Tet1 cDNA ORF clone, Mus musculus(house mouse)

The following Tet1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tet1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu77588 XM_030245174.1
Latest version!
Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu77588 XM_017314027.2
Latest version!
Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu85476 XM_030245175.1
Latest version!
Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu44647 XM_006513867.4
Latest version!
Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu20453 NM_027384.1
Latest version!
Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu20533 NM_001253857.2
Latest version!
Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu20533 XM_011243509.2
Latest version!
Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu44647 XM_011243508.2
Latest version!
Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu44647 XM_006513869.3
Latest version!
Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OMu44647 XM_006513867.3 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu77588 XM_017314027.1 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu20533 NM_001253857.1 Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu77588
    Clone ID Related Accession (Same CDS sequence) XM_017314027.1 , XM_017314027.2 , XM_030245174.1
    Accession Version XM_017314027.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product methylcytosine dioxygenase TET1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
    CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG
    GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT
    TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT
    CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC
    ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT
    TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC
    GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC
    TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC
    GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT
    CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG
    GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC
    AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG
    GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT
    CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC
    GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG
    AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC
    ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT
    TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA
    CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA
    AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC
    ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG
    CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA
    CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG
    TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT
    CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC
    GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA
    AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT
    CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT
    AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC
    CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA
    GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT
    GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT
    GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT
    CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG
    CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT
    GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG
    GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT
    GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG
    TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA
    GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT
    CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA
    CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC
    GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC
    GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC
    TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC
    GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG
    AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT
    TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG
    GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC
    ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT
    GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC
    ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA
    GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG
    AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTA CTGCTGGTCT CAGAAGGAAG
    AGGATCTCTG CCAGCCCAAA GGGAGCCCCA GGTTCACACA ACACTAAGAG CTTTTCATCA
    GCCTCATCTA CTTCTCACCT AGTGAAAGAC GAATCTACAG ACTTCTGTCC CCTGCAGGCT
    TCCTCCGCAG AAACATCTAC CTGTACGTAC AGTAAAACAG CCTCAGGTGG GTTTGCAGAA
    ACAAGTAGTA TTCTCCACTG CACAATGCCT TCTGGAGCAC ACAGTGGTGC TAATGCAGCT
    GCTGGGGAAT GTACTGGAAC GGTGCAGCCT GCCGAGGTGG CTGCTCATCC TCACCAGTCT
    CTTCCCACAG CCGATTCTCC CGTTCATGCT GAGCCTCTCA CTAGTCCATC TGAGCAGCTA
    ACTTCTAACC AGTCAAACCA GCAGCTCCCT CTCCTCAGCA ATTCTCAGAA ACTGGCTTCC
    TGTCAGGTGG AAGATGAGCG GCACCCTGAA GCGGATGAGC CTCAGCACCC CGAGGACGAT
    AACTTGCCTC AACTTGATGA ATTCTGGTCA GACAGTGAGG AGATCTACGC CGATCCTTCC
    TTTGGTGGCG TGGCGATAGC ACCCATTCAC GGCTCGGTGC TCATTGAGTG CGCTCGGAAG
    GAGCTTCATG CTACCACCTC TTTGCGCTCC CCCAAACGAG GGGTCCCTTT TCGTGTGTCC
    CTTGTATTCT ACCAGCACAA AAGCCTAAAC AAGCCTAATC ATGGTTTTGA TATCAACAAA
    ATTAAGTGTA AATGCAAAAA AGTAACGAAA AAAAAGCCCG CAGACCGGGA GTGTCCTGAT
    GTATCCCCCG AAGCCAATTT ATCACACCAA ATTCCTTCTC GAGTTGCATC AACCTTAACC
    CGAGACAATG TTGTTACCGT GTCCCCATAC TCTCTCACTC ATGTTGCGGG ACCCTACAAT
    CGTTGGGTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017169516.1
    CDS1138..5349
    Translation

    Target ORF information:

    RefSeq Version XM_017314027.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314027.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
    CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG
    GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT
    TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT
    CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC
    ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT
    TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC
    GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC
    TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC
    GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT
    CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG
    GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC
    AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG
    GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT
    CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC
    GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG
    AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC
    ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT
    TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA
    CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA
    AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC
    ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG
    CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA
    CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG
    TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT
    CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC
    GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA
    AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT
    CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT
    AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC
    CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA
    GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT
    GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT
    GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT
    CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG
    CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT
    GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG
    GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT
    GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG
    TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA
    GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT
    CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA
    CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC
    GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC
    GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC
    TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC
    GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG
    AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT
    TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG
    GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC
    ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT
    GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC
    ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA
    GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG
    AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTA CTGCTGGTCT CAGAAGGAAG
    AGGATCTCTG CCAGCCCAAA GGGAGCCCCA GGTTCACACA ACACTAAGAG CTTTTCATCA
    GCCTCATCTA CTTCTCACCT AGTGAAAGAC GAATCTACAG ACTTCTGTCC CCTGCAGGCT
    TCCTCCGCAG AAACATCTAC CTGTACGTAC AGTAAAACAG CCTCAGGTGG GTTTGCAGAA
    ACAAGTAGTA TTCTCCACTG CACAATGCCT TCTGGAGCAC ACAGTGGTGC TAATGCAGCT
    GCTGGGGAAT GTACTGGAAC GGTGCAGCCT GCCGAGGTGG CTGCTCATCC TCACCAGTCT
    CTTCCCACAG CCGATTCTCC CGTTCATGCT GAGCCTCTCA CTAGTCCATC TGAGCAGCTA
    ACTTCTAACC AGTCAAACCA GCAGCTCCCT CTCCTCAGCA ATTCTCAGAA ACTGGCTTCC
    TGTCAGGTGG AAGATGAGCG GCACCCTGAA GCGGATGAGC CTCAGCACCC CGAGGACGAT
    AACTTGCCTC AACTTGATGA ATTCTGGTCA GACAGTGAGG AGATCTACGC CGATCCTTCC
    TTTGGTGGCG TGGCGATAGC ACCCATTCAC GGCTCGGTGC TCATTGAGTG CGCTCGGAAG
    GAGCTTCATG CTACCACCTC TTTGCGCTCC CCCAAACGAG GGGTCCCTTT TCGTGTGTCC
    CTTGTATTCT ACCAGCACAA AAGCCTAAAC AAGCCTAATC ATGGTTTTGA TATCAACAAA
    ATTAAGTGTA AATGCAAAAA AGTAACGAAA AAAAAGCCCG CAGACCGGGA GTGTCCTGAT
    GTATCCCCCG AAGCCAATTT ATCACACCAA ATTCCTTCTC GAGTTGCATC AACCTTAACC
    CGAGACAATG TTGTTACCGT GTCCCCATAC TCTCTCACTC ATGTTGCGGG ACCCTACAAT
    CGTTGGGTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77588
    Clone ID Related Accession (Same CDS sequence) XM_017314027.1 , XM_017314027.2 , XM_030245174.1
    Accession Version XM_017314027.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product methylcytosine dioxygenase TET1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314027.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
    CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG
    GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT
    TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT
    CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC
    ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT
    TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC
    GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC
    TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC
    GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT
    CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG
    GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC
    AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG
    GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT
    CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC
    GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG
    AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC
    ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT
    TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA
    CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA
    AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC
    ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG
    CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA
    CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG
    TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT
    CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC
    GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA
    AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT
    CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT
    AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC
    CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA
    GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT
    GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT
    GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT
    CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG
    CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT
    GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG
    GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT
    GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG
    TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA
    GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT
    CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA
    CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC
    GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC
    GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC
    TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC
    GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG
    AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT
    TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG
    GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC
    ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT
    GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC
    ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA
    GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG
    AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTA CTGCTGGTCT CAGAAGGAAG
    AGGATCTCTG CCAGCCCAAA GGGAGCCCCA GGTTCACACA ACACTAAGAG CTTTTCATCA
    GCCTCATCTA CTTCTCACCT AGTGAAAGAC GAATCTACAG ACTTCTGTCC CCTGCAGGCT
    TCCTCCGCAG AAACATCTAC CTGTACGTAC AGTAAAACAG CCTCAGGTGG GTTTGCAGAA
    ACAAGTAGTA TTCTCCACTG CACAATGCCT TCTGGAGCAC ACAGTGGTGC TAATGCAGCT
    GCTGGGGAAT GTACTGGAAC GGTGCAGCCT GCCGAGGTGG CTGCTCATCC TCACCAGTCT
    CTTCCCACAG CCGATTCTCC CGTTCATGCT GAGCCTCTCA CTAGTCCATC TGAGCAGCTA
    ACTTCTAACC AGTCAAACCA GCAGCTCCCT CTCCTCAGCA ATTCTCAGAA ACTGGCTTCC
    TGTCAGGTGG AAGATGAGCG GCACCCTGAA GCGGATGAGC CTCAGCACCC CGAGGACGAT
    AACTTGCCTC AACTTGATGA ATTCTGGTCA GACAGTGAGG AGATCTACGC CGATCCTTCC
    TTTGGTGGCG TGGCGATAGC ACCCATTCAC GGCTCGGTGC TCATTGAGTG CGCTCGGAAG
    GAGCTTCATG CTACCACCTC TTTGCGCTCC CCCAAACGAG GGGTCCCTTT TCGTGTGTCC
    CTTGTATTCT ACCAGCACAA AAGCCTAAAC AAGCCTAATC ATGGTTTTGA TATCAACAAA
    ATTAAGTGTA AATGCAAAAA AGTAACGAAA AAAAAGCCCG CAGACCGGGA GTGTCCTGAT
    GTATCCCCCG AAGCCAATTT ATCACACCAA ATTCCTTCTC GAGTTGCATC AACCTTAACC
    CGAGACAATG TTGTTACCGT GTCCCCATAC TCTCTCACTC ATGTTGCGGG ACCCTACAAT
    CGTTGGGTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017169516.1
    CDS3919..8130
    Translation

    Target ORF information:

    RefSeq Version XM_017314027.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314027.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
    CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG
    GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT
    TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT
    CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC
    ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT
    TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC
    GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC
    TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC
    GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT
    CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG
    GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC
    AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG
    GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT
    CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC
    GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG
    AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC
    ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT
    TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA
    CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA
    AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC
    ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG
    CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA
    CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG
    TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT
    CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC
    GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA
    AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT
    CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT
    AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC
    CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA
    GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT
    GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT
    GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT
    CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG
    CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT
    GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG
    GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT
    GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG
    TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA
    GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT
    CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA
    CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC
    GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC
    GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC
    TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC
    GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG
    AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT
    TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG
    GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC
    ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT
    GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC
    ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA
    GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG
    AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTA CTGCTGGTCT CAGAAGGAAG
    AGGATCTCTG CCAGCCCAAA GGGAGCCCCA GGTTCACACA ACACTAAGAG CTTTTCATCA
    GCCTCATCTA CTTCTCACCT AGTGAAAGAC GAATCTACAG ACTTCTGTCC CCTGCAGGCT
    TCCTCCGCAG AAACATCTAC CTGTACGTAC AGTAAAACAG CCTCAGGTGG GTTTGCAGAA
    ACAAGTAGTA TTCTCCACTG CACAATGCCT TCTGGAGCAC ACAGTGGTGC TAATGCAGCT
    GCTGGGGAAT GTACTGGAAC GGTGCAGCCT GCCGAGGTGG CTGCTCATCC TCACCAGTCT
    CTTCCCACAG CCGATTCTCC CGTTCATGCT GAGCCTCTCA CTAGTCCATC TGAGCAGCTA
    ACTTCTAACC AGTCAAACCA GCAGCTCCCT CTCCTCAGCA ATTCTCAGAA ACTGGCTTCC
    TGTCAGGTGG AAGATGAGCG GCACCCTGAA GCGGATGAGC CTCAGCACCC CGAGGACGAT
    AACTTGCCTC AACTTGATGA ATTCTGGTCA GACAGTGAGG AGATCTACGC CGATCCTTCC
    TTTGGTGGCG TGGCGATAGC ACCCATTCAC GGCTCGGTGC TCATTGAGTG CGCTCGGAAG
    GAGCTTCATG CTACCACCTC TTTGCGCTCC CCCAAACGAG GGGTCCCTTT TCGTGTGTCC
    CTTGTATTCT ACCAGCACAA AAGCCTAAAC AAGCCTAATC ATGGTTTTGA TATCAACAAA
    ATTAAGTGTA AATGCAAAAA AGTAACGAAA AAAAAGCCCG CAGACCGGGA GTGTCCTGAT
    GTATCCCCCG AAGCCAATTT ATCACACCAA ATTCCTTCTC GAGTTGCATC AACCTTAACC
    CGAGACAATG TTGTTACCGT GTCCCCATAC TCTCTCACTC ATGTTGCGGG ACCCTACAAT
    CGTTGGGTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77588
    Clone ID Related Accession (Same CDS sequence) XM_017314027.1 , XM_017314027.2 , XM_030245174.1
    Accession Version XM_030245174.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product methylcytosine dioxygenase TET1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
    CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG
    GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT
    TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT
    CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC
    ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT
    TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC
    GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC
    TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC
    GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT
    CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG
    GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC
    AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG
    GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT
    CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC
    GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG
    AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC
    ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT
    TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA
    CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA
    AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC
    ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG
    CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA
    CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG
    TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT
    CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC
    GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA
    AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT
    CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT
    AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC
    CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA
    GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT
    GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT
    GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT
    CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG
    CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT
    GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG
    GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT
    GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG
    TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA
    GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT
    CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA
    CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC
    GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC
    GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC
    TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC
    GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG
    AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT
    TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG
    GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC
    ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT
    GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC
    ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA
    GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG
    AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTA CTGCTGGTCT CAGAAGGAAG
    AGGATCTCTG CCAGCCCAAA GGGAGCCCCA GGTTCACACA ACACTAAGAG CTTTTCATCA
    GCCTCATCTA CTTCTCACCT AGTGAAAGAC GAATCTACAG ACTTCTGTCC CCTGCAGGCT
    TCCTCCGCAG AAACATCTAC CTGTACGTAC AGTAAAACAG CCTCAGGTGG GTTTGCAGAA
    ACAAGTAGTA TTCTCCACTG CACAATGCCT TCTGGAGCAC ACAGTGGTGC TAATGCAGCT
    GCTGGGGAAT GTACTGGAAC GGTGCAGCCT GCCGAGGTGG CTGCTCATCC TCACCAGTCT
    CTTCCCACAG CCGATTCTCC CGTTCATGCT GAGCCTCTCA CTAGTCCATC TGAGCAGCTA
    ACTTCTAACC AGTCAAACCA GCAGCTCCCT CTCCTCAGCA ATTCTCAGAA ACTGGCTTCC
    TGTCAGGTGG AAGATGAGCG GCACCCTGAA GCGGATGAGC CTCAGCACCC CGAGGACGAT
    AACTTGCCTC AACTTGATGA ATTCTGGTCA GACAGTGAGG AGATCTACGC CGATCCTTCC
    TTTGGTGGCG TGGCGATAGC ACCCATTCAC GGCTCGGTGC TCATTGAGTG CGCTCGGAAG
    GAGCTTCATG CTACCACCTC TTTGCGCTCC CCCAAACGAG GGGTCCCTTT TCGTGTGTCC
    CTTGTATTCT ACCAGCACAA AAGCCTAAAC AAGCCTAATC ATGGTTTTGA TATCAACAAA
    ATTAAGTGTA AATGCAAAAA AGTAACGAAA AAAAAGCCCG CAGACCGGGA GTGTCCTGAT
    GTATCCCCCG AAGCCAATTT ATCACACCAA ATTCCTTCTC GAGTTGCATC AACCTTAACC
    CGAGACAATG TTGTTACCGT GTCCCCATAC TCTCTCACTC ATGTTGCGGG ACCCTACAAT
    CGTTGGGTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101034.1
    CDS3572..7783
    Translation

    Target ORF information:

    RefSeq Version XM_030245174.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245174.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
    CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG
    GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT
    TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT
    CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC
    ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT
    TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC
    GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC
    TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC
    GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT
    CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG
    GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC
    AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG
    GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT
    CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC
    GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG
    AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC
    ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT
    TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA
    CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA
    AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC
    ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG
    CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA
    CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG
    TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT
    CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC
    GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA
    AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT
    CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT
    AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC
    CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA
    GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT
    GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT
    GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT
    CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG
    CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT
    GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG
    GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT
    GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG
    TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA
    GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT
    CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA
    CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC
    GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC
    GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC
    TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC
    GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG
    AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT
    TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG
    GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC
    ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT
    GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC
    ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA
    GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG
    AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTA CTGCTGGTCT CAGAAGGAAG
    AGGATCTCTG CCAGCCCAAA GGGAGCCCCA GGTTCACACA ACACTAAGAG CTTTTCATCA
    GCCTCATCTA CTTCTCACCT AGTGAAAGAC GAATCTACAG ACTTCTGTCC CCTGCAGGCT
    TCCTCCGCAG AAACATCTAC CTGTACGTAC AGTAAAACAG CCTCAGGTGG GTTTGCAGAA
    ACAAGTAGTA TTCTCCACTG CACAATGCCT TCTGGAGCAC ACAGTGGTGC TAATGCAGCT
    GCTGGGGAAT GTACTGGAAC GGTGCAGCCT GCCGAGGTGG CTGCTCATCC TCACCAGTCT
    CTTCCCACAG CCGATTCTCC CGTTCATGCT GAGCCTCTCA CTAGTCCATC TGAGCAGCTA
    ACTTCTAACC AGTCAAACCA GCAGCTCCCT CTCCTCAGCA ATTCTCAGAA ACTGGCTTCC
    TGTCAGGTGG AAGATGAGCG GCACCCTGAA GCGGATGAGC CTCAGCACCC CGAGGACGAT
    AACTTGCCTC AACTTGATGA ATTCTGGTCA GACAGTGAGG AGATCTACGC CGATCCTTCC
    TTTGGTGGCG TGGCGATAGC ACCCATTCAC GGCTCGGTGC TCATTGAGTG CGCTCGGAAG
    GAGCTTCATG CTACCACCTC TTTGCGCTCC CCCAAACGAG GGGTCCCTTT TCGTGTGTCC
    CTTGTATTCT ACCAGCACAA AAGCCTAAAC AAGCCTAATC ATGGTTTTGA TATCAACAAA
    ATTAAGTGTA AATGCAAAAA AGTAACGAAA AAAAAGCCCG CAGACCGGGA GTGTCCTGAT
    GTATCCCCCG AAGCCAATTT ATCACACCAA ATTCCTTCTC GAGTTGCATC AACCTTAACC
    CGAGACAATG TTGTTACCGT GTCCCCATAC TCTCTCACTC ATGTTGCGGG ACCCTACAAT
    CGTTGGGTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85476
    Clone ID Related Accession (Same CDS sequence) XM_030245175.1
    Accession Version XM_030245175.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product methylcytosine dioxygenase TET1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
    CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG
    GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT
    TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT
    CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC
    ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT
    TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC
    GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC
    TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC
    GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT
    CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG
    GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC
    AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG
    GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT
    CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC
    GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG
    AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC
    ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT
    TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA
    CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA
    AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC
    ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG
    CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA
    CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG
    TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT
    CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC
    GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA
    AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT
    CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT
    AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC
    CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA
    GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT
    GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT
    GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT
    CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG
    CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT
    GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG
    GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT
    GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG
    TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA
    GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT
    CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA
    CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC
    GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC
    GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC
    TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC
    GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG
    AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT
    TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG
    GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC
    ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT
    GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC
    ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA
    GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG
    AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTT CACACAACAC TAAGAGCTTT
    TCATCAGCCT CATCTACTTC TCACCTAGTG AAAGACGAAT CTACAGACTT CTGTCCCCTG
    CAGGCTTCCT CCGCAGAAAC ATCTACCTGT ACGTACAGTA AAACAGCCTC AGGTGGGTTT
    GCAGAAACAA GTAGTATTCT CCACTGCACA ATGCCTTCTG GAGCACACAG TGGTGCTAAT
    GCAGCTGCTG GGGAATGTAC TGGAACGGTG CAGCCTGCCG AGGTGGCTGC TCATCCTCAC
    CAGTCTCTTC CCACAGCCGA TTCTCCCGTT CATGCTGAGC CTCTCACTAG TCCATCTGAG
    CAGCTAACTT CTAACCAGTC AAACCAGCAG CTCCCTCTCC TCAGCAATTC TCAGAAACTG
    GCTTCCTGTC AGGTGGAAGA TGAGCGGCAC CCTGAAGCGG ATGAGCCTCA GCACCCCGAG
    GACGATAACT TGCCTCAACT TGATGAATTC TGGTCAGACA GTGAGGAGAT CTACGCCGAT
    CCTTCCTTTG GTGGCGTGGC GATAGCACCC ATTCACGGCT CGGTGCTCAT TGAGTGCGCT
    CGGAAGGAGC TTCATGCTAC CACCTCTTTG CGCTCCCCCA AACGAGGGGT CCCTTTTCGT
    GTGTCCCTTG TATTCTACCA GCACAAAAGC CTAAACAAGC CTAATCATGG TTTTGATATC
    AACAAAATTA AGTGTAAATG CAAAAAAGTA ACGAAAAAAA AGCCCGCAGA CCGGGAGTGT
    CCTGATGTAT CCCCCGAAGC CAATTTATCA CACCAAATTC CTTCTCGAGT TGCATCAACC
    TTAACCCGAG ACAATGTTGT TACCGTGTCC CCATACTCTC TCACTCATGT TGCGGGACCC
    TACAATCGTT GGGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101035.1
    CDS3919..8076
    Translation

    Target ORF information:

    RefSeq Version XM_030245175.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245175.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACTGCA GTAGACGTGG TCATGGGGAA GAAGAGCAAA GGTTGGATTT GATCACACAT 
    CCCCTTGAAA ATGTAAGGAA GAATGCAGGC GGCATGACGG GAATTGAGGT GGAGAAGTGG
    GCGCCAAACA AGAAATCACA CTTAGCAGAG GGTCAAGTCA AAGGAAGCTG TGATGCCAAT
    TTAACTGGGG TGGAAAACCC CCAGCCCTCT GAAGATGACA AGCAGCAAAC CAACCCTTCT
    CCGACATTTG CCCAGACCAT AAGGAACGGC ATGAAAAATG TACACTGTTT ACCCACTGAC
    ACACACCTTC CGCTCAACAA ATTGAATCAT GAGGAATTTA GCAAGGCGTT GGGAAATAAT
    TCCTCTAAAT TGCTGACTGA TCCCTCAAAC TGCAAGGATG CCATGAGTGT CACCACCTCC
    GGGGGGGAGT GTGATCATCT CAAGGGGCCA AGGAACACCC TGCTCTTCCA GAAGCCTGGC
    TTGAACTGCA GGTCTGGTGC AGAACCCACC ATCTTTAATA ATCACCCTAA TACACACAGC
    GCTGGGAGTC GACCTCACCC TCCTGAGAAG GTACCCAACA AAGAACCCAA AGATGGCTCT
    CCGGTTCAGC CAAGTCTTCT ATCCCTAATG AAGGACAGGA GATTAACGCT GGAACAAGTG
    GTAGCCATAG AAGCCCTGAC TCAGCTCTCA GAAGCGCCCT CAGAGAGCTC CTCCCCATCC
    AAGCCGGAGA AGGATGAAGA AGCGCATCAG AAAACGGCTA GTCTGCTTAA CAGTTGCAAG
    GCTATCCTTC ATTCGGTGAG AAAAGACCTC CAAGACCCAA ACGTTCAAGG AAAGGGCCTT
    CACCATGACA CTGTTGTCTT TAATGGCCAG AACAGAACAT TCAAGTCACC TGATAGTTTC
    GCTACTAACC AAGCACTTAT AAAATCACAA GGATATCCAA GTTCACCTAC AGCTGAGAAG
    AAAGGCGCTG CTGGTGGGAG AGCTCCCTTT GATGGTTTTG AGAATTCACA TCCATTGCCC
    ATAGAAAGTC ACAATCTTGA AAACTGCAGT CAGGTACTAA GCTGCGATCA GAATTTGAGT
    TCCCATGACC CATCATGCCA GGATGCACCC TACAGTCAAA TTGAAGAAGA TGTCGCAGCA
    CAATTGACCC AACTTGCATC CACGATTAAT CACATCAACG CCGAGGTCAG GAATGCTGAA
    AGTACACCAG AAAGCCTTGT TGCAAAGAAC ACAAAGCAAA AACACAGTCA GGAGAAACGC
    ATGGTACATC AGAAGCCACC CTCTAGTACA CAAACTAAAC CCAGTGTACC ATCAGCAAAG
    CCAAAGAAGG CCCAGAAAAA GGCAAGAGCG ACCCCACACG CAAACAAGCG GAAAAAGAAA
    CCCCCAGCCC GAAGCTCTCA AGAAAATGAT CAGAAGAAGC AAGAGCAGCT AGCTATAGAG
    TATAGTAAAA TGCATGACAT TTGGATGTCA TCTAAGTTTC AAAGGTTTGG CCAGTCTAGT
    CCACGTAGTT TTCCTGTTCT GTTGAGAAAT ATTCCTGTCT TTAACCAAAT ATTAAAGCCC
    GTGACTCAGA GTAAAACACC CTCACAACAT AATGAGTTAT TTCCTCCTAT CAATCAGATA
    AAATTCACAA GAAACCCTGA ATTGGCAAAG GAGAAAGTGA AGGTTGAACC ATCAGATTCT
    CTGCCAACAT GCCAATTCAA GACAGAATCC GGTGGGCAGA CATTTGCAGA GCCGGCAGAT
    AATTCTCAGG GACAGCCCAT GGTGAGTGTC AATCAGGAAG CTCACCCTCT GCCCCAGTCC
    CCACCATCTA ACCAGTGTGC TAATATAATG GCTGGTGCTG CCCAAACACA GTTTCATCTA
    GGCGCTCAAG AGAACCTGGT GCATCAGATA CCACCACCGA CGTTGCCTGG TACCTCCCCT
    GACACACTCT TGCCAGACCC AGCATCAATC CTCAGGAAAG GGAAAGTGTT ACATTTCGAT
    GGAATTACAG TTGTTACGGA GAAGCGTGAA GCTCAAACAT CAAGCAATGG ACCACTGGGT
    CCCACCACAG ACAGTGCACA GTCAGAATTT AAAGAGAGCA TCATGGACTT ACTTAGCAAG
    CCTGCAAAAA ACCTGATAGC TGGATTGAAG GAACAGGAAG CTGCACCCTG TGACTGTGAT
    GGAGGTACAC AAAAAGAAAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTG
    GCTGCTGTCA GGGAGCTCAT GGAGACTAGG TTTGGCCAGA AGGGGAAGGC AATCCGGATT
    GAGAAGATAG TGTTCACGGG GAAGGAAGGG AAGAGCTCTC AGGGCTGCCC GGTCGCCAAG
    TGGGTGATCA GAAGAAGTGG TCCTGAAGAG AAGCTTATTT GTTTGGTTCG TGAGCGTGTA
    GACCATCACT GTTCGACGGC TGTGATAGTT GTCCTTATCC TGCTGTGGGA AGGTATCCCT
    CGCCTGATGG CTGACCGCCT GTACAAAGAG CTCACTGAGA ACTTGAGGTC CTACAGCGGA
    CATCCCACAG ACCGAAGATG TACCCTCAAC AAAAAGCGTA CCTGCACCTG TCAAGGCATC
    GACCCAAAAA CCTGCGGAGC GTCCTTCTCC TTTGGCTGTT CGTGGAGCAT GTATTTCAAC
    GGCTGTAAGT TTGGGAGGAG TGAAAACCCC AGAAAATTCA GACTTGCTCC AAACTACCCC
    TTACATAACT ACTATAAGAG AATTACTGGA ATGAGTTCTG AAGGAAGTGA CGTGAAAACC
    GGGTGGATCA TTCCAGACCG CAAGACCCTC ATAAGCAGAG AGGAAAAACA GCTTGAAAAG
    AATTTACAAG AATTGGCTAC AGTATTAGCT CCACTTTACA AGCAGATGGC TCCAGTTGCT
    TATCAAAATC AGGTGGAATA TGAAGAAGTT GCTGGAGACT GTCGACTTGG AAATGAAGAG
    GGGCGTCCTT TCTCTGGTGT CACCTGTTGC ATGGATTTTT GTGCCCATTC TCACAAGGAC
    ATTCACAACA TGCACAACGG AAGCACCGTG GTGTGTACGT TGATTCGAGC AGATGGCCGT
    GACACAAATT GTCCCGAGGA TGAACAACTC CACGTCCTGC CACTATACCG GCTTGCAGAC
    ACTGATGAAT TTGGCTCCGT GGAAGGGATG AAGGCCAAAA TCAAATCTGG GGCCATCCAA
    GTCAATGGGC CAACCAGGAA GAGGCGACTA CGTTTTACTG AGCCTGTTCC TCGATGTGGG
    AAGAGGGCCA AAATGAAGCA GAACCACAAT AAATCAGGTT CACACAACAC TAAGAGCTTT
    TCATCAGCCT CATCTACTTC TCACCTAGTG AAAGACGAAT CTACAGACTT CTGTCCCCTG
    CAGGCTTCCT CCGCAGAAAC ATCTACCTGT ACGTACAGTA AAACAGCCTC AGGTGGGTTT
    GCAGAAACAA GTAGTATTCT CCACTGCACA ATGCCTTCTG GAGCACACAG TGGTGCTAAT
    GCAGCTGCTG GGGAATGTAC TGGAACGGTG CAGCCTGCCG AGGTGGCTGC TCATCCTCAC
    CAGTCTCTTC CCACAGCCGA TTCTCCCGTT CATGCTGAGC CTCTCACTAG TCCATCTGAG
    CAGCTAACTT CTAACCAGTC AAACCAGCAG CTCCCTCTCC TCAGCAATTC TCAGAAACTG
    GCTTCCTGTC AGGTGGAAGA TGAGCGGCAC CCTGAAGCGG ATGAGCCTCA GCACCCCGAG
    GACGATAACT TGCCTCAACT TGATGAATTC TGGTCAGACA GTGAGGAGAT CTACGCCGAT
    CCTTCCTTTG GTGGCGTGGC GATAGCACCC ATTCACGGCT CGGTGCTCAT TGAGTGCGCT
    CGGAAGGAGC TTCATGCTAC CACCTCTTTG CGCTCCCCCA AACGAGGGGT CCCTTTTCGT
    GTGTCCCTTG TATTCTACCA GCACAAAAGC CTAAACAAGC CTAATCATGG TTTTGATATC
    AACAAAATTA AGTGTAAATG CAAAAAAGTA ACGAAAAAAA AGCCCGCAGA CCGGGAGTGT
    CCTGATGTAT CCCCCGAAGC CAATTTATCA CACCAAATTC CTTCTCGAGT TGCATCAACC
    TTAACCCGAG ACAATGTTGT TACCGTGTCC CCATACTCTC TCACTCATGT TGCGGGACCC
    TACAATCGTT GGGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu44647
    Clone ID Related Accession (Same CDS sequence) XM_006513867.3 , XM_011243508.2 , XM_006513867.4 , XM_006513869.3
    Accession Version XM_006513867.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product methylcytosine dioxygenase TET1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513867.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG
    AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC
    CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT
    ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA
    TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT
    GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC
    GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG
    GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC
    GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG
    AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG
    CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA
    AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC
    CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT
    ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC
    TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA
    ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT
    CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC
    CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG
    CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT
    GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA
    GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC
    TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC
    AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA
    AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT
    TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC
    GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006513930.1
    CDS413..6586
    Translation

    Target ORF information:

    RefSeq Version XM_006513867.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006513867.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG
    AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC
    CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT
    ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA
    TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT
    GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC
    GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG
    GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC
    GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG
    AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG
    CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA
    AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC
    CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT
    ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC
    TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA
    ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT
    CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC
    CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG
    CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT
    GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA
    GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC
    TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC
    AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA
    AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT
    TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC
    GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20453
    Clone ID Related Accession (Same CDS sequence) NM_027384.1
    Accession Version NM_027384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6024bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-22
    Organism Mus musculus(House mouse)
    Product methylcytosine dioxygenase TET1 isoform 2
    Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166359.3, CF897091.1, AK141438.1, AI605989.1 and AK010953.1. On or before Jun 25, 2009 this sequence version replaced XM_907080.3, XM_125673.7. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849381 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATGA AAAACAGCTT
    GAAAAGAATT TACAAGAATT GGCTACAGTA TTAGCTCCAC TTTACAAGCA GATGGCTCCA
    GTTGCTTATC AAAATCAGGT GGAATATGAA GAAGTTGCTG GAGACTGTCG ACTTGGAAAT
    GAAGAGGGGC GTCCTTTCTC TGGTGTCACC TGTTGCATGG ATTTTTGTGC CCATTCTCAC
    AAGGACATTC ACAACATGCA CAACGGAAGC ACCGTGGTGT GTACGTTGAT TCGAGCAGAT
    GGCCGTGACA CAAATTGTCC CGAGGATGAA CAACTCCACG TCCTGCCACT ATACCGGCTT
    GCAGACACTG ATGAATTTGG CTCCGTGGAA GGGATGAAGG CCAAAATCAA ATCTGGGGCC
    ATCCAAGTCA ATGGGCCAAC CAGGAAGAGG CGACTACGTT TTACTGAGCC TGTTCCTCGA
    TGTGGGAAGA GGGCCAAAAT GAAGCAGAAC CACAATAAAT CAGGTTCACA CAACACTAAG
    AGCTTTTCAT CAGCCTCATC TACTTCTCAC CTAGTGAAAG ACGAATCTAC AGACTTCTGT
    CCCCTGCAGG CTTCCTCCGC AGAAACATCT ACCTGTACGT ACAGTAAAAC AGCCTCAGGT
    GGGTTTGCAG AAACAAGTAG TATTCTCCAC TGCACAATGC CTTCTGGAGC ACACAGTGGT
    GCTAATGCAG CTGCTGGGGA ATGTACTGGA ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT
    CCTCACCAGT CTCTTCCCAC AGCCGATTCT CCCGTTCATG CTGAGCCTCT CACTAGTCCA
    TCTGAGCAGC TAACTTCTAA CCAGTCAAAC CAGCAGCTCC CTCTCCTCAG CAATTCTCAG
    AAACTGGCTT CCTGTCAGGT GGAAGATGAG CGGCACCCTG AAGCGGATGA GCCTCAGCAC
    CCCGAGGACG ATAACTTGCC TCAACTTGAT GAATTCTGGT CAGACAGTGA GGAGATCTAC
    GCCGATCCTT CCTTTGGTGG CGTGGCGATA GCACCCATTC ACGGCTCGGT GCTCATTGAG
    TGCGCTCGGA AGGAGCTTCA TGCTACCACC TCTTTGCGCT CCCCCAAACG AGGGGTCCCT
    TTTCGTGTGT CCCTTGTATT CTACCAGCAC AAAAGCCTAA ACAAGCCTAA TCATGGTTTT
    GATATCAACA AAATTAAGTG TAAATGCAAA AAAGTAACGA AAAAAAAGCC CGCAGACCGG
    GAGTGTCCTG ATGTATCCCC CGAAGCCAAT TTATCACACC AAATTCCTTC TCGAGTTGCA
    TCAACCTTAA CCCGAGACAA TGTTGTTACC GTGTCCCCAT ACTCTCTCAC TCATGTTGCG
    GGACCCTACA ATCGTTGGGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_081660.1
    CDS1..6024
    Translation

    Target ORF information:

    RefSeq Version NM_027384.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_027384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATGA AAAACAGCTT
    GAAAAGAATT TACAAGAATT GGCTACAGTA TTAGCTCCAC TTTACAAGCA GATGGCTCCA
    GTTGCTTATC AAAATCAGGT GGAATATGAA GAAGTTGCTG GAGACTGTCG ACTTGGAAAT
    GAAGAGGGGC GTCCTTTCTC TGGTGTCACC TGTTGCATGG ATTTTTGTGC CCATTCTCAC
    AAGGACATTC ACAACATGCA CAACGGAAGC ACCGTGGTGT GTACGTTGAT TCGAGCAGAT
    GGCCGTGACA CAAATTGTCC CGAGGATGAA CAACTCCACG TCCTGCCACT ATACCGGCTT
    GCAGACACTG ATGAATTTGG CTCCGTGGAA GGGATGAAGG CCAAAATCAA ATCTGGGGCC
    ATCCAAGTCA ATGGGCCAAC CAGGAAGAGG CGACTACGTT TTACTGAGCC TGTTCCTCGA
    TGTGGGAAGA GGGCCAAAAT GAAGCAGAAC CACAATAAAT CAGGTTCACA CAACACTAAG
    AGCTTTTCAT CAGCCTCATC TACTTCTCAC CTAGTGAAAG ACGAATCTAC AGACTTCTGT
    CCCCTGCAGG CTTCCTCCGC AGAAACATCT ACCTGTACGT ACAGTAAAAC AGCCTCAGGT
    GGGTTTGCAG AAACAAGTAG TATTCTCCAC TGCACAATGC CTTCTGGAGC ACACAGTGGT
    GCTAATGCAG CTGCTGGGGA ATGTACTGGA ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT
    CCTCACCAGT CTCTTCCCAC AGCCGATTCT CCCGTTCATG CTGAGCCTCT CACTAGTCCA
    TCTGAGCAGC TAACTTCTAA CCAGTCAAAC CAGCAGCTCC CTCTCCTCAG CAATTCTCAG
    AAACTGGCTT CCTGTCAGGT GGAAGATGAG CGGCACCCTG AAGCGGATGA GCCTCAGCAC
    CCCGAGGACG ATAACTTGCC TCAACTTGAT GAATTCTGGT CAGACAGTGA GGAGATCTAC
    GCCGATCCTT CCTTTGGTGG CGTGGCGATA GCACCCATTC ACGGCTCGGT GCTCATTGAG
    TGCGCTCGGA AGGAGCTTCA TGCTACCACC TCTTTGCGCT CCCCCAAACG AGGGGTCCCT
    TTTCGTGTGT CCCTTGTATT CTACCAGCAC AAAAGCCTAA ACAAGCCTAA TCATGGTTTT
    GATATCAACA AAATTAAGTG TAAATGCAAA AAAGTAACGA AAAAAAAGCC CGCAGACCGG
    GAGTGTCCTG ATGTATCCCC CGAAGCCAAT TTATCACACC AAATTCCTTC TCGAGTTGCA
    TCAACCTTAA CCCGAGACAA TGTTGTTACC GTGTCCCCAT ACTCTCTCAC TCATGTTGCG
    GGACCCTACA ATCGTTGGGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20533
    Clone ID Related Accession (Same CDS sequence) XM_011243509.2 , NM_001253857.2 , NM_001253857.1
    Accession Version NM_001253857.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6120bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Mus musculus(house mouse)
    Product methylcytosine dioxygenase TET1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166359.3. On or before Jun 22, 2017 this sequence version replaced XM_011243509.2, NM_001253857.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: GU079948.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG
    AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC
    CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT
    ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA
    TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT
    GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC
    GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG
    GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC
    GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG
    AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG
    CAGAACCACA ATAAATCAGG TTCACACAAC ACTAAGAGCT TTTCATCAGC CTCATCTACT
    TCTCACCTAG TGAAAGACGA ATCTACAGAC TTCTGTCCCC TGCAGGCTTC CTCCGCAGAA
    ACATCTACCT GTACGTACAG TAAAACAGCC TCAGGTGGGT TTGCAGAAAC AAGTAGTATT
    CTCCACTGCA CAATGCCTTC TGGAGCACAC AGTGGTGCTA ATGCAGCTGC TGGGGAATGT
    ACTGGAACGG TGCAGCCTGC CGAGGTGGCT GCTCATCCTC ACCAGTCTCT TCCCACAGCC
    GATTCTCCCG TTCATGCTGA GCCTCTCACT AGTCCATCTG AGCAGCTAAC TTCTAACCAG
    TCAAACCAGC AGCTCCCTCT CCTCAGCAAT TCTCAGAAAC TGGCTTCCTG TCAGGTGGAA
    GATGAGCGGC ACCCTGAAGC GGATGAGCCT CAGCACCCCG AGGACGATAA CTTGCCTCAA
    CTTGATGAAT TCTGGTCAGA CAGTGAGGAG ATCTACGCCG ATCCTTCCTT TGGTGGCGTG
    GCGATAGCAC CCATTCACGG CTCGGTGCTC ATTGAGTGCG CTCGGAAGGA GCTTCATGCT
    ACCACCTCTT TGCGCTCCCC CAAACGAGGG GTCCCTTTTC GTGTGTCCCT TGTATTCTAC
    CAGCACAAAA GCCTAAACAA GCCTAATCAT GGTTTTGATA TCAACAAAAT TAAGTGTAAA
    TGCAAAAAAG TAACGAAAAA AAAGCCCGCA GACCGGGAGT GTCCTGATGT ATCCCCCGAA
    GCCAATTTAT CACACCAAAT TCCTTCTCGA GTTGCATCAA CCTTAACCCG AGACAATGTT
    GTTACCGTGT CCCCATACTC TCTCACTCAT GTTGCGGGAC CCTACAATCG TTGGGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001240786.1
    CDS273..6392
    Translation

    Target ORF information:

    RefSeq Version NM_001253857.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001253857.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG
    AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC
    CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT
    ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA
    TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT
    GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC
    GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG
    GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC
    GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG
    AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG
    CAGAACCACA ATAAATCAGG TTCACACAAC ACTAAGAGCT TTTCATCAGC CTCATCTACT
    TCTCACCTAG TGAAAGACGA ATCTACAGAC TTCTGTCCCC TGCAGGCTTC CTCCGCAGAA
    ACATCTACCT GTACGTACAG TAAAACAGCC TCAGGTGGGT TTGCAGAAAC AAGTAGTATT
    CTCCACTGCA CAATGCCTTC TGGAGCACAC AGTGGTGCTA ATGCAGCTGC TGGGGAATGT
    ACTGGAACGG TGCAGCCTGC CGAGGTGGCT GCTCATCCTC ACCAGTCTCT TCCCACAGCC
    GATTCTCCCG TTCATGCTGA GCCTCTCACT AGTCCATCTG AGCAGCTAAC TTCTAACCAG
    TCAAACCAGC AGCTCCCTCT CCTCAGCAAT TCTCAGAAAC TGGCTTCCTG TCAGGTGGAA
    GATGAGCGGC ACCCTGAAGC GGATGAGCCT CAGCACCCCG AGGACGATAA CTTGCCTCAA
    CTTGATGAAT TCTGGTCAGA CAGTGAGGAG ATCTACGCCG ATCCTTCCTT TGGTGGCGTG
    GCGATAGCAC CCATTCACGG CTCGGTGCTC ATTGAGTGCG CTCGGAAGGA GCTTCATGCT
    ACCACCTCTT TGCGCTCCCC CAAACGAGGG GTCCCTTTTC GTGTGTCCCT TGTATTCTAC
    CAGCACAAAA GCCTAAACAA GCCTAATCAT GGTTTTGATA TCAACAAAAT TAAGTGTAAA
    TGCAAAAAAG TAACGAAAAA AAAGCCCGCA GACCGGGAGT GTCCTGATGT ATCCCCCGAA
    GCCAATTTAT CACACCAAAT TCCTTCTCGA GTTGCATCAA CCTTAACCCG AGACAATGTT
    GTTACCGTGT CCCCATACTC TCTCACTCAT GTTGCGGGAC CCTACAATCG TTGGGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20533
    Clone ID Related Accession (Same CDS sequence) XM_011243509.2 , NM_001253857.2 , NM_001253857.1
    Accession Version NM_001253857.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6120bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-22
    Organism Mus musculus(House mouse)
    Product methylcytosine dioxygenase TET1 isoform 1
    Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166359.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GU079948.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02146468 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG
    AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC
    CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT
    ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA
    TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT
    GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC
    GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG
    GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC
    GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG
    AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG
    CAGAACCACA ATAAATCAGG TTCACACAAC ACTAAGAGCT TTTCATCAGC CTCATCTACT
    TCTCACCTAG TGAAAGACGA ATCTACAGAC TTCTGTCCCC TGCAGGCTTC CTCCGCAGAA
    ACATCTACCT GTACGTACAG TAAAACAGCC TCAGGTGGGT TTGCAGAAAC AAGTAGTATT
    CTCCACTGCA CAATGCCTTC TGGAGCACAC AGTGGTGCTA ATGCAGCTGC TGGGGAATGT
    ACTGGAACGG TGCAGCCTGC CGAGGTGGCT GCTCATCCTC ACCAGTCTCT TCCCACAGCC
    GATTCTCCCG TTCATGCTGA GCCTCTCACT AGTCCATCTG AGCAGCTAAC TTCTAACCAG
    TCAAACCAGC AGCTCCCTCT CCTCAGCAAT TCTCAGAAAC TGGCTTCCTG TCAGGTGGAA
    GATGAGCGGC ACCCTGAAGC GGATGAGCCT CAGCACCCCG AGGACGATAA CTTGCCTCAA
    CTTGATGAAT TCTGGTCAGA CAGTGAGGAG ATCTACGCCG ATCCTTCCTT TGGTGGCGTG
    GCGATAGCAC CCATTCACGG CTCGGTGCTC ATTGAGTGCG CTCGGAAGGA GCTTCATGCT
    ACCACCTCTT TGCGCTCCCC CAAACGAGGG GTCCCTTTTC GTGTGTCCCT TGTATTCTAC
    CAGCACAAAA GCCTAAACAA GCCTAATCAT GGTTTTGATA TCAACAAAAT TAAGTGTAAA
    TGCAAAAAAG TAACGAAAAA AAAGCCCGCA GACCGGGAGT GTCCTGATGT ATCCCCCGAA
    GCCAATTTAT CACACCAAAT TCCTTCTCGA GTTGCATCAA CCTTAACCCG AGACAATGTT
    GTTACCGTGT CCCCATACTC TCTCACTCAT GTTGCGGGAC CCTACAATCG TTGGGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001240786.1
    CDS1..6120
    Translation

    Target ORF information:

    RefSeq Version NM_001253857.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001253857.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG
    AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC
    CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT
    ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA
    TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT
    GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC
    GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG
    GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC
    GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG
    AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG
    CAGAACCACA ATAAATCAGG TTCACACAAC ACTAAGAGCT TTTCATCAGC CTCATCTACT
    TCTCACCTAG TGAAAGACGA ATCTACAGAC TTCTGTCCCC TGCAGGCTTC CTCCGCAGAA
    ACATCTACCT GTACGTACAG TAAAACAGCC TCAGGTGGGT TTGCAGAAAC AAGTAGTATT
    CTCCACTGCA CAATGCCTTC TGGAGCACAC AGTGGTGCTA ATGCAGCTGC TGGGGAATGT
    ACTGGAACGG TGCAGCCTGC CGAGGTGGCT GCTCATCCTC ACCAGTCTCT TCCCACAGCC
    GATTCTCCCG TTCATGCTGA GCCTCTCACT AGTCCATCTG AGCAGCTAAC TTCTAACCAG
    TCAAACCAGC AGCTCCCTCT CCTCAGCAAT TCTCAGAAAC TGGCTTCCTG TCAGGTGGAA
    GATGAGCGGC ACCCTGAAGC GGATGAGCCT CAGCACCCCG AGGACGATAA CTTGCCTCAA
    CTTGATGAAT TCTGGTCAGA CAGTGAGGAG ATCTACGCCG ATCCTTCCTT TGGTGGCGTG
    GCGATAGCAC CCATTCACGG CTCGGTGCTC ATTGAGTGCG CTCGGAAGGA GCTTCATGCT
    ACCACCTCTT TGCGCTCCCC CAAACGAGGG GTCCCTTTTC GTGTGTCCCT TGTATTCTAC
    CAGCACAAAA GCCTAAACAA GCCTAATCAT GGTTTTGATA TCAACAAAAT TAAGTGTAAA
    TGCAAAAAAG TAACGAAAAA AAAGCCCGCA GACCGGGAGT GTCCTGATGT ATCCCCCGAA
    GCCAATTTAT CACACCAAAT TCCTTCTCGA GTTGCATCAA CCTTAACCCG AGACAATGTT
    GTTACCGTGT CCCCATACTC TCTCACTCAT GTTGCGGGAC CCTACAATCG TTGGGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20533
    Clone ID Related Accession (Same CDS sequence) XM_011243509.2 , NM_001253857.2 , NM_001253857.1
    Accession Version XM_011243509.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6120bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product methylcytosine dioxygenase TET1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243509.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG
    AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC
    CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT
    ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA
    TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT
    GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC
    GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG
    GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC
    GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG
    AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG
    CAGAACCACA ATAAATCAGG TTCACACAAC ACTAAGAGCT TTTCATCAGC CTCATCTACT
    TCTCACCTAG TGAAAGACGA ATCTACAGAC TTCTGTCCCC TGCAGGCTTC CTCCGCAGAA
    ACATCTACCT GTACGTACAG TAAAACAGCC TCAGGTGGGT TTGCAGAAAC AAGTAGTATT
    CTCCACTGCA CAATGCCTTC TGGAGCACAC AGTGGTGCTA ATGCAGCTGC TGGGGAATGT
    ACTGGAACGG TGCAGCCTGC CGAGGTGGCT GCTCATCCTC ACCAGTCTCT TCCCACAGCC
    GATTCTCCCG TTCATGCTGA GCCTCTCACT AGTCCATCTG AGCAGCTAAC TTCTAACCAG
    TCAAACCAGC AGCTCCCTCT CCTCAGCAAT TCTCAGAAAC TGGCTTCCTG TCAGGTGGAA
    GATGAGCGGC ACCCTGAAGC GGATGAGCCT CAGCACCCCG AGGACGATAA CTTGCCTCAA
    CTTGATGAAT TCTGGTCAGA CAGTGAGGAG ATCTACGCCG ATCCTTCCTT TGGTGGCGTG
    GCGATAGCAC CCATTCACGG CTCGGTGCTC ATTGAGTGCG CTCGGAAGGA GCTTCATGCT
    ACCACCTCTT TGCGCTCCCC CAAACGAGGG GTCCCTTTTC GTGTGTCCCT TGTATTCTAC
    CAGCACAAAA GCCTAAACAA GCCTAATCAT GGTTTTGATA TCAACAAAAT TAAGTGTAAA
    TGCAAAAAAG TAACGAAAAA AAAGCCCGCA GACCGGGAGT GTCCTGATGT ATCCCCCGAA
    GCCAATTTAT CACACCAAAT TCCTTCTCGA GTTGCATCAA CCTTAACCCG AGACAATGTT
    GTTACCGTGT CCCCATACTC TCTCACTCAT GTTGCGGGAC CCTACAATCG TTGGGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011241811.1
    CDS518..6637
    Translation

    Target ORF information:

    RefSeq Version XM_011243509.2
    Organism Mus musculus(House mouse)
    Definition Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011243509.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG
    AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC
    CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT
    ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA
    TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT
    GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC
    GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG
    GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC
    GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG
    AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG
    CAGAACCACA ATAAATCAGG TTCACACAAC ACTAAGAGCT TTTCATCAGC CTCATCTACT
    TCTCACCTAG TGAAAGACGA ATCTACAGAC TTCTGTCCCC TGCAGGCTTC CTCCGCAGAA
    ACATCTACCT GTACGTACAG TAAAACAGCC TCAGGTGGGT TTGCAGAAAC AAGTAGTATT
    CTCCACTGCA CAATGCCTTC TGGAGCACAC AGTGGTGCTA ATGCAGCTGC TGGGGAATGT
    ACTGGAACGG TGCAGCCTGC CGAGGTGGCT GCTCATCCTC ACCAGTCTCT TCCCACAGCC
    GATTCTCCCG TTCATGCTGA GCCTCTCACT AGTCCATCTG AGCAGCTAAC TTCTAACCAG
    TCAAACCAGC AGCTCCCTCT CCTCAGCAAT TCTCAGAAAC TGGCTTCCTG TCAGGTGGAA
    GATGAGCGGC ACCCTGAAGC GGATGAGCCT CAGCACCCCG AGGACGATAA CTTGCCTCAA
    CTTGATGAAT TCTGGTCAGA CAGTGAGGAG ATCTACGCCG ATCCTTCCTT TGGTGGCGTG
    GCGATAGCAC CCATTCACGG CTCGGTGCTC ATTGAGTGCG CTCGGAAGGA GCTTCATGCT
    ACCACCTCTT TGCGCTCCCC CAAACGAGGG GTCCCTTTTC GTGTGTCCCT TGTATTCTAC
    CAGCACAAAA GCCTAAACAA GCCTAATCAT GGTTTTGATA TCAACAAAAT TAAGTGTAAA
    TGCAAAAAAG TAACGAAAAA AAAGCCCGCA GACCGGGAGT GTCCTGATGT ATCCCCCGAA
    GCCAATTTAT CACACCAAAT TCCTTCTCGA GTTGCATCAA CCTTAACCCG AGACAATGTT
    GTTACCGTGT CCCCATACTC TCTCACTCAT GTTGCGGGAC CCTACAATCG TTGGGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu44647
    Clone ID Related Accession (Same CDS sequence) XM_006513867.3 , XM_011243508.2 , XM_006513867.4 , XM_006513869.3
    Accession Version XM_006513867.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product methylcytosine dioxygenase TET1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513867.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG
    AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC
    CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT
    ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA
    TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT
    GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC
    GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG
    GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC
    GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG
    AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG
    CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA
    AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC
    CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT
    ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC
    TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA
    ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT
    CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC
    CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG
    CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT
    GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA
    GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC
    TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC
    AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA
    AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT
    TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC
    GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006513930.1
    CDS519..6692
    Translation

    Target ORF information:

    RefSeq Version XM_006513867.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006513867.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG
    AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC
    CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT
    ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA
    TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT
    GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC
    GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG
    GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC
    GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG
    AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG
    CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA
    AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC
    CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT
    ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC
    TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA
    ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT
    CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC
    CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG
    CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT
    GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA
    GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC
    TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC
    AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA
    AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT
    TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC
    GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu44647
    Clone ID Related Accession (Same CDS sequence) XM_006513867.3 , XM_011243508.2 , XM_006513867.4 , XM_006513869.3
    Accession Version XM_011243508.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product methylcytosine dioxygenase TET1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243508.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG
    AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC
    CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT
    ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA
    TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT
    GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC
    GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG
    GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC
    GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG
    AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG
    CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA
    AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC
    CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT
    ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC
    TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA
    ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT
    CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC
    CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG
    CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT
    GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA
    GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC
    TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC
    AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA
    AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT
    TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC
    GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011241810.1
    CDS335..6508
    Translation

    Target ORF information:

    RefSeq Version XM_011243508.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011243508.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG
    AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC
    CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT
    ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA
    TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT
    GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC
    GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG
    GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC
    GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG
    AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG
    CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA
    AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC
    CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT
    ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC
    TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA
    ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT
    CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC
    CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG
    CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT
    GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA
    GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC
    TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC
    AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA
    AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT
    TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC
    GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu44647
    Clone ID Related Accession (Same CDS sequence) XM_006513867.3 , XM_011243508.2 , XM_006513867.4 , XM_006513869.3
    Accession Version XM_006513869.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product methylcytosine dioxygenase TET1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513869.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG
    AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC
    CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT
    ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA
    TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT
    GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC
    GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG
    GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC
    GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG
    AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG
    CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA
    AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC
    CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT
    ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC
    TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA
    ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT
    CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC
    CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG
    CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT
    GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA
    GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC
    TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC
    AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA
    AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT
    TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC
    GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006513932.1
    CDS278..6451
    Translation

    Target ORF information:

    RefSeq Version XM_006513869.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006513869.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCTCGGT CCCGCCCCGC AAAGCCTTCC AAATCAGTCA AAACAAAGCT ACAGAAAAAG 
    AAAGACATCC AGATGAAGAC GAAGACATCA AAGCAGGCTG TCAGACATGG GGCATCAGCG
    AAGGCAGTGA ACCCCGGAAA ACCGAAGCAA TTAATTAAAA GAAGAGATGG TAAGAAGGAA
    ACGGAAGACA AAACCCCGAC ACCGGCGCCG AGTTTTCTGA CGCGAGCCGG GGCCGCCCGA
    ATGAATCGGG ATCGAAACCA AGTTCTTTTT CAGAACCCAG ACTCCTTAAC TTGCAACGGG
    TTTACAATGG CTCTTCGAAG AACCTCTCTT AGCTGGCGAC TCTCCCAGCG CCCAGTAGTC
    ACACCCAAAC CTAAGAAAGT ACCACCTTCA AAGAAGCAGT GTACACATAA TATCCAGGAC
    GAGCCGGGGG TAAAGCACTC TGAAAATGAT TCAGTCCCCA GCCAACACGC TACCGTGTCC
    CCAGGCACAG AGAATGGTGA ACAGAACAGG TGTTTAGTTG AAGGGGAGAG CCAAGAGATA
    ACCCAGTCTT GCCCTGTATT TGAGGAAAGA ATAGAAGATA CCCAAAGCTG TATTTCCGCA
    TCTGGGAACC TGGAAGCAGA AATTTCTTGG CCACTGGAAG GGACACATTG TGAAGAACTA
    CTTTCTCATC AGACATCTGA TAATGAGTGC ACTTCCCCTC AAGAGTGTGC TCCATTGCCC
    CAAAGATCAA CTTCCGAAGT TACCTCTCAG AAAAACACTA GCAATCAGTT AGCAGACTTG
    AGCTCACAAG TAGAGTCTAT CAAGCTGTCT GATCCTTCTC CGAATCCTAC GGGAAGCGAC
    CATAATGGCT TTCCAGACTC CAGTTTTAGG ATCGTTCCTG AATTGGACCT GAAAACCTGC
    ATGCCTCTTG ATGAGTCTGT GTATCCCACA GCTCTGATAA GATTCATCTT GGCAGGCTCA
    CAGCCGGATG TCTTTGATAC TAAGCCCCAA GAAAAAACCC TTATAACAAC CCCAGAACAA
    GTGGGAAGCC ACCCAAATCA AGTTCTGGAT GCCACCTCTG TCCTAGGACA GGCCTTTAGT
    ACTCTTCCAC TTCAGTGGGG ATTTTCTGGT GCTAACTTAG TTCAAGTTGA GGCCCTGGGC
    AAGGGTTCAG ACTCACCAGA GGATCTTGGT GCTATTACTA TGCTAAACCA ACAAGAAACT
    GTGGCTATGG ATATGGATAG GAATGCTACC CCGGACCTGC CTATCTTCCT TCCTAAGCCT
    CCAAACACAG TTGCTACCTA CAGTAGCCCT CTTTTAGGGC CTGAGCCCCA CAGCTCTACC
    TCCTGTGGGC TTGAAGTCCA AGGTGCTACC CCGATTTTAA CTTTGGACTC AGGACACACT
    CCCCAACTCC CACCAAACCC TGAGTCAAGT TCAGTACCTC TGGTAATAGC TGCAAACGGT
    ACGCGAGCTG AGAAGCAGTT TGGTACCAGC CTTTTCCCAG CCGTCCCACA AGGGTTTACA
    GTCGCAGCCG AGAATGAAGT CCAGCATGCC CCATTAGATC TTACCCAGGG TTCTCAGGCT
    GCTCCCAGCA AACTGGAAGG AGAGATTTCT CGGGTCAGCA TCACTGGCTC AGCTGATGTC
    AAAGCCACAG CGATGTCCAT GCCGGTTACA CAGGCCAGTA CCTCTTCTCC CCCATGTAAT
    TCTACGCCGC CAATGGTGGA GAGGAGGAAG CGCAAGGCCT GTGGGGTCTG TGAGCCGTGC
    CAGCAGAAGG CCAACTGTGG GGAGTGCACC TACTGCAAGA ATCGAAAGAA CAGCCACCAG
    ATCTGTAAGA AGAGGAAATG CGAGGTGCTC AAAAAGAAGC CAGAGGCCAC CTCACAGGCA
    CAGGTTACAA AAGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGACA
    GACTTTAACA ACAAACCAGT AAATGGCCCC AAATCAGAGT CCATGGACTG CAGTAGACGT
    GGTCATGGGG AAGAAGAGCA AAGGTTGGAT TTGATCACAC ATCCCCTTGA AAATGTAAGG
    AAGAATGCAG GCGGCATGAC GGGAATTGAG GTGGAGAAGT GGGCGCCAAA CAAGAAATCA
    CACTTAGCAG AGGGTCAAGT CAAAGGAAGC TGTGATGCCA ATTTAACTGG GGTGGAAAAC
    CCCCAGCCCT CTGAAGATGA CAAGCAGCAA ACCAACCCTT CTCCGACATT TGCCCAGACC
    ATAAGGAACG GCATGAAAAA TGTACACTGT TTACCCACTG ACACACACCT TCCGCTCAAC
    AAATTGAATC ATGAGGAATT TAGCAAGGCG TTGGGAAATA ATTCCTCTAA ATTGCTGACT
    GATCCCTCAA ACTGCAAGGA TGCCATGAGT GTCACCACCT CCGGGGGGGA GTGTGATCAT
    CTCAAGGGGC CAAGGAACAC CCTGCTCTTC CAGAAGCCTG GCTTGAACTG CAGGTCTGGT
    GCAGAACCCA CCATCTTTAA TAATCACCCT AATACACACA GCGCTGGGAG TCGACCTCAC
    CCTCCTGAGA AGGTACCCAA CAAAGAACCC AAAGATGGCT CTCCGGTTCA GCCAAGTCTT
    CTATCCCTAA TGAAGGACAG GAGATTAACG CTGGAACAAG TGGTAGCCAT AGAAGCCCTG
    ACTCAGCTCT CAGAAGCGCC CTCAGAGAGC TCCTCCCCAT CCAAGCCGGA GAAGGATGAA
    GAAGCGCATC AGAAAACGGC TAGTCTGCTT AACAGTTGCA AGGCTATCCT TCATTCGGTG
    AGAAAAGACC TCCAAGACCC AAACGTTCAA GGAAAGGGCC TTCACCATGA CACTGTTGTC
    TTTAATGGCC AGAACAGAAC ATTCAAGTCA CCTGATAGTT TCGCTACTAA CCAAGCACTT
    ATAAAATCAC AAGGATATCC AAGTTCACCT ACAGCTGAGA AGAAAGGCGC TGCTGGTGGG
    AGAGCTCCCT TTGATGGTTT TGAGAATTCA CATCCATTGC CCATAGAAAG TCACAATCTT
    GAAAACTGCA GTCAGGTACT AAGCTGCGAT CAGAATTTGA GTTCCCATGA CCCATCATGC
    CAGGATGCAC CCTACAGTCA AATTGAAGAA GATGTCGCAG CACAATTGAC CCAACTTGCA
    TCCACGATTA ATCACATCAA CGCCGAGGTC AGGAATGCTG AAAGTACACC AGAAAGCCTT
    GTTGCAAAGA ACACAAAGCA AAAACACAGT CAGGAGAAAC GCATGGTACA TCAGAAGCCA
    CCCTCTAGTA CACAAACTAA ACCCAGTGTA CCATCAGCAA AGCCAAAGAA GGCCCAGAAA
    AAGGCAAGAG CGACCCCACA CGCAAACAAG CGGAAAAAGA AACCCCCAGC CCGAAGCTCT
    CAAGAAAATG ATCAGAAGAA GCAAGAGCAG CTAGCTATAG AGTATAGTAA AATGCATGAC
    ATTTGGATGT CATCTAAGTT TCAAAGGTTT GGCCAGTCTA GTCCACGTAG TTTTCCTGTT
    CTGTTGAGAA ATATTCCTGT CTTTAACCAA ATATTAAAGC CCGTGACTCA GAGTAAAACA
    CCCTCACAAC ATAATGAGTT ATTTCCTCCT ATCAATCAGA TAAAATTCAC AAGAAACCCT
    GAATTGGCAA AGGAGAAAGT GAAGGTTGAA CCATCAGATT CTCTGCCAAC ATGCCAATTC
    AAGACAGAAT CCGGTGGGCA GACATTTGCA GAGCCGGCAG ATAATTCTCA GGGACAGCCC
    ATGGTGAGTG TCAATCAGGA AGCTCACCCT CTGCCCCAGT CCCCACCATC TAACCAGTGT
    GCTAATATAA TGGCTGGTGC TGCCCAAACA CAGTTTCATC TAGGCGCTCA AGAGAACCTG
    GTGCATCAGA TACCACCACC GACGTTGCCT GGTACCTCCC CTGACACACT CTTGCCAGAC
    CCAGCATCAA TCCTCAGGAA AGGGAAAGTG TTACATTTCG ATGGAATTAC AGTTGTTACG
    GAGAAGCGTG AAGCTCAAAC ATCAAGCAAT GGACCACTGG GTCCCACCAC AGACAGTGCA
    CAGTCAGAAT TTAAAGAGAG CATCATGGAC TTACTTAGCA AGCCTGCAAA AAACCTGATA
    GCTGGATTGA AGGAACAGGA AGCTGCACCC TGTGACTGTG ATGGAGGTAC ACAAAAAGAA
    AAAGGCCCAT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC
    ATGGAGACTA GGTTTGGCCA GAAGGGGAAG GCAATCCGGA TTGAGAAGAT AGTGTTCACG
    GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT CAGAAGAAGT
    GGTCCTGAAG AGAAGCTTAT TTGTTTGGTT CGTGAGCGTG TAGACCATCA CTGTTCGACG
    GCTGTGATAG TTGTCCTTAT CCTGCTGTGG GAAGGTATCC CTCGCCTGAT GGCTGACCGC
    CTGTACAAAG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC AGACCGAAGA
    TGTACCCTCA ACAAAAAGCG TACCTGCACC TGTCAAGGCA TCGACCCAAA AACCTGCGGA
    GCGTCCTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGG
    AGTGAAAACC CCAGAAAATT CAGACTTGCT CCAAACTACC CCTTACATAA CTACTATAAG
    AGAATTACTG GAATGAGTTC TGAAGGAAGT GACGTGAAAA CCGGGTGGAT CATTCCAGAC
    CGCAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAAA AGAATTTACA AGAATTGGCT
    ACAGTATTAG CTCCACTTTA CAAGCAGATG GCTCCAGTTG CTTATCAAAA TCAGGTGGAA
    TATGAAGAAG TTGCTGGAGA CTGTCGACTT GGAAATGAAG AGGGGCGTCC TTTCTCTGGT
    GTCACCTGTT GCATGGATTT TTGTGCCCAT TCTCACAAGG ACATTCACAA CATGCACAAC
    GGAAGCACCG TGGTGTGTAC GTTGATTCGA GCAGATGGCC GTGACACAAA TTGTCCCGAG
    GATGAACAAC TCCACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC
    GTGGAAGGGA TGAAGGCCAA AATCAAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG
    AAGAGGCGAC TACGTTTTAC TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAAATGAAG
    CAGAACCACA ATAAATCAGG TACTGCTGGT CTCAGAAGGA AGAGGATCTC TGCCAGCCCA
    AAGGGAGCCC CAGGTTCACA CAACACTAAG AGCTTTTCAT CAGCCTCATC TACTTCTCAC
    CTAGTGAAAG ACGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCGC AGAAACATCT
    ACCTGTACGT ACAGTAAAAC AGCCTCAGGT GGGTTTGCAG AAACAAGTAG TATTCTCCAC
    TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTGCTGGGGA ATGTACTGGA
    ACGGTGCAGC CTGCCGAGGT GGCTGCTCAT CCTCACCAGT CTCTTCCCAC AGCCGATTCT
    CCCGTTCATG CTGAGCCTCT CACTAGTCCA TCTGAGCAGC TAACTTCTAA CCAGTCAAAC
    CAGCAGCTCC CTCTCCTCAG CAATTCTCAG AAACTGGCTT CCTGTCAGGT GGAAGATGAG
    CGGCACCCTG AAGCGGATGA GCCTCAGCAC CCCGAGGACG ATAACTTGCC TCAACTTGAT
    GAATTCTGGT CAGACAGTGA GGAGATCTAC GCCGATCCTT CCTTTGGTGG CGTGGCGATA
    GCACCCATTC ACGGCTCGGT GCTCATTGAG TGCGCTCGGA AGGAGCTTCA TGCTACCACC
    TCTTTGCGCT CCCCCAAACG AGGGGTCCCT TTTCGTGTGT CCCTTGTATT CTACCAGCAC
    AAAAGCCTAA ACAAGCCTAA TCATGGTTTT GATATCAACA AAATTAAGTG TAAATGCAAA
    AAAGTAACGA AAAAAAAGCC CGCAGACCGG GAGTGTCCTG ATGTATCCCC CGAAGCCAAT
    TTATCACACC AAATTCCTTC TCGAGTTGCA TCAACCTTAA CCCGAGACAA TGTTGTTACC
    GTGTCCCCAT ACTCTCTCAC TCATGTTGCG GGACCCTACA ATCGTTGGGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.