Knstrn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Knstrn
Entrez Gene ID	51944
Full Name	kinetochore-localized astrin/SPAG5 binding
Synonyms	1700025D04Rik,C15orf23,D2Ertd750e,SKAP,Traf4af1
General protein information	Preferred Names kinetochore-localized astrin/SPAG5 binding Names small kinetochore-associated protein TRAF4 associated factor 1 homolog TRAF4-associated factor 1 kinastrin kinetochore-localized astrin-binding protein kinetochore-localized astrin/SPAG5-binding protein putative TRAF4-associated factor 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E5

mRNA and Protein(s)

mRNA	Protein	Name
XM_017319123.1	XP_017174612.1	small kinetochore-associated protein isoform X1
NM_026412.3	NP_080688.2	small kinetochore-associated protein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Knstrn	NP_001004264.1
	C7H15orf23	XP_001095787.2
Canis lupus familiaris (dog)	KNSTRN	XP_003640084.1
Pan troglodytes (chimpanzee)	C15H15orf23	NP_001238816.1
Mus musculus (house mouse)	Knstrn	NP_080688.2
Bos taurus (cattle)	C10H15orf23	NP_001033144.1
Homo sapiens (human)	KNSTRN	NP_150628.3

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Characterization of SKAP/kinastrin isoforms: the N-terminus defines tissue specificity and Pontin binding.
Cindric Vranesic A, Reiche J, Hoischen C, Wohlmann A, Bratsch J, Friedrich K, G?nes B, Cappallo-Obermann H, Kirchhoff C, Diekmann S, G?nes C, Huber O
Human molecular genetics25(13)2838-2852(2016 07)

SKAP, an outer kinetochore protein, is required for mouse germ cell development.
Grey C, Espeut J, Ametsitsi R, Kumar R, Luksza M, Brun C, Verlhac MH, Suja JA, de Massy B
Reproduction (Cambridge, England)151(3)239-51(2016 Mar)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Protein-protein interaction panel using mouse full-length cDNAs.
Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y
Genome research11(10)1758-65(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

important role in the successful outcome of male gametogenesis
Title: SKAP, an outer kinetochore protein, is required for mouse germ cell development.

The following Knstrn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Knstrn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02222	NM_026412.3 Latest version!	Mus musculus kinetochore-localized astrin/SPAG5 binding (Knstrn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu80330	XM_017319123.1 Latest version!	Mus musculus kinetochore-localized astrin/SPAG5 binding (Knstrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Knstrn ( NM_026412.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080688.2 Mus musculus kinetochore-localized astrin/SPAG5 binding (Knstrn), mRNA.

Price & Availability↑

CloneID	OMu02222
Clone ID Related Accession (Same CDS sequence)	NM_026412.3
Accession Version	NM_026412.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	small kinetochore-associated protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK006328.1, AK036374.1 and AK028590.1. On Apr 29, 2009 this sequence version replaced NM_026412.2. ##Evidence-Data-START## Transcript exon combination :: AK028590.1, SRR1660817.14208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCTC CCGAGGCCGA GGCACAAGAG ACAGCCTTCC GTACAACAGG GCCGCCTACA 
GACTCGGAGC CGCGCCCCTT CCCTCCCAGC TCCCGGAAGT TTCCTTTTGA AAGCGCGGCG 
GCAGACTCTA ACGAGGACTG GGCAGTTGCT GCGGAGCATT ATTTGAAAGG GAGCGGAGAG 
AACGGCGGAT GGGAGCAGCC GGCACCGGGG GTCCAGCCAA GCCACCCGGC TACGATGGCC 
AGTGCTAAGA CGGTGTGCGA TGCGCAGCCT CACTCGATGC CGAGCTGCGG CTTGCCCGCA 
GATACACAAA CTCGAGCTAC TTCTAAACTA CCTGTTAAAT CCAAAGAAGC GGATTTGCTT 
AGACATCTTC ATCCAGGAGG GCCAGAGCCT GATGTTACAA AAGTCACCAA ATCGAGACGA 
GAGAATGGGC AGGTGAAAGC GGCAGAGACT GCCAGCAGGA GGAACCTCAG AAACAGCTAC 
AAACCGTTTA ATAAGCAAAA ACCGGAGGAG GAACTAAAGG ATAAAAATGA GCTGCTGGAG 
GCTGTCAACA AGCAGTTACA CCAGAAGCTG ACAGAGACTC AGGGAGAGCT GAAGGACCTG 
ACACAGAAAG TGGAGCTACT GGAGAAGTTT CAGGATAACT GCTTAGCACT TTTGGAGAGC 
AAAGGTCTCA ACCCAGGCCA AGAGACCCTG GCATCAAAGC AGGAACCCAC CACAGATCAC 
ACGGACTCCA TGCTGCTGCT CGAAACTTTG AAAGACGAAC TGAAGGTTTT CAATGAAACC 
GCCAAGAAGC AGATGGAGGA GCTACAGGCC TTGAAGGTGA AGTTGAAGCT GAAAGAAGAG 
GAGAGTGTCC AGTTCCTGGA ACAGCAGACC TTATGTAAGG ACGAAGCCAG TGACTTCACA 
ATAATCCTAG AGGAAATGGA GCAGCTCTTA GAAATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080688.2
CDS	99..1037
Translation	MAAPEAEAQETAFRTTGPPTDSEPRPFPPSSRKFPFESAAADSNEDWAVAAEHYLKGSGENGGWEQPAPGVQPSHPATMASAKTVCDAQPHSMPSCGLPADTQTRATSKLPVKSKEADLLRHLHPGGPEPDVTKVTKSRRENGQVKAAETASRRNLRNSYKPFNKQKPEEELKDKNELLEAVNKQLHQKLTETQGELKDLTQKVELLEKFQDNCLALLESKGLNPGQETLASKQEPTTDHTDSMLLLETLKDELKVFNETAKKQMEELQALKVKLKLKEEESVQFLEQQTLCKDEASDFTIILEEMEQLLEM

Target ORF information:

RefSeq Version	NM_026412.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinetochore-localized astrin/SPAG5 binding (Knstrn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026412.3

ORF Insert Sequence:

ATGGCGGCTC CCGAGGCCGA GGCACAAGAG ACAGCCTTCC GTACAACAGG GCCGCCTACA 
GACTCGGAGC CGCGCCCCTT CCCTCCCAGC TCCCGGAAGT TTCCTTTTGA AAGCGCGGCG 
GCAGACTCTA ACGAGGACTG GGCAGTTGCT GCGGAGCATT ATTTGAAAGG GAGCGGAGAG 
AACGGCGGAT GGGAGCAGCC GGCACCGGGG GTCCAGCCAA GCCACCCGGC TACGATGGCC 
AGTGCTAAGA CGGTGTGCGA TGCGCAGCCT CACTCGATGC CGAGCTGCGG CTTGCCCGCA 
GATACACAAA CTCGAGCTAC TTCTAAACTA CCTGTTAAAT CCAAAGAAGC GGATTTGCTT 
AGACATCTTC ATCCAGGAGG GCCAGAGCCT GATGTTACAA AAGTCACCAA ATCGAGACGA 
GAGAATGGGC AGGTGAAAGC GGCAGAGACT GCCAGCAGGA GGAACCTCAG AAACAGCTAC 
AAACCGTTTA ATAAGCAAAA ACCGGAGGAG GAACTAAAGG ATAAAAATGA GCTGCTGGAG 
GCTGTCAACA AGCAGTTACA CCAGAAGCTG ACAGAGACTC AGGGAGAGCT GAAGGACCTG 
ACACAGAAAG TGGAGCTACT GGAGAAGTTT CAGGATAACT GCTTAGCACT TTTGGAGAGC 
AAAGGTCTCA ACCCAGGCCA AGAGACCCTG GCATCAAAGC AGGAACCCAC CACAGATCAC 
ACGGACTCCA TGCTGCTGCT CGAAACTTTG AAAGACGAAC TGAAGGTTTT CAATGAAACC 
GCCAAGAAGC AGATGGAGGA GCTACAGGCC TTGAAGGTGA AGTTGAAGCT GAAAGAAGAG 
GAGAGTGTCC AGTTCCTGGA ACAGCAGACC TTATGTAAGG ACGAAGCCAG TGACTTCACA 
ATAATCCTAG AGGAAATGGA GCAGCTCTTA GAAATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Knstrn ( XM_017319123.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174612.1 Mus musculus kinetochore-localized astrin/SPAG5 binding (Knstrn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu80330
Clone ID Related Accession (Same CDS sequence)	XM_017319123.1
Accession Version	XM_017319123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	small kinetochore-associated protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC CCGAGGCCGA GGCACAAGAG ACAGCCTTCC GTACAACAGG GCCGCCTACA 
GACTCGGAGC CGCGCCCCTT CCCTCCCAGC TCCCGGAAGT TTCCTTTTGA AAGCGCGGCG 
GCAGACTCTA ACGAGGACTG GGCAGTTGCT GCGGAGCATT ATTTGAAAGG GAGCGGAGAG 
AACGGCGGAT GGGAGCAGCC GGCACCGGGG GTCCAGCCAA GCCACCCGGC TACGATGGCC 
AGTGCTAAGA CGGTGTGCGA TGCGCAGCCT CACTCGATGC CGAGCTGCGG CTTGCCCGCA 
GATACACAAA CTCGAGCTAC TTCTAAACTA CCTGTTAAAT CCAAAGAAGC GGATTTGCTT 
AGACATCTTC ATCCAGGAGG GCCAGAGCCT GATGTTACAA AAGTCACCAA ATCGAGACGA 
GAGAATGGGC AGGTGAAAGC GGCAGAGACT GCCAGCAGGA GGAACCTCAG AAACAGCTAC 
AAACCGTTTA ATAAGCAAAA ACCGGAGGAG GAACTAAAGG ATAAAAATGA GCTGCTGGAG 
GCTGTCAACA AGCAGTTACA CCAGAAGCTG ACAGAGACTC AGGGAGAGCT GAAGGACCTG 
ACACAGAAAG TGGAGCTACT GGAGAAGTTT CAGGATAACT GCTTAGCACT TTTGGAGAGC 
AAAGGTCTCA ACCCAGGCCA AGAGACCCTG GCATCAAAGC AGGAACCCAC CACAGATCAC 
ACGGACTCCA TGCTGCTGCT CGAAACTTTG AAAGACGAAC TGAAGGTTTT CAATGAAACC 
GCCAAGAAGC AGATGGAGGA GCTACAGGGT ATGCTTTGCC TAAGTGTGCA CACGTACAGA 
CTGCGACAGT GTCCACGGAG GTTAGAGCTG GGTTACAGGC AGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174612.1
CDS	114..1001
Translation	MAAPEAEAQETAFRTTGPPTDSEPRPFPPSSRKFPFESAAADSNEDWAVAAEHYLKGSGENGGWEQPAPGVQPSHPATMASAKTVCDAQPHSMPSCGLPADTQTRATSKLPVKSKEADLLRHLHPGGPEPDVTKVTKSRRENGQVKAAETASRRNLRNSYKPFNKQKPEEELKDKNELLEAVNKQLHQKLTETQGELKDLTQKVELLEKFQDNCLALLESKGLNPGQETLASKQEPTTDHTDSMLLLETLKDELKVFNETAKKQMEELQGMLCLSVHTYRLRQCPRRLELGYRQL

Target ORF information:

RefSeq Version	XM_017319123.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinetochore-localized astrin/SPAG5 binding (Knstrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319123.1

ORF Insert Sequence:

ATGGCGGCTC CCGAGGCCGA GGCACAAGAG ACAGCCTTCC GTACAACAGG GCCGCCTACA 
GACTCGGAGC CGCGCCCCTT CCCTCCCAGC TCCCGGAAGT TTCCTTTTGA AAGCGCGGCG 
GCAGACTCTA ACGAGGACTG GGCAGTTGCT GCGGAGCATT ATTTGAAAGG GAGCGGAGAG 
AACGGCGGAT GGGAGCAGCC GGCACCGGGG GTCCAGCCAA GCCACCCGGC TACGATGGCC 
AGTGCTAAGA CGGTGTGCGA TGCGCAGCCT CACTCGATGC CGAGCTGCGG CTTGCCCGCA 
GATACACAAA CTCGAGCTAC TTCTAAACTA CCTGTTAAAT CCAAAGAAGC GGATTTGCTT 
AGACATCTTC ATCCAGGAGG GCCAGAGCCT GATGTTACAA AAGTCACCAA ATCGAGACGA 
GAGAATGGGC AGGTGAAAGC GGCAGAGACT GCCAGCAGGA GGAACCTCAG AAACAGCTAC 
AAACCGTTTA ATAAGCAAAA ACCGGAGGAG GAACTAAAGG ATAAAAATGA GCTGCTGGAG 
GCTGTCAACA AGCAGTTACA CCAGAAGCTG ACAGAGACTC AGGGAGAGCT GAAGGACCTG 
ACACAGAAAG TGGAGCTACT GGAGAAGTTT CAGGATAACT GCTTAGCACT TTTGGAGAGC 
AAAGGTCTCA ACCCAGGCCA AGAGACCCTG GCATCAAAGC AGGAACCCAC CACAGATCAC 
ACGGACTCCA TGCTGCTGCT CGAAACTTTG AAAGACGAAC TGAAGGTTTT CAATGAAACC 
GCCAAGAAGC AGATGGAGGA GCTACAGGGT ATGCTTTGCC TAAGTGTGCA CACGTACAGA 
CTGCGACAGT GTCCACGGAG GTTAGAGCTG GGTTACAGGC AGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Characterization of SKAP/kinastrin isoforms: the N-terminus defines tissue specificity and Pontin binding.
Human molecular genetics25(13)2838-2852(2016 07)
Cindric Vranesic A,Reiche J,Hoischen C,Wohlmann A,Bratsch J,Friedrich K,G?nes B,Cappallo-Obermann H,Kirchhoff C,Diekmann S,G?nes C,Huber O

SKAP, an outer kinetochore protein, is required for mouse germ cell development.
Reproduction (Cambridge, England)151(3)239-51(2016 Mar)
Grey C,Espeut J,Ametsitsi R,Kumar R,Luksza M,Brun C,Verlhac MH,Suja JA,de Massy B

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Protein-protein interaction panel using mouse full-length cDNAs.
Genome research11(10)1758-65(2001 Oct)
Suzuki H,Fukunishi Y,Kagawa I,Saito R,Oda H,Endo T,Kondo S,Bono H,Okazaki Y,Hayashizaki Y

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Knstrn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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