTGCACT TCTGGACCTT GAAGAAGCGG CGGTTAGACC AGTGTCAGCA ATATGTGGTG 
TTTGAGCGCA GTGCTAAGCA GGCACTGGAC TGGATCCAAG AAACAGGAGA ATATTACCTC 
TCAACACATA CCTCCACCGG AGAGACCACG GAGGAGACTC AGGAGCTGCT GAAAGAGTAT 
GGAGAATTCA GGGTGCCTGC CAAGCAAACG AAGGAGAAGG TGAAACTTCT GATCCAGCTA 
GCAGACAGCT TTGTGGAAAA GGGCCACATC CACGCCACAG AGATACGGAA ATGGGTGACC 
ACGGTGGACA AGCACTACCG CGATTTCTCC CTGAGGATGG GGAAGTACCG GTACTCGCTG 
GAGAAGGCGC TGGGGGTCAA CACGGAGGAT AACAAGGACC TAGAGCTGGA CATCATCCCA 
GCAAGCCTTT CGGATCGGGA GGTCAAGCTC CGGGACGCTA ACCATGAGAT CAATGAAGAG 
AAACGGAAGT CAGCTCGAAA GAAAGAGTTC ATTATGGCTG AACTGCTCCA AACTGAGAAG 
GCTTATGTAA GGGACTTGCA CGAGTGCTTG GAGACCTACC TGTGGGAGAT GACTAGTGGT 
GTGGAAGAGA TACCCCCCGG CATCCTCAAT AAGGAACATA TCATCTTCGG CAACATCCAG 
GAGATCTATG ACTTCCATAA CAACATCTTC CTCAAAGAGC TGGAGAAGTA CGAGCAGCTG 
CCTGAGGATG TGGGACACTG CTTTGTTACC TGGGCAGACA AATTTCAGAT GTATGTCACC 
TACTGTAAAA ACAAGCCTGA TTCCAACCAG CTGATCCTGG AGCATGCGGG CACCTTCTTT 
GATGAGATCC AGCAGCGGCA TGGCCTGGCC AACTCCATCT CCTCCTACCT AATTAAGCCT 
GTCCAGAGGG TTACCAAGTA CCAGCTGCTC CTGAAGGAAC TTCTAACTTG CTGTGAAGAA 
GGGAAAGGAG AGCTGAAGGA TGGCCTGGAG GTGATGCTCA GTGTCCCCAA GAAAGCCAAC 
GATGCCATGC ATGTCAGCAT GCTGGAAGGG TTCGATGAAA ACTTGGATGT ACAGGGGGAG 
TTGATTCTCC AGGATGCCTT TCAAGTGTGG GACCCAAAGT CGCTGATCCG GAAGGGGCGG 
GAGCGCCATC TGTTCCTCTT TGAGATCTCC TTGGTGTTTA GCAAAGAGAT CAAAGACTCT 
TCAGGACACA CGAAATATGT TTACAAGAAC AAGCTACTGA CCTCAGAGCT GGGTGTGACC 
GAGCATGTAG AAGGTGACCC CTGCAAATTC GCCTTGTGGT CTGGGCGCAC CCCATCCTCA 
GACAATAAAA CAGTGCTCAA AGCTTCCAAC ATCGAAACCA AGCAGGAATG GATCAAGAAC 
ATTCGAGAAG TGATTCAGGA GAGGATCATT CATCTTAAAG GCGCTTTAAA GGAGCCAATT 
CAGCTCCCCA AAACACCAGC CAAACTGAGG AACAATAGTA AGAGGGATGG GGTAGAGGAT 
GGTGACAGCC AAGGGGATGG GAGCAGCCAG CCAGATACCA TATCCATTGC CTCCAGGACC 
TCTCAGAACA CAGTGGAAAG TGACAAGCTC TCAGGTGGAT GTGAGCTGAC CGTGGTCCTC 
CAGGACTTCA GCGCAGGCCA CAGCAGCGAG CTGAGCATCC AGGTGGGCCA GACGGTGGAG 
CTGCTGGAAC GACCGAGTGA GAGGCCTGGC TGGTGTCTGG TCCGCACCAC AGAGCGGAGC 
CCCCCACAGG AAGGCCTGGT ACCCAGCAGC GCCCTGTGCA TCTCACACTC TCGCAGCAGC 
GTGGAAATGG ACTGCTTCTT CCCCTTGAAA GATTCATACT CTCATTCCTC CAGTGAGAAT 
GGAGGCAAAT CCGAGTCCGT GGCCCACCTG CAGTCTCAGC CCTCCCTGAA CTCCATCCAC 
AGTTCCCCTG GTCCCAAGCG CTCCACCAAC ACCCTCAAAA AGTGGCTGAC CAGCCCCGTG 
CGCCGCCTCA ACAGCGGAAA AGCCGACGGG AACATCAAAA AGCAGAAGAA AGTCCGGGAT 
GGGCGGAAGA GCTTCGATCT GGGATCGCCC AAGCCTGGGG ATGAGACGAC ACCTCAGGGA 
GACAGCGCTG ACGAGAAGAG CAAGAAGGGT TGGGGCGAAG ACGAGCCAGA TGAAGAGTCA 
CACACACCCC TCCCTCCGCC CATGAAGATC TTTGACAACG ACCCCACACA GGATGAGATG 
TCGTCTTTGC TAGCAGCCCG GCAGGCTCCC CCTGACGTGC CTACTGCTGC AGACCTTGTC 
AGTGCAATAG AAAAGTTGGT CAAAAATAAG CTGACTCTGG AAGGAGGCTC TTACCGGGGG 
AGCTTGAAGG ACCCTACAGG CTGCCTGAAT GAGGGGATGA CCCCTCCCAC ACCTCCTAGA 
AACCTGGAAG AAGAACAGAA AGCCAAGGCG CTGAGAGGCA GGATGTTTGT CCTGAATGAG 
CTGGTACAGA CAGAGAAGGA CTACGTCAAG GACCTGGGGA TTGTGGTGGA GGGCTTCATG 
AAGAGAATAG AAGAGAAGGG GGTCCCTGAG GACATGCGAG GGAAGGAAAA GATCGTGTTT 
GGAAATATCC ACCAGATTTA CGACTGGCAT AAAGATTTTT TCCTGGCTGA ACTGGAAAAA 
TGTATCCAAG AGCAAGACAG ATTGGCCCAG CTCTTCATTA AACACGAGCG GAAGCTGCAC 
ATCTACGTGT GGTACTGCCA GAACAAGCCG CGTTCCGAGT ACATCGTGGC TGAGTACGAT 
GCCTATTTTG AGGAGGTTAA ACAAGAGATC AATCAAAGGC TGACCCTTAG TGACTTCCTG 
ATCAAGCCCA TTCAGAGAAT AACAAAGTAC CAGCTGCTAC TCAAGGACTT CCTGCGATAC 
AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT ATTGAGAAAG CAGTGGAGTT AATGTGCCTT 
GTCCCCAAGC GCTGTAATGA CATGATGAAT CTGGGACGCC TGCAGGGCTT CGAGGGCACC 
CTGACCGCCC AGGGCAAGCT ACTGCAGCAG GACACATTCT ATGTGATCGA GCTGGATGCC 
GGCATGCAGT CCCGGACCAA GGAGAGGCGT GTGTTTCTCT TTGAGCAGAT TGTCATCTTC 
AGTGAACTGC TCCGAAAGGG GTCCCTCACC CCAGGCTACA TGTTCAAAAG AAGCATCAAG 
ATGAATTACT TGGTCCTGGA AGACAATGTG GATGGTGACC CCTGCAAATT TGCACTCATG 
AACAGGGAGA CTTCAGAAAG GGTCATTCTA CAGGCTGCCA ACTCAGACAT CCAGCAGGCC 
TGGGTGCAGG ACATCAACCA AGTCCTAGAA ACACAGAGGG ACTTCTTAAA TGCACTACAG 
TCACCCATTG AGTATCAGCG GAAGGAGAGA AGCACAGCTG TGATCCGGTC CCAGCCCCCG 
AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC 
CCCAAGGGCT CCAGCTATAA CCCGCCTCTG CCACCCCTGA AGATATCTAC CTCTAATGGC 
AGTCCAGGGT TCGACTACCA TCAGCCTGGG GACAAGTTTG ACGCTAGCAA GCAGAACGAC 
CTGGGAGGCT GCAATGGGAC ATCAACCATG ACTGTAATCA AAGATTACTA TGCACTGAAG 
GAGAATGAAA TCTGCGTGAG CCAAGGTGAG GTGGTACAGG TCCTCGCCGT CAACCAGCAG 
AACATGTGTC TGGTGTACCA GCCTGCCAGC GACCATTCCC CGGCAGCAGA GGGCTGGGTC 
CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA GCCACAGCAG CGGCCGAAAG TAGTGACGGG 
AGCATCAAGA AGTCATGCTC TTGGCACACT CTACGCATGA GAAAGCGGGC AGACGTGGAG 
AACTCGGGTA AAAATGAAGC CACAGGGCCT CGTAAACCCA AGGATATTCT GGGCAACAAA 
GTCTCTGTTA AAATCTTGAA TCCAAATTTC ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG 
CCCTTAGTAG ATGTGACCTG TTTGCTTGGA GACACAGTGC TACTGCAGTG TAAAGCCTGT 
GGGCGGCCAA AGCCCAGCAT CACCTGGAAG GGTCCGGATC AGAACATCCT AGACACTGAC 
AACAGTTCAG CAACATACAC CATCTCCTCC TGTGATTCTG GGGAAAGCAC CCTCAAGATC 
TGCAACCTGA TGCCCCAGGA TAGTGGGATT TATACCTGCA TAGCAGCAAA CGACCACGGG 
ACTGCATCCA CATCTGCTAC AGTTAAGGTG CAAGGTGTCC CCGCAGCCCC CAACCGCCCA 
ATCGCACAGG AGAGGAGCTG CACCTCCGTC ATCCTCCGCT GGCTGCCCCC AGCCAGCACC 
GGAAACTGCA CTATTTCGGG GTACACAGTG GAGTATCGCG AGGAAGGTTC CCAGGTCTGG 
CAGCAATCCG TAGCTTCGAC CTTGGACACT TACCTGGTCA TTGAAGACCT TAGTCCTGGG 
TGCCCTTATC AGTTCAGGGT CAGTGCCAGC AACCCCTGGG GAATCAGCCT TCCCAGTGAG 
CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC GATGCAGCCG CCGACGGTGC CACCATTTCT 
TGGAAGGAAA ATTTTGATTC TGCTTACACT GAACTGAACG AGATTGGCAG AGGCCGTTTC 
TCTATTGTAA AGAAATGCAT TCACAAAGCT ACCCGCAAAG ATGTTGCTGT GAAATTTGTG 
AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT GCTCACGAGG CCGCCCTGCT CCAGCACCTG 
CAGCACCCTC AGTATGTCAC GCTCCATGAC ACCTACGAGT CCCCCACATC CTACATCCTG 
ATTTTGGAAC TGATGGATGA CGGCCGACTC TTGGACTACC TTATGAATCA TGATGAGCTG 
ATGGAAGAAA AGGTAGCTTT CTACATTCGA GACATCATGG AAGCTCTGCA GTACCTGCAC 
AACTGTAGGG TCGCACATTT GGACATAAAG CCCGAAAACC TGCTCATTGA CCTCCGGATC 
CCAGTGCCTC GAGTGAAGCT CATCGACTTG GAAGACGCTG TCCAGATCTC AGGCCACTTC 
CATATCCACC ATCTGCTGGG GAACCCTGAG TTCGCTGCCC CCGAAGTGAT CCAAGGCATC 
CCTGTCTCCC TGGGCACAGA CATCTGGAGC ATCGGGGTTC TGACATATGT CATGCTTAGT 
GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA GAGGAAACCT GTATCAACGT GTGCAGGGTG 
GACTTTAGCT TCCCCCACGA GTACTTCTGC GGTGTGAGCA ATGCTGCCAG GGATTTCATC 
AACGTAATCT TACAGGAAGA CTTCCGGAGG CGGCCCACAG CAGCCACGTG CCTGCAGCAT 
CCCTGGCTAC AGCCCCACAA CGGCAGCTAC TCCAAGATCC CCTTGGACAC CTCCCGTCTG 
GCCTGCTTCA TAGAACGCCG CAAGCACCAG AATGATGTGC GGCCCATTCC CAATGTCAAG 
AGTTACATCG TCAATAGGGT GAACCAAGGG ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245951.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244253.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X1, mRNA.

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CloneID	OMu75307
Clone ID Related Accession (Same CDS sequence)	XM_011245951.2 , XM_011245951.3
Accession Version	XM_011245951.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245951.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAGAAGTC ATGCTCTTGG CACACTCTAC GCATGAGAAA GCGGGCAGAC 
GTGGAGAACT CGGGTAAAAA TGAAGCCACA GGGCCTCGTA AACCCAAGGA TATTCTGGGC 
AACAAAGTCT CTGTTAAAGA GACGAACAGT TCCGAGGAGT CAGAGTGTGA TGATCTTGAC 
CCTAATACTA GTATGGAGAT CTTGAATCCA AATTTCATCC AGGAAGTGGC TCCAGAATTC 
CTCGTGCCCT TAGTAGATGT GACCTGTTTG CTTGGAGACA CAGTGCTACT GCAGTGTAAA 
GCCTGTGGGC GGCCAAAGCC CAGCATCACC TGGAAGGGTC CGGATCAGAA CATCCTAGAC 
ACTGACAACA GTTCAGCAAC ATACACCATC TCCTCCTGTG ATTCTGGGGA AAGCACCCTC 
AAGATCTGCA ACCTGATGCC CCAGGATAGT GGGATTTATA CCTGCATAGC AGCAAACGAC 
CACGGGACTG CATCCACATC TGCTACAGTT AAGGTGCAAG GTGTCCCCGC AGCCCCCAAC 
CGCCCAATCG CACAGGAGAG GAGCTGCACC TCCGTCATCC TCCGCTGGCT GCCCCCAGCC 
AGCACCGGAA ACTGCACTAT TTCGGGGTAC ACAGTGGAGT ATCGCGAGGA AGGTTCCCAG 
GTCTGGCAGC AATCCGTAGC TTCGACCTTG GACACTTACC TGGTCATTGA AGACCTTAGT 
CCTGGGTGCC CTTATCAGTT CAGGGTCAGT GCCAGCAACC CCTGGGGAAT CAGCCTTCCC 
AGTGAGCCCT CGGAGTTTGT GCGCCTTCCA GAGTACGATG CAGCCGCCGA CGGTGCCACC 
ATTTCTTGGA AGGAAAATTT TGATTCTGCT TACACTGAAC TGAACGAGAT TGGCAGAGGC 
CGTTTCTCTA TTGTAAAGAA ATGCATTCAC AAAGCTACCC GCAAAGATGT TGCTGTGAAA 
TTTGTGAGCA AAAAAATGAA GAAGAAGGAG CAGGCTGCTC ACGAGGCCGC CCTGCTCCAG 
CACCTGCAGC ACCCTCAGTA TGTCACGCTC CATGACACCT ACGAGTCCCC CACATCCTAC 
ATCCTGATTT TGGAACTGAT GGATGACGGC CGACTCTTGG ACTACCTTAT GAATCATGAT 
GAGCTGATGG AAGAAAAGGT AGCTTTCTAC ATTCGAGACA TCATGGAAGC TCTGCAGTAC 
CTGCACAACT GTAGGGTCGC ACATTTGGAC ATAAAGCCCG AAAACCTGCT CATTGACCTC 
CGGATCCCAG TGCCTCGAGT GAAGCTCATC GACTTGGAAG ACGCTGTCCA GATCTCAGGC 
CACTTCCATA TCCACCATCT GCTGGGGAAC CCTGAGTTCG CTGCCCCCGA AGTGATCCAA 
GGCATCCCTG TCTCCCTGGG CACAGACATC TGGAGCATCG GGGTTCTGAC ATATGTCATG 
CTTAGTGGGG TCTCCCCCTT CTTAGATGAG AGCAAAGAGG AAACCTGTAT CAACGTGTGC 
AGGGTGGACT TTAGCTTCCC CCACGAGTAC TTCTGCGGTG TGAGCAATGC TGCCAGGGAT 
TTCATCAACG TAATCTTACA GGAAGACTTC CGGAGGCGGC CCACAGCAGC CACGTGCCTG 
CAGCATCCCT GGCTACAGCC CCACAACGGC AGCTACTCCA AGATCCCCTT GGACACCTCC 
CGTCTGGCCT GCTTCATAGA ACGCCGCAAG CACCAGAATG ATGTGCGGCC CATTCCCAAT 
GTCAAGAGTT ACATCGTCAA TAGGGTGAAC CAAGGGACCT CGCTATCCCA CAACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244253.1
CDS	4891..13890
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245951.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245951.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAGAAGTC ATGCTCTTGG CACACTCTAC GCATGAGAAA GCGGGCAGAC 
GTGGAGAACT CGGGTAAAAA TGAAGCCACA GGGCCTCGTA AACCCAAGGA TATTCTGGGC 
AACAAAGTCT CTGTTAAAGA GACGAACAGT TCCGAGGAGT CAGAGTGTGA TGATCTTGAC 
CCTAATACTA GTATGGAGAT CTTGAATCCA AATTTCATCC AGGAAGTGGC TCCAGAATTC 
CTCGTGCCCT TAGTAGATGT GACCTGTTTG CTTGGAGACA CAGTGCTACT GCAGTGTAAA 
GCCTGTGGGC GGCCAAAGCC CAGCATCACC TGGAAGGGTC CGGATCAGAA CATCCTAGAC 
ACTGACAACA GTTCAGCAAC ATACACCATC TCCTCCTGTG ATTCTGGGGA AAGCACCCTC 
AAGATCTGCA ACCTGATGCC CCAGGATAGT GGGATTTATA CCTGCATAGC AGCAAACGAC 
CACGGGACTG CATCCACATC TGCTACAGTT AAGGTGCAAG GTGTCCCCGC AGCCCCCAAC 
CGCCCAATCG CACAGGAGAG GAGCTGCACC TCCGTCATCC TCCGCTGGCT GCCCCCAGCC 
AGCACCGGAA ACTGCACTAT TTCGGGGTAC ACAGTGGAGT ATCGCGAGGA AGGTTCCCAG 
GTCTGGCAGC AATCCGTAGC TTCGACCTTG GACACTTACC TGGTCATTGA AGACCTTAGT 
CCTGGGTGCC CTTATCAGTT CAGGGTCAGT GCCAGCAACC CCTGGGGAAT CAGCCTTCCC 
AGTGAGCCCT CGGAGTTTGT GCGCCTTCCA GAGTACGATG CAGCCGCCGA CGGTGCCACC 
ATTTCTTGGA AGGAAAATTT TGATTCTGCT TACACTGAAC TGAACGAGAT TGGCAGAGGC 
CGTTTCTCTA TTGTAAAGAA ATGCATTCAC AAAGCTACCC GCAAAGATGT TGCTGTGAAA 
TTTGTGAGCA AAAAAATGAA GAAGAAGGAG CAGGCTGCTC ACGAGGCCGC CCTGCTCCAG 
CACCTGCAGC ACCCTCAGTA TGTCACGCTC CATGACACCT ACGAGTCCCC CACATCCTAC 
ATCCTGATTT TGGAACTGAT GGATGACGGC CGACTCTTGG ACTACCTTAT GAATCATGAT 
GAGCTGATGG AAGAAAAGGT AGCTTTCTAC ATTCGAGACA TCATGGAAGC TCTGCAGTAC 
CTGCACAACT GTAGGGTCGC ACATTTGGAC ATAAAGCCCG AAAACCTGCT CATTGACCTC 
CGGATCCCAG TGCCTCGAGT GAAGCTCATC GACTTGGAAG ACGCTGTCCA GATCTCAGGC 
CACTTCCATA TCCACCATCT GCTGGGGAAC CCTGAGTTCG CTGCCCCCGA AGTGATCCAA 
GGCATCCCTG TCTCCCTGGG CACAGACATC TGGAGCATCG GGGTTCTGAC ATATGTCATG 
CTTAGTGGGG TCTCCCCCTT CTTAGATGAG AGCAAAGAGG AAACCTGTAT CAACGTGTGC 
AGGGTGGACT TTAGCTTCCC CCACGAGTAC TTCTGCGGTG TGAGCAATGC TGCCAGGGAT 
TTCATCAACG TAATCTTACA GGAAGACTTC CGGAGGCGGC CCACAGCAGC CACGTGCCTG 
CAGCATCCCT GGCTACAGCC CCACAACGGC AGCTACTCCA AGATCCCCTT GGACACCTCC 
CGTCTGGCCT GCTTCATAGA ACGCCGCAAG CACCAGAATG ATGTGCGGCC CATTCCCAAT 
GTCAAGAGTT ACATCGTCAA TAGGGTGAAC CAAGGGACCT CGCTATCCCA CAACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245951.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244253.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X1, mRNA.

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CloneID	OMu75307
Clone ID Related Accession (Same CDS sequence)	XM_011245951.2 , XM_011245951.3
Accession Version	XM_011245951.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAGAAGTC ATGCTCTTGG CACACTCTAC GCATGAGAAA GCGGGCAGAC 
GTGGAGAACT CGGGTAAAAA TGAAGCCACA GGGCCTCGTA AACCCAAGGA TATTCTGGGC 
AACAAAGTCT CTGTTAAAGA GACGAACAGT TCCGAGGAGT CAGAGTGTGA TGATCTTGAC 
CCTAATACTA GTATGGAGAT CTTGAATCCA AATTTCATCC AGGAAGTGGC TCCAGAATTC 
CTCGTGCCCT TAGTAGATGT GACCTGTTTG CTTGGAGACA CAGTGCTACT GCAGTGTAAA 
GCCTGTGGGC GGCCAAAGCC CAGCATCACC TGGAAGGGTC CGGATCAGAA CATCCTAGAC 
ACTGACAACA GTTCAGCAAC ATACACCATC TCCTCCTGTG ATTCTGGGGA AAGCACCCTC 
AAGATCTGCA ACCTGATGCC CCAGGATAGT GGGATTTATA CCTGCATAGC AGCAAACGAC 
CACGGGACTG CATCCACATC TGCTACAGTT AAGGTGCAAG GTGTCCCCGC AGCCCCCAAC 
CGCCCAATCG CACAGGAGAG GAGCTGCACC TCCGTCATCC TCCGCTGGCT GCCCCCAGCC 
AGCACCGGAA ACTGCACTAT TTCGGGGTAC ACAGTGGAGT ATCGCGAGGA AGGTTCCCAG 
GTCTGGCAGC AATCCGTAGC TTCGACCTTG GACACTTACC TGGTCATTGA AGACCTTAGT 
CCTGGGTGCC CTTATCAGTT CAGGGTCAGT GCCAGCAACC CCTGGGGAAT CAGCCTTCCC 
AGTGAGCCCT CGGAGTTTGT GCGCCTTCCA GAGTACGATG CAGCCGCCGA CGGTGCCACC 
ATTTCTTGGA AGGAAAATTT TGATTCTGCT TACACTGAAC TGAACGAGAT TGGCAGAGGC 
CGTTTCTCTA TTGTAAAGAA ATGCATTCAC AAAGCTACCC GCAAAGATGT TGCTGTGAAA 
TTTGTGAGCA AAAAAATGAA GAAGAAGGAG CAGGCTGCTC ACGAGGCCGC CCTGCTCCAG 
CACCTGCAGC ACCCTCAGTA TGTCACGCTC CATGACACCT ACGAGTCCCC CACATCCTAC 
ATCCTGATTT TGGAACTGAT GGATGACGGC CGACTCTTGG ACTACCTTAT GAATCATGAT 
GAGCTGATGG AAGAAAAGGT AGCTTTCTAC ATTCGAGACA TCATGGAAGC TCTGCAGTAC 
CTGCACAACT GTAGGGTCGC ACATTTGGAC ATAAAGCCCG AAAACCTGCT CATTGACCTC 
CGGATCCCAG TGCCTCGAGT GAAGCTCATC GACTTGGAAG ACGCTGTCCA GATCTCAGGC 
CACTTCCATA TCCACCATCT GCTGGGGAAC CCTGAGTTCG CTGCCCCCGA AGTGATCCAA 
GGCATCCCTG TCTCCCTGGG CACAGACATC TGGAGCATCG GGGTTCTGAC ATATGTCATG 
CTTAGTGGGG TCTCCCCCTT CTTAGATGAG AGCAAAGAGG AAACCTGTAT CAACGTGTGC 
AGGGTGGACT TTAGCTTCCC CCACGAGTAC TTCTGCGGTG TGAGCAATGC TGCCAGGGAT 
TTCATCAACG TAATCTTACA GGAAGACTTC CGGAGGCGGC CCACAGCAGC CACGTGCCTG 
CAGCATCCCT GGCTACAGCC CCACAACGGC AGCTACTCCA AGATCCCCTT GGACACCTCC 
CGTCTGGCCT GCTTCATAGA ACGCCGCAAG CACCAGAATG ATGTGCGGCC CATTCCCAAT 
GTCAAGAGTT ACATCGTCAA TAGGGTGAAC CAAGGGACCT CGCTATCCCA CAACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244253.1
CDS	24875..33874
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245951.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245951.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAGAAGTC ATGCTCTTGG CACACTCTAC GCATGAGAAA GCGGGCAGAC 
GTGGAGAACT CGGGTAAAAA TGAAGCCACA GGGCCTCGTA AACCCAAGGA TATTCTGGGC 
AACAAAGTCT CTGTTAAAGA GACGAACAGT TCCGAGGAGT CAGAGTGTGA TGATCTTGAC 
CCTAATACTA GTATGGAGAT CTTGAATCCA AATTTCATCC AGGAAGTGGC TCCAGAATTC 
CTCGTGCCCT TAGTAGATGT GACCTGTTTG CTTGGAGACA CAGTGCTACT GCAGTGTAAA 
GCCTGTGGGC GGCCAAAGCC CAGCATCACC TGGAAGGGTC CGGATCAGAA CATCCTAGAC 
ACTGACAACA GTTCAGCAAC ATACACCATC TCCTCCTGTG ATTCTGGGGA AAGCACCCTC 
AAGATCTGCA ACCTGATGCC CCAGGATAGT GGGATTTATA CCTGCATAGC AGCAAACGAC 
CACGGGACTG CATCCACATC TGCTACAGTT AAGGTGCAAG GTGTCCCCGC AGCCCCCAAC 
CGCCCAATCG CACAGGAGAG GAGCTGCACC TCCGTCATCC TCCGCTGGCT GCCCCCAGCC 
AGCACCGGAA ACTGCACTAT TTCGGGGTAC ACAGTGGAGT ATCGCGAGGA AGGTTCCCAG 
GTCTGGCAGC AATCCGTAGC TTCGACCTTG GACACTTACC TGGTCATTGA AGACCTTAGT 
CCTGGGTGCC CTTATCAGTT CAGGGTCAGT GCCAGCAACC CCTGGGGAAT CAGCCTTCCC 
AGTGAGCCCT CGGAGTTTGT GCGCCTTCCA GAGTACGATG CAGCCGCCGA CGGTGCCACC 
ATTTCTTGGA AGGAAAATTT TGATTCTGCT TACACTGAAC TGAACGAGAT TGGCAGAGGC 
CGTTTCTCTA TTGTAAAGAA ATGCATTCAC AAAGCTACCC GCAAAGATGT TGCTGTGAAA 
TTTGTGAGCA AAAAAATGAA GAAGAAGGAG CAGGCTGCTC ACGAGGCCGC CCTGCTCCAG 
CACCTGCAGC ACCCTCAGTA TGTCACGCTC CATGACACCT ACGAGTCCCC CACATCCTAC 
ATCCTGATTT TGGAACTGAT GGATGACGGC CGACTCTTGG ACTACCTTAT GAATCATGAT 
GAGCTGATGG AAGAAAAGGT AGCTTTCTAC ATTCGAGACA TCATGGAAGC TCTGCAGTAC 
CTGCACAACT GTAGGGTCGC ACATTTGGAC ATAAAGCCCG AAAACCTGCT CATTGACCTC 
CGGATCCCAG TGCCTCGAGT GAAGCTCATC GACTTGGAAG ACGCTGTCCA GATCTCAGGC 
CACTTCCATA TCCACCATCT GCTGGGGAAC CCTGAGTTCG CTGCCCCCGA AGTGATCCAA 
GGCATCCCTG TCTCCCTGGG CACAGACATC TGGAGCATCG GGGTTCTGAC ATATGTCATG 
CTTAGTGGGG TCTCCCCCTT CTTAGATGAG AGCAAAGAGG AAACCTGTAT CAACGTGTGC 
AGGGTGGACT TTAGCTTCCC CCACGAGTAC TTCTGCGGTG TGAGCAATGC TGCCAGGGAT 
TTCATCAACG TAATCTTACA GGAAGACTTC CGGAGGCGGC CCACAGCAGC CACGTGCCTG 
CAGCATCCCT GGCTACAGCC CCACAACGGC AGCTACTCCA AGATCCCCTT GGACACCTCC 
CGTCTGGCCT GCTTCATAGA ACGCCGCAAG CACCAGAATG ATGTGCGGCC CATTCCCAAT 
GTCAAGAGTT ACATCGTCAA TAGGGTGAAC CAAGGGACCT CGCTATCCCA CAACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245952.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244254.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X2, mRNA.

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CloneID	OMu75308
Clone ID Related Accession (Same CDS sequence)	XM_011245952.3 , XM_011245952.2
Accession Version	XM_011245952.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGTCGTCTT TGCTAGCAGC CCGGCAGGCT CCCCCTGACG TGCCTACTGC TGCAGACCTT 
GTCAGTGCAA TAGAAAAGTT GGTCAAAAAT AAGCTGACTC TGGAAGGAGG CTCTTACCGG 
GGGAGCTTGA AGGACCCTAC AGGCTGCCTG AATGAGGGGA TGACCCCTCC CACACCTCCT 
AGAAACCTGG AAGAAGAACA GAAAGCCAAG GCGCTGAGAG GCAGGATGTT TGTCCTGAAT 
GAGCTGGTAC AGACAGAGAA GGACTACGTC AAGGACCTGG GGATTGTGGT GGAGGGCTTC 
ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT GAGGACATGC GAGGGAAGGA AAAGATCGTG 
TTTGGAAATA TCCACCAGAT TTACGACTGG CATAAAGATT TTTTCCTGGC TGAACTGGAA 
AAATGTATCC AAGAGCAAGA CAGATTGGCC CAGCTCTTCA TTAAACACGA GCGGAAGCTG 
CACATCTACG TGTGGTACTG CCAGAACAAG CCGCGTTCCG AGTACATCGT GGCTGAGTAC 
GATGCCTATT TTGAGGAGGT TAAACAAGAG ATCAATCAAA GGCTGACCCT TAGTGACTTC 
CTGATCAAGC CCATTCAGAG AATAACAAAG TACCAGCTGC TACTCAAGGA CTTCCTGCGA 
TACAGCGAGA AGGCTGGTTT GGAGTGTTCC GATATTGAGA AAGCAGTGGA GTTAATGTGC 
CTTGTCCCCA AGCGCTGTAA TGACATGATG AATCTGGGAC GCCTGCAGGG CTTCGAGGGC 
ACCCTGACCG CCCAGGGCAA GCTACTGCAG CAGGACACAT TCTATGTGAT CGAGCTGGAT 
GCCGGCATGC AGTCCCGGAC CAAGGAGAGG CGTGTGTTTC TCTTTGAGCA GATTGTCATC 
TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC ACCCCAGGCT ACATGTTCAA AAGAAGCATC 
AAGATGAATT ACTTGGTCCT GGAAGACAAT GTGGATGGTG ACCCCTGCAA ATTTGCACTC 
ATGAACAGGG AGACTTCAGA AAGGGTCATT CTACAGGCTG CCAACTCAGA CATCCAGCAG 
GCCTGGGTGC AGGACATCAA CCAAGTCCTA GAAACACAGA GGGACTTCTT AAATGCACTA 
CAGTCACCCA TTGAGTATCA GCGGAAGGAG AGAAGCACAG CTGTGATCCG GTCCCAGCCC 
CCGAGGGTTC CACAAGCCAG CCCCAGGCCC TACTCTTCTG GCCCGGTGGG CTCAGAGAAG 
CCCCCCAAGG GCTCCAGCTA TAACCCGCCT CTGCCACCCC TGAAGATATC TACCTCTAAT 
GGCAGTCCAG GGTTCGACTA CCATCAGCCT GGGGACAAGT TTGACGCTAG CAAGCAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AGAAGTCATG CTCTTGGCAC ACTCTACGCA TGAGAAAGCG GGCAGACGTG 
GAGAACTCGG GTAAAAATGA AGCCACAGGG CCTCGTAAAC CCAAGGATAT TCTGGGCAAC 
AAAGTCTCTG TTAAAGAGAC GAACAGTTCC GAGGAGTCAG AGTGTGATGA TCTTGACCCT 
AATACTAGTA TGGAGATCTT GAATCCAAAT TTCATCCAGG AAGTGGCTCC AGAATTCCTC 
GTGCCCTTAG TAGATGTGAC CTGTTTGCTT GGAGACACAG TGCTACTGCA GTGTAAAGCC 
TGTGGGCGGC CAAAGCCCAG CATCACCTGG AAGGGTCCGG ATCAGAACAT CCTAGACACT 
GACAACAGTT CAGCAACATA CACCATCTCC TCCTGTGATT CTGGGGAAAG CACCCTCAAG 
ATCTGCAACC TGATGCCCCA GGATAGTGGG ATTTATACCT GCATAGCAGC AAACGACCAC 
GGGACTGCAT CCACATCTGC TACAGTTAAG GTGCAAGGTG TCCCCGCAGC CCCCAACCGC 
CCAATCGCAC AGGAGAGGAG CTGCACCTCC GTCATCCTCC GCTGGCTGCC CCCAGCCAGC 
ACCGGAAACT GCACTATTTC GGGGTACACA GTGGAGTATC GCGAGGAAGG TTCCCAGGTC 
TGGCAGCAAT CCGTAGCTTC GACCTTGGAC ACTTACCTGG TCATTGAAGA CCTTAGTCCT 
GGGTGCCCTT ATCAGTTCAG GGTCAGTGCC AGCAACCCCT GGGGAATCAG CCTTCCCAGT 
GAGCCCTCGG AGTTTGTGCG CCTTCCAGAG TACGATGCAG CCGCCGACGG TGCCACCATT 
TCTTGGAAGG AAAATTTTGA TTCTGCTTAC ACTGAACTGA ACGAGATTGG CAGAGGCCGT 
TTCTCTATTG TAAAGAAATG CATTCACAAA GCTACCCGCA AAGATGTTGC TGTGAAATTT 
GTGAGCAAAA AAATGAAGAA GAAGGAGCAG GCTGCTCACG AGGCCGCCCT GCTCCAGCAC 
CTGCAGCACC CTCAGTATGT CACGCTCCAT GACACCTACG AGTCCCCCAC ATCCTACATC 
CTGATTTTGG AACTGATGGA TGACGGCCGA CTCTTGGACT ACCTTATGAA TCATGATGAG 
CTGATGGAAG AAAAGGTAGC TTTCTACATT CGAGACATCA TGGAAGCTCT GCAGTACCTG 
CACAACTGTA GGGTCGCACA TTTGGACATA AAGCCCGAAA ACCTGCTCAT TGACCTCCGG 
ATCCCAGTGC CTCGAGTGAA GCTCATCGAC TTGGAAGACG CTGTCCAGAT CTCAGGCCAC 
TTCCATATCC ACCATCTGCT GGGGAACCCT GAGTTCGCTG CCCCCGAAGT GATCCAAGGC 
ATCCCTGTCT CCCTGGGCAC AGACATCTGG AGCATCGGGG TTCTGACATA TGTCATGCTT 
AGTGGGGTCT CCCCCTTCTT AGATGAGAGC AAAGAGGAAA CCTGTATCAA CGTGTGCAGG 
GTGGACTTTA GCTTCCCCCA CGAGTACTTC TGCGGTGTGA GCAATGCTGC CAGGGATTTC 
ATCAACGTAA TCTTACAGGA AGACTTCCGG AGGCGGCCCA CAGCAGCCAC GTGCCTGCAG 
CATCCCTGGC TACAGCCCCA CAACGGCAGC TACTCCAAGA TCCCCTTGGA CACCTCCCGT 
CTGGCCTGCT TCATAGAACG CCGCAAGCAC CAGAATGATG TGCGGCCCAT TCCCAATGTC 
AAGAGTTACA TCGTCAATAG GGTGAACCAA GGGACCTCGC TATCCCACAA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244254.1
CDS	24875..33871
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245952.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245952.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGTCGTCTT TGCTAGCAGC CCGGCAGGCT CCCCCTGACG TGCCTACTGC TGCAGACCTT 
GTCAGTGCAA TAGAAAAGTT GGTCAAAAAT AAGCTGACTC TGGAAGGAGG CTCTTACCGG 
GGGAGCTTGA AGGACCCTAC AGGCTGCCTG AATGAGGGGA TGACCCCTCC CACACCTCCT 
AGAAACCTGG AAGAAGAACA GAAAGCCAAG GCGCTGAGAG GCAGGATGTT TGTCCTGAAT 
GAGCTGGTAC AGACAGAGAA GGACTACGTC AAGGACCTGG GGATTGTGGT GGAGGGCTTC 
ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT GAGGACATGC GAGGGAAGGA AAAGATCGTG 
TTTGGAAATA TCCACCAGAT TTACGACTGG CATAAAGATT TTTTCCTGGC TGAACTGGAA 
AAATGTATCC AAGAGCAAGA CAGATTGGCC CAGCTCTTCA TTAAACACGA GCGGAAGCTG 
CACATCTACG TGTGGTACTG CCAGAACAAG CCGCGTTCCG AGTACATCGT GGCTGAGTAC 
GATGCCTATT TTGAGGAGGT TAAACAAGAG ATCAATCAAA GGCTGACCCT TAGTGACTTC 
CTGATCAAGC CCATTCAGAG AATAACAAAG TACCAGCTGC TACTCAAGGA CTTCCTGCGA 
TACAGCGAGA AGGCTGGTTT GGAGTGTTCC GATATTGAGA AAGCAGTGGA GTTAATGTGC 
CTTGTCCCCA AGCGCTGTAA TGACATGATG AATCTGGGAC GCCTGCAGGG CTTCGAGGGC 
ACCCTGACCG CCCAGGGCAA GCTACTGCAG CAGGACACAT TCTATGTGAT CGAGCTGGAT 
GCCGGCATGC AGTCCCGGAC CAAGGAGAGG CGTGTGTTTC TCTTTGAGCA GATTGTCATC 
TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC ACCCCAGGCT ACATGTTCAA AAGAAGCATC 
AAGATGAATT ACTTGGTCCT GGAAGACAAT GTGGATGGTG ACCCCTGCAA ATTTGCACTC 
ATGAACAGGG AGACTTCAGA AAGGGTCATT CTACAGGCTG CCAACTCAGA CATCCAGCAG 
GCCTGGGTGC AGGACATCAA CCAAGTCCTA GAAACACAGA GGGACTTCTT AAATGCACTA 
CAGTCACCCA TTGAGTATCA GCGGAAGGAG AGAAGCACAG CTGTGATCCG GTCCCAGCCC 
CCGAGGGTTC CACAAGCCAG CCCCAGGCCC TACTCTTCTG GCCCGGTGGG CTCAGAGAAG 
CCCCCCAAGG GCTCCAGCTA TAACCCGCCT CTGCCACCCC TGAAGATATC TACCTCTAAT 
GGCAGTCCAG GGTTCGACTA CCATCAGCCT GGGGACAAGT TTGACGCTAG CAAGCAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AGAAGTCATG CTCTTGGCAC ACTCTACGCA TGAGAAAGCG GGCAGACGTG 
GAGAACTCGG GTAAAAATGA AGCCACAGGG CCTCGTAAAC CCAAGGATAT TCTGGGCAAC 
AAAGTCTCTG TTAAAGAGAC GAACAGTTCC GAGGAGTCAG AGTGTGATGA TCTTGACCCT 
AATACTAGTA TGGAGATCTT GAATCCAAAT TTCATCCAGG AAGTGGCTCC AGAATTCCTC 
GTGCCCTTAG TAGATGTGAC CTGTTTGCTT GGAGACACAG TGCTACTGCA GTGTAAAGCC 
TGTGGGCGGC CAAAGCCCAG CATCACCTGG AAGGGTCCGG ATCAGAACAT CCTAGACACT 
GACAACAGTT CAGCAACATA CACCATCTCC TCCTGTGATT CTGGGGAAAG CACCCTCAAG 
ATCTGCAACC TGATGCCCCA GGATAGTGGG ATTTATACCT GCATAGCAGC AAACGACCAC 
GGGACTGCAT CCACATCTGC TACAGTTAAG GTGCAAGGTG TCCCCGCAGC CCCCAACCGC 
CCAATCGCAC AGGAGAGGAG CTGCACCTCC GTCATCCTCC GCTGGCTGCC CCCAGCCAGC 
ACCGGAAACT GCACTATTTC GGGGTACACA GTGGAGTATC GCGAGGAAGG TTCCCAGGTC 
TGGCAGCAAT CCGTAGCTTC GACCTTGGAC ACTTACCTGG TCATTGAAGA CCTTAGTCCT 
GGGTGCCCTT ATCAGTTCAG GGTCAGTGCC AGCAACCCCT GGGGAATCAG CCTTCCCAGT 
GAGCCCTCGG AGTTTGTGCG CCTTCCAGAG TACGATGCAG CCGCCGACGG TGCCACCATT 
TCTTGGAAGG AAAATTTTGA TTCTGCTTAC ACTGAACTGA ACGAGATTGG CAGAGGCCGT 
TTCTCTATTG TAAAGAAATG CATTCACAAA GCTACCCGCA AAGATGTTGC TGTGAAATTT 
GTGAGCAAAA AAATGAAGAA GAAGGAGCAG GCTGCTCACG AGGCCGCCCT GCTCCAGCAC 
CTGCAGCACC CTCAGTATGT CACGCTCCAT GACACCTACG AGTCCCCCAC ATCCTACATC 
CTGATTTTGG AACTGATGGA TGACGGCCGA CTCTTGGACT ACCTTATGAA TCATGATGAG 
CTGATGGAAG AAAAGGTAGC TTTCTACATT CGAGACATCA TGGAAGCTCT GCAGTACCTG 
CACAACTGTA GGGTCGCACA TTTGGACATA AAGCCCGAAA ACCTGCTCAT TGACCTCCGG 
ATCCCAGTGC CTCGAGTGAA GCTCATCGAC TTGGAAGACG CTGTCCAGAT CTCAGGCCAC 
TTCCATATCC ACCATCTGCT GGGGAACCCT GAGTTCGCTG CCCCCGAAGT GATCCAAGGC 
ATCCCTGTCT CCCTGGGCAC AGACATCTGG AGCATCGGGG TTCTGACATA TGTCATGCTT 
AGTGGGGTCT CCCCCTTCTT AGATGAGAGC AAAGAGGAAA CCTGTATCAA CGTGTGCAGG 
GTGGACTTTA GCTTCCCCCA CGAGTACTTC TGCGGTGTGA GCAATGCTGC CAGGGATTTC 
ATCAACGTAA TCTTACAGGA AGACTTCCGG AGGCGGCCCA CAGCAGCCAC GTGCCTGCAG 
CATCCCTGGC TACAGCCCCA CAACGGCAGC TACTCCAAGA TCCCCTTGGA CACCTCCCGT 
CTGGCCTGCT TCATAGAACG CCGCAAGCAC CAGAATGATG TGCGGCCCAT TCCCAATGTC 
AAGAGTTACA TCGTCAATAG GGTGAACCAA GGGACCTCGC TATCCCACAA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245952.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244254.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X2, mRNA.

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CloneID	OMu75308
Clone ID Related Accession (Same CDS sequence)	XM_011245952.3 , XM_011245952.2
Accession Version	XM_011245952.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGTCGTCTT TGCTAGCAGC CCGGCAGGCT CCCCCTGACG TGCCTACTGC TGCAGACCTT 
GTCAGTGCAA TAGAAAAGTT GGTCAAAAAT AAGCTGACTC TGGAAGGAGG CTCTTACCGG 
GGGAGCTTGA AGGACCCTAC AGGCTGCCTG AATGAGGGGA TGACCCCTCC CACACCTCCT 
AGAAACCTGG AAGAAGAACA GAAAGCCAAG GCGCTGAGAG GCAGGATGTT TGTCCTGAAT 
GAGCTGGTAC AGACAGAGAA GGACTACGTC AAGGACCTGG GGATTGTGGT GGAGGGCTTC 
ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT GAGGACATGC GAGGGAAGGA AAAGATCGTG 
TTTGGAAATA TCCACCAGAT TTACGACTGG CATAAAGATT TTTTCCTGGC TGAACTGGAA 
AAATGTATCC AAGAGCAAGA CAGATTGGCC CAGCTCTTCA TTAAACACGA GCGGAAGCTG 
CACATCTACG TGTGGTACTG CCAGAACAAG CCGCGTTCCG AGTACATCGT GGCTGAGTAC 
GATGCCTATT TTGAGGAGGT TAAACAAGAG ATCAATCAAA GGCTGACCCT TAGTGACTTC 
CTGATCAAGC CCATTCAGAG AATAACAAAG TACCAGCTGC TACTCAAGGA CTTCCTGCGA 
TACAGCGAGA AGGCTGGTTT GGAGTGTTCC GATATTGAGA AAGCAGTGGA GTTAATGTGC 
CTTGTCCCCA AGCGCTGTAA TGACATGATG AATCTGGGAC GCCTGCAGGG CTTCGAGGGC 
ACCCTGACCG CCCAGGGCAA GCTACTGCAG CAGGACACAT TCTATGTGAT CGAGCTGGAT 
GCCGGCATGC AGTCCCGGAC CAAGGAGAGG CGTGTGTTTC TCTTTGAGCA GATTGTCATC 
TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC ACCCCAGGCT ACATGTTCAA AAGAAGCATC 
AAGATGAATT ACTTGGTCCT GGAAGACAAT GTGGATGGTG ACCCCTGCAA ATTTGCACTC 
ATGAACAGGG AGACTTCAGA AAGGGTCATT CTACAGGCTG CCAACTCAGA CATCCAGCAG 
GCCTGGGTGC AGGACATCAA CCAAGTCCTA GAAACACAGA GGGACTTCTT AAATGCACTA 
CAGTCACCCA TTGAGTATCA GCGGAAGGAG AGAAGCACAG CTGTGATCCG GTCCCAGCCC 
CCGAGGGTTC CACAAGCCAG CCCCAGGCCC TACTCTTCTG GCCCGGTGGG CTCAGAGAAG 
CCCCCCAAGG GCTCCAGCTA TAACCCGCCT CTGCCACCCC TGAAGATATC TACCTCTAAT 
GGCAGTCCAG GGTTCGACTA CCATCAGCCT GGGGACAAGT TTGACGCTAG CAAGCAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AGAAGTCATG CTCTTGGCAC ACTCTACGCA TGAGAAAGCG GGCAGACGTG 
GAGAACTCGG GTAAAAATGA AGCCACAGGG CCTCGTAAAC CCAAGGATAT TCTGGGCAAC 
AAAGTCTCTG TTAAAGAGAC GAACAGTTCC GAGGAGTCAG AGTGTGATGA TCTTGACCCT 
AATACTAGTA TGGAGATCTT GAATCCAAAT TTCATCCAGG AAGTGGCTCC AGAATTCCTC 
GTGCCCTTAG TAGATGTGAC CTGTTTGCTT GGAGACACAG TGCTACTGCA GTGTAAAGCC 
TGTGGGCGGC CAAAGCCCAG CATCACCTGG AAGGGTCCGG ATCAGAACAT CCTAGACACT 
GACAACAGTT CAGCAACATA CACCATCTCC TCCTGTGATT CTGGGGAAAG CACCCTCAAG 
ATCTGCAACC TGATGCCCCA GGATAGTGGG ATTTATACCT GCATAGCAGC AAACGACCAC 
GGGACTGCAT CCACATCTGC TACAGTTAAG GTGCAAGGTG TCCCCGCAGC CCCCAACCGC 
CCAATCGCAC AGGAGAGGAG CTGCACCTCC GTCATCCTCC GCTGGCTGCC CCCAGCCAGC 
ACCGGAAACT GCACTATTTC GGGGTACACA GTGGAGTATC GCGAGGAAGG TTCCCAGGTC 
TGGCAGCAAT CCGTAGCTTC GACCTTGGAC ACTTACCTGG TCATTGAAGA CCTTAGTCCT 
GGGTGCCCTT ATCAGTTCAG GGTCAGTGCC AGCAACCCCT GGGGAATCAG CCTTCCCAGT 
GAGCCCTCGG AGTTTGTGCG CCTTCCAGAG TACGATGCAG CCGCCGACGG TGCCACCATT 
TCTTGGAAGG AAAATTTTGA TTCTGCTTAC ACTGAACTGA ACGAGATTGG CAGAGGCCGT 
TTCTCTATTG TAAAGAAATG CATTCACAAA GCTACCCGCA AAGATGTTGC TGTGAAATTT 
GTGAGCAAAA AAATGAAGAA GAAGGAGCAG GCTGCTCACG AGGCCGCCCT GCTCCAGCAC 
CTGCAGCACC CTCAGTATGT CACGCTCCAT GACACCTACG AGTCCCCCAC ATCCTACATC 
CTGATTTTGG AACTGATGGA TGACGGCCGA CTCTTGGACT ACCTTATGAA TCATGATGAG 
CTGATGGAAG AAAAGGTAGC TTTCTACATT CGAGACATCA TGGAAGCTCT GCAGTACCTG 
CACAACTGTA GGGTCGCACA TTTGGACATA AAGCCCGAAA ACCTGCTCAT TGACCTCCGG 
ATCCCAGTGC CTCGAGTGAA GCTCATCGAC TTGGAAGACG CTGTCCAGAT CTCAGGCCAC 
TTCCATATCC ACCATCTGCT GGGGAACCCT GAGTTCGCTG CCCCCGAAGT GATCCAAGGC 
ATCCCTGTCT CCCTGGGCAC AGACATCTGG AGCATCGGGG TTCTGACATA TGTCATGCTT 
AGTGGGGTCT CCCCCTTCTT AGATGAGAGC AAAGAGGAAA CCTGTATCAA CGTGTGCAGG 
GTGGACTTTA GCTTCCCCCA CGAGTACTTC TGCGGTGTGA GCAATGCTGC CAGGGATTTC 
ATCAACGTAA TCTTACAGGA AGACTTCCGG AGGCGGCCCA CAGCAGCCAC GTGCCTGCAG 
CATCCCTGGC TACAGCCCCA CAACGGCAGC TACTCCAAGA TCCCCTTGGA CACCTCCCGT 
CTGGCCTGCT TCATAGAACG CCGCAAGCAC CAGAATGATG TGCGGCCCAT TCCCAATGTC 
AAGAGTTACA TCGTCAATAG GGTGAACCAA GGGACCTCGC TATCCCACAA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244254.1
CDS	4891..13887
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245952.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245952.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGTCGTCTT TGCTAGCAGC CCGGCAGGCT CCCCCTGACG TGCCTACTGC TGCAGACCTT 
GTCAGTGCAA TAGAAAAGTT GGTCAAAAAT AAGCTGACTC TGGAAGGAGG CTCTTACCGG 
GGGAGCTTGA AGGACCCTAC AGGCTGCCTG AATGAGGGGA TGACCCCTCC CACACCTCCT 
AGAAACCTGG AAGAAGAACA GAAAGCCAAG GCGCTGAGAG GCAGGATGTT TGTCCTGAAT 
GAGCTGGTAC AGACAGAGAA GGACTACGTC AAGGACCTGG GGATTGTGGT GGAGGGCTTC 
ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT GAGGACATGC GAGGGAAGGA AAAGATCGTG 
TTTGGAAATA TCCACCAGAT TTACGACTGG CATAAAGATT TTTTCCTGGC TGAACTGGAA 
AAATGTATCC AAGAGCAAGA CAGATTGGCC CAGCTCTTCA TTAAACACGA GCGGAAGCTG 
CACATCTACG TGTGGTACTG CCAGAACAAG CCGCGTTCCG AGTACATCGT GGCTGAGTAC 
GATGCCTATT TTGAGGAGGT TAAACAAGAG ATCAATCAAA GGCTGACCCT TAGTGACTTC 
CTGATCAAGC CCATTCAGAG AATAACAAAG TACCAGCTGC TACTCAAGGA CTTCCTGCGA 
TACAGCGAGA AGGCTGGTTT GGAGTGTTCC GATATTGAGA AAGCAGTGGA GTTAATGTGC 
CTTGTCCCCA AGCGCTGTAA TGACATGATG AATCTGGGAC GCCTGCAGGG CTTCGAGGGC 
ACCCTGACCG CCCAGGGCAA GCTACTGCAG CAGGACACAT TCTATGTGAT CGAGCTGGAT 
GCCGGCATGC AGTCCCGGAC CAAGGAGAGG CGTGTGTTTC TCTTTGAGCA GATTGTCATC 
TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC ACCCCAGGCT ACATGTTCAA AAGAAGCATC 
AAGATGAATT ACTTGGTCCT GGAAGACAAT GTGGATGGTG ACCCCTGCAA ATTTGCACTC 
ATGAACAGGG AGACTTCAGA AAGGGTCATT CTACAGGCTG CCAACTCAGA CATCCAGCAG 
GCCTGGGTGC AGGACATCAA CCAAGTCCTA GAAACACAGA GGGACTTCTT AAATGCACTA 
CAGTCACCCA TTGAGTATCA GCGGAAGGAG AGAAGCACAG CTGTGATCCG GTCCCAGCCC 
CCGAGGGTTC CACAAGCCAG CCCCAGGCCC TACTCTTCTG GCCCGGTGGG CTCAGAGAAG 
CCCCCCAAGG GCTCCAGCTA TAACCCGCCT CTGCCACCCC TGAAGATATC TACCTCTAAT 
GGCAGTCCAG GGTTCGACTA CCATCAGCCT GGGGACAAGT TTGACGCTAG CAAGCAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AGAAGTCATG CTCTTGGCAC ACTCTACGCA TGAGAAAGCG GGCAGACGTG 
GAGAACTCGG GTAAAAATGA AGCCACAGGG CCTCGTAAAC CCAAGGATAT TCTGGGCAAC 
AAAGTCTCTG TTAAAGAGAC GAACAGTTCC GAGGAGTCAG AGTGTGATGA TCTTGACCCT 
AATACTAGTA TGGAGATCTT GAATCCAAAT TTCATCCAGG AAGTGGCTCC AGAATTCCTC 
GTGCCCTTAG TAGATGTGAC CTGTTTGCTT GGAGACACAG TGCTACTGCA GTGTAAAGCC 
TGTGGGCGGC CAAAGCCCAG CATCACCTGG AAGGGTCCGG ATCAGAACAT CCTAGACACT 
GACAACAGTT CAGCAACATA CACCATCTCC TCCTGTGATT CTGGGGAAAG CACCCTCAAG 
ATCTGCAACC TGATGCCCCA GGATAGTGGG ATTTATACCT GCATAGCAGC AAACGACCAC 
GGGACTGCAT CCACATCTGC TACAGTTAAG GTGCAAGGTG TCCCCGCAGC CCCCAACCGC 
CCAATCGCAC AGGAGAGGAG CTGCACCTCC GTCATCCTCC GCTGGCTGCC CCCAGCCAGC 
ACCGGAAACT GCACTATTTC GGGGTACACA GTGGAGTATC GCGAGGAAGG TTCCCAGGTC 
TGGCAGCAAT CCGTAGCTTC GACCTTGGAC ACTTACCTGG TCATTGAAGA CCTTAGTCCT 
GGGTGCCCTT ATCAGTTCAG GGTCAGTGCC AGCAACCCCT GGGGAATCAG CCTTCCCAGT 
GAGCCCTCGG AGTTTGTGCG CCTTCCAGAG TACGATGCAG CCGCCGACGG TGCCACCATT 
TCTTGGAAGG AAAATTTTGA TTCTGCTTAC ACTGAACTGA ACGAGATTGG CAGAGGCCGT 
TTCTCTATTG TAAAGAAATG CATTCACAAA GCTACCCGCA AAGATGTTGC TGTGAAATTT 
GTGAGCAAAA AAATGAAGAA GAAGGAGCAG GCTGCTCACG AGGCCGCCCT GCTCCAGCAC 
CTGCAGCACC CTCAGTATGT CACGCTCCAT GACACCTACG AGTCCCCCAC ATCCTACATC 
CTGATTTTGG AACTGATGGA TGACGGCCGA CTCTTGGACT ACCTTATGAA TCATGATGAG 
CTGATGGAAG AAAAGGTAGC TTTCTACATT CGAGACATCA TGGAAGCTCT GCAGTACCTG 
CACAACTGTA GGGTCGCACA TTTGGACATA AAGCCCGAAA ACCTGCTCAT TGACCTCCGG 
ATCCCAGTGC CTCGAGTGAA GCTCATCGAC TTGGAAGACG CTGTCCAGAT CTCAGGCCAC 
TTCCATATCC ACCATCTGCT GGGGAACCCT GAGTTCGCTG CCCCCGAAGT GATCCAAGGC 
ATCCCTGTCT CCCTGGGCAC AGACATCTGG AGCATCGGGG TTCTGACATA TGTCATGCTT 
AGTGGGGTCT CCCCCTTCTT AGATGAGAGC AAAGAGGAAA CCTGTATCAA CGTGTGCAGG 
GTGGACTTTA GCTTCCCCCA CGAGTACTTC TGCGGTGTGA GCAATGCTGC CAGGGATTTC 
ATCAACGTAA TCTTACAGGA AGACTTCCGG AGGCGGCCCA CAGCAGCCAC GTGCCTGCAG 
CATCCCTGGC TACAGCCCCA CAACGGCAGC TACTCCAAGA TCCCCTTGGA CACCTCCCGT 
CTGGCCTGCT TCATAGAACG CCGCAAGCAC CAGAATGATG TGCGGCCCAT TCCCAATGTC 
AAGAGTTACA TCGTCAATAG GGTGAACCAA GGGACCTCGC TATCCCACAA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245953.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244255.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X3, mRNA.

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CloneID	OMu75309
Clone ID Related Accession (Same CDS sequence)	XM_011245953.3 , XM_011245953.2
Accession Version	XM_011245953.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245953.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AGAAGTCATG CTCTTGGCAC ACTCTACGCA TGAGAAAGCG GGCAGACGTG 
GAGAACTCGG GTAAAAATGA AGCCACAGGG CCTCGTAAAC CCAAGGATAT TCTGGGCAAC 
AAAGTCTCTG TTAAAGAGAC GAACAGTTCC GAGGAGTCAG AGTGTGATGA TCTTGACCCT 
AATACTAGTA TGGAGATCTT GAATCCAAAT TTCATCCAGG AAGTGGCTCC AGAATTCCTC 
GTGCCCTTAG TAGATGTGAC CTGTTTGCTT GGAGACACAG TGCTACTGCA GTGTAAAGCC 
TGTGGGCGGC CAAAGCCCAG CATCACCTGG AAGGGTCCGG ATCAGAACAT CCTAGACACT 
GACAACAGTT CAGCAACATA CACCATCTCC TCCTGTGATT CTGGGGAAAG CACCCTCAAG 
ATCTGCAACC TGATGCCCCA GGATAGTGGG ATTTATACCT GCATAGCAGC AAACGACCAC 
GGGACTGCAT CCACATCTGC TACAGTTAAG GTGCAAGGTG TCCCCGCAGC CCCCAACCGC 
CCAATCGCAC AGGAGAGGAG CTGCACCTCC GTCATCCTCC GCTGGCTGCC CCCAGCCAGC 
ACCGGAAACT GCACTATTTC GGGGTACACA GTGGAGTATC GCGAGGAAGG TTCCCAGGTC 
TGGCAGCAAT CCGTAGCTTC GACCTTGGAC ACTTACCTGG TCATTGAAGA CCTTAGTCCT 
GGGTGCCCTT ATCAGTTCAG GGTCAGTGCC AGCAACCCCT GGGGAATCAG CCTTCCCAGT 
GAGCCCTCGG AGTTTGTGCG CCTTCCAGAG TACGATGCAG CCGCCGACGG TGCCACCATT 
TCTTGGAAGG AAAATTTTGA TTCTGCTTAC ACTGAACTGA ACGAGATTGG CAGAGGCCGT 
TTCTCTATTG TAAAGAAATG CATTCACAAA GCTACCCGCA AAGATGTTGC TGTGAAATTT 
GTGAGCAAAA AAATGAAGAA GAAGGAGCAG GCTGCTCACG AGGCCGCCCT GCTCCAGCAC 
CTGCAGCACC CTCAGTATGT CACGCTCCAT GACACCTACG AGTCCCCCAC ATCCTACATC 
CTGATTTTGG AACTGATGGA TGACGGCCGA CTCTTGGACT ACCTTATGAA TCATGATGAG 
CTGATGGAAG AAAAGGTAGC TTTCTACATT CGAGACATCA TGGAAGCTCT GCAGTACCTG 
CACAACTGTA GGGTCGCACA TTTGGACATA AAGCCCGAAA ACCTGCTCAT TGACCTCCGG 
ATCCCAGTGC CTCGAGTGAA GCTCATCGAC TTGGAAGACG CTGTCCAGAT CTCAGGCCAC 
TTCCATATCC ACCATCTGCT GGGGAACCCT GAGTTCGCTG CCCCCGAAGT GATCCAAGGC 
ATCCCTGTCT CCCTGGGCAC AGACATCTGG AGCATCGGGG TTCTGACATA TGTCATGCTT 
AGTGGGGTCT CCCCCTTCTT AGATGAGAGC AAAGAGGAAA CCTGTATCAA CGTGTGCAGG 
GTGGACTTTA GCTTCCCCCA CGAGTACTTC TGCGGTGTGA GCAATGCTGC CAGGGATTTC 
ATCAACGTAA TCTTACAGGA AGACTTCCGG AGGCGGCCCA CAGCAGCCAC GTGCCTGCAG 
CATCCCTGGC TACAGCCCCA CAACGGCAGC TACTCCAAGA TCCCCTTGGA CACCTCCCGT 
CTGGCCTGCT TCATAGAACG CCGCAAGCAC CAGAATGATG TGCGGCCCAT TCCCAATGTC 
AAGAGTTACA TCGTCAATAG GGTGAACCAA GGGACCTCGC TATCCCACAA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244255.1
CDS	24875..33871
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245953.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245953.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AGAAGTCATG CTCTTGGCAC ACTCTACGCA TGAGAAAGCG GGCAGACGTG 
GAGAACTCGG GTAAAAATGA AGCCACAGGG CCTCGTAAAC CCAAGGATAT TCTGGGCAAC 
AAAGTCTCTG TTAAAGAGAC GAACAGTTCC GAGGAGTCAG AGTGTGATGA TCTTGACCCT 
AATACTAGTA TGGAGATCTT GAATCCAAAT TTCATCCAGG AAGTGGCTCC AGAATTCCTC 
GTGCCCTTAG TAGATGTGAC CTGTTTGCTT GGAGACACAG TGCTACTGCA GTGTAAAGCC 
TGTGGGCGGC CAAAGCCCAG CATCACCTGG AAGGGTCCGG ATCAGAACAT CCTAGACACT 
GACAACAGTT CAGCAACATA CACCATCTCC TCCTGTGATT CTGGGGAAAG CACCCTCAAG 
ATCTGCAACC TGATGCCCCA GGATAGTGGG ATTTATACCT GCATAGCAGC AAACGACCAC 
GGGACTGCAT CCACATCTGC TACAGTTAAG GTGCAAGGTG TCCCCGCAGC CCCCAACCGC 
CCAATCGCAC AGGAGAGGAG CTGCACCTCC GTCATCCTCC GCTGGCTGCC CCCAGCCAGC 
ACCGGAAACT GCACTATTTC GGGGTACACA GTGGAGTATC GCGAGGAAGG TTCCCAGGTC 
TGGCAGCAAT CCGTAGCTTC GACCTTGGAC ACTTACCTGG TCATTGAAGA CCTTAGTCCT 
GGGTGCCCTT ATCAGTTCAG GGTCAGTGCC AGCAACCCCT GGGGAATCAG CCTTCCCAGT 
GAGCCCTCGG AGTTTGTGCG CCTTCCAGAG TACGATGCAG CCGCCGACGG TGCCACCATT 
TCTTGGAAGG AAAATTTTGA TTCTGCTTAC ACTGAACTGA ACGAGATTGG CAGAGGCCGT 
TTCTCTATTG TAAAGAAATG CATTCACAAA GCTACCCGCA AAGATGTTGC TGTGAAATTT 
GTGAGCAAAA AAATGAAGAA GAAGGAGCAG GCTGCTCACG AGGCCGCCCT GCTCCAGCAC 
CTGCAGCACC CTCAGTATGT CACGCTCCAT GACACCTACG AGTCCCCCAC ATCCTACATC 
CTGATTTTGG AACTGATGGA TGACGGCCGA CTCTTGGACT ACCTTATGAA TCATGATGAG 
CTGATGGAAG AAAAGGTAGC TTTCTACATT CGAGACATCA TGGAAGCTCT GCAGTACCTG 
CACAACTGTA GGGTCGCACA TTTGGACATA AAGCCCGAAA ACCTGCTCAT TGACCTCCGG 
ATCCCAGTGC CTCGAGTGAA GCTCATCGAC TTGGAAGACG CTGTCCAGAT CTCAGGCCAC 
TTCCATATCC ACCATCTGCT GGGGAACCCT GAGTTCGCTG CCCCCGAAGT GATCCAAGGC 
ATCCCTGTCT CCCTGGGCAC AGACATCTGG AGCATCGGGG TTCTGACATA TGTCATGCTT 
AGTGGGGTCT CCCCCTTCTT AGATGAGAGC AAAGAGGAAA CCTGTATCAA CGTGTGCAGG 
GTGGACTTTA GCTTCCCCCA CGAGTACTTC TGCGGTGTGA GCAATGCTGC CAGGGATTTC 
ATCAACGTAA TCTTACAGGA AGACTTCCGG AGGCGGCCCA CAGCAGCCAC GTGCCTGCAG 
CATCCCTGGC TACAGCCCCA CAACGGCAGC TACTCCAAGA TCCCCTTGGA CACCTCCCGT 
CTGGCCTGCT TCATAGAACG CCGCAAGCAC CAGAATGATG TGCGGCCCAT TCCCAATGTC 
AAGAGTTACA TCGTCAATAG GGTGAACCAA GGGACCTCGC TATCCCACAA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245953.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244255.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X3, mRNA.

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CloneID	OMu75309
Clone ID Related Accession (Same CDS sequence)	XM_011245953.3 , XM_011245953.2
Accession Version	XM_011245953.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AGAAGTCATG CTCTTGGCAC ACTCTACGCA TGAGAAAGCG GGCAGACGTG 
GAGAACTCGG GTAAAAATGA AGCCACAGGG CCTCGTAAAC CCAAGGATAT TCTGGGCAAC 
AAAGTCTCTG TTAAAGAGAC GAACAGTTCC GAGGAGTCAG AGTGTGATGA TCTTGACCCT 
AATACTAGTA TGGAGATCTT GAATCCAAAT TTCATCCAGG AAGTGGCTCC AGAATTCCTC 
GTGCCCTTAG TAGATGTGAC CTGTTTGCTT GGAGACACAG TGCTACTGCA GTGTAAAGCC 
TGTGGGCGGC CAAAGCCCAG CATCACCTGG AAGGGTCCGG ATCAGAACAT CCTAGACACT 
GACAACAGTT CAGCAACATA CACCATCTCC TCCTGTGATT CTGGGGAAAG CACCCTCAAG 
ATCTGCAACC TGATGCCCCA GGATAGTGGG ATTTATACCT GCATAGCAGC AAACGACCAC 
GGGACTGCAT CCACATCTGC TACAGTTAAG GTGCAAGGTG TCCCCGCAGC CCCCAACCGC 
CCAATCGCAC AGGAGAGGAG CTGCACCTCC GTCATCCTCC GCTGGCTGCC CCCAGCCAGC 
ACCGGAAACT GCACTATTTC GGGGTACACA GTGGAGTATC GCGAGGAAGG TTCCCAGGTC 
TGGCAGCAAT CCGTAGCTTC GACCTTGGAC ACTTACCTGG TCATTGAAGA CCTTAGTCCT 
GGGTGCCCTT ATCAGTTCAG GGTCAGTGCC AGCAACCCCT GGGGAATCAG CCTTCCCAGT 
GAGCCCTCGG AGTTTGTGCG CCTTCCAGAG TACGATGCAG CCGCCGACGG TGCCACCATT 
TCTTGGAAGG AAAATTTTGA TTCTGCTTAC ACTGAACTGA ACGAGATTGG CAGAGGCCGT 
TTCTCTATTG TAAAGAAATG CATTCACAAA GCTACCCGCA AAGATGTTGC TGTGAAATTT 
GTGAGCAAAA AAATGAAGAA GAAGGAGCAG GCTGCTCACG AGGCCGCCCT GCTCCAGCAC 
CTGCAGCACC CTCAGTATGT CACGCTCCAT GACACCTACG AGTCCCCCAC ATCCTACATC 
CTGATTTTGG AACTGATGGA TGACGGCCGA CTCTTGGACT ACCTTATGAA TCATGATGAG 
CTGATGGAAG AAAAGGTAGC TTTCTACATT CGAGACATCA TGGAAGCTCT GCAGTACCTG 
CACAACTGTA GGGTCGCACA TTTGGACATA AAGCCCGAAA ACCTGCTCAT TGACCTCCGG 
ATCCCAGTGC CTCGAGTGAA GCTCATCGAC TTGGAAGACG CTGTCCAGAT CTCAGGCCAC 
TTCCATATCC ACCATCTGCT GGGGAACCCT GAGTTCGCTG CCCCCGAAGT GATCCAAGGC 
ATCCCTGTCT CCCTGGGCAC AGACATCTGG AGCATCGGGG TTCTGACATA TGTCATGCTT 
AGTGGGGTCT CCCCCTTCTT AGATGAGAGC AAAGAGGAAA CCTGTATCAA CGTGTGCAGG 
GTGGACTTTA GCTTCCCCCA CGAGTACTTC TGCGGTGTGA GCAATGCTGC CAGGGATTTC 
ATCAACGTAA TCTTACAGGA AGACTTCCGG AGGCGGCCCA CAGCAGCCAC GTGCCTGCAG 
CATCCCTGGC TACAGCCCCA CAACGGCAGC TACTCCAAGA TCCCCTTGGA CACCTCCCGT 
CTGGCCTGCT TCATAGAACG CCGCAAGCAC CAGAATGATG TGCGGCCCAT TCCCAATGTC 
AAGAGTTACA TCGTCAATAG GGTGAACCAA GGGACCTCGC TATCCCACAA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244255.1
CDS	4891..13887
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245953.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245953.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGAAC 
GACCTGGGAG GCTGCAATGG GACATCAACC ATGACTGTAA TCAAAGATTA CTATGCACTG 
AAGGAGAATG AAATCTGCGT GAGCCAAGGT GAGGTGGTAC AGGTCCTCGC CGTCAACCAG 
CAGAACATGT GTCTGGTGTA CCAGCCTGCC AGCGACCATT CCCCGGCAGC AGAGGGCTGG 
GTCCCAGGCA GCATCCTGGC GCCCCTCGCC AAAGCCACAG CAGCGGCCGA AAGTAGTGAC 
GGGAGCATCA AGAAGTCATG CTCTTGGCAC ACTCTACGCA TGAGAAAGCG GGCAGACGTG 
GAGAACTCGG GTAAAAATGA AGCCACAGGG CCTCGTAAAC CCAAGGATAT TCTGGGCAAC 
AAAGTCTCTG TTAAAGAGAC GAACAGTTCC GAGGAGTCAG AGTGTGATGA TCTTGACCCT 
AATACTAGTA TGGAGATCTT GAATCCAAAT TTCATCCAGG AAGTGGCTCC AGAATTCCTC 
GTGCCCTTAG TAGATGTGAC CTGTTTGCTT GGAGACACAG TGCTACTGCA GTGTAAAGCC 
TGTGGGCGGC CAAAGCCCAG CATCACCTGG AAGGGTCCGG ATCAGAACAT CCTAGACACT 
GACAACAGTT CAGCAACATA CACCATCTCC TCCTGTGATT CTGGGGAAAG CACCCTCAAG 
ATCTGCAACC TGATGCCCCA GGATAGTGGG ATTTATACCT GCATAGCAGC AAACGACCAC 
GGGACTGCAT CCACATCTGC TACAGTTAAG GTGCAAGGTG TCCCCGCAGC CCCCAACCGC 
CCAATCGCAC AGGAGAGGAG CTGCACCTCC GTCATCCTCC GCTGGCTGCC CCCAGCCAGC 
ACCGGAAACT GCACTATTTC GGGGTACACA GTGGAGTATC GCGAGGAAGG TTCCCAGGTC 
TGGCAGCAAT CCGTAGCTTC GACCTTGGAC ACTTACCTGG TCATTGAAGA CCTTAGTCCT 
GGGTGCCCTT ATCAGTTCAG GGTCAGTGCC AGCAACCCCT GGGGAATCAG CCTTCCCAGT 
GAGCCCTCGG AGTTTGTGCG CCTTCCAGAG TACGATGCAG CCGCCGACGG TGCCACCATT 
TCTTGGAAGG AAAATTTTGA TTCTGCTTAC ACTGAACTGA ACGAGATTGG CAGAGGCCGT 
TTCTCTATTG TAAAGAAATG CATTCACAAA GCTACCCGCA AAGATGTTGC TGTGAAATTT 
GTGAGCAAAA AAATGAAGAA GAAGGAGCAG GCTGCTCACG AGGCCGCCCT GCTCCAGCAC 
CTGCAGCACC CTCAGTATGT CACGCTCCAT GACACCTACG AGTCCCCCAC ATCCTACATC 
CTGATTTTGG AACTGATGGA TGACGGCCGA CTCTTGGACT ACCTTATGAA TCATGATGAG 
CTGATGGAAG AAAAGGTAGC TTTCTACATT CGAGACATCA TGGAAGCTCT GCAGTACCTG 
CACAACTGTA GGGTCGCACA TTTGGACATA AAGCCCGAAA ACCTGCTCAT TGACCTCCGG 
ATCCCAGTGC CTCGAGTGAA GCTCATCGAC TTGGAAGACG CTGTCCAGAT CTCAGGCCAC 
TTCCATATCC ACCATCTGCT GGGGAACCCT GAGTTCGCTG CCCCCGAAGT GATCCAAGGC 
ATCCCTGTCT CCCTGGGCAC AGACATCTGG AGCATCGGGG TTCTGACATA TGTCATGCTT 
AGTGGGGTCT CCCCCTTCTT AGATGAGAGC AAAGAGGAAA CCTGTATCAA CGTGTGCAGG 
GTGGACTTTA GCTTCCCCCA CGAGTACTTC TGCGGTGTGA GCAATGCTGC CAGGGATTTC 
ATCAACGTAA TCTTACAGGA AGACTTCCGG AGGCGGCCCA CAGCAGCCAC GTGCCTGCAG 
CATCCCTGGC TACAGCCCCA CAACGGCAGC TACTCCAAGA TCCCCTTGGA CACCTCCCGT 
CTGGCCTGCT TCATAGAACG CCGCAAGCAC CAGAATGATG TGCGGCCCAT TCCCAATGTC 
AAGAGTTACA TCGTCAATAG GGTGAACCAA GGGACCTCGC TATCCCACAA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245954.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244256.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X4, mRNA.

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CloneID	OMu75310
Clone ID Related Accession (Same CDS sequence)	XM_011245954.2 , XM_011245954.3
Accession Version	XM_011245954.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGTA TCCTGAGATC TTTTCGGAAT GATGGTTTGA AAGCTTCTGA TGTCCTTCCT 
ATCCTAAAGG AGAAAGTGGC CTTTGTGTCT GGGGGGCGTG ACAAGCGAGG TGGACCCATC 
CTGACCTTCC CTGCCCGAAG CAATCATGAC AGAATAAGAC AGGAAGACCT ACGGAAACTT 
GTGACGTATT TGGCCAGCGT GCCAAGTGAG GACGTTTGCA AACGTGGCTT CACCGTCATC 
ATCGACATGC GGGGCTCCAA GTGGGATCTC ATCAAGCCCC TCCTCAAGAC GCTGCAGGAA 
GCCTTCCCCG CCGAGATCCA TGTGGCCCTC ATCATCAAGC CTGACAACTT CTGGCAGAAG 
CAGAAGACCA ACTTTGGCAG CTCCAAATTC ATCTTTGAGA CAAGCATGGT GTCTGTGGAG 
GGCCTCACGA AGCTGGTGGA CCCCTCCCAG CTGACAGAGG AGTTTGACGG CTCCCTGGAT 
TACAACCATG AGGAGTGGAT TGAGTTGCGC CTCTCCCTGG AGGAGTTCTT CAACAGTGCG 
GTCCACCTGC TCTCACGCCT TGAGGACCTG CAGGAGATGC TGGCCCGGAA GGAGTTCCCT 
GTGGATGTGG AGGGCTCTCG GAGGCTTATC GACGAGCACA CGCAACTTAA GAAAAAGGTG 
CTGAAGGCCC CCGTGGAAGA GCTGGACCGG GAGGGGCAGC GTCTACTGCA GTGCATCCGC 
TGCAGCGATG GCTTCTCAGG GCGCAACTGC ATCCCAGGAA GTGCCGACTT CCAGAGCCTT 
GTACCCAAGA TCACCAGCCT CCTGGACAAG CTGCATTCCA CCCGACAACA CCTGCACCAA 
ATGTGGCACG TGCGAAAGCT CAAGCTGGAT CAGTGCTTCC AGCTGCGGCT CTTCGAGCAG 
GATGCTGAGA AGATGTTCGA CTGGATAAGC CACAACAAGG AGTTATTTCT TCAGAGCCAC 
ACAGAGATCG GAGTCAGCTA CCAGCATGCA CTAGACCTCC AGACACAGCA CAATCACTTT 
GCCATGAACT CCATGAATGC CTATGTCAAC ATCAACCGCA TCATGTCTGT CGCTTCCCGC 
CTCTCAGAGG CCGGCCATTA TGCCTCCCAG CAAATCAAGC AGATCTCCAC GCAGCTAGAC 
CAGGAGTGGA AGAGCTTTGC CGCTGCCCTG GATGAACGCA GTACAATCCT GGCCATGTCT 
GCTGTGTTCC ACCAGAAGGC AGAGCAGTTC CTGTCAGGCG TGGATGCCTG GTGTAAGATG 
TGCAGCGAAG GCGGCCTTCC TTCTGAGATG CAGGACCTGG AGCTGGCAAT CCACCATCAC 
CAGTCCTTAT ATGAGCAGGT GACCCAGGCC TACACAGAGG TCAGCCAGGA TGGCAAAGCA 
CTACTGGATG TCCTGCAGCG TCCCCTGAGT CCTGGGAACT CTGAGTCCCT CACAGCCACA 
GCCAACTACT CCAAGGCGGT CCACCAGGTG CTGGATGTGG TCCACGAGGT GCTGCACCAT 
CAGCGACGGC TCGAGAGCAT CTGGCAGCAC CGCAAGGTGC GGCTTCACCA GCGGCTGCAG 
CTGTGTGTCT TCCAGCAGGA TGTGCAGCAG GTGCTGGACT GGATTGAAAA CCATGGCGAG 
GCCTTTCTCA GCAAACACAC TGGGGTTGGC AAGTCCTTGC ATCGAGCACG TGCACTACAG 
AAGAGGCATG ATGACTTTGA AGAAGTGGCA CAGAATACAT ACACCAATGC GGACAAGCTC 
CTAGAAGCAG CGGAGCAGCT GGCTCAGACT GGGGAATGTG ACCCGGAGGA GATCTACAAG 
GCAGCCCGGC ACCTGGAGGT GCGCATCCAA GACTTTGTGC GCAGAGTGGA GCAGCGGAAG 
CTGCTTCTGG ACATGTCGGT CTCTTTCCAC ACGCACACCA AAGAGCTGTG GACATGGATG 
GAGGACCTTC AGAAGGAGGT GCTGGAGGAT GTCTGTGCAG ACTCTGTGGA TGCGGTCCAG 
GAGCTGATCA AGCAGTTCCA GCAGCAGCAG ACCGCCACCC TGGATGCCAC TCTCAATGTC 
ATCAAGGAAG GCGAAGACCT TATCCAGCAG CTCAGGTCAG CGCCTCCCTC CCTGGGGGAG 
CCCACCGAGG CCAGGGACTC AGCGATGTCC AACAACAAGA CACCCCACAG TAGCTCTATC 
AGCCATATTG AGTCTGTCCT CCAGCAGCTG GACGATGCTC AGGTACAGAT GGAAGAGCTG 
TTTCACGAGC GGAAGATCAA GCTGGACATC TTCCTACAGC TGCGCATCTT TGAACAGTAC 
ACCATTGAGG TCACTGCAGA GCTGGATGCC TGGAACGAGG ACTTGCTCCG GCAGATGAAT 
GATTTCAACA CAGAGGATCT GACCCTGGCT GAGCAGCGGC TACAGCGCCA CACAGAACGG 
AAGCTTGCCA TGAACAACAT GACGTTCGAG GTCATCCAGC AGGGCCAGGA CCTGCACCAG 
TACATCATGG AAGTGCAAGC GTCAGGAATC GAGTTAATCT GTGAGAAGGA CCTTGACCTG 
GCAGCTCAGG TGCAGGAGCT GCTGGAGTTT CTCCATGAGA AGCAGCACGA GCTGGAGCTC 
AATGCGGAGC AGACGCACAA GCGGCTAGAG CAGTGTCTCC AGCTGAGGCA CCTCCAGGCT 
GAGGTCAAAC AGGTTCTGGG ATGGATCCGC AATGGAGAGT CGATGCTCAA CGCCAGCCTG 
GTCAACGCCA GCTCTTTGTC GGAGGCAGAG CAGCTGCAGC GGGAGCATGA ACAGTTCCAG 
CTAGCCATCG AGTCCCTCTT TCATGCCACT TCCTTGCAGA AGACGCACCA GAGCGCCCTG 
CAGGTACAGC AGAAGGCAGA GGCACTGCTC CAGGCCGGCC ACTACGACGC AGACGCCATC 
CGGGAATGTG CTGAGAAGGT GGCCCTCCAC TGGCAACAGC TCATGCTAAA GATGGAGGAC 
CGGCTGAAGC TTGTCAACGC CTCTGTGGCC TTTTACAAGA CCTCTGAGCA GGTGTGCAGT 
GTTCTGGAGA GCTTGGAGCA GGAGTACCGG AGAGACGAGG ACTGGTGTGG TGGGAGAGAC 
AAGCTGGGGC CGGCGGCAGA GATGGACCAC GTTATTCCAC TCCTCAGCAA GCACCTGGAG 
CAGAAGGAGG CCTTCCTGAA GGCCTGCACG CTGGCAAGGC GGAACGCCGA GGTGTTCCTC 
AAATACATCC ACAGGAACAA TGTGAGCATG CCTAGTGTTG CCAGCCACAC CCGGGGGCCT 
GAACAGCAAG TAAAAGCCAT CTTGAGCGAG CTCTTGCAGA GGGAGAATCG AGTCCTGCAC 
TTCTGGACCT TGAAGAAGCG GCGGTTAGAC CAGTGTCAGC AATATGTGGT GTTTGAGCGC 
AGTGCTAAGC AGGCACTGGA CTGGATCCAA GAAACAGGAG AATATTACCT CTCAACACAT 
ACCTCCACCG GAGAGACCAC GGAGGAGACT CAGGAGCTGC TGAAAGAGTA TGGAGAATTC 
AGGGTGCCTG CCAAGCAAAC GAAGGAGAAG GTGAAACTTC TGATCCAGCT AGCAGACAGC 
TTTGTGGAAA AGGGCCACAT CCACGCCACA GAGATACGGA AATGGGTGAC CACGGTGGAC 
AAGCACTACC GCGATTTCTC CCTGAGGATG GGGAAGTACC GGTACTCGCT GGAGAAGGCG 
CTGGGGGTCA ACACGGAGGA TAACAAGGAC CTAGAGCTGG ACATCATCCC AGCAAGCCTT 
TCGGATCGGG AGGTCAAGCT CCGGGACGCT AACCATGAGA TCAATGAAGA GAAACGGAAG 
TCAGCTCGAA AGAAAGAGTT CATTATGGCT GAACTGCTCC AAACTGAGAA GGCTTATGTA 
AGGGACTTGC ACGAGTGCTT GGAGACCTAC CTGTGGGAGA TGACTAGTGG TGTGGAAGAG 
ATACCCCCCG GCATCCTCAA TAAGGAACAT ATCATCTTCG GCAACATCCA GGAGATCTAT 
GACTTCCATA ACAACATCTT CCTCAAAGAG CTGGAGAAGT ACGAGCAGCT GCCTGAGGAT 
GTGGGACACT GCTTTGTTAC CTGGGCAGAC AAATTTCAGA TGTATGTCAC CTACTGTAAA 
AACAAGCCTG ATTCCAACCA GCTGATCCTG GAGCATGCGG GCACCTTCTT TGATGAGATC 
CAGCAGCGGC ATGGCCTGGC CAACTCCATC TCCTCCTACC TAATTAAGCC TGTCCAGAGG 
GTTACCAAGT ACCAGCTGCT CCTGAAGGAA CTTCTAACTT GCTGTGAAGA AGGGAAAGGA 
GAGCTGAAGG ATGGCCTGGA GGTGATGCTC AGTGTCCCCA AGAAAGCCAA CGATGCCATG 
CATGTCAGCA TGCTGGAAGG GTTCGATGAA AACTTGGATG TACAGGGGGA GTTGATTCTC 
CAGGATGCCT TTCAAGTGTG GGACCCAAAG TCGCTGATCC GGAAGGGGCG GGAGCGCCAT 
CTGTTCCTCT TTGAGATCTC CTTGGTGTTT AGCAAAGAGA TCAAAGACTC TTCAGGACAC 
ACGAAATATG TTTACAAGAA CAAGCTACTG ACCTCAGAGC TGGGTGTGAC CGAGCATGTA 
GAAGGTGACC CCTGCAAATT CGCCTTGTGG TCTGGGCGCA CCCCATCCTC AGACAATAAA 
ACAGTGCTCA AAGCTTCCAA CATCGAAACC AAGCAGGAAT GGATCAAGAA CATTCGAGAA 
GTGATTCAGG AGAGGATCAT TCATCTTAAA GGCGCTTTAA AGGAGCCAAT TCAGCTCCCC 
AAAACACCAG CCAAACTGAG GAACAATAGT AAGAGGGATG GGGTAGAGGA TGGTGACAGC 
CAAGGGGATG GGAGCAGCCA GCCAGATACC ATATCCATTG CCTCCAGGAC CTCTCAGAAC 
ACAGTGGAAA GTGACAAGCT CTCAGGTGGA TGTGAGCTGA CCGTGGTCCT CCAGGACTTC 
AGCGCAGGCC ACAGCAGCGA GCTGAGCATC CAGGTGGGCC AGACGGTGGA GCTGCTGGAA 
CGACCGAGTG AGAGGCCTGG CTGGTGTCTG GTCCGCACCA CAGAGCGGAG CCCCCCACAG 
GAAGGCCTGG TACCCAGCAG CGCCCTGTGC ATCTCACACT CTCGCAGCAG CGTGGAAATG 
GACTGCTTCT TCCCCTTGAA AGATTCATAC TCTCATTCCT CCAGTGAGAA TGGAGGCAAA 
TCCGAGTCCG TGGCCCACCT GCAGTCTCAG CCCTCCCTGA ACTCCATCCA CAGTTCCCCT 
GGTCCCAAGC GCTCCACCAA CACCCTCAAA AAGTGGCTGA CCAGCCCCGT GCGCCGCCTC 
AACAGCGGAA AAGCCGACGG GAACATCAAA AAGCAGAAGA AAGTCCGGGA TGGGCGGAAG 
AGCTTCGATC TGGGATCGCC CAAGCCTGGG GATGAGACGA CACCTCAGGG AGACAGCGCT 
GACGAGAAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GTCGTCTTTG 
CTAGCAGCCC GGCAGGCTCC CCCTGACGTG CCTACTGCTG CAGACCTTGT CAGTGCAATA 
GAAAAGTTGG TCAAAAATAA GCTGACTCTG GAAGGAGGCT CTTACCGGGG GAGCTTGAAG 
GACCCTACAG GCTGCCTGAA TGAGGGGATG ACCCCTCCCA CACCTCCTAG AAACCTGGAA 
GAAGAACAGA AAGCCAAGGC GCTGAGAGGC AGGATGTTTG TCCTGAATGA GCTGGTACAG 
ACAGAGAAGG ACTACGTCAA GGACCTGGGG ATTGTGGTGG AGGGCTTCAT GAAGAGAATA 
GAAGAGAAGG GGGTCCCTGA GGACATGCGA GGGAAGGAAA AGATCGTGTT TGGAAATATC 
CACCAGATTT ACGACTGGCA TAAAGATTTT TTCCTGGCTG AACTGGAAAA ATGTATCCAA 
GAGCAAGACA GATTGGCCCA GCTCTTCATT AAACACGAGC GGAAGCTGCA CATCTACGTG 
TGGTACTGCC AGAACAAGCC GCGTTCCGAG TACATCGTGG CTGAGTACGA TGCCTATTTT 
GAGGAGGTTA AACAAGAGAT CAATCAAAGG CTGACCCTTA GTGACTTCCT GATCAAGCCC 
ATTCAGAGAA TAACAAAGTA CCAGCTGCTA CTCAAGGACT TCCTGCGATA CAGCGAGAAG 
GCTGGTTTGG AGTGTTCCGA TATTGAGAAA GCAGTGGAGT TAATGTGCCT TGTCCCCAAG 
CGCTGTAATG ACATGATGAA TCTGGGACGC CTGCAGGGCT TCGAGGGCAC CCTGACCGCC 
CAGGGCAAGC TACTGCAGCA GGACACATTC TATGTGATCG AGCTGGATGC CGGCATGCAG 
TCCCGGACCA AGGAGAGGCG TGTGTTTCTC TTTGAGCAGA TTGTCATCTT CAGTGAACTG 
CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC ATGTTCAAAA GAAGCATCAA GATGAATTAC 
TTGGTCCTGG AAGACAATGT GGATGGTGAC CCCTGCAAAT TTGCACTCAT GAACAGGGAG 
ACTTCAGAAA GGGTCATTCT ACAGGCTGCC AACTCAGACA TCCAGCAGGC CTGGGTGCAG 
GACATCAACC AAGTCCTAGA AACACAGAGG GACTTCTTAA ATGCACTACA GTCACCCATT 
GAGTATCAGC GGAAGGAGAG AAGCACAGCT GTGATCCGGT CCCAGCCCCC GAGGGTTCCA 
CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC 
TCCAGCTATA ACCCGCCTCT GCCACCCCTG AAGATATCTA CCTCTAATGG CAGTCCAGGG 
TTCGACTACC ATCAGCCTGG GGACAAGTTT GACGCTAGCA AGCAGAACGA CCTGGGAGGC 
TGCAATGGGA CATCAACCAT GACTGTAATC AAAGATTACT ATGCACTGAA GGAGAATGAA 
ATCTGCGTGA GCCAAGGTGA GGTGGTACAG GTCCTCGCCG TCAACCAGCA GAACATGTGT 
CTGGTGTACC AGCCTGCCAG CGACCATTCC CCGGCAGCAG AGGGCTGGGT CCCAGGCAGC 
ATCCTGGCGC CCCTCGCCAA AGCCACAGCA GCGGCCGAAA GTAGTGACGG GAGCATCAAG 
AAGTCATGCT CTTGGCACAC TCTACGCATG AGAAAGCGGG CAGACGTGGA GAACTCGGGT 
AAAAATGAAG CCACAGGGCC TCGTAAACCC AAGGATATTC TGGGCAACAA AGTCTCTGTT 
AAAGAGACGA ACAGTTCCGA GGAGTCAGAG TGTGATGATC TTGACCCTAA TACTAGTATG 
GAGATCTTGA ATCCAAATTT CATCCAGGAA GTGGCTCCAG AATTCCTCGT GCCCTTAGTA 
GATGTGACCT GTTTGCTTGG AGACACAGTG CTACTGCAGT GTAAAGCCTG TGGGCGGCCA 
AAGCCCAGCA TCACCTGGAA GGGTCCGGAT CAGAACATCC TAGACACTGA CAACAGTTCA 
GCAACATACA CCATCTCCTC CTGTGATTCT GGGGAAAGCA CCCTCAAGAT CTGCAACCTG 
ATGCCCCAGG ATAGTGGGAT TTATACCTGC ATAGCAGCAA ACGACCACGG GACTGCATCC 
ACATCTGCTA CAGTTAAGGT GCAAGGTGTC CCCGCAGCCC CCAACCGCCC AATCGCACAG 
GAGAGGAGCT GCACCTCCGT CATCCTCCGC TGGCTGCCCC CAGCCAGCAC CGGAAACTGC 
ACTATTTCGG GGTACACAGT GGAGTATCGC GAGGAAGGTT CCCAGGTCTG GCAGCAATCC 
GTAGCTTCGA CCTTGGACAC TTACCTGGTC ATTGAAGACC TTAGTCCTGG GTGCCCTTAT 
CAGTTCAGGG TCAGTGCCAG CAACCCCTGG GGAATCAGCC TTCCCAGTGA GCCCTCGGAG 
TTTGTGCGCC TTCCAGAGTA CGATGCAGCC GCCGACGGTG CCACCATTTC TTGGAAGGAA 
AATTTTGATT CTGCTTACAC TGAACTGAAC GAGATTGGCA GAGGCCGTTT CTCTATTGTA 
AAGAAATGCA TTCACAAAGC TACCCGCAAA GATGTTGCTG TGAAATTTGT GAGCAAAAAA 
ATGAAGAAGA AGGAGCAGGC TGCTCACGAG GCCGCCCTGC TCCAGCACCT GCAGCACCCT 
CAGTATGTCA CGCTCCATGA CACCTACGAG TCCCCCACAT CCTACATCCT GATTTTGGAA 
CTGATGGATG ACGGCCGACT CTTGGACTAC CTTATGAATC ATGATGAGCT GATGGAAGAA 
AAGGTAGCTT TCTACATTCG AGACATCATG GAAGCTCTGC AGTACCTGCA CAACTGTAGG 
GTCGCACATT TGGACATAAA GCCCGAAAAC CTGCTCATTG ACCTCCGGAT CCCAGTGCCT 
CGAGTGAAGC TCATCGACTT GGAAGACGCT GTCCAGATCT CAGGCCACTT CCATATCCAC 
CATCTGCTGG GGAACCCTGA GTTCGCTGCC CCCGAAGTGA TCCAAGGCAT CCCTGTCTCC 
CTGGGCACAG ACATCTGGAG CATCGGGGTT CTGACATATG TCATGCTTAG TGGGGTCTCC 
CCCTTCTTAG ATGAGAGCAA AGAGGAAACC TGTATCAACG TGTGCAGGGT GGACTTTAGC 
TTCCCCCACG AGTACTTCTG CGGTGTGAGC AATGCTGCCA GGGATTTCAT CAACGTAATC 
TTACAGGAAG ACTTCCGGAG GCGGCCCACA GCAGCCACGT GCCTGCAGCA TCCCTGGCTA 
CAGCCCCACA ACGGCAGCTA CTCCAAGATC CCCTTGGACA CCTCCCGTCT GGCCTGCTTC 
ATAGAACGCC GCAAGCACCA GAATGATGTG CGGCCCATTC CCAATGTCAA GAGTTACATC 
GTCAATAGGG TGAACCAAGG GACCTCGCTA TCCCACAACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244256.1
CDS	3335..12319
Translation	MTDRFWDQWYLWYLRLLRLLDRGILRSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245954.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245954.2

ORF Insert Sequence:

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGTA TCCTGAGATC TTTTCGGAAT GATGGTTTGA AAGCTTCTGA TGTCCTTCCT 
ATCCTAAAGG AGAAAGTGGC CTTTGTGTCT GGGGGGCGTG ACAAGCGAGG TGGACCCATC 
CTGACCTTCC CTGCCCGAAG CAATCATGAC AGAATAAGAC AGGAAGACCT ACGGAAACTT 
GTGACGTATT TGGCCAGCGT GCCAAGTGAG GACGTTTGCA AACGTGGCTT CACCGTCATC 
ATCGACATGC GGGGCTCCAA GTGGGATCTC ATCAAGCCCC TCCTCAAGAC GCTGCAGGAA 
GCCTTCCCCG CCGAGATCCA TGTGGCCCTC ATCATCAAGC CTGACAACTT CTGGCAGAAG 
CAGAAGACCA ACTTTGGCAG CTCCAAATTC ATCTTTGAGA CAAGCATGGT GTCTGTGGAG 
GGCCTCACGA AGCTGGTGGA CCCCTCCCAG CTGACAGAGG AGTTTGACGG CTCCCTGGAT 
TACAACCATG AGGAGTGGAT TGAGTTGCGC CTCTCCCTGG AGGAGTTCTT CAACAGTGCG 
GTCCACCTGC TCTCACGCCT TGAGGACCTG CAGGAGATGC TGGCCCGGAA GGAGTTCCCT 
GTGGATGTGG AGGGCTCTCG GAGGCTTATC GACGAGCACA CGCAACTTAA GAAAAAGGTG 
CTGAAGGCCC CCGTGGAAGA GCTGGACCGG GAGGGGCAGC GTCTACTGCA GTGCATCCGC 
TGCAGCGATG GCTTCTCAGG GCGCAACTGC ATCCCAGGAA GTGCCGACTT CCAGAGCCTT 
GTACCCAAGA TCACCAGCCT CCTGGACAAG CTGCATTCCA CCCGACAACA CCTGCACCAA 
ATGTGGCACG TGCGAAAGCT CAAGCTGGAT CAGTGCTTCC AGCTGCGGCT CTTCGAGCAG 
GATGCTGAGA AGATGTTCGA CTGGATAAGC CACAACAAGG AGTTATTTCT TCAGAGCCAC 
ACAGAGATCG GAGTCAGCTA CCAGCATGCA CTAGACCTCC AGACACAGCA CAATCACTTT 
GCCATGAACT CCATGAATGC CTATGTCAAC ATCAACCGCA TCATGTCTGT CGCTTCCCGC 
CTCTCAGAGG CCGGCCATTA TGCCTCCCAG CAAATCAAGC AGATCTCCAC GCAGCTAGAC 
CAGGAGTGGA AGAGCTTTGC CGCTGCCCTG GATGAACGCA GTACAATCCT GGCCATGTCT 
GCTGTGTTCC ACCAGAAGGC AGAGCAGTTC CTGTCAGGCG TGGATGCCTG GTGTAAGATG 
TGCAGCGAAG GCGGCCTTCC TTCTGAGATG CAGGACCTGG AGCTGGCAAT CCACCATCAC 
CAGTCCTTAT ATGAGCAGGT GACCCAGGCC TACACAGAGG TCAGCCAGGA TGGCAAAGCA 
CTACTGGATG TCCTGCAGCG TCCCCTGAGT CCTGGGAACT CTGAGTCCCT CACAGCCACA 
GCCAACTACT CCAAGGCGGT CCACCAGGTG CTGGATGTGG TCCACGAGGT GCTGCACCAT 
CAGCGACGGC TCGAGAGCAT CTGGCAGCAC CGCAAGGTGC GGCTTCACCA GCGGCTGCAG 
CTGTGTGTCT TCCAGCAGGA TGTGCAGCAG GTGCTGGACT GGATTGAAAA CCATGGCGAG 
GCCTTTCTCA GCAAACACAC TGGGGTTGGC AAGTCCTTGC ATCGAGCACG TGCACTACAG 
AAGAGGCATG ATGACTTTGA AGAAGTGGCA CAGAATACAT ACACCAATGC GGACAAGCTC 
CTAGAAGCAG CGGAGCAGCT GGCTCAGACT GGGGAATGTG ACCCGGAGGA GATCTACAAG 
GCAGCCCGGC ACCTGGAGGT GCGCATCCAA GACTTTGTGC GCAGAGTGGA GCAGCGGAAG 
CTGCTTCTGG ACATGTCGGT CTCTTTCCAC ACGCACACCA AAGAGCTGTG GACATGGATG 
GAGGACCTTC AGAAGGAGGT GCTGGAGGAT GTCTGTGCAG ACTCTGTGGA TGCGGTCCAG 
GAGCTGATCA AGCAGTTCCA GCAGCAGCAG ACCGCCACCC TGGATGCCAC TCTCAATGTC 
ATCAAGGAAG GCGAAGACCT TATCCAGCAG CTCAGGTCAG CGCCTCCCTC CCTGGGGGAG 
CCCACCGAGG CCAGGGACTC AGCGATGTCC AACAACAAGA CACCCCACAG TAGCTCTATC 
AGCCATATTG AGTCTGTCCT CCAGCAGCTG GACGATGCTC AGGTACAGAT GGAAGAGCTG 
TTTCACGAGC GGAAGATCAA GCTGGACATC TTCCTACAGC TGCGCATCTT TGAACAGTAC 
ACCATTGAGG TCACTGCAGA GCTGGATGCC TGGAACGAGG ACTTGCTCCG GCAGATGAAT 
GATTTCAACA CAGAGGATCT GACCCTGGCT GAGCAGCGGC TACAGCGCCA CACAGAACGG 
AAGCTTGCCA TGAACAACAT GACGTTCGAG GTCATCCAGC AGGGCCAGGA CCTGCACCAG 
TACATCATGG AAGTGCAAGC GTCAGGAATC GAGTTAATCT GTGAGAAGGA CCTTGACCTG 
GCAGCTCAGG TGCAGGAGCT GCTGGAGTTT CTCCATGAGA AGCAGCACGA GCTGGAGCTC 
AATGCGGAGC AGACGCACAA GCGGCTAGAG CAGTGTCTCC AGCTGAGGCA CCTCCAGGCT 
GAGGTCAAAC AGGTTCTGGG ATGGATCCGC AATGGAGAGT CGATGCTCAA CGCCAGCCTG 
GTCAACGCCA GCTCTTTGTC GGAGGCAGAG CAGCTGCAGC GGGAGCATGA ACAGTTCCAG 
CTAGCCATCG AGTCCCTCTT TCATGCCACT TCCTTGCAGA AGACGCACCA GAGCGCCCTG 
CAGGTACAGC AGAAGGCAGA GGCACTGCTC CAGGCCGGCC ACTACGACGC AGACGCCATC 
CGGGAATGTG CTGAGAAGGT GGCCCTCCAC TGGCAACAGC TCATGCTAAA GATGGAGGAC 
CGGCTGAAGC TTGTCAACGC CTCTGTGGCC TTTTACAAGA CCTCTGAGCA GGTGTGCAGT 
GTTCTGGAGA GCTTGGAGCA GGAGTACCGG AGAGACGAGG ACTGGTGTGG TGGGAGAGAC 
AAGCTGGGGC CGGCGGCAGA GATGGACCAC GTTATTCCAC TCCTCAGCAA GCACCTGGAG 
CAGAAGGAGG CCTTCCTGAA GGCCTGCACG CTGGCAAGGC GGAACGCCGA GGTGTTCCTC 
AAATACATCC ACAGGAACAA TGTGAGCATG CCTAGTGTTG CCAGCCACAC CCGGGGGCCT 
GAACAGCAAG TAAAAGCCAT CTTGAGCGAG CTCTTGCAGA GGGAGAATCG AGTCCTGCAC 
TTCTGGACCT TGAAGAAGCG GCGGTTAGAC CAGTGTCAGC AATATGTGGT GTTTGAGCGC 
AGTGCTAAGC AGGCACTGGA CTGGATCCAA GAAACAGGAG AATATTACCT CTCAACACAT 
ACCTCCACCG GAGAGACCAC GGAGGAGACT CAGGAGCTGC TGAAAGAGTA TGGAGAATTC 
AGGGTGCCTG CCAAGCAAAC GAAGGAGAAG GTGAAACTTC TGATCCAGCT AGCAGACAGC 
TTTGTGGAAA AGGGCCACAT CCACGCCACA GAGATACGGA AATGGGTGAC CACGGTGGAC 
AAGCACTACC GCGATTTCTC CCTGAGGATG GGGAAGTACC GGTACTCGCT GGAGAAGGCG 
CTGGGGGTCA ACACGGAGGA TAACAAGGAC CTAGAGCTGG ACATCATCCC AGCAAGCCTT 
TCGGATCGGG AGGTCAAGCT CCGGGACGCT AACCATGAGA TCAATGAAGA GAAACGGAAG 
TCAGCTCGAA AGAAAGAGTT CATTATGGCT GAACTGCTCC AAACTGAGAA GGCTTATGTA 
AGGGACTTGC ACGAGTGCTT GGAGACCTAC CTGTGGGAGA TGACTAGTGG TGTGGAAGAG 
ATACCCCCCG GCATCCTCAA TAAGGAACAT ATCATCTTCG GCAACATCCA GGAGATCTAT 
GACTTCCATA ACAACATCTT CCTCAAAGAG CTGGAGAAGT ACGAGCAGCT GCCTGAGGAT 
GTGGGACACT GCTTTGTTAC CTGGGCAGAC AAATTTCAGA TGTATGTCAC CTACTGTAAA 
AACAAGCCTG ATTCCAACCA GCTGATCCTG GAGCATGCGG GCACCTTCTT TGATGAGATC 
CAGCAGCGGC ATGGCCTGGC CAACTCCATC TCCTCCTACC TAATTAAGCC TGTCCAGAGG 
GTTACCAAGT ACCAGCTGCT CCTGAAGGAA CTTCTAACTT GCTGTGAAGA AGGGAAAGGA 
GAGCTGAAGG ATGGCCTGGA GGTGATGCTC AGTGTCCCCA AGAAAGCCAA CGATGCCATG 
CATGTCAGCA TGCTGGAAGG GTTCGATGAA AACTTGGATG TACAGGGGGA GTTGATTCTC 
CAGGATGCCT TTCAAGTGTG GGACCCAAAG TCGCTGATCC GGAAGGGGCG GGAGCGCCAT 
CTGTTCCTCT TTGAGATCTC CTTGGTGTTT AGCAAAGAGA TCAAAGACTC TTCAGGACAC 
ACGAAATATG TTTACAAGAA CAAGCTACTG ACCTCAGAGC TGGGTGTGAC CGAGCATGTA 
GAAGGTGACC CCTGCAAATT CGCCTTGTGG TCTGGGCGCA CCCCATCCTC AGACAATAAA 
ACAGTGCTCA AAGCTTCCAA CATCGAAACC AAGCAGGAAT GGATCAAGAA CATTCGAGAA 
GTGATTCAGG AGAGGATCAT TCATCTTAAA GGCGCTTTAA AGGAGCCAAT TCAGCTCCCC 
AAAACACCAG CCAAACTGAG GAACAATAGT AAGAGGGATG GGGTAGAGGA TGGTGACAGC 
CAAGGGGATG GGAGCAGCCA GCCAGATACC ATATCCATTG CCTCCAGGAC CTCTCAGAAC 
ACAGTGGAAA GTGACAAGCT CTCAGGTGGA TGTGAGCTGA CCGTGGTCCT CCAGGACTTC 
AGCGCAGGCC ACAGCAGCGA GCTGAGCATC CAGGTGGGCC AGACGGTGGA GCTGCTGGAA 
CGACCGAGTG AGAGGCCTGG CTGGTGTCTG GTCCGCACCA CAGAGCGGAG CCCCCCACAG 
GAAGGCCTGG TACCCAGCAG CGCCCTGTGC ATCTCACACT CTCGCAGCAG CGTGGAAATG 
GACTGCTTCT TCCCCTTGAA AGATTCATAC TCTCATTCCT CCAGTGAGAA TGGAGGCAAA 
TCCGAGTCCG TGGCCCACCT GCAGTCTCAG CCCTCCCTGA ACTCCATCCA CAGTTCCCCT 
GGTCCCAAGC GCTCCACCAA CACCCTCAAA AAGTGGCTGA CCAGCCCCGT GCGCCGCCTC 
AACAGCGGAA AAGCCGACGG GAACATCAAA AAGCAGAAGA AAGTCCGGGA TGGGCGGAAG 
AGCTTCGATC TGGGATCGCC CAAGCCTGGG GATGAGACGA CACCTCAGGG AGACAGCGCT 
GACGAGAAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GTCGTCTTTG 
CTAGCAGCCC GGCAGGCTCC CCCTGACGTG CCTACTGCTG CAGACCTTGT CAGTGCAATA 
GAAAAGTTGG TCAAAAATAA GCTGACTCTG GAAGGAGGCT CTTACCGGGG GAGCTTGAAG 
GACCCTACAG GCTGCCTGAA TGAGGGGATG ACCCCTCCCA CACCTCCTAG AAACCTGGAA 
GAAGAACAGA AAGCCAAGGC GCTGAGAGGC AGGATGTTTG TCCTGAATGA GCTGGTACAG 
ACAGAGAAGG ACTACGTCAA GGACCTGGGG ATTGTGGTGG AGGGCTTCAT GAAGAGAATA 
GAAGAGAAGG GGGTCCCTGA GGACATGCGA GGGAAGGAAA AGATCGTGTT TGGAAATATC 
CACCAGATTT ACGACTGGCA TAAAGATTTT TTCCTGGCTG AACTGGAAAA ATGTATCCAA 
GAGCAAGACA GATTGGCCCA GCTCTTCATT AAACACGAGC GGAAGCTGCA CATCTACGTG 
TGGTACTGCC AGAACAAGCC GCGTTCCGAG TACATCGTGG CTGAGTACGA TGCCTATTTT 
GAGGAGGTTA AACAAGAGAT CAATCAAAGG CTGACCCTTA GTGACTTCCT GATCAAGCCC 
ATTCAGAGAA TAACAAAGTA CCAGCTGCTA CTCAAGGACT TCCTGCGATA CAGCGAGAAG 
GCTGGTTTGG AGTGTTCCGA TATTGAGAAA GCAGTGGAGT TAATGTGCCT TGTCCCCAAG 
CGCTGTAATG ACATGATGAA TCTGGGACGC CTGCAGGGCT TCGAGGGCAC CCTGACCGCC 
CAGGGCAAGC TACTGCAGCA GGACACATTC TATGTGATCG AGCTGGATGC CGGCATGCAG 
TCCCGGACCA AGGAGAGGCG TGTGTTTCTC TTTGAGCAGA TTGTCATCTT CAGTGAACTG 
CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC ATGTTCAAAA GAAGCATCAA GATGAATTAC 
TTGGTCCTGG AAGACAATGT GGATGGTGAC CCCTGCAAAT TTGCACTCAT GAACAGGGAG 
ACTTCAGAAA GGGTCATTCT ACAGGCTGCC AACTCAGACA TCCAGCAGGC CTGGGTGCAG 
GACATCAACC AAGTCCTAGA AACACAGAGG GACTTCTTAA ATGCACTACA GTCACCCATT 
GAGTATCAGC GGAAGGAGAG AAGCACAGCT GTGATCCGGT CCCAGCCCCC GAGGGTTCCA 
CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC 
TCCAGCTATA ACCCGCCTCT GCCACCCCTG AAGATATCTA CCTCTAATGG CAGTCCAGGG 
TTCGACTACC ATCAGCCTGG GGACAAGTTT GACGCTAGCA AGCAGAACGA CCTGGGAGGC 
TGCAATGGGA CATCAACCAT GACTGTAATC AAAGATTACT ATGCACTGAA GGAGAATGAA 
ATCTGCGTGA GCCAAGGTGA GGTGGTACAG GTCCTCGCCG TCAACCAGCA GAACATGTGT 
CTGGTGTACC AGCCTGCCAG CGACCATTCC CCGGCAGCAG AGGGCTGGGT CCCAGGCAGC 
ATCCTGGCGC CCCTCGCCAA AGCCACAGCA GCGGCCGAAA GTAGTGACGG GAGCATCAAG 
AAGTCATGCT CTTGGCACAC TCTACGCATG AGAAAGCGGG CAGACGTGGA GAACTCGGGT 
AAAAATGAAG CCACAGGGCC TCGTAAACCC AAGGATATTC TGGGCAACAA AGTCTCTGTT 
AAAGAGACGA ACAGTTCCGA GGAGTCAGAG TGTGATGATC TTGACCCTAA TACTAGTATG 
GAGATCTTGA ATCCAAATTT CATCCAGGAA GTGGCTCCAG AATTCCTCGT GCCCTTAGTA 
GATGTGACCT GTTTGCTTGG AGACACAGTG CTACTGCAGT GTAAAGCCTG TGGGCGGCCA 
AAGCCCAGCA TCACCTGGAA GGGTCCGGAT CAGAACATCC TAGACACTGA CAACAGTTCA 
GCAACATACA CCATCTCCTC CTGTGATTCT GGGGAAAGCA CCCTCAAGAT CTGCAACCTG 
ATGCCCCAGG ATAGTGGGAT TTATACCTGC ATAGCAGCAA ACGACCACGG GACTGCATCC 
ACATCTGCTA CAGTTAAGGT GCAAGGTGTC CCCGCAGCCC CCAACCGCCC AATCGCACAG 
GAGAGGAGCT GCACCTCCGT CATCCTCCGC TGGCTGCCCC CAGCCAGCAC CGGAAACTGC 
ACTATTTCGG GGTACACAGT GGAGTATCGC GAGGAAGGTT CCCAGGTCTG GCAGCAATCC 
GTAGCTTCGA CCTTGGACAC TTACCTGGTC ATTGAAGACC TTAGTCCTGG GTGCCCTTAT 
CAGTTCAGGG TCAGTGCCAG CAACCCCTGG GGAATCAGCC TTCCCAGTGA GCCCTCGGAG 
TTTGTGCGCC TTCCAGAGTA CGATGCAGCC GCCGACGGTG CCACCATTTC TTGGAAGGAA 
AATTTTGATT CTGCTTACAC TGAACTGAAC GAGATTGGCA GAGGCCGTTT CTCTATTGTA 
AAGAAATGCA TTCACAAAGC TACCCGCAAA GATGTTGCTG TGAAATTTGT GAGCAAAAAA 
ATGAAGAAGA AGGAGCAGGC TGCTCACGAG GCCGCCCTGC TCCAGCACCT GCAGCACCCT 
CAGTATGTCA CGCTCCATGA CACCTACGAG TCCCCCACAT CCTACATCCT GATTTTGGAA 
CTGATGGATG ACGGCCGACT CTTGGACTAC CTTATGAATC ATGATGAGCT GATGGAAGAA 
AAGGTAGCTT TCTACATTCG AGACATCATG GAAGCTCTGC AGTACCTGCA CAACTGTAGG 
GTCGCACATT TGGACATAAA GCCCGAAAAC CTGCTCATTG ACCTCCGGAT CCCAGTGCCT 
CGAGTGAAGC TCATCGACTT GGAAGACGCT GTCCAGATCT CAGGCCACTT CCATATCCAC 
CATCTGCTGG GGAACCCTGA GTTCGCTGCC CCCGAAGTGA TCCAAGGCAT CCCTGTCTCC 
CTGGGCACAG ACATCTGGAG CATCGGGGTT CTGACATATG TCATGCTTAG TGGGGTCTCC 
CCCTTCTTAG ATGAGAGCAA AGAGGAAACC TGTATCAACG TGTGCAGGGT GGACTTTAGC 
TTCCCCCACG AGTACTTCTG CGGTGTGAGC AATGCTGCCA GGGATTTCAT CAACGTAATC 
TTACAGGAAG ACTTCCGGAG GCGGCCCACA GCAGCCACGT GCCTGCAGCA TCCCTGGCTA 
CAGCCCCACA ACGGCAGCTA CTCCAAGATC CCCTTGGACA CCTCCCGTCT GGCCTGCTTC 
ATAGAACGCC GCAAGCACCA GAATGATGTG CGGCCCATTC CCAATGTCAA GAGTTACATC 
GTCAATAGGG TGAACCAAGG GACCTCGCTA TCCCACAACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245954.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244256.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X4, mRNA.

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CloneID	OMu75310
Clone ID Related Accession (Same CDS sequence)	XM_011245954.2 , XM_011245954.3
Accession Version	XM_011245954.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245954.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGTA TCCTGAGATC TTTTCGGAAT GATGGTTTGA AAGCTTCTGA TGTCCTTCCT 
ATCCTAAAGG AGAAAGTGGC CTTTGTGTCT GGGGGGCGTG ACAAGCGAGG TGGACCCATC 
CTGACCTTCC CTGCCCGAAG CAATCATGAC AGAATAAGAC AGGAAGACCT ACGGAAACTT 
GTGACGTATT TGGCCAGCGT GCCAAGTGAG GACGTTTGCA AACGTGGCTT CACCGTCATC 
ATCGACATGC GGGGCTCCAA GTGGGATCTC ATCAAGCCCC TCCTCAAGAC GCTGCAGGAA 
GCCTTCCCCG CCGAGATCCA TGTGGCCCTC ATCATCAAGC CTGACAACTT CTGGCAGAAG 
CAGAAGACCA ACTTTGGCAG CTCCAAATTC ATCTTTGAGA CAAGCATGGT GTCTGTGGAG 
GGCCTCACGA AGCTGGTGGA CCCCTCCCAG CTGACAGAGG AGTTTGACGG CTCCCTGGAT 
TACAACCATG AGGAGTGGAT TGAGTTGCGC CTCTCCCTGG AGGAGTTCTT CAACAGTGCG 
GTCCACCTGC TCTCACGCCT TGAGGACCTG CAGGAGATGC TGGCCCGGAA GGAGTTCCCT 
GTGGATGTGG AGGGCTCTCG GAGGCTTATC GACGAGCACA CGCAACTTAA GAAAAAGGTG 
CTGAAGGCCC CCGTGGAAGA GCTGGACCGG GAGGGGCAGC GTCTACTGCA GTGCATCCGC 
TGCAGCGATG GCTTCTCAGG GCGCAACTGC ATCCCAGGAA GTGCCGACTT CCAGAGCCTT 
GTACCCAAGA TCACCAGCCT CCTGGACAAG CTGCATTCCA CCCGACAACA CCTGCACCAA 
ATGTGGCACG TGCGAAAGCT CAAGCTGGAT CAGTGCTTCC AGCTGCGGCT CTTCGAGCAG 
GATGCTGAGA AGATGTTCGA CTGGATAAGC CACAACAAGG AGTTATTTCT TCAGAGCCAC 
ACAGAGATCG GAGTCAGCTA CCAGCATGCA CTAGACCTCC AGACACAGCA CAATCACTTT 
GCCATGAACT CCATGAATGC CTATGTCAAC ATCAACCGCA TCATGTCTGT CGCTTCCCGC 
CTCTCAGAGG CCGGCCATTA TGCCTCCCAG CAAATCAAGC AGATCTCCAC GCAGCTAGAC 
CAGGAGTGGA AGAGCTTTGC CGCTGCCCTG GATGAACGCA GTACAATCCT GGCCATGTCT 
GCTGTGTTCC ACCAGAAGGC AGAGCAGTTC CTGTCAGGCG TGGATGCCTG GTGTAAGATG 
TGCAGCGAAG GCGGCCTTCC TTCTGAGATG CAGGACCTGG AGCTGGCAAT CCACCATCAC 
CAGTCCTTAT ATGAGCAGGT GACCCAGGCC TACACAGAGG TCAGCCAGGA TGGCAAAGCA 
CTACTGGATG TCCTGCAGCG TCCCCTGAGT CCTGGGAACT CTGAGTCCCT CACAGCCACA 
GCCAACTACT CCAAGGCGGT CCACCAGGTG CTGGATGTGG TCCACGAGGT GCTGCACCAT 
CAGCGACGGC TCGAGAGCAT CTGGCAGCAC CGCAAGGTGC GGCTTCACCA GCGGCTGCAG 
CTGTGTGTCT TCCAGCAGGA TGTGCAGCAG GTGCTGGACT GGATTGAAAA CCATGGCGAG 
GCCTTTCTCA GCAAACACAC TGGGGTTGGC AAGTCCTTGC ATCGAGCACG TGCACTACAG 
AAGAGGCATG ATGACTTTGA AGAAGTGGCA CAGAATACAT ACACCAATGC GGACAAGCTC 
CTAGAAGCAG CGGAGCAGCT GGCTCAGACT GGGGAATGTG ACCCGGAGGA GATCTACAAG 
GCAGCCCGGC ACCTGGAGGT GCGCATCCAA GACTTTGTGC GCAGAGTGGA GCAGCGGAAG 
CTGCTTCTGG ACATGTCGGT CTCTTTCCAC ACGCACACCA AAGAGCTGTG GACATGGATG 
GAGGACCTTC AGAAGGAGGT GCTGGAGGAT GTCTGTGCAG ACTCTGTGGA TGCGGTCCAG 
GAGCTGATCA AGCAGTTCCA GCAGCAGCAG ACCGCCACCC TGGATGCCAC TCTCAATGTC 
ATCAAGGAAG GCGAAGACCT TATCCAGCAG CTCAGGTCAG CGCCTCCCTC CCTGGGGGAG 
CCCACCGAGG CCAGGGACTC AGCGATGTCC AACAACAAGA CACCCCACAG TAGCTCTATC 
AGCCATATTG AGTCTGTCCT CCAGCAGCTG GACGATGCTC AGGTACAGAT GGAAGAGCTG 
TTTCACGAGC GGAAGATCAA GCTGGACATC TTCCTACAGC TGCGCATCTT TGAACAGTAC 
ACCATTGAGG TCACTGCAGA GCTGGATGCC TGGAACGAGG ACTTGCTCCG GCAGATGAAT 
GATTTCAACA CAGAGGATCT GACCCTGGCT GAGCAGCGGC TACAGCGCCA CACAGAACGG 
AAGCTTGCCA TGAACAACAT GACGTTCGAG GTCATCCAGC AGGGCCAGGA CCTGCACCAG 
TACATCATGG AAGTGCAAGC GTCAGGAATC GAGTTAATCT GTGAGAAGGA CCTTGACCTG 
GCAGCTCAGG TGCAGGAGCT GCTGGAGTTT CTCCATGAGA AGCAGCACGA GCTGGAGCTC 
AATGCGGAGC AGACGCACAA GCGGCTAGAG CAGTGTCTCC AGCTGAGGCA CCTCCAGGCT 
GAGGTCAAAC AGGTTCTGGG ATGGATCCGC AATGGAGAGT CGATGCTCAA CGCCAGCCTG 
GTCAACGCCA GCTCTTTGTC GGAGGCAGAG CAGCTGCAGC GGGAGCATGA ACAGTTCCAG 
CTAGCCATCG AGTCCCTCTT TCATGCCACT TCCTTGCAGA AGACGCACCA GAGCGCCCTG 
CAGGTACAGC AGAAGGCAGA GGCACTGCTC CAGGCCGGCC ACTACGACGC AGACGCCATC 
CGGGAATGTG CTGAGAAGGT GGCCCTCCAC TGGCAACAGC TCATGCTAAA GATGGAGGAC 
CGGCTGAAGC TTGTCAACGC CTCTGTGGCC TTTTACAAGA CCTCTGAGCA GGTGTGCAGT 
GTTCTGGAGA GCTTGGAGCA GGAGTACCGG AGAGACGAGG ACTGGTGTGG TGGGAGAGAC 
AAGCTGGGGC CGGCGGCAGA GATGGACCAC GTTATTCCAC TCCTCAGCAA GCACCTGGAG 
CAGAAGGAGG CCTTCCTGAA GGCCTGCACG CTGGCAAGGC GGAACGCCGA GGTGTTCCTC 
AAATACATCC ACAGGAACAA TGTGAGCATG CCTAGTGTTG CCAGCCACAC CCGGGGGCCT 
GAACAGCAAG TAAAAGCCAT CTTGAGCGAG CTCTTGCAGA GGGAGAATCG AGTCCTGCAC 
TTCTGGACCT TGAAGAAGCG GCGGTTAGAC CAGTGTCAGC AATATGTGGT GTTTGAGCGC 
AGTGCTAAGC AGGCACTGGA CTGGATCCAA GAAACAGGAG AATATTACCT CTCAACACAT 
ACCTCCACCG GAGAGACCAC GGAGGAGACT CAGGAGCTGC TGAAAGAGTA TGGAGAATTC 
AGGGTGCCTG CCAAGCAAAC GAAGGAGAAG GTGAAACTTC TGATCCAGCT AGCAGACAGC 
TTTGTGGAAA AGGGCCACAT CCACGCCACA GAGATACGGA AATGGGTGAC CACGGTGGAC 
AAGCACTACC GCGATTTCTC CCTGAGGATG GGGAAGTACC GGTACTCGCT GGAGAAGGCG 
CTGGGGGTCA ACACGGAGGA TAACAAGGAC CTAGAGCTGG ACATCATCCC AGCAAGCCTT 
TCGGATCGGG AGGTCAAGCT CCGGGACGCT AACCATGAGA TCAATGAAGA GAAACGGAAG 
TCAGCTCGAA AGAAAGAGTT CATTATGGCT GAACTGCTCC AAACTGAGAA GGCTTATGTA 
AGGGACTTGC ACGAGTGCTT GGAGACCTAC CTGTGGGAGA TGACTAGTGG TGTGGAAGAG 
ATACCCCCCG GCATCCTCAA TAAGGAACAT ATCATCTTCG GCAACATCCA GGAGATCTAT 
GACTTCCATA ACAACATCTT CCTCAAAGAG CTGGAGAAGT ACGAGCAGCT GCCTGAGGAT 
GTGGGACACT GCTTTGTTAC CTGGGCAGAC AAATTTCAGA TGTATGTCAC CTACTGTAAA 
AACAAGCCTG ATTCCAACCA GCTGATCCTG GAGCATGCGG GCACCTTCTT TGATGAGATC 
CAGCAGCGGC ATGGCCTGGC CAACTCCATC TCCTCCTACC TAATTAAGCC TGTCCAGAGG 
GTTACCAAGT ACCAGCTGCT CCTGAAGGAA CTTCTAACTT GCTGTGAAGA AGGGAAAGGA 
GAGCTGAAGG ATGGCCTGGA GGTGATGCTC AGTGTCCCCA AGAAAGCCAA CGATGCCATG 
CATGTCAGCA TGCTGGAAGG GTTCGATGAA AACTTGGATG TACAGGGGGA GTTGATTCTC 
CAGGATGCCT TTCAAGTGTG GGACCCAAAG TCGCTGATCC GGAAGGGGCG GGAGCGCCAT 
CTGTTCCTCT TTGAGATCTC CTTGGTGTTT AGCAAAGAGA TCAAAGACTC TTCAGGACAC 
ACGAAATATG TTTACAAGAA CAAGCTACTG ACCTCAGAGC TGGGTGTGAC CGAGCATGTA 
GAAGGTGACC CCTGCAAATT CGCCTTGTGG TCTGGGCGCA CCCCATCCTC AGACAATAAA 
ACAGTGCTCA AAGCTTCCAA CATCGAAACC AAGCAGGAAT GGATCAAGAA CATTCGAGAA 
GTGATTCAGG AGAGGATCAT TCATCTTAAA GGCGCTTTAA AGGAGCCAAT TCAGCTCCCC 
AAAACACCAG CCAAACTGAG GAACAATAGT AAGAGGGATG GGGTAGAGGA TGGTGACAGC 
CAAGGGGATG GGAGCAGCCA GCCAGATACC ATATCCATTG CCTCCAGGAC CTCTCAGAAC 
ACAGTGGAAA GTGACAAGCT CTCAGGTGGA TGTGAGCTGA CCGTGGTCCT CCAGGACTTC 
AGCGCAGGCC ACAGCAGCGA GCTGAGCATC CAGGTGGGCC AGACGGTGGA GCTGCTGGAA 
CGACCGAGTG AGAGGCCTGG CTGGTGTCTG GTCCGCACCA CAGAGCGGAG CCCCCCACAG 
GAAGGCCTGG TACCCAGCAG CGCCCTGTGC ATCTCACACT CTCGCAGCAG CGTGGAAATG 
GACTGCTTCT TCCCCTTGAA AGATTCATAC TCTCATTCCT CCAGTGAGAA TGGAGGCAAA 
TCCGAGTCCG TGGCCCACCT GCAGTCTCAG CCCTCCCTGA ACTCCATCCA CAGTTCCCCT 
GGTCCCAAGC GCTCCACCAA CACCCTCAAA AAGTGGCTGA CCAGCCCCGT GCGCCGCCTC 
AACAGCGGAA AAGCCGACGG GAACATCAAA AAGCAGAAGA AAGTCCGGGA TGGGCGGAAG 
AGCTTCGATC TGGGATCGCC CAAGCCTGGG GATGAGACGA CACCTCAGGG AGACAGCGCT 
GACGAGAAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GTCGTCTTTG 
CTAGCAGCCC GGCAGGCTCC CCCTGACGTG CCTACTGCTG CAGACCTTGT CAGTGCAATA 
GAAAAGTTGG TCAAAAATAA GCTGACTCTG GAAGGAGGCT CTTACCGGGG GAGCTTGAAG 
GACCCTACAG GCTGCCTGAA TGAGGGGATG ACCCCTCCCA CACCTCCTAG AAACCTGGAA 
GAAGAACAGA AAGCCAAGGC GCTGAGAGGC AGGATGTTTG TCCTGAATGA GCTGGTACAG 
ACAGAGAAGG ACTACGTCAA GGACCTGGGG ATTGTGGTGG AGGGCTTCAT GAAGAGAATA 
GAAGAGAAGG GGGTCCCTGA GGACATGCGA GGGAAGGAAA AGATCGTGTT TGGAAATATC 
CACCAGATTT ACGACTGGCA TAAAGATTTT TTCCTGGCTG AACTGGAAAA ATGTATCCAA 
GAGCAAGACA GATTGGCCCA GCTCTTCATT AAACACGAGC GGAAGCTGCA CATCTACGTG 
TGGTACTGCC AGAACAAGCC GCGTTCCGAG TACATCGTGG CTGAGTACGA TGCCTATTTT 
GAGGAGGTTA AACAAGAGAT CAATCAAAGG CTGACCCTTA GTGACTTCCT GATCAAGCCC 
ATTCAGAGAA TAACAAAGTA CCAGCTGCTA CTCAAGGACT TCCTGCGATA CAGCGAGAAG 
GCTGGTTTGG AGTGTTCCGA TATTGAGAAA GCAGTGGAGT TAATGTGCCT TGTCCCCAAG 
CGCTGTAATG ACATGATGAA TCTGGGACGC CTGCAGGGCT TCGAGGGCAC CCTGACCGCC 
CAGGGCAAGC TACTGCAGCA GGACACATTC TATGTGATCG AGCTGGATGC CGGCATGCAG 
TCCCGGACCA AGGAGAGGCG TGTGTTTCTC TTTGAGCAGA TTGTCATCTT CAGTGAACTG 
CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC ATGTTCAAAA GAAGCATCAA GATGAATTAC 
TTGGTCCTGG AAGACAATGT GGATGGTGAC CCCTGCAAAT TTGCACTCAT GAACAGGGAG 
ACTTCAGAAA GGGTCATTCT ACAGGCTGCC AACTCAGACA TCCAGCAGGC CTGGGTGCAG 
GACATCAACC AAGTCCTAGA AACACAGAGG GACTTCTTAA ATGCACTACA GTCACCCATT 
GAGTATCAGC GGAAGGAGAG AAGCACAGCT GTGATCCGGT CCCAGCCCCC GAGGGTTCCA 
CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC 
TCCAGCTATA ACCCGCCTCT GCCACCCCTG AAGATATCTA CCTCTAATGG CAGTCCAGGG 
TTCGACTACC ATCAGCCTGG GGACAAGTTT GACGCTAGCA AGCAGAACGA CCTGGGAGGC 
TGCAATGGGA CATCAACCAT GACTGTAATC AAAGATTACT ATGCACTGAA GGAGAATGAA 
ATCTGCGTGA GCCAAGGTGA GGTGGTACAG GTCCTCGCCG TCAACCAGCA GAACATGTGT 
CTGGTGTACC AGCCTGCCAG CGACCATTCC CCGGCAGCAG AGGGCTGGGT CCCAGGCAGC 
ATCCTGGCGC CCCTCGCCAA AGCCACAGCA GCGGCCGAAA GTAGTGACGG GAGCATCAAG 
AAGTCATGCT CTTGGCACAC TCTACGCATG AGAAAGCGGG CAGACGTGGA GAACTCGGGT 
AAAAATGAAG CCACAGGGCC TCGTAAACCC AAGGATATTC TGGGCAACAA AGTCTCTGTT 
AAAGAGACGA ACAGTTCCGA GGAGTCAGAG TGTGATGATC TTGACCCTAA TACTAGTATG 
GAGATCTTGA ATCCAAATTT CATCCAGGAA GTGGCTCCAG AATTCCTCGT GCCCTTAGTA 
GATGTGACCT GTTTGCTTGG AGACACAGTG CTACTGCAGT GTAAAGCCTG TGGGCGGCCA 
AAGCCCAGCA TCACCTGGAA GGGTCCGGAT CAGAACATCC TAGACACTGA CAACAGTTCA 
GCAACATACA CCATCTCCTC CTGTGATTCT GGGGAAAGCA CCCTCAAGAT CTGCAACCTG 
ATGCCCCAGG ATAGTGGGAT TTATACCTGC ATAGCAGCAA ACGACCACGG GACTGCATCC 
ACATCTGCTA CAGTTAAGGT GCAAGGTGTC CCCGCAGCCC CCAACCGCCC AATCGCACAG 
GAGAGGAGCT GCACCTCCGT CATCCTCCGC TGGCTGCCCC CAGCCAGCAC CGGAAACTGC 
ACTATTTCGG GGTACACAGT GGAGTATCGC GAGGAAGGTT CCCAGGTCTG GCAGCAATCC 
GTAGCTTCGA CCTTGGACAC TTACCTGGTC ATTGAAGACC TTAGTCCTGG GTGCCCTTAT 
CAGTTCAGGG TCAGTGCCAG CAACCCCTGG GGAATCAGCC TTCCCAGTGA GCCCTCGGAG 
TTTGTGCGCC TTCCAGAGTA CGATGCAGCC GCCGACGGTG CCACCATTTC TTGGAAGGAA 
AATTTTGATT CTGCTTACAC TGAACTGAAC GAGATTGGCA GAGGCCGTTT CTCTATTGTA 
AAGAAATGCA TTCACAAAGC TACCCGCAAA GATGTTGCTG TGAAATTTGT GAGCAAAAAA 
ATGAAGAAGA AGGAGCAGGC TGCTCACGAG GCCGCCCTGC TCCAGCACCT GCAGCACCCT 
CAGTATGTCA CGCTCCATGA CACCTACGAG TCCCCCACAT CCTACATCCT GATTTTGGAA 
CTGATGGATG ACGGCCGACT CTTGGACTAC CTTATGAATC ATGATGAGCT GATGGAAGAA 
AAGGTAGCTT TCTACATTCG AGACATCATG GAAGCTCTGC AGTACCTGCA CAACTGTAGG 
GTCGCACATT TGGACATAAA GCCCGAAAAC CTGCTCATTG ACCTCCGGAT CCCAGTGCCT 
CGAGTGAAGC TCATCGACTT GGAAGACGCT GTCCAGATCT CAGGCCACTT CCATATCCAC 
CATCTGCTGG GGAACCCTGA GTTCGCTGCC CCCGAAGTGA TCCAAGGCAT CCCTGTCTCC 
CTGGGCACAG ACATCTGGAG CATCGGGGTT CTGACATATG TCATGCTTAG TGGGGTCTCC 
CCCTTCTTAG ATGAGAGCAA AGAGGAAACC TGTATCAACG TGTGCAGGGT GGACTTTAGC 
TTCCCCCACG AGTACTTCTG CGGTGTGAGC AATGCTGCCA GGGATTTCAT CAACGTAATC 
TTACAGGAAG ACTTCCGGAG GCGGCCCACA GCAGCCACGT GCCTGCAGCA TCCCTGGCTA 
CAGCCCCACA ACGGCAGCTA CTCCAAGATC CCCTTGGACA CCTCCCGTCT GGCCTGCTTC 
ATAGAACGCC GCAAGCACCA GAATGATGTG CGGCCCATTC CCAATGTCAA GAGTTACATC 
GTCAATAGGG TGAACCAAGG GACCTCGCTA TCCCACAACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244256.1
CDS	3337..12321
Translation	MTDRFWDQWYLWYLRLLRLLDRGILRSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245954.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245954.3

ORF Insert Sequence:

ATGACGGACC GCTTCTGGGA CCAGTGGTAT CTTTGGTATC TCCGCTTGCT TCGGCTTCTG 
GATCGAGGTA TCCTGAGATC TTTTCGGAAT GATGGTTTGA AAGCTTCTGA TGTCCTTCCT 
ATCCTAAAGG AGAAAGTGGC CTTTGTGTCT GGGGGGCGTG ACAAGCGAGG TGGACCCATC 
CTGACCTTCC CTGCCCGAAG CAATCATGAC AGAATAAGAC AGGAAGACCT ACGGAAACTT 
GTGACGTATT TGGCCAGCGT GCCAAGTGAG GACGTTTGCA AACGTGGCTT CACCGTCATC 
ATCGACATGC GGGGCTCCAA GTGGGATCTC ATCAAGCCCC TCCTCAAGAC GCTGCAGGAA 
GCCTTCCCCG CCGAGATCCA TGTGGCCCTC ATCATCAAGC CTGACAACTT CTGGCAGAAG 
CAGAAGACCA ACTTTGGCAG CTCCAAATTC ATCTTTGAGA CAAGCATGGT GTCTGTGGAG 
GGCCTCACGA AGCTGGTGGA CCCCTCCCAG CTGACAGAGG AGTTTGACGG CTCCCTGGAT 
TACAACCATG AGGAGTGGAT TGAGTTGCGC CTCTCCCTGG AGGAGTTCTT CAACAGTGCG 
GTCCACCTGC TCTCACGCCT TGAGGACCTG CAGGAGATGC TGGCCCGGAA GGAGTTCCCT 
GTGGATGTGG AGGGCTCTCG GAGGCTTATC GACGAGCACA CGCAACTTAA GAAAAAGGTG 
CTGAAGGCCC CCGTGGAAGA GCTGGACCGG GAGGGGCAGC GTCTACTGCA GTGCATCCGC 
TGCAGCGATG GCTTCTCAGG GCGCAACTGC ATCCCAGGAA GTGCCGACTT CCAGAGCCTT 
GTACCCAAGA TCACCAGCCT CCTGGACAAG CTGCATTCCA CCCGACAACA CCTGCACCAA 
ATGTGGCACG TGCGAAAGCT CAAGCTGGAT CAGTGCTTCC AGCTGCGGCT CTTCGAGCAG 
GATGCTGAGA AGATGTTCGA CTGGATAAGC CACAACAAGG AGTTATTTCT TCAGAGCCAC 
ACAGAGATCG GAGTCAGCTA CCAGCATGCA CTAGACCTCC AGACACAGCA CAATCACTTT 
GCCATGAACT CCATGAATGC CTATGTCAAC ATCAACCGCA TCATGTCTGT CGCTTCCCGC 
CTCTCAGAGG CCGGCCATTA TGCCTCCCAG CAAATCAAGC AGATCTCCAC GCAGCTAGAC 
CAGGAGTGGA AGAGCTTTGC CGCTGCCCTG GATGAACGCA GTACAATCCT GGCCATGTCT 
GCTGTGTTCC ACCAGAAGGC AGAGCAGTTC CTGTCAGGCG TGGATGCCTG GTGTAAGATG 
TGCAGCGAAG GCGGCCTTCC TTCTGAGATG CAGGACCTGG AGCTGGCAAT CCACCATCAC 
CAGTCCTTAT ATGAGCAGGT GACCCAGGCC TACACAGAGG TCAGCCAGGA TGGCAAAGCA 
CTACTGGATG TCCTGCAGCG TCCCCTGAGT CCTGGGAACT CTGAGTCCCT CACAGCCACA 
GCCAACTACT CCAAGGCGGT CCACCAGGTG CTGGATGTGG TCCACGAGGT GCTGCACCAT 
CAGCGACGGC TCGAGAGCAT CTGGCAGCAC CGCAAGGTGC GGCTTCACCA GCGGCTGCAG 
CTGTGTGTCT TCCAGCAGGA TGTGCAGCAG GTGCTGGACT GGATTGAAAA CCATGGCGAG 
GCCTTTCTCA GCAAACACAC TGGGGTTGGC AAGTCCTTGC ATCGAGCACG TGCACTACAG 
AAGAGGCATG ATGACTTTGA AGAAGTGGCA CAGAATACAT ACACCAATGC GGACAAGCTC 
CTAGAAGCAG CGGAGCAGCT GGCTCAGACT GGGGAATGTG ACCCGGAGGA GATCTACAAG 
GCAGCCCGGC ACCTGGAGGT GCGCATCCAA GACTTTGTGC GCAGAGTGGA GCAGCGGAAG 
CTGCTTCTGG ACATGTCGGT CTCTTTCCAC ACGCACACCA AAGAGCTGTG GACATGGATG 
GAGGACCTTC AGAAGGAGGT GCTGGAGGAT GTCTGTGCAG ACTCTGTGGA TGCGGTCCAG 
GAGCTGATCA AGCAGTTCCA GCAGCAGCAG ACCGCCACCC TGGATGCCAC TCTCAATGTC 
ATCAAGGAAG GCGAAGACCT TATCCAGCAG CTCAGGTCAG CGCCTCCCTC CCTGGGGGAG 
CCCACCGAGG CCAGGGACTC AGCGATGTCC AACAACAAGA CACCCCACAG TAGCTCTATC 
AGCCATATTG AGTCTGTCCT CCAGCAGCTG GACGATGCTC AGGTACAGAT GGAAGAGCTG 
TTTCACGAGC GGAAGATCAA GCTGGACATC TTCCTACAGC TGCGCATCTT TGAACAGTAC 
ACCATTGAGG TCACTGCAGA GCTGGATGCC TGGAACGAGG ACTTGCTCCG GCAGATGAAT 
GATTTCAACA CAGAGGATCT GACCCTGGCT GAGCAGCGGC TACAGCGCCA CACAGAACGG 
AAGCTTGCCA TGAACAACAT GACGTTCGAG GTCATCCAGC AGGGCCAGGA CCTGCACCAG 
TACATCATGG AAGTGCAAGC GTCAGGAATC GAGTTAATCT GTGAGAAGGA CCTTGACCTG 
GCAGCTCAGG TGCAGGAGCT GCTGGAGTTT CTCCATGAGA AGCAGCACGA GCTGGAGCTC 
AATGCGGAGC AGACGCACAA GCGGCTAGAG CAGTGTCTCC AGCTGAGGCA CCTCCAGGCT 
GAGGTCAAAC AGGTTCTGGG ATGGATCCGC AATGGAGAGT CGATGCTCAA CGCCAGCCTG 
GTCAACGCCA GCTCTTTGTC GGAGGCAGAG CAGCTGCAGC GGGAGCATGA ACAGTTCCAG 
CTAGCCATCG AGTCCCTCTT TCATGCCACT TCCTTGCAGA AGACGCACCA GAGCGCCCTG 
CAGGTACAGC AGAAGGCAGA GGCACTGCTC CAGGCCGGCC ACTACGACGC AGACGCCATC 
CGGGAATGTG CTGAGAAGGT GGCCCTCCAC TGGCAACAGC TCATGCTAAA GATGGAGGAC 
CGGCTGAAGC TTGTCAACGC CTCTGTGGCC TTTTACAAGA CCTCTGAGCA GGTGTGCAGT 
GTTCTGGAGA GCTTGGAGCA GGAGTACCGG AGAGACGAGG ACTGGTGTGG TGGGAGAGAC 
AAGCTGGGGC CGGCGGCAGA GATGGACCAC GTTATTCCAC TCCTCAGCAA GCACCTGGAG 
CAGAAGGAGG CCTTCCTGAA GGCCTGCACG CTGGCAAGGC GGAACGCCGA GGTGTTCCTC 
AAATACATCC ACAGGAACAA TGTGAGCATG CCTAGTGTTG CCAGCCACAC CCGGGGGCCT 
GAACAGCAAG TAAAAGCCAT CTTGAGCGAG CTCTTGCAGA GGGAGAATCG AGTCCTGCAC 
TTCTGGACCT TGAAGAAGCG GCGGTTAGAC CAGTGTCAGC AATATGTGGT GTTTGAGCGC 
AGTGCTAAGC AGGCACTGGA CTGGATCCAA GAAACAGGAG AATATTACCT CTCAACACAT 
ACCTCCACCG GAGAGACCAC GGAGGAGACT CAGGAGCTGC TGAAAGAGTA TGGAGAATTC 
AGGGTGCCTG CCAAGCAAAC GAAGGAGAAG GTGAAACTTC TGATCCAGCT AGCAGACAGC 
TTTGTGGAAA AGGGCCACAT CCACGCCACA GAGATACGGA AATGGGTGAC CACGGTGGAC 
AAGCACTACC GCGATTTCTC CCTGAGGATG GGGAAGTACC GGTACTCGCT GGAGAAGGCG 
CTGGGGGTCA ACACGGAGGA TAACAAGGAC CTAGAGCTGG ACATCATCCC AGCAAGCCTT 
TCGGATCGGG AGGTCAAGCT CCGGGACGCT AACCATGAGA TCAATGAAGA GAAACGGAAG 
TCAGCTCGAA AGAAAGAGTT CATTATGGCT GAACTGCTCC AAACTGAGAA GGCTTATGTA 
AGGGACTTGC ACGAGTGCTT GGAGACCTAC CTGTGGGAGA TGACTAGTGG TGTGGAAGAG 
ATACCCCCCG GCATCCTCAA TAAGGAACAT ATCATCTTCG GCAACATCCA GGAGATCTAT 
GACTTCCATA ACAACATCTT CCTCAAAGAG CTGGAGAAGT ACGAGCAGCT GCCTGAGGAT 
GTGGGACACT GCTTTGTTAC CTGGGCAGAC AAATTTCAGA TGTATGTCAC CTACTGTAAA 
AACAAGCCTG ATTCCAACCA GCTGATCCTG GAGCATGCGG GCACCTTCTT TGATGAGATC 
CAGCAGCGGC ATGGCCTGGC CAACTCCATC TCCTCCTACC TAATTAAGCC TGTCCAGAGG 
GTTACCAAGT ACCAGCTGCT CCTGAAGGAA CTTCTAACTT GCTGTGAAGA AGGGAAAGGA 
GAGCTGAAGG ATGGCCTGGA GGTGATGCTC AGTGTCCCCA AGAAAGCCAA CGATGCCATG 
CATGTCAGCA TGCTGGAAGG GTTCGATGAA AACTTGGATG TACAGGGGGA GTTGATTCTC 
CAGGATGCCT TTCAAGTGTG GGACCCAAAG TCGCTGATCC GGAAGGGGCG GGAGCGCCAT 
CTGTTCCTCT TTGAGATCTC CTTGGTGTTT AGCAAAGAGA TCAAAGACTC TTCAGGACAC 
ACGAAATATG TTTACAAGAA CAAGCTACTG ACCTCAGAGC TGGGTGTGAC CGAGCATGTA 
GAAGGTGACC CCTGCAAATT CGCCTTGTGG TCTGGGCGCA CCCCATCCTC AGACAATAAA 
ACAGTGCTCA AAGCTTCCAA CATCGAAACC AAGCAGGAAT GGATCAAGAA CATTCGAGAA 
GTGATTCAGG AGAGGATCAT TCATCTTAAA GGCGCTTTAA AGGAGCCAAT TCAGCTCCCC 
AAAACACCAG CCAAACTGAG GAACAATAGT AAGAGGGATG GGGTAGAGGA TGGTGACAGC 
CAAGGGGATG GGAGCAGCCA GCCAGATACC ATATCCATTG CCTCCAGGAC CTCTCAGAAC 
ACAGTGGAAA GTGACAAGCT CTCAGGTGGA TGTGAGCTGA CCGTGGTCCT CCAGGACTTC 
AGCGCAGGCC ACAGCAGCGA GCTGAGCATC CAGGTGGGCC AGACGGTGGA GCTGCTGGAA 
CGACCGAGTG AGAGGCCTGG CTGGTGTCTG GTCCGCACCA CAGAGCGGAG CCCCCCACAG 
GAAGGCCTGG TACCCAGCAG CGCCCTGTGC ATCTCACACT CTCGCAGCAG CGTGGAAATG 
GACTGCTTCT TCCCCTTGAA AGATTCATAC TCTCATTCCT CCAGTGAGAA TGGAGGCAAA 
TCCGAGTCCG TGGCCCACCT GCAGTCTCAG CCCTCCCTGA ACTCCATCCA CAGTTCCCCT 
GGTCCCAAGC GCTCCACCAA CACCCTCAAA AAGTGGCTGA CCAGCCCCGT GCGCCGCCTC 
AACAGCGGAA AAGCCGACGG GAACATCAAA AAGCAGAAGA AAGTCCGGGA TGGGCGGAAG 
AGCTTCGATC TGGGATCGCC CAAGCCTGGG GATGAGACGA CACCTCAGGG AGACAGCGCT 
GACGAGAAGA GCAAGAAGGG TTGGGGCGAA GACGAGCCAG ATGAAGAGTC ACACACACCC 
CTCCCTCCGC CCATGAAGAT CTTTGACAAC GACCCCACAC AGGATGAGAT GTCGTCTTTG 
CTAGCAGCCC GGCAGGCTCC CCCTGACGTG CCTACTGCTG CAGACCTTGT CAGTGCAATA 
GAAAAGTTGG TCAAAAATAA GCTGACTCTG GAAGGAGGCT CTTACCGGGG GAGCTTGAAG 
GACCCTACAG GCTGCCTGAA TGAGGGGATG ACCCCTCCCA CACCTCCTAG AAACCTGGAA 
GAAGAACAGA AAGCCAAGGC GCTGAGAGGC AGGATGTTTG TCCTGAATGA GCTGGTACAG 
ACAGAGAAGG ACTACGTCAA GGACCTGGGG ATTGTGGTGG AGGGCTTCAT GAAGAGAATA 
GAAGAGAAGG GGGTCCCTGA GGACATGCGA GGGAAGGAAA AGATCGTGTT TGGAAATATC 
CACCAGATTT ACGACTGGCA TAAAGATTTT TTCCTGGCTG AACTGGAAAA ATGTATCCAA 
GAGCAAGACA GATTGGCCCA GCTCTTCATT AAACACGAGC GGAAGCTGCA CATCTACGTG 
TGGTACTGCC AGAACAAGCC GCGTTCCGAG TACATCGTGG CTGAGTACGA TGCCTATTTT 
GAGGAGGTTA AACAAGAGAT CAATCAAAGG CTGACCCTTA GTGACTTCCT GATCAAGCCC 
ATTCAGAGAA TAACAAAGTA CCAGCTGCTA CTCAAGGACT TCCTGCGATA CAGCGAGAAG 
GCTGGTTTGG AGTGTTCCGA TATTGAGAAA GCAGTGGAGT TAATGTGCCT TGTCCCCAAG 
CGCTGTAATG ACATGATGAA TCTGGGACGC CTGCAGGGCT TCGAGGGCAC CCTGACCGCC 
CAGGGCAAGC TACTGCAGCA GGACACATTC TATGTGATCG AGCTGGATGC CGGCATGCAG 
TCCCGGACCA AGGAGAGGCG TGTGTTTCTC TTTGAGCAGA TTGTCATCTT CAGTGAACTG 
CTCCGAAAGG GGTCCCTCAC CCCAGGCTAC ATGTTCAAAA GAAGCATCAA GATGAATTAC 
TTGGTCCTGG AAGACAATGT GGATGGTGAC CCCTGCAAAT TTGCACTCAT GAACAGGGAG 
ACTTCAGAAA GGGTCATTCT ACAGGCTGCC AACTCAGACA TCCAGCAGGC CTGGGTGCAG 
GACATCAACC AAGTCCTAGA AACACAGAGG GACTTCTTAA ATGCACTACA GTCACCCATT 
GAGTATCAGC GGAAGGAGAG AAGCACAGCT GTGATCCGGT CCCAGCCCCC GAGGGTTCCA 
CAAGCCAGCC CCAGGCCCTA CTCTTCTGGC CCGGTGGGCT CAGAGAAGCC CCCCAAGGGC 
TCCAGCTATA ACCCGCCTCT GCCACCCCTG AAGATATCTA CCTCTAATGG CAGTCCAGGG 
TTCGACTACC ATCAGCCTGG GGACAAGTTT GACGCTAGCA AGCAGAACGA CCTGGGAGGC 
TGCAATGGGA CATCAACCAT GACTGTAATC AAAGATTACT ATGCACTGAA GGAGAATGAA 
ATCTGCGTGA GCCAAGGTGA GGTGGTACAG GTCCTCGCCG TCAACCAGCA GAACATGTGT 
CTGGTGTACC AGCCTGCCAG CGACCATTCC CCGGCAGCAG AGGGCTGGGT CCCAGGCAGC 
ATCCTGGCGC CCCTCGCCAA AGCCACAGCA GCGGCCGAAA GTAGTGACGG GAGCATCAAG 
AAGTCATGCT CTTGGCACAC TCTACGCATG AGAAAGCGGG CAGACGTGGA GAACTCGGGT 
AAAAATGAAG CCACAGGGCC TCGTAAACCC AAGGATATTC TGGGCAACAA AGTCTCTGTT 
AAAGAGACGA ACAGTTCCGA GGAGTCAGAG TGTGATGATC TTGACCCTAA TACTAGTATG 
GAGATCTTGA ATCCAAATTT CATCCAGGAA GTGGCTCCAG AATTCCTCGT GCCCTTAGTA 
GATGTGACCT GTTTGCTTGG AGACACAGTG CTACTGCAGT GTAAAGCCTG TGGGCGGCCA 
AAGCCCAGCA TCACCTGGAA GGGTCCGGAT CAGAACATCC TAGACACTGA CAACAGTTCA 
GCAACATACA CCATCTCCTC CTGTGATTCT GGGGAAAGCA CCCTCAAGAT CTGCAACCTG 
ATGCCCCAGG ATAGTGGGAT TTATACCTGC ATAGCAGCAA ACGACCACGG GACTGCATCC 
ACATCTGCTA CAGTTAAGGT GCAAGGTGTC CCCGCAGCCC CCAACCGCCC AATCGCACAG 
GAGAGGAGCT GCACCTCCGT CATCCTCCGC TGGCTGCCCC CAGCCAGCAC CGGAAACTGC 
ACTATTTCGG GGTACACAGT GGAGTATCGC GAGGAAGGTT CCCAGGTCTG GCAGCAATCC 
GTAGCTTCGA CCTTGGACAC TTACCTGGTC ATTGAAGACC TTAGTCCTGG GTGCCCTTAT 
CAGTTCAGGG TCAGTGCCAG CAACCCCTGG GGAATCAGCC TTCCCAGTGA GCCCTCGGAG 
TTTGTGCGCC TTCCAGAGTA CGATGCAGCC GCCGACGGTG CCACCATTTC TTGGAAGGAA 
AATTTTGATT CTGCTTACAC TGAACTGAAC GAGATTGGCA GAGGCCGTTT CTCTATTGTA 
AAGAAATGCA TTCACAAAGC TACCCGCAAA GATGTTGCTG TGAAATTTGT GAGCAAAAAA 
ATGAAGAAGA AGGAGCAGGC TGCTCACGAG GCCGCCCTGC TCCAGCACCT GCAGCACCCT 
CAGTATGTCA CGCTCCATGA CACCTACGAG TCCCCCACAT CCTACATCCT GATTTTGGAA 
CTGATGGATG ACGGCCGACT CTTGGACTAC CTTATGAATC ATGATGAGCT GATGGAAGAA 
AAGGTAGCTT TCTACATTCG AGACATCATG GAAGCTCTGC AGTACCTGCA CAACTGTAGG 
GTCGCACATT TGGACATAAA GCCCGAAAAC CTGCTCATTG ACCTCCGGAT CCCAGTGCCT 
CGAGTGAAGC TCATCGACTT GGAAGACGCT GTCCAGATCT CAGGCCACTT CCATATCCAC 
CATCTGCTGG GGAACCCTGA GTTCGCTGCC CCCGAAGTGA TCCAAGGCAT CCCTGTCTCC 
CTGGGCACAG ACATCTGGAG CATCGGGGTT CTGACATATG TCATGCTTAG TGGGGTCTCC 
CCCTTCTTAG ATGAGAGCAA AGAGGAAACC TGTATCAACG TGTGCAGGGT GGACTTTAGC 
TTCCCCCACG AGTACTTCTG CGGTGTGAGC AATGCTGCCA GGGATTTCAT CAACGTAATC 
TTACAGGAAG ACTTCCGGAG GCGGCCCACA GCAGCCACGT GCCTGCAGCA TCCCTGGCTA 
CAGCCCCACA ACGGCAGCTA CTCCAAGATC CCCTTGGACA CCTCCCGTCT GGCCTGCTTC 
ATAGAACGCC GCAAGCACCA GAATGATGTG CGGCCCATTC CCAATGTCAA GAGTTACATC 
GTCAATAGGG TGAACCAAGG GACCTCGCTA TCCCACAACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245955.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244257.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X5, mRNA.

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CloneID	OMu75311
Clone ID Related Accession (Same CDS sequence)	XM_011245955.1 , XM_011245955.2
Accession Version	XM_011245955.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCCC CGGAGGGGGC ATCGGAGGAA GGGGGAGCCG CCGACTCGGA CGTGGACGCC 
TTTTTCCGGA CAGGATCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCTATC 
CTAAAGGAGA AAGTGGCCTT TGTGTCTGGG GGGCGTGACA AGCGAGGTGG ACCCATCCTG 
ACCTTCCCTG CCCGAAGCAA TCATGACAGA ATAAGACAGG AAGACCTACG GAAACTTGTG 
ACGTATTTGG CCAGCGTGCC AAGTGAGGAC GTTTGCAAAC GTGGCTTCAC CGTCATCATC 
GACATGCGGG GCTCCAAGTG GGATCTCATC AAGCCCCTCC TCAAGACGCT GCAGGAAGCC 
TTCCCCGCCG AGATCCATGT GGCCCTCATC ATCAAGCCTG ACAACTTCTG GCAGAAGCAG 
AAGACCAACT TTGGCAGCTC CAAATTCATC TTTGAGACAA GCATGGTGTC TGTGGAGGGC 
CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACAGAGGAGT TTGACGGCTC CCTGGATTAC 
AACCATGAGG AGTGGATTGA GTTGCGCCTC TCCCTGGAGG AGTTCTTCAA CAGTGCGGTC 
CACCTGCTCT CACGCCTTGA GGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCTGTG 
GATGTGGAGG GCTCTCGGAG GCTTATCGAC GAGCACACGC AACTTAAGAA AAAGGTGCTG 
AAGGCCCCCG TGGAAGAGCT GGACCGGGAG GGGCAGCGTC TACTGCAGTG CATCCGCTGC 
AGCGATGGCT TCTCAGGGCG CAACTGCATC CCAGGAAGTG CCGACTTCCA GAGCCTTGTA 
CCCAAGATCA CCAGCCTCCT GGACAAGCTG CATTCCACCC GACAACACCT GCACCAAATG 
TGGCACGTGC GAAAGCTCAA GCTGGATCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT 
GCTGAGAAGA TGTTCGACTG GATAAGCCAC AACAAGGAGT TATTTCTTCA GAGCCACACA 
GAGATCGGAG TCAGCTACCA GCATGCACTA GACCTCCAGA CACAGCACAA TCACTTTGCC 
ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTCGC TTCCCGCCTC 
TCAGAGGCCG GCCATTATGC CTCCCAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG 
GAGTGGAAGA GCTTTGCCGC TGCCCTGGAT GAACGCAGTA CAATCCTGGC CATGTCTGCT 
GTGTTCCACC AGAAGGCAGA GCAGTTCCTG TCAGGCGTGG ATGCCTGGTG TAAGATGTGC 
AGCGAAGGCG GCCTTCCTTC TGAGATGCAG GACCTGGAGC TGGCAATCCA CCATCACCAG 
TCCTTATATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGATGG CAAAGCACTA 
CTGGATGTCC TGCAGCGTCC CCTGAGTCCT GGGAACTCTG AGTCCCTCAC AGCCACAGCC 
AACTACTCCA AGGCGGTCCA CCAGGTGCTG GATGTGGTCC ACGAGGTGCT GCACCATCAG 
CGACGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TTCACCAGCG GCTGCAGCTG 
TGTGTCTTCC AGCAGGATGT GCAGCAGGTG CTGGACTGGA TTGAAAACCA TGGCGAGGCC 
TTTCTCAGCA AACACACTGG GGTTGGCAAG TCCTTGCATC GAGCACGTGC ACTACAGAAG 
AGGCATGATG ACTTTGAAGA AGTGGCACAG AATACATACA CCAATGCGGA CAAGCTCCTA 
GAAGCAGCGG AGCAGCTGGC TCAGACTGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA 
GCCCGGCACC TGGAGGTGCG CATCCAAGAC TTTGTGCGCA GAGTGGAGCA GCGGAAGCTG 
CTTCTGGACA TGTCGGTCTC TTTCCACACG CACACCAAAG AGCTGTGGAC ATGGATGGAG 
GACCTTCAGA AGGAGGTGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAG 
CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACCCTGG ATGCCACTCT CAATGTCATC 
AAGGAAGGCG AAGACCTTAT CCAGCAGCTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC 
ACCGAGGCCA GGGACTCAGC GATGTCCAAC AACAAGACAC CCCACAGTAG CTCTATCAGC 
CATATTGAGT CTGTCCTCCA GCAGCTGGAC GATGCTCAGG TACAGATGGA AGAGCTGTTT 
CACGAGCGGA AGATCAAGCT GGACATCTTC CTACAGCTGC GCATCTTTGA ACAGTACACC 
ATTGAGGTCA CTGCAGAGCT GGATGCCTGG AACGAGGACT TGCTCCGGCA GATGAATGAT 
TTCAACACAG AGGATCTGAC CCTGGCTGAG CAGCGGCTAC AGCGCCACAC AGAACGGAAG 
CTTGCCATGA ACAACATGAC GTTCGAGGTC ATCCAGCAGG GCCAGGACCT GCACCAGTAC 
ATCATGGAAG TGCAAGCGTC AGGAATCGAG TTAATCTGTG AGAAGGACCT TGACCTGGCA 
GCTCAGGTGC AGGAGCTGCT GGAGTTTCTC CATGAGAAGC AGCACGAGCT GGAGCTCAAT 
GCGGAGCAGA CGCACAAGCG GCTAGAGCAG TGTCTCCAGC TGAGGCACCT CCAGGCTGAG 
GTCAAACAGG TTCTGGGATG GATCCGCAAT GGAGAGTCGA TGCTCAACGC CAGCCTGGTC 
AACGCCAGCT CTTTGTCGGA GGCAGAGCAG CTGCAGCGGG AGCATGAACA GTTCCAGCTA 
GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CGCACCAGAG CGCCCTGCAG 
GTACAGCAGA AGGCAGAGGC ACTGCTCCAG GCCGGCCACT ACGACGCAGA CGCCATCCGG 
GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAACAGCTCA TGCTAAAGAT GGAGGACCGG 
CTGAAGCTTG TCAACGCCTC TGTGGCCTTT TACAAGACCT CTGAGCAGGT GTGCAGTGTT 
CTGGAGAGCT TGGAGCAGGA GTACCGGAGA GACGAGGACT GGTGTGGTGG GAGAGACAAG 
CTGGGGCCGG CGGCAGAGAT GGACCACGTT ATTCCACTCC TCAGCAAGCA CCTGGAGCAG 
AAGGAGGCCT TCCTGAAGGC CTGCACGCTG GCAAGGCGGA ACGCCGAGGT GTTCCTCAAA 
TACATCCACA GGAACAATGT GAGCATGCCT AGTGTTGCCA GCCACACCCG GGGGCCTGAA 
CAGCAAGTAA AAGCCATCTT GAGCGAGCTC TTGCAGAGGG AGAATCGAGT CCTGCACTTC 
TGGACCTTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ATGTGGTGTT TGAGCGCAGT 
GCTAAGCAGG CACTGGACTG GATCCAAGAA ACAGGAGAAT ATTACCTCTC AACACATACC 
TCCACCGGAG AGACCACGGA GGAGACTCAG GAGCTGCTGA AAGAGTATGG AGAATTCAGG 
GTGCCTGCCA AGCAAACGAA GGAGAAGGTG AAACTTCTGA TCCAGCTAGC AGACAGCTTT 
GTGGAAAAGG GCCACATCCA CGCCACAGAG ATACGGAAAT GGGTGACCAC GGTGGACAAG 
CACTACCGCG ATTTCTCCCT GAGGATGGGG AAGTACCGGT ACTCGCTGGA GAAGGCGCTG 
GGGGTCAACA CGGAGGATAA CAAGGACCTA GAGCTGGACA TCATCCCAGC AAGCCTTTCG 
GATCGGGAGG TCAAGCTCCG GGACGCTAAC CATGAGATCA ATGAAGAGAA ACGGAAGTCA 
GCTCGAAAGA AAGAGTTCAT TATGGCTGAA CTGCTCCAAA CTGAGAAGGC TTATGTAAGG 
GACTTGCACG AGTGCTTGGA GACCTACCTG TGGGAGATGA CTAGTGGTGT GGAAGAGATA 
CCCCCCGGCA TCCTCAATAA GGAACATATC ATCTTCGGCA ACATCCAGGA GATCTATGAC 
TTCCATAACA ACATCTTCCT CAAAGAGCTG GAGAAGTACG AGCAGCTGCC TGAGGATGTG 
GGACACTGCT TTGTTACCTG GGCAGACAAA TTTCAGATGT ATGTCACCTA CTGTAAAAAC 
AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCGGGCA CCTTCTTTGA TGAGATCCAG 
CAGCGGCATG GCCTGGCCAA CTCCATCTCC TCCTACCTAA TTAAGCCTGT CCAGAGGGTT 
ACCAAGTACC AGCTGCTCCT GAAGGAACTT CTAACTTGCT GTGAAGAAGG GAAAGGAGAG 
CTGAAGGATG GCCTGGAGGT GATGCTCAGT GTCCCCAAGA AAGCCAACGA TGCCATGCAT 
GTCAGCATGC TGGAAGGGTT CGATGAAAAC TTGGATGTAC AGGGGGAGTT GATTCTCCAG 
GATGCCTTTC AAGTGTGGGA CCCAAAGTCG CTGATCCGGA AGGGGCGGGA GCGCCATCTG 
TTCCTCTTTG AGATCTCCTT GGTGTTTAGC AAAGAGATCA AAGACTCTTC AGGACACACG 
AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA GCATGTAGAA 
GGTGACCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA 
GTGCTCAAAG CTTCCAACAT CGAAACCAAG CAGGAATGGA TCAAGAACAT TCGAGAAGTG 
ATTCAGGAGA GGATCATTCA TCTTAAAGGC GCTTTAAAGG AGCCAATTCA GCTCCCCAAA 
ACACCAGCCA AACTGAGGAA CAATAGTAAG AGGGATGGGG TAGAGGATGG TGACAGCCAA 
GGGGATGGGA GCAGCCAGCC AGATACCATA TCCATTGCCT CCAGGACCTC TCAGAACACA 
GTGGAAAGTG ACAAGCTCTC AGGTGGATGT GAGCTGACCG TGGTCCTCCA GGACTTCAGC 
GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGCCAGA CGGTGGAGCT GCTGGAACGA 
CCGAGTGAGA GGCCTGGCTG GTGTCTGGTC CGCACCACAG AGCGGAGCCC CCCACAGGAA 
GGCCTGGTAC CCAGCAGCGC CCTGTGCATC TCACACTCTC GCAGCAGCGT GGAAATGGAC 
TGCTTCTTCC CCTTGAAAGA TTCATACTCT CATTCCTCCA GTGAGAATGG AGGCAAATCC 
GAGTCCGTGG CCCACCTGCA GTCTCAGCCC TCCCTGAACT CCATCCACAG TTCCCCTGGT 
CCCAAGCGCT CCACCAACAC CCTCAAAAAG TGGCTGACCA GCCCCGTGCG CCGCCTCAAC 
AGCGGAAAAG CCGACGGGAA CATCAAAAAG CAGAAGAAAG TCCGGGATGG GCGGAAGAGC 
TTCGATCTGG GATCGCCCAA GCCTGGGGAT GAGACGACAC CTCAGGGAGA CAGCGCTGAC 
GAGAAGAGCA AGAAGGGTTG GGGCGAAGAC GAGCCAGATG AAGAGTCACA CACACCCCTC 
CCTCCGCCCA TGAAGATCTT TGACAACGAC CCCACACAGG ATGAGATGTC GTCTTTGCTA 
GCAGCCCGGC AGGCTCCCCC TGACGTGCCT ACTGCTGCAG ACCTTGTCAG TGCAATAGAA 
AAGTTGGTCA AAAATAAGCT GACTCTGGAA GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC 
CCTACAGGCT GCCTGAATGA GGGGATGACC CCTCCCACAC CTCCTAGAAA CCTGGAAGAA 
GAACAGAAAG CCAAGGCGCT GAGAGGCAGG ATGTTTGTCC TGAATGAGCT GGTACAGACA 
GAGAAGGACT ACGTCAAGGA CCTGGGGATT GTGGTGGAGG GCTTCATGAA GAGAATAGAA 
GAGAAGGGGG TCCCTGAGGA CATGCGAGGG AAGGAAAAGA TCGTGTTTGG AAATATCCAC 
CAGATTTACG ACTGGCATAA AGATTTTTTC CTGGCTGAAC TGGAAAAATG TATCCAAGAG 
CAAGACAGAT TGGCCCAGCT CTTCATTAAA CACGAGCGGA AGCTGCACAT CTACGTGTGG 
TACTGCCAGA ACAAGCCGCG TTCCGAGTAC ATCGTGGCTG AGTACGATGC CTATTTTGAG 
GAGGTTAAAC AAGAGATCAA TCAAAGGCTG ACCCTTAGTG ACTTCCTGAT CAAGCCCATT 
CAGAGAATAA CAAAGTACCA GCTGCTACTC AAGGACTTCC TGCGATACAG CGAGAAGGCT 
GGTTTGGAGT GTTCCGATAT TGAGAAAGCA GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC 
TGTAATGACA TGATGAATCT GGGACGCCTG CAGGGCTTCG AGGGCACCCT GACCGCCCAG 
GGCAAGCTAC TGCAGCAGGA CACATTCTAT GTGATCGAGC TGGATGCCGG CATGCAGTCC 
CGGACCAAGG AGAGGCGTGT GTTTCTCTTT GAGCAGATTG TCATCTTCAG TGAACTGCTC 
CGAAAGGGGT CCCTCACCCC AGGCTACATG TTCAAAAGAA GCATCAAGAT GAATTACTTG 
GTCCTGGAAG ACAATGTGGA TGGTGACCCC TGCAAATTTG CACTCATGAA CAGGGAGACT 
TCAGAAAGGG TCATTCTACA GGCTGCCAAC TCAGACATCC AGCAGGCCTG GGTGCAGGAC 
ATCAACCAAG TCCTAGAAAC ACAGAGGGAC TTCTTAAATG CACTACAGTC ACCCATTGAG 
TATCAGCGGA AGGAGAGAAG CACAGCTGTG ATCCGGTCCC AGCCCCCGAG GGTTCCACAA 
GCCAGCCCCA GGCCCTACTC TTCTGGCCCG GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC 
AGCTATAACC CGCCTCTGCC ACCCCTGAAG ATATCTACCT CTAATGGCAG TCCAGGGTTC 
GACTACCATC AGCCTGGGGA CAAGTTTGAC GCTAGCAAGC AGAACGACCT GGGAGGCTGC 
AATGGGACAT CAACCATGAC TGTAATCAAA GATTACTATG CACTGAAGGA GAATGAAATC 
TGCGTGAGCC AAGGTGAGGT GGTACAGGTC CTCGCCGTCA ACCAGCAGAA CATGTGTCTG 
GTGTACCAGC CTGCCAGCGA CCATTCCCCG GCAGCAGAGG GCTGGGTCCC AGGCAGCATC 
CTGGCGCCCC TCGCCAAAGC CACAGCAGCG GCCGAAAGTA GTGACGGGAG CATCAAGAAG 
TCATGCTCTT GGCACACTCT ACGCATGAGA AAGCGGGCAG ACGTGGAGAA CTCGGGTAAA 
AATGAAGCCA CAGGGCCTCG TAAACCCAAG GATATTCTGG GCAACAAAGT CTCTGTTAAA 
GAGACGAACA GTTCCGAGGA GTCAGAGTGT GATGATCTTG ACCCTAATAC TAGTATGGAG 
ATCTTGAATC CAAATTTCAT CCAGGAAGTG GCTCCAGAAT TCCTCGTGCC CTTAGTAGAT 
GTGACCTGTT TGCTTGGAGA CACAGTGCTA CTGCAGTGTA AAGCCTGTGG GCGGCCAAAG 
CCCAGCATCA CCTGGAAGGG TCCGGATCAG AACATCCTAG ACACTGACAA CAGTTCAGCA 
ACATACACCA TCTCCTCCTG TGATTCTGGG GAAAGCACCC TCAAGATCTG CAACCTGATG 
CCCCAGGATA GTGGGATTTA TACCTGCATA GCAGCAAACG ACCACGGGAC TGCATCCACA 
TCTGCTACAG TTAAGGTGCA AGGTGTCCCC GCAGCCCCCA ACCGCCCAAT CGCACAGGAG 
AGGAGCTGCA CCTCCGTCAT CCTCCGCTGG CTGCCCCCAG CCAGCACCGG AAACTGCACT 
ATTTCGGGGT ACACAGTGGA GTATCGCGAG GAAGGTTCCC AGGTCTGGCA GCAATCCGTA 
GCTTCGACCT TGGACACTTA CCTGGTCATT GAAGACCTTA GTCCTGGGTG CCCTTATCAG 
TTCAGGGTCA GTGCCAGCAA CCCCTGGGGA ATCAGCCTTC CCAGTGAGCC CTCGGAGTTT 
GTGCGCCTTC CAGAGTACGA TGCAGCCGCC GACGGTGCCA CCATTTCTTG GAAGGAAAAT 
TTTGATTCTG CTTACACTGA ACTGAACGAG ATTGGCAGAG GCCGTTTCTC TATTGTAAAG 
AAATGCATTC ACAAAGCTAC CCGCAAAGAT GTTGCTGTGA AATTTGTGAG CAAAAAAATG 
AAGAAGAAGG AGCAGGCTGC TCACGAGGCC GCCCTGCTCC AGCACCTGCA GCACCCTCAG 
TATGTCACGC TCCATGACAC CTACGAGTCC CCCACATCCT ACATCCTGAT TTTGGAACTG 
ATGGATGACG GCCGACTCTT GGACTACCTT ATGAATCATG ATGAGCTGAT GGAAGAAAAG 
GTAGCTTTCT ACATTCGAGA CATCATGGAA GCTCTGCAGT ACCTGCACAA CTGTAGGGTC 
GCACATTTGG ACATAAAGCC CGAAAACCTG CTCATTGACC TCCGGATCCC AGTGCCTCGA 
GTGAAGCTCA TCGACTTGGA AGACGCTGTC CAGATCTCAG GCCACTTCCA TATCCACCAT 
CTGCTGGGGA ACCCTGAGTT CGCTGCCCCC GAAGTGATCC AAGGCATCCC TGTCTCCCTG 
GGCACAGACA TCTGGAGCAT CGGGGTTCTG ACATATGTCA TGCTTAGTGG GGTCTCCCCC 
TTCTTAGATG AGAGCAAAGA GGAAACCTGT ATCAACGTGT GCAGGGTGGA CTTTAGCTTC 
CCCCACGAGT ACTTCTGCGG TGTGAGCAAT GCTGCCAGGG ATTTCATCAA CGTAATCTTA 
CAGGAAGACT TCCGGAGGCG GCCCACAGCA GCCACGTGCC TGCAGCATCC CTGGCTACAG 
CCCCACAACG GCAGCTACTC CAAGATCCCC TTGGACACCT CCCGTCTGGC CTGCTTCATA 
GAACGCCGCA AGCACCAGAA TGATGTGCGG CCCATTCCCA ATGTCAAGAG TTACATCGTC 
AATAGGGTGA ACCAAGGGAC CTCGCTATCC CACAACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244257.1
CDS	113..9094
Translation	MNPPEGASEEGGAADSDVDAFFRTGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245955.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245955.1

ORF Insert Sequence:

ATGAACCCCC CGGAGGGGGC ATCGGAGGAA GGGGGAGCCG CCGACTCGGA CGTGGACGCC 
TTTTTCCGGA CAGGATCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCTATC 
CTAAAGGAGA AAGTGGCCTT TGTGTCTGGG GGGCGTGACA AGCGAGGTGG ACCCATCCTG 
ACCTTCCCTG CCCGAAGCAA TCATGACAGA ATAAGACAGG AAGACCTACG GAAACTTGTG 
ACGTATTTGG CCAGCGTGCC AAGTGAGGAC GTTTGCAAAC GTGGCTTCAC CGTCATCATC 
GACATGCGGG GCTCCAAGTG GGATCTCATC AAGCCCCTCC TCAAGACGCT GCAGGAAGCC 
TTCCCCGCCG AGATCCATGT GGCCCTCATC ATCAAGCCTG ACAACTTCTG GCAGAAGCAG 
AAGACCAACT TTGGCAGCTC CAAATTCATC TTTGAGACAA GCATGGTGTC TGTGGAGGGC 
CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACAGAGGAGT TTGACGGCTC CCTGGATTAC 
AACCATGAGG AGTGGATTGA GTTGCGCCTC TCCCTGGAGG AGTTCTTCAA CAGTGCGGTC 
CACCTGCTCT CACGCCTTGA GGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCTGTG 
GATGTGGAGG GCTCTCGGAG GCTTATCGAC GAGCACACGC AACTTAAGAA AAAGGTGCTG 
AAGGCCCCCG TGGAAGAGCT GGACCGGGAG GGGCAGCGTC TACTGCAGTG CATCCGCTGC 
AGCGATGGCT TCTCAGGGCG CAACTGCATC CCAGGAAGTG CCGACTTCCA GAGCCTTGTA 
CCCAAGATCA CCAGCCTCCT GGACAAGCTG CATTCCACCC GACAACACCT GCACCAAATG 
TGGCACGTGC GAAAGCTCAA GCTGGATCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT 
GCTGAGAAGA TGTTCGACTG GATAAGCCAC AACAAGGAGT TATTTCTTCA GAGCCACACA 
GAGATCGGAG TCAGCTACCA GCATGCACTA GACCTCCAGA CACAGCACAA TCACTTTGCC 
ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTCGC TTCCCGCCTC 
TCAGAGGCCG GCCATTATGC CTCCCAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG 
GAGTGGAAGA GCTTTGCCGC TGCCCTGGAT GAACGCAGTA CAATCCTGGC CATGTCTGCT 
GTGTTCCACC AGAAGGCAGA GCAGTTCCTG TCAGGCGTGG ATGCCTGGTG TAAGATGTGC 
AGCGAAGGCG GCCTTCCTTC TGAGATGCAG GACCTGGAGC TGGCAATCCA CCATCACCAG 
TCCTTATATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGATGG CAAAGCACTA 
CTGGATGTCC TGCAGCGTCC CCTGAGTCCT GGGAACTCTG AGTCCCTCAC AGCCACAGCC 
AACTACTCCA AGGCGGTCCA CCAGGTGCTG GATGTGGTCC ACGAGGTGCT GCACCATCAG 
CGACGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TTCACCAGCG GCTGCAGCTG 
TGTGTCTTCC AGCAGGATGT GCAGCAGGTG CTGGACTGGA TTGAAAACCA TGGCGAGGCC 
TTTCTCAGCA AACACACTGG GGTTGGCAAG TCCTTGCATC GAGCACGTGC ACTACAGAAG 
AGGCATGATG ACTTTGAAGA AGTGGCACAG AATACATACA CCAATGCGGA CAAGCTCCTA 
GAAGCAGCGG AGCAGCTGGC TCAGACTGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA 
GCCCGGCACC TGGAGGTGCG CATCCAAGAC TTTGTGCGCA GAGTGGAGCA GCGGAAGCTG 
CTTCTGGACA TGTCGGTCTC TTTCCACACG CACACCAAAG AGCTGTGGAC ATGGATGGAG 
GACCTTCAGA AGGAGGTGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAG 
CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACCCTGG ATGCCACTCT CAATGTCATC 
AAGGAAGGCG AAGACCTTAT CCAGCAGCTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC 
ACCGAGGCCA GGGACTCAGC GATGTCCAAC AACAAGACAC CCCACAGTAG CTCTATCAGC 
CATATTGAGT CTGTCCTCCA GCAGCTGGAC GATGCTCAGG TACAGATGGA AGAGCTGTTT 
CACGAGCGGA AGATCAAGCT GGACATCTTC CTACAGCTGC GCATCTTTGA ACAGTACACC 
ATTGAGGTCA CTGCAGAGCT GGATGCCTGG AACGAGGACT TGCTCCGGCA GATGAATGAT 
TTCAACACAG AGGATCTGAC CCTGGCTGAG CAGCGGCTAC AGCGCCACAC AGAACGGAAG 
CTTGCCATGA ACAACATGAC GTTCGAGGTC ATCCAGCAGG GCCAGGACCT GCACCAGTAC 
ATCATGGAAG TGCAAGCGTC AGGAATCGAG TTAATCTGTG AGAAGGACCT TGACCTGGCA 
GCTCAGGTGC AGGAGCTGCT GGAGTTTCTC CATGAGAAGC AGCACGAGCT GGAGCTCAAT 
GCGGAGCAGA CGCACAAGCG GCTAGAGCAG TGTCTCCAGC TGAGGCACCT CCAGGCTGAG 
GTCAAACAGG TTCTGGGATG GATCCGCAAT GGAGAGTCGA TGCTCAACGC CAGCCTGGTC 
AACGCCAGCT CTTTGTCGGA GGCAGAGCAG CTGCAGCGGG AGCATGAACA GTTCCAGCTA 
GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CGCACCAGAG CGCCCTGCAG 
GTACAGCAGA AGGCAGAGGC ACTGCTCCAG GCCGGCCACT ACGACGCAGA CGCCATCCGG 
GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAACAGCTCA TGCTAAAGAT GGAGGACCGG 
CTGAAGCTTG TCAACGCCTC TGTGGCCTTT TACAAGACCT CTGAGCAGGT GTGCAGTGTT 
CTGGAGAGCT TGGAGCAGGA GTACCGGAGA GACGAGGACT GGTGTGGTGG GAGAGACAAG 
CTGGGGCCGG CGGCAGAGAT GGACCACGTT ATTCCACTCC TCAGCAAGCA CCTGGAGCAG 
AAGGAGGCCT TCCTGAAGGC CTGCACGCTG GCAAGGCGGA ACGCCGAGGT GTTCCTCAAA 
TACATCCACA GGAACAATGT GAGCATGCCT AGTGTTGCCA GCCACACCCG GGGGCCTGAA 
CAGCAAGTAA AAGCCATCTT GAGCGAGCTC TTGCAGAGGG AGAATCGAGT CCTGCACTTC 
TGGACCTTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ATGTGGTGTT TGAGCGCAGT 
GCTAAGCAGG CACTGGACTG GATCCAAGAA ACAGGAGAAT ATTACCTCTC AACACATACC 
TCCACCGGAG AGACCACGGA GGAGACTCAG GAGCTGCTGA AAGAGTATGG AGAATTCAGG 
GTGCCTGCCA AGCAAACGAA GGAGAAGGTG AAACTTCTGA TCCAGCTAGC AGACAGCTTT 
GTGGAAAAGG GCCACATCCA CGCCACAGAG ATACGGAAAT GGGTGACCAC GGTGGACAAG 
CACTACCGCG ATTTCTCCCT GAGGATGGGG AAGTACCGGT ACTCGCTGGA GAAGGCGCTG 
GGGGTCAACA CGGAGGATAA CAAGGACCTA GAGCTGGACA TCATCCCAGC AAGCCTTTCG 
GATCGGGAGG TCAAGCTCCG GGACGCTAAC CATGAGATCA ATGAAGAGAA ACGGAAGTCA 
GCTCGAAAGA AAGAGTTCAT TATGGCTGAA CTGCTCCAAA CTGAGAAGGC TTATGTAAGG 
GACTTGCACG AGTGCTTGGA GACCTACCTG TGGGAGATGA CTAGTGGTGT GGAAGAGATA 
CCCCCCGGCA TCCTCAATAA GGAACATATC ATCTTCGGCA ACATCCAGGA GATCTATGAC 
TTCCATAACA ACATCTTCCT CAAAGAGCTG GAGAAGTACG AGCAGCTGCC TGAGGATGTG 
GGACACTGCT TTGTTACCTG GGCAGACAAA TTTCAGATGT ATGTCACCTA CTGTAAAAAC 
AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCGGGCA CCTTCTTTGA TGAGATCCAG 
CAGCGGCATG GCCTGGCCAA CTCCATCTCC TCCTACCTAA TTAAGCCTGT CCAGAGGGTT 
ACCAAGTACC AGCTGCTCCT GAAGGAACTT CTAACTTGCT GTGAAGAAGG GAAAGGAGAG 
CTGAAGGATG GCCTGGAGGT GATGCTCAGT GTCCCCAAGA AAGCCAACGA TGCCATGCAT 
GTCAGCATGC TGGAAGGGTT CGATGAAAAC TTGGATGTAC AGGGGGAGTT GATTCTCCAG 
GATGCCTTTC AAGTGTGGGA CCCAAAGTCG CTGATCCGGA AGGGGCGGGA GCGCCATCTG 
TTCCTCTTTG AGATCTCCTT GGTGTTTAGC AAAGAGATCA AAGACTCTTC AGGACACACG 
AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA GCATGTAGAA 
GGTGACCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA 
GTGCTCAAAG CTTCCAACAT CGAAACCAAG CAGGAATGGA TCAAGAACAT TCGAGAAGTG 
ATTCAGGAGA GGATCATTCA TCTTAAAGGC GCTTTAAAGG AGCCAATTCA GCTCCCCAAA 
ACACCAGCCA AACTGAGGAA CAATAGTAAG AGGGATGGGG TAGAGGATGG TGACAGCCAA 
GGGGATGGGA GCAGCCAGCC AGATACCATA TCCATTGCCT CCAGGACCTC TCAGAACACA 
GTGGAAAGTG ACAAGCTCTC AGGTGGATGT GAGCTGACCG TGGTCCTCCA GGACTTCAGC 
GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGCCAGA CGGTGGAGCT GCTGGAACGA 
CCGAGTGAGA GGCCTGGCTG GTGTCTGGTC CGCACCACAG AGCGGAGCCC CCCACAGGAA 
GGCCTGGTAC CCAGCAGCGC CCTGTGCATC TCACACTCTC GCAGCAGCGT GGAAATGGAC 
TGCTTCTTCC CCTTGAAAGA TTCATACTCT CATTCCTCCA GTGAGAATGG AGGCAAATCC 
GAGTCCGTGG CCCACCTGCA GTCTCAGCCC TCCCTGAACT CCATCCACAG TTCCCCTGGT 
CCCAAGCGCT CCACCAACAC CCTCAAAAAG TGGCTGACCA GCCCCGTGCG CCGCCTCAAC 
AGCGGAAAAG CCGACGGGAA CATCAAAAAG CAGAAGAAAG TCCGGGATGG GCGGAAGAGC 
TTCGATCTGG GATCGCCCAA GCCTGGGGAT GAGACGACAC CTCAGGGAGA CAGCGCTGAC 
GAGAAGAGCA AGAAGGGTTG GGGCGAAGAC GAGCCAGATG AAGAGTCACA CACACCCCTC 
CCTCCGCCCA TGAAGATCTT TGACAACGAC CCCACACAGG ATGAGATGTC GTCTTTGCTA 
GCAGCCCGGC AGGCTCCCCC TGACGTGCCT ACTGCTGCAG ACCTTGTCAG TGCAATAGAA 
AAGTTGGTCA AAAATAAGCT GACTCTGGAA GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC 
CCTACAGGCT GCCTGAATGA GGGGATGACC CCTCCCACAC CTCCTAGAAA CCTGGAAGAA 
GAACAGAAAG CCAAGGCGCT GAGAGGCAGG ATGTTTGTCC TGAATGAGCT GGTACAGACA 
GAGAAGGACT ACGTCAAGGA CCTGGGGATT GTGGTGGAGG GCTTCATGAA GAGAATAGAA 
GAGAAGGGGG TCCCTGAGGA CATGCGAGGG AAGGAAAAGA TCGTGTTTGG AAATATCCAC 
CAGATTTACG ACTGGCATAA AGATTTTTTC CTGGCTGAAC TGGAAAAATG TATCCAAGAG 
CAAGACAGAT TGGCCCAGCT CTTCATTAAA CACGAGCGGA AGCTGCACAT CTACGTGTGG 
TACTGCCAGA ACAAGCCGCG TTCCGAGTAC ATCGTGGCTG AGTACGATGC CTATTTTGAG 
GAGGTTAAAC AAGAGATCAA TCAAAGGCTG ACCCTTAGTG ACTTCCTGAT CAAGCCCATT 
CAGAGAATAA CAAAGTACCA GCTGCTACTC AAGGACTTCC TGCGATACAG CGAGAAGGCT 
GGTTTGGAGT GTTCCGATAT TGAGAAAGCA GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC 
TGTAATGACA TGATGAATCT GGGACGCCTG CAGGGCTTCG AGGGCACCCT GACCGCCCAG 
GGCAAGCTAC TGCAGCAGGA CACATTCTAT GTGATCGAGC TGGATGCCGG CATGCAGTCC 
CGGACCAAGG AGAGGCGTGT GTTTCTCTTT GAGCAGATTG TCATCTTCAG TGAACTGCTC 
CGAAAGGGGT CCCTCACCCC AGGCTACATG TTCAAAAGAA GCATCAAGAT GAATTACTTG 
GTCCTGGAAG ACAATGTGGA TGGTGACCCC TGCAAATTTG CACTCATGAA CAGGGAGACT 
TCAGAAAGGG TCATTCTACA GGCTGCCAAC TCAGACATCC AGCAGGCCTG GGTGCAGGAC 
ATCAACCAAG TCCTAGAAAC ACAGAGGGAC TTCTTAAATG CACTACAGTC ACCCATTGAG 
TATCAGCGGA AGGAGAGAAG CACAGCTGTG ATCCGGTCCC AGCCCCCGAG GGTTCCACAA 
GCCAGCCCCA GGCCCTACTC TTCTGGCCCG GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC 
AGCTATAACC CGCCTCTGCC ACCCCTGAAG ATATCTACCT CTAATGGCAG TCCAGGGTTC 
GACTACCATC AGCCTGGGGA CAAGTTTGAC GCTAGCAAGC AGAACGACCT GGGAGGCTGC 
AATGGGACAT CAACCATGAC TGTAATCAAA GATTACTATG CACTGAAGGA GAATGAAATC 
TGCGTGAGCC AAGGTGAGGT GGTACAGGTC CTCGCCGTCA ACCAGCAGAA CATGTGTCTG 
GTGTACCAGC CTGCCAGCGA CCATTCCCCG GCAGCAGAGG GCTGGGTCCC AGGCAGCATC 
CTGGCGCCCC TCGCCAAAGC CACAGCAGCG GCCGAAAGTA GTGACGGGAG CATCAAGAAG 
TCATGCTCTT GGCACACTCT ACGCATGAGA AAGCGGGCAG ACGTGGAGAA CTCGGGTAAA 
AATGAAGCCA CAGGGCCTCG TAAACCCAAG GATATTCTGG GCAACAAAGT CTCTGTTAAA 
GAGACGAACA GTTCCGAGGA GTCAGAGTGT GATGATCTTG ACCCTAATAC TAGTATGGAG 
ATCTTGAATC CAAATTTCAT CCAGGAAGTG GCTCCAGAAT TCCTCGTGCC CTTAGTAGAT 
GTGACCTGTT TGCTTGGAGA CACAGTGCTA CTGCAGTGTA AAGCCTGTGG GCGGCCAAAG 
CCCAGCATCA CCTGGAAGGG TCCGGATCAG AACATCCTAG ACACTGACAA CAGTTCAGCA 
ACATACACCA TCTCCTCCTG TGATTCTGGG GAAAGCACCC TCAAGATCTG CAACCTGATG 
CCCCAGGATA GTGGGATTTA TACCTGCATA GCAGCAAACG ACCACGGGAC TGCATCCACA 
TCTGCTACAG TTAAGGTGCA AGGTGTCCCC GCAGCCCCCA ACCGCCCAAT CGCACAGGAG 
AGGAGCTGCA CCTCCGTCAT CCTCCGCTGG CTGCCCCCAG CCAGCACCGG AAACTGCACT 
ATTTCGGGGT ACACAGTGGA GTATCGCGAG GAAGGTTCCC AGGTCTGGCA GCAATCCGTA 
GCTTCGACCT TGGACACTTA CCTGGTCATT GAAGACCTTA GTCCTGGGTG CCCTTATCAG 
TTCAGGGTCA GTGCCAGCAA CCCCTGGGGA ATCAGCCTTC CCAGTGAGCC CTCGGAGTTT 
GTGCGCCTTC CAGAGTACGA TGCAGCCGCC GACGGTGCCA CCATTTCTTG GAAGGAAAAT 
TTTGATTCTG CTTACACTGA ACTGAACGAG ATTGGCAGAG GCCGTTTCTC TATTGTAAAG 
AAATGCATTC ACAAAGCTAC CCGCAAAGAT GTTGCTGTGA AATTTGTGAG CAAAAAAATG 
AAGAAGAAGG AGCAGGCTGC TCACGAGGCC GCCCTGCTCC AGCACCTGCA GCACCCTCAG 
TATGTCACGC TCCATGACAC CTACGAGTCC CCCACATCCT ACATCCTGAT TTTGGAACTG 
ATGGATGACG GCCGACTCTT GGACTACCTT ATGAATCATG ATGAGCTGAT GGAAGAAAAG 
GTAGCTTTCT ACATTCGAGA CATCATGGAA GCTCTGCAGT ACCTGCACAA CTGTAGGGTC 
GCACATTTGG ACATAAAGCC CGAAAACCTG CTCATTGACC TCCGGATCCC AGTGCCTCGA 
GTGAAGCTCA TCGACTTGGA AGACGCTGTC CAGATCTCAG GCCACTTCCA TATCCACCAT 
CTGCTGGGGA ACCCTGAGTT CGCTGCCCCC GAAGTGATCC AAGGCATCCC TGTCTCCCTG 
GGCACAGACA TCTGGAGCAT CGGGGTTCTG ACATATGTCA TGCTTAGTGG GGTCTCCCCC 
TTCTTAGATG AGAGCAAAGA GGAAACCTGT ATCAACGTGT GCAGGGTGGA CTTTAGCTTC 
CCCCACGAGT ACTTCTGCGG TGTGAGCAAT GCTGCCAGGG ATTTCATCAA CGTAATCTTA 
CAGGAAGACT TCCGGAGGCG GCCCACAGCA GCCACGTGCC TGCAGCATCC CTGGCTACAG 
CCCCACAACG GCAGCTACTC CAAGATCCCC TTGGACACCT CCCGTCTGGC CTGCTTCATA 
GAACGCCGCA AGCACCAGAA TGATGTGCGG CCCATTCCCA ATGTCAAGAG TTACATCGTC 
AATAGGGTGA ACCAAGGGAC CTCGCTATCC CACAACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245955.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244257.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X5, mRNA.

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CloneID	OMu75311
Clone ID Related Accession (Same CDS sequence)	XM_011245955.1 , XM_011245955.2
Accession Version	XM_011245955.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCCC CGGAGGGGGC ATCGGAGGAA GGGGGAGCCG CCGACTCGGA CGTGGACGCC 
TTTTTCCGGA CAGGATCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCTATC 
CTAAAGGAGA AAGTGGCCTT TGTGTCTGGG GGGCGTGACA AGCGAGGTGG ACCCATCCTG 
ACCTTCCCTG CCCGAAGCAA TCATGACAGA ATAAGACAGG AAGACCTACG GAAACTTGTG 
ACGTATTTGG CCAGCGTGCC AAGTGAGGAC GTTTGCAAAC GTGGCTTCAC CGTCATCATC 
GACATGCGGG GCTCCAAGTG GGATCTCATC AAGCCCCTCC TCAAGACGCT GCAGGAAGCC 
TTCCCCGCCG AGATCCATGT GGCCCTCATC ATCAAGCCTG ACAACTTCTG GCAGAAGCAG 
AAGACCAACT TTGGCAGCTC CAAATTCATC TTTGAGACAA GCATGGTGTC TGTGGAGGGC 
CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACAGAGGAGT TTGACGGCTC CCTGGATTAC 
AACCATGAGG AGTGGATTGA GTTGCGCCTC TCCCTGGAGG AGTTCTTCAA CAGTGCGGTC 
CACCTGCTCT CACGCCTTGA GGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCTGTG 
GATGTGGAGG GCTCTCGGAG GCTTATCGAC GAGCACACGC AACTTAAGAA AAAGGTGCTG 
AAGGCCCCCG TGGAAGAGCT GGACCGGGAG GGGCAGCGTC TACTGCAGTG CATCCGCTGC 
AGCGATGGCT TCTCAGGGCG CAACTGCATC CCAGGAAGTG CCGACTTCCA GAGCCTTGTA 
CCCAAGATCA CCAGCCTCCT GGACAAGCTG CATTCCACCC GACAACACCT GCACCAAATG 
TGGCACGTGC GAAAGCTCAA GCTGGATCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT 
GCTGAGAAGA TGTTCGACTG GATAAGCCAC AACAAGGAGT TATTTCTTCA GAGCCACACA 
GAGATCGGAG TCAGCTACCA GCATGCACTA GACCTCCAGA CACAGCACAA TCACTTTGCC 
ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTCGC TTCCCGCCTC 
TCAGAGGCCG GCCATTATGC CTCCCAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG 
GAGTGGAAGA GCTTTGCCGC TGCCCTGGAT GAACGCAGTA CAATCCTGGC CATGTCTGCT 
GTGTTCCACC AGAAGGCAGA GCAGTTCCTG TCAGGCGTGG ATGCCTGGTG TAAGATGTGC 
AGCGAAGGCG GCCTTCCTTC TGAGATGCAG GACCTGGAGC TGGCAATCCA CCATCACCAG 
TCCTTATATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGATGG CAAAGCACTA 
CTGGATGTCC TGCAGCGTCC CCTGAGTCCT GGGAACTCTG AGTCCCTCAC AGCCACAGCC 
AACTACTCCA AGGCGGTCCA CCAGGTGCTG GATGTGGTCC ACGAGGTGCT GCACCATCAG 
CGACGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TTCACCAGCG GCTGCAGCTG 
TGTGTCTTCC AGCAGGATGT GCAGCAGGTG CTGGACTGGA TTGAAAACCA TGGCGAGGCC 
TTTCTCAGCA AACACACTGG GGTTGGCAAG TCCTTGCATC GAGCACGTGC ACTACAGAAG 
AGGCATGATG ACTTTGAAGA AGTGGCACAG AATACATACA CCAATGCGGA CAAGCTCCTA 
GAAGCAGCGG AGCAGCTGGC TCAGACTGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA 
GCCCGGCACC TGGAGGTGCG CATCCAAGAC TTTGTGCGCA GAGTGGAGCA GCGGAAGCTG 
CTTCTGGACA TGTCGGTCTC TTTCCACACG CACACCAAAG AGCTGTGGAC ATGGATGGAG 
GACCTTCAGA AGGAGGTGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAG 
CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACCCTGG ATGCCACTCT CAATGTCATC 
AAGGAAGGCG AAGACCTTAT CCAGCAGCTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC 
ACCGAGGCCA GGGACTCAGC GATGTCCAAC AACAAGACAC CCCACAGTAG CTCTATCAGC 
CATATTGAGT CTGTCCTCCA GCAGCTGGAC GATGCTCAGG TACAGATGGA AGAGCTGTTT 
CACGAGCGGA AGATCAAGCT GGACATCTTC CTACAGCTGC GCATCTTTGA ACAGTACACC 
ATTGAGGTCA CTGCAGAGCT GGATGCCTGG AACGAGGACT TGCTCCGGCA GATGAATGAT 
TTCAACACAG AGGATCTGAC CCTGGCTGAG CAGCGGCTAC AGCGCCACAC AGAACGGAAG 
CTTGCCATGA ACAACATGAC GTTCGAGGTC ATCCAGCAGG GCCAGGACCT GCACCAGTAC 
ATCATGGAAG TGCAAGCGTC AGGAATCGAG TTAATCTGTG AGAAGGACCT TGACCTGGCA 
GCTCAGGTGC AGGAGCTGCT GGAGTTTCTC CATGAGAAGC AGCACGAGCT GGAGCTCAAT 
GCGGAGCAGA CGCACAAGCG GCTAGAGCAG TGTCTCCAGC TGAGGCACCT CCAGGCTGAG 
GTCAAACAGG TTCTGGGATG GATCCGCAAT GGAGAGTCGA TGCTCAACGC CAGCCTGGTC 
AACGCCAGCT CTTTGTCGGA GGCAGAGCAG CTGCAGCGGG AGCATGAACA GTTCCAGCTA 
GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CGCACCAGAG CGCCCTGCAG 
GTACAGCAGA AGGCAGAGGC ACTGCTCCAG GCCGGCCACT ACGACGCAGA CGCCATCCGG 
GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAACAGCTCA TGCTAAAGAT GGAGGACCGG 
CTGAAGCTTG TCAACGCCTC TGTGGCCTTT TACAAGACCT CTGAGCAGGT GTGCAGTGTT 
CTGGAGAGCT TGGAGCAGGA GTACCGGAGA GACGAGGACT GGTGTGGTGG GAGAGACAAG 
CTGGGGCCGG CGGCAGAGAT GGACCACGTT ATTCCACTCC TCAGCAAGCA CCTGGAGCAG 
AAGGAGGCCT TCCTGAAGGC CTGCACGCTG GCAAGGCGGA ACGCCGAGGT GTTCCTCAAA 
TACATCCACA GGAACAATGT GAGCATGCCT AGTGTTGCCA GCCACACCCG GGGGCCTGAA 
CAGCAAGTAA AAGCCATCTT GAGCGAGCTC TTGCAGAGGG AGAATCGAGT CCTGCACTTC 
TGGACCTTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ATGTGGTGTT TGAGCGCAGT 
GCTAAGCAGG CACTGGACTG GATCCAAGAA ACAGGAGAAT ATTACCTCTC AACACATACC 
TCCACCGGAG AGACCACGGA GGAGACTCAG GAGCTGCTGA AAGAGTATGG AGAATTCAGG 
GTGCCTGCCA AGCAAACGAA GGAGAAGGTG AAACTTCTGA TCCAGCTAGC AGACAGCTTT 
GTGGAAAAGG GCCACATCCA CGCCACAGAG ATACGGAAAT GGGTGACCAC GGTGGACAAG 
CACTACCGCG ATTTCTCCCT GAGGATGGGG AAGTACCGGT ACTCGCTGGA GAAGGCGCTG 
GGGGTCAACA CGGAGGATAA CAAGGACCTA GAGCTGGACA TCATCCCAGC AAGCCTTTCG 
GATCGGGAGG TCAAGCTCCG GGACGCTAAC CATGAGATCA ATGAAGAGAA ACGGAAGTCA 
GCTCGAAAGA AAGAGTTCAT TATGGCTGAA CTGCTCCAAA CTGAGAAGGC TTATGTAAGG 
GACTTGCACG AGTGCTTGGA GACCTACCTG TGGGAGATGA CTAGTGGTGT GGAAGAGATA 
CCCCCCGGCA TCCTCAATAA GGAACATATC ATCTTCGGCA ACATCCAGGA GATCTATGAC 
TTCCATAACA ACATCTTCCT CAAAGAGCTG GAGAAGTACG AGCAGCTGCC TGAGGATGTG 
GGACACTGCT TTGTTACCTG GGCAGACAAA TTTCAGATGT ATGTCACCTA CTGTAAAAAC 
AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCGGGCA CCTTCTTTGA TGAGATCCAG 
CAGCGGCATG GCCTGGCCAA CTCCATCTCC TCCTACCTAA TTAAGCCTGT CCAGAGGGTT 
ACCAAGTACC AGCTGCTCCT GAAGGAACTT CTAACTTGCT GTGAAGAAGG GAAAGGAGAG 
CTGAAGGATG GCCTGGAGGT GATGCTCAGT GTCCCCAAGA AAGCCAACGA TGCCATGCAT 
GTCAGCATGC TGGAAGGGTT CGATGAAAAC TTGGATGTAC AGGGGGAGTT GATTCTCCAG 
GATGCCTTTC AAGTGTGGGA CCCAAAGTCG CTGATCCGGA AGGGGCGGGA GCGCCATCTG 
TTCCTCTTTG AGATCTCCTT GGTGTTTAGC AAAGAGATCA AAGACTCTTC AGGACACACG 
AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA GCATGTAGAA 
GGTGACCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA 
GTGCTCAAAG CTTCCAACAT CGAAACCAAG CAGGAATGGA TCAAGAACAT TCGAGAAGTG 
ATTCAGGAGA GGATCATTCA TCTTAAAGGC GCTTTAAAGG AGCCAATTCA GCTCCCCAAA 
ACACCAGCCA AACTGAGGAA CAATAGTAAG AGGGATGGGG TAGAGGATGG TGACAGCCAA 
GGGGATGGGA GCAGCCAGCC AGATACCATA TCCATTGCCT CCAGGACCTC TCAGAACACA 
GTGGAAAGTG ACAAGCTCTC AGGTGGATGT GAGCTGACCG TGGTCCTCCA GGACTTCAGC 
GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGCCAGA CGGTGGAGCT GCTGGAACGA 
CCGAGTGAGA GGCCTGGCTG GTGTCTGGTC CGCACCACAG AGCGGAGCCC CCCACAGGAA 
GGCCTGGTAC CCAGCAGCGC CCTGTGCATC TCACACTCTC GCAGCAGCGT GGAAATGGAC 
TGCTTCTTCC CCTTGAAAGA TTCATACTCT CATTCCTCCA GTGAGAATGG AGGCAAATCC 
GAGTCCGTGG CCCACCTGCA GTCTCAGCCC TCCCTGAACT CCATCCACAG TTCCCCTGGT 
CCCAAGCGCT CCACCAACAC CCTCAAAAAG TGGCTGACCA GCCCCGTGCG CCGCCTCAAC 
AGCGGAAAAG CCGACGGGAA CATCAAAAAG CAGAAGAAAG TCCGGGATGG GCGGAAGAGC 
TTCGATCTGG GATCGCCCAA GCCTGGGGAT GAGACGACAC CTCAGGGAGA CAGCGCTGAC 
GAGAAGAGCA AGAAGGGTTG GGGCGAAGAC GAGCCAGATG AAGAGTCACA CACACCCCTC 
CCTCCGCCCA TGAAGATCTT TGACAACGAC CCCACACAGG ATGAGATGTC GTCTTTGCTA 
GCAGCCCGGC AGGCTCCCCC TGACGTGCCT ACTGCTGCAG ACCTTGTCAG TGCAATAGAA 
AAGTTGGTCA AAAATAAGCT GACTCTGGAA GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC 
CCTACAGGCT GCCTGAATGA GGGGATGACC CCTCCCACAC CTCCTAGAAA CCTGGAAGAA 
GAACAGAAAG CCAAGGCGCT GAGAGGCAGG ATGTTTGTCC TGAATGAGCT GGTACAGACA 
GAGAAGGACT ACGTCAAGGA CCTGGGGATT GTGGTGGAGG GCTTCATGAA GAGAATAGAA 
GAGAAGGGGG TCCCTGAGGA CATGCGAGGG AAGGAAAAGA TCGTGTTTGG AAATATCCAC 
CAGATTTACG ACTGGCATAA AGATTTTTTC CTGGCTGAAC TGGAAAAATG TATCCAAGAG 
CAAGACAGAT TGGCCCAGCT CTTCATTAAA CACGAGCGGA AGCTGCACAT CTACGTGTGG 
TACTGCCAGA ACAAGCCGCG TTCCGAGTAC ATCGTGGCTG AGTACGATGC CTATTTTGAG 
GAGGTTAAAC AAGAGATCAA TCAAAGGCTG ACCCTTAGTG ACTTCCTGAT CAAGCCCATT 
CAGAGAATAA CAAAGTACCA GCTGCTACTC AAGGACTTCC TGCGATACAG CGAGAAGGCT 
GGTTTGGAGT GTTCCGATAT TGAGAAAGCA GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC 
TGTAATGACA TGATGAATCT GGGACGCCTG CAGGGCTTCG AGGGCACCCT GACCGCCCAG 
GGCAAGCTAC TGCAGCAGGA CACATTCTAT GTGATCGAGC TGGATGCCGG CATGCAGTCC 
CGGACCAAGG AGAGGCGTGT GTTTCTCTTT GAGCAGATTG TCATCTTCAG TGAACTGCTC 
CGAAAGGGGT CCCTCACCCC AGGCTACATG TTCAAAAGAA GCATCAAGAT GAATTACTTG 
GTCCTGGAAG ACAATGTGGA TGGTGACCCC TGCAAATTTG CACTCATGAA CAGGGAGACT 
TCAGAAAGGG TCATTCTACA GGCTGCCAAC TCAGACATCC AGCAGGCCTG GGTGCAGGAC 
ATCAACCAAG TCCTAGAAAC ACAGAGGGAC TTCTTAAATG CACTACAGTC ACCCATTGAG 
TATCAGCGGA AGGAGAGAAG CACAGCTGTG ATCCGGTCCC AGCCCCCGAG GGTTCCACAA 
GCCAGCCCCA GGCCCTACTC TTCTGGCCCG GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC 
AGCTATAACC CGCCTCTGCC ACCCCTGAAG ATATCTACCT CTAATGGCAG TCCAGGGTTC 
GACTACCATC AGCCTGGGGA CAAGTTTGAC GCTAGCAAGC AGAACGACCT GGGAGGCTGC 
AATGGGACAT CAACCATGAC TGTAATCAAA GATTACTATG CACTGAAGGA GAATGAAATC 
TGCGTGAGCC AAGGTGAGGT GGTACAGGTC CTCGCCGTCA ACCAGCAGAA CATGTGTCTG 
GTGTACCAGC CTGCCAGCGA CCATTCCCCG GCAGCAGAGG GCTGGGTCCC AGGCAGCATC 
CTGGCGCCCC TCGCCAAAGC CACAGCAGCG GCCGAAAGTA GTGACGGGAG CATCAAGAAG 
TCATGCTCTT GGCACACTCT ACGCATGAGA AAGCGGGCAG ACGTGGAGAA CTCGGGTAAA 
AATGAAGCCA CAGGGCCTCG TAAACCCAAG GATATTCTGG GCAACAAAGT CTCTGTTAAA 
GAGACGAACA GTTCCGAGGA GTCAGAGTGT GATGATCTTG ACCCTAATAC TAGTATGGAG 
ATCTTGAATC CAAATTTCAT CCAGGAAGTG GCTCCAGAAT TCCTCGTGCC CTTAGTAGAT 
GTGACCTGTT TGCTTGGAGA CACAGTGCTA CTGCAGTGTA AAGCCTGTGG GCGGCCAAAG 
CCCAGCATCA CCTGGAAGGG TCCGGATCAG AACATCCTAG ACACTGACAA CAGTTCAGCA 
ACATACACCA TCTCCTCCTG TGATTCTGGG GAAAGCACCC TCAAGATCTG CAACCTGATG 
CCCCAGGATA GTGGGATTTA TACCTGCATA GCAGCAAACG ACCACGGGAC TGCATCCACA 
TCTGCTACAG TTAAGGTGCA AGGTGTCCCC GCAGCCCCCA ACCGCCCAAT CGCACAGGAG 
AGGAGCTGCA CCTCCGTCAT CCTCCGCTGG CTGCCCCCAG CCAGCACCGG AAACTGCACT 
ATTTCGGGGT ACACAGTGGA GTATCGCGAG GAAGGTTCCC AGGTCTGGCA GCAATCCGTA 
GCTTCGACCT TGGACACTTA CCTGGTCATT GAAGACCTTA GTCCTGGGTG CCCTTATCAG 
TTCAGGGTCA GTGCCAGCAA CCCCTGGGGA ATCAGCCTTC CCAGTGAGCC CTCGGAGTTT 
GTGCGCCTTC CAGAGTACGA TGCAGCCGCC GACGGTGCCA CCATTTCTTG GAAGGAAAAT 
TTTGATTCTG CTTACACTGA ACTGAACGAG ATTGGCAGAG GCCGTTTCTC TATTGTAAAG 
AAATGCATTC ACAAAGCTAC CCGCAAAGAT GTTGCTGTGA AATTTGTGAG CAAAAAAATG 
AAGAAGAAGG AGCAGGCTGC TCACGAGGCC GCCCTGCTCC AGCACCTGCA GCACCCTCAG 
TATGTCACGC TCCATGACAC CTACGAGTCC CCCACATCCT ACATCCTGAT TTTGGAACTG 
ATGGATGACG GCCGACTCTT GGACTACCTT ATGAATCATG ATGAGCTGAT GGAAGAAAAG 
GTAGCTTTCT ACATTCGAGA CATCATGGAA GCTCTGCAGT ACCTGCACAA CTGTAGGGTC 
GCACATTTGG ACATAAAGCC CGAAAACCTG CTCATTGACC TCCGGATCCC AGTGCCTCGA 
GTGAAGCTCA TCGACTTGGA AGACGCTGTC CAGATCTCAG GCCACTTCCA TATCCACCAT 
CTGCTGGGGA ACCCTGAGTT CGCTGCCCCC GAAGTGATCC AAGGCATCCC TGTCTCCCTG 
GGCACAGACA TCTGGAGCAT CGGGGTTCTG ACATATGTCA TGCTTAGTGG GGTCTCCCCC 
TTCTTAGATG AGAGCAAAGA GGAAACCTGT ATCAACGTGT GCAGGGTGGA CTTTAGCTTC 
CCCCACGAGT ACTTCTGCGG TGTGAGCAAT GCTGCCAGGG ATTTCATCAA CGTAATCTTA 
CAGGAAGACT TCCGGAGGCG GCCCACAGCA GCCACGTGCC TGCAGCATCC CTGGCTACAG 
CCCCACAACG GCAGCTACTC CAAGATCCCC TTGGACACCT CCCGTCTGGC CTGCTTCATA 
GAACGCCGCA AGCACCAGAA TGATGTGCGG CCCATTCCCA ATGTCAAGAG TTACATCGTC 
AATAGGGTGA ACCAAGGGAC CTCGCTATCC CACAACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244257.1
CDS	99..9080
Translation	MNPPEGASEEGGAADSDVDAFFRTGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245955.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245955.2

ORF Insert Sequence:

ATGAACCCCC CGGAGGGGGC ATCGGAGGAA GGGGGAGCCG CCGACTCGGA CGTGGACGCC 
TTTTTCCGGA CAGGATCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCTATC 
CTAAAGGAGA AAGTGGCCTT TGTGTCTGGG GGGCGTGACA AGCGAGGTGG ACCCATCCTG 
ACCTTCCCTG CCCGAAGCAA TCATGACAGA ATAAGACAGG AAGACCTACG GAAACTTGTG 
ACGTATTTGG CCAGCGTGCC AAGTGAGGAC GTTTGCAAAC GTGGCTTCAC CGTCATCATC 
GACATGCGGG GCTCCAAGTG GGATCTCATC AAGCCCCTCC TCAAGACGCT GCAGGAAGCC 
TTCCCCGCCG AGATCCATGT GGCCCTCATC ATCAAGCCTG ACAACTTCTG GCAGAAGCAG 
AAGACCAACT TTGGCAGCTC CAAATTCATC TTTGAGACAA GCATGGTGTC TGTGGAGGGC 
CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACAGAGGAGT TTGACGGCTC CCTGGATTAC 
AACCATGAGG AGTGGATTGA GTTGCGCCTC TCCCTGGAGG AGTTCTTCAA CAGTGCGGTC 
CACCTGCTCT CACGCCTTGA GGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCTGTG 
GATGTGGAGG GCTCTCGGAG GCTTATCGAC GAGCACACGC AACTTAAGAA AAAGGTGCTG 
AAGGCCCCCG TGGAAGAGCT GGACCGGGAG GGGCAGCGTC TACTGCAGTG CATCCGCTGC 
AGCGATGGCT TCTCAGGGCG CAACTGCATC CCAGGAAGTG CCGACTTCCA GAGCCTTGTA 
CCCAAGATCA CCAGCCTCCT GGACAAGCTG CATTCCACCC GACAACACCT GCACCAAATG 
TGGCACGTGC GAAAGCTCAA GCTGGATCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT 
GCTGAGAAGA TGTTCGACTG GATAAGCCAC AACAAGGAGT TATTTCTTCA GAGCCACACA 
GAGATCGGAG TCAGCTACCA GCATGCACTA GACCTCCAGA CACAGCACAA TCACTTTGCC 
ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTCGC TTCCCGCCTC 
TCAGAGGCCG GCCATTATGC CTCCCAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG 
GAGTGGAAGA GCTTTGCCGC TGCCCTGGAT GAACGCAGTA CAATCCTGGC CATGTCTGCT 
GTGTTCCACC AGAAGGCAGA GCAGTTCCTG TCAGGCGTGG ATGCCTGGTG TAAGATGTGC 
AGCGAAGGCG GCCTTCCTTC TGAGATGCAG GACCTGGAGC TGGCAATCCA CCATCACCAG 
TCCTTATATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGATGG CAAAGCACTA 
CTGGATGTCC TGCAGCGTCC CCTGAGTCCT GGGAACTCTG AGTCCCTCAC AGCCACAGCC 
AACTACTCCA AGGCGGTCCA CCAGGTGCTG GATGTGGTCC ACGAGGTGCT GCACCATCAG 
CGACGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TTCACCAGCG GCTGCAGCTG 
TGTGTCTTCC AGCAGGATGT GCAGCAGGTG CTGGACTGGA TTGAAAACCA TGGCGAGGCC 
TTTCTCAGCA AACACACTGG GGTTGGCAAG TCCTTGCATC GAGCACGTGC ACTACAGAAG 
AGGCATGATG ACTTTGAAGA AGTGGCACAG AATACATACA CCAATGCGGA CAAGCTCCTA 
GAAGCAGCGG AGCAGCTGGC TCAGACTGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA 
GCCCGGCACC TGGAGGTGCG CATCCAAGAC TTTGTGCGCA GAGTGGAGCA GCGGAAGCTG 
CTTCTGGACA TGTCGGTCTC TTTCCACACG CACACCAAAG AGCTGTGGAC ATGGATGGAG 
GACCTTCAGA AGGAGGTGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAG 
CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACCCTGG ATGCCACTCT CAATGTCATC 
AAGGAAGGCG AAGACCTTAT CCAGCAGCTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC 
ACCGAGGCCA GGGACTCAGC GATGTCCAAC AACAAGACAC CCCACAGTAG CTCTATCAGC 
CATATTGAGT CTGTCCTCCA GCAGCTGGAC GATGCTCAGG TACAGATGGA AGAGCTGTTT 
CACGAGCGGA AGATCAAGCT GGACATCTTC CTACAGCTGC GCATCTTTGA ACAGTACACC 
ATTGAGGTCA CTGCAGAGCT GGATGCCTGG AACGAGGACT TGCTCCGGCA GATGAATGAT 
TTCAACACAG AGGATCTGAC CCTGGCTGAG CAGCGGCTAC AGCGCCACAC AGAACGGAAG 
CTTGCCATGA ACAACATGAC GTTCGAGGTC ATCCAGCAGG GCCAGGACCT GCACCAGTAC 
ATCATGGAAG TGCAAGCGTC AGGAATCGAG TTAATCTGTG AGAAGGACCT TGACCTGGCA 
GCTCAGGTGC AGGAGCTGCT GGAGTTTCTC CATGAGAAGC AGCACGAGCT GGAGCTCAAT 
GCGGAGCAGA CGCACAAGCG GCTAGAGCAG TGTCTCCAGC TGAGGCACCT CCAGGCTGAG 
GTCAAACAGG TTCTGGGATG GATCCGCAAT GGAGAGTCGA TGCTCAACGC CAGCCTGGTC 
AACGCCAGCT CTTTGTCGGA GGCAGAGCAG CTGCAGCGGG AGCATGAACA GTTCCAGCTA 
GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CGCACCAGAG CGCCCTGCAG 
GTACAGCAGA AGGCAGAGGC ACTGCTCCAG GCCGGCCACT ACGACGCAGA CGCCATCCGG 
GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAACAGCTCA TGCTAAAGAT GGAGGACCGG 
CTGAAGCTTG TCAACGCCTC TGTGGCCTTT TACAAGACCT CTGAGCAGGT GTGCAGTGTT 
CTGGAGAGCT TGGAGCAGGA GTACCGGAGA GACGAGGACT GGTGTGGTGG GAGAGACAAG 
CTGGGGCCGG CGGCAGAGAT GGACCACGTT ATTCCACTCC TCAGCAAGCA CCTGGAGCAG 
AAGGAGGCCT TCCTGAAGGC CTGCACGCTG GCAAGGCGGA ACGCCGAGGT GTTCCTCAAA 
TACATCCACA GGAACAATGT GAGCATGCCT AGTGTTGCCA GCCACACCCG GGGGCCTGAA 
CAGCAAGTAA AAGCCATCTT GAGCGAGCTC TTGCAGAGGG AGAATCGAGT CCTGCACTTC 
TGGACCTTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ATGTGGTGTT TGAGCGCAGT 
GCTAAGCAGG CACTGGACTG GATCCAAGAA ACAGGAGAAT ATTACCTCTC AACACATACC 
TCCACCGGAG AGACCACGGA GGAGACTCAG GAGCTGCTGA AAGAGTATGG AGAATTCAGG 
GTGCCTGCCA AGCAAACGAA GGAGAAGGTG AAACTTCTGA TCCAGCTAGC AGACAGCTTT 
GTGGAAAAGG GCCACATCCA CGCCACAGAG ATACGGAAAT GGGTGACCAC GGTGGACAAG 
CACTACCGCG ATTTCTCCCT GAGGATGGGG AAGTACCGGT ACTCGCTGGA GAAGGCGCTG 
GGGGTCAACA CGGAGGATAA CAAGGACCTA GAGCTGGACA TCATCCCAGC AAGCCTTTCG 
GATCGGGAGG TCAAGCTCCG GGACGCTAAC CATGAGATCA ATGAAGAGAA ACGGAAGTCA 
GCTCGAAAGA AAGAGTTCAT TATGGCTGAA CTGCTCCAAA CTGAGAAGGC TTATGTAAGG 
GACTTGCACG AGTGCTTGGA GACCTACCTG TGGGAGATGA CTAGTGGTGT GGAAGAGATA 
CCCCCCGGCA TCCTCAATAA GGAACATATC ATCTTCGGCA ACATCCAGGA GATCTATGAC 
TTCCATAACA ACATCTTCCT CAAAGAGCTG GAGAAGTACG AGCAGCTGCC TGAGGATGTG 
GGACACTGCT TTGTTACCTG GGCAGACAAA TTTCAGATGT ATGTCACCTA CTGTAAAAAC 
AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCGGGCA CCTTCTTTGA TGAGATCCAG 
CAGCGGCATG GCCTGGCCAA CTCCATCTCC TCCTACCTAA TTAAGCCTGT CCAGAGGGTT 
ACCAAGTACC AGCTGCTCCT GAAGGAACTT CTAACTTGCT GTGAAGAAGG GAAAGGAGAG 
CTGAAGGATG GCCTGGAGGT GATGCTCAGT GTCCCCAAGA AAGCCAACGA TGCCATGCAT 
GTCAGCATGC TGGAAGGGTT CGATGAAAAC TTGGATGTAC AGGGGGAGTT GATTCTCCAG 
GATGCCTTTC AAGTGTGGGA CCCAAAGTCG CTGATCCGGA AGGGGCGGGA GCGCCATCTG 
TTCCTCTTTG AGATCTCCTT GGTGTTTAGC AAAGAGATCA AAGACTCTTC AGGACACACG 
AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA GCATGTAGAA 
GGTGACCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA 
GTGCTCAAAG CTTCCAACAT CGAAACCAAG CAGGAATGGA TCAAGAACAT TCGAGAAGTG 
ATTCAGGAGA GGATCATTCA TCTTAAAGGC GCTTTAAAGG AGCCAATTCA GCTCCCCAAA 
ACACCAGCCA AACTGAGGAA CAATAGTAAG AGGGATGGGG TAGAGGATGG TGACAGCCAA 
GGGGATGGGA GCAGCCAGCC AGATACCATA TCCATTGCCT CCAGGACCTC TCAGAACACA 
GTGGAAAGTG ACAAGCTCTC AGGTGGATGT GAGCTGACCG TGGTCCTCCA GGACTTCAGC 
GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGCCAGA CGGTGGAGCT GCTGGAACGA 
CCGAGTGAGA GGCCTGGCTG GTGTCTGGTC CGCACCACAG AGCGGAGCCC CCCACAGGAA 
GGCCTGGTAC CCAGCAGCGC CCTGTGCATC TCACACTCTC GCAGCAGCGT GGAAATGGAC 
TGCTTCTTCC CCTTGAAAGA TTCATACTCT CATTCCTCCA GTGAGAATGG AGGCAAATCC 
GAGTCCGTGG CCCACCTGCA GTCTCAGCCC TCCCTGAACT CCATCCACAG TTCCCCTGGT 
CCCAAGCGCT CCACCAACAC CCTCAAAAAG TGGCTGACCA GCCCCGTGCG CCGCCTCAAC 
AGCGGAAAAG CCGACGGGAA CATCAAAAAG CAGAAGAAAG TCCGGGATGG GCGGAAGAGC 
TTCGATCTGG GATCGCCCAA GCCTGGGGAT GAGACGACAC CTCAGGGAGA CAGCGCTGAC 
GAGAAGAGCA AGAAGGGTTG GGGCGAAGAC GAGCCAGATG AAGAGTCACA CACACCCCTC 
CCTCCGCCCA TGAAGATCTT TGACAACGAC CCCACACAGG ATGAGATGTC GTCTTTGCTA 
GCAGCCCGGC AGGCTCCCCC TGACGTGCCT ACTGCTGCAG ACCTTGTCAG TGCAATAGAA 
AAGTTGGTCA AAAATAAGCT GACTCTGGAA GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC 
CCTACAGGCT GCCTGAATGA GGGGATGACC CCTCCCACAC CTCCTAGAAA CCTGGAAGAA 
GAACAGAAAG CCAAGGCGCT GAGAGGCAGG ATGTTTGTCC TGAATGAGCT GGTACAGACA 
GAGAAGGACT ACGTCAAGGA CCTGGGGATT GTGGTGGAGG GCTTCATGAA GAGAATAGAA 
GAGAAGGGGG TCCCTGAGGA CATGCGAGGG AAGGAAAAGA TCGTGTTTGG AAATATCCAC 
CAGATTTACG ACTGGCATAA AGATTTTTTC CTGGCTGAAC TGGAAAAATG TATCCAAGAG 
CAAGACAGAT TGGCCCAGCT CTTCATTAAA CACGAGCGGA AGCTGCACAT CTACGTGTGG 
TACTGCCAGA ACAAGCCGCG TTCCGAGTAC ATCGTGGCTG AGTACGATGC CTATTTTGAG 
GAGGTTAAAC AAGAGATCAA TCAAAGGCTG ACCCTTAGTG ACTTCCTGAT CAAGCCCATT 
CAGAGAATAA CAAAGTACCA GCTGCTACTC AAGGACTTCC TGCGATACAG CGAGAAGGCT 
GGTTTGGAGT GTTCCGATAT TGAGAAAGCA GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC 
TGTAATGACA TGATGAATCT GGGACGCCTG CAGGGCTTCG AGGGCACCCT GACCGCCCAG 
GGCAAGCTAC TGCAGCAGGA CACATTCTAT GTGATCGAGC TGGATGCCGG CATGCAGTCC 
CGGACCAAGG AGAGGCGTGT GTTTCTCTTT GAGCAGATTG TCATCTTCAG TGAACTGCTC 
CGAAAGGGGT CCCTCACCCC AGGCTACATG TTCAAAAGAA GCATCAAGAT GAATTACTTG 
GTCCTGGAAG ACAATGTGGA TGGTGACCCC TGCAAATTTG CACTCATGAA CAGGGAGACT 
TCAGAAAGGG TCATTCTACA GGCTGCCAAC TCAGACATCC AGCAGGCCTG GGTGCAGGAC 
ATCAACCAAG TCCTAGAAAC ACAGAGGGAC TTCTTAAATG CACTACAGTC ACCCATTGAG 
TATCAGCGGA AGGAGAGAAG CACAGCTGTG ATCCGGTCCC AGCCCCCGAG GGTTCCACAA 
GCCAGCCCCA GGCCCTACTC TTCTGGCCCG GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC 
AGCTATAACC CGCCTCTGCC ACCCCTGAAG ATATCTACCT CTAATGGCAG TCCAGGGTTC 
GACTACCATC AGCCTGGGGA CAAGTTTGAC GCTAGCAAGC AGAACGACCT GGGAGGCTGC 
AATGGGACAT CAACCATGAC TGTAATCAAA GATTACTATG CACTGAAGGA GAATGAAATC 
TGCGTGAGCC AAGGTGAGGT GGTACAGGTC CTCGCCGTCA ACCAGCAGAA CATGTGTCTG 
GTGTACCAGC CTGCCAGCGA CCATTCCCCG GCAGCAGAGG GCTGGGTCCC AGGCAGCATC 
CTGGCGCCCC TCGCCAAAGC CACAGCAGCG GCCGAAAGTA GTGACGGGAG CATCAAGAAG 
TCATGCTCTT GGCACACTCT ACGCATGAGA AAGCGGGCAG ACGTGGAGAA CTCGGGTAAA 
AATGAAGCCA CAGGGCCTCG TAAACCCAAG GATATTCTGG GCAACAAAGT CTCTGTTAAA 
GAGACGAACA GTTCCGAGGA GTCAGAGTGT GATGATCTTG ACCCTAATAC TAGTATGGAG 
ATCTTGAATC CAAATTTCAT CCAGGAAGTG GCTCCAGAAT TCCTCGTGCC CTTAGTAGAT 
GTGACCTGTT TGCTTGGAGA CACAGTGCTA CTGCAGTGTA AAGCCTGTGG GCGGCCAAAG 
CCCAGCATCA CCTGGAAGGG TCCGGATCAG AACATCCTAG ACACTGACAA CAGTTCAGCA 
ACATACACCA TCTCCTCCTG TGATTCTGGG GAAAGCACCC TCAAGATCTG CAACCTGATG 
CCCCAGGATA GTGGGATTTA TACCTGCATA GCAGCAAACG ACCACGGGAC TGCATCCACA 
TCTGCTACAG TTAAGGTGCA AGGTGTCCCC GCAGCCCCCA ACCGCCCAAT CGCACAGGAG 
AGGAGCTGCA CCTCCGTCAT CCTCCGCTGG CTGCCCCCAG CCAGCACCGG AAACTGCACT 
ATTTCGGGGT ACACAGTGGA GTATCGCGAG GAAGGTTCCC AGGTCTGGCA GCAATCCGTA 
GCTTCGACCT TGGACACTTA CCTGGTCATT GAAGACCTTA GTCCTGGGTG CCCTTATCAG 
TTCAGGGTCA GTGCCAGCAA CCCCTGGGGA ATCAGCCTTC CCAGTGAGCC CTCGGAGTTT 
GTGCGCCTTC CAGAGTACGA TGCAGCCGCC GACGGTGCCA CCATTTCTTG GAAGGAAAAT 
TTTGATTCTG CTTACACTGA ACTGAACGAG ATTGGCAGAG GCCGTTTCTC TATTGTAAAG 
AAATGCATTC ACAAAGCTAC CCGCAAAGAT GTTGCTGTGA AATTTGTGAG CAAAAAAATG 
AAGAAGAAGG AGCAGGCTGC TCACGAGGCC GCCCTGCTCC AGCACCTGCA GCACCCTCAG 
TATGTCACGC TCCATGACAC CTACGAGTCC CCCACATCCT ACATCCTGAT TTTGGAACTG 
ATGGATGACG GCCGACTCTT GGACTACCTT ATGAATCATG ATGAGCTGAT GGAAGAAAAG 
GTAGCTTTCT ACATTCGAGA CATCATGGAA GCTCTGCAGT ACCTGCACAA CTGTAGGGTC 
GCACATTTGG ACATAAAGCC CGAAAACCTG CTCATTGACC TCCGGATCCC AGTGCCTCGA 
GTGAAGCTCA TCGACTTGGA AGACGCTGTC CAGATCTCAG GCCACTTCCA TATCCACCAT 
CTGCTGGGGA ACCCTGAGTT CGCTGCCCCC GAAGTGATCC AAGGCATCCC TGTCTCCCTG 
GGCACAGACA TCTGGAGCAT CGGGGTTCTG ACATATGTCA TGCTTAGTGG GGTCTCCCCC 
TTCTTAGATG AGAGCAAAGA GGAAACCTGT ATCAACGTGT GCAGGGTGGA CTTTAGCTTC 
CCCCACGAGT ACTTCTGCGG TGTGAGCAAT GCTGCCAGGG ATTTCATCAA CGTAATCTTA 
CAGGAAGACT TCCGGAGGCG GCCCACAGCA GCCACGTGCC TGCAGCATCC CTGGCTACAG 
CCCCACAACG GCAGCTACTC CAAGATCCCC TTGGACACCT CCCGTCTGGC CTGCTTCATA 
GAACGCCGCA AGCACCAGAA TGATGTGCGG CCCATTCCCA ATGTCAAGAG TTACATCGTC 
AATAGGGTGA ACCAAGGGAC CTCGCTATCC CACAACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245956.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244258.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X8, mRNA.

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CloneID	OMu75312
Clone ID Related Accession (Same CDS sequence)	XM_011245956.2 , XM_011245956.3
Accession Version	XM_011245956.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGTC CCTCTTTCAT 
GCCACTTCCT TGCAGAAGAC GCACCAGAGC GCCCTGCAGG TACAGCAGAA GGCAGAGGCA 
CTGCTCCAGG CCGGCCACTA CGACGCAGAC GCCATCCGGG AATGTGCTGA GAAGGTGGCC 
CTCCACTGGC AACAGCTCAT GCTAAAGATG GAGGACCGGC TGAAGCTTGT CAACGCCTCT 
GTGGCCTTTT ACAAGACCTC TGAGCAGGTG TGCAGTGTTC TGGAGAGCTT GGAGCAGGAG 
TACCGGAGAG ACGAGGACTG GTGTGGTGGG AGAGACAAGC TGGGGCCGGC GGCAGAGATG 
GACCACGTTA TTCCACTCCT CAGCAAGCAC CTGGAGCAGA AGGAGGCCTT CCTGAAGGCC 
TGCACGCTGG CAAGGCGGAA CGCCGAGGTG TTCCTCAAAT ACATCCACAG GAACAATGTG 
AGCATGCCTA GTGTTGCCAG CCACACCCGG GGGCCTGAAC AGCAAGTAAA AGCCATCTTG 
AGCGAGCTCT TGCAGAGGGA GAATCGAGTC CTGCACTTCT GGACCTTGAA GAAGCGGCGG 
TTAGACCAGT GTCAGCAATA TGTGGTGTTT GAGCGCAGTG CTAAGCAGGC ACTGGACTGG 
ATCCAAGAAA CAGGAGAATA TTACCTCTCA ACACATACCT CCACCGGAGA GACCACGGAG 
GAGACTCAGG AGCTGCTGAA AGAGTATGGA GAATTCAGGG TGCCTGCCAA GCAAACGAAG 
GAGAAGGTGA AACTTCTGAT CCAGCTAGCA GACAGCTTTG TGGAAAAGGG CCACATCCAC 
GCCACAGAGA TACGGAAATG GGTGACCACG GTGGACAAGC ACTACCGCGA TTTCTCCCTG 
AGGATGGGGA AGTACCGGTA CTCGCTGGAG AAGGCGCTGG GGGTCAACAC GGAGGATAAC 
AAGGACCTAG AGCTGGACAT CATCCCAGCA AGCCTTTCGG ATCGGGAGGT CAAGCTCCGG 
GACGCTAACC ATGAGATCAA TGAAGAGAAA CGGAAGTCAG CTCGAAAGAA AGAGTTCATT 
ATGGCTGAAC TGCTCCAAAC TGAGAAGGCT TATGTAAGGG ACTTGCACGA GTGCTTGGAG 
ACCTACCTGT GGGAGATGAC TAGTGGTGTG GAAGAGATAC CCCCCGGCAT CCTCAATAAG 
GAACATATCA TCTTCGGCAA CATCCAGGAG ATCTATGACT TCCATAACAA CATCTTCCTC 
AAAGAGCTGG AGAAGTACGA GCAGCTGCCT GAGGATGTGG GACACTGCTT TGTTACCTGG 
GCAGACAAAT TTCAGATGTA TGTCACCTAC TGTAAAAACA AGCCTGATTC CAACCAGCTG 
ATCCTGGAGC ATGCGGGCAC CTTCTTTGAT GAGATCCAGC AGCGGCATGG CCTGGCCAAC 
TCCATCTCCT CCTACCTAAT TAAGCCTGTC CAGAGGGTTA CCAAGTACCA GCTGCTCCTG 
AAGGAACTTC TAACTTGCTG TGAAGAAGGG AAAGGAGAGC TGAAGGATGG CCTGGAGGTG 
ATGCTCAGTG TCCCCAAGAA AGCCAACGAT GCCATGCATG TCAGCATGCT GGAAGGGTTC 
GATGAAAACT TGGATGTACA GGGGGAGTTG ATTCTCCAGG ATGCCTTTCA AGTGTGGGAC 
CCAAAGTCGC TGATCCGGAA GGGGCGGGAG CGCCATCTGT TCCTCTTTGA GATCTCCTTG 
GTGTTTAGCA AAGAGATCAA AGACTCTTCA GGACACACGA AATATGTTTA CAAGAACAAG 
CTACTGACCT CAGAGCTGGG TGTGACCGAG CATGTAGAAG GTGACCCCTG CAAATTCGCC 
TTGTGGTCTG GGCGCACCCC ATCCTCAGAC AATAAAACAG TGCTCAAAGC TTCCAACATC 
GAAACCAAGC AGGAATGGAT CAAGAACATT CGAGAAGTGA TTCAGGAGAG GATCATTCAT 
CTTAAAGGCG CTTTAAAGGA GCCAATTCAG CTCCCCAAAA CACCAGCCAA ACTGAGGAAC 
AATAGTAAGA GGGATGGGGT AGAGGATGGT GACAGCCAAG GGGATGGGAG CAGCCAGCCA 
GATACCATAT CCATTGCCTC CAGGACCTCT CAGAACACAG TGGAAAGTGA CAAGCTCTCA 
GGTGGATGTG AGCTGACCGT GGTCCTCCAG GACTTCAGCG CAGGCCACAG CAGCGAGCTG 
AGCATCCAGG TGGGCCAGAC GGTGGAGCTG CTGGAACGAC CGAGTGAGAG GCCTGGCTGG 
TGTCTGGTCC GCACCACAGA GCGGAGCCCC CCACAGGAAG GCCTGGTACC CAGCAGCGCC 
CTGTGCATCT CACACTCTCG CAGCAGCGTG GAAATGGACT GCTTCTTCCC CTTGAAAGAT 
TCATACTCTC ATTCCTCCAG TGAGAATGGA GGCAAATCCG AGTCCGTGGC CCACCTGCAG 
TCTCAGCCCT CCCTGAACTC CATCCACAGT TCCCCTGGTC CCAAGCGCTC CACCAACACC 
CTCAAAAAGT GGCTGACCAG CCCCGTGCGC CGCCTCAACA GCGGAAAAGC CGACGGGAAC 
ATCAAAAAGC AGAAGAAAGT CCGGGATGGG CGGAAGAGCT TCGATCTGGG ATCGCCCAAG 
CCTGGGGATG AGACGACACC TCAGGGAGAC AGCGCTGACG AGAAGAGCAA GAAGGGTTGG 
GGCGAAGACG AGCCAGATGA AGAGTCACAC ACACCCCTCC CTCCGCCCAT GAAGATCTTT 
GACAACGACC CCACACAGGA TGAGATGTCG TCTTTGCTAG CAGCCCGGCA GGCTCCCCCT 
GACGTGCCTA CTGCTGCAGA CCTTGTCAGT GCAATAGAAA AGTTGGTCAA AAATAAGCTG 
ACTCTGGAAG GAGGCTCTTA CCGGGGGAGC TTGAAGGACC CTACAGGCTG CCTGAATGAG 
GGGATGACCC CTCCCACACC TCCTAGAAAC CTGGAAGAAG AACAGAAAGC CAAGGCGCTG 
AGAGGCAGGA TGTTTGTCCT GAATGAGCTG GTACAGACAG AGAAGGACTA CGTCAAGGAC 
CTGGGGATTG TGGTGGAGGG CTTCATGAAG AGAATAGAAG AGAAGGGGGT CCCTGAGGAC 
ATGCGAGGGA AGGAAAAGAT CGTGTTTGGA AATATCCACC AGATTTACGA CTGGCATAAA 
GATTTTTTCC TGGCTGAACT GGAAAAATGT ATCCAAGAGC AAGACAGATT GGCCCAGCTC 
TTCATTAAAC ACGAGCGGAA GCTGCACATC TACGTGTGGT ACTGCCAGAA CAAGCCGCGT 
TCCGAGTACA TCGTGGCTGA GTACGATGCC TATTTTGAGG AGGTTAAACA AGAGATCAAT 
CAAAGGCTGA CCCTTAGTGA CTTCCTGATC AAGCCCATTC AGAGAATAAC AAAGTACCAG 
CTGCTACTCA AGGACTTCCT GCGATACAGC GAGAAGGCTG GTTTGGAGTG TTCCGATATT 
GAGAAAGCAG TGGAGTTAAT GTGCCTTGTC CCCAAGCGCT GTAATGACAT GATGAATCTG 
GGACGCCTGC AGGGCTTCGA GGGCACCCTG ACCGCCCAGG GCAAGCTACT GCAGCAGGAC 
ACATTCTATG TGATCGAGCT GGATGCCGGC ATGCAGTCCC GGACCAAGGA GAGGCGTGTG 
TTTCTCTTTG AGCAGATTGT CATCTTCAGT GAACTGCTCC GAAAGGGGTC CCTCACCCCA 
GGCTACATGT TCAAAAGAAG CATCAAGATG AATTACTTGG TCCTGGAAGA CAATGTGGAT 
GGTGACCCCT GCAAATTTGC ACTCATGAAC AGGGAGACTT CAGAAAGGGT CATTCTACAG 
GCTGCCAACT CAGACATCCA GCAGGCCTGG GTGCAGGACA TCAACCAAGT CCTAGAAACA 
CAGAGGGACT TCTTAAATGC ACTACAGTCA CCCATTGAGT ATCAGCGGAA GGAGAGAAGC 
ACAGCTGTGA TCCGGTCCCA GCCCCCGAGG GTTCCACAAG CCAGCCCCAG GCCCTACTCT 
TCTGGCCCGG TGGGCTCAGA GAAGCCCCCC AAGGGCTCCA GCTATAACCC GCCTCTGCCA 
CCCCTGAAGA TATCTACCTC TAATGGCAGT CCAGGGTTCG ACTACCATCA GCCTGGGGAC 
AAGTTTGACG CTAGCAAGCA GAACGACCTG GGAGGCTGCA ATGGGACATC AACCATGACT 
GTAATCAAAG ATTACTATGC ACTGAAGGAG AATGAAATCT GCGTGAGCCA AGGTGAGGTG 
GTACAGGTCC TCGCCGTCAA CCAGCAGAAC ATGTGTCTGG TGTACCAGCC TGCCAGCGAC 
CATTCCCCGG CAGCAGAGGG CTGGGTCCCA GGCAGCATCC TGGCGCCCCT CGCCAAAGCC 
ACAGCAGCGG CCGAAAGTAG TGACGGGAGC ATCAAGAAGT CATGCTCTTG GCACACTCTA 
CGCATGAGAA AGCGGGCAGA CGTGGAGAAC TCGGGTAAAA ATGAAGCCAC AGGGCCTCGT 
AAACCCAAGG ATATTCTGGG CAACAAAGTC TCTGTTAAAG AGACGAACAG TTCCGAGGAG 
TCAGAGTGTG ATGATCTTGA CCCTAATACT AGTATGGAGA TCTTGAATCC AAATTTCATC 
CAGGAAGTGG CTCCAGAATT CCTCGTGCCC TTAGTAGATG TGACCTGTTT GCTTGGAGAC 
ACAGTGCTAC TGCAGTGTAA AGCCTGTGGG CGGCCAAAGC CCAGCATCAC CTGGAAGGGT 
CCGGATCAGA ACATCCTAGA CACTGACAAC AGTTCAGCAA CATACACCAT CTCCTCCTGT 
GATTCTGGGG AAAGCACCCT CAAGATCTGC AACCTGATGC CCCAGGATAG TGGGATTTAT 
ACCTGCATAG CAGCAAACGA CCACGGGACT GCATCCACAT CTGCTACAGT TAAGGTGCAA 
GGTGTCCCCG CAGCCCCCAA CCGCCCAATC GCACAGGAGA GGAGCTGCAC CTCCGTCATC 
CTCCGCTGGC TGCCCCCAGC CAGCACCGGA AACTGCACTA TTTCGGGGTA CACAGTGGAG 
TATCGCGAGG AAGGTTCCCA GGTCTGGCAG CAATCCGTAG CTTCGACCTT GGACACTTAC 
CTGGTCATTG AAGACCTTAG TCCTGGGTGC CCTTATCAGT TCAGGGTCAG TGCCAGCAAC 
CCCTGGGGAA TCAGCCTTCC CAGTGAGCCC TCGGAGTTTG TGCGCCTTCC AGAGTACGAT 
GCAGCCGCCG ACGGTGCCAC CATTTCTTGG AAGGAAAATT TTGATTCTGC TTACACTGAA 
CTGAACGAGA TTGGCAGAGG CCGTTTCTCT ATTGTAAAGA AATGCATTCA CAAAGCTACC 
CGCAAAGATG TTGCTGTGAA ATTTGTGAGC AAAAAAATGA AGAAGAAGGA GCAGGCTGCT 
CACGAGGCCG CCCTGCTCCA GCACCTGCAG CACCCTCAGT ATGTCACGCT CCATGACACC 
TACGAGTCCC CCACATCCTA CATCCTGATT TTGGAACTGA TGGATGACGG CCGACTCTTG 
GACTACCTTA TGAATCATGA TGAGCTGATG GAAGAAAAGG TAGCTTTCTA CATTCGAGAC 
ATCATGGAAG CTCTGCAGTA CCTGCACAAC TGTAGGGTCG CACATTTGGA CATAAAGCCC 
GAAAACCTGC TCATTGACCT CCGGATCCCA GTGCCTCGAG TGAAGCTCAT CGACTTGGAA 
GACGCTGTCC AGATCTCAGG CCACTTCCAT ATCCACCATC TGCTGGGGAA CCCTGAGTTC 
GCTGCCCCCG AAGTGATCCA AGGCATCCCT GTCTCCCTGG GCACAGACAT CTGGAGCATC 
GGGGTTCTGA CATATGTCAT GCTTAGTGGG GTCTCCCCCT TCTTAGATGA GAGCAAAGAG 
GAAACCTGTA TCAACGTGTG CAGGGTGGAC TTTAGCTTCC CCCACGAGTA CTTCTGCGGT 
GTGAGCAATG CTGCCAGGGA TTTCATCAAC GTAATCTTAC AGGAAGACTT CCGGAGGCGG 
CCCACAGCAG CCACGTGCCT GCAGCATCCC TGGCTACAGC CCCACAACGG CAGCTACTCC 
AAGATCCCCT TGGACACCTC CCGTCTGGCC TGCTTCATAG AACGCCGCAA GCACCAGAAT 
GATGTGCGGC CCATTCCCAA TGTCAAGAGT TACATCGTCA ATAGGGTGAA CCAAGGGACC 
TCGCTATCCC ACAACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244258.1
CDS	4891..13851
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245956.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245956.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGTC CCTCTTTCAT 
GCCACTTCCT TGCAGAAGAC GCACCAGAGC GCCCTGCAGG TACAGCAGAA GGCAGAGGCA 
CTGCTCCAGG CCGGCCACTA CGACGCAGAC GCCATCCGGG AATGTGCTGA GAAGGTGGCC 
CTCCACTGGC AACAGCTCAT GCTAAAGATG GAGGACCGGC TGAAGCTTGT CAACGCCTCT 
GTGGCCTTTT ACAAGACCTC TGAGCAGGTG TGCAGTGTTC TGGAGAGCTT GGAGCAGGAG 
TACCGGAGAG ACGAGGACTG GTGTGGTGGG AGAGACAAGC TGGGGCCGGC GGCAGAGATG 
GACCACGTTA TTCCACTCCT CAGCAAGCAC CTGGAGCAGA AGGAGGCCTT CCTGAAGGCC 
TGCACGCTGG CAAGGCGGAA CGCCGAGGTG TTCCTCAAAT ACATCCACAG GAACAATGTG 
AGCATGCCTA GTGTTGCCAG CCACACCCGG GGGCCTGAAC AGCAAGTAAA AGCCATCTTG 
AGCGAGCTCT TGCAGAGGGA GAATCGAGTC CTGCACTTCT GGACCTTGAA GAAGCGGCGG 
TTAGACCAGT GTCAGCAATA TGTGGTGTTT GAGCGCAGTG CTAAGCAGGC ACTGGACTGG 
ATCCAAGAAA CAGGAGAATA TTACCTCTCA ACACATACCT CCACCGGAGA GACCACGGAG 
GAGACTCAGG AGCTGCTGAA AGAGTATGGA GAATTCAGGG TGCCTGCCAA GCAAACGAAG 
GAGAAGGTGA AACTTCTGAT CCAGCTAGCA GACAGCTTTG TGGAAAAGGG CCACATCCAC 
GCCACAGAGA TACGGAAATG GGTGACCACG GTGGACAAGC ACTACCGCGA TTTCTCCCTG 
AGGATGGGGA AGTACCGGTA CTCGCTGGAG AAGGCGCTGG GGGTCAACAC GGAGGATAAC 
AAGGACCTAG AGCTGGACAT CATCCCAGCA AGCCTTTCGG ATCGGGAGGT CAAGCTCCGG 
GACGCTAACC ATGAGATCAA TGAAGAGAAA CGGAAGTCAG CTCGAAAGAA AGAGTTCATT 
ATGGCTGAAC TGCTCCAAAC TGAGAAGGCT TATGTAAGGG ACTTGCACGA GTGCTTGGAG 
ACCTACCTGT GGGAGATGAC TAGTGGTGTG GAAGAGATAC CCCCCGGCAT CCTCAATAAG 
GAACATATCA TCTTCGGCAA CATCCAGGAG ATCTATGACT TCCATAACAA CATCTTCCTC 
AAAGAGCTGG AGAAGTACGA GCAGCTGCCT GAGGATGTGG GACACTGCTT TGTTACCTGG 
GCAGACAAAT TTCAGATGTA TGTCACCTAC TGTAAAAACA AGCCTGATTC CAACCAGCTG 
ATCCTGGAGC ATGCGGGCAC CTTCTTTGAT GAGATCCAGC AGCGGCATGG CCTGGCCAAC 
TCCATCTCCT CCTACCTAAT TAAGCCTGTC CAGAGGGTTA CCAAGTACCA GCTGCTCCTG 
AAGGAACTTC TAACTTGCTG TGAAGAAGGG AAAGGAGAGC TGAAGGATGG CCTGGAGGTG 
ATGCTCAGTG TCCCCAAGAA AGCCAACGAT GCCATGCATG TCAGCATGCT GGAAGGGTTC 
GATGAAAACT TGGATGTACA GGGGGAGTTG ATTCTCCAGG ATGCCTTTCA AGTGTGGGAC 
CCAAAGTCGC TGATCCGGAA GGGGCGGGAG CGCCATCTGT TCCTCTTTGA GATCTCCTTG 
GTGTTTAGCA AAGAGATCAA AGACTCTTCA GGACACACGA AATATGTTTA CAAGAACAAG 
CTACTGACCT CAGAGCTGGG TGTGACCGAG CATGTAGAAG GTGACCCCTG CAAATTCGCC 
TTGTGGTCTG GGCGCACCCC ATCCTCAGAC AATAAAACAG TGCTCAAAGC TTCCAACATC 
GAAACCAAGC AGGAATGGAT CAAGAACATT CGAGAAGTGA TTCAGGAGAG GATCATTCAT 
CTTAAAGGCG CTTTAAAGGA GCCAATTCAG CTCCCCAAAA CACCAGCCAA ACTGAGGAAC 
AATAGTAAGA GGGATGGGGT AGAGGATGGT GACAGCCAAG GGGATGGGAG CAGCCAGCCA 
GATACCATAT CCATTGCCTC CAGGACCTCT CAGAACACAG TGGAAAGTGA CAAGCTCTCA 
GGTGGATGTG AGCTGACCGT GGTCCTCCAG GACTTCAGCG CAGGCCACAG CAGCGAGCTG 
AGCATCCAGG TGGGCCAGAC GGTGGAGCTG CTGGAACGAC CGAGTGAGAG GCCTGGCTGG 
TGTCTGGTCC GCACCACAGA GCGGAGCCCC CCACAGGAAG GCCTGGTACC CAGCAGCGCC 
CTGTGCATCT CACACTCTCG CAGCAGCGTG GAAATGGACT GCTTCTTCCC CTTGAAAGAT 
TCATACTCTC ATTCCTCCAG TGAGAATGGA GGCAAATCCG AGTCCGTGGC CCACCTGCAG 
TCTCAGCCCT CCCTGAACTC CATCCACAGT TCCCCTGGTC CCAAGCGCTC CACCAACACC 
CTCAAAAAGT GGCTGACCAG CCCCGTGCGC CGCCTCAACA GCGGAAAAGC CGACGGGAAC 
ATCAAAAAGC AGAAGAAAGT CCGGGATGGG CGGAAGAGCT TCGATCTGGG ATCGCCCAAG 
CCTGGGGATG AGACGACACC TCAGGGAGAC AGCGCTGACG AGAAGAGCAA GAAGGGTTGG 
GGCGAAGACG AGCCAGATGA AGAGTCACAC ACACCCCTCC CTCCGCCCAT GAAGATCTTT 
GACAACGACC CCACACAGGA TGAGATGTCG TCTTTGCTAG CAGCCCGGCA GGCTCCCCCT 
GACGTGCCTA CTGCTGCAGA CCTTGTCAGT GCAATAGAAA AGTTGGTCAA AAATAAGCTG 
ACTCTGGAAG GAGGCTCTTA CCGGGGGAGC TTGAAGGACC CTACAGGCTG CCTGAATGAG 
GGGATGACCC CTCCCACACC TCCTAGAAAC CTGGAAGAAG AACAGAAAGC CAAGGCGCTG 
AGAGGCAGGA TGTTTGTCCT GAATGAGCTG GTACAGACAG AGAAGGACTA CGTCAAGGAC 
CTGGGGATTG TGGTGGAGGG CTTCATGAAG AGAATAGAAG AGAAGGGGGT CCCTGAGGAC 
ATGCGAGGGA AGGAAAAGAT CGTGTTTGGA AATATCCACC AGATTTACGA CTGGCATAAA 
GATTTTTTCC TGGCTGAACT GGAAAAATGT ATCCAAGAGC AAGACAGATT GGCCCAGCTC 
TTCATTAAAC ACGAGCGGAA GCTGCACATC TACGTGTGGT ACTGCCAGAA CAAGCCGCGT 
TCCGAGTACA TCGTGGCTGA GTACGATGCC TATTTTGAGG AGGTTAAACA AGAGATCAAT 
CAAAGGCTGA CCCTTAGTGA CTTCCTGATC AAGCCCATTC AGAGAATAAC AAAGTACCAG 
CTGCTACTCA AGGACTTCCT GCGATACAGC GAGAAGGCTG GTTTGGAGTG TTCCGATATT 
GAGAAAGCAG TGGAGTTAAT GTGCCTTGTC CCCAAGCGCT GTAATGACAT GATGAATCTG 
GGACGCCTGC AGGGCTTCGA GGGCACCCTG ACCGCCCAGG GCAAGCTACT GCAGCAGGAC 
ACATTCTATG TGATCGAGCT GGATGCCGGC ATGCAGTCCC GGACCAAGGA GAGGCGTGTG 
TTTCTCTTTG AGCAGATTGT CATCTTCAGT GAACTGCTCC GAAAGGGGTC CCTCACCCCA 
GGCTACATGT TCAAAAGAAG CATCAAGATG AATTACTTGG TCCTGGAAGA CAATGTGGAT 
GGTGACCCCT GCAAATTTGC ACTCATGAAC AGGGAGACTT CAGAAAGGGT CATTCTACAG 
GCTGCCAACT CAGACATCCA GCAGGCCTGG GTGCAGGACA TCAACCAAGT CCTAGAAACA 
CAGAGGGACT TCTTAAATGC ACTACAGTCA CCCATTGAGT ATCAGCGGAA GGAGAGAAGC 
ACAGCTGTGA TCCGGTCCCA GCCCCCGAGG GTTCCACAAG CCAGCCCCAG GCCCTACTCT 
TCTGGCCCGG TGGGCTCAGA GAAGCCCCCC AAGGGCTCCA GCTATAACCC GCCTCTGCCA 
CCCCTGAAGA TATCTACCTC TAATGGCAGT CCAGGGTTCG ACTACCATCA GCCTGGGGAC 
AAGTTTGACG CTAGCAAGCA GAACGACCTG GGAGGCTGCA ATGGGACATC AACCATGACT 
GTAATCAAAG ATTACTATGC ACTGAAGGAG AATGAAATCT GCGTGAGCCA AGGTGAGGTG 
GTACAGGTCC TCGCCGTCAA CCAGCAGAAC ATGTGTCTGG TGTACCAGCC TGCCAGCGAC 
CATTCCCCGG CAGCAGAGGG CTGGGTCCCA GGCAGCATCC TGGCGCCCCT CGCCAAAGCC 
ACAGCAGCGG CCGAAAGTAG TGACGGGAGC ATCAAGAAGT CATGCTCTTG GCACACTCTA 
CGCATGAGAA AGCGGGCAGA CGTGGAGAAC TCGGGTAAAA ATGAAGCCAC AGGGCCTCGT 
AAACCCAAGG ATATTCTGGG CAACAAAGTC TCTGTTAAAG AGACGAACAG TTCCGAGGAG 
TCAGAGTGTG ATGATCTTGA CCCTAATACT AGTATGGAGA TCTTGAATCC AAATTTCATC 
CAGGAAGTGG CTCCAGAATT CCTCGTGCCC TTAGTAGATG TGACCTGTTT GCTTGGAGAC 
ACAGTGCTAC TGCAGTGTAA AGCCTGTGGG CGGCCAAAGC CCAGCATCAC CTGGAAGGGT 
CCGGATCAGA ACATCCTAGA CACTGACAAC AGTTCAGCAA CATACACCAT CTCCTCCTGT 
GATTCTGGGG AAAGCACCCT CAAGATCTGC AACCTGATGC CCCAGGATAG TGGGATTTAT 
ACCTGCATAG CAGCAAACGA CCACGGGACT GCATCCACAT CTGCTACAGT TAAGGTGCAA 
GGTGTCCCCG CAGCCCCCAA CCGCCCAATC GCACAGGAGA GGAGCTGCAC CTCCGTCATC 
CTCCGCTGGC TGCCCCCAGC CAGCACCGGA AACTGCACTA TTTCGGGGTA CACAGTGGAG 
TATCGCGAGG AAGGTTCCCA GGTCTGGCAG CAATCCGTAG CTTCGACCTT GGACACTTAC 
CTGGTCATTG AAGACCTTAG TCCTGGGTGC CCTTATCAGT TCAGGGTCAG TGCCAGCAAC 
CCCTGGGGAA TCAGCCTTCC CAGTGAGCCC TCGGAGTTTG TGCGCCTTCC AGAGTACGAT 
GCAGCCGCCG ACGGTGCCAC CATTTCTTGG AAGGAAAATT TTGATTCTGC TTACACTGAA 
CTGAACGAGA TTGGCAGAGG CCGTTTCTCT ATTGTAAAGA AATGCATTCA CAAAGCTACC 
CGCAAAGATG TTGCTGTGAA ATTTGTGAGC AAAAAAATGA AGAAGAAGGA GCAGGCTGCT 
CACGAGGCCG CCCTGCTCCA GCACCTGCAG CACCCTCAGT ATGTCACGCT CCATGACACC 
TACGAGTCCC CCACATCCTA CATCCTGATT TTGGAACTGA TGGATGACGG CCGACTCTTG 
GACTACCTTA TGAATCATGA TGAGCTGATG GAAGAAAAGG TAGCTTTCTA CATTCGAGAC 
ATCATGGAAG CTCTGCAGTA CCTGCACAAC TGTAGGGTCG CACATTTGGA CATAAAGCCC 
GAAAACCTGC TCATTGACCT CCGGATCCCA GTGCCTCGAG TGAAGCTCAT CGACTTGGAA 
GACGCTGTCC AGATCTCAGG CCACTTCCAT ATCCACCATC TGCTGGGGAA CCCTGAGTTC 
GCTGCCCCCG AAGTGATCCA AGGCATCCCT GTCTCCCTGG GCACAGACAT CTGGAGCATC 
GGGGTTCTGA CATATGTCAT GCTTAGTGGG GTCTCCCCCT TCTTAGATGA GAGCAAAGAG 
GAAACCTGTA TCAACGTGTG CAGGGTGGAC TTTAGCTTCC CCCACGAGTA CTTCTGCGGT 
GTGAGCAATG CTGCCAGGGA TTTCATCAAC GTAATCTTAC AGGAAGACTT CCGGAGGCGG 
CCCACAGCAG CCACGTGCCT GCAGCATCCC TGGCTACAGC CCCACAACGG CAGCTACTCC 
AAGATCCCCT TGGACACCTC CCGTCTGGCC TGCTTCATAG AACGCCGCAA GCACCAGAAT 
GATGTGCGGC CCATTCCCAA TGTCAAGAGT TACATCGTCA ATAGGGTGAA CCAAGGGACC 
TCGCTATCCC ACAACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245956.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244258.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X8, mRNA.

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CloneID	OMu75312
Clone ID Related Accession (Same CDS sequence)	XM_011245956.2 , XM_011245956.3
Accession Version	XM_011245956.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245956.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGTC CCTCTTTCAT 
GCCACTTCCT TGCAGAAGAC GCACCAGAGC GCCCTGCAGG TACAGCAGAA GGCAGAGGCA 
CTGCTCCAGG CCGGCCACTA CGACGCAGAC GCCATCCGGG AATGTGCTGA GAAGGTGGCC 
CTCCACTGGC AACAGCTCAT GCTAAAGATG GAGGACCGGC TGAAGCTTGT CAACGCCTCT 
GTGGCCTTTT ACAAGACCTC TGAGCAGGTG TGCAGTGTTC TGGAGAGCTT GGAGCAGGAG 
TACCGGAGAG ACGAGGACTG GTGTGGTGGG AGAGACAAGC TGGGGCCGGC GGCAGAGATG 
GACCACGTTA TTCCACTCCT CAGCAAGCAC CTGGAGCAGA AGGAGGCCTT CCTGAAGGCC 
TGCACGCTGG CAAGGCGGAA CGCCGAGGTG TTCCTCAAAT ACATCCACAG GAACAATGTG 
AGCATGCCTA GTGTTGCCAG CCACACCCGG GGGCCTGAAC AGCAAGTAAA AGCCATCTTG 
AGCGAGCTCT TGCAGAGGGA GAATCGAGTC CTGCACTTCT GGACCTTGAA GAAGCGGCGG 
TTAGACCAGT GTCAGCAATA TGTGGTGTTT GAGCGCAGTG CTAAGCAGGC ACTGGACTGG 
ATCCAAGAAA CAGGAGAATA TTACCTCTCA ACACATACCT CCACCGGAGA GACCACGGAG 
GAGACTCAGG AGCTGCTGAA AGAGTATGGA GAATTCAGGG TGCCTGCCAA GCAAACGAAG 
GAGAAGGTGA AACTTCTGAT CCAGCTAGCA GACAGCTTTG TGGAAAAGGG CCACATCCAC 
GCCACAGAGA TACGGAAATG GGTGACCACG GTGGACAAGC ACTACCGCGA TTTCTCCCTG 
AGGATGGGGA AGTACCGGTA CTCGCTGGAG AAGGCGCTGG GGGTCAACAC GGAGGATAAC 
AAGGACCTAG AGCTGGACAT CATCCCAGCA AGCCTTTCGG ATCGGGAGGT CAAGCTCCGG 
GACGCTAACC ATGAGATCAA TGAAGAGAAA CGGAAGTCAG CTCGAAAGAA AGAGTTCATT 
ATGGCTGAAC TGCTCCAAAC TGAGAAGGCT TATGTAAGGG ACTTGCACGA GTGCTTGGAG 
ACCTACCTGT GGGAGATGAC TAGTGGTGTG GAAGAGATAC CCCCCGGCAT CCTCAATAAG 
GAACATATCA TCTTCGGCAA CATCCAGGAG ATCTATGACT TCCATAACAA CATCTTCCTC 
AAAGAGCTGG AGAAGTACGA GCAGCTGCCT GAGGATGTGG GACACTGCTT TGTTACCTGG 
GCAGACAAAT TTCAGATGTA TGTCACCTAC TGTAAAAACA AGCCTGATTC CAACCAGCTG 
ATCCTGGAGC ATGCGGGCAC CTTCTTTGAT GAGATCCAGC AGCGGCATGG CCTGGCCAAC 
TCCATCTCCT CCTACCTAAT TAAGCCTGTC CAGAGGGTTA CCAAGTACCA GCTGCTCCTG 
AAGGAACTTC TAACTTGCTG TGAAGAAGGG AAAGGAGAGC TGAAGGATGG CCTGGAGGTG 
ATGCTCAGTG TCCCCAAGAA AGCCAACGAT GCCATGCATG TCAGCATGCT GGAAGGGTTC 
GATGAAAACT TGGATGTACA GGGGGAGTTG ATTCTCCAGG ATGCCTTTCA AGTGTGGGAC 
CCAAAGTCGC TGATCCGGAA GGGGCGGGAG CGCCATCTGT TCCTCTTTGA GATCTCCTTG 
GTGTTTAGCA AAGAGATCAA AGACTCTTCA GGACACACGA AATATGTTTA CAAGAACAAG 
CTACTGACCT CAGAGCTGGG TGTGACCGAG CATGTAGAAG GTGACCCCTG CAAATTCGCC 
TTGTGGTCTG GGCGCACCCC ATCCTCAGAC AATAAAACAG TGCTCAAAGC TTCCAACATC 
GAAACCAAGC AGGAATGGAT CAAGAACATT CGAGAAGTGA TTCAGGAGAG GATCATTCAT 
CTTAAAGGCG CTTTAAAGGA GCCAATTCAG CTCCCCAAAA CACCAGCCAA ACTGAGGAAC 
AATAGTAAGA GGGATGGGGT AGAGGATGGT GACAGCCAAG GGGATGGGAG CAGCCAGCCA 
GATACCATAT CCATTGCCTC CAGGACCTCT CAGAACACAG TGGAAAGTGA CAAGCTCTCA 
GGTGGATGTG AGCTGACCGT GGTCCTCCAG GACTTCAGCG CAGGCCACAG CAGCGAGCTG 
AGCATCCAGG TGGGCCAGAC GGTGGAGCTG CTGGAACGAC CGAGTGAGAG GCCTGGCTGG 
TGTCTGGTCC GCACCACAGA GCGGAGCCCC CCACAGGAAG GCCTGGTACC CAGCAGCGCC 
CTGTGCATCT CACACTCTCG CAGCAGCGTG GAAATGGACT GCTTCTTCCC CTTGAAAGAT 
TCATACTCTC ATTCCTCCAG TGAGAATGGA GGCAAATCCG AGTCCGTGGC CCACCTGCAG 
TCTCAGCCCT CCCTGAACTC CATCCACAGT TCCCCTGGTC CCAAGCGCTC CACCAACACC 
CTCAAAAAGT GGCTGACCAG CCCCGTGCGC CGCCTCAACA GCGGAAAAGC CGACGGGAAC 
ATCAAAAAGC AGAAGAAAGT CCGGGATGGG CGGAAGAGCT TCGATCTGGG ATCGCCCAAG 
CCTGGGGATG AGACGACACC TCAGGGAGAC AGCGCTGACG AGAAGAGCAA GAAGGGTTGG 
GGCGAAGACG AGCCAGATGA AGAGTCACAC ACACCCCTCC CTCCGCCCAT GAAGATCTTT 
GACAACGACC CCACACAGGA TGAGATGTCG TCTTTGCTAG CAGCCCGGCA GGCTCCCCCT 
GACGTGCCTA CTGCTGCAGA CCTTGTCAGT GCAATAGAAA AGTTGGTCAA AAATAAGCTG 
ACTCTGGAAG GAGGCTCTTA CCGGGGGAGC TTGAAGGACC CTACAGGCTG CCTGAATGAG 
GGGATGACCC CTCCCACACC TCCTAGAAAC CTGGAAGAAG AACAGAAAGC CAAGGCGCTG 
AGAGGCAGGA TGTTTGTCCT GAATGAGCTG GTACAGACAG AGAAGGACTA CGTCAAGGAC 
CTGGGGATTG TGGTGGAGGG CTTCATGAAG AGAATAGAAG AGAAGGGGGT CCCTGAGGAC 
ATGCGAGGGA AGGAAAAGAT CGTGTTTGGA AATATCCACC AGATTTACGA CTGGCATAAA 
GATTTTTTCC TGGCTGAACT GGAAAAATGT ATCCAAGAGC AAGACAGATT GGCCCAGCTC 
TTCATTAAAC ACGAGCGGAA GCTGCACATC TACGTGTGGT ACTGCCAGAA CAAGCCGCGT 
TCCGAGTACA TCGTGGCTGA GTACGATGCC TATTTTGAGG AGGTTAAACA AGAGATCAAT 
CAAAGGCTGA CCCTTAGTGA CTTCCTGATC AAGCCCATTC AGAGAATAAC AAAGTACCAG 
CTGCTACTCA AGGACTTCCT GCGATACAGC GAGAAGGCTG GTTTGGAGTG TTCCGATATT 
GAGAAAGCAG TGGAGTTAAT GTGCCTTGTC CCCAAGCGCT GTAATGACAT GATGAATCTG 
GGACGCCTGC AGGGCTTCGA GGGCACCCTG ACCGCCCAGG GCAAGCTACT GCAGCAGGAC 
ACATTCTATG TGATCGAGCT GGATGCCGGC ATGCAGTCCC GGACCAAGGA GAGGCGTGTG 
TTTCTCTTTG AGCAGATTGT CATCTTCAGT GAACTGCTCC GAAAGGGGTC CCTCACCCCA 
GGCTACATGT TCAAAAGAAG CATCAAGATG AATTACTTGG TCCTGGAAGA CAATGTGGAT 
GGTGACCCCT GCAAATTTGC ACTCATGAAC AGGGAGACTT CAGAAAGGGT CATTCTACAG 
GCTGCCAACT CAGACATCCA GCAGGCCTGG GTGCAGGACA TCAACCAAGT CCTAGAAACA 
CAGAGGGACT TCTTAAATGC ACTACAGTCA CCCATTGAGT ATCAGCGGAA GGAGAGAAGC 
ACAGCTGTGA TCCGGTCCCA GCCCCCGAGG GTTCCACAAG CCAGCCCCAG GCCCTACTCT 
TCTGGCCCGG TGGGCTCAGA GAAGCCCCCC AAGGGCTCCA GCTATAACCC GCCTCTGCCA 
CCCCTGAAGA TATCTACCTC TAATGGCAGT CCAGGGTTCG ACTACCATCA GCCTGGGGAC 
AAGTTTGACG CTAGCAAGCA GAACGACCTG GGAGGCTGCA ATGGGACATC AACCATGACT 
GTAATCAAAG ATTACTATGC ACTGAAGGAG AATGAAATCT GCGTGAGCCA AGGTGAGGTG 
GTACAGGTCC TCGCCGTCAA CCAGCAGAAC ATGTGTCTGG TGTACCAGCC TGCCAGCGAC 
CATTCCCCGG CAGCAGAGGG CTGGGTCCCA GGCAGCATCC TGGCGCCCCT CGCCAAAGCC 
ACAGCAGCGG CCGAAAGTAG TGACGGGAGC ATCAAGAAGT CATGCTCTTG GCACACTCTA 
CGCATGAGAA AGCGGGCAGA CGTGGAGAAC TCGGGTAAAA ATGAAGCCAC AGGGCCTCGT 
AAACCCAAGG ATATTCTGGG CAACAAAGTC TCTGTTAAAG AGACGAACAG TTCCGAGGAG 
TCAGAGTGTG ATGATCTTGA CCCTAATACT AGTATGGAGA TCTTGAATCC AAATTTCATC 
CAGGAAGTGG CTCCAGAATT CCTCGTGCCC TTAGTAGATG TGACCTGTTT GCTTGGAGAC 
ACAGTGCTAC TGCAGTGTAA AGCCTGTGGG CGGCCAAAGC CCAGCATCAC CTGGAAGGGT 
CCGGATCAGA ACATCCTAGA CACTGACAAC AGTTCAGCAA CATACACCAT CTCCTCCTGT 
GATTCTGGGG AAAGCACCCT CAAGATCTGC AACCTGATGC CCCAGGATAG TGGGATTTAT 
ACCTGCATAG CAGCAAACGA CCACGGGACT GCATCCACAT CTGCTACAGT TAAGGTGCAA 
GGTGTCCCCG CAGCCCCCAA CCGCCCAATC GCACAGGAGA GGAGCTGCAC CTCCGTCATC 
CTCCGCTGGC TGCCCCCAGC CAGCACCGGA AACTGCACTA TTTCGGGGTA CACAGTGGAG 
TATCGCGAGG AAGGTTCCCA GGTCTGGCAG CAATCCGTAG CTTCGACCTT GGACACTTAC 
CTGGTCATTG AAGACCTTAG TCCTGGGTGC CCTTATCAGT TCAGGGTCAG TGCCAGCAAC 
CCCTGGGGAA TCAGCCTTCC CAGTGAGCCC TCGGAGTTTG TGCGCCTTCC AGAGTACGAT 
GCAGCCGCCG ACGGTGCCAC CATTTCTTGG AAGGAAAATT TTGATTCTGC TTACACTGAA 
CTGAACGAGA TTGGCAGAGG CCGTTTCTCT ATTGTAAAGA AATGCATTCA CAAAGCTACC 
CGCAAAGATG TTGCTGTGAA ATTTGTGAGC AAAAAAATGA AGAAGAAGGA GCAGGCTGCT 
CACGAGGCCG CCCTGCTCCA GCACCTGCAG CACCCTCAGT ATGTCACGCT CCATGACACC 
TACGAGTCCC CCACATCCTA CATCCTGATT TTGGAACTGA TGGATGACGG CCGACTCTTG 
GACTACCTTA TGAATCATGA TGAGCTGATG GAAGAAAAGG TAGCTTTCTA CATTCGAGAC 
ATCATGGAAG CTCTGCAGTA CCTGCACAAC TGTAGGGTCG CACATTTGGA CATAAAGCCC 
GAAAACCTGC TCATTGACCT CCGGATCCCA GTGCCTCGAG TGAAGCTCAT CGACTTGGAA 
GACGCTGTCC AGATCTCAGG CCACTTCCAT ATCCACCATC TGCTGGGGAA CCCTGAGTTC 
GCTGCCCCCG AAGTGATCCA AGGCATCCCT GTCTCCCTGG GCACAGACAT CTGGAGCATC 
GGGGTTCTGA CATATGTCAT GCTTAGTGGG GTCTCCCCCT TCTTAGATGA GAGCAAAGAG 
GAAACCTGTA TCAACGTGTG CAGGGTGGAC TTTAGCTTCC CCCACGAGTA CTTCTGCGGT 
GTGAGCAATG CTGCCAGGGA TTTCATCAAC GTAATCTTAC AGGAAGACTT CCGGAGGCGG 
CCCACAGCAG CCACGTGCCT GCAGCATCCC TGGCTACAGC CCCACAACGG CAGCTACTCC 
AAGATCCCCT TGGACACCTC CCGTCTGGCC TGCTTCATAG AACGCCGCAA GCACCAGAAT 
GATGTGCGGC CCATTCCCAA TGTCAAGAGT TACATCGTCA ATAGGGTGAA CCAAGGGACC 
TCGCTATCCC ACAACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244258.1
CDS	24875..33835
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245956.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245956.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GGACTCAGCG 
ATGTCCAACA ACAAGACACC CCACAGTAGC TCTATCAGCC ATATTGAGTC TGTCCTCCAG 
CAGCTGGACG ATGCTCAGGT ACAGATGGAA GAGCTGTTTC ACGAGCGGAA GATCAAGCTG 
GACATCTTCC TACAGCTGCG CATCTTTGAA CAGTACACCA TTGAGGTCAC TGCAGAGCTG 
GATGCCTGGA ACGAGGACTT GCTCCGGCAG ATGAATGATT TCAACACAGA GGATCTGACC 
CTGGCTGAGC AGCGGCTACA GCGCCACACA GAACGGAAGC TTGCCATGAA CAACATGACG 
TTCGAGGTCA TCCAGCAGGG CCAGGACCTG CACCAGTACA TCATGGAAGT GCAAGCGTCA 
GGAATCGAGT TAATCTGTGA GAAGGACCTT GACCTGGCAG CTCAGGTGCA GGAGCTGCTG 
GAGTTTCTCC ATGAGAAGCA GCACGAGCTG GAGCTCAATG CGGAGCAGAC GCACAAGCGG 
CTAGAGCAGT GTCTCCAGCT GAGGCACCTC CAGGCTGAGG TCAAACAGGT TCTGGGATGG 
ATCCGCAATG GAGAGTCGAT GCTCAACGCC AGCCTGGTCA ACGCCAGCTC TTTGTCGGAG 
GCAGAGCAGC TGCAGCGGGA GCATGAACAG TTCCAGCTAG CCATCGAGTC CCTCTTTCAT 
GCCACTTCCT TGCAGAAGAC GCACCAGAGC GCCCTGCAGG TACAGCAGAA GGCAGAGGCA 
CTGCTCCAGG CCGGCCACTA CGACGCAGAC GCCATCCGGG AATGTGCTGA GAAGGTGGCC 
CTCCACTGGC AACAGCTCAT GCTAAAGATG GAGGACCGGC TGAAGCTTGT CAACGCCTCT 
GTGGCCTTTT ACAAGACCTC TGAGCAGGTG TGCAGTGTTC TGGAGAGCTT GGAGCAGGAG 
TACCGGAGAG ACGAGGACTG GTGTGGTGGG AGAGACAAGC TGGGGCCGGC GGCAGAGATG 
GACCACGTTA TTCCACTCCT CAGCAAGCAC CTGGAGCAGA AGGAGGCCTT CCTGAAGGCC 
TGCACGCTGG CAAGGCGGAA CGCCGAGGTG TTCCTCAAAT ACATCCACAG GAACAATGTG 
AGCATGCCTA GTGTTGCCAG CCACACCCGG GGGCCTGAAC AGCAAGTAAA AGCCATCTTG 
AGCGAGCTCT TGCAGAGGGA GAATCGAGTC CTGCACTTCT GGACCTTGAA GAAGCGGCGG 
TTAGACCAGT GTCAGCAATA TGTGGTGTTT GAGCGCAGTG CTAAGCAGGC ACTGGACTGG 
ATCCAAGAAA CAGGAGAATA TTACCTCTCA ACACATACCT CCACCGGAGA GACCACGGAG 
GAGACTCAGG AGCTGCTGAA AGAGTATGGA GAATTCAGGG TGCCTGCCAA GCAAACGAAG 
GAGAAGGTGA AACTTCTGAT CCAGCTAGCA GACAGCTTTG TGGAAAAGGG CCACATCCAC 
GCCACAGAGA TACGGAAATG GGTGACCACG GTGGACAAGC ACTACCGCGA TTTCTCCCTG 
AGGATGGGGA AGTACCGGTA CTCGCTGGAG AAGGCGCTGG GGGTCAACAC GGAGGATAAC 
AAGGACCTAG AGCTGGACAT CATCCCAGCA AGCCTTTCGG ATCGGGAGGT CAAGCTCCGG 
GACGCTAACC ATGAGATCAA TGAAGAGAAA CGGAAGTCAG CTCGAAAGAA AGAGTTCATT 
ATGGCTGAAC TGCTCCAAAC TGAGAAGGCT TATGTAAGGG ACTTGCACGA GTGCTTGGAG 
ACCTACCTGT GGGAGATGAC TAGTGGTGTG GAAGAGATAC CCCCCGGCAT CCTCAATAAG 
GAACATATCA TCTTCGGCAA CATCCAGGAG ATCTATGACT TCCATAACAA CATCTTCCTC 
AAAGAGCTGG AGAAGTACGA GCAGCTGCCT GAGGATGTGG GACACTGCTT TGTTACCTGG 
GCAGACAAAT TTCAGATGTA TGTCACCTAC TGTAAAAACA AGCCTGATTC CAACCAGCTG 
ATCCTGGAGC ATGCGGGCAC CTTCTTTGAT GAGATCCAGC AGCGGCATGG CCTGGCCAAC 
TCCATCTCCT CCTACCTAAT TAAGCCTGTC CAGAGGGTTA CCAAGTACCA GCTGCTCCTG 
AAGGAACTTC TAACTTGCTG TGAAGAAGGG AAAGGAGAGC TGAAGGATGG CCTGGAGGTG 
ATGCTCAGTG TCCCCAAGAA AGCCAACGAT GCCATGCATG TCAGCATGCT GGAAGGGTTC 
GATGAAAACT TGGATGTACA GGGGGAGTTG ATTCTCCAGG ATGCCTTTCA AGTGTGGGAC 
CCAAAGTCGC TGATCCGGAA GGGGCGGGAG CGCCATCTGT TCCTCTTTGA GATCTCCTTG 
GTGTTTAGCA AAGAGATCAA AGACTCTTCA GGACACACGA AATATGTTTA CAAGAACAAG 
CTACTGACCT CAGAGCTGGG TGTGACCGAG CATGTAGAAG GTGACCCCTG CAAATTCGCC 
TTGTGGTCTG GGCGCACCCC ATCCTCAGAC AATAAAACAG TGCTCAAAGC TTCCAACATC 
GAAACCAAGC AGGAATGGAT CAAGAACATT CGAGAAGTGA TTCAGGAGAG GATCATTCAT 
CTTAAAGGCG CTTTAAAGGA GCCAATTCAG CTCCCCAAAA CACCAGCCAA ACTGAGGAAC 
AATAGTAAGA GGGATGGGGT AGAGGATGGT GACAGCCAAG GGGATGGGAG CAGCCAGCCA 
GATACCATAT CCATTGCCTC CAGGACCTCT CAGAACACAG TGGAAAGTGA CAAGCTCTCA 
GGTGGATGTG AGCTGACCGT GGTCCTCCAG GACTTCAGCG CAGGCCACAG CAGCGAGCTG 
AGCATCCAGG TGGGCCAGAC GGTGGAGCTG CTGGAACGAC CGAGTGAGAG GCCTGGCTGG 
TGTCTGGTCC GCACCACAGA GCGGAGCCCC CCACAGGAAG GCCTGGTACC CAGCAGCGCC 
CTGTGCATCT CACACTCTCG CAGCAGCGTG GAAATGGACT GCTTCTTCCC CTTGAAAGAT 
TCATACTCTC ATTCCTCCAG TGAGAATGGA GGCAAATCCG AGTCCGTGGC CCACCTGCAG 
TCTCAGCCCT CCCTGAACTC CATCCACAGT TCCCCTGGTC CCAAGCGCTC CACCAACACC 
CTCAAAAAGT GGCTGACCAG CCCCGTGCGC CGCCTCAACA GCGGAAAAGC CGACGGGAAC 
ATCAAAAAGC AGAAGAAAGT CCGGGATGGG CGGAAGAGCT TCGATCTGGG ATCGCCCAAG 
CCTGGGGATG AGACGACACC TCAGGGAGAC AGCGCTGACG AGAAGAGCAA GAAGGGTTGG 
GGCGAAGACG AGCCAGATGA AGAGTCACAC ACACCCCTCC CTCCGCCCAT GAAGATCTTT 
GACAACGACC CCACACAGGA TGAGATGTCG TCTTTGCTAG CAGCCCGGCA GGCTCCCCCT 
GACGTGCCTA CTGCTGCAGA CCTTGTCAGT GCAATAGAAA AGTTGGTCAA AAATAAGCTG 
ACTCTGGAAG GAGGCTCTTA CCGGGGGAGC TTGAAGGACC CTACAGGCTG CCTGAATGAG 
GGGATGACCC CTCCCACACC TCCTAGAAAC CTGGAAGAAG AACAGAAAGC CAAGGCGCTG 
AGAGGCAGGA TGTTTGTCCT GAATGAGCTG GTACAGACAG AGAAGGACTA CGTCAAGGAC 
CTGGGGATTG TGGTGGAGGG CTTCATGAAG AGAATAGAAG AGAAGGGGGT CCCTGAGGAC 
ATGCGAGGGA AGGAAAAGAT CGTGTTTGGA AATATCCACC AGATTTACGA CTGGCATAAA 
GATTTTTTCC TGGCTGAACT GGAAAAATGT ATCCAAGAGC AAGACAGATT GGCCCAGCTC 
TTCATTAAAC ACGAGCGGAA GCTGCACATC TACGTGTGGT ACTGCCAGAA CAAGCCGCGT 
TCCGAGTACA TCGTGGCTGA GTACGATGCC TATTTTGAGG AGGTTAAACA AGAGATCAAT 
CAAAGGCTGA CCCTTAGTGA CTTCCTGATC AAGCCCATTC AGAGAATAAC AAAGTACCAG 
CTGCTACTCA AGGACTTCCT GCGATACAGC GAGAAGGCTG GTTTGGAGTG TTCCGATATT 
GAGAAAGCAG TGGAGTTAAT GTGCCTTGTC CCCAAGCGCT GTAATGACAT GATGAATCTG 
GGACGCCTGC AGGGCTTCGA GGGCACCCTG ACCGCCCAGG GCAAGCTACT GCAGCAGGAC 
ACATTCTATG TGATCGAGCT GGATGCCGGC ATGCAGTCCC GGACCAAGGA GAGGCGTGTG 
TTTCTCTTTG AGCAGATTGT CATCTTCAGT GAACTGCTCC GAAAGGGGTC CCTCACCCCA 
GGCTACATGT TCAAAAGAAG CATCAAGATG AATTACTTGG TCCTGGAAGA CAATGTGGAT 
GGTGACCCCT GCAAATTTGC ACTCATGAAC AGGGAGACTT CAGAAAGGGT CATTCTACAG 
GCTGCCAACT CAGACATCCA GCAGGCCTGG GTGCAGGACA TCAACCAAGT CCTAGAAACA 
CAGAGGGACT TCTTAAATGC ACTACAGTCA CCCATTGAGT ATCAGCGGAA GGAGAGAAGC 
ACAGCTGTGA TCCGGTCCCA GCCCCCGAGG GTTCCACAAG CCAGCCCCAG GCCCTACTCT 
TCTGGCCCGG TGGGCTCAGA GAAGCCCCCC AAGGGCTCCA GCTATAACCC GCCTCTGCCA 
CCCCTGAAGA TATCTACCTC TAATGGCAGT CCAGGGTTCG ACTACCATCA GCCTGGGGAC 
AAGTTTGACG CTAGCAAGCA GAACGACCTG GGAGGCTGCA ATGGGACATC AACCATGACT 
GTAATCAAAG ATTACTATGC ACTGAAGGAG AATGAAATCT GCGTGAGCCA AGGTGAGGTG 
GTACAGGTCC TCGCCGTCAA CCAGCAGAAC ATGTGTCTGG TGTACCAGCC TGCCAGCGAC 
CATTCCCCGG CAGCAGAGGG CTGGGTCCCA GGCAGCATCC TGGCGCCCCT CGCCAAAGCC 
ACAGCAGCGG CCGAAAGTAG TGACGGGAGC ATCAAGAAGT CATGCTCTTG GCACACTCTA 
CGCATGAGAA AGCGGGCAGA CGTGGAGAAC TCGGGTAAAA ATGAAGCCAC AGGGCCTCGT 
AAACCCAAGG ATATTCTGGG CAACAAAGTC TCTGTTAAAG AGACGAACAG TTCCGAGGAG 
TCAGAGTGTG ATGATCTTGA CCCTAATACT AGTATGGAGA TCTTGAATCC AAATTTCATC 
CAGGAAGTGG CTCCAGAATT CCTCGTGCCC TTAGTAGATG TGACCTGTTT GCTTGGAGAC 
ACAGTGCTAC TGCAGTGTAA AGCCTGTGGG CGGCCAAAGC CCAGCATCAC CTGGAAGGGT 
CCGGATCAGA ACATCCTAGA CACTGACAAC AGTTCAGCAA CATACACCAT CTCCTCCTGT 
GATTCTGGGG AAAGCACCCT CAAGATCTGC AACCTGATGC CCCAGGATAG TGGGATTTAT 
ACCTGCATAG CAGCAAACGA CCACGGGACT GCATCCACAT CTGCTACAGT TAAGGTGCAA 
GGTGTCCCCG CAGCCCCCAA CCGCCCAATC GCACAGGAGA GGAGCTGCAC CTCCGTCATC 
CTCCGCTGGC TGCCCCCAGC CAGCACCGGA AACTGCACTA TTTCGGGGTA CACAGTGGAG 
TATCGCGAGG AAGGTTCCCA GGTCTGGCAG CAATCCGTAG CTTCGACCTT GGACACTTAC 
CTGGTCATTG AAGACCTTAG TCCTGGGTGC CCTTATCAGT TCAGGGTCAG TGCCAGCAAC 
CCCTGGGGAA TCAGCCTTCC CAGTGAGCCC TCGGAGTTTG TGCGCCTTCC AGAGTACGAT 
GCAGCCGCCG ACGGTGCCAC CATTTCTTGG AAGGAAAATT TTGATTCTGC TTACACTGAA 
CTGAACGAGA TTGGCAGAGG CCGTTTCTCT ATTGTAAAGA AATGCATTCA CAAAGCTACC 
CGCAAAGATG TTGCTGTGAA ATTTGTGAGC AAAAAAATGA AGAAGAAGGA GCAGGCTGCT 
CACGAGGCCG CCCTGCTCCA GCACCTGCAG CACCCTCAGT ATGTCACGCT CCATGACACC 
TACGAGTCCC CCACATCCTA CATCCTGATT TTGGAACTGA TGGATGACGG CCGACTCTTG 
GACTACCTTA TGAATCATGA TGAGCTGATG GAAGAAAAGG TAGCTTTCTA CATTCGAGAC 
ATCATGGAAG CTCTGCAGTA CCTGCACAAC TGTAGGGTCG CACATTTGGA CATAAAGCCC 
GAAAACCTGC TCATTGACCT CCGGATCCCA GTGCCTCGAG TGAAGCTCAT CGACTTGGAA 
GACGCTGTCC AGATCTCAGG CCACTTCCAT ATCCACCATC TGCTGGGGAA CCCTGAGTTC 
GCTGCCCCCG AAGTGATCCA AGGCATCCCT GTCTCCCTGG GCACAGACAT CTGGAGCATC 
GGGGTTCTGA CATATGTCAT GCTTAGTGGG GTCTCCCCCT TCTTAGATGA GAGCAAAGAG 
GAAACCTGTA TCAACGTGTG CAGGGTGGAC TTTAGCTTCC CCCACGAGTA CTTCTGCGGT 
GTGAGCAATG CTGCCAGGGA TTTCATCAAC GTAATCTTAC AGGAAGACTT CCGGAGGCGG 
CCCACAGCAG CCACGTGCCT GCAGCATCCC TGGCTACAGC CCCACAACGG CAGCTACTCC 
AAGATCCCCT TGGACACCTC CCGTCTGGCC TGCTTCATAG AACGCCGCAA GCACCAGAAT 
GATGTGCGGC CCATTCCCAA TGTCAAGAGT TACATCGTCA ATAGGGTGAA CCAAGGGACC 
TCGCTATCCC ACAACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245957.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244259.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X9, mRNA.

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CloneID	OMu75313
Clone ID Related Accession (Same CDS sequence)	XM_011245957.2 , XM_011245957.3
Accession Version	XM_011245957.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAGAAGTC ATGCTCTTGG CACACTCTAC GCATGAGAAA GCGGGCAGAC 
GTGGAGAACT CGGGTAAAAA TGAAGCCACA GGGCCTCGTA AACCCAAGGA TATTCTGGGC 
AACAAAGTCT CTGTTAAAAT CTTGAATCCA AATTTCATCC AGGAAGTGGC TCCAGAATTC 
CTCGTGCCCT TAGTAGATGT GACCTGTTTG CTTGGAGACA CAGTGCTACT GCAGTGTAAA 
GCCTGTGGGC GGCCAAAGCC CAGCATCACC TGGAAGGGTC CGGATCAGAA CATCCTAGAC 
ACTGACAACA GTTCAGCAAC ATACACCATC TCCTCCTGTG ATTCTGGGGA AAGCACCCTC 
AAGATCTGCA ACCTGATGCC CCAGGATAGT GGGATTTATA CCTGCATAGC AGCAAACGAC 
CACGGGACTG CATCCACATC TGCTACAGTT AAGGTGCAAG GTGTCCCCGC AGCCCCCAAC 
CGCCCAATCG CACAGGAGAG GAGCTGCACC TCCGTCATCC TCCGCTGGCT GCCCCCAGCC 
AGCACCGGAA ACTGCACTAT TTCGGGGTAC ACAGTGGAGT ATCGCGAGGA AGGTTCCCAG 
GTCTGGCAGC AATCCGTAGC TTCGACCTTG GACACTTACC TGGTCATTGA AGACCTTAGT 
CCTGGGTGCC CTTATCAGTT CAGGGTCAGT GCCAGCAACC CCTGGGGAAT CAGCCTTCCC 
AGTGAGCCCT CGGAGTTTGT GCGCCTTCCA GAGTACGATG CAGCCGCCGA CGGTGCCACC 
ATTTCTTGGA AGGAAAATTT TGATTCTGCT TACACTGAAC TGAACGAGAT TGGCAGAGGC 
CGTTTCTCTA TTGTAAAGAA ATGCATTCAC AAAGCTACCC GCAAAGATGT TGCTGTGAAA 
TTTGTGAGCA AAAAAATGAA GAAGAAGGAG CAGGCTGCTC ACGAGGCCGC CCTGCTCCAG 
CACCTGCAGC ACCCTCAGTA TGTCACGCTC CATGACACCT ACGAGTCCCC CACATCCTAC 
ATCCTGATTT TGGAACTGAT GGATGACGGC CGACTCTTGG ACTACCTTAT GAATCATGAT 
GAGCTGATGG AAGAAAAGGT AGCTTTCTAC ATTCGAGACA TCATGGAAGC TCTGCAGTAC 
CTGCACAACT GTAGGGTCGC ACATTTGGAC ATAAAGCCCG AAAACCTGCT CATTGACCTC 
CGGATCCCAG TGCCTCGAGT GAAGCTCATC GACTTGGAAG ACGCTGTCCA GATCTCAGGC 
CACTTCCATA TCCACCATCT GCTGGGGAAC CCTGAGTTCG CTGCCCCCGA AGTGATCCAA 
GGCATCCCTG TCTCCCTGGG CACAGACATC TGGAGCATCG GGGTTCTGAC ATATGTCATG 
CTTAGTGGGG TCTCCCCCTT CTTAGATGAG AGCAAAGAGG AAACCTGTAT CAACGTGTGC 
AGGGTGGACT TTAGCTTCCC CCACGAGTAC TTCTGCGGTG TGAGCAATGC TGCCAGGGAT 
TTCATCAACG TAATCTTACA GGAAGACTTC CGGAGGCGGC CCACAGCAGC CACGTGCCTG 
CAGCATCCCT GGCTACAGCC CCACAACGGC AGCTACTCCA AGATCCCCTT GGACACCTCC 
CGTCTGGCCT GCTTCATAGA ACGCCGCAAG CACCAGAATG ATGTGCGGCC CATTCCCAAT 
GTCAAGAGTT ACATCGTCAA TAGGGTGAAC CAAGGGACCT CGCTATCCCA CAACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244259.1
CDS	4891..13830
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245957.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245957.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAGAAGTC ATGCTCTTGG CACACTCTAC GCATGAGAAA GCGGGCAGAC 
GTGGAGAACT CGGGTAAAAA TGAAGCCACA GGGCCTCGTA AACCCAAGGA TATTCTGGGC 
AACAAAGTCT CTGTTAAAAT CTTGAATCCA AATTTCATCC AGGAAGTGGC TCCAGAATTC 
CTCGTGCCCT TAGTAGATGT GACCTGTTTG CTTGGAGACA CAGTGCTACT GCAGTGTAAA 
GCCTGTGGGC GGCCAAAGCC CAGCATCACC TGGAAGGGTC CGGATCAGAA CATCCTAGAC 
ACTGACAACA GTTCAGCAAC ATACACCATC TCCTCCTGTG ATTCTGGGGA AAGCACCCTC 
AAGATCTGCA ACCTGATGCC CCAGGATAGT GGGATTTATA CCTGCATAGC AGCAAACGAC 
CACGGGACTG CATCCACATC TGCTACAGTT AAGGTGCAAG GTGTCCCCGC AGCCCCCAAC 
CGCCCAATCG CACAGGAGAG GAGCTGCACC TCCGTCATCC TCCGCTGGCT GCCCCCAGCC 
AGCACCGGAA ACTGCACTAT TTCGGGGTAC ACAGTGGAGT ATCGCGAGGA AGGTTCCCAG 
GTCTGGCAGC AATCCGTAGC TTCGACCTTG GACACTTACC TGGTCATTGA AGACCTTAGT 
CCTGGGTGCC CTTATCAGTT CAGGGTCAGT GCCAGCAACC CCTGGGGAAT CAGCCTTCCC 
AGTGAGCCCT CGGAGTTTGT GCGCCTTCCA GAGTACGATG CAGCCGCCGA CGGTGCCACC 
ATTTCTTGGA AGGAAAATTT TGATTCTGCT TACACTGAAC TGAACGAGAT TGGCAGAGGC 
CGTTTCTCTA TTGTAAAGAA ATGCATTCAC AAAGCTACCC GCAAAGATGT TGCTGTGAAA 
TTTGTGAGCA AAAAAATGAA GAAGAAGGAG CAGGCTGCTC ACGAGGCCGC CCTGCTCCAG 
CACCTGCAGC ACCCTCAGTA TGTCACGCTC CATGACACCT ACGAGTCCCC CACATCCTAC 
ATCCTGATTT TGGAACTGAT GGATGACGGC CGACTCTTGG ACTACCTTAT GAATCATGAT 
GAGCTGATGG AAGAAAAGGT AGCTTTCTAC ATTCGAGACA TCATGGAAGC TCTGCAGTAC 
CTGCACAACT GTAGGGTCGC ACATTTGGAC ATAAAGCCCG AAAACCTGCT CATTGACCTC 
CGGATCCCAG TGCCTCGAGT GAAGCTCATC GACTTGGAAG ACGCTGTCCA GATCTCAGGC 
CACTTCCATA TCCACCATCT GCTGGGGAAC CCTGAGTTCG CTGCCCCCGA AGTGATCCAA 
GGCATCCCTG TCTCCCTGGG CACAGACATC TGGAGCATCG GGGTTCTGAC ATATGTCATG 
CTTAGTGGGG TCTCCCCCTT CTTAGATGAG AGCAAAGAGG AAACCTGTAT CAACGTGTGC 
AGGGTGGACT TTAGCTTCCC CCACGAGTAC TTCTGCGGTG TGAGCAATGC TGCCAGGGAT 
TTCATCAACG TAATCTTACA GGAAGACTTC CGGAGGCGGC CCACAGCAGC CACGTGCCTG 
CAGCATCCCT GGCTACAGCC CCACAACGGC AGCTACTCCA AGATCCCCTT GGACACCTCC 
CGTCTGGCCT GCTTCATAGA ACGCCGCAAG CACCAGAATG ATGTGCGGCC CATTCCCAAT 
GTCAAGAGTT ACATCGTCAA TAGGGTGAAC CAAGGGACCT CGCTATCCCA CAACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245957.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244259.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X9, mRNA.

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CloneID	OMu75313
Clone ID Related Accession (Same CDS sequence)	XM_011245957.2 , XM_011245957.3
Accession Version	XM_011245957.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245957.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAGAAGTC ATGCTCTTGG CACACTCTAC GCATGAGAAA GCGGGCAGAC 
GTGGAGAACT CGGGTAAAAA TGAAGCCACA GGGCCTCGTA AACCCAAGGA TATTCTGGGC 
AACAAAGTCT CTGTTAAAAT CTTGAATCCA AATTTCATCC AGGAAGTGGC TCCAGAATTC 
CTCGTGCCCT TAGTAGATGT GACCTGTTTG CTTGGAGACA CAGTGCTACT GCAGTGTAAA 
GCCTGTGGGC GGCCAAAGCC CAGCATCACC TGGAAGGGTC CGGATCAGAA CATCCTAGAC 
ACTGACAACA GTTCAGCAAC ATACACCATC TCCTCCTGTG ATTCTGGGGA AAGCACCCTC 
AAGATCTGCA ACCTGATGCC CCAGGATAGT GGGATTTATA CCTGCATAGC AGCAAACGAC 
CACGGGACTG CATCCACATC TGCTACAGTT AAGGTGCAAG GTGTCCCCGC AGCCCCCAAC 
CGCCCAATCG CACAGGAGAG GAGCTGCACC TCCGTCATCC TCCGCTGGCT GCCCCCAGCC 
AGCACCGGAA ACTGCACTAT TTCGGGGTAC ACAGTGGAGT ATCGCGAGGA AGGTTCCCAG 
GTCTGGCAGC AATCCGTAGC TTCGACCTTG GACACTTACC TGGTCATTGA AGACCTTAGT 
CCTGGGTGCC CTTATCAGTT CAGGGTCAGT GCCAGCAACC CCTGGGGAAT CAGCCTTCCC 
AGTGAGCCCT CGGAGTTTGT GCGCCTTCCA GAGTACGATG CAGCCGCCGA CGGTGCCACC 
ATTTCTTGGA AGGAAAATTT TGATTCTGCT TACACTGAAC TGAACGAGAT TGGCAGAGGC 
CGTTTCTCTA TTGTAAAGAA ATGCATTCAC AAAGCTACCC GCAAAGATGT TGCTGTGAAA 
TTTGTGAGCA AAAAAATGAA GAAGAAGGAG CAGGCTGCTC ACGAGGCCGC CCTGCTCCAG 
CACCTGCAGC ACCCTCAGTA TGTCACGCTC CATGACACCT ACGAGTCCCC CACATCCTAC 
ATCCTGATTT TGGAACTGAT GGATGACGGC CGACTCTTGG ACTACCTTAT GAATCATGAT 
GAGCTGATGG AAGAAAAGGT AGCTTTCTAC ATTCGAGACA TCATGGAAGC TCTGCAGTAC 
CTGCACAACT GTAGGGTCGC ACATTTGGAC ATAAAGCCCG AAAACCTGCT CATTGACCTC 
CGGATCCCAG TGCCTCGAGT GAAGCTCATC GACTTGGAAG ACGCTGTCCA GATCTCAGGC 
CACTTCCATA TCCACCATCT GCTGGGGAAC CCTGAGTTCG CTGCCCCCGA AGTGATCCAA 
GGCATCCCTG TCTCCCTGGG CACAGACATC TGGAGCATCG GGGTTCTGAC ATATGTCATG 
CTTAGTGGGG TCTCCCCCTT CTTAGATGAG AGCAAAGAGG AAACCTGTAT CAACGTGTGC 
AGGGTGGACT TTAGCTTCCC CCACGAGTAC TTCTGCGGTG TGAGCAATGC TGCCAGGGAT 
TTCATCAACG TAATCTTACA GGAAGACTTC CGGAGGCGGC CCACAGCAGC CACGTGCCTG 
CAGCATCCCT GGCTACAGCC CCACAACGGC AGCTACTCCA AGATCCCCTT GGACACCTCC 
CGTCTGGCCT GCTTCATAGA ACGCCGCAAG CACCAGAATG ATGTGCGGCC CATTCCCAAT 
GTCAAGAGTT ACATCGTCAA TAGGGTGAAC CAAGGGACCT CGCTATCCCA CAACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244259.1
CDS	24875..33814
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245957.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245957.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGTCGT CTTTGCTAGC AGCCCGGCAG GCTCCCCCTG ACGTGCCTAC TGCTGCAGAC 
CTTGTCAGTG CAATAGAAAA GTTGGTCAAA AATAAGCTGA CTCTGGAAGG AGGCTCTTAC 
CGGGGGAGCT TGAAGGACCC TACAGGCTGC CTGAATGAGG GGATGACCCC TCCCACACCT 
CCTAGAAACC TGGAAGAAGA ACAGAAAGCC AAGGCGCTGA GAGGCAGGAT GTTTGTCCTG 
AATGAGCTGG TACAGACAGA GAAGGACTAC GTCAAGGACC TGGGGATTGT GGTGGAGGGC 
TTCATGAAGA GAATAGAAGA GAAGGGGGTC CCTGAGGACA TGCGAGGGAA GGAAAAGATC 
GTGTTTGGAA ATATCCACCA GATTTACGAC TGGCATAAAG ATTTTTTCCT GGCTGAACTG 
GAAAAATGTA TCCAAGAGCA AGACAGATTG GCCCAGCTCT TCATTAAACA CGAGCGGAAG 
CTGCACATCT ACGTGTGGTA CTGCCAGAAC AAGCCGCGTT CCGAGTACAT CGTGGCTGAG 
TACGATGCCT ATTTTGAGGA GGTTAAACAA GAGATCAATC AAAGGCTGAC CCTTAGTGAC 
TTCCTGATCA AGCCCATTCA GAGAATAACA AAGTACCAGC TGCTACTCAA GGACTTCCTG 
CGATACAGCG AGAAGGCTGG TTTGGAGTGT TCCGATATTG AGAAAGCAGT GGAGTTAATG 
TGCCTTGTCC CCAAGCGCTG TAATGACATG ATGAATCTGG GACGCCTGCA GGGCTTCGAG 
GGCACCCTGA CCGCCCAGGG CAAGCTACTG CAGCAGGACA CATTCTATGT GATCGAGCTG 
GATGCCGGCA TGCAGTCCCG GACCAAGGAG AGGCGTGTGT TTCTCTTTGA GCAGATTGTC 
ATCTTCAGTG AACTGCTCCG AAAGGGGTCC CTCACCCCAG GCTACATGTT CAAAAGAAGC 
ATCAAGATGA ATTACTTGGT CCTGGAAGAC AATGTGGATG GTGACCCCTG CAAATTTGCA 
CTCATGAACA GGGAGACTTC AGAAAGGGTC ATTCTACAGG CTGCCAACTC AGACATCCAG 
CAGGCCTGGG TGCAGGACAT CAACCAAGTC CTAGAAACAC AGAGGGACTT CTTAAATGCA 
CTACAGTCAC CCATTGAGTA TCAGCGGAAG GAGAGAAGCA CAGCTGTGAT CCGGTCCCAG 
CCCCCGAGGG TTCCACAAGC CAGCCCCAGG CCCTACTCTT CTGGCCCGGT GGGCTCAGAG 
AAGCCCCCCA AGGGCTCCAG CTATAACCCG CCTCTGCCAC CCCTGAAGAT ATCTACCTCT 
AATGGCAGTC CAGGGTTCGA CTACCATCAG CCTGGGGACA AGTTTGACGC TAGCAAGCAG 
AACGACCTGG GAGGCTGCAA TGGGACATCA ACCATGACTG TAATCAAAGA TTACTATGCA 
CTGAAGGAGA ATGAAATCTG CGTGAGCCAA GGTGAGGTGG TACAGGTCCT CGCCGTCAAC 
CAGCAGAACA TGTGTCTGGT GTACCAGCCT GCCAGCGACC ATTCCCCGGC AGCAGAGGGC 
TGGGTCCCAG GCAGCATCCT GGCGCCCCTC GCCAAAGCCA CAGCAGCGGC CGAAAGTAGT 
GACGGGAGCA TCAAGAAGTC ATGCTCTTGG CACACTCTAC GCATGAGAAA GCGGGCAGAC 
GTGGAGAACT CGGGTAAAAA TGAAGCCACA GGGCCTCGTA AACCCAAGGA TATTCTGGGC 
AACAAAGTCT CTGTTAAAAT CTTGAATCCA AATTTCATCC AGGAAGTGGC TCCAGAATTC 
CTCGTGCCCT TAGTAGATGT GACCTGTTTG CTTGGAGACA CAGTGCTACT GCAGTGTAAA 
GCCTGTGGGC GGCCAAAGCC CAGCATCACC TGGAAGGGTC CGGATCAGAA CATCCTAGAC 
ACTGACAACA GTTCAGCAAC ATACACCATC TCCTCCTGTG ATTCTGGGGA AAGCACCCTC 
AAGATCTGCA ACCTGATGCC CCAGGATAGT GGGATTTATA CCTGCATAGC AGCAAACGAC 
CACGGGACTG CATCCACATC TGCTACAGTT AAGGTGCAAG GTGTCCCCGC AGCCCCCAAC 
CGCCCAATCG CACAGGAGAG GAGCTGCACC TCCGTCATCC TCCGCTGGCT GCCCCCAGCC 
AGCACCGGAA ACTGCACTAT TTCGGGGTAC ACAGTGGAGT ATCGCGAGGA AGGTTCCCAG 
GTCTGGCAGC AATCCGTAGC TTCGACCTTG GACACTTACC TGGTCATTGA AGACCTTAGT 
CCTGGGTGCC CTTATCAGTT CAGGGTCAGT GCCAGCAACC CCTGGGGAAT CAGCCTTCCC 
AGTGAGCCCT CGGAGTTTGT GCGCCTTCCA GAGTACGATG CAGCCGCCGA CGGTGCCACC 
ATTTCTTGGA AGGAAAATTT TGATTCTGCT TACACTGAAC TGAACGAGAT TGGCAGAGGC 
CGTTTCTCTA TTGTAAAGAA ATGCATTCAC AAAGCTACCC GCAAAGATGT TGCTGTGAAA 
TTTGTGAGCA AAAAAATGAA GAAGAAGGAG CAGGCTGCTC ACGAGGCCGC CCTGCTCCAG 
CACCTGCAGC ACCCTCAGTA TGTCACGCTC CATGACACCT ACGAGTCCCC CACATCCTAC 
ATCCTGATTT TGGAACTGAT GGATGACGGC CGACTCTTGG ACTACCTTAT GAATCATGAT 
GAGCTGATGG AAGAAAAGGT AGCTTTCTAC ATTCGAGACA TCATGGAAGC TCTGCAGTAC 
CTGCACAACT GTAGGGTCGC ACATTTGGAC ATAAAGCCCG AAAACCTGCT CATTGACCTC 
CGGATCCCAG TGCCTCGAGT GAAGCTCATC GACTTGGAAG ACGCTGTCCA GATCTCAGGC 
CACTTCCATA TCCACCATCT GCTGGGGAAC CCTGAGTTCG CTGCCCCCGA AGTGATCCAA 
GGCATCCCTG TCTCCCTGGG CACAGACATC TGGAGCATCG GGGTTCTGAC ATATGTCATG 
CTTAGTGGGG TCTCCCCCTT CTTAGATGAG AGCAAAGAGG AAACCTGTAT CAACGTGTGC 
AGGGTGGACT TTAGCTTCCC CCACGAGTAC TTCTGCGGTG TGAGCAATGC TGCCAGGGAT 
TTCATCAACG TAATCTTACA GGAAGACTTC CGGAGGCGGC CCACAGCAGC CACGTGCCTG 
CAGCATCCCT GGCTACAGCC CCACAACGGC AGCTACTCCA AGATCCCCTT GGACACCTCC 
CGTCTGGCCT GCTTCATAGA ACGCCGCAAG CACCAGAATG ATGTGCGGCC CATTCCCAAT 
GTCAAGAGTT ACATCGTCAA TAGGGTGAAC CAAGGGACCT CGCTATCCCA CAACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245958.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244260.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X11, mRNA.

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CloneID	OMu75314
Clone ID Related Accession (Same CDS sequence)	XM_011245958.2 , XM_011245959.2
Accession Version	XM_011245958.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAT TAGGATCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCTATC 
CTAAAGGAGA AAGTGGCCTT TGTGTCTGGG GGGCGTGACA AGCGAGGTGG ACCCATCCTG 
ACCTTCCCTG CCCGAAGCAA TCATGACAGA ATAAGACAGG AAGACCTACG GAAACTTGTG 
ACGTATTTGG CCAGCGTGCC AAGTGAGGAC GTTTGCAAAC GTGGCTTCAC CGTCATCATC 
GACATGCGGG GCTCCAAGTG GGATCTCATC AAGCCCCTCC TCAAGACGCT GCAGGAAGCC 
TTCCCCGCCG AGATCCATGT GGCCCTCATC ATCAAGCCTG ACAACTTCTG GCAGAAGCAG 
AAGACCAACT TTGGCAGCTC CAAATTCATC TTTGAGACAA GCATGGTGTC TGTGGAGGGC 
CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACAGAGGAGT TTGACGGCTC CCTGGATTAC 
AACCATGAGG AGTGGATTGA GTTGCGCCTC TCCCTGGAGG AGTTCTTCAA CAGTGCGGTC 
CACCTGCTCT CACGCCTTGA GGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCTGTG 
GATGTGGAGG GCTCTCGGAG GCTTATCGAC GAGCACACGC AACTTAAGAA AAAGGTGCTG 
AAGGCCCCCG TGGAAGAGCT GGACCGGGAG GGGCAGCGTC TACTGCAGTG CATCCGCTGC 
AGCGATGGCT TCTCAGGGCG CAACTGCATC CCAGGAAGTG CCGACTTCCA GAGCCTTGTA 
CCCAAGATCA CCAGCCTCCT GGACAAGCTG CATTCCACCC GACAACACCT GCACCAAATG 
TGGCACGTGC GAAAGCTCAA GCTGGATCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT 
GCTGAGAAGA TGTTCGACTG GATAAGCCAC AACAAGGAGT TATTTCTTCA GAGCCACACA 
GAGATCGGAG TCAGCTACCA GCATGCACTA GACCTCCAGA CACAGCACAA TCACTTTGCC 
ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTCGC TTCCCGCCTC 
TCAGAGGCCG GCCATTATGC CTCCCAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG 
GAGTGGAAGA GCTTTGCCGC TGCCCTGGAT GAACGCAGTA CAATCCTGGC CATGTCTGCT 
GTGTTCCACC AGAAGGCAGA GCAGTTCCTG TCAGGCGTGG ATGCCTGGTG TAAGATGTGC 
AGCGAAGGCG GCCTTCCTTC TGAGATGCAG GACCTGGAGC TGGCAATCCA CCATCACCAG 
TCCTTATATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGATGG CAAAGCACTA 
CTGGATGTCC TGCAGCGTCC CCTGAGTCCT GGGAACTCTG AGTCCCTCAC AGCCACAGCC 
AACTACTCCA AGGCGGTCCA CCAGGTGCTG GATGTGGTCC ACGAGGTGCT GCACCATCAG 
CGACGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TTCACCAGCG GCTGCAGCTG 
TGTGTCTTCC AGCAGGATGT GCAGCAGGTG CTGGACTGGA TTGAAAACCA TGGCGAGGCC 
TTTCTCAGCA AACACACTGG GGTTGGCAAG TCCTTGCATC GAGCACGTGC ACTACAGAAG 
AGGCATGATG ACTTTGAAGA AGTGGCACAG AATACATACA CCAATGCGGA CAAGCTCCTA 
GAAGCAGCGG AGCAGCTGGC TCAGACTGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA 
GCCCGGCACC TGGAGGTGCG CATCCAAGAC TTTGTGCGCA GAGTGGAGCA GCGGAAGCTG 
CTTCTGGACA TGTCGGTCTC TTTCCACACG CACACCAAAG AGCTGTGGAC ATGGATGGAG 
GACCTTCAGA AGGAGGTGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAG 
CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACCCTGG ATGCCACTCT CAATGTCATC 
AAGGAAGGCG AAGACCTTAT CCAGCAGCTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC 
ACCGAGGCCA GGGACTCAGC GATGTCCAAC AACAAGACAC CCCACAGTAG CTCTATCAGC 
CATATTGAGT CTGTCCTCCA GCAGCTGGAC GATGCTCAGG TACAGATGGA AGAGCTGTTT 
CACGAGCGGA AGATCAAGCT GGACATCTTC CTACAGCTGC GCATCTTTGA ACAGTACACC 
ATTGAGGTCA CTGCAGAGCT GGATGCCTGG AACGAGGACT TGCTCCGGCA GATGAATGAT 
TTCAACACAG AGGATCTGAC CCTGGCTGAG CAGCGGCTAC AGCGCCACAC AGAACGGAAG 
CTTGCCATGA ACAACATGAC GTTCGAGGTC ATCCAGCAGG GCCAGGACCT GCACCAGTAC 
ATCATGGAAG TGCAAGCGTC AGGAATCGAG TTAATCTGTG AGAAGGACCT TGACCTGGCA 
GCTCAGGTGC AGGAGCTGCT GGAGTTTCTC CATGAGAAGC AGCACGAGCT GGAGCTCAAT 
GCGGAGCAGA CGCACAAGCG GCTAGAGCAG TGTCTCCAGC TGAGGCACCT CCAGGCTGAG 
GTCAAACAGG TTCTGGGATG GATCCGCAAT GGAGAGTCGA TGCTCAACGC CAGCCTGGTC 
AACGCCAGCT CTTTGTCGGA GGCAGAGCAG CTGCAGCGGG AGCATGAACA GTTCCAGCTA 
GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CGCACCAGAG CGCCCTGCAG 
GTACAGCAGA AGGCAGAGGC ACTGCTCCAG GCCGGCCACT ACGACGCAGA CGCCATCCGG 
GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAACAGCTCA TGCTAAAGAT GGAGGACCGG 
CTGAAGCTTG TCAACGCCTC TGTGGCCTTT TACAAGACCT CTGAGCAGGT GTGCAGTGTT 
CTGGAGAGCT TGGAGCAGGA GTACCGGAGA GACGAGGACT GGTGTGGTGG GAGAGACAAG 
CTGGGGCCGG CGGCAGAGAT GGACCACGTT ATTCCACTCC TCAGCAAGCA CCTGGAGCAG 
AAGGAGGCCT TCCTGAAGGC CTGCACGCTG GCAAGGCGGA ACGCCGAGGT GTTCCTCAAA 
TACATCCACA GGAACAATGT GAGCATGCCT AGTGTTGCCA GCCACACCCG GGGGCCTGAA 
CAGCAAGTAA AAGCCATCTT GAGCGAGCTC TTGCAGAGGG AGAATCGAGT CCTGCACTTC 
TGGACCTTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ATGTGGTGTT TGAGCGCAGT 
GCTAAGCAGG CACTGGACTG GATCCAAGAA ACAGGAGAAT ATTACCTCTC AACACATACC 
TCCACCGGAG AGACCACGGA GGAGACTCAG GAGCTGCTGA AAGAGTATGG AGAATTCAGG 
GTGCCTGCCA AGCAAACGAA GGAGAAGGTG AAACTTCTGA TCCAGCTAGC AGACAGCTTT 
GTGGAAAAGG GCCACATCCA CGCCACAGAG ATACGGAAAT GGGTGACCAC GGTGGACAAG 
CACTACCGCG ATTTCTCCCT GAGGATGGGG AAGTACCGGT ACTCGCTGGA GAAGGCGCTG 
GGGGTCAACA CGGAGGATAA CAAGGACCTA GAGCTGGACA TCATCCCAGC AAGCCTTTCG 
GATCGGGAGG TCAAGCTCCG GGACGCTAAC CATGAGATCA ATGAAGAGAA ACGGAAGTCA 
GCTCGAAAGA AAGAGTTCAT TATGGCTGAA CTGCTCCAAA CTGAGAAGGC TTATGTAAGG 
GACTTGCACG AGTGCTTGGA GACCTACCTG TGGGAGATGA CTAGTGGTGT GGAAGAGATA 
CCCCCCGGCA TCCTCAATAA GGAACATATC ATCTTCGGCA ACATCCAGGA GATCTATGAC 
TTCCATAACA ACATCTTCCT CAAAGAGCTG GAGAAGTACG AGCAGCTGCC TGAGGATGTG 
GGACACTGCT TTGTTACCTG GGCAGACAAA TTTCAGATGT ATGTCACCTA CTGTAAAAAC 
AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCGGGCA CCTTCTTTGA TGAGATCCAG 
CAGCGGCATG GCCTGGCCAA CTCCATCTCC TCCTACCTAA TTAAGCCTGT CCAGAGGGTT 
ACCAAGTACC AGCTGCTCCT GAAGGAACTT CTAACTTGCT GTGAAGAAGG GAAAGGAGAG 
CTGAAGGATG GCCTGGAGGT GATGCTCAGT GTCCCCAAGA AAGCCAACGA TGCCATGCAT 
GTCAGCATGC TGGAAGGGTT CGATGAAAAC TTGGATGTAC AGGGGGAGTT GATTCTCCAG 
GATGCCTTTC AAGTGTGGGA CCCAAAGTCG CTGATCCGGA AGGGGCGGGA GCGCCATCTG 
TTCCTCTTTG AGATCTCCTT GGTGTTTAGC AAAGAGATCA AAGACTCTTC AGGACACACG 
AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA GCATGTAGAA 
GGTGACCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA 
GTGCTCAAAG CTTCCAACAT CGAAACCAAG CAGGAATGGA TCAAGAACAT TCGAGAAGTG 
ATTCAGGAGA GGATCATTCA TCTTAAAGGC GCTTTAAAGG AGCCAATTCA GCTCCCCAAA 
ACACCAGCCA AACTGAGGAA CAATAGTAAG AGGGATGGGG TAGAGGATGG TGACAGCCAA 
GGGGATGGGA GCAGCCAGCC AGATACCATA TCCATTGCCT CCAGGACCTC TCAGAACACA 
GTGGAAAGTG ACAAGCTCTC AGGTGGATGT GAGCTGACCG TGGTCCTCCA GGACTTCAGC 
GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGCCAGA CGGTGGAGCT GCTGGAACGA 
CCGAGTGAGA GGCCTGGCTG GTGTCTGGTC CGCACCACAG AGCGGAGCCC CCCACAGGAA 
GGCCTGGTAC CCAGCAGCGC CCTGTGCATC TCACACTCTC GCAGCAGCGT GGAAATGGAC 
TGCTTCTTCC CCTTGAAAGA TTCATACTCT CATTCCTCCA GTGAGAATGG AGGCAAATCC 
GAGTCCGTGG CCCACCTGCA GTCTCAGCCC TCCCTGAACT CCATCCACAG TTCCCCTGGT 
CCCAAGCGCT CCACCAACAC CCTCAAAAAG TGGCTGACCA GCCCCGTGCG CCGCCTCAAC 
AGCGGAAAAG CCGACGGGAA CATCAAAAAG CAGAAGAAAG TCCGGGATGG GCGGAAGAGC 
TTCGATCTGG GATCGCCCAA GCCTGGGGAT GAGACGACAC CTCAGGGAGA CAGCGCTGAC 
GAGAAGAGCA AGAAGGGTTG GGGCGAAGAC GAGCCAGATG AAGAGTCACA CACACCCCTC 
CCTCCGCCCA TGAAGATCTT TGACAACGAC CCCACACAGG ATGAGATGTC GTCTTTGCTA 
GCAGCCCGGC AGGCTCCCCC TGACGTGCCT ACTGCTGCAG ACCTTGTCAG TGCAATAGAA 
AAGTTGGTCA AAAATAAGCT GACTCTGGAA GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC 
CCTACAGGCT GCCTGAATGA GGGGATGACC CCTCCCACAC CTCCTAGAAA CCTGGAAGAA 
GAACAGAAAG CCAAGGCGCT GAGAGGCAGG ATGTTTGTCC TGAATGAGCT GGTACAGACA 
GAGAAGGACT ACGTCAAGGA CCTGGGGATT GTGGTGGAGG GCTTCATGAA GAGAATAGAA 
GAGAAGGGGG TCCCTGAGGA CATGCGAGGG AAGGAAAAGA TCGTGTTTGG AAATATCCAC 
CAGATTTACG ACTGGCATAA AGATTTTTTC CTGGCTGAAC TGGAAAAATG TATCCAAGAG 
CAAGACAGAT TGGCCCAGCT CTTCATTAAA CACGAGCGGA AGCTGCACAT CTACGTGTGG 
TACTGCCAGA ACAAGCCGCG TTCCGAGTAC ATCGTGGCTG AGTACGATGC CTATTTTGAG 
GAGGTTAAAC AAGAGATCAA TCAAAGGCTG ACCCTTAGTG ACTTCCTGAT CAAGCCCATT 
CAGAGAATAA CAAAGTACCA GCTGCTACTC AAGGACTTCC TGCGATACAG CGAGAAGGCT 
GGTTTGGAGT GTTCCGATAT TGAGAAAGCA GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC 
TGTAATGACA TGATGAATCT GGGACGCCTG CAGGGCTTCG AGGGCACCCT GACCGCCCAG 
GGCAAGCTAC TGCAGCAGGA CACATTCTAT GTGATCGAGC TGGATGCCGG CATGCAGTCC 
CGGACCAAGG AGAGGCGTGT GTTTCTCTTT GAGCAGATTG TCATCTTCAG TGAACTGCTC 
CGAAAGGGGT CCCTCACCCC AGGCTACATG TTCAAAAGAA GCATCAAGAT GAATTACTTG 
GTCCTGGAAG ACAATGTGGA TGGTGACCCC TGCAAATTTG CACTCATGAA CAGGGAGACT 
TCAGAAAGGG TCATTCTACA GGCTGCCAAC TCAGACATCC AGCAGGCCTG GGTGCAGGAC 
ATCAACCAAG TCCTAGAAAC ACAGAGGGAC TTCTTAAATG CACTACAGTC ACCCATTGAG 
TATCAGCGGA AGGAGAGAAG CACAGCTGTG ATCCGGTCCC AGCCCCCGAG GGTTCCACAA 
GCCAGCCCCA GGCCCTACTC TTCTGGCCCG GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC 
AGCTATAACC CGCCTCTGCC ACCCCTGAAG ATATCTACCT CTAATGGCAG TCCAGGGTTC 
GACTACCATC AGCCTGGGGA CAAGTTTGAC GCTAGCAAGC AGAACGACCT GGGAGGCTGC 
AATGGGACAT CAACCATGAC TGTAATCAAA GATTACTATG CACTGAAGGA GAATGAAATC 
TGCGTGAGCC AAGGTGAGGT GGTACAGGTC CTCGCCGTCA ACCAGCAGAA CATGTGTCTG 
GTGTACCAGC CTGCCAGCGA CCATTCCCCG GCAGCAGAGG GCTGGGTCCC AGGCAGCATC 
CTGGCGCCCC TCGCCAAAGC CACAGCAGCG GCCGAAAGTA GTGACGGGAG CATCAAGAAG 
TCATGCTCTT GGCACACTCT ACGCATGAGA AAGCGGGCAG ACGTGGAGAA CTCGGGTAAA 
AATGAAGCCA CAGGGCCTCG TAAACCCAAG GATATTCTGG GCAACAAAGT CTCTGTTAAA 
GAGACGAACA GTTCCGAGGA GTCAGAGTGT GATGATCTTG ACCCTAATAC TAGTATGGAG 
ATCTTGAATC CAAATTTCAT CCAGGAAGTG GCTCCAGAAT TCCTCGTGCC CTTAGTAGAT 
GTGACCTGTT TGCTTGGAGA CACAGTGCTA CTGCAGTGTA AAGCCTGTGG GCGGCCAAAG 
CCCAGCATCA CCTGGAAGGG TCCGGATCAG AACATCCTAG ACACTGACAA CAGTTCAGCA 
ACATACACCA TCTCCTCCTG TGATTCTGGG GAAAGCACCC TCAAGATCTG CAACCTGATG 
CCCCAGGATA GTGGGATTTA TACCTGCATA GCAGCAAACG ACCACGGGAC TGCATCCACA 
TCTGCTACAG TTAAGGTGCA AGGTGTCCCC GCAGCCCCCA ACCGCCCAAT CGCACAGGAG 
AGGAGCTGCA CCTCCGTCAT CCTCCGCTGG CTGCCCCCAG CCAGCACCGG AAACTGCACT 
ATTTCGGGGT ACACAGTGGA GTATCGCGAG GAAGGTTCCC AGGTCTGGCA GCAATCCGTA 
GCTTCGACCT TGGACACTTA CCTGGTCATT GAAGACCTTA GTCCTGGGTG CCCTTATCAG 
TTCAGGGTCA GTGCCAGCAA CCCCTGGGGA ATCAGCCTTC CCAGTGAGCC CTCGGAGTTT 
GTGCGCCTTC CAGAGTACGA TGCAGCCGCC GACGGTGCCA CCATTTCTTG GAAGGAAAAT 
TTTGATTCTG CTTACACTGA ACTGAACGAG ATTGGCAGAG GCCGTTTCTC TATTGTAAAG 
AAATGCATTC ACAAAGCTAC CCGCAAAGAT GTTGCTGTGA AATTTGTGAG CAAAAAAATG 
AAGAAGAAGG AGCAGGCTGC TCACGAGGCC GCCCTGCTCC AGCACCTGCA GCACCCTCAG 
TATGTCACGC TCCATGACAC CTACGAGTCC CCCACATCCT ACATCCTGAT TTTGGAACTG 
ATGGATGACG GCCGACTCTT GGACTACCTT ATGAATCATG ATGAGCTGAT GGAAGAAAAG 
GTAGCTTTCT ACATTCGAGA CATCATGGAA GCTCTGCAGT ACCTGCACAA CTGTAGGGTC 
GCACATTTGG ACATAAAGCC CGAAAACCTG CTCATTGACC TCCGGATCCC AGTGCCTCGA 
GTGAAGCTCA TCGACTTGGA AGACGCTGTC CAGATCTCAG GCCACTTCCA TATCCACCAT 
CTGCTGGGGA ACCCTGAGTT CGCTGCCCCC GAAGTGATCC AAGGCATCCC TGTCTCCCTG 
GGCACAGACA TCTGGAGCAT CGGGGTTCTG ACATATGTCA TGCTTAGTGG GGTCTCCCCC 
TTCTTAGATG AGAGCAAAGA GGAAACCTGT ATCAACGTGT GCAGGGTGGA CTTTAGCTTC 
CCCCACGAGT ACTTCTGCGG TGTGAGCAAT GCTGCCAGGG ATTTCATCAA CGTAATCTTA 
CAGGAAGACT TCCGGAGGCG GCCCACAGCA GCCACGTGCC TGCAGCATCC CTGGCTACAG 
CCCCACAACG GCAGCTACTC CAAGATCCCC TTGGACACCT CCCGTCTGGC CTGCTTCATA 
GAACGCCGCA AGCACCAGAA TGATGTGCGG CCCATTCCCA ATGTCAAGAG TTACATCGTC 
AATAGGGTGA ACCAAGGGAC CTCGCTATCC CACAACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244260.1
CDS	2051..10972
Translation	METLGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245958.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245958.2

ORF Insert Sequence:

ATGGAGACAT TAGGATCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCTATC 
CTAAAGGAGA AAGTGGCCTT TGTGTCTGGG GGGCGTGACA AGCGAGGTGG ACCCATCCTG 
ACCTTCCCTG CCCGAAGCAA TCATGACAGA ATAAGACAGG AAGACCTACG GAAACTTGTG 
ACGTATTTGG CCAGCGTGCC AAGTGAGGAC GTTTGCAAAC GTGGCTTCAC CGTCATCATC 
GACATGCGGG GCTCCAAGTG GGATCTCATC AAGCCCCTCC TCAAGACGCT GCAGGAAGCC 
TTCCCCGCCG AGATCCATGT GGCCCTCATC ATCAAGCCTG ACAACTTCTG GCAGAAGCAG 
AAGACCAACT TTGGCAGCTC CAAATTCATC TTTGAGACAA GCATGGTGTC TGTGGAGGGC 
CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACAGAGGAGT TTGACGGCTC CCTGGATTAC 
AACCATGAGG AGTGGATTGA GTTGCGCCTC TCCCTGGAGG AGTTCTTCAA CAGTGCGGTC 
CACCTGCTCT CACGCCTTGA GGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCTGTG 
GATGTGGAGG GCTCTCGGAG GCTTATCGAC GAGCACACGC AACTTAAGAA AAAGGTGCTG 
AAGGCCCCCG TGGAAGAGCT GGACCGGGAG GGGCAGCGTC TACTGCAGTG CATCCGCTGC 
AGCGATGGCT TCTCAGGGCG CAACTGCATC CCAGGAAGTG CCGACTTCCA GAGCCTTGTA 
CCCAAGATCA CCAGCCTCCT GGACAAGCTG CATTCCACCC GACAACACCT GCACCAAATG 
TGGCACGTGC GAAAGCTCAA GCTGGATCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT 
GCTGAGAAGA TGTTCGACTG GATAAGCCAC AACAAGGAGT TATTTCTTCA GAGCCACACA 
GAGATCGGAG TCAGCTACCA GCATGCACTA GACCTCCAGA CACAGCACAA TCACTTTGCC 
ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTCGC TTCCCGCCTC 
TCAGAGGCCG GCCATTATGC CTCCCAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG 
GAGTGGAAGA GCTTTGCCGC TGCCCTGGAT GAACGCAGTA CAATCCTGGC CATGTCTGCT 
GTGTTCCACC AGAAGGCAGA GCAGTTCCTG TCAGGCGTGG ATGCCTGGTG TAAGATGTGC 
AGCGAAGGCG GCCTTCCTTC TGAGATGCAG GACCTGGAGC TGGCAATCCA CCATCACCAG 
TCCTTATATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGATGG CAAAGCACTA 
CTGGATGTCC TGCAGCGTCC CCTGAGTCCT GGGAACTCTG AGTCCCTCAC AGCCACAGCC 
AACTACTCCA AGGCGGTCCA CCAGGTGCTG GATGTGGTCC ACGAGGTGCT GCACCATCAG 
CGACGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TTCACCAGCG GCTGCAGCTG 
TGTGTCTTCC AGCAGGATGT GCAGCAGGTG CTGGACTGGA TTGAAAACCA TGGCGAGGCC 
TTTCTCAGCA AACACACTGG GGTTGGCAAG TCCTTGCATC GAGCACGTGC ACTACAGAAG 
AGGCATGATG ACTTTGAAGA AGTGGCACAG AATACATACA CCAATGCGGA CAAGCTCCTA 
GAAGCAGCGG AGCAGCTGGC TCAGACTGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA 
GCCCGGCACC TGGAGGTGCG CATCCAAGAC TTTGTGCGCA GAGTGGAGCA GCGGAAGCTG 
CTTCTGGACA TGTCGGTCTC TTTCCACACG CACACCAAAG AGCTGTGGAC ATGGATGGAG 
GACCTTCAGA AGGAGGTGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAG 
CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACCCTGG ATGCCACTCT CAATGTCATC 
AAGGAAGGCG AAGACCTTAT CCAGCAGCTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC 
ACCGAGGCCA GGGACTCAGC GATGTCCAAC AACAAGACAC CCCACAGTAG CTCTATCAGC 
CATATTGAGT CTGTCCTCCA GCAGCTGGAC GATGCTCAGG TACAGATGGA AGAGCTGTTT 
CACGAGCGGA AGATCAAGCT GGACATCTTC CTACAGCTGC GCATCTTTGA ACAGTACACC 
ATTGAGGTCA CTGCAGAGCT GGATGCCTGG AACGAGGACT TGCTCCGGCA GATGAATGAT 
TTCAACACAG AGGATCTGAC CCTGGCTGAG CAGCGGCTAC AGCGCCACAC AGAACGGAAG 
CTTGCCATGA ACAACATGAC GTTCGAGGTC ATCCAGCAGG GCCAGGACCT GCACCAGTAC 
ATCATGGAAG TGCAAGCGTC AGGAATCGAG TTAATCTGTG AGAAGGACCT TGACCTGGCA 
GCTCAGGTGC AGGAGCTGCT GGAGTTTCTC CATGAGAAGC AGCACGAGCT GGAGCTCAAT 
GCGGAGCAGA CGCACAAGCG GCTAGAGCAG TGTCTCCAGC TGAGGCACCT CCAGGCTGAG 
GTCAAACAGG TTCTGGGATG GATCCGCAAT GGAGAGTCGA TGCTCAACGC CAGCCTGGTC 
AACGCCAGCT CTTTGTCGGA GGCAGAGCAG CTGCAGCGGG AGCATGAACA GTTCCAGCTA 
GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CGCACCAGAG CGCCCTGCAG 
GTACAGCAGA AGGCAGAGGC ACTGCTCCAG GCCGGCCACT ACGACGCAGA CGCCATCCGG 
GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAACAGCTCA TGCTAAAGAT GGAGGACCGG 
CTGAAGCTTG TCAACGCCTC TGTGGCCTTT TACAAGACCT CTGAGCAGGT GTGCAGTGTT 
CTGGAGAGCT TGGAGCAGGA GTACCGGAGA GACGAGGACT GGTGTGGTGG GAGAGACAAG 
CTGGGGCCGG CGGCAGAGAT GGACCACGTT ATTCCACTCC TCAGCAAGCA CCTGGAGCAG 
AAGGAGGCCT TCCTGAAGGC CTGCACGCTG GCAAGGCGGA ACGCCGAGGT GTTCCTCAAA 
TACATCCACA GGAACAATGT GAGCATGCCT AGTGTTGCCA GCCACACCCG GGGGCCTGAA 
CAGCAAGTAA AAGCCATCTT GAGCGAGCTC TTGCAGAGGG AGAATCGAGT CCTGCACTTC 
TGGACCTTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ATGTGGTGTT TGAGCGCAGT 
GCTAAGCAGG CACTGGACTG GATCCAAGAA ACAGGAGAAT ATTACCTCTC AACACATACC 
TCCACCGGAG AGACCACGGA GGAGACTCAG GAGCTGCTGA AAGAGTATGG AGAATTCAGG 
GTGCCTGCCA AGCAAACGAA GGAGAAGGTG AAACTTCTGA TCCAGCTAGC AGACAGCTTT 
GTGGAAAAGG GCCACATCCA CGCCACAGAG ATACGGAAAT GGGTGACCAC GGTGGACAAG 
CACTACCGCG ATTTCTCCCT GAGGATGGGG AAGTACCGGT ACTCGCTGGA GAAGGCGCTG 
GGGGTCAACA CGGAGGATAA CAAGGACCTA GAGCTGGACA TCATCCCAGC AAGCCTTTCG 
GATCGGGAGG TCAAGCTCCG GGACGCTAAC CATGAGATCA ATGAAGAGAA ACGGAAGTCA 
GCTCGAAAGA AAGAGTTCAT TATGGCTGAA CTGCTCCAAA CTGAGAAGGC TTATGTAAGG 
GACTTGCACG AGTGCTTGGA GACCTACCTG TGGGAGATGA CTAGTGGTGT GGAAGAGATA 
CCCCCCGGCA TCCTCAATAA GGAACATATC ATCTTCGGCA ACATCCAGGA GATCTATGAC 
TTCCATAACA ACATCTTCCT CAAAGAGCTG GAGAAGTACG AGCAGCTGCC TGAGGATGTG 
GGACACTGCT TTGTTACCTG GGCAGACAAA TTTCAGATGT ATGTCACCTA CTGTAAAAAC 
AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCGGGCA CCTTCTTTGA TGAGATCCAG 
CAGCGGCATG GCCTGGCCAA CTCCATCTCC TCCTACCTAA TTAAGCCTGT CCAGAGGGTT 
ACCAAGTACC AGCTGCTCCT GAAGGAACTT CTAACTTGCT GTGAAGAAGG GAAAGGAGAG 
CTGAAGGATG GCCTGGAGGT GATGCTCAGT GTCCCCAAGA AAGCCAACGA TGCCATGCAT 
GTCAGCATGC TGGAAGGGTT CGATGAAAAC TTGGATGTAC AGGGGGAGTT GATTCTCCAG 
GATGCCTTTC AAGTGTGGGA CCCAAAGTCG CTGATCCGGA AGGGGCGGGA GCGCCATCTG 
TTCCTCTTTG AGATCTCCTT GGTGTTTAGC AAAGAGATCA AAGACTCTTC AGGACACACG 
AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA GCATGTAGAA 
GGTGACCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA 
GTGCTCAAAG CTTCCAACAT CGAAACCAAG CAGGAATGGA TCAAGAACAT TCGAGAAGTG 
ATTCAGGAGA GGATCATTCA TCTTAAAGGC GCTTTAAAGG AGCCAATTCA GCTCCCCAAA 
ACACCAGCCA AACTGAGGAA CAATAGTAAG AGGGATGGGG TAGAGGATGG TGACAGCCAA 
GGGGATGGGA GCAGCCAGCC AGATACCATA TCCATTGCCT CCAGGACCTC TCAGAACACA 
GTGGAAAGTG ACAAGCTCTC AGGTGGATGT GAGCTGACCG TGGTCCTCCA GGACTTCAGC 
GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGCCAGA CGGTGGAGCT GCTGGAACGA 
CCGAGTGAGA GGCCTGGCTG GTGTCTGGTC CGCACCACAG AGCGGAGCCC CCCACAGGAA 
GGCCTGGTAC CCAGCAGCGC CCTGTGCATC TCACACTCTC GCAGCAGCGT GGAAATGGAC 
TGCTTCTTCC CCTTGAAAGA TTCATACTCT CATTCCTCCA GTGAGAATGG AGGCAAATCC 
GAGTCCGTGG CCCACCTGCA GTCTCAGCCC TCCCTGAACT CCATCCACAG TTCCCCTGGT 
CCCAAGCGCT CCACCAACAC CCTCAAAAAG TGGCTGACCA GCCCCGTGCG CCGCCTCAAC 
AGCGGAAAAG CCGACGGGAA CATCAAAAAG CAGAAGAAAG TCCGGGATGG GCGGAAGAGC 
TTCGATCTGG GATCGCCCAA GCCTGGGGAT GAGACGACAC CTCAGGGAGA CAGCGCTGAC 
GAGAAGAGCA AGAAGGGTTG GGGCGAAGAC GAGCCAGATG AAGAGTCACA CACACCCCTC 
CCTCCGCCCA TGAAGATCTT TGACAACGAC CCCACACAGG ATGAGATGTC GTCTTTGCTA 
GCAGCCCGGC AGGCTCCCCC TGACGTGCCT ACTGCTGCAG ACCTTGTCAG TGCAATAGAA 
AAGTTGGTCA AAAATAAGCT GACTCTGGAA GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC 
CCTACAGGCT GCCTGAATGA GGGGATGACC CCTCCCACAC CTCCTAGAAA CCTGGAAGAA 
GAACAGAAAG CCAAGGCGCT GAGAGGCAGG ATGTTTGTCC TGAATGAGCT GGTACAGACA 
GAGAAGGACT ACGTCAAGGA CCTGGGGATT GTGGTGGAGG GCTTCATGAA GAGAATAGAA 
GAGAAGGGGG TCCCTGAGGA CATGCGAGGG AAGGAAAAGA TCGTGTTTGG AAATATCCAC 
CAGATTTACG ACTGGCATAA AGATTTTTTC CTGGCTGAAC TGGAAAAATG TATCCAAGAG 
CAAGACAGAT TGGCCCAGCT CTTCATTAAA CACGAGCGGA AGCTGCACAT CTACGTGTGG 
TACTGCCAGA ACAAGCCGCG TTCCGAGTAC ATCGTGGCTG AGTACGATGC CTATTTTGAG 
GAGGTTAAAC AAGAGATCAA TCAAAGGCTG ACCCTTAGTG ACTTCCTGAT CAAGCCCATT 
CAGAGAATAA CAAAGTACCA GCTGCTACTC AAGGACTTCC TGCGATACAG CGAGAAGGCT 
GGTTTGGAGT GTTCCGATAT TGAGAAAGCA GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC 
TGTAATGACA TGATGAATCT GGGACGCCTG CAGGGCTTCG AGGGCACCCT GACCGCCCAG 
GGCAAGCTAC TGCAGCAGGA CACATTCTAT GTGATCGAGC TGGATGCCGG CATGCAGTCC 
CGGACCAAGG AGAGGCGTGT GTTTCTCTTT GAGCAGATTG TCATCTTCAG TGAACTGCTC 
CGAAAGGGGT CCCTCACCCC AGGCTACATG TTCAAAAGAA GCATCAAGAT GAATTACTTG 
GTCCTGGAAG ACAATGTGGA TGGTGACCCC TGCAAATTTG CACTCATGAA CAGGGAGACT 
TCAGAAAGGG TCATTCTACA GGCTGCCAAC TCAGACATCC AGCAGGCCTG GGTGCAGGAC 
ATCAACCAAG TCCTAGAAAC ACAGAGGGAC TTCTTAAATG CACTACAGTC ACCCATTGAG 
TATCAGCGGA AGGAGAGAAG CACAGCTGTG ATCCGGTCCC AGCCCCCGAG GGTTCCACAA 
GCCAGCCCCA GGCCCTACTC TTCTGGCCCG GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC 
AGCTATAACC CGCCTCTGCC ACCCCTGAAG ATATCTACCT CTAATGGCAG TCCAGGGTTC 
GACTACCATC AGCCTGGGGA CAAGTTTGAC GCTAGCAAGC AGAACGACCT GGGAGGCTGC 
AATGGGACAT CAACCATGAC TGTAATCAAA GATTACTATG CACTGAAGGA GAATGAAATC 
TGCGTGAGCC AAGGTGAGGT GGTACAGGTC CTCGCCGTCA ACCAGCAGAA CATGTGTCTG 
GTGTACCAGC CTGCCAGCGA CCATTCCCCG GCAGCAGAGG GCTGGGTCCC AGGCAGCATC 
CTGGCGCCCC TCGCCAAAGC CACAGCAGCG GCCGAAAGTA GTGACGGGAG CATCAAGAAG 
TCATGCTCTT GGCACACTCT ACGCATGAGA AAGCGGGCAG ACGTGGAGAA CTCGGGTAAA 
AATGAAGCCA CAGGGCCTCG TAAACCCAAG GATATTCTGG GCAACAAAGT CTCTGTTAAA 
GAGACGAACA GTTCCGAGGA GTCAGAGTGT GATGATCTTG ACCCTAATAC TAGTATGGAG 
ATCTTGAATC CAAATTTCAT CCAGGAAGTG GCTCCAGAAT TCCTCGTGCC CTTAGTAGAT 
GTGACCTGTT TGCTTGGAGA CACAGTGCTA CTGCAGTGTA AAGCCTGTGG GCGGCCAAAG 
CCCAGCATCA CCTGGAAGGG TCCGGATCAG AACATCCTAG ACACTGACAA CAGTTCAGCA 
ACATACACCA TCTCCTCCTG TGATTCTGGG GAAAGCACCC TCAAGATCTG CAACCTGATG 
CCCCAGGATA GTGGGATTTA TACCTGCATA GCAGCAAACG ACCACGGGAC TGCATCCACA 
TCTGCTACAG TTAAGGTGCA AGGTGTCCCC GCAGCCCCCA ACCGCCCAAT CGCACAGGAG 
AGGAGCTGCA CCTCCGTCAT CCTCCGCTGG CTGCCCCCAG CCAGCACCGG AAACTGCACT 
ATTTCGGGGT ACACAGTGGA GTATCGCGAG GAAGGTTCCC AGGTCTGGCA GCAATCCGTA 
GCTTCGACCT TGGACACTTA CCTGGTCATT GAAGACCTTA GTCCTGGGTG CCCTTATCAG 
TTCAGGGTCA GTGCCAGCAA CCCCTGGGGA ATCAGCCTTC CCAGTGAGCC CTCGGAGTTT 
GTGCGCCTTC CAGAGTACGA TGCAGCCGCC GACGGTGCCA CCATTTCTTG GAAGGAAAAT 
TTTGATTCTG CTTACACTGA ACTGAACGAG ATTGGCAGAG GCCGTTTCTC TATTGTAAAG 
AAATGCATTC ACAAAGCTAC CCGCAAAGAT GTTGCTGTGA AATTTGTGAG CAAAAAAATG 
AAGAAGAAGG AGCAGGCTGC TCACGAGGCC GCCCTGCTCC AGCACCTGCA GCACCCTCAG 
TATGTCACGC TCCATGACAC CTACGAGTCC CCCACATCCT ACATCCTGAT TTTGGAACTG 
ATGGATGACG GCCGACTCTT GGACTACCTT ATGAATCATG ATGAGCTGAT GGAAGAAAAG 
GTAGCTTTCT ACATTCGAGA CATCATGGAA GCTCTGCAGT ACCTGCACAA CTGTAGGGTC 
GCACATTTGG ACATAAAGCC CGAAAACCTG CTCATTGACC TCCGGATCCC AGTGCCTCGA 
GTGAAGCTCA TCGACTTGGA AGACGCTGTC CAGATCTCAG GCCACTTCCA TATCCACCAT 
CTGCTGGGGA ACCCTGAGTT CGCTGCCCCC GAAGTGATCC AAGGCATCCC TGTCTCCCTG 
GGCACAGACA TCTGGAGCAT CGGGGTTCTG ACATATGTCA TGCTTAGTGG GGTCTCCCCC 
TTCTTAGATG AGAGCAAAGA GGAAACCTGT ATCAACGTGT GCAGGGTGGA CTTTAGCTTC 
CCCCACGAGT ACTTCTGCGG TGTGAGCAAT GCTGCCAGGG ATTTCATCAA CGTAATCTTA 
CAGGAAGACT TCCGGAGGCG GCCCACAGCA GCCACGTGCC TGCAGCATCC CTGGCTACAG 
CCCCACAACG GCAGCTACTC CAAGATCCCC TTGGACACCT CCCGTCTGGC CTGCTTCATA 
GAACGCCGCA AGCACCAGAA TGATGTGCGG CCCATTCCCA ATGTCAAGAG TTACATCGTC 
AATAGGGTGA ACCAAGGGAC CTCGCTATCC CACAACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245959.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244261.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X12, mRNA.

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CloneID	OMu75314
Clone ID Related Accession (Same CDS sequence)	XM_011245958.2 , XM_011245959.2
Accession Version	XM_011245959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245959.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAT TAGGATCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCTATC 
CTAAAGGAGA AAGTGGCCTT TGTGTCTGGG GGGCGTGACA AGCGAGGTGG ACCCATCCTG 
ACCTTCCCTG CCCGAAGCAA TCATGACAGA ATAAGACAGG AAGACCTACG GAAACTTGTG 
ACGTATTTGG CCAGCGTGCC AAGTGAGGAC GTTTGCAAAC GTGGCTTCAC CGTCATCATC 
GACATGCGGG GCTCCAAGTG GGATCTCATC AAGCCCCTCC TCAAGACGCT GCAGGAAGCC 
TTCCCCGCCG AGATCCATGT GGCCCTCATC ATCAAGCCTG ACAACTTCTG GCAGAAGCAG 
AAGACCAACT TTGGCAGCTC CAAATTCATC TTTGAGACAA GCATGGTGTC TGTGGAGGGC 
CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACAGAGGAGT TTGACGGCTC CCTGGATTAC 
AACCATGAGG AGTGGATTGA GTTGCGCCTC TCCCTGGAGG AGTTCTTCAA CAGTGCGGTC 
CACCTGCTCT CACGCCTTGA GGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCTGTG 
GATGTGGAGG GCTCTCGGAG GCTTATCGAC GAGCACACGC AACTTAAGAA AAAGGTGCTG 
AAGGCCCCCG TGGAAGAGCT GGACCGGGAG GGGCAGCGTC TACTGCAGTG CATCCGCTGC 
AGCGATGGCT TCTCAGGGCG CAACTGCATC CCAGGAAGTG CCGACTTCCA GAGCCTTGTA 
CCCAAGATCA CCAGCCTCCT GGACAAGCTG CATTCCACCC GACAACACCT GCACCAAATG 
TGGCACGTGC GAAAGCTCAA GCTGGATCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT 
GCTGAGAAGA TGTTCGACTG GATAAGCCAC AACAAGGAGT TATTTCTTCA GAGCCACACA 
GAGATCGGAG TCAGCTACCA GCATGCACTA GACCTCCAGA CACAGCACAA TCACTTTGCC 
ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTCGC TTCCCGCCTC 
TCAGAGGCCG GCCATTATGC CTCCCAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG 
GAGTGGAAGA GCTTTGCCGC TGCCCTGGAT GAACGCAGTA CAATCCTGGC CATGTCTGCT 
GTGTTCCACC AGAAGGCAGA GCAGTTCCTG TCAGGCGTGG ATGCCTGGTG TAAGATGTGC 
AGCGAAGGCG GCCTTCCTTC TGAGATGCAG GACCTGGAGC TGGCAATCCA CCATCACCAG 
TCCTTATATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGATGG CAAAGCACTA 
CTGGATGTCC TGCAGCGTCC CCTGAGTCCT GGGAACTCTG AGTCCCTCAC AGCCACAGCC 
AACTACTCCA AGGCGGTCCA CCAGGTGCTG GATGTGGTCC ACGAGGTGCT GCACCATCAG 
CGACGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TTCACCAGCG GCTGCAGCTG 
TGTGTCTTCC AGCAGGATGT GCAGCAGGTG CTGGACTGGA TTGAAAACCA TGGCGAGGCC 
TTTCTCAGCA AACACACTGG GGTTGGCAAG TCCTTGCATC GAGCACGTGC ACTACAGAAG 
AGGCATGATG ACTTTGAAGA AGTGGCACAG AATACATACA CCAATGCGGA CAAGCTCCTA 
GAAGCAGCGG AGCAGCTGGC TCAGACTGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA 
GCCCGGCACC TGGAGGTGCG CATCCAAGAC TTTGTGCGCA GAGTGGAGCA GCGGAAGCTG 
CTTCTGGACA TGTCGGTCTC TTTCCACACG CACACCAAAG AGCTGTGGAC ATGGATGGAG 
GACCTTCAGA AGGAGGTGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAG 
CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACCCTGG ATGCCACTCT CAATGTCATC 
AAGGAAGGCG AAGACCTTAT CCAGCAGCTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC 
ACCGAGGCCA GGGACTCAGC GATGTCCAAC AACAAGACAC CCCACAGTAG CTCTATCAGC 
CATATTGAGT CTGTCCTCCA GCAGCTGGAC GATGCTCAGG TACAGATGGA AGAGCTGTTT 
CACGAGCGGA AGATCAAGCT GGACATCTTC CTACAGCTGC GCATCTTTGA ACAGTACACC 
ATTGAGGTCA CTGCAGAGCT GGATGCCTGG AACGAGGACT TGCTCCGGCA GATGAATGAT 
TTCAACACAG AGGATCTGAC CCTGGCTGAG CAGCGGCTAC AGCGCCACAC AGAACGGAAG 
CTTGCCATGA ACAACATGAC GTTCGAGGTC ATCCAGCAGG GCCAGGACCT GCACCAGTAC 
ATCATGGAAG TGCAAGCGTC AGGAATCGAG TTAATCTGTG AGAAGGACCT TGACCTGGCA 
GCTCAGGTGC AGGAGCTGCT GGAGTTTCTC CATGAGAAGC AGCACGAGCT GGAGCTCAAT 
GCGGAGCAGA CGCACAAGCG GCTAGAGCAG TGTCTCCAGC TGAGGCACCT CCAGGCTGAG 
GTCAAACAGG TTCTGGGATG GATCCGCAAT GGAGAGTCGA TGCTCAACGC CAGCCTGGTC 
AACGCCAGCT CTTTGTCGGA GGCAGAGCAG CTGCAGCGGG AGCATGAACA GTTCCAGCTA 
GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CGCACCAGAG CGCCCTGCAG 
GTACAGCAGA AGGCAGAGGC ACTGCTCCAG GCCGGCCACT ACGACGCAGA CGCCATCCGG 
GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAACAGCTCA TGCTAAAGAT GGAGGACCGG 
CTGAAGCTTG TCAACGCCTC TGTGGCCTTT TACAAGACCT CTGAGCAGGT GTGCAGTGTT 
CTGGAGAGCT TGGAGCAGGA GTACCGGAGA GACGAGGACT GGTGTGGTGG GAGAGACAAG 
CTGGGGCCGG CGGCAGAGAT GGACCACGTT ATTCCACTCC TCAGCAAGCA CCTGGAGCAG 
AAGGAGGCCT TCCTGAAGGC CTGCACGCTG GCAAGGCGGA ACGCCGAGGT GTTCCTCAAA 
TACATCCACA GGAACAATGT GAGCATGCCT AGTGTTGCCA GCCACACCCG GGGGCCTGAA 
CAGCAAGTAA AAGCCATCTT GAGCGAGCTC TTGCAGAGGG AGAATCGAGT CCTGCACTTC 
TGGACCTTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ATGTGGTGTT TGAGCGCAGT 
GCTAAGCAGG CACTGGACTG GATCCAAGAA ACAGGAGAAT ATTACCTCTC AACACATACC 
TCCACCGGAG AGACCACGGA GGAGACTCAG GAGCTGCTGA AAGAGTATGG AGAATTCAGG 
GTGCCTGCCA AGCAAACGAA GGAGAAGGTG AAACTTCTGA TCCAGCTAGC AGACAGCTTT 
GTGGAAAAGG GCCACATCCA CGCCACAGAG ATACGGAAAT GGGTGACCAC GGTGGACAAG 
CACTACCGCG ATTTCTCCCT GAGGATGGGG AAGTACCGGT ACTCGCTGGA GAAGGCGCTG 
GGGGTCAACA CGGAGGATAA CAAGGACCTA GAGCTGGACA TCATCCCAGC AAGCCTTTCG 
GATCGGGAGG TCAAGCTCCG GGACGCTAAC CATGAGATCA ATGAAGAGAA ACGGAAGTCA 
GCTCGAAAGA AAGAGTTCAT TATGGCTGAA CTGCTCCAAA CTGAGAAGGC TTATGTAAGG 
GACTTGCACG AGTGCTTGGA GACCTACCTG TGGGAGATGA CTAGTGGTGT GGAAGAGATA 
CCCCCCGGCA TCCTCAATAA GGAACATATC ATCTTCGGCA ACATCCAGGA GATCTATGAC 
TTCCATAACA ACATCTTCCT CAAAGAGCTG GAGAAGTACG AGCAGCTGCC TGAGGATGTG 
GGACACTGCT TTGTTACCTG GGCAGACAAA TTTCAGATGT ATGTCACCTA CTGTAAAAAC 
AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCGGGCA CCTTCTTTGA TGAGATCCAG 
CAGCGGCATG GCCTGGCCAA CTCCATCTCC TCCTACCTAA TTAAGCCTGT CCAGAGGGTT 
ACCAAGTACC AGCTGCTCCT GAAGGAACTT CTAACTTGCT GTGAAGAAGG GAAAGGAGAG 
CTGAAGGATG GCCTGGAGGT GATGCTCAGT GTCCCCAAGA AAGCCAACGA TGCCATGCAT 
GTCAGCATGC TGGAAGGGTT CGATGAAAAC TTGGATGTAC AGGGGGAGTT GATTCTCCAG 
GATGCCTTTC AAGTGTGGGA CCCAAAGTCG CTGATCCGGA AGGGGCGGGA GCGCCATCTG 
TTCCTCTTTG AGATCTCCTT GGTGTTTAGC AAAGAGATCA AAGACTCTTC AGGACACACG 
AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA GCATGTAGAA 
GGTGACCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA 
GTGCTCAAAG CTTCCAACAT CGAAACCAAG CAGGAATGGA TCAAGAACAT TCGAGAAGTG 
ATTCAGGAGA GGATCATTCA TCTTAAAGGC GCTTTAAAGG AGCCAATTCA GCTCCCCAAA 
ACACCAGCCA AACTGAGGAA CAATAGTAAG AGGGATGGGG TAGAGGATGG TGACAGCCAA 
GGGGATGGGA GCAGCCAGCC AGATACCATA TCCATTGCCT CCAGGACCTC TCAGAACACA 
GTGGAAAGTG ACAAGCTCTC AGGTGGATGT GAGCTGACCG TGGTCCTCCA GGACTTCAGC 
GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGCCAGA CGGTGGAGCT GCTGGAACGA 
CCGAGTGAGA GGCCTGGCTG GTGTCTGGTC CGCACCACAG AGCGGAGCCC CCCACAGGAA 
GGCCTGGTAC CCAGCAGCGC CCTGTGCATC TCACACTCTC GCAGCAGCGT GGAAATGGAC 
TGCTTCTTCC CCTTGAAAGA TTCATACTCT CATTCCTCCA GTGAGAATGG AGGCAAATCC 
GAGTCCGTGG CCCACCTGCA GTCTCAGCCC TCCCTGAACT CCATCCACAG TTCCCCTGGT 
CCCAAGCGCT CCACCAACAC CCTCAAAAAG TGGCTGACCA GCCCCGTGCG CCGCCTCAAC 
AGCGGAAAAG CCGACGGGAA CATCAAAAAG CAGAAGAAAG TCCGGGATGG GCGGAAGAGC 
TTCGATCTGG GATCGCCCAA GCCTGGGGAT GAGACGACAC CTCAGGGAGA CAGCGCTGAC 
GAGAAGAGCA AGAAGGGTTG GGGCGAAGAC GAGCCAGATG AAGAGTCACA CACACCCCTC 
CCTCCGCCCA TGAAGATCTT TGACAACGAC CCCACACAGG ATGAGATGTC GTCTTTGCTA 
GCAGCCCGGC AGGCTCCCCC TGACGTGCCT ACTGCTGCAG ACCTTGTCAG TGCAATAGAA 
AAGTTGGTCA AAAATAAGCT GACTCTGGAA GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC 
CCTACAGGCT GCCTGAATGA GGGGATGACC CCTCCCACAC CTCCTAGAAA CCTGGAAGAA 
GAACAGAAAG CCAAGGCGCT GAGAGGCAGG ATGTTTGTCC TGAATGAGCT GGTACAGACA 
GAGAAGGACT ACGTCAAGGA CCTGGGGATT GTGGTGGAGG GCTTCATGAA GAGAATAGAA 
GAGAAGGGGG TCCCTGAGGA CATGCGAGGG AAGGAAAAGA TCGTGTTTGG AAATATCCAC 
CAGATTTACG ACTGGCATAA AGATTTTTTC CTGGCTGAAC TGGAAAAATG TATCCAAGAG 
CAAGACAGAT TGGCCCAGCT CTTCATTAAA CACGAGCGGA AGCTGCACAT CTACGTGTGG 
TACTGCCAGA ACAAGCCGCG TTCCGAGTAC ATCGTGGCTG AGTACGATGC CTATTTTGAG 
GAGGTTAAAC AAGAGATCAA TCAAAGGCTG ACCCTTAGTG ACTTCCTGAT CAAGCCCATT 
CAGAGAATAA CAAAGTACCA GCTGCTACTC AAGGACTTCC TGCGATACAG CGAGAAGGCT 
GGTTTGGAGT GTTCCGATAT TGAGAAAGCA GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC 
TGTAATGACA TGATGAATCT GGGACGCCTG CAGGGCTTCG AGGGCACCCT GACCGCCCAG 
GGCAAGCTAC TGCAGCAGGA CACATTCTAT GTGATCGAGC TGGATGCCGG CATGCAGTCC 
CGGACCAAGG AGAGGCGTGT GTTTCTCTTT GAGCAGATTG TCATCTTCAG TGAACTGCTC 
CGAAAGGGGT CCCTCACCCC AGGCTACATG TTCAAAAGAA GCATCAAGAT GAATTACTTG 
GTCCTGGAAG ACAATGTGGA TGGTGACCCC TGCAAATTTG CACTCATGAA CAGGGAGACT 
TCAGAAAGGG TCATTCTACA GGCTGCCAAC TCAGACATCC AGCAGGCCTG GGTGCAGGAC 
ATCAACCAAG TCCTAGAAAC ACAGAGGGAC TTCTTAAATG CACTACAGTC ACCCATTGAG 
TATCAGCGGA AGGAGAGAAG CACAGCTGTG ATCCGGTCCC AGCCCCCGAG GGTTCCACAA 
GCCAGCCCCA GGCCCTACTC TTCTGGCCCG GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC 
AGCTATAACC CGCCTCTGCC ACCCCTGAAG ATATCTACCT CTAATGGCAG TCCAGGGTTC 
GACTACCATC AGCCTGGGGA CAAGTTTGAC GCTAGCAAGC AGAACGACCT GGGAGGCTGC 
AATGGGACAT CAACCATGAC TGTAATCAAA GATTACTATG CACTGAAGGA GAATGAAATC 
TGCGTGAGCC AAGGTGAGGT GGTACAGGTC CTCGCCGTCA ACCAGCAGAA CATGTGTCTG 
GTGTACCAGC CTGCCAGCGA CCATTCCCCG GCAGCAGAGG GCTGGGTCCC AGGCAGCATC 
CTGGCGCCCC TCGCCAAAGC CACAGCAGCG GCCGAAAGTA GTGACGGGAG CATCAAGAAG 
TCATGCTCTT GGCACACTCT ACGCATGAGA AAGCGGGCAG ACGTGGAGAA CTCGGGTAAA 
AATGAAGCCA CAGGGCCTCG TAAACCCAAG GATATTCTGG GCAACAAAGT CTCTGTTAAA 
GAGACGAACA GTTCCGAGGA GTCAGAGTGT GATGATCTTG ACCCTAATAC TAGTATGGAG 
ATCTTGAATC CAAATTTCAT CCAGGAAGTG GCTCCAGAAT TCCTCGTGCC CTTAGTAGAT 
GTGACCTGTT TGCTTGGAGA CACAGTGCTA CTGCAGTGTA AAGCCTGTGG GCGGCCAAAG 
CCCAGCATCA CCTGGAAGGG TCCGGATCAG AACATCCTAG ACACTGACAA CAGTTCAGCA 
ACATACACCA TCTCCTCCTG TGATTCTGGG GAAAGCACCC TCAAGATCTG CAACCTGATG 
CCCCAGGATA GTGGGATTTA TACCTGCATA GCAGCAAACG ACCACGGGAC TGCATCCACA 
TCTGCTACAG TTAAGGTGCA AGGTGTCCCC GCAGCCCCCA ACCGCCCAAT CGCACAGGAG 
AGGAGCTGCA CCTCCGTCAT CCTCCGCTGG CTGCCCCCAG CCAGCACCGG AAACTGCACT 
ATTTCGGGGT ACACAGTGGA GTATCGCGAG GAAGGTTCCC AGGTCTGGCA GCAATCCGTA 
GCTTCGACCT TGGACACTTA CCTGGTCATT GAAGACCTTA GTCCTGGGTG CCCTTATCAG 
TTCAGGGTCA GTGCCAGCAA CCCCTGGGGA ATCAGCCTTC CCAGTGAGCC CTCGGAGTTT 
GTGCGCCTTC CAGAGTACGA TGCAGCCGCC GACGGTGCCA CCATTTCTTG GAAGGAAAAT 
TTTGATTCTG CTTACACTGA ACTGAACGAG ATTGGCAGAG GCCGTTTCTC TATTGTAAAG 
AAATGCATTC ACAAAGCTAC CCGCAAAGAT GTTGCTGTGA AATTTGTGAG CAAAAAAATG 
AAGAAGAAGG AGCAGGCTGC TCACGAGGCC GCCCTGCTCC AGCACCTGCA GCACCCTCAG 
TATGTCACGC TCCATGACAC CTACGAGTCC CCCACATCCT ACATCCTGAT TTTGGAACTG 
ATGGATGACG GCCGACTCTT GGACTACCTT ATGAATCATG ATGAGCTGAT GGAAGAAAAG 
GTAGCTTTCT ACATTCGAGA CATCATGGAA GCTCTGCAGT ACCTGCACAA CTGTAGGGTC 
GCACATTTGG ACATAAAGCC CGAAAACCTG CTCATTGACC TCCGGATCCC AGTGCCTCGA 
GTGAAGCTCA TCGACTTGGA AGACGCTGTC CAGATCTCAG GCCACTTCCA TATCCACCAT 
CTGCTGGGGA ACCCTGAGTT CGCTGCCCCC GAAGTGATCC AAGGCATCCC TGTCTCCCTG 
GGCACAGACA TCTGGAGCAT CGGGGTTCTG ACATATGTCA TGCTTAGTGG GGTCTCCCCC 
TTCTTAGATG AGAGCAAAGA GGAAACCTGT ATCAACGTGT GCAGGGTGGA CTTTAGCTTC 
CCCCACGAGT ACTTCTGCGG TGTGAGCAAT GCTGCCAGGG ATTTCATCAA CGTAATCTTA 
CAGGAAGACT TCCGGAGGCG GCCCACAGCA GCCACGTGCC TGCAGCATCC CTGGCTACAG 
CCCCACAACG GCAGCTACTC CAAGATCCCC TTGGACACCT CCCGTCTGGC CTGCTTCATA 
GAACGCCGCA AGCACCAGAA TGATGTGCGG CCCATTCCCA ATGTCAAGAG TTACATCGTC 
AATAGGGTGA ACCAAGGGAC CTCGCTATCC CACAACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244261.1
CDS	1420..10341
Translation	METLGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245959.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245959.2

ORF Insert Sequence:

ATGGAGACAT TAGGATCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCTATC 
CTAAAGGAGA AAGTGGCCTT TGTGTCTGGG GGGCGTGACA AGCGAGGTGG ACCCATCCTG 
ACCTTCCCTG CCCGAAGCAA TCATGACAGA ATAAGACAGG AAGACCTACG GAAACTTGTG 
ACGTATTTGG CCAGCGTGCC AAGTGAGGAC GTTTGCAAAC GTGGCTTCAC CGTCATCATC 
GACATGCGGG GCTCCAAGTG GGATCTCATC AAGCCCCTCC TCAAGACGCT GCAGGAAGCC 
TTCCCCGCCG AGATCCATGT GGCCCTCATC ATCAAGCCTG ACAACTTCTG GCAGAAGCAG 
AAGACCAACT TTGGCAGCTC CAAATTCATC TTTGAGACAA GCATGGTGTC TGTGGAGGGC 
CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACAGAGGAGT TTGACGGCTC CCTGGATTAC 
AACCATGAGG AGTGGATTGA GTTGCGCCTC TCCCTGGAGG AGTTCTTCAA CAGTGCGGTC 
CACCTGCTCT CACGCCTTGA GGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCTGTG 
GATGTGGAGG GCTCTCGGAG GCTTATCGAC GAGCACACGC AACTTAAGAA AAAGGTGCTG 
AAGGCCCCCG TGGAAGAGCT GGACCGGGAG GGGCAGCGTC TACTGCAGTG CATCCGCTGC 
AGCGATGGCT TCTCAGGGCG CAACTGCATC CCAGGAAGTG CCGACTTCCA GAGCCTTGTA 
CCCAAGATCA CCAGCCTCCT GGACAAGCTG CATTCCACCC GACAACACCT GCACCAAATG 
TGGCACGTGC GAAAGCTCAA GCTGGATCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT 
GCTGAGAAGA TGTTCGACTG GATAAGCCAC AACAAGGAGT TATTTCTTCA GAGCCACACA 
GAGATCGGAG TCAGCTACCA GCATGCACTA GACCTCCAGA CACAGCACAA TCACTTTGCC 
ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTCGC TTCCCGCCTC 
TCAGAGGCCG GCCATTATGC CTCCCAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG 
GAGTGGAAGA GCTTTGCCGC TGCCCTGGAT GAACGCAGTA CAATCCTGGC CATGTCTGCT 
GTGTTCCACC AGAAGGCAGA GCAGTTCCTG TCAGGCGTGG ATGCCTGGTG TAAGATGTGC 
AGCGAAGGCG GCCTTCCTTC TGAGATGCAG GACCTGGAGC TGGCAATCCA CCATCACCAG 
TCCTTATATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGATGG CAAAGCACTA 
CTGGATGTCC TGCAGCGTCC CCTGAGTCCT GGGAACTCTG AGTCCCTCAC AGCCACAGCC 
AACTACTCCA AGGCGGTCCA CCAGGTGCTG GATGTGGTCC ACGAGGTGCT GCACCATCAG 
CGACGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TTCACCAGCG GCTGCAGCTG 
TGTGTCTTCC AGCAGGATGT GCAGCAGGTG CTGGACTGGA TTGAAAACCA TGGCGAGGCC 
TTTCTCAGCA AACACACTGG GGTTGGCAAG TCCTTGCATC GAGCACGTGC ACTACAGAAG 
AGGCATGATG ACTTTGAAGA AGTGGCACAG AATACATACA CCAATGCGGA CAAGCTCCTA 
GAAGCAGCGG AGCAGCTGGC TCAGACTGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA 
GCCCGGCACC TGGAGGTGCG CATCCAAGAC TTTGTGCGCA GAGTGGAGCA GCGGAAGCTG 
CTTCTGGACA TGTCGGTCTC TTTCCACACG CACACCAAAG AGCTGTGGAC ATGGATGGAG 
GACCTTCAGA AGGAGGTGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAG 
CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACCCTGG ATGCCACTCT CAATGTCATC 
AAGGAAGGCG AAGACCTTAT CCAGCAGCTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC 
ACCGAGGCCA GGGACTCAGC GATGTCCAAC AACAAGACAC CCCACAGTAG CTCTATCAGC 
CATATTGAGT CTGTCCTCCA GCAGCTGGAC GATGCTCAGG TACAGATGGA AGAGCTGTTT 
CACGAGCGGA AGATCAAGCT GGACATCTTC CTACAGCTGC GCATCTTTGA ACAGTACACC 
ATTGAGGTCA CTGCAGAGCT GGATGCCTGG AACGAGGACT TGCTCCGGCA GATGAATGAT 
TTCAACACAG AGGATCTGAC CCTGGCTGAG CAGCGGCTAC AGCGCCACAC AGAACGGAAG 
CTTGCCATGA ACAACATGAC GTTCGAGGTC ATCCAGCAGG GCCAGGACCT GCACCAGTAC 
ATCATGGAAG TGCAAGCGTC AGGAATCGAG TTAATCTGTG AGAAGGACCT TGACCTGGCA 
GCTCAGGTGC AGGAGCTGCT GGAGTTTCTC CATGAGAAGC AGCACGAGCT GGAGCTCAAT 
GCGGAGCAGA CGCACAAGCG GCTAGAGCAG TGTCTCCAGC TGAGGCACCT CCAGGCTGAG 
GTCAAACAGG TTCTGGGATG GATCCGCAAT GGAGAGTCGA TGCTCAACGC CAGCCTGGTC 
AACGCCAGCT CTTTGTCGGA GGCAGAGCAG CTGCAGCGGG AGCATGAACA GTTCCAGCTA 
GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CGCACCAGAG CGCCCTGCAG 
GTACAGCAGA AGGCAGAGGC ACTGCTCCAG GCCGGCCACT ACGACGCAGA CGCCATCCGG 
GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAACAGCTCA TGCTAAAGAT GGAGGACCGG 
CTGAAGCTTG TCAACGCCTC TGTGGCCTTT TACAAGACCT CTGAGCAGGT GTGCAGTGTT 
CTGGAGAGCT TGGAGCAGGA GTACCGGAGA GACGAGGACT GGTGTGGTGG GAGAGACAAG 
CTGGGGCCGG CGGCAGAGAT GGACCACGTT ATTCCACTCC TCAGCAAGCA CCTGGAGCAG 
AAGGAGGCCT TCCTGAAGGC CTGCACGCTG GCAAGGCGGA ACGCCGAGGT GTTCCTCAAA 
TACATCCACA GGAACAATGT GAGCATGCCT AGTGTTGCCA GCCACACCCG GGGGCCTGAA 
CAGCAAGTAA AAGCCATCTT GAGCGAGCTC TTGCAGAGGG AGAATCGAGT CCTGCACTTC 
TGGACCTTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ATGTGGTGTT TGAGCGCAGT 
GCTAAGCAGG CACTGGACTG GATCCAAGAA ACAGGAGAAT ATTACCTCTC AACACATACC 
TCCACCGGAG AGACCACGGA GGAGACTCAG GAGCTGCTGA AAGAGTATGG AGAATTCAGG 
GTGCCTGCCA AGCAAACGAA GGAGAAGGTG AAACTTCTGA TCCAGCTAGC AGACAGCTTT 
GTGGAAAAGG GCCACATCCA CGCCACAGAG ATACGGAAAT GGGTGACCAC GGTGGACAAG 
CACTACCGCG ATTTCTCCCT GAGGATGGGG AAGTACCGGT ACTCGCTGGA GAAGGCGCTG 
GGGGTCAACA CGGAGGATAA CAAGGACCTA GAGCTGGACA TCATCCCAGC AAGCCTTTCG 
GATCGGGAGG TCAAGCTCCG GGACGCTAAC CATGAGATCA ATGAAGAGAA ACGGAAGTCA 
GCTCGAAAGA AAGAGTTCAT TATGGCTGAA CTGCTCCAAA CTGAGAAGGC TTATGTAAGG 
GACTTGCACG AGTGCTTGGA GACCTACCTG TGGGAGATGA CTAGTGGTGT GGAAGAGATA 
CCCCCCGGCA TCCTCAATAA GGAACATATC ATCTTCGGCA ACATCCAGGA GATCTATGAC 
TTCCATAACA ACATCTTCCT CAAAGAGCTG GAGAAGTACG AGCAGCTGCC TGAGGATGTG 
GGACACTGCT TTGTTACCTG GGCAGACAAA TTTCAGATGT ATGTCACCTA CTGTAAAAAC 
AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCGGGCA CCTTCTTTGA TGAGATCCAG 
CAGCGGCATG GCCTGGCCAA CTCCATCTCC TCCTACCTAA TTAAGCCTGT CCAGAGGGTT 
ACCAAGTACC AGCTGCTCCT GAAGGAACTT CTAACTTGCT GTGAAGAAGG GAAAGGAGAG 
CTGAAGGATG GCCTGGAGGT GATGCTCAGT GTCCCCAAGA AAGCCAACGA TGCCATGCAT 
GTCAGCATGC TGGAAGGGTT CGATGAAAAC TTGGATGTAC AGGGGGAGTT GATTCTCCAG 
GATGCCTTTC AAGTGTGGGA CCCAAAGTCG CTGATCCGGA AGGGGCGGGA GCGCCATCTG 
TTCCTCTTTG AGATCTCCTT GGTGTTTAGC AAAGAGATCA AAGACTCTTC AGGACACACG 
AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA GCATGTAGAA 
GGTGACCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA 
GTGCTCAAAG CTTCCAACAT CGAAACCAAG CAGGAATGGA TCAAGAACAT TCGAGAAGTG 
ATTCAGGAGA GGATCATTCA TCTTAAAGGC GCTTTAAAGG AGCCAATTCA GCTCCCCAAA 
ACACCAGCCA AACTGAGGAA CAATAGTAAG AGGGATGGGG TAGAGGATGG TGACAGCCAA 
GGGGATGGGA GCAGCCAGCC AGATACCATA TCCATTGCCT CCAGGACCTC TCAGAACACA 
GTGGAAAGTG ACAAGCTCTC AGGTGGATGT GAGCTGACCG TGGTCCTCCA GGACTTCAGC 
GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGCCAGA CGGTGGAGCT GCTGGAACGA 
CCGAGTGAGA GGCCTGGCTG GTGTCTGGTC CGCACCACAG AGCGGAGCCC CCCACAGGAA 
GGCCTGGTAC CCAGCAGCGC CCTGTGCATC TCACACTCTC GCAGCAGCGT GGAAATGGAC 
TGCTTCTTCC CCTTGAAAGA TTCATACTCT CATTCCTCCA GTGAGAATGG AGGCAAATCC 
GAGTCCGTGG CCCACCTGCA GTCTCAGCCC TCCCTGAACT CCATCCACAG TTCCCCTGGT 
CCCAAGCGCT CCACCAACAC CCTCAAAAAG TGGCTGACCA GCCCCGTGCG CCGCCTCAAC 
AGCGGAAAAG CCGACGGGAA CATCAAAAAG CAGAAGAAAG TCCGGGATGG GCGGAAGAGC 
TTCGATCTGG GATCGCCCAA GCCTGGGGAT GAGACGACAC CTCAGGGAGA CAGCGCTGAC 
GAGAAGAGCA AGAAGGGTTG GGGCGAAGAC GAGCCAGATG AAGAGTCACA CACACCCCTC 
CCTCCGCCCA TGAAGATCTT TGACAACGAC CCCACACAGG ATGAGATGTC GTCTTTGCTA 
GCAGCCCGGC AGGCTCCCCC TGACGTGCCT ACTGCTGCAG ACCTTGTCAG TGCAATAGAA 
AAGTTGGTCA AAAATAAGCT GACTCTGGAA GGAGGCTCTT ACCGGGGGAG CTTGAAGGAC 
CCTACAGGCT GCCTGAATGA GGGGATGACC CCTCCCACAC CTCCTAGAAA CCTGGAAGAA 
GAACAGAAAG CCAAGGCGCT GAGAGGCAGG ATGTTTGTCC TGAATGAGCT GGTACAGACA 
GAGAAGGACT ACGTCAAGGA CCTGGGGATT GTGGTGGAGG GCTTCATGAA GAGAATAGAA 
GAGAAGGGGG TCCCTGAGGA CATGCGAGGG AAGGAAAAGA TCGTGTTTGG AAATATCCAC 
CAGATTTACG ACTGGCATAA AGATTTTTTC CTGGCTGAAC TGGAAAAATG TATCCAAGAG 
CAAGACAGAT TGGCCCAGCT CTTCATTAAA CACGAGCGGA AGCTGCACAT CTACGTGTGG 
TACTGCCAGA ACAAGCCGCG TTCCGAGTAC ATCGTGGCTG AGTACGATGC CTATTTTGAG 
GAGGTTAAAC AAGAGATCAA TCAAAGGCTG ACCCTTAGTG ACTTCCTGAT CAAGCCCATT 
CAGAGAATAA CAAAGTACCA GCTGCTACTC AAGGACTTCC TGCGATACAG CGAGAAGGCT 
GGTTTGGAGT GTTCCGATAT TGAGAAAGCA GTGGAGTTAA TGTGCCTTGT CCCCAAGCGC 
TGTAATGACA TGATGAATCT GGGACGCCTG CAGGGCTTCG AGGGCACCCT GACCGCCCAG 
GGCAAGCTAC TGCAGCAGGA CACATTCTAT GTGATCGAGC TGGATGCCGG CATGCAGTCC 
CGGACCAAGG AGAGGCGTGT GTTTCTCTTT GAGCAGATTG TCATCTTCAG TGAACTGCTC 
CGAAAGGGGT CCCTCACCCC AGGCTACATG TTCAAAAGAA GCATCAAGAT GAATTACTTG 
GTCCTGGAAG ACAATGTGGA TGGTGACCCC TGCAAATTTG CACTCATGAA CAGGGAGACT 
TCAGAAAGGG TCATTCTACA GGCTGCCAAC TCAGACATCC AGCAGGCCTG GGTGCAGGAC 
ATCAACCAAG TCCTAGAAAC ACAGAGGGAC TTCTTAAATG CACTACAGTC ACCCATTGAG 
TATCAGCGGA AGGAGAGAAG CACAGCTGTG ATCCGGTCCC AGCCCCCGAG GGTTCCACAA 
GCCAGCCCCA GGCCCTACTC TTCTGGCCCG GTGGGCTCAG AGAAGCCCCC CAAGGGCTCC 
AGCTATAACC CGCCTCTGCC ACCCCTGAAG ATATCTACCT CTAATGGCAG TCCAGGGTTC 
GACTACCATC AGCCTGGGGA CAAGTTTGAC GCTAGCAAGC AGAACGACCT GGGAGGCTGC 
AATGGGACAT CAACCATGAC TGTAATCAAA GATTACTATG CACTGAAGGA GAATGAAATC 
TGCGTGAGCC AAGGTGAGGT GGTACAGGTC CTCGCCGTCA ACCAGCAGAA CATGTGTCTG 
GTGTACCAGC CTGCCAGCGA CCATTCCCCG GCAGCAGAGG GCTGGGTCCC AGGCAGCATC 
CTGGCGCCCC TCGCCAAAGC CACAGCAGCG GCCGAAAGTA GTGACGGGAG CATCAAGAAG 
TCATGCTCTT GGCACACTCT ACGCATGAGA AAGCGGGCAG ACGTGGAGAA CTCGGGTAAA 
AATGAAGCCA CAGGGCCTCG TAAACCCAAG GATATTCTGG GCAACAAAGT CTCTGTTAAA 
GAGACGAACA GTTCCGAGGA GTCAGAGTGT GATGATCTTG ACCCTAATAC TAGTATGGAG 
ATCTTGAATC CAAATTTCAT CCAGGAAGTG GCTCCAGAAT TCCTCGTGCC CTTAGTAGAT 
GTGACCTGTT TGCTTGGAGA CACAGTGCTA CTGCAGTGTA AAGCCTGTGG GCGGCCAAAG 
CCCAGCATCA CCTGGAAGGG TCCGGATCAG AACATCCTAG ACACTGACAA CAGTTCAGCA 
ACATACACCA TCTCCTCCTG TGATTCTGGG GAAAGCACCC TCAAGATCTG CAACCTGATG 
CCCCAGGATA GTGGGATTTA TACCTGCATA GCAGCAAACG ACCACGGGAC TGCATCCACA 
TCTGCTACAG TTAAGGTGCA AGGTGTCCCC GCAGCCCCCA ACCGCCCAAT CGCACAGGAG 
AGGAGCTGCA CCTCCGTCAT CCTCCGCTGG CTGCCCCCAG CCAGCACCGG AAACTGCACT 
ATTTCGGGGT ACACAGTGGA GTATCGCGAG GAAGGTTCCC AGGTCTGGCA GCAATCCGTA 
GCTTCGACCT TGGACACTTA CCTGGTCATT GAAGACCTTA GTCCTGGGTG CCCTTATCAG 
TTCAGGGTCA GTGCCAGCAA CCCCTGGGGA ATCAGCCTTC CCAGTGAGCC CTCGGAGTTT 
GTGCGCCTTC CAGAGTACGA TGCAGCCGCC GACGGTGCCA CCATTTCTTG GAAGGAAAAT 
TTTGATTCTG CTTACACTGA ACTGAACGAG ATTGGCAGAG GCCGTTTCTC TATTGTAAAG 
AAATGCATTC ACAAAGCTAC CCGCAAAGAT GTTGCTGTGA AATTTGTGAG CAAAAAAATG 
AAGAAGAAGG AGCAGGCTGC TCACGAGGCC GCCCTGCTCC AGCACCTGCA GCACCCTCAG 
TATGTCACGC TCCATGACAC CTACGAGTCC CCCACATCCT ACATCCTGAT TTTGGAACTG 
ATGGATGACG GCCGACTCTT GGACTACCTT ATGAATCATG ATGAGCTGAT GGAAGAAAAG 
GTAGCTTTCT ACATTCGAGA CATCATGGAA GCTCTGCAGT ACCTGCACAA CTGTAGGGTC 
GCACATTTGG ACATAAAGCC CGAAAACCTG CTCATTGACC TCCGGATCCC AGTGCCTCGA 
GTGAAGCTCA TCGACTTGGA AGACGCTGTC CAGATCTCAG GCCACTTCCA TATCCACCAT 
CTGCTGGGGA ACCCTGAGTT CGCTGCCCCC GAAGTGATCC AAGGCATCCC TGTCTCCCTG 
GGCACAGACA TCTGGAGCAT CGGGGTTCTG ACATATGTCA TGCTTAGTGG GGTCTCCCCC 
TTCTTAGATG AGAGCAAAGA GGAAACCTGT ATCAACGTGT GCAGGGTGGA CTTTAGCTTC 
CCCCACGAGT ACTTCTGCGG TGTGAGCAAT GCTGCCAGGG ATTTCATCAA CGTAATCTTA 
CAGGAAGACT TCCGGAGGCG GCCCACAGCA GCCACGTGCC TGCAGCATCC CTGGCTACAG 
CCCCACAACG GCAGCTACTC CAAGATCCCC TTGGACACCT CCCGTCTGGC CTGCTTCATA 
GAACGCCGCA AGCACCAGAA TGATGTGCGG CCCATTCCCA ATGTCAAGAG TTACATCGTC 
AATAGGGTGA ACCAAGGGAC CTCGCTATCC CACAACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245960.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244262.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X13, mRNA.

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CloneID	OMu75315
Clone ID Related Accession (Same CDS sequence)	XM_011245960.2 , XM_011245960.3
Accession Version	XM_011245960.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245960.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGACTC TGGAAGGAGG CTCTTACCGG GGGAGCTTGA AGGACCCTAC AGGCTGCCTG 
AATGAGGGGA TGACCCCTCC CACACCTCCT AGAAACCTGG AAGAAGAACA GAAAGCCAAG 
GCGCTGAGAG GCAGGATGTT TGTCCTGAAT GAGCTGGTAC AGACAGAGAA GGACTACGTC 
AAGGACCTGG GGATTGTGGT GGAGGGCTTC ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT 
GAGGACATGC GAGGGAAGGA AAAGATCGTG TTTGGAAATA TCCACCAGAT TTACGACTGG 
CATAAAGATT TTTTCCTGGC TGAACTGGAA AAATGTATCC AAGAGCAAGA CAGATTGGCC 
CAGCTCTTCA TTAAACACGA GCGGAAGCTG CACATCTACG TGTGGTACTG CCAGAACAAG 
CCGCGTTCCG AGTACATCGT GGCTGAGTAC GATGCCTATT TTGAGGAGGT TAAACAAGAG 
ATCAATCAAA GGCTGACCCT TAGTGACTTC CTGATCAAGC CCATTCAGAG AATAACAAAG 
TACCAGCTGC TACTCAAGGA CTTCCTGCGA TACAGCGAGA AGGCTGGTTT GGAGTGTTCC 
GATATTGAGA AAGCAGTGGA GTTAATGTGC CTTGTCCCCA AGCGCTGTAA TGACATGATG 
AATCTGGGAC GCCTGCAGGG CTTCGAGGGC ACCCTGACCG CCCAGGGCAA GCTACTGCAG 
CAGGACACAT TCTATGTGAT CGAGCTGGAT GCCGGCATGC AGTCCCGGAC CAAGGAGAGG 
CGTGTGTTTC TCTTTGAGCA GATTGTCATC TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC 
ACCCCAGGCT ACATGTTCAA AAGAAGCATC AAGATGAATT ACTTGGTCCT GGAAGACAAT 
GTGGATGGTG ACCCCTGCAA ATTTGCACTC ATGAACAGGG AGACTTCAGA AAGGGTCATT 
CTACAGGCTG CCAACTCAGA CATCCAGCAG GCCTGGGTGC AGGACATCAA CCAAGTCCTA 
GAAACACAGA GGGACTTCTT AAATGCACTA CAGTCACCCA TTGAGTATCA GCGGAAGGAG 
AGAAGCACAG CTGTGATCCG GTCCCAGCCC CCGAGGGTTC CACAAGCCAG CCCCAGGCCC 
TACTCTTCTG GCCCGGTGGG CTCAGAGAAG CCCCCCAAGG GCTCCAGCTA TAACCCGCCT 
CTGCCACCCC TGAAGATATC TACCTCTAAT GGCAGTCCAG GGTTCGACTA CCATCAGCCT 
GGGGACAAGT TTGACGCTAG CAAGCAGAAC GACCTGGGAG GCTGCAATGG GACATCAACC 
ATGACTGTAA TCAAAGATTA CTATGCACTG AAGGAGAATG AAATCTGCGT GAGCCAAGGT 
GAGGTGGTAC AGGTCCTCGC CGTCAACCAG CAGAACATGT GTCTGGTGTA CCAGCCTGCC 
AGCGACCATT CCCCGGCAGC AGAGGGCTGG GTCCCAGGCA GCATCCTGGC GCCCCTCGCC 
AAAGCCACAG CAGCGGCCGA AAGTAGTGAC GGGAGCATCA AGAAGTCATG CTCTTGGCAC 
ACTCTACGCA TGAGAAAGCG GGCAGACGTG GAGAACTCGG GTAAAAATGA AGCCACAGGG 
CCTCGTAAAC CCAAGGATAT TCTGGGCAAC AAAGTCTCTG TTAAAGAGAC GAACAGTTCC 
GAGGAGTCAG AGTGTGATGA TCTTGACCCT AATACTAGTA TGGAGATCTT GAATCCAAAT 
TTCATCCAGG AAGTGGCTCC AGAATTCCTC GTGCCCTTAG TAGATGTGAC CTGTTTGCTT 
GGAGACACAG TGCTACTGCA GTGTAAAGCC TGTGGGCGGC CAAAGCCCAG CATCACCTGG 
AAGGGTCCGG ATCAGAACAT CCTAGACACT GACAACAGTT CAGCAACATA CACCATCTCC 
TCCTGTGATT CTGGGGAAAG CACCCTCAAG ATCTGCAACC TGATGCCCCA GGATAGTGGG 
ATTTATACCT GCATAGCAGC AAACGACCAC GGGACTGCAT CCACATCTGC TACAGTTAAG 
GTGCAAGGTG TCCCCGCAGC CCCCAACCGC CCAATCGCAC AGGAGAGGAG CTGCACCTCC 
GTCATCCTCC GCTGGCTGCC CCCAGCCAGC ACCGGAAACT GCACTATTTC GGGGTACACA 
GTGGAGTATC GCGAGGAAGG TTCCCAGGTC TGGCAGCAAT CCGTAGCTTC GACCTTGGAC 
ACTTACCTGG TCATTGAAGA CCTTAGTCCT GGGTGCCCTT ATCAGTTCAG GGTCAGTGCC 
AGCAACCCCT GGGGAATCAG CCTTCCCAGT GAGCCCTCGG AGTTTGTGCG CCTTCCAGAG 
TACGATGCAG CCGCCGACGG TGCCACCATT TCTTGGAAGG AAAATTTTGA TTCTGCTTAC 
ACTGAACTGA ACGAGATTGG CAGAGGCCGT TTCTCTATTG TAAAGAAATG CATTCACAAA 
GCTACCCGCA AAGATGTTGC TGTGAAATTT GTGAGCAAAA AAATGAAGAA GAAGGAGCAG 
GCTGCTCACG AGGCCGCCCT GCTCCAGCAC CTGCAGCACC CTCAGTATGT CACGCTCCAT 
GACACCTACG AGTCCCCCAC ATCCTACATC CTGATTTTGG AACTGATGGA TGACGGCCGA 
CTCTTGGACT ACCTTATGAA TCATGATGAG CTGATGGAAG AAAAGGTAGC TTTCTACATT 
CGAGACATCA TGGAAGCTCT GCAGTACCTG CACAACTGTA GGGTCGCACA TTTGGACATA 
AAGCCCGAAA ACCTGCTCAT TGACCTCCGG ATCCCAGTGC CTCGAGTGAA GCTCATCGAC 
TTGGAAGACG CTGTCCAGAT CTCAGGCCAC TTCCATATCC ACCATCTGCT GGGGAACCCT 
GAGTTCGCTG CCCCCGAAGT GATCCAAGGC ATCCCTGTCT CCCTGGGCAC AGACATCTGG 
AGCATCGGGG TTCTGACATA TGTCATGCTT AGTGGGGTCT CCCCCTTCTT AGATGAGAGC 
AAAGAGGAAA CCTGTATCAA CGTGTGCAGG GTGGACTTTA GCTTCCCCCA CGAGTACTTC 
TGCGGTGTGA GCAATGCTGC CAGGGATTTC ATCAACGTAA TCTTACAGGA AGACTTCCGG 
AGGCGGCCCA CAGCAGCCAC GTGCCTGCAG CATCCCTGGC TACAGCCCCA CAACGGCAGC 
TACTCCAAGA TCCCCTTGGA CACCTCCCGT CTGGCCTGCT TCATAGAACG CCGCAAGCAC 
CAGAATGATG TGCGGCCCAT TCCCAATGTC AAGAGTTACA TCGTCAATAG GGTGAACCAA 
GGGACCTCGC TATCCCACAA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244262.1
CDS	4891..13797
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245960.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245960.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGACTC TGGAAGGAGG CTCTTACCGG GGGAGCTTGA AGGACCCTAC AGGCTGCCTG 
AATGAGGGGA TGACCCCTCC CACACCTCCT AGAAACCTGG AAGAAGAACA GAAAGCCAAG 
GCGCTGAGAG GCAGGATGTT TGTCCTGAAT GAGCTGGTAC AGACAGAGAA GGACTACGTC 
AAGGACCTGG GGATTGTGGT GGAGGGCTTC ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT 
GAGGACATGC GAGGGAAGGA AAAGATCGTG TTTGGAAATA TCCACCAGAT TTACGACTGG 
CATAAAGATT TTTTCCTGGC TGAACTGGAA AAATGTATCC AAGAGCAAGA CAGATTGGCC 
CAGCTCTTCA TTAAACACGA GCGGAAGCTG CACATCTACG TGTGGTACTG CCAGAACAAG 
CCGCGTTCCG AGTACATCGT GGCTGAGTAC GATGCCTATT TTGAGGAGGT TAAACAAGAG 
ATCAATCAAA GGCTGACCCT TAGTGACTTC CTGATCAAGC CCATTCAGAG AATAACAAAG 
TACCAGCTGC TACTCAAGGA CTTCCTGCGA TACAGCGAGA AGGCTGGTTT GGAGTGTTCC 
GATATTGAGA AAGCAGTGGA GTTAATGTGC CTTGTCCCCA AGCGCTGTAA TGACATGATG 
AATCTGGGAC GCCTGCAGGG CTTCGAGGGC ACCCTGACCG CCCAGGGCAA GCTACTGCAG 
CAGGACACAT TCTATGTGAT CGAGCTGGAT GCCGGCATGC AGTCCCGGAC CAAGGAGAGG 
CGTGTGTTTC TCTTTGAGCA GATTGTCATC TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC 
ACCCCAGGCT ACATGTTCAA AAGAAGCATC AAGATGAATT ACTTGGTCCT GGAAGACAAT 
GTGGATGGTG ACCCCTGCAA ATTTGCACTC ATGAACAGGG AGACTTCAGA AAGGGTCATT 
CTACAGGCTG CCAACTCAGA CATCCAGCAG GCCTGGGTGC AGGACATCAA CCAAGTCCTA 
GAAACACAGA GGGACTTCTT AAATGCACTA CAGTCACCCA TTGAGTATCA GCGGAAGGAG 
AGAAGCACAG CTGTGATCCG GTCCCAGCCC CCGAGGGTTC CACAAGCCAG CCCCAGGCCC 
TACTCTTCTG GCCCGGTGGG CTCAGAGAAG CCCCCCAAGG GCTCCAGCTA TAACCCGCCT 
CTGCCACCCC TGAAGATATC TACCTCTAAT GGCAGTCCAG GGTTCGACTA CCATCAGCCT 
GGGGACAAGT TTGACGCTAG CAAGCAGAAC GACCTGGGAG GCTGCAATGG GACATCAACC 
ATGACTGTAA TCAAAGATTA CTATGCACTG AAGGAGAATG AAATCTGCGT GAGCCAAGGT 
GAGGTGGTAC AGGTCCTCGC CGTCAACCAG CAGAACATGT GTCTGGTGTA CCAGCCTGCC 
AGCGACCATT CCCCGGCAGC AGAGGGCTGG GTCCCAGGCA GCATCCTGGC GCCCCTCGCC 
AAAGCCACAG CAGCGGCCGA AAGTAGTGAC GGGAGCATCA AGAAGTCATG CTCTTGGCAC 
ACTCTACGCA TGAGAAAGCG GGCAGACGTG GAGAACTCGG GTAAAAATGA AGCCACAGGG 
CCTCGTAAAC CCAAGGATAT TCTGGGCAAC AAAGTCTCTG TTAAAGAGAC GAACAGTTCC 
GAGGAGTCAG AGTGTGATGA TCTTGACCCT AATACTAGTA TGGAGATCTT GAATCCAAAT 
TTCATCCAGG AAGTGGCTCC AGAATTCCTC GTGCCCTTAG TAGATGTGAC CTGTTTGCTT 
GGAGACACAG TGCTACTGCA GTGTAAAGCC TGTGGGCGGC CAAAGCCCAG CATCACCTGG 
AAGGGTCCGG ATCAGAACAT CCTAGACACT GACAACAGTT CAGCAACATA CACCATCTCC 
TCCTGTGATT CTGGGGAAAG CACCCTCAAG ATCTGCAACC TGATGCCCCA GGATAGTGGG 
ATTTATACCT GCATAGCAGC AAACGACCAC GGGACTGCAT CCACATCTGC TACAGTTAAG 
GTGCAAGGTG TCCCCGCAGC CCCCAACCGC CCAATCGCAC AGGAGAGGAG CTGCACCTCC 
GTCATCCTCC GCTGGCTGCC CCCAGCCAGC ACCGGAAACT GCACTATTTC GGGGTACACA 
GTGGAGTATC GCGAGGAAGG TTCCCAGGTC TGGCAGCAAT CCGTAGCTTC GACCTTGGAC 
ACTTACCTGG TCATTGAAGA CCTTAGTCCT GGGTGCCCTT ATCAGTTCAG GGTCAGTGCC 
AGCAACCCCT GGGGAATCAG CCTTCCCAGT GAGCCCTCGG AGTTTGTGCG CCTTCCAGAG 
TACGATGCAG CCGCCGACGG TGCCACCATT TCTTGGAAGG AAAATTTTGA TTCTGCTTAC 
ACTGAACTGA ACGAGATTGG CAGAGGCCGT TTCTCTATTG TAAAGAAATG CATTCACAAA 
GCTACCCGCA AAGATGTTGC TGTGAAATTT GTGAGCAAAA AAATGAAGAA GAAGGAGCAG 
GCTGCTCACG AGGCCGCCCT GCTCCAGCAC CTGCAGCACC CTCAGTATGT CACGCTCCAT 
GACACCTACG AGTCCCCCAC ATCCTACATC CTGATTTTGG AACTGATGGA TGACGGCCGA 
CTCTTGGACT ACCTTATGAA TCATGATGAG CTGATGGAAG AAAAGGTAGC TTTCTACATT 
CGAGACATCA TGGAAGCTCT GCAGTACCTG CACAACTGTA GGGTCGCACA TTTGGACATA 
AAGCCCGAAA ACCTGCTCAT TGACCTCCGG ATCCCAGTGC CTCGAGTGAA GCTCATCGAC 
TTGGAAGACG CTGTCCAGAT CTCAGGCCAC TTCCATATCC ACCATCTGCT GGGGAACCCT 
GAGTTCGCTG CCCCCGAAGT GATCCAAGGC ATCCCTGTCT CCCTGGGCAC AGACATCTGG 
AGCATCGGGG TTCTGACATA TGTCATGCTT AGTGGGGTCT CCCCCTTCTT AGATGAGAGC 
AAAGAGGAAA CCTGTATCAA CGTGTGCAGG GTGGACTTTA GCTTCCCCCA CGAGTACTTC 
TGCGGTGTGA GCAATGCTGC CAGGGATTTC ATCAACGTAA TCTTACAGGA AGACTTCCGG 
AGGCGGCCCA CAGCAGCCAC GTGCCTGCAG CATCCCTGGC TACAGCCCCA CAACGGCAGC 
TACTCCAAGA TCCCCTTGGA CACCTCCCGT CTGGCCTGCT TCATAGAACG CCGCAAGCAC 
CAGAATGATG TGCGGCCCAT TCCCAATGTC AAGAGTTACA TCGTCAATAG GGTGAACCAA 
GGGACCTCGC TATCCCACAA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245960.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244262.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X13, mRNA.

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CloneID	OMu75315
Clone ID Related Accession (Same CDS sequence)	XM_011245960.2 , XM_011245960.3
Accession Version	XM_011245960.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245960.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGACTC TGGAAGGAGG CTCTTACCGG GGGAGCTTGA AGGACCCTAC AGGCTGCCTG 
AATGAGGGGA TGACCCCTCC CACACCTCCT AGAAACCTGG AAGAAGAACA GAAAGCCAAG 
GCGCTGAGAG GCAGGATGTT TGTCCTGAAT GAGCTGGTAC AGACAGAGAA GGACTACGTC 
AAGGACCTGG GGATTGTGGT GGAGGGCTTC ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT 
GAGGACATGC GAGGGAAGGA AAAGATCGTG TTTGGAAATA TCCACCAGAT TTACGACTGG 
CATAAAGATT TTTTCCTGGC TGAACTGGAA AAATGTATCC AAGAGCAAGA CAGATTGGCC 
CAGCTCTTCA TTAAACACGA GCGGAAGCTG CACATCTACG TGTGGTACTG CCAGAACAAG 
CCGCGTTCCG AGTACATCGT GGCTGAGTAC GATGCCTATT TTGAGGAGGT TAAACAAGAG 
ATCAATCAAA GGCTGACCCT TAGTGACTTC CTGATCAAGC CCATTCAGAG AATAACAAAG 
TACCAGCTGC TACTCAAGGA CTTCCTGCGA TACAGCGAGA AGGCTGGTTT GGAGTGTTCC 
GATATTGAGA AAGCAGTGGA GTTAATGTGC CTTGTCCCCA AGCGCTGTAA TGACATGATG 
AATCTGGGAC GCCTGCAGGG CTTCGAGGGC ACCCTGACCG CCCAGGGCAA GCTACTGCAG 
CAGGACACAT TCTATGTGAT CGAGCTGGAT GCCGGCATGC AGTCCCGGAC CAAGGAGAGG 
CGTGTGTTTC TCTTTGAGCA GATTGTCATC TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC 
ACCCCAGGCT ACATGTTCAA AAGAAGCATC AAGATGAATT ACTTGGTCCT GGAAGACAAT 
GTGGATGGTG ACCCCTGCAA ATTTGCACTC ATGAACAGGG AGACTTCAGA AAGGGTCATT 
CTACAGGCTG CCAACTCAGA CATCCAGCAG GCCTGGGTGC AGGACATCAA CCAAGTCCTA 
GAAACACAGA GGGACTTCTT AAATGCACTA CAGTCACCCA TTGAGTATCA GCGGAAGGAG 
AGAAGCACAG CTGTGATCCG GTCCCAGCCC CCGAGGGTTC CACAAGCCAG CCCCAGGCCC 
TACTCTTCTG GCCCGGTGGG CTCAGAGAAG CCCCCCAAGG GCTCCAGCTA TAACCCGCCT 
CTGCCACCCC TGAAGATATC TACCTCTAAT GGCAGTCCAG GGTTCGACTA CCATCAGCCT 
GGGGACAAGT TTGACGCTAG CAAGCAGAAC GACCTGGGAG GCTGCAATGG GACATCAACC 
ATGACTGTAA TCAAAGATTA CTATGCACTG AAGGAGAATG AAATCTGCGT GAGCCAAGGT 
GAGGTGGTAC AGGTCCTCGC CGTCAACCAG CAGAACATGT GTCTGGTGTA CCAGCCTGCC 
AGCGACCATT CCCCGGCAGC AGAGGGCTGG GTCCCAGGCA GCATCCTGGC GCCCCTCGCC 
AAAGCCACAG CAGCGGCCGA AAGTAGTGAC GGGAGCATCA AGAAGTCATG CTCTTGGCAC 
ACTCTACGCA TGAGAAAGCG GGCAGACGTG GAGAACTCGG GTAAAAATGA AGCCACAGGG 
CCTCGTAAAC CCAAGGATAT TCTGGGCAAC AAAGTCTCTG TTAAAGAGAC GAACAGTTCC 
GAGGAGTCAG AGTGTGATGA TCTTGACCCT AATACTAGTA TGGAGATCTT GAATCCAAAT 
TTCATCCAGG AAGTGGCTCC AGAATTCCTC GTGCCCTTAG TAGATGTGAC CTGTTTGCTT 
GGAGACACAG TGCTACTGCA GTGTAAAGCC TGTGGGCGGC CAAAGCCCAG CATCACCTGG 
AAGGGTCCGG ATCAGAACAT CCTAGACACT GACAACAGTT CAGCAACATA CACCATCTCC 
TCCTGTGATT CTGGGGAAAG CACCCTCAAG ATCTGCAACC TGATGCCCCA GGATAGTGGG 
ATTTATACCT GCATAGCAGC AAACGACCAC GGGACTGCAT CCACATCTGC TACAGTTAAG 
GTGCAAGGTG TCCCCGCAGC CCCCAACCGC CCAATCGCAC AGGAGAGGAG CTGCACCTCC 
GTCATCCTCC GCTGGCTGCC CCCAGCCAGC ACCGGAAACT GCACTATTTC GGGGTACACA 
GTGGAGTATC GCGAGGAAGG TTCCCAGGTC TGGCAGCAAT CCGTAGCTTC GACCTTGGAC 
ACTTACCTGG TCATTGAAGA CCTTAGTCCT GGGTGCCCTT ATCAGTTCAG GGTCAGTGCC 
AGCAACCCCT GGGGAATCAG CCTTCCCAGT GAGCCCTCGG AGTTTGTGCG CCTTCCAGAG 
TACGATGCAG CCGCCGACGG TGCCACCATT TCTTGGAAGG AAAATTTTGA TTCTGCTTAC 
ACTGAACTGA ACGAGATTGG CAGAGGCCGT TTCTCTATTG TAAAGAAATG CATTCACAAA 
GCTACCCGCA AAGATGTTGC TGTGAAATTT GTGAGCAAAA AAATGAAGAA GAAGGAGCAG 
GCTGCTCACG AGGCCGCCCT GCTCCAGCAC CTGCAGCACC CTCAGTATGT CACGCTCCAT 
GACACCTACG AGTCCCCCAC ATCCTACATC CTGATTTTGG AACTGATGGA TGACGGCCGA 
CTCTTGGACT ACCTTATGAA TCATGATGAG CTGATGGAAG AAAAGGTAGC TTTCTACATT 
CGAGACATCA TGGAAGCTCT GCAGTACCTG CACAACTGTA GGGTCGCACA TTTGGACATA 
AAGCCCGAAA ACCTGCTCAT TGACCTCCGG ATCCCAGTGC CTCGAGTGAA GCTCATCGAC 
TTGGAAGACG CTGTCCAGAT CTCAGGCCAC TTCCATATCC ACCATCTGCT GGGGAACCCT 
GAGTTCGCTG CCCCCGAAGT GATCCAAGGC ATCCCTGTCT CCCTGGGCAC AGACATCTGG 
AGCATCGGGG TTCTGACATA TGTCATGCTT AGTGGGGTCT CCCCCTTCTT AGATGAGAGC 
AAAGAGGAAA CCTGTATCAA CGTGTGCAGG GTGGACTTTA GCTTCCCCCA CGAGTACTTC 
TGCGGTGTGA GCAATGCTGC CAGGGATTTC ATCAACGTAA TCTTACAGGA AGACTTCCGG 
AGGCGGCCCA CAGCAGCCAC GTGCCTGCAG CATCCCTGGC TACAGCCCCA CAACGGCAGC 
TACTCCAAGA TCCCCTTGGA CACCTCCCGT CTGGCCTGCT TCATAGAACG CCGCAAGCAC 
CAGAATGATG TGCGGCCCAT TCCCAATGTC AAGAGTTACA TCGTCAATAG GGTGAACCAA 
GGGACCTCGC TATCCCACAA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244262.1
CDS	24875..33781
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245960.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245960.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGACTC TGGAAGGAGG CTCTTACCGG GGGAGCTTGA AGGACCCTAC AGGCTGCCTG 
AATGAGGGGA TGACCCCTCC CACACCTCCT AGAAACCTGG AAGAAGAACA GAAAGCCAAG 
GCGCTGAGAG GCAGGATGTT TGTCCTGAAT GAGCTGGTAC AGACAGAGAA GGACTACGTC 
AAGGACCTGG GGATTGTGGT GGAGGGCTTC ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT 
GAGGACATGC GAGGGAAGGA AAAGATCGTG TTTGGAAATA TCCACCAGAT TTACGACTGG 
CATAAAGATT TTTTCCTGGC TGAACTGGAA AAATGTATCC AAGAGCAAGA CAGATTGGCC 
CAGCTCTTCA TTAAACACGA GCGGAAGCTG CACATCTACG TGTGGTACTG CCAGAACAAG 
CCGCGTTCCG AGTACATCGT GGCTGAGTAC GATGCCTATT TTGAGGAGGT TAAACAAGAG 
ATCAATCAAA GGCTGACCCT TAGTGACTTC CTGATCAAGC CCATTCAGAG AATAACAAAG 
TACCAGCTGC TACTCAAGGA CTTCCTGCGA TACAGCGAGA AGGCTGGTTT GGAGTGTTCC 
GATATTGAGA AAGCAGTGGA GTTAATGTGC CTTGTCCCCA AGCGCTGTAA TGACATGATG 
AATCTGGGAC GCCTGCAGGG CTTCGAGGGC ACCCTGACCG CCCAGGGCAA GCTACTGCAG 
CAGGACACAT TCTATGTGAT CGAGCTGGAT GCCGGCATGC AGTCCCGGAC CAAGGAGAGG 
CGTGTGTTTC TCTTTGAGCA GATTGTCATC TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC 
ACCCCAGGCT ACATGTTCAA AAGAAGCATC AAGATGAATT ACTTGGTCCT GGAAGACAAT 
GTGGATGGTG ACCCCTGCAA ATTTGCACTC ATGAACAGGG AGACTTCAGA AAGGGTCATT 
CTACAGGCTG CCAACTCAGA CATCCAGCAG GCCTGGGTGC AGGACATCAA CCAAGTCCTA 
GAAACACAGA GGGACTTCTT AAATGCACTA CAGTCACCCA TTGAGTATCA GCGGAAGGAG 
AGAAGCACAG CTGTGATCCG GTCCCAGCCC CCGAGGGTTC CACAAGCCAG CCCCAGGCCC 
TACTCTTCTG GCCCGGTGGG CTCAGAGAAG CCCCCCAAGG GCTCCAGCTA TAACCCGCCT 
CTGCCACCCC TGAAGATATC TACCTCTAAT GGCAGTCCAG GGTTCGACTA CCATCAGCCT 
GGGGACAAGT TTGACGCTAG CAAGCAGAAC GACCTGGGAG GCTGCAATGG GACATCAACC 
ATGACTGTAA TCAAAGATTA CTATGCACTG AAGGAGAATG AAATCTGCGT GAGCCAAGGT 
GAGGTGGTAC AGGTCCTCGC CGTCAACCAG CAGAACATGT GTCTGGTGTA CCAGCCTGCC 
AGCGACCATT CCCCGGCAGC AGAGGGCTGG GTCCCAGGCA GCATCCTGGC GCCCCTCGCC 
AAAGCCACAG CAGCGGCCGA AAGTAGTGAC GGGAGCATCA AGAAGTCATG CTCTTGGCAC 
ACTCTACGCA TGAGAAAGCG GGCAGACGTG GAGAACTCGG GTAAAAATGA AGCCACAGGG 
CCTCGTAAAC CCAAGGATAT TCTGGGCAAC AAAGTCTCTG TTAAAGAGAC GAACAGTTCC 
GAGGAGTCAG AGTGTGATGA TCTTGACCCT AATACTAGTA TGGAGATCTT GAATCCAAAT 
TTCATCCAGG AAGTGGCTCC AGAATTCCTC GTGCCCTTAG TAGATGTGAC CTGTTTGCTT 
GGAGACACAG TGCTACTGCA GTGTAAAGCC TGTGGGCGGC CAAAGCCCAG CATCACCTGG 
AAGGGTCCGG ATCAGAACAT CCTAGACACT GACAACAGTT CAGCAACATA CACCATCTCC 
TCCTGTGATT CTGGGGAAAG CACCCTCAAG ATCTGCAACC TGATGCCCCA GGATAGTGGG 
ATTTATACCT GCATAGCAGC AAACGACCAC GGGACTGCAT CCACATCTGC TACAGTTAAG 
GTGCAAGGTG TCCCCGCAGC CCCCAACCGC CCAATCGCAC AGGAGAGGAG CTGCACCTCC 
GTCATCCTCC GCTGGCTGCC CCCAGCCAGC ACCGGAAACT GCACTATTTC GGGGTACACA 
GTGGAGTATC GCGAGGAAGG TTCCCAGGTC TGGCAGCAAT CCGTAGCTTC GACCTTGGAC 
ACTTACCTGG TCATTGAAGA CCTTAGTCCT GGGTGCCCTT ATCAGTTCAG GGTCAGTGCC 
AGCAACCCCT GGGGAATCAG CCTTCCCAGT GAGCCCTCGG AGTTTGTGCG CCTTCCAGAG 
TACGATGCAG CCGCCGACGG TGCCACCATT TCTTGGAAGG AAAATTTTGA TTCTGCTTAC 
ACTGAACTGA ACGAGATTGG CAGAGGCCGT TTCTCTATTG TAAAGAAATG CATTCACAAA 
GCTACCCGCA AAGATGTTGC TGTGAAATTT GTGAGCAAAA AAATGAAGAA GAAGGAGCAG 
GCTGCTCACG AGGCCGCCCT GCTCCAGCAC CTGCAGCACC CTCAGTATGT CACGCTCCAT 
GACACCTACG AGTCCCCCAC ATCCTACATC CTGATTTTGG AACTGATGGA TGACGGCCGA 
CTCTTGGACT ACCTTATGAA TCATGATGAG CTGATGGAAG AAAAGGTAGC TTTCTACATT 
CGAGACATCA TGGAAGCTCT GCAGTACCTG CACAACTGTA GGGTCGCACA TTTGGACATA 
AAGCCCGAAA ACCTGCTCAT TGACCTCCGG ATCCCAGTGC CTCGAGTGAA GCTCATCGAC 
TTGGAAGACG CTGTCCAGAT CTCAGGCCAC TTCCATATCC ACCATCTGCT GGGGAACCCT 
GAGTTCGCTG CCCCCGAAGT GATCCAAGGC ATCCCTGTCT CCCTGGGCAC AGACATCTGG 
AGCATCGGGG TTCTGACATA TGTCATGCTT AGTGGGGTCT CCCCCTTCTT AGATGAGAGC 
AAAGAGGAAA CCTGTATCAA CGTGTGCAGG GTGGACTTTA GCTTCCCCCA CGAGTACTTC 
TGCGGTGTGA GCAATGCTGC CAGGGATTTC ATCAACGTAA TCTTACAGGA AGACTTCCGG 
AGGCGGCCCA CAGCAGCCAC GTGCCTGCAG CATCCCTGGC TACAGCCCCA CAACGGCAGC 
TACTCCAAGA TCCCCTTGGA CACCTCCCGT CTGGCCTGCT TCATAGAACG CCGCAAGCAC 
CAGAATGATG TGCGGCCCAT TCCCAATGTC AAGAGTTACA TCGTCAATAG GGTGAACCAA 
GGGACCTCGC TATCCCACAA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245961.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244263.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X14, mRNA.

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CloneID	OMu75316
Clone ID Related Accession (Same CDS sequence)	XM_011245961.3 , XM_011245961.2
Accession Version	XM_011245961.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245961.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGACTCTGG AAGGAGGCTC TTACCGGGGG AGCTTGAAGG ACCCTACAGG CTGCCTGAAT 
GAGGGGATGA CCCCTCCCAC ACCTCCTAGA AACCTGGAAG AAGAACAGAA AGCCAAGGCG 
CTGAGAGGCA GGATGTTTGT CCTGAATGAG CTGGTACAGA CAGAGAAGGA CTACGTCAAG 
GACCTGGGGA TTGTGGTGGA GGGCTTCATG AAGAGAATAG AAGAGAAGGG GGTCCCTGAG 
GACATGCGAG GGAAGGAAAA GATCGTGTTT GGAAATATCC ACCAGATTTA CGACTGGCAT 
AAAGATTTTT TCCTGGCTGA ACTGGAAAAA TGTATCCAAG AGCAAGACAG ATTGGCCCAG 
CTCTTCATTA AACACGAGCG GAAGCTGCAC ATCTACGTGT GGTACTGCCA GAACAAGCCG 
CGTTCCGAGT ACATCGTGGC TGAGTACGAT GCCTATTTTG AGGAGGTTAA ACAAGAGATC 
AATCAAAGGC TGACCCTTAG TGACTTCCTG ATCAAGCCCA TTCAGAGAAT AACAAAGTAC 
CAGCTGCTAC TCAAGGACTT CCTGCGATAC AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT 
ATTGAGAAAG CAGTGGAGTT AATGTGCCTT GTCCCCAAGC GCTGTAATGA CATGATGAAT 
CTGGGACGCC TGCAGGGCTT CGAGGGCACC CTGACCGCCC AGGGCAAGCT ACTGCAGCAG 
GACACATTCT ATGTGATCGA GCTGGATGCC GGCATGCAGT CCCGGACCAA GGAGAGGCGT 
GTGTTTCTCT TTGAGCAGAT TGTCATCTTC AGTGAACTGC TCCGAAAGGG GTCCCTCACC 
CCAGGCTACA TGTTCAAAAG AAGCATCAAG ATGAATTACT TGGTCCTGGA AGACAATGTG 
GATGGTGACC CCTGCAAATT TGCACTCATG AACAGGGAGA CTTCAGAAAG GGTCATTCTA 
CAGGCTGCCA ACTCAGACAT CCAGCAGGCC TGGGTGCAGG ACATCAACCA AGTCCTAGAA 
ACACAGAGGG ACTTCTTAAA TGCACTACAG TCACCCATTG AGTATCAGCG GAAGGAGAGA 
AGCACAGCTG TGATCCGGTC CCAGCCCCCG AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC 
TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC CCCAAGGGCT CCAGCTATAA CCCGCCTCTG 
CCACCCCTGA AGATATCTAC CTCTAATGGC AGTCCAGGGT TCGACTACCA TCAGCCTGGG 
GACAAGTTTG ACGCTAGCAA GCAGAACGAC CTGGGAGGCT GCAATGGGAC ATCAACCATG 
ACTGTAATCA AAGATTACTA TGCACTGAAG GAGAATGAAA TCTGCGTGAG CCAAGGTGAG 
GTGGTACAGG TCCTCGCCGT CAACCAGCAG AACATGTGTC TGGTGTACCA GCCTGCCAGC 
GACCATTCCC CGGCAGCAGA GGGCTGGGTC CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA 
GCCACAGCAG CGGCCGAAAG TAGTGACGGG AGCATCAAGA AGTCATGCTC TTGGCACACT 
CTACGCATGA GAAAGCGGGC AGACGTGGAG AACTCGGGTA AAAATGAAGC CACAGGGCCT 
CGTAAACCCA AGGATATTCT GGGCAACAAA GTCTCTGTTA AAGAGACGAA CAGTTCCGAG 
GAGTCAGAGT GTGATGATCT TGACCCTAAT ACTAGTATGG AGATCTTGAA TCCAAATTTC 
ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG CCCTTAGTAG ATGTGACCTG TTTGCTTGGA 
GACACAGTGC TACTGCAGTG TAAAGCCTGT GGGCGGCCAA AGCCCAGCAT CACCTGGAAG 
GGTCCGGATC AGAACATCCT AGACACTGAC AACAGTTCAG CAACATACAC CATCTCCTCC 
TGTGATTCTG GGGAAAGCAC CCTCAAGATC TGCAACCTGA TGCCCCAGGA TAGTGGGATT 
TATACCTGCA TAGCAGCAAA CGACCACGGG ACTGCATCCA CATCTGCTAC AGTTAAGGTG 
CAAGGTGTCC CCGCAGCCCC CAACCGCCCA ATCGCACAGG AGAGGAGCTG CACCTCCGTC 
ATCCTCCGCT GGCTGCCCCC AGCCAGCACC GGAAACTGCA CTATTTCGGG GTACACAGTG 
GAGTATCGCG AGGAAGGTTC CCAGGTCTGG CAGCAATCCG TAGCTTCGAC CTTGGACACT 
TACCTGGTCA TTGAAGACCT TAGTCCTGGG TGCCCTTATC AGTTCAGGGT CAGTGCCAGC 
AACCCCTGGG GAATCAGCCT TCCCAGTGAG CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC 
GATGCAGCCG CCGACGGTGC CACCATTTCT TGGAAGGAAA ATTTTGATTC TGCTTACACT 
GAACTGAACG AGATTGGCAG AGGCCGTTTC TCTATTGTAA AGAAATGCAT TCACAAAGCT 
ACCCGCAAAG ATGTTGCTGT GAAATTTGTG AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT 
GCTCACGAGG CCGCCCTGCT CCAGCACCTG CAGCACCCTC AGTATGTCAC GCTCCATGAC 
ACCTACGAGT CCCCCACATC CTACATCCTG ATTTTGGAAC TGATGGATGA CGGCCGACTC 
TTGGACTACC TTATGAATCA TGATGAGCTG ATGGAAGAAA AGGTAGCTTT CTACATTCGA 
GACATCATGG AAGCTCTGCA GTACCTGCAC AACTGTAGGG TCGCACATTT GGACATAAAG 
CCCGAAAACC TGCTCATTGA CCTCCGGATC CCAGTGCCTC GAGTGAAGCT CATCGACTTG 
GAAGACGCTG TCCAGATCTC AGGCCACTTC CATATCCACC ATCTGCTGGG GAACCCTGAG 
TTCGCTGCCC CCGAAGTGAT CCAAGGCATC CCTGTCTCCC TGGGCACAGA CATCTGGAGC 
ATCGGGGTTC TGACATATGT CATGCTTAGT GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA 
GAGGAAACCT GTATCAACGT GTGCAGGGTG GACTTTAGCT TCCCCCACGA GTACTTCTGC 
GGTGTGAGCA ATGCTGCCAG GGATTTCATC AACGTAATCT TACAGGAAGA CTTCCGGAGG 
CGGCCCACAG CAGCCACGTG CCTGCAGCAT CCCTGGCTAC AGCCCCACAA CGGCAGCTAC 
TCCAAGATCC CCTTGGACAC CTCCCGTCTG GCCTGCTTCA TAGAACGCCG CAAGCACCAG 
AATGATGTGC GGCCCATTCC CAATGTCAAG AGTTACATCG TCAATAGGGT GAACCAAGGG 
ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244263.1
CDS	24875..33778
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245961.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245961.3

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGACTCTGG AAGGAGGCTC TTACCGGGGG AGCTTGAAGG ACCCTACAGG CTGCCTGAAT 
GAGGGGATGA CCCCTCCCAC ACCTCCTAGA AACCTGGAAG AAGAACAGAA AGCCAAGGCG 
CTGAGAGGCA GGATGTTTGT CCTGAATGAG CTGGTACAGA CAGAGAAGGA CTACGTCAAG 
GACCTGGGGA TTGTGGTGGA GGGCTTCATG AAGAGAATAG AAGAGAAGGG GGTCCCTGAG 
GACATGCGAG GGAAGGAAAA GATCGTGTTT GGAAATATCC ACCAGATTTA CGACTGGCAT 
AAAGATTTTT TCCTGGCTGA ACTGGAAAAA TGTATCCAAG AGCAAGACAG ATTGGCCCAG 
CTCTTCATTA AACACGAGCG GAAGCTGCAC ATCTACGTGT GGTACTGCCA GAACAAGCCG 
CGTTCCGAGT ACATCGTGGC TGAGTACGAT GCCTATTTTG AGGAGGTTAA ACAAGAGATC 
AATCAAAGGC TGACCCTTAG TGACTTCCTG ATCAAGCCCA TTCAGAGAAT AACAAAGTAC 
CAGCTGCTAC TCAAGGACTT CCTGCGATAC AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT 
ATTGAGAAAG CAGTGGAGTT AATGTGCCTT GTCCCCAAGC GCTGTAATGA CATGATGAAT 
CTGGGACGCC TGCAGGGCTT CGAGGGCACC CTGACCGCCC AGGGCAAGCT ACTGCAGCAG 
GACACATTCT ATGTGATCGA GCTGGATGCC GGCATGCAGT CCCGGACCAA GGAGAGGCGT 
GTGTTTCTCT TTGAGCAGAT TGTCATCTTC AGTGAACTGC TCCGAAAGGG GTCCCTCACC 
CCAGGCTACA TGTTCAAAAG AAGCATCAAG ATGAATTACT TGGTCCTGGA AGACAATGTG 
GATGGTGACC CCTGCAAATT TGCACTCATG AACAGGGAGA CTTCAGAAAG GGTCATTCTA 
CAGGCTGCCA ACTCAGACAT CCAGCAGGCC TGGGTGCAGG ACATCAACCA AGTCCTAGAA 
ACACAGAGGG ACTTCTTAAA TGCACTACAG TCACCCATTG AGTATCAGCG GAAGGAGAGA 
AGCACAGCTG TGATCCGGTC CCAGCCCCCG AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC 
TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC CCCAAGGGCT CCAGCTATAA CCCGCCTCTG 
CCACCCCTGA AGATATCTAC CTCTAATGGC AGTCCAGGGT TCGACTACCA TCAGCCTGGG 
GACAAGTTTG ACGCTAGCAA GCAGAACGAC CTGGGAGGCT GCAATGGGAC ATCAACCATG 
ACTGTAATCA AAGATTACTA TGCACTGAAG GAGAATGAAA TCTGCGTGAG CCAAGGTGAG 
GTGGTACAGG TCCTCGCCGT CAACCAGCAG AACATGTGTC TGGTGTACCA GCCTGCCAGC 
GACCATTCCC CGGCAGCAGA GGGCTGGGTC CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA 
GCCACAGCAG CGGCCGAAAG TAGTGACGGG AGCATCAAGA AGTCATGCTC TTGGCACACT 
CTACGCATGA GAAAGCGGGC AGACGTGGAG AACTCGGGTA AAAATGAAGC CACAGGGCCT 
CGTAAACCCA AGGATATTCT GGGCAACAAA GTCTCTGTTA AAGAGACGAA CAGTTCCGAG 
GAGTCAGAGT GTGATGATCT TGACCCTAAT ACTAGTATGG AGATCTTGAA TCCAAATTTC 
ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG CCCTTAGTAG ATGTGACCTG TTTGCTTGGA 
GACACAGTGC TACTGCAGTG TAAAGCCTGT GGGCGGCCAA AGCCCAGCAT CACCTGGAAG 
GGTCCGGATC AGAACATCCT AGACACTGAC AACAGTTCAG CAACATACAC CATCTCCTCC 
TGTGATTCTG GGGAAAGCAC CCTCAAGATC TGCAACCTGA TGCCCCAGGA TAGTGGGATT 
TATACCTGCA TAGCAGCAAA CGACCACGGG ACTGCATCCA CATCTGCTAC AGTTAAGGTG 
CAAGGTGTCC CCGCAGCCCC CAACCGCCCA ATCGCACAGG AGAGGAGCTG CACCTCCGTC 
ATCCTCCGCT GGCTGCCCCC AGCCAGCACC GGAAACTGCA CTATTTCGGG GTACACAGTG 
GAGTATCGCG AGGAAGGTTC CCAGGTCTGG CAGCAATCCG TAGCTTCGAC CTTGGACACT 
TACCTGGTCA TTGAAGACCT TAGTCCTGGG TGCCCTTATC AGTTCAGGGT CAGTGCCAGC 
AACCCCTGGG GAATCAGCCT TCCCAGTGAG CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC 
GATGCAGCCG CCGACGGTGC CACCATTTCT TGGAAGGAAA ATTTTGATTC TGCTTACACT 
GAACTGAACG AGATTGGCAG AGGCCGTTTC TCTATTGTAA AGAAATGCAT TCACAAAGCT 
ACCCGCAAAG ATGTTGCTGT GAAATTTGTG AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT 
GCTCACGAGG CCGCCCTGCT CCAGCACCTG CAGCACCCTC AGTATGTCAC GCTCCATGAC 
ACCTACGAGT CCCCCACATC CTACATCCTG ATTTTGGAAC TGATGGATGA CGGCCGACTC 
TTGGACTACC TTATGAATCA TGATGAGCTG ATGGAAGAAA AGGTAGCTTT CTACATTCGA 
GACATCATGG AAGCTCTGCA GTACCTGCAC AACTGTAGGG TCGCACATTT GGACATAAAG 
CCCGAAAACC TGCTCATTGA CCTCCGGATC CCAGTGCCTC GAGTGAAGCT CATCGACTTG 
GAAGACGCTG TCCAGATCTC AGGCCACTTC CATATCCACC ATCTGCTGGG GAACCCTGAG 
TTCGCTGCCC CCGAAGTGAT CCAAGGCATC CCTGTCTCCC TGGGCACAGA CATCTGGAGC 
ATCGGGGTTC TGACATATGT CATGCTTAGT GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA 
GAGGAAACCT GTATCAACGT GTGCAGGGTG GACTTTAGCT TCCCCCACGA GTACTTCTGC 
GGTGTGAGCA ATGCTGCCAG GGATTTCATC AACGTAATCT TACAGGAAGA CTTCCGGAGG 
CGGCCCACAG CAGCCACGTG CCTGCAGCAT CCCTGGCTAC AGCCCCACAA CGGCAGCTAC 
TCCAAGATCC CCTTGGACAC CTCCCGTCTG GCCTGCTTCA TAGAACGCCG CAAGCACCAG 
AATGATGTGC GGCCCATTCC CAATGTCAAG AGTTACATCG TCAATAGGGT GAACCAAGGG 
ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_011245961.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244263.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X14, mRNA.

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CloneID	OMu75316
Clone ID Related Accession (Same CDS sequence)	XM_011245961.3 , XM_011245961.2
Accession Version	XM_011245961.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGACTCTGG AAGGAGGCTC TTACCGGGGG AGCTTGAAGG ACCCTACAGG CTGCCTGAAT 
GAGGGGATGA CCCCTCCCAC ACCTCCTAGA AACCTGGAAG AAGAACAGAA AGCCAAGGCG 
CTGAGAGGCA GGATGTTTGT CCTGAATGAG CTGGTACAGA CAGAGAAGGA CTACGTCAAG 
GACCTGGGGA TTGTGGTGGA GGGCTTCATG AAGAGAATAG AAGAGAAGGG GGTCCCTGAG 
GACATGCGAG GGAAGGAAAA GATCGTGTTT GGAAATATCC ACCAGATTTA CGACTGGCAT 
AAAGATTTTT TCCTGGCTGA ACTGGAAAAA TGTATCCAAG AGCAAGACAG ATTGGCCCAG 
CTCTTCATTA AACACGAGCG GAAGCTGCAC ATCTACGTGT GGTACTGCCA GAACAAGCCG 
CGTTCCGAGT ACATCGTGGC TGAGTACGAT GCCTATTTTG AGGAGGTTAA ACAAGAGATC 
AATCAAAGGC TGACCCTTAG TGACTTCCTG ATCAAGCCCA TTCAGAGAAT AACAAAGTAC 
CAGCTGCTAC TCAAGGACTT CCTGCGATAC AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT 
ATTGAGAAAG CAGTGGAGTT AATGTGCCTT GTCCCCAAGC GCTGTAATGA CATGATGAAT 
CTGGGACGCC TGCAGGGCTT CGAGGGCACC CTGACCGCCC AGGGCAAGCT ACTGCAGCAG 
GACACATTCT ATGTGATCGA GCTGGATGCC GGCATGCAGT CCCGGACCAA GGAGAGGCGT 
GTGTTTCTCT TTGAGCAGAT TGTCATCTTC AGTGAACTGC TCCGAAAGGG GTCCCTCACC 
CCAGGCTACA TGTTCAAAAG AAGCATCAAG ATGAATTACT TGGTCCTGGA AGACAATGTG 
GATGGTGACC CCTGCAAATT TGCACTCATG AACAGGGAGA CTTCAGAAAG GGTCATTCTA 
CAGGCTGCCA ACTCAGACAT CCAGCAGGCC TGGGTGCAGG ACATCAACCA AGTCCTAGAA 
ACACAGAGGG ACTTCTTAAA TGCACTACAG TCACCCATTG AGTATCAGCG GAAGGAGAGA 
AGCACAGCTG TGATCCGGTC CCAGCCCCCG AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC 
TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC CCCAAGGGCT CCAGCTATAA CCCGCCTCTG 
CCACCCCTGA AGATATCTAC CTCTAATGGC AGTCCAGGGT TCGACTACCA TCAGCCTGGG 
GACAAGTTTG ACGCTAGCAA GCAGAACGAC CTGGGAGGCT GCAATGGGAC ATCAACCATG 
ACTGTAATCA AAGATTACTA TGCACTGAAG GAGAATGAAA TCTGCGTGAG CCAAGGTGAG 
GTGGTACAGG TCCTCGCCGT CAACCAGCAG AACATGTGTC TGGTGTACCA GCCTGCCAGC 
GACCATTCCC CGGCAGCAGA GGGCTGGGTC CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA 
GCCACAGCAG CGGCCGAAAG TAGTGACGGG AGCATCAAGA AGTCATGCTC TTGGCACACT 
CTACGCATGA GAAAGCGGGC AGACGTGGAG AACTCGGGTA AAAATGAAGC CACAGGGCCT 
CGTAAACCCA AGGATATTCT GGGCAACAAA GTCTCTGTTA AAGAGACGAA CAGTTCCGAG 
GAGTCAGAGT GTGATGATCT TGACCCTAAT ACTAGTATGG AGATCTTGAA TCCAAATTTC 
ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG CCCTTAGTAG ATGTGACCTG TTTGCTTGGA 
GACACAGTGC TACTGCAGTG TAAAGCCTGT GGGCGGCCAA AGCCCAGCAT CACCTGGAAG 
GGTCCGGATC AGAACATCCT AGACACTGAC AACAGTTCAG CAACATACAC CATCTCCTCC 
TGTGATTCTG GGGAAAGCAC CCTCAAGATC TGCAACCTGA TGCCCCAGGA TAGTGGGATT 
TATACCTGCA TAGCAGCAAA CGACCACGGG ACTGCATCCA CATCTGCTAC AGTTAAGGTG 
CAAGGTGTCC CCGCAGCCCC CAACCGCCCA ATCGCACAGG AGAGGAGCTG CACCTCCGTC 
ATCCTCCGCT GGCTGCCCCC AGCCAGCACC GGAAACTGCA CTATTTCGGG GTACACAGTG 
GAGTATCGCG AGGAAGGTTC CCAGGTCTGG CAGCAATCCG TAGCTTCGAC CTTGGACACT 
TACCTGGTCA TTGAAGACCT TAGTCCTGGG TGCCCTTATC AGTTCAGGGT CAGTGCCAGC 
AACCCCTGGG GAATCAGCCT TCCCAGTGAG CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC 
GATGCAGCCG CCGACGGTGC CACCATTTCT TGGAAGGAAA ATTTTGATTC TGCTTACACT 
GAACTGAACG AGATTGGCAG AGGCCGTTTC TCTATTGTAA AGAAATGCAT TCACAAAGCT 
ACCCGCAAAG ATGTTGCTGT GAAATTTGTG AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT 
GCTCACGAGG CCGCCCTGCT CCAGCACCTG CAGCACCCTC AGTATGTCAC GCTCCATGAC 
ACCTACGAGT CCCCCACATC CTACATCCTG ATTTTGGAAC TGATGGATGA CGGCCGACTC 
TTGGACTACC TTATGAATCA TGATGAGCTG ATGGAAGAAA AGGTAGCTTT CTACATTCGA 
GACATCATGG AAGCTCTGCA GTACCTGCAC AACTGTAGGG TCGCACATTT GGACATAAAG 
CCCGAAAACC TGCTCATTGA CCTCCGGATC CCAGTGCCTC GAGTGAAGCT CATCGACTTG 
GAAGACGCTG TCCAGATCTC AGGCCACTTC CATATCCACC ATCTGCTGGG GAACCCTGAG 
TTCGCTGCCC CCGAAGTGAT CCAAGGCATC CCTGTCTCCC TGGGCACAGA CATCTGGAGC 
ATCGGGGTTC TGACATATGT CATGCTTAGT GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA 
GAGGAAACCT GTATCAACGT GTGCAGGGTG GACTTTAGCT TCCCCCACGA GTACTTCTGC 
GGTGTGAGCA ATGCTGCCAG GGATTTCATC AACGTAATCT TACAGGAAGA CTTCCGGAGG 
CGGCCCACAG CAGCCACGTG CCTGCAGCAT CCCTGGCTAC AGCCCCACAA CGGCAGCTAC 
TCCAAGATCC CCTTGGACAC CTCCCGTCTG GCCTGCTTCA TAGAACGCCG CAAGCACCAG 
AATGATGTGC GGCCCATTCC CAATGTCAAG AGTTACATCG TCAATAGGGT GAACCAAGGG 
ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244263.1
CDS	4891..13794
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADESKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_011245961.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245961.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAGCAAGAAG GGTTGGGGCG AAGACGAGCC AGATGAAGAG 
TCACACACAC CCCTCCCTCC GCCCATGAAG ATCTTTGACA ACGACCCCAC ACAGGATGAG 
ATGACTCTGG AAGGAGGCTC TTACCGGGGG AGCTTGAAGG ACCCTACAGG CTGCCTGAAT 
GAGGGGATGA CCCCTCCCAC ACCTCCTAGA AACCTGGAAG AAGAACAGAA AGCCAAGGCG 
CTGAGAGGCA GGATGTTTGT CCTGAATGAG CTGGTACAGA CAGAGAAGGA CTACGTCAAG 
GACCTGGGGA TTGTGGTGGA GGGCTTCATG AAGAGAATAG AAGAGAAGGG GGTCCCTGAG 
GACATGCGAG GGAAGGAAAA GATCGTGTTT GGAAATATCC ACCAGATTTA CGACTGGCAT 
AAAGATTTTT TCCTGGCTGA ACTGGAAAAA TGTATCCAAG AGCAAGACAG ATTGGCCCAG 
CTCTTCATTA AACACGAGCG GAAGCTGCAC ATCTACGTGT GGTACTGCCA GAACAAGCCG 
CGTTCCGAGT ACATCGTGGC TGAGTACGAT GCCTATTTTG AGGAGGTTAA ACAAGAGATC 
AATCAAAGGC TGACCCTTAG TGACTTCCTG ATCAAGCCCA TTCAGAGAAT AACAAAGTAC 
CAGCTGCTAC TCAAGGACTT CCTGCGATAC AGCGAGAAGG CTGGTTTGGA GTGTTCCGAT 
ATTGAGAAAG CAGTGGAGTT AATGTGCCTT GTCCCCAAGC GCTGTAATGA CATGATGAAT 
CTGGGACGCC TGCAGGGCTT CGAGGGCACC CTGACCGCCC AGGGCAAGCT ACTGCAGCAG 
GACACATTCT ATGTGATCGA GCTGGATGCC GGCATGCAGT CCCGGACCAA GGAGAGGCGT 
GTGTTTCTCT TTGAGCAGAT TGTCATCTTC AGTGAACTGC TCCGAAAGGG GTCCCTCACC 
CCAGGCTACA TGTTCAAAAG AAGCATCAAG ATGAATTACT TGGTCCTGGA AGACAATGTG 
GATGGTGACC CCTGCAAATT TGCACTCATG AACAGGGAGA CTTCAGAAAG GGTCATTCTA 
CAGGCTGCCA ACTCAGACAT CCAGCAGGCC TGGGTGCAGG ACATCAACCA AGTCCTAGAA 
ACACAGAGGG ACTTCTTAAA TGCACTACAG TCACCCATTG AGTATCAGCG GAAGGAGAGA 
AGCACAGCTG TGATCCGGTC CCAGCCCCCG AGGGTTCCAC AAGCCAGCCC CAGGCCCTAC 
TCTTCTGGCC CGGTGGGCTC AGAGAAGCCC CCCAAGGGCT CCAGCTATAA CCCGCCTCTG 
CCACCCCTGA AGATATCTAC CTCTAATGGC AGTCCAGGGT TCGACTACCA TCAGCCTGGG 
GACAAGTTTG ACGCTAGCAA GCAGAACGAC CTGGGAGGCT GCAATGGGAC ATCAACCATG 
ACTGTAATCA AAGATTACTA TGCACTGAAG GAGAATGAAA TCTGCGTGAG CCAAGGTGAG 
GTGGTACAGG TCCTCGCCGT CAACCAGCAG AACATGTGTC TGGTGTACCA GCCTGCCAGC 
GACCATTCCC CGGCAGCAGA GGGCTGGGTC CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA 
GCCACAGCAG CGGCCGAAAG TAGTGACGGG AGCATCAAGA AGTCATGCTC TTGGCACACT 
CTACGCATGA GAAAGCGGGC AGACGTGGAG AACTCGGGTA AAAATGAAGC CACAGGGCCT 
CGTAAACCCA AGGATATTCT GGGCAACAAA GTCTCTGTTA AAGAGACGAA CAGTTCCGAG 
GAGTCAGAGT GTGATGATCT TGACCCTAAT ACTAGTATGG AGATCTTGAA TCCAAATTTC 
ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG CCCTTAGTAG ATGTGACCTG TTTGCTTGGA 
GACACAGTGC TACTGCAGTG TAAAGCCTGT GGGCGGCCAA AGCCCAGCAT CACCTGGAAG 
GGTCCGGATC AGAACATCCT AGACACTGAC AACAGTTCAG CAACATACAC CATCTCCTCC 
TGTGATTCTG GGGAAAGCAC CCTCAAGATC TGCAACCTGA TGCCCCAGGA TAGTGGGATT 
TATACCTGCA TAGCAGCAAA CGACCACGGG ACTGCATCCA CATCTGCTAC AGTTAAGGTG 
CAAGGTGTCC CCGCAGCCCC CAACCGCCCA ATCGCACAGG AGAGGAGCTG CACCTCCGTC 
ATCCTCCGCT GGCTGCCCCC AGCCAGCACC GGAAACTGCA CTATTTCGGG GTACACAGTG 
GAGTATCGCG AGGAAGGTTC CCAGGTCTGG CAGCAATCCG TAGCTTCGAC CTTGGACACT 
TACCTGGTCA TTGAAGACCT TAGTCCTGGG TGCCCTTATC AGTTCAGGGT CAGTGCCAGC 
AACCCCTGGG GAATCAGCCT TCCCAGTGAG CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC 
GATGCAGCCG CCGACGGTGC CACCATTTCT TGGAAGGAAA ATTTTGATTC TGCTTACACT 
GAACTGAACG AGATTGGCAG AGGCCGTTTC TCTATTGTAA AGAAATGCAT TCACAAAGCT 
ACCCGCAAAG ATGTTGCTGT GAAATTTGTG AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT 
GCTCACGAGG CCGCCCTGCT CCAGCACCTG CAGCACCCTC AGTATGTCAC GCTCCATGAC 
ACCTACGAGT CCCCCACATC CTACATCCTG ATTTTGGAAC TGATGGATGA CGGCCGACTC 
TTGGACTACC TTATGAATCA TGATGAGCTG ATGGAAGAAA AGGTAGCTTT CTACATTCGA 
GACATCATGG AAGCTCTGCA GTACCTGCAC AACTGTAGGG TCGCACATTT GGACATAAAG 
CCCGAAAACC TGCTCATTGA CCTCCGGATC CCAGTGCCTC GAGTGAAGCT CATCGACTTG 
GAAGACGCTG TCCAGATCTC AGGCCACTTC CATATCCACC ATCTGCTGGG GAACCCTGAG 
TTCGCTGCCC CCGAAGTGAT CCAAGGCATC CCTGTCTCCC TGGGCACAGA CATCTGGAGC 
ATCGGGGTTC TGACATATGT CATGCTTAGT GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA 
GAGGAAACCT GTATCAACGT GTGCAGGGTG GACTTTAGCT TCCCCCACGA GTACTTCTGC 
GGTGTGAGCA ATGCTGCCAG GGATTTCATC AACGTAATCT TACAGGAAGA CTTCCGGAGG 
CGGCCCACAG CAGCCACGTG CCTGCAGCAT CCCTGGCTAC AGCCCCACAA CGGCAGCTAC 
TCCAAGATCC CCTTGGACAC CTCCCGTCTG GCCTGCTTCA TAGAACGCCG CAAGCACCAG 
AATGATGTGC GGCCCATTCC CAATGTCAAG AGTTACATCG TCAATAGGGT GAACCAAGGG 
ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317071.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172560.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X15, mRNA.

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CloneID	OMu79369
Clone ID Related Accession (Same CDS sequence)	XM_017317071.1 , XM_017317071.2
Accession Version	XM_017317071.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kalirin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGACTC TGGAAGGAGG CTCTTACCGG GGGAGCTTGA AGGACCCTAC AGGCTGCCTG 
AATGAGGGGA TGACCCCTCC CACACCTCCT AGAAACCTGG AAGAAGAACA GAAAGCCAAG 
GCGCTGAGAG GCAGGATGTT TGTCCTGAAT GAGCTGGTAC AGACAGAGAA GGACTACGTC 
AAGGACCTGG GGATTGTGGT GGAGGGCTTC ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT 
GAGGACATGC GAGGGAAGGA AAAGATCGTG TTTGGAAATA TCCACCAGAT TTACGACTGG 
CATAAAGATT TTTTCCTGGC TGAACTGGAA AAATGTATCC AAGAGCAAGA CAGATTGGCC 
CAGCTCTTCA TTAAACACGA GCGGAAGCTG CACATCTACG TGTGGTACTG CCAGAACAAG 
CCGCGTTCCG AGTACATCGT GGCTGAGTAC GATGCCTATT TTGAGGAGGT TAAACAAGAG 
ATCAATCAAA GGCTGACCCT TAGTGACTTC CTGATCAAGC CCATTCAGAG AATAACAAAG 
TACCAGCTGC TACTCAAGGA CTTCCTGCGA TACAGCGAGA AGGCTGGTTT GGAGTGTTCC 
GATATTGAGA AAGCAGTGGA GTTAATGTGC CTTGTCCCCA AGCGCTGTAA TGACATGATG 
AATCTGGGAC GCCTGCAGGG CTTCGAGGGC ACCCTGACCG CCCAGGGCAA GCTACTGCAG 
CAGGACACAT TCTATGTGAT CGAGCTGGAT GCCGGCATGC AGTCCCGGAC CAAGGAGAGG 
CGTGTGTTTC TCTTTGAGCA GATTGTCATC TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC 
ACCCCAGGCT ACATGTTCAA AAGAAGCATC AAGATGAATT ACTTGGTCCT GGAAGACAAT 
GTGGATGGTG ACCCCTGCAA ATTTGCACTC ATGAACAGGG AGACTTCAGA AAGGGTCATT 
CTACAGGCTG CCAACTCAGA CATCCAGCAG GCCTGGGTGC AGGACATCAA CCAAGTCCTA 
GAAACACAGA GGGACTTCTT AAATGCACTA CAGTCACCCA TTGAGTATCA GCGGAAGGAG 
AGAAGCACAG CTGTGATCCG GTCCCAGCCC CCGAGGGTTC CACAAGCCAG CCCCAGGCCC 
TACTCTTCTG GCCCGGTGGG CTCAGAGAAG CCCCCCAAGG GCTCCAGCTA TAACCCGCCT 
CTGCCACCCC TGAAGATATC TACCTCTAAT GGCAGTCCAG GGTTCGACTA CCATCAGCCT 
GGGGACAAGT TTGACGCTAG CAAGAACGAC CTGGGAGGCT GCAATGGGAC ATCAACCATG 
ACTGTAATCA AAGATTACTA TGCACTGAAG GAGAATGAAA TCTGCGTGAG CCAAGGTGAG 
GTGGTACAGG TCCTCGCCGT CAACCAGCAG AACATGTGTC TGGTGTACCA GCCTGCCAGC 
GACCATTCCC CGGCAGCAGA GGGCTGGGTC CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA 
GCCACAGCAG CGGCCGAAAG TAGTGACGGG AGCATCAAGA AGTCATGCTC TTGGCACACT 
CTACGCATGA GAAAGCGGGC AGACGTGGAG AACTCGGGTA AAAATGAAGC CACAGGGCCT 
CGTAAACCCA AGGATATTCT GGGCAACAAA GTCTCTGTTA AAGAGACGAA CAGTTCCGAG 
GAGTCAGAGT GTGATGATCT TGACCCTAAT ACTAGTATGG AGATCTTGAA TCCAAATTTC 
ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG CCCTTAGTAG ATGTGACCTG TTTGCTTGGA 
GACACAGTGC TACTGCAGTG TAAAGCCTGT GGGCGGCCAA AGCCCAGCAT CACCTGGAAG 
GGTCCGGATC AGAACATCCT AGACACTGAC AACAGTTCAG CAACATACAC CATCTCCTCC 
TGTGATTCTG GGGAAAGCAC CCTCAAGATC TGCAACCTGA TGCCCCAGGA TAGTGGGATT 
TATACCTGCA TAGCAGCAAA CGACCACGGG ACTGCATCCA CATCTGCTAC AGTTAAGGTG 
CAAGGTGTCC CCGCAGCCCC CAACCGCCCA ATCGCACAGG AGAGGAGCTG CACCTCCGTC 
ATCCTCCGCT GGCTGCCCCC AGCCAGCACC GGAAACTGCA CTATTTCGGG GTACACAGTG 
GAGTATCGCG AGGAAGGTTC CCAGGTCTGG CAGCAATCCG TAGCTTCGAC CTTGGACACT 
TACCTGGTCA TTGAAGACCT TAGTCCTGGG TGCCCTTATC AGTTCAGGGT CAGTGCCAGC 
AACCCCTGGG GAATCAGCCT TCCCAGTGAG CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC 
GATGCAGCCG CCGACGGTGC CACCATTTCT TGGAAGGAAA ATTTTGATTC TGCTTACACT 
GAACTGAACG AGATTGGCAG AGGCCGTTTC TCTATTGTAA AGAAATGCAT TCACAAAGCT 
ACCCGCAAAG ATGTTGCTGT GAAATTTGTG AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT 
GCTCACGAGG CCGCCCTGCT CCAGCACCTG CAGCACCCTC AGTATGTCAC GCTCCATGAC 
ACCTACGAGT CCCCCACATC CTACATCCTG ATTTTGGAAC TGATGGATGA CGGCCGACTC 
TTGGACTACC TTATGAATCA TGATGAGCTG ATGGAAGAAA AGGTAGCTTT CTACATTCGA 
GACATCATGG AAGCTCTGCA GTACCTGCAC AACTGTAGGG TCGCACATTT GGACATAAAG 
CCCGAAAACC TGCTCATTGA CCTCCGGATC CCAGTGCCTC GAGTGAAGCT CATCGACTTG 
GAAGACGCTG TCCAGATCTC AGGCCACTTC CATATCCACC ATCTGCTGGG GAACCCTGAG 
TTCGCTGCCC CCGAAGTGAT CCAAGGCATC CCTGTCTCCC TGGGCACAGA CATCTGGAGC 
ATCGGGGTTC TGACATATGT CATGCTTAGT GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA 
GAGGAAACCT GTATCAACGT GTGCAGGGTG GACTTTAGCT TCCCCCACGA GTACTTCTGC 
GGTGTGAGCA ATGCTGCCAG GGATTTCATC AACGTAATCT TACAGGAAGA CTTCCGGAGG 
CGGCCCACAG CAGCCACGTG CCTGCAGCAT CCCTGGCTAC AGCCCCACAA CGGCAGCTAC 
TCCAAGATCC CCTTGGACAC CTCCCGTCTG GCCTGCTTCA TAGAACGCCG CAAGCACCAG 
AATGATGTGC GGCCCATTCC CAATGTCAAG AGTTACATCG TCAATAGGGT GAACCAAGGG 
ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172560.1
CDS	4891..13794
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_017317071.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317071.1

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGACTC TGGAAGGAGG CTCTTACCGG GGGAGCTTGA AGGACCCTAC AGGCTGCCTG 
AATGAGGGGA TGACCCCTCC CACACCTCCT AGAAACCTGG AAGAAGAACA GAAAGCCAAG 
GCGCTGAGAG GCAGGATGTT TGTCCTGAAT GAGCTGGTAC AGACAGAGAA GGACTACGTC 
AAGGACCTGG GGATTGTGGT GGAGGGCTTC ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT 
GAGGACATGC GAGGGAAGGA AAAGATCGTG TTTGGAAATA TCCACCAGAT TTACGACTGG 
CATAAAGATT TTTTCCTGGC TGAACTGGAA AAATGTATCC AAGAGCAAGA CAGATTGGCC 
CAGCTCTTCA TTAAACACGA GCGGAAGCTG CACATCTACG TGTGGTACTG CCAGAACAAG 
CCGCGTTCCG AGTACATCGT GGCTGAGTAC GATGCCTATT TTGAGGAGGT TAAACAAGAG 
ATCAATCAAA GGCTGACCCT TAGTGACTTC CTGATCAAGC CCATTCAGAG AATAACAAAG 
TACCAGCTGC TACTCAAGGA CTTCCTGCGA TACAGCGAGA AGGCTGGTTT GGAGTGTTCC 
GATATTGAGA AAGCAGTGGA GTTAATGTGC CTTGTCCCCA AGCGCTGTAA TGACATGATG 
AATCTGGGAC GCCTGCAGGG CTTCGAGGGC ACCCTGACCG CCCAGGGCAA GCTACTGCAG 
CAGGACACAT TCTATGTGAT CGAGCTGGAT GCCGGCATGC AGTCCCGGAC CAAGGAGAGG 
CGTGTGTTTC TCTTTGAGCA GATTGTCATC TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC 
ACCCCAGGCT ACATGTTCAA AAGAAGCATC AAGATGAATT ACTTGGTCCT GGAAGACAAT 
GTGGATGGTG ACCCCTGCAA ATTTGCACTC ATGAACAGGG AGACTTCAGA AAGGGTCATT 
CTACAGGCTG CCAACTCAGA CATCCAGCAG GCCTGGGTGC AGGACATCAA CCAAGTCCTA 
GAAACACAGA GGGACTTCTT AAATGCACTA CAGTCACCCA TTGAGTATCA GCGGAAGGAG 
AGAAGCACAG CTGTGATCCG GTCCCAGCCC CCGAGGGTTC CACAAGCCAG CCCCAGGCCC 
TACTCTTCTG GCCCGGTGGG CTCAGAGAAG CCCCCCAAGG GCTCCAGCTA TAACCCGCCT 
CTGCCACCCC TGAAGATATC TACCTCTAAT GGCAGTCCAG GGTTCGACTA CCATCAGCCT 
GGGGACAAGT TTGACGCTAG CAAGAACGAC CTGGGAGGCT GCAATGGGAC ATCAACCATG 
ACTGTAATCA AAGATTACTA TGCACTGAAG GAGAATGAAA TCTGCGTGAG CCAAGGTGAG 
GTGGTACAGG TCCTCGCCGT CAACCAGCAG AACATGTGTC TGGTGTACCA GCCTGCCAGC 
GACCATTCCC CGGCAGCAGA GGGCTGGGTC CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA 
GCCACAGCAG CGGCCGAAAG TAGTGACGGG AGCATCAAGA AGTCATGCTC TTGGCACACT 
CTACGCATGA GAAAGCGGGC AGACGTGGAG AACTCGGGTA AAAATGAAGC CACAGGGCCT 
CGTAAACCCA AGGATATTCT GGGCAACAAA GTCTCTGTTA AAGAGACGAA CAGTTCCGAG 
GAGTCAGAGT GTGATGATCT TGACCCTAAT ACTAGTATGG AGATCTTGAA TCCAAATTTC 
ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG CCCTTAGTAG ATGTGACCTG TTTGCTTGGA 
GACACAGTGC TACTGCAGTG TAAAGCCTGT GGGCGGCCAA AGCCCAGCAT CACCTGGAAG 
GGTCCGGATC AGAACATCCT AGACACTGAC AACAGTTCAG CAACATACAC CATCTCCTCC 
TGTGATTCTG GGGAAAGCAC CCTCAAGATC TGCAACCTGA TGCCCCAGGA TAGTGGGATT 
TATACCTGCA TAGCAGCAAA CGACCACGGG ACTGCATCCA CATCTGCTAC AGTTAAGGTG 
CAAGGTGTCC CCGCAGCCCC CAACCGCCCA ATCGCACAGG AGAGGAGCTG CACCTCCGTC 
ATCCTCCGCT GGCTGCCCCC AGCCAGCACC GGAAACTGCA CTATTTCGGG GTACACAGTG 
GAGTATCGCG AGGAAGGTTC CCAGGTCTGG CAGCAATCCG TAGCTTCGAC CTTGGACACT 
TACCTGGTCA TTGAAGACCT TAGTCCTGGG TGCCCTTATC AGTTCAGGGT CAGTGCCAGC 
AACCCCTGGG GAATCAGCCT TCCCAGTGAG CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC 
GATGCAGCCG CCGACGGTGC CACCATTTCT TGGAAGGAAA ATTTTGATTC TGCTTACACT 
GAACTGAACG AGATTGGCAG AGGCCGTTTC TCTATTGTAA AGAAATGCAT TCACAAAGCT 
ACCCGCAAAG ATGTTGCTGT GAAATTTGTG AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT 
GCTCACGAGG CCGCCCTGCT CCAGCACCTG CAGCACCCTC AGTATGTCAC GCTCCATGAC 
ACCTACGAGT CCCCCACATC CTACATCCTG ATTTTGGAAC TGATGGATGA CGGCCGACTC 
TTGGACTACC TTATGAATCA TGATGAGCTG ATGGAAGAAA AGGTAGCTTT CTACATTCGA 
GACATCATGG AAGCTCTGCA GTACCTGCAC AACTGTAGGG TCGCACATTT GGACATAAAG 
CCCGAAAACC TGCTCATTGA CCTCCGGATC CCAGTGCCTC GAGTGAAGCT CATCGACTTG 
GAAGACGCTG TCCAGATCTC AGGCCACTTC CATATCCACC ATCTGCTGGG GAACCCTGAG 
TTCGCTGCCC CCGAAGTGAT CCAAGGCATC CCTGTCTCCC TGGGCACAGA CATCTGGAGC 
ATCGGGGTTC TGACATATGT CATGCTTAGT GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA 
GAGGAAACCT GTATCAACGT GTGCAGGGTG GACTTTAGCT TCCCCCACGA GTACTTCTGC 
GGTGTGAGCA ATGCTGCCAG GGATTTCATC AACGTAATCT TACAGGAAGA CTTCCGGAGG 
CGGCCCACAG CAGCCACGTG CCTGCAGCAT CCCTGGCTAC AGCCCCACAA CGGCAGCTAC 
TCCAAGATCC CCTTGGACAC CTCCCGTCTG GCCTGCTTCA TAGAACGCCG CAAGCACCAG 
AATGATGTGC GGCCCATTCC CAATGTCAAG AGTTACATCG TCAATAGGGT GAACCAAGGG 
ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kalrn ( XM_017317071.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172560.1 Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X15, mRNA.

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CloneID	OMu79369
Clone ID Related Accession (Same CDS sequence)	XM_017317071.1 , XM_017317071.2
Accession Version	XM_017317071.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kalirin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317071.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGACTC TGGAAGGAGG CTCTTACCGG GGGAGCTTGA AGGACCCTAC AGGCTGCCTG 
AATGAGGGGA TGACCCCTCC CACACCTCCT AGAAACCTGG AAGAAGAACA GAAAGCCAAG 
GCGCTGAGAG GCAGGATGTT TGTCCTGAAT GAGCTGGTAC AGACAGAGAA GGACTACGTC 
AAGGACCTGG GGATTGTGGT GGAGGGCTTC ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT 
GAGGACATGC GAGGGAAGGA AAAGATCGTG TTTGGAAATA TCCACCAGAT TTACGACTGG 
CATAAAGATT TTTTCCTGGC TGAACTGGAA AAATGTATCC AAGAGCAAGA CAGATTGGCC 
CAGCTCTTCA TTAAACACGA GCGGAAGCTG CACATCTACG TGTGGTACTG CCAGAACAAG 
CCGCGTTCCG AGTACATCGT GGCTGAGTAC GATGCCTATT TTGAGGAGGT TAAACAAGAG 
ATCAATCAAA GGCTGACCCT TAGTGACTTC CTGATCAAGC CCATTCAGAG AATAACAAAG 
TACCAGCTGC TACTCAAGGA CTTCCTGCGA TACAGCGAGA AGGCTGGTTT GGAGTGTTCC 
GATATTGAGA AAGCAGTGGA GTTAATGTGC CTTGTCCCCA AGCGCTGTAA TGACATGATG 
AATCTGGGAC GCCTGCAGGG CTTCGAGGGC ACCCTGACCG CCCAGGGCAA GCTACTGCAG 
CAGGACACAT TCTATGTGAT CGAGCTGGAT GCCGGCATGC AGTCCCGGAC CAAGGAGAGG 
CGTGTGTTTC TCTTTGAGCA GATTGTCATC TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC 
ACCCCAGGCT ACATGTTCAA AAGAAGCATC AAGATGAATT ACTTGGTCCT GGAAGACAAT 
GTGGATGGTG ACCCCTGCAA ATTTGCACTC ATGAACAGGG AGACTTCAGA AAGGGTCATT 
CTACAGGCTG CCAACTCAGA CATCCAGCAG GCCTGGGTGC AGGACATCAA CCAAGTCCTA 
GAAACACAGA GGGACTTCTT AAATGCACTA CAGTCACCCA TTGAGTATCA GCGGAAGGAG 
AGAAGCACAG CTGTGATCCG GTCCCAGCCC CCGAGGGTTC CACAAGCCAG CCCCAGGCCC 
TACTCTTCTG GCCCGGTGGG CTCAGAGAAG CCCCCCAAGG GCTCCAGCTA TAACCCGCCT 
CTGCCACCCC TGAAGATATC TACCTCTAAT GGCAGTCCAG GGTTCGACTA CCATCAGCCT 
GGGGACAAGT TTGACGCTAG CAAGAACGAC CTGGGAGGCT GCAATGGGAC ATCAACCATG 
ACTGTAATCA AAGATTACTA TGCACTGAAG GAGAATGAAA TCTGCGTGAG CCAAGGTGAG 
GTGGTACAGG TCCTCGCCGT CAACCAGCAG AACATGTGTC TGGTGTACCA GCCTGCCAGC 
GACCATTCCC CGGCAGCAGA GGGCTGGGTC CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA 
GCCACAGCAG CGGCCGAAAG TAGTGACGGG AGCATCAAGA AGTCATGCTC TTGGCACACT 
CTACGCATGA GAAAGCGGGC AGACGTGGAG AACTCGGGTA AAAATGAAGC CACAGGGCCT 
CGTAAACCCA AGGATATTCT GGGCAACAAA GTCTCTGTTA AAGAGACGAA CAGTTCCGAG 
GAGTCAGAGT GTGATGATCT TGACCCTAAT ACTAGTATGG AGATCTTGAA TCCAAATTTC 
ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG CCCTTAGTAG ATGTGACCTG TTTGCTTGGA 
GACACAGTGC TACTGCAGTG TAAAGCCTGT GGGCGGCCAA AGCCCAGCAT CACCTGGAAG 
GGTCCGGATC AGAACATCCT AGACACTGAC AACAGTTCAG CAACATACAC CATCTCCTCC 
TGTGATTCTG GGGAAAGCAC CCTCAAGATC TGCAACCTGA TGCCCCAGGA TAGTGGGATT 
TATACCTGCA TAGCAGCAAA CGACCACGGG ACTGCATCCA CATCTGCTAC AGTTAAGGTG 
CAAGGTGTCC CCGCAGCCCC CAACCGCCCA ATCGCACAGG AGAGGAGCTG CACCTCCGTC 
ATCCTCCGCT GGCTGCCCCC AGCCAGCACC GGAAACTGCA CTATTTCGGG GTACACAGTG 
GAGTATCGCG AGGAAGGTTC CCAGGTCTGG CAGCAATCCG TAGCTTCGAC CTTGGACACT 
TACCTGGTCA TTGAAGACCT TAGTCCTGGG TGCCCTTATC AGTTCAGGGT CAGTGCCAGC 
AACCCCTGGG GAATCAGCCT TCCCAGTGAG CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC 
GATGCAGCCG CCGACGGTGC CACCATTTCT TGGAAGGAAA ATTTTGATTC TGCTTACACT 
GAACTGAACG AGATTGGCAG AGGCCGTTTC TCTATTGTAA AGAAATGCAT TCACAAAGCT 
ACCCGCAAAG ATGTTGCTGT GAAATTTGTG AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT 
GCTCACGAGG CCGCCCTGCT CCAGCACCTG CAGCACCCTC AGTATGTCAC GCTCCATGAC 
ACCTACGAGT CCCCCACATC CTACATCCTG ATTTTGGAAC TGATGGATGA CGGCCGACTC 
TTGGACTACC TTATGAATCA TGATGAGCTG ATGGAAGAAA AGGTAGCTTT CTACATTCGA 
GACATCATGG AAGCTCTGCA GTACCTGCAC AACTGTAGGG TCGCACATTT GGACATAAAG 
CCCGAAAACC TGCTCATTGA CCTCCGGATC CCAGTGCCTC GAGTGAAGCT CATCGACTTG 
GAAGACGCTG TCCAGATCTC AGGCCACTTC CATATCCACC ATCTGCTGGG GAACCCTGAG 
TTCGCTGCCC CCGAAGTGAT CCAAGGCATC CCTGTCTCCC TGGGCACAGA CATCTGGAGC 
ATCGGGGTTC TGACATATGT CATGCTTAGT GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA 
GAGGAAACCT GTATCAACGT GTGCAGGGTG GACTTTAGCT TCCCCCACGA GTACTTCTGC 
GGTGTGAGCA ATGCTGCCAG GGATTTCATC AACGTAATCT TACAGGAAGA CTTCCGGAGG 
CGGCCCACAG CAGCCACGTG CCTGCAGCAT CCCTGGCTAC AGCCCCACAA CGGCAGCTAC 
TCCAAGATCC CCTTGGACAC CTCCCGTCTG GCCTGCTTCA TAGAACGCCG CAAGCACCAG 
AATGATGTGC GGCCCATTCC CAATGTCAAG AGTTACATCG TCAATAGGGT GAACCAAGGG 
ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172560.1
CDS	24875..33778
Translation	MCGLHLPVSCTYLRSVDSAAERSEVPVVLGGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNIREVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVESDKLSGGCELTVVLQDFSAGHSSELSIQVGQTVELLERPSERPGWCLVRTTERSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPASTGNCTISGYTVEYREEGSQVWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWKENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYVTLHDTYESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPVPRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEETCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLACFIERRKHQNDVRPIPNVKSYIVNRVNQGTSLSHNP

Target ORF information:

RefSeq Version	XM_017317071.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kalirin, RhoGEF kinase (Kalrn), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317071.2

ORF Insert Sequence:

ATGTGTGGCC TTCACTTGCC AGTTAGCTGC ACGTATCTGA GGAGCGTGGA TAGTGCCGCG 
GAGAGATCAG AGGTCCCAGT TGTCCTGGGA GGATCTTTTC GGAATGATGG TTTGAAAGCT 
TCTGATGTCC TTCCTATCCT AAAGGAGAAA GTGGCCTTTG TGTCTGGGGG GCGTGACAAG 
CGAGGTGGAC CCATCCTGAC CTTCCCTGCC CGAAGCAATC ATGACAGAAT AAGACAGGAA 
GACCTACGGA AACTTGTGAC GTATTTGGCC AGCGTGCCAA GTGAGGACGT TTGCAAACGT 
GGCTTCACCG TCATCATCGA CATGCGGGGC TCCAAGTGGG ATCTCATCAA GCCCCTCCTC 
AAGACGCTGC AGGAAGCCTT CCCCGCCGAG ATCCATGTGG CCCTCATCAT CAAGCCTGAC 
AACTTCTGGC AGAAGCAGAA GACCAACTTT GGCAGCTCCA AATTCATCTT TGAGACAAGC 
ATGGTGTCTG TGGAGGGCCT CACGAAGCTG GTGGACCCCT CCCAGCTGAC AGAGGAGTTT 
GACGGCTCCC TGGATTACAA CCATGAGGAG TGGATTGAGT TGCGCCTCTC CCTGGAGGAG 
TTCTTCAACA GTGCGGTCCA CCTGCTCTCA CGCCTTGAGG ACCTGCAGGA GATGCTGGCC 
CGGAAGGAGT TCCCTGTGGA TGTGGAGGGC TCTCGGAGGC TTATCGACGA GCACACGCAA 
CTTAAGAAAA AGGTGCTGAA GGCCCCCGTG GAAGAGCTGG ACCGGGAGGG GCAGCGTCTA 
CTGCAGTGCA TCCGCTGCAG CGATGGCTTC TCAGGGCGCA ACTGCATCCC AGGAAGTGCC 
GACTTCCAGA GCCTTGTACC CAAGATCACC AGCCTCCTGG ACAAGCTGCA TTCCACCCGA 
CAACACCTGC ACCAAATGTG GCACGTGCGA AAGCTCAAGC TGGATCAGTG CTTCCAGCTG 
CGGCTCTTCG AGCAGGATGC TGAGAAGATG TTCGACTGGA TAAGCCACAA CAAGGAGTTA 
TTTCTTCAGA GCCACACAGA GATCGGAGTC AGCTACCAGC ATGCACTAGA CCTCCAGACA 
CAGCACAATC ACTTTGCCAT GAACTCCATG AATGCCTATG TCAACATCAA CCGCATCATG 
TCTGTCGCTT CCCGCCTCTC AGAGGCCGGC CATTATGCCT CCCAGCAAAT CAAGCAGATC 
TCCACGCAGC TAGACCAGGA GTGGAAGAGC TTTGCCGCTG CCCTGGATGA ACGCAGTACA 
ATCCTGGCCA TGTCTGCTGT GTTCCACCAG AAGGCAGAGC AGTTCCTGTC AGGCGTGGAT 
GCCTGGTGTA AGATGTGCAG CGAAGGCGGC CTTCCTTCTG AGATGCAGGA CCTGGAGCTG 
GCAATCCACC ATCACCAGTC CTTATATGAG CAGGTGACCC AGGCCTACAC AGAGGTCAGC 
CAGGATGGCA AAGCACTACT GGATGTCCTG CAGCGTCCCC TGAGTCCTGG GAACTCTGAG 
TCCCTCACAG CCACAGCCAA CTACTCCAAG GCGGTCCACC AGGTGCTGGA TGTGGTCCAC 
GAGGTGCTGC ACCATCAGCG ACGGCTCGAG AGCATCTGGC AGCACCGCAA GGTGCGGCTT 
CACCAGCGGC TGCAGCTGTG TGTCTTCCAG CAGGATGTGC AGCAGGTGCT GGACTGGATT 
GAAAACCATG GCGAGGCCTT TCTCAGCAAA CACACTGGGG TTGGCAAGTC CTTGCATCGA 
GCACGTGCAC TACAGAAGAG GCATGATGAC TTTGAAGAAG TGGCACAGAA TACATACACC 
AATGCGGACA AGCTCCTAGA AGCAGCGGAG CAGCTGGCTC AGACTGGGGA ATGTGACCCG 
GAGGAGATCT ACAAGGCAGC CCGGCACCTG GAGGTGCGCA TCCAAGACTT TGTGCGCAGA 
GTGGAGCAGC GGAAGCTGCT TCTGGACATG TCGGTCTCTT TCCACACGCA CACCAAAGAG 
CTGTGGACAT GGATGGAGGA CCTTCAGAAG GAGGTGCTGG AGGATGTCTG TGCAGACTCT 
GTGGATGCGG TCCAGGAGCT GATCAAGCAG TTCCAGCAGC AGCAGACCGC CACCCTGGAT 
GCCACTCTCA ATGTCATCAA GGAAGGCGAA GACCTTATCC AGCAGCTCAG GTCAGCGCCT 
CCCTCCCTGG GGGAGCCCAC CGAGGCCAGG GACTCAGCGA TGTCCAACAA CAAGACACCC 
CACAGTAGCT CTATCAGCCA TATTGAGTCT GTCCTCCAGC AGCTGGACGA TGCTCAGGTA 
CAGATGGAAG AGCTGTTTCA CGAGCGGAAG ATCAAGCTGG ACATCTTCCT ACAGCTGCGC 
ATCTTTGAAC AGTACACCAT TGAGGTCACT GCAGAGCTGG ATGCCTGGAA CGAGGACTTG 
CTCCGGCAGA TGAATGATTT CAACACAGAG GATCTGACCC TGGCTGAGCA GCGGCTACAG 
CGCCACACAG AACGGAAGCT TGCCATGAAC AACATGACGT TCGAGGTCAT CCAGCAGGGC 
CAGGACCTGC ACCAGTACAT CATGGAAGTG CAAGCGTCAG GAATCGAGTT AATCTGTGAG 
AAGGACCTTG ACCTGGCAGC TCAGGTGCAG GAGCTGCTGG AGTTTCTCCA TGAGAAGCAG 
CACGAGCTGG AGCTCAATGC GGAGCAGACG CACAAGCGGC TAGAGCAGTG TCTCCAGCTG 
AGGCACCTCC AGGCTGAGGT CAAACAGGTT CTGGGATGGA TCCGCAATGG AGAGTCGATG 
CTCAACGCCA GCCTGGTCAA CGCCAGCTCT TTGTCGGAGG CAGAGCAGCT GCAGCGGGAG 
CATGAACAGT TCCAGCTAGC CATCGAGTCC CTCTTTCATG CCACTTCCTT GCAGAAGACG 
CACCAGAGCG CCCTGCAGGT ACAGCAGAAG GCAGAGGCAC TGCTCCAGGC CGGCCACTAC 
GACGCAGACG CCATCCGGGA ATGTGCTGAG AAGGTGGCCC TCCACTGGCA ACAGCTCATG 
CTAAAGATGG AGGACCGGCT GAAGCTTGTC AACGCCTCTG TGGCCTTTTA CAAGACCTCT 
GAGCAGGTGT GCAGTGTTCT GGAGAGCTTG GAGCAGGAGT ACCGGAGAGA CGAGGACTGG 
TGTGGTGGGA GAGACAAGCT GGGGCCGGCG GCAGAGATGG ACCACGTTAT TCCACTCCTC 
AGCAAGCACC TGGAGCAGAA GGAGGCCTTC CTGAAGGCCT GCACGCTGGC AAGGCGGAAC 
GCCGAGGTGT TCCTCAAATA CATCCACAGG AACAATGTGA GCATGCCTAG TGTTGCCAGC 
CACACCCGGG GGCCTGAACA GCAAGTAAAA GCCATCTTGA GCGAGCTCTT GCAGAGGGAG 
AATCGAGTCC TGCACTTCTG GACCTTGAAG AAGCGGCGGT TAGACCAGTG TCAGCAATAT 
GTGGTGTTTG AGCGCAGTGC TAAGCAGGCA CTGGACTGGA TCCAAGAAAC AGGAGAATAT 
TACCTCTCAA CACATACCTC CACCGGAGAG ACCACGGAGG AGACTCAGGA GCTGCTGAAA 
GAGTATGGAG AATTCAGGGT GCCTGCCAAG CAAACGAAGG AGAAGGTGAA ACTTCTGATC 
CAGCTAGCAG ACAGCTTTGT GGAAAAGGGC CACATCCACG CCACAGAGAT ACGGAAATGG 
GTGACCACGG TGGACAAGCA CTACCGCGAT TTCTCCCTGA GGATGGGGAA GTACCGGTAC 
TCGCTGGAGA AGGCGCTGGG GGTCAACACG GAGGATAACA AGGACCTAGA GCTGGACATC 
ATCCCAGCAA GCCTTTCGGA TCGGGAGGTC AAGCTCCGGG ACGCTAACCA TGAGATCAAT 
GAAGAGAAAC GGAAGTCAGC TCGAAAGAAA GAGTTCATTA TGGCTGAACT GCTCCAAACT 
GAGAAGGCTT ATGTAAGGGA CTTGCACGAG TGCTTGGAGA CCTACCTGTG GGAGATGACT 
AGTGGTGTGG AAGAGATACC CCCCGGCATC CTCAATAAGG AACATATCAT CTTCGGCAAC 
ATCCAGGAGA TCTATGACTT CCATAACAAC ATCTTCCTCA AAGAGCTGGA GAAGTACGAG 
CAGCTGCCTG AGGATGTGGG ACACTGCTTT GTTACCTGGG CAGACAAATT TCAGATGTAT 
GTCACCTACT GTAAAAACAA GCCTGATTCC AACCAGCTGA TCCTGGAGCA TGCGGGCACC 
TTCTTTGATG AGATCCAGCA GCGGCATGGC CTGGCCAACT CCATCTCCTC CTACCTAATT 
AAGCCTGTCC AGAGGGTTAC CAAGTACCAG CTGCTCCTGA AGGAACTTCT AACTTGCTGT 
GAAGAAGGGA AAGGAGAGCT GAAGGATGGC CTGGAGGTGA TGCTCAGTGT CCCCAAGAAA 
GCCAACGATG CCATGCATGT CAGCATGCTG GAAGGGTTCG ATGAAAACTT GGATGTACAG 
GGGGAGTTGA TTCTCCAGGA TGCCTTTCAA GTGTGGGACC CAAAGTCGCT GATCCGGAAG 
GGGCGGGAGC GCCATCTGTT CCTCTTTGAG ATCTCCTTGG TGTTTAGCAA AGAGATCAAA 
GACTCTTCAG GACACACGAA ATATGTTTAC AAGAACAAGC TACTGACCTC AGAGCTGGGT 
GTGACCGAGC ATGTAGAAGG TGACCCCTGC AAATTCGCCT TGTGGTCTGG GCGCACCCCA 
TCCTCAGACA ATAAAACAGT GCTCAAAGCT TCCAACATCG AAACCAAGCA GGAATGGATC 
AAGAACATTC GAGAAGTGAT TCAGGAGAGG ATCATTCATC TTAAAGGCGC TTTAAAGGAG 
CCAATTCAGC TCCCCAAAAC ACCAGCCAAA CTGAGGAACA ATAGTAAGAG GGATGGGGTA 
GAGGATGGTG ACAGCCAAGG GGATGGGAGC AGCCAGCCAG ATACCATATC CATTGCCTCC 
AGGACCTCTC AGAACACAGT GGAAAGTGAC AAGCTCTCAG GTGGATGTGA GCTGACCGTG 
GTCCTCCAGG ACTTCAGCGC AGGCCACAGC AGCGAGCTGA GCATCCAGGT GGGCCAGACG 
GTGGAGCTGC TGGAACGACC GAGTGAGAGG CCTGGCTGGT GTCTGGTCCG CACCACAGAG 
CGGAGCCCCC CACAGGAAGG CCTGGTACCC AGCAGCGCCC TGTGCATCTC ACACTCTCGC 
AGCAGCGTGG AAATGGACTG CTTCTTCCCC TTGAAAGATT CATACTCTCA TTCCTCCAGT 
GAGAATGGAG GCAAATCCGA GTCCGTGGCC CACCTGCAGT CTCAGCCCTC CCTGAACTCC 
ATCCACAGTT CCCCTGGTCC CAAGCGCTCC ACCAACACCC TCAAAAAGTG GCTGACCAGC 
CCCGTGCGCC GCCTCAACAG CGGAAAAGCC GACGGGAACA TCAAAAAGCA GAAGAAAGTC 
CGGGATGGGC GGAAGAGCTT CGATCTGGGA TCGCCCAAGC CTGGGGATGA GACGACACCT 
CAGGGAGACA GCGCTGACGA GAAGAGCAAG AAGGGTTGGG GCGAAGACGA GCCAGATGAA 
GAGTCACACA CACCCCTCCC TCCGCCCATG AAGATCTTTG ACAACGACCC CACACAGGAT 
GAGATGACTC TGGAAGGAGG CTCTTACCGG GGGAGCTTGA AGGACCCTAC AGGCTGCCTG 
AATGAGGGGA TGACCCCTCC CACACCTCCT AGAAACCTGG AAGAAGAACA GAAAGCCAAG 
GCGCTGAGAG GCAGGATGTT TGTCCTGAAT GAGCTGGTAC AGACAGAGAA GGACTACGTC 
AAGGACCTGG GGATTGTGGT GGAGGGCTTC ATGAAGAGAA TAGAAGAGAA GGGGGTCCCT 
GAGGACATGC GAGGGAAGGA AAAGATCGTG TTTGGAAATA TCCACCAGAT TTACGACTGG 
CATAAAGATT TTTTCCTGGC TGAACTGGAA AAATGTATCC AAGAGCAAGA CAGATTGGCC 
CAGCTCTTCA TTAAACACGA GCGGAAGCTG CACATCTACG TGTGGTACTG CCAGAACAAG 
CCGCGTTCCG AGTACATCGT GGCTGAGTAC GATGCCTATT TTGAGGAGGT TAAACAAGAG 
ATCAATCAAA GGCTGACCCT TAGTGACTTC CTGATCAAGC CCATTCAGAG AATAACAAAG 
TACCAGCTGC TACTCAAGGA CTTCCTGCGA TACAGCGAGA AGGCTGGTTT GGAGTGTTCC 
GATATTGAGA AAGCAGTGGA GTTAATGTGC CTTGTCCCCA AGCGCTGTAA TGACATGATG 
AATCTGGGAC GCCTGCAGGG CTTCGAGGGC ACCCTGACCG CCCAGGGCAA GCTACTGCAG 
CAGGACACAT TCTATGTGAT CGAGCTGGAT GCCGGCATGC AGTCCCGGAC CAAGGAGAGG 
CGTGTGTTTC TCTTTGAGCA GATTGTCATC TTCAGTGAAC TGCTCCGAAA GGGGTCCCTC 
ACCCCAGGCT ACATGTTCAA AAGAAGCATC AAGATGAATT ACTTGGTCCT GGAAGACAAT 
GTGGATGGTG ACCCCTGCAA ATTTGCACTC ATGAACAGGG AGACTTCAGA AAGGGTCATT 
CTACAGGCTG CCAACTCAGA CATCCAGCAG GCCTGGGTGC AGGACATCAA CCAAGTCCTA 
GAAACACAGA GGGACTTCTT AAATGCACTA CAGTCACCCA TTGAGTATCA GCGGAAGGAG 
AGAAGCACAG CTGTGATCCG GTCCCAGCCC CCGAGGGTTC CACAAGCCAG CCCCAGGCCC 
TACTCTTCTG GCCCGGTGGG CTCAGAGAAG CCCCCCAAGG GCTCCAGCTA TAACCCGCCT 
CTGCCACCCC TGAAGATATC TACCTCTAAT GGCAGTCCAG GGTTCGACTA CCATCAGCCT 
GGGGACAAGT TTGACGCTAG CAAGAACGAC CTGGGAGGCT GCAATGGGAC ATCAACCATG 
ACTGTAATCA AAGATTACTA TGCACTGAAG GAGAATGAAA TCTGCGTGAG CCAAGGTGAG 
GTGGTACAGG TCCTCGCCGT CAACCAGCAG AACATGTGTC TGGTGTACCA GCCTGCCAGC 
GACCATTCCC CGGCAGCAGA GGGCTGGGTC CCAGGCAGCA TCCTGGCGCC CCTCGCCAAA 
GCCACAGCAG CGGCCGAAAG TAGTGACGGG AGCATCAAGA AGTCATGCTC TTGGCACACT 
CTACGCATGA GAAAGCGGGC AGACGTGGAG AACTCGGGTA AAAATGAAGC CACAGGGCCT 
CGTAAACCCA AGGATATTCT GGGCAACAAA GTCTCTGTTA AAGAGACGAA CAGTTCCGAG 
GAGTCAGAGT GTGATGATCT TGACCCTAAT ACTAGTATGG AGATCTTGAA TCCAAATTTC 
ATCCAGGAAG TGGCTCCAGA ATTCCTCGTG CCCTTAGTAG ATGTGACCTG TTTGCTTGGA 
GACACAGTGC TACTGCAGTG TAAAGCCTGT GGGCGGCCAA AGCCCAGCAT CACCTGGAAG 
GGTCCGGATC AGAACATCCT AGACACTGAC AACAGTTCAG CAACATACAC CATCTCCTCC 
TGTGATTCTG GGGAAAGCAC CCTCAAGATC TGCAACCTGA TGCCCCAGGA TAGTGGGATT 
TATACCTGCA TAGCAGCAAA CGACCACGGG ACTGCATCCA CATCTGCTAC AGTTAAGGTG 
CAAGGTGTCC CCGCAGCCCC CAACCGCCCA ATCGCACAGG AGAGGAGCTG CACCTCCGTC 
ATCCTCCGCT GGCTGCCCCC AGCCAGCACC GGAAACTGCA CTATTTCGGG GTACACAGTG 
GAGTATCGCG AGGAAGGTTC CCAGGTCTGG CAGCAATCCG TAGCTTCGAC CTTGGACACT 
TACCTGGTCA TTGAAGACCT TAGTCCTGGG TGCCCTTATC AGTTCAGGGT CAGTGCCAGC 
AACCCCTGGG GAATCAGCCT TCCCAGTGAG CCCTCGGAGT TTGTGCGCCT TCCAGAGTAC 
GATGCAGCCG CCGACGGTGC CACCATTTCT TGGAAGGAAA ATTTTGATTC TGCTTACACT 
GAACTGAACG AGATTGGCAG AGGCCGTTTC TCTATTGTAA AGAAATGCAT TCACAAAGCT 
ACCCGCAAAG ATGTTGCTGT GAAATTTGTG AGCAAAAAAA TGAAGAAGAA GGAGCAGGCT 
GCTCACGAGG CCGCCCTGCT CCAGCACCTG CAGCACCCTC AGTATGTCAC GCTCCATGAC 
ACCTACGAGT CCCCCACATC CTACATCCTG ATTTTGGAAC TGATGGATGA CGGCCGACTC 
TTGGACTACC TTATGAATCA TGATGAGCTG ATGGAAGAAA AGGTAGCTTT CTACATTCGA 
GACATCATGG AAGCTCTGCA GTACCTGCAC AACTGTAGGG TCGCACATTT GGACATAAAG 
CCCGAAAACC TGCTCATTGA CCTCCGGATC CCAGTGCCTC GAGTGAAGCT CATCGACTTG 
GAAGACGCTG TCCAGATCTC AGGCCACTTC CATATCCACC ATCTGCTGGG GAACCCTGAG 
TTCGCTGCCC CCGAAGTGAT CCAAGGCATC CCTGTCTCCC TGGGCACAGA CATCTGGAGC 
ATCGGGGTTC TGACATATGT CATGCTTAGT GGGGTCTCCC CCTTCTTAGA TGAGAGCAAA 
GAGGAAACCT GTATCAACGT GTGCAGGGTG GACTTTAGCT TCCCCCACGA GTACTTCTGC 
GGTGTGAGCA ATGCTGCCAG GGATTTCATC AACGTAATCT TACAGGAAGA CTTCCGGAGG 
CGGCCCACAG CAGCCACGTG CCTGCAGCAT CCCTGGCTAC AGCCCCACAA CGGCAGCTAC 
TCCAAGATCC CCTTGGACAC CTCCCGTCTG GCCTGCTTCA TAGAACGCCG CAAGCACCAG 
AATGATGTGC GGCCCATTCC CAATGTCAAG AGTTACATCG TCAATAGGGT GAACCAAGGG 
ACCTCGCTAT CCCACAACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Kalirin reduction rescues psychosis-associated behavioral deficits in APPswe/PSEN1dE9 transgenic mice.
Neurobiology of aging5459-70(2017 Jun)
Krivinko JM,Erickson SL,Abrahamson EE,Wills ZP,Ikonomovic MD,Penzes P,Sweet RA

Using Kalirin conditional knockout mice to distinguish its role in dopamine receptor mediated behaviors.
BMC neuroscience18(1)45(2017 May)
LaRese TP,Yan Y,Eipper BA,Mains RE

Synaptic pruning in the female hippocampus is triggered at puberty by extrasynaptic GABAA receptors on dendritic spines.
eLife5(2016 05)
Afroz S,Parato J,Shen H,Smith SS

A role for Kalirin-7 in nociceptive sensitization via activity-dependent modulation of spinal synapses.
Nature communications66820(2015 Apr)
Lu J,Luo C,Bali KK,Xie RG,Mains RE,Eipper BA,Kuner R

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Kalrn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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