gRNAs
The following Spag5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spag5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu46620 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006533748.1 | |
| Accession Version | XM_006533748.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | sperm-associated antigen 5 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCAGGAAT TCAGAGGCCT TCTACAGGAG GCACAGACCC AACTGATAGG GCTTCACACA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006533811.1 |
| CDS | 286..2055 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_006533748.1 |
1 | ATGCAGGAAT TCAGAGGCCT TCTACAGGAG GCACAGACCC AACTGATAGG GCTTCACACA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu46619 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006533747.4 , XM_006533747.3 | |
| Accession Version | XM_006533747.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | sperm-associated antigen 5 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533747.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006533810.1 |
| CDS | 75..3203 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006533747.4 |
1 | ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu10784 | |
| Clone ID Related Accession (Same CDS sequence) | NM_017407.3 , NM_017407.2 | |
| Accession Version | NM_017407.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-16 | |
| Organism | Mus musculus(house mouse) | |
| Product | sperm-associated antigen 5 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591070.19. On May 1, 2018 this sequence version replaced NM_017407.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK076619.1, SRR1660815.255395.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_059103.1 |
| CDS | 70..3567 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_017407.3 |
1 | ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu10784 | |
| Clone ID Related Accession (Same CDS sequence) | NM_017407.3 , NM_017407.2 | |
| Accession Version | NM_017407.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-01 | |
| Organism | Mus musculus(house mouse) | |
| Product | sperm-associated antigen 5 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591070.19. On Aug 13, 2009 this sequence version replaced NM_017407.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052672.1, AK076619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_059103.1 |
| CDS | 70..3567 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_017407.2 |
1 | ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu46619 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006533747.4 , XM_006533747.3 | |
| Accession Version | XM_006533747.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | sperm-associated antigen 5 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533747.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006533810.1 |
| CDS | 101..3229 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006533747.3 |
1 | ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells. |
The mitotic spindle protein SPAG5/Astrin connects to the Usher protein network postmitotically. |
The role of Pax6 in regulating the orientation and mode of cell division of progenitors in the mouse cerebral cortex. |
Genes expressed in Atoh1 neuronal lineages arising from the r1/isthmus rhombic lip. |
