Nckap1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nckap1
Entrez Gene ID	50884
Full Name	NCK-associated protein 1
Synonyms	C79304,H19,Hem-2,Hem2,Nap1,mKIAA0587,mh19,p125Nap1
General protein information	Preferred Names NCK-associated protein 1 Names nck-associated protein 1 brain protein H19 membrane-associated protein HEM-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 C3\|2 48.21 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006499882.2	XP_006499945.1	nck-associated protein 1 isoform X2
NM_001355410.1	NP_001342339.1	nck-associated protein 1 isoform c
NM_001290745.1	NP_001277674.1	nck-associated protein 1 isoform a
NM_001355411.1	NP_001342340.1	nck-associated protein 1 isoform d
XM_006499881.3	XP_006499944.1	nck-associated protein 1 isoform X1
NM_016965.3	NP_058661.1	nck-associated protein 1 isoform b

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NCKAP1	XP_535990.3
Xenopus tropicalis (tropical clawed frog)	nckap1	XP_004917966.1
Pan troglodytes (chimpanzee)	NCKAP1	XP_001148485.2
Rattus norvegicus (Norway rat)	Nckap1	NP_113806.1
Drosophila melanogaster (fruit fly)	Hem	NP_524214.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004946	XP_315044.2
	NCKAP1	XP_001099879.2
Bos taurus (cattle)	LOC101907541	XP_005202363.1
Mus musculus (house mouse)	Nckap1	NP_058661.1
Arabidopsis thaliana (thale cress)	GRL	NP_001031485.1
Oryza sativa (rice)	Os08g0544500	NP_001062406.2
Homo sapiens (human)	NCKAP1	NP_995314.1
Gallus gallus (chicken)	NCKAP1	XP_001232275.2
Danio rerio (zebrafish)	nckap1	NP_001131137.1
Caenorhabditis elegans (roundworm)	gex-3	NP_502368.1

Gene Ontology
Bibliography

Related articles in PubMed

NCK Associated Protein 1 Modulated by miRNA-214 Determines Vascular Smooth Muscle Cell Migration, Proliferation, and Neointima Hyperplasia.
Afzal TA, Luong LA, Chen D, Zhang C, Yang F, Chen Q, An W, Wilkes E, Yashiro K, Cutillas PR, Zhang L, Xiao Q
Journal of the American Heart Association5(12)(2016 Dec)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Loss of Sip1 leads to migration defects and retention of ectodermal markers during lens development.
Manthey AL, Lachke SA, FitzGerald PG, Mason RW, Scheiblin DA, McDonald JH, Duncan MK
Mechanisms of development13186-110(2014 Feb)

CYFIP1 coordinates mRNA translation and cytoskeleton remodeling to ensure proper dendritic spine formation.
De Rubeis S, Pasciuto E, Li KW, Fern?ndez E, Di Marino D, Buzzi A, Ostroff LE, Klann E, Zwartkruis FJ, Komiyama NH, Grant SG, Poujol C, Choquet D, Achsel T, Posthuma D, Smit AB, Bagni C
Neuron79(6)1169-82(2013 Sep)

NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons.
Yokoyama K, Tezuka T, Kotani M, Nakazawa T, Hoshina N, Shimoda Y, Kakuta S, Sudo K, Watanabe K, Iwakura Y, Yamamoto T
The EMBO journal30(23)4739-54(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Inhibition of Nap1, a member of the WAVE actin-remodeling complex, results in accumulations of F-actin structures at the plasma membrane that are concomitant with a decrease in myoblast fusion.
Title: Nap1-mediated actin remodeling is essential for mammalian myoblast fusion.

Nap1 mutant phenotypes define the crucial roles of Nap1/WAVE-mediated actin regulation in tissue organization and establishment of the body plan of the mammalian embryo.
Title: Axis specification and morphogenesis in the mouse embryo require Nap1, a regulator of WAVE-mediated actin branching.

Nap1 is a constitutive and essential component of a WAVE2- and Abi-1-containing complex linking Rac to site-directed actin assembly.
Title: Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation.

The following Nckap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nckap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14288	NM_016965.3 Latest version!	Mus musculus NCK-associated protein 1 (Nckap1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu17060	NM_001290745.1 Latest version!	Mus musculus NCK-associated protein 1 (Nckap1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu30690	NM_001355410.1 Latest version!	Mus musculus NCK-associated protein 1 (Nckap1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu30691	NM_001355411.1 Latest version!	Mus musculus NCK-associated protein 1 (Nckap1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu30690	XM_006499881.3 Latest version!	Mus musculus NCK-associated protein 1 (Nckap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu30691	XM_006499882.2 Latest version!	Mus musculus NCK-associated protein 1 (Nckap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nckap1 ( NM_016965.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058661.1 Mus musculus NCK-associated protein 1 (Nckap1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14288
Clone ID Related Accession (Same CDS sequence)	NM_016965.3
Accession Version	NM_016965.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	nck-associated protein 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU055892.1, BC060197.1, AJ534525.1 and BP764873.1. On Mar 19, 2014 this sequence version replaced NM_016965.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' end compared to variant 1, resulting in an isoform (b) that is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060197.1, SRR1660817.106317.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACGCGC CTCTACAACA TCAAGAAGGC ATGTGGCGAC 
CCCAAGGCTA AACCCTCCTA CCTTATTGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TTCCTGCTGT AGAAACGCGC AACAACAATC AACAGCTGGC ACAGCTACAG 
AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCGTTGTATT ACTTTACATT TGTAGATGTT 
ATGGAATTTA AGGACCATGT CTGTGACTTG CTGAATACTA TTGACGTTTG CCAAGTCTTC 
TTTGATATTA CTGTGAACTT TGATTTAACA AAGAACTACT TAGACTTGAC TGTAACCTAT 
ACAACATTAA TGATACTGCT GTCCCGGATT GAAGAACGGA AGGCAATCAT TGGACTATAC 
AATTATGCAC ATGAAATGAC CCATGGAGCA AGTGACCGAG AATATCCACG CCTTGGTCAG 
ATGATTGTGG ACTATGAAAA CCCTTTAAAA AAGATGATGG AAGAATTTGT ACCCCATAGC 
AAGTCTCTTT CAGATGCGCT AATTTCTCTT CAGATGGTGT ATCCTAGACG GAACCTTTCA 
GCAGACCAGT GGAGGAATGC CCAGTTGCTG AGTCTCATCA GTGCACCCAG CACAATGCTT 
AATCCTGCGC AGTCGGACAC TATGCCTTGT GAATATCTCT CCTTGGATGC AATGGAGAAA 
TGGATCATCT TTGGCTTTAT TCTGTGCCAT GGGATGCTAA ATACGGAGGC TACCGCACTG 
AACCTTTGGA AGCTAGCTCT TCAGAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT 
TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TTCGGGGCTA CAATAAACGT 
ATTAACGACA TAAGAGAATG CAAGGAGGCG GCTGTATCCC ACGCCGGCTC AATGCACAGA 
GAAAGACGCA AGTTTTTACG GTCTGCACTA AAAGAACTGG CCACTGTCCT CTCTGACCAG 
CCTGGCCTGC TTGGTCCCAA GGCACTTTTT GTTTTCATGG CGTTATCTTT TGCCCGTGAT 
GAAATTATCT GGCTGCTCCG TCACGCGGAC AACATGCCAA AGAAGAGTGC AGATGACTTT 
ATAGATAAGC ACATTGCTGA GCTAATATTT TACATGGAAG AGCTTAGAGC ACATGTCAGG 
AAGTATGGGC CTGTCATGCA GAGGTACTAC GTGCAATACC TCTCTGGCTT TGATGCTGTT 
GTCCTCAATG AACTTGTGCA GAACCTGTCT GTTTGCCCTG AGGATGAATC GATCATCATG 
TCCTCTTTTG TGAACACCAT GACCTCCCTA AGTGTGAAGC AAGTTGAAGA TGGAGAAGTG 
TTTGATTTCA GAGGGATGAG ATTAGATTGG TTTAGGTTAC AGGCGTACAC TAGCGTCTCC 
AAAGCTTCAC TTAGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA CACAATCATA 
TTCCATACAA AAATGGTAGA TTCCTTGGTT GAAATGTTGG TGGAAACATC CGATCTCTCC 
ATATTTTGTT TTTACAGCCG TGCTTTTGAG AAGATGTTTC AGCAGTGCTT GGAGCTGCCC 
TCTCAGTCAA GATACTCCAT TGCCTTTCCT CTGCTCTGCA CTCACTTCAT GAGCTGCACA 
CATGAACTGT GTCCTGAAGA GCGCCATCAT ATAGGAGATC GTAGCCTTTC CCTGTGCAAT 
ATGTTCCTGG ATGAGATGGC CAAACAAGCT CGAAATCTCA TCACCGATAT TTGCACAGAA 
CAGTGTACTC TTAGTGACCA GTTATTGCCG AAGCATTGTG CCAAAACCAT CAGTCAAGCA 
GTGAATAAGA AGTCAAAAAA ACAGACGGGC AAGAAAGGGG AACCTGAAAG GGAAAAACCG 
GGTGTGGAGA GCATGAGGAA AAACAGGCTG GTAGTGACCA ACCTTGATAA GTTGCACACT 
GCACTTTCGG AGTTATGCTT CTCCATAAAT TATGTTCCAA ACATGGCGGT GTGGGAGCAC 
ACCTTCACGC CGAGGGAGTA TTTGACTTCT CATCTGGAAA TCCGGTTCAC TAAATCAATT 
GTTGGAATGA CTATGTATAA TCAAGCTACA CAGGAAATTG CAAAGCCATC AGAGCTTCTA 
ACAAGCGTAA GAGCATATAT GACTGTACTC CAGTCCATAG AGAACTATGT GCAGATTGAT 
ATCACCAGAG TATTTAATAA TGTCCTTCTT CAGCAAACAC AACACTTAGA CAGCCATGGA 
GAACCAACCA TCACGAGTCT GTATACAAAC TGGTACTTGG AAACTTTATT AAGACAAGTC 
AGCAATGGCC ATATAGCTTA TTTTCCTGCA ATGAAAGCAT TTGTGAATTT ACCCACAGAA 
AATGAATTAA CATTCAATGC AGAGGAATAT TCTGATATAT CAGAAATGAG ATCATTATCA 
GAGCTCCTAG GCCCATATGG GATGAAGTTC CTGAGTGAGA GCCTTATGTG GCACATTTCA 
TCACAAGTAG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTTCT AACACAAATG 
AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTCT TTAAAAGATT ATCATCTGTT 
GACAGTGTCT TGAAAAGGAT GACGATAATT GGTGTTATTT TATCCTTCCG GTCATTGGCA 
CAAGAAGCAT TGAGAGATGT CCTGTCCTAC CACATTCCTT TCCTCGTGAG CTCCATTGAA 
GATTTCAAGG ATCACATTCC TCGGGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG 
CTGTCCTCGG CTGCTGGGTT ACCATGTGAG ATTGATCCTG CCTTAGTCGT AGCTCTTTCC 
TCACAAAAAT CAGAGAACAT TAGTCCAGAG GAAGAATACA AGATCGCTTG TCTCCTGATG 
GTCTTTGTAG CAGTTTCCTT GCCTACACTG GCCAGCAATG TAATGTCACA GTACAGCCCT 
GCAATAGAAG GACACTGCAA CAATATACAT TGTTTGGCCA AAGCCATCAA CCAGATTGCT 
GCAGCTTTGT TTACAATTCA CAAAGGAAGT ATTGAAGACC GACTTAAGGA ATTCCTGGCG 
CTTGCATCAT CCAGTCTACT GAAAATTGGC CAAGAGACAG ATAAAACTAC AACAAGAAAT 
AGAGAATCTG TTTATTTGCT ACTGGATATG ATTGTACAAG AATCACCATT CCTGACAATG 
GATCTCTTGG AATCTTGTTT TCCTTATGTG TTGCTGAGAA ATGCATACCA TGCTGTCTAC 
AAACAAAGTG TTACATCTTC TGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058661.1
CDS	218..3604
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCDLLNTIDVCQVFFDITVNFDLTKNYLDLTVTYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGMLNTEATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMAVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	NM_016965.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 1 (Nckap1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016965.3

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACGCGC CTCTACAACA TCAAGAAGGC ATGTGGCGAC 
CCCAAGGCTA AACCCTCCTA CCTTATTGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TTCCTGCTGT AGAAACGCGC AACAACAATC AACAGCTGGC ACAGCTACAG 
AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCGTTGTATT ACTTTACATT TGTAGATGTT 
ATGGAATTTA AGGACCATGT CTGTGACTTG CTGAATACTA TTGACGTTTG CCAAGTCTTC 
TTTGATATTA CTGTGAACTT TGATTTAACA AAGAACTACT TAGACTTGAC TGTAACCTAT 
ACAACATTAA TGATACTGCT GTCCCGGATT GAAGAACGGA AGGCAATCAT TGGACTATAC 
AATTATGCAC ATGAAATGAC CCATGGAGCA AGTGACCGAG AATATCCACG CCTTGGTCAG 
ATGATTGTGG ACTATGAAAA CCCTTTAAAA AAGATGATGG AAGAATTTGT ACCCCATAGC 
AAGTCTCTTT CAGATGCGCT AATTTCTCTT CAGATGGTGT ATCCTAGACG GAACCTTTCA 
GCAGACCAGT GGAGGAATGC CCAGTTGCTG AGTCTCATCA GTGCACCCAG CACAATGCTT 
AATCCTGCGC AGTCGGACAC TATGCCTTGT GAATATCTCT CCTTGGATGC AATGGAGAAA 
TGGATCATCT TTGGCTTTAT TCTGTGCCAT GGGATGCTAA ATACGGAGGC TACCGCACTG 
AACCTTTGGA AGCTAGCTCT TCAGAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT 
TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TTCGGGGCTA CAATAAACGT 
ATTAACGACA TAAGAGAATG CAAGGAGGCG GCTGTATCCC ACGCCGGCTC AATGCACAGA 
GAAAGACGCA AGTTTTTACG GTCTGCACTA AAAGAACTGG CCACTGTCCT CTCTGACCAG 
CCTGGCCTGC TTGGTCCCAA GGCACTTTTT GTTTTCATGG CGTTATCTTT TGCCCGTGAT 
GAAATTATCT GGCTGCTCCG TCACGCGGAC AACATGCCAA AGAAGAGTGC AGATGACTTT 
ATAGATAAGC ACATTGCTGA GCTAATATTT TACATGGAAG AGCTTAGAGC ACATGTCAGG 
AAGTATGGGC CTGTCATGCA GAGGTACTAC GTGCAATACC TCTCTGGCTT TGATGCTGTT 
GTCCTCAATG AACTTGTGCA GAACCTGTCT GTTTGCCCTG AGGATGAATC GATCATCATG 
TCCTCTTTTG TGAACACCAT GACCTCCCTA AGTGTGAAGC AAGTTGAAGA TGGAGAAGTG 
TTTGATTTCA GAGGGATGAG ATTAGATTGG TTTAGGTTAC AGGCGTACAC TAGCGTCTCC 
AAAGCTTCAC TTAGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA CACAATCATA 
TTCCATACAA AAATGGTAGA TTCCTTGGTT GAAATGTTGG TGGAAACATC CGATCTCTCC 
ATATTTTGTT TTTACAGCCG TGCTTTTGAG AAGATGTTTC AGCAGTGCTT GGAGCTGCCC 
TCTCAGTCAA GATACTCCAT TGCCTTTCCT CTGCTCTGCA CTCACTTCAT GAGCTGCACA 
CATGAACTGT GTCCTGAAGA GCGCCATCAT ATAGGAGATC GTAGCCTTTC CCTGTGCAAT 
ATGTTCCTGG ATGAGATGGC CAAACAAGCT CGAAATCTCA TCACCGATAT TTGCACAGAA 
CAGTGTACTC TTAGTGACCA GTTATTGCCG AAGCATTGTG CCAAAACCAT CAGTCAAGCA 
GTGAATAAGA AGTCAAAAAA ACAGACGGGC AAGAAAGGGG AACCTGAAAG GGAAAAACCG 
GGTGTGGAGA GCATGAGGAA AAACAGGCTG GTAGTGACCA ACCTTGATAA GTTGCACACT 
GCACTTTCGG AGTTATGCTT CTCCATAAAT TATGTTCCAA ACATGGCGGT GTGGGAGCAC 
ACCTTCACGC CGAGGGAGTA TTTGACTTCT CATCTGGAAA TCCGGTTCAC TAAATCAATT 
GTTGGAATGA CTATGTATAA TCAAGCTACA CAGGAAATTG CAAAGCCATC AGAGCTTCTA 
ACAAGCGTAA GAGCATATAT GACTGTACTC CAGTCCATAG AGAACTATGT GCAGATTGAT 
ATCACCAGAG TATTTAATAA TGTCCTTCTT CAGCAAACAC AACACTTAGA CAGCCATGGA 
GAACCAACCA TCACGAGTCT GTATACAAAC TGGTACTTGG AAACTTTATT AAGACAAGTC 
AGCAATGGCC ATATAGCTTA TTTTCCTGCA ATGAAAGCAT TTGTGAATTT ACCCACAGAA 
AATGAATTAA CATTCAATGC AGAGGAATAT TCTGATATAT CAGAAATGAG ATCATTATCA 
GAGCTCCTAG GCCCATATGG GATGAAGTTC CTGAGTGAGA GCCTTATGTG GCACATTTCA 
TCACAAGTAG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTTCT AACACAAATG 
AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTCT TTAAAAGATT ATCATCTGTT 
GACAGTGTCT TGAAAAGGAT GACGATAATT GGTGTTATTT TATCCTTCCG GTCATTGGCA 
CAAGAAGCAT TGAGAGATGT CCTGTCCTAC CACATTCCTT TCCTCGTGAG CTCCATTGAA 
GATTTCAAGG ATCACATTCC TCGGGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG 
CTGTCCTCGG CTGCTGGGTT ACCATGTGAG ATTGATCCTG CCTTAGTCGT AGCTCTTTCC 
TCACAAAAAT CAGAGAACAT TAGTCCAGAG GAAGAATACA AGATCGCTTG TCTCCTGATG 
GTCTTTGTAG CAGTTTCCTT GCCTACACTG GCCAGCAATG TAATGTCACA GTACAGCCCT 
GCAATAGAAG GACACTGCAA CAATATACAT TGTTTGGCCA AAGCCATCAA CCAGATTGCT 
GCAGCTTTGT TTACAATTCA CAAAGGAAGT ATTGAAGACC GACTTAAGGA ATTCCTGGCG 
CTTGCATCAT CCAGTCTACT GAAAATTGGC CAAGAGACAG ATAAAACTAC AACAAGAAAT 
AGAGAATCTG TTTATTTGCT ACTGGATATG ATTGTACAAG AATCACCATT CCTGACAATG 
GATCTCTTGG AATCTTGTTT TCCTTATGTG TTGCTGAGAA ATGCATACCA TGCTGTCTAC 
AAACAAAGTG TTACATCTTC TGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap1 ( NM_001290745.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277674.1 Mus musculus NCK-associated protein 1 (Nckap1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu17060
Clone ID Related Accession (Same CDS sequence)	NM_001290745.1
Accession Version	NM_001290745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	nck-associated protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU055892.1, BC060197.1, BC127067.1 and BP764873.1. On Mar 19, 2014 this sequence version replaced XM_006499880.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.4894.1, SRR1660821.27408.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACGCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGCGACCC CAAGGCTAAA CCCTCCTACC TTATTGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTT CCTGCTGTAG AAACGCGCAA CAACAATCAA 
CAGCTGGCAC AGCTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC GTTGTATTAC 
TTTACATTTG TAGATGTTAT GGAATTTAAG GACCATGTCT GTGACTTGCT GAATACTATT 
GACGTTTGCC AAGTCTTCTT TGATATTACT GTGAACTTTG ATTTAACAAA GAACTACTTA 
GACTTGACTG TAACCTATAC AACATTAATG ATACTGCTGT CCCGGATTGA AGAACGGAAG 
GCAATCATTG GACTATACAA TTATGCACAT GAAATGACCC ATGGAGCAAG TGACCGAGAA 
TATCCACGCC TTGGTCAGAT GATTGTGGAC TATGAAAACC CTTTAAAAAA GATGATGGAA 
GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCGCTAA TTTCTCTTCA GATGGTGTAT 
CCTAGACGGA ACCTTTCAGC AGACCAGTGG AGGAATGCCC AGTTGCTGAG TCTCATCAGT 
GCACCCAGCA CAATGCTTAA TCCTGCGCAG TCGGACACTA TGCCTTGTGA ATATCTCTCC 
TTGGATGCAA TGGAGAAATG GATCATCTTT GGCTTTATTC TGTGCCATGG GATGCTAAAT 
ACGGAGGCTA CCGCACTGAA CCTTTGGAAG CTAGCTCTTC AGAGTAGCTC TTGCCTCTCT 
CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATT 
CGGGGCTACA ATAAACGTAT TAACGACATA AGAGAATGCA AGGAGGCGGC TGTATCCCAC 
GCCGGCTCAA TGCACAGAGA AAGACGCAAG TTTTTACGGT CTGCACTAAA AGAACTGGCC 
ACTGTCCTCT CTGACCAGCC TGGCCTGCTT GGTCCCAAGG CACTTTTTGT TTTCATGGCG 
TTATCTTTTG CCCGTGATGA AATTATCTGG CTGCTCCGTC ACGCGGACAA CATGCCAAAG 
AAGAGTGCAG ATGACTTTAT AGATAAGCAC ATTGCTGAGC TAATATTTTA CATGGAAGAG 
CTTAGAGCAC ATGTCAGGAA GTATGGGCCT GTCATGCAGA GGTACTACGT GCAATACCTC 
TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTTGTGCAGA ACCTGTCTGT TTGCCCTGAG 
GATGAATCGA TCATCATGTC CTCTTTTGTG AACACCATGA CCTCCCTAAG TGTGAAGCAA 
GTTGAAGATG GAGAAGTGTT TGATTTCAGA GGGATGAGAT TAGATTGGTT TAGGTTACAG 
GCGTACACTA GCGTCTCCAA AGCTTCACTT AGCCTTGCAG ATCACAGAGA ACTTGGAAAG 
ATGATGAACA CAATCATATT CCATACAAAA ATGGTAGATT CCTTGGTTGA AATGTTGGTG 
GAAACATCCG ATCTCTCCAT ATTTTGTTTT TACAGCCGTG CTTTTGAGAA GATGTTTCAG 
CAGTGCTTGG AGCTGCCCTC TCAGTCAAGA TACTCCATTG CCTTTCCTCT GCTCTGCACT 
CACTTCATGA GCTGCACACA TGAACTGTGT CCTGAAGAGC GCCATCATAT AGGAGATCGT 
AGCCTTTCCC TGTGCAATAT GTTCCTGGAT GAGATGGCCA AACAAGCTCG AAATCTCATC 
ACCGATATTT GCACAGAACA GTGTACTCTT AGTGACCAGT TATTGCCGAA GCATTGTGCC 
AAAACCATCA GTCAAGCAGT GAATAAGAAG TCAAAAAAAC AGACGGGCAA GAAAGGGGAA 
CCTGAAAGGG AAAAACCGGG TGTGGAGAGC ATGAGGAAAA ACAGGCTGGT AGTGACCAAC 
CTTGATAAGT TGCACACTGC ACTTTCGGAG TTATGCTTCT CCATAAATTA TGTTCCAAAC 
ATGGCGGTGT GGGAGCACAC CTTCACGCCG AGGGAGTATT TGACTTCTCA TCTGGAAATC 
CGGTTCACTA AATCAATTGT TGGAATGACT ATGTATAATC AAGCTACACA GGAAATTGCA 
AAGCCATCAG AGCTTCTAAC AAGCGTAAGA GCATATATGA CTGTACTCCA GTCCATAGAG 
AACTATGTGC AGATTGATAT CACCAGAGTA TTTAATAATG TCCTTCTTCA GCAAACACAA 
CACTTAGACA GCCATGGAGA ACCAACCATC ACGAGTCTGT ATACAAACTG GTACTTGGAA 
ACTTTATTAA GACAAGTCAG CAATGGCCAT ATAGCTTATT TTCCTGCAAT GAAAGCATTT 
GTGAATTTAC CCACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGATATATCA 
GAAATGAGAT CATTATCAGA GCTCCTAGGC CCATATGGGA TGAAGTTCCT GAGTGAGAGC 
CTTATGTGGC ACATTTCATC ACAAGTAGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT 
GATGTTCTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTCTTT 
AAAAGATTAT CATCTGTTGA CAGTGTCTTG AAAAGGATGA CGATAATTGG TGTTATTTTA 
TCCTTCCGGT CATTGGCACA AGAAGCATTG AGAGATGTCC TGTCCTACCA CATTCCTTTC 
CTCGTGAGCT CCATTGAAGA TTTCAAGGAT CACATTCCTC GGGAAACTGA TATGAAGGTT 
GCAATGAATG TGTATGAGCT GTCCTCGGCT GCTGGGTTAC CATGTGAGAT TGATCCTGCC 
TTAGTCGTAG CTCTTTCCTC ACAAAAATCA GAGAACATTA GTCCAGAGGA AGAATACAAG 
ATCGCTTGTC TCCTGATGGT CTTTGTAGCA GTTTCCTTGC CTACACTGGC CAGCAATGTA 
ATGTCACAGT ACAGCCCTGC AATAGAAGGA CACTGCAACA ATATACATTG TTTGGCCAAA 
GCCATCAACC AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGTAT TGAAGACCGA 
CTTAAGGAAT TCCTGGCGCT TGCATCATCC AGTCTACTGA AAATTGGCCA AGAGACAGAT 
AAAACTACAA CAAGAAATAG AGAATCTGTT TATTTGCTAC TGGATATGAT TGTACAAGAA 
TCACCATTCC TGACAATGGA TCTCTTGGAA TCTTGTTTTC CTTATGTGTT GCTGAGAAAT 
GCATACCATG CTGTCTACAA ACAAAGTGTT ACATCTTCTG CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277674.1
CDS	218..3622
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCDLLNTIDVCQVFFDITVNFDLTKNYLDLTVTYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGMLNTEATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMAVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	NM_001290745.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 1 (Nckap1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290745.1

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACGCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGCGACCC CAAGGCTAAA CCCTCCTACC TTATTGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTT CCTGCTGTAG AAACGCGCAA CAACAATCAA 
CAGCTGGCAC AGCTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC GTTGTATTAC 
TTTACATTTG TAGATGTTAT GGAATTTAAG GACCATGTCT GTGACTTGCT GAATACTATT 
GACGTTTGCC AAGTCTTCTT TGATATTACT GTGAACTTTG ATTTAACAAA GAACTACTTA 
GACTTGACTG TAACCTATAC AACATTAATG ATACTGCTGT CCCGGATTGA AGAACGGAAG 
GCAATCATTG GACTATACAA TTATGCACAT GAAATGACCC ATGGAGCAAG TGACCGAGAA 
TATCCACGCC TTGGTCAGAT GATTGTGGAC TATGAAAACC CTTTAAAAAA GATGATGGAA 
GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCGCTAA TTTCTCTTCA GATGGTGTAT 
CCTAGACGGA ACCTTTCAGC AGACCAGTGG AGGAATGCCC AGTTGCTGAG TCTCATCAGT 
GCACCCAGCA CAATGCTTAA TCCTGCGCAG TCGGACACTA TGCCTTGTGA ATATCTCTCC 
TTGGATGCAA TGGAGAAATG GATCATCTTT GGCTTTATTC TGTGCCATGG GATGCTAAAT 
ACGGAGGCTA CCGCACTGAA CCTTTGGAAG CTAGCTCTTC AGAGTAGCTC TTGCCTCTCT 
CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATT 
CGGGGCTACA ATAAACGTAT TAACGACATA AGAGAATGCA AGGAGGCGGC TGTATCCCAC 
GCCGGCTCAA TGCACAGAGA AAGACGCAAG TTTTTACGGT CTGCACTAAA AGAACTGGCC 
ACTGTCCTCT CTGACCAGCC TGGCCTGCTT GGTCCCAAGG CACTTTTTGT TTTCATGGCG 
TTATCTTTTG CCCGTGATGA AATTATCTGG CTGCTCCGTC ACGCGGACAA CATGCCAAAG 
AAGAGTGCAG ATGACTTTAT AGATAAGCAC ATTGCTGAGC TAATATTTTA CATGGAAGAG 
CTTAGAGCAC ATGTCAGGAA GTATGGGCCT GTCATGCAGA GGTACTACGT GCAATACCTC 
TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTTGTGCAGA ACCTGTCTGT TTGCCCTGAG 
GATGAATCGA TCATCATGTC CTCTTTTGTG AACACCATGA CCTCCCTAAG TGTGAAGCAA 
GTTGAAGATG GAGAAGTGTT TGATTTCAGA GGGATGAGAT TAGATTGGTT TAGGTTACAG 
GCGTACACTA GCGTCTCCAA AGCTTCACTT AGCCTTGCAG ATCACAGAGA ACTTGGAAAG 
ATGATGAACA CAATCATATT CCATACAAAA ATGGTAGATT CCTTGGTTGA AATGTTGGTG 
GAAACATCCG ATCTCTCCAT ATTTTGTTTT TACAGCCGTG CTTTTGAGAA GATGTTTCAG 
CAGTGCTTGG AGCTGCCCTC TCAGTCAAGA TACTCCATTG CCTTTCCTCT GCTCTGCACT 
CACTTCATGA GCTGCACACA TGAACTGTGT CCTGAAGAGC GCCATCATAT AGGAGATCGT 
AGCCTTTCCC TGTGCAATAT GTTCCTGGAT GAGATGGCCA AACAAGCTCG AAATCTCATC 
ACCGATATTT GCACAGAACA GTGTACTCTT AGTGACCAGT TATTGCCGAA GCATTGTGCC 
AAAACCATCA GTCAAGCAGT GAATAAGAAG TCAAAAAAAC AGACGGGCAA GAAAGGGGAA 
CCTGAAAGGG AAAAACCGGG TGTGGAGAGC ATGAGGAAAA ACAGGCTGGT AGTGACCAAC 
CTTGATAAGT TGCACACTGC ACTTTCGGAG TTATGCTTCT CCATAAATTA TGTTCCAAAC 
ATGGCGGTGT GGGAGCACAC CTTCACGCCG AGGGAGTATT TGACTTCTCA TCTGGAAATC 
CGGTTCACTA AATCAATTGT TGGAATGACT ATGTATAATC AAGCTACACA GGAAATTGCA 
AAGCCATCAG AGCTTCTAAC AAGCGTAAGA GCATATATGA CTGTACTCCA GTCCATAGAG 
AACTATGTGC AGATTGATAT CACCAGAGTA TTTAATAATG TCCTTCTTCA GCAAACACAA 
CACTTAGACA GCCATGGAGA ACCAACCATC ACGAGTCTGT ATACAAACTG GTACTTGGAA 
ACTTTATTAA GACAAGTCAG CAATGGCCAT ATAGCTTATT TTCCTGCAAT GAAAGCATTT 
GTGAATTTAC CCACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGATATATCA 
GAAATGAGAT CATTATCAGA GCTCCTAGGC CCATATGGGA TGAAGTTCCT GAGTGAGAGC 
CTTATGTGGC ACATTTCATC ACAAGTAGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT 
GATGTTCTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTCTTT 
AAAAGATTAT CATCTGTTGA CAGTGTCTTG AAAAGGATGA CGATAATTGG TGTTATTTTA 
TCCTTCCGGT CATTGGCACA AGAAGCATTG AGAGATGTCC TGTCCTACCA CATTCCTTTC 
CTCGTGAGCT CCATTGAAGA TTTCAAGGAT CACATTCCTC GGGAAACTGA TATGAAGGTT 
GCAATGAATG TGTATGAGCT GTCCTCGGCT GCTGGGTTAC CATGTGAGAT TGATCCTGCC 
TTAGTCGTAG CTCTTTCCTC ACAAAAATCA GAGAACATTA GTCCAGAGGA AGAATACAAG 
ATCGCTTGTC TCCTGATGGT CTTTGTAGCA GTTTCCTTGC CTACACTGGC CAGCAATGTA 
ATGTCACAGT ACAGCCCTGC AATAGAAGGA CACTGCAACA ATATACATTG TTTGGCCAAA 
GCCATCAACC AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGTAT TGAAGACCGA 
CTTAAGGAAT TCCTGGCGCT TGCATCATCC AGTCTACTGA AAATTGGCCA AGAGACAGAT 
AAAACTACAA CAAGAAATAG AGAATCTGTT TATTTGCTAC TGGATATGAT TGTACAAGAA 
TCACCATTCC TGACAATGGA TCTCTTGGAA TCTTGTTTTC CTTATGTGTT GCTGAGAAAT 
GCATACCATG CTGTCTACAA ACAAAGTGTT ACATCTTCTG CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap1 ( NM_001355410.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001342339.1 Mus musculus NCK-associated protein 1 (Nckap1), transcript variant 3, mRNA.

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CloneID	OMu30690
Clone ID Related Accession (Same CDS sequence)	NM_001355410.1 , XM_006499881.3
Accession Version	NM_001355410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	nck-associated protein 1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL928578.6. On Sep 20, 2017 this sequence version replaced XM_006499881.3. Transcript Variant: This variant (3) uses an alternate in-frame splice junction in the 5' end compared to variant 1, resulting in an isoform (c) that is shorter compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.236273.1, SRR1660811.43205.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACGCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGCGACCC CAAGGCTAAA CCCTCCTACC TTATTGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTT CCTGCTGTAG AAACGCGCAA CAACAATCTG 
GCACAGCTAC AGAAAGAAAA ATCAGAGATT CTGAAAAATC TGGCGTTGTA TTACTTTACA 
TTTGTAGATG TTATGGAATT TAAGGACCAT GTCTGTGACT TGCTGAATAC TATTGACGTT 
TGCCAAGTCT TCTTTGATAT TACTGTGAAC TTTGATTTAA CAAAGAACTA CTTAGACTTG 
ACTGTAACCT ATACAACATT AATGATACTG CTGTCCCGGA TTGAAGAACG GAAGGCAATC 
ATTGGACTAT ACAATTATGC ACATGAAATG ACCCATGGAG CAAGTGACCG AGAATATCCA 
CGCCTTGGTC AGATGATTGT GGACTATGAA AACCCTTTAA AAAAGATGAT GGAAGAATTT 
GTACCCCATA GCAAGTCTCT TTCAGATGCG CTAATTTCTC TTCAGATGGT GTATCCTAGA 
CGGAACCTTT CAGCAGACCA GTGGAGGAAT GCCCAGTTGC TGAGTCTCAT CAGTGCACCC 
AGCACAATGC TTAATCCTGC GCAGTCGGAC ACTATGCCTT GTGAATATCT CTCCTTGGAT 
GCAATGGAGA AATGGATCAT CTTTGGCTTT ATTCTGTGCC ATGGGATGCT AAATACGGAG 
GCTACCGCAC TGAACCTTTG GAAGCTAGCT CTTCAGAGTA GCTCTTGCCT CTCTCTCTTT 
CGGGATGAAG TTTTCCACAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATTCGGGGC 
TACAATAAAC GTATTAACGA CATAAGAGAA TGCAAGGAGG CGGCTGTATC CCACGCCGGC 
TCAATGCACA GAGAAAGACG CAAGTTTTTA CGGTCTGCAC TAAAAGAACT GGCCACTGTC 
CTCTCTGACC AGCCTGGCCT GCTTGGTCCC AAGGCACTTT TTGTTTTCAT GGCGTTATCT 
TTTGCCCGTG ATGAAATTAT CTGGCTGCTC CGTCACGCGG ACAACATGCC AAAGAAGAGT 
GCAGATGACT TTATAGATAA GCACATTGCT GAGCTAATAT TTTACATGGA AGAGCTTAGA 
GCACATGTCA GGAAGTATGG GCCTGTCATG CAGAGGTACT ACGTGCAATA CCTCTCTGGC 
TTTGATGCTG TTGTCCTCAA TGAACTTGTG CAGAACCTGT CTGTTTGCCC TGAGGATGAA 
TCGATCATCA TGTCCTCTTT TGTGAACACC ATGACCTCCC TAAGTGTGAA GCAAGTTGAA 
GATGGAGAAG TGTTTGATTT CAGAGGGATG AGATTAGATT GGTTTAGGTT ACAGGCGTAC 
ACTAGCGTCT CCAAAGCTTC ACTTAGCCTT GCAGATCACA GAGAACTTGG AAAGATGATG 
AACACAATCA TATTCCATAC AAAAATGGTA GATTCCTTGG TTGAAATGTT GGTGGAAACA 
TCCGATCTCT CCATATTTTG TTTTTACAGC CGTGCTTTTG AGAAGATGTT TCAGCAGTGC 
TTGGAGCTGC CCTCTCAGTC AAGATACTCC ATTGCCTTTC CTCTGCTCTG CACTCACTTC 
ATGAGCTGCA CACATGAACT GTGTCCTGAA GAGCGCCATC ATATAGGAGA TCGTAGCCTT 
TCCCTGTGCA ATATGTTCCT GGATGAGATG GCCAAACAAG CTCGAAATCT CATCACCGAT 
ATTTGCACAG AACAGTGTAC TCTTAGTGAC CAGTTATTGC CGAAGCATTG TGCCAAAACC 
ATCAGTCAAG CAGTGAATAA GAAGTCAAAA AAACAGACGG GCAAGAAAGG GGAACCTGAA 
AGGGAAAAAC CGGGTGTGGA GAGCATGAGG AAAAACAGGC TGGTAGTGAC CAACCTTGAT 
AAGTTGCACA CTGCACTTTC GGAGTTATGC TTCTCCATAA ATTATGTTCC AAACATGGCG 
GTGTGGGAGC ACACCTTCAC GCCGAGGGAG TATTTGACTT CTCATCTGGA AATCCGGTTC 
ACTAAATCAA TTGTTGGAAT GACTATGTAT AATCAAGCTA CACAGGAAAT TGCAAAGCCA 
TCAGAGCTTC TAACAAGCGT AAGAGCATAT ATGACTGTAC TCCAGTCCAT AGAGAACTAT 
GTGCAGATTG ATATCACCAG AGTATTTAAT AATGTCCTTC TTCAGCAAAC ACAACACTTA 
GACAGCCATG GAGAACCAAC CATCACGAGT CTGTATACAA ACTGGTACTT GGAAACTTTA 
TTAAGACAAG TCAGCAATGG CCATATAGCT TATTTTCCTG CAATGAAAGC ATTTGTGAAT 
TTACCCACAG AAAATGAATT AACATTCAAT GCAGAGGAAT ATTCTGATAT ATCAGAAATG 
AGATCATTAT CAGAGCTCCT AGGCCCATAT GGGATGAAGT TCCTGAGTGA GAGCCTTATG 
TGGCACATTT CATCACAAGT AGCTGAACTT AAGAAACTTG TGGTGGAGAA TGTTGATGTT 
CTAACACAAA TGAGGACCAG CTTTGACAAA CCAGACCAGA TGGCTGCACT CTTTAAAAGA 
TTATCATCTG TTGACAGTGT CTTGAAAAGG ATGACGATAA TTGGTGTTAT TTTATCCTTC 
CGGTCATTGG CACAAGAAGC ATTGAGAGAT GTCCTGTCCT ACCACATTCC TTTCCTCGTG 
AGCTCCATTG AAGATTTCAA GGATCACATT CCTCGGGAAA CTGATATGAA GGTTGCAATG 
AATGTGTATG AGCTGTCCTC GGCTGCTGGG TTACCATGTG AGATTGATCC TGCCTTAGTC 
GTAGCTCTTT CCTCACAAAA ATCAGAGAAC ATTAGTCCAG AGGAAGAATA CAAGATCGCT 
TGTCTCCTGA TGGTCTTTGT AGCAGTTTCC TTGCCTACAC TGGCCAGCAA TGTAATGTCA 
CAGTACAGCC CTGCAATAGA AGGACACTGC AACAATATAC ATTGTTTGGC CAAAGCCATC 
AACCAGATTG CTGCAGCTTT GTTTACAATT CACAAAGGAA GTATTGAAGA CCGACTTAAG 
GAATTCCTGG CGCTTGCATC ATCCAGTCTA CTGAAAATTG GCCAAGAGAC AGATAAAACT 
ACAACAAGAA ATAGAGAATC TGTTTATTTG CTACTGGATA TGATTGTACA AGAATCACCA 
TTCCTGACAA TGGATCTCTT GGAATCTTGT TTTCCTTATG TGTTGCTGAG AAATGCATAC 
CATGCTGTCT ACAAACAAAG TGTTACATCT TCTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342339.1
CDS	218..3616
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCDLLNTIDVCQVFFDITVNFDLTKNYLDLTVTYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGMLNTEATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMAVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	NM_001355410.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 1 (Nckap1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355410.1

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACGCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGCGACCC CAAGGCTAAA CCCTCCTACC TTATTGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTT CCTGCTGTAG AAACGCGCAA CAACAATCTG 
GCACAGCTAC AGAAAGAAAA ATCAGAGATT CTGAAAAATC TGGCGTTGTA TTACTTTACA 
TTTGTAGATG TTATGGAATT TAAGGACCAT GTCTGTGACT TGCTGAATAC TATTGACGTT 
TGCCAAGTCT TCTTTGATAT TACTGTGAAC TTTGATTTAA CAAAGAACTA CTTAGACTTG 
ACTGTAACCT ATACAACATT AATGATACTG CTGTCCCGGA TTGAAGAACG GAAGGCAATC 
ATTGGACTAT ACAATTATGC ACATGAAATG ACCCATGGAG CAAGTGACCG AGAATATCCA 
CGCCTTGGTC AGATGATTGT GGACTATGAA AACCCTTTAA AAAAGATGAT GGAAGAATTT 
GTACCCCATA GCAAGTCTCT TTCAGATGCG CTAATTTCTC TTCAGATGGT GTATCCTAGA 
CGGAACCTTT CAGCAGACCA GTGGAGGAAT GCCCAGTTGC TGAGTCTCAT CAGTGCACCC 
AGCACAATGC TTAATCCTGC GCAGTCGGAC ACTATGCCTT GTGAATATCT CTCCTTGGAT 
GCAATGGAGA AATGGATCAT CTTTGGCTTT ATTCTGTGCC ATGGGATGCT AAATACGGAG 
GCTACCGCAC TGAACCTTTG GAAGCTAGCT CTTCAGAGTA GCTCTTGCCT CTCTCTCTTT 
CGGGATGAAG TTTTCCACAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATTCGGGGC 
TACAATAAAC GTATTAACGA CATAAGAGAA TGCAAGGAGG CGGCTGTATC CCACGCCGGC 
TCAATGCACA GAGAAAGACG CAAGTTTTTA CGGTCTGCAC TAAAAGAACT GGCCACTGTC 
CTCTCTGACC AGCCTGGCCT GCTTGGTCCC AAGGCACTTT TTGTTTTCAT GGCGTTATCT 
TTTGCCCGTG ATGAAATTAT CTGGCTGCTC CGTCACGCGG ACAACATGCC AAAGAAGAGT 
GCAGATGACT TTATAGATAA GCACATTGCT GAGCTAATAT TTTACATGGA AGAGCTTAGA 
GCACATGTCA GGAAGTATGG GCCTGTCATG CAGAGGTACT ACGTGCAATA CCTCTCTGGC 
TTTGATGCTG TTGTCCTCAA TGAACTTGTG CAGAACCTGT CTGTTTGCCC TGAGGATGAA 
TCGATCATCA TGTCCTCTTT TGTGAACACC ATGACCTCCC TAAGTGTGAA GCAAGTTGAA 
GATGGAGAAG TGTTTGATTT CAGAGGGATG AGATTAGATT GGTTTAGGTT ACAGGCGTAC 
ACTAGCGTCT CCAAAGCTTC ACTTAGCCTT GCAGATCACA GAGAACTTGG AAAGATGATG 
AACACAATCA TATTCCATAC AAAAATGGTA GATTCCTTGG TTGAAATGTT GGTGGAAACA 
TCCGATCTCT CCATATTTTG TTTTTACAGC CGTGCTTTTG AGAAGATGTT TCAGCAGTGC 
TTGGAGCTGC CCTCTCAGTC AAGATACTCC ATTGCCTTTC CTCTGCTCTG CACTCACTTC 
ATGAGCTGCA CACATGAACT GTGTCCTGAA GAGCGCCATC ATATAGGAGA TCGTAGCCTT 
TCCCTGTGCA ATATGTTCCT GGATGAGATG GCCAAACAAG CTCGAAATCT CATCACCGAT 
ATTTGCACAG AACAGTGTAC TCTTAGTGAC CAGTTATTGC CGAAGCATTG TGCCAAAACC 
ATCAGTCAAG CAGTGAATAA GAAGTCAAAA AAACAGACGG GCAAGAAAGG GGAACCTGAA 
AGGGAAAAAC CGGGTGTGGA GAGCATGAGG AAAAACAGGC TGGTAGTGAC CAACCTTGAT 
AAGTTGCACA CTGCACTTTC GGAGTTATGC TTCTCCATAA ATTATGTTCC AAACATGGCG 
GTGTGGGAGC ACACCTTCAC GCCGAGGGAG TATTTGACTT CTCATCTGGA AATCCGGTTC 
ACTAAATCAA TTGTTGGAAT GACTATGTAT AATCAAGCTA CACAGGAAAT TGCAAAGCCA 
TCAGAGCTTC TAACAAGCGT AAGAGCATAT ATGACTGTAC TCCAGTCCAT AGAGAACTAT 
GTGCAGATTG ATATCACCAG AGTATTTAAT AATGTCCTTC TTCAGCAAAC ACAACACTTA 
GACAGCCATG GAGAACCAAC CATCACGAGT CTGTATACAA ACTGGTACTT GGAAACTTTA 
TTAAGACAAG TCAGCAATGG CCATATAGCT TATTTTCCTG CAATGAAAGC ATTTGTGAAT 
TTACCCACAG AAAATGAATT AACATTCAAT GCAGAGGAAT ATTCTGATAT ATCAGAAATG 
AGATCATTAT CAGAGCTCCT AGGCCCATAT GGGATGAAGT TCCTGAGTGA GAGCCTTATG 
TGGCACATTT CATCACAAGT AGCTGAACTT AAGAAACTTG TGGTGGAGAA TGTTGATGTT 
CTAACACAAA TGAGGACCAG CTTTGACAAA CCAGACCAGA TGGCTGCACT CTTTAAAAGA 
TTATCATCTG TTGACAGTGT CTTGAAAAGG ATGACGATAA TTGGTGTTAT TTTATCCTTC 
CGGTCATTGG CACAAGAAGC ATTGAGAGAT GTCCTGTCCT ACCACATTCC TTTCCTCGTG 
AGCTCCATTG AAGATTTCAA GGATCACATT CCTCGGGAAA CTGATATGAA GGTTGCAATG 
AATGTGTATG AGCTGTCCTC GGCTGCTGGG TTACCATGTG AGATTGATCC TGCCTTAGTC 
GTAGCTCTTT CCTCACAAAA ATCAGAGAAC ATTAGTCCAG AGGAAGAATA CAAGATCGCT 
TGTCTCCTGA TGGTCTTTGT AGCAGTTTCC TTGCCTACAC TGGCCAGCAA TGTAATGTCA 
CAGTACAGCC CTGCAATAGA AGGACACTGC AACAATATAC ATTGTTTGGC CAAAGCCATC 
AACCAGATTG CTGCAGCTTT GTTTACAATT CACAAAGGAA GTATTGAAGA CCGACTTAAG 
GAATTCCTGG CGCTTGCATC ATCCAGTCTA CTGAAAATTG GCCAAGAGAC AGATAAAACT 
ACAACAAGAA ATAGAGAATC TGTTTATTTG CTACTGGATA TGATTGTACA AGAATCACCA 
TTCCTGACAA TGGATCTCTT GGAATCTTGT TTTCCTTATG TGTTGCTGAG AAATGCATAC 
CATGCTGTCT ACAAACAAAG TGTTACATCT TCTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap1 ( NM_001355411.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001342340.1 Mus musculus NCK-associated protein 1 (Nckap1), transcript variant 4, mRNA.

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CloneID	OMu30691
Clone ID Related Accession (Same CDS sequence)	XM_006499882.2 , NM_001355411.1
Accession Version	NM_001355411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	nck-associated protein 1 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL928578.6. On Sep 20, 2017 this sequence version replaced XM_006499882.2. Transcript Variant: This variant (4) lacks an alternate in-frame exon and uses an alternate in-frame splice junction in the 5' end compared to variant 1, resulting in an isoform (d) that is shorter compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.257896.1, SRR1660821.43876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACGCGC CTCTACAACA TCAAGAAGGC ATGTGGCGAC 
CCCAAGGCTA AACCCTCCTA CCTTATTGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TTCCTGCTGT AGAAACGCGC AACAACAATC TGGCACAGCT ACAGAAAGAA 
AAATCAGAGA TTCTGAAAAA TCTGGCGTTG TATTACTTTA CATTTGTAGA TGTTATGGAA 
TTTAAGGACC ATGTCTGTGA CTTGCTGAAT ACTATTGACG TTTGCCAAGT CTTCTTTGAT 
ATTACTGTGA ACTTTGATTT AACAAAGAAC TACTTAGACT TGACTGTAAC CTATACAACA 
TTAATGATAC TGCTGTCCCG GATTGAAGAA CGGAAGGCAA TCATTGGACT ATACAATTAT 
GCACATGAAA TGACCCATGG AGCAAGTGAC CGAGAATATC CACGCCTTGG TCAGATGATT 
GTGGACTATG AAAACCCTTT AAAAAAGATG ATGGAAGAAT TTGTACCCCA TAGCAAGTCT 
CTTTCAGATG CGCTAATTTC TCTTCAGATG GTGTATCCTA GACGGAACCT TTCAGCAGAC 
CAGTGGAGGA ATGCCCAGTT GCTGAGTCTC ATCAGTGCAC CCAGCACAAT GCTTAATCCT 
GCGCAGTCGG ACACTATGCC TTGTGAATAT CTCTCCTTGG ATGCAATGGA GAAATGGATC 
ATCTTTGGCT TTATTCTGTG CCATGGGATG CTAAATACGG AGGCTACCGC ACTGAACCTT 
TGGAAGCTAG CTCTTCAGAG TAGCTCTTGC CTCTCTCTCT TTCGGGATGA AGTTTTCCAC 
ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATTCGGG GCTACAATAA ACGTATTAAC 
GACATAAGAG AATGCAAGGA GGCGGCTGTA TCCCACGCCG GCTCAATGCA CAGAGAAAGA 
CGCAAGTTTT TACGGTCTGC ACTAAAAGAA CTGGCCACTG TCCTCTCTGA CCAGCCTGGC 
CTGCTTGGTC CCAAGGCACT TTTTGTTTTC ATGGCGTTAT CTTTTGCCCG TGATGAAATT 
ATCTGGCTGC TCCGTCACGC GGACAACATG CCAAAGAAGA GTGCAGATGA CTTTATAGAT 
AAGCACATTG CTGAGCTAAT ATTTTACATG GAAGAGCTTA GAGCACATGT CAGGAAGTAT 
GGGCCTGTCA TGCAGAGGTA CTACGTGCAA TACCTCTCTG GCTTTGATGC TGTTGTCCTC 
AATGAACTTG TGCAGAACCT GTCTGTTTGC CCTGAGGATG AATCGATCAT CATGTCCTCT 
TTTGTGAACA CCATGACCTC CCTAAGTGTG AAGCAAGTTG AAGATGGAGA AGTGTTTGAT 
TTCAGAGGGA TGAGATTAGA TTGGTTTAGG TTACAGGCGT ACACTAGCGT CTCCAAAGCT 
TCACTTAGCC TTGCAGATCA CAGAGAACTT GGAAAGATGA TGAACACAAT CATATTCCAT 
ACAAAAATGG TAGATTCCTT GGTTGAAATG TTGGTGGAAA CATCCGATCT CTCCATATTT 
TGTTTTTACA GCCGTGCTTT TGAGAAGATG TTTCAGCAGT GCTTGGAGCT GCCCTCTCAG 
TCAAGATACT CCATTGCCTT TCCTCTGCTC TGCACTCACT TCATGAGCTG CACACATGAA 
CTGTGTCCTG AAGAGCGCCA TCATATAGGA GATCGTAGCC TTTCCCTGTG CAATATGTTC 
CTGGATGAGA TGGCCAAACA AGCTCGAAAT CTCATCACCG ATATTTGCAC AGAACAGTGT 
ACTCTTAGTG ACCAGTTATT GCCGAAGCAT TGTGCCAAAA CCATCAGTCA AGCAGTGAAT 
AAGAAGTCAA AAAAACAGAC GGGCAAGAAA GGGGAACCTG AAAGGGAAAA ACCGGGTGTG 
GAGAGCATGA GGAAAAACAG GCTGGTAGTG ACCAACCTTG ATAAGTTGCA CACTGCACTT 
TCGGAGTTAT GCTTCTCCAT AAATTATGTT CCAAACATGG CGGTGTGGGA GCACACCTTC 
ACGCCGAGGG AGTATTTGAC TTCTCATCTG GAAATCCGGT TCACTAAATC AATTGTTGGA 
ATGACTATGT ATAATCAAGC TACACAGGAA ATTGCAAAGC CATCAGAGCT TCTAACAAGC 
GTAAGAGCAT ATATGACTGT ACTCCAGTCC ATAGAGAACT ATGTGCAGAT TGATATCACC 
AGAGTATTTA ATAATGTCCT TCTTCAGCAA ACACAACACT TAGACAGCCA TGGAGAACCA 
ACCATCACGA GTCTGTATAC AAACTGGTAC TTGGAAACTT TATTAAGACA AGTCAGCAAT 
GGCCATATAG CTTATTTTCC TGCAATGAAA GCATTTGTGA ATTTACCCAC AGAAAATGAA 
TTAACATTCA ATGCAGAGGA ATATTCTGAT ATATCAGAAA TGAGATCATT ATCAGAGCTC 
CTAGGCCCAT ATGGGATGAA GTTCCTGAGT GAGAGCCTTA TGTGGCACAT TTCATCACAA 
GTAGCTGAAC TTAAGAAACT TGTGGTGGAG AATGTTGATG TTCTAACACA AATGAGGACC 
AGCTTTGACA AACCAGACCA GATGGCTGCA CTCTTTAAAA GATTATCATC TGTTGACAGT 
GTCTTGAAAA GGATGACGAT AATTGGTGTT ATTTTATCCT TCCGGTCATT GGCACAAGAA 
GCATTGAGAG ATGTCCTGTC CTACCACATT CCTTTCCTCG TGAGCTCCAT TGAAGATTTC 
AAGGATCACA TTCCTCGGGA AACTGATATG AAGGTTGCAA TGAATGTGTA TGAGCTGTCC 
TCGGCTGCTG GGTTACCATG TGAGATTGAT CCTGCCTTAG TCGTAGCTCT TTCCTCACAA 
AAATCAGAGA ACATTAGTCC AGAGGAAGAA TACAAGATCG CTTGTCTCCT GATGGTCTTT 
GTAGCAGTTT CCTTGCCTAC ACTGGCCAGC AATGTAATGT CACAGTACAG CCCTGCAATA 
GAAGGACACT GCAACAATAT ACATTGTTTG GCCAAAGCCA TCAACCAGAT TGCTGCAGCT 
TTGTTTACAA TTCACAAAGG AAGTATTGAA GACCGACTTA AGGAATTCCT GGCGCTTGCA 
TCATCCAGTC TACTGAAAAT TGGCCAAGAG ACAGATAAAA CTACAACAAG AAATAGAGAA 
TCTGTTTATT TGCTACTGGA TATGATTGTA CAAGAATCAC CATTCCTGAC AATGGATCTC 
TTGGAATCTT GTTTTCCTTA TGTGTTGCTG AGAAATGCAT ACCATGCTGT CTACAAACAA 
AGTGTTACAT CTTCTGCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342340.1
CDS	218..3598
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCDLLNTIDVCQVFFDITVNFDLTKNYLDLTVTYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGMLNTEATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMAVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	NM_001355411.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK-associated protein 1 (Nckap1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355411.1

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACGCGC CTCTACAACA TCAAGAAGGC ATGTGGCGAC 
CCCAAGGCTA AACCCTCCTA CCTTATTGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TTCCTGCTGT AGAAACGCGC AACAACAATC TGGCACAGCT ACAGAAAGAA 
AAATCAGAGA TTCTGAAAAA TCTGGCGTTG TATTACTTTA CATTTGTAGA TGTTATGGAA 
TTTAAGGACC ATGTCTGTGA CTTGCTGAAT ACTATTGACG TTTGCCAAGT CTTCTTTGAT 
ATTACTGTGA ACTTTGATTT AACAAAGAAC TACTTAGACT TGACTGTAAC CTATACAACA 
TTAATGATAC TGCTGTCCCG GATTGAAGAA CGGAAGGCAA TCATTGGACT ATACAATTAT 
GCACATGAAA TGACCCATGG AGCAAGTGAC CGAGAATATC CACGCCTTGG TCAGATGATT 
GTGGACTATG AAAACCCTTT AAAAAAGATG ATGGAAGAAT TTGTACCCCA TAGCAAGTCT 
CTTTCAGATG CGCTAATTTC TCTTCAGATG GTGTATCCTA GACGGAACCT TTCAGCAGAC 
CAGTGGAGGA ATGCCCAGTT GCTGAGTCTC ATCAGTGCAC CCAGCACAAT GCTTAATCCT 
GCGCAGTCGG ACACTATGCC TTGTGAATAT CTCTCCTTGG ATGCAATGGA GAAATGGATC 
ATCTTTGGCT TTATTCTGTG CCATGGGATG CTAAATACGG AGGCTACCGC ACTGAACCTT 
TGGAAGCTAG CTCTTCAGAG TAGCTCTTGC CTCTCTCTCT TTCGGGATGA AGTTTTCCAC 
ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATTCGGG GCTACAATAA ACGTATTAAC 
GACATAAGAG AATGCAAGGA GGCGGCTGTA TCCCACGCCG GCTCAATGCA CAGAGAAAGA 
CGCAAGTTTT TACGGTCTGC ACTAAAAGAA CTGGCCACTG TCCTCTCTGA CCAGCCTGGC 
CTGCTTGGTC CCAAGGCACT TTTTGTTTTC ATGGCGTTAT CTTTTGCCCG TGATGAAATT 
ATCTGGCTGC TCCGTCACGC GGACAACATG CCAAAGAAGA GTGCAGATGA CTTTATAGAT 
AAGCACATTG CTGAGCTAAT ATTTTACATG GAAGAGCTTA GAGCACATGT CAGGAAGTAT 
GGGCCTGTCA TGCAGAGGTA CTACGTGCAA TACCTCTCTG GCTTTGATGC TGTTGTCCTC 
AATGAACTTG TGCAGAACCT GTCTGTTTGC CCTGAGGATG AATCGATCAT CATGTCCTCT 
TTTGTGAACA CCATGACCTC CCTAAGTGTG AAGCAAGTTG AAGATGGAGA AGTGTTTGAT 
TTCAGAGGGA TGAGATTAGA TTGGTTTAGG TTACAGGCGT ACACTAGCGT CTCCAAAGCT 
TCACTTAGCC TTGCAGATCA CAGAGAACTT GGAAAGATGA TGAACACAAT CATATTCCAT 
ACAAAAATGG TAGATTCCTT GGTTGAAATG TTGGTGGAAA CATCCGATCT CTCCATATTT 
TGTTTTTACA GCCGTGCTTT TGAGAAGATG TTTCAGCAGT GCTTGGAGCT GCCCTCTCAG 
TCAAGATACT CCATTGCCTT TCCTCTGCTC TGCACTCACT TCATGAGCTG CACACATGAA 
CTGTGTCCTG AAGAGCGCCA TCATATAGGA GATCGTAGCC TTTCCCTGTG CAATATGTTC 
CTGGATGAGA TGGCCAAACA AGCTCGAAAT CTCATCACCG ATATTTGCAC AGAACAGTGT 
ACTCTTAGTG ACCAGTTATT GCCGAAGCAT TGTGCCAAAA CCATCAGTCA AGCAGTGAAT 
AAGAAGTCAA AAAAACAGAC GGGCAAGAAA GGGGAACCTG AAAGGGAAAA ACCGGGTGTG 
GAGAGCATGA GGAAAAACAG GCTGGTAGTG ACCAACCTTG ATAAGTTGCA CACTGCACTT 
TCGGAGTTAT GCTTCTCCAT AAATTATGTT CCAAACATGG CGGTGTGGGA GCACACCTTC 
ACGCCGAGGG AGTATTTGAC TTCTCATCTG GAAATCCGGT TCACTAAATC AATTGTTGGA 
ATGACTATGT ATAATCAAGC TACACAGGAA ATTGCAAAGC CATCAGAGCT TCTAACAAGC 
GTAAGAGCAT ATATGACTGT ACTCCAGTCC ATAGAGAACT ATGTGCAGAT TGATATCACC 
AGAGTATTTA ATAATGTCCT TCTTCAGCAA ACACAACACT TAGACAGCCA TGGAGAACCA 
ACCATCACGA GTCTGTATAC AAACTGGTAC TTGGAAACTT TATTAAGACA AGTCAGCAAT 
GGCCATATAG CTTATTTTCC TGCAATGAAA GCATTTGTGA ATTTACCCAC AGAAAATGAA 
TTAACATTCA ATGCAGAGGA ATATTCTGAT ATATCAGAAA TGAGATCATT ATCAGAGCTC 
CTAGGCCCAT ATGGGATGAA GTTCCTGAGT GAGAGCCTTA TGTGGCACAT TTCATCACAA 
GTAGCTGAAC TTAAGAAACT TGTGGTGGAG AATGTTGATG TTCTAACACA AATGAGGACC 
AGCTTTGACA AACCAGACCA GATGGCTGCA CTCTTTAAAA GATTATCATC TGTTGACAGT 
GTCTTGAAAA GGATGACGAT AATTGGTGTT ATTTTATCCT TCCGGTCATT GGCACAAGAA 
GCATTGAGAG ATGTCCTGTC CTACCACATT CCTTTCCTCG TGAGCTCCAT TGAAGATTTC 
AAGGATCACA TTCCTCGGGA AACTGATATG AAGGTTGCAA TGAATGTGTA TGAGCTGTCC 
TCGGCTGCTG GGTTACCATG TGAGATTGAT CCTGCCTTAG TCGTAGCTCT TTCCTCACAA 
AAATCAGAGA ACATTAGTCC AGAGGAAGAA TACAAGATCG CTTGTCTCCT GATGGTCTTT 
GTAGCAGTTT CCTTGCCTAC ACTGGCCAGC AATGTAATGT CACAGTACAG CCCTGCAATA 
GAAGGACACT GCAACAATAT ACATTGTTTG GCCAAAGCCA TCAACCAGAT TGCTGCAGCT 
TTGTTTACAA TTCACAAAGG AAGTATTGAA GACCGACTTA AGGAATTCCT GGCGCTTGCA 
TCATCCAGTC TACTGAAAAT TGGCCAAGAG ACAGATAAAA CTACAACAAG AAATAGAGAA 
TCTGTTTATT TGCTACTGGA TATGATTGTA CAAGAATCAC CATTCCTGAC AATGGATCTC 
TTGGAATCTT GTTTTCCTTA TGTGTTGCTG AGAAATGCAT ACCATGCTGT CTACAAACAA 
AGTGTTACAT CTTCTGCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap1 ( XM_006499881.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499944.1 Mus musculus NCK-associated protein 1 (Nckap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30690
Clone ID Related Accession (Same CDS sequence)	NM_001355410.1 , XM_006499881.3
Accession Version	XM_006499881.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	nck-associated protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499881.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACGCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGCGACCC CAAGGCTAAA CCCTCCTACC TTATTGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTT CCTGCTGTAG AAACGCGCAA CAACAATCTG 
GCACAGCTAC AGAAAGAAAA ATCAGAGATT CTGAAAAATC TGGCGTTGTA TTACTTTACA 
TTTGTAGATG TTATGGAATT TAAGGACCAT GTCTGTGACT TGCTGAATAC TATTGACGTT 
TGCCAAGTCT TCTTTGATAT TACTGTGAAC TTTGATTTAA CAAAGAACTA CTTAGACTTG 
ACTGTAACCT ATACAACATT AATGATACTG CTGTCCCGGA TTGAAGAACG GAAGGCAATC 
ATTGGACTAT ACAATTATGC ACATGAAATG ACCCATGGAG CAAGTGACCG AGAATATCCA 
CGCCTTGGTC AGATGATTGT GGACTATGAA AACCCTTTAA AAAAGATGAT GGAAGAATTT 
GTACCCCATA GCAAGTCTCT TTCAGATGCG CTAATTTCTC TTCAGATGGT GTATCCTAGA 
CGGAACCTTT CAGCAGACCA GTGGAGGAAT GCCCAGTTGC TGAGTCTCAT CAGTGCACCC 
AGCACAATGC TTAATCCTGC GCAGTCGGAC ACTATGCCTT GTGAATATCT CTCCTTGGAT 
GCAATGGAGA AATGGATCAT CTTTGGCTTT ATTCTGTGCC ATGGGATGCT AAATACGGAG 
GCTACCGCAC TGAACCTTTG GAAGCTAGCT CTTCAGAGTA GCTCTTGCCT CTCTCTCTTT 
CGGGATGAAG TTTTCCACAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATTCGGGGC 
TACAATAAAC GTATTAACGA CATAAGAGAA TGCAAGGAGG CGGCTGTATC CCACGCCGGC 
TCAATGCACA GAGAAAGACG CAAGTTTTTA CGGTCTGCAC TAAAAGAACT GGCCACTGTC 
CTCTCTGACC AGCCTGGCCT GCTTGGTCCC AAGGCACTTT TTGTTTTCAT GGCGTTATCT 
TTTGCCCGTG ATGAAATTAT CTGGCTGCTC CGTCACGCGG ACAACATGCC AAAGAAGAGT 
GCAGATGACT TTATAGATAA GCACATTGCT GAGCTAATAT TTTACATGGA AGAGCTTAGA 
GCACATGTCA GGAAGTATGG GCCTGTCATG CAGAGGTACT ACGTGCAATA CCTCTCTGGC 
TTTGATGCTG TTGTCCTCAA TGAACTTGTG CAGAACCTGT CTGTTTGCCC TGAGGATGAA 
TCGATCATCA TGTCCTCTTT TGTGAACACC ATGACCTCCC TAAGTGTGAA GCAAGTTGAA 
GATGGAGAAG TGTTTGATTT CAGAGGGATG AGATTAGATT GGTTTAGGTT ACAGGCGTAC 
ACTAGCGTCT CCAAAGCTTC ACTTAGCCTT GCAGATCACA GAGAACTTGG AAAGATGATG 
AACACAATCA TATTCCATAC AAAAATGGTA GATTCCTTGG TTGAAATGTT GGTGGAAACA 
TCCGATCTCT CCATATTTTG TTTTTACAGC CGTGCTTTTG AGAAGATGTT TCAGCAGTGC 
TTGGAGCTGC CCTCTCAGTC AAGATACTCC ATTGCCTTTC CTCTGCTCTG CACTCACTTC 
ATGAGCTGCA CACATGAACT GTGTCCTGAA GAGCGCCATC ATATAGGAGA TCGTAGCCTT 
TCCCTGTGCA ATATGTTCCT GGATGAGATG GCCAAACAAG CTCGAAATCT CATCACCGAT 
ATTTGCACAG AACAGTGTAC TCTTAGTGAC CAGTTATTGC CGAAGCATTG TGCCAAAACC 
ATCAGTCAAG CAGTGAATAA GAAGTCAAAA AAACAGACGG GCAAGAAAGG GGAACCTGAA 
AGGGAAAAAC CGGGTGTGGA GAGCATGAGG AAAAACAGGC TGGTAGTGAC CAACCTTGAT 
AAGTTGCACA CTGCACTTTC GGAGTTATGC TTCTCCATAA ATTATGTTCC AAACATGGCG 
GTGTGGGAGC ACACCTTCAC GCCGAGGGAG TATTTGACTT CTCATCTGGA AATCCGGTTC 
ACTAAATCAA TTGTTGGAAT GACTATGTAT AATCAAGCTA CACAGGAAAT TGCAAAGCCA 
TCAGAGCTTC TAACAAGCGT AAGAGCATAT ATGACTGTAC TCCAGTCCAT AGAGAACTAT 
GTGCAGATTG ATATCACCAG AGTATTTAAT AATGTCCTTC TTCAGCAAAC ACAACACTTA 
GACAGCCATG GAGAACCAAC CATCACGAGT CTGTATACAA ACTGGTACTT GGAAACTTTA 
TTAAGACAAG TCAGCAATGG CCATATAGCT TATTTTCCTG CAATGAAAGC ATTTGTGAAT 
TTACCCACAG AAAATGAATT AACATTCAAT GCAGAGGAAT ATTCTGATAT ATCAGAAATG 
AGATCATTAT CAGAGCTCCT AGGCCCATAT GGGATGAAGT TCCTGAGTGA GAGCCTTATG 
TGGCACATTT CATCACAAGT AGCTGAACTT AAGAAACTTG TGGTGGAGAA TGTTGATGTT 
CTAACACAAA TGAGGACCAG CTTTGACAAA CCAGACCAGA TGGCTGCACT CTTTAAAAGA 
TTATCATCTG TTGACAGTGT CTTGAAAAGG ATGACGATAA TTGGTGTTAT TTTATCCTTC 
CGGTCATTGG CACAAGAAGC ATTGAGAGAT GTCCTGTCCT ACCACATTCC TTTCCTCGTG 
AGCTCCATTG AAGATTTCAA GGATCACATT CCTCGGGAAA CTGATATGAA GGTTGCAATG 
AATGTGTATG AGCTGTCCTC GGCTGCTGGG TTACCATGTG AGATTGATCC TGCCTTAGTC 
GTAGCTCTTT CCTCACAAAA ATCAGAGAAC ATTAGTCCAG AGGAAGAATA CAAGATCGCT 
TGTCTCCTGA TGGTCTTTGT AGCAGTTTCC TTGCCTACAC TGGCCAGCAA TGTAATGTCA 
CAGTACAGCC CTGCAATAGA AGGACACTGC AACAATATAC ATTGTTTGGC CAAAGCCATC 
AACCAGATTG CTGCAGCTTT GTTTACAATT CACAAAGGAA GTATTGAAGA CCGACTTAAG 
GAATTCCTGG CGCTTGCATC ATCCAGTCTA CTGAAAATTG GCCAAGAGAC AGATAAAACT 
ACAACAAGAA ATAGAGAATC TGTTTATTTG CTACTGGATA TGATTGTACA AGAATCACCA 
TTCCTGACAA TGGATCTCTT GGAATCTTGT TTTCCTTATG TGTTGCTGAG AAATGCATAC 
CATGCTGTCT ACAAACAAAG TGTTACATCT TCTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499944.1
CDS	22..3420
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCDLLNTIDVCQVFFDITVNFDLTKNYLDLTVTYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGMLNTEATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMAVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_006499881.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus NCK-associated protein 1 (Nckap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499881.3

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACGCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGCGACCC CAAGGCTAAA CCCTCCTACC TTATTGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTT CCTGCTGTAG AAACGCGCAA CAACAATCTG 
GCACAGCTAC AGAAAGAAAA ATCAGAGATT CTGAAAAATC TGGCGTTGTA TTACTTTACA 
TTTGTAGATG TTATGGAATT TAAGGACCAT GTCTGTGACT TGCTGAATAC TATTGACGTT 
TGCCAAGTCT TCTTTGATAT TACTGTGAAC TTTGATTTAA CAAAGAACTA CTTAGACTTG 
ACTGTAACCT ATACAACATT AATGATACTG CTGTCCCGGA TTGAAGAACG GAAGGCAATC 
ATTGGACTAT ACAATTATGC ACATGAAATG ACCCATGGAG CAAGTGACCG AGAATATCCA 
CGCCTTGGTC AGATGATTGT GGACTATGAA AACCCTTTAA AAAAGATGAT GGAAGAATTT 
GTACCCCATA GCAAGTCTCT TTCAGATGCG CTAATTTCTC TTCAGATGGT GTATCCTAGA 
CGGAACCTTT CAGCAGACCA GTGGAGGAAT GCCCAGTTGC TGAGTCTCAT CAGTGCACCC 
AGCACAATGC TTAATCCTGC GCAGTCGGAC ACTATGCCTT GTGAATATCT CTCCTTGGAT 
GCAATGGAGA AATGGATCAT CTTTGGCTTT ATTCTGTGCC ATGGGATGCT AAATACGGAG 
GCTACCGCAC TGAACCTTTG GAAGCTAGCT CTTCAGAGTA GCTCTTGCCT CTCTCTCTTT 
CGGGATGAAG TTTTCCACAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATTCGGGGC 
TACAATAAAC GTATTAACGA CATAAGAGAA TGCAAGGAGG CGGCTGTATC CCACGCCGGC 
TCAATGCACA GAGAAAGACG CAAGTTTTTA CGGTCTGCAC TAAAAGAACT GGCCACTGTC 
CTCTCTGACC AGCCTGGCCT GCTTGGTCCC AAGGCACTTT TTGTTTTCAT GGCGTTATCT 
TTTGCCCGTG ATGAAATTAT CTGGCTGCTC CGTCACGCGG ACAACATGCC AAAGAAGAGT 
GCAGATGACT TTATAGATAA GCACATTGCT GAGCTAATAT TTTACATGGA AGAGCTTAGA 
GCACATGTCA GGAAGTATGG GCCTGTCATG CAGAGGTACT ACGTGCAATA CCTCTCTGGC 
TTTGATGCTG TTGTCCTCAA TGAACTTGTG CAGAACCTGT CTGTTTGCCC TGAGGATGAA 
TCGATCATCA TGTCCTCTTT TGTGAACACC ATGACCTCCC TAAGTGTGAA GCAAGTTGAA 
GATGGAGAAG TGTTTGATTT CAGAGGGATG AGATTAGATT GGTTTAGGTT ACAGGCGTAC 
ACTAGCGTCT CCAAAGCTTC ACTTAGCCTT GCAGATCACA GAGAACTTGG AAAGATGATG 
AACACAATCA TATTCCATAC AAAAATGGTA GATTCCTTGG TTGAAATGTT GGTGGAAACA 
TCCGATCTCT CCATATTTTG TTTTTACAGC CGTGCTTTTG AGAAGATGTT TCAGCAGTGC 
TTGGAGCTGC CCTCTCAGTC AAGATACTCC ATTGCCTTTC CTCTGCTCTG CACTCACTTC 
ATGAGCTGCA CACATGAACT GTGTCCTGAA GAGCGCCATC ATATAGGAGA TCGTAGCCTT 
TCCCTGTGCA ATATGTTCCT GGATGAGATG GCCAAACAAG CTCGAAATCT CATCACCGAT 
ATTTGCACAG AACAGTGTAC TCTTAGTGAC CAGTTATTGC CGAAGCATTG TGCCAAAACC 
ATCAGTCAAG CAGTGAATAA GAAGTCAAAA AAACAGACGG GCAAGAAAGG GGAACCTGAA 
AGGGAAAAAC CGGGTGTGGA GAGCATGAGG AAAAACAGGC TGGTAGTGAC CAACCTTGAT 
AAGTTGCACA CTGCACTTTC GGAGTTATGC TTCTCCATAA ATTATGTTCC AAACATGGCG 
GTGTGGGAGC ACACCTTCAC GCCGAGGGAG TATTTGACTT CTCATCTGGA AATCCGGTTC 
ACTAAATCAA TTGTTGGAAT GACTATGTAT AATCAAGCTA CACAGGAAAT TGCAAAGCCA 
TCAGAGCTTC TAACAAGCGT AAGAGCATAT ATGACTGTAC TCCAGTCCAT AGAGAACTAT 
GTGCAGATTG ATATCACCAG AGTATTTAAT AATGTCCTTC TTCAGCAAAC ACAACACTTA 
GACAGCCATG GAGAACCAAC CATCACGAGT CTGTATACAA ACTGGTACTT GGAAACTTTA 
TTAAGACAAG TCAGCAATGG CCATATAGCT TATTTTCCTG CAATGAAAGC ATTTGTGAAT 
TTACCCACAG AAAATGAATT AACATTCAAT GCAGAGGAAT ATTCTGATAT ATCAGAAATG 
AGATCATTAT CAGAGCTCCT AGGCCCATAT GGGATGAAGT TCCTGAGTGA GAGCCTTATG 
TGGCACATTT CATCACAAGT AGCTGAACTT AAGAAACTTG TGGTGGAGAA TGTTGATGTT 
CTAACACAAA TGAGGACCAG CTTTGACAAA CCAGACCAGA TGGCTGCACT CTTTAAAAGA 
TTATCATCTG TTGACAGTGT CTTGAAAAGG ATGACGATAA TTGGTGTTAT TTTATCCTTC 
CGGTCATTGG CACAAGAAGC ATTGAGAGAT GTCCTGTCCT ACCACATTCC TTTCCTCGTG 
AGCTCCATTG AAGATTTCAA GGATCACATT CCTCGGGAAA CTGATATGAA GGTTGCAATG 
AATGTGTATG AGCTGTCCTC GGCTGCTGGG TTACCATGTG AGATTGATCC TGCCTTAGTC 
GTAGCTCTTT CCTCACAAAA ATCAGAGAAC ATTAGTCCAG AGGAAGAATA CAAGATCGCT 
TGTCTCCTGA TGGTCTTTGT AGCAGTTTCC TTGCCTACAC TGGCCAGCAA TGTAATGTCA 
CAGTACAGCC CTGCAATAGA AGGACACTGC AACAATATAC ATTGTTTGGC CAAAGCCATC 
AACCAGATTG CTGCAGCTTT GTTTACAATT CACAAAGGAA GTATTGAAGA CCGACTTAAG 
GAATTCCTGG CGCTTGCATC ATCCAGTCTA CTGAAAATTG GCCAAGAGAC AGATAAAACT 
ACAACAAGAA ATAGAGAATC TGTTTATTTG CTACTGGATA TGATTGTACA AGAATCACCA 
TTCCTGACAA TGGATCTCTT GGAATCTTGT TTTCCTTATG TGTTGCTGAG AAATGCATAC 
CATGCTGTCT ACAAACAAAG TGTTACATCT TCTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap1 ( XM_006499882.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499945.1 Mus musculus NCK-associated protein 1 (Nckap1), transcript variant X2, mRNA.

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CloneID	OMu30691
Clone ID Related Accession (Same CDS sequence)	XM_006499882.2 , NM_001355411.1
Accession Version	XM_006499882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	nck-associated protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACGCGC CTCTACAACA TCAAGAAGGC ATGTGGCGAC 
CCCAAGGCTA AACCCTCCTA CCTTATTGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TTCCTGCTGT AGAAACGCGC AACAACAATC TGGCACAGCT ACAGAAAGAA 
AAATCAGAGA TTCTGAAAAA TCTGGCGTTG TATTACTTTA CATTTGTAGA TGTTATGGAA 
TTTAAGGACC ATGTCTGTGA CTTGCTGAAT ACTATTGACG TTTGCCAAGT CTTCTTTGAT 
ATTACTGTGA ACTTTGATTT AACAAAGAAC TACTTAGACT TGACTGTAAC CTATACAACA 
TTAATGATAC TGCTGTCCCG GATTGAAGAA CGGAAGGCAA TCATTGGACT ATACAATTAT 
GCACATGAAA TGACCCATGG AGCAAGTGAC CGAGAATATC CACGCCTTGG TCAGATGATT 
GTGGACTATG AAAACCCTTT AAAAAAGATG ATGGAAGAAT TTGTACCCCA TAGCAAGTCT 
CTTTCAGATG CGCTAATTTC TCTTCAGATG GTGTATCCTA GACGGAACCT TTCAGCAGAC 
CAGTGGAGGA ATGCCCAGTT GCTGAGTCTC ATCAGTGCAC CCAGCACAAT GCTTAATCCT 
GCGCAGTCGG ACACTATGCC TTGTGAATAT CTCTCCTTGG ATGCAATGGA GAAATGGATC 
ATCTTTGGCT TTATTCTGTG CCATGGGATG CTAAATACGG AGGCTACCGC ACTGAACCTT 
TGGAAGCTAG CTCTTCAGAG TAGCTCTTGC CTCTCTCTCT TTCGGGATGA AGTTTTCCAC 
ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATTCGGG GCTACAATAA ACGTATTAAC 
GACATAAGAG AATGCAAGGA GGCGGCTGTA TCCCACGCCG GCTCAATGCA CAGAGAAAGA 
CGCAAGTTTT TACGGTCTGC ACTAAAAGAA CTGGCCACTG TCCTCTCTGA CCAGCCTGGC 
CTGCTTGGTC CCAAGGCACT TTTTGTTTTC ATGGCGTTAT CTTTTGCCCG TGATGAAATT 
ATCTGGCTGC TCCGTCACGC GGACAACATG CCAAAGAAGA GTGCAGATGA CTTTATAGAT 
AAGCACATTG CTGAGCTAAT ATTTTACATG GAAGAGCTTA GAGCACATGT CAGGAAGTAT 
GGGCCTGTCA TGCAGAGGTA CTACGTGCAA TACCTCTCTG GCTTTGATGC TGTTGTCCTC 
AATGAACTTG TGCAGAACCT GTCTGTTTGC CCTGAGGATG AATCGATCAT CATGTCCTCT 
TTTGTGAACA CCATGACCTC CCTAAGTGTG AAGCAAGTTG AAGATGGAGA AGTGTTTGAT 
TTCAGAGGGA TGAGATTAGA TTGGTTTAGG TTACAGGCGT ACACTAGCGT CTCCAAAGCT 
TCACTTAGCC TTGCAGATCA CAGAGAACTT GGAAAGATGA TGAACACAAT CATATTCCAT 
ACAAAAATGG TAGATTCCTT GGTTGAAATG TTGGTGGAAA CATCCGATCT CTCCATATTT 
TGTTTTTACA GCCGTGCTTT TGAGAAGATG TTTCAGCAGT GCTTGGAGCT GCCCTCTCAG 
TCAAGATACT CCATTGCCTT TCCTCTGCTC TGCACTCACT TCATGAGCTG CACACATGAA 
CTGTGTCCTG AAGAGCGCCA TCATATAGGA GATCGTAGCC TTTCCCTGTG CAATATGTTC 
CTGGATGAGA TGGCCAAACA AGCTCGAAAT CTCATCACCG ATATTTGCAC AGAACAGTGT 
ACTCTTAGTG ACCAGTTATT GCCGAAGCAT TGTGCCAAAA CCATCAGTCA AGCAGTGAAT 
AAGAAGTCAA AAAAACAGAC GGGCAAGAAA GGGGAACCTG AAAGGGAAAA ACCGGGTGTG 
GAGAGCATGA GGAAAAACAG GCTGGTAGTG ACCAACCTTG ATAAGTTGCA CACTGCACTT 
TCGGAGTTAT GCTTCTCCAT AAATTATGTT CCAAACATGG CGGTGTGGGA GCACACCTTC 
ACGCCGAGGG AGTATTTGAC TTCTCATCTG GAAATCCGGT TCACTAAATC AATTGTTGGA 
ATGACTATGT ATAATCAAGC TACACAGGAA ATTGCAAAGC CATCAGAGCT TCTAACAAGC 
GTAAGAGCAT ATATGACTGT ACTCCAGTCC ATAGAGAACT ATGTGCAGAT TGATATCACC 
AGAGTATTTA ATAATGTCCT TCTTCAGCAA ACACAACACT TAGACAGCCA TGGAGAACCA 
ACCATCACGA GTCTGTATAC AAACTGGTAC TTGGAAACTT TATTAAGACA AGTCAGCAAT 
GGCCATATAG CTTATTTTCC TGCAATGAAA GCATTTGTGA ATTTACCCAC AGAAAATGAA 
TTAACATTCA ATGCAGAGGA ATATTCTGAT ATATCAGAAA TGAGATCATT ATCAGAGCTC 
CTAGGCCCAT ATGGGATGAA GTTCCTGAGT GAGAGCCTTA TGTGGCACAT TTCATCACAA 
GTAGCTGAAC TTAAGAAACT TGTGGTGGAG AATGTTGATG TTCTAACACA AATGAGGACC 
AGCTTTGACA AACCAGACCA GATGGCTGCA CTCTTTAAAA GATTATCATC TGTTGACAGT 
GTCTTGAAAA GGATGACGAT AATTGGTGTT ATTTTATCCT TCCGGTCATT GGCACAAGAA 
GCATTGAGAG ATGTCCTGTC CTACCACATT CCTTTCCTCG TGAGCTCCAT TGAAGATTTC 
AAGGATCACA TTCCTCGGGA AACTGATATG AAGGTTGCAA TGAATGTGTA TGAGCTGTCC 
TCGGCTGCTG GGTTACCATG TGAGATTGAT CCTGCCTTAG TCGTAGCTCT TTCCTCACAA 
AAATCAGAGA ACATTAGTCC AGAGGAAGAA TACAAGATCG CTTGTCTCCT GATGGTCTTT 
GTAGCAGTTT CCTTGCCTAC ACTGGCCAGC AATGTAATGT CACAGTACAG CCCTGCAATA 
GAAGGACACT GCAACAATAT ACATTGTTTG GCCAAAGCCA TCAACCAGAT TGCTGCAGCT 
TTGTTTACAA TTCACAAAGG AAGTATTGAA GACCGACTTA AGGAATTCCT GGCGCTTGCA 
TCATCCAGTC TACTGAAAAT TGGCCAAGAG ACAGATAAAA CTACAACAAG AAATAGAGAA 
TCTGTTTATT TGCTACTGGA TATGATTGTA CAAGAATCAC CATTCCTGAC AATGGATCTC 
TTGGAATCTT GTTTTCCTTA TGTGTTGCTG AGAAATGCAT ACCATGCTGT CTACAAACAA 
AGTGTTACAT CTTCTGCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499945.1
CDS	335..3715
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCDLLNTIDVCQVFFDITVNFDLTKNYLDLTVTYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGMLNTEATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMAVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_006499882.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus NCK-associated protein 1 (Nckap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499882.2

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACGCGC CTCTACAACA TCAAGAAGGC ATGTGGCGAC 
CCCAAGGCTA AACCCTCCTA CCTTATTGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TTCCTGCTGT AGAAACGCGC AACAACAATC TGGCACAGCT ACAGAAAGAA 
AAATCAGAGA TTCTGAAAAA TCTGGCGTTG TATTACTTTA CATTTGTAGA TGTTATGGAA 
TTTAAGGACC ATGTCTGTGA CTTGCTGAAT ACTATTGACG TTTGCCAAGT CTTCTTTGAT 
ATTACTGTGA ACTTTGATTT AACAAAGAAC TACTTAGACT TGACTGTAAC CTATACAACA 
TTAATGATAC TGCTGTCCCG GATTGAAGAA CGGAAGGCAA TCATTGGACT ATACAATTAT 
GCACATGAAA TGACCCATGG AGCAAGTGAC CGAGAATATC CACGCCTTGG TCAGATGATT 
GTGGACTATG AAAACCCTTT AAAAAAGATG ATGGAAGAAT TTGTACCCCA TAGCAAGTCT 
CTTTCAGATG CGCTAATTTC TCTTCAGATG GTGTATCCTA GACGGAACCT TTCAGCAGAC 
CAGTGGAGGA ATGCCCAGTT GCTGAGTCTC ATCAGTGCAC CCAGCACAAT GCTTAATCCT 
GCGCAGTCGG ACACTATGCC TTGTGAATAT CTCTCCTTGG ATGCAATGGA GAAATGGATC 
ATCTTTGGCT TTATTCTGTG CCATGGGATG CTAAATACGG AGGCTACCGC ACTGAACCTT 
TGGAAGCTAG CTCTTCAGAG TAGCTCTTGC CTCTCTCTCT TTCGGGATGA AGTTTTCCAC 
ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATTCGGG GCTACAATAA ACGTATTAAC 
GACATAAGAG AATGCAAGGA GGCGGCTGTA TCCCACGCCG GCTCAATGCA CAGAGAAAGA 
CGCAAGTTTT TACGGTCTGC ACTAAAAGAA CTGGCCACTG TCCTCTCTGA CCAGCCTGGC 
CTGCTTGGTC CCAAGGCACT TTTTGTTTTC ATGGCGTTAT CTTTTGCCCG TGATGAAATT 
ATCTGGCTGC TCCGTCACGC GGACAACATG CCAAAGAAGA GTGCAGATGA CTTTATAGAT 
AAGCACATTG CTGAGCTAAT ATTTTACATG GAAGAGCTTA GAGCACATGT CAGGAAGTAT 
GGGCCTGTCA TGCAGAGGTA CTACGTGCAA TACCTCTCTG GCTTTGATGC TGTTGTCCTC 
AATGAACTTG TGCAGAACCT GTCTGTTTGC CCTGAGGATG AATCGATCAT CATGTCCTCT 
TTTGTGAACA CCATGACCTC CCTAAGTGTG AAGCAAGTTG AAGATGGAGA AGTGTTTGAT 
TTCAGAGGGA TGAGATTAGA TTGGTTTAGG TTACAGGCGT ACACTAGCGT CTCCAAAGCT 
TCACTTAGCC TTGCAGATCA CAGAGAACTT GGAAAGATGA TGAACACAAT CATATTCCAT 
ACAAAAATGG TAGATTCCTT GGTTGAAATG TTGGTGGAAA CATCCGATCT CTCCATATTT 
TGTTTTTACA GCCGTGCTTT TGAGAAGATG TTTCAGCAGT GCTTGGAGCT GCCCTCTCAG 
TCAAGATACT CCATTGCCTT TCCTCTGCTC TGCACTCACT TCATGAGCTG CACACATGAA 
CTGTGTCCTG AAGAGCGCCA TCATATAGGA GATCGTAGCC TTTCCCTGTG CAATATGTTC 
CTGGATGAGA TGGCCAAACA AGCTCGAAAT CTCATCACCG ATATTTGCAC AGAACAGTGT 
ACTCTTAGTG ACCAGTTATT GCCGAAGCAT TGTGCCAAAA CCATCAGTCA AGCAGTGAAT 
AAGAAGTCAA AAAAACAGAC GGGCAAGAAA GGGGAACCTG AAAGGGAAAA ACCGGGTGTG 
GAGAGCATGA GGAAAAACAG GCTGGTAGTG ACCAACCTTG ATAAGTTGCA CACTGCACTT 
TCGGAGTTAT GCTTCTCCAT AAATTATGTT CCAAACATGG CGGTGTGGGA GCACACCTTC 
ACGCCGAGGG AGTATTTGAC TTCTCATCTG GAAATCCGGT TCACTAAATC AATTGTTGGA 
ATGACTATGT ATAATCAAGC TACACAGGAA ATTGCAAAGC CATCAGAGCT TCTAACAAGC 
GTAAGAGCAT ATATGACTGT ACTCCAGTCC ATAGAGAACT ATGTGCAGAT TGATATCACC 
AGAGTATTTA ATAATGTCCT TCTTCAGCAA ACACAACACT TAGACAGCCA TGGAGAACCA 
ACCATCACGA GTCTGTATAC AAACTGGTAC TTGGAAACTT TATTAAGACA AGTCAGCAAT 
GGCCATATAG CTTATTTTCC TGCAATGAAA GCATTTGTGA ATTTACCCAC AGAAAATGAA 
TTAACATTCA ATGCAGAGGA ATATTCTGAT ATATCAGAAA TGAGATCATT ATCAGAGCTC 
CTAGGCCCAT ATGGGATGAA GTTCCTGAGT GAGAGCCTTA TGTGGCACAT TTCATCACAA 
GTAGCTGAAC TTAAGAAACT TGTGGTGGAG AATGTTGATG TTCTAACACA AATGAGGACC 
AGCTTTGACA AACCAGACCA GATGGCTGCA CTCTTTAAAA GATTATCATC TGTTGACAGT 
GTCTTGAAAA GGATGACGAT AATTGGTGTT ATTTTATCCT TCCGGTCATT GGCACAAGAA 
GCATTGAGAG ATGTCCTGTC CTACCACATT CCTTTCCTCG TGAGCTCCAT TGAAGATTTC 
AAGGATCACA TTCCTCGGGA AACTGATATG AAGGTTGCAA TGAATGTGTA TGAGCTGTCC 
TCGGCTGCTG GGTTACCATG TGAGATTGAT CCTGCCTTAG TCGTAGCTCT TTCCTCACAA 
AAATCAGAGA ACATTAGTCC AGAGGAAGAA TACAAGATCG CTTGTCTCCT GATGGTCTTT 
GTAGCAGTTT CCTTGCCTAC ACTGGCCAGC AATGTAATGT CACAGTACAG CCCTGCAATA 
GAAGGACACT GCAACAATAT ACATTGTTTG GCCAAAGCCA TCAACCAGAT TGCTGCAGCT 
TTGTTTACAA TTCACAAAGG AAGTATTGAA GACCGACTTA AGGAATTCCT GGCGCTTGCA 
TCATCCAGTC TACTGAAAAT TGGCCAAGAG ACAGATAAAA CTACAACAAG AAATAGAGAA 
TCTGTTTATT TGCTACTGGA TATGATTGTA CAAGAATCAC CATTCCTGAC AATGGATCTC 
TTGGAATCTT GTTTTCCTTA TGTGTTGCTG AGAAATGCAT ACCATGCTGT CTACAAACAA 
AGTGTTACAT CTTCTGCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NCK Associated Protein 1 Modulated by miRNA-214 Determines Vascular Smooth Muscle Cell Migration, Proliferation, and Neointima Hyperplasia.
Journal of the American Heart Association5(12)(2016 Dec)
Afzal TA,Luong LA,Chen D,Zhang C,Yang F,Chen Q,An W,Wilkes E,Yashiro K,Cutillas PR,Zhang L,Xiao Q

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Loss of Sip1 leads to migration defects and retention of ectodermal markers during lens development.
Mechanisms of development13186-110(2014 Feb)
Manthey AL,Lachke SA,FitzGerald PG,Mason RW,Scheiblin DA,McDonald JH,Duncan MK

CYFIP1 coordinates mRNA translation and cytoskeleton remodeling to ensure proper dendritic spine formation.
Neuron79(6)1169-82(2013 Sep)
De Rubeis S,Pasciuto E,Li KW,Fern?ndez E,Di Marino D,Buzzi A,Ostroff LE,Klann E,Zwartkruis FJ,Komiyama NH,Grant SG,Poujol C,Choquet D,Achsel T,Posthuma D,Smit AB,Bagni C

NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons.
The EMBO journal30(23)4739-54(2011 Sep)
Yokoyama K,Tezuka T,Kotani M,Nakazawa T,Hoshina N,Shimoda Y,Kakuta S,Sudo K,Watanabe K,Iwakura Y,Yamamoto T

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Nckap1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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