Sgsm1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sgsm1
Entrez Gene ID	52850
Full Name	small G protein signaling modulator 1
Synonyms	2410098H20Rik,AA408781,D5Bwg1524e,E130107M15,NuIP,Rutbc2
General protein information	Preferred Names small G protein signaling modulator 1 Names small G protein signaling modulator 1 RUN and TBC1 domain containing 2 RUN and TBC1 domain-containing protein 2 nurr1-interacting protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 F\|5 55.45 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001254731.1	NP_001241660.1	small G protein signaling modulator 1 isoform c
NM_001162965.1	NP_001156437.1	small G protein signaling modulator 1 isoform b
NM_172718.3	NP_766306.2	small G protein signaling modulator 1 isoform a
NM_001309526.1	NP_001296455.1	small G protein signaling modulator 1 isoform d
XM_006535122.4	XP_006535185.1	small G protein signaling modulator 1 isoform X1
XM_006535122.3	XP_006535185.1	small G protein signaling modulator 1 isoform X1
NM_001309528.1	NP_001296457.1	small G protein signaling modulator 1 isoform e

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sgsm1	NP_001099407.2
Danio rerio (zebrafish)	LOC100535556	XP_003199319.1
Pan troglodytes (chimpanzee)	SGSM1	XP_001171406.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003198	XP_312896.5
Homo sapiens (human)	SGSM1	NP_001035037.1
Bos taurus (cattle)	SGSM1	NP_001179350.1
Danio rerio (zebrafish)	sgsm1	XP_003198667.2
Drosophila melanogaster (fruit fly)	CG1695	NP_728346.2
Drosophila melanogaster (fruit fly)	CG32506	NP_608395.6
	SGSM1	XP_002798359.1
Canis lupus familiaris (dog)	SGSM1	XP_005636411.1
Mus musculus (house mouse)	Sgsm1	NP_766306.2
Gallus gallus (chicken)	SGSM1	XP_003642252.2
Caenorhabditis elegans (roundworm)	tbc-8	NP_001255072.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2.
Zhang Z, Wang S, Shen T, Chen J, Ding J
Structure (London, England : 1993)22(10)1408-20(2014 Oct)

Expression pattern of NuIP gene in adult mouse brain.
Luo Y, Sarabi SA, Backman C, Shan L, Hoffer B, Federoff H
Brain research130242-53(2009 Dec)

Identification of a novel nurr1-interacting protein.
Luo Y, Xing F, Guiliano R, Federoff HJ
The Journal of neuroscience : the official journal of the Society for Neuroscience28(37)9277-86(2008 Sep)

Identification of three novel proteins (SGSM1, 2, 3) which modulate small G protein (RAP and RAB)-mediated signaling pathway.
Yang H, Sasaki T, Minoshima S, Shimizu N
Genomics90(2)249-60(2007 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Crystal structure of the Rab9A-RUTBC2 Rab-binding domain complex reveals that RUTBC2 Rab-binding doamin adopts a PH domain fold and mainly uses beta1 and eta1 insertion to bind Rab9A.
Title: Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2.

Overall, the expression pattern of NuIP in adult mouse brain suggests that it may be involved in motor activity control in basal ganglia as well as higher central nervous system (CNS) functions such as cognition and memory in cortex and hippocampus.
Title: Expression pattern of NuIP gene in adult mouse brain.

The following Sgsm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sgsm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05734	NM_001254731.1 Latest version!	Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu69453	NM_001309526.1 Latest version!	Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu69454	NM_001309528.1 Latest version!	Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu35597	XM_006535122.4 Latest version!	Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu03947	NM_172718.3 Latest version!	Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu08639	NM_001162965.1 Latest version!	Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu35597	XM_006535122.3	Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sgsm1 ( NM_001254731.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001241660.1 Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu05734
Clone ID Related Accession (Same CDS sequence)	NM_001254731.1
Accession Version	NM_001254731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	small G protein signaling modulator 1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC130548.3, AK044716.1 and DV644552.1. On Dec 14, 2011 this sequence version replaced NR_027937.1. Transcript Variant: This variant (5) differs in the 3' UTR and has multiple coding region differences, compared to variant 1, one of which results in a frameshift. The resulting protein (isoform c) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK044716.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCTTCGG TCCCTGCGGA GGCCGAGACC CGCCAGAGGC TGCTGCGCAC TGTGAAGAAG 
GAGGTGAAGC AGATCATGGA AGAAGCCGTT ACCAGAAAGT TCGTCCACGA GGACAGCAGC 
CACATCATCT CCTTCTGTGC GGCCGTGGAG GCCTGCGTGC TGCACGGGCT GCGGCGGAGG 
GCGGCCGGCT TCCTGCGAAG CAACAAGATC GCGGCGCTCT TCATGAAGGT GGGCAAAGGC 
TTCCCTCCGG CCGAGGAGCT GAGCCGCAAG GTCCAGGAAC TGGAGCAACT CATTGAGAGC 
GCGCGAAACC AGATCCAGGG CCTGCAAGAG AACGTGCGGA AGCTGCCGAA GCTGCCTAAC 
CTGTCCCCGC TGGCCATCAA GCACCTGTGG ATCCGCACCG CCCTGTTCGA GAGGGTCCTG 
GACAAAATCG TCCACTACCT GGTGGAAAAC AGCAGTAAAT ACTACGAGAA GGAGGCACTC 
TTGATGGACC CGGTGGACGG ACCCATCCTG GCCTCTTTGT TGGTGGGGCC ATGTGCCCTG 
GAGTATACCA AGATGAAGAC TGCAGATCAC TTCTGGACTG ACCCCTCAGC CGACGAGCTG 
GTCCAGAGAC ACCGGATCCA CAGCTCACAT CTGAGACAGG ACTCGCCCAC CAAGCGGCCA 
GCGCTCTGTA TCCAGAAGAG GCATTCCAGT GGCAGCATGG ACGACCGGCC ATCTATCTCT 
GCCCGGGACT ATGTAGAATC TCTGCACCAG AACTCCAGGG CCACCCTGCT CTATGGAAAG 
AACAATGTTC TGGTTCAGCC AAGGGATGAC ATGGAGGCCG TGCCAGGGTA CCTGTCTCTG 
CACCAGACAG CGGATGTCAT GACCTTGAAG TGGACACCCA ATCAGCTGAT GAACGGGTCT 
GTGGGGGATC TGGACTACGA GAAGAGTGTC TACTGGGACT ACGCTGTGAC CATCCGCTTA 
GAGGAGATAG TTTACCTGCA CTGCCATCAA CAAGTGGACA GCGGCGGGAC TGTCGTGCTG 
GTGAGCCAGG ATGGAATCCA GAGGCCACCC TTCCGCTTCC CCAAGGGCGG GCACCTGCTA 
CAGTTCCTCT CCTGTCTGGA GAATGGGCTG CTTCCGCACG GGCAGCTGGA CCCGCCGCTT 
TGGTCACAGC GAGGAAAGGG GAAGGTATTT CCTAAGTTGC GCAAGCGCAG CCCACAGGGG 
TCCTCCGAGT CCACGTCTTC AGACAAGGAG GACGACGAAG CCACGGATTA CGTGTTCCGC 
ATCATCTACC CTGGCACGCA GTCTGAATTC GTGCCCCAGG ACCTAATGGA TGTCTCTATG 
AACAACCTTC CACCCCTATG GCAACCCAGT CCTCGGAAGT CCTCCTGCTC TTCCTGTTCA 
CAAAGCGGCT CAGCTGACGG CGGCTCAACC AATGGCTGTA ACCATGAGAG GGCCCCACTG 
AAACTGCTGT GTGATAACAT GAAGTACCAG ATCCTCTCCA GAGCCTTCTA TGGATGGCTC 
GCCTACTGCA GACACCTGTC CACCGTGAGG ACCCACCTGT CAGCCCTGGT CAATCACATG 
ATCGTGTCTC CAGACCTGCC CTGTGACGCT GGGCAAGGGT TGACAGCCAG CATCTGGGAA 
AAATACATCC AGGACAGCAC GACCTACCCG GAGCAGGAGC TGCTGCGTCT CATCTACTAT 
GGAGGTGTCC AGCCTGAGAT CCGCAGGGCA GTGTGGCCCT TCCTCCTCGG CCACTACCAG 
TTTGGGATGA CAGAGATGGA GAGGAAAGAG GTAGACGAAC AGATCCATGC CTGCTACGCA 
CAAACCATGT CGGAGTGGCT GGGCTGTGAA GCTATCGTGA GACAGAGGGA GCGGGAGTCC 
CACGCGGCTG CCCTGGCCAA GTGCTCTTCA GGAGCCAGCC TGGACAGCCA CCTTCACCGG 
ATGCTGCACC GGGACTCCAC CATCAGCAAT GAGTCATCCC AGAGCTGCAG CTCAGGCCGC 
CAGAACCTCC GACTGCAGAG CGACTCCAGC AGCAGCACAC AGGTGACTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001241660.1
CDS	95..2128
Translation	MASVPAEAETRQRLLRTVKKEVKQIMEEAVTRKFVHEDSSHIISFCAAVEACVLHGLRRRAAGFLRSNKIAALFMKVGKGFPPAEELSRKVQELEQLIESARNQIQGLQENVRKLPKLPNLSPLAIKHLWIRTALFERVLDKIVHYLVENSSKYYEKEALLMDPVDGPILASLLVGPCALEYTKMKTADHFWTDPSADELVQRHRIHSSHLRQDSPTKRPALCIQKRHSSGSMDDRPSISARDYVESLHQNSRATLLYGKNNVLVQPRDDMEAVPGYLSLHQTADVMTLKWTPNQLMNGSVGDLDYEKSVYWDYAVTIRLEEIVYLHCHQQVDSGGTVVLVSQDGIQRPPFRFPKGGHLLQFLSCLENGLLPHGQLDPPLWSQRGKGKVFPKLRKRSPQGSSESTSSDKEDDEATDYVFRIIYPGTQSEFVPQDLMDVSMNNLPPLWQPSPRKSSCSSCSQSGSADGGSTNGCNHERAPLKLLCDNMKYQILSRAFYGWLAYCRHLSTVRTHLSALVNHMIVSPDLPCDAGQGLTASIWEKYIQDSTTYPEQELLRLIYYGGVQPEIRRAVWPFLLGHYQFGMTEMERKEVDEQIHACYAQTMSEWLGCEAIVRQRERESHAAALAKCSSGASLDSHLHRMLHRDSTISNESSQSCSSGRQNLRLQSDSSSSTQVTF

Target ORF information:

RefSeq Version	NM_001254731.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001254731.1

ORF Insert Sequence:

ATGGCTTCGG TCCCTGCGGA GGCCGAGACC CGCCAGAGGC TGCTGCGCAC TGTGAAGAAG 
GAGGTGAAGC AGATCATGGA AGAAGCCGTT ACCAGAAAGT TCGTCCACGA GGACAGCAGC 
CACATCATCT CCTTCTGTGC GGCCGTGGAG GCCTGCGTGC TGCACGGGCT GCGGCGGAGG 
GCGGCCGGCT TCCTGCGAAG CAACAAGATC GCGGCGCTCT TCATGAAGGT GGGCAAAGGC 
TTCCCTCCGG CCGAGGAGCT GAGCCGCAAG GTCCAGGAAC TGGAGCAACT CATTGAGAGC 
GCGCGAAACC AGATCCAGGG CCTGCAAGAG AACGTGCGGA AGCTGCCGAA GCTGCCTAAC 
CTGTCCCCGC TGGCCATCAA GCACCTGTGG ATCCGCACCG CCCTGTTCGA GAGGGTCCTG 
GACAAAATCG TCCACTACCT GGTGGAAAAC AGCAGTAAAT ACTACGAGAA GGAGGCACTC 
TTGATGGACC CGGTGGACGG ACCCATCCTG GCCTCTTTGT TGGTGGGGCC ATGTGCCCTG 
GAGTATACCA AGATGAAGAC TGCAGATCAC TTCTGGACTG ACCCCTCAGC CGACGAGCTG 
GTCCAGAGAC ACCGGATCCA CAGCTCACAT CTGAGACAGG ACTCGCCCAC CAAGCGGCCA 
GCGCTCTGTA TCCAGAAGAG GCATTCCAGT GGCAGCATGG ACGACCGGCC ATCTATCTCT 
GCCCGGGACT ATGTAGAATC TCTGCACCAG AACTCCAGGG CCACCCTGCT CTATGGAAAG 
AACAATGTTC TGGTTCAGCC AAGGGATGAC ATGGAGGCCG TGCCAGGGTA CCTGTCTCTG 
CACCAGACAG CGGATGTCAT GACCTTGAAG TGGACACCCA ATCAGCTGAT GAACGGGTCT 
GTGGGGGATC TGGACTACGA GAAGAGTGTC TACTGGGACT ACGCTGTGAC CATCCGCTTA 
GAGGAGATAG TTTACCTGCA CTGCCATCAA CAAGTGGACA GCGGCGGGAC TGTCGTGCTG 
GTGAGCCAGG ATGGAATCCA GAGGCCACCC TTCCGCTTCC CCAAGGGCGG GCACCTGCTA 
CAGTTCCTCT CCTGTCTGGA GAATGGGCTG CTTCCGCACG GGCAGCTGGA CCCGCCGCTT 
TGGTCACAGC GAGGAAAGGG GAAGGTATTT CCTAAGTTGC GCAAGCGCAG CCCACAGGGG 
TCCTCCGAGT CCACGTCTTC AGACAAGGAG GACGACGAAG CCACGGATTA CGTGTTCCGC 
ATCATCTACC CTGGCACGCA GTCTGAATTC GTGCCCCAGG ACCTAATGGA TGTCTCTATG 
AACAACCTTC CACCCCTATG GCAACCCAGT CCTCGGAAGT CCTCCTGCTC TTCCTGTTCA 
CAAAGCGGCT CAGCTGACGG CGGCTCAACC AATGGCTGTA ACCATGAGAG GGCCCCACTG 
AAACTGCTGT GTGATAACAT GAAGTACCAG ATCCTCTCCA GAGCCTTCTA TGGATGGCTC 
GCCTACTGCA GACACCTGTC CACCGTGAGG ACCCACCTGT CAGCCCTGGT CAATCACATG 
ATCGTGTCTC CAGACCTGCC CTGTGACGCT GGGCAAGGGT TGACAGCCAG CATCTGGGAA 
AAATACATCC AGGACAGCAC GACCTACCCG GAGCAGGAGC TGCTGCGTCT CATCTACTAT 
GGAGGTGTCC AGCCTGAGAT CCGCAGGGCA GTGTGGCCCT TCCTCCTCGG CCACTACCAG 
TTTGGGATGA CAGAGATGGA GAGGAAAGAG GTAGACGAAC AGATCCATGC CTGCTACGCA 
CAAACCATGT CGGAGTGGCT GGGCTGTGAA GCTATCGTGA GACAGAGGGA GCGGGAGTCC 
CACGCGGCTG CCCTGGCCAA GTGCTCTTCA GGAGCCAGCC TGGACAGCCA CCTTCACCGG 
ATGCTGCACC GGGACTCCAC CATCAGCAAT GAGTCATCCC AGAGCTGCAG CTCAGGCCGC 
CAGAACCTCC GACTGCAGAG CGACTCCAGC AGCAGCACAC AGGTGACTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sgsm1 ( NM_001309526.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001296455.1 Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu69453
Clone ID Related Accession (Same CDS sequence)	NM_001309526.1
Accession Version	NM_001309526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	small G protein signaling modulator 1 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK051018.1 and AC151577.2. On Jun 9, 2015 this sequence version replaced NR_027936.1. Transcript Variant: This variant (4) differs in both UTRs, lacks a portion of the 5' and 3' coding regions, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (d) is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: AK051018.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCTGGGAC AAGTAGGTCT TGGTGACCTC TGGCTTCCCA CAGTCTCCCT CACTTCCCCT 
TCCAGTGTCT ACTGGGACTA CGCTGTGACC ATCCGCTTAG AGGAGATAGT TTACCTGCAC 
TGCCATCAAC AAGTGGACAG CGGCGGGACT GTCGTGCTGG TGAGCCAGGA TGGAATCCAG 
AGGCCACCCT TCCGCTTCCC CAAGGGCGGG CACCTGCTAC AGTTCCTCTC CTGTCTGGAG 
AATGGGCTGC TTCCGCACGG GCAGCTGGAC CCGCCGCTTT GGTCACAGCG AGGAAAGGGG 
AAGGTATTTC CTAAGTTGCG CAAGCGCAGC CCACAGGGGT CCTCCGAGTC CACGTCTTCA 
GACAAGGAGG ACGACGAAGC CACGGATTAC GTGTTCCGCA TCATCTACCC TGGCACGCAG 
TCTGAATTCG TGCCCCAGGA CCTAATGGAT GTCTCTATGA ACAACCTTCC ACCCCTATGG 
CAACCCAGTC CTCGGAAGTC CTCCTGCTCT TCCTGTTCAC AAAGCGGCTC AGCTGACGGC 
GGCTCAACCA ATGGCTGTAA CCATGAGAGG GCCCCACTGA AACTGCTGTG TGATAACATG 
AAGTACCAGA TCCTCTCCAG AGCCTTCTAT GGATGGCTCG CCTACTGCAG ACACCTGTCC 
ACCGTGAGGA CCCACCTGTC AGCCCTGGTC AATCACATGA TCGTGTCTCC AGACCTGCCC 
TGTGACGCTG GGCAAGGGTT GACAGCCAGC ATCTGGGAAA AATACATCCA GGACAGCACG 
ACCTACCCGG AGCAGGAGCT GCTGCGTCTC ATCTACTATG GAGGTGTCCA GCCTGAGATC 
CGCAGGGCAG TGTGGCCCTT CCTCCTCGGC CACTACCAGT TTGGGATGAC AGAGATGGAG 
AGGAAAGAGG TAGACGAACA GATCCATGCC TGCTACGCAC AAACCATGTC GGAGTGGCTG 
GGCTGTGAAG CTATCGTGAG ACAGAGGGAG CGGGAGTCCC ACGCGGCTGC CCTGGCCAAG 
TGCTCTTCAG GAGCCAGCCT GGACAGCCAC CTTCACCGGA TGCTGCACCG GGACTCCACC 
ATCAGCAATG AGTCATCCCA GAGCTGCAGC TCAGGCCGCC AGAACCTCCG ACTGCAGAGC 
GACTCCAGCA GCAGCACACA GGTATTTGAG TCTGTGGATG AGGTGGAGCA GACAGAGGCA 
GAAGGCAGGT CAGAAGAAAA ACATCCCAAA ATCCCCAATG GGAACCCAGC CAACGGCACT 
TGCTCCCCAG ACTCCGGACA CCCTTCCTCC CACAACTTCT CCTCTGGTCT CTCCGAGCAC 
TCTGAGCCCA GTCTTAGCAC TGAAGACAGC GTCTTGGATG CCCAACGCAG CCTTCCAGCT 
GTGTTTCGGC CTGGGGACAG CAGCGTGGAA GATGGGCAGA GCAGCGAAGC CACCACATCC 
CGGGACGAGG CCCCTCGGGA GGAGCTGGCC GTGCAAGATA GCCTTGAGAG TGACCTCCTT 
GCCAACGAGA GCTTGGAGGA GTTCATGTCT ATCCCTGGCA GCCTGGACGT GGCCCTACCT 
GAGAAGGATG GCGCGGTGAT GGACGGCTGG CCCGGTGAGG CGGACAAGCC CAGTCGGGCT 
GACAGTGAGG ACAACCTCTC AGAAGAGCCT GAGATGGAGA GCCTGTTCCC TGCCCTGGCT 
TCTCTGGCTG TGACCTCCTC TGCCAATAAC GAGGCATCCC CTGTGTCCTC CAGTGGAGTC 
ACCTATTCTC CAGAACTACT GGACCTGTAC ACCGTGAACC TGCACCGCAT TGAGAAAGAT 
GTGCAGAGGT GTGACCGCAG CTACTGGTAC TTCACAGCAG CCAACCTGGA GAAGCTGAGG 
AACATCATGT GCAGCTATAT CTGGCAGCAC ATCGAGATCG GCTACGTCCA GGGCATGTGT 
GACCTCCTGG CCCCGTTGCT GGTCATCCTG GATGATGACC AGGTTTCTCT GTATAGCCCC 
AGCTGTCCTG GAACTCAAGT TGTAGACCAG GCTGGCCTCG AACTCAGAAA TCCGCCTGCC 
TTTGCCTTCC CAATGCTGGG ATTAGAGGCA TGCGCCACCA CCGCCCGGCT AGATAATTGG 
AATGTTAGCA GCTCACTTGG CGATACCAGT CCTCACCGGC ATCTCACATT TACCCTGATG 
TCTACGTCAG ACAGAATCAT TCCAGGGAAA TGTCAGACTA GTACCTCTCA TGAATATAAA 
AGCAAAACGT CTCAAAAAAA AATCAACAAA CTCAATCCAA GAAGATATAA AATATTACAC 
TGCATGGCCA AGCGGGTCTT ATCCCAGAAA CGCAAGGGTG GTTCAATATC CAAGCATCAA 
TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296455.1
CDS	143..2491
Translation	MLGQVGLGDLWLPTVSLTSPSSVYWDYAVTIRLEEIVYLHCHQQVDSGGTVVLVSQDGIQRPPFRFPKGGHLLQFLSCLENGLLPHGQLDPPLWSQRGKGKVFPKLRKRSPQGSSESTSSDKEDDEATDYVFRIIYPGTQSEFVPQDLMDVSMNNLPPLWQPSPRKSSCSSCSQSGSADGGSTNGCNHERAPLKLLCDNMKYQILSRAFYGWLAYCRHLSTVRTHLSALVNHMIVSPDLPCDAGQGLTASIWEKYIQDSTTYPEQELLRLIYYGGVQPEIRRAVWPFLLGHYQFGMTEMERKEVDEQIHACYAQTMSEWLGCEAIVRQRERESHAAALAKCSSGASLDSHLHRMLHRDSTISNESSQSCSSGRQNLRLQSDSSSSTQVFESVDEVEQTEAEGRSEEKHPKIPNGNPANGTCSPDSGHPSSHNFSSGLSEHSEPSLSTEDSVLDAQRSLPAVFRPGDSSVEDGQSSEATTSRDEAPREELAVQDSLESDLLANESLEEFMSIPGSLDVALPEKDGAVMDGWPGEADKPSRADSEDNLSEEPEMESLFPALASLAVTSSANNEASPVSSSGVTYSPELLDLYTVNLHRIEKDVQRCDRSYWYFTAANLEKLRNIMCSYIWQHIEIGYVQGMCDLLAPLLVILDDDQVSLYSPSCPGTQVVDQAGLELRNPPAFAFPMLGLEACATTARLDNWNVSSSLGDTSPHRHLTFTLMSTSDRIIPGKCQTSTSHEYKSKTSQKKINKLNPRRYKILHCMAKRVLSQKRKGGSISKHQSV

Target ORF information:

RefSeq Version	NM_001309526.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001309526.1

ORF Insert Sequence:

ATGCTGGGAC AAGTAGGTCT TGGTGACCTC TGGCTTCCCA CAGTCTCCCT CACTTCCCCT 
TCCAGTGTCT ACTGGGACTA CGCTGTGACC ATCCGCTTAG AGGAGATAGT TTACCTGCAC 
TGCCATCAAC AAGTGGACAG CGGCGGGACT GTCGTGCTGG TGAGCCAGGA TGGAATCCAG 
AGGCCACCCT TCCGCTTCCC CAAGGGCGGG CACCTGCTAC AGTTCCTCTC CTGTCTGGAG 
AATGGGCTGC TTCCGCACGG GCAGCTGGAC CCGCCGCTTT GGTCACAGCG AGGAAAGGGG 
AAGGTATTTC CTAAGTTGCG CAAGCGCAGC CCACAGGGGT CCTCCGAGTC CACGTCTTCA 
GACAAGGAGG ACGACGAAGC CACGGATTAC GTGTTCCGCA TCATCTACCC TGGCACGCAG 
TCTGAATTCG TGCCCCAGGA CCTAATGGAT GTCTCTATGA ACAACCTTCC ACCCCTATGG 
CAACCCAGTC CTCGGAAGTC CTCCTGCTCT TCCTGTTCAC AAAGCGGCTC AGCTGACGGC 
GGCTCAACCA ATGGCTGTAA CCATGAGAGG GCCCCACTGA AACTGCTGTG TGATAACATG 
AAGTACCAGA TCCTCTCCAG AGCCTTCTAT GGATGGCTCG CCTACTGCAG ACACCTGTCC 
ACCGTGAGGA CCCACCTGTC AGCCCTGGTC AATCACATGA TCGTGTCTCC AGACCTGCCC 
TGTGACGCTG GGCAAGGGTT GACAGCCAGC ATCTGGGAAA AATACATCCA GGACAGCACG 
ACCTACCCGG AGCAGGAGCT GCTGCGTCTC ATCTACTATG GAGGTGTCCA GCCTGAGATC 
CGCAGGGCAG TGTGGCCCTT CCTCCTCGGC CACTACCAGT TTGGGATGAC AGAGATGGAG 
AGGAAAGAGG TAGACGAACA GATCCATGCC TGCTACGCAC AAACCATGTC GGAGTGGCTG 
GGCTGTGAAG CTATCGTGAG ACAGAGGGAG CGGGAGTCCC ACGCGGCTGC CCTGGCCAAG 
TGCTCTTCAG GAGCCAGCCT GGACAGCCAC CTTCACCGGA TGCTGCACCG GGACTCCACC 
ATCAGCAATG AGTCATCCCA GAGCTGCAGC TCAGGCCGCC AGAACCTCCG ACTGCAGAGC 
GACTCCAGCA GCAGCACACA GGTATTTGAG TCTGTGGATG AGGTGGAGCA GACAGAGGCA 
GAAGGCAGGT CAGAAGAAAA ACATCCCAAA ATCCCCAATG GGAACCCAGC CAACGGCACT 
TGCTCCCCAG ACTCCGGACA CCCTTCCTCC CACAACTTCT CCTCTGGTCT CTCCGAGCAC 
TCTGAGCCCA GTCTTAGCAC TGAAGACAGC GTCTTGGATG CCCAACGCAG CCTTCCAGCT 
GTGTTTCGGC CTGGGGACAG CAGCGTGGAA GATGGGCAGA GCAGCGAAGC CACCACATCC 
CGGGACGAGG CCCCTCGGGA GGAGCTGGCC GTGCAAGATA GCCTTGAGAG TGACCTCCTT 
GCCAACGAGA GCTTGGAGGA GTTCATGTCT ATCCCTGGCA GCCTGGACGT GGCCCTACCT 
GAGAAGGATG GCGCGGTGAT GGACGGCTGG CCCGGTGAGG CGGACAAGCC CAGTCGGGCT 
GACAGTGAGG ACAACCTCTC AGAAGAGCCT GAGATGGAGA GCCTGTTCCC TGCCCTGGCT 
TCTCTGGCTG TGACCTCCTC TGCCAATAAC GAGGCATCCC CTGTGTCCTC CAGTGGAGTC 
ACCTATTCTC CAGAACTACT GGACCTGTAC ACCGTGAACC TGCACCGCAT TGAGAAAGAT 
GTGCAGAGGT GTGACCGCAG CTACTGGTAC TTCACAGCAG CCAACCTGGA GAAGCTGAGG 
AACATCATGT GCAGCTATAT CTGGCAGCAC ATCGAGATCG GCTACGTCCA GGGCATGTGT 
GACCTCCTGG CCCCGTTGCT GGTCATCCTG GATGATGACC AGGTTTCTCT GTATAGCCCC 
AGCTGTCCTG GAACTCAAGT TGTAGACCAG GCTGGCCTCG AACTCAGAAA TCCGCCTGCC 
TTTGCCTTCC CAATGCTGGG ATTAGAGGCA TGCGCCACCA CCGCCCGGCT AGATAATTGG 
AATGTTAGCA GCTCACTTGG CGATACCAGT CCTCACCGGC ATCTCACATT TACCCTGATG 
TCTACGTCAG ACAGAATCAT TCCAGGGAAA TGTCAGACTA GTACCTCTCA TGAATATAAA 
AGCAAAACGT CTCAAAAAAA AATCAACAAA CTCAATCCAA GAAGATATAA AATATTACAC 
TGCATGGCCA AGCGGGTCTT ATCCCAGAAA CGCAAGGGTG GTTCAATATC CAAGCATCAA 
TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sgsm1 ( NM_001309528.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001296457.1 Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 2, mRNA.

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CloneID	OMu69454
Clone ID Related Accession (Same CDS sequence)	NM_001309528.1
Accession Version	NM_001309528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	small G protein signaling modulator 1 isoform e
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK051018.1, AK053555.1, AK167632.1 and BP761545.1. On Jun 9, 2015 this sequence version replaced NR_027934.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (e) has a shorter N-terminus than isoform a. ##Evidence-Data-START## Transcript exon combination :: AK167632.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCTGGGAC AAGTAGGTCT TGGTGACCTC TGGCTTCCCA CAGTCTCCCT CACTTCCCCT 
TCCAGTGTCT ACTGGGACTA CGCTGTGACC ATCCGCTTAG AGGAGATAGT TTACCTGCAC 
TGCCATCAAC AAGTGGACAG CGGCGGGACT GTCGTGCTGG TGAGCCAGGA TGGAATCCAG 
AGGCCACCCT TCCGCTTCCC CAAGGGCGGG CACCTGCTAC AGTTCCTCTC CTGTCTGGAG 
AATGGGCTGC TTCCGCACGG GCAGCTGGAC CCGCCGCTTT GGTCACAGCG AGGAAAGGGG 
AAGGTATTTC CTAAGTTGCG CAAGCGCAGC CCACAGGGGT CCTCCGAGTC CACGTCTTCA 
GACAAGGAGG ACGACGAAGC CACGGATTAC GTGTTCCGCA TCATCTACCC TGGCACGCAG 
TCTGAATTCG TGCCCCAGGA CCTAATGGAT GTCTCTATGA ACAACCTTCC ACCCCTATGG 
CAACCCAGTC CTCGGAAGTC CTCCTGCTCT TCCTGTTCAC AAAGCGGCTC AGCTGACGGC 
GGCTCAACCA ATGGCTGTAA CCATGAGAGG GCCCCACTGA AACTGCTGTG TGATAACATG 
AAGTACCAGA TCCTCTCCAG AGCCTTCTAT GGATGGCTCG CCTACTGCAG ACACCTGTCC 
ACCGTGAGGA CCCACCTGTC AGCCCTGGTC AATCACATGA TCGTGTCTCC AGACCTGCCC 
TGTGACGCTG GGCAAGGGTT GACAGCCAGC ATCTGGGAAA AATACATCCA GGACAGCACG 
ACCTACCCGG AGCAGGAGCT GCTGCGTCTC ATCTACTATG GAGGTGTCCA GCCTGAGATC 
CGCAGGGCAG TGTGGCCCTT CCTCCTCGGC CACTACCAGT TTGGGATGAC AGAGATGGAG 
AGGAAAGAGG TAGACGAACA GATCCATGCC TGCTACGCAC AAACCATGTC GGAGTGGCTG 
GGCTGTGAAG CTATCGTGAG ACAGAGGGAG CGGGAGTCCC ACGCGGCTGC CCTGGCCAAG 
TGCTCTTCAG GAGCCAGCCT GGACAGCCAC CTTCACCGGA TGCTGCACCG GGACTCCACC 
ATCAGCAATG AGTCATCCCA GAGCTGCAGC TCAGGCCGCC AGAACCTCCG ACTGCAGAGC 
GACTCCAGCA GCAGCACACA GGTATTTGAG TCTGTGGATG AGGTGGAGCA GACAGAGGCA 
GAAGGCAGGT CAGAAGAAAA ACATCCCAAA ATCCCCAATG GGAACCCAGC CAACGGCACT 
TGCTCCCCAG ACTCCGGACA CCCTTCCTCC CACAACTTCT CCTCTGGTCT CTCCGAGCAC 
TCTGAGCCCA GTCTTAGCAC TGAAGACAGC GTCTTGGATG CCCAACGCAG CCTTCCAGCT 
GTGTTTCGGC CTGGGGACAG CAGCGTGGAA GATGGGCAGA GCAGCGAAGC CACCACATCC 
CGGGACGAGG CCCCTCGGGA GGAGCTGGCC GTGCAAGATA GCCTTGAGAG TGACCTCCTT 
GCCAACGAGA GCTTGGAGGA GTTCATGTCT ATCCCTGGCA GCCTGGACGT GGCCCTACCT 
GAGAAGGATG GCGCGGTGAT GGACGGCTGG CCCGGTGAGG CGGACAAGCC CAGTCGGGCT 
GACAGTGAGG ACAACCTCTC AGAAGAGCCT GAGATGGAGA GCCTGTTCCC TGCCCTGGCT 
TCTCTGGCTG TGACCTCCTC TGCCAATAAC GAGGCATCCC CTGTGTCCTC CAGTGGAGTC 
ACCTATTCTC CAGAACTACT GGACCTGTAC ACCGTGAACC TGCACCGCAT TGAGAAAGAT 
GTGCAGAGGT GTGACCGCAG CTACTGGTAC TTCACAGCAG CCAACCTGGA GAAGCTGAGG 
AACATCATGT GCAGCTATAT CTGGCAGCAC ATCGAGATCG GCTACGTCCA GGGCATGTGT 
GACCTCCTGG CCCCGTTGCT GGTCATCCTG GATGATGAGG CCCTGGCCTT CAGTTGCTTC 
ACGGAGCTGA TGAAGAGGAT GAACCAAAAC TTCCCCCATG GAGGCGCCAT GGATACGCAC 
TTCGCCAACA TGAGGTCTCT GATCCAGATC CTGGACTCCG AGCTCTTCGA GCTGATGCAT 
CAGAACGGCG ATTACACCCA CTTCTACTTT TGCTACCGCT GGTTCCTGCT GGATTTCAAA 
CGAGAGCTGG TCTATGATGA CGTCTTCTCC GTGTGGGAAA CTATCTGGGC TGCTAAGCAC 
GTGTCCTCCG CCCACTACGT GCTGTTCATC GCGCTGGCGC TGGTGGAGGT CTACAGAGAC 
ATCATCCTGG AGAACAATAT GGACTTCACT GACATTATCA AGTTCTTCAA CGAAATGGCT 
GAGCGGCACA ACGCCAAGCA GATTCTGCAG CTCGCCCGGG ACCTCGTGCA TAAGGTGCAG 
ATTCTAATTG AGAACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296457.1
CDS	143..2563
Translation	MLGQVGLGDLWLPTVSLTSPSSVYWDYAVTIRLEEIVYLHCHQQVDSGGTVVLVSQDGIQRPPFRFPKGGHLLQFLSCLENGLLPHGQLDPPLWSQRGKGKVFPKLRKRSPQGSSESTSSDKEDDEATDYVFRIIYPGTQSEFVPQDLMDVSMNNLPPLWQPSPRKSSCSSCSQSGSADGGSTNGCNHERAPLKLLCDNMKYQILSRAFYGWLAYCRHLSTVRTHLSALVNHMIVSPDLPCDAGQGLTASIWEKYIQDSTTYPEQELLRLIYYGGVQPEIRRAVWPFLLGHYQFGMTEMERKEVDEQIHACYAQTMSEWLGCEAIVRQRERESHAAALAKCSSGASLDSHLHRMLHRDSTISNESSQSCSSGRQNLRLQSDSSSSTQVFESVDEVEQTEAEGRSEEKHPKIPNGNPANGTCSPDSGHPSSHNFSSGLSEHSEPSLSTEDSVLDAQRSLPAVFRPGDSSVEDGQSSEATTSRDEAPREELAVQDSLESDLLANESLEEFMSIPGSLDVALPEKDGAVMDGWPGEADKPSRADSEDNLSEEPEMESLFPALASLAVTSSANNEASPVSSSGVTYSPELLDLYTVNLHRIEKDVQRCDRSYWYFTAANLEKLRNIMCSYIWQHIEIGYVQGMCDLLAPLLVILDDEALAFSCFTELMKRMNQNFPHGGAMDTHFANMRSLIQILDSELFELMHQNGDYTHFYFCYRWFLLDFKRELVYDDVFSVWETIWAAKHVSSAHYVLFIALALVEVYRDIILENNMDFTDIIKFFNEMAERHNAKQILQLARDLVHKVQILIENK

Target ORF information:

RefSeq Version	NM_001309528.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001309528.1

ORF Insert Sequence:

ATGCTGGGAC AAGTAGGTCT TGGTGACCTC TGGCTTCCCA CAGTCTCCCT CACTTCCCCT 
TCCAGTGTCT ACTGGGACTA CGCTGTGACC ATCCGCTTAG AGGAGATAGT TTACCTGCAC 
TGCCATCAAC AAGTGGACAG CGGCGGGACT GTCGTGCTGG TGAGCCAGGA TGGAATCCAG 
AGGCCACCCT TCCGCTTCCC CAAGGGCGGG CACCTGCTAC AGTTCCTCTC CTGTCTGGAG 
AATGGGCTGC TTCCGCACGG GCAGCTGGAC CCGCCGCTTT GGTCACAGCG AGGAAAGGGG 
AAGGTATTTC CTAAGTTGCG CAAGCGCAGC CCACAGGGGT CCTCCGAGTC CACGTCTTCA 
GACAAGGAGG ACGACGAAGC CACGGATTAC GTGTTCCGCA TCATCTACCC TGGCACGCAG 
TCTGAATTCG TGCCCCAGGA CCTAATGGAT GTCTCTATGA ACAACCTTCC ACCCCTATGG 
CAACCCAGTC CTCGGAAGTC CTCCTGCTCT TCCTGTTCAC AAAGCGGCTC AGCTGACGGC 
GGCTCAACCA ATGGCTGTAA CCATGAGAGG GCCCCACTGA AACTGCTGTG TGATAACATG 
AAGTACCAGA TCCTCTCCAG AGCCTTCTAT GGATGGCTCG CCTACTGCAG ACACCTGTCC 
ACCGTGAGGA CCCACCTGTC AGCCCTGGTC AATCACATGA TCGTGTCTCC AGACCTGCCC 
TGTGACGCTG GGCAAGGGTT GACAGCCAGC ATCTGGGAAA AATACATCCA GGACAGCACG 
ACCTACCCGG AGCAGGAGCT GCTGCGTCTC ATCTACTATG GAGGTGTCCA GCCTGAGATC 
CGCAGGGCAG TGTGGCCCTT CCTCCTCGGC CACTACCAGT TTGGGATGAC AGAGATGGAG 
AGGAAAGAGG TAGACGAACA GATCCATGCC TGCTACGCAC AAACCATGTC GGAGTGGCTG 
GGCTGTGAAG CTATCGTGAG ACAGAGGGAG CGGGAGTCCC ACGCGGCTGC CCTGGCCAAG 
TGCTCTTCAG GAGCCAGCCT GGACAGCCAC CTTCACCGGA TGCTGCACCG GGACTCCACC 
ATCAGCAATG AGTCATCCCA GAGCTGCAGC TCAGGCCGCC AGAACCTCCG ACTGCAGAGC 
GACTCCAGCA GCAGCACACA GGTATTTGAG TCTGTGGATG AGGTGGAGCA GACAGAGGCA 
GAAGGCAGGT CAGAAGAAAA ACATCCCAAA ATCCCCAATG GGAACCCAGC CAACGGCACT 
TGCTCCCCAG ACTCCGGACA CCCTTCCTCC CACAACTTCT CCTCTGGTCT CTCCGAGCAC 
TCTGAGCCCA GTCTTAGCAC TGAAGACAGC GTCTTGGATG CCCAACGCAG CCTTCCAGCT 
GTGTTTCGGC CTGGGGACAG CAGCGTGGAA GATGGGCAGA GCAGCGAAGC CACCACATCC 
CGGGACGAGG CCCCTCGGGA GGAGCTGGCC GTGCAAGATA GCCTTGAGAG TGACCTCCTT 
GCCAACGAGA GCTTGGAGGA GTTCATGTCT ATCCCTGGCA GCCTGGACGT GGCCCTACCT 
GAGAAGGATG GCGCGGTGAT GGACGGCTGG CCCGGTGAGG CGGACAAGCC CAGTCGGGCT 
GACAGTGAGG ACAACCTCTC AGAAGAGCCT GAGATGGAGA GCCTGTTCCC TGCCCTGGCT 
TCTCTGGCTG TGACCTCCTC TGCCAATAAC GAGGCATCCC CTGTGTCCTC CAGTGGAGTC 
ACCTATTCTC CAGAACTACT GGACCTGTAC ACCGTGAACC TGCACCGCAT TGAGAAAGAT 
GTGCAGAGGT GTGACCGCAG CTACTGGTAC TTCACAGCAG CCAACCTGGA GAAGCTGAGG 
AACATCATGT GCAGCTATAT CTGGCAGCAC ATCGAGATCG GCTACGTCCA GGGCATGTGT 
GACCTCCTGG CCCCGTTGCT GGTCATCCTG GATGATGAGG CCCTGGCCTT CAGTTGCTTC 
ACGGAGCTGA TGAAGAGGAT GAACCAAAAC TTCCCCCATG GAGGCGCCAT GGATACGCAC 
TTCGCCAACA TGAGGTCTCT GATCCAGATC CTGGACTCCG AGCTCTTCGA GCTGATGCAT 
CAGAACGGCG ATTACACCCA CTTCTACTTT TGCTACCGCT GGTTCCTGCT GGATTTCAAA 
CGAGAGCTGG TCTATGATGA CGTCTTCTCC GTGTGGGAAA CTATCTGGGC TGCTAAGCAC 
GTGTCCTCCG CCCACTACGT GCTGTTCATC GCGCTGGCGC TGGTGGAGGT CTACAGAGAC 
ATCATCCTGG AGAACAATAT GGACTTCACT GACATTATCA AGTTCTTCAA CGAAATGGCT 
GAGCGGCACA ACGCCAAGCA GATTCTGCAG CTCGCCCGGG ACCTCGTGCA TAAGGTGCAG 
ATTCTAATTG AGAACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sgsm1 ( XM_006535122.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535185.1 Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant X1, mRNA.

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CloneID	OMu35597
Clone ID Related Accession (Same CDS sequence)	XM_006535122.4 , XM_006535122.3
Accession Version	XM_006535122.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	small G protein signaling modulator 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006535122.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG TCCCTGCGGA GGCCGAGACC CGCCAGAGGC TGCTGCGCAC TGTGAAGAAG 
GAGGTGAAGC AGATCATGGA AGAAGCCGTT ACCAGAAAGT TCGTCCACGA GGACAGCAGC 
CACATCATCT CCTTCTGTGC GGCCGTGGAG GCCTGCGTGC TGCACGGGCT GCGGCGGAGG 
GCGGCCGGCT TCCTGCGAAG CAACAAGATC GCGGCGCTCT TCATGAAGGT GGGCAAAGGC 
TTCCCTCCGG CCGAGGAGCT GAGCCGCAAG GTCCAGGAAC TGGAGCAACT CATTGAGAGC 
GCGCGAAACC AGATCCAGGG CCTGCAAGAG AACGTGCGGA AGCTGCCGAA GCTGCCTAAC 
CTGTCCCCGC TGGCCATCAA GCACCTGTGG ATCCGCACCG CCCTGTTCGA GAGGGTCCTG 
GACAAAATCG TCCACTACCT GGTGGAAAAC AGCAGTAAAT ACTACGAGAA GGAGGCACTC 
TTGATGGACC CGGTGGACGG ACCCATCCTG GCCTCTTTGT TGGTGGGGCC ATGTGCCCTG 
GAGTATACCA AGATGAAGAC TGCAGATCAC TTCTGGACTG ACCCCTCAGC CGACGAGCTG 
GTCCAGAGAC ACCGGATCCA CAGCTCACAT CTGAGACAGG ACTCGCCCAC CAAGCGGCCA 
GCGCTCTGTA TCCAGAAGAG GCATTCCAGT GGCAGCATGG ACGACCGGCC ATCTATCTCT 
GCCCGGGACT ATGTAGAATC TCTGCACCAG AACTCCAGGG CCACCCTGCT CTATGGAAAG 
AACAATGTTC TGGTTCAGCC AAGGGATGAC ATGGAGGCCG TGCCAGGGTA CCTGTCTCTG 
CACCAGACAG CGGATGTCAT GACCTTGAAG TGGACACCCA ATCAGCTGAT GAACGGGTCT 
GTGGGGGATC TGGACTACGA GAAGAGTGTC TACTGGGACT ACGCTGTGAC CATCCGCTTA 
GAGGAGATAG TTTACCTGCA CTGCCATCAA CAAGTGGACA GCGGCGGGAC TGTCGTGCTG 
GTGAGCCAGG ATGGAATCCA GAGGCCACCC TTCCGCTTCC CCAAGGGCGG GCACCTGCTA 
CAGTTCCTCT CCTGTCTGGA GAATGGGCTG CTTCCGCACG GGCAGCTGGA CCCGCCGCTT 
TGGTCACAGC GAGGAAAGGG GAAGGTATTT CCTAAGTTGC GCAAGCGCAG CCCACAGGGG 
TCCTCCGAGT CCACGTCTTC AGACAAGGAG GACGACGAAG CCACGGATTA CGTGTTCCGC 
ATCATCTACC CTGGCACGCA GTCTGAATTC GTTGCCCTTG ACCTCATGGG CAGCCCCCGG 
CCTGTGTCCA TTGGCCCTGC CTGGATGATG CTTGCTGCGG GCCAGTCCAT GCTAGTGGTG 
GCCAAGGGGC TCCAGTGGGC ACAAACCAGA GGGTGCCTCA CCATCCCCAC AAGGAGTGTG 
AAGGAGCAGC CCCCCATGCC CCAGGACCTA ATGGATGTCT CTATGAACAA CCTTCCACCC 
CTATGGCAAC CCAGTCCTCG GAAGTCCTCC TGCTCTTCCT GTTCACAAAG CGGCTCAGCT 
GACGGCGGCT CAACCAATGG CTGTAACCAT GAGAGGGCCC CACTGAAACT GCTGTGTGAT 
AACATGAAGT ACCAGATCCT CTCCAGAGCC TTCTATGGAT GGCTCGCCTA CTGCAGACAC 
CTGTCCACCG TGAGGACCCA CCTGTCAGCC CTGGTCAATC ACATGATCGT GTCTCCAGAC 
CTGCCCTGTG ACGCTGGGCA AGGGTTGACA GCCAGCATCT GGGAAAAATA CATCCAGGAC 
AGCACGACCT ACCCGGAGCA GGAGCTGCTG CGTCTCATCT ACTATGGAGG TGTCCAGCCT 
GAGATCCGCA GGGCAGTGTG GCCCTTCCTC CTCGGCCACT ACCAGTTTGG GATGACAGAG 
ATGGAGAGGA AAGAGGTAGA CGAACAGATC CATGCCTGCT ACGCACAAAC CATGTCGGAG 
TGGCTGGGCT GTGAAGCTAT CGTGAGACAG AGGGAGCGGG AGTCCCACGC GGCTGCCCTG 
GCCAAGTGCT CTTCAGGAGC CAGCCTGGAC AGCCACCTTC ACCGGATGCT GCACCGGGAC 
TCCACCATCA GCAATGAGTC ATCCCAGAGC TGCAGCTCAG GCCGCCAGAA CCTCCGACTG 
CAGAGCGACT CCAGCAGCAG CACACAGGTA TTTGAGTCTG TGGATGAGGT GGAGCAGACA 
GAGGCAGAAG GCAGGTCAGA AGAAAAACAT CCCAAAATCC CCAATGGGAA CCCAGCCAAC 
GGCACTTGCT CCCCAGACTC CGGACACCCT TCCTCCCACA ACTTCTCCTC TGGTCTCTCC 
GAGCACTCTG AGCCCAGTCT TAGCACTGAA GACAGCGTCT TGGATGCCCA ACGCAGCCTT 
CCAGCTGTGT TTCGGCCTGG GGACAGCAGC GTGGAAGATG GGCAGAGCAG CGAAGCCACC 
ACATCCCGGG ACGAGGCCCC TCGGGAGGAG CTGGCCGTGC AAGATAGCCT TGAGAGTGAC 
CTCCTTGCCA ACGAGAGCTT GGAGGAGTTC ATGTCTATCC CTGGCAGCCT GGACGTGGCC 
CTACCTGAGA AGGATGGCGC GGTGATGGAC GGCTGGCCCG GTGAGGCGGA CAAGCCCAGT 
CGGGCTGACA GTGAGGACAA CCTCTCAGAA GAGCCTGAGA TGGAGAGCCT GTTCCCTGCC 
CTGGCTTCTC TGGCTGTGAC CTCCTCTGCC AATAACGAGG CATCCCCTGT GTCCTCCAGT 
GGAGTCACCT ATTCTCCAGA ACTACTGGAC CTGTACACCG TGAACCTGCA CCGCATTGAG 
AAAGATGTGC AGAGGTGTGA CCGCAGCTAC TGGTACTTCA CAGCAGCCAA CCTGGAGAAG 
CTGAGGAACA TCATGTGCAG CTATATCTGG CAGCACATCG AGATCGGCTA CGTCCAGGGC 
ATGTGTGACC TCCTGGCCCC GTTGCTGGTC ATCCTGGATG ATGAGGCCCT GGCCTTCAGT 
TGCTTCACGG AGCTGATGAA GAGGATGAAC CAAAACTTCC CCCATGGAGG CGCCATGGAT 
ACGCACTTCG CCAACATGAG GTCTCTGATC CAGATCCTGG ACTCCGAGCT CTTCGAGCTG 
ATGCATCAGA ACGGCGATTA CACCCACTTC TACTTTTGCT ACCGCTGGTT CCTGCTGGAT 
TTCAAACGAG AGCTGGTCTA TGATGACGTC TTCTCCGTGT GGGAAACTAT CTGGGCTGCT 
AAGCACGTGT CCTCCGCCCA CTACGTGCTG TTCATCGCGC TGGCGCTGGT GGAGGTCTAC 
AGAGACATCA TCCTGGAGAA CAATATGGAC TTCACTGACA TTATCAAGTT CTTCAACGAA 
ATGGCTGAGC GGCACAACGC CAAGCAGATT CTGCAGCTCG CCCGGGACCT CGTGCATAAG 
GTGCAGATTC TAATTGAGAA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535185.1
CDS	97..3543
Translation	MASVPAEAETRQRLLRTVKKEVKQIMEEAVTRKFVHEDSSHIISFCAAVEACVLHGLRRRAAGFLRSNKIAALFMKVGKGFPPAEELSRKVQELEQLIESARNQIQGLQENVRKLPKLPNLSPLAIKHLWIRTALFERVLDKIVHYLVENSSKYYEKEALLMDPVDGPILASLLVGPCALEYTKMKTADHFWTDPSADELVQRHRIHSSHLRQDSPTKRPALCIQKRHSSGSMDDRPSISARDYVESLHQNSRATLLYGKNNVLVQPRDDMEAVPGYLSLHQTADVMTLKWTPNQLMNGSVGDLDYEKSVYWDYAVTIRLEEIVYLHCHQQVDSGGTVVLVSQDGIQRPPFRFPKGGHLLQFLSCLENGLLPHGQLDPPLWSQRGKGKVFPKLRKRSPQGSSESTSSDKEDDEATDYVFRIIYPGTQSEFVALDLMGSPRPVSIGPAWMMLAAGQSMLVVAKGLQWAQTRGCLTIPTRSVKEQPPMPQDLMDVSMNNLPPLWQPSPRKSSCSSCSQSGSADGGSTNGCNHERAPLKLLCDNMKYQILSRAFYGWLAYCRHLSTVRTHLSALVNHMIVSPDLPCDAGQGLTASIWEKYIQDSTTYPEQELLRLIYYGGVQPEIRRAVWPFLLGHYQFGMTEMERKEVDEQIHACYAQTMSEWLGCEAIVRQRERESHAAALAKCSSGASLDSHLHRMLHRDSTISNESSQSCSSGRQNLRLQSDSSSSTQVFESVDEVEQTEAEGRSEEKHPKIPNGNPANGTCSPDSGHPSSHNFSSGLSEHSEPSLSTEDSVLDAQRSLPAVFRPGDSSVEDGQSSEATTSRDEAPREELAVQDSLESDLLANESLEEFMSIPGSLDVALPEKDGAVMDGWPGEADKPSRADSEDNLSEEPEMESLFPALASLAVTSSANNEASPVSSSGVTYSPELLDLYTVNLHRIEKDVQRCDRSYWYFTAANLEKLRNIMCSYIWQHIEIGYVQGMCDLLAPLLVILDDEALAFSCFTELMKRMNQNFPHGGAMDTHFANMRSLIQILDSELFELMHQNGDYTHFYFCYRWFLLDFKRELVYDDVFSVWETIWAAKHVSSAHYVLFIALALVEVYRDIILENNMDFTDIIKFFNEMAERHNAKQILQLARDLVHKVQILIENK

Target ORF information:

RefSeq Version	XM_006535122.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535122.4

ORF Insert Sequence:

ATGGCTTCGG TCCCTGCGGA GGCCGAGACC CGCCAGAGGC TGCTGCGCAC TGTGAAGAAG 
GAGGTGAAGC AGATCATGGA AGAAGCCGTT ACCAGAAAGT TCGTCCACGA GGACAGCAGC 
CACATCATCT CCTTCTGTGC GGCCGTGGAG GCCTGCGTGC TGCACGGGCT GCGGCGGAGG 
GCGGCCGGCT TCCTGCGAAG CAACAAGATC GCGGCGCTCT TCATGAAGGT GGGCAAAGGC 
TTCCCTCCGG CCGAGGAGCT GAGCCGCAAG GTCCAGGAAC TGGAGCAACT CATTGAGAGC 
GCGCGAAACC AGATCCAGGG CCTGCAAGAG AACGTGCGGA AGCTGCCGAA GCTGCCTAAC 
CTGTCCCCGC TGGCCATCAA GCACCTGTGG ATCCGCACCG CCCTGTTCGA GAGGGTCCTG 
GACAAAATCG TCCACTACCT GGTGGAAAAC AGCAGTAAAT ACTACGAGAA GGAGGCACTC 
TTGATGGACC CGGTGGACGG ACCCATCCTG GCCTCTTTGT TGGTGGGGCC ATGTGCCCTG 
GAGTATACCA AGATGAAGAC TGCAGATCAC TTCTGGACTG ACCCCTCAGC CGACGAGCTG 
GTCCAGAGAC ACCGGATCCA CAGCTCACAT CTGAGACAGG ACTCGCCCAC CAAGCGGCCA 
GCGCTCTGTA TCCAGAAGAG GCATTCCAGT GGCAGCATGG ACGACCGGCC ATCTATCTCT 
GCCCGGGACT ATGTAGAATC TCTGCACCAG AACTCCAGGG CCACCCTGCT CTATGGAAAG 
AACAATGTTC TGGTTCAGCC AAGGGATGAC ATGGAGGCCG TGCCAGGGTA CCTGTCTCTG 
CACCAGACAG CGGATGTCAT GACCTTGAAG TGGACACCCA ATCAGCTGAT GAACGGGTCT 
GTGGGGGATC TGGACTACGA GAAGAGTGTC TACTGGGACT ACGCTGTGAC CATCCGCTTA 
GAGGAGATAG TTTACCTGCA CTGCCATCAA CAAGTGGACA GCGGCGGGAC TGTCGTGCTG 
GTGAGCCAGG ATGGAATCCA GAGGCCACCC TTCCGCTTCC CCAAGGGCGG GCACCTGCTA 
CAGTTCCTCT CCTGTCTGGA GAATGGGCTG CTTCCGCACG GGCAGCTGGA CCCGCCGCTT 
TGGTCACAGC GAGGAAAGGG GAAGGTATTT CCTAAGTTGC GCAAGCGCAG CCCACAGGGG 
TCCTCCGAGT CCACGTCTTC AGACAAGGAG GACGACGAAG CCACGGATTA CGTGTTCCGC 
ATCATCTACC CTGGCACGCA GTCTGAATTC GTTGCCCTTG ACCTCATGGG CAGCCCCCGG 
CCTGTGTCCA TTGGCCCTGC CTGGATGATG CTTGCTGCGG GCCAGTCCAT GCTAGTGGTG 
GCCAAGGGGC TCCAGTGGGC ACAAACCAGA GGGTGCCTCA CCATCCCCAC AAGGAGTGTG 
AAGGAGCAGC CCCCCATGCC CCAGGACCTA ATGGATGTCT CTATGAACAA CCTTCCACCC 
CTATGGCAAC CCAGTCCTCG GAAGTCCTCC TGCTCTTCCT GTTCACAAAG CGGCTCAGCT 
GACGGCGGCT CAACCAATGG CTGTAACCAT GAGAGGGCCC CACTGAAACT GCTGTGTGAT 
AACATGAAGT ACCAGATCCT CTCCAGAGCC TTCTATGGAT GGCTCGCCTA CTGCAGACAC 
CTGTCCACCG TGAGGACCCA CCTGTCAGCC CTGGTCAATC ACATGATCGT GTCTCCAGAC 
CTGCCCTGTG ACGCTGGGCA AGGGTTGACA GCCAGCATCT GGGAAAAATA CATCCAGGAC 
AGCACGACCT ACCCGGAGCA GGAGCTGCTG CGTCTCATCT ACTATGGAGG TGTCCAGCCT 
GAGATCCGCA GGGCAGTGTG GCCCTTCCTC CTCGGCCACT ACCAGTTTGG GATGACAGAG 
ATGGAGAGGA AAGAGGTAGA CGAACAGATC CATGCCTGCT ACGCACAAAC CATGTCGGAG 
TGGCTGGGCT GTGAAGCTAT CGTGAGACAG AGGGAGCGGG AGTCCCACGC GGCTGCCCTG 
GCCAAGTGCT CTTCAGGAGC CAGCCTGGAC AGCCACCTTC ACCGGATGCT GCACCGGGAC 
TCCACCATCA GCAATGAGTC ATCCCAGAGC TGCAGCTCAG GCCGCCAGAA CCTCCGACTG 
CAGAGCGACT CCAGCAGCAG CACACAGGTA TTTGAGTCTG TGGATGAGGT GGAGCAGACA 
GAGGCAGAAG GCAGGTCAGA AGAAAAACAT CCCAAAATCC CCAATGGGAA CCCAGCCAAC 
GGCACTTGCT CCCCAGACTC CGGACACCCT TCCTCCCACA ACTTCTCCTC TGGTCTCTCC 
GAGCACTCTG AGCCCAGTCT TAGCACTGAA GACAGCGTCT TGGATGCCCA ACGCAGCCTT 
CCAGCTGTGT TTCGGCCTGG GGACAGCAGC GTGGAAGATG GGCAGAGCAG CGAAGCCACC 
ACATCCCGGG ACGAGGCCCC TCGGGAGGAG CTGGCCGTGC AAGATAGCCT TGAGAGTGAC 
CTCCTTGCCA ACGAGAGCTT GGAGGAGTTC ATGTCTATCC CTGGCAGCCT GGACGTGGCC 
CTACCTGAGA AGGATGGCGC GGTGATGGAC GGCTGGCCCG GTGAGGCGGA CAAGCCCAGT 
CGGGCTGACA GTGAGGACAA CCTCTCAGAA GAGCCTGAGA TGGAGAGCCT GTTCCCTGCC 
CTGGCTTCTC TGGCTGTGAC CTCCTCTGCC AATAACGAGG CATCCCCTGT GTCCTCCAGT 
GGAGTCACCT ATTCTCCAGA ACTACTGGAC CTGTACACCG TGAACCTGCA CCGCATTGAG 
AAAGATGTGC AGAGGTGTGA CCGCAGCTAC TGGTACTTCA CAGCAGCCAA CCTGGAGAAG 
CTGAGGAACA TCATGTGCAG CTATATCTGG CAGCACATCG AGATCGGCTA CGTCCAGGGC 
ATGTGTGACC TCCTGGCCCC GTTGCTGGTC ATCCTGGATG ATGAGGCCCT GGCCTTCAGT 
TGCTTCACGG AGCTGATGAA GAGGATGAAC CAAAACTTCC CCCATGGAGG CGCCATGGAT 
ACGCACTTCG CCAACATGAG GTCTCTGATC CAGATCCTGG ACTCCGAGCT CTTCGAGCTG 
ATGCATCAGA ACGGCGATTA CACCCACTTC TACTTTTGCT ACCGCTGGTT CCTGCTGGAT 
TTCAAACGAG AGCTGGTCTA TGATGACGTC TTCTCCGTGT GGGAAACTAT CTGGGCTGCT 
AAGCACGTGT CCTCCGCCCA CTACGTGCTG TTCATCGCGC TGGCGCTGGT GGAGGTCTAC 
AGAGACATCA TCCTGGAGAA CAATATGGAC TTCACTGACA TTATCAAGTT CTTCAACGAA 
ATGGCTGAGC GGCACAACGC CAAGCAGATT CTGCAGCTCG CCCGGGACCT CGTGCATAAG 
GTGCAGATTC TAATTGAGAA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sgsm1 ( NM_172718.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766306.2 Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 1, mRNA.

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CloneID	OMu03947
Clone ID Related Accession (Same CDS sequence)	NM_172718.3
Accession Version	NM_172718.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	small G protein signaling modulator 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC130548.3, AK053555.1, AK044716.1, AK167632.1 and BP761545.1. On Jul 2, 2009 this sequence version replaced NM_172718.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK053555.1, SRR1660815.164090.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCGG TCCCTGCGGA GGCCGAGACC CGCCAGAGGC TGCTGCGCAC TGTGAAGAAG 
GAGGTGAAGC AGATCATGGA AGAAGCCGTT ACCAGAAAGT TCGTCCACGA GGACAGCAGC 
CACATCATCT CCTTCTGTGC GGCCGTGGAG GCCTGCGTGC TGCACGGGCT GCGGCGGAGG 
GCGGCCGGCT TCCTGCGAAG CAACAAGATC GCGGCGCTCT TCATGAAGGT GGGCAAAGGC 
TTCCCTCCGG CCGAGGAGCT GAGCCGCAAG GTCCAGGAAC TGGAGCAACT CATTGAGAGC 
GCGCGAAACC AGATCCAGGG CCTGCAAGAG AACGTGCGGA AGCTGCCGAA GCTGCCTAAC 
CTGTCCCCGC TGGCCATCAA GCACCTGTGG ATCCGCACCG CCCTGTTCGA GAGGGTCCTG 
GACAAAATCG TCCACTACCT GGTGGAAAAC AGCAGTAAAT ACTACGAGAA GGAGGCACTC 
TTGATGGACC CGGTGGACGG ACCCATCCTG GCCTCTTTGT TGGTGGGGCC ATGTGCCCTG 
GAGTATACCA AGATGAAGAC TGCAGATCAC TTCTGGACTG ACCCCTCAGC CGACGAGCTG 
GTCCAGAGAC ACCGGATCCA CAGCTCACAT CTGAGACAGG ACTCGCCCAC CAAGCGGCCA 
GCGCTCTGTA TCCAGAAGAG GCATTCCAGT GGCAGCATGG ACGACCGGCC ATCTATCTCT 
GCCCGGGACT ATGTAGAATC TCTGCACCAG AACTCCAGGG CCACCCTGCT CTATGGAAAG 
AACAATGTTC TGGTTCAGCC AAGGGATGAC ATGGAGGCCG TGCCAGGGTA CCTGTCTCTG 
CACCAGACAG CGGATGTCAT GACCTTGAAG TGGACACCCA ATCAGCTGAT GAACGGGTCT 
GTGGGGGATC TGGACTACGA GAAGAGTGTC TACTGGGACT ACGCTGTGAC CATCCGCTTA 
GAGGAGATAG TTTACCTGCA CTGCCATCAA CAAGTGGACA GCGGCGGGAC TGTCGTGCTG 
GTGAGCCAGG ATGGAATCCA GAGGCCACCC TTCCGCTTCC CCAAGGGCGG GCACCTGCTA 
CAGTTCCTCT CCTGTCTGGA GAATGGGCTG CTTCCGCACG GGCAGCTGGA CCCGCCGCTT 
TGGTCACAGC GAGGAAAGGG GAAGGTATTT CCTAAGTTGC GCAAGCGCAG CCCACAGGGG 
TCCTCCGAGT CCACGTCTTC AGACAAGGAG GACGACGAAG CCACGGATTA CGTGTTCCGC 
ATCATCTACC CTGGCACGCA GTCTGAATTC GTGCCCCAGG ACCTAATGGA TGTCTCTATG 
AACAACCTTC CACCCCTATG GCAACCCAGT CCTCGGAAGT CCTCCTGCTC TTCCTGTTCA 
CAAAGCGGCT CAGCTGACGG CGGCTCAACC AATGGCTGTA ACCATGAGAG GGCCCCACTG 
AAACTGCTGT GTGATAACAT GAAGTACCAG ATCCTCTCCA GAGCCTTCTA TGGATGGCTC 
GCCTACTGCA GACACCTGTC CACCGTGAGG ACCCACCTGT CAGCCCTGGT CAATCACATG 
ATCGTGTCTC CAGACCTGCC CTGTGACGCT GGGCAAGGGT TGACAGCCAG CATCTGGGAA 
AAATACATCC AGGACAGCAC GACCTACCCG GAGCAGGAGC TGCTGCGTCT CATCTACTAT 
GGAGGTGTCC AGCCTGAGAT CCGCAGGGCA GTGTGGCCCT TCCTCCTCGG CCACTACCAG 
TTTGGGATGA CAGAGATGGA GAGGAAAGAG GTAGACGAAC AGATCCATGC CTGCTACGCA 
CAAACCATGT CGGAGTGGCT GGGCTGTGAA GCTATCGTGA GACAGAGGGA GCGGGAGTCC 
CACGCGGCTG CCCTGGCCAA GTGCTCTTCA GGAGCCAGCC TGGACAGCCA CCTTCACCGG 
ATGCTGCACC GGGACTCCAC CATCAGCAAT GAGTCATCCC AGAGCTGCAG CTCAGGCCGC 
CAGAACCTCC GACTGCAGAG CGACTCCAGC AGCAGCACAC AGGTATTTGA GTCTGTGGAT 
GAGGTGGAGC AGACAGAGGC AGAAGGCAGG TCAGAAGAAA AACATCCCAA AATCCCCAAT 
GGGAACCCAG CCAACGGCAC TTGCTCCCCA GACTCCGGAC ACCCTTCCTC CCACAACTTC 
TCCTCTGGTC TCTCCGAGCA CTCTGAGCCC AGTCTTAGCA CTGAAGACAG CGTCTTGGAT 
GCCCAACGCA GCCTTCCAGC TGTGTTTCGG CCTGGGGACA GCAGCGTGGA AGATGGGCAG 
AGCAGCGAAG CCACCACATC CCGGGACGAG GCCCCTCGGG AGGAGCTGGC CGTGCAAGAT 
AGCCTTGAGA GTGACCTCCT TGCCAACGAG AGCTTGGAGG AGTTCATGTC TATCCCTGGC 
AGCCTGGACG TGGCCCTACC TGAGAAGGAT GGCGCGGTGA TGGACGGCTG GCCCGGTGAG 
GCGGACAAGC CCAGTCGGGC TGACAGTGAG GACAACCTCT CAGAAGAGCC TGAGATGGAG 
AGCCTGTTCC CTGCCCTGGC TTCTCTGGCT GTGACCTCCT CTGCCAATAA CGAGGCATCC 
CCTGTGTCCT CCAGTGGAGT CACCTATTCT CCAGAACTAC TGGACCTGTA CACCGTGAAC 
CTGCACCGCA TTGAGAAAGA TGTGCAGAGG TGTGACCGCA GCTACTGGTA CTTCACAGCA 
GCCAACCTGG AGAAGCTGAG GAACATCATG TGCAGCTATA TCTGGCAGCA CATCGAGATC 
GGCTACGTCC AGGGCATGTG TGACCTCCTG GCCCCGTTGC TGGTCATCCT GGATGATGAG 
GCCCTGGCCT TCAGTTGCTT CACGGAGCTG ATGAAGAGGA TGAACCAAAA CTTCCCCCAT 
GGAGGCGCCA TGGATACGCA CTTCGCCAAC ATGAGGTCTC TGATCCAGAT CCTGGACTCC 
GAGCTCTTCG AGCTGATGCA TCAGAACGGC GATTACACCC ACTTCTACTT TTGCTACCGC 
TGGTTCCTGC TGGATTTCAA ACGAGAGCTG GTCTATGATG ACGTCTTCTC CGTGTGGGAA 
ACTATCTGGG CTGCTAAGCA CGTGTCCTCC GCCCACTACG TGCTGTTCAT CGCGCTGGCG 
CTGGTGGAGG TCTACAGAGA CATCATCCTG GAGAACAATA TGGACTTCAC TGACATTATC 
AAGTTCTTCA ACGAAATGGC TGAGCGGCAC AACGCCAAGC AGATTCTGCA GCTCGCCCGG 
GACCTCGTGC ATAAGGTGCA GATTCTAATT GAGAACAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766306.2
CDS	95..3376
Translation	MASVPAEAETRQRLLRTVKKEVKQIMEEAVTRKFVHEDSSHIISFCAAVEACVLHGLRRRAAGFLRSNKIAALFMKVGKGFPPAEELSRKVQELEQLIESARNQIQGLQENVRKLPKLPNLSPLAIKHLWIRTALFERVLDKIVHYLVENSSKYYEKEALLMDPVDGPILASLLVGPCALEYTKMKTADHFWTDPSADELVQRHRIHSSHLRQDSPTKRPALCIQKRHSSGSMDDRPSISARDYVESLHQNSRATLLYGKNNVLVQPRDDMEAVPGYLSLHQTADVMTLKWTPNQLMNGSVGDLDYEKSVYWDYAVTIRLEEIVYLHCHQQVDSGGTVVLVSQDGIQRPPFRFPKGGHLLQFLSCLENGLLPHGQLDPPLWSQRGKGKVFPKLRKRSPQGSSESTSSDKEDDEATDYVFRIIYPGTQSEFVPQDLMDVSMNNLPPLWQPSPRKSSCSSCSQSGSADGGSTNGCNHERAPLKLLCDNMKYQILSRAFYGWLAYCRHLSTVRTHLSALVNHMIVSPDLPCDAGQGLTASIWEKYIQDSTTYPEQELLRLIYYGGVQPEIRRAVWPFLLGHYQFGMTEMERKEVDEQIHACYAQTMSEWLGCEAIVRQRERESHAAALAKCSSGASLDSHLHRMLHRDSTISNESSQSCSSGRQNLRLQSDSSSSTQVFESVDEVEQTEAEGRSEEKHPKIPNGNPANGTCSPDSGHPSSHNFSSGLSEHSEPSLSTEDSVLDAQRSLPAVFRPGDSSVEDGQSSEATTSRDEAPREELAVQDSLESDLLANESLEEFMSIPGSLDVALPEKDGAVMDGWPGEADKPSRADSEDNLSEEPEMESLFPALASLAVTSSANNEASPVSSSGVTYSPELLDLYTVNLHRIEKDVQRCDRSYWYFTAANLEKLRNIMCSYIWQHIEIGYVQGMCDLLAPLLVILDDEALAFSCFTELMKRMNQNFPHGGAMDTHFANMRSLIQILDSELFELMHQNGDYTHFYFCYRWFLLDFKRELVYDDVFSVWETIWAAKHVSSAHYVLFIALALVEVYRDIILENNMDFTDIIKFFNEMAERHNAKQILQLARDLVHKVQILIENK

Target ORF information:

RefSeq Version	NM_172718.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172718.3

ORF Insert Sequence:

ATGGCTTCGG TCCCTGCGGA GGCCGAGACC CGCCAGAGGC TGCTGCGCAC TGTGAAGAAG 
GAGGTGAAGC AGATCATGGA AGAAGCCGTT ACCAGAAAGT TCGTCCACGA GGACAGCAGC 
CACATCATCT CCTTCTGTGC GGCCGTGGAG GCCTGCGTGC TGCACGGGCT GCGGCGGAGG 
GCGGCCGGCT TCCTGCGAAG CAACAAGATC GCGGCGCTCT TCATGAAGGT GGGCAAAGGC 
TTCCCTCCGG CCGAGGAGCT GAGCCGCAAG GTCCAGGAAC TGGAGCAACT CATTGAGAGC 
GCGCGAAACC AGATCCAGGG CCTGCAAGAG AACGTGCGGA AGCTGCCGAA GCTGCCTAAC 
CTGTCCCCGC TGGCCATCAA GCACCTGTGG ATCCGCACCG CCCTGTTCGA GAGGGTCCTG 
GACAAAATCG TCCACTACCT GGTGGAAAAC AGCAGTAAAT ACTACGAGAA GGAGGCACTC 
TTGATGGACC CGGTGGACGG ACCCATCCTG GCCTCTTTGT TGGTGGGGCC ATGTGCCCTG 
GAGTATACCA AGATGAAGAC TGCAGATCAC TTCTGGACTG ACCCCTCAGC CGACGAGCTG 
GTCCAGAGAC ACCGGATCCA CAGCTCACAT CTGAGACAGG ACTCGCCCAC CAAGCGGCCA 
GCGCTCTGTA TCCAGAAGAG GCATTCCAGT GGCAGCATGG ACGACCGGCC ATCTATCTCT 
GCCCGGGACT ATGTAGAATC TCTGCACCAG AACTCCAGGG CCACCCTGCT CTATGGAAAG 
AACAATGTTC TGGTTCAGCC AAGGGATGAC ATGGAGGCCG TGCCAGGGTA CCTGTCTCTG 
CACCAGACAG CGGATGTCAT GACCTTGAAG TGGACACCCA ATCAGCTGAT GAACGGGTCT 
GTGGGGGATC TGGACTACGA GAAGAGTGTC TACTGGGACT ACGCTGTGAC CATCCGCTTA 
GAGGAGATAG TTTACCTGCA CTGCCATCAA CAAGTGGACA GCGGCGGGAC TGTCGTGCTG 
GTGAGCCAGG ATGGAATCCA GAGGCCACCC TTCCGCTTCC CCAAGGGCGG GCACCTGCTA 
CAGTTCCTCT CCTGTCTGGA GAATGGGCTG CTTCCGCACG GGCAGCTGGA CCCGCCGCTT 
TGGTCACAGC GAGGAAAGGG GAAGGTATTT CCTAAGTTGC GCAAGCGCAG CCCACAGGGG 
TCCTCCGAGT CCACGTCTTC AGACAAGGAG GACGACGAAG CCACGGATTA CGTGTTCCGC 
ATCATCTACC CTGGCACGCA GTCTGAATTC GTGCCCCAGG ACCTAATGGA TGTCTCTATG 
AACAACCTTC CACCCCTATG GCAACCCAGT CCTCGGAAGT CCTCCTGCTC TTCCTGTTCA 
CAAAGCGGCT CAGCTGACGG CGGCTCAACC AATGGCTGTA ACCATGAGAG GGCCCCACTG 
AAACTGCTGT GTGATAACAT GAAGTACCAG ATCCTCTCCA GAGCCTTCTA TGGATGGCTC 
GCCTACTGCA GACACCTGTC CACCGTGAGG ACCCACCTGT CAGCCCTGGT CAATCACATG 
ATCGTGTCTC CAGACCTGCC CTGTGACGCT GGGCAAGGGT TGACAGCCAG CATCTGGGAA 
AAATACATCC AGGACAGCAC GACCTACCCG GAGCAGGAGC TGCTGCGTCT CATCTACTAT 
GGAGGTGTCC AGCCTGAGAT CCGCAGGGCA GTGTGGCCCT TCCTCCTCGG CCACTACCAG 
TTTGGGATGA CAGAGATGGA GAGGAAAGAG GTAGACGAAC AGATCCATGC CTGCTACGCA 
CAAACCATGT CGGAGTGGCT GGGCTGTGAA GCTATCGTGA GACAGAGGGA GCGGGAGTCC 
CACGCGGCTG CCCTGGCCAA GTGCTCTTCA GGAGCCAGCC TGGACAGCCA CCTTCACCGG 
ATGCTGCACC GGGACTCCAC CATCAGCAAT GAGTCATCCC AGAGCTGCAG CTCAGGCCGC 
CAGAACCTCC GACTGCAGAG CGACTCCAGC AGCAGCACAC AGGTATTTGA GTCTGTGGAT 
GAGGTGGAGC AGACAGAGGC AGAAGGCAGG TCAGAAGAAA AACATCCCAA AATCCCCAAT 
GGGAACCCAG CCAACGGCAC TTGCTCCCCA GACTCCGGAC ACCCTTCCTC CCACAACTTC 
TCCTCTGGTC TCTCCGAGCA CTCTGAGCCC AGTCTTAGCA CTGAAGACAG CGTCTTGGAT 
GCCCAACGCA GCCTTCCAGC TGTGTTTCGG CCTGGGGACA GCAGCGTGGA AGATGGGCAG 
AGCAGCGAAG CCACCACATC CCGGGACGAG GCCCCTCGGG AGGAGCTGGC CGTGCAAGAT 
AGCCTTGAGA GTGACCTCCT TGCCAACGAG AGCTTGGAGG AGTTCATGTC TATCCCTGGC 
AGCCTGGACG TGGCCCTACC TGAGAAGGAT GGCGCGGTGA TGGACGGCTG GCCCGGTGAG 
GCGGACAAGC CCAGTCGGGC TGACAGTGAG GACAACCTCT CAGAAGAGCC TGAGATGGAG 
AGCCTGTTCC CTGCCCTGGC TTCTCTGGCT GTGACCTCCT CTGCCAATAA CGAGGCATCC 
CCTGTGTCCT CCAGTGGAGT CACCTATTCT CCAGAACTAC TGGACCTGTA CACCGTGAAC 
CTGCACCGCA TTGAGAAAGA TGTGCAGAGG TGTGACCGCA GCTACTGGTA CTTCACAGCA 
GCCAACCTGG AGAAGCTGAG GAACATCATG TGCAGCTATA TCTGGCAGCA CATCGAGATC 
GGCTACGTCC AGGGCATGTG TGACCTCCTG GCCCCGTTGC TGGTCATCCT GGATGATGAG 
GCCCTGGCCT TCAGTTGCTT CACGGAGCTG ATGAAGAGGA TGAACCAAAA CTTCCCCCAT 
GGAGGCGCCA TGGATACGCA CTTCGCCAAC ATGAGGTCTC TGATCCAGAT CCTGGACTCC 
GAGCTCTTCG AGCTGATGCA TCAGAACGGC GATTACACCC ACTTCTACTT TTGCTACCGC 
TGGTTCCTGC TGGATTTCAA ACGAGAGCTG GTCTATGATG ACGTCTTCTC CGTGTGGGAA 
ACTATCTGGG CTGCTAAGCA CGTGTCCTCC GCCCACTACG TGCTGTTCAT CGCGCTGGCG 
CTGGTGGAGG TCTACAGAGA CATCATCCTG GAGAACAATA TGGACTTCAC TGACATTATC 
AAGTTCTTCA ACGAAATGGC TGAGCGGCAC AACGCCAAGC AGATTCTGCA GCTCGCCCGG 
GACCTCGTGC ATAAGGTGCA GATTCTAATT GAGAACAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sgsm1 ( NM_001162965.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156437.1 Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu08639
Clone ID Related Accession (Same CDS sequence)	NM_001162965.1
Accession Version	NM_001162965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	small G protein signaling modulator 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC130548.3, AK053555.1, AK044716.1, AK051018.1 and AC151577.2. Transcript Variant: This variant (3) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (b) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK170021.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCTTCGG TCCCTGCGGA GGCCGAGACC CGCCAGAGGC TGCTGCGCAC TGTGAAGAAG 
GAGGTGAAGC AGATCATGGA AGAAGCCGTT ACCAGAAAGT TCGTCCACGA GGACAGCAGC 
CACATCATCT CCTTCTGTGC GGCCGTGGAG GCCTGCGTGC TGCACGGGCT GCGGCGGAGG 
GCGGCCGGCT TCCTGCGAAG CAACAAGATC GCGGCGCTCT TCATGAAGGT GGGCAAAGGC 
TTCCCTCCGG CCGAGGAGCT GAGCCGCAAG GTCCAGGAAC TGGAGCAACT CATTGAGAGC 
GCGCGAAACC AGATCCAGGG CCTGCAAGAG AACGTGCGGA AGCTGCCGAA GCTGCCTAAC 
CTGTCCCCGC TGGCCATCAA GCACCTGTGG ATCCGCACCG CCCTGTTCGA GAGGGTCCTG 
GACAAAATCG TCCACTACCT GGTGGAAAAC AGCAGTAAAT ACTACGAGAA GGAGGCACTC 
TTGATGGACC CGGTGGACGG ACCCATCCTG GCCTCTTTGT TGGTGGGGCC ATGTGCCCTG 
GAGTATACCA AGATGAAGAC TGCAGATCAC TTCTGGACTG ACCCCTCAGC CGACGAGCTG 
GTCCAGAGAC ACCGGATCCA CAGCTCACAT CTGAGACAGG ACTCGCCCAC CAAGCGGCCA 
GCGCTCTGTA TCCAGAAGAG GCATTCCAGT GGCAGCATGG ACGACCGGCC ATCTATCTCT 
GCCCGGGACT ATGTAGAATC TCTGCACCAG AACTCCAGGG CCACCCTGCT CTATGGAAAG 
AACAATGTTC TGGTTCAGCC AAGGGATGAC ATGGAGGCCG TGCCAGGGTA CCTGTCTCTG 
CACCAGACAG CGGATGTCAT GACCTTGAAG TGGACACCCA ATCAGCTGAT GAACGGGTCT 
GTGGGGGATC TGGACTACGA GAAGAGTGTC TACTGGGACT ACGCTGTGAC CATCCGCTTA 
GAGGAGATAG TTTACCTGCA CTGCCATCAA CAAGTGGACA GCGGCGGGAC TGTCGTGCTG 
GTGAGCCAGG ATGGAATCCA GAGGCCACCC TTCCGCTTCC CCAAGGGCGG GCACCTGCTA 
CAGTTCCTCT CCTGTCTGGA GAATGGGCTG CTTCCGCACG GGCAGCTGGA CCCGCCGCTT 
TGGTCACAGC GAGGAAAGGG GAAGGTATTT CCTAAGTTGC GCAAGCGCAG CCCACAGGGG 
TCCTCCGAGT CCACGTCTTC AGACAAGGAG GACGACGAAG CCACGGATTA CGTGTTCCGC 
ATCATCTACC CTGGCACGCA GTCTGAATTC GTGCCCCAGG ACCTAATGGA TGTCTCTATG 
AACAACCTTC CACCCCTATG GCAACCCAGT CCTCGGAAGT CCTCCTGCTC TTCCTGTTCA 
CAAAGCGGCT CAGCTGACGG CGGCTCAACC AATGGCTGTA ACCATGAGAG GGCCCCACTG 
AAACTGCTGT GTGATAACAT GAAGTACCAG ATCCTCTCCA GAGCCTTCTA TGGATGGCTC 
GCCTACTGCA GACACCTGTC CACCGTGAGG ACCCACCTGT CAGCCCTGGT CAATCACATG 
ATCGTGTCTC CAGACCTGCC CTGTGACGCT GGGCAAGGGT TGACAGCCAG CATCTGGGAA 
AAATACATCC AGGACAGCAC GACCTACCCG GAGCAGGAGC TGCTGCGTCT CATCTACTAT 
GGAGGTGTCC AGCCTGAGAT CCGCAGGGCA GTGTGGCCCT TCCTCCTCGG CCACTACCAG 
TTTGGGATGA CAGAGATGGA GAGGAAAGAG GTAGACGAAC AGATCCATGC CTGCTACGCA 
CAAACCATGT CGGAGTGGCT GGGCTGTGAA GCTATCGTGA GACAGAGGGA GCGGGAGTCC 
CACGCGGCTG CCCTGGCCAA GTGCTCTTCA GGAGCCAGCC TGGACAGCCA CCTTCACCGG 
ATGCTGCACC GGGACTCCAC CATCAGCAAT GAGTCATCCC AGAGCTGCAG CTCAGGCCGC 
CAGAACCTCC GACTGCAGAG CGACTCCAGC AGCAGCACAC AGGTATTTGA GTCTGTGGAT 
GAGGTGGAGC AGACAGAGGC AGAAGGCAGG TCAGAAGAAA AACATCCCAA AATCCCCAAT 
GGGAACCCAG CCAACGGCAC TTGCTCCCCA GACTCCGGAC ACCCTTCCTC CCACAACTTC 
TCCTCTGGTC TCTCCGAGCA CTCTGAGCCC AGTCTTAGCA CTGAAGACAG CGTCTTGGAT 
GCCCAACGCA GCCTTCCAGC TGTGTTTCGG CCTGGGGACA GCAGCGTGGA AGATGGGCAG 
AGCAGCGAAG CCACCACATC CCGGGACGAG GCCCCTCGGG AGGAGCTGGC CGTGCAAGAT 
AGCCTTGAGA GTGACCTCCT TGCCAACGAG AGCTTGGAGG AGTTCATGTC TATCCCTGGC 
AGCCTGGACG TGGCCCTACC TGAGAAGGAT GGCGCGGTGA TGGACGGCTG GCCCGGTGAG 
GCGGACAAGC CCAGTCGGGC TGACAGTGAG GACAACCTCT CAGAAGAGCC TGAGATGGAG 
AGCCTGTTCC CTGCCCTGGC TTCTCTGGCT GTGACCTCCT CTGCCAATAA CGAGGCATCC 
CCTGTGTCCT CCAGTGGAGT CACCTATTCT CCAGAACTAC TGGACCTGTA CACCGTGAAC 
CTGCACCGCA TTGAGAAAGA TGTGCAGAGG TGTGACCGCA GCTACTGGTA CTTCACAGCA 
GCCAACCTGG AGAAGCTGAG GAACATCATG TGCAGCTATA TCTGGCAGCA CATCGAGATC 
GGCTACGTCC AGGGCATGTG TGACCTCCTG GCCCCGTTGC TGGTCATCCT GGATGATGAC 
CAGGTTTCTC TGTATAGCCC CAGCTGTCCT GGAACTCAAG TTGTAGACCA GGCTGGCCTC 
GAACTCAGAA ATCCGCCTGC CTTTGCCTTC CCAATGCTGG GATTAGAGGC ATGCGCCACC 
ACCGCCCGGC TAGATAATTG GAATGTTAGC AGCTCACTTG GCGATACCAG TCCTCACCGG 
CATCTCACAT TTACCCTGAT GTCTACGTCA GACAGAATCA TTCCAGGGAA ATGTCAGACT 
AGTACCTCTC ATGAATATAA AAGCAAAACG TCTCAAAAAA AAATCAACAA ACTCAATCCA 
AGAAGATATA AAATATTACA CTGCATGGCC AAGCGGGTCT TATCCCAGAA ACGCAAGGGT 
GGTTCAATAT CCAAGCATCA ATCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156437.1
CDS	95..3304
Translation	MASVPAEAETRQRLLRTVKKEVKQIMEEAVTRKFVHEDSSHIISFCAAVEACVLHGLRRRAAGFLRSNKIAALFMKVGKGFPPAEELSRKVQELEQLIESARNQIQGLQENVRKLPKLPNLSPLAIKHLWIRTALFERVLDKIVHYLVENSSKYYEKEALLMDPVDGPILASLLVGPCALEYTKMKTADHFWTDPSADELVQRHRIHSSHLRQDSPTKRPALCIQKRHSSGSMDDRPSISARDYVESLHQNSRATLLYGKNNVLVQPRDDMEAVPGYLSLHQTADVMTLKWTPNQLMNGSVGDLDYEKSVYWDYAVTIRLEEIVYLHCHQQVDSGGTVVLVSQDGIQRPPFRFPKGGHLLQFLSCLENGLLPHGQLDPPLWSQRGKGKVFPKLRKRSPQGSSESTSSDKEDDEATDYVFRIIYPGTQSEFVPQDLMDVSMNNLPPLWQPSPRKSSCSSCSQSGSADGGSTNGCNHERAPLKLLCDNMKYQILSRAFYGWLAYCRHLSTVRTHLSALVNHMIVSPDLPCDAGQGLTASIWEKYIQDSTTYPEQELLRLIYYGGVQPEIRRAVWPFLLGHYQFGMTEMERKEVDEQIHACYAQTMSEWLGCEAIVRQRERESHAAALAKCSSGASLDSHLHRMLHRDSTISNESSQSCSSGRQNLRLQSDSSSSTQVFESVDEVEQTEAEGRSEEKHPKIPNGNPANGTCSPDSGHPSSHNFSSGLSEHSEPSLSTEDSVLDAQRSLPAVFRPGDSSVEDGQSSEATTSRDEAPREELAVQDSLESDLLANESLEEFMSIPGSLDVALPEKDGAVMDGWPGEADKPSRADSEDNLSEEPEMESLFPALASLAVTSSANNEASPVSSSGVTYSPELLDLYTVNLHRIEKDVQRCDRSYWYFTAANLEKLRNIMCSYIWQHIEIGYVQGMCDLLAPLLVILDDDQVSLYSPSCPGTQVVDQAGLELRNPPAFAFPMLGLEACATTARLDNWNVSSSLGDTSPHRHLTFTLMSTSDRIIPGKCQTSTSHEYKSKTSQKKINKLNPRRYKILHCMAKRVLSQKRKGGSISKHQSV

Target ORF information:

RefSeq Version	NM_001162965.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001162965.1

ORF Insert Sequence:

ATGGCTTCGG TCCCTGCGGA GGCCGAGACC CGCCAGAGGC TGCTGCGCAC TGTGAAGAAG 
GAGGTGAAGC AGATCATGGA AGAAGCCGTT ACCAGAAAGT TCGTCCACGA GGACAGCAGC 
CACATCATCT CCTTCTGTGC GGCCGTGGAG GCCTGCGTGC TGCACGGGCT GCGGCGGAGG 
GCGGCCGGCT TCCTGCGAAG CAACAAGATC GCGGCGCTCT TCATGAAGGT GGGCAAAGGC 
TTCCCTCCGG CCGAGGAGCT GAGCCGCAAG GTCCAGGAAC TGGAGCAACT CATTGAGAGC 
GCGCGAAACC AGATCCAGGG CCTGCAAGAG AACGTGCGGA AGCTGCCGAA GCTGCCTAAC 
CTGTCCCCGC TGGCCATCAA GCACCTGTGG ATCCGCACCG CCCTGTTCGA GAGGGTCCTG 
GACAAAATCG TCCACTACCT GGTGGAAAAC AGCAGTAAAT ACTACGAGAA GGAGGCACTC 
TTGATGGACC CGGTGGACGG ACCCATCCTG GCCTCTTTGT TGGTGGGGCC ATGTGCCCTG 
GAGTATACCA AGATGAAGAC TGCAGATCAC TTCTGGACTG ACCCCTCAGC CGACGAGCTG 
GTCCAGAGAC ACCGGATCCA CAGCTCACAT CTGAGACAGG ACTCGCCCAC CAAGCGGCCA 
GCGCTCTGTA TCCAGAAGAG GCATTCCAGT GGCAGCATGG ACGACCGGCC ATCTATCTCT 
GCCCGGGACT ATGTAGAATC TCTGCACCAG AACTCCAGGG CCACCCTGCT CTATGGAAAG 
AACAATGTTC TGGTTCAGCC AAGGGATGAC ATGGAGGCCG TGCCAGGGTA CCTGTCTCTG 
CACCAGACAG CGGATGTCAT GACCTTGAAG TGGACACCCA ATCAGCTGAT GAACGGGTCT 
GTGGGGGATC TGGACTACGA GAAGAGTGTC TACTGGGACT ACGCTGTGAC CATCCGCTTA 
GAGGAGATAG TTTACCTGCA CTGCCATCAA CAAGTGGACA GCGGCGGGAC TGTCGTGCTG 
GTGAGCCAGG ATGGAATCCA GAGGCCACCC TTCCGCTTCC CCAAGGGCGG GCACCTGCTA 
CAGTTCCTCT CCTGTCTGGA GAATGGGCTG CTTCCGCACG GGCAGCTGGA CCCGCCGCTT 
TGGTCACAGC GAGGAAAGGG GAAGGTATTT CCTAAGTTGC GCAAGCGCAG CCCACAGGGG 
TCCTCCGAGT CCACGTCTTC AGACAAGGAG GACGACGAAG CCACGGATTA CGTGTTCCGC 
ATCATCTACC CTGGCACGCA GTCTGAATTC GTGCCCCAGG ACCTAATGGA TGTCTCTATG 
AACAACCTTC CACCCCTATG GCAACCCAGT CCTCGGAAGT CCTCCTGCTC TTCCTGTTCA 
CAAAGCGGCT CAGCTGACGG CGGCTCAACC AATGGCTGTA ACCATGAGAG GGCCCCACTG 
AAACTGCTGT GTGATAACAT GAAGTACCAG ATCCTCTCCA GAGCCTTCTA TGGATGGCTC 
GCCTACTGCA GACACCTGTC CACCGTGAGG ACCCACCTGT CAGCCCTGGT CAATCACATG 
ATCGTGTCTC CAGACCTGCC CTGTGACGCT GGGCAAGGGT TGACAGCCAG CATCTGGGAA 
AAATACATCC AGGACAGCAC GACCTACCCG GAGCAGGAGC TGCTGCGTCT CATCTACTAT 
GGAGGTGTCC AGCCTGAGAT CCGCAGGGCA GTGTGGCCCT TCCTCCTCGG CCACTACCAG 
TTTGGGATGA CAGAGATGGA GAGGAAAGAG GTAGACGAAC AGATCCATGC CTGCTACGCA 
CAAACCATGT CGGAGTGGCT GGGCTGTGAA GCTATCGTGA GACAGAGGGA GCGGGAGTCC 
CACGCGGCTG CCCTGGCCAA GTGCTCTTCA GGAGCCAGCC TGGACAGCCA CCTTCACCGG 
ATGCTGCACC GGGACTCCAC CATCAGCAAT GAGTCATCCC AGAGCTGCAG CTCAGGCCGC 
CAGAACCTCC GACTGCAGAG CGACTCCAGC AGCAGCACAC AGGTATTTGA GTCTGTGGAT 
GAGGTGGAGC AGACAGAGGC AGAAGGCAGG TCAGAAGAAA AACATCCCAA AATCCCCAAT 
GGGAACCCAG CCAACGGCAC TTGCTCCCCA GACTCCGGAC ACCCTTCCTC CCACAACTTC 
TCCTCTGGTC TCTCCGAGCA CTCTGAGCCC AGTCTTAGCA CTGAAGACAG CGTCTTGGAT 
GCCCAACGCA GCCTTCCAGC TGTGTTTCGG CCTGGGGACA GCAGCGTGGA AGATGGGCAG 
AGCAGCGAAG CCACCACATC CCGGGACGAG GCCCCTCGGG AGGAGCTGGC CGTGCAAGAT 
AGCCTTGAGA GTGACCTCCT TGCCAACGAG AGCTTGGAGG AGTTCATGTC TATCCCTGGC 
AGCCTGGACG TGGCCCTACC TGAGAAGGAT GGCGCGGTGA TGGACGGCTG GCCCGGTGAG 
GCGGACAAGC CCAGTCGGGC TGACAGTGAG GACAACCTCT CAGAAGAGCC TGAGATGGAG 
AGCCTGTTCC CTGCCCTGGC TTCTCTGGCT GTGACCTCCT CTGCCAATAA CGAGGCATCC 
CCTGTGTCCT CCAGTGGAGT CACCTATTCT CCAGAACTAC TGGACCTGTA CACCGTGAAC 
CTGCACCGCA TTGAGAAAGA TGTGCAGAGG TGTGACCGCA GCTACTGGTA CTTCACAGCA 
GCCAACCTGG AGAAGCTGAG GAACATCATG TGCAGCTATA TCTGGCAGCA CATCGAGATC 
GGCTACGTCC AGGGCATGTG TGACCTCCTG GCCCCGTTGC TGGTCATCCT GGATGATGAC 
CAGGTTTCTC TGTATAGCCC CAGCTGTCCT GGAACTCAAG TTGTAGACCA GGCTGGCCTC 
GAACTCAGAA ATCCGCCTGC CTTTGCCTTC CCAATGCTGG GATTAGAGGC ATGCGCCACC 
ACCGCCCGGC TAGATAATTG GAATGTTAGC AGCTCACTTG GCGATACCAG TCCTCACCGG 
CATCTCACAT TTACCCTGAT GTCTACGTCA GACAGAATCA TTCCAGGGAA ATGTCAGACT 
AGTACCTCTC ATGAATATAA AAGCAAAACG TCTCAAAAAA AAATCAACAA ACTCAATCCA 
AGAAGATATA AAATATTACA CTGCATGGCC AAGCGGGTCT TATCCCAGAA ACGCAAGGGT 
GGTTCAATAT CCAAGCATCA ATCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sgsm1 ( XM_006535122.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535185.1 Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant X1, mRNA.

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CloneID	OMu35597
Clone ID Related Accession (Same CDS sequence)	XM_006535122.4 , XM_006535122.3
Accession Version	XM_006535122.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	small G protein signaling modulator 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535122.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG TCCCTGCGGA GGCCGAGACC CGCCAGAGGC TGCTGCGCAC TGTGAAGAAG 
GAGGTGAAGC AGATCATGGA AGAAGCCGTT ACCAGAAAGT TCGTCCACGA GGACAGCAGC 
CACATCATCT CCTTCTGTGC GGCCGTGGAG GCCTGCGTGC TGCACGGGCT GCGGCGGAGG 
GCGGCCGGCT TCCTGCGAAG CAACAAGATC GCGGCGCTCT TCATGAAGGT GGGCAAAGGC 
TTCCCTCCGG CCGAGGAGCT GAGCCGCAAG GTCCAGGAAC TGGAGCAACT CATTGAGAGC 
GCGCGAAACC AGATCCAGGG CCTGCAAGAG AACGTGCGGA AGCTGCCGAA GCTGCCTAAC 
CTGTCCCCGC TGGCCATCAA GCACCTGTGG ATCCGCACCG CCCTGTTCGA GAGGGTCCTG 
GACAAAATCG TCCACTACCT GGTGGAAAAC AGCAGTAAAT ACTACGAGAA GGAGGCACTC 
TTGATGGACC CGGTGGACGG ACCCATCCTG GCCTCTTTGT TGGTGGGGCC ATGTGCCCTG 
GAGTATACCA AGATGAAGAC TGCAGATCAC TTCTGGACTG ACCCCTCAGC CGACGAGCTG 
GTCCAGAGAC ACCGGATCCA CAGCTCACAT CTGAGACAGG ACTCGCCCAC CAAGCGGCCA 
GCGCTCTGTA TCCAGAAGAG GCATTCCAGT GGCAGCATGG ACGACCGGCC ATCTATCTCT 
GCCCGGGACT ATGTAGAATC TCTGCACCAG AACTCCAGGG CCACCCTGCT CTATGGAAAG 
AACAATGTTC TGGTTCAGCC AAGGGATGAC ATGGAGGCCG TGCCAGGGTA CCTGTCTCTG 
CACCAGACAG CGGATGTCAT GACCTTGAAG TGGACACCCA ATCAGCTGAT GAACGGGTCT 
GTGGGGGATC TGGACTACGA GAAGAGTGTC TACTGGGACT ACGCTGTGAC CATCCGCTTA 
GAGGAGATAG TTTACCTGCA CTGCCATCAA CAAGTGGACA GCGGCGGGAC TGTCGTGCTG 
GTGAGCCAGG ATGGAATCCA GAGGCCACCC TTCCGCTTCC CCAAGGGCGG GCACCTGCTA 
CAGTTCCTCT CCTGTCTGGA GAATGGGCTG CTTCCGCACG GGCAGCTGGA CCCGCCGCTT 
TGGTCACAGC GAGGAAAGGG GAAGGTATTT CCTAAGTTGC GCAAGCGCAG CCCACAGGGG 
TCCTCCGAGT CCACGTCTTC AGACAAGGAG GACGACGAAG CCACGGATTA CGTGTTCCGC 
ATCATCTACC CTGGCACGCA GTCTGAATTC GTTGCCCTTG ACCTCATGGG CAGCCCCCGG 
CCTGTGTCCA TTGGCCCTGC CTGGATGATG CTTGCTGCGG GCCAGTCCAT GCTAGTGGTG 
GCCAAGGGGC TCCAGTGGGC ACAAACCAGA GGGTGCCTCA CCATCCCCAC AAGGAGTGTG 
AAGGAGCAGC CCCCCATGCC CCAGGACCTA ATGGATGTCT CTATGAACAA CCTTCCACCC 
CTATGGCAAC CCAGTCCTCG GAAGTCCTCC TGCTCTTCCT GTTCACAAAG CGGCTCAGCT 
GACGGCGGCT CAACCAATGG CTGTAACCAT GAGAGGGCCC CACTGAAACT GCTGTGTGAT 
AACATGAAGT ACCAGATCCT CTCCAGAGCC TTCTATGGAT GGCTCGCCTA CTGCAGACAC 
CTGTCCACCG TGAGGACCCA CCTGTCAGCC CTGGTCAATC ACATGATCGT GTCTCCAGAC 
CTGCCCTGTG ACGCTGGGCA AGGGTTGACA GCCAGCATCT GGGAAAAATA CATCCAGGAC 
AGCACGACCT ACCCGGAGCA GGAGCTGCTG CGTCTCATCT ACTATGGAGG TGTCCAGCCT 
GAGATCCGCA GGGCAGTGTG GCCCTTCCTC CTCGGCCACT ACCAGTTTGG GATGACAGAG 
ATGGAGAGGA AAGAGGTAGA CGAACAGATC CATGCCTGCT ACGCACAAAC CATGTCGGAG 
TGGCTGGGCT GTGAAGCTAT CGTGAGACAG AGGGAGCGGG AGTCCCACGC GGCTGCCCTG 
GCCAAGTGCT CTTCAGGAGC CAGCCTGGAC AGCCACCTTC ACCGGATGCT GCACCGGGAC 
TCCACCATCA GCAATGAGTC ATCCCAGAGC TGCAGCTCAG GCCGCCAGAA CCTCCGACTG 
CAGAGCGACT CCAGCAGCAG CACACAGGTA TTTGAGTCTG TGGATGAGGT GGAGCAGACA 
GAGGCAGAAG GCAGGTCAGA AGAAAAACAT CCCAAAATCC CCAATGGGAA CCCAGCCAAC 
GGCACTTGCT CCCCAGACTC CGGACACCCT TCCTCCCACA ACTTCTCCTC TGGTCTCTCC 
GAGCACTCTG AGCCCAGTCT TAGCACTGAA GACAGCGTCT TGGATGCCCA ACGCAGCCTT 
CCAGCTGTGT TTCGGCCTGG GGACAGCAGC GTGGAAGATG GGCAGAGCAG CGAAGCCACC 
ACATCCCGGG ACGAGGCCCC TCGGGAGGAG CTGGCCGTGC AAGATAGCCT TGAGAGTGAC 
CTCCTTGCCA ACGAGAGCTT GGAGGAGTTC ATGTCTATCC CTGGCAGCCT GGACGTGGCC 
CTACCTGAGA AGGATGGCGC GGTGATGGAC GGCTGGCCCG GTGAGGCGGA CAAGCCCAGT 
CGGGCTGACA GTGAGGACAA CCTCTCAGAA GAGCCTGAGA TGGAGAGCCT GTTCCCTGCC 
CTGGCTTCTC TGGCTGTGAC CTCCTCTGCC AATAACGAGG CATCCCCTGT GTCCTCCAGT 
GGAGTCACCT ATTCTCCAGA ACTACTGGAC CTGTACACCG TGAACCTGCA CCGCATTGAG 
AAAGATGTGC AGAGGTGTGA CCGCAGCTAC TGGTACTTCA CAGCAGCCAA CCTGGAGAAG 
CTGAGGAACA TCATGTGCAG CTATATCTGG CAGCACATCG AGATCGGCTA CGTCCAGGGC 
ATGTGTGACC TCCTGGCCCC GTTGCTGGTC ATCCTGGATG ATGAGGCCCT GGCCTTCAGT 
TGCTTCACGG AGCTGATGAA GAGGATGAAC CAAAACTTCC CCCATGGAGG CGCCATGGAT 
ACGCACTTCG CCAACATGAG GTCTCTGATC CAGATCCTGG ACTCCGAGCT CTTCGAGCTG 
ATGCATCAGA ACGGCGATTA CACCCACTTC TACTTTTGCT ACCGCTGGTT CCTGCTGGAT 
TTCAAACGAG AGCTGGTCTA TGATGACGTC TTCTCCGTGT GGGAAACTAT CTGGGCTGCT 
AAGCACGTGT CCTCCGCCCA CTACGTGCTG TTCATCGCGC TGGCGCTGGT GGAGGTCTAC 
AGAGACATCA TCCTGGAGAA CAATATGGAC TTCACTGACA TTATCAAGTT CTTCAACGAA 
ATGGCTGAGC GGCACAACGC CAAGCAGATT CTGCAGCTCG CCCGGGACCT CGTGCATAAG 
GTGCAGATTC TAATTGAGAA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535185.1
CDS	94..3540
Translation	MASVPAEAETRQRLLRTVKKEVKQIMEEAVTRKFVHEDSSHIISFCAAVEACVLHGLRRRAAGFLRSNKIAALFMKVGKGFPPAEELSRKVQELEQLIESARNQIQGLQENVRKLPKLPNLSPLAIKHLWIRTALFERVLDKIVHYLVENSSKYYEKEALLMDPVDGPILASLLVGPCALEYTKMKTADHFWTDPSADELVQRHRIHSSHLRQDSPTKRPALCIQKRHSSGSMDDRPSISARDYVESLHQNSRATLLYGKNNVLVQPRDDMEAVPGYLSLHQTADVMTLKWTPNQLMNGSVGDLDYEKSVYWDYAVTIRLEEIVYLHCHQQVDSGGTVVLVSQDGIQRPPFRFPKGGHLLQFLSCLENGLLPHGQLDPPLWSQRGKGKVFPKLRKRSPQGSSESTSSDKEDDEATDYVFRIIYPGTQSEFVALDLMGSPRPVSIGPAWMMLAAGQSMLVVAKGLQWAQTRGCLTIPTRSVKEQPPMPQDLMDVSMNNLPPLWQPSPRKSSCSSCSQSGSADGGSTNGCNHERAPLKLLCDNMKYQILSRAFYGWLAYCRHLSTVRTHLSALVNHMIVSPDLPCDAGQGLTASIWEKYIQDSTTYPEQELLRLIYYGGVQPEIRRAVWPFLLGHYQFGMTEMERKEVDEQIHACYAQTMSEWLGCEAIVRQRERESHAAALAKCSSGASLDSHLHRMLHRDSTISNESSQSCSSGRQNLRLQSDSSSSTQVFESVDEVEQTEAEGRSEEKHPKIPNGNPANGTCSPDSGHPSSHNFSSGLSEHSEPSLSTEDSVLDAQRSLPAVFRPGDSSVEDGQSSEATTSRDEAPREELAVQDSLESDLLANESLEEFMSIPGSLDVALPEKDGAVMDGWPGEADKPSRADSEDNLSEEPEMESLFPALASLAVTSSANNEASPVSSSGVTYSPELLDLYTVNLHRIEKDVQRCDRSYWYFTAANLEKLRNIMCSYIWQHIEIGYVQGMCDLLAPLLVILDDEALAFSCFTELMKRMNQNFPHGGAMDTHFANMRSLIQILDSELFELMHQNGDYTHFYFCYRWFLLDFKRELVYDDVFSVWETIWAAKHVSSAHYVLFIALALVEVYRDIILENNMDFTDIIKFFNEMAERHNAKQILQLARDLVHKVQILIENK

Target ORF information:

RefSeq Version	XM_006535122.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus small G protein signaling modulator 1 (Sgsm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535122.3

ORF Insert Sequence:

ATGGCTTCGG TCCCTGCGGA GGCCGAGACC CGCCAGAGGC TGCTGCGCAC TGTGAAGAAG 
GAGGTGAAGC AGATCATGGA AGAAGCCGTT ACCAGAAAGT TCGTCCACGA GGACAGCAGC 
CACATCATCT CCTTCTGTGC GGCCGTGGAG GCCTGCGTGC TGCACGGGCT GCGGCGGAGG 
GCGGCCGGCT TCCTGCGAAG CAACAAGATC GCGGCGCTCT TCATGAAGGT GGGCAAAGGC 
TTCCCTCCGG CCGAGGAGCT GAGCCGCAAG GTCCAGGAAC TGGAGCAACT CATTGAGAGC 
GCGCGAAACC AGATCCAGGG CCTGCAAGAG AACGTGCGGA AGCTGCCGAA GCTGCCTAAC 
CTGTCCCCGC TGGCCATCAA GCACCTGTGG ATCCGCACCG CCCTGTTCGA GAGGGTCCTG 
GACAAAATCG TCCACTACCT GGTGGAAAAC AGCAGTAAAT ACTACGAGAA GGAGGCACTC 
TTGATGGACC CGGTGGACGG ACCCATCCTG GCCTCTTTGT TGGTGGGGCC ATGTGCCCTG 
GAGTATACCA AGATGAAGAC TGCAGATCAC TTCTGGACTG ACCCCTCAGC CGACGAGCTG 
GTCCAGAGAC ACCGGATCCA CAGCTCACAT CTGAGACAGG ACTCGCCCAC CAAGCGGCCA 
GCGCTCTGTA TCCAGAAGAG GCATTCCAGT GGCAGCATGG ACGACCGGCC ATCTATCTCT 
GCCCGGGACT ATGTAGAATC TCTGCACCAG AACTCCAGGG CCACCCTGCT CTATGGAAAG 
AACAATGTTC TGGTTCAGCC AAGGGATGAC ATGGAGGCCG TGCCAGGGTA CCTGTCTCTG 
CACCAGACAG CGGATGTCAT GACCTTGAAG TGGACACCCA ATCAGCTGAT GAACGGGTCT 
GTGGGGGATC TGGACTACGA GAAGAGTGTC TACTGGGACT ACGCTGTGAC CATCCGCTTA 
GAGGAGATAG TTTACCTGCA CTGCCATCAA CAAGTGGACA GCGGCGGGAC TGTCGTGCTG 
GTGAGCCAGG ATGGAATCCA GAGGCCACCC TTCCGCTTCC CCAAGGGCGG GCACCTGCTA 
CAGTTCCTCT CCTGTCTGGA GAATGGGCTG CTTCCGCACG GGCAGCTGGA CCCGCCGCTT 
TGGTCACAGC GAGGAAAGGG GAAGGTATTT CCTAAGTTGC GCAAGCGCAG CCCACAGGGG 
TCCTCCGAGT CCACGTCTTC AGACAAGGAG GACGACGAAG CCACGGATTA CGTGTTCCGC 
ATCATCTACC CTGGCACGCA GTCTGAATTC GTTGCCCTTG ACCTCATGGG CAGCCCCCGG 
CCTGTGTCCA TTGGCCCTGC CTGGATGATG CTTGCTGCGG GCCAGTCCAT GCTAGTGGTG 
GCCAAGGGGC TCCAGTGGGC ACAAACCAGA GGGTGCCTCA CCATCCCCAC AAGGAGTGTG 
AAGGAGCAGC CCCCCATGCC CCAGGACCTA ATGGATGTCT CTATGAACAA CCTTCCACCC 
CTATGGCAAC CCAGTCCTCG GAAGTCCTCC TGCTCTTCCT GTTCACAAAG CGGCTCAGCT 
GACGGCGGCT CAACCAATGG CTGTAACCAT GAGAGGGCCC CACTGAAACT GCTGTGTGAT 
AACATGAAGT ACCAGATCCT CTCCAGAGCC TTCTATGGAT GGCTCGCCTA CTGCAGACAC 
CTGTCCACCG TGAGGACCCA CCTGTCAGCC CTGGTCAATC ACATGATCGT GTCTCCAGAC 
CTGCCCTGTG ACGCTGGGCA AGGGTTGACA GCCAGCATCT GGGAAAAATA CATCCAGGAC 
AGCACGACCT ACCCGGAGCA GGAGCTGCTG CGTCTCATCT ACTATGGAGG TGTCCAGCCT 
GAGATCCGCA GGGCAGTGTG GCCCTTCCTC CTCGGCCACT ACCAGTTTGG GATGACAGAG 
ATGGAGAGGA AAGAGGTAGA CGAACAGATC CATGCCTGCT ACGCACAAAC CATGTCGGAG 
TGGCTGGGCT GTGAAGCTAT CGTGAGACAG AGGGAGCGGG AGTCCCACGC GGCTGCCCTG 
GCCAAGTGCT CTTCAGGAGC CAGCCTGGAC AGCCACCTTC ACCGGATGCT GCACCGGGAC 
TCCACCATCA GCAATGAGTC ATCCCAGAGC TGCAGCTCAG GCCGCCAGAA CCTCCGACTG 
CAGAGCGACT CCAGCAGCAG CACACAGGTA TTTGAGTCTG TGGATGAGGT GGAGCAGACA 
GAGGCAGAAG GCAGGTCAGA AGAAAAACAT CCCAAAATCC CCAATGGGAA CCCAGCCAAC 
GGCACTTGCT CCCCAGACTC CGGACACCCT TCCTCCCACA ACTTCTCCTC TGGTCTCTCC 
GAGCACTCTG AGCCCAGTCT TAGCACTGAA GACAGCGTCT TGGATGCCCA ACGCAGCCTT 
CCAGCTGTGT TTCGGCCTGG GGACAGCAGC GTGGAAGATG GGCAGAGCAG CGAAGCCACC 
ACATCCCGGG ACGAGGCCCC TCGGGAGGAG CTGGCCGTGC AAGATAGCCT TGAGAGTGAC 
CTCCTTGCCA ACGAGAGCTT GGAGGAGTTC ATGTCTATCC CTGGCAGCCT GGACGTGGCC 
CTACCTGAGA AGGATGGCGC GGTGATGGAC GGCTGGCCCG GTGAGGCGGA CAAGCCCAGT 
CGGGCTGACA GTGAGGACAA CCTCTCAGAA GAGCCTGAGA TGGAGAGCCT GTTCCCTGCC 
CTGGCTTCTC TGGCTGTGAC CTCCTCTGCC AATAACGAGG CATCCCCTGT GTCCTCCAGT 
GGAGTCACCT ATTCTCCAGA ACTACTGGAC CTGTACACCG TGAACCTGCA CCGCATTGAG 
AAAGATGTGC AGAGGTGTGA CCGCAGCTAC TGGTACTTCA CAGCAGCCAA CCTGGAGAAG 
CTGAGGAACA TCATGTGCAG CTATATCTGG CAGCACATCG AGATCGGCTA CGTCCAGGGC 
ATGTGTGACC TCCTGGCCCC GTTGCTGGTC ATCCTGGATG ATGAGGCCCT GGCCTTCAGT 
TGCTTCACGG AGCTGATGAA GAGGATGAAC CAAAACTTCC CCCATGGAGG CGCCATGGAT 
ACGCACTTCG CCAACATGAG GTCTCTGATC CAGATCCTGG ACTCCGAGCT CTTCGAGCTG 
ATGCATCAGA ACGGCGATTA CACCCACTTC TACTTTTGCT ACCGCTGGTT CCTGCTGGAT 
TTCAAACGAG AGCTGGTCTA TGATGACGTC TTCTCCGTGT GGGAAACTAT CTGGGCTGCT 
AAGCACGTGT CCTCCGCCCA CTACGTGCTG TTCATCGCGC TGGCGCTGGT GGAGGTCTAC 
AGAGACATCA TCCTGGAGAA CAATATGGAC TTCACTGACA TTATCAAGTT CTTCAACGAA 
ATGGCTGAGC GGCACAACGC CAAGCAGATT CTGCAGCTCG CCCGGGACCT CGTGCATAAG 
GTGCAGATTC TAATTGAGAA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2.
Structure (London, England : 1993)22(10)1408-20(2014 Oct)
Zhang Z,Wang S,Shen T,Chen J,Ding J

Expression pattern of NuIP gene in adult mouse brain.
Brain research130242-53(2009 Dec)
Luo Y,Sarabi SA,Backman C,Shan L,Hoffer B,Federoff H

Identification of a novel nurr1-interacting protein.
The Journal of neuroscience : the official journal of the Society for Neuroscience28(37)9277-86(2008 Sep)
Luo Y,Xing F,Guiliano R,Federoff HJ

Identification of three novel proteins (SGSM1, 2, 3) which modulate small G protein (RAP and RAB)-mediated signaling pathway.
Genomics90(2)249-60(2007 Aug)
Yang H,Sasaki T,Minoshima S,Shimizu N

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Sgsm1 cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

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