Pmm2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pmm2
Entrez Gene ID	54128
Full Name	phosphomannomutase 2
Synonyms	AI585868,C86848
General protein information	Preferred Names phosphomannomutase 2 Names phosphomannomutase 2 PMM 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006522360.3	XP_006522423.1	phosphomannomutase 2 isoform X1
NM_016881.2	NP_058577.1	phosphomannomutase 2
XM_006522360.4	XP_006522423.1	phosphomannomutase 2 isoform X1
NM_001362485.1	NP_001349414.1	phosphomannomutase 2 isoform 2
NM_016881.3	NP_058577.1	phosphomannomutase 2 isoform 1

Pathways from BioSystems

Homologies

	PMM2	XP_001102327.1
Bos taurus (cattle)	PMM2	NP_001030267.1
Oryza sativa (rice)	Os04g0682300	NP_001054295.1
Homo sapiens (human)	PMM2	NP_000294.1
Pan troglodytes (chimpanzee)	PMM2	XP_510805.3
Rattus norvegicus (Norway rat)	Pmm2	NP_001100443.1
Gallus gallus (chicken)	PMM2	NP_001026639.1
Kluyveromyces lactis	KLLA0D05709g	XP_453316.1
Eremothecium gossypii	AGOS_ABR236W	NP_983185.1
Schizosaccharomyces pombe (fission yeast)	pmm1	NP_594325.1
Arabidopsis thaliana (thale cress)	PMM	NP_182103.1
Caenorhabditis elegans (roundworm)	CELE_F52B11.2	NP_001255786.1
Saccharomyces cerevisiae (baker's yeast)	SEC53	NP_116609.1
Xenopus tropicalis (tropical clawed frog)	pmm1	NP_001106421.1
Canis lupus familiaris (dog)	PMM2	XP_547133.3
Mus musculus (house mouse)	Pmm2	NP_058577.1
Danio rerio (zebrafish)	pmm2	NP_956378.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011353	XP_309297.2
Magnaporthe oryzae (rice blast fungus)	MGG_01637	XP_003714625.1
Drosophila melanogaster (fruit fly)	CG10688	NP_648589.1
Neurospora crassa	NCU02829	XP_963786.1

Gene Ontology
Bibliography

Related articles in PubMed

A mouse model of a human congenital disorder of glycosylation caused by loss of PMM2.
Chan B, Clasquin M, Smolen GA, Histen G, Powe J, Chen Y, Lin Z, Lu C, Liu Y, Cang Y, Yan Z, Xia Y, Thompson R, Singleton C, Dorsch M, Silverman L, Su SM, Freeze HH, Jin S
Human molecular genetics25(11)2182-2193(2016 06)

Toxic gain of function from mutant FUS protein is crucial to trigger cell autonomous motor neuron loss.
Scekic-Zahirovic J, Sendscheid O, El Oussini H, Jambeau M, Sun Y, Mersmann S, Wagner M, Dieterl? S, Sinniger J, Dirrig-Grosch S, Drenner K, Birling MC, Qiu J, Zhou Y, Li H, Fu XD, Rouaux C, Shelkovnikova T, Witting A, Ludolph AC, Kiefer F, Storkebaum E, Lagier-Tourenne C, Dupuis L
The EMBO journal35(10)1077-97(2016 05)

The tissue-specific lncRNA Fendrr is an essential regulator of heart and body wall development in the mouse.
Grote P, Wittler L, Hendrix D, Koch F, W?hrisch S, Beisaw A, Macura K, Bl?ss G, Kellis M, Werber M, Herrmann BG
Developmental cell24(2)206-14(2013 Jan)

Successful prenatal mannose treatment for congenital disorder of glycosylation-Ia in mice.
Schneider A, Thiel C, Rindermann J, DeRossi C, Popovici D, Hoffmann GF, Gr?ne HJ, K?rner C
Nature medicine18(1)71-3(2011 Dec)

Ontogeny of D-mannose transport and metabolism in rat small intestine.
Cano M, Ilundain AA
The Journal of membrane biology235(2)101-8(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This functional mouse model of PMM2-CDG, in vitro assays and identification of the novel gp130 biomarker all shed light on the human disease, and moreover, provide the essential tools to test potential therapeutics for this untreatable disease.
Title: A mouse model of a human congenital disorder of glycosylation caused by loss of PMM2.

Pmm2 activity in jejunum increased during the early stages of life, and it decreased at 1 month old, as does the amount of mannose incorporated into glycoproteins, whereas in the ileum, they were not affected by age.
Title: Ontogeny of D-mannose transport and metabolism in rat small intestine.

These results indicate that Pmm2 is essential for early development of mice.
Title: Targeted disruption of the mouse phosphomannomutase 2 gene causes early embryonic lethality.

Pmm2 immunoreactivity seen in prenatal brain until postnatal and adult stage and restricted in neuronal cell bodies.
Title: Tissue distribution of the murine phosphomannomutases Pmm1 and Pmm2 during brain development.

The following Pmm2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pmm2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15896	NM_016881.3 Latest version!	Mus musculus phosphomannomutase 2 (Pmm2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu84014	NM_001362485.1 Latest version!	Mus musculus phosphomannomutase 2 (Pmm2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu52276	XM_006522360.4 Latest version!	Mus musculus phosphomannomutase 2 (Pmm2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu52276	XM_006522360.3	Mus musculus phosphomannomutase 2 (Pmm2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu15896	NM_016881.2	Mus musculus phosphomannomutase 2 (Pmm2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pmm2 ( XM_006522360.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522423.1 Mus musculus phosphomannomutase 2 (Pmm2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu52276
Clone ID Related Accession (Same CDS sequence)	XM_006522360.3 , XM_006522360.4
Accession Version	XM_006522360.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phosphomannomutase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATA TTCAAGGGCA TCTGGGGGAG GATGTGATCC AAGACCTGAT CAACTACTGT 
CTGAGCTACA TTGCAAACAT TAAACTCCCT AAGAAAAGGG GAACTTTCAT TGAATTCCGA 
AATGGCATGT TGAATGTGTC CCCAATTGGA AGAAGCTGCA GCCAAGAAGA ACGAATTGAA 
TTCTACGAAC TCGATAAAAA AGAACATATA CGACAAAAGT TCGTAGCAGA CCTGCGGAAG 
GAGTTTGCAG GGAAAGGCCT CACGTTCTCC ATAGGTGGCC AAATCAGCAT TGACGTCTTT 
CCTGAAGGAT GGGATAAGCG GTATTGCCTG AGACACCTGG AACATGCTGG CTATAAGACC 
ATTTATTTCT TTGGAGACAA GACTATGCCG GGTGGCAATG ACCATGAGAT CTTCACAGAC 
CCCAGAACTG TGGGCTACAC AGTGACGGCC CCCGAAGACA CACGCAGGAT CTGTGAGGGG 
CTCTTCCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522423.1
CDS	175..666
Translation	MMNIQGHLGEDVIQDLINYCLSYIANIKLPKKRGTFIEFRNGMLNVSPIGRSCSQEERIEFYELDKKEHIRQKFVADLRKEFAGKGLTFSIGGQISIDVFPEGWDKRYCLRHLEHAGYKTIYFFGDKTMPGGNDHEIFTDPRTVGYTVTAPEDTRRICEGLFP

Target ORF information:

RefSeq Version	XM_006522360.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphomannomutase 2 (Pmm2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522360.3

ORF Insert Sequence:

ATGATGAATA TTCAAGGGCA TCTGGGGGAG GATGTGATCC AAGACCTGAT CAACTACTGT 
CTGAGCTACA TTGCAAACAT TAAACTCCCT AAGAAAAGGG GAACTTTCAT TGAATTCCGA 
AATGGCATGT TGAATGTGTC CCCAATTGGA AGAAGCTGCA GCCAAGAAGA ACGAATTGAA 
TTCTACGAAC TCGATAAAAA AGAACATATA CGACAAAAGT TCGTAGCAGA CCTGCGGAAG 
GAGTTTGCAG GGAAAGGCCT CACGTTCTCC ATAGGTGGCC AAATCAGCAT TGACGTCTTT 
CCTGAAGGAT GGGATAAGCG GTATTGCCTG AGACACCTGG AACATGCTGG CTATAAGACC 
ATTTATTTCT TTGGAGACAA GACTATGCCG GGTGGCAATG ACCATGAGAT CTTCACAGAC 
CCCAGAACTG TGGGCTACAC AGTGACGGCC CCCGAAGACA CACGCAGGAT CTGTGAGGGG 
CTCTTCCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pmm2 ( NM_016881.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058577.1 Mus musculus phosphomannomutase 2 (Pmm2), mRNA.

Price & Availability↑

CloneID	OMu15896
Clone ID Related Accession (Same CDS sequence)	NM_016881.2 , NM_016881.3
Accession Version	NM_016881.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Mus musculus(house mouse)
Product	phosphomannomutase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC115005.11. On Mar 21, 2007 this sequence version replaced NM_016881.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF043514.1, AK005074.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACTC TCTGTCTCTT CGACATGGAT GGGACCCTGA CTGCCCCGCG GCAGAAAATT 
ACAGAAGAAA TGGATGGCTT TCTACAAAAA TTGAGGCAGA AGACCAAAAT TGGAGTGGTA 
GGTGGGTCAG ATTTTGAGAA ACTGCAAGAG CAACTGGGAA ATGATGTGGT TGAGAAATAC 
GATTATGTGT TTCCAGAGAA TGGCTTGGTA GCGTACAAAG ATGGGAAGCT ATTGTGTAAG 
CAGAATATTC AAGGGCATCT GGGGGAGGAT GTGATCCAAG ACCTGATCAA CTACTGTCTG 
AGCTACATTG CAAACATTAA ACTCCCTAAG AAAAGGGGAA CTTTCATTGA ATTCCGAAAT 
GGCATGTTGA ATGTGTCCCC AATTGGAAGA AGCTGCAGCC AAGAAGAACG AATTGAATTC 
TACGAACTCG ATAAAAAAGA ACATATACGA CAAAAGTTCG TAGCAGACCT GCGGAAGGAG 
TTTGCAGGGA AAGGCCTCAC GTTCTCCATA GGTGGCCAAA TCAGCATTGA CGTCTTTCCT 
GAAGGATGGG ATAAGCGGTA TTGCCTGAGA CACCTGGAAC ATGCTGGCTA TAAGACCATT 
TATTTCTTTG GAGACAAGAC TATGCCGGGT GGCAATGACC ATGAGATCTT CACAGACCCC 
AGAACTGTGG GCTACACAGT GACGGCCCCC GAAGACACAC GCAGGATCTG TGAGGGGCTC 
TTCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058577.1
CDS	69..797
Translation	MATLCLFDMDGTLTAPRQKITEEMDGFLQKLRQKTKIGVVGGSDFEKLQEQLGNDVVEKYDYVFPENGLVAYKDGKLLCKQNIQGHLGEDVIQDLINYCLSYIANIKLPKKRGTFIEFRNGMLNVSPIGRSCSQEERIEFYELDKKEHIRQKFVADLRKEFAGKGLTFSIGGQISIDVFPEGWDKRYCLRHLEHAGYKTIYFFGDKTMPGGNDHEIFTDPRTVGYTVTAPEDTRRICEGLFP

Target ORF information:

RefSeq Version	NM_016881.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphomannomutase 2 (Pmm2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016881.2

ORF Insert Sequence:

ATGGCCACTC TCTGTCTCTT CGACATGGAT GGGACCCTGA CTGCCCCGCG GCAGAAAATT 
ACAGAAGAAA TGGATGGCTT TCTACAAAAA TTGAGGCAGA AGACCAAAAT TGGAGTGGTA 
GGTGGGTCAG ATTTTGAGAA ACTGCAAGAG CAACTGGGAA ATGATGTGGT TGAGAAATAC 
GATTATGTGT TTCCAGAGAA TGGCTTGGTA GCGTACAAAG ATGGGAAGCT ATTGTGTAAG 
CAGAATATTC AAGGGCATCT GGGGGAGGAT GTGATCCAAG ACCTGATCAA CTACTGTCTG 
AGCTACATTG CAAACATTAA ACTCCCTAAG AAAAGGGGAA CTTTCATTGA ATTCCGAAAT 
GGCATGTTGA ATGTGTCCCC AATTGGAAGA AGCTGCAGCC AAGAAGAACG AATTGAATTC 
TACGAACTCG ATAAAAAAGA ACATATACGA CAAAAGTTCG TAGCAGACCT GCGGAAGGAG 
TTTGCAGGGA AAGGCCTCAC GTTCTCCATA GGTGGCCAAA TCAGCATTGA CGTCTTTCCT 
GAAGGATGGG ATAAGCGGTA TTGCCTGAGA CACCTGGAAC ATGCTGGCTA TAAGACCATT 
TATTTCTTTG GAGACAAGAC TATGCCGGGT GGCAATGACC ATGAGATCTT CACAGACCCC 
AGAACTGTGG GCTACACAGT GACGGCCCCC GAAGACACAC GCAGGATCTG TGAGGGGCTC 
TTCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pmm2 ( NM_016881.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058577.1 Mus musculus phosphomannomutase 2 (Pmm2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15896
Clone ID Related Accession (Same CDS sequence)	NM_016881.2 , NM_016881.3
Accession Version	NM_016881.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	phosphomannomutase 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115005.11. On Apr 18, 2018 this sequence version replaced NM_016881.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK145402.1, AK005074.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACTC TCTGTCTCTT CGACATGGAT GGGACCCTGA CTGCCCCGCG GCAGAAAATT 
ACAGAAGAAA TGGATGGCTT TCTACAAAAA TTGAGGCAGA AGACCAAAAT TGGAGTGGTA 
GGTGGGTCAG ATTTTGAGAA ACTGCAAGAG CAACTGGGAA ATGATGTGGT TGAGAAATAC 
GATTATGTGT TTCCAGAGAA TGGCTTGGTA GCGTACAAAG ATGGGAAGCT ATTGTGTAAG 
CAGAATATTC AAGGGCATCT GGGGGAGGAT GTGATCCAAG ACCTGATCAA CTACTGTCTG 
AGCTACATTG CAAACATTAA ACTCCCTAAG AAAAGGGGAA CTTTCATTGA ATTCCGAAAT 
GGCATGTTGA ATGTGTCCCC AATTGGAAGA AGCTGCAGCC AAGAAGAACG AATTGAATTC 
TACGAACTCG ATAAAAAAGA ACATATACGA CAAAAGTTCG TAGCAGACCT GCGGAAGGAG 
TTTGCAGGGA AAGGCCTCAC GTTCTCCATA GGTGGCCAAA TCAGCATTGA CGTCTTTCCT 
GAAGGATGGG ATAAGCGGTA TTGCCTGAGA CACCTGGAAC ATGCTGGCTA TAAGACCATT 
TATTTCTTTG GAGACAAGAC TATGCCGGGT GGCAATGACC ATGAGATCTT CACAGACCCC 
AGAACTGTGG GCTACACAGT GACGGCCCCC GAAGACACAC GCAGGATCTG TGAGGGGCTC 
TTCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058577.1
CDS	69..797
Translation	MATLCLFDMDGTLTAPRQKITEEMDGFLQKLRQKTKIGVVGGSDFEKLQEQLGNDVVEKYDYVFPENGLVAYKDGKLLCKQNIQGHLGEDVIQDLINYCLSYIANIKLPKKRGTFIEFRNGMLNVSPIGRSCSQEERIEFYELDKKEHIRQKFVADLRKEFAGKGLTFSIGGQISIDVFPEGWDKRYCLRHLEHAGYKTIYFFGDKTMPGGNDHEIFTDPRTVGYTVTAPEDTRRICEGLFP

Target ORF information:

RefSeq Version	NM_016881.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphomannomutase 2 (Pmm2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016881.3

ORF Insert Sequence:

ATGGCCACTC TCTGTCTCTT CGACATGGAT GGGACCCTGA CTGCCCCGCG GCAGAAAATT 
ACAGAAGAAA TGGATGGCTT TCTACAAAAA TTGAGGCAGA AGACCAAAAT TGGAGTGGTA 
GGTGGGTCAG ATTTTGAGAA ACTGCAAGAG CAACTGGGAA ATGATGTGGT TGAGAAATAC 
GATTATGTGT TTCCAGAGAA TGGCTTGGTA GCGTACAAAG ATGGGAAGCT ATTGTGTAAG 
CAGAATATTC AAGGGCATCT GGGGGAGGAT GTGATCCAAG ACCTGATCAA CTACTGTCTG 
AGCTACATTG CAAACATTAA ACTCCCTAAG AAAAGGGGAA CTTTCATTGA ATTCCGAAAT 
GGCATGTTGA ATGTGTCCCC AATTGGAAGA AGCTGCAGCC AAGAAGAACG AATTGAATTC 
TACGAACTCG ATAAAAAAGA ACATATACGA CAAAAGTTCG TAGCAGACCT GCGGAAGGAG 
TTTGCAGGGA AAGGCCTCAC GTTCTCCATA GGTGGCCAAA TCAGCATTGA CGTCTTTCCT 
GAAGGATGGG ATAAGCGGTA TTGCCTGAGA CACCTGGAAC ATGCTGGCTA TAAGACCATT 
TATTTCTTTG GAGACAAGAC TATGCCGGGT GGCAATGACC ATGAGATCTT CACAGACCCC 
AGAACTGTGG GCTACACAGT GACGGCCCCC GAAGACACAC GCAGGATCTG TGAGGGGCTC 
TTCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pmm2 ( NM_001362485.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349414.1 Mus musculus phosphomannomutase 2 (Pmm2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu84014
Clone ID Related Accession (Same CDS sequence)	NM_001362485.1
Accession Version	NM_001362485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	phosphomannomutase 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF043514.1, AK002858.1 and AC115005.11. ##Evidence-Data-START## Transcript exon combination :: AK002858.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACTC TCTGTCTCTT CGACATGGAT GGGACCCTGA CTGCCCCGCG GCAGAAAATT 
ACAGAAGAAA TGGATGGCTT TCTACAAAAA TTGAGGCAGA AGACCAAAAT TGGAGTGGTA 
GGTGGGTCAG ATTTTGAGAA ACTGCAAGAG CAACTGGGAA ATGATGAGAA TGGCTTGGTA 
GCGTACAAAG ATGGGAAGCT ATTGTGTAAG CAGAATATTC AAGGGCATCT GGGGGAGGAT 
GTGATCCAAG ACCTGATCAA CTACTGTCTG AGCTACATTG CAAACATTAA ACTCCCTAAG 
AAAAGGGGAA CTTTCATTGA ATTCCGAAAT GGCATGTTGA ATGTGTCCCC AATTGGAAGA 
AGCTGCAGCC AAGAAGAACG AATTGAATTC TACGAACTCG ATAAAAAAGA ACATATACGA 
CAAAAGTTCG TAGCAGACCT GCGGAAGGAG TTTGCAGGGA AAGGCCTCAC GTTCTCCATA 
GGTGGCCAAA TCAGCATTGA CGTCTTTCCT GAAGGATGGG ATAAGCGGTA TTGCCTGAGA 
CACCTGGAAC ATGCTGGCTA TAAGACCATT TATTTCTTTG GAGACAAGAC TATGCCGGGT 
GGCAATGACC ATGAGATCTT CACAGACCCC AGAACTGTGG GCTACACAGT GACGGCCCCC 
GAAGACACAC GCAGGATCTG TGAGGGGCTC TTCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349414.1
CDS	69..767
Translation	MATLCLFDMDGTLTAPRQKITEEMDGFLQKLRQKTKIGVVGGSDFEKLQEQLGNDENGLVAYKDGKLLCKQNIQGHLGEDVIQDLINYCLSYIANIKLPKKRGTFIEFRNGMLNVSPIGRSCSQEERIEFYELDKKEHIRQKFVADLRKEFAGKGLTFSIGGQISIDVFPEGWDKRYCLRHLEHAGYKTIYFFGDKTMPGGNDHEIFTDPRTVGYTVTAPEDTRRICEGLFP

Target ORF information:

RefSeq Version	NM_001362485.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphomannomutase 2 (Pmm2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362485.1

ORF Insert Sequence:

ATGGCCACTC TCTGTCTCTT CGACATGGAT GGGACCCTGA CTGCCCCGCG GCAGAAAATT 
ACAGAAGAAA TGGATGGCTT TCTACAAAAA TTGAGGCAGA AGACCAAAAT TGGAGTGGTA 
GGTGGGTCAG ATTTTGAGAA ACTGCAAGAG CAACTGGGAA ATGATGAGAA TGGCTTGGTA 
GCGTACAAAG ATGGGAAGCT ATTGTGTAAG CAGAATATTC AAGGGCATCT GGGGGAGGAT 
GTGATCCAAG ACCTGATCAA CTACTGTCTG AGCTACATTG CAAACATTAA ACTCCCTAAG 
AAAAGGGGAA CTTTCATTGA ATTCCGAAAT GGCATGTTGA ATGTGTCCCC AATTGGAAGA 
AGCTGCAGCC AAGAAGAACG AATTGAATTC TACGAACTCG ATAAAAAAGA ACATATACGA 
CAAAAGTTCG TAGCAGACCT GCGGAAGGAG TTTGCAGGGA AAGGCCTCAC GTTCTCCATA 
GGTGGCCAAA TCAGCATTGA CGTCTTTCCT GAAGGATGGG ATAAGCGGTA TTGCCTGAGA 
CACCTGGAAC ATGCTGGCTA TAAGACCATT TATTTCTTTG GAGACAAGAC TATGCCGGGT 
GGCAATGACC ATGAGATCTT CACAGACCCC AGAACTGTGG GCTACACAGT GACGGCCCCC 
GAAGACACAC GCAGGATCTG TGAGGGGCTC TTCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pmm2 ( XM_006522360.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522423.1 Mus musculus phosphomannomutase 2 (Pmm2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu52276
Clone ID Related Accession (Same CDS sequence)	XM_006522360.3 , XM_006522360.4
Accession Version	XM_006522360.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphomannomutase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522360.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATA TTCAAGGGCA TCTGGGGGAG GATGTGATCC AAGACCTGAT CAACTACTGT 
CTGAGCTACA TTGCAAACAT TAAACTCCCT AAGAAAAGGG GAACTTTCAT TGAATTCCGA 
AATGGCATGT TGAATGTGTC CCCAATTGGA AGAAGCTGCA GCCAAGAAGA ACGAATTGAA 
TTCTACGAAC TCGATAAAAA AGAACATATA CGACAAAAGT TCGTAGCAGA CCTGCGGAAG 
GAGTTTGCAG GGAAAGGCCT CACGTTCTCC ATAGGTGGCC AAATCAGCAT TGACGTCTTT 
CCTGAAGGAT GGGATAAGCG GTATTGCCTG AGACACCTGG AACATGCTGG CTATAAGACC 
ATTTATTTCT TTGGAGACAA GACTATGCCG GGTGGCAATG ACCATGAGAT CTTCACAGAC 
CCCAGAACTG TGGGCTACAC AGTGACGGCC CCCGAAGACA CACGCAGGAT CTGTGAGGGG 
CTCTTCCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522423.1
CDS	323..814
Translation	MMNIQGHLGEDVIQDLINYCLSYIANIKLPKKRGTFIEFRNGMLNVSPIGRSCSQEERIEFYELDKKEHIRQKFVADLRKEFAGKGLTFSIGGQISIDVFPEGWDKRYCLRHLEHAGYKTIYFFGDKTMPGGNDHEIFTDPRTVGYTVTAPEDTRRICEGLFP

Target ORF information:

RefSeq Version	XM_006522360.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphomannomutase 2 (Pmm2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522360.4

ORF Insert Sequence:

ATGATGAATA TTCAAGGGCA TCTGGGGGAG GATGTGATCC AAGACCTGAT CAACTACTGT 
CTGAGCTACA TTGCAAACAT TAAACTCCCT AAGAAAAGGG GAACTTTCAT TGAATTCCGA 
AATGGCATGT TGAATGTGTC CCCAATTGGA AGAAGCTGCA GCCAAGAAGA ACGAATTGAA 
TTCTACGAAC TCGATAAAAA AGAACATATA CGACAAAAGT TCGTAGCAGA CCTGCGGAAG 
GAGTTTGCAG GGAAAGGCCT CACGTTCTCC ATAGGTGGCC AAATCAGCAT TGACGTCTTT 
CCTGAAGGAT GGGATAAGCG GTATTGCCTG AGACACCTGG AACATGCTGG CTATAAGACC 
ATTTATTTCT TTGGAGACAA GACTATGCCG GGTGGCAATG ACCATGAGAT CTTCACAGAC 
CCCAGAACTG TGGGCTACAC AGTGACGGCC CCCGAAGACA CACGCAGGAT CTGTGAGGGG 
CTCTTCCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A mouse model of a human congenital disorder of glycosylation caused by loss of PMM2.
Human molecular genetics25(11)2182-2193(2016 06)
Chan B,Clasquin M,Smolen GA,Histen G,Powe J,Chen Y,Lin Z,Lu C,Liu Y,Cang Y,Yan Z,Xia Y,Thompson R,Singleton C,Dorsch M,Silverman L,Su SM,Freeze HH,Jin S

Toxic gain of function from mutant FUS protein is crucial to trigger cell autonomous motor neuron loss.
The EMBO journal35(10)1077-97(2016 05)
Scekic-Zahirovic J,Sendscheid O,El Oussini H,Jambeau M,Sun Y,Mersmann S,Wagner M,Dieterl? S,Sinniger J,Dirrig-Grosch S,Drenner K,Birling MC,Qiu J,Zhou Y,Li H,Fu XD,Rouaux C,Shelkovnikova T,Witting A,Ludolph AC,Kiefer F,Storkebaum E,Lagier-Tourenne C,Dupuis L

The tissue-specific lncRNA Fendrr is an essential regulator of heart and body wall development in the mouse.
Developmental cell24(2)206-14(2013 Jan)
Grote P,Wittler L,Hendrix D,Koch F,W?hrisch S,Beisaw A,Macura K,Bl?ss G,Kellis M,Werber M,Herrmann BG

Successful prenatal mannose treatment for congenital disorder of glycosylation-Ia in mice.
Nature medicine18(1)71-3(2011 Dec)
Schneider A,Thiel C,Rindermann J,DeRossi C,Popovici D,Hoffmann GF,Gr?ne HJ,K?rner C

Ontogeny of D-mannose transport and metabolism in rat small intestine.
The Journal of membrane biology235(2)101-8(2010 Jun)
Cano M,Ilundain AA

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Pmm2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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