Mfhas1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mfhas1
Entrez Gene ID	52065
Full Name	malignant fibrous histiocytoma amplified sequence 1
Synonyms	2310066G09Rik,D8Ertd91e
General protein information	Preferred Names malignant fibrous histiocytoma amplified sequence 1 Names malignant fibrous histiocytoma-amplified sequence 1 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 A4\|8 21.16 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006509170.2	XP_006509233.1	malignant fibrous histiocytoma-amplified sequence 1 homolog isoform X1
NM_001081279.1	NP_001074748.1	malignant fibrous histiocytoma-amplified sequence 1 homolog
NM_001081279.2	NP_001074748.1	malignant fibrous histiocytoma-amplified sequence 1 homolog isoform 1
NM_001364259.1	NP_001351188.1	malignant fibrous histiocytoma-amplified sequence 1 homolog isoform 2

Pathways from BioSystems

Homologies

	MFHAS1	XP_001090936.1
Bos taurus (cattle)	MFHAS1	XP_003588007.1
Xenopus tropicalis (tropical clawed frog)	mfhas1	NP_001096407.1
Rattus norvegicus (Norway rat)	Mfhas1	NP_001100786.1
Gallus gallus (chicken)	LOC101750338	XP_004941212.1
Homo sapiens (human)	MFHAS1	NP_004216.2
Pan troglodytes (chimpanzee)	MFHAS1	XP_528066.2
Mus musculus (house mouse)	Mfhas1	NP_001074748.1
Danio rerio (zebrafish)	mfhas1	XP_001335202.2
Canis lupus familiaris (dog)	MFHAS1	XP_532819.3

Gene Ontology
Bibliography

Related articles in PubMed

MFHAS1 promotes colorectal cancer progress by regulating polarization of tumor-associated macrophages via STAT6 signaling pathway.
Chen W, Xu Y, Zhong J, Wang H, Weng M, Cheng Q, Wu Q, Sun Z, Jiang H, Zhu M, Ren Y, Xu P, Chen J, Miao C
Oncotarget7(48)78726-78735(2016 Nov)

MFHAS1 Is Associated with Sepsis and Stimulates TLR2/NF-?B Signaling Pathway Following Negative Regulation.
Zhong J, Shi QQ, Zhu MM, Shen J, Wang HH, Ma D, Miao CH
PloS one10(11)e0143662(2015)

Human leucine-rich repeat proteins: a genome-wide bioinformatic categorization and functional analysis in innate immunity.
Ng AC, Eisenberg JM, Heath RJ, Huett A, Robinson CM, Nau GJ, Xavier RJ
Proceedings of the National Academy of Sciences of the United States of America108 Suppl 14631-8(2011 Mar)

A high-resolution anatomical atlas of the transcriptome in the mouse embryo.
Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, Magen A, Canidio E, Pagani M, Peluso I, Lin-Marq N, Koch M, Bilio M, Cantiello I, Verde R, De Masi C, Bianchi SA, Cicchini J, Perroud E, Mehmeti S, Dagand E, Schrinner S, N?rnberger A, Schmidt K, Metz K, Zwingmann C, Brieske N, Springer C, Hernandez AM, Herzog S, Grabbe F, Sieverding C, Fischer B, Schrader K, Brockmeyer M, Dettmer S, Helbig C, Alunni V, Battaini MA, Mura C, Henrichsen CN, Garcia-Lopez R, Echevarria D, Puelles E, Garcia-Calero E, Kruse S, Uhr M, Kauck C, Feng G, Milyaev N, Ong CK, Kumar L, Lam M, Semple CA, Gyenesei A, Mundlos S, Radelof U, Lehrach H, Sarmientos P, Reymond A, Davidson DR, Doll? P, Antonarakis SE, Yaspo ML, Martinez S, Baldock RA, Eichele G, Ballabio A
PLoS biology9(1)e1000582(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mfhas1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mfhas1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69423	NM_001364259.1 Latest version!	Mus musculus malignant fibrous histiocytoma amplified sequence 1 (Mfhas1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu02019	NM_001081279.2 Latest version!	Mus musculus malignant fibrous histiocytoma amplified sequence 1 (Mfhas1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$615.30 $879.00
OMu40676	XM_006509170.2 Latest version!	Mus musculus malignant fibrous histiocytoma amplified sequence 1 (Mfhas1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu02019	NM_001081279.1	Mus musculus malignant fibrous histiocytoma amplified sequence 1 (Mfhas1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$615.30 $879.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mfhas1 ( NM_001364259.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001351188.1 Mus musculus malignant fibrous histiocytoma amplified sequence 1 (Mfhas1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69423
Clone ID Related Accession (Same CDS sequence)	NM_001364259.1
Accession Version	NM_001364259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Mus musculus(house mouse)
Product	malignant fibrous histiocytoma-amplified sequence 1 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC129082.6 and AC129211.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK171759.1, BY571371.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGCC AGGACAGTGG CAACCTGAAG ACGGTGAGGC TGTGGCGGGA CGCCGCCCTG 
AGAGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TCAGCTGCCC AGGGGCCGGA 
GGCGACCCAC TGGAGTCCCC CGACGCCCCC CAGCTGGTGC TGCCGGCCAA CATCGGGGAC 
ATTGAGGTGC TGAACCTGGG GAACAACGGC CTCGAGGATG TGCCTGAAGG TCTGGGGTCA 
GCTCTGGGCA GCCTTCGCGT CTTGGTCTTG CGCAGGAACC GTTTTGCCCG GCTGCCCCCT 
GCGGTGGCGG AGCTAGGCCA CCACCTTACC GAACTGGACG TCAGCCACAA CCGGTTGACC 
ATCCTGGGAG CCGAGGTGGT GAGCGCCCTG AGGGAGCTGC GCAAACTCAA CCTGAGCCAC 
AACCAGTTGC CAGCCCTGCC TGCCCAGCTG GGTGCCCTCG CCCACCTGGA GGAGCTGGAT 
GTCAGCTTCA ACCGGCTGGC GCATCTGCCA GATTCCTTCT CCTGCCTCAA CCACCTTCGG 
ACCCTTGACG TGGACCACAA CCAGCTCACC GCCTTTCCCC AGCAGCTGCT GCAGCTGGCT 
GCCCTGGAGG AGCTCGACGT CTCCAGCAAC CGGCTGCGAG GCCTACCTGA GGATATCAGT 
GCCCTGCGTG CCCTCAAGAT CCTCTGGCTT AGCGGGGCCG AGCTTGGCAC GCTGCCCAGA 
GGCTTCTGCG AGCTGGCCAG TTTGGAGAGC CTCATGTTAG ATAACAACGG GCTCCAGGCT 
CTGCCCGACG AGTTCAGCCG TCTGCAGAGG CTCAAAATGC TCAACCTCTC TTCCAACCTC 
TTCGAGGAGT TCCCTGCTGC GCTGCTGCCC CTGGCTGGTC TGGAGGAGCT CTATCTTAGT 
CGCAATCAGC TCACCTCAGT GCCCTCTCTT ATCGCCGGGC TGGGTCGGCT TCTCACCTTG 
TGGCTGGATA ATAACCGCAT CCGGTACCTG CCAGACTCCA TTGTGGAGCT GACTGGCCTG 
GAGGAGCTGG TGCTCCAGGG CAACCAGATC GCTGTGCTGC CGGACAACTT TGGCCAGCTC 
TCCCGGGTAG GCTTGTGGAA AATCAAGGAC AACCCACTGA TTCAGCCCCC TTACGAAGTC 
TGCATGAAGG GGATCCCTTA CATCGCAGCC TACCAGAAGG AACTGGCGCA TTCGCAGCCA 
GCCGTGCAGC CCCGGCTCAA GCTGCTCCTG ATGGGCCACA AGGCTGCAGG AAAGACCCTG 
CTTCGACATT GCCTCACCGA GGACAAAGTG GAGGGAGGGC AAGGTGGAGG GGACAAGGAG 
AAGAGCTACC TACCTTTCCC TCCCCTTGGT AGTAAGGGCA TCGAGGTGAC CAGCTGGACA 
GCGGATGCTT CCCGGGGCTT GCGGTTCATT GTGTATGACT TAGCCGGTGA TGAAAGCTAT 
GAGGTGATAC AGCCCTTCTT CCTCTCCCCT GGAGCCCTGT ATGTGCTCGT GGTCAACTTG 
GCCACCTATG AACCTCGCTG CTTCCCTACC ACCGTGGGCT CCTTCTTGCA TCGGGTGGGG 
GCCCGGGTGC CTCACGCTGT GGTGTGTATT GTAGGCACGC ATGCGGACCT GTGTGGGGAG 
CGGGAGCTTG AGGAGAAATG TCTGGACATT CACCGACAGA TTGCCCTGCA GGAGAAGAAC 
GATGCAGAGG GCCTTAGCCA TCTAGCCAAG GTGGTGGATG AGGCCCTGGC TCGGGACTTC 
GAGCTGCGCT CTGCCAGCCC TCACGCAGCC TACTACGGTG TTTCAGACAA GAACCTTCGG 
AGGCGCAAGG CCCATTTTCA ATACTTGCTT AACCACCGGT TGCAGATCCT CTCTCCCGTG 
TTGCCCGTAA GCTGCCGGGA CCCCCTCCAA TTGCAACGCC TTCGAGACAA GCTGCTCTCT 
GTAGCTGAGC ACCGAGAAAT CTTTCCCAAC TTACACCGAG TCCTGCCTCG ATCCTGGCAG 
GTACTGGAGG AACTGCACTT CCAGCCACCT CAGGCACAAC GACTGTGGCT GAGCTGGTGG 
GACTCTGCCC GCCTGGGCCT GCAGGCAGGT CTGACTGAGG ACCGGCTCCA GAGTGCTCTT 
TCCTACCTGC ACGAGAGCGG CAAACTGCTC TATTTTGAGG ATAGCCCAGC CCTCAAGGAG 
CATGTCTTCC ACAACCTCAC CCGCCTCATT GACATCCTCA ATGTCTTTTT CCAGAGAGAT 
GCTTCATTGC TGCTGCATAA ACTGCTCTTA GGGACCAATG GAGAGGGGGA GGGGGAGGGG 
GAAAGTTTCC CCACTATAGC AGTGCCCAGC CCGGGTCAGG ACCCACTCCG GGCCACCCAG 
CTTCATCATT ATGTGGAAGG CTTTTTGCTG CATGGGCTTC TGCCAGCCCA CATCATTCGG 
TTGCTGCTTA AGCCTCATGT CCAGGCCCAA CAGGACTTGC AGCTCCTGCT GGAGCTGTTG 
GAGAAGATGG GACTCTGTTA CTGCCTCAAT AAACCCAAAG GCAAGCCTTT GAATGGCTCC 
GCGGCATGGT ACAAGTTCCC ATGCTACGTG CAGAATGAGG TGCCCCATGC AGAGGCCTGG 
ATTAATGGCA CCAACCTAGC CGGGCAGTCT TTTGTGGCTG AACAGTTACA GATCGAATAT 
AGTTTTCCCT TCACCTTTCC ACCTGGATTG TTCGCACGTT ACAGTGTCCA GATCAACAGT 
CACGTGGTGC ACAGGGGAGT TGCTGAGCCA GCCCAGGCCA GAAGGGGTGG CAGAGATCAT 
CTGCCCCAAG AATGGCAGCG AGCGAGTGAA TGTGGCTTTG GTTTACCCAC CTACACCGAC 
TGTGATCAGC CCTTGTTCCA AGAAGAACGT TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351188.1
CDS	585..3440
Translation	MAGQDSGNLKTVRLWRDAALRARKLRSNLRQLTLSCPGAGGDPLESPDAPQLVLPANIGDIEVLNLGNNGLEDVPEGLGSALGSLRVLVLRRNRFARLPPAVAELGHHLTELDVSHNRLTILGAEVVSALRELRKLNLSHNQLPALPAQLGALAHLEELDVSFNRLAHLPDSFSCLNHLRTLDVDHNQLTAFPQQLLQLAALEELDVSSNRLRGLPEDISALRALKILWLSGAELGTLPRGFCELASLESLMLDNNGLQALPDEFSRLQRLKMLNLSSNLFEEFPAALLPLAGLEELYLSRNQLTSVPSLIAGLGRLLTLWLDNNRIRYLPDSIVELTGLEELVLQGNQIAVLPDNFGQLSRVGLWKIKDNPLIQPPYEVCMKGIPYIAAYQKELAHSQPAVQPRLKLLLMGHKAAGKTLLRHCLTEDKVEGGQGGGDKEKSYLPFPPLGSKGIEVTSWTADASRGLRFIVYDLAGDESYEVIQPFFLSPGALYVLVVNLATYEPRCFPTTVGSFLHRVGARVPHAVVCIVGTHADLCGERELEEKCLDIHRQIALQEKNDAEGLSHLAKVVDEALARDFELRSASPHAAYYGVSDKNLRRRKAHFQYLLNHRLQILSPVLPVSCRDPLQLQRLRDKLLSVAEHREIFPNLHRVLPRSWQVLEELHFQPPQAQRLWLSWWDSARLGLQAGLTEDRLQSALSYLHESGKLLYFEDSPALKEHVFHNLTRLIDILNVFFQRDASLLLHKLLLGTNGEGEGEGESFPTIAVPSPGQDPLRATQLHHYVEGFLLHGLLPAHIIRLLLKPHVQAQQDLQLLLELLEKMGLCYCLNKPKGKPLNGSAAWYKFPCYVQNEVPHAEAWINGTNLAGQSFVAEQLQIEYSFPFTFPPGLFARYSVQINSHVVHRGVAEPAQARRGGRDHLPQEWQRASECGFGLPTYTDCDQPLFQEERW

Target ORF information:

RefSeq Version	NM_001364259.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus malignant fibrous histiocytoma amplified sequence 1 (Mfhas1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364259.1

ORF Insert Sequence:

ATGGCTGGCC AGGACAGTGG CAACCTGAAG ACGGTGAGGC TGTGGCGGGA CGCCGCCCTG 
AGAGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TCAGCTGCCC AGGGGCCGGA 
GGCGACCCAC TGGAGTCCCC CGACGCCCCC CAGCTGGTGC TGCCGGCCAA CATCGGGGAC 
ATTGAGGTGC TGAACCTGGG GAACAACGGC CTCGAGGATG TGCCTGAAGG TCTGGGGTCA 
GCTCTGGGCA GCCTTCGCGT CTTGGTCTTG CGCAGGAACC GTTTTGCCCG GCTGCCCCCT 
GCGGTGGCGG AGCTAGGCCA CCACCTTACC GAACTGGACG TCAGCCACAA CCGGTTGACC 
ATCCTGGGAG CCGAGGTGGT GAGCGCCCTG AGGGAGCTGC GCAAACTCAA CCTGAGCCAC 
AACCAGTTGC CAGCCCTGCC TGCCCAGCTG GGTGCCCTCG CCCACCTGGA GGAGCTGGAT 
GTCAGCTTCA ACCGGCTGGC GCATCTGCCA GATTCCTTCT CCTGCCTCAA CCACCTTCGG 
ACCCTTGACG TGGACCACAA CCAGCTCACC GCCTTTCCCC AGCAGCTGCT GCAGCTGGCT 
GCCCTGGAGG AGCTCGACGT CTCCAGCAAC CGGCTGCGAG GCCTACCTGA GGATATCAGT 
GCCCTGCGTG CCCTCAAGAT CCTCTGGCTT AGCGGGGCCG AGCTTGGCAC GCTGCCCAGA 
GGCTTCTGCG AGCTGGCCAG TTTGGAGAGC CTCATGTTAG ATAACAACGG GCTCCAGGCT 
CTGCCCGACG AGTTCAGCCG TCTGCAGAGG CTCAAAATGC TCAACCTCTC TTCCAACCTC 
TTCGAGGAGT TCCCTGCTGC GCTGCTGCCC CTGGCTGGTC TGGAGGAGCT CTATCTTAGT 
CGCAATCAGC TCACCTCAGT GCCCTCTCTT ATCGCCGGGC TGGGTCGGCT TCTCACCTTG 
TGGCTGGATA ATAACCGCAT CCGGTACCTG CCAGACTCCA TTGTGGAGCT GACTGGCCTG 
GAGGAGCTGG TGCTCCAGGG CAACCAGATC GCTGTGCTGC CGGACAACTT TGGCCAGCTC 
TCCCGGGTAG GCTTGTGGAA AATCAAGGAC AACCCACTGA TTCAGCCCCC TTACGAAGTC 
TGCATGAAGG GGATCCCTTA CATCGCAGCC TACCAGAAGG AACTGGCGCA TTCGCAGCCA 
GCCGTGCAGC CCCGGCTCAA GCTGCTCCTG ATGGGCCACA AGGCTGCAGG AAAGACCCTG 
CTTCGACATT GCCTCACCGA GGACAAAGTG GAGGGAGGGC AAGGTGGAGG GGACAAGGAG 
AAGAGCTACC TACCTTTCCC TCCCCTTGGT AGTAAGGGCA TCGAGGTGAC CAGCTGGACA 
GCGGATGCTT CCCGGGGCTT GCGGTTCATT GTGTATGACT TAGCCGGTGA TGAAAGCTAT 
GAGGTGATAC AGCCCTTCTT CCTCTCCCCT GGAGCCCTGT ATGTGCTCGT GGTCAACTTG 
GCCACCTATG AACCTCGCTG CTTCCCTACC ACCGTGGGCT CCTTCTTGCA TCGGGTGGGG 
GCCCGGGTGC CTCACGCTGT GGTGTGTATT GTAGGCACGC ATGCGGACCT GTGTGGGGAG 
CGGGAGCTTG AGGAGAAATG TCTGGACATT CACCGACAGA TTGCCCTGCA GGAGAAGAAC 
GATGCAGAGG GCCTTAGCCA TCTAGCCAAG GTGGTGGATG AGGCCCTGGC TCGGGACTTC 
GAGCTGCGCT CTGCCAGCCC TCACGCAGCC TACTACGGTG TTTCAGACAA GAACCTTCGG 
AGGCGCAAGG CCCATTTTCA ATACTTGCTT AACCACCGGT TGCAGATCCT CTCTCCCGTG 
TTGCCCGTAA GCTGCCGGGA CCCCCTCCAA TTGCAACGCC TTCGAGACAA GCTGCTCTCT 
GTAGCTGAGC ACCGAGAAAT CTTTCCCAAC TTACACCGAG TCCTGCCTCG ATCCTGGCAG 
GTACTGGAGG AACTGCACTT CCAGCCACCT CAGGCACAAC GACTGTGGCT GAGCTGGTGG 
GACTCTGCCC GCCTGGGCCT GCAGGCAGGT CTGACTGAGG ACCGGCTCCA GAGTGCTCTT 
TCCTACCTGC ACGAGAGCGG CAAACTGCTC TATTTTGAGG ATAGCCCAGC CCTCAAGGAG 
CATGTCTTCC ACAACCTCAC CCGCCTCATT GACATCCTCA ATGTCTTTTT CCAGAGAGAT 
GCTTCATTGC TGCTGCATAA ACTGCTCTTA GGGACCAATG GAGAGGGGGA GGGGGAGGGG 
GAAAGTTTCC CCACTATAGC AGTGCCCAGC CCGGGTCAGG ACCCACTCCG GGCCACCCAG 
CTTCATCATT ATGTGGAAGG CTTTTTGCTG CATGGGCTTC TGCCAGCCCA CATCATTCGG 
TTGCTGCTTA AGCCTCATGT CCAGGCCCAA CAGGACTTGC AGCTCCTGCT GGAGCTGTTG 
GAGAAGATGG GACTCTGTTA CTGCCTCAAT AAACCCAAAG GCAAGCCTTT GAATGGCTCC 
GCGGCATGGT ACAAGTTCCC ATGCTACGTG CAGAATGAGG TGCCCCATGC AGAGGCCTGG 
ATTAATGGCA CCAACCTAGC CGGGCAGTCT TTTGTGGCTG AACAGTTACA GATCGAATAT 
AGTTTTCCCT TCACCTTTCC ACCTGGATTG TTCGCACGTT ACAGTGTCCA GATCAACAGT 
CACGTGGTGC ACAGGGGAGT TGCTGAGCCA GCCCAGGCCA GAAGGGGTGG CAGAGATCAT 
CTGCCCCAAG AATGGCAGCG AGCGAGTGAA TGTGGCTTTG GTTTACCCAC CTACACCGAC 
TGTGATCAGC CCTTGTTCCA AGAAGAACGT TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfhas1 ( NM_001081279.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074748.1 Mus musculus malignant fibrous histiocytoma amplified sequence 1 (Mfhas1), mRNA.

Price & Availability↑

CloneID	OMu02019
Clone ID Related Accession (Same CDS sequence)	NM_001081279.1 , NM_001081279.2
Accession Version	NM_001081279.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Mus musculus(house mouse)
Product	malignant fibrous histiocytoma-amplified sequence 1 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC129082.6 and AC129211.3. On Feb 6, 2007 this sequence version replaced XM_884423.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC082308.1, AK171731.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGGCC AGGACAGTGG CAACCTGAAG ACGGTGAGGC TGTGGCGGGA CGCCGCCCTG 
AGAGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TCAGCTGCCC AGGGGCCGGA 
GGCGACCCAC TGGAGTCCCC CGACGCCCCC CAGCTGGTGC TGCCGGCCAA CATCGGGGAC 
ATTGAGGTGC TGAACCTGGG GAACAACGGC CTCGAGGATG TGCCTGAAGG TCTGGGGTCA 
GCTCTGGGCA GCCTTCGCGT CTTGGTCTTG CGCAGGAACC GTTTTGCCCG GCTGCCCCCT 
GCGGTGGCGG AGCTAGGCCA CCACCTTACC GAACTGGACG TCAGCCACAA CCGGTTGACC 
ATCCTGGGAG CCGAGGTGGT GAGCGCCCTG AGGGAGCTGC GCAAACTCAA CCTGAGCCAC 
AACCAGTTGC CAGCCCTGCC TGCCCAGCTG GGTGCCCTCG CCCACCTGGA GGAGCTGGAT 
GTCAGCTTCA ACCGGCTGGC GCATCTGCCA GATTCCTTCT CCTGCCTCAA CCACCTTCGG 
ACCCTTGACG TGGACCACAA CCAGCTCACC GCCTTTCCCC AGCAGCTGCT GCAGCTGGCT 
GCCCTGGAGG AGCTCGACGT CTCCAGCAAC CGGCTGCGAG GCCTACCTGA GGATATCAGT 
GCCCTGCGTG CCCTCAAGAT CCTCTGGCTT AGCGGGGCCG AGCTTGGCAC GCTGCCCAGA 
GGCTTCTGCG AGCTGGCCAG TTTGGAGAGC CTCATGTTAG ATAACAACGG GCTCCAGGCT 
CTGCCCGACG AGTTCAGCCG TCTGCAGAGG CTCAAAATGC TCAACCTCTC TTCCAACCTC 
TTCGAGGAGT TCCCTGCTGC GCTGCTGCCC CTGGCTGGTC TGGAGGAGCT CTATCTTAGT 
CGCAATCAGC TCACCTCAGT GCCCTCTCTT ATCGCCGGGC TGGGTCGGCT TCTCACCTTG 
TGGCTGGATA ATAACCGCAT CCGGTACCTG CCAGACTCCA TTGTGGAGCT GACTGGCCTG 
GAGGAGCTGG TGCTCCAGGG CAACCAGATC GCTGTGCTGC CGGACAACTT TGGCCAGCTC 
TCCCGGGTAG GCTTGTGGAA AATCAAGGAC AACCCACTGA TTCAGCCCCC TTACGAAGTC 
TGCATGAAGG GGATCCCTTA CATCGCAGCC TACCAGAAGG AACTGGCGCA TTCGCAGCCA 
GCCGTGCAGC CCCGGCTCAA GCTGCTCCTG ATGGGCCACA AGGCTGCAGG AAAGACCCTG 
CTTCGACATT GCCTCACCGA GGACAAAGTG GAGGGAGGGC AAGGTGGAGG GGACAAGGAG 
AAGAGCTACC TACCTTTCCC TCCCCTTGGT AGTAAGGGCA TCGAGGTGAC CAGCTGGACA 
GCGGATGCTT CCCGGGGCTT GCGGTTCATT GTGTATGACT TAGCCGGTGA TGAAAGCTAT 
GAGGTGATAC AGCCCTTCTT CCTCTCCCCT GGAGCCCTGT ATGTGCTCGT GGTCAACTTG 
GCCACCTATG AACCTCGCTG CTTCCCTACC ACCGTGGGCT CCTTCTTGCA TCGGGTGGGG 
GCCCGGGTGC CTCACGCTGT GGTGTGTATT GTAGGCACGC ATGCGGACCT GTGTGGGGAG 
CGGGAGCTTG AGGAGAAATG TCTGGACATT CACCGACAGA TTGCCCTGCA GGAGAAGAAC 
GATGCAGAGG GCCTTAGCCA TCTAGCCAAG GTGGTGGATG AGGCCCTGGC TCGGGACTTC 
GAGCTGCGCT CTGCCAGCCC TCACGCAGCC TACTACGGTG TTTCAGACAA GAACCTTCGG 
AGGCGCAAGG CCCATTTTCA ATACTTGCTT AACCACCGGT TGCAGATCCT CTCTCCCGTG 
TTGCCCGTAA GCTGCCGGGA CCCCCTCCAA TTGCAACGCC TTCGAGACAA GCTGCTCTCT 
GTAGCTGAGC ACCGAGAAAT CTTTCCCAAC TTACACCGAG TCCTGCCTCG ATCCTGGCAG 
GTACTGGAGG AACTGCACTT CCAGCCACCT CAGGCACAAC GACTGTGGCT GAGCTGGTGG 
GACTCTGCCC GCCTGGGCCT GCAGGCAGGT CTGACTGAGG ACCGGCTCCA GAGTGCTCTT 
TCCTACCTGC ACGAGAGCGG CAAACTGCTC TATTTTGAGG ATAGCCCAGC CCTCAAGGAG 
CATGTCTTCC ACAACCTCAC CCGCCTCATT GACATCCTCA ATGTCTTTTT CCAGAGAGAT 
GCTTCATTGC TGCTGCATAA ACTGCTCTTA GGGACCAATG GAGAGGGGGA GGGGGAGGGG 
GAAAGTTTCC CCACTATAGC AGTGCCCAGC CCGGGTCAGG ACCCACTCCG GGCCACCCAG 
CTTCATCATT ATGTGGAAGG CTTTTTGCTG CATGGGCTTC TGCCAGCCCA CATCATTCGG 
TTGCTGCTTA AGCCTCATGT CCAGGCCCAA CAGGACTTGC AGCTCCTGCT GGAGCTGTTG 
GAGAAGATGG GACTCTGTTA CTGCCTCAAT AAACCCAAAG GCAAGCCTTT GAATGGCTCC 
GCGGCATGGT ACAAGTTCCC ATGCTACGTG CAGAATGAGG TGCCCCATGC AGAGGCCTGG 
ATTAATGGCA CCAACCTAGC CGGGCAGTCT TTTGTGGCTG AACAGTTACA GATCGAATAT 
AGTTTTCCCT TCACCTTTCC ACCTGGATTG TTCGCACGTT ACAGTGTCCA GATCAACAGT 
CACGTGGTGC ACAGGTCGGA TGGGAAATTT CAGATCTTTG CATATAGAGG GAAAGTTCCC 
GTGGTGGTGA GTTACAGACC TGCCAAGGGG GTTCTGCAGC CCGACACCCT GTCCATTGCC 
AGCCATGCGT CACTACCAAA TATATGGACC GCATGGCAAG CCATCACCCC TTTGGTAGAG 
GAACTGAATG TCCTACTTCA GGAATGGCCT GGACTGCACT ACACTGTGCA CATCCTCTGT 
TCTAAGTGCC TTAAGAGAGG GTCGCCAAAT CCACACGCTT TCCCAGGGGA GTTGCTGAGC 
CAGCCCAGGC CAGAAGGGGT GGCAGAGATC ATCTGCCCCA AGAATGGCAG CGAGCGAGTG 
AATGTGGCTT TGGTTTACCC ACCTACACCG ACTGTGATCA GCCCTTGTTC CAAGAAGAAC 
GTTGGTGAGA AGCACAGAAA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074748.1
CDS	576..3722
Translation	MAGQDSGNLKTVRLWRDAALRARKLRSNLRQLTLSCPGAGGDPLESPDAPQLVLPANIGDIEVLNLGNNGLEDVPEGLGSALGSLRVLVLRRNRFARLPPAVAELGHHLTELDVSHNRLTILGAEVVSALRELRKLNLSHNQLPALPAQLGALAHLEELDVSFNRLAHLPDSFSCLNHLRTLDVDHNQLTAFPQQLLQLAALEELDVSSNRLRGLPEDISALRALKILWLSGAELGTLPRGFCELASLESLMLDNNGLQALPDEFSRLQRLKMLNLSSNLFEEFPAALLPLAGLEELYLSRNQLTSVPSLIAGLGRLLTLWLDNNRIRYLPDSIVELTGLEELVLQGNQIAVLPDNFGQLSRVGLWKIKDNPLIQPPYEVCMKGIPYIAAYQKELAHSQPAVQPRLKLLLMGHKAAGKTLLRHCLTEDKVEGGQGGGDKEKSYLPFPPLGSKGIEVTSWTADASRGLRFIVYDLAGDESYEVIQPFFLSPGALYVLVVNLATYEPRCFPTTVGSFLHRVGARVPHAVVCIVGTHADLCGERELEEKCLDIHRQIALQEKNDAEGLSHLAKVVDEALARDFELRSASPHAAYYGVSDKNLRRRKAHFQYLLNHRLQILSPVLPVSCRDPLQLQRLRDKLLSVAEHREIFPNLHRVLPRSWQVLEELHFQPPQAQRLWLSWWDSARLGLQAGLTEDRLQSALSYLHESGKLLYFEDSPALKEHVFHNLTRLIDILNVFFQRDASLLLHKLLLGTNGEGEGEGESFPTIAVPSPGQDPLRATQLHHYVEGFLLHGLLPAHIIRLLLKPHVQAQQDLQLLLELLEKMGLCYCLNKPKGKPLNGSAAWYKFPCYVQNEVPHAEAWINGTNLAGQSFVAEQLQIEYSFPFTFPPGLFARYSVQINSHVVHRSDGKFQIFAYRGKVPVVVSYRPAKGVLQPDTLSIASHASLPNIWTAWQAITPLVEELNVLLQEWPGLHYTVHILCSKCLKRGSPNPHAFPGELLSQPRPEGVAEIICPKNGSERVNVALVYPPTPTVISPCSKKNVGEKHRNQ

Target ORF information:

RefSeq Version	NM_001081279.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus malignant fibrous histiocytoma amplified sequence 1 (Mfhas1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081279.1

ORF Insert Sequence:

ATGGCTGGCC AGGACAGTGG CAACCTGAAG ACGGTGAGGC TGTGGCGGGA CGCCGCCCTG 
AGAGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TCAGCTGCCC AGGGGCCGGA 
GGCGACCCAC TGGAGTCCCC CGACGCCCCC CAGCTGGTGC TGCCGGCCAA CATCGGGGAC 
ATTGAGGTGC TGAACCTGGG GAACAACGGC CTCGAGGATG TGCCTGAAGG TCTGGGGTCA 
GCTCTGGGCA GCCTTCGCGT CTTGGTCTTG CGCAGGAACC GTTTTGCCCG GCTGCCCCCT 
GCGGTGGCGG AGCTAGGCCA CCACCTTACC GAACTGGACG TCAGCCACAA CCGGTTGACC 
ATCCTGGGAG CCGAGGTGGT GAGCGCCCTG AGGGAGCTGC GCAAACTCAA CCTGAGCCAC 
AACCAGTTGC CAGCCCTGCC TGCCCAGCTG GGTGCCCTCG CCCACCTGGA GGAGCTGGAT 
GTCAGCTTCA ACCGGCTGGC GCATCTGCCA GATTCCTTCT CCTGCCTCAA CCACCTTCGG 
ACCCTTGACG TGGACCACAA CCAGCTCACC GCCTTTCCCC AGCAGCTGCT GCAGCTGGCT 
GCCCTGGAGG AGCTCGACGT CTCCAGCAAC CGGCTGCGAG GCCTACCTGA GGATATCAGT 
GCCCTGCGTG CCCTCAAGAT CCTCTGGCTT AGCGGGGCCG AGCTTGGCAC GCTGCCCAGA 
GGCTTCTGCG AGCTGGCCAG TTTGGAGAGC CTCATGTTAG ATAACAACGG GCTCCAGGCT 
CTGCCCGACG AGTTCAGCCG TCTGCAGAGG CTCAAAATGC TCAACCTCTC TTCCAACCTC 
TTCGAGGAGT TCCCTGCTGC GCTGCTGCCC CTGGCTGGTC TGGAGGAGCT CTATCTTAGT 
CGCAATCAGC TCACCTCAGT GCCCTCTCTT ATCGCCGGGC TGGGTCGGCT TCTCACCTTG 
TGGCTGGATA ATAACCGCAT CCGGTACCTG CCAGACTCCA TTGTGGAGCT GACTGGCCTG 
GAGGAGCTGG TGCTCCAGGG CAACCAGATC GCTGTGCTGC CGGACAACTT TGGCCAGCTC 
TCCCGGGTAG GCTTGTGGAA AATCAAGGAC AACCCACTGA TTCAGCCCCC TTACGAAGTC 
TGCATGAAGG GGATCCCTTA CATCGCAGCC TACCAGAAGG AACTGGCGCA TTCGCAGCCA 
GCCGTGCAGC CCCGGCTCAA GCTGCTCCTG ATGGGCCACA AGGCTGCAGG AAAGACCCTG 
CTTCGACATT GCCTCACCGA GGACAAAGTG GAGGGAGGGC AAGGTGGAGG GGACAAGGAG 
AAGAGCTACC TACCTTTCCC TCCCCTTGGT AGTAAGGGCA TCGAGGTGAC CAGCTGGACA 
GCGGATGCTT CCCGGGGCTT GCGGTTCATT GTGTATGACT TAGCCGGTGA TGAAAGCTAT 
GAGGTGATAC AGCCCTTCTT CCTCTCCCCT GGAGCCCTGT ATGTGCTCGT GGTCAACTTG 
GCCACCTATG AACCTCGCTG CTTCCCTACC ACCGTGGGCT CCTTCTTGCA TCGGGTGGGG 
GCCCGGGTGC CTCACGCTGT GGTGTGTATT GTAGGCACGC ATGCGGACCT GTGTGGGGAG 
CGGGAGCTTG AGGAGAAATG TCTGGACATT CACCGACAGA TTGCCCTGCA GGAGAAGAAC 
GATGCAGAGG GCCTTAGCCA TCTAGCCAAG GTGGTGGATG AGGCCCTGGC TCGGGACTTC 
GAGCTGCGCT CTGCCAGCCC TCACGCAGCC TACTACGGTG TTTCAGACAA GAACCTTCGG 
AGGCGCAAGG CCCATTTTCA ATACTTGCTT AACCACCGGT TGCAGATCCT CTCTCCCGTG 
TTGCCCGTAA GCTGCCGGGA CCCCCTCCAA TTGCAACGCC TTCGAGACAA GCTGCTCTCT 
GTAGCTGAGC ACCGAGAAAT CTTTCCCAAC TTACACCGAG TCCTGCCTCG ATCCTGGCAG 
GTACTGGAGG AACTGCACTT CCAGCCACCT CAGGCACAAC GACTGTGGCT GAGCTGGTGG 
GACTCTGCCC GCCTGGGCCT GCAGGCAGGT CTGACTGAGG ACCGGCTCCA GAGTGCTCTT 
TCCTACCTGC ACGAGAGCGG CAAACTGCTC TATTTTGAGG ATAGCCCAGC CCTCAAGGAG 
CATGTCTTCC ACAACCTCAC CCGCCTCATT GACATCCTCA ATGTCTTTTT CCAGAGAGAT 
GCTTCATTGC TGCTGCATAA ACTGCTCTTA GGGACCAATG GAGAGGGGGA GGGGGAGGGG 
GAAAGTTTCC CCACTATAGC AGTGCCCAGC CCGGGTCAGG ACCCACTCCG GGCCACCCAG 
CTTCATCATT ATGTGGAAGG CTTTTTGCTG CATGGGCTTC TGCCAGCCCA CATCATTCGG 
TTGCTGCTTA AGCCTCATGT CCAGGCCCAA CAGGACTTGC AGCTCCTGCT GGAGCTGTTG 
GAGAAGATGG GACTCTGTTA CTGCCTCAAT AAACCCAAAG GCAAGCCTTT GAATGGCTCC 
GCGGCATGGT ACAAGTTCCC ATGCTACGTG CAGAATGAGG TGCCCCATGC AGAGGCCTGG 
ATTAATGGCA CCAACCTAGC CGGGCAGTCT TTTGTGGCTG AACAGTTACA GATCGAATAT 
AGTTTTCCCT TCACCTTTCC ACCTGGATTG TTCGCACGTT ACAGTGTCCA GATCAACAGT 
CACGTGGTGC ACAGGTCGGA TGGGAAATTT CAGATCTTTG CATATAGAGG GAAAGTTCCC 
GTGGTGGTGA GTTACAGACC TGCCAAGGGG GTTCTGCAGC CCGACACCCT GTCCATTGCC 
AGCCATGCGT CACTACCAAA TATATGGACC GCATGGCAAG CCATCACCCC TTTGGTAGAG 
GAACTGAATG TCCTACTTCA GGAATGGCCT GGACTGCACT ACACTGTGCA CATCCTCTGT 
TCTAAGTGCC TTAAGAGAGG GTCGCCAAAT CCACACGCTT TCCCAGGGGA GTTGCTGAGC 
CAGCCCAGGC CAGAAGGGGT GGCAGAGATC ATCTGCCCCA AGAATGGCAG CGAGCGAGTG 
AATGTGGCTT TGGTTTACCC ACCTACACCG ACTGTGATCA GCCCTTGTTC CAAGAAGAAC 
GTTGGTGAGA AGCACAGAAA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfhas1 ( NM_001081279.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074748.1 Mus musculus malignant fibrous histiocytoma amplified sequence 1 (Mfhas1), transcript variant 1, mRNA.

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CloneID	OMu02019
Clone ID Related Accession (Same CDS sequence)	NM_001081279.1 , NM_001081279.2
Accession Version	NM_001081279.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Mus musculus(house mouse)
Product	malignant fibrous histiocytoma-amplified sequence 1 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC129082.6 and AC129211.3. On Jun 12, 2018 this sequence version replaced NM_001081279.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC082308.1, AK171731.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGCC AGGACAGTGG CAACCTGAAG ACGGTGAGGC TGTGGCGGGA CGCCGCCCTG 
AGAGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TCAGCTGCCC AGGGGCCGGA 
GGCGACCCAC TGGAGTCCCC CGACGCCCCC CAGCTGGTGC TGCCGGCCAA CATCGGGGAC 
ATTGAGGTGC TGAACCTGGG GAACAACGGC CTCGAGGATG TGCCTGAAGG TCTGGGGTCA 
GCTCTGGGCA GCCTTCGCGT CTTGGTCTTG CGCAGGAACC GTTTTGCCCG GCTGCCCCCT 
GCGGTGGCGG AGCTAGGCCA CCACCTTACC GAACTGGACG TCAGCCACAA CCGGTTGACC 
ATCCTGGGAG CCGAGGTGGT GAGCGCCCTG AGGGAGCTGC GCAAACTCAA CCTGAGCCAC 
AACCAGTTGC CAGCCCTGCC TGCCCAGCTG GGTGCCCTCG CCCACCTGGA GGAGCTGGAT 
GTCAGCTTCA ACCGGCTGGC GCATCTGCCA GATTCCTTCT CCTGCCTCAA CCACCTTCGG 
ACCCTTGACG TGGACCACAA CCAGCTCACC GCCTTTCCCC AGCAGCTGCT GCAGCTGGCT 
GCCCTGGAGG AGCTCGACGT CTCCAGCAAC CGGCTGCGAG GCCTACCTGA GGATATCAGT 
GCCCTGCGTG CCCTCAAGAT CCTCTGGCTT AGCGGGGCCG AGCTTGGCAC GCTGCCCAGA 
GGCTTCTGCG AGCTGGCCAG TTTGGAGAGC CTCATGTTAG ATAACAACGG GCTCCAGGCT 
CTGCCCGACG AGTTCAGCCG TCTGCAGAGG CTCAAAATGC TCAACCTCTC TTCCAACCTC 
TTCGAGGAGT TCCCTGCTGC GCTGCTGCCC CTGGCTGGTC TGGAGGAGCT CTATCTTAGT 
CGCAATCAGC TCACCTCAGT GCCCTCTCTT ATCGCCGGGC TGGGTCGGCT TCTCACCTTG 
TGGCTGGATA ATAACCGCAT CCGGTACCTG CCAGACTCCA TTGTGGAGCT GACTGGCCTG 
GAGGAGCTGG TGCTCCAGGG CAACCAGATC GCTGTGCTGC CGGACAACTT TGGCCAGCTC 
TCCCGGGTAG GCTTGTGGAA AATCAAGGAC AACCCACTGA TTCAGCCCCC TTACGAAGTC 
TGCATGAAGG GGATCCCTTA CATCGCAGCC TACCAGAAGG AACTGGCGCA TTCGCAGCCA 
GCCGTGCAGC CCCGGCTCAA GCTGCTCCTG ATGGGCCACA AGGCTGCAGG AAAGACCCTG 
CTTCGACATT GCCTCACCGA GGACAAAGTG GAGGGAGGGC AAGGTGGAGG GGACAAGGAG 
AAGAGCTACC TACCTTTCCC TCCCCTTGGT AGTAAGGGCA TCGAGGTGAC CAGCTGGACA 
GCGGATGCTT CCCGGGGCTT GCGGTTCATT GTGTATGACT TAGCCGGTGA TGAAAGCTAT 
GAGGTGATAC AGCCCTTCTT CCTCTCCCCT GGAGCCCTGT ATGTGCTCGT GGTCAACTTG 
GCCACCTATG AACCTCGCTG CTTCCCTACC ACCGTGGGCT CCTTCTTGCA TCGGGTGGGG 
GCCCGGGTGC CTCACGCTGT GGTGTGTATT GTAGGCACGC ATGCGGACCT GTGTGGGGAG 
CGGGAGCTTG AGGAGAAATG TCTGGACATT CACCGACAGA TTGCCCTGCA GGAGAAGAAC 
GATGCAGAGG GCCTTAGCCA TCTAGCCAAG GTGGTGGATG AGGCCCTGGC TCGGGACTTC 
GAGCTGCGCT CTGCCAGCCC TCACGCAGCC TACTACGGTG TTTCAGACAA GAACCTTCGG 
AGGCGCAAGG CCCATTTTCA ATACTTGCTT AACCACCGGT TGCAGATCCT CTCTCCCGTG 
TTGCCCGTAA GCTGCCGGGA CCCCCTCCAA TTGCAACGCC TTCGAGACAA GCTGCTCTCT 
GTAGCTGAGC ACCGAGAAAT CTTTCCCAAC TTACACCGAG TCCTGCCTCG ATCCTGGCAG 
GTACTGGAGG AACTGCACTT CCAGCCACCT CAGGCACAAC GACTGTGGCT GAGCTGGTGG 
GACTCTGCCC GCCTGGGCCT GCAGGCAGGT CTGACTGAGG ACCGGCTCCA GAGTGCTCTT 
TCCTACCTGC ACGAGAGCGG CAAACTGCTC TATTTTGAGG ATAGCCCAGC CCTCAAGGAG 
CATGTCTTCC ACAACCTCAC CCGCCTCATT GACATCCTCA ATGTCTTTTT CCAGAGAGAT 
GCTTCATTGC TGCTGCATAA ACTGCTCTTA GGGACCAATG GAGAGGGGGA GGGGGAGGGG 
GAAAGTTTCC CCACTATAGC AGTGCCCAGC CCGGGTCAGG ACCCACTCCG GGCCACCCAG 
CTTCATCATT ATGTGGAAGG CTTTTTGCTG CATGGGCTTC TGCCAGCCCA CATCATTCGG 
TTGCTGCTTA AGCCTCATGT CCAGGCCCAA CAGGACTTGC AGCTCCTGCT GGAGCTGTTG 
GAGAAGATGG GACTCTGTTA CTGCCTCAAT AAACCCAAAG GCAAGCCTTT GAATGGCTCC 
GCGGCATGGT ACAAGTTCCC ATGCTACGTG CAGAATGAGG TGCCCCATGC AGAGGCCTGG 
ATTAATGGCA CCAACCTAGC CGGGCAGTCT TTTGTGGCTG AACAGTTACA GATCGAATAT 
AGTTTTCCCT TCACCTTTCC ACCTGGATTG TTCGCACGTT ACAGTGTCCA GATCAACAGT 
CACGTGGTGC ACAGGTCGGA TGGGAAATTT CAGATCTTTG CATATAGAGG GAAAGTTCCC 
GTGGTGGTGA GTTACAGACC TGCCAAGGGG GTTCTGCAGC CCGACACCCT GTCCATTGCC 
AGCCATGCGT CACTACCAAA TATATGGACC GCATGGCAAG CCATCACCCC TTTGGTAGAG 
GAACTGAATG TCCTACTTCA GGAATGGCCT GGACTGCACT ACACTGTGCA CATCCTCTGT 
TCTAAGTGCC TTAAGAGAGG GTCGCCAAAT CCACACGCTT TCCCAGGGGA GTTGCTGAGC 
CAGCCCAGGC CAGAAGGGGT GGCAGAGATC ATCTGCCCCA AGAATGGCAG CGAGCGAGTG 
AATGTGGCTT TGGTTTACCC ACCTACACCG ACTGTGATCA GCCCTTGTTC CAAGAAGAAC 
GTTGGTGAGA AGCACAGAAA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074748.1
CDS	585..3731
Translation	MAGQDSGNLKTVRLWRDAALRARKLRSNLRQLTLSCPGAGGDPLESPDAPQLVLPANIGDIEVLNLGNNGLEDVPEGLGSALGSLRVLVLRRNRFARLPPAVAELGHHLTELDVSHNRLTILGAEVVSALRELRKLNLSHNQLPALPAQLGALAHLEELDVSFNRLAHLPDSFSCLNHLRTLDVDHNQLTAFPQQLLQLAALEELDVSSNRLRGLPEDISALRALKILWLSGAELGTLPRGFCELASLESLMLDNNGLQALPDEFSRLQRLKMLNLSSNLFEEFPAALLPLAGLEELYLSRNQLTSVPSLIAGLGRLLTLWLDNNRIRYLPDSIVELTGLEELVLQGNQIAVLPDNFGQLSRVGLWKIKDNPLIQPPYEVCMKGIPYIAAYQKELAHSQPAVQPRLKLLLMGHKAAGKTLLRHCLTEDKVEGGQGGGDKEKSYLPFPPLGSKGIEVTSWTADASRGLRFIVYDLAGDESYEVIQPFFLSPGALYVLVVNLATYEPRCFPTTVGSFLHRVGARVPHAVVCIVGTHADLCGERELEEKCLDIHRQIALQEKNDAEGLSHLAKVVDEALARDFELRSASPHAAYYGVSDKNLRRRKAHFQYLLNHRLQILSPVLPVSCRDPLQLQRLRDKLLSVAEHREIFPNLHRVLPRSWQVLEELHFQPPQAQRLWLSWWDSARLGLQAGLTEDRLQSALSYLHESGKLLYFEDSPALKEHVFHNLTRLIDILNVFFQRDASLLLHKLLLGTNGEGEGEGESFPTIAVPSPGQDPLRATQLHHYVEGFLLHGLLPAHIIRLLLKPHVQAQQDLQLLLELLEKMGLCYCLNKPKGKPLNGSAAWYKFPCYVQNEVPHAEAWINGTNLAGQSFVAEQLQIEYSFPFTFPPGLFARYSVQINSHVVHRSDGKFQIFAYRGKVPVVVSYRPAKGVLQPDTLSIASHASLPNIWTAWQAITPLVEELNVLLQEWPGLHYTVHILCSKCLKRGSPNPHAFPGELLSQPRPEGVAEIICPKNGSERVNVALVYPPTPTVISPCSKKNVGEKHRNQ

Target ORF information:

RefSeq Version	NM_001081279.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus malignant fibrous histiocytoma amplified sequence 1 (Mfhas1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081279.2

ORF Insert Sequence:

ATGGCTGGCC AGGACAGTGG CAACCTGAAG ACGGTGAGGC TGTGGCGGGA CGCCGCCCTG 
AGAGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TCAGCTGCCC AGGGGCCGGA 
GGCGACCCAC TGGAGTCCCC CGACGCCCCC CAGCTGGTGC TGCCGGCCAA CATCGGGGAC 
ATTGAGGTGC TGAACCTGGG GAACAACGGC CTCGAGGATG TGCCTGAAGG TCTGGGGTCA 
GCTCTGGGCA GCCTTCGCGT CTTGGTCTTG CGCAGGAACC GTTTTGCCCG GCTGCCCCCT 
GCGGTGGCGG AGCTAGGCCA CCACCTTACC GAACTGGACG TCAGCCACAA CCGGTTGACC 
ATCCTGGGAG CCGAGGTGGT GAGCGCCCTG AGGGAGCTGC GCAAACTCAA CCTGAGCCAC 
AACCAGTTGC CAGCCCTGCC TGCCCAGCTG GGTGCCCTCG CCCACCTGGA GGAGCTGGAT 
GTCAGCTTCA ACCGGCTGGC GCATCTGCCA GATTCCTTCT CCTGCCTCAA CCACCTTCGG 
ACCCTTGACG TGGACCACAA CCAGCTCACC GCCTTTCCCC AGCAGCTGCT GCAGCTGGCT 
GCCCTGGAGG AGCTCGACGT CTCCAGCAAC CGGCTGCGAG GCCTACCTGA GGATATCAGT 
GCCCTGCGTG CCCTCAAGAT CCTCTGGCTT AGCGGGGCCG AGCTTGGCAC GCTGCCCAGA 
GGCTTCTGCG AGCTGGCCAG TTTGGAGAGC CTCATGTTAG ATAACAACGG GCTCCAGGCT 
CTGCCCGACG AGTTCAGCCG TCTGCAGAGG CTCAAAATGC TCAACCTCTC TTCCAACCTC 
TTCGAGGAGT TCCCTGCTGC GCTGCTGCCC CTGGCTGGTC TGGAGGAGCT CTATCTTAGT 
CGCAATCAGC TCACCTCAGT GCCCTCTCTT ATCGCCGGGC TGGGTCGGCT TCTCACCTTG 
TGGCTGGATA ATAACCGCAT CCGGTACCTG CCAGACTCCA TTGTGGAGCT GACTGGCCTG 
GAGGAGCTGG TGCTCCAGGG CAACCAGATC GCTGTGCTGC CGGACAACTT TGGCCAGCTC 
TCCCGGGTAG GCTTGTGGAA AATCAAGGAC AACCCACTGA TTCAGCCCCC TTACGAAGTC 
TGCATGAAGG GGATCCCTTA CATCGCAGCC TACCAGAAGG AACTGGCGCA TTCGCAGCCA 
GCCGTGCAGC CCCGGCTCAA GCTGCTCCTG ATGGGCCACA AGGCTGCAGG AAAGACCCTG 
CTTCGACATT GCCTCACCGA GGACAAAGTG GAGGGAGGGC AAGGTGGAGG GGACAAGGAG 
AAGAGCTACC TACCTTTCCC TCCCCTTGGT AGTAAGGGCA TCGAGGTGAC CAGCTGGACA 
GCGGATGCTT CCCGGGGCTT GCGGTTCATT GTGTATGACT TAGCCGGTGA TGAAAGCTAT 
GAGGTGATAC AGCCCTTCTT CCTCTCCCCT GGAGCCCTGT ATGTGCTCGT GGTCAACTTG 
GCCACCTATG AACCTCGCTG CTTCCCTACC ACCGTGGGCT CCTTCTTGCA TCGGGTGGGG 
GCCCGGGTGC CTCACGCTGT GGTGTGTATT GTAGGCACGC ATGCGGACCT GTGTGGGGAG 
CGGGAGCTTG AGGAGAAATG TCTGGACATT CACCGACAGA TTGCCCTGCA GGAGAAGAAC 
GATGCAGAGG GCCTTAGCCA TCTAGCCAAG GTGGTGGATG AGGCCCTGGC TCGGGACTTC 
GAGCTGCGCT CTGCCAGCCC TCACGCAGCC TACTACGGTG TTTCAGACAA GAACCTTCGG 
AGGCGCAAGG CCCATTTTCA ATACTTGCTT AACCACCGGT TGCAGATCCT CTCTCCCGTG 
TTGCCCGTAA GCTGCCGGGA CCCCCTCCAA TTGCAACGCC TTCGAGACAA GCTGCTCTCT 
GTAGCTGAGC ACCGAGAAAT CTTTCCCAAC TTACACCGAG TCCTGCCTCG ATCCTGGCAG 
GTACTGGAGG AACTGCACTT CCAGCCACCT CAGGCACAAC GACTGTGGCT GAGCTGGTGG 
GACTCTGCCC GCCTGGGCCT GCAGGCAGGT CTGACTGAGG ACCGGCTCCA GAGTGCTCTT 
TCCTACCTGC ACGAGAGCGG CAAACTGCTC TATTTTGAGG ATAGCCCAGC CCTCAAGGAG 
CATGTCTTCC ACAACCTCAC CCGCCTCATT GACATCCTCA ATGTCTTTTT CCAGAGAGAT 
GCTTCATTGC TGCTGCATAA ACTGCTCTTA GGGACCAATG GAGAGGGGGA GGGGGAGGGG 
GAAAGTTTCC CCACTATAGC AGTGCCCAGC CCGGGTCAGG ACCCACTCCG GGCCACCCAG 
CTTCATCATT ATGTGGAAGG CTTTTTGCTG CATGGGCTTC TGCCAGCCCA CATCATTCGG 
TTGCTGCTTA AGCCTCATGT CCAGGCCCAA CAGGACTTGC AGCTCCTGCT GGAGCTGTTG 
GAGAAGATGG GACTCTGTTA CTGCCTCAAT AAACCCAAAG GCAAGCCTTT GAATGGCTCC 
GCGGCATGGT ACAAGTTCCC ATGCTACGTG CAGAATGAGG TGCCCCATGC AGAGGCCTGG 
ATTAATGGCA CCAACCTAGC CGGGCAGTCT TTTGTGGCTG AACAGTTACA GATCGAATAT 
AGTTTTCCCT TCACCTTTCC ACCTGGATTG TTCGCACGTT ACAGTGTCCA GATCAACAGT 
CACGTGGTGC ACAGGTCGGA TGGGAAATTT CAGATCTTTG CATATAGAGG GAAAGTTCCC 
GTGGTGGTGA GTTACAGACC TGCCAAGGGG GTTCTGCAGC CCGACACCCT GTCCATTGCC 
AGCCATGCGT CACTACCAAA TATATGGACC GCATGGCAAG CCATCACCCC TTTGGTAGAG 
GAACTGAATG TCCTACTTCA GGAATGGCCT GGACTGCACT ACACTGTGCA CATCCTCTGT 
TCTAAGTGCC TTAAGAGAGG GTCGCCAAAT CCACACGCTT TCCCAGGGGA GTTGCTGAGC 
CAGCCCAGGC CAGAAGGGGT GGCAGAGATC ATCTGCCCCA AGAATGGCAG CGAGCGAGTG 
AATGTGGCTT TGGTTTACCC ACCTACACCG ACTGTGATCA GCCCTTGTTC CAAGAAGAAC 
GTTGGTGAGA AGCACAGAAA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfhas1 ( XM_006509170.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509233.1 Mus musculus malignant fibrous histiocytoma amplified sequence 1 (Mfhas1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu40676
Clone ID Related Accession (Same CDS sequence)	XM_006509170.2
Accession Version	XM_006509170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	malignant fibrous histiocytoma-amplified sequence 1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCC AGGACAGTGG CAACCTGAAG ACGGTGAGGC TGTGGCGGGA CGCCGCCCTG 
AGAGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TCAGCTGCCC AGGGGCCGGA 
GGCGACCCAC TGGAGTCCCC CGACGCCCCC CAGCTGGTGC TGCCGGCCAA CATCGGGGAC 
ATTGAGGTGC TGAACCTGGG GAACAACGGC CTCGAGGATG TGCCTGAAGG TCTGGGGTCA 
GCTCTGGGCA GCCTTCGCGT CTTGGTCTTG CGCAGGAACC GTTTTGCCCG GCTGCCCCCT 
GCGGTGGCGG AGCTAGGCCA CCACCTTACC GAACTGGACG TCAGCCACAA CCGGTTGACC 
ATCCTGGGAG CCGAGGTGGT GAGCGCCCTG AGGGAGCTGC GCAAACTCAA CCTGAGCCAC 
AACCAGTTGC CAGCCCTGCC TGCCCAGCTG GGTGCCCTCG CCCACCTGGA GGAGCTGGAT 
GTCAGCTTCA ACCGGCTGGC GCATCTGCCA GATTCCTTCT CCTGCCTCAA CCACCTTCGG 
ACCCTTGACG TGGACCACAA CCAGCTCACC GCCTTTCCCC AGCAGCTGCT GCAGCTGGCT 
GCCCTGGAGG AGCTCGACGT CTCCAGCAAC CGGCTGCGAG GCCTACCTGA GGATATCAGT 
GCCCTGCGTG CCCTCAAGAT CCTCTGGCTT AGCGGGGCCG AGCTTGGCAC GCTGCCCAGA 
GGCTTCTGCG AGCTGGCCAG TTTGGAGAGC CTCATGTTAG ATAACAACGG GCTCCAGGCT 
CTGCCCGACG AGTTCAGCCG TCTGCAGAGG CTCAAAATGC TCAACCTCTC TTCCAACCTC 
TTCGAGGAGT TCCCTGCTGC GCTGCTGCCC CTGGCTGGTC TGGAGGAGCT CTATCTTAGT 
CGCAATCAGC TCACCTCAGT GCCCTCTCTT ATCGCCGGGC TGGGTCGGCT TCTCACCTTG 
TGGCTGGATA ATAACCGCAT CCGGTACCTG CCAGACTCCA TTGTGGAGCT GACTGGCCTG 
GAGGAGCTGG TGCTCCAGGG CAACCAGATC GCTGTGCTGC CGGACAACTT TGGCCAGCTC 
TCCCGGGTAG GCTTGTGGAA AATCAAGGAC AACCCACTGA TTCAGCCCCC TTACGAAGTC 
TGCATGAAGG GGATCCCTTA CATCGCAGCC TACCAGAAGG AACTGGCGCA TTCGCAGCCA 
GCCGTGCAGC CCCGGCTCAA GCTGCTCCTG ATGGGCCACA AGGCTGCAGG AAAGACCCTG 
CTTCGACATT GCCTCACCGA GGACAAAGTG GAGGGAGGGC AAGGTGGAGG GGACAAGGAG 
AAGAGCTACC TACCTTTCCC TCCCCTTGGT AGTAAGGGCA TCGAGGTGAC CAGCTGGACA 
GCGGATGCTT CCCGGGGCTT GCGGTTCATT GTGTATGACT TAGCCGGTGA TGAAAGCTAT 
GAGGTGATAC AGCCCTTCTT CCTCTCCCCT GGAGCCCTGT ATGTGCTCGT GGTCAACTTG 
GCCACCTATG AACCTCGCTG CTTCCCTACC ACCGTGGGCT CCTTCTTGCA TCGGGTGGGG 
GCCCGGGTGC CTCACGCTGT GGTGTGTATT GTAGGCACGC ATGCGGACCT GTGTGGGGAG 
CGGGAGCTTG AGGAGAAATG TCTGGACATT CACCGACAGA TTGCCCTGCA GGAGAAGAAC 
GATGCAGAGG GCCTTAGCCA TCTAGCCAAG GTGGTGGATG AGGCCCTGGC TCGGGACTTC 
GAGCTGCGCT CTGCCAGCCC TCACGCAGCC TACTACGGTG TTTCAGACAA GAACCTTCGG 
AGGCGCAAGG CCCATTTTCA ATACTTGCTT AACCACCGGT TGCAGATCCT CTCTCCCGTG 
TTGCCCGTAA GCTGCCGGGA CCCCCTCCAA TTGCAACGCC TTCGAGACAA GCTGCTCTCT 
GTAGCTGAGC ACCGAGAAAT CTTTCCCAAC TTACACCGAG TCCTGCCTCG ATCCTGGCAG 
GTACTGGAGG AACTGCACTT CCAGCCACCT CAGGCACAAC GACTGTGGCT GAGCTGGTGG 
GACTCTGCCC GCCTGGGCCT GCAGGCAGGT CTGACTGAGG ACCGGCTCCA GAGTGCTCTT 
TCCTACCTGC ACGAGAGCGG CAAACTGCTC TATTTTGAGG ATAGCCCAGC CCTCAAGGAG 
CATGTCTTCC ACAACCTCAC CCGCCTCATT GACATCCTCA ATGTCTTTTT CCAGAGAGAT 
GCTTCATTGC TGCTGCATAA ACTGCTCTTA GGGACCAATG GAGAGGGGGA GGGGGAGGGG 
GAAAGTTTCC CCACTATAGC AGTGCCCAGC CCGGGTCAGG ACCCACTCCG GGCCACCCAG 
CTTCATCATT ATGTGGAAGG CTTTTTGCTG CATGGGCTTC TGCCAGCCCA CATCATTCGG 
TTGCTGCTTA AGCCTCATGT CCAGGCCCAA CAGGACTTGC AGCTCCTGCT GGAGCTGTTG 
GAGAAGATGG GACTCTGTTA CTGCCTCAAT AAACCCAAAG GCAAGCCTTT GAATGGCTCC 
GCGGCATGGT ACAAGTTCCC ATGCTACGTG CAGAATGAGG TGCCCCATGC AGAGGCCTGG 
ATTAATGGCA CCAACCTAGC CGGGCAGTCT TTTGTGGCTG AACAGTTACA GATCGAATAT 
AGTTTTCCCT TCACCTTTCC ACCTGGATTG TTCGCACGTT ACAGTGTCCA GATCAACAGT 
CACGTGGTGC ACAGGTCGGA TGGGAAATTT CAGATCTTTG CATATAGAGG GAAAGTTCCC 
GTGGTGGTGA GTTACAGACC TGCCAAGGGG GTTCTGCAGC CCGACACCCT GTCCATTGCC 
AGCCATGCGT CACTACCAAA TATATGGACC GCATGGCAAG CCATCACCCC TTTGGTAGAG 
GAACTGAATG TCCTACTTCA GGAATGGCCT GGACTGCACT ACACTGTGCA CATCCTCTGT 
TCTAAGTGCC TTAAGAGAGG GTCGCCAAAT CCACACGCTT TCCCAGGGGA GTTGCTGAGC 
CAGCCCAGGC CAGAAGGGGT GGCAGAGATC ATCTGCCCCA AGAATGGCAG CGAGCGAGTG 
AATGTGGCTT TGGTTTACCC ACCTACACCG ACTGTGATCA GCCCTTGTTC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509233.1
CDS	27..3143
Translation	MAGQDSGNLKTVRLWRDAALRARKLRSNLRQLTLSCPGAGGDPLESPDAPQLVLPANIGDIEVLNLGNNGLEDVPEGLGSALGSLRVLVLRRNRFARLPPAVAELGHHLTELDVSHNRLTILGAEVVSALRELRKLNLSHNQLPALPAQLGALAHLEELDVSFNRLAHLPDSFSCLNHLRTLDVDHNQLTAFPQQLLQLAALEELDVSSNRLRGLPEDISALRALKILWLSGAELGTLPRGFCELASLESLMLDNNGLQALPDEFSRLQRLKMLNLSSNLFEEFPAALLPLAGLEELYLSRNQLTSVPSLIAGLGRLLTLWLDNNRIRYLPDSIVELTGLEELVLQGNQIAVLPDNFGQLSRVGLWKIKDNPLIQPPYEVCMKGIPYIAAYQKELAHSQPAVQPRLKLLLMGHKAAGKTLLRHCLTEDKVEGGQGGGDKEKSYLPFPPLGSKGIEVTSWTADASRGLRFIVYDLAGDESYEVIQPFFLSPGALYVLVVNLATYEPRCFPTTVGSFLHRVGARVPHAVVCIVGTHADLCGERELEEKCLDIHRQIALQEKNDAEGLSHLAKVVDEALARDFELRSASPHAAYYGVSDKNLRRRKAHFQYLLNHRLQILSPVLPVSCRDPLQLQRLRDKLLSVAEHREIFPNLHRVLPRSWQVLEELHFQPPQAQRLWLSWWDSARLGLQAGLTEDRLQSALSYLHESGKLLYFEDSPALKEHVFHNLTRLIDILNVFFQRDASLLLHKLLLGTNGEGEGEGESFPTIAVPSPGQDPLRATQLHHYVEGFLLHGLLPAHIIRLLLKPHVQAQQDLQLLLELLEKMGLCYCLNKPKGKPLNGSAAWYKFPCYVQNEVPHAEAWINGTNLAGQSFVAEQLQIEYSFPFTFPPGLFARYSVQINSHVVHRSDGKFQIFAYRGKVPVVVSYRPAKGVLQPDTLSIASHASLPNIWTAWQAITPLVEELNVLLQEWPGLHYTVHILCSKCLKRGSPNPHAFPGELLSQPRPEGVAEIICPKNGSERVNVALVYPPTPTVISPCSK

Target ORF information:

RefSeq Version	XM_006509170.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus malignant fibrous histiocytoma amplified sequence 1 (Mfhas1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509170.2

ORF Insert Sequence:

ATGGCTGGCC AGGACAGTGG CAACCTGAAG ACGGTGAGGC TGTGGCGGGA CGCCGCCCTG 
AGAGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TCAGCTGCCC AGGGGCCGGA 
GGCGACCCAC TGGAGTCCCC CGACGCCCCC CAGCTGGTGC TGCCGGCCAA CATCGGGGAC 
ATTGAGGTGC TGAACCTGGG GAACAACGGC CTCGAGGATG TGCCTGAAGG TCTGGGGTCA 
GCTCTGGGCA GCCTTCGCGT CTTGGTCTTG CGCAGGAACC GTTTTGCCCG GCTGCCCCCT 
GCGGTGGCGG AGCTAGGCCA CCACCTTACC GAACTGGACG TCAGCCACAA CCGGTTGACC 
ATCCTGGGAG CCGAGGTGGT GAGCGCCCTG AGGGAGCTGC GCAAACTCAA CCTGAGCCAC 
AACCAGTTGC CAGCCCTGCC TGCCCAGCTG GGTGCCCTCG CCCACCTGGA GGAGCTGGAT 
GTCAGCTTCA ACCGGCTGGC GCATCTGCCA GATTCCTTCT CCTGCCTCAA CCACCTTCGG 
ACCCTTGACG TGGACCACAA CCAGCTCACC GCCTTTCCCC AGCAGCTGCT GCAGCTGGCT 
GCCCTGGAGG AGCTCGACGT CTCCAGCAAC CGGCTGCGAG GCCTACCTGA GGATATCAGT 
GCCCTGCGTG CCCTCAAGAT CCTCTGGCTT AGCGGGGCCG AGCTTGGCAC GCTGCCCAGA 
GGCTTCTGCG AGCTGGCCAG TTTGGAGAGC CTCATGTTAG ATAACAACGG GCTCCAGGCT 
CTGCCCGACG AGTTCAGCCG TCTGCAGAGG CTCAAAATGC TCAACCTCTC TTCCAACCTC 
TTCGAGGAGT TCCCTGCTGC GCTGCTGCCC CTGGCTGGTC TGGAGGAGCT CTATCTTAGT 
CGCAATCAGC TCACCTCAGT GCCCTCTCTT ATCGCCGGGC TGGGTCGGCT TCTCACCTTG 
TGGCTGGATA ATAACCGCAT CCGGTACCTG CCAGACTCCA TTGTGGAGCT GACTGGCCTG 
GAGGAGCTGG TGCTCCAGGG CAACCAGATC GCTGTGCTGC CGGACAACTT TGGCCAGCTC 
TCCCGGGTAG GCTTGTGGAA AATCAAGGAC AACCCACTGA TTCAGCCCCC TTACGAAGTC 
TGCATGAAGG GGATCCCTTA CATCGCAGCC TACCAGAAGG AACTGGCGCA TTCGCAGCCA 
GCCGTGCAGC CCCGGCTCAA GCTGCTCCTG ATGGGCCACA AGGCTGCAGG AAAGACCCTG 
CTTCGACATT GCCTCACCGA GGACAAAGTG GAGGGAGGGC AAGGTGGAGG GGACAAGGAG 
AAGAGCTACC TACCTTTCCC TCCCCTTGGT AGTAAGGGCA TCGAGGTGAC CAGCTGGACA 
GCGGATGCTT CCCGGGGCTT GCGGTTCATT GTGTATGACT TAGCCGGTGA TGAAAGCTAT 
GAGGTGATAC AGCCCTTCTT CCTCTCCCCT GGAGCCCTGT ATGTGCTCGT GGTCAACTTG 
GCCACCTATG AACCTCGCTG CTTCCCTACC ACCGTGGGCT CCTTCTTGCA TCGGGTGGGG 
GCCCGGGTGC CTCACGCTGT GGTGTGTATT GTAGGCACGC ATGCGGACCT GTGTGGGGAG 
CGGGAGCTTG AGGAGAAATG TCTGGACATT CACCGACAGA TTGCCCTGCA GGAGAAGAAC 
GATGCAGAGG GCCTTAGCCA TCTAGCCAAG GTGGTGGATG AGGCCCTGGC TCGGGACTTC 
GAGCTGCGCT CTGCCAGCCC TCACGCAGCC TACTACGGTG TTTCAGACAA GAACCTTCGG 
AGGCGCAAGG CCCATTTTCA ATACTTGCTT AACCACCGGT TGCAGATCCT CTCTCCCGTG 
TTGCCCGTAA GCTGCCGGGA CCCCCTCCAA TTGCAACGCC TTCGAGACAA GCTGCTCTCT 
GTAGCTGAGC ACCGAGAAAT CTTTCCCAAC TTACACCGAG TCCTGCCTCG ATCCTGGCAG 
GTACTGGAGG AACTGCACTT CCAGCCACCT CAGGCACAAC GACTGTGGCT GAGCTGGTGG 
GACTCTGCCC GCCTGGGCCT GCAGGCAGGT CTGACTGAGG ACCGGCTCCA GAGTGCTCTT 
TCCTACCTGC ACGAGAGCGG CAAACTGCTC TATTTTGAGG ATAGCCCAGC CCTCAAGGAG 
CATGTCTTCC ACAACCTCAC CCGCCTCATT GACATCCTCA ATGTCTTTTT CCAGAGAGAT 
GCTTCATTGC TGCTGCATAA ACTGCTCTTA GGGACCAATG GAGAGGGGGA GGGGGAGGGG 
GAAAGTTTCC CCACTATAGC AGTGCCCAGC CCGGGTCAGG ACCCACTCCG GGCCACCCAG 
CTTCATCATT ATGTGGAAGG CTTTTTGCTG CATGGGCTTC TGCCAGCCCA CATCATTCGG 
TTGCTGCTTA AGCCTCATGT CCAGGCCCAA CAGGACTTGC AGCTCCTGCT GGAGCTGTTG 
GAGAAGATGG GACTCTGTTA CTGCCTCAAT AAACCCAAAG GCAAGCCTTT GAATGGCTCC 
GCGGCATGGT ACAAGTTCCC ATGCTACGTG CAGAATGAGG TGCCCCATGC AGAGGCCTGG 
ATTAATGGCA CCAACCTAGC CGGGCAGTCT TTTGTGGCTG AACAGTTACA GATCGAATAT 
AGTTTTCCCT TCACCTTTCC ACCTGGATTG TTCGCACGTT ACAGTGTCCA GATCAACAGT 
CACGTGGTGC ACAGGTCGGA TGGGAAATTT CAGATCTTTG CATATAGAGG GAAAGTTCCC 
GTGGTGGTGA GTTACAGACC TGCCAAGGGG GTTCTGCAGC CCGACACCCT GTCCATTGCC 
AGCCATGCGT CACTACCAAA TATATGGACC GCATGGCAAG CCATCACCCC TTTGGTAGAG 
GAACTGAATG TCCTACTTCA GGAATGGCCT GGACTGCACT ACACTGTGCA CATCCTCTGT 
TCTAAGTGCC TTAAGAGAGG GTCGCCAAAT CCACACGCTT TCCCAGGGGA GTTGCTGAGC 
CAGCCCAGGC CAGAAGGGGT GGCAGAGATC ATCTGCCCCA AGAATGGCAG CGAGCGAGTG 
AATGTGGCTT TGGTTTACCC ACCTACACCG ACTGTGATCA GCCCTTGTTC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MFHAS1 promotes colorectal cancer progress by regulating polarization of tumor-associated macrophages via STAT6 signaling pathway.
Oncotarget7(48)78726-78735(2016 Nov)
Chen W,Xu Y,Zhong J,Wang H,Weng M,Cheng Q,Wu Q,Sun Z,Jiang H,Zhu M,Ren Y,Xu P,Chen J,Miao C

MFHAS1 Is Associated with Sepsis and Stimulates TLR2/NF-?B Signaling Pathway Following Negative Regulation.
PloS one10(11)e0143662(2015)
Zhong J,Shi QQ,Zhu MM,Shen J,Wang HH,Ma D,Miao CH

Human leucine-rich repeat proteins: a genome-wide bioinformatic categorization and functional analysis in innate immunity.
Proceedings of the National Academy of Sciences of the United States of America108 Suppl 14631-8(2011 Mar)
Ng AC,Eisenberg JM,Heath RJ,Huett A,Robinson CM,Nau GJ,Xavier RJ

A high-resolution anatomical atlas of the transcriptome in the mouse embryo.
PLoS biology9(1)e1000582(2011 Jan)
Diez-Roux G,Banfi S,Sultan M,Geffers L,Anand S,Rozado D,Magen A,Canidio E,Pagani M,Peluso I,Lin-Marq N,Koch M,Bilio M,Cantiello I,Verde R,De Masi C,Bianchi SA,Cicchini J,Perroud E,Mehmeti S,Dagand E,Schrinner S,N?rnberger A,Schmidt K,Metz K,Zwingmann C,Brieske N,Springer C,Hernandez AM,Herzog S,Grabbe F,Sieverding C,Fischer B,Schrader K,Brockmeyer M,Dettmer S,Helbig C,Alunni V,Battaini MA,Mura C,Henrichsen CN,Garcia-Lopez R,Echevarria D,Puelles E,Garcia-Calero E,Kruse S,Uhr M,Kauck C,Feng G,Milyaev N,Ong CK,Kumar L,Lam M,Semple CA,Gyenesei A,Mundlos S,Radelof U,Lehrach H,Sarmientos P,Reymond A,Davidson DR,Doll? P,Antonarakis SE,Yaspo ML,Martinez S,Baldock RA,Eichele G,Ballabio A

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Mfhas1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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