Vamp5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vamp5
Entrez Gene ID	53620
Full Name	vesicle-associated membrane protein 5
Synonyms	AF119384,Camp
General protein information	Preferred Names vesicle-associated membrane protein 5 Names vesicle-associated membrane protein 5 VAMP-5 myobrevin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001080742.2	NP_001074211.1	vesicle-associated membrane protein 5
NM_016872.4	NP_058568.1	vesicle-associated membrane protein 5

Pathways from BioSystems

Homologies

Homo sapiens (human)	VAMP5	NP_006625.1
Bos taurus (cattle)	VAMP5	NP_001039941.1
Mus musculus (house mouse)	Vamp5	NP_058568.1
Rattus norvegicus (Norway rat)	Vamp5	NP_446007.1
	VAMP5	XP_001084103.2
Canis lupus familiaris (dog)	VAMP5	XP_539938.2
Pan troglodytes (chimpanzee)	VAMP5	XP_525803.3

Gene Ontology
Bibliography

Related articles in PubMed

Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung.
Ikezawa M, Tajika Y, Ueno H, Murakami T, Inoue N, Yorifuji H
Developmental dynamics : an official publication of the American Association of Anatomists(2018 Jan)

The localization of VAMP5 in skeletal and cardiac muscle.
Takahashi M, Tajika Y, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology139(4)573-82(2013 Apr)

Requirement for distinct vesicle-associated membrane proteins in insulin- and AMP-activated protein kinase (AMPK)-induced translocation of GLUT4 and CD36 in cultured cardiomyocytes.
Schwenk RW, Dirkx E, Coumans WA, Bonen A, Klip A, Glatz JF, Luiken JJ
Diabetologia53(10)2209-19(2010 Oct)

The cytoplasmic domain of Vamp4 and Vamp5 is responsible for their correct subcellular targeting: the N-terminal extenSion of VAMP4 contains a dominant autonomous targeting signal for the trans-Golgi network.
Zeng Q, Tran TT, Tan HX, Hong W
The Journal of biological chemistry278(25)23046-54(2003 Jun)

A novel synaptobrevin/VAMP homologous protein (VAMP5) is increased during in vitro myogenesis and present in the plasma membrane.
Zeng Q, Subramaniam VN, Wong SH, Tang BL, Parton RG, Rea S, James DE, Hong W
Molecular biology of the cell9(9)2423-37(1998 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study found that VAMP5 was expressed in the type IIa fibers of skeletal muscle and that in cardiomyocytes, VAMP5 expression was localized to the vicinity of intercalated discs
Title: The localization of VAMP5 in skeletal and cardiac muscle.

The cytoplasmic domain of this protein is responsible for its correct subcellular targeting.
Title: The cytoplasmic domain of Vamp4 and Vamp5 is responsible for their correct subcellular targeting: the N-terminal extenSion of VAMP4 contains a dominant autonomous targeting signal for the trans-Golgi network.

The following Vamp5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vamp5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01420	NM_016872.4 Latest version!	Mus musculus vesicle-associated membrane protein 5 (Vamp5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu01420	NM_001080742.2 Latest version!	Mus musculus vesicle-associated membrane protein 5 (Vamp5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vamp5 ( NM_001080742.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074211.1 Mus musculus vesicle-associated membrane protein 5 (Vamp5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01420
Clone ID Related Accession (Same CDS sequence)	NM_001080742.2 , NM_016872.4
Accession Version	NM_001080742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Mus musculus(house mouse)
Product	vesicle-associated membrane protein 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK009266.1 and AC116115.11. On Dec 5, 2009 this sequence version replaced NM_001080742.1. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK009266.1, BY709062.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGGGA AAGAACTGAA GCAATGCCAG CAGCAGGCGG ACGAAGTGAC GGAAATCATG 
CTCAACAATT TCGACAAGGT CCTGGAGCGT CACGGCAAGC TGGCGGAGTT GGAGCAGCGT 
TCAGACCAGC TCCTGGACAT GAGTTCGGCC TTCAGCAAGA CAACCAAGAC TTTAGCCCAG 
CAGAAGCGCT GGGAAAATAT CCGGTGCCGG GTCTACTTGG GGCTAGCAGT GGCTGTTGGC 
CTGCTCATCA TCCTGATTGT GTTGCTGGTC GTCTTTCTTC CGAGTGGTGG GGACAGTAGT 
AAACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074211.1
CDS	86..394
Translation	MAGKELKQCQQQADEVTEIMLNNFDKVLERHGKLAELEQRSDQLLDMSSAFSKTTKTLAQQKRWENIRCRVYLGLAVAVGLLIILIVLLVVFLPSGGDSSKP

Target ORF information:

RefSeq Version	NM_001080742.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus vesicle-associated membrane protein 5 (Vamp5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080742.2

ORF Insert Sequence:

ATGGCAGGGA AAGAACTGAA GCAATGCCAG CAGCAGGCGG ACGAAGTGAC GGAAATCATG 
CTCAACAATT TCGACAAGGT CCTGGAGCGT CACGGCAAGC TGGCGGAGTT GGAGCAGCGT 
TCAGACCAGC TCCTGGACAT GAGTTCGGCC TTCAGCAAGA CAACCAAGAC TTTAGCCCAG 
CAGAAGCGCT GGGAAAATAT CCGGTGCCGG GTCTACTTGG GGCTAGCAGT GGCTGTTGGC 
CTGCTCATCA TCCTGATTGT GTTGCTGGTC GTCTTTCTTC CGAGTGGTGG GGACAGTAGT 
AAACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vamp5 ( NM_016872.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058568.1 Mus musculus vesicle-associated membrane protein 5 (Vamp5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu01420
Clone ID Related Accession (Same CDS sequence)	NM_001080742.2 , NM_016872.4
Accession Version	NM_016872.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Mus musculus(house mouse)
Product	vesicle-associated membrane protein 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116115.11 and BC086925.1. On Dec 5, 2009 this sequence version replaced NM_016872.3. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC086925.1, AF035643.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGGGA AAGAACTGAA GCAATGCCAG CAGCAGGCGG ACGAAGTGAC GGAAATCATG 
CTCAACAATT TCGACAAGGT CCTGGAGCGT CACGGCAAGC TGGCGGAGTT GGAGCAGCGT 
TCAGACCAGC TCCTGGACAT GAGTTCGGCC TTCAGCAAGA CAACCAAGAC TTTAGCCCAG 
CAGAAGCGCT GGGAAAATAT CCGGTGCCGG GTCTACTTGG GGCTAGCAGT GGCTGTTGGC 
CTGCTCATCA TCCTGATTGT GTTGCTGGTC GTCTTTCTTC CGAGTGGTGG GGACAGTAGT 
AAACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058568.1
CDS	86..394
Translation	MAGKELKQCQQQADEVTEIMLNNFDKVLERHGKLAELEQRSDQLLDMSSAFSKTTKTLAQQKRWENIRCRVYLGLAVAVGLLIILIVLLVVFLPSGGDSSKP

Target ORF information:

RefSeq Version	NM_016872.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus vesicle-associated membrane protein 5 (Vamp5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016872.4

ORF Insert Sequence:

ATGGCAGGGA AAGAACTGAA GCAATGCCAG CAGCAGGCGG ACGAAGTGAC GGAAATCATG 
CTCAACAATT TCGACAAGGT CCTGGAGCGT CACGGCAAGC TGGCGGAGTT GGAGCAGCGT 
TCAGACCAGC TCCTGGACAT GAGTTCGGCC TTCAGCAAGA CAACCAAGAC TTTAGCCCAG 
CAGAAGCGCT GGGAAAATAT CCGGTGCCGG GTCTACTTGG GGCTAGCAGT GGCTGTTGGC 
CTGCTCATCA TCCTGATTGT GTTGCTGGTC GTCTTTCTTC CGAGTGGTGG GGACAGTAGT 
AAACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung.
Developmental dynamics : an official publication of the American Association of Anatomists(2018 Jan)
Ikezawa M,Tajika Y,Ueno H,Murakami T,Inoue N,Yorifuji H

The localization of VAMP5 in skeletal and cardiac muscle.
Histochemistry and cell biology139(4)573-82(2013 Apr)
Takahashi M,Tajika Y,Khairani AF,Ueno H,Murakami T,Yorifuji H

Requirement for distinct vesicle-associated membrane proteins in insulin- and AMP-activated protein kinase (AMPK)-induced translocation of GLUT4 and CD36 in cultured cardiomyocytes.
Diabetologia53(10)2209-19(2010 Oct)
Schwenk RW,Dirkx E,Coumans WA,Bonen A,Klip A,Glatz JF,Luiken JJ

The cytoplasmic domain of Vamp4 and Vamp5 is responsible for their correct subcellular targeting: the N-terminal extenSion of VAMP4 contains a dominant autonomous targeting signal for the trans-Golgi network.
The Journal of biological chemistry278(25)23046-54(2003 Jun)
Zeng Q,Tran TT,Tan HX,Hong W

A novel synaptobrevin/VAMP homologous protein (VAMP5) is increased during in vitro myogenesis and present in the plasma membrane.
Molecular biology of the cell9(9)2423-37(1998 Sep)
Zeng Q,Subramaniam VN,Wong SH,Tang BL,Parton RG,Rea S,James DE,Hong W

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Vamp5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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