gRNAs
The following Nxf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nxf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu21602 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001276704.1 | |
| Accession Version | NM_001276704.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-20 | |
| Organism | Mus musculus(house mouse) | |
| Product | nuclear RNA export factor 1 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143640.1. Transcript Variant: This variant (2) contains an alternate internal segment and transcription extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK143640.1, SRR1660815.62653.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: translation inferred from conservation ##RefSeq-Attributes-END## | |
1 | ATGGCGGACG AAGGGAAGTC ATACAACGAG CATGATGACC GTGTTAGTTT CCCTCAAAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001263633.1 |
| CDS | 116..1372 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001276704.1 |
1 | ATGGCGGACG AAGGGAAGTC ATACAACGAG CATGATGACC GTGTTAGTTT CCCTCAAAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu10645 | |
| Clone ID Related Accession (Same CDS sequence) | NM_016813.2 | |
| Accession Version | NM_016813.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-20 | |
| Organism | Mus musculus(house mouse) | |
| Product | nuclear RNA export factor 1 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC005594.1. On Jun 19, 2003 this sequence version replaced NM_016813.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005594.1, AK079583.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCGGACG AAGGGAAGTC ATACAACGAG CATGATGACC GTGTTAGTTT CCCTCAAAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_058093.2 |
| CDS | 130..1986 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_016813.2 |
1 | ATGGCGGACG AAGGGAAGTC ATACAACGAG CATGATGACC GTGTTAGTTT CCCTCAAAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Leukemia-Associated Nup214 Fusion Proteins Disturb the XPO1-Mediated Nuclear-Cytoplasmic Transport Pathway and Thereby the NF-?B Signaling Pathway. |
Nxf1 natural variant E610G is a semi-dominant suppressor of IAP-induced RNA processing defects. |
Murine leukemia virus uses NXF1 for nuclear export of spliced and unspliced viral transcripts. |
MLV requires Tap/NXF1-dependent pathway to export its unspliced RNA to the cytoplasm and to express both spliced and unspliced RNAs. |
Generation and characterization of an Nxf7 knockout mouse to study NXF5 deficiency in a patient with intellectual disability. |
