Ddx56 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx56
Entrez Gene ID	52513
Full Name	DEAD (Asp-Glu-Ala-Asp) box polypeptide 56
Synonyms	2600001H07Rik,D11Ertd619e,Noh61
General protein information	Preferred Names DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 Names probable ATP-dependent RNA helicase DDX56 ATP-dependent 61 kDa nucleolar RNA helicase DEAD box protein 56 DEAD-box RNA helicase nucleolar RNA helicase homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 A1\|11 3.94 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_026538.3	NP_080814.1	probable ATP-dependent RNA helicase DDX56
XM_017314650.2	XP_017170139.1	probable ATP-dependent RNA helicase DDX56 isoform X1
XM_011243731.3	XP_011242033.1	probable ATP-dependent RNA helicase DDX56 isoform X2
XM_017314650.1	XP_017170139.1	probable ATP-dependent RNA helicase DDX56 isoform X1
XM_011243731.2	XP_011242033.1	probable ATP-dependent RNA helicase DDX56 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ddx56	NP_080814.1
Magnaporthe oryzae (rice blast fungus)	MGG_04993	XP_003712493.1
Arabidopsis thaliana (thale cress)	AT4G34910	NP_195217.1
Bos taurus (cattle)	DDX56	NP_001029501.1
Drosophila melanogaster (fruit fly)	Hlc	NP_523434.2
Saccharomyces cerevisiae (baker's yeast)	DBP9	NP_013378.1
Schizosaccharomyces pombe (fission yeast)	SPCC1494.06c	NP_588531.1
Rattus norvegicus (Norway rat)	Ddx56	NP_001004211.1
Danio rerio (zebrafish)	ddx56	NP_001003876.1
Homo sapiens (human)	DDX56	NP_061955.1
Canis lupus familiaris (dog)	DDX56	XP_005642242.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011147	XP_564375.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011145	XP_309500.4
Caenorhabditis elegans (roundworm)	CELE_C24H12.4	NP_740966.1
Eremothecium gossypii	AGOS_AGR278C	NP_986944.1
Oryza sativa (rice)	Os03g0728800	NP_001051149.1
Xenopus tropicalis (tropical clawed frog)	ddx56	NP_001120553.1
Pan troglodytes (chimpanzee)	DDX56	XP_001145021.1
	DDX56	XP_001089984.2
Kluyveromyces lactis	KLLA0C04576g	XP_452403.1
Neurospora crassa	NCU07070	XP_960335.1

Gene Ontology
Bibliography

Related articles in PubMed

Tagging genes with cassette-exchange sites.
Cobellis G, Nicolaus G, Iovino M, Romito A, Marra E, Barbarisi M, Sardiello M, Di Giorgio FP, Iovino N, Zollo M, Ballabio A, Cortese R
Nucleic acids research33(4)e44(2005 Mar)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Targeting of nonexpressed genes in embryonic stem cells via homologous recombination.
Johnson RS, Sheng M, Greenberg ME, Kolodner RD, Papaioannou VE, Spiegelman BM
Science (New York, N.Y.)245(4923)1234-6(1989 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ddx56 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx56 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69440	XM_011243731.3 Latest version!	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu02529	NM_026538.3 Latest version!	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$321.30 $459.00
OMu77976	XM_017314650.2 Latest version!	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu77976	XM_017314650.1	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu69440	XM_011243731.2	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx56 ( XM_011243731.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242033.1 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu69440
Clone ID Related Accession (Same CDS sequence)	XM_011243731.3 , XM_011243731.2
Accession Version	XM_011243731.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase DDX56 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG CTGTGAGAGG CCTTGTTCTT GTTCCTACCA AGGAGCTGGC ACGGCAGGCC 
CAGGCCATGA TCCAGCAACT GGCTGCCTAC TGTGCTCGGG ATGTGCGAGT GGCTAACGTC 
TCAGCTGCAG AAGACTCAGC TTCTCAGAGA GCTGTGTTGA TGGAGAAGCC AGATGTGGTG 
GTGGGAACCC CATCCCGAGT GTTAAGCCAC TTGCAGCAGA ACACCTTGAA ACTCCGGGAC 
TCCCTAGAGC TGCTGGTGGT AGATGAAGCT GACCTTCTTT TTTCCTTTGG CTTTGAGGAT 
GAACTCAAGA GTCTTCTCTG TCACTTGCCT CGGATTTACC AGGCTTTTCT CATGTCGGCC 
ACTTTCAATG AGGATGTACA AACTCTGAAG GAGCTGGTAC TGCATAACCC GGTTACCCTC 
AAGTTACAGG AGTCCCAGCT GCCGGGGCCA GACCAGCTCC AGCAATTTCA GGTGGTCTGT 
GAGACAGAAG AGGACAAGTT CTTGCTGCTG TATGCCCTGC TCAAGCTGTC ACTGATTCGG 
GGCAAAGCCT TGCTCTTTGT CAACACTCTA GAGAGGGGTT ACCGGCTCCG TCTTTTCCTG 
GAACAGTTCA GCATTCCTTC CTGCGTGCTC AACGGAGAAC TCCCACTGCG CTCCAGGTGC 
CACATCATCT CACAGTTCAA CCAAGGCTTG TATGATTGCG TCATAGCGAC AGATGCTGAA 
ATCTTGGGAC CCCAAGTCAA AGGCAAACGT CGAGGCCGCG GGTCCAAAGG AAACAAGGCC 
TCTGATCCAG AGTCAGGTGT GGCTCGGGGC ATAGACTTCC ACCACGTGTC TGCGGTCCTC 
AATTTTGACC TTCCTCCCAC CGCAGAGGCT TATGTCCATC GAGCTGGCAG GACAGCTCGA 
GCCAACAACC CAGGCATAGT TCTGACCTTT GTGTTGCCCG CGGAACAGCC TTTCTTGGGA 
AAGATTGAGG ACCTTCTGAG TGGAGAGGGC GAGGCCCCCA TCCTGCTTCC ATACCAGTTC 
CAGATGGAGG AGATTGAAAG CTTTCGTTAT CGCTGCAGGG ATGCCATGCG CTCAGTGACC 
AAGCAGGCCA TTCGAGAGGC GAGACTGAAG GAGATCAAGG AGGAGCTTCT GCACTCAGAG 
AAGCTCAAGA CATACTTTGA AGACAACCCC AGAGACCTTC AGCTACTTCG CCATGATCTG 
CCCTTGCACC CGGCAGTGGT GAAGCCTCAT TTGGGCCATG TCCCTGACTA TTTGGTTCCT 
GCTGCTCTTC GTGGCCTTGT ACATCCTCGA AAGAAACGGA GAAAGGTGCC CTTCTCTAGG 
AAGGCTAAGA AGGTGAAGGC CCAGAACCCA CTGCGTGACT TTAAGCACAG GGGAAAGAAA 
CCTAAGCCCG CAGCAAAGCC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242033.1
CDS	239..1645
Translation	MEQAVRGLVLVPTKELARQAQAMIQQLAAYCARDVRVANVSAAEDSASQRAVLMEKPDVVVGTPSRVLSHLQQNTLKLRDSLELLVVDEADLLFSFGFEDELKSLLCHLPRIYQAFLMSATFNEDVQTLKELVLHNPVTLKLQESQLPGPDQLQQFQVVCETEEDKFLLLYALLKLSLIRGKALLFVNTLERGYRLRLFLEQFSIPSCVLNGELPLRSRCHIISQFNQGLYDCVIATDAEILGPQVKGKRRGRGSKGNKASDPESGVARGIDFHHVSAVLNFDLPPTAEAYVHRAGRTARANNPGIVLTFVLPAEQPFLGKIEDLLSGEGEAPILLPYQFQMEEIESFRYRCRDAMRSVTKQAIREARLKEIKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPAVVKPHLGHVPDYLVPAALRGLVHPRKKRRKVPFSRKAKKVKAQNPLRDFKHRGKKPKPAAKPS

Target ORF information:

RefSeq Version	XM_011243731.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243731.2

ORF Insert Sequence:

ATGGAGCAGG CTGTGAGAGG CCTTGTTCTT GTTCCTACCA AGGAGCTGGC ACGGCAGGCC 
CAGGCCATGA TCCAGCAACT GGCTGCCTAC TGTGCTCGGG ATGTGCGAGT GGCTAACGTC 
TCAGCTGCAG AAGACTCAGC TTCTCAGAGA GCTGTGTTGA TGGAGAAGCC AGATGTGGTG 
GTGGGAACCC CATCCCGAGT GTTAAGCCAC TTGCAGCAGA ACACCTTGAA ACTCCGGGAC 
TCCCTAGAGC TGCTGGTGGT AGATGAAGCT GACCTTCTTT TTTCCTTTGG CTTTGAGGAT 
GAACTCAAGA GTCTTCTCTG TCACTTGCCT CGGATTTACC AGGCTTTTCT CATGTCGGCC 
ACTTTCAATG AGGATGTACA AACTCTGAAG GAGCTGGTAC TGCATAACCC GGTTACCCTC 
AAGTTACAGG AGTCCCAGCT GCCGGGGCCA GACCAGCTCC AGCAATTTCA GGTGGTCTGT 
GAGACAGAAG AGGACAAGTT CTTGCTGCTG TATGCCCTGC TCAAGCTGTC ACTGATTCGG 
GGCAAAGCCT TGCTCTTTGT CAACACTCTA GAGAGGGGTT ACCGGCTCCG TCTTTTCCTG 
GAACAGTTCA GCATTCCTTC CTGCGTGCTC AACGGAGAAC TCCCACTGCG CTCCAGGTGC 
CACATCATCT CACAGTTCAA CCAAGGCTTG TATGATTGCG TCATAGCGAC AGATGCTGAA 
ATCTTGGGAC CCCAAGTCAA AGGCAAACGT CGAGGCCGCG GGTCCAAAGG AAACAAGGCC 
TCTGATCCAG AGTCAGGTGT GGCTCGGGGC ATAGACTTCC ACCACGTGTC TGCGGTCCTC 
AATTTTGACC TTCCTCCCAC CGCAGAGGCT TATGTCCATC GAGCTGGCAG GACAGCTCGA 
GCCAACAACC CAGGCATAGT TCTGACCTTT GTGTTGCCCG CGGAACAGCC TTTCTTGGGA 
AAGATTGAGG ACCTTCTGAG TGGAGAGGGC GAGGCCCCCA TCCTGCTTCC ATACCAGTTC 
CAGATGGAGG AGATTGAAAG CTTTCGTTAT CGCTGCAGGG ATGCCATGCG CTCAGTGACC 
AAGCAGGCCA TTCGAGAGGC GAGACTGAAG GAGATCAAGG AGGAGCTTCT GCACTCAGAG 
AAGCTCAAGA CATACTTTGA AGACAACCCC AGAGACCTTC AGCTACTTCG CCATGATCTG 
CCCTTGCACC CGGCAGTGGT GAAGCCTCAT TTGGGCCATG TCCCTGACTA TTTGGTTCCT 
GCTGCTCTTC GTGGCCTTGT ACATCCTCGA AAGAAACGGA GAAAGGTGCC CTTCTCTAGG 
AAGGCTAAGA AGGTGAAGGC CCAGAACCCA CTGCGTGACT TTAAGCACAG GGGAAAGAAA 
CCTAAGCCCG CAGCAAAGCC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx56 ( XM_011243731.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242033.1 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu69440
Clone ID Related Accession (Same CDS sequence)	XM_011243731.3 , XM_011243731.2
Accession Version	XM_011243731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase DDX56 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011243731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG CTGTGAGAGG CCTTGTTCTT GTTCCTACCA AGGAGCTGGC ACGGCAGGCC 
CAGGCCATGA TCCAGCAACT GGCTGCCTAC TGTGCTCGGG ATGTGCGAGT GGCTAACGTC 
TCAGCTGCAG AAGACTCAGC TTCTCAGAGA GCTGTGTTGA TGGAGAAGCC AGATGTGGTG 
GTGGGAACCC CATCCCGAGT GTTAAGCCAC TTGCAGCAGA ACACCTTGAA ACTCCGGGAC 
TCCCTAGAGC TGCTGGTGGT AGATGAAGCT GACCTTCTTT TTTCCTTTGG CTTTGAGGAT 
GAACTCAAGA GTCTTCTCTG TCACTTGCCT CGGATTTACC AGGCTTTTCT CATGTCGGCC 
ACTTTCAATG AGGATGTACA AACTCTGAAG GAGCTGGTAC TGCATAACCC GGTTACCCTC 
AAGTTACAGG AGTCCCAGCT GCCGGGGCCA GACCAGCTCC AGCAATTTCA GGTGGTCTGT 
GAGACAGAAG AGGACAAGTT CTTGCTGCTG TATGCCCTGC TCAAGCTGTC ACTGATTCGG 
GGCAAAGCCT TGCTCTTTGT CAACACTCTA GAGAGGGGTT ACCGGCTCCG TCTTTTCCTG 
GAACAGTTCA GCATTCCTTC CTGCGTGCTC AACGGAGAAC TCCCACTGCG CTCCAGGTGC 
CACATCATCT CACAGTTCAA CCAAGGCTTG TATGATTGCG TCATAGCGAC AGATGCTGAA 
ATCTTGGGAC CCCAAGTCAA AGGCAAACGT CGAGGCCGCG GGTCCAAAGG AAACAAGGCC 
TCTGATCCAG AGTCAGGTGT GGCTCGGGGC ATAGACTTCC ACCACGTGTC TGCGGTCCTC 
AATTTTGACC TTCCTCCCAC CGCAGAGGCT TATGTCCATC GAGCTGGCAG GACAGCTCGA 
GCCAACAACC CAGGCATAGT TCTGACCTTT GTGTTGCCCG CGGAACAGCC TTTCTTGGGA 
AAGATTGAGG ACCTTCTGAG TGGAGAGGGC GAGGCCCCCA TCCTGCTTCC ATACCAGTTC 
CAGATGGAGG AGATTGAAAG CTTTCGTTAT CGCTGCAGGG ATGCCATGCG CTCAGTGACC 
AAGCAGGCCA TTCGAGAGGC GAGACTGAAG GAGATCAAGG AGGAGCTTCT GCACTCAGAG 
AAGCTCAAGA CATACTTTGA AGACAACCCC AGAGACCTTC AGCTACTTCG CCATGATCTG 
CCCTTGCACC CGGCAGTGGT GAAGCCTCAT TTGGGCCATG TCCCTGACTA TTTGGTTCCT 
GCTGCTCTTC GTGGCCTTGT ACATCCTCGA AAGAAACGGA GAAAGGTGCC CTTCTCTAGG 
AAGGCTAAGA AGGTGAAGGC CCAGAACCCA CTGCGTGACT TTAAGCACAG GGGAAAGAAA 
CCTAAGCCCG CAGCAAAGCC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242033.1
CDS	261..1667
Translation	MEQAVRGLVLVPTKELARQAQAMIQQLAAYCARDVRVANVSAAEDSASQRAVLMEKPDVVVGTPSRVLSHLQQNTLKLRDSLELLVVDEADLLFSFGFEDELKSLLCHLPRIYQAFLMSATFNEDVQTLKELVLHNPVTLKLQESQLPGPDQLQQFQVVCETEEDKFLLLYALLKLSLIRGKALLFVNTLERGYRLRLFLEQFSIPSCVLNGELPLRSRCHIISQFNQGLYDCVIATDAEILGPQVKGKRRGRGSKGNKASDPESGVARGIDFHHVSAVLNFDLPPTAEAYVHRAGRTARANNPGIVLTFVLPAEQPFLGKIEDLLSGEGEAPILLPYQFQMEEIESFRYRCRDAMRSVTKQAIREARLKEIKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPAVVKPHLGHVPDYLVPAALRGLVHPRKKRRKVPFSRKAKKVKAQNPLRDFKHRGKKPKPAAKPS

Target ORF information:

RefSeq Version	XM_011243731.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243731.3

ORF Insert Sequence:

ATGGAGCAGG CTGTGAGAGG CCTTGTTCTT GTTCCTACCA AGGAGCTGGC ACGGCAGGCC 
CAGGCCATGA TCCAGCAACT GGCTGCCTAC TGTGCTCGGG ATGTGCGAGT GGCTAACGTC 
TCAGCTGCAG AAGACTCAGC TTCTCAGAGA GCTGTGTTGA TGGAGAAGCC AGATGTGGTG 
GTGGGAACCC CATCCCGAGT GTTAAGCCAC TTGCAGCAGA ACACCTTGAA ACTCCGGGAC 
TCCCTAGAGC TGCTGGTGGT AGATGAAGCT GACCTTCTTT TTTCCTTTGG CTTTGAGGAT 
GAACTCAAGA GTCTTCTCTG TCACTTGCCT CGGATTTACC AGGCTTTTCT CATGTCGGCC 
ACTTTCAATG AGGATGTACA AACTCTGAAG GAGCTGGTAC TGCATAACCC GGTTACCCTC 
AAGTTACAGG AGTCCCAGCT GCCGGGGCCA GACCAGCTCC AGCAATTTCA GGTGGTCTGT 
GAGACAGAAG AGGACAAGTT CTTGCTGCTG TATGCCCTGC TCAAGCTGTC ACTGATTCGG 
GGCAAAGCCT TGCTCTTTGT CAACACTCTA GAGAGGGGTT ACCGGCTCCG TCTTTTCCTG 
GAACAGTTCA GCATTCCTTC CTGCGTGCTC AACGGAGAAC TCCCACTGCG CTCCAGGTGC 
CACATCATCT CACAGTTCAA CCAAGGCTTG TATGATTGCG TCATAGCGAC AGATGCTGAA 
ATCTTGGGAC CCCAAGTCAA AGGCAAACGT CGAGGCCGCG GGTCCAAAGG AAACAAGGCC 
TCTGATCCAG AGTCAGGTGT GGCTCGGGGC ATAGACTTCC ACCACGTGTC TGCGGTCCTC 
AATTTTGACC TTCCTCCCAC CGCAGAGGCT TATGTCCATC GAGCTGGCAG GACAGCTCGA 
GCCAACAACC CAGGCATAGT TCTGACCTTT GTGTTGCCCG CGGAACAGCC TTTCTTGGGA 
AAGATTGAGG ACCTTCTGAG TGGAGAGGGC GAGGCCCCCA TCCTGCTTCC ATACCAGTTC 
CAGATGGAGG AGATTGAAAG CTTTCGTTAT CGCTGCAGGG ATGCCATGCG CTCAGTGACC 
AAGCAGGCCA TTCGAGAGGC GAGACTGAAG GAGATCAAGG AGGAGCTTCT GCACTCAGAG 
AAGCTCAAGA CATACTTTGA AGACAACCCC AGAGACCTTC AGCTACTTCG CCATGATCTG 
CCCTTGCACC CGGCAGTGGT GAAGCCTCAT TTGGGCCATG TCCCTGACTA TTTGGTTCCT 
GCTGCTCTTC GTGGCCTTGT ACATCCTCGA AAGAAACGGA GAAAGGTGCC CTTCTCTAGG 
AAGGCTAAGA AGGTGAAGGC CCAGAACCCA CTGCGTGACT TTAAGCACAG GGGAAAGAAA 
CCTAAGCCCG CAGCAAAGCC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx56 ( NM_026538.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080814.1 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), mRNA.

Price & Availability↑

CloneID	OMu02529
Clone ID Related Accession (Same CDS sequence)	NM_026538.3
Accession Version	NM_026538.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase DDX56
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL607152.21. On Mar 21, 2007 this sequence version replaced NM_026538.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK076975.1, AK011136.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGACC AAGAAGCGCT GGGTTTTGAA CACATGGGCC TGGATCCCCG GCTCCTGCAG 
GCTGTCACCG ATTTGGGCTG GTCGCGACCT ACGTTGATCC AGGAAAAGGC CATTCCTCTG 
GCGCTGGAGG GGAAGGACCT CCTGGCCCGC GCCCGCACAG GCTCCGGGAA GACCGCAGCT 
TATGCTATTC CGATGCTTCA GTCGCTTCTC CACAAGAAGG CGACAGGTCC TGTAATGGAG 
CAGGCTGTGA GAGGCCTTGT TCTTGTTCCT ACCAAGGAGC TGGCACGGCA GGCCCAGGCC 
ATGATCCAGC AACTGGCTGC CTACTGTGCT CGGGATGTGC GAGTGGCTAA CGTCTCAGCT 
GCAGAAGACT CAGCTTCTCA GAGAGCTGTG TTGATGGAGA AGCCAGATGT GGTGGTGGGA 
ACCCCATCCC GAGTGTTAAG CCACTTGCAG CAGAACACCT TGAAACTCCG GGACTCCCTA 
GAGCTGCTGG TGGTAGATGA AGCTGACCTT CTTTTTTCCT TTGGCTTTGA GGATGAACTC 
AAGAGTCTTC TCTGTCACTT GCCTCGGATT TACCAGGCTT TTCTCATGTC GGCCACTTTC 
AATGAGGATG TACAAACTCT GAAGGAGCTG GTACTGCATA ACCCGGTTAC CCTCAAGTTA 
CAGGAGTCCC AGCTGCCGGG GCCAGACCAG CTCCAGCAAT TTCAGGTGGT CTGTGAGACA 
GAAGAGGACA AGTTCTTGCT GCTGTATGCC CTGCTCAAGC TGTCACTGAT TCGGGGCAAA 
GCCTTGCTCT TTGTCAACAC TCTAGAGAGG GGTTACCGGC TCCGTCTTTT CCTGGAACAG 
TTCAGCATTC CTTCCTGCGT GCTCAACGGA GAACTCCCAC TGCGCTCCAG GTGCCACATC 
ATCTCACAGT TCAACCAAGG CTTGTATGAT TGCGTCATAG CGACAGATGC TGAAATCTTG 
GGACCCCAAG TCAAAGGCAA ACGTCGAGGC CGCGGGTCCA AAGGAAACAA GGCCTCTGAT 
CCAGAGTCAG GTGTGGCTCG GGGCATAGAC TTCCACCACG TGTCTGCGGT CCTCAATTTT 
GACCTTCCTC CCACCGCAGA GGCTTATGTC CATCGAGCTG GCAGGACAGC TCGAGCCAAC 
AACCCAGGCA TAGTTCTGAC CTTTGTGTTG CCCGCGGAAC AGCCTTTCTT GGGAAAGATT 
GAGGACCTTC TGAGTGGAGA GGGCGAGGCC CCCATCCTGC TTCCATACCA GTTCCAGATG 
GAGGAGATTG AAAGCTTTCG TTATCGCTGC AGGGATGCCA TGCGCTCAGT GACCAAGCAG 
GCCATTCGAG AGGCGAGACT GAAGGAGATC AAGGAGGAGC TTCTGCACTC AGAGAAGCTC 
AAGACATACT TTGAAGACAA CCCCAGAGAC CTTCAGCTAC TTCGCCATGA TCTGCCCTTG 
CACCCGGCAG TGGTGAAGCC TCATTTGGGC CATGTCCCTG ACTATTTGGT TCCTGCTGCT 
CTTCGTGGCC TTGTACATCC TCGAAAGAAA CGGAGAAAGG TGCCCTTCTC TAGGAAGGCT 
AAGAAGGTGA AGGCCCAGAA CCCACTGCGT GACTTTAAGC ACAGGGGAAA GAAACCTAAG 
CCCGCAGCAA AGCCTTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080814.1
CDS	11..1651
Translation	MEDQEALGFEHMGLDPRLLQAVTDLGWSRPTLIQEKAIPLALEGKDLLARARTGSGKTAAYAIPMLQSLLHKKATGPVMEQAVRGLVLVPTKELARQAQAMIQQLAAYCARDVRVANVSAAEDSASQRAVLMEKPDVVVGTPSRVLSHLQQNTLKLRDSLELLVVDEADLLFSFGFEDELKSLLCHLPRIYQAFLMSATFNEDVQTLKELVLHNPVTLKLQESQLPGPDQLQQFQVVCETEEDKFLLLYALLKLSLIRGKALLFVNTLERGYRLRLFLEQFSIPSCVLNGELPLRSRCHIISQFNQGLYDCVIATDAEILGPQVKGKRRGRGSKGNKASDPESGVARGIDFHHVSAVLNFDLPPTAEAYVHRAGRTARANNPGIVLTFVLPAEQPFLGKIEDLLSGEGEAPILLPYQFQMEEIESFRYRCRDAMRSVTKQAIREARLKEIKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPAVVKPHLGHVPDYLVPAALRGLVHPRKKRRKVPFSRKAKKVKAQNPLRDFKHRGKKPKPAAKPS

Target ORF information:

RefSeq Version	NM_026538.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026538.3

ORF Insert Sequence:

ATGGAGGACC AAGAAGCGCT GGGTTTTGAA CACATGGGCC TGGATCCCCG GCTCCTGCAG 
GCTGTCACCG ATTTGGGCTG GTCGCGACCT ACGTTGATCC AGGAAAAGGC CATTCCTCTG 
GCGCTGGAGG GGAAGGACCT CCTGGCCCGC GCCCGCACAG GCTCCGGGAA GACCGCAGCT 
TATGCTATTC CGATGCTTCA GTCGCTTCTC CACAAGAAGG CGACAGGTCC TGTAATGGAG 
CAGGCTGTGA GAGGCCTTGT TCTTGTTCCT ACCAAGGAGC TGGCACGGCA GGCCCAGGCC 
ATGATCCAGC AACTGGCTGC CTACTGTGCT CGGGATGTGC GAGTGGCTAA CGTCTCAGCT 
GCAGAAGACT CAGCTTCTCA GAGAGCTGTG TTGATGGAGA AGCCAGATGT GGTGGTGGGA 
ACCCCATCCC GAGTGTTAAG CCACTTGCAG CAGAACACCT TGAAACTCCG GGACTCCCTA 
GAGCTGCTGG TGGTAGATGA AGCTGACCTT CTTTTTTCCT TTGGCTTTGA GGATGAACTC 
AAGAGTCTTC TCTGTCACTT GCCTCGGATT TACCAGGCTT TTCTCATGTC GGCCACTTTC 
AATGAGGATG TACAAACTCT GAAGGAGCTG GTACTGCATA ACCCGGTTAC CCTCAAGTTA 
CAGGAGTCCC AGCTGCCGGG GCCAGACCAG CTCCAGCAAT TTCAGGTGGT CTGTGAGACA 
GAAGAGGACA AGTTCTTGCT GCTGTATGCC CTGCTCAAGC TGTCACTGAT TCGGGGCAAA 
GCCTTGCTCT TTGTCAACAC TCTAGAGAGG GGTTACCGGC TCCGTCTTTT CCTGGAACAG 
TTCAGCATTC CTTCCTGCGT GCTCAACGGA GAACTCCCAC TGCGCTCCAG GTGCCACATC 
ATCTCACAGT TCAACCAAGG CTTGTATGAT TGCGTCATAG CGACAGATGC TGAAATCTTG 
GGACCCCAAG TCAAAGGCAA ACGTCGAGGC CGCGGGTCCA AAGGAAACAA GGCCTCTGAT 
CCAGAGTCAG GTGTGGCTCG GGGCATAGAC TTCCACCACG TGTCTGCGGT CCTCAATTTT 
GACCTTCCTC CCACCGCAGA GGCTTATGTC CATCGAGCTG GCAGGACAGC TCGAGCCAAC 
AACCCAGGCA TAGTTCTGAC CTTTGTGTTG CCCGCGGAAC AGCCTTTCTT GGGAAAGATT 
GAGGACCTTC TGAGTGGAGA GGGCGAGGCC CCCATCCTGC TTCCATACCA GTTCCAGATG 
GAGGAGATTG AAAGCTTTCG TTATCGCTGC AGGGATGCCA TGCGCTCAGT GACCAAGCAG 
GCCATTCGAG AGGCGAGACT GAAGGAGATC AAGGAGGAGC TTCTGCACTC AGAGAAGCTC 
AAGACATACT TTGAAGACAA CCCCAGAGAC CTTCAGCTAC TTCGCCATGA TCTGCCCTTG 
CACCCGGCAG TGGTGAAGCC TCATTTGGGC CATGTCCCTG ACTATTTGGT TCCTGCTGCT 
CTTCGTGGCC TTGTACATCC TCGAAAGAAA CGGAGAAAGG TGCCCTTCTC TAGGAAGGCT 
AAGAAGGTGA AGGCCCAGAA CCCACTGCGT GACTTTAAGC ACAGGGGAAA GAAACCTAAG 
CCCGCAGCAA AGCCTTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx56 ( XM_017314650.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170139.1 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), transcript variant X1, mRNA.

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CloneID	OMu77976
Clone ID Related Accession (Same CDS sequence)	XM_017314650.2 , XM_017314650.1
Accession Version	XM_017314650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase DDX56 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACC AAGAAGCGCT GGGTTTTGAA CACATGGGCC TGGATCCCCG GCTCCTGCAG 
GCTGTCACCG ATTTGGGCTG GTCGCGACCT ACGTTGATCC AGGAAAAGGC CATTCCTCTG 
GCGCTGGAGG GGAAGGACCT CCTGGCCCGC GCCCGCACAG GCTCCGGGAA GACCGCAGCT 
TATGCTATTC CGATGCTTCA GTCGCTTCTC CACAAGAAGG CGGAGCTGGC ACGGCAGGCC 
CAGGCCATGA TCCAGCAACT GGCTGCCTAC TGTGCTCGGG ATGTGCGAGT GGCTAACGTC 
TCAGCTGCAG AAGACTCAGC TTCTCAGAGA GCTGTGTTGA TGGAGAAGCC AGATGTGGTG 
GTGGGAACCC CATCCCGAGT GTTAAGCCAC TTGCAGCAGA ACACCTTGAA ACTCCGGGAC 
TCCCTAGAGC TGCTGGTGGT AGATGAAGCT GACCTTCTTT TTTCCTTTGG CTTTGAGGAT 
GAACTCAAGA GTCTTCTCTG TCACTTGCCT CGGATTTACC AGGCTTTTCT CATGTCGGCC 
ACTTTCAATG AGGATGTACA AACTCTGAAG GAGCTGGTAC TGCATAACCC GGTTACCCTC 
AAGTTACAGG AGTCCCAGCT GCCGGGGCCA GACCAGCTCC AGCAATTTCA GGTGGTCTGT 
GAGACAGAAG AGGACAAGTT CTTGCTGCTG TATGCCCTGC TCAAGCTGTC ACTGATTCGG 
GGCAAAGCCT TGCTCTTTGT CAACACTCTA GAGAGGGGTT ACCGGCTCCG TCTTTTCCTG 
GAACAGTTCA GCATTCCTTC CTGCGTGCTC AACGGAGAAC TCCCACTGCG CTCCAGGTGC 
CACATCATCT CACAGTTCAA CCAAGGCTTG TATGATTGCG TCATAGCGAC AGATGCTGAA 
ATCTTGGGAC CCCAAGTCAA AGGCAAACGT CGAGGCCGCG GGTCCAAAGG AAACAAGGCC 
TCTGATCCAG AGTCAGGTGT GGCTCGGGGC ATAGACTTCC ACCACGTGTC TGCGGTCCTC 
AATTTTGACC TTCCTCCCAC CGCAGAGGCT TATGTCCATC GAGCTGGCAG GACAGCTCGA 
GCCAACAACC CAGGCATAGT TCTGACCTTT GTGTTGCCCG CGGAACAGCC TTTCTTGGGA 
AAGATTGAGG ACCTTCTGAG TGGAGAGGGC GAGGCCCCCA TCCTGCTTCC ATACCAGTTC 
CAGATGGAGG AGATTGAAAG CTTTCGTTAT CGCTGCAGGG ATGCCATGCG CTCAGTGACC 
AAGCAGGCCA TTCGAGAGGC GAGACTGAAG GAGATCAAGG AGGAGCTTCT GCACTCAGAG 
AAGCTCAAGA CATACTTTGA AGACAACCCC AGAGACCTTC AGCTACTTCG CCATGATCTG 
CCCTTGCACC CGGCAGTGGT GAAGCCTCAT TTGGGCCATG TCCCTGACTA TTTGGTTCCT 
GCTGCTCTTC GTGGCCTTGT ACATCCTCGA AAGAAACGGA GAAAGGTGCC CTTCTCTAGG 
AAGGCTAAGA AGGTGAAGGC CCAGAACCCA CTGCGTGACT TTAAGCACAG GGGAAAGAAA 
CCTAAGCCCG CAGCAAAGCC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170139.1
CDS	2068..3654
Translation	MEDQEALGFEHMGLDPRLLQAVTDLGWSRPTLIQEKAIPLALEGKDLLARARTGSGKTAAYAIPMLQSLLHKKAELARQAQAMIQQLAAYCARDVRVANVSAAEDSASQRAVLMEKPDVVVGTPSRVLSHLQQNTLKLRDSLELLVVDEADLLFSFGFEDELKSLLCHLPRIYQAFLMSATFNEDVQTLKELVLHNPVTLKLQESQLPGPDQLQQFQVVCETEEDKFLLLYALLKLSLIRGKALLFVNTLERGYRLRLFLEQFSIPSCVLNGELPLRSRCHIISQFNQGLYDCVIATDAEILGPQVKGKRRGRGSKGNKASDPESGVARGIDFHHVSAVLNFDLPPTAEAYVHRAGRTARANNPGIVLTFVLPAEQPFLGKIEDLLSGEGEAPILLPYQFQMEEIESFRYRCRDAMRSVTKQAIREARLKEIKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPAVVKPHLGHVPDYLVPAALRGLVHPRKKRRKVPFSRKAKKVKAQNPLRDFKHRGKKPKPAAKPS

Target ORF information:

RefSeq Version	XM_017314650.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314650.2

ORF Insert Sequence:

ATGGAGGACC AAGAAGCGCT GGGTTTTGAA CACATGGGCC TGGATCCCCG GCTCCTGCAG 
GCTGTCACCG ATTTGGGCTG GTCGCGACCT ACGTTGATCC AGGAAAAGGC CATTCCTCTG 
GCGCTGGAGG GGAAGGACCT CCTGGCCCGC GCCCGCACAG GCTCCGGGAA GACCGCAGCT 
TATGCTATTC CGATGCTTCA GTCGCTTCTC CACAAGAAGG CGGAGCTGGC ACGGCAGGCC 
CAGGCCATGA TCCAGCAACT GGCTGCCTAC TGTGCTCGGG ATGTGCGAGT GGCTAACGTC 
TCAGCTGCAG AAGACTCAGC TTCTCAGAGA GCTGTGTTGA TGGAGAAGCC AGATGTGGTG 
GTGGGAACCC CATCCCGAGT GTTAAGCCAC TTGCAGCAGA ACACCTTGAA ACTCCGGGAC 
TCCCTAGAGC TGCTGGTGGT AGATGAAGCT GACCTTCTTT TTTCCTTTGG CTTTGAGGAT 
GAACTCAAGA GTCTTCTCTG TCACTTGCCT CGGATTTACC AGGCTTTTCT CATGTCGGCC 
ACTTTCAATG AGGATGTACA AACTCTGAAG GAGCTGGTAC TGCATAACCC GGTTACCCTC 
AAGTTACAGG AGTCCCAGCT GCCGGGGCCA GACCAGCTCC AGCAATTTCA GGTGGTCTGT 
GAGACAGAAG AGGACAAGTT CTTGCTGCTG TATGCCCTGC TCAAGCTGTC ACTGATTCGG 
GGCAAAGCCT TGCTCTTTGT CAACACTCTA GAGAGGGGTT ACCGGCTCCG TCTTTTCCTG 
GAACAGTTCA GCATTCCTTC CTGCGTGCTC AACGGAGAAC TCCCACTGCG CTCCAGGTGC 
CACATCATCT CACAGTTCAA CCAAGGCTTG TATGATTGCG TCATAGCGAC AGATGCTGAA 
ATCTTGGGAC CCCAAGTCAA AGGCAAACGT CGAGGCCGCG GGTCCAAAGG AAACAAGGCC 
TCTGATCCAG AGTCAGGTGT GGCTCGGGGC ATAGACTTCC ACCACGTGTC TGCGGTCCTC 
AATTTTGACC TTCCTCCCAC CGCAGAGGCT TATGTCCATC GAGCTGGCAG GACAGCTCGA 
GCCAACAACC CAGGCATAGT TCTGACCTTT GTGTTGCCCG CGGAACAGCC TTTCTTGGGA 
AAGATTGAGG ACCTTCTGAG TGGAGAGGGC GAGGCCCCCA TCCTGCTTCC ATACCAGTTC 
CAGATGGAGG AGATTGAAAG CTTTCGTTAT CGCTGCAGGG ATGCCATGCG CTCAGTGACC 
AAGCAGGCCA TTCGAGAGGC GAGACTGAAG GAGATCAAGG AGGAGCTTCT GCACTCAGAG 
AAGCTCAAGA CATACTTTGA AGACAACCCC AGAGACCTTC AGCTACTTCG CCATGATCTG 
CCCTTGCACC CGGCAGTGGT GAAGCCTCAT TTGGGCCATG TCCCTGACTA TTTGGTTCCT 
GCTGCTCTTC GTGGCCTTGT ACATCCTCGA AAGAAACGGA GAAAGGTGCC CTTCTCTAGG 
AAGGCTAAGA AGGTGAAGGC CCAGAACCCA CTGCGTGACT TTAAGCACAG GGGAAAGAAA 
CCTAAGCCCG CAGCAAAGCC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx56 ( XM_017314650.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170139.1 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), transcript variant X1, mRNA.

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CloneID	OMu77976
Clone ID Related Accession (Same CDS sequence)	XM_017314650.2 , XM_017314650.1
Accession Version	XM_017314650.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase DDX56 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACC AAGAAGCGCT GGGTTTTGAA CACATGGGCC TGGATCCCCG GCTCCTGCAG 
GCTGTCACCG ATTTGGGCTG GTCGCGACCT ACGTTGATCC AGGAAAAGGC CATTCCTCTG 
GCGCTGGAGG GGAAGGACCT CCTGGCCCGC GCCCGCACAG GCTCCGGGAA GACCGCAGCT 
TATGCTATTC CGATGCTTCA GTCGCTTCTC CACAAGAAGG CGGAGCTGGC ACGGCAGGCC 
CAGGCCATGA TCCAGCAACT GGCTGCCTAC TGTGCTCGGG ATGTGCGAGT GGCTAACGTC 
TCAGCTGCAG AAGACTCAGC TTCTCAGAGA GCTGTGTTGA TGGAGAAGCC AGATGTGGTG 
GTGGGAACCC CATCCCGAGT GTTAAGCCAC TTGCAGCAGA ACACCTTGAA ACTCCGGGAC 
TCCCTAGAGC TGCTGGTGGT AGATGAAGCT GACCTTCTTT TTTCCTTTGG CTTTGAGGAT 
GAACTCAAGA GTCTTCTCTG TCACTTGCCT CGGATTTACC AGGCTTTTCT CATGTCGGCC 
ACTTTCAATG AGGATGTACA AACTCTGAAG GAGCTGGTAC TGCATAACCC GGTTACCCTC 
AAGTTACAGG AGTCCCAGCT GCCGGGGCCA GACCAGCTCC AGCAATTTCA GGTGGTCTGT 
GAGACAGAAG AGGACAAGTT CTTGCTGCTG TATGCCCTGC TCAAGCTGTC ACTGATTCGG 
GGCAAAGCCT TGCTCTTTGT CAACACTCTA GAGAGGGGTT ACCGGCTCCG TCTTTTCCTG 
GAACAGTTCA GCATTCCTTC CTGCGTGCTC AACGGAGAAC TCCCACTGCG CTCCAGGTGC 
CACATCATCT CACAGTTCAA CCAAGGCTTG TATGATTGCG TCATAGCGAC AGATGCTGAA 
ATCTTGGGAC CCCAAGTCAA AGGCAAACGT CGAGGCCGCG GGTCCAAAGG AAACAAGGCC 
TCTGATCCAG AGTCAGGTGT GGCTCGGGGC ATAGACTTCC ACCACGTGTC TGCGGTCCTC 
AATTTTGACC TTCCTCCCAC CGCAGAGGCT TATGTCCATC GAGCTGGCAG GACAGCTCGA 
GCCAACAACC CAGGCATAGT TCTGACCTTT GTGTTGCCCG CGGAACAGCC TTTCTTGGGA 
AAGATTGAGG ACCTTCTGAG TGGAGAGGGC GAGGCCCCCA TCCTGCTTCC ATACCAGTTC 
CAGATGGAGG AGATTGAAAG CTTTCGTTAT CGCTGCAGGG ATGCCATGCG CTCAGTGACC 
AAGCAGGCCA TTCGAGAGGC GAGACTGAAG GAGATCAAGG AGGAGCTTCT GCACTCAGAG 
AAGCTCAAGA CATACTTTGA AGACAACCCC AGAGACCTTC AGCTACTTCG CCATGATCTG 
CCCTTGCACC CGGCAGTGGT GAAGCCTCAT TTGGGCCATG TCCCTGACTA TTTGGTTCCT 
GCTGCTCTTC GTGGCCTTGT ACATCCTCGA AAGAAACGGA GAAAGGTGCC CTTCTCTAGG 
AAGGCTAAGA AGGTGAAGGC CCAGAACCCA CTGCGTGACT TTAAGCACAG GGGAAAGAAA 
CCTAAGCCCG CAGCAAAGCC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170139.1
CDS	673..2259
Translation	MEDQEALGFEHMGLDPRLLQAVTDLGWSRPTLIQEKAIPLALEGKDLLARARTGSGKTAAYAIPMLQSLLHKKAELARQAQAMIQQLAAYCARDVRVANVSAAEDSASQRAVLMEKPDVVVGTPSRVLSHLQQNTLKLRDSLELLVVDEADLLFSFGFEDELKSLLCHLPRIYQAFLMSATFNEDVQTLKELVLHNPVTLKLQESQLPGPDQLQQFQVVCETEEDKFLLLYALLKLSLIRGKALLFVNTLERGYRLRLFLEQFSIPSCVLNGELPLRSRCHIISQFNQGLYDCVIATDAEILGPQVKGKRRGRGSKGNKASDPESGVARGIDFHHVSAVLNFDLPPTAEAYVHRAGRTARANNPGIVLTFVLPAEQPFLGKIEDLLSGEGEAPILLPYQFQMEEIESFRYRCRDAMRSVTKQAIREARLKEIKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPAVVKPHLGHVPDYLVPAALRGLVHPRKKRRKVPFSRKAKKVKAQNPLRDFKHRGKKPKPAAKPS

Target ORF information:

RefSeq Version	XM_017314650.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314650.1

ORF Insert Sequence:

ATGGAGGACC AAGAAGCGCT GGGTTTTGAA CACATGGGCC TGGATCCCCG GCTCCTGCAG 
GCTGTCACCG ATTTGGGCTG GTCGCGACCT ACGTTGATCC AGGAAAAGGC CATTCCTCTG 
GCGCTGGAGG GGAAGGACCT CCTGGCCCGC GCCCGCACAG GCTCCGGGAA GACCGCAGCT 
TATGCTATTC CGATGCTTCA GTCGCTTCTC CACAAGAAGG CGGAGCTGGC ACGGCAGGCC 
CAGGCCATGA TCCAGCAACT GGCTGCCTAC TGTGCTCGGG ATGTGCGAGT GGCTAACGTC 
TCAGCTGCAG AAGACTCAGC TTCTCAGAGA GCTGTGTTGA TGGAGAAGCC AGATGTGGTG 
GTGGGAACCC CATCCCGAGT GTTAAGCCAC TTGCAGCAGA ACACCTTGAA ACTCCGGGAC 
TCCCTAGAGC TGCTGGTGGT AGATGAAGCT GACCTTCTTT TTTCCTTTGG CTTTGAGGAT 
GAACTCAAGA GTCTTCTCTG TCACTTGCCT CGGATTTACC AGGCTTTTCT CATGTCGGCC 
ACTTTCAATG AGGATGTACA AACTCTGAAG GAGCTGGTAC TGCATAACCC GGTTACCCTC 
AAGTTACAGG AGTCCCAGCT GCCGGGGCCA GACCAGCTCC AGCAATTTCA GGTGGTCTGT 
GAGACAGAAG AGGACAAGTT CTTGCTGCTG TATGCCCTGC TCAAGCTGTC ACTGATTCGG 
GGCAAAGCCT TGCTCTTTGT CAACACTCTA GAGAGGGGTT ACCGGCTCCG TCTTTTCCTG 
GAACAGTTCA GCATTCCTTC CTGCGTGCTC AACGGAGAAC TCCCACTGCG CTCCAGGTGC 
CACATCATCT CACAGTTCAA CCAAGGCTTG TATGATTGCG TCATAGCGAC AGATGCTGAA 
ATCTTGGGAC CCCAAGTCAA AGGCAAACGT CGAGGCCGCG GGTCCAAAGG AAACAAGGCC 
TCTGATCCAG AGTCAGGTGT GGCTCGGGGC ATAGACTTCC ACCACGTGTC TGCGGTCCTC 
AATTTTGACC TTCCTCCCAC CGCAGAGGCT TATGTCCATC GAGCTGGCAG GACAGCTCGA 
GCCAACAACC CAGGCATAGT TCTGACCTTT GTGTTGCCCG CGGAACAGCC TTTCTTGGGA 
AAGATTGAGG ACCTTCTGAG TGGAGAGGGC GAGGCCCCCA TCCTGCTTCC ATACCAGTTC 
CAGATGGAGG AGATTGAAAG CTTTCGTTAT CGCTGCAGGG ATGCCATGCG CTCAGTGACC 
AAGCAGGCCA TTCGAGAGGC GAGACTGAAG GAGATCAAGG AGGAGCTTCT GCACTCAGAG 
AAGCTCAAGA CATACTTTGA AGACAACCCC AGAGACCTTC AGCTACTTCG CCATGATCTG 
CCCTTGCACC CGGCAGTGGT GAAGCCTCAT TTGGGCCATG TCCCTGACTA TTTGGTTCCT 
GCTGCTCTTC GTGGCCTTGT ACATCCTCGA AAGAAACGGA GAAAGGTGCC CTTCTCTAGG 
AAGGCTAAGA AGGTGAAGGC CCAGAACCCA CTGCGTGACT TTAAGCACAG GGGAAAGAAA 
CCTAAGCCCG CAGCAAAGCC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tagging genes with cassette-exchange sites.
Nucleic acids research33(4)e44(2005 Mar)
Cobellis G,Nicolaus G,Iovino M,Romito A,Marra E,Barbarisi M,Sardiello M,Di Giorgio FP,Iovino N,Zollo M,Ballabio A,Cortese R

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Targeting of nonexpressed genes in embryonic stem cells via homologous recombination.
Science (New York, N.Y.)245(4923)1234-6(1989 Sep)
Johnson RS,Sheng M,Greenberg ME,Kolodner RD,Papaioannou VE,Spiegelman BM

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Ddx56 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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