Tet1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006513867.3	XP_006513930.1	methylcytosine dioxygenase TET1 isoform X1
XM_011243508.2	XP_011241810.1	methylcytosine dioxygenase TET1 isoform X1
XM_017314027.1	XP_017169516.1	methylcytosine dioxygenase TET1 isoform X3
XM_011243509.2	XP_011241811.1	methylcytosine dioxygenase TET1 isoform X2
XM_006513867.4	XP_006513930.1	methylcytosine dioxygenase TET1 isoform X1
XM_017314027.2	XP_017169516.1	methylcytosine dioxygenase TET1 isoform X2
NM_001253857.2	NP_001240786.1	methylcytosine dioxygenase TET1 isoform 1
NM_027384.1	NP_081660.1	methylcytosine dioxygenase TET1 isoform 2
XM_006513869.3	XP_006513932.1	methylcytosine dioxygenase TET1 isoform X1
XM_030245175.1	XP_030101035.1	methylcytosine dioxygenase TET1 isoform X3
NM_001253857.1	NP_001240786.1	methylcytosine dioxygenase TET1 isoform 1
XM_030245174.1	XP_030101034.1	methylcytosine dioxygenase TET1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu77588	XM_030245174.1 Latest version!	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu77588	XM_017314027.2 Latest version!	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu85476	XM_030245175.1 Latest version!	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu44647	XM_006513867.4 Latest version!	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu20453	NM_027384.1 Latest version!	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu20533	NM_001253857.2 Latest version!	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu20533	XM_011243509.2 Latest version!	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu44647	XM_011243508.2 Latest version!	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu44647	XM_006513869.3 Latest version!	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu44647	XM_006513867.3	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu77588	XM_017314027.1	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu20533	NM_001253857.1	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

Tet1 ( XM_017314027.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169516.1 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu77588
Clone ID Related Accession (Same CDS sequence)	XM_017314027.1 , XM_017314027.2 , XM_030245174.1
Accession Version	XM_017314027.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methylcytosine dioxygenase TET1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG 
GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT 
TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT 
CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC 
ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT 
TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC 
GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC 
TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC 
GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT 
CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG 
GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC 
AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG 
GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT 
CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC 
GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG 
AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC 
ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT 
TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA 
CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA 
AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC 
ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG 
CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA 
CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG 
TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT 
CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC 
GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA 
AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT 
CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT 
AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC 
CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA 
GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT 
GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT 
GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT 
CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG 
CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT 
GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG 
GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT 
GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG 
TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA 
GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT 
CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA 
CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC 
GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC 
GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC 
TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC 
GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG 
AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT 
TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG 
GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC 
ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT 
GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC 
ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA 
GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG 
AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTA CTGCTGGTCT CAGAAGGAAG 
AGGATCTCTG CCAGCCCAAA GGGAGCCCCA GGTTCACACA ACACTAAGAG CTTTTCATCA 
GCCTCATCTA CTTCTCACCT AGTGAAAGAC GAATCTACAG ACTTCTGTCC CCTGCAGGCT 
TCCTCCGCAG AAACATCTAC CTGTACGTAC AGTAAAACAG CCTCAGGTGG GTTTGCAGAA 
ACAAGTAGTA TTCTCCACTG CACAATGCCT TCTGGAGCAC ACAGTGGTGC TAATGCAGCT 
GCTGGGGAAT GTACTGGAAC GGTGCAGCCT GCCGAGGTGG CTGCTCATCC TCACCAGTCT 
CTTCCCACAG CCGATTCTCC CGTTCATGCT GAGCCTCTCA CTAGTCCATC TGAGCAGCTA 
ACTTCTAACC AGTCAAACCA GCAGCTCCCT CTCCTCAGCA ATTCTCAGAA ACTGGCTTCC 
TGTCAGGTGG AAGATGAGCG GCACCCTGAA GCGGATGAGC CTCAGCACCC CGAGGACGAT 
AACTTGCCTC AACTTGATGA ATTCTGGTCA GACAGTGAGG AGATCTACGC CGATCCTTCC 
TTTGGTGGCG TGGCGATAGC ACCCATTCAC GGCTCGGTGC TCATTGAGTG CGCTCGGAAG 
GAGCTTCATG CTACCACCTC TTTGCGCTCC CCCAAACGAG GGGTCCCTTT TCGTGTGTCC 
CTTGTATTCT ACCAGCACAA AAGCCTAAAC AAGCCTAATC ATGGTTTTGA TATCAACAAA 
ATTAAGTGTA AATGCAAAAA AGTAACGAAA AAAAAGCCCG CAGACCGGGA GTGTCCTGAT 
GTATCCCCCG AAGCCAATTT ATCACACCAA ATTCCTTCTC GAGTTGCATC AACCTTAACC 
CGAGACAATG TTGTTACCGT GTCCCCATAC TCTCTCACTC ATGTTGCGGG ACCCTACAAT 
CGTTGGGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169516.1
CDS	1138..5349
Translation	MDCSRRGHGEEEQRLDLITHPLENVRKNAGGMTGIEVEKWAPNKKSHLAEGQVKGSCDANLTGVENPQPSEDDKQQTNPSPTFAQTIRNGMKNVHCLPTDTHLPLNKLNHEEFSKALGNNSSKLLTDPSNCKDAMSVTTSGGECDHLKGPRNTLLFQKPGLNCRSGAEPTIFNNHPNTHSAGSRPHPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSESSSPSKPEKDEEAHQKTASLLNSCKAILHSVRKDLQDPNVQGKGLHHDTVVFNGQNRTFKSPDSFATNQALIKSQGYPSSPTAEKKGAAGGRAPFDGFENSHPLPIESHNLENCSQVLSCDQNLSSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINAEVRNAESTPESLVAKNTKQKHSQEKRMVHQKPPSSTQTKPSVPSAKPKKAQKKARATPHANKRKKKPPARSSQENDQKKQEQLAIEYSKMHDIWMSSKFQRFGQSSPRSFPVLLRNIPVFNQILKPVTQSKTPSQHNELFPPINQIKFTRNPELAKEKVKVEPSDSLPTCQFKTESGGQTFAEPADNSQGQPMVSVNQEAHPLPQSPPSNQCANIMAGAAQTQFHLGAQENLVHQIPPPTLPGTSPDTLLPDPASILRKGKVLHFDGITVVTEKREAQTSSNGPLGPTTDSAQSEFKESIMDLLSKPAKNLIAGLKEQEAAPCDCDGGTQKEKGPYYTHLGAGPSVAAVRELMETRFGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKLICLVRERVDHHCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGIDPKTCGASFSFGCSWSMYFNGCKFGRSENPRKFRLAPNYPLHNYYKRITGMSSEGSDVKTGWIIPDRKTLISREEKQLEKNLQELATVLAPLYKQMAPVAYQNQVEYEEVAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPEDEQLHVLPLYRLADTDEFGSVEGMKAKIKSGAIQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGTAGLRRKRISASPKGAPGSHNTKSFSSASSTSHLVKDESTDFCPLQASSAETSTCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGECTGTVQPAEVAAHPHQSLPTADSPVHAEPLTSPSEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEPQHPEDDNLPQLDEFWSDSEEIYADPSFGGVAIAPIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFYQHKSLNKPNHGFDINKIKCKCKKVTKKKPADRECPDVSPEANLSHQIPSRVASTLTRDNVVTVSPYSLTHVAGPYNRWV

Target ORF information:

RefSeq Version	XM_017314027.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314027.1

ORF Insert Sequence:

ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG 
GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT 
TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT 
CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC 
ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT 
TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC 
GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC 
TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC 
GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT 
CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG 
GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC 
AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG 
GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT 
CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC 
GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG 
AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC 
ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT 
TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA 
CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA 
AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC 
ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG 
CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA 
CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG 
TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT 
CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC 
GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA 
AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT 
CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT 
AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC 
CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA 
GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT 
GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT 
GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT 
CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG 
CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT 
GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG 
GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT 
GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG 
TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA 
GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT 
CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA 
CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC 
GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC 
GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC 
TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC 
GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG 
AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT 
TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG 
GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC 
ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT 
GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC 
ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA 
GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG 
AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTA CTGCTGGTCT CAGAAGGAAG 
AGGATCTCTG CCAGCCCAAA GGGAGCCCCA GGTTCACACA ACACTAAGAG CTTTTCATCA 
GCCTCATCTA CTTCTCACCT AGTGAAAGAC GAATCTACAG ACTTCTGTCC CCTGCAGGCT 
TCCTCCGCAG AAACATCTAC CTGTACGTAC AGTAAAACAG CCTCAGGTGG GTTTGCAGAA 
ACAAGTAGTA TTCTCCACTG CACAATGCCT TCTGGAGCAC ACAGTGGTGC TAATGCAGCT 
GCTGGGGAAT GTACTGGAAC GGTGCAGCCT GCCGAGGTGG CTGCTCATCC TCACCAGTCT 
CTTCCCACAG CCGATTCTCC CGTTCATGCT GAGCCTCTCA CTAGTCCATC TGAGCAGCTA 
ACTTCTAACC AGTCAAACCA GCAGCTCCCT CTCCTCAGCA ATTCTCAGAA ACTGGCTTCC 
TGTCAGGTGG AAGATGAGCG GCACCCTGAA GCGGATGAGC CTCAGCACCC CGAGGACGAT 
AACTTGCCTC AACTTGATGA ATTCTGGTCA GACAGTGAGG AGATCTACGC CGATCCTTCC 
TTTGGTGGCG TGGCGATAGC ACCCATTCAC GGCTCGGTGC TCATTGAGTG CGCTCGGAAG 
GAGCTTCATG CTACCACCTC TTTGCGCTCC CCCAAACGAG GGGTCCCTTT TCGTGTGTCC 
CTTGTATTCT ACCAGCACAA AAGCCTAAAC AAGCCTAATC ATGGTTTTGA TATCAACAAA 
ATTAAGTGTA AATGCAAAAA AGTAACGAAA AAAAAGCCCG CAGACCGGGA GTGTCCTGAT 
GTATCCCCCG AAGCCAATTT ATCACACCAA ATTCCTTCTC GAGTTGCATC AACCTTAACC 
CGAGACAATG TTGTTACCGT GTCCCCATAC TCTCTCACTC ATGTTGCGGG ACCCTACAAT 
CGTTGGGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tet1 ( XM_017314027.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169516.1 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu77588
Clone ID Related Accession (Same CDS sequence)	XM_017314027.1 , XM_017314027.2 , XM_030245174.1
Accession Version	XM_017314027.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	methylcytosine dioxygenase TET1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314027.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG 
GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT 
TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT 
CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC 
ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT 
TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC 
GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC 
TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC 
GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT 
CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG 
GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC 
AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG 
GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT 
CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC 
GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG 
AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC 
ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT 
TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA 
CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA 
AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC 
ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG 
CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA 
CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG 
TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT 
CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC 
GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA 
AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT 
CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT 
AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC 
CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA 
GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT 
GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT 
GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT 
CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG 
CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT 
GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG 
GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT 
GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG 
TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA 
GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT 
CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA 
CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC 
GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC 
GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC 
TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC 
GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG 
AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT 
TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG 
GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC 
ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT 
GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC 
ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA 
GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG 
AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTA CTGCTGGTCT CAGAAGGAAG 
AGGATCTCTG CCAGCCCAAA GGGAGCCCCA GGTTCACACA ACACTAAGAG CTTTTCATCA 
GCCTCATCTA CTTCTCACCT AGTGAAAGAC GAATCTACAG ACTTCTGTCC CCTGCAGGCT 
TCCTCCGCAG AAACATCTAC CTGTACGTAC AGTAAAACAG CCTCAGGTGG GTTTGCAGAA 
ACAAGTAGTA TTCTCCACTG CACAATGCCT TCTGGAGCAC ACAGTGGTGC TAATGCAGCT 
GCTGGGGAAT GTACTGGAAC GGTGCAGCCT GCCGAGGTGG CTGCTCATCC TCACCAGTCT 
CTTCCCACAG CCGATTCTCC CGTTCATGCT GAGCCTCTCA CTAGTCCATC TGAGCAGCTA 
ACTTCTAACC AGTCAAACCA GCAGCTCCCT CTCCTCAGCA ATTCTCAGAA ACTGGCTTCC 
TGTCAGGTGG AAGATGAGCG GCACCCTGAA GCGGATGAGC CTCAGCACCC CGAGGACGAT 
AACTTGCCTC AACTTGATGA ATTCTGGTCA GACAGTGAGG AGATCTACGC CGATCCTTCC 
TTTGGTGGCG TGGCGATAGC ACCCATTCAC GGCTCGGTGC TCATTGAGTG CGCTCGGAAG 
GAGCTTCATG CTACCACCTC TTTGCGCTCC CCCAAACGAG GGGTCCCTTT TCGTGTGTCC 
CTTGTATTCT ACCAGCACAA AAGCCTAAAC AAGCCTAATC ATGGTTTTGA TATCAACAAA 
ATTAAGTGTA AATGCAAAAA AGTAACGAAA AAAAAGCCCG CAGACCGGGA GTGTCCTGAT 
GTATCCCCCG AAGCCAATTT ATCACACCAA ATTCCTTCTC GAGTTGCATC AACCTTAACC 
CGAGACAATG TTGTTACCGT GTCCCCATAC TCTCTCACTC ATGTTGCGGG ACCCTACAAT 
CGTTGGGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169516.1
CDS	3919..8130
Translation	MDCSRRGHGEEEQRLDLITHPLENVRKNAGGMTGIEVEKWAPNKKSHLAEGQVKGSCDANLTGVENPQPSEDDKQQTNPSPTFAQTIRNGMKNVHCLPTDTHLPLNKLNHEEFSKALGNNSSKLLTDPSNCKDAMSVTTSGGECDHLKGPRNTLLFQKPGLNCRSGAEPTIFNNHPNTHSAGSRPHPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSESSSPSKPEKDEEAHQKTASLLNSCKAILHSVRKDLQDPNVQGKGLHHDTVVFNGQNRTFKSPDSFATNQALIKSQGYPSSPTAEKKGAAGGRAPFDGFENSHPLPIESHNLENCSQVLSCDQNLSSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINAEVRNAESTPESLVAKNTKQKHSQEKRMVHQKPPSSTQTKPSVPSAKPKKAQKKARATPHANKRKKKPPARSSQENDQKKQEQLAIEYSKMHDIWMSSKFQRFGQSSPRSFPVLLRNIPVFNQILKPVTQSKTPSQHNELFPPINQIKFTRNPELAKEKVKVEPSDSLPTCQFKTESGGQTFAEPADNSQGQPMVSVNQEAHPLPQSPPSNQCANIMAGAAQTQFHLGAQENLVHQIPPPTLPGTSPDTLLPDPASILRKGKVLHFDGITVVTEKREAQTSSNGPLGPTTDSAQSEFKESIMDLLSKPAKNLIAGLKEQEAAPCDCDGGTQKEKGPYYTHLGAGPSVAAVRELMETRFGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKLICLVRERVDHHCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGIDPKTCGASFSFGCSWSMYFNGCKFGRSENPRKFRLAPNYPLHNYYKRITGMSSEGSDVKTGWIIPDRKTLISREEKQLEKNLQELATVLAPLYKQMAPVAYQNQVEYEEVAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPEDEQLHVLPLYRLADTDEFGSVEGMKAKIKSGAIQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGTAGLRRKRISASPKGAPGSHNTKSFSSASSTSHLVKDESTDFCPLQASSAETSTCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGECTGTVQPAEVAAHPHQSLPTADSPVHAEPLTSPSEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEPQHPEDDNLPQLDEFWSDSEEIYADPSFGGVAIAPIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFYQHKSLNKPNHGFDINKIKCKCKKVTKKKPADRECPDVSPEANLSHQIPSRVASTLTRDNVVTVSPYSLTHVAGPYNRWV

Target ORF information:

RefSeq Version	XM_017314027.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314027.2

ORF Insert Sequence:

ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG 
GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT 
TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT 
CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC 
ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT 
TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC 
GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC 
TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC 
GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT 
CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG 
GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC 
AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG 
GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT 
CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC 
GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG 
AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC 
ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT 
TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA 
CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA 
AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC 
ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG 
CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA 
CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG 
TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT 
CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC 
GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA 
AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT 
CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT 
AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC 
CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA 
GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT 
GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT 
GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT 
CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG 
CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT 
GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG 
GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT 
GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG 
TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA 
GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT 
CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA 
CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC 
GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC 
GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC 
TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC 
GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG 
AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT 
TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG 
GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC 
ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT 
GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC 
ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA 
GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG 
AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTA CTGCTGGTCT CAGAAGGAAG 
AGGATCTCTG CCAGCCCAAA GGGAGCCCCA GGTTCACACA ACACTAAGAG CTTTTCATCA 
GCCTCATCTA CTTCTCACCT AGTGAAAGAC GAATCTACAG ACTTCTGTCC CCTGCAGGCT 
TCCTCCGCAG AAACATCTAC CTGTACGTAC AGTAAAACAG CCTCAGGTGG GTTTGCAGAA 
ACAAGTAGTA TTCTCCACTG CACAATGCCT TCTGGAGCAC ACAGTGGTGC TAATGCAGCT 
GCTGGGGAAT GTACTGGAAC GGTGCAGCCT GCCGAGGTGG CTGCTCATCC TCACCAGTCT 
CTTCCCACAG CCGATTCTCC CGTTCATGCT GAGCCTCTCA CTAGTCCATC TGAGCAGCTA 
ACTTCTAACC AGTCAAACCA GCAGCTCCCT CTCCTCAGCA ATTCTCAGAA ACTGGCTTCC 
TGTCAGGTGG AAGATGAGCG GCACCCTGAA GCGGATGAGC CTCAGCACCC CGAGGACGAT 
AACTTGCCTC AACTTGATGA ATTCTGGTCA GACAGTGAGG AGATCTACGC CGATCCTTCC 
TTTGGTGGCG TGGCGATAGC ACCCATTCAC GGCTCGGTGC TCATTGAGTG CGCTCGGAAG 
GAGCTTCATG CTACCACCTC TTTGCGCTCC CCCAAACGAG GGGTCCCTTT TCGTGTGTCC 
CTTGTATTCT ACCAGCACAA AAGCCTAAAC AAGCCTAATC ATGGTTTTGA TATCAACAAA 
ATTAAGTGTA AATGCAAAAA AGTAACGAAA AAAAAGCCCG CAGACCGGGA GTGTCCTGAT 
GTATCCCCCG AAGCCAATTT ATCACACCAA ATTCCTTCTC GAGTTGCATC AACCTTAACC 
CGAGACAATG TTGTTACCGT GTCCCCATAC TCTCTCACTC ATGTTGCGGG ACCCTACAAT 
CGTTGGGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tet1 ( XM_030245174.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101034.1 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X3, mRNA.

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CloneID	OMu77588
Clone ID Related Accession (Same CDS sequence)	XM_017314027.1 , XM_017314027.2 , XM_030245174.1
Accession Version	XM_030245174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	methylcytosine dioxygenase TET1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG 
GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT 
TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT 
CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC 
ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT 
TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC 
GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC 
TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC 
GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT 
CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG 
GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC 
AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG 
GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT 
CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC 
GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG 
AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC 
ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT 
TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA 
CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA 
AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC 
ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG 
CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA 
CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG 
TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT 
CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC 
GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA 
AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT 
CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT 
AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC 
CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA 
GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT 
GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT 
GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT 
CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG 
CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT 
GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG 
GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT 
GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG 
TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA 
GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT 
CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA 
CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC 
GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC 
GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC 
TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC 
GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG 
AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT 
TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG 
GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC 
ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT 
GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC 
ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA 
GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG 
AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTA CTGCTGGTCT CAGAAGGAAG 
AGGATCTCTG CCAGCCCAAA GGGAGCCCCA GGTTCACACA ACACTAAGAG CTTTTCATCA 
GCCTCATCTA CTTCTCACCT AGTGAAAGAC GAATCTACAG ACTTCTGTCC CCTGCAGGCT 
TCCTCCGCAG AAACATCTAC CTGTACGTAC AGTAAAACAG CCTCAGGTGG GTTTGCAGAA 
ACAAGTAGTA TTCTCCACTG CACAATGCCT TCTGGAGCAC ACAGTGGTGC TAATGCAGCT 
GCTGGGGAAT GTACTGGAAC GGTGCAGCCT GCCGAGGTGG CTGCTCATCC TCACCAGTCT 
CTTCCCACAG CCGATTCTCC CGTTCATGCT GAGCCTCTCA CTAGTCCATC TGAGCAGCTA 
ACTTCTAACC AGTCAAACCA GCAGCTCCCT CTCCTCAGCA ATTCTCAGAA ACTGGCTTCC 
TGTCAGGTGG AAGATGAGCG GCACCCTGAA GCGGATGAGC CTCAGCACCC CGAGGACGAT 
AACTTGCCTC AACTTGATGA ATTCTGGTCA GACAGTGAGG AGATCTACGC CGATCCTTCC 
TTTGGTGGCG TGGCGATAGC ACCCATTCAC GGCTCGGTGC TCATTGAGTG CGCTCGGAAG 
GAGCTTCATG CTACCACCTC TTTGCGCTCC CCCAAACGAG GGGTCCCTTT TCGTGTGTCC 
CTTGTATTCT ACCAGCACAA AAGCCTAAAC AAGCCTAATC ATGGTTTTGA TATCAACAAA 
ATTAAGTGTA AATGCAAAAA AGTAACGAAA AAAAAGCCCG CAGACCGGGA GTGTCCTGAT 
GTATCCCCCG AAGCCAATTT ATCACACCAA ATTCCTTCTC GAGTTGCATC AACCTTAACC 
CGAGACAATG TTGTTACCGT GTCCCCATAC TCTCTCACTC ATGTTGCGGG ACCCTACAAT 
CGTTGGGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101034.1
CDS	3572..7783
Translation	MDCSRRGHGEEEQRLDLITHPLENVRKNAGGMTGIEVEKWAPNKKSHLAEGQVKGSCDANLTGVENPQPSEDDKQQTNPSPTFAQTIRNGMKNVHCLPTDTHLPLNKLNHEEFSKALGNNSSKLLTDPSNCKDAMSVTTSGGECDHLKGPRNTLLFQKPGLNCRSGAEPTIFNNHPNTHSAGSRPHPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSESSSPSKPEKDEEAHQKTASLLNSCKAILHSVRKDLQDPNVQGKGLHHDTVVFNGQNRTFKSPDSFATNQALIKSQGYPSSPTAEKKGAAGGRAPFDGFENSHPLPIESHNLENCSQVLSCDQNLSSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINAEVRNAESTPESLVAKNTKQKHSQEKRMVHQKPPSSTQTKPSVPSAKPKKAQKKARATPHANKRKKKPPARSSQENDQKKQEQLAIEYSKMHDIWMSSKFQRFGQSSPRSFPVLLRNIPVFNQILKPVTQSKTPSQHNELFPPINQIKFTRNPELAKEKVKVEPSDSLPTCQFKTESGGQTFAEPADNSQGQPMVSVNQEAHPLPQSPPSNQCANIMAGAAQTQFHLGAQENLVHQIPPPTLPGTSPDTLLPDPASILRKGKVLHFDGITVVTEKREAQTSSNGPLGPTTDSAQSEFKESIMDLLSKPAKNLIAGLKEQEAAPCDCDGGTQKEKGPYYTHLGAGPSVAAVRELMETRFGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKLICLVRERVDHHCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGIDPKTCGASFSFGCSWSMYFNGCKFGRSENPRKFRLAPNYPLHNYYKRITGMSSEGSDVKTGWIIPDRKTLISREEKQLEKNLQELATVLAPLYKQMAPVAYQNQVEYEEVAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPEDEQLHVLPLYRLADTDEFGSVEGMKAKIKSGAIQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGTAGLRRKRISASPKGAPGSHNTKSFSSASSTSHLVKDESTDFCPLQASSAETSTCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGECTGTVQPAEVAAHPHQSLPTADSPVHAEPLTSPSEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEPQHPEDDNLPQLDEFWSDSEEIYADPSFGGVAIAPIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFYQHKSLNKPNHGFDINKIKCKCKKVTKKKPADRECPDVSPEANLSHQIPSRVASTLTRDNVVTVSPYSLTHVAGPYNRWV

Target ORF information:

RefSeq Version	XM_030245174.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245174.1

ORF Insert Sequence:

ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG 
GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT 
TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT 
CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC 
ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT 
TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC 
GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC 
TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC 
GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT 
CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG 
GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC 
AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG 
GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT 
CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC 
GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG 
AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC 
ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT 
TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA 
CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA 
AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC 
ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG 
CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA 
CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG 
TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT 
CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC 
GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA 
AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT 
CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT 
AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC 
CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA 
GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT 
GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT 
GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT 
CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG 
CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT 
GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG 
GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT 
GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG 
TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA 
GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT 
CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA 
CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC 
GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC 
GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC 
TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC 
GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG 
AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT 
TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG 
GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC 
ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT 
GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC 
ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA 
GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG 
AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTA CTGCTGGTCT CAGAAGGAAG 
AGGATCTCTG CCAGCCCAAA GGGAGCCCCA GGTTCACACA ACACTAAGAG CTTTTCATCA 
GCCTCATCTA CTTCTCACCT AGTGAAAGAC GAATCTACAG ACTTCTGTCC CCTGCAGGCT 
TCCTCCGCAG AAACATCTAC CTGTACGTAC AGTAAAACAG CCTCAGGTGG GTTTGCAGAA 
ACAAGTAGTA TTCTCCACTG CACAATGCCT TCTGGAGCAC ACAGTGGTGC TAATGCAGCT 
GCTGGGGAAT GTACTGGAAC GGTGCAGCCT GCCGAGGTGG CTGCTCATCC TCACCAGTCT 
CTTCCCACAG CCGATTCTCC CGTTCATGCT GAGCCTCTCA CTAGTCCATC TGAGCAGCTA 
ACTTCTAACC AGTCAAACCA GCAGCTCCCT CTCCTCAGCA ATTCTCAGAA ACTGGCTTCC 
TGTCAGGTGG AAGATGAGCG GCACCCTGAA GCGGATGAGC CTCAGCACCC CGAGGACGAT 
AACTTGCCTC AACTTGATGA ATTCTGGTCA GACAGTGAGG AGATCTACGC CGATCCTTCC 
TTTGGTGGCG TGGCGATAGC ACCCATTCAC GGCTCGGTGC TCATTGAGTG CGCTCGGAAG 
GAGCTTCATG CTACCACCTC TTTGCGCTCC CCCAAACGAG GGGTCCCTTT TCGTGTGTCC 
CTTGTATTCT ACCAGCACAA AAGCCTAAAC AAGCCTAATC ATGGTTTTGA TATCAACAAA 
ATTAAGTGTA AATGCAAAAA AGTAACGAAA AAAAAGCCCG CAGACCGGGA GTGTCCTGAT 
GTATCCCCCG AAGCCAATTT ATCACACCAA ATTCCTTCTC GAGTTGCATC AACCTTAACC 
CGAGACAATG TTGTTACCGT GTCCCCATAC TCTCTCACTC ATGTTGCGGG ACCCTACAAT 
CGTTGGGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tet1 ( XM_030245175.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101035.1 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X5, mRNA.

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CloneID	OMu85476
Clone ID Related Accession (Same CDS sequence)	XM_030245175.1
Accession Version	XM_030245175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	methylcytosine dioxygenase TET1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG 
GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT 
TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT 
CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC 
ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT 
TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC 
GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC 
TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC 
GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT 
CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG 
GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC 
AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG 
GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT 
CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC 
GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG 
AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC 
ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT 
TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA 
CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA 
AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC 
ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG 
CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA 
CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG 
TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT 
CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC 
GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA 
AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT 
CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT 
AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC 
CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA 
GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT 
GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT 
GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT 
CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG 
CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT 
GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG 
GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT 
GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG 
TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA 
GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT 
CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA 
CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC 
GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC 
GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC 
TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC 
GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG 
AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT 
TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG 
GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC 
ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT 
GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC 
ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA 
GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG 
AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTT CACACAACAC TAAGAGCTTT 
TCATCAGCCT CATCTACTTC TCACCTAGTG AAAGACGAAT CTACAGACTT CTGTCCCCTG 
CAGGCTTCCT CCGCAGAAAC ATCTACCTGT ACGTACAGTA AAACAGCCTC AGGTGGGTTT 
GCAGAAACAA GTAGTATTCT CCACTGCACA ATGCCTTCTG GAGCACACAG TGGTGCTAAT 
GCAGCTGCTG GGGAATGTAC TGGAACGGTG CAGCCTGCCG AGGTGGCTGC TCATCCTCAC 
CAGTCTCTTC CCACAGCCGA TTCTCCCGTT CATGCTGAGC CTCTCACTAG TCCATCTGAG 
CAGCTAACTT CTAACCAGTC AAACCAGCAG CTCCCTCTCC TCAGCAATTC TCAGAAACTG 
GCTTCCTGTC AGGTGGAAGA TGAGCGGCAC CCTGAAGCGG ATGAGCCTCA GCACCCCGAG 
GACGATAACT TGCCTCAACT TGATGAATTC TGGTCAGACA GTGAGGAGAT CTACGCCGAT 
CCTTCCTTTG GTGGCGTGGC GATAGCACCC ATTCACGGCT CGGTGCTCAT TGAGTGCGCT 
CGGAAGGAGC TTCATGCTAC CACCTCTTTG CGCTCCCCCA AACGAGGGGT CCCTTTTCGT 
GTGTCCCTTG TATTCTACCA GCACAAAAGC CTAAACAAGC CTAATCATGG TTTTGATATC 
AACAAAATTA AGTGTAAATG CAAAAAAGTA ACGAAAAAAA AGCCCGCAGA CCGGGAGTGT 
CCTGATGTAT CCCCCGAAGC CAATTTATCA CACCAAATTC CTTCTCGAGT TGCATCAACC 
TTAACCCGAG ACAATGTTGT TACCGTGTCC CCATACTCTC TCACTCATGT TGCGGGACCC 
TACAATCGTT GGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101035.1
CDS	3919..8076
Translation	MDCSRRGHGEEEQRLDLITHPLENVRKNAGGMTGIEVEKWAPNKKSHLAEGQVKGSCDANLTGVENPQPSEDDKQQTNPSPTFAQTIRNGMKNVHCLPTDTHLPLNKLNHEEFSKALGNNSSKLLTDPSNCKDAMSVTTSGGECDHLKGPRNTLLFQKPGLNCRSGAEPTIFNNHPNTHSAGSRPHPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSESSSPSKPEKDEEAHQKTASLLNSCKAILHSVRKDLQDPNVQGKGLHHDTVVFNGQNRTFKSPDSFATNQALIKSQGYPSSPTAEKKGAAGGRAPFDGFENSHPLPIESHNLENCSQVLSCDQNLSSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINAEVRNAESTPESLVAKNTKQKHSQEKRMVHQKPPSSTQTKPSVPSAKPKKAQKKARATPHANKRKKKPPARSSQENDQKKQEQLAIEYSKMHDIWMSSKFQRFGQSSPRSFPVLLRNIPVFNQILKPVTQSKTPSQHNELFPPINQIKFTRNPELAKEKVKVEPSDSLPTCQFKTESGGQTFAEPADNSQGQPMVSVNQEAHPLPQSPPSNQCANIMAGAAQTQFHLGAQENLVHQIPPPTLPGTSPDTLLPDPASILRKGKVLHFDGITVVTEKREAQTSSNGPLGPTTDSAQSEFKESIMDLLSKPAKNLIAGLKEQEAAPCDCDGGTQKEKGPYYTHLGAGPSVAAVRELMETRFGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKLICLVRERVDHHCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGIDPKTCGASFSFGCSWSMYFNGCKFGRSENPRKFRLAPNYPLHNYYKRITGMSSEGSDVKTGWIIPDRKTLISREEKQLEKNLQELATVLAPLYKQMAPVAYQNQVEYEEVAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPEDEQLHVLPLYRLADTDEFGSVEGMKAKIKSGAIQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGSHNTKSFSSASSTSHLVKDESTDFCPLQASSAETSTCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGECTGTVQPAEVAAHPHQSLPTADSPVHAEPLTSPSEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEPQHPEDDNLPQLDEFWSDSEEIYADPSFGGVAIAPIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFYQHKSLNKPNHGFDINKIKCKCKKVTKKKPADRECPDVSPEANLSHQIPSRVASTLTRDNVVTVSPYSLTHVAGPYNRWV

Target ORF information:

RefSeq Version	XM_030245175.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245175.1

ORF Insert Sequence:

ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG 
GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT 
TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT 
CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC 
ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT 
TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC 
GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC 
TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC 
GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT 
CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG 
GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC 
AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG 
GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT 
CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC 
GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG 
AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC 
ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT 
TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA 
CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA 
AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC 
ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG 
CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA 
CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG 
TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT 
CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC 
GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA 
AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT 
CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT 
AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC 
CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA 
GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT 
GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT 
GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT 
CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG 
CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT 
GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG 
GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT 
GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG 
TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA 
GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT 
CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA 
CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC 
GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC 
GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC 
TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC 
GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG 
AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT 
TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG 
GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC 
ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT 
GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC 
ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA 
GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG 
AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTT CACACAACAC TAAGAGCTTT 
TCATCAGCCT CATCTACTTC TCACCTAGTG AAAGACGAAT CTACAGACTT CTGTCCCCTG 
CAGGCTTCCT CCGCAGAAAC ATCTACCTGT ACGTACAGTA AAACAGCCTC AGGTGGGTTT 
GCAGAAACAA GTAGTATTCT CCACTGCACA ATGCCTTCTG GAGCACACAG TGGTGCTAAT 
GCAGCTGCTG GGGAATGTAC TGGAACGGTG CAGCCTGCCG AGGTGGCTGC TCATCCTCAC 
CAGTCTCTTC CCACAGCCGA TTCTCCCGTT CATGCTGAGC CTCTCACTAG TCCATCTGAG 
CAGCTAACTT CTAACCAGTC AAACCAGCAG CTCCCTCTCC TCAGCAATTC TCAGAAACTG 
GCTTCCTGTC AGGTGGAAGA TGAGCGGCAC CCTGAAGCGG ATGAGCCTCA GCACCCCGAG 
GACGATAACT TGCCTCAACT TGATGAATTC TGGTCAGACA GTGAGGAGAT CTACGCCGAT 
CCTTCCTTTG GTGGCGTGGC GATAGCACCC ATTCACGGCT CGGTGCTCAT TGAGTGCGCT 
CGGAAGGAGC TTCATGCTAC CACCTCTTTG CGCTCCCCCA AACGAGGGGT CCCTTTTCGT 
GTGTCCCTTG TATTCTACCA GCACAAAAGC CTAAACAAGC CTAATCATGG TTTTGATATC 
AACAAAATTA AGTGTAAATG CAAAAAAGTA ACGAAAAAAA AGCCCGCAGA CCGGGAGTGT 
CCTGATGTAT CCCCCGAAGC CAATTTATCA CACCAAATTC CTTCTCGAGT TGCATCAACC 
TTAACCCGAG ACAATGTTGT TACCGTGTCC CCATACTCTC TCACTCATGT TGCGGGACCC 
TACAATCGTT GGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tet1 ( XM_006513867.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513930.1 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X1, mRNA.

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CloneID	OMu44647
Clone ID Related Accession (Same CDS sequence)	XM_006513867.3 , XM_011243508.2 , XM_006513867.4 , XM_006513869.3
Accession Version	XM_006513867.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	methylcytosine dioxygenase TET1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513867.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG 
AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC 
CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT 
ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC 
GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG 
GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG 
CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA 
AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC 
CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT 
ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC 
TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA 
ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT 
CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC 
CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG 
CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT 
GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA 
GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC 
TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC 
AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA 
AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT 
TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC 
GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513930.1
CDS	413..6586
Translation	MSRSRPAKPSKSVKTKLQKKKDIQMKTKTSKQAVRHGASAKAVNPGKPKQLIKRRDGKKETEDKTPTPAPSFLTRAGAARMNRDRNQVLFQNPDSLTCNGFTMALRRTSLSWRLSQRPVVTPKPKKVPPSKKQCTHNIQDEPGVKHSENDSVPSQHATVSPGTENGEQNRCLVEGESQEITQSCPVFEERIEDTQSCISASGNLEAEISWPLEGTHCEELLSHQTSDNECTSPQECAPLPQRSTSEVTSQKNTSNQLADLSSQVESIKLSDPSPNPTGSDHNGFPDSSFRIVPELDLKTCMPLDESVYPTALIRFILAGSQPDVFDTKPQEKTLITTPEQVGSHPNQVLDATSVLGQAFSTLPLQWGFSGANLVQVEALGKGSDSPEDLGAITMLNQQETVAMDMDRNATPDLPIFLPKPPNTVATYSSPLLGPEPHSSTSCGLEVQGATPILTLDSGHTPQLPPNPESSSVPLVIAANGTRAEKQFGTSLFPAVPQGFTVAAENEVQHAPLDLTQGSQAAPSKLEGEISRVSITGSADVKATAMSMPVTQASTSSPPCNSTPPMVERRKRKACGVCEPCQQKANCGECTYCKNRKNSHQICKKRKCEVLKKKPEATSQAQVTKENKRPQREKKPKVLKTDFNNKPVNGPKSESMDCSRRGHGEEEQRLDLITHPLENVRKNAGGMTGIEVEKWAPNKKSHLAEGQVKGSCDANLTGVENPQPSEDDKQQTNPSPTFAQTIRNGMKNVHCLPTDTHLPLNKLNHEEFSKALGNNSSKLLTDPSNCKDAMSVTTSGGECDHLKGPRNTLLFQKPGLNCRSGAEPTIFNNHPNTHSAGSRPHPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSESSSPSKPEKDEEAHQKTASLLNSCKAILHSVRKDLQDPNVQGKGLHHDTVVFNGQNRTFKSPDSFATNQALIKSQGYPSSPTAEKKGAAGGRAPFDGFENSHPLPIESHNLENCSQVLSCDQNLSSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINAEVRNAESTPESLVAKNTKQKHSQEKRMVHQKPPSSTQTKPSVPSAKPKKAQKKARATPHANKRKKKPPARSSQENDQKKQEQLAIEYSKMHDIWMSSKFQRFGQSSPRSFPVLLRNIPVFNQILKPVTQSKTPSQHNELFPPINQIKFTRNPELAKEKVKVEPSDSLPTCQFKTESGGQTFAEPADNSQGQPMVSVNQEAHPLPQSPPSNQCANIMAGAAQTQFHLGAQENLVHQIPPPTLPGTSPDTLLPDPASILRKGKVLHFDGITVVTEKREAQTSSNGPLGPTTDSAQSEFKESIMDLLSKPAKNLIAGLKEQEAAPCDCDGGTQKEKGPYYTHLGAGPSVAAVRELMETRFGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKLICLVRERVDHHCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGIDPKTCGASFSFGCSWSMYFNGCKFGRSENPRKFRLAPNYPLHNYYKRITGMSSEGSDVKTGWIIPDRKTLISREEKQLEKNLQELATVLAPLYKQMAPVAYQNQVEYEEVAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPEDEQLHVLPLYRLADTDEFGSVEGMKAKIKSGAIQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGTAGLRRKRISASPKGAPGSHNTKSFSSASSTSHLVKDESTDFCPLQASSAETSTCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGECTGTVQPAEVAAHPHQSLPTADSPVHAEPLTSPSEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEPQHPEDDNLPQLDEFWSDSEEIYADPSFGGVAIAPIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFYQHKSLNKPNHGFDINKIKCKCKKVTKKKPADRECPDVSPEANLSHQIPSRVASTLTRDNVVTVSPYSLTHVAGPYNRWV

Target ORF information:

RefSeq Version	XM_006513867.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513867.4

ORF Insert Sequence:

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG 
AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC 
CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT 
ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC 
GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG 
GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG 
CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA 
AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC 
CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT 
ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC 
TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA 
ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT 
CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC 
CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG 
CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT 
GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA 
GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC 
TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC 
AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA 
AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT 
TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC 
GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tet1 ( NM_027384.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081660.1 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 2, mRNA.

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CloneID	OMu20453
Clone ID Related Accession (Same CDS sequence)	NM_027384.1
Accession Version	NM_027384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-22
Organism	Mus musculus(House mouse)
Product	methylcytosine dioxygenase TET1 isoform 2
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166359.3, CF897091.1, AK141438.1, AI605989.1 and AK010953.1. On or before Jun 25, 2009 this sequence version replaced XM_907080.3, XM_125673.7. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849381 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATGA AAAACAGCTT 
GAAAAGAATT TACAAGAATT GGCTACAGTA TTAGCTCCAC TTTACAAGCA GATGGCTCCA 
GTTGCTTATC AAAATCAGGT GGAATATGAA GAAGTTGCTG GAGACTGTCG ACTTGGAAAT 
GAAGAGGGGC GTCCTTTCTC TGGTGTCACC TGTTGCATGG ATTTTTGTGC CCATTCTCAC 
AAGGACATTC ACAACATGCA CAACGGAAGC ACCGTGGTGT GTACGTTGAT TCGAGCAGAT 
GGCCGTGACA CAAATTGTCC CGAGGATGAA CAACTCCACG TCCTGCCACT ATACCGGCTT 
GCAGACACTG ATGAATTTGG CTCCGTGGAA GGGATGAAGG CCAAAATCAA ATCTGGGGCC 
ATCCAAGTCA ATGGGCCAAC CAGGAAGAGG CGACTACGTT TTACTGAGCC TGTTCCTCGA 
TGTGGGAAGA GGGCCAAAAT GAAGCAGAAC CACAATAAAT CAGGTTCACA CAACACTAAG 
AGCTTTTCAT CAGCCTCATC TACTTCTCAC CTAGTGAAAG ACGAATCTAC AGACTTCTGT 
CCCCTGCAGG CTTCCTCCGC AGAAACATCT ACCTGTACGT ACAGTAAAAC AGCCTCAGGT 
GGGTTTGCAG AAACAAGTAG TATTCTCCAC TGCACAATGC CTTCTGGAGC ACACAGTGGT 
GCTAATGCAG CTGCTGGGGA ATGTACTGGA ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT 
CCTCACCAGT CTCTTCCCAC AGCCGATTCT CCCGTTCATG CTGAGCCTCT CACTAGTCCA 
TCTGAGCAGC TAACTTCTAA CCAGTCAAAC CAGCAGCTCC CTCTCCTCAG CAATTCTCAG 
AAACTGGCTT CCTGTCAGGT GGAAGATGAG CGGCACCCTG AAGCGGATGA GCCTCAGCAC 
CCCGAGGACG ATAACTTGCC TCAACTTGAT GAATTCTGGT CAGACAGTGA GGAGATCTAC 
GCCGATCCTT CCTTTGGTGG CGTGGCGATA GCACCCATTC ACGGCTCGGT GCTCATTGAG 
TGCGCTCGGA AGGAGCTTCA TGCTACCACC TCTTTGCGCT CCCCCAAACG AGGGGTCCCT 
TTTCGTGTGT CCCTTGTATT CTACCAGCAC AAAAGCCTAA ACAAGCCTAA TCATGGTTTT 
GATATCAACA AAATTAAGTG TAAATGCAAA AAAGTAACGA AAAAAAAGCC CGCAGACCGG 
GAGTGTCCTG ATGTATCCCC CGAAGCCAAT TTATCACACC AAATTCCTTC TCGAGTTGCA 
TCAACCTTAA CCCGAGACAA TGTTGTTACC GTGTCCCCAT ACTCTCTCAC TCATGTTGCG 
GGACCCTACA ATCGTTGGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081660.1
CDS	1..6024
Translation	MSRSRPAKPSKSVKTKLQKKKDIQMKTKTSKQAVRHGASAKAVNPGKPKQLIKRRDGKKETEDKTPTPAPSFLTRAGAARMNRDRNQVLFQNPDSLTCNGFTMALRRTSLSWRLSQRPVVTPKPKKVPPSKKQCTHNIQDEPGVKHSENDSVPSQHATVSPGTENGEQNRCLVEGESQEITQSCPVFEERIEDTQSCISASGNLEAEISWPLEGTHCEELLSHQTSDNECTSPQECAPLPQRSTSEVTSQKNTSNQLADLSSQVESIKLSDPSPNPTGSDHNGFPDSSFRIVPELDLKTCMPLDESVYPTALIRFILAGSQPDVFDTKPQEKTLITTPEQVGSHPNQVLDATSVLGQAFSTLPLQWGFSGANLVQVEALGKGSDSPEDLGAITMLNQQETVAMDMDRNATPDLPIFLPKPPNTVATYSSPLLGPEPHSSTSCGLEVQGATPILTLDSGHTPQLPPNPESSSVPLVIAANGTRAEKQFGTSLFPAVPQGFTVAAENEVQHAPLDLTQGSQAAPSKLEGEISRVSITGSADVKATAMSMPVTQASTSSPPCNSTPPMVERRKRKACGVCEPCQQKANCGECTYCKNRKNSHQICKKRKCEVLKKKPEATSQAQVTKENKRPQREKKPKVLKTDFNNKPVNGPKSESMDCSRRGHGEEEQRLDLITHPLENVRKNAGGMTGIEVEKWAPNKKSHLAEGQVKGSCDANLTGVENPQPSEDDKQQTNPSPTFAQTIRNGMKNVHCLPTDTHLPLNKLNHEEFSKALGNNSSKLLTDPSNCKDAMSVTTSGGECDHLKGPRNTLLFQKPGLNCRSGAEPTIFNNHPNTHSAGSRPHPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSESSSPSKPEKDEEAHQKTASLLNSCKAILHSVRKDLQDPNVQGKGLHHDTVVFNGQNRTFKSPDSFATNQALIKSQGYPSSPTAEKKGAAGGRAPFDGFENSHPLPIESHNLENCSQVLSCDQNLSSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINAEVRNAESTPESLVAKNTKQKHSQEKRMVHQKPPSSTQTKPSVPSAKPKKAQKKARATPHANKRKKKPPARSSQENDQKKQEQLAIEYSKMHDIWMSSKFQRFGQSSPRSFPVLLRNIPVFNQILKPVTQSKTPSQHNELFPPINQIKFTRNPELAKEKVKVEPSDSLPTCQFKTESGGQTFAEPADNSQGQPMVSVNQEAHPLPQSPPSNQCANIMAGAAQTQFHLGAQENLVHQIPPPTLPGTSPDTLLPDPASILRKGKVLHFDGITVVTEKREAQTSSNGPLGPTTDSAQSEFKESIMDLLSKPAKNLIAGLKEQEAAPCDCDGGTQKEKGPYYTHLGAGPSVAAVRELMETRFGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKLICLVRERVDHHCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGIDPKTCGASFSFGCSWSMYFNGCKFGRSENPRKFRLAPNYPLHEKQLEKNLQELATVLAPLYKQMAPVAYQNQVEYEEVAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPEDEQLHVLPLYRLADTDEFGSVEGMKAKIKSGAIQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGSHNTKSFSSASSTSHLVKDESTDFCPLQASSAETSTCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGECTGTVQPAEVAAHPHQSLPTADSPVHAEPLTSPSEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEPQHPEDDNLPQLDEFWSDSEEIYADPSFGGVAIAPIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFYQHKSLNKPNHGFDINKIKCKCKKVTKKKPADRECPDVSPEANLSHQIPSRVASTLTRDNVVTVSPYSLTHVAGPYNRWV

Target ORF information:

RefSeq Version	NM_027384.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027384.1

ORF Insert Sequence:

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATGA AAAACAGCTT 
GAAAAGAATT TACAAGAATT GGCTACAGTA TTAGCTCCAC TTTACAAGCA GATGGCTCCA 
GTTGCTTATC AAAATCAGGT GGAATATGAA GAAGTTGCTG GAGACTGTCG ACTTGGAAAT 
GAAGAGGGGC GTCCTTTCTC TGGTGTCACC TGTTGCATGG ATTTTTGTGC CCATTCTCAC 
AAGGACATTC ACAACATGCA CAACGGAAGC ACCGTGGTGT GTACGTTGAT TCGAGCAGAT 
GGCCGTGACA CAAATTGTCC CGAGGATGAA CAACTCCACG TCCTGCCACT ATACCGGCTT 
GCAGACACTG ATGAATTTGG CTCCGTGGAA GGGATGAAGG CCAAAATCAA ATCTGGGGCC 
ATCCAAGTCA ATGGGCCAAC CAGGAAGAGG CGACTACGTT TTACTGAGCC TGTTCCTCGA 
TGTGGGAAGA GGGCCAAAAT GAAGCAGAAC CACAATAAAT CAGGTTCACA CAACACTAAG 
AGCTTTTCAT CAGCCTCATC TACTTCTCAC CTAGTGAAAG ACGAATCTAC AGACTTCTGT 
CCCCTGCAGG CTTCCTCCGC AGAAACATCT ACCTGTACGT ACAGTAAAAC AGCCTCAGGT 
GGGTTTGCAG AAACAAGTAG TATTCTCCAC TGCACAATGC CTTCTGGAGC ACACAGTGGT 
GCTAATGCAG CTGCTGGGGA ATGTACTGGA ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT 
CCTCACCAGT CTCTTCCCAC AGCCGATTCT CCCGTTCATG CTGAGCCTCT CACTAGTCCA 
TCTGAGCAGC TAACTTCTAA CCAGTCAAAC CAGCAGCTCC CTCTCCTCAG CAATTCTCAG 
AAACTGGCTT CCTGTCAGGT GGAAGATGAG CGGCACCCTG AAGCGGATGA GCCTCAGCAC 
CCCGAGGACG ATAACTTGCC TCAACTTGAT GAATTCTGGT CAGACAGTGA GGAGATCTAC 
GCCGATCCTT CCTTTGGTGG CGTGGCGATA GCACCCATTC ACGGCTCGGT GCTCATTGAG 
TGCGCTCGGA AGGAGCTTCA TGCTACCACC TCTTTGCGCT CCCCCAAACG AGGGGTCCCT 
TTTCGTGTGT CCCTTGTATT CTACCAGCAC AAAAGCCTAA ACAAGCCTAA TCATGGTTTT 
GATATCAACA AAATTAAGTG TAAATGCAAA AAAGTAACGA AAAAAAAGCC CGCAGACCGG 
GAGTGTCCTG ATGTATCCCC CGAAGCCAAT TTATCACACC AAATTCCTTC TCGAGTTGCA 
TCAACCTTAA CCCGAGACAA TGTTGTTACC GTGTCCCCAT ACTCTCTCAC TCATGTTGCG 
GGACCCTACA ATCGTTGGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tet1 ( NM_001253857.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001240786.1 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 1, mRNA.

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CloneID	OMu20533
Clone ID Related Accession (Same CDS sequence)	XM_011243509.2 , NM_001253857.2 , NM_001253857.1
Accession Version	NM_001253857.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	methylcytosine dioxygenase TET1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166359.3. On or before Jun 22, 2017 this sequence version replaced XM_011243509.2, NM_001253857.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: GU079948.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG 
AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC 
CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT 
ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC 
GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG 
GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG 
CAGAACCACA ATAAATCAGG TTCACACAAC ACTAAGAGCT TTTCATCAGC CTCATCTACT 
TCTCACCTAG TGAAAGACGA ATCTACAGAC TTCTGTCCCC TGCAGGCTTC CTCCGCAGAA 
ACATCTACCT GTACGTACAG TAAAACAGCC TCAGGTGGGT TTGCAGAAAC AAGTAGTATT 
CTCCACTGCA CAATGCCTTC TGGAGCACAC AGTGGTGCTA ATGCAGCTGC TGGGGAATGT 
ACTGGAACGG TGCAGCCTGC CGAGGTGGCT GCTCATCCTC ACCAGTCTCT TCCCACAGCC 
GATTCTCCCG TTCATGCTGA GCCTCTCACT AGTCCATCTG AGCAGCTAAC TTCTAACCAG 
TCAAACCAGC AGCTCCCTCT CCTCAGCAAT TCTCAGAAAC TGGCTTCCTG TCAGGTGGAA 
GATGAGCGGC ACCCTGAAGC GGATGAGCCT CAGCACCCCG AGGACGATAA CTTGCCTCAA 
CTTGATGAAT TCTGGTCAGA CAGTGAGGAG ATCTACGCCG ATCCTTCCTT TGGTGGCGTG 
GCGATAGCAC CCATTCACGG CTCGGTGCTC ATTGAGTGCG CTCGGAAGGA GCTTCATGCT 
ACCACCTCTT TGCGCTCCCC CAAACGAGGG GTCCCTTTTC GTGTGTCCCT TGTATTCTAC 
CAGCACAAAA GCCTAAACAA GCCTAATCAT GGTTTTGATA TCAACAAAAT TAAGTGTAAA 
TGCAAAAAAG TAACGAAAAA AAAGCCCGCA GACCGGGAGT GTCCTGATGT ATCCCCCGAA 
GCCAATTTAT CACACCAAAT TCCTTCTCGA GTTGCATCAA CCTTAACCCG AGACAATGTT 
GTTACCGTGT CCCCATACTC TCTCACTCAT GTTGCGGGAC CCTACAATCG TTGGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240786.1
CDS	273..6392
Translation	MSRSRPAKPSKSVKTKLQKKKDIQMKTKTSKQAVRHGASAKAVNPGKPKQLIKRRDGKKETEDKTPTPAPSFLTRAGAARMNRDRNQVLFQNPDSLTCNGFTMALRRTSLSWRLSQRPVVTPKPKKVPPSKKQCTHNIQDEPGVKHSENDSVPSQHATVSPGTENGEQNRCLVEGESQEITQSCPVFEERIEDTQSCISASGNLEAEISWPLEGTHCEELLSHQTSDNECTSPQECAPLPQRSTSEVTSQKNTSNQLADLSSQVESIKLSDPSPNPTGSDHNGFPDSSFRIVPELDLKTCMPLDESVYPTALIRFILAGSQPDVFDTKPQEKTLITTPEQVGSHPNQVLDATSVLGQAFSTLPLQWGFSGANLVQVEALGKGSDSPEDLGAITMLNQQETVAMDMDRNATPDLPIFLPKPPNTVATYSSPLLGPEPHSSTSCGLEVQGATPILTLDSGHTPQLPPNPESSSVPLVIAANGTRAEKQFGTSLFPAVPQGFTVAAENEVQHAPLDLTQGSQAAPSKLEGEISRVSITGSADVKATAMSMPVTQASTSSPPCNSTPPMVERRKRKACGVCEPCQQKANCGECTYCKNRKNSHQICKKRKCEVLKKKPEATSQAQVTKENKRPQREKKPKVLKTDFNNKPVNGPKSESMDCSRRGHGEEEQRLDLITHPLENVRKNAGGMTGIEVEKWAPNKKSHLAEGQVKGSCDANLTGVENPQPSEDDKQQTNPSPTFAQTIRNGMKNVHCLPTDTHLPLNKLNHEEFSKALGNNSSKLLTDPSNCKDAMSVTTSGGECDHLKGPRNTLLFQKPGLNCRSGAEPTIFNNHPNTHSAGSRPHPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSESSSPSKPEKDEEAHQKTASLLNSCKAILHSVRKDLQDPNVQGKGLHHDTVVFNGQNRTFKSPDSFATNQALIKSQGYPSSPTAEKKGAAGGRAPFDGFENSHPLPIESHNLENCSQVLSCDQNLSSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINAEVRNAESTPESLVAKNTKQKHSQEKRMVHQKPPSSTQTKPSVPSAKPKKAQKKARATPHANKRKKKPPARSSQENDQKKQEQLAIEYSKMHDIWMSSKFQRFGQSSPRSFPVLLRNIPVFNQILKPVTQSKTPSQHNELFPPINQIKFTRNPELAKEKVKVEPSDSLPTCQFKTESGGQTFAEPADNSQGQPMVSVNQEAHPLPQSPPSNQCANIMAGAAQTQFHLGAQENLVHQIPPPTLPGTSPDTLLPDPASILRKGKVLHFDGITVVTEKREAQTSSNGPLGPTTDSAQSEFKESIMDLLSKPAKNLIAGLKEQEAAPCDCDGGTQKEKGPYYTHLGAGPSVAAVRELMETRFGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKLICLVRERVDHHCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGIDPKTCGASFSFGCSWSMYFNGCKFGRSENPRKFRLAPNYPLHNYYKRITGMSSEGSDVKTGWIIPDRKTLISREEKQLEKNLQELATVLAPLYKQMAPVAYQNQVEYEEVAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPEDEQLHVLPLYRLADTDEFGSVEGMKAKIKSGAIQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGSHNTKSFSSASSTSHLVKDESTDFCPLQASSAETSTCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGECTGTVQPAEVAAHPHQSLPTADSPVHAEPLTSPSEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEPQHPEDDNLPQLDEFWSDSEEIYADPSFGGVAIAPIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFYQHKSLNKPNHGFDINKIKCKCKKVTKKKPADRECPDVSPEANLSHQIPSRVASTLTRDNVVTVSPYSLTHVAGPYNRWV

Target ORF information:

RefSeq Version	NM_001253857.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253857.2

ORF Insert Sequence:

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG 
AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC 
CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT 
ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC 
GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG 
GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG 
CAGAACCACA ATAAATCAGG TTCACACAAC ACTAAGAGCT TTTCATCAGC CTCATCTACT 
TCTCACCTAG TGAAAGACGA ATCTACAGAC TTCTGTCCCC TGCAGGCTTC CTCCGCAGAA 
ACATCTACCT GTACGTACAG TAAAACAGCC TCAGGTGGGT TTGCAGAAAC AAGTAGTATT 
CTCCACTGCA CAATGCCTTC TGGAGCACAC AGTGGTGCTA ATGCAGCTGC TGGGGAATGT 
ACTGGAACGG TGCAGCCTGC CGAGGTGGCT GCTCATCCTC ACCAGTCTCT TCCCACAGCC 
GATTCTCCCG TTCATGCTGA GCCTCTCACT AGTCCATCTG AGCAGCTAAC TTCTAACCAG 
TCAAACCAGC AGCTCCCTCT CCTCAGCAAT TCTCAGAAAC TGGCTTCCTG TCAGGTGGAA 
GATGAGCGGC ACCCTGAAGC GGATGAGCCT CAGCACCCCG AGGACGATAA CTTGCCTCAA 
CTTGATGAAT TCTGGTCAGA CAGTGAGGAG ATCTACGCCG ATCCTTCCTT TGGTGGCGTG 
GCGATAGCAC CCATTCACGG CTCGGTGCTC ATTGAGTGCG CTCGGAAGGA GCTTCATGCT 
ACCACCTCTT TGCGCTCCCC CAAACGAGGG GTCCCTTTTC GTGTGTCCCT TGTATTCTAC 
CAGCACAAAA GCCTAAACAA GCCTAATCAT GGTTTTGATA TCAACAAAAT TAAGTGTAAA 
TGCAAAAAAG TAACGAAAAA AAAGCCCGCA GACCGGGAGT GTCCTGATGT ATCCCCCGAA 
GCCAATTTAT CACACCAAAT TCCTTCTCGA GTTGCATCAA CCTTAACCCG AGACAATGTT 
GTTACCGTGT CCCCATACTC TCTCACTCAT GTTGCGGGAC CCTACAATCG TTGGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tet1 ( NM_001253857.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001240786.1 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 1, mRNA.

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CloneID	OMu20533
Clone ID Related Accession (Same CDS sequence)	XM_011243509.2 , NM_001253857.2 , NM_001253857.1
Accession Version	NM_001253857.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-22
Organism	Mus musculus(House mouse)
Product	methylcytosine dioxygenase TET1 isoform 1
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166359.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GU079948.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02146468 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG 
AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC 
CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT 
ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC 
GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG 
GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG 
CAGAACCACA ATAAATCAGG TTCACACAAC ACTAAGAGCT TTTCATCAGC CTCATCTACT 
TCTCACCTAG TGAAAGACGA ATCTACAGAC TTCTGTCCCC TGCAGGCTTC CTCCGCAGAA 
ACATCTACCT GTACGTACAG TAAAACAGCC TCAGGTGGGT TTGCAGAAAC AAGTAGTATT 
CTCCACTGCA CAATGCCTTC TGGAGCACAC AGTGGTGCTA ATGCAGCTGC TGGGGAATGT 
ACTGGAACGG TGCAGCCTGC CGAGGTGGCT GCTCATCCTC ACCAGTCTCT TCCCACAGCC 
GATTCTCCCG TTCATGCTGA GCCTCTCACT AGTCCATCTG AGCAGCTAAC TTCTAACCAG 
TCAAACCAGC AGCTCCCTCT CCTCAGCAAT TCTCAGAAAC TGGCTTCCTG TCAGGTGGAA 
GATGAGCGGC ACCCTGAAGC GGATGAGCCT CAGCACCCCG AGGACGATAA CTTGCCTCAA 
CTTGATGAAT TCTGGTCAGA CAGTGAGGAG ATCTACGCCG ATCCTTCCTT TGGTGGCGTG 
GCGATAGCAC CCATTCACGG CTCGGTGCTC ATTGAGTGCG CTCGGAAGGA GCTTCATGCT 
ACCACCTCTT TGCGCTCCCC CAAACGAGGG GTCCCTTTTC GTGTGTCCCT TGTATTCTAC 
CAGCACAAAA GCCTAAACAA GCCTAATCAT GGTTTTGATA TCAACAAAAT TAAGTGTAAA 
TGCAAAAAAG TAACGAAAAA AAAGCCCGCA GACCGGGAGT GTCCTGATGT ATCCCCCGAA 
GCCAATTTAT CACACCAAAT TCCTTCTCGA GTTGCATCAA CCTTAACCCG AGACAATGTT 
GTTACCGTGT CCCCATACTC TCTCACTCAT GTTGCGGGAC CCTACAATCG TTGGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240786.1
CDS	1..6120
Translation	MSRSRPAKPSKSVKTKLQKKKDIQMKTKTSKQAVRHGASAKAVNPGKPKQLIKRRDGKKETEDKTPTPAPSFLTRAGAARMNRDRNQVLFQNPDSLTCNGFTMALRRTSLSWRLSQRPVVTPKPKKVPPSKKQCTHNIQDEPGVKHSENDSVPSQHATVSPGTENGEQNRCLVEGESQEITQSCPVFEERIEDTQSCISASGNLEAEISWPLEGTHCEELLSHQTSDNECTSPQECAPLPQRSTSEVTSQKNTSNQLADLSSQVESIKLSDPSPNPTGSDHNGFPDSSFRIVPELDLKTCMPLDESVYPTALIRFILAGSQPDVFDTKPQEKTLITTPEQVGSHPNQVLDATSVLGQAFSTLPLQWGFSGANLVQVEALGKGSDSPEDLGAITMLNQQETVAMDMDRNATPDLPIFLPKPPNTVATYSSPLLGPEPHSSTSCGLEVQGATPILTLDSGHTPQLPPNPESSSVPLVIAANGTRAEKQFGTSLFPAVPQGFTVAAENEVQHAPLDLTQGSQAAPSKLEGEISRVSITGSADVKATAMSMPVTQASTSSPPCNSTPPMVERRKRKACGVCEPCQQKANCGECTYCKNRKNSHQICKKRKCEVLKKKPEATSQAQVTKENKRPQREKKPKVLKTDFNNKPVNGPKSESMDCSRRGHGEEEQRLDLITHPLENVRKNAGGMTGIEVEKWAPNKKSHLAEGQVKGSCDANLTGVENPQPSEDDKQQTNPSPTFAQTIRNGMKNVHCLPTDTHLPLNKLNHEEFSKALGNNSSKLLTDPSNCKDAMSVTTSGGECDHLKGPRNTLLFQKPGLNCRSGAEPTIFNNHPNTHSAGSRPHPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSESSSPSKPEKDEEAHQKTASLLNSCKAILHSVRKDLQDPNVQGKGLHHDTVVFNGQNRTFKSPDSFATNQALIKSQGYPSSPTAEKKGAAGGRAPFDGFENSHPLPIESHNLENCSQVLSCDQNLSSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINAEVRNAESTPESLVAKNTKQKHSQEKRMVHQKPPSSTQTKPSVPSAKPKKAQKKARATPHANKRKKKPPARSSQENDQKKQEQLAIEYSKMHDIWMSSKFQRFGQSSPRSFPVLLRNIPVFNQILKPVTQSKTPSQHNELFPPINQIKFTRNPELAKEKVKVEPSDSLPTCQFKTESGGQTFAEPADNSQGQPMVSVNQEAHPLPQSPPSNQCANIMAGAAQTQFHLGAQENLVHQIPPPTLPGTSPDTLLPDPASILRKGKVLHFDGITVVTEKREAQTSSNGPLGPTTDSAQSEFKESIMDLLSKPAKNLIAGLKEQEAAPCDCDGGTQKEKGPYYTHLGAGPSVAAVRELMETRFGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKLICLVRERVDHHCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGIDPKTCGASFSFGCSWSMYFNGCKFGRSENPRKFRLAPNYPLHNYYKRITGMSSEGSDVKTGWIIPDRKTLISREEKQLEKNLQELATVLAPLYKQMAPVAYQNQVEYEEVAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPEDEQLHVLPLYRLADTDEFGSVEGMKAKIKSGAIQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGSHNTKSFSSASSTSHLVKDESTDFCPLQASSAETSTCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGECTGTVQPAEVAAHPHQSLPTADSPVHAEPLTSPSEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEPQHPEDDNLPQLDEFWSDSEEIYADPSFGGVAIAPIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFYQHKSLNKPNHGFDINKIKCKCKKVTKKKPADRECPDVSPEANLSHQIPSRVASTLTRDNVVTVSPYSLTHVAGPYNRWV

Target ORF information:

RefSeq Version	NM_001253857.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253857.1

ORF Insert Sequence:

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG 
AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC 
CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT 
ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC 
GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG 
GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG 
CAGAACCACA ATAAATCAGG TTCACACAAC ACTAAGAGCT TTTCATCAGC CTCATCTACT 
TCTCACCTAG TGAAAGACGA ATCTACAGAC TTCTGTCCCC TGCAGGCTTC CTCCGCAGAA 
ACATCTACCT GTACGTACAG TAAAACAGCC TCAGGTGGGT TTGCAGAAAC AAGTAGTATT 
CTCCACTGCA CAATGCCTTC TGGAGCACAC AGTGGTGCTA ATGCAGCTGC TGGGGAATGT 
ACTGGAACGG TGCAGCCTGC CGAGGTGGCT GCTCATCCTC ACCAGTCTCT TCCCACAGCC 
GATTCTCCCG TTCATGCTGA GCCTCTCACT AGTCCATCTG AGCAGCTAAC TTCTAACCAG 
TCAAACCAGC AGCTCCCTCT CCTCAGCAAT TCTCAGAAAC TGGCTTCCTG TCAGGTGGAA 
GATGAGCGGC ACCCTGAAGC GGATGAGCCT CAGCACCCCG AGGACGATAA CTTGCCTCAA 
CTTGATGAAT TCTGGTCAGA CAGTGAGGAG ATCTACGCCG ATCCTTCCTT TGGTGGCGTG 
GCGATAGCAC CCATTCACGG CTCGGTGCTC ATTGAGTGCG CTCGGAAGGA GCTTCATGCT 
ACCACCTCTT TGCGCTCCCC CAAACGAGGG GTCCCTTTTC GTGTGTCCCT TGTATTCTAC 
CAGCACAAAA GCCTAAACAA GCCTAATCAT GGTTTTGATA TCAACAAAAT TAAGTGTAAA 
TGCAAAAAAG TAACGAAAAA AAAGCCCGCA GACCGGGAGT GTCCTGATGT ATCCCCCGAA 
GCCAATTTAT CACACCAAAT TCCTTCTCGA GTTGCATCAA CCTTAACCCG AGACAATGTT 
GTTACCGTGT CCCCATACTC TCTCACTCAT GTTGCGGGAC CCTACAATCG TTGGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tet1 ( XM_011243509.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241811.1 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X4, mRNA.

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CloneID	OMu20533
Clone ID Related Accession (Same CDS sequence)	XM_011243509.2 , NM_001253857.2 , NM_001253857.1
Accession Version	XM_011243509.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	methylcytosine dioxygenase TET1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243509.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG 
AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC 
CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT 
ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC 
GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG 
GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG 
CAGAACCACA ATAAATCAGG TTCACACAAC ACTAAGAGCT TTTCATCAGC CTCATCTACT 
TCTCACCTAG TGAAAGACGA ATCTACAGAC TTCTGTCCCC TGCAGGCTTC CTCCGCAGAA 
ACATCTACCT GTACGTACAG TAAAACAGCC TCAGGTGGGT TTGCAGAAAC AAGTAGTATT 
CTCCACTGCA CAATGCCTTC TGGAGCACAC AGTGGTGCTA ATGCAGCTGC TGGGGAATGT 
ACTGGAACGG TGCAGCCTGC CGAGGTGGCT GCTCATCCTC ACCAGTCTCT TCCCACAGCC 
GATTCTCCCG TTCATGCTGA GCCTCTCACT AGTCCATCTG AGCAGCTAAC TTCTAACCAG 
TCAAACCAGC AGCTCCCTCT CCTCAGCAAT TCTCAGAAAC TGGCTTCCTG TCAGGTGGAA 
GATGAGCGGC ACCCTGAAGC GGATGAGCCT CAGCACCCCG AGGACGATAA CTTGCCTCAA 
CTTGATGAAT TCTGGTCAGA CAGTGAGGAG ATCTACGCCG ATCCTTCCTT TGGTGGCGTG 
GCGATAGCAC CCATTCACGG CTCGGTGCTC ATTGAGTGCG CTCGGAAGGA GCTTCATGCT 
ACCACCTCTT TGCGCTCCCC CAAACGAGGG GTCCCTTTTC GTGTGTCCCT TGTATTCTAC 
CAGCACAAAA GCCTAAACAA GCCTAATCAT GGTTTTGATA TCAACAAAAT TAAGTGTAAA 
TGCAAAAAAG TAACGAAAAA AAAGCCCGCA GACCGGGAGT GTCCTGATGT ATCCCCCGAA 
GCCAATTTAT CACACCAAAT TCCTTCTCGA GTTGCATCAA CCTTAACCCG AGACAATGTT 
GTTACCGTGT CCCCATACTC TCTCACTCAT GTTGCGGGAC CCTACAATCG TTGGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241811.1
CDS	518..6637
Translation	MSRSRPAKPSKSVKTKLQKKKDIQMKTKTSKQAVRHGASAKAVNPGKPKQLIKRRDGKKETEDKTPTPAPSFLTRAGAARMNRDRNQVLFQNPDSLTCNGFTMALRRTSLSWRLSQRPVVTPKPKKVPPSKKQCTHNIQDEPGVKHSENDSVPSQHATVSPGTENGEQNRCLVEGESQEITQSCPVFEERIEDTQSCISASGNLEAEISWPLEGTHCEELLSHQTSDNECTSPQECAPLPQRSTSEVTSQKNTSNQLADLSSQVESIKLSDPSPNPTGSDHNGFPDSSFRIVPELDLKTCMPLDESVYPTALIRFILAGSQPDVFDTKPQEKTLITTPEQVGSHPNQVLDATSVLGQAFSTLPLQWGFSGANLVQVEALGKGSDSPEDLGAITMLNQQETVAMDMDRNATPDLPIFLPKPPNTVATYSSPLLGPEPHSSTSCGLEVQGATPILTLDSGHTPQLPPNPESSSVPLVIAANGTRAEKQFGTSLFPAVPQGFTVAAENEVQHAPLDLTQGSQAAPSKLEGEISRVSITGSADVKATAMSMPVTQASTSSPPCNSTPPMVERRKRKACGVCEPCQQKANCGECTYCKNRKNSHQICKKRKCEVLKKKPEATSQAQVTKENKRPQREKKPKVLKTDFNNKPVNGPKSESMDCSRRGHGEEEQRLDLITHPLENVRKNAGGMTGIEVEKWAPNKKSHLAEGQVKGSCDANLTGVENPQPSEDDKQQTNPSPTFAQTIRNGMKNVHCLPTDTHLPLNKLNHEEFSKALGNNSSKLLTDPSNCKDAMSVTTSGGECDHLKGPRNTLLFQKPGLNCRSGAEPTIFNNHPNTHSAGSRPHPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSESSSPSKPEKDEEAHQKTASLLNSCKAILHSVRKDLQDPNVQGKGLHHDTVVFNGQNRTFKSPDSFATNQALIKSQGYPSSPTAEKKGAAGGRAPFDGFENSHPLPIESHNLENCSQVLSCDQNLSSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINAEVRNAESTPESLVAKNTKQKHSQEKRMVHQKPPSSTQTKPSVPSAKPKKAQKKARATPHANKRKKKPPARSSQENDQKKQEQLAIEYSKMHDIWMSSKFQRFGQSSPRSFPVLLRNIPVFNQILKPVTQSKTPSQHNELFPPINQIKFTRNPELAKEKVKVEPSDSLPTCQFKTESGGQTFAEPADNSQGQPMVSVNQEAHPLPQSPPSNQCANIMAGAAQTQFHLGAQENLVHQIPPPTLPGTSPDTLLPDPASILRKGKVLHFDGITVVTEKREAQTSSNGPLGPTTDSAQSEFKESIMDLLSKPAKNLIAGLKEQEAAPCDCDGGTQKEKGPYYTHLGAGPSVAAVRELMETRFGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKLICLVRERVDHHCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGIDPKTCGASFSFGCSWSMYFNGCKFGRSENPRKFRLAPNYPLHNYYKRITGMSSEGSDVKTGWIIPDRKTLISREEKQLEKNLQELATVLAPLYKQMAPVAYQNQVEYEEVAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPEDEQLHVLPLYRLADTDEFGSVEGMKAKIKSGAIQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGSHNTKSFSSASSTSHLVKDESTDFCPLQASSAETSTCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGECTGTVQPAEVAAHPHQSLPTADSPVHAEPLTSPSEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEPQHPEDDNLPQLDEFWSDSEEIYADPSFGGVAIAPIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFYQHKSLNKPNHGFDINKIKCKCKKVTKKKPADRECPDVSPEANLSHQIPSRVASTLTRDNVVTVSPYSLTHVAGPYNRWV

Target ORF information:

RefSeq Version	XM_011243509.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243509.2

ORF Insert Sequence:

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG 
AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC 
CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT 
ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC 
GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG 
GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG 
CAGAACCACA ATAAATCAGG TTCACACAAC ACTAAGAGCT TTTCATCAGC CTCATCTACT 
TCTCACCTAG TGAAAGACGA ATCTACAGAC TTCTGTCCCC TGCAGGCTTC CTCCGCAGAA 
ACATCTACCT GTACGTACAG TAAAACAGCC TCAGGTGGGT TTGCAGAAAC AAGTAGTATT 
CTCCACTGCA CAATGCCTTC TGGAGCACAC AGTGGTGCTA ATGCAGCTGC TGGGGAATGT 
ACTGGAACGG TGCAGCCTGC CGAGGTGGCT GCTCATCCTC ACCAGTCTCT TCCCACAGCC 
GATTCTCCCG TTCATGCTGA GCCTCTCACT AGTCCATCTG AGCAGCTAAC TTCTAACCAG 
TCAAACCAGC AGCTCCCTCT CCTCAGCAAT TCTCAGAAAC TGGCTTCCTG TCAGGTGGAA 
GATGAGCGGC ACCCTGAAGC GGATGAGCCT CAGCACCCCG AGGACGATAA CTTGCCTCAA 
CTTGATGAAT TCTGGTCAGA CAGTGAGGAG ATCTACGCCG ATCCTTCCTT TGGTGGCGTG 
GCGATAGCAC CCATTCACGG CTCGGTGCTC ATTGAGTGCG CTCGGAAGGA GCTTCATGCT 
ACCACCTCTT TGCGCTCCCC CAAACGAGGG GTCCCTTTTC GTGTGTCCCT TGTATTCTAC 
CAGCACAAAA GCCTAAACAA GCCTAATCAT GGTTTTGATA TCAACAAAAT TAAGTGTAAA 
TGCAAAAAAG TAACGAAAAA AAAGCCCGCA GACCGGGAGT GTCCTGATGT ATCCCCCGAA 
GCCAATTTAT CACACCAAAT TCCTTCTCGA GTTGCATCAA CCTTAACCCG AGACAATGTT 
GTTACCGTGT CCCCATACTC TCTCACTCAT GTTGCGGGAC CCTACAATCG TTGGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tet1 ( XM_006513867.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513930.1 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X1, mRNA.

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CloneID	OMu44647
Clone ID Related Accession (Same CDS sequence)	XM_006513867.3 , XM_011243508.2 , XM_006513867.4 , XM_006513869.3
Accession Version	XM_006513867.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methylcytosine dioxygenase TET1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513867.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG 
AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC 
CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT 
ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC 
GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG 
GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG 
CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA 
AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC 
CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT 
ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC 
TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA 
ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT 
CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC 
CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG 
CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT 
GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA 
GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC 
TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC 
AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA 
AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT 
TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC 
GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513930.1
CDS	519..6692
Translation	MSRSRPAKPSKSVKTKLQKKKDIQMKTKTSKQAVRHGASAKAVNPGKPKQLIKRRDGKKETEDKTPTPAPSFLTRAGAARMNRDRNQVLFQNPDSLTCNGFTMALRRTSLSWRLSQRPVVTPKPKKVPPSKKQCTHNIQDEPGVKHSENDSVPSQHATVSPGTENGEQNRCLVEGESQEITQSCPVFEERIEDTQSCISASGNLEAEISWPLEGTHCEELLSHQTSDNECTSPQECAPLPQRSTSEVTSQKNTSNQLADLSSQVESIKLSDPSPNPTGSDHNGFPDSSFRIVPELDLKTCMPLDESVYPTALIRFILAGSQPDVFDTKPQEKTLITTPEQVGSHPNQVLDATSVLGQAFSTLPLQWGFSGANLVQVEALGKGSDSPEDLGAITMLNQQETVAMDMDRNATPDLPIFLPKPPNTVATYSSPLLGPEPHSSTSCGLEVQGATPILTLDSGHTPQLPPNPESSSVPLVIAANGTRAEKQFGTSLFPAVPQGFTVAAENEVQHAPLDLTQGSQAAPSKLEGEISRVSITGSADVKATAMSMPVTQASTSSPPCNSTPPMVERRKRKACGVCEPCQQKANCGECTYCKNRKNSHQICKKRKCEVLKKKPEATSQAQVTKENKRPQREKKPKVLKTDFNNKPVNGPKSESMDCSRRGHGEEEQRLDLITHPLENVRKNAGGMTGIEVEKWAPNKKSHLAEGQVKGSCDANLTGVENPQPSEDDKQQTNPSPTFAQTIRNGMKNVHCLPTDTHLPLNKLNHEEFSKALGNNSSKLLTDPSNCKDAMSVTTSGGECDHLKGPRNTLLFQKPGLNCRSGAEPTIFNNHPNTHSAGSRPHPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSESSSPSKPEKDEEAHQKTASLLNSCKAILHSVRKDLQDPNVQGKGLHHDTVVFNGQNRTFKSPDSFATNQALIKSQGYPSSPTAEKKGAAGGRAPFDGFENSHPLPIESHNLENCSQVLSCDQNLSSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINAEVRNAESTPESLVAKNTKQKHSQEKRMVHQKPPSSTQTKPSVPSAKPKKAQKKARATPHANKRKKKPPARSSQENDQKKQEQLAIEYSKMHDIWMSSKFQRFGQSSPRSFPVLLRNIPVFNQILKPVTQSKTPSQHNELFPPINQIKFTRNPELAKEKVKVEPSDSLPTCQFKTESGGQTFAEPADNSQGQPMVSVNQEAHPLPQSPPSNQCANIMAGAAQTQFHLGAQENLVHQIPPPTLPGTSPDTLLPDPASILRKGKVLHFDGITVVTEKREAQTSSNGPLGPTTDSAQSEFKESIMDLLSKPAKNLIAGLKEQEAAPCDCDGGTQKEKGPYYTHLGAGPSVAAVRELMETRFGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKLICLVRERVDHHCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGIDPKTCGASFSFGCSWSMYFNGCKFGRSENPRKFRLAPNYPLHNYYKRITGMSSEGSDVKTGWIIPDRKTLISREEKQLEKNLQELATVLAPLYKQMAPVAYQNQVEYEEVAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPEDEQLHVLPLYRLADTDEFGSVEGMKAKIKSGAIQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGTAGLRRKRISASPKGAPGSHNTKSFSSASSTSHLVKDESTDFCPLQASSAETSTCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGECTGTVQPAEVAAHPHQSLPTADSPVHAEPLTSPSEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEPQHPEDDNLPQLDEFWSDSEEIYADPSFGGVAIAPIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFYQHKSLNKPNHGFDINKIKCKCKKVTKKKPADRECPDVSPEANLSHQIPSRVASTLTRDNVVTVSPYSLTHVAGPYNRWV

Target ORF information:

RefSeq Version	XM_006513867.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513867.3

ORF Insert Sequence:

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG 
AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC 
CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT 
ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC 
GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG 
GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG 
CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA 
AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC 
CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT 
ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC 
TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA 
ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT 
CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC 
CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG 
CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT 
GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA 
GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC 
TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC 
AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA 
AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT 
TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC 
GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tet1 ( XM_011243508.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241810.1 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X3, mRNA.

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CloneID	OMu44647
Clone ID Related Accession (Same CDS sequence)	XM_006513867.3 , XM_011243508.2 , XM_006513867.4 , XM_006513869.3
Accession Version	XM_011243508.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methylcytosine dioxygenase TET1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243508.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG 
AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC 
CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT 
ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC 
GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG 
GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG 
CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA 
AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC 
CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT 
ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC 
TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA 
ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT 
CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC 
CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG 
CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT 
GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA 
GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC 
TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC 
AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA 
AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT 
TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC 
GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241810.1
CDS	335..6508
Translation	MSRSRPAKPSKSVKTKLQKKKDIQMKTKTSKQAVRHGASAKAVNPGKPKQLIKRRDGKKETEDKTPTPAPSFLTRAGAARMNRDRNQVLFQNPDSLTCNGFTMALRRTSLSWRLSQRPVVTPKPKKVPPSKKQCTHNIQDEPGVKHSENDSVPSQHATVSPGTENGEQNRCLVEGESQEITQSCPVFEERIEDTQSCISASGNLEAEISWPLEGTHCEELLSHQTSDNECTSPQECAPLPQRSTSEVTSQKNTSNQLADLSSQVESIKLSDPSPNPTGSDHNGFPDSSFRIVPELDLKTCMPLDESVYPTALIRFILAGSQPDVFDTKPQEKTLITTPEQVGSHPNQVLDATSVLGQAFSTLPLQWGFSGANLVQVEALGKGSDSPEDLGAITMLNQQETVAMDMDRNATPDLPIFLPKPPNTVATYSSPLLGPEPHSSTSCGLEVQGATPILTLDSGHTPQLPPNPESSSVPLVIAANGTRAEKQFGTSLFPAVPQGFTVAAENEVQHAPLDLTQGSQAAPSKLEGEISRVSITGSADVKATAMSMPVTQASTSSPPCNSTPPMVERRKRKACGVCEPCQQKANCGECTYCKNRKNSHQICKKRKCEVLKKKPEATSQAQVTKENKRPQREKKPKVLKTDFNNKPVNGPKSESMDCSRRGHGEEEQRLDLITHPLENVRKNAGGMTGIEVEKWAPNKKSHLAEGQVKGSCDANLTGVENPQPSEDDKQQTNPSPTFAQTIRNGMKNVHCLPTDTHLPLNKLNHEEFSKALGNNSSKLLTDPSNCKDAMSVTTSGGECDHLKGPRNTLLFQKPGLNCRSGAEPTIFNNHPNTHSAGSRPHPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSESSSPSKPEKDEEAHQKTASLLNSCKAILHSVRKDLQDPNVQGKGLHHDTVVFNGQNRTFKSPDSFATNQALIKSQGYPSSPTAEKKGAAGGRAPFDGFENSHPLPIESHNLENCSQVLSCDQNLSSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINAEVRNAESTPESLVAKNTKQKHSQEKRMVHQKPPSSTQTKPSVPSAKPKKAQKKARATPHANKRKKKPPARSSQENDQKKQEQLAIEYSKMHDIWMSSKFQRFGQSSPRSFPVLLRNIPVFNQILKPVTQSKTPSQHNELFPPINQIKFTRNPELAKEKVKVEPSDSLPTCQFKTESGGQTFAEPADNSQGQPMVSVNQEAHPLPQSPPSNQCANIMAGAAQTQFHLGAQENLVHQIPPPTLPGTSPDTLLPDPASILRKGKVLHFDGITVVTEKREAQTSSNGPLGPTTDSAQSEFKESIMDLLSKPAKNLIAGLKEQEAAPCDCDGGTQKEKGPYYTHLGAGPSVAAVRELMETRFGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKLICLVRERVDHHCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGIDPKTCGASFSFGCSWSMYFNGCKFGRSENPRKFRLAPNYPLHNYYKRITGMSSEGSDVKTGWIIPDRKTLISREEKQLEKNLQELATVLAPLYKQMAPVAYQNQVEYEEVAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPEDEQLHVLPLYRLADTDEFGSVEGMKAKIKSGAIQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGTAGLRRKRISASPKGAPGSHNTKSFSSASSTSHLVKDESTDFCPLQASSAETSTCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGECTGTVQPAEVAAHPHQSLPTADSPVHAEPLTSPSEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEPQHPEDDNLPQLDEFWSDSEEIYADPSFGGVAIAPIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFYQHKSLNKPNHGFDINKIKCKCKKVTKKKPADRECPDVSPEANLSHQIPSRVASTLTRDNVVTVSPYSLTHVAGPYNRWV

Target ORF information:

RefSeq Version	XM_011243508.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243508.2

ORF Insert Sequence:

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG 
AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC 
CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT 
ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC 
GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG 
GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG 
CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA 
AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC 
CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT 
ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC 
TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA 
ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT 
CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC 
CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG 
CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT 
GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA 
GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC 
TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC 
AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA 
AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT 
TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC 
GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tet1 ( XM_006513869.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513932.1 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X2, mRNA.

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CloneID	OMu44647
Clone ID Related Accession (Same CDS sequence)	XM_006513867.3 , XM_011243508.2 , XM_006513867.4 , XM_006513869.3
Accession Version	XM_006513869.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methylcytosine dioxygenase TET1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513869.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG 
AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC 
CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT 
ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC 
GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG 
GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG 
CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA 
AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC 
CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT 
ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC 
TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA 
ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT 
CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC 
CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG 
CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT 
GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA 
GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC 
TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC 
AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA 
AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT 
TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC 
GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513932.1
CDS	278..6451
Translation	MSRSRPAKPSKSVKTKLQKKKDIQMKTKTSKQAVRHGASAKAVNPGKPKQLIKRRDGKKETEDKTPTPAPSFLTRAGAARMNRDRNQVLFQNPDSLTCNGFTMALRRTSLSWRLSQRPVVTPKPKKVPPSKKQCTHNIQDEPGVKHSENDSVPSQHATVSPGTENGEQNRCLVEGESQEITQSCPVFEERIEDTQSCISASGNLEAEISWPLEGTHCEELLSHQTSDNECTSPQECAPLPQRSTSEVTSQKNTSNQLADLSSQVESIKLSDPSPNPTGSDHNGFPDSSFRIVPELDLKTCMPLDESVYPTALIRFILAGSQPDVFDTKPQEKTLITTPEQVGSHPNQVLDATSVLGQAFSTLPLQWGFSGANLVQVEALGKGSDSPEDLGAITMLNQQETVAMDMDRNATPDLPIFLPKPPNTVATYSSPLLGPEPHSSTSCGLEVQGATPILTLDSGHTPQLPPNPESSSVPLVIAANGTRAEKQFGTSLFPAVPQGFTVAAENEVQHAPLDLTQGSQAAPSKLEGEISRVSITGSADVKATAMSMPVTQASTSSPPCNSTPPMVERRKRKACGVCEPCQQKANCGECTYCKNRKNSHQICKKRKCEVLKKKPEATSQAQVTKENKRPQREKKPKVLKTDFNNKPVNGPKSESMDCSRRGHGEEEQRLDLITHPLENVRKNAGGMTGIEVEKWAPNKKSHLAEGQVKGSCDANLTGVENPQPSEDDKQQTNPSPTFAQTIRNGMKNVHCLPTDTHLPLNKLNHEEFSKALGNNSSKLLTDPSNCKDAMSVTTSGGECDHLKGPRNTLLFQKPGLNCRSGAEPTIFNNHPNTHSAGSRPHPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSESSSPSKPEKDEEAHQKTASLLNSCKAILHSVRKDLQDPNVQGKGLHHDTVVFNGQNRTFKSPDSFATNQALIKSQGYPSSPTAEKKGAAGGRAPFDGFENSHPLPIESHNLENCSQVLSCDQNLSSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINAEVRNAESTPESLVAKNTKQKHSQEKRMVHQKPPSSTQTKPSVPSAKPKKAQKKARATPHANKRKKKPPARSSQENDQKKQEQLAIEYSKMHDIWMSSKFQRFGQSSPRSFPVLLRNIPVFNQILKPVTQSKTPSQHNELFPPINQIKFTRNPELAKEKVKVEPSDSLPTCQFKTESGGQTFAEPADNSQGQPMVSVNQEAHPLPQSPPSNQCANIMAGAAQTQFHLGAQENLVHQIPPPTLPGTSPDTLLPDPASILRKGKVLHFDGITVVTEKREAQTSSNGPLGPTTDSAQSEFKESIMDLLSKPAKNLIAGLKEQEAAPCDCDGGTQKEKGPYYTHLGAGPSVAAVRELMETRFGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKLICLVRERVDHHCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGIDPKTCGASFSFGCSWSMYFNGCKFGRSENPRKFRLAPNYPLHNYYKRITGMSSEGSDVKTGWIIPDRKTLISREEKQLEKNLQELATVLAPLYKQMAPVAYQNQVEYEEVAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPEDEQLHVLPLYRLADTDEFGSVEGMKAKIKSGAIQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGTAGLRRKRISASPKGAPGSHNTKSFSSASSTSHLVKDESTDFCPLQASSAETSTCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGECTGTVQPAEVAAHPHQSLPTADSPVHAEPLTSPSEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEPQHPEDDNLPQLDEFWSDSEEIYADPSFGGVAIAPIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFYQHKSLNKPNHGFDINKIKCKCKKVTKKKPADRECPDVSPEANLSHQIPSRVASTLTRDNVVTVSPYSLTHVAGPYNRWV

Target ORF information:

RefSeq Version	XM_006513869.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513869.3

ORF Insert Sequence:

ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG 
AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA 
ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA 
ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG 
TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC 
ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC 
GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC 
CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA 
ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA 
TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA 
CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC 
CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG 
AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC 
CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC 
ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA 
CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA 
GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT 
ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC 
AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT 
GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT 
CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC 
TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT 
CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT 
ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA 
GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT 
GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC 
AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT 
TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC 
CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG 
ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA 
CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA 
GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT 
GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG 
AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA 
CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC 
CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC 
ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC 
AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT 
GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT 
CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT 
GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC 
CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT 
CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG 
ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA 
GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG 
AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC 
TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT 
ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG 
AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT 
GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC 
CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA 
TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT 
GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA 
CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA 
AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT 
CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC 
ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT 
CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA 
CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT 
GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC 
AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC 
ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT 
GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG 
GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC 
CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG 
GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA 
CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA 
GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA 
AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT 
GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG 
GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC 
CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA 
TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA 
GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG 
AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG 
AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC 
CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT 
ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC 
GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG 
GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG 
CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA 
AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC 
CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT 
ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC 
TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA 
ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT 
CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC 
CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG 
CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT 
GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA 
GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC 
TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC 
AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA 
AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT 
TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC 
GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Tet1
Entrez Gene ID	52463
Full Name	tet methylcytosine dioxygenase 1
Synonyms	2510010B09Rik,AA517754,BB001228,Cxxc6,D10Ertd17e,LCX,mKIAA1676
General protein information	Preferred Names tet methylcytosine dioxygenase 1 Names methylcytosine dioxygenase TET1 CXXC finger 6 CXXC-type zinc finger protein 6 ten-eleven translocation 1 gene protein homolog tet oncogene 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 B4\|10 32.48 cM

Rattus norvegicus (Norway rat)	Tet1	XP_006256460.1
Pan troglodytes (chimpanzee)	TET1	XP_507822.3
Bos taurus (cattle)	TET1	XP_003588047.2
	TET1	XP_002805756.1
Canis lupus familiaris (dog)	TET1	XP_536371.2
Mus musculus (house mouse)	Tet1	NP_001240786.1
Homo sapiens (human)	TET1	NP_085128.2
Gallus gallus (chicken)	TET1	XP_004942068.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Tet1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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