Prrc2a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prrc2a
Entrez Gene ID	53761
Full Name	proline-rich coiled-coil 2A
Synonyms	3110039B05Rik,Bat-2,Bat2,D17H6S51E,G2,Wbp12,mKIAA4178
General protein information	Preferred Names proline-rich coiled-coil 2A Names protein PRRC2A HLA-B associated transcript 2 large proline-rich protein BAT2 proline-rich and coiled-coil-containing protein 2A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 B1\|17 18.59 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006524620.3	XP_006524683.1	protein PRRC2A isoform X1
XM_006524621.3	XP_006524684.1	protein PRRC2A isoform X2
NM_001199044.1	NP_001185973.1	protein PRRC2A isoform 2
XM_006524620.4	XP_006524683.1	protein PRRC2A isoform X1
XM_030249899.1	XP_030105759.1	protein PRRC2A isoform X2
XM_030249900.1	XP_030105760.1	protein PRRC2A isoform X3
NM_020027.3	NP_064411.2	protein PRRC2A isoform 1
XM_006524621.4	XP_006524684.1	protein PRRC2A isoform X3

Pathways from BioSystems

Homologies

	PRRC2A	NP_001108429.1
Xenopus tropicalis (tropical clawed frog)	prrc2a	XP_004916983.1
Bos taurus (cattle)	PRRC2A	NP_001192486.1
Homo sapiens (human)	PRRC2A	NP_542417.2
Rattus norvegicus (Norway rat)	Prrc2a	NP_997627.1
Pan troglodytes (chimpanzee)	PRRC2A	XP_001156097.1
Canis lupus familiaris (dog)	PRRC2A	XP_005627193.1
Mus musculus (house mouse)	Prrc2a	NP_001185973.1

Gene Ontology
Bibliography

Related articles in PubMed

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.
Munton RP, Tweedie-Cullen R, Livingstone-Zatchej M, Weinandy F, Waidelich M, Longo D, Gehrig P, Potthast F, Rutishauser D, Gerrits B, Panse C, Schlapbach R, Mansuy IM
Molecular & cellular proteomics : MCP6(2)283-93(2007 Feb)

Comprehensive identification of phosphorylation sites in postsynaptic density preparations.
Trinidad JC, Specht CG, Thalhammer A, Schoepfer R, Burlingame AL
Molecular & cellular proteomics : MCP5(5)914-22(2006 May)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse.
Xie T, Rowen L, Aguado B, Ahearn ME, Madan A, Qin S, Campbell RD, Hood L
Genome research13(12)2621-36(2003 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prrc2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prrc2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15568	XM_006524620.4 Latest version!	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu15766	XM_030249899.1 Latest version!	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu53832	XM_006524621.4 Latest version!	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu53832	XM_030249900.1 Latest version!	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu15568	NM_020027.3 Latest version!	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu15766	NM_001199044.1 Latest version!	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu15568	XM_006524620.3	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu53832	XM_006524621.3	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prrc2a ( XM_006524620.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524683.1 Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15568
Clone ID Related Accession (Same CDS sequence)	XM_006524620.3 , XM_006524620.4 , NM_020027.3
Accession Version	XM_006524620.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein PRRC2A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524620.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGGCAAGTCC TTAGAGATCC AGAAACCCGC TGTTGCCCCT 
CGACATGGCC TGCAGAGTCT CGGAAAAGTT GCCATTGCCC GGCGCATGCC ACCCCCAGCC 
AACCTTCCAA GCCTGAAAGC CGAGAACAAA GGCAATGACC CCAATGTCTC ACTAGTGCCG 
AAAGACGGAA CAGGATGGGC AAGCAAACAG GAGCAGTCCG ACCCCAAGAG TTCCGATGCC 
TCAACCGCTC AGCCGCCGGA ATCGCAGCCA CTGCCGGCTT CACAGACGCC TGCCTCCAAC 
CAGCCGAAAC GACCCCCAAC AGCCCCCGAG AACACTCCTT CGGTTCCAAG CGGGGTAAAG 
TCCTGGGCAC AAGCCAGTGT CACCCATGGA GCCCATGGAG ATGGTGGAAG GGCATCAAAC 
CTACTGTCGC GATTCTCTCG AGAGGAATTT CCGACCCTGC AGGCGGCTGG AGACCAGGAC 
AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA CAGTCGTCTG GGCCCGGACC AAGCCTCCGC 
CCCCAAAATT CTACAACTTG GAGGGACGGA GGTGGGCGTG GCCCTGATGA CCTGGAGGGC 
CCGGACTCCA AACTTCATCA TGGCCATGAT CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA 
CCCCAGTTCC CTCCCTACCG CGGGATGATG CCGCCCTTTA TGTATCCCCC ATATCTCCCA 
TTCCCTCCGC CCTATGGACC CCAGGGGCCT TATAGATACC CGACTCCTGA TGGGCCCAGC 
CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC TCAGGGCCAC CAATGCGCCT TGTAGAGCCT 
GTGGGTCGGC CTTCTATTCT TAAAGAGGAT AATCTTAAAG AGTTTGACCA ACTGGACCAG 
GAAAATGATG ATGGTTGGGC AGGGGCCCAT GAAGAGGTTG ACTATACTGA AAAGCTCAAA 
TTCAGCGATG AGGAAGATGG ACGAGACTCC GACGAGGAGG GAGCTGAGGG CCACAAGGAT 
TCCCAGTCAG CTGCTGCTGA GGAACCTGAG ACAGATGGCA AAAAGGGCAC CTCCCCTGGC 
AGCGAATTGC CCCCTCCCAA GACAGCCTGG ACAGAGAATG CTCGGCCTTC AGAGACAGAG 
CCAGCTCCTC CTACCCCAAA GCCTCCCCCA CCTCCACCTC ACCGGGGGCC AGTCGGGAAC 
TGGGGGCCCC CTGGGGACTA CCCAGATCGA GGGGGTCCTC CCTGCAAACC CCCAGCACCT 
GAGGATGAGG ATGAGGCATG GCGGCAGCGT CGGAAGCAAT CTTCTTCAGA GATCTCCCTG 
GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA GAGGAAGAGC GACGCATGCA GGAGGAGCGC 
CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT CTTGATGAAA AGTTTGGGGC ACCTGACAAG 
CGGCTCAAGG CAGAGCCTGC AGCCCCACCT GTTACCCCTG CTGCCCCAGC TCTACCACCT 
GTAGTCCCCA AAGAAATTCC TGCAGCTCCA GCTCTGCCAC CAACACCTAC CCCAACACCA 
GAGAAAGAAC CTGAAGAGCC GGCACAAGCC CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG 
GTGGCTCCAG TTCCCACTCT GGTGAGTGGG GGTGGTTGCA CTGCCAACAG CAACAGCAGT 
GGCAGCTTCG AAGCCAGCCC AGTGGAACCC CAACTGCCTT CAAAAGAGGG TCCTGAACCA 
CCAGAAGAGG TTCCTCCACC TACCACACCC CCAGCCCCAA AGATGGAACC CAAAGGTGAT 
GGGGTTGGTT CTACTCGCCA GCCCCCCAGT CAGGGCTTGG GCTACCCCAA ATACCAGAAG 
TCATTGCCTC CTCGCTTCCA GCGGCAGCAA CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG 
CAACAGCAGT GGCAGCAGCA GCAACAAGGC ACTGCCCCTC CTGCCCCAGT GCCCCCATCA 
CCACCACAAC CTGTGACCCT GGGAGCTGTA CCTGCCCCAC AAGCACCGCC CCCACCTCCC 
AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG CCCCCACCAA TGCCTCCAAT GAACTTTGAC 
CCCCGCTGGA TGATGATTCC TCCTTACGTG GACCCTCGGC TTCTCCAGGG CCGGCCCCCT 
CTGGACTTCT ACCCTCCTGG TGTGCATCCC TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC 
AGTGGGGGCT CAAGCTCAGA GCCATTTGAA CGCCATGCAC CCCCTCTGTT GCGGGAAAGG 
GGCACCCCAC CAGTGGACCC CAAGTTGGCC TGGGTTGGAG ATGTCTTTAC CACCACACCC 
ACTGATCCTC GTCCTCTTAC CTCACCACTG CGCCAGGCAG CAGATGAGGA GGAGAAGAGT 
ATGAGGAGCG AGACTCCTCC GGTACCCCCG CCCCCACCTT ATCTGGCTAA TTATCCAGGT 
TTTCCAGAGA ATGGAACTCC TGGGCCCCCA ATCTCGAGAT TCCCTCTGGA GGAATCTGCT 
CCCCCGGGAC CCCGCCCACT ACCCTGGCCT CCAGGCAATG ATGAAGCTGC CAAGATGCAG 
GCTCCACCGC CCAAGAAGGA ACCCTCGAAG GAGGAACCGC CACAGCTGTC CGGGCCAGAA 
GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA GGCCCACCAC CACCACGGAG AGAGAATCGT 
ACTGAGACTC GCTGGGGCCC CCGACCAGGC AGTTGTCGCC GTGGAATCCC TCCAGAGGAG 
CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT ATAAAGAAGC CACCACCACC TGTGAAAGTA 
GAGGAGCTGC CTCCTAAGTC CCTAGAACAG GGGGATGAGA CCCCCAAGGT CCCAAAGCCA 
GATGCACTCA AGACAGCTAA GGGCAAGGTG GGCCCCAAAG AAACCCCACC AGGTGGAAAC 
CTGTCTCCTG CACCTAGGCT TCGGCGGGAC TATTCCTATG AAAGAGTAGG TCCTACATCT 
TGCCGTGGTC GGGGCAGAGG CGAGTATTTT GCCAGAGGAA GGGGTTTTCG GGGGACCTAT 
GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT GAATTCCGAA GTTACCGAGA GTTCCGAGGA 
GACGATGGGC GTGGGGGTGG TTCAGGCGGA ACAAACCATC CTTCTGCTCC CCGAGGCCGC 
ACTGCCAGTG AGACACGAAG TGAGGGTTCA GAATATGAAG AAATCCCCAA GAGACGCCGC 
CAGCGTGGCT CTGAAACAGG CAGTGAGACT CATGAGAGTG ACCTGGCCCC CTCTGACAAG 
GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT GGTCAGGTCC CTCTTGCCCC CCCACAACCA 
GGAGCTCCTC CATCACCAGC CCCAGCCCGA TTCTCCACTG CCCGAGGTGG TCGTGTCTTC 
ACTCCAAGAG GAGTACCATC ACGCCGTGGC CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC 
TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG GTTCCAAAGA AACCTCCAAC AGGTCCTTTG 
CCTCCAAGTA AAGAACCTTT GAAAGAGAAG CTGATCTCAG GGCCTCTGTC CCCCATGTCT 
CGAGCTGGCA ACATGGGTGT AGGCATGGAA GATGGGGAGC GACCCCGACG GAGGCGACAT 
GGCAGGGCTC AACAGCAGGA CAAACCACCT CGTTTCCGAA GGCTGAAACA AGAACGTGAA 
AACGCTGCCA GAGGAGCTGA TGGCAAGCCC CCTTCCCTAA CCCTTGCAGC CTCTACCCCT 
GGACCTGAGG AGACACTAAC AGCAGCCACA GTTCCCCCAC CCCCTCGCCG CACAGCTGCT 
AAGTCTCCTG ATCTGTCAAA CCAGAACTCA GACCAAGCCA ATGAGGAATG GGAGACTGCA 
TCAGAGAGCA GCGACTTTGC CAGTGAGCGC CGGGGTGACA AGGAGACTCC CCCAGCAGCA 
CTTATGACCT CTAAAGCTGT GGGGACTCCT GGGGCCAATG CAGGTGGGGC TGGACCAGGC 
ATTTCAGCCA TGTCTCGTGG AGACCTGAGC CAACGAGCCA AGGATTTGAG CAAGCGGAGC 
TTCTCAAGTC AGCGGCCAGG CATGGATCGT CAAAACCGGC GCCCAGGCAC TGGGGGCAAG 
ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT GGTGGTGCAG GCCCTGGGGG ACGGACCGGC 
CCAGGACGAG GGGATAAGAG GAGCTGGCCC TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG 
GAGCGACCTC CGGGACTTCC TCTGCCTCCG CCACCCCCCA GCAGTTCTGC TGTCTTCCGC 
CTGGACCAAG TTATCCATAG CAACCCTGCT GGAATCCAAC AGGCTCTGGC CCAACTTAGC 
AGCCGCCAAG GAAATGTAAC TGCACCAGGG GGGCACCCAA GGCCTAAGCC TGGGCCTCCC 
CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC CCAACCCGTT ATGACCCCCC AAGGGCCAGC 
AGCGCTATCA GTTCTGACCC CCACTTCGAG GAGCCAGGGC CGATGGTGAG GGGGGTGGGC 
GGGACTCCCC GGGACTCTGC CGGGGTTAAT CCCTTCCCCC CGAAACGGCG GGAGCGGCCT 
CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA ACTGTGCCAG CTTCTCATAG CTCTGGATTC 
TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT CAGGAAGAAT CCAGAGATTC AGGCACAGAG 
GCCCTAACTC CCCACATCTG GAATCGTTTA CATACTGCCA CAAGCCGAAA GAGTTACCAG 
CCTGGCTCCA TCGAGCCCTG GATGGAGCCC CTGAGTCCCT TTGAGGATGT GGCAGGCACA 
GAGATGAGTC AGTCTGACAG TGGAGTGGAC CTGAGTGGAG ATTCTCAGGT GTCATCAGGT 
CCCTGCAGTC AGCGAAGTTC CCCTGATGGA GGACTTAAGG GGTCAGCAGA GGGACCCCCC 
AAGCGGCCTG GAGGACCCTC ACCCCTGAAT GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT 
GAACCCTCTG AACCTCCTAG GAGACGGCCA CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG 
CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT CCCATAGGCA CAGAACGATC ACAGCGCACA 
GATCGAGGCC CAGAGCCAGG CCCTCTCCGA CCAGCCCACC GACCGGGCTC TCAAGTTGAG 
TTTGGCACTA CCAACAAGGA CTCAGACTTG TGCCTAGTGG TCGGAGACAC TTTAAAAGGA 
GAAAAGGAGT TGGTGGCATC AGCCACAGAG GCCGTTCCCA TATCCAGAGA CTGGGAGCTG 
CTCCCTAGTG CTTCTACTTC AGCTGAGCCA CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT 
GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT CCGTACCCTG AGGTCTTCTA TGGCAGCCCT 
GGACCTCCTA ATTCCCAGCA GGTCTCTGGG GGTGCACCCA TCGACTCTCA GTTACATCCC 
AACAGTGGAG GCTTCCGTCC TGGGACACCC TCATTGCACC AGTACAGGTC ACAGCCTCTG 
TATCTGCCCC CAGGTCCAGC CCCTCCCTCG GCACTGCTTT CTGGGGTAGC TCTCAAGGGC 
CAGTTTCTGG ATTTCTCAGC ACTGCAAGCC ACAGAGCTGG GGAAGTTGCC TGCTGGGGGA 
GTTCTCTACC CTCCGCCATC CTTCCTCTAC TCTGCAGCTT TCTGCCCCAG CCCTTTGCCT 
GACCCACCCT TGCTTCAGGT ACGCCAGGAT CTGCCATCCC CTTCAGATTT TTACTCTACT 
CCTCTGCAGC CTGGTGGCCA GAGTGGCTTC CTTCCTTCGG GAGCCCCTGC TCAGCAGATG 
CTTCTACCTG TGGTAGACTC ACAGCTGCCA GTGGTGAACT TTGGCTCCCT GCCACCAGCG 
CCACCCCCTG CTCCACCCCC CCTTTCTCTG TTACCTGTGG GTCCTGCTCT CCAGCCCCCC 
AACCTAGCTG TGCGGCCCCC ACCTGCTCCT GCTGCTCGGG TCTTACCTTC ACCCGCTAGA 
CCCTTCGCCC CTAGCTTGGG GCGAGCAGAG CTGCACCCAG TAGAACTAAA GCCTTTCCAG 
GATTACCGAA AGCTGAGCAG CAACCTTGGG GGTCCTGGGT CGTCACGGAC TCCCCCATCT 
GGAAGGCCCT TCTCTGGTCT CAATTCCCGT CTCAAGGCCC CACCTTCCAC CTACAGTGGA 
GTATTCCGAA CTCAGCGCAT TGACCTCTAC CAGCAGGCTT CCCCACCAGA TGCCCTGCGC 
TGGATGCCGA AGCCTTGGGA ACGGGCAGGG CCACCTTCTC GAGAAGGGCC TCCCCGAAGA 
GCAGAGGAGC CTGGGTCCCG GGGGGAGAAG GAGCCTGGAT TGCCCCCACC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524683.1
CDS	312..6788
Translation	MSDRSGPTAKGKDGKKYSSLNLFDTYKGKSLEIQKPAVAPRHGLQSLGKVAIARRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQSDPKSSDASTAQPPESQPLPASQTPASNQPKRPPTAPENTPSVPSGVKSWAQASVTHGAHGDGGRASNLLSRFSREEFPTLQAAGDQDKAAKERESAEQSSGPGPSLRPQNSTTWRDGGGRGPDDLEGPDSKLHHGHDPRGGLQPSGPPQFPPYRGMMPPFMYPPYLPFPPPYGPQGPYRYPTPDGPSRFPRVAGPRGSGPPMRLVEPVGRPSILKEDNLKEFDQLDQENDDGWAGAHEEVDYTEKLKFSDEEDGRDSDEEGAEGHKDSQSAAAEEPETDGKKGTSPGSELPPPKTAWTENARPSETEPAPPTPKPPPPPPHRGPVGNWGPPGDYPDRGGPPCKPPAPEDEDEAWRQRRKQSSSEISLAVERARRRREEEERRMQEERRAACAEKLKRLDEKFGAPDKRLKAEPAAPPVTPAAPALPPVVPKEIPAAPALPPTPTPTPEKEPEEPAQAPPVQAAPSPGVAPVPTLVSGGGCTANSNSSGSFEASPVEPQLPSKEGPEPPEEVPPPTTPPAPKMEPKGDGVGSTRQPPSQGLGYPKYQKSLPPRFQRQQQEQLLKQQQQQQQWQQQQQGTAPPAPVPPSPPQPVTLGAVPAPQAPPPPPKALYPGALGRPPPMPPMNFDPRWMMIPPYVDPRLLQGRPPLDFYPPGVHPSGLVPRERSDSGGSSSEPFERHAPPLLRERGTPPVDPKLAWVGDVFTTTPTDPRPLTSPLRQAADEEEKSMRSETPPVPPPPPYLANYPGFPENGTPGPPISRFPLEESAPPGPRPLPWPPGNDEAAKMQAPPPKKEPSKEEPPQLSGPEAGRKPARGGQGPPPPRRENRTETRWGPRPGSCRRGIPPEEPGVPPRRAGPIKKPPPPVKVEELPPKSLEQGDETPKVPKPDALKTAKGKVGPKETPPGGNLSPAPRLRRDYSYERVGPTSCRGRGRGEYFARGRGFRGTYGGRGRGARSREFRSYREFRGDDGRGGGSGGTNHPSAPRGRTASETRSEGSEYEEIPKRRRQRGSETGSETHESDLAPSDKEAPPPKEGVLGQVPLAPPQPGAPPSPAPARFSTARGGRVFTPRGVPSRRGRGGGRPPPVCSGWSPPAKSLVPKKPPTGPLPPSKEPLKEKLISGPLSPMSRAGNMGVGMEDGERPRRRRHGRAQQQDKPPRFRRLKQERENAARGADGKPPSLTLAASTPGPEETLTAATVPPPPRRTAAKSPDLSNQNSDQANEEWETASESSDFASERRGDKETPPAALMTSKAVGTPGANAGGAGPGISAMSRGDLSQRAKDLSKRSFSSQRPGMDRQNRRPGTGGKTGSGGGSSGGGGAGPGGRTGPGRGDKRSWPSPKNRSRPPEERPPGLPLPPPPPSSSAVFRLDQVIHSNPAGIQQALAQLSSRQGNVTAPGGHPRPKPGPPQAPQGSSPRPPTRYDPPRASSAISSDPHFEEPGPMVRGVGGTPRDSAGVNPFPPKRRERPPRKPELLQEETVPASHSSGFLGSKPEVPGPQEESRDSGTEALTPHIWNRLHTATSRKSYQPGSIEPWMEPLSPFEDVAGTEMSQSDSGVDLSGDSQVSSGPCSQRSSPDGGLKGSAEGPPKRPGGPSPLNAVPGESASGSEPSEPPRRRPPASHEGERKELPREQPLPPGPIGTERSQRTDRGPEPGPLRPAHRPGSQVEFGTTNKDSDLCLVVGDTLKGEKELVASATEAVPISRDWELLPSASTSAEPQPKSLGSGQCVPEPSPSGQRPYPEVFYGSPGPPNSQQVSGGAPIDSQLHPNSGGFRPGTPSLHQYRSQPLYLPPGPAPPSALLSGVALKGQFLDFSALQATELGKLPAGGVLYPPPSFLYSAAFCPSPLPDPPLLQVRQDLPSPSDFYSTPLQPGGQSGFLPSGAPAQQMLLPVVDSQLPVVNFGSLPPAPPPAPPPLSLLPVGPALQPPNLAVRPPPAPAARVLPSPARPFAPSLGRAELHPVELKPFQDYRKLSSNLGGPGSSRTPPSGRPFSGLNSRLKAPPSTYSGVFRTQRIDLYQQASPPDALRWMPKPWERAGPPSREGPPRRAEEPGSRGEKEPGLPPPR

Target ORF information:

RefSeq Version	XM_006524620.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524620.4

ORF Insert Sequence:

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGGCAAGTCC TTAGAGATCC AGAAACCCGC TGTTGCCCCT 
CGACATGGCC TGCAGAGTCT CGGAAAAGTT GCCATTGCCC GGCGCATGCC ACCCCCAGCC 
AACCTTCCAA GCCTGAAAGC CGAGAACAAA GGCAATGACC CCAATGTCTC ACTAGTGCCG 
AAAGACGGAA CAGGATGGGC AAGCAAACAG GAGCAGTCCG ACCCCAAGAG TTCCGATGCC 
TCAACCGCTC AGCCGCCGGA ATCGCAGCCA CTGCCGGCTT CACAGACGCC TGCCTCCAAC 
CAGCCGAAAC GACCCCCAAC AGCCCCCGAG AACACTCCTT CGGTTCCAAG CGGGGTAAAG 
TCCTGGGCAC AAGCCAGTGT CACCCATGGA GCCCATGGAG ATGGTGGAAG GGCATCAAAC 
CTACTGTCGC GATTCTCTCG AGAGGAATTT CCGACCCTGC AGGCGGCTGG AGACCAGGAC 
AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA CAGTCGTCTG GGCCCGGACC AAGCCTCCGC 
CCCCAAAATT CTACAACTTG GAGGGACGGA GGTGGGCGTG GCCCTGATGA CCTGGAGGGC 
CCGGACTCCA AACTTCATCA TGGCCATGAT CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA 
CCCCAGTTCC CTCCCTACCG CGGGATGATG CCGCCCTTTA TGTATCCCCC ATATCTCCCA 
TTCCCTCCGC CCTATGGACC CCAGGGGCCT TATAGATACC CGACTCCTGA TGGGCCCAGC 
CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC TCAGGGCCAC CAATGCGCCT TGTAGAGCCT 
GTGGGTCGGC CTTCTATTCT TAAAGAGGAT AATCTTAAAG AGTTTGACCA ACTGGACCAG 
GAAAATGATG ATGGTTGGGC AGGGGCCCAT GAAGAGGTTG ACTATACTGA AAAGCTCAAA 
TTCAGCGATG AGGAAGATGG ACGAGACTCC GACGAGGAGG GAGCTGAGGG CCACAAGGAT 
TCCCAGTCAG CTGCTGCTGA GGAACCTGAG ACAGATGGCA AAAAGGGCAC CTCCCCTGGC 
AGCGAATTGC CCCCTCCCAA GACAGCCTGG ACAGAGAATG CTCGGCCTTC AGAGACAGAG 
CCAGCTCCTC CTACCCCAAA GCCTCCCCCA CCTCCACCTC ACCGGGGGCC AGTCGGGAAC 
TGGGGGCCCC CTGGGGACTA CCCAGATCGA GGGGGTCCTC CCTGCAAACC CCCAGCACCT 
GAGGATGAGG ATGAGGCATG GCGGCAGCGT CGGAAGCAAT CTTCTTCAGA GATCTCCCTG 
GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA GAGGAAGAGC GACGCATGCA GGAGGAGCGC 
CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT CTTGATGAAA AGTTTGGGGC ACCTGACAAG 
CGGCTCAAGG CAGAGCCTGC AGCCCCACCT GTTACCCCTG CTGCCCCAGC TCTACCACCT 
GTAGTCCCCA AAGAAATTCC TGCAGCTCCA GCTCTGCCAC CAACACCTAC CCCAACACCA 
GAGAAAGAAC CTGAAGAGCC GGCACAAGCC CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG 
GTGGCTCCAG TTCCCACTCT GGTGAGTGGG GGTGGTTGCA CTGCCAACAG CAACAGCAGT 
GGCAGCTTCG AAGCCAGCCC AGTGGAACCC CAACTGCCTT CAAAAGAGGG TCCTGAACCA 
CCAGAAGAGG TTCCTCCACC TACCACACCC CCAGCCCCAA AGATGGAACC CAAAGGTGAT 
GGGGTTGGTT CTACTCGCCA GCCCCCCAGT CAGGGCTTGG GCTACCCCAA ATACCAGAAG 
TCATTGCCTC CTCGCTTCCA GCGGCAGCAA CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG 
CAACAGCAGT GGCAGCAGCA GCAACAAGGC ACTGCCCCTC CTGCCCCAGT GCCCCCATCA 
CCACCACAAC CTGTGACCCT GGGAGCTGTA CCTGCCCCAC AAGCACCGCC CCCACCTCCC 
AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG CCCCCACCAA TGCCTCCAAT GAACTTTGAC 
CCCCGCTGGA TGATGATTCC TCCTTACGTG GACCCTCGGC TTCTCCAGGG CCGGCCCCCT 
CTGGACTTCT ACCCTCCTGG TGTGCATCCC TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC 
AGTGGGGGCT CAAGCTCAGA GCCATTTGAA CGCCATGCAC CCCCTCTGTT GCGGGAAAGG 
GGCACCCCAC CAGTGGACCC CAAGTTGGCC TGGGTTGGAG ATGTCTTTAC CACCACACCC 
ACTGATCCTC GTCCTCTTAC CTCACCACTG CGCCAGGCAG CAGATGAGGA GGAGAAGAGT 
ATGAGGAGCG AGACTCCTCC GGTACCCCCG CCCCCACCTT ATCTGGCTAA TTATCCAGGT 
TTTCCAGAGA ATGGAACTCC TGGGCCCCCA ATCTCGAGAT TCCCTCTGGA GGAATCTGCT 
CCCCCGGGAC CCCGCCCACT ACCCTGGCCT CCAGGCAATG ATGAAGCTGC CAAGATGCAG 
GCTCCACCGC CCAAGAAGGA ACCCTCGAAG GAGGAACCGC CACAGCTGTC CGGGCCAGAA 
GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA GGCCCACCAC CACCACGGAG AGAGAATCGT 
ACTGAGACTC GCTGGGGCCC CCGACCAGGC AGTTGTCGCC GTGGAATCCC TCCAGAGGAG 
CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT ATAAAGAAGC CACCACCACC TGTGAAAGTA 
GAGGAGCTGC CTCCTAAGTC CCTAGAACAG GGGGATGAGA CCCCCAAGGT CCCAAAGCCA 
GATGCACTCA AGACAGCTAA GGGCAAGGTG GGCCCCAAAG AAACCCCACC AGGTGGAAAC 
CTGTCTCCTG CACCTAGGCT TCGGCGGGAC TATTCCTATG AAAGAGTAGG TCCTACATCT 
TGCCGTGGTC GGGGCAGAGG CGAGTATTTT GCCAGAGGAA GGGGTTTTCG GGGGACCTAT 
GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT GAATTCCGAA GTTACCGAGA GTTCCGAGGA 
GACGATGGGC GTGGGGGTGG TTCAGGCGGA ACAAACCATC CTTCTGCTCC CCGAGGCCGC 
ACTGCCAGTG AGACACGAAG TGAGGGTTCA GAATATGAAG AAATCCCCAA GAGACGCCGC 
CAGCGTGGCT CTGAAACAGG CAGTGAGACT CATGAGAGTG ACCTGGCCCC CTCTGACAAG 
GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT GGTCAGGTCC CTCTTGCCCC CCCACAACCA 
GGAGCTCCTC CATCACCAGC CCCAGCCCGA TTCTCCACTG CCCGAGGTGG TCGTGTCTTC 
ACTCCAAGAG GAGTACCATC ACGCCGTGGC CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC 
TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG GTTCCAAAGA AACCTCCAAC AGGTCCTTTG 
CCTCCAAGTA AAGAACCTTT GAAAGAGAAG CTGATCTCAG GGCCTCTGTC CCCCATGTCT 
CGAGCTGGCA ACATGGGTGT AGGCATGGAA GATGGGGAGC GACCCCGACG GAGGCGACAT 
GGCAGGGCTC AACAGCAGGA CAAACCACCT CGTTTCCGAA GGCTGAAACA AGAACGTGAA 
AACGCTGCCA GAGGAGCTGA TGGCAAGCCC CCTTCCCTAA CCCTTGCAGC CTCTACCCCT 
GGACCTGAGG AGACACTAAC AGCAGCCACA GTTCCCCCAC CCCCTCGCCG CACAGCTGCT 
AAGTCTCCTG ATCTGTCAAA CCAGAACTCA GACCAAGCCA ATGAGGAATG GGAGACTGCA 
TCAGAGAGCA GCGACTTTGC CAGTGAGCGC CGGGGTGACA AGGAGACTCC CCCAGCAGCA 
CTTATGACCT CTAAAGCTGT GGGGACTCCT GGGGCCAATG CAGGTGGGGC TGGACCAGGC 
ATTTCAGCCA TGTCTCGTGG AGACCTGAGC CAACGAGCCA AGGATTTGAG CAAGCGGAGC 
TTCTCAAGTC AGCGGCCAGG CATGGATCGT CAAAACCGGC GCCCAGGCAC TGGGGGCAAG 
ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT GGTGGTGCAG GCCCTGGGGG ACGGACCGGC 
CCAGGACGAG GGGATAAGAG GAGCTGGCCC TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG 
GAGCGACCTC CGGGACTTCC TCTGCCTCCG CCACCCCCCA GCAGTTCTGC TGTCTTCCGC 
CTGGACCAAG TTATCCATAG CAACCCTGCT GGAATCCAAC AGGCTCTGGC CCAACTTAGC 
AGCCGCCAAG GAAATGTAAC TGCACCAGGG GGGCACCCAA GGCCTAAGCC TGGGCCTCCC 
CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC CCAACCCGTT ATGACCCCCC AAGGGCCAGC 
AGCGCTATCA GTTCTGACCC CCACTTCGAG GAGCCAGGGC CGATGGTGAG GGGGGTGGGC 
GGGACTCCCC GGGACTCTGC CGGGGTTAAT CCCTTCCCCC CGAAACGGCG GGAGCGGCCT 
CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA ACTGTGCCAG CTTCTCATAG CTCTGGATTC 
TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT CAGGAAGAAT CCAGAGATTC AGGCACAGAG 
GCCCTAACTC CCCACATCTG GAATCGTTTA CATACTGCCA CAAGCCGAAA GAGTTACCAG 
CCTGGCTCCA TCGAGCCCTG GATGGAGCCC CTGAGTCCCT TTGAGGATGT GGCAGGCACA 
GAGATGAGTC AGTCTGACAG TGGAGTGGAC CTGAGTGGAG ATTCTCAGGT GTCATCAGGT 
CCCTGCAGTC AGCGAAGTTC CCCTGATGGA GGACTTAAGG GGTCAGCAGA GGGACCCCCC 
AAGCGGCCTG GAGGACCCTC ACCCCTGAAT GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT 
GAACCCTCTG AACCTCCTAG GAGACGGCCA CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG 
CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT CCCATAGGCA CAGAACGATC ACAGCGCACA 
GATCGAGGCC CAGAGCCAGG CCCTCTCCGA CCAGCCCACC GACCGGGCTC TCAAGTTGAG 
TTTGGCACTA CCAACAAGGA CTCAGACTTG TGCCTAGTGG TCGGAGACAC TTTAAAAGGA 
GAAAAGGAGT TGGTGGCATC AGCCACAGAG GCCGTTCCCA TATCCAGAGA CTGGGAGCTG 
CTCCCTAGTG CTTCTACTTC AGCTGAGCCA CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT 
GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT CCGTACCCTG AGGTCTTCTA TGGCAGCCCT 
GGACCTCCTA ATTCCCAGCA GGTCTCTGGG GGTGCACCCA TCGACTCTCA GTTACATCCC 
AACAGTGGAG GCTTCCGTCC TGGGACACCC TCATTGCACC AGTACAGGTC ACAGCCTCTG 
TATCTGCCCC CAGGTCCAGC CCCTCCCTCG GCACTGCTTT CTGGGGTAGC TCTCAAGGGC 
CAGTTTCTGG ATTTCTCAGC ACTGCAAGCC ACAGAGCTGG GGAAGTTGCC TGCTGGGGGA 
GTTCTCTACC CTCCGCCATC CTTCCTCTAC TCTGCAGCTT TCTGCCCCAG CCCTTTGCCT 
GACCCACCCT TGCTTCAGGT ACGCCAGGAT CTGCCATCCC CTTCAGATTT TTACTCTACT 
CCTCTGCAGC CTGGTGGCCA GAGTGGCTTC CTTCCTTCGG GAGCCCCTGC TCAGCAGATG 
CTTCTACCTG TGGTAGACTC ACAGCTGCCA GTGGTGAACT TTGGCTCCCT GCCACCAGCG 
CCACCCCCTG CTCCACCCCC CCTTTCTCTG TTACCTGTGG GTCCTGCTCT CCAGCCCCCC 
AACCTAGCTG TGCGGCCCCC ACCTGCTCCT GCTGCTCGGG TCTTACCTTC ACCCGCTAGA 
CCCTTCGCCC CTAGCTTGGG GCGAGCAGAG CTGCACCCAG TAGAACTAAA GCCTTTCCAG 
GATTACCGAA AGCTGAGCAG CAACCTTGGG GGTCCTGGGT CGTCACGGAC TCCCCCATCT 
GGAAGGCCCT TCTCTGGTCT CAATTCCCGT CTCAAGGCCC CACCTTCCAC CTACAGTGGA 
GTATTCCGAA CTCAGCGCAT TGACCTCTAC CAGCAGGCTT CCCCACCAGA TGCCCTGCGC 
TGGATGCCGA AGCCTTGGGA ACGGGCAGGG CCACCTTCTC GAGAAGGGCC TCCCCGAAGA 
GCAGAGGAGC CTGGGTCCCG GGGGGAGAAG GAGCCTGGAT TGCCCCCACC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prrc2a ( XM_030249899.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105759.1 Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X2, mRNA.

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CloneID	OMu15766
Clone ID Related Accession (Same CDS sequence)	NM_001199044.1 , XM_030249899.1
Accession Version	XM_030249899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein PRRC2A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGGCAAGTCC TTAGAGATCC AGAAACCCGC TGTTGCCCCT 
CGACATGGCC TGCAGAGTCT CGGAAAAGTT GCCATTGCCC GGCGCATGCC ACCCCCAGCC 
AACCTTCCAA GCCTGAAAGC CGAGAACAAA GGCAATGACC CCAATGTCTC ACTAGTGCCG 
AAAGACGGAA CAGGATGGGC AAGCAAACAG GAGCAGTCCG ACCCCAAGAG TTCCGATGCC 
TCAACCGCTC AGCCGCCGGA ATCGCAGCCA CTGCCGGCTT CACAGACGCC TGCCTCCAAC 
CAGCCGAAAC GACCCCCAAC AGCCCCCGAG AACACTCCTT CGGTTCCAAG CGGGGTAAAG 
TCCTGGGCAC AAGCCAGTGT CACCCATGGA GCCCATGGAG ATGGTGGAAG GGCATCAAAC 
CTACTGTCGC GATTCTCTCG AGAGGAATTT CCGACCCTGC AGGCGGCTGG AGACCAGGAC 
AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA CAGTCGTCTG GGCCCGGACC AAGCCTCCGC 
CCCCAAAATT CTACAACTTG GAGGGACGGA GGTGGGCGTG GCCCTGATGA CCTGGAGGGC 
CCGGACTCCA AACTTCATCA TGGCCATGAT CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA 
CCCCAGTTCC CTCCCTACCG CGGGATGATG CCGCCCTTTA TGTATCCCCC ATATCTCCCA 
TTCCCTCCGC CCTATGGACC CCAGGGGCCT TATAGATACC CGACTCCTGA TGGGCCCAGC 
CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC TCAGGGCCAC CAATGCGCCT TGTAGAGCCT 
GTGGGTCGGC CTTCTATTCT TAAAGAGGAT AATCTTAAAG AGTTTGACCA ACTGGACCAG 
GAAAATGATG ATGGTTGGGC AGGGGCCCAT GAAGAGGTTG ACTATACTGA AAAGCTCAAA 
TTCAGCGATG AGGAAGATGG ACGAGACTCC GACGAGGAGG GAGCTGAGGG CCACAAGGAT 
TCCCAGTCAG CTGCTGCTGA GGAACCTGAG ACAGATGGCA AAAAGGGCAC CTCCCCTGGC 
AGCGAATTGC CCCCTCCCAA GACAGCCTGG ACAGAGAATG CTCGGCCTTC AGAGACAGAG 
CCAGCTCCTC CTACCCCAAA GCCTCCCCCA CCTCCACCTC ACCGGGGGCC AGTCGGGAAC 
TGGGGGCCCC CTGGGGACTA CCCAGATCGA GGGGGTCCTC CCTGCAAACC CCCAGCACCT 
GAGGATGAGG ATGAGGCATG GCGGCAGCGT CGGAAGCAAT CTTCTTCAGA GATCTCCCTG 
GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA GAGGAAGAGC GACGCATGCA GGAGGAGCGC 
CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT CTTGATGAAA AGTTTGGGGC ACCTGACAAG 
CGGCTCAAGG CAGAGCCTGC AGCCCCACCT GTTACCCCTG CTGCCCCAGC TCTACCACCT 
GTAGTCCCCA AAGAAATTCC TGCAGCTCCA GCTCTGCCAC CAACACCTAC CCCAACACCA 
GAGAAAGAAC CTGAAGAGCC GGCACAAGCC CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG 
GTGGCTCCAG TTCCCACTCT GGTGAGTGGG GGTGGTTGCA CTGCCAACAG CAACAGCAGT 
GGCAGCTTCG AAGCCAGCCC AGTGGAACCC CAACTGCCTT CAAAAGAGGG TCCTGAACCA 
CCAGAAGAGG TTCCTCCACC TACCACACCC CCAGCCCCAA AGATGGAACC CAAAGGTGAT 
GGGGTTGGTT CTACTCGCCA GCCCCCCAGT CAGGGCTTGG GCTACCCCAA ATACCAGAAG 
TCATTGCCTC CTCGCTTCCA GCGGCAGCAA CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG 
CAACAGCAGT GGCAGCAGCA GCAACAAGGC ACTGCCCCTC CTGCCCCAGT GCCCCCATCA 
CCACCACAAC CTGTGACCCT GGGAGCTGTA CCTGCCCCAC AAGCACCGCC CCCACCTCCC 
AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG CCCCCACCAA TGCCTCCAAT GAACTTTGAC 
CCCCGCTGGA TGATGATTCC TCCTTACGTG GACCCTCGGC TTCTCCAGGG CCGGCCCCCT 
CTGGACTTCT ACCCTCCTGG TGTGCATCCC TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC 
AGTGGGGGCT CAAGCTCAGA GCCATTTGAA CGCCATGCAC CCCCTCTGTT GCGGGAAAGG 
GGCACCCCAC CAGTGGACCC CAAGTTGGCC TGGGTTGGAG ATGTCTTTAC CACCACACCC 
ACTGATCCTC GTCCTCTTAC CTCACCACTG CGCCAGGCAG CAGATGAGGA GGAGAAGAGT 
ATGAGGAGCG AGACTCCTCC GGTACCCCCG CCCCCACCTT ATCTGGCTAA TTATCCAGGT 
TTTCCAGAGA ATGGAACTCC TGGGCCCCCA ATCTCGAGAT TCCCTCTGGA GGAATCTGCT 
CCCCCGGGAC CCCGCCCACT ACCCTGGCCT CCAGGCAATG ATGAAGCTGC CAAGATGCAG 
GCTCCACCGC CCAAGAAGGA ACCCTCGAAG GAGGAACCGC CACAGCTGTC CGGGCCAGAA 
GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA GGCCCACCAC CACCACGGAG AGAGAATCGT 
ACTGAGACTC GCTGGGGCCC CCGACCAGGC AGTTGTCGCC GTGGAATCCC TCCAGAGGAG 
CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT ATAAAGAAGC CACCACCACC TGTGAAAGTA 
GAGGAGCTGC CTCCTAAGTC CCTAGAACAG GGGGATGAGA CCCCCAAGGT CCCAAAGCCA 
GATGCACTCA AGACAGCTAA GGGCAAGGTG GGCCCCAAAG AAACCCCACC AGGTGGAAAC 
CTGTCTCCTG CACCTAGGCT TCGGCGGGAC TATTCCTATG AAAGAGTAGG TCCTACATCT 
TGCCGTGGTC GGGGCAGAGG CGAGTATTTT GCCAGAGGAA GGGGTTTTCG GGGGACCTAT 
GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT GAATTCCGAA GTTACCGAGA GTTCCGAGGA 
GACGATGGGC GTGGGGGTGG TTCAGGCGGA ACAAACCATC CTTCTGCTCC CCGAGGCCGC 
ACTGCCAGTG AGACACGAAG TGAGGGTTCA GAATATGAAG AAATCCCCAA GAGACGCCGC 
CAGCGTGGCT CTGAAACAGG CAGTGAGACT CATGAGAGTG ACCTGGCCCC CTCTGACAAG 
GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT GGTCAGGTCC CTCTTGCCCC CCCACAACCA 
GGAGCTCCTC CATCACCAGC CCCAGCCCGA TTCTCCACTG CCCGAGGTGG TCGTGTCTTC 
ACTCCAAGAG GAGTACCATC ACGCCGTGGC CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC 
TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG GTTCCAAAGA AACCTCCAAC AGGTCCTTTG 
CCTCCAAGTA AAGAACCTTT GAAAGAGAAG CTGATCTCAG GGCCTCTGTC CCCCATGTCT 
CGAGCTGGCA ACATGGGTGT AGGCATGGAA GATGGGGAGC GACCCCGACG GAGGCGACAT 
GGCAGGGCTC AACAGCAGGA CAAACCACCT CGTTTCCGAA GGCTGAAACA AGAACGTGAA 
AACGCTGCCA GAGGAGCTGA TGGCAAGCCC CCTTCCCTAA CCCTTGCAGC CTCTACCCCT 
GGACCTGAGG AGACACTAAC AGCAGCCACA GTTCCCCCAC CCCCTCGCCG CACAGCTGCT 
AAGTCTCCTG ATCTGTCAAA CCAGAACTCA GACCAAGCCA ATGAGGAATG GGAGACTGCA 
TCAGAGAGCA GCGACTTTGC CAGTGAGCGC CGGGGTGACA AGGAGACTCC CCCAGCAGCA 
CTTATGACCT CTAAAGCTGT GGGGACTCCT GGGGCCAATG CAGGTGGGGC TGGACCAGGC 
ATTTCAGCCA TGTCTCGTGG AGACCTGAGC CAACGAGCCA AGGATTTGAG CAAGCGGAGC 
TTCTCAAGTC AGCGGCCAGG CATGGATCGT CAAAACCGGC GCCCAGGCAC TGGGGGCAAG 
ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT GGTGGTGCAG GCCCTGGGGG ACGGACCGGC 
CCAGGACGAG GGGATAAGAG GAGCTGGCCC TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG 
GAGCGACCTC CGGGACTTCC TCTGCCTCCG CCACCCCCCA GCAGTTCTGC TGTCTTCCGC 
CTGGACCAAG TTATCCATAG CAACCCTGCT GGAATCCAAC AGGCTCTGGC CCAACTTAGC 
AGCCGCCAAG GAAATGTAAC TGCACCAGGG GGGCACCCAA GGCCTAAGCC TGGGCCTCCC 
CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC CCAACCCGTT ATGACCCCCC AAGGGCCAGC 
AGCGCTATCA GTTCTGACCC CCACTTCGAG GAGCCAGGGC CGATGGTGAG GGGGGTGGGC 
GGGACTCCCC GGGACTCTGC CGGGGTTAAT CCCTTCCCCC CGAAACGGCG GGAGCGGCCT 
CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA ACTGTGCCAG CTTCTCATAG CTCTGGATTC 
TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT CAGGAAGAAT CCAGAGATTC AGGCACAGAG 
GCCCTAACTC CCCACATCTG GAATCGTTTA CATACTGCCA CAAGCCGAAA GAGTTACCAG 
CCTGGCTCCA TCGAGCCCTG GATGGAGCCC CTGAGTCCCT TTGAGGATGT GGCAGGCACA 
GAGATGAGTC AGTCTGACAG TGGAGTGGAC CTGAGTGGAG ATTCTCAGGT GTCATCAGGT 
CCCTGCAGTC AGCGAAGTTC CCCTGATGGA GGACTTAAGG GGTCAGCAGA GGGACCCCCC 
AAGCGGCCTG GAGGACCCTC ACCCCTGAAT GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT 
GAACCCTCTG AACCTCCTAG GAGACGGCCA CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG 
CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT CCCATAGGCA CAGAACGATC ACAGCGCACA 
GATCGAGGCC CAGAGCCAGG CCCTCTCCGA CCAGCCCACC GACCGGGCTC TCAAGTTGAG 
TTTGGCACTA CCAACAAGGA CTCAGACTTG TGCCTAGTGG TCGGAGACAC TTTAAAAGGA 
GAAAAGGAGT TGGTGGCATC AGCCACAGAG GCCGTTCCCA TATCCAGAGA CTGGGAGCTG 
CTCCCTAGTG CTTCTACTTC AGCTGAGCCA CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT 
GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT CCGTACCCTG AGGTCTTCTA TGGCAGCCCT 
GGACCTCCTA ATTCCCAGGT CTCTGGGGGT GCACCCATCG ACTCTCAGTT ACATCCCAAC 
AGTGGAGGCT TCCGTCCTGG GACACCCTCA TTGCACCAGT ACAGGTCACA GCCTCTGTAT 
CTGCCCCCAG GTCCAGCCCC TCCCTCGGCA CTGCTTTCTG GGGTAGCTCT CAAGGGCCAG 
TTTCTGGATT TCTCAGCACT GCAAGCCACA GAGCTGGGGA AGTTGCCTGC TGGGGGAGTT 
CTCTACCCTC CGCCATCCTT CCTCTACTCT GCAGCTTTCT GCCCCAGCCC TTTGCCTGAC 
CCACCCTTGC TTCAGGTACG CCAGGATCTG CCATCCCCTT CAGATTTTTA CTCTACTCCT 
CTGCAGCCTG GTGGCCAGAG TGGCTTCCTT CCTTCGGGAG CCCCTGCTCA GCAGATGCTT 
CTACCTGTGG TAGACTCACA GCTGCCAGTG GTGAACTTTG GCTCCCTGCC ACCAGCGCCA 
CCCCCTGCTC CACCCCCCCT TTCTCTGTTA CCTGTGGGTC CTGCTCTCCA GCCCCCCAAC 
CTAGCTGTGC GGCCCCCACC TGCTCCTGCT GCTCGGGTCT TACCTTCACC CGCTAGACCC 
TTCGCCCCTA GCTTGGGGCG AGCAGAGCTG CACCCAGTAG AACTAAAGCC TTTCCAGGAT 
TACCGAAAGC TGAGCAGCAA CCTTGGGGGT CCTGGGTCGT CACGGACTCC CCCATCTGGA 
AGGCCCTTCT CTGGTCTCAA TTCCCGTCTC AAGGCCCCAC CTTCCACCTA CAGTGGAGTA 
TTCCGAACTC AGCGCATTGA CCTCTACCAG CAGGCTTCCC CACCAGATGC CCTGCGCTGG 
ATGCCGAAGC CTTGGGAACG GGCAGGGCCA CCTTCTCGAG AAGGGCCTCC CCGAAGAGCA 
GAGGAGCCTG GGTCCCGGGG GGAGAAGGAG CCTGGATTGC CCCCACCCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105759.1
CDS	312..6785
Translation	MSDRSGPTAKGKDGKKYSSLNLFDTYKGKSLEIQKPAVAPRHGLQSLGKVAIARRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQSDPKSSDASTAQPPESQPLPASQTPASNQPKRPPTAPENTPSVPSGVKSWAQASVTHGAHGDGGRASNLLSRFSREEFPTLQAAGDQDKAAKERESAEQSSGPGPSLRPQNSTTWRDGGGRGPDDLEGPDSKLHHGHDPRGGLQPSGPPQFPPYRGMMPPFMYPPYLPFPPPYGPQGPYRYPTPDGPSRFPRVAGPRGSGPPMRLVEPVGRPSILKEDNLKEFDQLDQENDDGWAGAHEEVDYTEKLKFSDEEDGRDSDEEGAEGHKDSQSAAAEEPETDGKKGTSPGSELPPPKTAWTENARPSETEPAPPTPKPPPPPPHRGPVGNWGPPGDYPDRGGPPCKPPAPEDEDEAWRQRRKQSSSEISLAVERARRRREEEERRMQEERRAACAEKLKRLDEKFGAPDKRLKAEPAAPPVTPAAPALPPVVPKEIPAAPALPPTPTPTPEKEPEEPAQAPPVQAAPSPGVAPVPTLVSGGGCTANSNSSGSFEASPVEPQLPSKEGPEPPEEVPPPTTPPAPKMEPKGDGVGSTRQPPSQGLGYPKYQKSLPPRFQRQQQEQLLKQQQQQQQWQQQQQGTAPPAPVPPSPPQPVTLGAVPAPQAPPPPPKALYPGALGRPPPMPPMNFDPRWMMIPPYVDPRLLQGRPPLDFYPPGVHPSGLVPRERSDSGGSSSEPFERHAPPLLRERGTPPVDPKLAWVGDVFTTTPTDPRPLTSPLRQAADEEEKSMRSETPPVPPPPPYLANYPGFPENGTPGPPISRFPLEESAPPGPRPLPWPPGNDEAAKMQAPPPKKEPSKEEPPQLSGPEAGRKPARGGQGPPPPRRENRTETRWGPRPGSCRRGIPPEEPGVPPRRAGPIKKPPPPVKVEELPPKSLEQGDETPKVPKPDALKTAKGKVGPKETPPGGNLSPAPRLRRDYSYERVGPTSCRGRGRGEYFARGRGFRGTYGGRGRGARSREFRSYREFRGDDGRGGGSGGTNHPSAPRGRTASETRSEGSEYEEIPKRRRQRGSETGSETHESDLAPSDKEAPPPKEGVLGQVPLAPPQPGAPPSPAPARFSTARGGRVFTPRGVPSRRGRGGGRPPPVCSGWSPPAKSLVPKKPPTGPLPPSKEPLKEKLISGPLSPMSRAGNMGVGMEDGERPRRRRHGRAQQQDKPPRFRRLKQERENAARGADGKPPSLTLAASTPGPEETLTAATVPPPPRRTAAKSPDLSNQNSDQANEEWETASESSDFASERRGDKETPPAALMTSKAVGTPGANAGGAGPGISAMSRGDLSQRAKDLSKRSFSSQRPGMDRQNRRPGTGGKTGSGGGSSGGGGAGPGGRTGPGRGDKRSWPSPKNRSRPPEERPPGLPLPPPPPSSSAVFRLDQVIHSNPAGIQQALAQLSSRQGNVTAPGGHPRPKPGPPQAPQGSSPRPPTRYDPPRASSAISSDPHFEEPGPMVRGVGGTPRDSAGVNPFPPKRRERPPRKPELLQEETVPASHSSGFLGSKPEVPGPQEESRDSGTEALTPHIWNRLHTATSRKSYQPGSIEPWMEPLSPFEDVAGTEMSQSDSGVDLSGDSQVSSGPCSQRSSPDGGLKGSAEGPPKRPGGPSPLNAVPGESASGSEPSEPPRRRPPASHEGERKELPREQPLPPGPIGTERSQRTDRGPEPGPLRPAHRPGSQVEFGTTNKDSDLCLVVGDTLKGEKELVASATEAVPISRDWELLPSASTSAEPQPKSLGSGQCVPEPSPSGQRPYPEVFYGSPGPPNSQVSGGAPIDSQLHPNSGGFRPGTPSLHQYRSQPLYLPPGPAPPSALLSGVALKGQFLDFSALQATELGKLPAGGVLYPPPSFLYSAAFCPSPLPDPPLLQVRQDLPSPSDFYSTPLQPGGQSGFLPSGAPAQQMLLPVVDSQLPVVNFGSLPPAPPPAPPPLSLLPVGPALQPPNLAVRPPPAPAARVLPSPARPFAPSLGRAELHPVELKPFQDYRKLSSNLGGPGSSRTPPSGRPFSGLNSRLKAPPSTYSGVFRTQRIDLYQQASPPDALRWMPKPWERAGPPSREGPPRRAEEPGSRGEKEPGLPPPR

Target ORF information:

RefSeq Version	XM_030249899.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249899.1

ORF Insert Sequence:

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGGCAAGTCC TTAGAGATCC AGAAACCCGC TGTTGCCCCT 
CGACATGGCC TGCAGAGTCT CGGAAAAGTT GCCATTGCCC GGCGCATGCC ACCCCCAGCC 
AACCTTCCAA GCCTGAAAGC CGAGAACAAA GGCAATGACC CCAATGTCTC ACTAGTGCCG 
AAAGACGGAA CAGGATGGGC AAGCAAACAG GAGCAGTCCG ACCCCAAGAG TTCCGATGCC 
TCAACCGCTC AGCCGCCGGA ATCGCAGCCA CTGCCGGCTT CACAGACGCC TGCCTCCAAC 
CAGCCGAAAC GACCCCCAAC AGCCCCCGAG AACACTCCTT CGGTTCCAAG CGGGGTAAAG 
TCCTGGGCAC AAGCCAGTGT CACCCATGGA GCCCATGGAG ATGGTGGAAG GGCATCAAAC 
CTACTGTCGC GATTCTCTCG AGAGGAATTT CCGACCCTGC AGGCGGCTGG AGACCAGGAC 
AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA CAGTCGTCTG GGCCCGGACC AAGCCTCCGC 
CCCCAAAATT CTACAACTTG GAGGGACGGA GGTGGGCGTG GCCCTGATGA CCTGGAGGGC 
CCGGACTCCA AACTTCATCA TGGCCATGAT CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA 
CCCCAGTTCC CTCCCTACCG CGGGATGATG CCGCCCTTTA TGTATCCCCC ATATCTCCCA 
TTCCCTCCGC CCTATGGACC CCAGGGGCCT TATAGATACC CGACTCCTGA TGGGCCCAGC 
CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC TCAGGGCCAC CAATGCGCCT TGTAGAGCCT 
GTGGGTCGGC CTTCTATTCT TAAAGAGGAT AATCTTAAAG AGTTTGACCA ACTGGACCAG 
GAAAATGATG ATGGTTGGGC AGGGGCCCAT GAAGAGGTTG ACTATACTGA AAAGCTCAAA 
TTCAGCGATG AGGAAGATGG ACGAGACTCC GACGAGGAGG GAGCTGAGGG CCACAAGGAT 
TCCCAGTCAG CTGCTGCTGA GGAACCTGAG ACAGATGGCA AAAAGGGCAC CTCCCCTGGC 
AGCGAATTGC CCCCTCCCAA GACAGCCTGG ACAGAGAATG CTCGGCCTTC AGAGACAGAG 
CCAGCTCCTC CTACCCCAAA GCCTCCCCCA CCTCCACCTC ACCGGGGGCC AGTCGGGAAC 
TGGGGGCCCC CTGGGGACTA CCCAGATCGA GGGGGTCCTC CCTGCAAACC CCCAGCACCT 
GAGGATGAGG ATGAGGCATG GCGGCAGCGT CGGAAGCAAT CTTCTTCAGA GATCTCCCTG 
GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA GAGGAAGAGC GACGCATGCA GGAGGAGCGC 
CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT CTTGATGAAA AGTTTGGGGC ACCTGACAAG 
CGGCTCAAGG CAGAGCCTGC AGCCCCACCT GTTACCCCTG CTGCCCCAGC TCTACCACCT 
GTAGTCCCCA AAGAAATTCC TGCAGCTCCA GCTCTGCCAC CAACACCTAC CCCAACACCA 
GAGAAAGAAC CTGAAGAGCC GGCACAAGCC CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG 
GTGGCTCCAG TTCCCACTCT GGTGAGTGGG GGTGGTTGCA CTGCCAACAG CAACAGCAGT 
GGCAGCTTCG AAGCCAGCCC AGTGGAACCC CAACTGCCTT CAAAAGAGGG TCCTGAACCA 
CCAGAAGAGG TTCCTCCACC TACCACACCC CCAGCCCCAA AGATGGAACC CAAAGGTGAT 
GGGGTTGGTT CTACTCGCCA GCCCCCCAGT CAGGGCTTGG GCTACCCCAA ATACCAGAAG 
TCATTGCCTC CTCGCTTCCA GCGGCAGCAA CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG 
CAACAGCAGT GGCAGCAGCA GCAACAAGGC ACTGCCCCTC CTGCCCCAGT GCCCCCATCA 
CCACCACAAC CTGTGACCCT GGGAGCTGTA CCTGCCCCAC AAGCACCGCC CCCACCTCCC 
AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG CCCCCACCAA TGCCTCCAAT GAACTTTGAC 
CCCCGCTGGA TGATGATTCC TCCTTACGTG GACCCTCGGC TTCTCCAGGG CCGGCCCCCT 
CTGGACTTCT ACCCTCCTGG TGTGCATCCC TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC 
AGTGGGGGCT CAAGCTCAGA GCCATTTGAA CGCCATGCAC CCCCTCTGTT GCGGGAAAGG 
GGCACCCCAC CAGTGGACCC CAAGTTGGCC TGGGTTGGAG ATGTCTTTAC CACCACACCC 
ACTGATCCTC GTCCTCTTAC CTCACCACTG CGCCAGGCAG CAGATGAGGA GGAGAAGAGT 
ATGAGGAGCG AGACTCCTCC GGTACCCCCG CCCCCACCTT ATCTGGCTAA TTATCCAGGT 
TTTCCAGAGA ATGGAACTCC TGGGCCCCCA ATCTCGAGAT TCCCTCTGGA GGAATCTGCT 
CCCCCGGGAC CCCGCCCACT ACCCTGGCCT CCAGGCAATG ATGAAGCTGC CAAGATGCAG 
GCTCCACCGC CCAAGAAGGA ACCCTCGAAG GAGGAACCGC CACAGCTGTC CGGGCCAGAA 
GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA GGCCCACCAC CACCACGGAG AGAGAATCGT 
ACTGAGACTC GCTGGGGCCC CCGACCAGGC AGTTGTCGCC GTGGAATCCC TCCAGAGGAG 
CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT ATAAAGAAGC CACCACCACC TGTGAAAGTA 
GAGGAGCTGC CTCCTAAGTC CCTAGAACAG GGGGATGAGA CCCCCAAGGT CCCAAAGCCA 
GATGCACTCA AGACAGCTAA GGGCAAGGTG GGCCCCAAAG AAACCCCACC AGGTGGAAAC 
CTGTCTCCTG CACCTAGGCT TCGGCGGGAC TATTCCTATG AAAGAGTAGG TCCTACATCT 
TGCCGTGGTC GGGGCAGAGG CGAGTATTTT GCCAGAGGAA GGGGTTTTCG GGGGACCTAT 
GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT GAATTCCGAA GTTACCGAGA GTTCCGAGGA 
GACGATGGGC GTGGGGGTGG TTCAGGCGGA ACAAACCATC CTTCTGCTCC CCGAGGCCGC 
ACTGCCAGTG AGACACGAAG TGAGGGTTCA GAATATGAAG AAATCCCCAA GAGACGCCGC 
CAGCGTGGCT CTGAAACAGG CAGTGAGACT CATGAGAGTG ACCTGGCCCC CTCTGACAAG 
GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT GGTCAGGTCC CTCTTGCCCC CCCACAACCA 
GGAGCTCCTC CATCACCAGC CCCAGCCCGA TTCTCCACTG CCCGAGGTGG TCGTGTCTTC 
ACTCCAAGAG GAGTACCATC ACGCCGTGGC CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC 
TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG GTTCCAAAGA AACCTCCAAC AGGTCCTTTG 
CCTCCAAGTA AAGAACCTTT GAAAGAGAAG CTGATCTCAG GGCCTCTGTC CCCCATGTCT 
CGAGCTGGCA ACATGGGTGT AGGCATGGAA GATGGGGAGC GACCCCGACG GAGGCGACAT 
GGCAGGGCTC AACAGCAGGA CAAACCACCT CGTTTCCGAA GGCTGAAACA AGAACGTGAA 
AACGCTGCCA GAGGAGCTGA TGGCAAGCCC CCTTCCCTAA CCCTTGCAGC CTCTACCCCT 
GGACCTGAGG AGACACTAAC AGCAGCCACA GTTCCCCCAC CCCCTCGCCG CACAGCTGCT 
AAGTCTCCTG ATCTGTCAAA CCAGAACTCA GACCAAGCCA ATGAGGAATG GGAGACTGCA 
TCAGAGAGCA GCGACTTTGC CAGTGAGCGC CGGGGTGACA AGGAGACTCC CCCAGCAGCA 
CTTATGACCT CTAAAGCTGT GGGGACTCCT GGGGCCAATG CAGGTGGGGC TGGACCAGGC 
ATTTCAGCCA TGTCTCGTGG AGACCTGAGC CAACGAGCCA AGGATTTGAG CAAGCGGAGC 
TTCTCAAGTC AGCGGCCAGG CATGGATCGT CAAAACCGGC GCCCAGGCAC TGGGGGCAAG 
ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT GGTGGTGCAG GCCCTGGGGG ACGGACCGGC 
CCAGGACGAG GGGATAAGAG GAGCTGGCCC TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG 
GAGCGACCTC CGGGACTTCC TCTGCCTCCG CCACCCCCCA GCAGTTCTGC TGTCTTCCGC 
CTGGACCAAG TTATCCATAG CAACCCTGCT GGAATCCAAC AGGCTCTGGC CCAACTTAGC 
AGCCGCCAAG GAAATGTAAC TGCACCAGGG GGGCACCCAA GGCCTAAGCC TGGGCCTCCC 
CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC CCAACCCGTT ATGACCCCCC AAGGGCCAGC 
AGCGCTATCA GTTCTGACCC CCACTTCGAG GAGCCAGGGC CGATGGTGAG GGGGGTGGGC 
GGGACTCCCC GGGACTCTGC CGGGGTTAAT CCCTTCCCCC CGAAACGGCG GGAGCGGCCT 
CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA ACTGTGCCAG CTTCTCATAG CTCTGGATTC 
TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT CAGGAAGAAT CCAGAGATTC AGGCACAGAG 
GCCCTAACTC CCCACATCTG GAATCGTTTA CATACTGCCA CAAGCCGAAA GAGTTACCAG 
CCTGGCTCCA TCGAGCCCTG GATGGAGCCC CTGAGTCCCT TTGAGGATGT GGCAGGCACA 
GAGATGAGTC AGTCTGACAG TGGAGTGGAC CTGAGTGGAG ATTCTCAGGT GTCATCAGGT 
CCCTGCAGTC AGCGAAGTTC CCCTGATGGA GGACTTAAGG GGTCAGCAGA GGGACCCCCC 
AAGCGGCCTG GAGGACCCTC ACCCCTGAAT GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT 
GAACCCTCTG AACCTCCTAG GAGACGGCCA CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG 
CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT CCCATAGGCA CAGAACGATC ACAGCGCACA 
GATCGAGGCC CAGAGCCAGG CCCTCTCCGA CCAGCCCACC GACCGGGCTC TCAAGTTGAG 
TTTGGCACTA CCAACAAGGA CTCAGACTTG TGCCTAGTGG TCGGAGACAC TTTAAAAGGA 
GAAAAGGAGT TGGTGGCATC AGCCACAGAG GCCGTTCCCA TATCCAGAGA CTGGGAGCTG 
CTCCCTAGTG CTTCTACTTC AGCTGAGCCA CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT 
GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT CCGTACCCTG AGGTCTTCTA TGGCAGCCCT 
GGACCTCCTA ATTCCCAGGT CTCTGGGGGT GCACCCATCG ACTCTCAGTT ACATCCCAAC 
AGTGGAGGCT TCCGTCCTGG GACACCCTCA TTGCACCAGT ACAGGTCACA GCCTCTGTAT 
CTGCCCCCAG GTCCAGCCCC TCCCTCGGCA CTGCTTTCTG GGGTAGCTCT CAAGGGCCAG 
TTTCTGGATT TCTCAGCACT GCAAGCCACA GAGCTGGGGA AGTTGCCTGC TGGGGGAGTT 
CTCTACCCTC CGCCATCCTT CCTCTACTCT GCAGCTTTCT GCCCCAGCCC TTTGCCTGAC 
CCACCCTTGC TTCAGGTACG CCAGGATCTG CCATCCCCTT CAGATTTTTA CTCTACTCCT 
CTGCAGCCTG GTGGCCAGAG TGGCTTCCTT CCTTCGGGAG CCCCTGCTCA GCAGATGCTT 
CTACCTGTGG TAGACTCACA GCTGCCAGTG GTGAACTTTG GCTCCCTGCC ACCAGCGCCA 
CCCCCTGCTC CACCCCCCCT TTCTCTGTTA CCTGTGGGTC CTGCTCTCCA GCCCCCCAAC 
CTAGCTGTGC GGCCCCCACC TGCTCCTGCT GCTCGGGTCT TACCTTCACC CGCTAGACCC 
TTCGCCCCTA GCTTGGGGCG AGCAGAGCTG CACCCAGTAG AACTAAAGCC TTTCCAGGAT 
TACCGAAAGC TGAGCAGCAA CCTTGGGGGT CCTGGGTCGT CACGGACTCC CCCATCTGGA 
AGGCCCTTCT CTGGTCTCAA TTCCCGTCTC AAGGCCCCAC CTTCCACCTA CAGTGGAGTA 
TTCCGAACTC AGCGCATTGA CCTCTACCAG CAGGCTTCCC CACCAGATGC CCTGCGCTGG 
ATGCCGAAGC CTTGGGAACG GGCAGGGCCA CCTTCTCGAG AAGGGCCTCC CCGAAGAGCA 
GAGGAGCCTG GGTCCCGGGG GGAGAAGGAG CCTGGATTGC CCCCACCCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prrc2a ( XM_030249900.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105760.1 Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X3, mRNA.

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CloneID	OMu53832
Clone ID Related Accession (Same CDS sequence)	XM_006524621.3 , XM_030249900.1 , XM_006524621.4
Accession Version	XM_030249900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein PRRC2A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGTTGCCCCT CGACATGGCC TGCAGAGTCT CGGAAAAGTT 
GCCATTGCCC GGCGCATGCC ACCCCCAGCC AACCTTCCAA GCCTGAAAGC CGAGAACAAA 
GGCAATGACC CCAATGTCTC ACTAGTGCCG AAAGACGGAA CAGGATGGGC AAGCAAACAG 
GAGCAGTCCG ACCCCAAGAG TTCCGATGCC TCAACCGCTC AGCCGCCGGA ATCGCAGCCA 
CTGCCGGCTT CACAGACGCC TGCCTCCAAC CAGCCGAAAC GACCCCCAAC AGCCCCCGAG 
AACACTCCTT CGGTTCCAAG CGGGGTAAAG TCCTGGGCAC AAGCCAGTGT CACCCATGGA 
GCCCATGGAG ATGGTGGAAG GGCATCAAAC CTACTGTCGC GATTCTCTCG AGAGGAATTT 
CCGACCCTGC AGGCGGCTGG AGACCAGGAC AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA 
CAGTCGTCTG GGCCCGGACC AAGCCTCCGC CCCCAAAATT CTACAACTTG GAGGGACGGA 
GGTGGGCGTG GCCCTGATGA CCTGGAGGGC CCGGACTCCA AACTTCATCA TGGCCATGAT 
CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA CCCCAGTTCC CTCCCTACCG CGGGATGATG 
CCGCCCTTTA TGTATCCCCC ATATCTCCCA TTCCCTCCGC CCTATGGACC CCAGGGGCCT 
TATAGATACC CGACTCCTGA TGGGCCCAGC CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC 
TCAGGGCCAC CAATGCGCCT TGTAGAGCCT GTGGGTCGGC CTTCTATTCT TAAAGAGGAT 
AATCTTAAAG AGTTTGACCA ACTGGACCAG GAAAATGATG ATGGTTGGGC AGGGGCCCAT 
GAAGAGGTTG ACTATACTGA AAAGCTCAAA TTCAGCGATG AGGAAGATGG ACGAGACTCC 
GACGAGGAGG GAGCTGAGGG CCACAAGGAT TCCCAGTCAG CTGCTGCTGA GGAACCTGAG 
ACAGATGGCA AAAAGGGCAC CTCCCCTGGC AGCGAATTGC CCCCTCCCAA GACAGCCTGG 
ACAGAGAATG CTCGGCCTTC AGAGACAGAG CCAGCTCCTC CTACCCCAAA GCCTCCCCCA 
CCTCCACCTC ACCGGGGGCC AGTCGGGAAC TGGGGGCCCC CTGGGGACTA CCCAGATCGA 
GGGGGTCCTC CCTGCAAACC CCCAGCACCT GAGGATGAGG ATGAGGCATG GCGGCAGCGT 
CGGAAGCAAT CTTCTTCAGA GATCTCCCTG GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA 
GAGGAAGAGC GACGCATGCA GGAGGAGCGC CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT 
CTTGATGAAA AGTTTGGGGC ACCTGACAAG CGGCTCAAGG CAGAGCCTGC AGCCCCACCT 
GTTACCCCTG CTGCCCCAGC TCTACCACCT GTAGTCCCCA AAGAAATTCC TGCAGCTCCA 
GCTCTGCCAC CAACACCTAC CCCAACACCA GAGAAAGAAC CTGAAGAGCC GGCACAAGCC 
CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG GTGGCTCCAG TTCCCACTCT GGTGAGTGGG 
GGTGGTTGCA CTGCCAACAG CAACAGCAGT GGCAGCTTCG AAGCCAGCCC AGTGGAACCC 
CAACTGCCTT CAAAAGAGGG TCCTGAACCA CCAGAAGAGG TTCCTCCACC TACCACACCC 
CCAGCCCCAA AGATGGAACC CAAAGGTGAT GGGGTTGGTT CTACTCGCCA GCCCCCCAGT 
CAGGGCTTGG GCTACCCCAA ATACCAGAAG TCATTGCCTC CTCGCTTCCA GCGGCAGCAA 
CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG CAACAGCAGT GGCAGCAGCA GCAACAAGGC 
ACTGCCCCTC CTGCCCCAGT GCCCCCATCA CCACCACAAC CTGTGACCCT GGGAGCTGTA 
CCTGCCCCAC AAGCACCGCC CCCACCTCCC AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG 
CCCCCACCAA TGCCTCCAAT GAACTTTGAC CCCCGCTGGA TGATGATTCC TCCTTACGTG 
GACCCTCGGC TTCTCCAGGG CCGGCCCCCT CTGGACTTCT ACCCTCCTGG TGTGCATCCC 
TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC AGTGGGGGCT CAAGCTCAGA GCCATTTGAA 
CGCCATGCAC CCCCTCTGTT GCGGGAAAGG GGCACCCCAC CAGTGGACCC CAAGTTGGCC 
TGGGTTGGAG ATGTCTTTAC CACCACACCC ACTGATCCTC GTCCTCTTAC CTCACCACTG 
CGCCAGGCAG CAGATGAGGA GGAGAAGAGT ATGAGGAGCG AGACTCCTCC GGTACCCCCG 
CCCCCACCTT ATCTGGCTAA TTATCCAGGT TTTCCAGAGA ATGGAACTCC TGGGCCCCCA 
ATCTCGAGAT TCCCTCTGGA GGAATCTGCT CCCCCGGGAC CCCGCCCACT ACCCTGGCCT 
CCAGGCAATG ATGAAGCTGC CAAGATGCAG GCTCCACCGC CCAAGAAGGA ACCCTCGAAG 
GAGGAACCGC CACAGCTGTC CGGGCCAGAA GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA 
GGCCCACCAC CACCACGGAG AGAGAATCGT ACTGAGACTC GCTGGGGCCC CCGACCAGGC 
AGTTGTCGCC GTGGAATCCC TCCAGAGGAG CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT 
ATAAAGAAGC CACCACCACC TGTGAAAGTA GAGGAGCTGC CTCCTAAGTC CCTAGAACAG 
GGGGATGAGA CCCCCAAGGT CCCAAAGCCA GATGCACTCA AGACAGCTAA GGGCAAGGTG 
GGCCCCAAAG AAACCCCACC AGGTGGAAAC CTGTCTCCTG CACCTAGGCT TCGGCGGGAC 
TATTCCTATG AAAGAGTAGG TCCTACATCT TGCCGTGGTC GGGGCAGAGG CGAGTATTTT 
GCCAGAGGAA GGGGTTTTCG GGGGACCTAT GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT 
GAATTCCGAA GTTACCGAGA GTTCCGAGGA GACGATGGGC GTGGGGGTGG TTCAGGCGGA 
ACAAACCATC CTTCTGCTCC CCGAGGCCGC ACTGCCAGTG AGACACGAAG TGAGGGTTCA 
GAATATGAAG AAATCCCCAA GAGACGCCGC CAGCGTGGCT CTGAAACAGG CAGTGAGACT 
CATGAGAGTG ACCTGGCCCC CTCTGACAAG GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT 
GGTCAGGTCC CTCTTGCCCC CCCACAACCA GGAGCTCCTC CATCACCAGC CCCAGCCCGA 
TTCTCCACTG CCCGAGGTGG TCGTGTCTTC ACTCCAAGAG GAGTACCATC ACGCCGTGGC 
CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG 
GTTCCAAAGA AACCTCCAAC AGGTCCTTTG CCTCCAAGTA AAGAACCTTT GAAAGAGAAG 
CTGATCTCAG GGCCTCTGTC CCCCATGTCT CGAGCTGGCA ACATGGGTGT AGGCATGGAA 
GATGGGGAGC GACCCCGACG GAGGCGACAT GGCAGGGCTC AACAGCAGGA CAAACCACCT 
CGTTTCCGAA GGCTGAAACA AGAACGTGAA AACGCTGCCA GAGGAGCTGA TGGCAAGCCC 
CCTTCCCTAA CCCTTGCAGC CTCTACCCCT GGACCTGAGG AGACACTAAC AGCAGCCACA 
GTTCCCCCAC CCCCTCGCCG CACAGCTGCT AAGTCTCCTG ATCTGTCAAA CCAGAACTCA 
GACCAAGCCA ATGAGGAATG GGAGACTGCA TCAGAGAGCA GCGACTTTGC CAGTGAGCGC 
CGGGGTGACA AGGAGACTCC CCCAGCAGCA CTTATGACCT CTAAAGCTGT GGGGACTCCT 
GGGGCCAATG CAGGTGGGGC TGGACCAGGC ATTTCAGCCA TGTCTCGTGG AGACCTGAGC 
CAACGAGCCA AGGATTTGAG CAAGCGGAGC TTCTCAAGTC AGCGGCCAGG CATGGATCGT 
CAAAACCGGC GCCCAGGCAC TGGGGGCAAG ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT 
GGTGGTGCAG GCCCTGGGGG ACGGACCGGC CCAGGACGAG GGGATAAGAG GAGCTGGCCC 
TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG GAGCGACCTC CGGGACTTCC TCTGCCTCCG 
CCACCCCCCA GCAGTTCTGC TGTCTTCCGC CTGGACCAAG TTATCCATAG CAACCCTGCT 
GGAATCCAAC AGGCTCTGGC CCAACTTAGC AGCCGCCAAG GAAATGTAAC TGCACCAGGG 
GGGCACCCAA GGCCTAAGCC TGGGCCTCCC CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC 
CCAACCCGTT ATGACCCCCC AAGGGCCAGC AGCGCTATCA GTTCTGACCC CCACTTCGAG 
GAGCCAGGGC CGATGGTGAG GGGGGTGGGC GGGACTCCCC GGGACTCTGC CGGGGTTAAT 
CCCTTCCCCC CGAAACGGCG GGAGCGGCCT CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA 
ACTGTGCCAG CTTCTCATAG CTCTGGATTC TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT 
CAGGAAGAAT CCAGAGATTC AGGCACAGAG GCCCTAACTC CCCACATCTG GAATCGTTTA 
CATACTGCCA CAAGCCGAAA GAGTTACCAG CCTGGCTCCA TCGAGCCCTG GATGGAGCCC 
CTGAGTCCCT TTGAGGATGT GGCAGGCACA GAGATGAGTC AGTCTGACAG TGGAGTGGAC 
CTGAGTGGAG ATTCTCAGGT GTCATCAGGT CCCTGCAGTC AGCGAAGTTC CCCTGATGGA 
GGACTTAAGG GGTCAGCAGA GGGACCCCCC AAGCGGCCTG GAGGACCCTC ACCCCTGAAT 
GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT GAACCCTCTG AACCTCCTAG GAGACGGCCA 
CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT 
CCCATAGGCA CAGAACGATC ACAGCGCACA GATCGAGGCC CAGAGCCAGG CCCTCTCCGA 
CCAGCCCACC GACCGGGCTC TCAAGTTGAG TTTGGCACTA CCAACAAGGA CTCAGACTTG 
TGCCTAGTGG TCGGAGACAC TTTAAAAGGA GAAAAGGAGT TGGTGGCATC AGCCACAGAG 
GCCGTTCCCA TATCCAGAGA CTGGGAGCTG CTCCCTAGTG CTTCTACTTC AGCTGAGCCA 
CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT 
CCGTACCCTG AGGTCTTCTA TGGCAGCCCT GGACCTCCTA ATTCCCAGCA GGTCTCTGGG 
GGTGCACCCA TCGACTCTCA GTTACATCCC AACAGTGGAG GCTTCCGTCC TGGGACACCC 
TCATTGCACC AGTACAGGTC ACAGCCTCTG TATCTGCCCC CAGGTCCAGC CCCTCCCTCG 
GCACTGCTTT CTGGGGTAGC TCTCAAGGGC CAGTTTCTGG ATTTCTCAGC ACTGCAAGCC 
ACAGAGCTGG GGAAGTTGCC TGCTGGGGGA GTTCTCTACC CTCCGCCATC CTTCCTCTAC 
TCTGCAGCTT TCTGCCCCAG CCCTTTGCCT GACCCACCCT TGCTTCAGGT ACGCCAGGAT 
CTGCCATCCC CTTCAGATTT TTACTCTACT CCTCTGCAGC CTGGTGGCCA GAGTGGCTTC 
CTTCCTTCGG GAGCCCCTGC TCAGCAGATG CTTCTACCTG TGGTAGACTC ACAGCTGCCA 
GTGGTGAACT TTGGCTCCCT GCCACCAGCG CCACCCCCTG CTCCACCCCC CCTTTCTCTG 
TTACCTGTGG GTCCTGCTCT CCAGCCCCCC AACCTAGCTG TGCGGCCCCC ACCTGCTCCT 
GCTGCTCGGG TCTTACCTTC ACCCGCTAGA CCCTTCGCCC CTAGCTTGGG GCGAGCAGAG 
CTGCACCCAG TAGAACTAAA GCCTTTCCAG GATTACCGAA AGCTGAGCAG CAACCTTGGG 
GGTCCTGGGT CGTCACGGAC TCCCCCATCT GGAAGGCCCT TCTCTGGTCT CAATTCCCGT 
CTCAAGGCCC CACCTTCCAC CTACAGTGGA GTATTCCGAA CTCAGCGCAT TGACCTCTAC 
CAGCAGGCTT CCCCACCAGA TGCCCTGCGC TGGATGCCGA AGCCTTGGGA ACGGGCAGGG 
CCACCTTCTC GAGAAGGGCC TCCCCGAAGA GCAGAGGAGC CTGGGTCCCG GGGGGAGAAG 
GAGCCTGGAT TGCCCCCACC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105760.1
CDS	153..6599
Translation	MSDRSGPTAKGKDGKKYSSLNLFDTYKVAPRHGLQSLGKVAIARRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQSDPKSSDASTAQPPESQPLPASQTPASNQPKRPPTAPENTPSVPSGVKSWAQASVTHGAHGDGGRASNLLSRFSREEFPTLQAAGDQDKAAKERESAEQSSGPGPSLRPQNSTTWRDGGGRGPDDLEGPDSKLHHGHDPRGGLQPSGPPQFPPYRGMMPPFMYPPYLPFPPPYGPQGPYRYPTPDGPSRFPRVAGPRGSGPPMRLVEPVGRPSILKEDNLKEFDQLDQENDDGWAGAHEEVDYTEKLKFSDEEDGRDSDEEGAEGHKDSQSAAAEEPETDGKKGTSPGSELPPPKTAWTENARPSETEPAPPTPKPPPPPPHRGPVGNWGPPGDYPDRGGPPCKPPAPEDEDEAWRQRRKQSSSEISLAVERARRRREEEERRMQEERRAACAEKLKRLDEKFGAPDKRLKAEPAAPPVTPAAPALPPVVPKEIPAAPALPPTPTPTPEKEPEEPAQAPPVQAAPSPGVAPVPTLVSGGGCTANSNSSGSFEASPVEPQLPSKEGPEPPEEVPPPTTPPAPKMEPKGDGVGSTRQPPSQGLGYPKYQKSLPPRFQRQQQEQLLKQQQQQQQWQQQQQGTAPPAPVPPSPPQPVTLGAVPAPQAPPPPPKALYPGALGRPPPMPPMNFDPRWMMIPPYVDPRLLQGRPPLDFYPPGVHPSGLVPRERSDSGGSSSEPFERHAPPLLRERGTPPVDPKLAWVGDVFTTTPTDPRPLTSPLRQAADEEEKSMRSETPPVPPPPPYLANYPGFPENGTPGPPISRFPLEESAPPGPRPLPWPPGNDEAAKMQAPPPKKEPSKEEPPQLSGPEAGRKPARGGQGPPPPRRENRTETRWGPRPGSCRRGIPPEEPGVPPRRAGPIKKPPPPVKVEELPPKSLEQGDETPKVPKPDALKTAKGKVGPKETPPGGNLSPAPRLRRDYSYERVGPTSCRGRGRGEYFARGRGFRGTYGGRGRGARSREFRSYREFRGDDGRGGGSGGTNHPSAPRGRTASETRSEGSEYEEIPKRRRQRGSETGSETHESDLAPSDKEAPPPKEGVLGQVPLAPPQPGAPPSPAPARFSTARGGRVFTPRGVPSRRGRGGGRPPPVCSGWSPPAKSLVPKKPPTGPLPPSKEPLKEKLISGPLSPMSRAGNMGVGMEDGERPRRRRHGRAQQQDKPPRFRRLKQERENAARGADGKPPSLTLAASTPGPEETLTAATVPPPPRRTAAKSPDLSNQNSDQANEEWETASESSDFASERRGDKETPPAALMTSKAVGTPGANAGGAGPGISAMSRGDLSQRAKDLSKRSFSSQRPGMDRQNRRPGTGGKTGSGGGSSGGGGAGPGGRTGPGRGDKRSWPSPKNRSRPPEERPPGLPLPPPPPSSSAVFRLDQVIHSNPAGIQQALAQLSSRQGNVTAPGGHPRPKPGPPQAPQGSSPRPPTRYDPPRASSAISSDPHFEEPGPMVRGVGGTPRDSAGVNPFPPKRRERPPRKPELLQEETVPASHSSGFLGSKPEVPGPQEESRDSGTEALTPHIWNRLHTATSRKSYQPGSIEPWMEPLSPFEDVAGTEMSQSDSGVDLSGDSQVSSGPCSQRSSPDGGLKGSAEGPPKRPGGPSPLNAVPGESASGSEPSEPPRRRPPASHEGERKELPREQPLPPGPIGTERSQRTDRGPEPGPLRPAHRPGSQVEFGTTNKDSDLCLVVGDTLKGEKELVASATEAVPISRDWELLPSASTSAEPQPKSLGSGQCVPEPSPSGQRPYPEVFYGSPGPPNSQQVSGGAPIDSQLHPNSGGFRPGTPSLHQYRSQPLYLPPGPAPPSALLSGVALKGQFLDFSALQATELGKLPAGGVLYPPPSFLYSAAFCPSPLPDPPLLQVRQDLPSPSDFYSTPLQPGGQSGFLPSGAPAQQMLLPVVDSQLPVVNFGSLPPAPPPAPPPLSLLPVGPALQPPNLAVRPPPAPAARVLPSPARPFAPSLGRAELHPVELKPFQDYRKLSSNLGGPGSSRTPPSGRPFSGLNSRLKAPPSTYSGVFRTQRIDLYQQASPPDALRWMPKPWERAGPPSREGPPRRAEEPGSRGEKEPGLPPPR

Target ORF information:

RefSeq Version	XM_030249900.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249900.1

ORF Insert Sequence:

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGTTGCCCCT CGACATGGCC TGCAGAGTCT CGGAAAAGTT 
GCCATTGCCC GGCGCATGCC ACCCCCAGCC AACCTTCCAA GCCTGAAAGC CGAGAACAAA 
GGCAATGACC CCAATGTCTC ACTAGTGCCG AAAGACGGAA CAGGATGGGC AAGCAAACAG 
GAGCAGTCCG ACCCCAAGAG TTCCGATGCC TCAACCGCTC AGCCGCCGGA ATCGCAGCCA 
CTGCCGGCTT CACAGACGCC TGCCTCCAAC CAGCCGAAAC GACCCCCAAC AGCCCCCGAG 
AACACTCCTT CGGTTCCAAG CGGGGTAAAG TCCTGGGCAC AAGCCAGTGT CACCCATGGA 
GCCCATGGAG ATGGTGGAAG GGCATCAAAC CTACTGTCGC GATTCTCTCG AGAGGAATTT 
CCGACCCTGC AGGCGGCTGG AGACCAGGAC AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA 
CAGTCGTCTG GGCCCGGACC AAGCCTCCGC CCCCAAAATT CTACAACTTG GAGGGACGGA 
GGTGGGCGTG GCCCTGATGA CCTGGAGGGC CCGGACTCCA AACTTCATCA TGGCCATGAT 
CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA CCCCAGTTCC CTCCCTACCG CGGGATGATG 
CCGCCCTTTA TGTATCCCCC ATATCTCCCA TTCCCTCCGC CCTATGGACC CCAGGGGCCT 
TATAGATACC CGACTCCTGA TGGGCCCAGC CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC 
TCAGGGCCAC CAATGCGCCT TGTAGAGCCT GTGGGTCGGC CTTCTATTCT TAAAGAGGAT 
AATCTTAAAG AGTTTGACCA ACTGGACCAG GAAAATGATG ATGGTTGGGC AGGGGCCCAT 
GAAGAGGTTG ACTATACTGA AAAGCTCAAA TTCAGCGATG AGGAAGATGG ACGAGACTCC 
GACGAGGAGG GAGCTGAGGG CCACAAGGAT TCCCAGTCAG CTGCTGCTGA GGAACCTGAG 
ACAGATGGCA AAAAGGGCAC CTCCCCTGGC AGCGAATTGC CCCCTCCCAA GACAGCCTGG 
ACAGAGAATG CTCGGCCTTC AGAGACAGAG CCAGCTCCTC CTACCCCAAA GCCTCCCCCA 
CCTCCACCTC ACCGGGGGCC AGTCGGGAAC TGGGGGCCCC CTGGGGACTA CCCAGATCGA 
GGGGGTCCTC CCTGCAAACC CCCAGCACCT GAGGATGAGG ATGAGGCATG GCGGCAGCGT 
CGGAAGCAAT CTTCTTCAGA GATCTCCCTG GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA 
GAGGAAGAGC GACGCATGCA GGAGGAGCGC CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT 
CTTGATGAAA AGTTTGGGGC ACCTGACAAG CGGCTCAAGG CAGAGCCTGC AGCCCCACCT 
GTTACCCCTG CTGCCCCAGC TCTACCACCT GTAGTCCCCA AAGAAATTCC TGCAGCTCCA 
GCTCTGCCAC CAACACCTAC CCCAACACCA GAGAAAGAAC CTGAAGAGCC GGCACAAGCC 
CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG GTGGCTCCAG TTCCCACTCT GGTGAGTGGG 
GGTGGTTGCA CTGCCAACAG CAACAGCAGT GGCAGCTTCG AAGCCAGCCC AGTGGAACCC 
CAACTGCCTT CAAAAGAGGG TCCTGAACCA CCAGAAGAGG TTCCTCCACC TACCACACCC 
CCAGCCCCAA AGATGGAACC CAAAGGTGAT GGGGTTGGTT CTACTCGCCA GCCCCCCAGT 
CAGGGCTTGG GCTACCCCAA ATACCAGAAG TCATTGCCTC CTCGCTTCCA GCGGCAGCAA 
CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG CAACAGCAGT GGCAGCAGCA GCAACAAGGC 
ACTGCCCCTC CTGCCCCAGT GCCCCCATCA CCACCACAAC CTGTGACCCT GGGAGCTGTA 
CCTGCCCCAC AAGCACCGCC CCCACCTCCC AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG 
CCCCCACCAA TGCCTCCAAT GAACTTTGAC CCCCGCTGGA TGATGATTCC TCCTTACGTG 
GACCCTCGGC TTCTCCAGGG CCGGCCCCCT CTGGACTTCT ACCCTCCTGG TGTGCATCCC 
TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC AGTGGGGGCT CAAGCTCAGA GCCATTTGAA 
CGCCATGCAC CCCCTCTGTT GCGGGAAAGG GGCACCCCAC CAGTGGACCC CAAGTTGGCC 
TGGGTTGGAG ATGTCTTTAC CACCACACCC ACTGATCCTC GTCCTCTTAC CTCACCACTG 
CGCCAGGCAG CAGATGAGGA GGAGAAGAGT ATGAGGAGCG AGACTCCTCC GGTACCCCCG 
CCCCCACCTT ATCTGGCTAA TTATCCAGGT TTTCCAGAGA ATGGAACTCC TGGGCCCCCA 
ATCTCGAGAT TCCCTCTGGA GGAATCTGCT CCCCCGGGAC CCCGCCCACT ACCCTGGCCT 
CCAGGCAATG ATGAAGCTGC CAAGATGCAG GCTCCACCGC CCAAGAAGGA ACCCTCGAAG 
GAGGAACCGC CACAGCTGTC CGGGCCAGAA GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA 
GGCCCACCAC CACCACGGAG AGAGAATCGT ACTGAGACTC GCTGGGGCCC CCGACCAGGC 
AGTTGTCGCC GTGGAATCCC TCCAGAGGAG CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT 
ATAAAGAAGC CACCACCACC TGTGAAAGTA GAGGAGCTGC CTCCTAAGTC CCTAGAACAG 
GGGGATGAGA CCCCCAAGGT CCCAAAGCCA GATGCACTCA AGACAGCTAA GGGCAAGGTG 
GGCCCCAAAG AAACCCCACC AGGTGGAAAC CTGTCTCCTG CACCTAGGCT TCGGCGGGAC 
TATTCCTATG AAAGAGTAGG TCCTACATCT TGCCGTGGTC GGGGCAGAGG CGAGTATTTT 
GCCAGAGGAA GGGGTTTTCG GGGGACCTAT GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT 
GAATTCCGAA GTTACCGAGA GTTCCGAGGA GACGATGGGC GTGGGGGTGG TTCAGGCGGA 
ACAAACCATC CTTCTGCTCC CCGAGGCCGC ACTGCCAGTG AGACACGAAG TGAGGGTTCA 
GAATATGAAG AAATCCCCAA GAGACGCCGC CAGCGTGGCT CTGAAACAGG CAGTGAGACT 
CATGAGAGTG ACCTGGCCCC CTCTGACAAG GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT 
GGTCAGGTCC CTCTTGCCCC CCCACAACCA GGAGCTCCTC CATCACCAGC CCCAGCCCGA 
TTCTCCACTG CCCGAGGTGG TCGTGTCTTC ACTCCAAGAG GAGTACCATC ACGCCGTGGC 
CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG 
GTTCCAAAGA AACCTCCAAC AGGTCCTTTG CCTCCAAGTA AAGAACCTTT GAAAGAGAAG 
CTGATCTCAG GGCCTCTGTC CCCCATGTCT CGAGCTGGCA ACATGGGTGT AGGCATGGAA 
GATGGGGAGC GACCCCGACG GAGGCGACAT GGCAGGGCTC AACAGCAGGA CAAACCACCT 
CGTTTCCGAA GGCTGAAACA AGAACGTGAA AACGCTGCCA GAGGAGCTGA TGGCAAGCCC 
CCTTCCCTAA CCCTTGCAGC CTCTACCCCT GGACCTGAGG AGACACTAAC AGCAGCCACA 
GTTCCCCCAC CCCCTCGCCG CACAGCTGCT AAGTCTCCTG ATCTGTCAAA CCAGAACTCA 
GACCAAGCCA ATGAGGAATG GGAGACTGCA TCAGAGAGCA GCGACTTTGC CAGTGAGCGC 
CGGGGTGACA AGGAGACTCC CCCAGCAGCA CTTATGACCT CTAAAGCTGT GGGGACTCCT 
GGGGCCAATG CAGGTGGGGC TGGACCAGGC ATTTCAGCCA TGTCTCGTGG AGACCTGAGC 
CAACGAGCCA AGGATTTGAG CAAGCGGAGC TTCTCAAGTC AGCGGCCAGG CATGGATCGT 
CAAAACCGGC GCCCAGGCAC TGGGGGCAAG ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT 
GGTGGTGCAG GCCCTGGGGG ACGGACCGGC CCAGGACGAG GGGATAAGAG GAGCTGGCCC 
TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG GAGCGACCTC CGGGACTTCC TCTGCCTCCG 
CCACCCCCCA GCAGTTCTGC TGTCTTCCGC CTGGACCAAG TTATCCATAG CAACCCTGCT 
GGAATCCAAC AGGCTCTGGC CCAACTTAGC AGCCGCCAAG GAAATGTAAC TGCACCAGGG 
GGGCACCCAA GGCCTAAGCC TGGGCCTCCC CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC 
CCAACCCGTT ATGACCCCCC AAGGGCCAGC AGCGCTATCA GTTCTGACCC CCACTTCGAG 
GAGCCAGGGC CGATGGTGAG GGGGGTGGGC GGGACTCCCC GGGACTCTGC CGGGGTTAAT 
CCCTTCCCCC CGAAACGGCG GGAGCGGCCT CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA 
ACTGTGCCAG CTTCTCATAG CTCTGGATTC TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT 
CAGGAAGAAT CCAGAGATTC AGGCACAGAG GCCCTAACTC CCCACATCTG GAATCGTTTA 
CATACTGCCA CAAGCCGAAA GAGTTACCAG CCTGGCTCCA TCGAGCCCTG GATGGAGCCC 
CTGAGTCCCT TTGAGGATGT GGCAGGCACA GAGATGAGTC AGTCTGACAG TGGAGTGGAC 
CTGAGTGGAG ATTCTCAGGT GTCATCAGGT CCCTGCAGTC AGCGAAGTTC CCCTGATGGA 
GGACTTAAGG GGTCAGCAGA GGGACCCCCC AAGCGGCCTG GAGGACCCTC ACCCCTGAAT 
GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT GAACCCTCTG AACCTCCTAG GAGACGGCCA 
CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT 
CCCATAGGCA CAGAACGATC ACAGCGCACA GATCGAGGCC CAGAGCCAGG CCCTCTCCGA 
CCAGCCCACC GACCGGGCTC TCAAGTTGAG TTTGGCACTA CCAACAAGGA CTCAGACTTG 
TGCCTAGTGG TCGGAGACAC TTTAAAAGGA GAAAAGGAGT TGGTGGCATC AGCCACAGAG 
GCCGTTCCCA TATCCAGAGA CTGGGAGCTG CTCCCTAGTG CTTCTACTTC AGCTGAGCCA 
CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT 
CCGTACCCTG AGGTCTTCTA TGGCAGCCCT GGACCTCCTA ATTCCCAGCA GGTCTCTGGG 
GGTGCACCCA TCGACTCTCA GTTACATCCC AACAGTGGAG GCTTCCGTCC TGGGACACCC 
TCATTGCACC AGTACAGGTC ACAGCCTCTG TATCTGCCCC CAGGTCCAGC CCCTCCCTCG 
GCACTGCTTT CTGGGGTAGC TCTCAAGGGC CAGTTTCTGG ATTTCTCAGC ACTGCAAGCC 
ACAGAGCTGG GGAAGTTGCC TGCTGGGGGA GTTCTCTACC CTCCGCCATC CTTCCTCTAC 
TCTGCAGCTT TCTGCCCCAG CCCTTTGCCT GACCCACCCT TGCTTCAGGT ACGCCAGGAT 
CTGCCATCCC CTTCAGATTT TTACTCTACT CCTCTGCAGC CTGGTGGCCA GAGTGGCTTC 
CTTCCTTCGG GAGCCCCTGC TCAGCAGATG CTTCTACCTG TGGTAGACTC ACAGCTGCCA 
GTGGTGAACT TTGGCTCCCT GCCACCAGCG CCACCCCCTG CTCCACCCCC CCTTTCTCTG 
TTACCTGTGG GTCCTGCTCT CCAGCCCCCC AACCTAGCTG TGCGGCCCCC ACCTGCTCCT 
GCTGCTCGGG TCTTACCTTC ACCCGCTAGA CCCTTCGCCC CTAGCTTGGG GCGAGCAGAG 
CTGCACCCAG TAGAACTAAA GCCTTTCCAG GATTACCGAA AGCTGAGCAG CAACCTTGGG 
GGTCCTGGGT CGTCACGGAC TCCCCCATCT GGAAGGCCCT TCTCTGGTCT CAATTCCCGT 
CTCAAGGCCC CACCTTCCAC CTACAGTGGA GTATTCCGAA CTCAGCGCAT TGACCTCTAC 
CAGCAGGCTT CCCCACCAGA TGCCCTGCGC TGGATGCCGA AGCCTTGGGA ACGGGCAGGG 
CCACCTTCTC GAGAAGGGCC TCCCCGAAGA GCAGAGGAGC CTGGGTCCCG GGGGGAGAAG 
GAGCCTGGAT TGCCCCCACC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prrc2a ( XM_006524621.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524684.1 Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X4, mRNA.

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CloneID	OMu53832
Clone ID Related Accession (Same CDS sequence)	XM_006524621.3 , XM_030249900.1 , XM_006524621.4
Accession Version	XM_006524621.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein PRRC2A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524621.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGTTGCCCCT CGACATGGCC TGCAGAGTCT CGGAAAAGTT 
GCCATTGCCC GGCGCATGCC ACCCCCAGCC AACCTTCCAA GCCTGAAAGC CGAGAACAAA 
GGCAATGACC CCAATGTCTC ACTAGTGCCG AAAGACGGAA CAGGATGGGC AAGCAAACAG 
GAGCAGTCCG ACCCCAAGAG TTCCGATGCC TCAACCGCTC AGCCGCCGGA ATCGCAGCCA 
CTGCCGGCTT CACAGACGCC TGCCTCCAAC CAGCCGAAAC GACCCCCAAC AGCCCCCGAG 
AACACTCCTT CGGTTCCAAG CGGGGTAAAG TCCTGGGCAC AAGCCAGTGT CACCCATGGA 
GCCCATGGAG ATGGTGGAAG GGCATCAAAC CTACTGTCGC GATTCTCTCG AGAGGAATTT 
CCGACCCTGC AGGCGGCTGG AGACCAGGAC AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA 
CAGTCGTCTG GGCCCGGACC AAGCCTCCGC CCCCAAAATT CTACAACTTG GAGGGACGGA 
GGTGGGCGTG GCCCTGATGA CCTGGAGGGC CCGGACTCCA AACTTCATCA TGGCCATGAT 
CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA CCCCAGTTCC CTCCCTACCG CGGGATGATG 
CCGCCCTTTA TGTATCCCCC ATATCTCCCA TTCCCTCCGC CCTATGGACC CCAGGGGCCT 
TATAGATACC CGACTCCTGA TGGGCCCAGC CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC 
TCAGGGCCAC CAATGCGCCT TGTAGAGCCT GTGGGTCGGC CTTCTATTCT TAAAGAGGAT 
AATCTTAAAG AGTTTGACCA ACTGGACCAG GAAAATGATG ATGGTTGGGC AGGGGCCCAT 
GAAGAGGTTG ACTATACTGA AAAGCTCAAA TTCAGCGATG AGGAAGATGG ACGAGACTCC 
GACGAGGAGG GAGCTGAGGG CCACAAGGAT TCCCAGTCAG CTGCTGCTGA GGAACCTGAG 
ACAGATGGCA AAAAGGGCAC CTCCCCTGGC AGCGAATTGC CCCCTCCCAA GACAGCCTGG 
ACAGAGAATG CTCGGCCTTC AGAGACAGAG CCAGCTCCTC CTACCCCAAA GCCTCCCCCA 
CCTCCACCTC ACCGGGGGCC AGTCGGGAAC TGGGGGCCCC CTGGGGACTA CCCAGATCGA 
GGGGGTCCTC CCTGCAAACC CCCAGCACCT GAGGATGAGG ATGAGGCATG GCGGCAGCGT 
CGGAAGCAAT CTTCTTCAGA GATCTCCCTG GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA 
GAGGAAGAGC GACGCATGCA GGAGGAGCGC CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT 
CTTGATGAAA AGTTTGGGGC ACCTGACAAG CGGCTCAAGG CAGAGCCTGC AGCCCCACCT 
GTTACCCCTG CTGCCCCAGC TCTACCACCT GTAGTCCCCA AAGAAATTCC TGCAGCTCCA 
GCTCTGCCAC CAACACCTAC CCCAACACCA GAGAAAGAAC CTGAAGAGCC GGCACAAGCC 
CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG GTGGCTCCAG TTCCCACTCT GGTGAGTGGG 
GGTGGTTGCA CTGCCAACAG CAACAGCAGT GGCAGCTTCG AAGCCAGCCC AGTGGAACCC 
CAACTGCCTT CAAAAGAGGG TCCTGAACCA CCAGAAGAGG TTCCTCCACC TACCACACCC 
CCAGCCCCAA AGATGGAACC CAAAGGTGAT GGGGTTGGTT CTACTCGCCA GCCCCCCAGT 
CAGGGCTTGG GCTACCCCAA ATACCAGAAG TCATTGCCTC CTCGCTTCCA GCGGCAGCAA 
CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG CAACAGCAGT GGCAGCAGCA GCAACAAGGC 
ACTGCCCCTC CTGCCCCAGT GCCCCCATCA CCACCACAAC CTGTGACCCT GGGAGCTGTA 
CCTGCCCCAC AAGCACCGCC CCCACCTCCC AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG 
CCCCCACCAA TGCCTCCAAT GAACTTTGAC CCCCGCTGGA TGATGATTCC TCCTTACGTG 
GACCCTCGGC TTCTCCAGGG CCGGCCCCCT CTGGACTTCT ACCCTCCTGG TGTGCATCCC 
TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC AGTGGGGGCT CAAGCTCAGA GCCATTTGAA 
CGCCATGCAC CCCCTCTGTT GCGGGAAAGG GGCACCCCAC CAGTGGACCC CAAGTTGGCC 
TGGGTTGGAG ATGTCTTTAC CACCACACCC ACTGATCCTC GTCCTCTTAC CTCACCACTG 
CGCCAGGCAG CAGATGAGGA GGAGAAGAGT ATGAGGAGCG AGACTCCTCC GGTACCCCCG 
CCCCCACCTT ATCTGGCTAA TTATCCAGGT TTTCCAGAGA ATGGAACTCC TGGGCCCCCA 
ATCTCGAGAT TCCCTCTGGA GGAATCTGCT CCCCCGGGAC CCCGCCCACT ACCCTGGCCT 
CCAGGCAATG ATGAAGCTGC CAAGATGCAG GCTCCACCGC CCAAGAAGGA ACCCTCGAAG 
GAGGAACCGC CACAGCTGTC CGGGCCAGAA GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA 
GGCCCACCAC CACCACGGAG AGAGAATCGT ACTGAGACTC GCTGGGGCCC CCGACCAGGC 
AGTTGTCGCC GTGGAATCCC TCCAGAGGAG CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT 
ATAAAGAAGC CACCACCACC TGTGAAAGTA GAGGAGCTGC CTCCTAAGTC CCTAGAACAG 
GGGGATGAGA CCCCCAAGGT CCCAAAGCCA GATGCACTCA AGACAGCTAA GGGCAAGGTG 
GGCCCCAAAG AAACCCCACC AGGTGGAAAC CTGTCTCCTG CACCTAGGCT TCGGCGGGAC 
TATTCCTATG AAAGAGTAGG TCCTACATCT TGCCGTGGTC GGGGCAGAGG CGAGTATTTT 
GCCAGAGGAA GGGGTTTTCG GGGGACCTAT GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT 
GAATTCCGAA GTTACCGAGA GTTCCGAGGA GACGATGGGC GTGGGGGTGG TTCAGGCGGA 
ACAAACCATC CTTCTGCTCC CCGAGGCCGC ACTGCCAGTG AGACACGAAG TGAGGGTTCA 
GAATATGAAG AAATCCCCAA GAGACGCCGC CAGCGTGGCT CTGAAACAGG CAGTGAGACT 
CATGAGAGTG ACCTGGCCCC CTCTGACAAG GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT 
GGTCAGGTCC CTCTTGCCCC CCCACAACCA GGAGCTCCTC CATCACCAGC CCCAGCCCGA 
TTCTCCACTG CCCGAGGTGG TCGTGTCTTC ACTCCAAGAG GAGTACCATC ACGCCGTGGC 
CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG 
GTTCCAAAGA AACCTCCAAC AGGTCCTTTG CCTCCAAGTA AAGAACCTTT GAAAGAGAAG 
CTGATCTCAG GGCCTCTGTC CCCCATGTCT CGAGCTGGCA ACATGGGTGT AGGCATGGAA 
GATGGGGAGC GACCCCGACG GAGGCGACAT GGCAGGGCTC AACAGCAGGA CAAACCACCT 
CGTTTCCGAA GGCTGAAACA AGAACGTGAA AACGCTGCCA GAGGAGCTGA TGGCAAGCCC 
CCTTCCCTAA CCCTTGCAGC CTCTACCCCT GGACCTGAGG AGACACTAAC AGCAGCCACA 
GTTCCCCCAC CCCCTCGCCG CACAGCTGCT AAGTCTCCTG ATCTGTCAAA CCAGAACTCA 
GACCAAGCCA ATGAGGAATG GGAGACTGCA TCAGAGAGCA GCGACTTTGC CAGTGAGCGC 
CGGGGTGACA AGGAGACTCC CCCAGCAGCA CTTATGACCT CTAAAGCTGT GGGGACTCCT 
GGGGCCAATG CAGGTGGGGC TGGACCAGGC ATTTCAGCCA TGTCTCGTGG AGACCTGAGC 
CAACGAGCCA AGGATTTGAG CAAGCGGAGC TTCTCAAGTC AGCGGCCAGG CATGGATCGT 
CAAAACCGGC GCCCAGGCAC TGGGGGCAAG ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT 
GGTGGTGCAG GCCCTGGGGG ACGGACCGGC CCAGGACGAG GGGATAAGAG GAGCTGGCCC 
TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG GAGCGACCTC CGGGACTTCC TCTGCCTCCG 
CCACCCCCCA GCAGTTCTGC TGTCTTCCGC CTGGACCAAG TTATCCATAG CAACCCTGCT 
GGAATCCAAC AGGCTCTGGC CCAACTTAGC AGCCGCCAAG GAAATGTAAC TGCACCAGGG 
GGGCACCCAA GGCCTAAGCC TGGGCCTCCC CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC 
CCAACCCGTT ATGACCCCCC AAGGGCCAGC AGCGCTATCA GTTCTGACCC CCACTTCGAG 
GAGCCAGGGC CGATGGTGAG GGGGGTGGGC GGGACTCCCC GGGACTCTGC CGGGGTTAAT 
CCCTTCCCCC CGAAACGGCG GGAGCGGCCT CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA 
ACTGTGCCAG CTTCTCATAG CTCTGGATTC TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT 
CAGGAAGAAT CCAGAGATTC AGGCACAGAG GCCCTAACTC CCCACATCTG GAATCGTTTA 
CATACTGCCA CAAGCCGAAA GAGTTACCAG CCTGGCTCCA TCGAGCCCTG GATGGAGCCC 
CTGAGTCCCT TTGAGGATGT GGCAGGCACA GAGATGAGTC AGTCTGACAG TGGAGTGGAC 
CTGAGTGGAG ATTCTCAGGT GTCATCAGGT CCCTGCAGTC AGCGAAGTTC CCCTGATGGA 
GGACTTAAGG GGTCAGCAGA GGGACCCCCC AAGCGGCCTG GAGGACCCTC ACCCCTGAAT 
GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT GAACCCTCTG AACCTCCTAG GAGACGGCCA 
CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT 
CCCATAGGCA CAGAACGATC ACAGCGCACA GATCGAGGCC CAGAGCCAGG CCCTCTCCGA 
CCAGCCCACC GACCGGGCTC TCAAGTTGAG TTTGGCACTA CCAACAAGGA CTCAGACTTG 
TGCCTAGTGG TCGGAGACAC TTTAAAAGGA GAAAAGGAGT TGGTGGCATC AGCCACAGAG 
GCCGTTCCCA TATCCAGAGA CTGGGAGCTG CTCCCTAGTG CTTCTACTTC AGCTGAGCCA 
CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT 
CCGTACCCTG AGGTCTTCTA TGGCAGCCCT GGACCTCCTA ATTCCCAGCA GGTCTCTGGG 
GGTGCACCCA TCGACTCTCA GTTACATCCC AACAGTGGAG GCTTCCGTCC TGGGACACCC 
TCATTGCACC AGTACAGGTC ACAGCCTCTG TATCTGCCCC CAGGTCCAGC CCCTCCCTCG 
GCACTGCTTT CTGGGGTAGC TCTCAAGGGC CAGTTTCTGG ATTTCTCAGC ACTGCAAGCC 
ACAGAGCTGG GGAAGTTGCC TGCTGGGGGA GTTCTCTACC CTCCGCCATC CTTCCTCTAC 
TCTGCAGCTT TCTGCCCCAG CCCTTTGCCT GACCCACCCT TGCTTCAGGT ACGCCAGGAT 
CTGCCATCCC CTTCAGATTT TTACTCTACT CCTCTGCAGC CTGGTGGCCA GAGTGGCTTC 
CTTCCTTCGG GAGCCCCTGC TCAGCAGATG CTTCTACCTG TGGTAGACTC ACAGCTGCCA 
GTGGTGAACT TTGGCTCCCT GCCACCAGCG CCACCCCCTG CTCCACCCCC CCTTTCTCTG 
TTACCTGTGG GTCCTGCTCT CCAGCCCCCC AACCTAGCTG TGCGGCCCCC ACCTGCTCCT 
GCTGCTCGGG TCTTACCTTC ACCCGCTAGA CCCTTCGCCC CTAGCTTGGG GCGAGCAGAG 
CTGCACCCAG TAGAACTAAA GCCTTTCCAG GATTACCGAA AGCTGAGCAG CAACCTTGGG 
GGTCCTGGGT CGTCACGGAC TCCCCCATCT GGAAGGCCCT TCTCTGGTCT CAATTCCCGT 
CTCAAGGCCC CACCTTCCAC CTACAGTGGA GTATTCCGAA CTCAGCGCAT TGACCTCTAC 
CAGCAGGCTT CCCCACCAGA TGCCCTGCGC TGGATGCCGA AGCCTTGGGA ACGGGCAGGG 
CCACCTTCTC GAGAAGGGCC TCCCCGAAGA GCAGAGGAGC CTGGGTCCCG GGGGGAGAAG 
GAGCCTGGAT TGCCCCCACC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524684.1
CDS	365..6811
Translation	MSDRSGPTAKGKDGKKYSSLNLFDTYKVAPRHGLQSLGKVAIARRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQSDPKSSDASTAQPPESQPLPASQTPASNQPKRPPTAPENTPSVPSGVKSWAQASVTHGAHGDGGRASNLLSRFSREEFPTLQAAGDQDKAAKERESAEQSSGPGPSLRPQNSTTWRDGGGRGPDDLEGPDSKLHHGHDPRGGLQPSGPPQFPPYRGMMPPFMYPPYLPFPPPYGPQGPYRYPTPDGPSRFPRVAGPRGSGPPMRLVEPVGRPSILKEDNLKEFDQLDQENDDGWAGAHEEVDYTEKLKFSDEEDGRDSDEEGAEGHKDSQSAAAEEPETDGKKGTSPGSELPPPKTAWTENARPSETEPAPPTPKPPPPPPHRGPVGNWGPPGDYPDRGGPPCKPPAPEDEDEAWRQRRKQSSSEISLAVERARRRREEEERRMQEERRAACAEKLKRLDEKFGAPDKRLKAEPAAPPVTPAAPALPPVVPKEIPAAPALPPTPTPTPEKEPEEPAQAPPVQAAPSPGVAPVPTLVSGGGCTANSNSSGSFEASPVEPQLPSKEGPEPPEEVPPPTTPPAPKMEPKGDGVGSTRQPPSQGLGYPKYQKSLPPRFQRQQQEQLLKQQQQQQQWQQQQQGTAPPAPVPPSPPQPVTLGAVPAPQAPPPPPKALYPGALGRPPPMPPMNFDPRWMMIPPYVDPRLLQGRPPLDFYPPGVHPSGLVPRERSDSGGSSSEPFERHAPPLLRERGTPPVDPKLAWVGDVFTTTPTDPRPLTSPLRQAADEEEKSMRSETPPVPPPPPYLANYPGFPENGTPGPPISRFPLEESAPPGPRPLPWPPGNDEAAKMQAPPPKKEPSKEEPPQLSGPEAGRKPARGGQGPPPPRRENRTETRWGPRPGSCRRGIPPEEPGVPPRRAGPIKKPPPPVKVEELPPKSLEQGDETPKVPKPDALKTAKGKVGPKETPPGGNLSPAPRLRRDYSYERVGPTSCRGRGRGEYFARGRGFRGTYGGRGRGARSREFRSYREFRGDDGRGGGSGGTNHPSAPRGRTASETRSEGSEYEEIPKRRRQRGSETGSETHESDLAPSDKEAPPPKEGVLGQVPLAPPQPGAPPSPAPARFSTARGGRVFTPRGVPSRRGRGGGRPPPVCSGWSPPAKSLVPKKPPTGPLPPSKEPLKEKLISGPLSPMSRAGNMGVGMEDGERPRRRRHGRAQQQDKPPRFRRLKQERENAARGADGKPPSLTLAASTPGPEETLTAATVPPPPRRTAAKSPDLSNQNSDQANEEWETASESSDFASERRGDKETPPAALMTSKAVGTPGANAGGAGPGISAMSRGDLSQRAKDLSKRSFSSQRPGMDRQNRRPGTGGKTGSGGGSSGGGGAGPGGRTGPGRGDKRSWPSPKNRSRPPEERPPGLPLPPPPPSSSAVFRLDQVIHSNPAGIQQALAQLSSRQGNVTAPGGHPRPKPGPPQAPQGSSPRPPTRYDPPRASSAISSDPHFEEPGPMVRGVGGTPRDSAGVNPFPPKRRERPPRKPELLQEETVPASHSSGFLGSKPEVPGPQEESRDSGTEALTPHIWNRLHTATSRKSYQPGSIEPWMEPLSPFEDVAGTEMSQSDSGVDLSGDSQVSSGPCSQRSSPDGGLKGSAEGPPKRPGGPSPLNAVPGESASGSEPSEPPRRRPPASHEGERKELPREQPLPPGPIGTERSQRTDRGPEPGPLRPAHRPGSQVEFGTTNKDSDLCLVVGDTLKGEKELVASATEAVPISRDWELLPSASTSAEPQPKSLGSGQCVPEPSPSGQRPYPEVFYGSPGPPNSQQVSGGAPIDSQLHPNSGGFRPGTPSLHQYRSQPLYLPPGPAPPSALLSGVALKGQFLDFSALQATELGKLPAGGVLYPPPSFLYSAAFCPSPLPDPPLLQVRQDLPSPSDFYSTPLQPGGQSGFLPSGAPAQQMLLPVVDSQLPVVNFGSLPPAPPPAPPPLSLLPVGPALQPPNLAVRPPPAPAARVLPSPARPFAPSLGRAELHPVELKPFQDYRKLSSNLGGPGSSRTPPSGRPFSGLNSRLKAPPSTYSGVFRTQRIDLYQQASPPDALRWMPKPWERAGPPSREGPPRRAEEPGSRGEKEPGLPPPR

Target ORF information:

RefSeq Version	XM_006524621.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524621.4

ORF Insert Sequence:

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGTTGCCCCT CGACATGGCC TGCAGAGTCT CGGAAAAGTT 
GCCATTGCCC GGCGCATGCC ACCCCCAGCC AACCTTCCAA GCCTGAAAGC CGAGAACAAA 
GGCAATGACC CCAATGTCTC ACTAGTGCCG AAAGACGGAA CAGGATGGGC AAGCAAACAG 
GAGCAGTCCG ACCCCAAGAG TTCCGATGCC TCAACCGCTC AGCCGCCGGA ATCGCAGCCA 
CTGCCGGCTT CACAGACGCC TGCCTCCAAC CAGCCGAAAC GACCCCCAAC AGCCCCCGAG 
AACACTCCTT CGGTTCCAAG CGGGGTAAAG TCCTGGGCAC AAGCCAGTGT CACCCATGGA 
GCCCATGGAG ATGGTGGAAG GGCATCAAAC CTACTGTCGC GATTCTCTCG AGAGGAATTT 
CCGACCCTGC AGGCGGCTGG AGACCAGGAC AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA 
CAGTCGTCTG GGCCCGGACC AAGCCTCCGC CCCCAAAATT CTACAACTTG GAGGGACGGA 
GGTGGGCGTG GCCCTGATGA CCTGGAGGGC CCGGACTCCA AACTTCATCA TGGCCATGAT 
CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA CCCCAGTTCC CTCCCTACCG CGGGATGATG 
CCGCCCTTTA TGTATCCCCC ATATCTCCCA TTCCCTCCGC CCTATGGACC CCAGGGGCCT 
TATAGATACC CGACTCCTGA TGGGCCCAGC CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC 
TCAGGGCCAC CAATGCGCCT TGTAGAGCCT GTGGGTCGGC CTTCTATTCT TAAAGAGGAT 
AATCTTAAAG AGTTTGACCA ACTGGACCAG GAAAATGATG ATGGTTGGGC AGGGGCCCAT 
GAAGAGGTTG ACTATACTGA AAAGCTCAAA TTCAGCGATG AGGAAGATGG ACGAGACTCC 
GACGAGGAGG GAGCTGAGGG CCACAAGGAT TCCCAGTCAG CTGCTGCTGA GGAACCTGAG 
ACAGATGGCA AAAAGGGCAC CTCCCCTGGC AGCGAATTGC CCCCTCCCAA GACAGCCTGG 
ACAGAGAATG CTCGGCCTTC AGAGACAGAG CCAGCTCCTC CTACCCCAAA GCCTCCCCCA 
CCTCCACCTC ACCGGGGGCC AGTCGGGAAC TGGGGGCCCC CTGGGGACTA CCCAGATCGA 
GGGGGTCCTC CCTGCAAACC CCCAGCACCT GAGGATGAGG ATGAGGCATG GCGGCAGCGT 
CGGAAGCAAT CTTCTTCAGA GATCTCCCTG GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA 
GAGGAAGAGC GACGCATGCA GGAGGAGCGC CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT 
CTTGATGAAA AGTTTGGGGC ACCTGACAAG CGGCTCAAGG CAGAGCCTGC AGCCCCACCT 
GTTACCCCTG CTGCCCCAGC TCTACCACCT GTAGTCCCCA AAGAAATTCC TGCAGCTCCA 
GCTCTGCCAC CAACACCTAC CCCAACACCA GAGAAAGAAC CTGAAGAGCC GGCACAAGCC 
CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG GTGGCTCCAG TTCCCACTCT GGTGAGTGGG 
GGTGGTTGCA CTGCCAACAG CAACAGCAGT GGCAGCTTCG AAGCCAGCCC AGTGGAACCC 
CAACTGCCTT CAAAAGAGGG TCCTGAACCA CCAGAAGAGG TTCCTCCACC TACCACACCC 
CCAGCCCCAA AGATGGAACC CAAAGGTGAT GGGGTTGGTT CTACTCGCCA GCCCCCCAGT 
CAGGGCTTGG GCTACCCCAA ATACCAGAAG TCATTGCCTC CTCGCTTCCA GCGGCAGCAA 
CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG CAACAGCAGT GGCAGCAGCA GCAACAAGGC 
ACTGCCCCTC CTGCCCCAGT GCCCCCATCA CCACCACAAC CTGTGACCCT GGGAGCTGTA 
CCTGCCCCAC AAGCACCGCC CCCACCTCCC AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG 
CCCCCACCAA TGCCTCCAAT GAACTTTGAC CCCCGCTGGA TGATGATTCC TCCTTACGTG 
GACCCTCGGC TTCTCCAGGG CCGGCCCCCT CTGGACTTCT ACCCTCCTGG TGTGCATCCC 
TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC AGTGGGGGCT CAAGCTCAGA GCCATTTGAA 
CGCCATGCAC CCCCTCTGTT GCGGGAAAGG GGCACCCCAC CAGTGGACCC CAAGTTGGCC 
TGGGTTGGAG ATGTCTTTAC CACCACACCC ACTGATCCTC GTCCTCTTAC CTCACCACTG 
CGCCAGGCAG CAGATGAGGA GGAGAAGAGT ATGAGGAGCG AGACTCCTCC GGTACCCCCG 
CCCCCACCTT ATCTGGCTAA TTATCCAGGT TTTCCAGAGA ATGGAACTCC TGGGCCCCCA 
ATCTCGAGAT TCCCTCTGGA GGAATCTGCT CCCCCGGGAC CCCGCCCACT ACCCTGGCCT 
CCAGGCAATG ATGAAGCTGC CAAGATGCAG GCTCCACCGC CCAAGAAGGA ACCCTCGAAG 
GAGGAACCGC CACAGCTGTC CGGGCCAGAA GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA 
GGCCCACCAC CACCACGGAG AGAGAATCGT ACTGAGACTC GCTGGGGCCC CCGACCAGGC 
AGTTGTCGCC GTGGAATCCC TCCAGAGGAG CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT 
ATAAAGAAGC CACCACCACC TGTGAAAGTA GAGGAGCTGC CTCCTAAGTC CCTAGAACAG 
GGGGATGAGA CCCCCAAGGT CCCAAAGCCA GATGCACTCA AGACAGCTAA GGGCAAGGTG 
GGCCCCAAAG AAACCCCACC AGGTGGAAAC CTGTCTCCTG CACCTAGGCT TCGGCGGGAC 
TATTCCTATG AAAGAGTAGG TCCTACATCT TGCCGTGGTC GGGGCAGAGG CGAGTATTTT 
GCCAGAGGAA GGGGTTTTCG GGGGACCTAT GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT 
GAATTCCGAA GTTACCGAGA GTTCCGAGGA GACGATGGGC GTGGGGGTGG TTCAGGCGGA 
ACAAACCATC CTTCTGCTCC CCGAGGCCGC ACTGCCAGTG AGACACGAAG TGAGGGTTCA 
GAATATGAAG AAATCCCCAA GAGACGCCGC CAGCGTGGCT CTGAAACAGG CAGTGAGACT 
CATGAGAGTG ACCTGGCCCC CTCTGACAAG GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT 
GGTCAGGTCC CTCTTGCCCC CCCACAACCA GGAGCTCCTC CATCACCAGC CCCAGCCCGA 
TTCTCCACTG CCCGAGGTGG TCGTGTCTTC ACTCCAAGAG GAGTACCATC ACGCCGTGGC 
CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG 
GTTCCAAAGA AACCTCCAAC AGGTCCTTTG CCTCCAAGTA AAGAACCTTT GAAAGAGAAG 
CTGATCTCAG GGCCTCTGTC CCCCATGTCT CGAGCTGGCA ACATGGGTGT AGGCATGGAA 
GATGGGGAGC GACCCCGACG GAGGCGACAT GGCAGGGCTC AACAGCAGGA CAAACCACCT 
CGTTTCCGAA GGCTGAAACA AGAACGTGAA AACGCTGCCA GAGGAGCTGA TGGCAAGCCC 
CCTTCCCTAA CCCTTGCAGC CTCTACCCCT GGACCTGAGG AGACACTAAC AGCAGCCACA 
GTTCCCCCAC CCCCTCGCCG CACAGCTGCT AAGTCTCCTG ATCTGTCAAA CCAGAACTCA 
GACCAAGCCA ATGAGGAATG GGAGACTGCA TCAGAGAGCA GCGACTTTGC CAGTGAGCGC 
CGGGGTGACA AGGAGACTCC CCCAGCAGCA CTTATGACCT CTAAAGCTGT GGGGACTCCT 
GGGGCCAATG CAGGTGGGGC TGGACCAGGC ATTTCAGCCA TGTCTCGTGG AGACCTGAGC 
CAACGAGCCA AGGATTTGAG CAAGCGGAGC TTCTCAAGTC AGCGGCCAGG CATGGATCGT 
CAAAACCGGC GCCCAGGCAC TGGGGGCAAG ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT 
GGTGGTGCAG GCCCTGGGGG ACGGACCGGC CCAGGACGAG GGGATAAGAG GAGCTGGCCC 
TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG GAGCGACCTC CGGGACTTCC TCTGCCTCCG 
CCACCCCCCA GCAGTTCTGC TGTCTTCCGC CTGGACCAAG TTATCCATAG CAACCCTGCT 
GGAATCCAAC AGGCTCTGGC CCAACTTAGC AGCCGCCAAG GAAATGTAAC TGCACCAGGG 
GGGCACCCAA GGCCTAAGCC TGGGCCTCCC CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC 
CCAACCCGTT ATGACCCCCC AAGGGCCAGC AGCGCTATCA GTTCTGACCC CCACTTCGAG 
GAGCCAGGGC CGATGGTGAG GGGGGTGGGC GGGACTCCCC GGGACTCTGC CGGGGTTAAT 
CCCTTCCCCC CGAAACGGCG GGAGCGGCCT CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA 
ACTGTGCCAG CTTCTCATAG CTCTGGATTC TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT 
CAGGAAGAAT CCAGAGATTC AGGCACAGAG GCCCTAACTC CCCACATCTG GAATCGTTTA 
CATACTGCCA CAAGCCGAAA GAGTTACCAG CCTGGCTCCA TCGAGCCCTG GATGGAGCCC 
CTGAGTCCCT TTGAGGATGT GGCAGGCACA GAGATGAGTC AGTCTGACAG TGGAGTGGAC 
CTGAGTGGAG ATTCTCAGGT GTCATCAGGT CCCTGCAGTC AGCGAAGTTC CCCTGATGGA 
GGACTTAAGG GGTCAGCAGA GGGACCCCCC AAGCGGCCTG GAGGACCCTC ACCCCTGAAT 
GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT GAACCCTCTG AACCTCCTAG GAGACGGCCA 
CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT 
CCCATAGGCA CAGAACGATC ACAGCGCACA GATCGAGGCC CAGAGCCAGG CCCTCTCCGA 
CCAGCCCACC GACCGGGCTC TCAAGTTGAG TTTGGCACTA CCAACAAGGA CTCAGACTTG 
TGCCTAGTGG TCGGAGACAC TTTAAAAGGA GAAAAGGAGT TGGTGGCATC AGCCACAGAG 
GCCGTTCCCA TATCCAGAGA CTGGGAGCTG CTCCCTAGTG CTTCTACTTC AGCTGAGCCA 
CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT 
CCGTACCCTG AGGTCTTCTA TGGCAGCCCT GGACCTCCTA ATTCCCAGCA GGTCTCTGGG 
GGTGCACCCA TCGACTCTCA GTTACATCCC AACAGTGGAG GCTTCCGTCC TGGGACACCC 
TCATTGCACC AGTACAGGTC ACAGCCTCTG TATCTGCCCC CAGGTCCAGC CCCTCCCTCG 
GCACTGCTTT CTGGGGTAGC TCTCAAGGGC CAGTTTCTGG ATTTCTCAGC ACTGCAAGCC 
ACAGAGCTGG GGAAGTTGCC TGCTGGGGGA GTTCTCTACC CTCCGCCATC CTTCCTCTAC 
TCTGCAGCTT TCTGCCCCAG CCCTTTGCCT GACCCACCCT TGCTTCAGGT ACGCCAGGAT 
CTGCCATCCC CTTCAGATTT TTACTCTACT CCTCTGCAGC CTGGTGGCCA GAGTGGCTTC 
CTTCCTTCGG GAGCCCCTGC TCAGCAGATG CTTCTACCTG TGGTAGACTC ACAGCTGCCA 
GTGGTGAACT TTGGCTCCCT GCCACCAGCG CCACCCCCTG CTCCACCCCC CCTTTCTCTG 
TTACCTGTGG GTCCTGCTCT CCAGCCCCCC AACCTAGCTG TGCGGCCCCC ACCTGCTCCT 
GCTGCTCGGG TCTTACCTTC ACCCGCTAGA CCCTTCGCCC CTAGCTTGGG GCGAGCAGAG 
CTGCACCCAG TAGAACTAAA GCCTTTCCAG GATTACCGAA AGCTGAGCAG CAACCTTGGG 
GGTCCTGGGT CGTCACGGAC TCCCCCATCT GGAAGGCCCT TCTCTGGTCT CAATTCCCGT 
CTCAAGGCCC CACCTTCCAC CTACAGTGGA GTATTCCGAA CTCAGCGCAT TGACCTCTAC 
CAGCAGGCTT CCCCACCAGA TGCCCTGCGC TGGATGCCGA AGCCTTGGGA ACGGGCAGGG 
CCACCTTCTC GAGAAGGGCC TCCCCGAAGA GCAGAGGAGC CTGGGTCCCG GGGGGAGAAG 
GAGCCTGGAT TGCCCCCACC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prrc2a ( NM_020027.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064411.2 Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant 1, mRNA.

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CloneID	OMu15568
Clone ID Related Accession (Same CDS sequence)	XM_006524620.3 , XM_006524620.4 , NM_020027.3
Accession Version	NM_020027.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	protein PRRC2A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ107441.1 and BC053522.1. On Nov 19, 2010 this sequence version replaced NM_020027.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053522.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGGCAAGTCC TTAGAGATCC AGAAACCCGC TGTTGCCCCT 
CGACATGGCC TGCAGAGTCT CGGAAAAGTT GCCATTGCCC GGCGCATGCC ACCCCCAGCC 
AACCTTCCAA GCCTGAAAGC CGAGAACAAA GGCAATGACC CCAATGTCTC ACTAGTGCCG 
AAAGACGGAA CAGGATGGGC AAGCAAACAG GAGCAGTCCG ACCCCAAGAG TTCCGATGCC 
TCAACCGCTC AGCCGCCGGA ATCGCAGCCA CTGCCGGCTT CACAGACGCC TGCCTCCAAC 
CAGCCGAAAC GACCCCCAAC AGCCCCCGAG AACACTCCTT CGGTTCCAAG CGGGGTAAAG 
TCCTGGGCAC AAGCCAGTGT CACCCATGGA GCCCATGGAG ATGGTGGAAG GGCATCAAAC 
CTACTGTCGC GATTCTCTCG AGAGGAATTT CCGACCCTGC AGGCGGCTGG AGACCAGGAC 
AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA CAGTCGTCTG GGCCCGGACC AAGCCTCCGC 
CCCCAAAATT CTACAACTTG GAGGGACGGA GGTGGGCGTG GCCCTGATGA CCTGGAGGGC 
CCGGACTCCA AACTTCATCA TGGCCATGAT CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA 
CCCCAGTTCC CTCCCTACCG CGGGATGATG CCGCCCTTTA TGTATCCCCC ATATCTCCCA 
TTCCCTCCGC CCTATGGACC CCAGGGGCCT TATAGATACC CGACTCCTGA TGGGCCCAGC 
CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC TCAGGGCCAC CAATGCGCCT TGTAGAGCCT 
GTGGGTCGGC CTTCTATTCT TAAAGAGGAT AATCTTAAAG AGTTTGACCA ACTGGACCAG 
GAAAATGATG ATGGTTGGGC AGGGGCCCAT GAAGAGGTTG ACTATACTGA AAAGCTCAAA 
TTCAGCGATG AGGAAGATGG ACGAGACTCC GACGAGGAGG GAGCTGAGGG CCACAAGGAT 
TCCCAGTCAG CTGCTGCTGA GGAACCTGAG ACAGATGGCA AAAAGGGCAC CTCCCCTGGC 
AGCGAATTGC CCCCTCCCAA GACAGCCTGG ACAGAGAATG CTCGGCCTTC AGAGACAGAG 
CCAGCTCCTC CTACCCCAAA GCCTCCCCCA CCTCCACCTC ACCGGGGGCC AGTCGGGAAC 
TGGGGGCCCC CTGGGGACTA CCCAGATCGA GGGGGTCCTC CCTGCAAACC CCCAGCACCT 
GAGGATGAGG ATGAGGCATG GCGGCAGCGT CGGAAGCAAT CTTCTTCAGA GATCTCCCTG 
GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA GAGGAAGAGC GACGCATGCA GGAGGAGCGC 
CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT CTTGATGAAA AGTTTGGGGC ACCTGACAAG 
CGGCTCAAGG CAGAGCCTGC AGCCCCACCT GTTACCCCTG CTGCCCCAGC TCTACCACCT 
GTAGTCCCCA AAGAAATTCC TGCAGCTCCA GCTCTGCCAC CAACACCTAC CCCAACACCA 
GAGAAAGAAC CTGAAGAGCC GGCACAAGCC CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG 
GTGGCTCCAG TTCCCACTCT GGTGAGTGGG GGTGGTTGCA CTGCCAACAG CAACAGCAGT 
GGCAGCTTCG AAGCCAGCCC AGTGGAACCC CAACTGCCTT CAAAAGAGGG TCCTGAACCA 
CCAGAAGAGG TTCCTCCACC TACCACACCC CCAGCCCCAA AGATGGAACC CAAAGGTGAT 
GGGGTTGGTT CTACTCGCCA GCCCCCCAGT CAGGGCTTGG GCTACCCCAA ATACCAGAAG 
TCATTGCCTC CTCGCTTCCA GCGGCAGCAA CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG 
CAACAGCAGT GGCAGCAGCA GCAACAAGGC ACTGCCCCTC CTGCCCCAGT GCCCCCATCA 
CCACCACAAC CTGTGACCCT GGGAGCTGTA CCTGCCCCAC AAGCACCGCC CCCACCTCCC 
AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG CCCCCACCAA TGCCTCCAAT GAACTTTGAC 
CCCCGCTGGA TGATGATTCC TCCTTACGTG GACCCTCGGC TTCTCCAGGG CCGGCCCCCT 
CTGGACTTCT ACCCTCCTGG TGTGCATCCC TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC 
AGTGGGGGCT CAAGCTCAGA GCCATTTGAA CGCCATGCAC CCCCTCTGTT GCGGGAAAGG 
GGCACCCCAC CAGTGGACCC CAAGTTGGCC TGGGTTGGAG ATGTCTTTAC CACCACACCC 
ACTGATCCTC GTCCTCTTAC CTCACCACTG CGCCAGGCAG CAGATGAGGA GGAGAAGAGT 
ATGAGGAGCG AGACTCCTCC GGTACCCCCG CCCCCACCTT ATCTGGCTAA TTATCCAGGT 
TTTCCAGAGA ATGGAACTCC TGGGCCCCCA ATCTCGAGAT TCCCTCTGGA GGAATCTGCT 
CCCCCGGGAC CCCGCCCACT ACCCTGGCCT CCAGGCAATG ATGAAGCTGC CAAGATGCAG 
GCTCCACCGC CCAAGAAGGA ACCCTCGAAG GAGGAACCGC CACAGCTGTC CGGGCCAGAA 
GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA GGCCCACCAC CACCACGGAG AGAGAATCGT 
ACTGAGACTC GCTGGGGCCC CCGACCAGGC AGTTGTCGCC GTGGAATCCC TCCAGAGGAG 
CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT ATAAAGAAGC CACCACCACC TGTGAAAGTA 
GAGGAGCTGC CTCCTAAGTC CCTAGAACAG GGGGATGAGA CCCCCAAGGT CCCAAAGCCA 
GATGCACTCA AGACAGCTAA GGGCAAGGTG GGCCCCAAAG AAACCCCACC AGGTGGAAAC 
CTGTCTCCTG CACCTAGGCT TCGGCGGGAC TATTCCTATG AAAGAGTAGG TCCTACATCT 
TGCCGTGGTC GGGGCAGAGG CGAGTATTTT GCCAGAGGAA GGGGTTTTCG GGGGACCTAT 
GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT GAATTCCGAA GTTACCGAGA GTTCCGAGGA 
GACGATGGGC GTGGGGGTGG TTCAGGCGGA ACAAACCATC CTTCTGCTCC CCGAGGCCGC 
ACTGCCAGTG AGACACGAAG TGAGGGTTCA GAATATGAAG AAATCCCCAA GAGACGCCGC 
CAGCGTGGCT CTGAAACAGG CAGTGAGACT CATGAGAGTG ACCTGGCCCC CTCTGACAAG 
GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT GGTCAGGTCC CTCTTGCCCC CCCACAACCA 
GGAGCTCCTC CATCACCAGC CCCAGCCCGA TTCTCCACTG CCCGAGGTGG TCGTGTCTTC 
ACTCCAAGAG GAGTACCATC ACGCCGTGGC CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC 
TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG GTTCCAAAGA AACCTCCAAC AGGTCCTTTG 
CCTCCAAGTA AAGAACCTTT GAAAGAGAAG CTGATCTCAG GGCCTCTGTC CCCCATGTCT 
CGAGCTGGCA ACATGGGTGT AGGCATGGAA GATGGGGAGC GACCCCGACG GAGGCGACAT 
GGCAGGGCTC AACAGCAGGA CAAACCACCT CGTTTCCGAA GGCTGAAACA AGAACGTGAA 
AACGCTGCCA GAGGAGCTGA TGGCAAGCCC CCTTCCCTAA CCCTTGCAGC CTCTACCCCT 
GGACCTGAGG AGACACTAAC AGCAGCCACA GTTCCCCCAC CCCCTCGCCG CACAGCTGCT 
AAGTCTCCTG ATCTGTCAAA CCAGAACTCA GACCAAGCCA ATGAGGAATG GGAGACTGCA 
TCAGAGAGCA GCGACTTTGC CAGTGAGCGC CGGGGTGACA AGGAGACTCC CCCAGCAGCA 
CTTATGACCT CTAAAGCTGT GGGGACTCCT GGGGCCAATG CAGGTGGGGC TGGACCAGGC 
ATTTCAGCCA TGTCTCGTGG AGACCTGAGC CAACGAGCCA AGGATTTGAG CAAGCGGAGC 
TTCTCAAGTC AGCGGCCAGG CATGGATCGT CAAAACCGGC GCCCAGGCAC TGGGGGCAAG 
ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT GGTGGTGCAG GCCCTGGGGG ACGGACCGGC 
CCAGGACGAG GGGATAAGAG GAGCTGGCCC TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG 
GAGCGACCTC CGGGACTTCC TCTGCCTCCG CCACCCCCCA GCAGTTCTGC TGTCTTCCGC 
CTGGACCAAG TTATCCATAG CAACCCTGCT GGAATCCAAC AGGCTCTGGC CCAACTTAGC 
AGCCGCCAAG GAAATGTAAC TGCACCAGGG GGGCACCCAA GGCCTAAGCC TGGGCCTCCC 
CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC CCAACCCGTT ATGACCCCCC AAGGGCCAGC 
AGCGCTATCA GTTCTGACCC CCACTTCGAG GAGCCAGGGC CGATGGTGAG GGGGGTGGGC 
GGGACTCCCC GGGACTCTGC CGGGGTTAAT CCCTTCCCCC CGAAACGGCG GGAGCGGCCT 
CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA ACTGTGCCAG CTTCTCATAG CTCTGGATTC 
TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT CAGGAAGAAT CCAGAGATTC AGGCACAGAG 
GCCCTAACTC CCCACATCTG GAATCGTTTA CATACTGCCA CAAGCCGAAA GAGTTACCAG 
CCTGGCTCCA TCGAGCCCTG GATGGAGCCC CTGAGTCCCT TTGAGGATGT GGCAGGCACA 
GAGATGAGTC AGTCTGACAG TGGAGTGGAC CTGAGTGGAG ATTCTCAGGT GTCATCAGGT 
CCCTGCAGTC AGCGAAGTTC CCCTGATGGA GGACTTAAGG GGTCAGCAGA GGGACCCCCC 
AAGCGGCCTG GAGGACCCTC ACCCCTGAAT GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT 
GAACCCTCTG AACCTCCTAG GAGACGGCCA CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG 
CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT CCCATAGGCA CAGAACGATC ACAGCGCACA 
GATCGAGGCC CAGAGCCAGG CCCTCTCCGA CCAGCCCACC GACCGGGCTC TCAAGTTGAG 
TTTGGCACTA CCAACAAGGA CTCAGACTTG TGCCTAGTGG TCGGAGACAC TTTAAAAGGA 
GAAAAGGAGT TGGTGGCATC AGCCACAGAG GCCGTTCCCA TATCCAGAGA CTGGGAGCTG 
CTCCCTAGTG CTTCTACTTC AGCTGAGCCA CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT 
GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT CCGTACCCTG AGGTCTTCTA TGGCAGCCCT 
GGACCTCCTA ATTCCCAGCA GGTCTCTGGG GGTGCACCCA TCGACTCTCA GTTACATCCC 
AACAGTGGAG GCTTCCGTCC TGGGACACCC TCATTGCACC AGTACAGGTC ACAGCCTCTG 
TATCTGCCCC CAGGTCCAGC CCCTCCCTCG GCACTGCTTT CTGGGGTAGC TCTCAAGGGC 
CAGTTTCTGG ATTTCTCAGC ACTGCAAGCC ACAGAGCTGG GGAAGTTGCC TGCTGGGGGA 
GTTCTCTACC CTCCGCCATC CTTCCTCTAC TCTGCAGCTT TCTGCCCCAG CCCTTTGCCT 
GACCCACCCT TGCTTCAGGT ACGCCAGGAT CTGCCATCCC CTTCAGATTT TTACTCTACT 
CCTCTGCAGC CTGGTGGCCA GAGTGGCTTC CTTCCTTCGG GAGCCCCTGC TCAGCAGATG 
CTTCTACCTG TGGTAGACTC ACAGCTGCCA GTGGTGAACT TTGGCTCCCT GCCACCAGCG 
CCACCCCCTG CTCCACCCCC CCTTTCTCTG TTACCTGTGG GTCCTGCTCT CCAGCCCCCC 
AACCTAGCTG TGCGGCCCCC ACCTGCTCCT GCTGCTCGGG TCTTACCTTC ACCCGCTAGA 
CCCTTCGCCC CTAGCTTGGG GCGAGCAGAG CTGCACCCAG TAGAACTAAA GCCTTTCCAG 
GATTACCGAA AGCTGAGCAG CAACCTTGGG GGTCCTGGGT CGTCACGGAC TCCCCCATCT 
GGAAGGCCCT TCTCTGGTCT CAATTCCCGT CTCAAGGCCC CACCTTCCAC CTACAGTGGA 
GTATTCCGAA CTCAGCGCAT TGACCTCTAC CAGCAGGCTT CCCCACCAGA TGCCCTGCGC 
TGGATGCCGA AGCCTTGGGA ACGGGCAGGG CCACCTTCTC GAGAAGGGCC TCCCCGAAGA 
GCAGAGGAGC CTGGGTCCCG GGGGGAGAAG GAGCCTGGAT TGCCCCCACC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064411.2
CDS	207..6683
Translation	MSDRSGPTAKGKDGKKYSSLNLFDTYKGKSLEIQKPAVAPRHGLQSLGKVAIARRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQSDPKSSDASTAQPPESQPLPASQTPASNQPKRPPTAPENTPSVPSGVKSWAQASVTHGAHGDGGRASNLLSRFSREEFPTLQAAGDQDKAAKERESAEQSSGPGPSLRPQNSTTWRDGGGRGPDDLEGPDSKLHHGHDPRGGLQPSGPPQFPPYRGMMPPFMYPPYLPFPPPYGPQGPYRYPTPDGPSRFPRVAGPRGSGPPMRLVEPVGRPSILKEDNLKEFDQLDQENDDGWAGAHEEVDYTEKLKFSDEEDGRDSDEEGAEGHKDSQSAAAEEPETDGKKGTSPGSELPPPKTAWTENARPSETEPAPPTPKPPPPPPHRGPVGNWGPPGDYPDRGGPPCKPPAPEDEDEAWRQRRKQSSSEISLAVERARRRREEEERRMQEERRAACAEKLKRLDEKFGAPDKRLKAEPAAPPVTPAAPALPPVVPKEIPAAPALPPTPTPTPEKEPEEPAQAPPVQAAPSPGVAPVPTLVSGGGCTANSNSSGSFEASPVEPQLPSKEGPEPPEEVPPPTTPPAPKMEPKGDGVGSTRQPPSQGLGYPKYQKSLPPRFQRQQQEQLLKQQQQQQQWQQQQQGTAPPAPVPPSPPQPVTLGAVPAPQAPPPPPKALYPGALGRPPPMPPMNFDPRWMMIPPYVDPRLLQGRPPLDFYPPGVHPSGLVPRERSDSGGSSSEPFERHAPPLLRERGTPPVDPKLAWVGDVFTTTPTDPRPLTSPLRQAADEEEKSMRSETPPVPPPPPYLANYPGFPENGTPGPPISRFPLEESAPPGPRPLPWPPGNDEAAKMQAPPPKKEPSKEEPPQLSGPEAGRKPARGGQGPPPPRRENRTETRWGPRPGSCRRGIPPEEPGVPPRRAGPIKKPPPPVKVEELPPKSLEQGDETPKVPKPDALKTAKGKVGPKETPPGGNLSPAPRLRRDYSYERVGPTSCRGRGRGEYFARGRGFRGTYGGRGRGARSREFRSYREFRGDDGRGGGSGGTNHPSAPRGRTASETRSEGSEYEEIPKRRRQRGSETGSETHESDLAPSDKEAPPPKEGVLGQVPLAPPQPGAPPSPAPARFSTARGGRVFTPRGVPSRRGRGGGRPPPVCSGWSPPAKSLVPKKPPTGPLPPSKEPLKEKLISGPLSPMSRAGNMGVGMEDGERPRRRRHGRAQQQDKPPRFRRLKQERENAARGADGKPPSLTLAASTPGPEETLTAATVPPPPRRTAAKSPDLSNQNSDQANEEWETASESSDFASERRGDKETPPAALMTSKAVGTPGANAGGAGPGISAMSRGDLSQRAKDLSKRSFSSQRPGMDRQNRRPGTGGKTGSGGGSSGGGGAGPGGRTGPGRGDKRSWPSPKNRSRPPEERPPGLPLPPPPPSSSAVFRLDQVIHSNPAGIQQALAQLSSRQGNVTAPGGHPRPKPGPPQAPQGSSPRPPTRYDPPRASSAISSDPHFEEPGPMVRGVGGTPRDSAGVNPFPPKRRERPPRKPELLQEETVPASHSSGFLGSKPEVPGPQEESRDSGTEALTPHIWNRLHTATSRKSYQPGSIEPWMEPLSPFEDVAGTEMSQSDSGVDLSGDSQVSSGPCSQRSSPDGGLKGSAEGPPKRPGGPSPLNAVPGESASGSEPSEPPRRRPPASHEGERKELPREQPLPPGPIGTERSQRTDRGPEPGPLRPAHRPGSQVEFGTTNKDSDLCLVVGDTLKGEKELVASATEAVPISRDWELLPSASTSAEPQPKSLGSGQCVPEPSPSGQRPYPEVFYGSPGPPNSQQVSGGAPIDSQLHPNSGGFRPGTPSLHQYRSQPLYLPPGPAPPSALLSGVALKGQFLDFSALQATELGKLPAGGVLYPPPSFLYSAAFCPSPLPDPPLLQVRQDLPSPSDFYSTPLQPGGQSGFLPSGAPAQQMLLPVVDSQLPVVNFGSLPPAPPPAPPPLSLLPVGPALQPPNLAVRPPPAPAARVLPSPARPFAPSLGRAELHPVELKPFQDYRKLSSNLGGPGSSRTPPSGRPFSGLNSRLKAPPSTYSGVFRTQRIDLYQQASPPDALRWMPKPWERAGPPSREGPPRRAEEPGSRGEKEPGLPPPR

Target ORF information:

RefSeq Version	NM_020027.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020027.3

ORF Insert Sequence:

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGGCAAGTCC TTAGAGATCC AGAAACCCGC TGTTGCCCCT 
CGACATGGCC TGCAGAGTCT CGGAAAAGTT GCCATTGCCC GGCGCATGCC ACCCCCAGCC 
AACCTTCCAA GCCTGAAAGC CGAGAACAAA GGCAATGACC CCAATGTCTC ACTAGTGCCG 
AAAGACGGAA CAGGATGGGC AAGCAAACAG GAGCAGTCCG ACCCCAAGAG TTCCGATGCC 
TCAACCGCTC AGCCGCCGGA ATCGCAGCCA CTGCCGGCTT CACAGACGCC TGCCTCCAAC 
CAGCCGAAAC GACCCCCAAC AGCCCCCGAG AACACTCCTT CGGTTCCAAG CGGGGTAAAG 
TCCTGGGCAC AAGCCAGTGT CACCCATGGA GCCCATGGAG ATGGTGGAAG GGCATCAAAC 
CTACTGTCGC GATTCTCTCG AGAGGAATTT CCGACCCTGC AGGCGGCTGG AGACCAGGAC 
AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA CAGTCGTCTG GGCCCGGACC AAGCCTCCGC 
CCCCAAAATT CTACAACTTG GAGGGACGGA GGTGGGCGTG GCCCTGATGA CCTGGAGGGC 
CCGGACTCCA AACTTCATCA TGGCCATGAT CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA 
CCCCAGTTCC CTCCCTACCG CGGGATGATG CCGCCCTTTA TGTATCCCCC ATATCTCCCA 
TTCCCTCCGC CCTATGGACC CCAGGGGCCT TATAGATACC CGACTCCTGA TGGGCCCAGC 
CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC TCAGGGCCAC CAATGCGCCT TGTAGAGCCT 
GTGGGTCGGC CTTCTATTCT TAAAGAGGAT AATCTTAAAG AGTTTGACCA ACTGGACCAG 
GAAAATGATG ATGGTTGGGC AGGGGCCCAT GAAGAGGTTG ACTATACTGA AAAGCTCAAA 
TTCAGCGATG AGGAAGATGG ACGAGACTCC GACGAGGAGG GAGCTGAGGG CCACAAGGAT 
TCCCAGTCAG CTGCTGCTGA GGAACCTGAG ACAGATGGCA AAAAGGGCAC CTCCCCTGGC 
AGCGAATTGC CCCCTCCCAA GACAGCCTGG ACAGAGAATG CTCGGCCTTC AGAGACAGAG 
CCAGCTCCTC CTACCCCAAA GCCTCCCCCA CCTCCACCTC ACCGGGGGCC AGTCGGGAAC 
TGGGGGCCCC CTGGGGACTA CCCAGATCGA GGGGGTCCTC CCTGCAAACC CCCAGCACCT 
GAGGATGAGG ATGAGGCATG GCGGCAGCGT CGGAAGCAAT CTTCTTCAGA GATCTCCCTG 
GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA GAGGAAGAGC GACGCATGCA GGAGGAGCGC 
CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT CTTGATGAAA AGTTTGGGGC ACCTGACAAG 
CGGCTCAAGG CAGAGCCTGC AGCCCCACCT GTTACCCCTG CTGCCCCAGC TCTACCACCT 
GTAGTCCCCA AAGAAATTCC TGCAGCTCCA GCTCTGCCAC CAACACCTAC CCCAACACCA 
GAGAAAGAAC CTGAAGAGCC GGCACAAGCC CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG 
GTGGCTCCAG TTCCCACTCT GGTGAGTGGG GGTGGTTGCA CTGCCAACAG CAACAGCAGT 
GGCAGCTTCG AAGCCAGCCC AGTGGAACCC CAACTGCCTT CAAAAGAGGG TCCTGAACCA 
CCAGAAGAGG TTCCTCCACC TACCACACCC CCAGCCCCAA AGATGGAACC CAAAGGTGAT 
GGGGTTGGTT CTACTCGCCA GCCCCCCAGT CAGGGCTTGG GCTACCCCAA ATACCAGAAG 
TCATTGCCTC CTCGCTTCCA GCGGCAGCAA CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG 
CAACAGCAGT GGCAGCAGCA GCAACAAGGC ACTGCCCCTC CTGCCCCAGT GCCCCCATCA 
CCACCACAAC CTGTGACCCT GGGAGCTGTA CCTGCCCCAC AAGCACCGCC CCCACCTCCC 
AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG CCCCCACCAA TGCCTCCAAT GAACTTTGAC 
CCCCGCTGGA TGATGATTCC TCCTTACGTG GACCCTCGGC TTCTCCAGGG CCGGCCCCCT 
CTGGACTTCT ACCCTCCTGG TGTGCATCCC TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC 
AGTGGGGGCT CAAGCTCAGA GCCATTTGAA CGCCATGCAC CCCCTCTGTT GCGGGAAAGG 
GGCACCCCAC CAGTGGACCC CAAGTTGGCC TGGGTTGGAG ATGTCTTTAC CACCACACCC 
ACTGATCCTC GTCCTCTTAC CTCACCACTG CGCCAGGCAG CAGATGAGGA GGAGAAGAGT 
ATGAGGAGCG AGACTCCTCC GGTACCCCCG CCCCCACCTT ATCTGGCTAA TTATCCAGGT 
TTTCCAGAGA ATGGAACTCC TGGGCCCCCA ATCTCGAGAT TCCCTCTGGA GGAATCTGCT 
CCCCCGGGAC CCCGCCCACT ACCCTGGCCT CCAGGCAATG ATGAAGCTGC CAAGATGCAG 
GCTCCACCGC CCAAGAAGGA ACCCTCGAAG GAGGAACCGC CACAGCTGTC CGGGCCAGAA 
GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA GGCCCACCAC CACCACGGAG AGAGAATCGT 
ACTGAGACTC GCTGGGGCCC CCGACCAGGC AGTTGTCGCC GTGGAATCCC TCCAGAGGAG 
CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT ATAAAGAAGC CACCACCACC TGTGAAAGTA 
GAGGAGCTGC CTCCTAAGTC CCTAGAACAG GGGGATGAGA CCCCCAAGGT CCCAAAGCCA 
GATGCACTCA AGACAGCTAA GGGCAAGGTG GGCCCCAAAG AAACCCCACC AGGTGGAAAC 
CTGTCTCCTG CACCTAGGCT TCGGCGGGAC TATTCCTATG AAAGAGTAGG TCCTACATCT 
TGCCGTGGTC GGGGCAGAGG CGAGTATTTT GCCAGAGGAA GGGGTTTTCG GGGGACCTAT 
GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT GAATTCCGAA GTTACCGAGA GTTCCGAGGA 
GACGATGGGC GTGGGGGTGG TTCAGGCGGA ACAAACCATC CTTCTGCTCC CCGAGGCCGC 
ACTGCCAGTG AGACACGAAG TGAGGGTTCA GAATATGAAG AAATCCCCAA GAGACGCCGC 
CAGCGTGGCT CTGAAACAGG CAGTGAGACT CATGAGAGTG ACCTGGCCCC CTCTGACAAG 
GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT GGTCAGGTCC CTCTTGCCCC CCCACAACCA 
GGAGCTCCTC CATCACCAGC CCCAGCCCGA TTCTCCACTG CCCGAGGTGG TCGTGTCTTC 
ACTCCAAGAG GAGTACCATC ACGCCGTGGC CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC 
TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG GTTCCAAAGA AACCTCCAAC AGGTCCTTTG 
CCTCCAAGTA AAGAACCTTT GAAAGAGAAG CTGATCTCAG GGCCTCTGTC CCCCATGTCT 
CGAGCTGGCA ACATGGGTGT AGGCATGGAA GATGGGGAGC GACCCCGACG GAGGCGACAT 
GGCAGGGCTC AACAGCAGGA CAAACCACCT CGTTTCCGAA GGCTGAAACA AGAACGTGAA 
AACGCTGCCA GAGGAGCTGA TGGCAAGCCC CCTTCCCTAA CCCTTGCAGC CTCTACCCCT 
GGACCTGAGG AGACACTAAC AGCAGCCACA GTTCCCCCAC CCCCTCGCCG CACAGCTGCT 
AAGTCTCCTG ATCTGTCAAA CCAGAACTCA GACCAAGCCA ATGAGGAATG GGAGACTGCA 
TCAGAGAGCA GCGACTTTGC CAGTGAGCGC CGGGGTGACA AGGAGACTCC CCCAGCAGCA 
CTTATGACCT CTAAAGCTGT GGGGACTCCT GGGGCCAATG CAGGTGGGGC TGGACCAGGC 
ATTTCAGCCA TGTCTCGTGG AGACCTGAGC CAACGAGCCA AGGATTTGAG CAAGCGGAGC 
TTCTCAAGTC AGCGGCCAGG CATGGATCGT CAAAACCGGC GCCCAGGCAC TGGGGGCAAG 
ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT GGTGGTGCAG GCCCTGGGGG ACGGACCGGC 
CCAGGACGAG GGGATAAGAG GAGCTGGCCC TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG 
GAGCGACCTC CGGGACTTCC TCTGCCTCCG CCACCCCCCA GCAGTTCTGC TGTCTTCCGC 
CTGGACCAAG TTATCCATAG CAACCCTGCT GGAATCCAAC AGGCTCTGGC CCAACTTAGC 
AGCCGCCAAG GAAATGTAAC TGCACCAGGG GGGCACCCAA GGCCTAAGCC TGGGCCTCCC 
CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC CCAACCCGTT ATGACCCCCC AAGGGCCAGC 
AGCGCTATCA GTTCTGACCC CCACTTCGAG GAGCCAGGGC CGATGGTGAG GGGGGTGGGC 
GGGACTCCCC GGGACTCTGC CGGGGTTAAT CCCTTCCCCC CGAAACGGCG GGAGCGGCCT 
CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA ACTGTGCCAG CTTCTCATAG CTCTGGATTC 
TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT CAGGAAGAAT CCAGAGATTC AGGCACAGAG 
GCCCTAACTC CCCACATCTG GAATCGTTTA CATACTGCCA CAAGCCGAAA GAGTTACCAG 
CCTGGCTCCA TCGAGCCCTG GATGGAGCCC CTGAGTCCCT TTGAGGATGT GGCAGGCACA 
GAGATGAGTC AGTCTGACAG TGGAGTGGAC CTGAGTGGAG ATTCTCAGGT GTCATCAGGT 
CCCTGCAGTC AGCGAAGTTC CCCTGATGGA GGACTTAAGG GGTCAGCAGA GGGACCCCCC 
AAGCGGCCTG GAGGACCCTC ACCCCTGAAT GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT 
GAACCCTCTG AACCTCCTAG GAGACGGCCA CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG 
CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT CCCATAGGCA CAGAACGATC ACAGCGCACA 
GATCGAGGCC CAGAGCCAGG CCCTCTCCGA CCAGCCCACC GACCGGGCTC TCAAGTTGAG 
TTTGGCACTA CCAACAAGGA CTCAGACTTG TGCCTAGTGG TCGGAGACAC TTTAAAAGGA 
GAAAAGGAGT TGGTGGCATC AGCCACAGAG GCCGTTCCCA TATCCAGAGA CTGGGAGCTG 
CTCCCTAGTG CTTCTACTTC AGCTGAGCCA CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT 
GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT CCGTACCCTG AGGTCTTCTA TGGCAGCCCT 
GGACCTCCTA ATTCCCAGCA GGTCTCTGGG GGTGCACCCA TCGACTCTCA GTTACATCCC 
AACAGTGGAG GCTTCCGTCC TGGGACACCC TCATTGCACC AGTACAGGTC ACAGCCTCTG 
TATCTGCCCC CAGGTCCAGC CCCTCCCTCG GCACTGCTTT CTGGGGTAGC TCTCAAGGGC 
CAGTTTCTGG ATTTCTCAGC ACTGCAAGCC ACAGAGCTGG GGAAGTTGCC TGCTGGGGGA 
GTTCTCTACC CTCCGCCATC CTTCCTCTAC TCTGCAGCTT TCTGCCCCAG CCCTTTGCCT 
GACCCACCCT TGCTTCAGGT ACGCCAGGAT CTGCCATCCC CTTCAGATTT TTACTCTACT 
CCTCTGCAGC CTGGTGGCCA GAGTGGCTTC CTTCCTTCGG GAGCCCCTGC TCAGCAGATG 
CTTCTACCTG TGGTAGACTC ACAGCTGCCA GTGGTGAACT TTGGCTCCCT GCCACCAGCG 
CCACCCCCTG CTCCACCCCC CCTTTCTCTG TTACCTGTGG GTCCTGCTCT CCAGCCCCCC 
AACCTAGCTG TGCGGCCCCC ACCTGCTCCT GCTGCTCGGG TCTTACCTTC ACCCGCTAGA 
CCCTTCGCCC CTAGCTTGGG GCGAGCAGAG CTGCACCCAG TAGAACTAAA GCCTTTCCAG 
GATTACCGAA AGCTGAGCAG CAACCTTGGG GGTCCTGGGT CGTCACGGAC TCCCCCATCT 
GGAAGGCCCT TCTCTGGTCT CAATTCCCGT CTCAAGGCCC CACCTTCCAC CTACAGTGGA 
GTATTCCGAA CTCAGCGCAT TGACCTCTAC CAGCAGGCTT CCCCACCAGA TGCCCTGCGC 
TGGATGCCGA AGCCTTGGGA ACGGGCAGGG CCACCTTCTC GAGAAGGGCC TCCCCGAAGA 
GCAGAGGAGC CTGGGTCCCG GGGGGAGAAG GAGCCTGGAT TGCCCCCACC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prrc2a ( NM_001199044.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001185973.1 Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant 2, mRNA.

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CloneID	OMu15766
Clone ID Related Accession (Same CDS sequence)	NM_001199044.1 , XM_030249899.1
Accession Version	NM_001199044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	protein PRRC2A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ107441.1, BC053522.1 and BC052630.1. Transcript Variant: This variant (2) uses an alternate splice acceptor site in the 3' coding region, compared to variant 1, which results in a protein (isoform 2) that is one amino acid shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052630.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGGCAAGTCC TTAGAGATCC AGAAACCCGC TGTTGCCCCT 
CGACATGGCC TGCAGAGTCT CGGAAAAGTT GCCATTGCCC GGCGCATGCC ACCCCCAGCC 
AACCTTCCAA GCCTGAAAGC CGAGAACAAA GGCAATGACC CCAATGTCTC ACTAGTGCCG 
AAAGACGGAA CAGGATGGGC AAGCAAACAG GAGCAGTCCG ACCCCAAGAG TTCCGATGCC 
TCAACCGCTC AGCCGCCGGA ATCGCAGCCA CTGCCGGCTT CACAGACGCC TGCCTCCAAC 
CAGCCGAAAC GACCCCCAAC AGCCCCCGAG AACACTCCTT CGGTTCCAAG CGGGGTAAAG 
TCCTGGGCAC AAGCCAGTGT CACCCATGGA GCCCATGGAG ATGGTGGAAG GGCATCAAAC 
CTACTGTCGC GATTCTCTCG AGAGGAATTT CCGACCCTGC AGGCGGCTGG AGACCAGGAC 
AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA CAGTCGTCTG GGCCCGGACC AAGCCTCCGC 
CCCCAAAATT CTACAACTTG GAGGGACGGA GGTGGGCGTG GCCCTGATGA CCTGGAGGGC 
CCGGACTCCA AACTTCATCA TGGCCATGAT CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA 
CCCCAGTTCC CTCCCTACCG CGGGATGATG CCGCCCTTTA TGTATCCCCC ATATCTCCCA 
TTCCCTCCGC CCTATGGACC CCAGGGGCCT TATAGATACC CGACTCCTGA TGGGCCCAGC 
CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC TCAGGGCCAC CAATGCGCCT TGTAGAGCCT 
GTGGGTCGGC CTTCTATTCT TAAAGAGGAT AATCTTAAAG AGTTTGACCA ACTGGACCAG 
GAAAATGATG ATGGTTGGGC AGGGGCCCAT GAAGAGGTTG ACTATACTGA AAAGCTCAAA 
TTCAGCGATG AGGAAGATGG ACGAGACTCC GACGAGGAGG GAGCTGAGGG CCACAAGGAT 
TCCCAGTCAG CTGCTGCTGA GGAACCTGAG ACAGATGGCA AAAAGGGCAC CTCCCCTGGC 
AGCGAATTGC CCCCTCCCAA GACAGCCTGG ACAGAGAATG CTCGGCCTTC AGAGACAGAG 
CCAGCTCCTC CTACCCCAAA GCCTCCCCCA CCTCCACCTC ACCGGGGGCC AGTCGGGAAC 
TGGGGGCCCC CTGGGGACTA CCCAGATCGA GGGGGTCCTC CCTGCAAACC CCCAGCACCT 
GAGGATGAGG ATGAGGCATG GCGGCAGCGT CGGAAGCAAT CTTCTTCAGA GATCTCCCTG 
GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA GAGGAAGAGC GACGCATGCA GGAGGAGCGC 
CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT CTTGATGAAA AGTTTGGGGC ACCTGACAAG 
CGGCTCAAGG CAGAGCCTGC AGCCCCACCT GTTACCCCTG CTGCCCCAGC TCTACCACCT 
GTAGTCCCCA AAGAAATTCC TGCAGCTCCA GCTCTGCCAC CAACACCTAC CCCAACACCA 
GAGAAAGAAC CTGAAGAGCC GGCACAAGCC CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG 
GTGGCTCCAG TTCCCACTCT GGTGAGTGGG GGTGGTTGCA CTGCCAACAG CAACAGCAGT 
GGCAGCTTCG AAGCCAGCCC AGTGGAACCC CAACTGCCTT CAAAAGAGGG TCCTGAACCA 
CCAGAAGAGG TTCCTCCACC TACCACACCC CCAGCCCCAA AGATGGAACC CAAAGGTGAT 
GGGGTTGGTT CTACTCGCCA GCCCCCCAGT CAGGGCTTGG GCTACCCCAA ATACCAGAAG 
TCATTGCCTC CTCGCTTCCA GCGGCAGCAA CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG 
CAACAGCAGT GGCAGCAGCA GCAACAAGGC ACTGCCCCTC CTGCCCCAGT GCCCCCATCA 
CCACCACAAC CTGTGACCCT GGGAGCTGTA CCTGCCCCAC AAGCACCGCC CCCACCTCCC 
AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG CCCCCACCAA TGCCTCCAAT GAACTTTGAC 
CCCCGCTGGA TGATGATTCC TCCTTACGTG GACCCTCGGC TTCTCCAGGG CCGGCCCCCT 
CTGGACTTCT ACCCTCCTGG TGTGCATCCC TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC 
AGTGGGGGCT CAAGCTCAGA GCCATTTGAA CGCCATGCAC CCCCTCTGTT GCGGGAAAGG 
GGCACCCCAC CAGTGGACCC CAAGTTGGCC TGGGTTGGAG ATGTCTTTAC CACCACACCC 
ACTGATCCTC GTCCTCTTAC CTCACCACTG CGCCAGGCAG CAGATGAGGA GGAGAAGAGT 
ATGAGGAGCG AGACTCCTCC GGTACCCCCG CCCCCACCTT ATCTGGCTAA TTATCCAGGT 
TTTCCAGAGA ATGGAACTCC TGGGCCCCCA ATCTCGAGAT TCCCTCTGGA GGAATCTGCT 
CCCCCGGGAC CCCGCCCACT ACCCTGGCCT CCAGGCAATG ATGAAGCTGC CAAGATGCAG 
GCTCCACCGC CCAAGAAGGA ACCCTCGAAG GAGGAACCGC CACAGCTGTC CGGGCCAGAA 
GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA GGCCCACCAC CACCACGGAG AGAGAATCGT 
ACTGAGACTC GCTGGGGCCC CCGACCAGGC AGTTGTCGCC GTGGAATCCC TCCAGAGGAG 
CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT ATAAAGAAGC CACCACCACC TGTGAAAGTA 
GAGGAGCTGC CTCCTAAGTC CCTAGAACAG GGGGATGAGA CCCCCAAGGT CCCAAAGCCA 
GATGCACTCA AGACAGCTAA GGGCAAGGTG GGCCCCAAAG AAACCCCACC AGGTGGAAAC 
CTGTCTCCTG CACCTAGGCT TCGGCGGGAC TATTCCTATG AAAGAGTAGG TCCTACATCT 
TGCCGTGGTC GGGGCAGAGG CGAGTATTTT GCCAGAGGAA GGGGTTTTCG GGGGACCTAT 
GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT GAATTCCGAA GTTACCGAGA GTTCCGAGGA 
GACGATGGGC GTGGGGGTGG TTCAGGCGGA ACAAACCATC CTTCTGCTCC CCGAGGCCGC 
ACTGCCAGTG AGACACGAAG TGAGGGTTCA GAATATGAAG AAATCCCCAA GAGACGCCGC 
CAGCGTGGCT CTGAAACAGG CAGTGAGACT CATGAGAGTG ACCTGGCCCC CTCTGACAAG 
GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT GGTCAGGTCC CTCTTGCCCC CCCACAACCA 
GGAGCTCCTC CATCACCAGC CCCAGCCCGA TTCTCCACTG CCCGAGGTGG TCGTGTCTTC 
ACTCCAAGAG GAGTACCATC ACGCCGTGGC CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC 
TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG GTTCCAAAGA AACCTCCAAC AGGTCCTTTG 
CCTCCAAGTA AAGAACCTTT GAAAGAGAAG CTGATCTCAG GGCCTCTGTC CCCCATGTCT 
CGAGCTGGCA ACATGGGTGT AGGCATGGAA GATGGGGAGC GACCCCGACG GAGGCGACAT 
GGCAGGGCTC AACAGCAGGA CAAACCACCT CGTTTCCGAA GGCTGAAACA AGAACGTGAA 
AACGCTGCCA GAGGAGCTGA TGGCAAGCCC CCTTCCCTAA CCCTTGCAGC CTCTACCCCT 
GGACCTGAGG AGACACTAAC AGCAGCCACA GTTCCCCCAC CCCCTCGCCG CACAGCTGCT 
AAGTCTCCTG ATCTGTCAAA CCAGAACTCA GACCAAGCCA ATGAGGAATG GGAGACTGCA 
TCAGAGAGCA GCGACTTTGC CAGTGAGCGC CGGGGTGACA AGGAGACTCC CCCAGCAGCA 
CTTATGACCT CTAAAGCTGT GGGGACTCCT GGGGCCAATG CAGGTGGGGC TGGACCAGGC 
ATTTCAGCCA TGTCTCGTGG AGACCTGAGC CAACGAGCCA AGGATTTGAG CAAGCGGAGC 
TTCTCAAGTC AGCGGCCAGG CATGGATCGT CAAAACCGGC GCCCAGGCAC TGGGGGCAAG 
ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT GGTGGTGCAG GCCCTGGGGG ACGGACCGGC 
CCAGGACGAG GGGATAAGAG GAGCTGGCCC TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG 
GAGCGACCTC CGGGACTTCC TCTGCCTCCG CCACCCCCCA GCAGTTCTGC TGTCTTCCGC 
CTGGACCAAG TTATCCATAG CAACCCTGCT GGAATCCAAC AGGCTCTGGC CCAACTTAGC 
AGCCGCCAAG GAAATGTAAC TGCACCAGGG GGGCACCCAA GGCCTAAGCC TGGGCCTCCC 
CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC CCAACCCGTT ATGACCCCCC AAGGGCCAGC 
AGCGCTATCA GTTCTGACCC CCACTTCGAG GAGCCAGGGC CGATGGTGAG GGGGGTGGGC 
GGGACTCCCC GGGACTCTGC CGGGGTTAAT CCCTTCCCCC CGAAACGGCG GGAGCGGCCT 
CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA ACTGTGCCAG CTTCTCATAG CTCTGGATTC 
TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT CAGGAAGAAT CCAGAGATTC AGGCACAGAG 
GCCCTAACTC CCCACATCTG GAATCGTTTA CATACTGCCA CAAGCCGAAA GAGTTACCAG 
CCTGGCTCCA TCGAGCCCTG GATGGAGCCC CTGAGTCCCT TTGAGGATGT GGCAGGCACA 
GAGATGAGTC AGTCTGACAG TGGAGTGGAC CTGAGTGGAG ATTCTCAGGT GTCATCAGGT 
CCCTGCAGTC AGCGAAGTTC CCCTGATGGA GGACTTAAGG GGTCAGCAGA GGGACCCCCC 
AAGCGGCCTG GAGGACCCTC ACCCCTGAAT GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT 
GAACCCTCTG AACCTCCTAG GAGACGGCCA CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG 
CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT CCCATAGGCA CAGAACGATC ACAGCGCACA 
GATCGAGGCC CAGAGCCAGG CCCTCTCCGA CCAGCCCACC GACCGGGCTC TCAAGTTGAG 
TTTGGCACTA CCAACAAGGA CTCAGACTTG TGCCTAGTGG TCGGAGACAC TTTAAAAGGA 
GAAAAGGAGT TGGTGGCATC AGCCACAGAG GCCGTTCCCA TATCCAGAGA CTGGGAGCTG 
CTCCCTAGTG CTTCTACTTC AGCTGAGCCA CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT 
GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT CCGTACCCTG AGGTCTTCTA TGGCAGCCCT 
GGACCTCCTA ATTCCCAGGT CTCTGGGGGT GCACCCATCG ACTCTCAGTT ACATCCCAAC 
AGTGGAGGCT TCCGTCCTGG GACACCCTCA TTGCACCAGT ACAGGTCACA GCCTCTGTAT 
CTGCCCCCAG GTCCAGCCCC TCCCTCGGCA CTGCTTTCTG GGGTAGCTCT CAAGGGCCAG 
TTTCTGGATT TCTCAGCACT GCAAGCCACA GAGCTGGGGA AGTTGCCTGC TGGGGGAGTT 
CTCTACCCTC CGCCATCCTT CCTCTACTCT GCAGCTTTCT GCCCCAGCCC TTTGCCTGAC 
CCACCCTTGC TTCAGGTACG CCAGGATCTG CCATCCCCTT CAGATTTTTA CTCTACTCCT 
CTGCAGCCTG GTGGCCAGAG TGGCTTCCTT CCTTCGGGAG CCCCTGCTCA GCAGATGCTT 
CTACCTGTGG TAGACTCACA GCTGCCAGTG GTGAACTTTG GCTCCCTGCC ACCAGCGCCA 
CCCCCTGCTC CACCCCCCCT TTCTCTGTTA CCTGTGGGTC CTGCTCTCCA GCCCCCCAAC 
CTAGCTGTGC GGCCCCCACC TGCTCCTGCT GCTCGGGTCT TACCTTCACC CGCTAGACCC 
TTCGCCCCTA GCTTGGGGCG AGCAGAGCTG CACCCAGTAG AACTAAAGCC TTTCCAGGAT 
TACCGAAAGC TGAGCAGCAA CCTTGGGGGT CCTGGGTCGT CACGGACTCC CCCATCTGGA 
AGGCCCTTCT CTGGTCTCAA TTCCCGTCTC AAGGCCCCAC CTTCCACCTA CAGTGGAGTA 
TTCCGAACTC AGCGCATTGA CCTCTACCAG CAGGCTTCCC CACCAGATGC CCTGCGCTGG 
ATGCCGAAGC CTTGGGAACG GGCAGGGCCA CCTTCTCGAG AAGGGCCTCC CCGAAGAGCA 
GAGGAGCCTG GGTCCCGGGG GGAGAAGGAG CCTGGATTGC CCCCACCCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185973.1
CDS	207..6680
Translation	MSDRSGPTAKGKDGKKYSSLNLFDTYKGKSLEIQKPAVAPRHGLQSLGKVAIARRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQSDPKSSDASTAQPPESQPLPASQTPASNQPKRPPTAPENTPSVPSGVKSWAQASVTHGAHGDGGRASNLLSRFSREEFPTLQAAGDQDKAAKERESAEQSSGPGPSLRPQNSTTWRDGGGRGPDDLEGPDSKLHHGHDPRGGLQPSGPPQFPPYRGMMPPFMYPPYLPFPPPYGPQGPYRYPTPDGPSRFPRVAGPRGSGPPMRLVEPVGRPSILKEDNLKEFDQLDQENDDGWAGAHEEVDYTEKLKFSDEEDGRDSDEEGAEGHKDSQSAAAEEPETDGKKGTSPGSELPPPKTAWTENARPSETEPAPPTPKPPPPPPHRGPVGNWGPPGDYPDRGGPPCKPPAPEDEDEAWRQRRKQSSSEISLAVERARRRREEEERRMQEERRAACAEKLKRLDEKFGAPDKRLKAEPAAPPVTPAAPALPPVVPKEIPAAPALPPTPTPTPEKEPEEPAQAPPVQAAPSPGVAPVPTLVSGGGCTANSNSSGSFEASPVEPQLPSKEGPEPPEEVPPPTTPPAPKMEPKGDGVGSTRQPPSQGLGYPKYQKSLPPRFQRQQQEQLLKQQQQQQQWQQQQQGTAPPAPVPPSPPQPVTLGAVPAPQAPPPPPKALYPGALGRPPPMPPMNFDPRWMMIPPYVDPRLLQGRPPLDFYPPGVHPSGLVPRERSDSGGSSSEPFERHAPPLLRERGTPPVDPKLAWVGDVFTTTPTDPRPLTSPLRQAADEEEKSMRSETPPVPPPPPYLANYPGFPENGTPGPPISRFPLEESAPPGPRPLPWPPGNDEAAKMQAPPPKKEPSKEEPPQLSGPEAGRKPARGGQGPPPPRRENRTETRWGPRPGSCRRGIPPEEPGVPPRRAGPIKKPPPPVKVEELPPKSLEQGDETPKVPKPDALKTAKGKVGPKETPPGGNLSPAPRLRRDYSYERVGPTSCRGRGRGEYFARGRGFRGTYGGRGRGARSREFRSYREFRGDDGRGGGSGGTNHPSAPRGRTASETRSEGSEYEEIPKRRRQRGSETGSETHESDLAPSDKEAPPPKEGVLGQVPLAPPQPGAPPSPAPARFSTARGGRVFTPRGVPSRRGRGGGRPPPVCSGWSPPAKSLVPKKPPTGPLPPSKEPLKEKLISGPLSPMSRAGNMGVGMEDGERPRRRRHGRAQQQDKPPRFRRLKQERENAARGADGKPPSLTLAASTPGPEETLTAATVPPPPRRTAAKSPDLSNQNSDQANEEWETASESSDFASERRGDKETPPAALMTSKAVGTPGANAGGAGPGISAMSRGDLSQRAKDLSKRSFSSQRPGMDRQNRRPGTGGKTGSGGGSSGGGGAGPGGRTGPGRGDKRSWPSPKNRSRPPEERPPGLPLPPPPPSSSAVFRLDQVIHSNPAGIQQALAQLSSRQGNVTAPGGHPRPKPGPPQAPQGSSPRPPTRYDPPRASSAISSDPHFEEPGPMVRGVGGTPRDSAGVNPFPPKRRERPPRKPELLQEETVPASHSSGFLGSKPEVPGPQEESRDSGTEALTPHIWNRLHTATSRKSYQPGSIEPWMEPLSPFEDVAGTEMSQSDSGVDLSGDSQVSSGPCSQRSSPDGGLKGSAEGPPKRPGGPSPLNAVPGESASGSEPSEPPRRRPPASHEGERKELPREQPLPPGPIGTERSQRTDRGPEPGPLRPAHRPGSQVEFGTTNKDSDLCLVVGDTLKGEKELVASATEAVPISRDWELLPSASTSAEPQPKSLGSGQCVPEPSPSGQRPYPEVFYGSPGPPNSQVSGGAPIDSQLHPNSGGFRPGTPSLHQYRSQPLYLPPGPAPPSALLSGVALKGQFLDFSALQATELGKLPAGGVLYPPPSFLYSAAFCPSPLPDPPLLQVRQDLPSPSDFYSTPLQPGGQSGFLPSGAPAQQMLLPVVDSQLPVVNFGSLPPAPPPAPPPLSLLPVGPALQPPNLAVRPPPAPAARVLPSPARPFAPSLGRAELHPVELKPFQDYRKLSSNLGGPGSSRTPPSGRPFSGLNSRLKAPPSTYSGVFRTQRIDLYQQASPPDALRWMPKPWERAGPPSREGPPRRAEEPGSRGEKEPGLPPPR

Target ORF information:

RefSeq Version	NM_001199044.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199044.1

ORF Insert Sequence:

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGGCAAGTCC TTAGAGATCC AGAAACCCGC TGTTGCCCCT 
CGACATGGCC TGCAGAGTCT CGGAAAAGTT GCCATTGCCC GGCGCATGCC ACCCCCAGCC 
AACCTTCCAA GCCTGAAAGC CGAGAACAAA GGCAATGACC CCAATGTCTC ACTAGTGCCG 
AAAGACGGAA CAGGATGGGC AAGCAAACAG GAGCAGTCCG ACCCCAAGAG TTCCGATGCC 
TCAACCGCTC AGCCGCCGGA ATCGCAGCCA CTGCCGGCTT CACAGACGCC TGCCTCCAAC 
CAGCCGAAAC GACCCCCAAC AGCCCCCGAG AACACTCCTT CGGTTCCAAG CGGGGTAAAG 
TCCTGGGCAC AAGCCAGTGT CACCCATGGA GCCCATGGAG ATGGTGGAAG GGCATCAAAC 
CTACTGTCGC GATTCTCTCG AGAGGAATTT CCGACCCTGC AGGCGGCTGG AGACCAGGAC 
AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA CAGTCGTCTG GGCCCGGACC AAGCCTCCGC 
CCCCAAAATT CTACAACTTG GAGGGACGGA GGTGGGCGTG GCCCTGATGA CCTGGAGGGC 
CCGGACTCCA AACTTCATCA TGGCCATGAT CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA 
CCCCAGTTCC CTCCCTACCG CGGGATGATG CCGCCCTTTA TGTATCCCCC ATATCTCCCA 
TTCCCTCCGC CCTATGGACC CCAGGGGCCT TATAGATACC CGACTCCTGA TGGGCCCAGC 
CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC TCAGGGCCAC CAATGCGCCT TGTAGAGCCT 
GTGGGTCGGC CTTCTATTCT TAAAGAGGAT AATCTTAAAG AGTTTGACCA ACTGGACCAG 
GAAAATGATG ATGGTTGGGC AGGGGCCCAT GAAGAGGTTG ACTATACTGA AAAGCTCAAA 
TTCAGCGATG AGGAAGATGG ACGAGACTCC GACGAGGAGG GAGCTGAGGG CCACAAGGAT 
TCCCAGTCAG CTGCTGCTGA GGAACCTGAG ACAGATGGCA AAAAGGGCAC CTCCCCTGGC 
AGCGAATTGC CCCCTCCCAA GACAGCCTGG ACAGAGAATG CTCGGCCTTC AGAGACAGAG 
CCAGCTCCTC CTACCCCAAA GCCTCCCCCA CCTCCACCTC ACCGGGGGCC AGTCGGGAAC 
TGGGGGCCCC CTGGGGACTA CCCAGATCGA GGGGGTCCTC CCTGCAAACC CCCAGCACCT 
GAGGATGAGG ATGAGGCATG GCGGCAGCGT CGGAAGCAAT CTTCTTCAGA GATCTCCCTG 
GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA GAGGAAGAGC GACGCATGCA GGAGGAGCGC 
CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT CTTGATGAAA AGTTTGGGGC ACCTGACAAG 
CGGCTCAAGG CAGAGCCTGC AGCCCCACCT GTTACCCCTG CTGCCCCAGC TCTACCACCT 
GTAGTCCCCA AAGAAATTCC TGCAGCTCCA GCTCTGCCAC CAACACCTAC CCCAACACCA 
GAGAAAGAAC CTGAAGAGCC GGCACAAGCC CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG 
GTGGCTCCAG TTCCCACTCT GGTGAGTGGG GGTGGTTGCA CTGCCAACAG CAACAGCAGT 
GGCAGCTTCG AAGCCAGCCC AGTGGAACCC CAACTGCCTT CAAAAGAGGG TCCTGAACCA 
CCAGAAGAGG TTCCTCCACC TACCACACCC CCAGCCCCAA AGATGGAACC CAAAGGTGAT 
GGGGTTGGTT CTACTCGCCA GCCCCCCAGT CAGGGCTTGG GCTACCCCAA ATACCAGAAG 
TCATTGCCTC CTCGCTTCCA GCGGCAGCAA CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG 
CAACAGCAGT GGCAGCAGCA GCAACAAGGC ACTGCCCCTC CTGCCCCAGT GCCCCCATCA 
CCACCACAAC CTGTGACCCT GGGAGCTGTA CCTGCCCCAC AAGCACCGCC CCCACCTCCC 
AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG CCCCCACCAA TGCCTCCAAT GAACTTTGAC 
CCCCGCTGGA TGATGATTCC TCCTTACGTG GACCCTCGGC TTCTCCAGGG CCGGCCCCCT 
CTGGACTTCT ACCCTCCTGG TGTGCATCCC TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC 
AGTGGGGGCT CAAGCTCAGA GCCATTTGAA CGCCATGCAC CCCCTCTGTT GCGGGAAAGG 
GGCACCCCAC CAGTGGACCC CAAGTTGGCC TGGGTTGGAG ATGTCTTTAC CACCACACCC 
ACTGATCCTC GTCCTCTTAC CTCACCACTG CGCCAGGCAG CAGATGAGGA GGAGAAGAGT 
ATGAGGAGCG AGACTCCTCC GGTACCCCCG CCCCCACCTT ATCTGGCTAA TTATCCAGGT 
TTTCCAGAGA ATGGAACTCC TGGGCCCCCA ATCTCGAGAT TCCCTCTGGA GGAATCTGCT 
CCCCCGGGAC CCCGCCCACT ACCCTGGCCT CCAGGCAATG ATGAAGCTGC CAAGATGCAG 
GCTCCACCGC CCAAGAAGGA ACCCTCGAAG GAGGAACCGC CACAGCTGTC CGGGCCAGAA 
GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA GGCCCACCAC CACCACGGAG AGAGAATCGT 
ACTGAGACTC GCTGGGGCCC CCGACCAGGC AGTTGTCGCC GTGGAATCCC TCCAGAGGAG 
CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT ATAAAGAAGC CACCACCACC TGTGAAAGTA 
GAGGAGCTGC CTCCTAAGTC CCTAGAACAG GGGGATGAGA CCCCCAAGGT CCCAAAGCCA 
GATGCACTCA AGACAGCTAA GGGCAAGGTG GGCCCCAAAG AAACCCCACC AGGTGGAAAC 
CTGTCTCCTG CACCTAGGCT TCGGCGGGAC TATTCCTATG AAAGAGTAGG TCCTACATCT 
TGCCGTGGTC GGGGCAGAGG CGAGTATTTT GCCAGAGGAA GGGGTTTTCG GGGGACCTAT 
GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT GAATTCCGAA GTTACCGAGA GTTCCGAGGA 
GACGATGGGC GTGGGGGTGG TTCAGGCGGA ACAAACCATC CTTCTGCTCC CCGAGGCCGC 
ACTGCCAGTG AGACACGAAG TGAGGGTTCA GAATATGAAG AAATCCCCAA GAGACGCCGC 
CAGCGTGGCT CTGAAACAGG CAGTGAGACT CATGAGAGTG ACCTGGCCCC CTCTGACAAG 
GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT GGTCAGGTCC CTCTTGCCCC CCCACAACCA 
GGAGCTCCTC CATCACCAGC CCCAGCCCGA TTCTCCACTG CCCGAGGTGG TCGTGTCTTC 
ACTCCAAGAG GAGTACCATC ACGCCGTGGC CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC 
TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG GTTCCAAAGA AACCTCCAAC AGGTCCTTTG 
CCTCCAAGTA AAGAACCTTT GAAAGAGAAG CTGATCTCAG GGCCTCTGTC CCCCATGTCT 
CGAGCTGGCA ACATGGGTGT AGGCATGGAA GATGGGGAGC GACCCCGACG GAGGCGACAT 
GGCAGGGCTC AACAGCAGGA CAAACCACCT CGTTTCCGAA GGCTGAAACA AGAACGTGAA 
AACGCTGCCA GAGGAGCTGA TGGCAAGCCC CCTTCCCTAA CCCTTGCAGC CTCTACCCCT 
GGACCTGAGG AGACACTAAC AGCAGCCACA GTTCCCCCAC CCCCTCGCCG CACAGCTGCT 
AAGTCTCCTG ATCTGTCAAA CCAGAACTCA GACCAAGCCA ATGAGGAATG GGAGACTGCA 
TCAGAGAGCA GCGACTTTGC CAGTGAGCGC CGGGGTGACA AGGAGACTCC CCCAGCAGCA 
CTTATGACCT CTAAAGCTGT GGGGACTCCT GGGGCCAATG CAGGTGGGGC TGGACCAGGC 
ATTTCAGCCA TGTCTCGTGG AGACCTGAGC CAACGAGCCA AGGATTTGAG CAAGCGGAGC 
TTCTCAAGTC AGCGGCCAGG CATGGATCGT CAAAACCGGC GCCCAGGCAC TGGGGGCAAG 
ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT GGTGGTGCAG GCCCTGGGGG ACGGACCGGC 
CCAGGACGAG GGGATAAGAG GAGCTGGCCC TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG 
GAGCGACCTC CGGGACTTCC TCTGCCTCCG CCACCCCCCA GCAGTTCTGC TGTCTTCCGC 
CTGGACCAAG TTATCCATAG CAACCCTGCT GGAATCCAAC AGGCTCTGGC CCAACTTAGC 
AGCCGCCAAG GAAATGTAAC TGCACCAGGG GGGCACCCAA GGCCTAAGCC TGGGCCTCCC 
CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC CCAACCCGTT ATGACCCCCC AAGGGCCAGC 
AGCGCTATCA GTTCTGACCC CCACTTCGAG GAGCCAGGGC CGATGGTGAG GGGGGTGGGC 
GGGACTCCCC GGGACTCTGC CGGGGTTAAT CCCTTCCCCC CGAAACGGCG GGAGCGGCCT 
CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA ACTGTGCCAG CTTCTCATAG CTCTGGATTC 
TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT CAGGAAGAAT CCAGAGATTC AGGCACAGAG 
GCCCTAACTC CCCACATCTG GAATCGTTTA CATACTGCCA CAAGCCGAAA GAGTTACCAG 
CCTGGCTCCA TCGAGCCCTG GATGGAGCCC CTGAGTCCCT TTGAGGATGT GGCAGGCACA 
GAGATGAGTC AGTCTGACAG TGGAGTGGAC CTGAGTGGAG ATTCTCAGGT GTCATCAGGT 
CCCTGCAGTC AGCGAAGTTC CCCTGATGGA GGACTTAAGG GGTCAGCAGA GGGACCCCCC 
AAGCGGCCTG GAGGACCCTC ACCCCTGAAT GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT 
GAACCCTCTG AACCTCCTAG GAGACGGCCA CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG 
CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT CCCATAGGCA CAGAACGATC ACAGCGCACA 
GATCGAGGCC CAGAGCCAGG CCCTCTCCGA CCAGCCCACC GACCGGGCTC TCAAGTTGAG 
TTTGGCACTA CCAACAAGGA CTCAGACTTG TGCCTAGTGG TCGGAGACAC TTTAAAAGGA 
GAAAAGGAGT TGGTGGCATC AGCCACAGAG GCCGTTCCCA TATCCAGAGA CTGGGAGCTG 
CTCCCTAGTG CTTCTACTTC AGCTGAGCCA CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT 
GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT CCGTACCCTG AGGTCTTCTA TGGCAGCCCT 
GGACCTCCTA ATTCCCAGGT CTCTGGGGGT GCACCCATCG ACTCTCAGTT ACATCCCAAC 
AGTGGAGGCT TCCGTCCTGG GACACCCTCA TTGCACCAGT ACAGGTCACA GCCTCTGTAT 
CTGCCCCCAG GTCCAGCCCC TCCCTCGGCA CTGCTTTCTG GGGTAGCTCT CAAGGGCCAG 
TTTCTGGATT TCTCAGCACT GCAAGCCACA GAGCTGGGGA AGTTGCCTGC TGGGGGAGTT 
CTCTACCCTC CGCCATCCTT CCTCTACTCT GCAGCTTTCT GCCCCAGCCC TTTGCCTGAC 
CCACCCTTGC TTCAGGTACG CCAGGATCTG CCATCCCCTT CAGATTTTTA CTCTACTCCT 
CTGCAGCCTG GTGGCCAGAG TGGCTTCCTT CCTTCGGGAG CCCCTGCTCA GCAGATGCTT 
CTACCTGTGG TAGACTCACA GCTGCCAGTG GTGAACTTTG GCTCCCTGCC ACCAGCGCCA 
CCCCCTGCTC CACCCCCCCT TTCTCTGTTA CCTGTGGGTC CTGCTCTCCA GCCCCCCAAC 
CTAGCTGTGC GGCCCCCACC TGCTCCTGCT GCTCGGGTCT TACCTTCACC CGCTAGACCC 
TTCGCCCCTA GCTTGGGGCG AGCAGAGCTG CACCCAGTAG AACTAAAGCC TTTCCAGGAT 
TACCGAAAGC TGAGCAGCAA CCTTGGGGGT CCTGGGTCGT CACGGACTCC CCCATCTGGA 
AGGCCCTTCT CTGGTCTCAA TTCCCGTCTC AAGGCCCCAC CTTCCACCTA CAGTGGAGTA 
TTCCGAACTC AGCGCATTGA CCTCTACCAG CAGGCTTCCC CACCAGATGC CCTGCGCTGG 
ATGCCGAAGC CTTGGGAACG GGCAGGGCCA CCTTCTCGAG AAGGGCCTCC CCGAAGAGCA 
GAGGAGCCTG GGTCCCGGGG GGAGAAGGAG CCTGGATTGC CCCCACCCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prrc2a ( XM_006524620.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524683.1 Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X1, mRNA.

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CloneID	OMu15568
Clone ID Related Accession (Same CDS sequence)	XM_006524620.3 , XM_006524620.4 , NM_020027.3
Accession Version	XM_006524620.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein PRRC2A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524620.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGGCAAGTCC TTAGAGATCC AGAAACCCGC TGTTGCCCCT 
CGACATGGCC TGCAGAGTCT CGGAAAAGTT GCCATTGCCC GGCGCATGCC ACCCCCAGCC 
AACCTTCCAA GCCTGAAAGC CGAGAACAAA GGCAATGACC CCAATGTCTC ACTAGTGCCG 
AAAGACGGAA CAGGATGGGC AAGCAAACAG GAGCAGTCCG ACCCCAAGAG TTCCGATGCC 
TCAACCGCTC AGCCGCCGGA ATCGCAGCCA CTGCCGGCTT CACAGACGCC TGCCTCCAAC 
CAGCCGAAAC GACCCCCAAC AGCCCCCGAG AACACTCCTT CGGTTCCAAG CGGGGTAAAG 
TCCTGGGCAC AAGCCAGTGT CACCCATGGA GCCCATGGAG ATGGTGGAAG GGCATCAAAC 
CTACTGTCGC GATTCTCTCG AGAGGAATTT CCGACCCTGC AGGCGGCTGG AGACCAGGAC 
AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA CAGTCGTCTG GGCCCGGACC AAGCCTCCGC 
CCCCAAAATT CTACAACTTG GAGGGACGGA GGTGGGCGTG GCCCTGATGA CCTGGAGGGC 
CCGGACTCCA AACTTCATCA TGGCCATGAT CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA 
CCCCAGTTCC CTCCCTACCG CGGGATGATG CCGCCCTTTA TGTATCCCCC ATATCTCCCA 
TTCCCTCCGC CCTATGGACC CCAGGGGCCT TATAGATACC CGACTCCTGA TGGGCCCAGC 
CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC TCAGGGCCAC CAATGCGCCT TGTAGAGCCT 
GTGGGTCGGC CTTCTATTCT TAAAGAGGAT AATCTTAAAG AGTTTGACCA ACTGGACCAG 
GAAAATGATG ATGGTTGGGC AGGGGCCCAT GAAGAGGTTG ACTATACTGA AAAGCTCAAA 
TTCAGCGATG AGGAAGATGG ACGAGACTCC GACGAGGAGG GAGCTGAGGG CCACAAGGAT 
TCCCAGTCAG CTGCTGCTGA GGAACCTGAG ACAGATGGCA AAAAGGGCAC CTCCCCTGGC 
AGCGAATTGC CCCCTCCCAA GACAGCCTGG ACAGAGAATG CTCGGCCTTC AGAGACAGAG 
CCAGCTCCTC CTACCCCAAA GCCTCCCCCA CCTCCACCTC ACCGGGGGCC AGTCGGGAAC 
TGGGGGCCCC CTGGGGACTA CCCAGATCGA GGGGGTCCTC CCTGCAAACC CCCAGCACCT 
GAGGATGAGG ATGAGGCATG GCGGCAGCGT CGGAAGCAAT CTTCTTCAGA GATCTCCCTG 
GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA GAGGAAGAGC GACGCATGCA GGAGGAGCGC 
CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT CTTGATGAAA AGTTTGGGGC ACCTGACAAG 
CGGCTCAAGG CAGAGCCTGC AGCCCCACCT GTTACCCCTG CTGCCCCAGC TCTACCACCT 
GTAGTCCCCA AAGAAATTCC TGCAGCTCCA GCTCTGCCAC CAACACCTAC CCCAACACCA 
GAGAAAGAAC CTGAAGAGCC GGCACAAGCC CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG 
GTGGCTCCAG TTCCCACTCT GGTGAGTGGG GGTGGTTGCA CTGCCAACAG CAACAGCAGT 
GGCAGCTTCG AAGCCAGCCC AGTGGAACCC CAACTGCCTT CAAAAGAGGG TCCTGAACCA 
CCAGAAGAGG TTCCTCCACC TACCACACCC CCAGCCCCAA AGATGGAACC CAAAGGTGAT 
GGGGTTGGTT CTACTCGCCA GCCCCCCAGT CAGGGCTTGG GCTACCCCAA ATACCAGAAG 
TCATTGCCTC CTCGCTTCCA GCGGCAGCAA CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG 
CAACAGCAGT GGCAGCAGCA GCAACAAGGC ACTGCCCCTC CTGCCCCAGT GCCCCCATCA 
CCACCACAAC CTGTGACCCT GGGAGCTGTA CCTGCCCCAC AAGCACCGCC CCCACCTCCC 
AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG CCCCCACCAA TGCCTCCAAT GAACTTTGAC 
CCCCGCTGGA TGATGATTCC TCCTTACGTG GACCCTCGGC TTCTCCAGGG CCGGCCCCCT 
CTGGACTTCT ACCCTCCTGG TGTGCATCCC TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC 
AGTGGGGGCT CAAGCTCAGA GCCATTTGAA CGCCATGCAC CCCCTCTGTT GCGGGAAAGG 
GGCACCCCAC CAGTGGACCC CAAGTTGGCC TGGGTTGGAG ATGTCTTTAC CACCACACCC 
ACTGATCCTC GTCCTCTTAC CTCACCACTG CGCCAGGCAG CAGATGAGGA GGAGAAGAGT 
ATGAGGAGCG AGACTCCTCC GGTACCCCCG CCCCCACCTT ATCTGGCTAA TTATCCAGGT 
TTTCCAGAGA ATGGAACTCC TGGGCCCCCA ATCTCGAGAT TCCCTCTGGA GGAATCTGCT 
CCCCCGGGAC CCCGCCCACT ACCCTGGCCT CCAGGCAATG ATGAAGCTGC CAAGATGCAG 
GCTCCACCGC CCAAGAAGGA ACCCTCGAAG GAGGAACCGC CACAGCTGTC CGGGCCAGAA 
GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA GGCCCACCAC CACCACGGAG AGAGAATCGT 
ACTGAGACTC GCTGGGGCCC CCGACCAGGC AGTTGTCGCC GTGGAATCCC TCCAGAGGAG 
CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT ATAAAGAAGC CACCACCACC TGTGAAAGTA 
GAGGAGCTGC CTCCTAAGTC CCTAGAACAG GGGGATGAGA CCCCCAAGGT CCCAAAGCCA 
GATGCACTCA AGACAGCTAA GGGCAAGGTG GGCCCCAAAG AAACCCCACC AGGTGGAAAC 
CTGTCTCCTG CACCTAGGCT TCGGCGGGAC TATTCCTATG AAAGAGTAGG TCCTACATCT 
TGCCGTGGTC GGGGCAGAGG CGAGTATTTT GCCAGAGGAA GGGGTTTTCG GGGGACCTAT 
GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT GAATTCCGAA GTTACCGAGA GTTCCGAGGA 
GACGATGGGC GTGGGGGTGG TTCAGGCGGA ACAAACCATC CTTCTGCTCC CCGAGGCCGC 
ACTGCCAGTG AGACACGAAG TGAGGGTTCA GAATATGAAG AAATCCCCAA GAGACGCCGC 
CAGCGTGGCT CTGAAACAGG CAGTGAGACT CATGAGAGTG ACCTGGCCCC CTCTGACAAG 
GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT GGTCAGGTCC CTCTTGCCCC CCCACAACCA 
GGAGCTCCTC CATCACCAGC CCCAGCCCGA TTCTCCACTG CCCGAGGTGG TCGTGTCTTC 
ACTCCAAGAG GAGTACCATC ACGCCGTGGC CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC 
TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG GTTCCAAAGA AACCTCCAAC AGGTCCTTTG 
CCTCCAAGTA AAGAACCTTT GAAAGAGAAG CTGATCTCAG GGCCTCTGTC CCCCATGTCT 
CGAGCTGGCA ACATGGGTGT AGGCATGGAA GATGGGGAGC GACCCCGACG GAGGCGACAT 
GGCAGGGCTC AACAGCAGGA CAAACCACCT CGTTTCCGAA GGCTGAAACA AGAACGTGAA 
AACGCTGCCA GAGGAGCTGA TGGCAAGCCC CCTTCCCTAA CCCTTGCAGC CTCTACCCCT 
GGACCTGAGG AGACACTAAC AGCAGCCACA GTTCCCCCAC CCCCTCGCCG CACAGCTGCT 
AAGTCTCCTG ATCTGTCAAA CCAGAACTCA GACCAAGCCA ATGAGGAATG GGAGACTGCA 
TCAGAGAGCA GCGACTTTGC CAGTGAGCGC CGGGGTGACA AGGAGACTCC CCCAGCAGCA 
CTTATGACCT CTAAAGCTGT GGGGACTCCT GGGGCCAATG CAGGTGGGGC TGGACCAGGC 
ATTTCAGCCA TGTCTCGTGG AGACCTGAGC CAACGAGCCA AGGATTTGAG CAAGCGGAGC 
TTCTCAAGTC AGCGGCCAGG CATGGATCGT CAAAACCGGC GCCCAGGCAC TGGGGGCAAG 
ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT GGTGGTGCAG GCCCTGGGGG ACGGACCGGC 
CCAGGACGAG GGGATAAGAG GAGCTGGCCC TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG 
GAGCGACCTC CGGGACTTCC TCTGCCTCCG CCACCCCCCA GCAGTTCTGC TGTCTTCCGC 
CTGGACCAAG TTATCCATAG CAACCCTGCT GGAATCCAAC AGGCTCTGGC CCAACTTAGC 
AGCCGCCAAG GAAATGTAAC TGCACCAGGG GGGCACCCAA GGCCTAAGCC TGGGCCTCCC 
CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC CCAACCCGTT ATGACCCCCC AAGGGCCAGC 
AGCGCTATCA GTTCTGACCC CCACTTCGAG GAGCCAGGGC CGATGGTGAG GGGGGTGGGC 
GGGACTCCCC GGGACTCTGC CGGGGTTAAT CCCTTCCCCC CGAAACGGCG GGAGCGGCCT 
CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA ACTGTGCCAG CTTCTCATAG CTCTGGATTC 
TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT CAGGAAGAAT CCAGAGATTC AGGCACAGAG 
GCCCTAACTC CCCACATCTG GAATCGTTTA CATACTGCCA CAAGCCGAAA GAGTTACCAG 
CCTGGCTCCA TCGAGCCCTG GATGGAGCCC CTGAGTCCCT TTGAGGATGT GGCAGGCACA 
GAGATGAGTC AGTCTGACAG TGGAGTGGAC CTGAGTGGAG ATTCTCAGGT GTCATCAGGT 
CCCTGCAGTC AGCGAAGTTC CCCTGATGGA GGACTTAAGG GGTCAGCAGA GGGACCCCCC 
AAGCGGCCTG GAGGACCCTC ACCCCTGAAT GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT 
GAACCCTCTG AACCTCCTAG GAGACGGCCA CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG 
CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT CCCATAGGCA CAGAACGATC ACAGCGCACA 
GATCGAGGCC CAGAGCCAGG CCCTCTCCGA CCAGCCCACC GACCGGGCTC TCAAGTTGAG 
TTTGGCACTA CCAACAAGGA CTCAGACTTG TGCCTAGTGG TCGGAGACAC TTTAAAAGGA 
GAAAAGGAGT TGGTGGCATC AGCCACAGAG GCCGTTCCCA TATCCAGAGA CTGGGAGCTG 
CTCCCTAGTG CTTCTACTTC AGCTGAGCCA CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT 
GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT CCGTACCCTG AGGTCTTCTA TGGCAGCCCT 
GGACCTCCTA ATTCCCAGCA GGTCTCTGGG GGTGCACCCA TCGACTCTCA GTTACATCCC 
AACAGTGGAG GCTTCCGTCC TGGGACACCC TCATTGCACC AGTACAGGTC ACAGCCTCTG 
TATCTGCCCC CAGGTCCAGC CCCTCCCTCG GCACTGCTTT CTGGGGTAGC TCTCAAGGGC 
CAGTTTCTGG ATTTCTCAGC ACTGCAAGCC ACAGAGCTGG GGAAGTTGCC TGCTGGGGGA 
GTTCTCTACC CTCCGCCATC CTTCCTCTAC TCTGCAGCTT TCTGCCCCAG CCCTTTGCCT 
GACCCACCCT TGCTTCAGGT ACGCCAGGAT CTGCCATCCC CTTCAGATTT TTACTCTACT 
CCTCTGCAGC CTGGTGGCCA GAGTGGCTTC CTTCCTTCGG GAGCCCCTGC TCAGCAGATG 
CTTCTACCTG TGGTAGACTC ACAGCTGCCA GTGGTGAACT TTGGCTCCCT GCCACCAGCG 
CCACCCCCTG CTCCACCCCC CCTTTCTCTG TTACCTGTGG GTCCTGCTCT CCAGCCCCCC 
AACCTAGCTG TGCGGCCCCC ACCTGCTCCT GCTGCTCGGG TCTTACCTTC ACCCGCTAGA 
CCCTTCGCCC CTAGCTTGGG GCGAGCAGAG CTGCACCCAG TAGAACTAAA GCCTTTCCAG 
GATTACCGAA AGCTGAGCAG CAACCTTGGG GGTCCTGGGT CGTCACGGAC TCCCCCATCT 
GGAAGGCCCT TCTCTGGTCT CAATTCCCGT CTCAAGGCCC CACCTTCCAC CTACAGTGGA 
GTATTCCGAA CTCAGCGCAT TGACCTCTAC CAGCAGGCTT CCCCACCAGA TGCCCTGCGC 
TGGATGCCGA AGCCTTGGGA ACGGGCAGGG CCACCTTCTC GAGAAGGGCC TCCCCGAAGA 
GCAGAGGAGC CTGGGTCCCG GGGGGAGAAG GAGCCTGGAT TGCCCCCACC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524683.1
CDS	151..6627
Translation	MSDRSGPTAKGKDGKKYSSLNLFDTYKGKSLEIQKPAVAPRHGLQSLGKVAIARRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQSDPKSSDASTAQPPESQPLPASQTPASNQPKRPPTAPENTPSVPSGVKSWAQASVTHGAHGDGGRASNLLSRFSREEFPTLQAAGDQDKAAKERESAEQSSGPGPSLRPQNSTTWRDGGGRGPDDLEGPDSKLHHGHDPRGGLQPSGPPQFPPYRGMMPPFMYPPYLPFPPPYGPQGPYRYPTPDGPSRFPRVAGPRGSGPPMRLVEPVGRPSILKEDNLKEFDQLDQENDDGWAGAHEEVDYTEKLKFSDEEDGRDSDEEGAEGHKDSQSAAAEEPETDGKKGTSPGSELPPPKTAWTENARPSETEPAPPTPKPPPPPPHRGPVGNWGPPGDYPDRGGPPCKPPAPEDEDEAWRQRRKQSSSEISLAVERARRRREEEERRMQEERRAACAEKLKRLDEKFGAPDKRLKAEPAAPPVTPAAPALPPVVPKEIPAAPALPPTPTPTPEKEPEEPAQAPPVQAAPSPGVAPVPTLVSGGGCTANSNSSGSFEASPVEPQLPSKEGPEPPEEVPPPTTPPAPKMEPKGDGVGSTRQPPSQGLGYPKYQKSLPPRFQRQQQEQLLKQQQQQQQWQQQQQGTAPPAPVPPSPPQPVTLGAVPAPQAPPPPPKALYPGALGRPPPMPPMNFDPRWMMIPPYVDPRLLQGRPPLDFYPPGVHPSGLVPRERSDSGGSSSEPFERHAPPLLRERGTPPVDPKLAWVGDVFTTTPTDPRPLTSPLRQAADEEEKSMRSETPPVPPPPPYLANYPGFPENGTPGPPISRFPLEESAPPGPRPLPWPPGNDEAAKMQAPPPKKEPSKEEPPQLSGPEAGRKPARGGQGPPPPRRENRTETRWGPRPGSCRRGIPPEEPGVPPRRAGPIKKPPPPVKVEELPPKSLEQGDETPKVPKPDALKTAKGKVGPKETPPGGNLSPAPRLRRDYSYERVGPTSCRGRGRGEYFARGRGFRGTYGGRGRGARSREFRSYREFRGDDGRGGGSGGTNHPSAPRGRTASETRSEGSEYEEIPKRRRQRGSETGSETHESDLAPSDKEAPPPKEGVLGQVPLAPPQPGAPPSPAPARFSTARGGRVFTPRGVPSRRGRGGGRPPPVCSGWSPPAKSLVPKKPPTGPLPPSKEPLKEKLISGPLSPMSRAGNMGVGMEDGERPRRRRHGRAQQQDKPPRFRRLKQERENAARGADGKPPSLTLAASTPGPEETLTAATVPPPPRRTAAKSPDLSNQNSDQANEEWETASESSDFASERRGDKETPPAALMTSKAVGTPGANAGGAGPGISAMSRGDLSQRAKDLSKRSFSSQRPGMDRQNRRPGTGGKTGSGGGSSGGGGAGPGGRTGPGRGDKRSWPSPKNRSRPPEERPPGLPLPPPPPSSSAVFRLDQVIHSNPAGIQQALAQLSSRQGNVTAPGGHPRPKPGPPQAPQGSSPRPPTRYDPPRASSAISSDPHFEEPGPMVRGVGGTPRDSAGVNPFPPKRRERPPRKPELLQEETVPASHSSGFLGSKPEVPGPQEESRDSGTEALTPHIWNRLHTATSRKSYQPGSIEPWMEPLSPFEDVAGTEMSQSDSGVDLSGDSQVSSGPCSQRSSPDGGLKGSAEGPPKRPGGPSPLNAVPGESASGSEPSEPPRRRPPASHEGERKELPREQPLPPGPIGTERSQRTDRGPEPGPLRPAHRPGSQVEFGTTNKDSDLCLVVGDTLKGEKELVASATEAVPISRDWELLPSASTSAEPQPKSLGSGQCVPEPSPSGQRPYPEVFYGSPGPPNSQQVSGGAPIDSQLHPNSGGFRPGTPSLHQYRSQPLYLPPGPAPPSALLSGVALKGQFLDFSALQATELGKLPAGGVLYPPPSFLYSAAFCPSPLPDPPLLQVRQDLPSPSDFYSTPLQPGGQSGFLPSGAPAQQMLLPVVDSQLPVVNFGSLPPAPPPAPPPLSLLPVGPALQPPNLAVRPPPAPAARVLPSPARPFAPSLGRAELHPVELKPFQDYRKLSSNLGGPGSSRTPPSGRPFSGLNSRLKAPPSTYSGVFRTQRIDLYQQASPPDALRWMPKPWERAGPPSREGPPRRAEEPGSRGEKEPGLPPPR

Target ORF information:

RefSeq Version	XM_006524620.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524620.3

ORF Insert Sequence:

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGGCAAGTCC TTAGAGATCC AGAAACCCGC TGTTGCCCCT 
CGACATGGCC TGCAGAGTCT CGGAAAAGTT GCCATTGCCC GGCGCATGCC ACCCCCAGCC 
AACCTTCCAA GCCTGAAAGC CGAGAACAAA GGCAATGACC CCAATGTCTC ACTAGTGCCG 
AAAGACGGAA CAGGATGGGC AAGCAAACAG GAGCAGTCCG ACCCCAAGAG TTCCGATGCC 
TCAACCGCTC AGCCGCCGGA ATCGCAGCCA CTGCCGGCTT CACAGACGCC TGCCTCCAAC 
CAGCCGAAAC GACCCCCAAC AGCCCCCGAG AACACTCCTT CGGTTCCAAG CGGGGTAAAG 
TCCTGGGCAC AAGCCAGTGT CACCCATGGA GCCCATGGAG ATGGTGGAAG GGCATCAAAC 
CTACTGTCGC GATTCTCTCG AGAGGAATTT CCGACCCTGC AGGCGGCTGG AGACCAGGAC 
AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA CAGTCGTCTG GGCCCGGACC AAGCCTCCGC 
CCCCAAAATT CTACAACTTG GAGGGACGGA GGTGGGCGTG GCCCTGATGA CCTGGAGGGC 
CCGGACTCCA AACTTCATCA TGGCCATGAT CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA 
CCCCAGTTCC CTCCCTACCG CGGGATGATG CCGCCCTTTA TGTATCCCCC ATATCTCCCA 
TTCCCTCCGC CCTATGGACC CCAGGGGCCT TATAGATACC CGACTCCTGA TGGGCCCAGC 
CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC TCAGGGCCAC CAATGCGCCT TGTAGAGCCT 
GTGGGTCGGC CTTCTATTCT TAAAGAGGAT AATCTTAAAG AGTTTGACCA ACTGGACCAG 
GAAAATGATG ATGGTTGGGC AGGGGCCCAT GAAGAGGTTG ACTATACTGA AAAGCTCAAA 
TTCAGCGATG AGGAAGATGG ACGAGACTCC GACGAGGAGG GAGCTGAGGG CCACAAGGAT 
TCCCAGTCAG CTGCTGCTGA GGAACCTGAG ACAGATGGCA AAAAGGGCAC CTCCCCTGGC 
AGCGAATTGC CCCCTCCCAA GACAGCCTGG ACAGAGAATG CTCGGCCTTC AGAGACAGAG 
CCAGCTCCTC CTACCCCAAA GCCTCCCCCA CCTCCACCTC ACCGGGGGCC AGTCGGGAAC 
TGGGGGCCCC CTGGGGACTA CCCAGATCGA GGGGGTCCTC CCTGCAAACC CCCAGCACCT 
GAGGATGAGG ATGAGGCATG GCGGCAGCGT CGGAAGCAAT CTTCTTCAGA GATCTCCCTG 
GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA GAGGAAGAGC GACGCATGCA GGAGGAGCGC 
CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT CTTGATGAAA AGTTTGGGGC ACCTGACAAG 
CGGCTCAAGG CAGAGCCTGC AGCCCCACCT GTTACCCCTG CTGCCCCAGC TCTACCACCT 
GTAGTCCCCA AAGAAATTCC TGCAGCTCCA GCTCTGCCAC CAACACCTAC CCCAACACCA 
GAGAAAGAAC CTGAAGAGCC GGCACAAGCC CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG 
GTGGCTCCAG TTCCCACTCT GGTGAGTGGG GGTGGTTGCA CTGCCAACAG CAACAGCAGT 
GGCAGCTTCG AAGCCAGCCC AGTGGAACCC CAACTGCCTT CAAAAGAGGG TCCTGAACCA 
CCAGAAGAGG TTCCTCCACC TACCACACCC CCAGCCCCAA AGATGGAACC CAAAGGTGAT 
GGGGTTGGTT CTACTCGCCA GCCCCCCAGT CAGGGCTTGG GCTACCCCAA ATACCAGAAG 
TCATTGCCTC CTCGCTTCCA GCGGCAGCAA CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG 
CAACAGCAGT GGCAGCAGCA GCAACAAGGC ACTGCCCCTC CTGCCCCAGT GCCCCCATCA 
CCACCACAAC CTGTGACCCT GGGAGCTGTA CCTGCCCCAC AAGCACCGCC CCCACCTCCC 
AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG CCCCCACCAA TGCCTCCAAT GAACTTTGAC 
CCCCGCTGGA TGATGATTCC TCCTTACGTG GACCCTCGGC TTCTCCAGGG CCGGCCCCCT 
CTGGACTTCT ACCCTCCTGG TGTGCATCCC TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC 
AGTGGGGGCT CAAGCTCAGA GCCATTTGAA CGCCATGCAC CCCCTCTGTT GCGGGAAAGG 
GGCACCCCAC CAGTGGACCC CAAGTTGGCC TGGGTTGGAG ATGTCTTTAC CACCACACCC 
ACTGATCCTC GTCCTCTTAC CTCACCACTG CGCCAGGCAG CAGATGAGGA GGAGAAGAGT 
ATGAGGAGCG AGACTCCTCC GGTACCCCCG CCCCCACCTT ATCTGGCTAA TTATCCAGGT 
TTTCCAGAGA ATGGAACTCC TGGGCCCCCA ATCTCGAGAT TCCCTCTGGA GGAATCTGCT 
CCCCCGGGAC CCCGCCCACT ACCCTGGCCT CCAGGCAATG ATGAAGCTGC CAAGATGCAG 
GCTCCACCGC CCAAGAAGGA ACCCTCGAAG GAGGAACCGC CACAGCTGTC CGGGCCAGAA 
GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA GGCCCACCAC CACCACGGAG AGAGAATCGT 
ACTGAGACTC GCTGGGGCCC CCGACCAGGC AGTTGTCGCC GTGGAATCCC TCCAGAGGAG 
CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT ATAAAGAAGC CACCACCACC TGTGAAAGTA 
GAGGAGCTGC CTCCTAAGTC CCTAGAACAG GGGGATGAGA CCCCCAAGGT CCCAAAGCCA 
GATGCACTCA AGACAGCTAA GGGCAAGGTG GGCCCCAAAG AAACCCCACC AGGTGGAAAC 
CTGTCTCCTG CACCTAGGCT TCGGCGGGAC TATTCCTATG AAAGAGTAGG TCCTACATCT 
TGCCGTGGTC GGGGCAGAGG CGAGTATTTT GCCAGAGGAA GGGGTTTTCG GGGGACCTAT 
GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT GAATTCCGAA GTTACCGAGA GTTCCGAGGA 
GACGATGGGC GTGGGGGTGG TTCAGGCGGA ACAAACCATC CTTCTGCTCC CCGAGGCCGC 
ACTGCCAGTG AGACACGAAG TGAGGGTTCA GAATATGAAG AAATCCCCAA GAGACGCCGC 
CAGCGTGGCT CTGAAACAGG CAGTGAGACT CATGAGAGTG ACCTGGCCCC CTCTGACAAG 
GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT GGTCAGGTCC CTCTTGCCCC CCCACAACCA 
GGAGCTCCTC CATCACCAGC CCCAGCCCGA TTCTCCACTG CCCGAGGTGG TCGTGTCTTC 
ACTCCAAGAG GAGTACCATC ACGCCGTGGC CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC 
TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG GTTCCAAAGA AACCTCCAAC AGGTCCTTTG 
CCTCCAAGTA AAGAACCTTT GAAAGAGAAG CTGATCTCAG GGCCTCTGTC CCCCATGTCT 
CGAGCTGGCA ACATGGGTGT AGGCATGGAA GATGGGGAGC GACCCCGACG GAGGCGACAT 
GGCAGGGCTC AACAGCAGGA CAAACCACCT CGTTTCCGAA GGCTGAAACA AGAACGTGAA 
AACGCTGCCA GAGGAGCTGA TGGCAAGCCC CCTTCCCTAA CCCTTGCAGC CTCTACCCCT 
GGACCTGAGG AGACACTAAC AGCAGCCACA GTTCCCCCAC CCCCTCGCCG CACAGCTGCT 
AAGTCTCCTG ATCTGTCAAA CCAGAACTCA GACCAAGCCA ATGAGGAATG GGAGACTGCA 
TCAGAGAGCA GCGACTTTGC CAGTGAGCGC CGGGGTGACA AGGAGACTCC CCCAGCAGCA 
CTTATGACCT CTAAAGCTGT GGGGACTCCT GGGGCCAATG CAGGTGGGGC TGGACCAGGC 
ATTTCAGCCA TGTCTCGTGG AGACCTGAGC CAACGAGCCA AGGATTTGAG CAAGCGGAGC 
TTCTCAAGTC AGCGGCCAGG CATGGATCGT CAAAACCGGC GCCCAGGCAC TGGGGGCAAG 
ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT GGTGGTGCAG GCCCTGGGGG ACGGACCGGC 
CCAGGACGAG GGGATAAGAG GAGCTGGCCC TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG 
GAGCGACCTC CGGGACTTCC TCTGCCTCCG CCACCCCCCA GCAGTTCTGC TGTCTTCCGC 
CTGGACCAAG TTATCCATAG CAACCCTGCT GGAATCCAAC AGGCTCTGGC CCAACTTAGC 
AGCCGCCAAG GAAATGTAAC TGCACCAGGG GGGCACCCAA GGCCTAAGCC TGGGCCTCCC 
CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC CCAACCCGTT ATGACCCCCC AAGGGCCAGC 
AGCGCTATCA GTTCTGACCC CCACTTCGAG GAGCCAGGGC CGATGGTGAG GGGGGTGGGC 
GGGACTCCCC GGGACTCTGC CGGGGTTAAT CCCTTCCCCC CGAAACGGCG GGAGCGGCCT 
CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA ACTGTGCCAG CTTCTCATAG CTCTGGATTC 
TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT CAGGAAGAAT CCAGAGATTC AGGCACAGAG 
GCCCTAACTC CCCACATCTG GAATCGTTTA CATACTGCCA CAAGCCGAAA GAGTTACCAG 
CCTGGCTCCA TCGAGCCCTG GATGGAGCCC CTGAGTCCCT TTGAGGATGT GGCAGGCACA 
GAGATGAGTC AGTCTGACAG TGGAGTGGAC CTGAGTGGAG ATTCTCAGGT GTCATCAGGT 
CCCTGCAGTC AGCGAAGTTC CCCTGATGGA GGACTTAAGG GGTCAGCAGA GGGACCCCCC 
AAGCGGCCTG GAGGACCCTC ACCCCTGAAT GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT 
GAACCCTCTG AACCTCCTAG GAGACGGCCA CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG 
CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT CCCATAGGCA CAGAACGATC ACAGCGCACA 
GATCGAGGCC CAGAGCCAGG CCCTCTCCGA CCAGCCCACC GACCGGGCTC TCAAGTTGAG 
TTTGGCACTA CCAACAAGGA CTCAGACTTG TGCCTAGTGG TCGGAGACAC TTTAAAAGGA 
GAAAAGGAGT TGGTGGCATC AGCCACAGAG GCCGTTCCCA TATCCAGAGA CTGGGAGCTG 
CTCCCTAGTG CTTCTACTTC AGCTGAGCCA CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT 
GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT CCGTACCCTG AGGTCTTCTA TGGCAGCCCT 
GGACCTCCTA ATTCCCAGCA GGTCTCTGGG GGTGCACCCA TCGACTCTCA GTTACATCCC 
AACAGTGGAG GCTTCCGTCC TGGGACACCC TCATTGCACC AGTACAGGTC ACAGCCTCTG 
TATCTGCCCC CAGGTCCAGC CCCTCCCTCG GCACTGCTTT CTGGGGTAGC TCTCAAGGGC 
CAGTTTCTGG ATTTCTCAGC ACTGCAAGCC ACAGAGCTGG GGAAGTTGCC TGCTGGGGGA 
GTTCTCTACC CTCCGCCATC CTTCCTCTAC TCTGCAGCTT TCTGCCCCAG CCCTTTGCCT 
GACCCACCCT TGCTTCAGGT ACGCCAGGAT CTGCCATCCC CTTCAGATTT TTACTCTACT 
CCTCTGCAGC CTGGTGGCCA GAGTGGCTTC CTTCCTTCGG GAGCCCCTGC TCAGCAGATG 
CTTCTACCTG TGGTAGACTC ACAGCTGCCA GTGGTGAACT TTGGCTCCCT GCCACCAGCG 
CCACCCCCTG CTCCACCCCC CCTTTCTCTG TTACCTGTGG GTCCTGCTCT CCAGCCCCCC 
AACCTAGCTG TGCGGCCCCC ACCTGCTCCT GCTGCTCGGG TCTTACCTTC ACCCGCTAGA 
CCCTTCGCCC CTAGCTTGGG GCGAGCAGAG CTGCACCCAG TAGAACTAAA GCCTTTCCAG 
GATTACCGAA AGCTGAGCAG CAACCTTGGG GGTCCTGGGT CGTCACGGAC TCCCCCATCT 
GGAAGGCCCT TCTCTGGTCT CAATTCCCGT CTCAAGGCCC CACCTTCCAC CTACAGTGGA 
GTATTCCGAA CTCAGCGCAT TGACCTCTAC CAGCAGGCTT CCCCACCAGA TGCCCTGCGC 
TGGATGCCGA AGCCTTGGGA ACGGGCAGGG CCACCTTCTC GAGAAGGGCC TCCCCGAAGA 
GCAGAGGAGC CTGGGTCCCG GGGGGAGAAG GAGCCTGGAT TGCCCCCACC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prrc2a ( XM_006524621.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524684.1 Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X2, mRNA.

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CloneID	OMu53832
Clone ID Related Accession (Same CDS sequence)	XM_006524621.3 , XM_030249900.1 , XM_006524621.4
Accession Version	XM_006524621.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein PRRC2A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524621.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGTTGCCCCT CGACATGGCC TGCAGAGTCT CGGAAAAGTT 
GCCATTGCCC GGCGCATGCC ACCCCCAGCC AACCTTCCAA GCCTGAAAGC CGAGAACAAA 
GGCAATGACC CCAATGTCTC ACTAGTGCCG AAAGACGGAA CAGGATGGGC AAGCAAACAG 
GAGCAGTCCG ACCCCAAGAG TTCCGATGCC TCAACCGCTC AGCCGCCGGA ATCGCAGCCA 
CTGCCGGCTT CACAGACGCC TGCCTCCAAC CAGCCGAAAC GACCCCCAAC AGCCCCCGAG 
AACACTCCTT CGGTTCCAAG CGGGGTAAAG TCCTGGGCAC AAGCCAGTGT CACCCATGGA 
GCCCATGGAG ATGGTGGAAG GGCATCAAAC CTACTGTCGC GATTCTCTCG AGAGGAATTT 
CCGACCCTGC AGGCGGCTGG AGACCAGGAC AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA 
CAGTCGTCTG GGCCCGGACC AAGCCTCCGC CCCCAAAATT CTACAACTTG GAGGGACGGA 
GGTGGGCGTG GCCCTGATGA CCTGGAGGGC CCGGACTCCA AACTTCATCA TGGCCATGAT 
CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA CCCCAGTTCC CTCCCTACCG CGGGATGATG 
CCGCCCTTTA TGTATCCCCC ATATCTCCCA TTCCCTCCGC CCTATGGACC CCAGGGGCCT 
TATAGATACC CGACTCCTGA TGGGCCCAGC CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC 
TCAGGGCCAC CAATGCGCCT TGTAGAGCCT GTGGGTCGGC CTTCTATTCT TAAAGAGGAT 
AATCTTAAAG AGTTTGACCA ACTGGACCAG GAAAATGATG ATGGTTGGGC AGGGGCCCAT 
GAAGAGGTTG ACTATACTGA AAAGCTCAAA TTCAGCGATG AGGAAGATGG ACGAGACTCC 
GACGAGGAGG GAGCTGAGGG CCACAAGGAT TCCCAGTCAG CTGCTGCTGA GGAACCTGAG 
ACAGATGGCA AAAAGGGCAC CTCCCCTGGC AGCGAATTGC CCCCTCCCAA GACAGCCTGG 
ACAGAGAATG CTCGGCCTTC AGAGACAGAG CCAGCTCCTC CTACCCCAAA GCCTCCCCCA 
CCTCCACCTC ACCGGGGGCC AGTCGGGAAC TGGGGGCCCC CTGGGGACTA CCCAGATCGA 
GGGGGTCCTC CCTGCAAACC CCCAGCACCT GAGGATGAGG ATGAGGCATG GCGGCAGCGT 
CGGAAGCAAT CTTCTTCAGA GATCTCCCTG GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA 
GAGGAAGAGC GACGCATGCA GGAGGAGCGC CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT 
CTTGATGAAA AGTTTGGGGC ACCTGACAAG CGGCTCAAGG CAGAGCCTGC AGCCCCACCT 
GTTACCCCTG CTGCCCCAGC TCTACCACCT GTAGTCCCCA AAGAAATTCC TGCAGCTCCA 
GCTCTGCCAC CAACACCTAC CCCAACACCA GAGAAAGAAC CTGAAGAGCC GGCACAAGCC 
CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG GTGGCTCCAG TTCCCACTCT GGTGAGTGGG 
GGTGGTTGCA CTGCCAACAG CAACAGCAGT GGCAGCTTCG AAGCCAGCCC AGTGGAACCC 
CAACTGCCTT CAAAAGAGGG TCCTGAACCA CCAGAAGAGG TTCCTCCACC TACCACACCC 
CCAGCCCCAA AGATGGAACC CAAAGGTGAT GGGGTTGGTT CTACTCGCCA GCCCCCCAGT 
CAGGGCTTGG GCTACCCCAA ATACCAGAAG TCATTGCCTC CTCGCTTCCA GCGGCAGCAA 
CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG CAACAGCAGT GGCAGCAGCA GCAACAAGGC 
ACTGCCCCTC CTGCCCCAGT GCCCCCATCA CCACCACAAC CTGTGACCCT GGGAGCTGTA 
CCTGCCCCAC AAGCACCGCC CCCACCTCCC AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG 
CCCCCACCAA TGCCTCCAAT GAACTTTGAC CCCCGCTGGA TGATGATTCC TCCTTACGTG 
GACCCTCGGC TTCTCCAGGG CCGGCCCCCT CTGGACTTCT ACCCTCCTGG TGTGCATCCC 
TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC AGTGGGGGCT CAAGCTCAGA GCCATTTGAA 
CGCCATGCAC CCCCTCTGTT GCGGGAAAGG GGCACCCCAC CAGTGGACCC CAAGTTGGCC 
TGGGTTGGAG ATGTCTTTAC CACCACACCC ACTGATCCTC GTCCTCTTAC CTCACCACTG 
CGCCAGGCAG CAGATGAGGA GGAGAAGAGT ATGAGGAGCG AGACTCCTCC GGTACCCCCG 
CCCCCACCTT ATCTGGCTAA TTATCCAGGT TTTCCAGAGA ATGGAACTCC TGGGCCCCCA 
ATCTCGAGAT TCCCTCTGGA GGAATCTGCT CCCCCGGGAC CCCGCCCACT ACCCTGGCCT 
CCAGGCAATG ATGAAGCTGC CAAGATGCAG GCTCCACCGC CCAAGAAGGA ACCCTCGAAG 
GAGGAACCGC CACAGCTGTC CGGGCCAGAA GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA 
GGCCCACCAC CACCACGGAG AGAGAATCGT ACTGAGACTC GCTGGGGCCC CCGACCAGGC 
AGTTGTCGCC GTGGAATCCC TCCAGAGGAG CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT 
ATAAAGAAGC CACCACCACC TGTGAAAGTA GAGGAGCTGC CTCCTAAGTC CCTAGAACAG 
GGGGATGAGA CCCCCAAGGT CCCAAAGCCA GATGCACTCA AGACAGCTAA GGGCAAGGTG 
GGCCCCAAAG AAACCCCACC AGGTGGAAAC CTGTCTCCTG CACCTAGGCT TCGGCGGGAC 
TATTCCTATG AAAGAGTAGG TCCTACATCT TGCCGTGGTC GGGGCAGAGG CGAGTATTTT 
GCCAGAGGAA GGGGTTTTCG GGGGACCTAT GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT 
GAATTCCGAA GTTACCGAGA GTTCCGAGGA GACGATGGGC GTGGGGGTGG TTCAGGCGGA 
ACAAACCATC CTTCTGCTCC CCGAGGCCGC ACTGCCAGTG AGACACGAAG TGAGGGTTCA 
GAATATGAAG AAATCCCCAA GAGACGCCGC CAGCGTGGCT CTGAAACAGG CAGTGAGACT 
CATGAGAGTG ACCTGGCCCC CTCTGACAAG GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT 
GGTCAGGTCC CTCTTGCCCC CCCACAACCA GGAGCTCCTC CATCACCAGC CCCAGCCCGA 
TTCTCCACTG CCCGAGGTGG TCGTGTCTTC ACTCCAAGAG GAGTACCATC ACGCCGTGGC 
CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG 
GTTCCAAAGA AACCTCCAAC AGGTCCTTTG CCTCCAAGTA AAGAACCTTT GAAAGAGAAG 
CTGATCTCAG GGCCTCTGTC CCCCATGTCT CGAGCTGGCA ACATGGGTGT AGGCATGGAA 
GATGGGGAGC GACCCCGACG GAGGCGACAT GGCAGGGCTC AACAGCAGGA CAAACCACCT 
CGTTTCCGAA GGCTGAAACA AGAACGTGAA AACGCTGCCA GAGGAGCTGA TGGCAAGCCC 
CCTTCCCTAA CCCTTGCAGC CTCTACCCCT GGACCTGAGG AGACACTAAC AGCAGCCACA 
GTTCCCCCAC CCCCTCGCCG CACAGCTGCT AAGTCTCCTG ATCTGTCAAA CCAGAACTCA 
GACCAAGCCA ATGAGGAATG GGAGACTGCA TCAGAGAGCA GCGACTTTGC CAGTGAGCGC 
CGGGGTGACA AGGAGACTCC CCCAGCAGCA CTTATGACCT CTAAAGCTGT GGGGACTCCT 
GGGGCCAATG CAGGTGGGGC TGGACCAGGC ATTTCAGCCA TGTCTCGTGG AGACCTGAGC 
CAACGAGCCA AGGATTTGAG CAAGCGGAGC TTCTCAAGTC AGCGGCCAGG CATGGATCGT 
CAAAACCGGC GCCCAGGCAC TGGGGGCAAG ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT 
GGTGGTGCAG GCCCTGGGGG ACGGACCGGC CCAGGACGAG GGGATAAGAG GAGCTGGCCC 
TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG GAGCGACCTC CGGGACTTCC TCTGCCTCCG 
CCACCCCCCA GCAGTTCTGC TGTCTTCCGC CTGGACCAAG TTATCCATAG CAACCCTGCT 
GGAATCCAAC AGGCTCTGGC CCAACTTAGC AGCCGCCAAG GAAATGTAAC TGCACCAGGG 
GGGCACCCAA GGCCTAAGCC TGGGCCTCCC CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC 
CCAACCCGTT ATGACCCCCC AAGGGCCAGC AGCGCTATCA GTTCTGACCC CCACTTCGAG 
GAGCCAGGGC CGATGGTGAG GGGGGTGGGC GGGACTCCCC GGGACTCTGC CGGGGTTAAT 
CCCTTCCCCC CGAAACGGCG GGAGCGGCCT CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA 
ACTGTGCCAG CTTCTCATAG CTCTGGATTC TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT 
CAGGAAGAAT CCAGAGATTC AGGCACAGAG GCCCTAACTC CCCACATCTG GAATCGTTTA 
CATACTGCCA CAAGCCGAAA GAGTTACCAG CCTGGCTCCA TCGAGCCCTG GATGGAGCCC 
CTGAGTCCCT TTGAGGATGT GGCAGGCACA GAGATGAGTC AGTCTGACAG TGGAGTGGAC 
CTGAGTGGAG ATTCTCAGGT GTCATCAGGT CCCTGCAGTC AGCGAAGTTC CCCTGATGGA 
GGACTTAAGG GGTCAGCAGA GGGACCCCCC AAGCGGCCTG GAGGACCCTC ACCCCTGAAT 
GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT GAACCCTCTG AACCTCCTAG GAGACGGCCA 
CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT 
CCCATAGGCA CAGAACGATC ACAGCGCACA GATCGAGGCC CAGAGCCAGG CCCTCTCCGA 
CCAGCCCACC GACCGGGCTC TCAAGTTGAG TTTGGCACTA CCAACAAGGA CTCAGACTTG 
TGCCTAGTGG TCGGAGACAC TTTAAAAGGA GAAAAGGAGT TGGTGGCATC AGCCACAGAG 
GCCGTTCCCA TATCCAGAGA CTGGGAGCTG CTCCCTAGTG CTTCTACTTC AGCTGAGCCA 
CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT 
CCGTACCCTG AGGTCTTCTA TGGCAGCCCT GGACCTCCTA ATTCCCAGCA GGTCTCTGGG 
GGTGCACCCA TCGACTCTCA GTTACATCCC AACAGTGGAG GCTTCCGTCC TGGGACACCC 
TCATTGCACC AGTACAGGTC ACAGCCTCTG TATCTGCCCC CAGGTCCAGC CCCTCCCTCG 
GCACTGCTTT CTGGGGTAGC TCTCAAGGGC CAGTTTCTGG ATTTCTCAGC ACTGCAAGCC 
ACAGAGCTGG GGAAGTTGCC TGCTGGGGGA GTTCTCTACC CTCCGCCATC CTTCCTCTAC 
TCTGCAGCTT TCTGCCCCAG CCCTTTGCCT GACCCACCCT TGCTTCAGGT ACGCCAGGAT 
CTGCCATCCC CTTCAGATTT TTACTCTACT CCTCTGCAGC CTGGTGGCCA GAGTGGCTTC 
CTTCCTTCGG GAGCCCCTGC TCAGCAGATG CTTCTACCTG TGGTAGACTC ACAGCTGCCA 
GTGGTGAACT TTGGCTCCCT GCCACCAGCG CCACCCCCTG CTCCACCCCC CCTTTCTCTG 
TTACCTGTGG GTCCTGCTCT CCAGCCCCCC AACCTAGCTG TGCGGCCCCC ACCTGCTCCT 
GCTGCTCGGG TCTTACCTTC ACCCGCTAGA CCCTTCGCCC CTAGCTTGGG GCGAGCAGAG 
CTGCACCCAG TAGAACTAAA GCCTTTCCAG GATTACCGAA AGCTGAGCAG CAACCTTGGG 
GGTCCTGGGT CGTCACGGAC TCCCCCATCT GGAAGGCCCT TCTCTGGTCT CAATTCCCGT 
CTCAAGGCCC CACCTTCCAC CTACAGTGGA GTATTCCGAA CTCAGCGCAT TGACCTCTAC 
CAGCAGGCTT CCCCACCAGA TGCCCTGCGC TGGATGCCGA AGCCTTGGGA ACGGGCAGGG 
CCACCTTCTC GAGAAGGGCC TCCCCGAAGA GCAGAGGAGC CTGGGTCCCG GGGGGAGAAG 
GAGCCTGGAT TGCCCCCACC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524684.1
CDS	245..6691
Translation	MSDRSGPTAKGKDGKKYSSLNLFDTYKVAPRHGLQSLGKVAIARRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQSDPKSSDASTAQPPESQPLPASQTPASNQPKRPPTAPENTPSVPSGVKSWAQASVTHGAHGDGGRASNLLSRFSREEFPTLQAAGDQDKAAKERESAEQSSGPGPSLRPQNSTTWRDGGGRGPDDLEGPDSKLHHGHDPRGGLQPSGPPQFPPYRGMMPPFMYPPYLPFPPPYGPQGPYRYPTPDGPSRFPRVAGPRGSGPPMRLVEPVGRPSILKEDNLKEFDQLDQENDDGWAGAHEEVDYTEKLKFSDEEDGRDSDEEGAEGHKDSQSAAAEEPETDGKKGTSPGSELPPPKTAWTENARPSETEPAPPTPKPPPPPPHRGPVGNWGPPGDYPDRGGPPCKPPAPEDEDEAWRQRRKQSSSEISLAVERARRRREEEERRMQEERRAACAEKLKRLDEKFGAPDKRLKAEPAAPPVTPAAPALPPVVPKEIPAAPALPPTPTPTPEKEPEEPAQAPPVQAAPSPGVAPVPTLVSGGGCTANSNSSGSFEASPVEPQLPSKEGPEPPEEVPPPTTPPAPKMEPKGDGVGSTRQPPSQGLGYPKYQKSLPPRFQRQQQEQLLKQQQQQQQWQQQQQGTAPPAPVPPSPPQPVTLGAVPAPQAPPPPPKALYPGALGRPPPMPPMNFDPRWMMIPPYVDPRLLQGRPPLDFYPPGVHPSGLVPRERSDSGGSSSEPFERHAPPLLRERGTPPVDPKLAWVGDVFTTTPTDPRPLTSPLRQAADEEEKSMRSETPPVPPPPPYLANYPGFPENGTPGPPISRFPLEESAPPGPRPLPWPPGNDEAAKMQAPPPKKEPSKEEPPQLSGPEAGRKPARGGQGPPPPRRENRTETRWGPRPGSCRRGIPPEEPGVPPRRAGPIKKPPPPVKVEELPPKSLEQGDETPKVPKPDALKTAKGKVGPKETPPGGNLSPAPRLRRDYSYERVGPTSCRGRGRGEYFARGRGFRGTYGGRGRGARSREFRSYREFRGDDGRGGGSGGTNHPSAPRGRTASETRSEGSEYEEIPKRRRQRGSETGSETHESDLAPSDKEAPPPKEGVLGQVPLAPPQPGAPPSPAPARFSTARGGRVFTPRGVPSRRGRGGGRPPPVCSGWSPPAKSLVPKKPPTGPLPPSKEPLKEKLISGPLSPMSRAGNMGVGMEDGERPRRRRHGRAQQQDKPPRFRRLKQERENAARGADGKPPSLTLAASTPGPEETLTAATVPPPPRRTAAKSPDLSNQNSDQANEEWETASESSDFASERRGDKETPPAALMTSKAVGTPGANAGGAGPGISAMSRGDLSQRAKDLSKRSFSSQRPGMDRQNRRPGTGGKTGSGGGSSGGGGAGPGGRTGPGRGDKRSWPSPKNRSRPPEERPPGLPLPPPPPSSSAVFRLDQVIHSNPAGIQQALAQLSSRQGNVTAPGGHPRPKPGPPQAPQGSSPRPPTRYDPPRASSAISSDPHFEEPGPMVRGVGGTPRDSAGVNPFPPKRRERPPRKPELLQEETVPASHSSGFLGSKPEVPGPQEESRDSGTEALTPHIWNRLHTATSRKSYQPGSIEPWMEPLSPFEDVAGTEMSQSDSGVDLSGDSQVSSGPCSQRSSPDGGLKGSAEGPPKRPGGPSPLNAVPGESASGSEPSEPPRRRPPASHEGERKELPREQPLPPGPIGTERSQRTDRGPEPGPLRPAHRPGSQVEFGTTNKDSDLCLVVGDTLKGEKELVASATEAVPISRDWELLPSASTSAEPQPKSLGSGQCVPEPSPSGQRPYPEVFYGSPGPPNSQQVSGGAPIDSQLHPNSGGFRPGTPSLHQYRSQPLYLPPGPAPPSALLSGVALKGQFLDFSALQATELGKLPAGGVLYPPPSFLYSAAFCPSPLPDPPLLQVRQDLPSPSDFYSTPLQPGGQSGFLPSGAPAQQMLLPVVDSQLPVVNFGSLPPAPPPAPPPLSLLPVGPALQPPNLAVRPPPAPAARVLPSPARPFAPSLGRAELHPVELKPFQDYRKLSSNLGGPGSSRTPPSGRPFSGLNSRLKAPPSTYSGVFRTQRIDLYQQASPPDALRWMPKPWERAGPPSREGPPRRAEEPGSRGEKEPGLPPPR

Target ORF information:

RefSeq Version	XM_006524621.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus proline-rich coiled-coil 2A (Prrc2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524621.3

ORF Insert Sequence:

ATGTCCGATC GCTCGGGGCC GACTGCCAAG GGGAAGGATG GAAAGAAGTA TTCCTCGCTC 
AACCTGTTTG ATACGTATAA GGTTGCCCCT CGACATGGCC TGCAGAGTCT CGGAAAAGTT 
GCCATTGCCC GGCGCATGCC ACCCCCAGCC AACCTTCCAA GCCTGAAAGC CGAGAACAAA 
GGCAATGACC CCAATGTCTC ACTAGTGCCG AAAGACGGAA CAGGATGGGC AAGCAAACAG 
GAGCAGTCCG ACCCCAAGAG TTCCGATGCC TCAACCGCTC AGCCGCCGGA ATCGCAGCCA 
CTGCCGGCTT CACAGACGCC TGCCTCCAAC CAGCCGAAAC GACCCCCAAC AGCCCCCGAG 
AACACTCCTT CGGTTCCAAG CGGGGTAAAG TCCTGGGCAC AAGCCAGTGT CACCCATGGA 
GCCCATGGAG ATGGTGGAAG GGCATCAAAC CTACTGTCGC GATTCTCTCG AGAGGAATTT 
CCGACCCTGC AGGCGGCTGG AGACCAGGAC AAGGCTGCCA AGGAAAGGGA GTCTGCCGAA 
CAGTCGTCTG GGCCCGGACC AAGCCTCCGC CCCCAAAATT CTACAACTTG GAGGGACGGA 
GGTGGGCGTG GCCCTGATGA CCTGGAGGGC CCGGACTCCA AACTTCATCA TGGCCATGAT 
CCCCGGGGTG GGCTGCAGCC TTCAGGCCCA CCCCAGTTCC CTCCCTACCG CGGGATGATG 
CCGCCCTTTA TGTATCCCCC ATATCTCCCA TTCCCTCCGC CCTATGGACC CCAGGGGCCT 
TATAGATACC CGACTCCTGA TGGGCCCAGC CGTTTTCCCC GTGTGGCGGG CCCCCGGGGC 
TCAGGGCCAC CAATGCGCCT TGTAGAGCCT GTGGGTCGGC CTTCTATTCT TAAAGAGGAT 
AATCTTAAAG AGTTTGACCA ACTGGACCAG GAAAATGATG ATGGTTGGGC AGGGGCCCAT 
GAAGAGGTTG ACTATACTGA AAAGCTCAAA TTCAGCGATG AGGAAGATGG ACGAGACTCC 
GACGAGGAGG GAGCTGAGGG CCACAAGGAT TCCCAGTCAG CTGCTGCTGA GGAACCTGAG 
ACAGATGGCA AAAAGGGCAC CTCCCCTGGC AGCGAATTGC CCCCTCCCAA GACAGCCTGG 
ACAGAGAATG CTCGGCCTTC AGAGACAGAG CCAGCTCCTC CTACCCCAAA GCCTCCCCCA 
CCTCCACCTC ACCGGGGGCC AGTCGGGAAC TGGGGGCCCC CTGGGGACTA CCCAGATCGA 
GGGGGTCCTC CCTGCAAACC CCCAGCACCT GAGGATGAGG ATGAGGCATG GCGGCAGCGT 
CGGAAGCAAT CTTCTTCAGA GATCTCCCTG GCAGTGGAAC GAGCTCGAAG GCGCAGAGAA 
GAGGAAGAGC GACGCATGCA GGAGGAGCGC CGGGCAGCCT GTGCTGAGAA ACTTAAGCGT 
CTTGATGAAA AGTTTGGGGC ACCTGACAAG CGGCTCAAGG CAGAGCCTGC AGCCCCACCT 
GTTACCCCTG CTGCCCCAGC TCTACCACCT GTAGTCCCCA AAGAAATTCC TGCAGCTCCA 
GCTCTGCCAC CAACACCTAC CCCAACACCA GAGAAAGAAC CTGAAGAGCC GGCACAAGCC 
CCTCCTGTGC AGGCCGCCCC CAGTCCAGGG GTGGCTCCAG TTCCCACTCT GGTGAGTGGG 
GGTGGTTGCA CTGCCAACAG CAACAGCAGT GGCAGCTTCG AAGCCAGCCC AGTGGAACCC 
CAACTGCCTT CAAAAGAGGG TCCTGAACCA CCAGAAGAGG TTCCTCCACC TACCACACCC 
CCAGCCCCAA AGATGGAACC CAAAGGTGAT GGGGTTGGTT CTACTCGCCA GCCCCCCAGT 
CAGGGCTTGG GCTACCCCAA ATACCAGAAG TCATTGCCTC CTCGCTTCCA GCGGCAGCAA 
CAGGAGCAGC TCCTGAAGCA GCAGCAGCAG CAACAGCAGT GGCAGCAGCA GCAACAAGGC 
ACTGCCCCTC CTGCCCCAGT GCCCCCATCA CCACCACAAC CTGTGACCCT GGGAGCTGTA 
CCTGCCCCAC AAGCACCGCC CCCACCTCCC AAAGCCCTGT ACCCAGGTGC TCTGGGCCGG 
CCCCCACCAA TGCCTCCAAT GAACTTTGAC CCCCGCTGGA TGATGATTCC TCCTTACGTG 
GACCCTCGGC TTCTCCAGGG CCGGCCCCCT CTGGACTTCT ACCCTCCTGG TGTGCATCCC 
TCTGGCCTGG TTCCCCGGGA ACGCTCAGAC AGTGGGGGCT CAAGCTCAGA GCCATTTGAA 
CGCCATGCAC CCCCTCTGTT GCGGGAAAGG GGCACCCCAC CAGTGGACCC CAAGTTGGCC 
TGGGTTGGAG ATGTCTTTAC CACCACACCC ACTGATCCTC GTCCTCTTAC CTCACCACTG 
CGCCAGGCAG CAGATGAGGA GGAGAAGAGT ATGAGGAGCG AGACTCCTCC GGTACCCCCG 
CCCCCACCTT ATCTGGCTAA TTATCCAGGT TTTCCAGAGA ATGGAACTCC TGGGCCCCCA 
ATCTCGAGAT TCCCTCTGGA GGAATCTGCT CCCCCGGGAC CCCGCCCACT ACCCTGGCCT 
CCAGGCAATG ATGAAGCTGC CAAGATGCAG GCTCCACCGC CCAAGAAGGA ACCCTCGAAG 
GAGGAACCGC CACAGCTGTC CGGGCCAGAA GCAGGCAGAA AGCCTGCCCG TGGAGGCCAA 
GGCCCACCAC CACCACGGAG AGAGAATCGT ACTGAGACTC GCTGGGGCCC CCGACCAGGC 
AGTTGTCGCC GTGGAATCCC TCCAGAGGAG CCAGGTGTCC CTCCTCGAAG AGCTGGGCCT 
ATAAAGAAGC CACCACCACC TGTGAAAGTA GAGGAGCTGC CTCCTAAGTC CCTAGAACAG 
GGGGATGAGA CCCCCAAGGT CCCAAAGCCA GATGCACTCA AGACAGCTAA GGGCAAGGTG 
GGCCCCAAAG AAACCCCACC AGGTGGAAAC CTGTCTCCTG CACCTAGGCT TCGGCGGGAC 
TATTCCTATG AAAGAGTAGG TCCTACATCT TGCCGTGGTC GGGGCAGAGG CGAGTATTTT 
GCCAGAGGAA GGGGTTTTCG GGGGACCTAT GGGGGCCGGG GTCGGGGAGC CCGGAGCCGT 
GAATTCCGAA GTTACCGAGA GTTCCGAGGA GACGATGGGC GTGGGGGTGG TTCAGGCGGA 
ACAAACCATC CTTCTGCTCC CCGAGGCCGC ACTGCCAGTG AGACACGAAG TGAGGGTTCA 
GAATATGAAG AAATCCCCAA GAGACGCCGC CAGCGTGGCT CTGAAACAGG CAGTGAGACT 
CATGAGAGTG ACCTGGCCCC CTCTGACAAG GAAGCCCCTC CGCCCAAAGA GGGAGTGCTT 
GGTCAGGTCC CTCTTGCCCC CCCACAACCA GGAGCTCCTC CATCACCAGC CCCAGCCCGA 
TTCTCCACTG CCCGAGGTGG TCGTGTCTTC ACTCCAAGAG GAGTACCATC ACGCCGTGGC 
CGAGGTGGGG GGCGGCCCCC TCCTGTTTGC TCAGGCTGGA GCCCTCCTGC CAAGTCTCTG 
GTTCCAAAGA AACCTCCAAC AGGTCCTTTG CCTCCAAGTA AAGAACCTTT GAAAGAGAAG 
CTGATCTCAG GGCCTCTGTC CCCCATGTCT CGAGCTGGCA ACATGGGTGT AGGCATGGAA 
GATGGGGAGC GACCCCGACG GAGGCGACAT GGCAGGGCTC AACAGCAGGA CAAACCACCT 
CGTTTCCGAA GGCTGAAACA AGAACGTGAA AACGCTGCCA GAGGAGCTGA TGGCAAGCCC 
CCTTCCCTAA CCCTTGCAGC CTCTACCCCT GGACCTGAGG AGACACTAAC AGCAGCCACA 
GTTCCCCCAC CCCCTCGCCG CACAGCTGCT AAGTCTCCTG ATCTGTCAAA CCAGAACTCA 
GACCAAGCCA ATGAGGAATG GGAGACTGCA TCAGAGAGCA GCGACTTTGC CAGTGAGCGC 
CGGGGTGACA AGGAGACTCC CCCAGCAGCA CTTATGACCT CTAAAGCTGT GGGGACTCCT 
GGGGCCAATG CAGGTGGGGC TGGACCAGGC ATTTCAGCCA TGTCTCGTGG AGACCTGAGC 
CAACGAGCCA AGGATTTGAG CAAGCGGAGC TTCTCAAGTC AGCGGCCAGG CATGGATCGT 
CAAAACCGGC GCCCAGGCAC TGGGGGCAAG ACTGGCAGTG GTGGTGGCAG TAGCGGAGGT 
GGTGGTGCAG GCCCTGGGGG ACGGACCGGC CCAGGACGAG GGGATAAGAG GAGCTGGCCC 
TCTCCCAAGA ACCGAAGCCG TCCTCCTGAG GAGCGACCTC CGGGACTTCC TCTGCCTCCG 
CCACCCCCCA GCAGTTCTGC TGTCTTCCGC CTGGACCAAG TTATCCATAG CAACCCTGCT 
GGAATCCAAC AGGCTCTGGC CCAACTTAGC AGCCGCCAAG GAAATGTAAC TGCACCAGGG 
GGGCACCCAA GGCCTAAGCC TGGGCCTCCC CAAGCCCCTC AGGGCTCCTC CCCTCGGCCC 
CCAACCCGTT ATGACCCCCC AAGGGCCAGC AGCGCTATCA GTTCTGACCC CCACTTCGAG 
GAGCCAGGGC CGATGGTGAG GGGGGTGGGC GGGACTCCCC GGGACTCTGC CGGGGTTAAT 
CCCTTCCCCC CGAAACGGCG GGAGCGGCCT CCCCGAAAAC CGGAGCTGCT GCAGGAGGAA 
ACTGTGCCAG CTTCTCATAG CTCTGGATTC TTGGGCTCTA AGCCTGAGGT CCCAGGCCCT 
CAGGAAGAAT CCAGAGATTC AGGCACAGAG GCCCTAACTC CCCACATCTG GAATCGTTTA 
CATACTGCCA CAAGCCGAAA GAGTTACCAG CCTGGCTCCA TCGAGCCCTG GATGGAGCCC 
CTGAGTCCCT TTGAGGATGT GGCAGGCACA GAGATGAGTC AGTCTGACAG TGGAGTGGAC 
CTGAGTGGAG ATTCTCAGGT GTCATCAGGT CCCTGCAGTC AGCGAAGTTC CCCTGATGGA 
GGACTTAAGG GGTCAGCAGA GGGACCCCCC AAGCGGCCTG GAGGACCCTC ACCCCTGAAT 
GCTGTTCCTG GTGAGAGTGC ATCTGGCTCT GAACCCTCTG AACCTCCTAG GAGACGGCCA 
CCTGCTTCCC ATGAAGGGGA AAGAAAGGAG CTGCCCCGGG AGCAGCCTCT GCCCCCTGGT 
CCCATAGGCA CAGAACGATC ACAGCGCACA GATCGAGGCC CAGAGCCAGG CCCTCTCCGA 
CCAGCCCACC GACCGGGCTC TCAAGTTGAG TTTGGCACTA CCAACAAGGA CTCAGACTTG 
TGCCTAGTGG TCGGAGACAC TTTAAAAGGA GAAAAGGAGT TGGTGGCATC AGCCACAGAG 
GCCGTTCCCA TATCCAGAGA CTGGGAGCTG CTCCCTAGTG CTTCTACTTC AGCTGAGCCA 
CAGCCCAAGA GCCTGGGTTC TGGGCAGTGT GTTCCAGAGC CCAGCCCTTC AGGTCAGCGT 
CCGTACCCTG AGGTCTTCTA TGGCAGCCCT GGACCTCCTA ATTCCCAGCA GGTCTCTGGG 
GGTGCACCCA TCGACTCTCA GTTACATCCC AACAGTGGAG GCTTCCGTCC TGGGACACCC 
TCATTGCACC AGTACAGGTC ACAGCCTCTG TATCTGCCCC CAGGTCCAGC CCCTCCCTCG 
GCACTGCTTT CTGGGGTAGC TCTCAAGGGC CAGTTTCTGG ATTTCTCAGC ACTGCAAGCC 
ACAGAGCTGG GGAAGTTGCC TGCTGGGGGA GTTCTCTACC CTCCGCCATC CTTCCTCTAC 
TCTGCAGCTT TCTGCCCCAG CCCTTTGCCT GACCCACCCT TGCTTCAGGT ACGCCAGGAT 
CTGCCATCCC CTTCAGATTT TTACTCTACT CCTCTGCAGC CTGGTGGCCA GAGTGGCTTC 
CTTCCTTCGG GAGCCCCTGC TCAGCAGATG CTTCTACCTG TGGTAGACTC ACAGCTGCCA 
GTGGTGAACT TTGGCTCCCT GCCACCAGCG CCACCCCCTG CTCCACCCCC CCTTTCTCTG 
TTACCTGTGG GTCCTGCTCT CCAGCCCCCC AACCTAGCTG TGCGGCCCCC ACCTGCTCCT 
GCTGCTCGGG TCTTACCTTC ACCCGCTAGA CCCTTCGCCC CTAGCTTGGG GCGAGCAGAG 
CTGCACCCAG TAGAACTAAA GCCTTTCCAG GATTACCGAA AGCTGAGCAG CAACCTTGGG 
GGTCCTGGGT CGTCACGGAC TCCCCCATCT GGAAGGCCCT TCTCTGGTCT CAATTCCCGT 
CTCAAGGCCC CACCTTCCAC CTACAGTGGA GTATTCCGAA CTCAGCGCAT TGACCTCTAC 
CAGCAGGCTT CCCCACCAGA TGCCCTGCGC TGGATGCCGA AGCCTTGGGA ACGGGCAGGG 
CCACCTTCTC GAGAAGGGCC TCCCCGAAGA GCAGAGGAGC CTGGGTCCCG GGGGGAGAAG 
GAGCCTGGAT TGCCCCCACC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.
Molecular & cellular proteomics : MCP6(2)283-93(2007 Feb)
Munton RP,Tweedie-Cullen R,Livingstone-Zatchej M,Weinandy F,Waidelich M,Longo D,Gehrig P,Potthast F,Rutishauser D,Gerrits B,Panse C,Schlapbach R,Mansuy IM

Comprehensive identification of phosphorylation sites in postsynaptic density preparations.
Molecular & cellular proteomics : MCP5(5)914-22(2006 May)
Trinidad JC,Specht CG,Thalhammer A,Schoepfer R,Burlingame AL

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K

Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse.
Genome research13(12)2621-36(2003 Dec)
Xie T,Rowen L,Aguado B,Ahearn ME,Madan A,Qin S,Campbell RD,Hood L

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Prrc2a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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