Cldn8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cldn8
Entrez Gene ID	54420
Full Name	claudin 8
Synonyms	AI648025
General protein information	Preferred Names claudin 8 Names claudin-8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 C3.3
Summary	This intronless gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a paracellular cation barrier. [provided by RefSeq, Aug 2010].

mRNA and Protein(s)

mRNA	Protein	Name
NM_018778.3	NP_061248.1	claudin-8

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLDN8	XP_531532.1
Rattus norvegicus (Norway rat)	Cldn8	NP_001032863.1
Danio rerio (zebrafish)	zgc:110333	NP_001017799.1
Homo sapiens (human)	CLDN8	NP_955360.1
	CLDN8	XP_001100214.1
Canis lupus familiaris (dog)	CLDN8	XP_005638865.1
Mus musculus (house mouse)	Cldn8	NP_061248.1
Gallus gallus (chicken)	CLDN8	XP_004938436.1
Danio rerio (zebrafish)	cldn8	NP_001003733.1
Danio rerio (zebrafish)	cldn17	NP_001104000.1
Bos taurus (cattle)	CLDN8	NP_001091565.1

Gene Ontology
Bibliography

Related articles in PubMed

KLHL3 regulates paracellular chloride transport in the kidney by ubiquitination of claudin-8.
Gong Y, Wang J, Yang J, Gonzales E, Perez R, Hou J
Proceedings of the National Academy of Sciences of the United States of America112(14)4340-5(2015 Apr)

GATA4 and GATA6 regulate intestinal epithelial cytodifferentiation during development.
Walker EM, Thompson CA, Battle MA
Developmental biology392(2)283-94(2014 Aug)

Identification of molecular compartments and genetic circuitry in the developing mammalian kidney.
Yu J, Valerius MT, Duah M, Staser K, Hansard JK, Guo JJ, McMahon J, Vaughan J, Faria D, Georgas K, Rumballe B, Ren Q, Krautzberger AM, Junker JP, Thiagarajan RD, Machanick P, Gray PA, van Oudenaarden A, Rowitch DH, Stiles CD, Ma Q, Grimmond SM, Bailey TL, Little MH, McMahon AP
Development (Cambridge, England)139(10)1863-73(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

KLHL3 regulates paracellular chloride transport in the kidney by ubiquitination of claudin-8
Title: KLHL3 regulates paracellular chloride transport in the kidney by ubiquitination of claudin-8.

data show that claudin-4 interacts with claudin-8 and that their association is required for the anion-selective paracellular pathway in the collecting duct
Title: Claudin-4 forms paracellular chloride channel in the kidney and requires claudin-8 for tight junction localization.

claudin-8 was found to be expressed primarily at the tight junction along the entire aldosterone-sensitive distal nephron and in the late segments of the thin descending limbs of long-looped nephrons
Title: Expression of claudin-7 and -8 along the mouse nephron.

Claudin-8 is expressed at the variety of epithelial tissues in inner ear including stria vascularis, Reissner's membrane, spiral limbus, vestibular sensory epithelia, and dark cell area.
Title: Expression patterns of claudins, tight junction adhesion molecules, in the inner ear.

The following Cldn8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cldn8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58435	NM_018778.3 Latest version!	Mus musculus claudin 8 (Cldn8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cldn8 ( NM_018778.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061248.1 Mus musculus claudin 8 (Cldn8), mRNA.

Price & Availability↑

CloneID	OMu58435
Clone ID Related Accession (Same CDS sequence)	NM_018778.3
Accession Version	NM_018778.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	claudin-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK133810.1 and BE133033.1. On Nov 15, 2007 this sequence version replaced NM_018778.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: AK133810.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAACCT ACGCTCTTCA AATGGCTGCA CTGGTGCTTG GTGGTGTTGG CATGGTGGGC 
ACGGTGGCTG TGACTATCAT GCCTCAGTGG AGAGTGTCTG CCTTCATCGA AAGTAACATT 
GTGGTGTTTG AGAACCGCTG GGAAGGCTTG TGGATGAATT GTATGAGGCA TGCCAACATC 
AGAATGCAGT GCAAGGTCTA CGACTCCCTG CTGGCTCTTA GTCCAGACCT CCAGGCATCC 
CGAGGACTGA TGTGTGCTGC GTCCGTCTTG GCTTTCTTGG CTTTCATGAC AGCCATCCTC 
GGAATGAAGT GCACCAGATG CACGGGGGAC GATGAGAACG TGAAGAGCCG CATCTTGCTG 
ACAGCCGGAA TCATCTTCTT CATCACCGGC TTGGTTGTGC TCATCCCTGT CAGCTGGGTT 
GCCAATTCCA TCATCAGAGA CTTCTACAAC CCACTGGTGG ATGTGGCCCT AAAGCGCGAG 
CTGGGAGAAG CCCTCTACAT AGGCTGGACC ACAGCGCTGG TGCTGATCGC TGGAGGAGCA 
CTGTTCTGTT GTGTGTTTTG TTGTACTGAA AGGAGCAACA GTTACAGGTA CTCGGTACCA 
TCCCATCGCA CCACTCAACG GAGTTTCCAC GCCGAAAAGA GATCTCCGAG CATATACTCC 
AAAAGTCAGT ATGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061248.1
CDS	149..826
Translation	MATYALQMAALVLGGVGMVGTVAVTIMPQWRVSAFIESNIVVFENRWEGLWMNCMRHANIRMQCKVYDSLLALSPDLQASRGLMCAASVLAFLAFMTAILGMKCTRCTGDDENVKSRILLTAGIIFFITGLVVLIPVSWVANSIIRDFYNPLVDVALKRELGEALYIGWTTALVLIAGGALFCCVFCCTERSNSYRYSVPSHRTTQRSFHAEKRSPSIYSKSQYV

Target ORF information:

RefSeq Version	NM_018778.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus claudin 8 (Cldn8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018778.3

ORF Insert Sequence:

ATGGCAACCT ACGCTCTTCA AATGGCTGCA CTGGTGCTTG GTGGTGTTGG CATGGTGGGC 
ACGGTGGCTG TGACTATCAT GCCTCAGTGG AGAGTGTCTG CCTTCATCGA AAGTAACATT 
GTGGTGTTTG AGAACCGCTG GGAAGGCTTG TGGATGAATT GTATGAGGCA TGCCAACATC 
AGAATGCAGT GCAAGGTCTA CGACTCCCTG CTGGCTCTTA GTCCAGACCT CCAGGCATCC 
CGAGGACTGA TGTGTGCTGC GTCCGTCTTG GCTTTCTTGG CTTTCATGAC AGCCATCCTC 
GGAATGAAGT GCACCAGATG CACGGGGGAC GATGAGAACG TGAAGAGCCG CATCTTGCTG 
ACAGCCGGAA TCATCTTCTT CATCACCGGC TTGGTTGTGC TCATCCCTGT CAGCTGGGTT 
GCCAATTCCA TCATCAGAGA CTTCTACAAC CCACTGGTGG ATGTGGCCCT AAAGCGCGAG 
CTGGGAGAAG CCCTCTACAT AGGCTGGACC ACAGCGCTGG TGCTGATCGC TGGAGGAGCA 
CTGTTCTGTT GTGTGTTTTG TTGTACTGAA AGGAGCAACA GTTACAGGTA CTCGGTACCA 
TCCCATCGCA CCACTCAACG GAGTTTCCAC GCCGAAAAGA GATCTCCGAG CATATACTCC 
AAAAGTCAGT ATGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

KLHL3 regulates paracellular chloride transport in the kidney by ubiquitination of claudin-8.
Proceedings of the National Academy of Sciences of the United States of America112(14)4340-5(2015 Apr)
Gong Y,Wang J,Yang J,Gonzales E,Perez R,Hou J

GATA4 and GATA6 regulate intestinal epithelial cytodifferentiation during development.
Developmental biology392(2)283-94(2014 Aug)
Walker EM,Thompson CA,Battle MA

Identification of molecular compartments and genetic circuitry in the developing mammalian kidney.
Development (Cambridge, England)139(10)1863-73(2012 May)
Yu J,Valerius MT,Duah M,Staser K,Hansard JK,Guo JJ,McMahon J,Vaughan J,Faria D,Georgas K,Rumballe B,Ren Q,Krautzberger AM,Junker JP,Thiagarajan RD,Machanick P,Gray PA,van Oudenaarden A,Rowitch DH,Stiles CD,Ma Q,Grimmond SM,Bailey TL,Little MH,McMahon AP

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Cldn8 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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