Map3k6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Map3k6
Entrez Gene ID	53608
Full Name	mitogen-activated protein kinase kinase kinase 6
Synonyms	Ask2,MAPKKK6,MEKK6
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 6 Names mitogen-activated protein kinase kinase kinase 6 apoptosis signal-regulating kinase 2 map3k6
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_016693.5	NP_057902.5	mitogen-activated protein kinase kinase kinase 6
XM_006539040.4	XP_006539103.1	mitogen-activated protein kinase kinase kinase 6 isoform X1
XM_006539040.3	XP_006539103.1	mitogen-activated protein kinase kinase kinase 6 isoform X1
XM_006539041.3	XP_006539104.1	mitogen-activated protein kinase kinase kinase 6 isoform X2
XM_006539041.4	XP_006539104.1	mitogen-activated protein kinase kinase kinase 6 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MAP3K6	XP_005617983.1
Mus musculus (house mouse)	Map3k6	NP_057902.5
Homo sapiens (human)	MAP3K6	NP_004663.3
Gallus gallus (chicken)	MAP3K6	XP_003642613.2
Rattus norvegicus (Norway rat)	Map3k6	NP_001101379.1
Pan troglodytes (chimpanzee)	MAP3K6	XP_513244.2
	MAP3K6	XP_001111161.1
Bos taurus (cattle)	MAP3K6	NP_001180165.1

Gene Ontology
Bibliography

Related articles in PubMed

ASK Family Kinases Are Required for Optimal NLRP3 Inflammasome Priming.
Place DE, Samir P, Karki R, Briard B, Vogel P, Kanneganti TD
The American journal of pathology(2018 Jan)

Changes in gene methylation patterns in neonatal murine hearts: Implications for the regenerative potential.
G?rnikiewicz B, Ronowicz A, Krzemi?ski M, Sachadyn P
BMC genomics17231(2016 Mar)

The ASK family kinases differentially mediate induction of type I interferon and apoptosis during the antiviral response.
Okazaki T, Higuchi M, Takeda K, Iwatsuki-Horimoto K, Kiso M, Miyagishi M, Yanai H, Kato A, Yoneyama M, Fujita T, Taniguchi T, Kawaoka Y, Ichijo H, Gotoh Y
Science signaling8(388)ra78(2015 Aug)

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Gineste C, De Winter JM, Kohl C, Witt CC, Giannesini B, Brohm K, Le Fur Y, Gretz N, Vilmen C, Pecchi E, Jubeau M, Cozzone PJ, Stienen GJ, Granzier H, Labeit S, Ottenheijm CA, Bendahan D, Gondin J
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that deletion of either MAPKKK5 ASK1 or MAPKKK6 ASK2 in mice promoted the replication of influenza A virus in the lung.
Title: The ASK family kinases differentially mediate induction of type I interferon and apoptosis during the antiviral response.

Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1.
Title: Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1.

The following Map3k6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map3k6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu34337	XM_006539041.4 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu34336	XM_006539040.4 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu10455	NM_016693.5 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu34336	XM_006539040.3	Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu34337	XM_006539041.3	Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map3k6 ( XM_006539041.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539104.1 Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu34337
Clone ID Related Accession (Same CDS sequence)	XM_006539041.3 , XM_006539041.4
Accession Version	XM_006539041.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539041.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC CATGTCCTGG GGCCGGGGTT CTAGAACGGG CGGGCAGCTG CTGGCAGGAC 
CCGCTGGCGG AGGCGCTGAG CCGGGGCCGC TCGTCTCCTG CGGTCACGGG TCGGGGCTGC 
GCGCGGAGCC GGCCTCTCAG TGTGGTCTAC GTGCTGACCC GGGAGCCGGG GCTCGGGGTG 
GAGCCCGGGT CGGGAACCGA GGCAGAGCCG CTGCCGTTGC GATGCCTGCG CGAGGCCTGC 
GCTCAGCTCC AGGGCACACG GCCACCCCCG CAGCTACGCA GCCTACCCTT TGCGACGCTG 
GCGCTAGGCG ACACCGCTGC GCTGGACTCC TTCTACAACG CGGATGTAGT GGTGCTGGAG 
GTGAGCAGCT CCCTGGCACA GCCTTCCTTG TTCTACCACC TCGGCGTGCG TGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGTTCC CAGGCAGAGC TCCCTGATCT GCAGGCCCTT 
CGTGAGGATG TTTTCCAGAA GAACTCGGAT TGTGTTGGCA GCTACACACT GATTCCTTAT 
GTGGTGACAG CCACTGGCCG GGTCTTATGT GGCGATGCAG GCCTCCTGAG GGGCATAGCC 
GATGGGCTAG TACAGGCTGG GGCGGGCACT GAAGCCCTCC TCACTCCCCT GGTGGGCCGG 
CTTGTCCGTC TGTTGGAGGC TACACCCACA GACTCTTGCG GCTATTTCCG GGAGACCATT 
CGTCAGGATA TCCGGCAGGC TCGGGAGCGA TTCAGTGGGC AGCAGCTGAG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGGCT GGACAGCGTG GAGCTGCTGA GCCCTGACAT TGTCATGAAT 
CTGCTGCTAT CGTACCGTGA TGTCCAGGAC TACTCGGCCA TCATTGAGCT GGTGGAGACG 
CTGCAGGCTT TGCCCACCTG TGATGTGGCT GAACAGCACA ATGTCTGCTT TCACTACACA 
TTTGCCCTCA ACAGGAGGAA CAGGCCTGGG GACCGGGAGA AAGCCCTGGC TGTCCTGCTG 
CCATTGGTGA AGTATGAAGG TCCCGTGGCT CCGGATCTGT ACTGCATGTG TGGTCGTATC 
TACAAGGACA TGTTCTTCAC TTCTGGCTTC CAAAATGCCG GCCACCTGGA GCAGGCCTAT 
CACTGGTACC GCAAGGCGTT TGATGTGGAG CCCAGCCTGC ACTCAGGCAT CAATGCAGCT 
GTTCTCCTCA TTGCAGCTGG GCAGCATTTC GAAGACTCTG AGGAGCTCCG GCTGATTGGC 
ATGAAGCTGG CCTGCCTGCT AGCCCGCAAA GGCTGTGTGG AGAAGATGCA GTACTACTGG 
GATGTGGGAT TCTACCTGGG AGCCCAGATT CTTGCCAACG ACCCCATCCA GGTGGTGCTG 
GCTGCAGAGC AACTGTACAA GCTTAATGCC CCCATTTGGT ACTTGGTCTC CGTGATGGAG 
ACCTTCCTAC TGTACCAGCA TTTCAGACCC ACACCAGAGC CCTCAGGAGG ACCCCCGCTG 
CGAGCTCACT TCTGGCTCCA CTTCCTGCTA CAATCTTGCC AGCCTTTCAA GATGGCTCCT 
CCTCAGGAGG ACCAGTGCCT GGTACTGGTG CTGGAGATAA ACAAGGTCCT GCTGCCTGCC 
AGGCTTGAGA TTCAGGGAAC AGACCCCGTG AGCACAGTGA CCCTAAGCTT GCTGGACCCA 
GAGACTCAGG AGGATCCTTC CAGCTGGACC TTCCCAGTCA CCTCCATCTG TGGGATCAGC 
ACCTCCAAGC TGGACCAGCG CTGCTGCTTC CTGTACGCAC TTCCTCCGGC CCAGGACGTC 
CAGCTGTGCT TTCCCAGTGT GGAGCGCTGC CAGCGGTTCT GTGGCCTAAT TCAGGTCTTG 
GTGATGAATC CGGATTCCTC CGCGCCCACT GAGGAGGCAG AAGGCGCAAG GGAGGTGCTG 
GAGATTCTCT CAGCCTCTGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539104.1
CDS	1433..3376
Translation	MAGPCPGAGVLERAGSCWQDPLAEALSRGRSSPAVTGRGCARSRPLSVVYVLTREPGLGVEPGSGTEAEPLPLRCLREACAQLQGTRPPPQLRSLPFATLALGDTAALDSFYNADVVVLEVSSSLAQPSLFYHLGVRESFSMTNNVLLCSQAELPDLQALREDVFQKNSDCVGSYTLIPYVVTATGRVLCGDAGLLRGIADGLVQAGAGTEALLTPLVGRLVRLLEATPTDSCGYFRETIRQDIRQARERFSGQQLRQELARLQRRLDSVELLSPDIVMNLLLSYRDVQDYSAIIELVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDREKALAVLLPLVKYEGPVAPDLYCMCGRIYKDMFFTSGFQNAGHLEQAYHWYRKAFDVEPSLHSGINAAVLLIAAGQHFEDSEELRLIGMKLACLLARKGCVEKMQYYWDVGFYLGAQILANDPIQVVLAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTPEPSGGPPLRAHFWLHFLLQSCQPFKMAPPQEDQCLVLVLEINKVLLPARLEIQGTDPVSTVTLSLLDPETQEDPSSWTFPVTSICGISTSKLDQRCCFLYALPPAQDVQLCFPSVERCQRFCGLIQVLVMNPDSSAPTEEAEGAREVLEILSASA

Target ORF information:

RefSeq Version	XM_006539041.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539041.3

ORF Insert Sequence:

ATGGCGGGGC CATGTCCTGG GGCCGGGGTT CTAGAACGGG CGGGCAGCTG CTGGCAGGAC 
CCGCTGGCGG AGGCGCTGAG CCGGGGCCGC TCGTCTCCTG CGGTCACGGG TCGGGGCTGC 
GCGCGGAGCC GGCCTCTCAG TGTGGTCTAC GTGCTGACCC GGGAGCCGGG GCTCGGGGTG 
GAGCCCGGGT CGGGAACCGA GGCAGAGCCG CTGCCGTTGC GATGCCTGCG CGAGGCCTGC 
GCTCAGCTCC AGGGCACACG GCCACCCCCG CAGCTACGCA GCCTACCCTT TGCGACGCTG 
GCGCTAGGCG ACACCGCTGC GCTGGACTCC TTCTACAACG CGGATGTAGT GGTGCTGGAG 
GTGAGCAGCT CCCTGGCACA GCCTTCCTTG TTCTACCACC TCGGCGTGCG TGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGTTCC CAGGCAGAGC TCCCTGATCT GCAGGCCCTT 
CGTGAGGATG TTTTCCAGAA GAACTCGGAT TGTGTTGGCA GCTACACACT GATTCCTTAT 
GTGGTGACAG CCACTGGCCG GGTCTTATGT GGCGATGCAG GCCTCCTGAG GGGCATAGCC 
GATGGGCTAG TACAGGCTGG GGCGGGCACT GAAGCCCTCC TCACTCCCCT GGTGGGCCGG 
CTTGTCCGTC TGTTGGAGGC TACACCCACA GACTCTTGCG GCTATTTCCG GGAGACCATT 
CGTCAGGATA TCCGGCAGGC TCGGGAGCGA TTCAGTGGGC AGCAGCTGAG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGGCT GGACAGCGTG GAGCTGCTGA GCCCTGACAT TGTCATGAAT 
CTGCTGCTAT CGTACCGTGA TGTCCAGGAC TACTCGGCCA TCATTGAGCT GGTGGAGACG 
CTGCAGGCTT TGCCCACCTG TGATGTGGCT GAACAGCACA ATGTCTGCTT TCACTACACA 
TTTGCCCTCA ACAGGAGGAA CAGGCCTGGG GACCGGGAGA AAGCCCTGGC TGTCCTGCTG 
CCATTGGTGA AGTATGAAGG TCCCGTGGCT CCGGATCTGT ACTGCATGTG TGGTCGTATC 
TACAAGGACA TGTTCTTCAC TTCTGGCTTC CAAAATGCCG GCCACCTGGA GCAGGCCTAT 
CACTGGTACC GCAAGGCGTT TGATGTGGAG CCCAGCCTGC ACTCAGGCAT CAATGCAGCT 
GTTCTCCTCA TTGCAGCTGG GCAGCATTTC GAAGACTCTG AGGAGCTCCG GCTGATTGGC 
ATGAAGCTGG CCTGCCTGCT AGCCCGCAAA GGCTGTGTGG AGAAGATGCA GTACTACTGG 
GATGTGGGAT TCTACCTGGG AGCCCAGATT CTTGCCAACG ACCCCATCCA GGTGGTGCTG 
GCTGCAGAGC AACTGTACAA GCTTAATGCC CCCATTTGGT ACTTGGTCTC CGTGATGGAG 
ACCTTCCTAC TGTACCAGCA TTTCAGACCC ACACCAGAGC CCTCAGGAGG ACCCCCGCTG 
CGAGCTCACT TCTGGCTCCA CTTCCTGCTA CAATCTTGCC AGCCTTTCAA GATGGCTCCT 
CCTCAGGAGG ACCAGTGCCT GGTACTGGTG CTGGAGATAA ACAAGGTCCT GCTGCCTGCC 
AGGCTTGAGA TTCAGGGAAC AGACCCCGTG AGCACAGTGA CCCTAAGCTT GCTGGACCCA 
GAGACTCAGG AGGATCCTTC CAGCTGGACC TTCCCAGTCA CCTCCATCTG TGGGATCAGC 
ACCTCCAAGC TGGACCAGCG CTGCTGCTTC CTGTACGCAC TTCCTCCGGC CCAGGACGTC 
CAGCTGTGCT TTCCCAGTGT GGAGCGCTGC CAGCGGTTCT GTGGCCTAAT TCAGGTCTTG 
GTGATGAATC CGGATTCCTC CGCGCCCACT GAGGAGGCAG AAGGCGCAAG GGAGGTGCTG 
GAGATTCTCT CAGCCTCTGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k6 ( XM_006539041.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539104.1 Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu34337
Clone ID Related Accession (Same CDS sequence)	XM_006539041.3 , XM_006539041.4
Accession Version	XM_006539041.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539041.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC CATGTCCTGG GGCCGGGGTT CTAGAACGGG CGGGCAGCTG CTGGCAGGAC 
CCGCTGGCGG AGGCGCTGAG CCGGGGCCGC TCGTCTCCTG CGGTCACGGG TCGGGGCTGC 
GCGCGGAGCC GGCCTCTCAG TGTGGTCTAC GTGCTGACCC GGGAGCCGGG GCTCGGGGTG 
GAGCCCGGGT CGGGAACCGA GGCAGAGCCG CTGCCGTTGC GATGCCTGCG CGAGGCCTGC 
GCTCAGCTCC AGGGCACACG GCCACCCCCG CAGCTACGCA GCCTACCCTT TGCGACGCTG 
GCGCTAGGCG ACACCGCTGC GCTGGACTCC TTCTACAACG CGGATGTAGT GGTGCTGGAG 
GTGAGCAGCT CCCTGGCACA GCCTTCCTTG TTCTACCACC TCGGCGTGCG TGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGTTCC CAGGCAGAGC TCCCTGATCT GCAGGCCCTT 
CGTGAGGATG TTTTCCAGAA GAACTCGGAT TGTGTTGGCA GCTACACACT GATTCCTTAT 
GTGGTGACAG CCACTGGCCG GGTCTTATGT GGCGATGCAG GCCTCCTGAG GGGCATAGCC 
GATGGGCTAG TACAGGCTGG GGCGGGCACT GAAGCCCTCC TCACTCCCCT GGTGGGCCGG 
CTTGTCCGTC TGTTGGAGGC TACACCCACA GACTCTTGCG GCTATTTCCG GGAGACCATT 
CGTCAGGATA TCCGGCAGGC TCGGGAGCGA TTCAGTGGGC AGCAGCTGAG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGGCT GGACAGCGTG GAGCTGCTGA GCCCTGACAT TGTCATGAAT 
CTGCTGCTAT CGTACCGTGA TGTCCAGGAC TACTCGGCCA TCATTGAGCT GGTGGAGACG 
CTGCAGGCTT TGCCCACCTG TGATGTGGCT GAACAGCACA ATGTCTGCTT TCACTACACA 
TTTGCCCTCA ACAGGAGGAA CAGGCCTGGG GACCGGGAGA AAGCCCTGGC TGTCCTGCTG 
CCATTGGTGA AGTATGAAGG TCCCGTGGCT CCGGATCTGT ACTGCATGTG TGGTCGTATC 
TACAAGGACA TGTTCTTCAC TTCTGGCTTC CAAAATGCCG GCCACCTGGA GCAGGCCTAT 
CACTGGTACC GCAAGGCGTT TGATGTGGAG CCCAGCCTGC ACTCAGGCAT CAATGCAGCT 
GTTCTCCTCA TTGCAGCTGG GCAGCATTTC GAAGACTCTG AGGAGCTCCG GCTGATTGGC 
ATGAAGCTGG CCTGCCTGCT AGCCCGCAAA GGCTGTGTGG AGAAGATGCA GTACTACTGG 
GATGTGGGAT TCTACCTGGG AGCCCAGATT CTTGCCAACG ACCCCATCCA GGTGGTGCTG 
GCTGCAGAGC AACTGTACAA GCTTAATGCC CCCATTTGGT ACTTGGTCTC CGTGATGGAG 
ACCTTCCTAC TGTACCAGCA TTTCAGACCC ACACCAGAGC CCTCAGGAGG ACCCCCGCTG 
CGAGCTCACT TCTGGCTCCA CTTCCTGCTA CAATCTTGCC AGCCTTTCAA GATGGCTCCT 
CCTCAGGAGG ACCAGTGCCT GGTACTGGTG CTGGAGATAA ACAAGGTCCT GCTGCCTGCC 
AGGCTTGAGA TTCAGGGAAC AGACCCCGTG AGCACAGTGA CCCTAAGCTT GCTGGACCCA 
GAGACTCAGG AGGATCCTTC CAGCTGGACC TTCCCAGTCA CCTCCATCTG TGGGATCAGC 
ACCTCCAAGC TGGACCAGCG CTGCTGCTTC CTGTACGCAC TTCCTCCGGC CCAGGACGTC 
CAGCTGTGCT TTCCCAGTGT GGAGCGCTGC CAGCGGTTCT GTGGCCTAAT TCAGGTCTTG 
GTGATGAATC CGGATTCCTC CGCGCCCACT GAGGAGGCAG AAGGCGCAAG GGAGGTGCTG 
GAGATTCTCT CAGCCTCTGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539104.1
CDS	3111..5054
Translation	MAGPCPGAGVLERAGSCWQDPLAEALSRGRSSPAVTGRGCARSRPLSVVYVLTREPGLGVEPGSGTEAEPLPLRCLREACAQLQGTRPPPQLRSLPFATLALGDTAALDSFYNADVVVLEVSSSLAQPSLFYHLGVRESFSMTNNVLLCSQAELPDLQALREDVFQKNSDCVGSYTLIPYVVTATGRVLCGDAGLLRGIADGLVQAGAGTEALLTPLVGRLVRLLEATPTDSCGYFRETIRQDIRQARERFSGQQLRQELARLQRRLDSVELLSPDIVMNLLLSYRDVQDYSAIIELVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDREKALAVLLPLVKYEGPVAPDLYCMCGRIYKDMFFTSGFQNAGHLEQAYHWYRKAFDVEPSLHSGINAAVLLIAAGQHFEDSEELRLIGMKLACLLARKGCVEKMQYYWDVGFYLGAQILANDPIQVVLAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTPEPSGGPPLRAHFWLHFLLQSCQPFKMAPPQEDQCLVLVLEINKVLLPARLEIQGTDPVSTVTLSLLDPETQEDPSSWTFPVTSICGISTSKLDQRCCFLYALPPAQDVQLCFPSVERCQRFCGLIQVLVMNPDSSAPTEEAEGAREVLEILSASA

Target ORF information:

RefSeq Version	XM_006539041.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539041.4

ORF Insert Sequence:

ATGGCGGGGC CATGTCCTGG GGCCGGGGTT CTAGAACGGG CGGGCAGCTG CTGGCAGGAC 
CCGCTGGCGG AGGCGCTGAG CCGGGGCCGC TCGTCTCCTG CGGTCACGGG TCGGGGCTGC 
GCGCGGAGCC GGCCTCTCAG TGTGGTCTAC GTGCTGACCC GGGAGCCGGG GCTCGGGGTG 
GAGCCCGGGT CGGGAACCGA GGCAGAGCCG CTGCCGTTGC GATGCCTGCG CGAGGCCTGC 
GCTCAGCTCC AGGGCACACG GCCACCCCCG CAGCTACGCA GCCTACCCTT TGCGACGCTG 
GCGCTAGGCG ACACCGCTGC GCTGGACTCC TTCTACAACG CGGATGTAGT GGTGCTGGAG 
GTGAGCAGCT CCCTGGCACA GCCTTCCTTG TTCTACCACC TCGGCGTGCG TGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGTTCC CAGGCAGAGC TCCCTGATCT GCAGGCCCTT 
CGTGAGGATG TTTTCCAGAA GAACTCGGAT TGTGTTGGCA GCTACACACT GATTCCTTAT 
GTGGTGACAG CCACTGGCCG GGTCTTATGT GGCGATGCAG GCCTCCTGAG GGGCATAGCC 
GATGGGCTAG TACAGGCTGG GGCGGGCACT GAAGCCCTCC TCACTCCCCT GGTGGGCCGG 
CTTGTCCGTC TGTTGGAGGC TACACCCACA GACTCTTGCG GCTATTTCCG GGAGACCATT 
CGTCAGGATA TCCGGCAGGC TCGGGAGCGA TTCAGTGGGC AGCAGCTGAG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGGCT GGACAGCGTG GAGCTGCTGA GCCCTGACAT TGTCATGAAT 
CTGCTGCTAT CGTACCGTGA TGTCCAGGAC TACTCGGCCA TCATTGAGCT GGTGGAGACG 
CTGCAGGCTT TGCCCACCTG TGATGTGGCT GAACAGCACA ATGTCTGCTT TCACTACACA 
TTTGCCCTCA ACAGGAGGAA CAGGCCTGGG GACCGGGAGA AAGCCCTGGC TGTCCTGCTG 
CCATTGGTGA AGTATGAAGG TCCCGTGGCT CCGGATCTGT ACTGCATGTG TGGTCGTATC 
TACAAGGACA TGTTCTTCAC TTCTGGCTTC CAAAATGCCG GCCACCTGGA GCAGGCCTAT 
CACTGGTACC GCAAGGCGTT TGATGTGGAG CCCAGCCTGC ACTCAGGCAT CAATGCAGCT 
GTTCTCCTCA TTGCAGCTGG GCAGCATTTC GAAGACTCTG AGGAGCTCCG GCTGATTGGC 
ATGAAGCTGG CCTGCCTGCT AGCCCGCAAA GGCTGTGTGG AGAAGATGCA GTACTACTGG 
GATGTGGGAT TCTACCTGGG AGCCCAGATT CTTGCCAACG ACCCCATCCA GGTGGTGCTG 
GCTGCAGAGC AACTGTACAA GCTTAATGCC CCCATTTGGT ACTTGGTCTC CGTGATGGAG 
ACCTTCCTAC TGTACCAGCA TTTCAGACCC ACACCAGAGC CCTCAGGAGG ACCCCCGCTG 
CGAGCTCACT TCTGGCTCCA CTTCCTGCTA CAATCTTGCC AGCCTTTCAA GATGGCTCCT 
CCTCAGGAGG ACCAGTGCCT GGTACTGGTG CTGGAGATAA ACAAGGTCCT GCTGCCTGCC 
AGGCTTGAGA TTCAGGGAAC AGACCCCGTG AGCACAGTGA CCCTAAGCTT GCTGGACCCA 
GAGACTCAGG AGGATCCTTC CAGCTGGACC TTCCCAGTCA CCTCCATCTG TGGGATCAGC 
ACCTCCAAGC TGGACCAGCG CTGCTGCTTC CTGTACGCAC TTCCTCCGGC CCAGGACGTC 
CAGCTGTGCT TTCCCAGTGT GGAGCGCTGC CAGCGGTTCT GTGGCCTAAT TCAGGTCTTG 
GTGATGAATC CGGATTCCTC CGCGCCCACT GAGGAGGCAG AAGGCGCAAG GGAGGTGCTG 
GAGATTCTCT CAGCCTCTGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k6 ( XM_006539040.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539103.1 Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34336
Clone ID Related Accession (Same CDS sequence)	XM_006539040.4 , XM_006539040.3
Accession Version	XM_006539040.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539040.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC CATGTCCTGG GGCCGGGGTT CTAGAACGGG CGGGCAGCTG CTGGCAGGAC 
CCGCTGGCGG AGGCGCTGAG CCGGGGCCGC TCGTCTCCTG CGGTCACGGG TCGGGGCTGC 
GCGCGGAGCC GGCCTCTCAG TGTGGTCTAC GTGCTGACCC GGGAGCCGGG GCTCGGGGTG 
GAGCCCGGGT CGGGAACCGA GGCAGAGCCG CTGCCGTTGC GATGCCTGCG CGAGGCCTGC 
GCTCAGCTCC AGGGCACACG GCCACCCCCG CAGCTACGCA GCCTACCCTT TGCGACGCTG 
GCGCTAGGCG ACACCGCTGC GCTGGACTCC TTCTACAACG CGGATGTAGT GGTGCTGGAG 
GTGAGCAGCT CCCTGGCACA GCCTTCCTTG TTCTACCACC TCGGCGTGCG TGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGTTCC CAGGCAGAGC TCCCTGATCT GCAGGCCCTT 
CGTGATTGTG TTGGCAGCTA CACACTGATT CCTTATGTGG TGACAGCCAC TGGCCGGGTC 
TTATGTGGCG ATGCAGGCCT CCTGAGGGGC ATAGCCGATG GGCTAGTACA GGCTGGGGCG 
GGCACTGAAG CCCTCCTCAC TCCCCTGGTG GGCCGGCTTG TCCGTCTGTT GGAGGCTACA 
CCCACAGACT CTTGCGGCTA TTTCCGGGAG ACCATTCGTC AGGATATCCG GCAGGCTCGG 
GAGCGATTCA GTGGGCAGCA GCTGAGGCAG GAGCTGGCTC GCCTGCAGCG GAGGCTGGAC 
AGCGTGGAGC TGCTGAGCCC TGACATTGTC ATGAATCTGC TGCTATCGTA CCGTGATGTC 
CAGGACTACT CGGCCATCAT TGAGCTGGTG GAGACGCTGC AGGCTTTGCC CACCTGTGAT 
GTGGCTGAAC AGCACAATGT CTGCTTTCAC TACACATTTG CCCTCAACAG GAGGAACAGG 
CCTGGGGACC GGGAGAAAGC CCTGGCTGTC CTGCTGCCAT TGGTGAAGTA TGAAGGTCCC 
GTGGCTCCGG ATCTGTACTG CATGTGTGGT CGTATCTACA AGGACATGTT CTTCACTTCT 
GGCTTCCAAA ATGCCGGCCA CCTGGAGCAG GCCTATCACT GGTACCGCAA GGCGTTTGAT 
GTGGAGCCCA GCCTGCACTC AGGCATCAAT GCAGCTGTTC TCCTCATTGC AGCTGGGCAG 
CATTTCGAAG ACTCTGAGGA GCTCCGGCTG ATTGGCATGA AGCTGGCCTG CCTGCTAGCC 
CGCAAAGGCT GTGTGGAGAA GATGCAGTAC TACTGGGATG TGGGATTCTA CCTGGGAGCC 
CAGATTCTTG CCAACGACCC CATCCAGGTG GTGCTGGCTG CAGAGCAACT GTACAAGCTT 
AATGCCCCCA TTTGGTACTT GGTCTCCGTG ATGGAGACCT TCCTACTGTA CCAGCATTTC 
AGACCCACAC CAGAGCCCTC AGGAGGACCC CCGCTGCGAG CTCACTTCTG GCTCCACTTC 
CTGCTACAAT CTTGCCAGCC TTTCAAGATG GCTCCTCCTC AGGAGGACCA GTGCCTGGTA 
CTGGTGCTGG AGATAAACAA GGTCCTGCTG CCTGCCAGGC TTGAGATTCA GGGAACAGAC 
CCCGTGAGCA CAGTGACCCT AAGCTTGCTG GACCCAGAGA CTCAGGAGGA TCCTTCCAGC 
TGGACCTTCC CAGTCACCTC CATCTGTGGG ATCAGCACCT CCAAGCTGGA CCAGCGCTGC 
TGCTTCCTGT ACGCACTTCC TCCGGCCCAG GACGTCCAGC TGTGCTTTCC CAGTGTGGAG 
CGCTGCCAGC GGTTCTGTGG CCTAATTCAG GTCTTGGTGA TGAATCCGGA TTCCTCCGCG 
CCCACTGAGG AGGCAGAAGG CGCAAGGGAG GTGCTGGAGT TTGATTATGA ATACTCGGAG 
ACTGGTGAGC GGCTGGTGTT GGGCAGAGGC ACGTATGGGG TGGTGTATGC TGGCCGAGAC 
AGGCACACGA GGGTACGAAT CGCCATCAAG GAGATCCCAG AGAGAGATAG CAGATTCTCT 
CAGCCTCTGC ATGAAGAGAT CGCTCTTCAC AAACGACTTC GTCATAAGAA TATTGTGCGT 
TATCTGGGCT CAGCCAGCCA GGGCGGCTAC CTCAAGATCT TCATGGAGGA AGTGCCTGGA 
GGCAGCCTGT CCTCCTTGCT TAGGTCAGTG TGGGGACCCC TAAAGGACAA CGAGAGCACT 
ATTAGCTTCT ATACACGTCA GATCCTACAA GGACTCAGCT ACCTCCATGA GAACCGTATC 
GTTCACAGAG ACATCAAGGG AGACAATGTT CTGATCAACA CCTTCAGTGG GCTGCTCAAG 
ATTTCTGACT TTGGCACCTC TAAGCGGCTG GCAGGCATCA CACCGTGCAC GGAGACTTTC 
ACAGGGACTC TGCAGTATAT GGCCCCGGAA ATCATCGACC AGGGCCCGCG AGGATATGGG 
AAGGCAGCTG ACATCTGGTC TCTGGGCTGC ACTGTAATCG AGATGGCAAC AGGTCGACCG 
CCCTTCCATG AACTAGGGAG CCCTCAGGCT GCGATGTTTC AGGTGGGCAT GTACAAGGTA 
CATCCACCCG TGCCCGGTTC CCTGTCAGCT GAGGCCCAAG CCTTCCTCCT CCGAACTTTT 
GAGCCAGATC CCCGCCTCCG AGCCAGTGCC CAAGAGCTGC TGGGAGACCC CTTCCTTCAG 
CCAGGAAAGA GGAGCCGCAG CCCGGGCTCT CCTCGGCATA CTCCCCGGCC CTCAGGCGCC 
CCTTCCGGTC CTTCCAGTCC TTCAGCTGAC TCAGCCACAC AGTCTCAGAC ATTCCCAAGG 
CCCCAGGCAC CCTCTCAGCA CCCACCCAGC CCCCCAAAGC GCTGCCTTAG TTATGGGGAC 
ACCAGTCAGC TCCGTGTGCC CGAGGAGCCC GCTGCCGAGG AACCCGCGTC CCCAGAAGAG 
AGTTCAGGCC TGAGTCTGCT GCACCAGGAG AGCAAGCGCC GGGCCATGCT GGCTGCGGTG 
CTGGAACAGG AGGTGCCCAC ACTAGCAGAG AATCTCCTGG AACAGGAACA GGACTCTCGA 
CTCAGCAAGA TTCATGTGGA ACTTCTGCTT CGGTGCCTTG GGGCACAAAT CCACACTCCT 
AACCGCCGGC AGCTGGCCCA GGAGCTGCGG ACCCTGCAAG CTCAGCTGCG GGCCCAGAGC 
CTGGGCCCTG CGCTTTTGAA GGGGCCGCTC TTCGCCTTTC CAGACGCGGT GAAGCAGATC 
CTCCGCAGAC GCCAGATCCG CCCACACTGG ATGTTCGTGT TGGACTCGCT GCTCAGCCGT 
GCAGTCCGGG CGGCGATGGC GGTGCTGGAC GCGGAGTCGG AGAAGAAAGC GGTCTTACCG 
AGGTCAGAAG AGTCGAGTAA AGAGGAGTCC CAGCAGAAAC CTCAGGAGAG CCAGGCGCTG 
CAGAGCCAGC TCCCACCAGA GCAGGGACCC CCATCGTTGA TGGTGGAGTT GGGCCTTTTG 
CGAGCCGAGA CTGACAGGCT TCGGGACCTT CTGGCTGAGA AGGAACGTGA GTGCCAGGCG 
CTGGTGCAAC AGGCCCTGCA TCGGGTGCAT GCAGAGACCA GGAAGTATGC CCCAGCTTCA 
GAGACCCCAG CCACTCTCCC AAAGGACCAG AACCTGGTGC GGTGGCTACA GGAACTGAGT 
GTAGATCCAG CCACTATCCA AACGCTCCTG AGTCATAGCT TCACCCTTCA AACCCTGCTC 
ACCTGTGCCA CTCAAGATGA TCTAGTGTAC ACCAGAATCA GGGGAGGGAT GGTATGCCGG 
ATTTGGAGAG CCATCTTGGC ACAGCGAGCA GGAGCCACAT CGGTCACCCC AGTACCCCGG 
GACGCTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539103.1
CDS	3111..6962
Translation	MAGPCPGAGVLERAGSCWQDPLAEALSRGRSSPAVTGRGCARSRPLSVVYVLTREPGLGVEPGSGTEAEPLPLRCLREACAQLQGTRPPPQLRSLPFATLALGDTAALDSFYNADVVVLEVSSSLAQPSLFYHLGVRESFSMTNNVLLCSQAELPDLQALRDCVGSYTLIPYVVTATGRVLCGDAGLLRGIADGLVQAGAGTEALLTPLVGRLVRLLEATPTDSCGYFRETIRQDIRQARERFSGQQLRQELARLQRRLDSVELLSPDIVMNLLLSYRDVQDYSAIIELVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDREKALAVLLPLVKYEGPVAPDLYCMCGRIYKDMFFTSGFQNAGHLEQAYHWYRKAFDVEPSLHSGINAAVLLIAAGQHFEDSEELRLIGMKLACLLARKGCVEKMQYYWDVGFYLGAQILANDPIQVVLAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTPEPSGGPPLRAHFWLHFLLQSCQPFKMAPPQEDQCLVLVLEINKVLLPARLEIQGTDPVSTVTLSLLDPETQEDPSSWTFPVTSICGISTSKLDQRCCFLYALPPAQDVQLCFPSVERCQRFCGLIQVLVMNPDSSAPTEEAEGAREVLEFDYEYSETGERLVLGRGTYGVVYAGRDRHTRVRIAIKEIPERDSRFSQPLHEEIALHKRLRHKNIVRYLGSASQGGYLKIFMEEVPGGSLSSLLRSVWGPLKDNESTISFYTRQILQGLSYLHENRIVHRDIKGDNVLINTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGRPPFHELGSPQAAMFQVGMYKVHPPVPGSLSAEAQAFLLRTFEPDPRLRASAQELLGDPFLQPGKRSRSPGSPRHTPRPSGAPSGPSSPSADSATQSQTFPRPQAPSQHPPSPPKRCLSYGDTSQLRVPEEPAAEEPASPEESSGLSLLHQESKRRAMLAAVLEQEVPTLAENLLEQEQDSRLSKIHVELLLRCLGAQIHTPNRRQLAQELRTLQAQLRAQSLGPALLKGPLFAFPDAVKQILRRRQIRPHWMFVLDSLLSRAVRAAMAVLDAESEKKAVLPRSEESSKEESQQKPQESQALQSQLPPEQGPPSLMVELGLLRAETDRLRDLLAEKERECQALVQQALHRVHAETRKYAPASETPATLPKDQNLVRWLQELSVDPATIQTLLSHSFTLQTLLTCATQDDLVYTRIRGGMVCRIWRAILAQRAGATSVTPVPRDAE

Target ORF information:

RefSeq Version	XM_006539040.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539040.4

ORF Insert Sequence:

ATGGCGGGGC CATGTCCTGG GGCCGGGGTT CTAGAACGGG CGGGCAGCTG CTGGCAGGAC 
CCGCTGGCGG AGGCGCTGAG CCGGGGCCGC TCGTCTCCTG CGGTCACGGG TCGGGGCTGC 
GCGCGGAGCC GGCCTCTCAG TGTGGTCTAC GTGCTGACCC GGGAGCCGGG GCTCGGGGTG 
GAGCCCGGGT CGGGAACCGA GGCAGAGCCG CTGCCGTTGC GATGCCTGCG CGAGGCCTGC 
GCTCAGCTCC AGGGCACACG GCCACCCCCG CAGCTACGCA GCCTACCCTT TGCGACGCTG 
GCGCTAGGCG ACACCGCTGC GCTGGACTCC TTCTACAACG CGGATGTAGT GGTGCTGGAG 
GTGAGCAGCT CCCTGGCACA GCCTTCCTTG TTCTACCACC TCGGCGTGCG TGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGTTCC CAGGCAGAGC TCCCTGATCT GCAGGCCCTT 
CGTGATTGTG TTGGCAGCTA CACACTGATT CCTTATGTGG TGACAGCCAC TGGCCGGGTC 
TTATGTGGCG ATGCAGGCCT CCTGAGGGGC ATAGCCGATG GGCTAGTACA GGCTGGGGCG 
GGCACTGAAG CCCTCCTCAC TCCCCTGGTG GGCCGGCTTG TCCGTCTGTT GGAGGCTACA 
CCCACAGACT CTTGCGGCTA TTTCCGGGAG ACCATTCGTC AGGATATCCG GCAGGCTCGG 
GAGCGATTCA GTGGGCAGCA GCTGAGGCAG GAGCTGGCTC GCCTGCAGCG GAGGCTGGAC 
AGCGTGGAGC TGCTGAGCCC TGACATTGTC ATGAATCTGC TGCTATCGTA CCGTGATGTC 
CAGGACTACT CGGCCATCAT TGAGCTGGTG GAGACGCTGC AGGCTTTGCC CACCTGTGAT 
GTGGCTGAAC AGCACAATGT CTGCTTTCAC TACACATTTG CCCTCAACAG GAGGAACAGG 
CCTGGGGACC GGGAGAAAGC CCTGGCTGTC CTGCTGCCAT TGGTGAAGTA TGAAGGTCCC 
GTGGCTCCGG ATCTGTACTG CATGTGTGGT CGTATCTACA AGGACATGTT CTTCACTTCT 
GGCTTCCAAA ATGCCGGCCA CCTGGAGCAG GCCTATCACT GGTACCGCAA GGCGTTTGAT 
GTGGAGCCCA GCCTGCACTC AGGCATCAAT GCAGCTGTTC TCCTCATTGC AGCTGGGCAG 
CATTTCGAAG ACTCTGAGGA GCTCCGGCTG ATTGGCATGA AGCTGGCCTG CCTGCTAGCC 
CGCAAAGGCT GTGTGGAGAA GATGCAGTAC TACTGGGATG TGGGATTCTA CCTGGGAGCC 
CAGATTCTTG CCAACGACCC CATCCAGGTG GTGCTGGCTG CAGAGCAACT GTACAAGCTT 
AATGCCCCCA TTTGGTACTT GGTCTCCGTG ATGGAGACCT TCCTACTGTA CCAGCATTTC 
AGACCCACAC CAGAGCCCTC AGGAGGACCC CCGCTGCGAG CTCACTTCTG GCTCCACTTC 
CTGCTACAAT CTTGCCAGCC TTTCAAGATG GCTCCTCCTC AGGAGGACCA GTGCCTGGTA 
CTGGTGCTGG AGATAAACAA GGTCCTGCTG CCTGCCAGGC TTGAGATTCA GGGAACAGAC 
CCCGTGAGCA CAGTGACCCT AAGCTTGCTG GACCCAGAGA CTCAGGAGGA TCCTTCCAGC 
TGGACCTTCC CAGTCACCTC CATCTGTGGG ATCAGCACCT CCAAGCTGGA CCAGCGCTGC 
TGCTTCCTGT ACGCACTTCC TCCGGCCCAG GACGTCCAGC TGTGCTTTCC CAGTGTGGAG 
CGCTGCCAGC GGTTCTGTGG CCTAATTCAG GTCTTGGTGA TGAATCCGGA TTCCTCCGCG 
CCCACTGAGG AGGCAGAAGG CGCAAGGGAG GTGCTGGAGT TTGATTATGA ATACTCGGAG 
ACTGGTGAGC GGCTGGTGTT GGGCAGAGGC ACGTATGGGG TGGTGTATGC TGGCCGAGAC 
AGGCACACGA GGGTACGAAT CGCCATCAAG GAGATCCCAG AGAGAGATAG CAGATTCTCT 
CAGCCTCTGC ATGAAGAGAT CGCTCTTCAC AAACGACTTC GTCATAAGAA TATTGTGCGT 
TATCTGGGCT CAGCCAGCCA GGGCGGCTAC CTCAAGATCT TCATGGAGGA AGTGCCTGGA 
GGCAGCCTGT CCTCCTTGCT TAGGTCAGTG TGGGGACCCC TAAAGGACAA CGAGAGCACT 
ATTAGCTTCT ATACACGTCA GATCCTACAA GGACTCAGCT ACCTCCATGA GAACCGTATC 
GTTCACAGAG ACATCAAGGG AGACAATGTT CTGATCAACA CCTTCAGTGG GCTGCTCAAG 
ATTTCTGACT TTGGCACCTC TAAGCGGCTG GCAGGCATCA CACCGTGCAC GGAGACTTTC 
ACAGGGACTC TGCAGTATAT GGCCCCGGAA ATCATCGACC AGGGCCCGCG AGGATATGGG 
AAGGCAGCTG ACATCTGGTC TCTGGGCTGC ACTGTAATCG AGATGGCAAC AGGTCGACCG 
CCCTTCCATG AACTAGGGAG CCCTCAGGCT GCGATGTTTC AGGTGGGCAT GTACAAGGTA 
CATCCACCCG TGCCCGGTTC CCTGTCAGCT GAGGCCCAAG CCTTCCTCCT CCGAACTTTT 
GAGCCAGATC CCCGCCTCCG AGCCAGTGCC CAAGAGCTGC TGGGAGACCC CTTCCTTCAG 
CCAGGAAAGA GGAGCCGCAG CCCGGGCTCT CCTCGGCATA CTCCCCGGCC CTCAGGCGCC 
CCTTCCGGTC CTTCCAGTCC TTCAGCTGAC TCAGCCACAC AGTCTCAGAC ATTCCCAAGG 
CCCCAGGCAC CCTCTCAGCA CCCACCCAGC CCCCCAAAGC GCTGCCTTAG TTATGGGGAC 
ACCAGTCAGC TCCGTGTGCC CGAGGAGCCC GCTGCCGAGG AACCCGCGTC CCCAGAAGAG 
AGTTCAGGCC TGAGTCTGCT GCACCAGGAG AGCAAGCGCC GGGCCATGCT GGCTGCGGTG 
CTGGAACAGG AGGTGCCCAC ACTAGCAGAG AATCTCCTGG AACAGGAACA GGACTCTCGA 
CTCAGCAAGA TTCATGTGGA ACTTCTGCTT CGGTGCCTTG GGGCACAAAT CCACACTCCT 
AACCGCCGGC AGCTGGCCCA GGAGCTGCGG ACCCTGCAAG CTCAGCTGCG GGCCCAGAGC 
CTGGGCCCTG CGCTTTTGAA GGGGCCGCTC TTCGCCTTTC CAGACGCGGT GAAGCAGATC 
CTCCGCAGAC GCCAGATCCG CCCACACTGG ATGTTCGTGT TGGACTCGCT GCTCAGCCGT 
GCAGTCCGGG CGGCGATGGC GGTGCTGGAC GCGGAGTCGG AGAAGAAAGC GGTCTTACCG 
AGGTCAGAAG AGTCGAGTAA AGAGGAGTCC CAGCAGAAAC CTCAGGAGAG CCAGGCGCTG 
CAGAGCCAGC TCCCACCAGA GCAGGGACCC CCATCGTTGA TGGTGGAGTT GGGCCTTTTG 
CGAGCCGAGA CTGACAGGCT TCGGGACCTT CTGGCTGAGA AGGAACGTGA GTGCCAGGCG 
CTGGTGCAAC AGGCCCTGCA TCGGGTGCAT GCAGAGACCA GGAAGTATGC CCCAGCTTCA 
GAGACCCCAG CCACTCTCCC AAAGGACCAG AACCTGGTGC GGTGGCTACA GGAACTGAGT 
GTAGATCCAG CCACTATCCA AACGCTCCTG AGTCATAGCT TCACCCTTCA AACCCTGCTC 
ACCTGTGCCA CTCAAGATGA TCTAGTGTAC ACCAGAATCA GGGGAGGGAT GGTATGCCGG 
ATTTGGAGAG CCATCTTGGC ACAGCGAGCA GGAGCCACAT CGGTCACCCC AGTACCCCGG 
GACGCTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k6 ( NM_016693.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_057902.5 Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), mRNA.

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CloneID	OMu10455
Clone ID Related Accession (Same CDS sequence)	NM_016693.5
Accession Version	NM_016693.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL837525.5 and AL671882.6. On Mar 21, 2009 this sequence version replaced NM_016693.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB021861.4, BC120565.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGC CATGTCCTGG GGCCGGGGTT CTAGAACGGG CGGGCAGCTG CTGGCAGGAC 
CCGCTGGCGG AGGCGCTGAG CCGGGGCCGC TCGTCTCCTG CGGTCACGGG TCGGGGCTGC 
GCGCGGAGCC GGCCTCTCAG TGTGGTCTAC GTGCTGACCC GGGAGCCGGG GCTCGGGGTG 
GAGCCCGGGT CGGGAACCGA GGCAGAGCCG CTGCCGTTGC GATGCCTGCG CGAGGCCTGC 
GCTCAGCTCC AGGGCACACG GCCACCCCCG CAGCTACGCA GCCTACCCTT TGCGACGCTG 
GCGCTAGGCG ACACCGCTGC GCTGGACTCC TTCTACAACG CGGATGTAGT GGTGCTGGAG 
GTGAGCAGCT CCCTGGCACA GCCTTCCTTG TTCTACCACC TCGGCGTGCG TGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGTTCC CAGGCAGAGC TCCCTGATCT GCAGGCCCTT 
CGTGAGGATG TTTTCCAGAA GAACTCGGAT TGTGTTGGCA GCTACACACT GATTCCTTAT 
GTGGTGACAG CCACTGGCCG GGTCTTATGT GGCGATGCAG GCCTCCTGAG GGGCATAGCC 
GATGGGCTAG TACAGGCTGG GGCGGGCACT GAAGCCCTCC TCACTCCCCT GGTGGGCCGG 
CTTGTCCGTC TGTTGGAGGC TACACCCACA GACTCTTGCG GCTATTTCCG GGAGACCATT 
CGTCAGGATA TCCGGCAGGC TCGGGAGCGA TTCAGTGGGC AGCAGCTGAG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGGCT GGACAGCGTG GAGCTGCTGA GCCCTGACAT TGTCATGAAT 
CTGCTGCTAT CGTACCGTGA TGTCCAGGAC TACTCGGCCA TCATTGAGCT GGTGGAGACG 
CTGCAGGCTT TGCCCACCTG TGATGTGGCT GAACAGCACA ATGTCTGCTT TCACTACACA 
TTTGCCCTCA ACAGGAGGAA CAGGCCTGGG GACCGGGAGA AAGCCCTGGC TGTCCTGCTG 
CCATTGGTGA AGTATGAAGG TCCCGTGGCT CCGGATCTGT ACTGCATGTG TGGTCGTATC 
TACAAGGACA TGTTCTTCAC TTCTGGCTTC CAAAATGCCG GCCACCTGGA GCAGGCCTAT 
CACTGGTACC GCAAGGCGTT TGATGTGGAG CCCAGCCTGC ACTCAGGCAT CAATGCAGCT 
GTTCTCCTCA TTGCAGCTGG GCAGCATTTC GAAGACTCTG AGGAGCTCCG GCTGATTGGC 
ATGAAGCTGG CCTGCCTGCT AGCCCGCAAA GGCTGTGTGG AGAAGATGCA GTACTACTGG 
GATGTGGGAT TCTACCTGGG AGCCCAGATT CTTGCCAACG ACCCCATCCA GGTGGTGCTG 
GCTGCAGAGC AACTGTACAA GCTTAATGCC CCCATTTGGT ACTTGGTCTC CGTGATGGAG 
ACCTTCCTAC TGTACCAGCA TTTCAGACCC ACACCAGAGC CCTCAGGAGG ACCCCCGCTG 
CGAGCTCACT TCTGGCTCCA CTTCCTGCTA CAATCTTGCC AGCCTTTCAA GATGGCTCCT 
CCTCAGGAGG ACCAGTGCCT GGTACTGGTG CTGGAGATAA ACAAGGTCCT GCTGCCTGCC 
AGGCTTGAGA TTCAGGGAAC AGACCCCGTG AGCACAGTGA CCCTAAGCTT GCTGGACCCA 
GAGACTCAGG AGGATCCTTC CAGCTGGACC TTCCCAGTCA CCTCCATCTG TGGGATCAGC 
ACCTCCAAGC TGGACCAGCG CTGCTGCTTC CTGTACGCAC TTCCTCCGGC CCAGGACGTC 
CAGCTGTGCT TTCCCAGTGT GGAGCGCTGC CAGCGGTTCT GTGGCCTAAT TCAGGTCTTG 
GTGATGAATC CGGATTCCTC CGCGCCCACT GAGGAGGCAG AAGGCGCAAG GGAGGTGCTG 
GAGTTTGATT ATGAATACTC GGAGACTGGT GAGCGGCTGG TGTTGGGCAG AGGCACGTAT 
GGGGTGGTGT ATGCTGGCCG AGACAGGCAC ACGAGGGTAC GAATCGCCAT CAAGGAGATC 
CCAGAGAGAG ATAGCAGATT CTCTCAGCCT CTGCATGAAG AGATCGCTCT TCACAAACGA 
CTTCGTCATA AGAATATTGT GCGTTATCTG GGCTCAGCCA GCCAGGGCGG CTACCTCAAG 
ATCTTCATGG AGGAAGTGCC TGGAGGCAGC CTGTCCTCCT TGCTTAGGTC AGTGTGGGGA 
CCCCTAAAGG ACAACGAGAG CACTATTAGC TTCTATACAC GTCAGATCCT ACAAGGACTC 
AGCTACCTCC ATGAGAACCG TATCGTTCAC AGAGACATCA AGGGAGACAA TGTTCTGATC 
AACACCTTCA GTGGGCTGCT CAAGATTTCT GACTTTGGCA CCTCTAAGCG GCTGGCAGGC 
ATCACACCGT GCACGGAGAC TTTCACAGGG ACTCTGCAGT ATATGGCCCC GGAAATCATC 
GACCAGGGCC CGCGAGGATA TGGGAAGGCA GCTGACATCT GGTCTCTGGG CTGCACTGTA 
ATCGAGATGG CAACAGGTCG ACCGCCCTTC CATGAACTAG GGAGCCCTCA GGCTGCGATG 
TTTCAGGTGG GCATGTACAA GGTACATCCA CCCGTGCCCG GTTCCCTGTC AGCTGAGGCC 
CAAGCCTTCC TCCTCCGAAC TTTTGAGCCA GATCCCCGCC TCCGAGCCAG TGCCCAAGAG 
CTGCTGGGAG ACCCCTTCCT TCAGCCAGGA AAGAGGAGCC GCAGCCCGGG CTCTCCTCGG 
CATACTCCCC GGCCCTCAGG CGCCCCTTCC GGTCCTTCCA GTCCTTCAGC TGACTCAGCC 
ACACAGTCTC AGACATTCCC AAGGCCCCAG GCACCCTCTC AGCACCCACC CAGCCCCCCA 
AAGCGCTGCC TTAGTTATGG GGACACCAGT CAGCTCCGTG TGCCCGAGGA GCCCGCTGCC 
GAGGAACCCG CGTCCCCAGA AGAGAGTTCA GGCCTGAGTC TGCTGCACCA GGAGAGCAAG 
CGCCGGGCCA TGCTGGCTGC GGTGCTGGAA CAGGAGGTGC CCACACTAGC AGAGAATCTC 
CTGGAACAGG AACAGGACTC TCGACTCAGC AAGATTCATG TGGAACTTCT GCTTCGGTGC 
CTTGGGGCAC AAATCCACAC TCCTAACCGC CGGCAGCTGG CCCAGGAGCT GCGGACCCTG 
CAAGCTCAGC TGCGGGCCCA GAGCCTGGGC CCTGCGCTTT TGAAGGGGCC GCTCTTCGCC 
TTTCCAGACG CGGTGAAGCA GATCCTCCGC AGACGCCAGA TCCGCCCACA CTGGATGTTC 
GTGTTGGACT CGCTGCTCAG CCGTGCAGTC CGGGCGGCGA TGGCGGTGCT GGACGCGGAG 
TCGGAGAAGA AAGCGGTCTT ACCGAGGTCA GAAGAGTCGA GTAAAGAGGA GTCCCAGCAG 
AAACCTCAGG AGAGCCAGGC GCTGCAGAGC CAGCTCCCAC CAGAGCAGGG ACCCCCATCG 
TTGATGGTGG AGTTGGGCCT TTTGCGAGCC GAGACTGACA GGCTTCGGGA CCTTCTGGCT 
GAGAAGGAAC GTGAGTGCCA GGCGCTGGTG CAACAGGCCC TGCATCGGGT GCATGCAGAG 
ACCAGGAAGT ATGCCCCAGC TTCAGAGACC CCAGCCACTC TCCCAAAGGA CCAGAACCTG 
GTGCGGTGGC TACAGGAACT GAGTGTAGAT CCAGCCACTA TCCAAACGCT CCTGAGTCAT 
AGCTTCACCC TTCAAACCCT GCTCACCTGT GCCACTCAAG ATGATCTAGT GTACACCAGA 
ATCAGGGGAG GGATGGTATG CCGGATTTGG AGAGCCATCT TGGCACAGCG AGCAGGAGCC 
ACATCGGTCA CCCCAGTACC CCGGGACGCT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057902.5
CDS	272..4147
Translation	MAGPCPGAGVLERAGSCWQDPLAEALSRGRSSPAVTGRGCARSRPLSVVYVLTREPGLGVEPGSGTEAEPLPLRCLREACAQLQGTRPPPQLRSLPFATLALGDTAALDSFYNADVVVLEVSSSLAQPSLFYHLGVRESFSMTNNVLLCSQAELPDLQALREDVFQKNSDCVGSYTLIPYVVTATGRVLCGDAGLLRGIADGLVQAGAGTEALLTPLVGRLVRLLEATPTDSCGYFRETIRQDIRQARERFSGQQLRQELARLQRRLDSVELLSPDIVMNLLLSYRDVQDYSAIIELVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDREKALAVLLPLVKYEGPVAPDLYCMCGRIYKDMFFTSGFQNAGHLEQAYHWYRKAFDVEPSLHSGINAAVLLIAAGQHFEDSEELRLIGMKLACLLARKGCVEKMQYYWDVGFYLGAQILANDPIQVVLAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTPEPSGGPPLRAHFWLHFLLQSCQPFKMAPPQEDQCLVLVLEINKVLLPARLEIQGTDPVSTVTLSLLDPETQEDPSSWTFPVTSICGISTSKLDQRCCFLYALPPAQDVQLCFPSVERCQRFCGLIQVLVMNPDSSAPTEEAEGAREVLEFDYEYSETGERLVLGRGTYGVVYAGRDRHTRVRIAIKEIPERDSRFSQPLHEEIALHKRLRHKNIVRYLGSASQGGYLKIFMEEVPGGSLSSLLRSVWGPLKDNESTISFYTRQILQGLSYLHENRIVHRDIKGDNVLINTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGRPPFHELGSPQAAMFQVGMYKVHPPVPGSLSAEAQAFLLRTFEPDPRLRASAQELLGDPFLQPGKRSRSPGSPRHTPRPSGAPSGPSSPSADSATQSQTFPRPQAPSQHPPSPPKRCLSYGDTSQLRVPEEPAAEEPASPEESSGLSLLHQESKRRAMLAAVLEQEVPTLAENLLEQEQDSRLSKIHVELLLRCLGAQIHTPNRRQLAQELRTLQAQLRAQSLGPALLKGPLFAFPDAVKQILRRRQIRPHWMFVLDSLLSRAVRAAMAVLDAESEKKAVLPRSEESSKEESQQKPQESQALQSQLPPEQGPPSLMVELGLLRAETDRLRDLLAEKERECQALVQQALHRVHAETRKYAPASETPATLPKDQNLVRWLQELSVDPATIQTLLSHSFTLQTLLTCATQDDLVYTRIRGGMVCRIWRAILAQRAGATSVTPVPRDAE

Target ORF information:

RefSeq Version	NM_016693.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016693.5

ORF Insert Sequence:

ATGGCGGGGC CATGTCCTGG GGCCGGGGTT CTAGAACGGG CGGGCAGCTG CTGGCAGGAC 
CCGCTGGCGG AGGCGCTGAG CCGGGGCCGC TCGTCTCCTG CGGTCACGGG TCGGGGCTGC 
GCGCGGAGCC GGCCTCTCAG TGTGGTCTAC GTGCTGACCC GGGAGCCGGG GCTCGGGGTG 
GAGCCCGGGT CGGGAACCGA GGCAGAGCCG CTGCCGTTGC GATGCCTGCG CGAGGCCTGC 
GCTCAGCTCC AGGGCACACG GCCACCCCCG CAGCTACGCA GCCTACCCTT TGCGACGCTG 
GCGCTAGGCG ACACCGCTGC GCTGGACTCC TTCTACAACG CGGATGTAGT GGTGCTGGAG 
GTGAGCAGCT CCCTGGCACA GCCTTCCTTG TTCTACCACC TCGGCGTGCG TGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGTTCC CAGGCAGAGC TCCCTGATCT GCAGGCCCTT 
CGTGAGGATG TTTTCCAGAA GAACTCGGAT TGTGTTGGCA GCTACACACT GATTCCTTAT 
GTGGTGACAG CCACTGGCCG GGTCTTATGT GGCGATGCAG GCCTCCTGAG GGGCATAGCC 
GATGGGCTAG TACAGGCTGG GGCGGGCACT GAAGCCCTCC TCACTCCCCT GGTGGGCCGG 
CTTGTCCGTC TGTTGGAGGC TACACCCACA GACTCTTGCG GCTATTTCCG GGAGACCATT 
CGTCAGGATA TCCGGCAGGC TCGGGAGCGA TTCAGTGGGC AGCAGCTGAG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGGCT GGACAGCGTG GAGCTGCTGA GCCCTGACAT TGTCATGAAT 
CTGCTGCTAT CGTACCGTGA TGTCCAGGAC TACTCGGCCA TCATTGAGCT GGTGGAGACG 
CTGCAGGCTT TGCCCACCTG TGATGTGGCT GAACAGCACA ATGTCTGCTT TCACTACACA 
TTTGCCCTCA ACAGGAGGAA CAGGCCTGGG GACCGGGAGA AAGCCCTGGC TGTCCTGCTG 
CCATTGGTGA AGTATGAAGG TCCCGTGGCT CCGGATCTGT ACTGCATGTG TGGTCGTATC 
TACAAGGACA TGTTCTTCAC TTCTGGCTTC CAAAATGCCG GCCACCTGGA GCAGGCCTAT 
CACTGGTACC GCAAGGCGTT TGATGTGGAG CCCAGCCTGC ACTCAGGCAT CAATGCAGCT 
GTTCTCCTCA TTGCAGCTGG GCAGCATTTC GAAGACTCTG AGGAGCTCCG GCTGATTGGC 
ATGAAGCTGG CCTGCCTGCT AGCCCGCAAA GGCTGTGTGG AGAAGATGCA GTACTACTGG 
GATGTGGGAT TCTACCTGGG AGCCCAGATT CTTGCCAACG ACCCCATCCA GGTGGTGCTG 
GCTGCAGAGC AACTGTACAA GCTTAATGCC CCCATTTGGT ACTTGGTCTC CGTGATGGAG 
ACCTTCCTAC TGTACCAGCA TTTCAGACCC ACACCAGAGC CCTCAGGAGG ACCCCCGCTG 
CGAGCTCACT TCTGGCTCCA CTTCCTGCTA CAATCTTGCC AGCCTTTCAA GATGGCTCCT 
CCTCAGGAGG ACCAGTGCCT GGTACTGGTG CTGGAGATAA ACAAGGTCCT GCTGCCTGCC 
AGGCTTGAGA TTCAGGGAAC AGACCCCGTG AGCACAGTGA CCCTAAGCTT GCTGGACCCA 
GAGACTCAGG AGGATCCTTC CAGCTGGACC TTCCCAGTCA CCTCCATCTG TGGGATCAGC 
ACCTCCAAGC TGGACCAGCG CTGCTGCTTC CTGTACGCAC TTCCTCCGGC CCAGGACGTC 
CAGCTGTGCT TTCCCAGTGT GGAGCGCTGC CAGCGGTTCT GTGGCCTAAT TCAGGTCTTG 
GTGATGAATC CGGATTCCTC CGCGCCCACT GAGGAGGCAG AAGGCGCAAG GGAGGTGCTG 
GAGTTTGATT ATGAATACTC GGAGACTGGT GAGCGGCTGG TGTTGGGCAG AGGCACGTAT 
GGGGTGGTGT ATGCTGGCCG AGACAGGCAC ACGAGGGTAC GAATCGCCAT CAAGGAGATC 
CCAGAGAGAG ATAGCAGATT CTCTCAGCCT CTGCATGAAG AGATCGCTCT TCACAAACGA 
CTTCGTCATA AGAATATTGT GCGTTATCTG GGCTCAGCCA GCCAGGGCGG CTACCTCAAG 
ATCTTCATGG AGGAAGTGCC TGGAGGCAGC CTGTCCTCCT TGCTTAGGTC AGTGTGGGGA 
CCCCTAAAGG ACAACGAGAG CACTATTAGC TTCTATACAC GTCAGATCCT ACAAGGACTC 
AGCTACCTCC ATGAGAACCG TATCGTTCAC AGAGACATCA AGGGAGACAA TGTTCTGATC 
AACACCTTCA GTGGGCTGCT CAAGATTTCT GACTTTGGCA CCTCTAAGCG GCTGGCAGGC 
ATCACACCGT GCACGGAGAC TTTCACAGGG ACTCTGCAGT ATATGGCCCC GGAAATCATC 
GACCAGGGCC CGCGAGGATA TGGGAAGGCA GCTGACATCT GGTCTCTGGG CTGCACTGTA 
ATCGAGATGG CAACAGGTCG ACCGCCCTTC CATGAACTAG GGAGCCCTCA GGCTGCGATG 
TTTCAGGTGG GCATGTACAA GGTACATCCA CCCGTGCCCG GTTCCCTGTC AGCTGAGGCC 
CAAGCCTTCC TCCTCCGAAC TTTTGAGCCA GATCCCCGCC TCCGAGCCAG TGCCCAAGAG 
CTGCTGGGAG ACCCCTTCCT TCAGCCAGGA AAGAGGAGCC GCAGCCCGGG CTCTCCTCGG 
CATACTCCCC GGCCCTCAGG CGCCCCTTCC GGTCCTTCCA GTCCTTCAGC TGACTCAGCC 
ACACAGTCTC AGACATTCCC AAGGCCCCAG GCACCCTCTC AGCACCCACC CAGCCCCCCA 
AAGCGCTGCC TTAGTTATGG GGACACCAGT CAGCTCCGTG TGCCCGAGGA GCCCGCTGCC 
GAGGAACCCG CGTCCCCAGA AGAGAGTTCA GGCCTGAGTC TGCTGCACCA GGAGAGCAAG 
CGCCGGGCCA TGCTGGCTGC GGTGCTGGAA CAGGAGGTGC CCACACTAGC AGAGAATCTC 
CTGGAACAGG AACAGGACTC TCGACTCAGC AAGATTCATG TGGAACTTCT GCTTCGGTGC 
CTTGGGGCAC AAATCCACAC TCCTAACCGC CGGCAGCTGG CCCAGGAGCT GCGGACCCTG 
CAAGCTCAGC TGCGGGCCCA GAGCCTGGGC CCTGCGCTTT TGAAGGGGCC GCTCTTCGCC 
TTTCCAGACG CGGTGAAGCA GATCCTCCGC AGACGCCAGA TCCGCCCACA CTGGATGTTC 
GTGTTGGACT CGCTGCTCAG CCGTGCAGTC CGGGCGGCGA TGGCGGTGCT GGACGCGGAG 
TCGGAGAAGA AAGCGGTCTT ACCGAGGTCA GAAGAGTCGA GTAAAGAGGA GTCCCAGCAG 
AAACCTCAGG AGAGCCAGGC GCTGCAGAGC CAGCTCCCAC CAGAGCAGGG ACCCCCATCG 
TTGATGGTGG AGTTGGGCCT TTTGCGAGCC GAGACTGACA GGCTTCGGGA CCTTCTGGCT 
GAGAAGGAAC GTGAGTGCCA GGCGCTGGTG CAACAGGCCC TGCATCGGGT GCATGCAGAG 
ACCAGGAAGT ATGCCCCAGC TTCAGAGACC CCAGCCACTC TCCCAAAGGA CCAGAACCTG 
GTGCGGTGGC TACAGGAACT GAGTGTAGAT CCAGCCACTA TCCAAACGCT CCTGAGTCAT 
AGCTTCACCC TTCAAACCCT GCTCACCTGT GCCACTCAAG ATGATCTAGT GTACACCAGA 
ATCAGGGGAG GGATGGTATG CCGGATTTGG AGAGCCATCT TGGCACAGCG AGCAGGAGCC 
ACATCGGTCA CCCCAGTACC CCGGGACGCT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k6 ( XM_006539040.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539103.1 Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), transcript variant X1, mRNA.

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CloneID	OMu34336
Clone ID Related Accession (Same CDS sequence)	XM_006539040.4 , XM_006539040.3
Accession Version	XM_006539040.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539040.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC CATGTCCTGG GGCCGGGGTT CTAGAACGGG CGGGCAGCTG CTGGCAGGAC 
CCGCTGGCGG AGGCGCTGAG CCGGGGCCGC TCGTCTCCTG CGGTCACGGG TCGGGGCTGC 
GCGCGGAGCC GGCCTCTCAG TGTGGTCTAC GTGCTGACCC GGGAGCCGGG GCTCGGGGTG 
GAGCCCGGGT CGGGAACCGA GGCAGAGCCG CTGCCGTTGC GATGCCTGCG CGAGGCCTGC 
GCTCAGCTCC AGGGCACACG GCCACCCCCG CAGCTACGCA GCCTACCCTT TGCGACGCTG 
GCGCTAGGCG ACACCGCTGC GCTGGACTCC TTCTACAACG CGGATGTAGT GGTGCTGGAG 
GTGAGCAGCT CCCTGGCACA GCCTTCCTTG TTCTACCACC TCGGCGTGCG TGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGTTCC CAGGCAGAGC TCCCTGATCT GCAGGCCCTT 
CGTGATTGTG TTGGCAGCTA CACACTGATT CCTTATGTGG TGACAGCCAC TGGCCGGGTC 
TTATGTGGCG ATGCAGGCCT CCTGAGGGGC ATAGCCGATG GGCTAGTACA GGCTGGGGCG 
GGCACTGAAG CCCTCCTCAC TCCCCTGGTG GGCCGGCTTG TCCGTCTGTT GGAGGCTACA 
CCCACAGACT CTTGCGGCTA TTTCCGGGAG ACCATTCGTC AGGATATCCG GCAGGCTCGG 
GAGCGATTCA GTGGGCAGCA GCTGAGGCAG GAGCTGGCTC GCCTGCAGCG GAGGCTGGAC 
AGCGTGGAGC TGCTGAGCCC TGACATTGTC ATGAATCTGC TGCTATCGTA CCGTGATGTC 
CAGGACTACT CGGCCATCAT TGAGCTGGTG GAGACGCTGC AGGCTTTGCC CACCTGTGAT 
GTGGCTGAAC AGCACAATGT CTGCTTTCAC TACACATTTG CCCTCAACAG GAGGAACAGG 
CCTGGGGACC GGGAGAAAGC CCTGGCTGTC CTGCTGCCAT TGGTGAAGTA TGAAGGTCCC 
GTGGCTCCGG ATCTGTACTG CATGTGTGGT CGTATCTACA AGGACATGTT CTTCACTTCT 
GGCTTCCAAA ATGCCGGCCA CCTGGAGCAG GCCTATCACT GGTACCGCAA GGCGTTTGAT 
GTGGAGCCCA GCCTGCACTC AGGCATCAAT GCAGCTGTTC TCCTCATTGC AGCTGGGCAG 
CATTTCGAAG ACTCTGAGGA GCTCCGGCTG ATTGGCATGA AGCTGGCCTG CCTGCTAGCC 
CGCAAAGGCT GTGTGGAGAA GATGCAGTAC TACTGGGATG TGGGATTCTA CCTGGGAGCC 
CAGATTCTTG CCAACGACCC CATCCAGGTG GTGCTGGCTG CAGAGCAACT GTACAAGCTT 
AATGCCCCCA TTTGGTACTT GGTCTCCGTG ATGGAGACCT TCCTACTGTA CCAGCATTTC 
AGACCCACAC CAGAGCCCTC AGGAGGACCC CCGCTGCGAG CTCACTTCTG GCTCCACTTC 
CTGCTACAAT CTTGCCAGCC TTTCAAGATG GCTCCTCCTC AGGAGGACCA GTGCCTGGTA 
CTGGTGCTGG AGATAAACAA GGTCCTGCTG CCTGCCAGGC TTGAGATTCA GGGAACAGAC 
CCCGTGAGCA CAGTGACCCT AAGCTTGCTG GACCCAGAGA CTCAGGAGGA TCCTTCCAGC 
TGGACCTTCC CAGTCACCTC CATCTGTGGG ATCAGCACCT CCAAGCTGGA CCAGCGCTGC 
TGCTTCCTGT ACGCACTTCC TCCGGCCCAG GACGTCCAGC TGTGCTTTCC CAGTGTGGAG 
CGCTGCCAGC GGTTCTGTGG CCTAATTCAG GTCTTGGTGA TGAATCCGGA TTCCTCCGCG 
CCCACTGAGG AGGCAGAAGG CGCAAGGGAG GTGCTGGAGT TTGATTATGA ATACTCGGAG 
ACTGGTGAGC GGCTGGTGTT GGGCAGAGGC ACGTATGGGG TGGTGTATGC TGGCCGAGAC 
AGGCACACGA GGGTACGAAT CGCCATCAAG GAGATCCCAG AGAGAGATAG CAGATTCTCT 
CAGCCTCTGC ATGAAGAGAT CGCTCTTCAC AAACGACTTC GTCATAAGAA TATTGTGCGT 
TATCTGGGCT CAGCCAGCCA GGGCGGCTAC CTCAAGATCT TCATGGAGGA AGTGCCTGGA 
GGCAGCCTGT CCTCCTTGCT TAGGTCAGTG TGGGGACCCC TAAAGGACAA CGAGAGCACT 
ATTAGCTTCT ATACACGTCA GATCCTACAA GGACTCAGCT ACCTCCATGA GAACCGTATC 
GTTCACAGAG ACATCAAGGG AGACAATGTT CTGATCAACA CCTTCAGTGG GCTGCTCAAG 
ATTTCTGACT TTGGCACCTC TAAGCGGCTG GCAGGCATCA CACCGTGCAC GGAGACTTTC 
ACAGGGACTC TGCAGTATAT GGCCCCGGAA ATCATCGACC AGGGCCCGCG AGGATATGGG 
AAGGCAGCTG ACATCTGGTC TCTGGGCTGC ACTGTAATCG AGATGGCAAC AGGTCGACCG 
CCCTTCCATG AACTAGGGAG CCCTCAGGCT GCGATGTTTC AGGTGGGCAT GTACAAGGTA 
CATCCACCCG TGCCCGGTTC CCTGTCAGCT GAGGCCCAAG CCTTCCTCCT CCGAACTTTT 
GAGCCAGATC CCCGCCTCCG AGCCAGTGCC CAAGAGCTGC TGGGAGACCC CTTCCTTCAG 
CCAGGAAAGA GGAGCCGCAG CCCGGGCTCT CCTCGGCATA CTCCCCGGCC CTCAGGCGCC 
CCTTCCGGTC CTTCCAGTCC TTCAGCTGAC TCAGCCACAC AGTCTCAGAC ATTCCCAAGG 
CCCCAGGCAC CCTCTCAGCA CCCACCCAGC CCCCCAAAGC GCTGCCTTAG TTATGGGGAC 
ACCAGTCAGC TCCGTGTGCC CGAGGAGCCC GCTGCCGAGG AACCCGCGTC CCCAGAAGAG 
AGTTCAGGCC TGAGTCTGCT GCACCAGGAG AGCAAGCGCC GGGCCATGCT GGCTGCGGTG 
CTGGAACAGG AGGTGCCCAC ACTAGCAGAG AATCTCCTGG AACAGGAACA GGACTCTCGA 
CTCAGCAAGA TTCATGTGGA ACTTCTGCTT CGGTGCCTTG GGGCACAAAT CCACACTCCT 
AACCGCCGGC AGCTGGCCCA GGAGCTGCGG ACCCTGCAAG CTCAGCTGCG GGCCCAGAGC 
CTGGGCCCTG CGCTTTTGAA GGGGCCGCTC TTCGCCTTTC CAGACGCGGT GAAGCAGATC 
CTCCGCAGAC GCCAGATCCG CCCACACTGG ATGTTCGTGT TGGACTCGCT GCTCAGCCGT 
GCAGTCCGGG CGGCGATGGC GGTGCTGGAC GCGGAGTCGG AGAAGAAAGC GGTCTTACCG 
AGGTCAGAAG AGTCGAGTAA AGAGGAGTCC CAGCAGAAAC CTCAGGAGAG CCAGGCGCTG 
CAGAGCCAGC TCCCACCAGA GCAGGGACCC CCATCGTTGA TGGTGGAGTT GGGCCTTTTG 
CGAGCCGAGA CTGACAGGCT TCGGGACCTT CTGGCTGAGA AGGAACGTGA GTGCCAGGCG 
CTGGTGCAAC AGGCCCTGCA TCGGGTGCAT GCAGAGACCA GGAAGTATGC CCCAGCTTCA 
GAGACCCCAG CCACTCTCCC AAAGGACCAG AACCTGGTGC GGTGGCTACA GGAACTGAGT 
GTAGATCCAG CCACTATCCA AACGCTCCTG AGTCATAGCT TCACCCTTCA AACCCTGCTC 
ACCTGTGCCA CTCAAGATGA TCTAGTGTAC ACCAGAATCA GGGGAGGGAT GGTATGCCGG 
ATTTGGAGAG CCATCTTGGC ACAGCGAGCA GGAGCCACAT CGGTCACCCC AGTACCCCGG 
GACGCTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539103.1
CDS	1427..5278
Translation	MAGPCPGAGVLERAGSCWQDPLAEALSRGRSSPAVTGRGCARSRPLSVVYVLTREPGLGVEPGSGTEAEPLPLRCLREACAQLQGTRPPPQLRSLPFATLALGDTAALDSFYNADVVVLEVSSSLAQPSLFYHLGVRESFSMTNNVLLCSQAELPDLQALRDCVGSYTLIPYVVTATGRVLCGDAGLLRGIADGLVQAGAGTEALLTPLVGRLVRLLEATPTDSCGYFRETIRQDIRQARERFSGQQLRQELARLQRRLDSVELLSPDIVMNLLLSYRDVQDYSAIIELVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDREKALAVLLPLVKYEGPVAPDLYCMCGRIYKDMFFTSGFQNAGHLEQAYHWYRKAFDVEPSLHSGINAAVLLIAAGQHFEDSEELRLIGMKLACLLARKGCVEKMQYYWDVGFYLGAQILANDPIQVVLAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTPEPSGGPPLRAHFWLHFLLQSCQPFKMAPPQEDQCLVLVLEINKVLLPARLEIQGTDPVSTVTLSLLDPETQEDPSSWTFPVTSICGISTSKLDQRCCFLYALPPAQDVQLCFPSVERCQRFCGLIQVLVMNPDSSAPTEEAEGAREVLEFDYEYSETGERLVLGRGTYGVVYAGRDRHTRVRIAIKEIPERDSRFSQPLHEEIALHKRLRHKNIVRYLGSASQGGYLKIFMEEVPGGSLSSLLRSVWGPLKDNESTISFYTRQILQGLSYLHENRIVHRDIKGDNVLINTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGRPPFHELGSPQAAMFQVGMYKVHPPVPGSLSAEAQAFLLRTFEPDPRLRASAQELLGDPFLQPGKRSRSPGSPRHTPRPSGAPSGPSSPSADSATQSQTFPRPQAPSQHPPSPPKRCLSYGDTSQLRVPEEPAAEEPASPEESSGLSLLHQESKRRAMLAAVLEQEVPTLAENLLEQEQDSRLSKIHVELLLRCLGAQIHTPNRRQLAQELRTLQAQLRAQSLGPALLKGPLFAFPDAVKQILRRRQIRPHWMFVLDSLLSRAVRAAMAVLDAESEKKAVLPRSEESSKEESQQKPQESQALQSQLPPEQGPPSLMVELGLLRAETDRLRDLLAEKERECQALVQQALHRVHAETRKYAPASETPATLPKDQNLVRWLQELSVDPATIQTLLSHSFTLQTLLTCATQDDLVYTRIRGGMVCRIWRAILAQRAGATSVTPVPRDAE

Target ORF information:

RefSeq Version	XM_006539040.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 6 (Map3k6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539040.3

ORF Insert Sequence:

ATGGCGGGGC CATGTCCTGG GGCCGGGGTT CTAGAACGGG CGGGCAGCTG CTGGCAGGAC 
CCGCTGGCGG AGGCGCTGAG CCGGGGCCGC TCGTCTCCTG CGGTCACGGG TCGGGGCTGC 
GCGCGGAGCC GGCCTCTCAG TGTGGTCTAC GTGCTGACCC GGGAGCCGGG GCTCGGGGTG 
GAGCCCGGGT CGGGAACCGA GGCAGAGCCG CTGCCGTTGC GATGCCTGCG CGAGGCCTGC 
GCTCAGCTCC AGGGCACACG GCCACCCCCG CAGCTACGCA GCCTACCCTT TGCGACGCTG 
GCGCTAGGCG ACACCGCTGC GCTGGACTCC TTCTACAACG CGGATGTAGT GGTGCTGGAG 
GTGAGCAGCT CCCTGGCACA GCCTTCCTTG TTCTACCACC TCGGCGTGCG TGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGTTCC CAGGCAGAGC TCCCTGATCT GCAGGCCCTT 
CGTGATTGTG TTGGCAGCTA CACACTGATT CCTTATGTGG TGACAGCCAC TGGCCGGGTC 
TTATGTGGCG ATGCAGGCCT CCTGAGGGGC ATAGCCGATG GGCTAGTACA GGCTGGGGCG 
GGCACTGAAG CCCTCCTCAC TCCCCTGGTG GGCCGGCTTG TCCGTCTGTT GGAGGCTACA 
CCCACAGACT CTTGCGGCTA TTTCCGGGAG ACCATTCGTC AGGATATCCG GCAGGCTCGG 
GAGCGATTCA GTGGGCAGCA GCTGAGGCAG GAGCTGGCTC GCCTGCAGCG GAGGCTGGAC 
AGCGTGGAGC TGCTGAGCCC TGACATTGTC ATGAATCTGC TGCTATCGTA CCGTGATGTC 
CAGGACTACT CGGCCATCAT TGAGCTGGTG GAGACGCTGC AGGCTTTGCC CACCTGTGAT 
GTGGCTGAAC AGCACAATGT CTGCTTTCAC TACACATTTG CCCTCAACAG GAGGAACAGG 
CCTGGGGACC GGGAGAAAGC CCTGGCTGTC CTGCTGCCAT TGGTGAAGTA TGAAGGTCCC 
GTGGCTCCGG ATCTGTACTG CATGTGTGGT CGTATCTACA AGGACATGTT CTTCACTTCT 
GGCTTCCAAA ATGCCGGCCA CCTGGAGCAG GCCTATCACT GGTACCGCAA GGCGTTTGAT 
GTGGAGCCCA GCCTGCACTC AGGCATCAAT GCAGCTGTTC TCCTCATTGC AGCTGGGCAG 
CATTTCGAAG ACTCTGAGGA GCTCCGGCTG ATTGGCATGA AGCTGGCCTG CCTGCTAGCC 
CGCAAAGGCT GTGTGGAGAA GATGCAGTAC TACTGGGATG TGGGATTCTA CCTGGGAGCC 
CAGATTCTTG CCAACGACCC CATCCAGGTG GTGCTGGCTG CAGAGCAACT GTACAAGCTT 
AATGCCCCCA TTTGGTACTT GGTCTCCGTG ATGGAGACCT TCCTACTGTA CCAGCATTTC 
AGACCCACAC CAGAGCCCTC AGGAGGACCC CCGCTGCGAG CTCACTTCTG GCTCCACTTC 
CTGCTACAAT CTTGCCAGCC TTTCAAGATG GCTCCTCCTC AGGAGGACCA GTGCCTGGTA 
CTGGTGCTGG AGATAAACAA GGTCCTGCTG CCTGCCAGGC TTGAGATTCA GGGAACAGAC 
CCCGTGAGCA CAGTGACCCT AAGCTTGCTG GACCCAGAGA CTCAGGAGGA TCCTTCCAGC 
TGGACCTTCC CAGTCACCTC CATCTGTGGG ATCAGCACCT CCAAGCTGGA CCAGCGCTGC 
TGCTTCCTGT ACGCACTTCC TCCGGCCCAG GACGTCCAGC TGTGCTTTCC CAGTGTGGAG 
CGCTGCCAGC GGTTCTGTGG CCTAATTCAG GTCTTGGTGA TGAATCCGGA TTCCTCCGCG 
CCCACTGAGG AGGCAGAAGG CGCAAGGGAG GTGCTGGAGT TTGATTATGA ATACTCGGAG 
ACTGGTGAGC GGCTGGTGTT GGGCAGAGGC ACGTATGGGG TGGTGTATGC TGGCCGAGAC 
AGGCACACGA GGGTACGAAT CGCCATCAAG GAGATCCCAG AGAGAGATAG CAGATTCTCT 
CAGCCTCTGC ATGAAGAGAT CGCTCTTCAC AAACGACTTC GTCATAAGAA TATTGTGCGT 
TATCTGGGCT CAGCCAGCCA GGGCGGCTAC CTCAAGATCT TCATGGAGGA AGTGCCTGGA 
GGCAGCCTGT CCTCCTTGCT TAGGTCAGTG TGGGGACCCC TAAAGGACAA CGAGAGCACT 
ATTAGCTTCT ATACACGTCA GATCCTACAA GGACTCAGCT ACCTCCATGA GAACCGTATC 
GTTCACAGAG ACATCAAGGG AGACAATGTT CTGATCAACA CCTTCAGTGG GCTGCTCAAG 
ATTTCTGACT TTGGCACCTC TAAGCGGCTG GCAGGCATCA CACCGTGCAC GGAGACTTTC 
ACAGGGACTC TGCAGTATAT GGCCCCGGAA ATCATCGACC AGGGCCCGCG AGGATATGGG 
AAGGCAGCTG ACATCTGGTC TCTGGGCTGC ACTGTAATCG AGATGGCAAC AGGTCGACCG 
CCCTTCCATG AACTAGGGAG CCCTCAGGCT GCGATGTTTC AGGTGGGCAT GTACAAGGTA 
CATCCACCCG TGCCCGGTTC CCTGTCAGCT GAGGCCCAAG CCTTCCTCCT CCGAACTTTT 
GAGCCAGATC CCCGCCTCCG AGCCAGTGCC CAAGAGCTGC TGGGAGACCC CTTCCTTCAG 
CCAGGAAAGA GGAGCCGCAG CCCGGGCTCT CCTCGGCATA CTCCCCGGCC CTCAGGCGCC 
CCTTCCGGTC CTTCCAGTCC TTCAGCTGAC TCAGCCACAC AGTCTCAGAC ATTCCCAAGG 
CCCCAGGCAC CCTCTCAGCA CCCACCCAGC CCCCCAAAGC GCTGCCTTAG TTATGGGGAC 
ACCAGTCAGC TCCGTGTGCC CGAGGAGCCC GCTGCCGAGG AACCCGCGTC CCCAGAAGAG 
AGTTCAGGCC TGAGTCTGCT GCACCAGGAG AGCAAGCGCC GGGCCATGCT GGCTGCGGTG 
CTGGAACAGG AGGTGCCCAC ACTAGCAGAG AATCTCCTGG AACAGGAACA GGACTCTCGA 
CTCAGCAAGA TTCATGTGGA ACTTCTGCTT CGGTGCCTTG GGGCACAAAT CCACACTCCT 
AACCGCCGGC AGCTGGCCCA GGAGCTGCGG ACCCTGCAAG CTCAGCTGCG GGCCCAGAGC 
CTGGGCCCTG CGCTTTTGAA GGGGCCGCTC TTCGCCTTTC CAGACGCGGT GAAGCAGATC 
CTCCGCAGAC GCCAGATCCG CCCACACTGG ATGTTCGTGT TGGACTCGCT GCTCAGCCGT 
GCAGTCCGGG CGGCGATGGC GGTGCTGGAC GCGGAGTCGG AGAAGAAAGC GGTCTTACCG 
AGGTCAGAAG AGTCGAGTAA AGAGGAGTCC CAGCAGAAAC CTCAGGAGAG CCAGGCGCTG 
CAGAGCCAGC TCCCACCAGA GCAGGGACCC CCATCGTTGA TGGTGGAGTT GGGCCTTTTG 
CGAGCCGAGA CTGACAGGCT TCGGGACCTT CTGGCTGAGA AGGAACGTGA GTGCCAGGCG 
CTGGTGCAAC AGGCCCTGCA TCGGGTGCAT GCAGAGACCA GGAAGTATGC CCCAGCTTCA 
GAGACCCCAG CCACTCTCCC AAAGGACCAG AACCTGGTGC GGTGGCTACA GGAACTGAGT 
GTAGATCCAG CCACTATCCA AACGCTCCTG AGTCATAGCT TCACCCTTCA AACCCTGCTC 
ACCTGTGCCA CTCAAGATGA TCTAGTGTAC ACCAGAATCA GGGGAGGGAT GGTATGCCGG 
ATTTGGAGAG CCATCTTGGC ACAGCGAGCA GGAGCCACAT CGGTCACCCC AGTACCCCGG 
GACGCTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ASK Family Kinases Are Required for Optimal NLRP3 Inflammasome Priming.
The American journal of pathology(2018 Jan)
Place DE,Samir P,Karki R,Briard B,Vogel P,Kanneganti TD

Changes in gene methylation patterns in neonatal murine hearts: Implications for the regenerative potential.
BMC genomics17231(2016 Mar)
G?rnikiewicz B,Ronowicz A,Krzemi?ski M,Sachadyn P

The ASK family kinases differentially mediate induction of type I interferon and apoptosis during the antiviral response.
Science signaling8(388)ra78(2015 Aug)
Okazaki T,Higuchi M,Takeda K,Iwatsuki-Horimoto K,Kiso M,Miyagishi M,Yanai H,Kato A,Yoneyama M,Fujita T,Taniguchi T,Kawaoka Y,Ichijo H,Gotoh Y

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)
Gineste C,De Winter JM,Kohl C,Witt CC,Giannesini B,Brohm K,Le Fur Y,Gretz N,Vilmen C,Pecchi E,Jubeau M,Cozzone PJ,Stienen GJ,Granzier H,Labeit S,Ottenheijm CA,Bendahan D,Gondin J

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Map3k6 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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