Clpp cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clpp
Entrez Gene ID	53895
Full Name	caseinolytic mitochondrial matrix peptidase proteolytic subunit
Synonyms	AU019820,D17Wsu160e
General protein information	Preferred Names caseinolytic mitochondrial matrix peptidase proteolytic subunit Names ATP-dependent Clp protease proteolytic subunit, mitochondrial ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog caseinolytic protease, ATP-dependent, proteolytic subunit homolog endopeptidase Clp putative ATP-dependent Clp protease proteolytic subunit, mitochondrial
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 D\|17 29.61 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_017393.2	NP_059089.1	ATP-dependent Clp protease proteolytic subunit, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CLPP	NP_006003.1
Arabidopsis thaliana (thale cress)	NCLPP7	NP_568427.1
Canis lupus familiaris (dog)	CLPP	XP_854538.1
Bos taurus (cattle)	CLPP	NP_001039879.1
Rattus norvegicus (Norway rat)	Clpp	XP_217313.6
Caenorhabditis elegans (roundworm)	clpp-1	NP_001254239.1
Oryza sativa (rice)	Os04g0525600	NP_001053358.1
Oryza sativa (rice)	Os02g0634500	NP_001047512.1
Drosophila melanogaster (fruit fly)	CG5045	NP_001260332.1
Pan troglodytes (chimpanzee)	CLPP	XP_512312.2
Mus musculus (house mouse)	Clpp	NP_059089.1
Xenopus tropicalis (tropical clawed frog)	clpp	XP_002940664.2
Danio rerio (zebrafish)	clpp	NP_001018520.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009019	XP_319765.3

Gene Ontology
Bibliography

Related articles in PubMed

CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.
Szczepanowska K, Maiti P, Kukat A, Hofsetz E, Nolte H, Senft K, Becker C, Ruzzenente B, Hornig-Do HT, Wibom R, Wiesner RJ, Kr?ger M, Trifunovic A
The EMBO journal35(23)2566-2583(2016 12)

Frataxin deficiency causes upregulation of mitochondrial Lon and ClpP proteases and severe loss of mitochondrial Fe-S proteins.
Guillon B, Bulteau AL, Wattenhofer-Donz? M, Schmucker S, Friguet B, Puccio H, Drapier JC, Bouton C
The FEBS journal276(4)1036-47(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors present evidence that mammalian CLPP has an essential role in determining the rate of mitochondrial protein synthesis by regulating the level of mitoribosome assembly.
Title: CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.

we demonstrate that a strong mitochondrial cardiomyopathy and diminished respiration due to DARS2 deficiency can be alleviated by the loss of CLPP, leading to an increased de novo synthesis of individual OXPHOS subunits.
Title: Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt.

Novel effects of ClpP on mitochondrial morphology, cell proliferation and protein translation pathways in muscle cells.
Title: Down-regulation of the mitochondrial matrix peptidase ClpP in muscle cells causes mitochondrial dysfunction and decreases cell proliferation.

Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors.
Title: Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors.

The correlative effect of Lon and ClpP upregulation on loss of mitochondrial Fe-S proteins during the progression of the disease may suggest that Fe-S proteins are potential targets of Lon and ClpP proteases in FRDA.
Title: Frataxin deficiency causes upregulation of mitochondrial Lon and ClpP proteases and severe loss of mitochondrial Fe-S proteins.

The following Clpp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clpp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu23375	NM_017393.2 Latest version!	Mus musculus caseinolytic mitochondrial matrix peptidase proteolytic subunit (Clpp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clpp ( NM_017393.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_059089.1 Mus musculus caseinolytic mitochondrial matrix peptidase proteolytic subunit (Clpp), mRNA.

Price & Availability↑

CloneID	OMu23375
Clone ID Related Accession (Same CDS sequence)	NM_017393.2
Accession Version	NM_017393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	ATP-dependent Clp protease proteolytic subunit, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY294002.1, AK145765.1 and AC073683.3. On Aug 14, 2009 this sequence version replaced NM_017393.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK145765.1, BC001998.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGCCCA GAGTGCTGCT GGGGGAGGCC CGGGTGGCTG TGGACGGATG TCGCGCTCTG 
TTGTCTCGCC TTGCCGTGCA TTTCTCCCCG CCATGGACTG CTGTGAGCTG CTCACCCCTG 
CGGAGGAGCC TGCATGGAAC TGCGACGCGA GCTTTCCCGC TCATCCCCAT AGTGGTGGAG 
CAGACGGGTC GAGGCGAGCG CGCTTATGAC ATATACTCGA GGCTGTTGCG GGAACGCATC 
GTGTGCGTCA TGGGCCCGAT TGACGACAGT GTGGCCAGTC TGGTCATTGC CCAGCTGTTG 
TTCTTACAGT CTGAAAGCAA CAAGAAGCCC ATTCATATGT ATATCAACAG CCCAGGTGGT 
GTGGTAACTG CGGGCCTGGC CATCTACGAC ACAATGCAGT ACATCCTGAA CCCCATCTGC 
ACGTGGTGTG TTGGACAGGC TGCCAGCATG GGCTCCCTGC TCCTCGCTGC TGGCAGCCCG 
GGCATGCGCC ATTCACTGCC CAATTCCAGA ATCATGATCC ACCAGCCCTC TGGAGGAGCC 
AGGGGCCAAG CCACAGACAT CGCCATCCAG GCAGAGGAAA TCATGAAGCT GAAAAAGCAG 
CTATACAACA TCTACGCCAA ACACACCAAG CAGAGCCTAC AGGTGATCGA GTCAGCAATG 
GAGAGGGACC GCTACATGAG CCCCATGGAG GCCCAAGAGT TTGGCATCTT GGACAAGGTC 
TTGGTCCACC CACCTCAGGA CGGGGAGGAT GAGCCAGAAC TGGTACAGAA GGAGACTGCC 
ACAGCGCCGA CGGATCCTCC TGCCCCGACA AGCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059089.1
CDS	65..883
Translation	MWPRVLLGEARVAVDGCRALLSRLAVHFSPPWTAVSCSPLRRSLHGTATRAFPLIPIVVEQTGRGERAYDIYSRLLRERIVCVMGPIDDSVASLVIAQLLFLQSESNKKPIHMYINSPGGVVTAGLAIYDTMQYILNPICTWCVGQAASMGSLLLAAGSPGMRHSLPNSRIMIHQPSGGARGQATDIAIQAEEIMKLKKQLYNIYAKHTKQSLQVIESAMERDRYMSPMEAQEFGILDKVLVHPPQDGEDEPELVQKETATAPTDPPAPTST

Target ORF information:

RefSeq Version	NM_017393.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus caseinolytic mitochondrial matrix peptidase proteolytic subunit (Clpp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017393.2

ORF Insert Sequence:

ATGTGGCCCA GAGTGCTGCT GGGGGAGGCC CGGGTGGCTG TGGACGGATG TCGCGCTCTG 
TTGTCTCGCC TTGCCGTGCA TTTCTCCCCG CCATGGACTG CTGTGAGCTG CTCACCCCTG 
CGGAGGAGCC TGCATGGAAC TGCGACGCGA GCTTTCCCGC TCATCCCCAT AGTGGTGGAG 
CAGACGGGTC GAGGCGAGCG CGCTTATGAC ATATACTCGA GGCTGTTGCG GGAACGCATC 
GTGTGCGTCA TGGGCCCGAT TGACGACAGT GTGGCCAGTC TGGTCATTGC CCAGCTGTTG 
TTCTTACAGT CTGAAAGCAA CAAGAAGCCC ATTCATATGT ATATCAACAG CCCAGGTGGT 
GTGGTAACTG CGGGCCTGGC CATCTACGAC ACAATGCAGT ACATCCTGAA CCCCATCTGC 
ACGTGGTGTG TTGGACAGGC TGCCAGCATG GGCTCCCTGC TCCTCGCTGC TGGCAGCCCG 
GGCATGCGCC ATTCACTGCC CAATTCCAGA ATCATGATCC ACCAGCCCTC TGGAGGAGCC 
AGGGGCCAAG CCACAGACAT CGCCATCCAG GCAGAGGAAA TCATGAAGCT GAAAAAGCAG 
CTATACAACA TCTACGCCAA ACACACCAAG CAGAGCCTAC AGGTGATCGA GTCAGCAATG 
GAGAGGGACC GCTACATGAG CCCCATGGAG GCCCAAGAGT TTGGCATCTT GGACAAGGTC 
TTGGTCCACC CACCTCAGGA CGGGGAGGAT GAGCCAGAAC TGGTACAGAA GGAGACTGCC 
ACAGCGCCGA CGGATCCTCC TGCCCCGACA AGCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.
The EMBO journal35(23)2566-2583(2016 12)
Szczepanowska K,Maiti P,Kukat A,Hofsetz E,Nolte H,Senft K,Becker C,Ruzzenente B,Hornig-Do HT,Wibom R,Wiesner RJ,Kr?ger M,Trifunovic A

Frataxin deficiency causes upregulation of mitochondrial Lon and ClpP proteases and severe loss of mitochondrial Fe-S proteins.
The FEBS journal276(4)1036-47(2009 Feb)
Guillon B,Bulteau AL,Wattenhofer-Donz? M,Schmucker S,Friguet B,Puccio H,Drapier JC,Bouton C

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Clpp cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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