Pnpla6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pnpla6
Entrez Gene ID	50767
Full Name	patatin-like phospholipase domain containing 6
Synonyms	AI661849,MSws,Nte
General protein information	Preferred Names patatin-like phospholipase domain containing 6 Names neuropathy target esterase Swiss-cheese patatin-like phospholipase domain-containing protein 6
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 A1.1\|8 1.92 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006508823.3	XP_006508886.1	neuropathy target esterase isoform X2
XM_006508826.3	XP_006508889.1	neuropathy target esterase isoform X5
XM_017312890.1	XP_017168379.1	neuropathy target esterase isoform X6
XM_006508825.3	XP_006508888.2	neuropathy target esterase isoform X4
XM_006508829.1	XP_006508892.1	neuropathy target esterase isoform X5
XM_011242066.2	XP_011240368.1	neuropathy target esterase isoform X8
NM_001359123.1	NP_001346052.1	neuropathy target esterase isoform 5
NM_001359124.1	NP_001346053.1	neuropathy target esterase isoform 6
NM_001122818.2	NP_001116290.2	neuropathy target esterase isoform 1
XM_006508824.4	XP_006508887.1	neuropathy target esterase isoform X1
XM_006508827.4	XP_006508890.1	neuropathy target esterase isoform X3
XM_006508831.3	XP_006508894.1	neuropathy target esterase isoform X7
XM_006508832.3	XP_006508895.1	neuropathy target esterase isoform X10
XM_006508829.2	XP_006508892.1	neuropathy target esterase isoform X3
XM_006508830.4	XP_006508893.3	neuropathy target esterase isoform X4
XM_006508827.3	XP_006508890.1	neuropathy target esterase isoform X5
XM_011242066.3	XP_011240368.1	neuropathy target esterase isoform X6
XM_006508832.4	XP_006508895.1	neuropathy target esterase isoform X7
NM_001359122.1	NP_001346051.1	neuropathy target esterase isoform 4
NM_001359121.1	NP_001346050.1	neuropathy target esterase isoform 3
XM_006508822.3	XP_006508885.1	neuropathy target esterase isoform X1
XM_017312891.1	XP_017168380.1	neuropathy target esterase isoform X9
XM_006508824.3	XP_006508887.1	neuropathy target esterase isoform X3
XM_006508830.3	XP_006508893.2	neuropathy target esterase isoform X6
XM_006508828.1	XP_006508891.1	neuropathy target esterase isoform X3
NM_015801.2	NP_056616.2	neuropathy target esterase isoform 2
XM_006508831.2	XP_006508894.1	neuropathy target esterase isoform X1
XM_006508825.4	XP_006508888.3	neuropathy target esterase isoform X2
XM_006508831.4	XP_006508894.1	neuropathy target esterase isoform X5

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PNPLA6	NP_001070015.2
Homo sapiens (human)	PNPLA6	NP_001159583.1
Danio rerio (zebrafish)	LOC560986	XP_005160109.1
Canis lupus familiaris (dog)	PNPLA6	XP_005632988.1
Mus musculus (house mouse)	Pnpla6	NP_001116290.2
Rattus norvegicus (Norway rat)	Pnpla6	XP_341026.6
Gallus gallus (chicken)	PNPLA6	NP_001106924.1
Pan troglodytes (chimpanzee)	PNPLA6	XP_512331.3

Gene Ontology
Bibliography

Related articles in PubMed

NTE/PNPLA6 is expressed in mature Schwann cells and is required for glial ensheathment of Remak fibers.
McFerrin J, Patton BL, Sunderhaus ER, Kretzschmar D
Glia65(5)804-816(2017 May)

Expression of Neuropathy Target Esterase in mouse embryonic stem cells during differentiation.
Pamies D, Reig JA, Vilanova E, Sogorb MA
Archives of toxicology84(6)481-91(2010 Jun)

Spongiform pathology in mouse CNS lacking 'neuropathy target esterase' and cellular prion protein.
Rosenbluth J, Schiff R, Lam P, Nuriel T, Chao MV
Neurobiology of disease35(3)433-7(2009 Sep)

Neuropathy target esterase is required for adult vertebrate axon maintenance.
Read DJ, Li Y, Chao MV, Cavanagh JB, Glynn P
The Journal of neuroscience : the official journal of the Society for Neuroscience29(37)11594-600(2009 Sep)

Neuropathy target esterase catalyzes osmoprotective renal synthesis of glycerophosphocholine in response to high NaCl.
Gallazzini M, Ferraris JD, Kunin M, Morris RG, Burg MB
Proceedings of the National Academy of Sciences of the United States of America103(41)15260-5(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the expression of CaMKIIalpha and pCREB exhibits a significant increase in cortex and hippocampus after treatment with PFOS, compared with the control.
Title: Expression of Neuropathy Target Esterase in mouse embryonic stem cells during differentiation.

We conclude that spongiform pathology in conditional NTE knockout mice is not mediated by PrPc, and that tubulovesicular inclusions can be seen in spongiform encephalopathy of other etiologies and are not pathognomonic of prion disease
Title: Spongiform pathology in mouse CNS lacking 'neuropathy target esterase' and cellular prion protein.

Increase of neuropathy target esterase (NTE) expression contributes to increased production and accumulation of glycerophosphocholine in mammalian renal cells in tissue culture and in vivo.
Title: Neuropathy target esterase catalyzes osmoprotective renal synthesis of glycerophosphocholine in response to high NaCl.

role in the nervous system and indicate that a loss-of-function mechanism may contribute to neurodegenerative diseases characterized by vacuolation and neuronal loss
Title: Brain-specific deletion of neuropathy target esterase/swisscheese results in neurodegeneration.

impairment of vasculogenesis in the yolk sacs and embryos of null mutant conceptuses suggested that NTE is required for normal blood vessel development
Title: Placental failure and impaired vasculogenesis result in embryonic lethality for neuropathy target esterase-deficient mice.

The following Pnpla6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pnpla6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu82535	NM_001359121.1 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OMu82535	XM_017312890.1 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OMu82535	XM_006508830.3 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OMu05750	NM_001122818.2 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OMu05750	XM_006508831.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu40424	XM_006508824.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu40425	XM_006508825.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu40426	XM_006508827.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu40426	XM_006508829.2 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu40427	XM_006508830.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu40428	XM_006508832.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu69375	XM_011242066.3 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu82536	XM_017312891.1 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu10290	NM_015801.2 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$776.30 $1109.00
OMu10290	XM_006508825.3 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$776.30 $1109.00
OMu40422	NM_001359123.1 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu40423	NM_001359124.1 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu40426	NM_001359122.1 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu40422	XM_006508822.3 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu40423	XM_006508823.3 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu40426	XM_006508826.3 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu40426	XM_006508828.1 Latest version!	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu40426	XM_006508829.1	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu40426	XM_006508827.3	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu69375	XM_011242066.2	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40428	XM_006508832.3	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40424	XM_006508824.3	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu05750	XM_006508831.2	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OMu05750	XM_006508831.3	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pnpla6 ( NM_001359121.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346050.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu82535
Clone ID Related Accession (Same CDS sequence)	XM_017312890.1 , NM_001359121.1 , XM_006508830.3
Accession Version	NM_001359121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC170806.2. On Dec 21, 2017 this sequence version replaced XM_017312890.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.60375.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCCC CACTGCAAAC CGGAATGGTG CTGGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGCTGGTCA CCGCCGTGCT CATCCTCTTG GTGGTGCGAA GGCTGCGGGT GCAGAAAACT 
CCAGCCCCGG AGGGTCCCCG GTATCGGTTC CGGAAGCGGG ACAAAGTGCT TTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAGTCC ACTTCTTCCC TGGTGGACAC ATCAGTCTCC 
ACCACTTCCC GGCCCCGCAT GAAGAAGAAA CTGAAGATGC TTAACATTGC CAAGAAGATC 
TTACGTATCC AAAAGGAGAC TCCGACCCTA CAACGGAAGG AGCCCCCACC TTCAGTGCTG 
GAGGCTGACC TCACAGAGGG AGACCTGGCC AATTCCCACC TACCCTCTGA GGTACTCTAC 
ATGCTTAAGA ATGTTCGGGT GCTAGGTCAC TTTGAGAAGC CGCTCTTCCT GGAGCTCTGC 
CGGCACATGG TCTTCCAGCG TCTCGGGCAG GGGGACTATG TTTTTCGGCC CGGTCAACCA 
GATGCCAGCA TCTATGTGGT TCAAGATGGG CTGCTGGAGC TCTGCCTACC AGGGCCTGAT 
GGGAAGGAGT GTGTGGTGAA GGAGGTGGTT CCTGGAGACA GCGTCAACAG CCTTCTGAGC 
ATCCTTGATG TCATCACGGG TCACCAGCAT CCCCAGCGGA CTGTGTCAGC CAGGGCTGCA 
CGGGACTCCA CAGTGCTGAG ACTCCCAGTA GAAGCCTTCT CTGCTGTCTT CACCAAGTAC 
CCTGAGAGTT TGGTGCGAGT GGTACAGATC ATCATGGTAA GGCTGCAGAG AGTCACCTTC 
CTGGCGCTTC ACAATTACCT GGGTCTGACC AATGAACTCT TCAGTCATGA GATCCAGCCC 
CTACGCCTTT TCCCCAGCCC CGGCCTCCCG ACCCGAACCA GCCCTGTCCG TGGCTCTAAA 
CGGGTAGTCA GCACCTCAGG TACTGAGGAC ACCTCAAAGG AGACCTCTGG CCGGCCCCTG 
GACTCCATTG GAGCTCCTCT GCCTGGACCT GCAGGGGATC CCGTGAAGCC CACATCCTTA 
GAAGCACCCC CAGCCCCGTT ACTGAGCCGC TGCATCTCCA TGCCAGTAGA CATCTCAGGC 
TTGCAAGGTG GCCCTCGTTC TGATTTTGAC ATGGCCTATG AACGTGGACG GATATCTGTT 
TCTCTTCAAG AAGAGGCATC TGGGGGACCT CAGACAGCAT CCCCTAGGGA GCTCCGGGAG 
CAGCCAGCGG GTGCCTGTGA ATATAGCTAC TGTGAGGATG AGTCAGCCAC AGGCGGATGT 
CCCTTTGGGC CCTACCAGGG CCGCCAGACA AGCAGCATCT TTGAGGCTGC AAAGAGAGAG 
CTAGCCAAGC TGATGCGGAT TGAGGACCCC TCTCTACTGA ACAGCAGAGT CTTGCTACAT 
CATGCCAAAG CCGGCACCAT CATAGCCCGC CAAGGGGACC AGGATGTGAG CCTGCACTTT 
GTGCTCTGGG GCTGCCTGCA CGTGTACCAG CGCATGATTG ACAAGGCTGA GGAAGTGTGC 
CTATTTGTGG CACAGCCAGG GGAGCTGGTG GGGCAACTGG CGGTGCTCAC TGGGGAACCT 
CTCATCTTCA CACTACGTGC CCAGAGAGAC TGCACCTTCC TGCGGATCTC CAAATCCCAC 
TTCTATGAGA TCATGCGTGC ACAACCCAGT GTGGTACTGA GTGCAGCTCA CACGGTGGCT 
GCTAGAATGT CCCCCTTCGT GCGCCAGATG GACTTTGCCA TTGACTGGAC AGCTGTGGAG 
GCTGGTCGTG CGCTCTACAG GCAGGGAGAC CGCTCCGACT GCACCTACAT TGTACTCAAT 
GGACGTCTCC GTAGTGTCAT CCAACGAGGC AGTGGCAAGA AGGAGCTAGT TGGGGAGTAT 
GGTCGAGGGG ATCTCATTGG CGTGGTGGAG GCACTGACCC GGCAGCCACG AGCCACCACT 
GTACACGCAG TGCGAGACAC TGAGCTGGCC AAGCTTCCCG AGGGCACCTT AGGCCACATC 
AAACGTCGGT ACCCACAGGT TGTGACCCGC CTTATTCATC TGCTAAGCCA GAAAATTCTA 
GGCAATCTGC AGCAGTTGCA AGGACCCTTT CCAGGCTCCG GGCTCAGTGT TCCGCAGCAC 
TCGGAACTGA CCAACCCAGC CAGCAATCTG TCTACTGTAG CTATCCTCCC TGTGTGTGCT 
GAGGTGCCCA TGATGGCCTT TACTCTGGAA CTGCAGCATG CTCTGCAAGC TATTGGTCCC 
ACGCTCCTCC TTAACAGTGA CGTCATCCGG GCACTCCTGG GGGCTTCAGC ACTGGATAGC 
ATTCAAGAAT TCCGGCTGTC AGGGTGGTTG GCCCAGCAGG AAGATGCCCA TCGCATTGTG 
CTCTACCAAA CTGACACATC CCTGACGCCT TGGACCGTCC GTTGTCTGCG CCAGGCCGAC 
TGCATCCTCA TTGTGGGCCT GGGGGACCAG GAACCCACTG TTGGCCAGCT GGAGCAAATG 
TTAGAGAACA CTGCTGTCCG TGCCTTGAAG CAACTGGTTT TGCTGCACCG GGAGGAAGGC 
CCTGGTCCCA CGCGCACTGT AGAGTGGCTC AACATGCGCA GCTGGTGCTC AGGGCACCTG 
CACCTGCGCT GTCCTCGCCG CCTCTTCTCA CGGCGCAGCC CAGCGAAACT TCATGAGCTG 
TATGAGAAGG TTTTCTCCAG GCGTGCAGAC CGTCACAGCG ACTTCTCCCG CTTGGCACGA 
GTACTCACAG GAAACACTAT TGCTCTGGTG CTGGGTGGGG GCGGAGCCAG AGGCTGCTCG 
CATATTGGGG TGCTGAAGGC ATTAGAGGAG GCAGGAGTCC CAGTCGACCT TGTGGGAGGC 
ACATCCATAG GTTCCTTCAT TGGGGCCTTG TATGCCGAGG AACGCAGCGC CAGCCGAACT 
AAACAACGAG CCCGGGAGTG GGCCAAGAGC ATGACTTCTG TACTGGAGCC TGTATTGGAC 
CTCACATATC CTGTCACCTC CATGTTTACT GGCTCAGCCT TTAACAGAAG TATCCACCGT 
GTCTTCCAGG ATAAGCAGAT TGAGGACCTG TGGCTGCCTT ACTTCAATGT GACCACAGAC 
ATCACTGCCT CTGCCATGCG CGTCCACAAA GATGGCTCCC TGTGGCGGTA TGTACGTGCC 
AGCATGACGC TCTCGGGCTA CCTACCCCCG CTGTGCGACC CAAAGGATGG GCACCTGCTC 
ATGGATGGTG GCTACATCAA CAACCTGCCA GCGGATATTG CCCGAAGCAT GGGAGCCAAA 
ACGGTCATTG CCATCGACGT TGGAAGCCAG GATGAGACAG ATCTCAGCAC CTATGGGGAC 
AGCCTGTCTG GCTGGTGGTT GCTGTGGAAA AGGCTAAACC CCTGGGCAGA CAAGGTAAAG 
GTTCCAGACA TGGCTGAGAT CCAGTCCCGC TTGGCGTACG TGTCCTGTGT GCGGCAGCTA 
GAAGTCGTCA AGTCCAGCTC CTATTGCGAG TACCTTCGTC CATCCATTGA CTGCTTCAAG 
ACCATGGACT TTGGAAAGTT TGACCAGATC TATGATGTGG GTTACCAGTA TGGGAAGGCT 
GTCTTTGGAG GCTGGACCCG TGGTGAAGTC ATTGAGAAAA TGCTCACAGA CCGGAGATCT 
ACAGACCTTA ATGAGAGTCG CCGTGCAGAT ATACTTGCCT TCCCAAGTTC TGGGTTCACT 
GACTTGGCTG AGATTGTGTC CCGGATCGAG CCACCAACAA GCTACGTCTC TGATGGCTGT 
GCTGATGGGG AGGAGTCGGA TTGCTTGACA GAGTATGAGG AAGATGCAGG ACCAGACTGC 
TCAAGAGATG AAGGGGGCTC CCCTGAGGGT GCCAGCCCTA GCACTGCCTC AGAGGTGGAA 
GAAGAGAAGT CCACACTCCG GCAACGACGC TTTCTGCCTC AGGAGACTCC CAGCTCAGTC 
GCAGATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346050.1
CDS	216..4187
Translation	MEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	NM_001359121.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359121.1

ORF Insert Sequence:

ATGGAGGCCC CACTGCAAAC CGGAATGGTG CTGGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGCTGGTCA CCGCCGTGCT CATCCTCTTG GTGGTGCGAA GGCTGCGGGT GCAGAAAACT 
CCAGCCCCGG AGGGTCCCCG GTATCGGTTC CGGAAGCGGG ACAAAGTGCT TTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAGTCC ACTTCTTCCC TGGTGGACAC ATCAGTCTCC 
ACCACTTCCC GGCCCCGCAT GAAGAAGAAA CTGAAGATGC TTAACATTGC CAAGAAGATC 
TTACGTATCC AAAAGGAGAC TCCGACCCTA CAACGGAAGG AGCCCCCACC TTCAGTGCTG 
GAGGCTGACC TCACAGAGGG AGACCTGGCC AATTCCCACC TACCCTCTGA GGTACTCTAC 
ATGCTTAAGA ATGTTCGGGT GCTAGGTCAC TTTGAGAAGC CGCTCTTCCT GGAGCTCTGC 
CGGCACATGG TCTTCCAGCG TCTCGGGCAG GGGGACTATG TTTTTCGGCC CGGTCAACCA 
GATGCCAGCA TCTATGTGGT TCAAGATGGG CTGCTGGAGC TCTGCCTACC AGGGCCTGAT 
GGGAAGGAGT GTGTGGTGAA GGAGGTGGTT CCTGGAGACA GCGTCAACAG CCTTCTGAGC 
ATCCTTGATG TCATCACGGG TCACCAGCAT CCCCAGCGGA CTGTGTCAGC CAGGGCTGCA 
CGGGACTCCA CAGTGCTGAG ACTCCCAGTA GAAGCCTTCT CTGCTGTCTT CACCAAGTAC 
CCTGAGAGTT TGGTGCGAGT GGTACAGATC ATCATGGTAA GGCTGCAGAG AGTCACCTTC 
CTGGCGCTTC ACAATTACCT GGGTCTGACC AATGAACTCT TCAGTCATGA GATCCAGCCC 
CTACGCCTTT TCCCCAGCCC CGGCCTCCCG ACCCGAACCA GCCCTGTCCG TGGCTCTAAA 
CGGGTAGTCA GCACCTCAGG TACTGAGGAC ACCTCAAAGG AGACCTCTGG CCGGCCCCTG 
GACTCCATTG GAGCTCCTCT GCCTGGACCT GCAGGGGATC CCGTGAAGCC CACATCCTTA 
GAAGCACCCC CAGCCCCGTT ACTGAGCCGC TGCATCTCCA TGCCAGTAGA CATCTCAGGC 
TTGCAAGGTG GCCCTCGTTC TGATTTTGAC ATGGCCTATG AACGTGGACG GATATCTGTT 
TCTCTTCAAG AAGAGGCATC TGGGGGACCT CAGACAGCAT CCCCTAGGGA GCTCCGGGAG 
CAGCCAGCGG GTGCCTGTGA ATATAGCTAC TGTGAGGATG AGTCAGCCAC AGGCGGATGT 
CCCTTTGGGC CCTACCAGGG CCGCCAGACA AGCAGCATCT TTGAGGCTGC AAAGAGAGAG 
CTAGCCAAGC TGATGCGGAT TGAGGACCCC TCTCTACTGA ACAGCAGAGT CTTGCTACAT 
CATGCCAAAG CCGGCACCAT CATAGCCCGC CAAGGGGACC AGGATGTGAG CCTGCACTTT 
GTGCTCTGGG GCTGCCTGCA CGTGTACCAG CGCATGATTG ACAAGGCTGA GGAAGTGTGC 
CTATTTGTGG CACAGCCAGG GGAGCTGGTG GGGCAACTGG CGGTGCTCAC TGGGGAACCT 
CTCATCTTCA CACTACGTGC CCAGAGAGAC TGCACCTTCC TGCGGATCTC CAAATCCCAC 
TTCTATGAGA TCATGCGTGC ACAACCCAGT GTGGTACTGA GTGCAGCTCA CACGGTGGCT 
GCTAGAATGT CCCCCTTCGT GCGCCAGATG GACTTTGCCA TTGACTGGAC AGCTGTGGAG 
GCTGGTCGTG CGCTCTACAG GCAGGGAGAC CGCTCCGACT GCACCTACAT TGTACTCAAT 
GGACGTCTCC GTAGTGTCAT CCAACGAGGC AGTGGCAAGA AGGAGCTAGT TGGGGAGTAT 
GGTCGAGGGG ATCTCATTGG CGTGGTGGAG GCACTGACCC GGCAGCCACG AGCCACCACT 
GTACACGCAG TGCGAGACAC TGAGCTGGCC AAGCTTCCCG AGGGCACCTT AGGCCACATC 
AAACGTCGGT ACCCACAGGT TGTGACCCGC CTTATTCATC TGCTAAGCCA GAAAATTCTA 
GGCAATCTGC AGCAGTTGCA AGGACCCTTT CCAGGCTCCG GGCTCAGTGT TCCGCAGCAC 
TCGGAACTGA CCAACCCAGC CAGCAATCTG TCTACTGTAG CTATCCTCCC TGTGTGTGCT 
GAGGTGCCCA TGATGGCCTT TACTCTGGAA CTGCAGCATG CTCTGCAAGC TATTGGTCCC 
ACGCTCCTCC TTAACAGTGA CGTCATCCGG GCACTCCTGG GGGCTTCAGC ACTGGATAGC 
ATTCAAGAAT TCCGGCTGTC AGGGTGGTTG GCCCAGCAGG AAGATGCCCA TCGCATTGTG 
CTCTACCAAA CTGACACATC CCTGACGCCT TGGACCGTCC GTTGTCTGCG CCAGGCCGAC 
TGCATCCTCA TTGTGGGCCT GGGGGACCAG GAACCCACTG TTGGCCAGCT GGAGCAAATG 
TTAGAGAACA CTGCTGTCCG TGCCTTGAAG CAACTGGTTT TGCTGCACCG GGAGGAAGGC 
CCTGGTCCCA CGCGCACTGT AGAGTGGCTC AACATGCGCA GCTGGTGCTC AGGGCACCTG 
CACCTGCGCT GTCCTCGCCG CCTCTTCTCA CGGCGCAGCC CAGCGAAACT TCATGAGCTG 
TATGAGAAGG TTTTCTCCAG GCGTGCAGAC CGTCACAGCG ACTTCTCCCG CTTGGCACGA 
GTACTCACAG GAAACACTAT TGCTCTGGTG CTGGGTGGGG GCGGAGCCAG AGGCTGCTCG 
CATATTGGGG TGCTGAAGGC ATTAGAGGAG GCAGGAGTCC CAGTCGACCT TGTGGGAGGC 
ACATCCATAG GTTCCTTCAT TGGGGCCTTG TATGCCGAGG AACGCAGCGC CAGCCGAACT 
AAACAACGAG CCCGGGAGTG GGCCAAGAGC ATGACTTCTG TACTGGAGCC TGTATTGGAC 
CTCACATATC CTGTCACCTC CATGTTTACT GGCTCAGCCT TTAACAGAAG TATCCACCGT 
GTCTTCCAGG ATAAGCAGAT TGAGGACCTG TGGCTGCCTT ACTTCAATGT GACCACAGAC 
ATCACTGCCT CTGCCATGCG CGTCCACAAA GATGGCTCCC TGTGGCGGTA TGTACGTGCC 
AGCATGACGC TCTCGGGCTA CCTACCCCCG CTGTGCGACC CAAAGGATGG GCACCTGCTC 
ATGGATGGTG GCTACATCAA CAACCTGCCA GCGGATATTG CCCGAAGCAT GGGAGCCAAA 
ACGGTCATTG CCATCGACGT TGGAAGCCAG GATGAGACAG ATCTCAGCAC CTATGGGGAC 
AGCCTGTCTG GCTGGTGGTT GCTGTGGAAA AGGCTAAACC CCTGGGCAGA CAAGGTAAAG 
GTTCCAGACA TGGCTGAGAT CCAGTCCCGC TTGGCGTACG TGTCCTGTGT GCGGCAGCTA 
GAAGTCGTCA AGTCCAGCTC CTATTGCGAG TACCTTCGTC CATCCATTGA CTGCTTCAAG 
ACCATGGACT TTGGAAAGTT TGACCAGATC TATGATGTGG GTTACCAGTA TGGGAAGGCT 
GTCTTTGGAG GCTGGACCCG TGGTGAAGTC ATTGAGAAAA TGCTCACAGA CCGGAGATCT 
ACAGACCTTA ATGAGAGTCG CCGTGCAGAT ATACTTGCCT TCCCAAGTTC TGGGTTCACT 
GACTTGGCTG AGATTGTGTC CCGGATCGAG CCACCAACAA GCTACGTCTC TGATGGCTGT 
GCTGATGGGG AGGAGTCGGA TTGCTTGACA GAGTATGAGG AAGATGCAGG ACCAGACTGC 
TCAAGAGATG AAGGGGGCTC CCCTGAGGGT GCCAGCCCTA GCACTGCCTC AGAGGTGGAA 
GAAGAGAAGT CCACACTCCG GCAACGACGC TTTCTGCCTC AGGAGACTCC CAGCTCAGTC 
GCAGATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_017312890.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168379.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X11, mRNA.

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CloneID	OMu82535
Clone ID Related Accession (Same CDS sequence)	XM_017312890.1 , NM_001359121.1 , XM_006508830.3
Accession Version	XM_017312890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	neuropathy target esterase isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC CACTGCAAAC CGGAATGGTG CTGGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGCTGGTCA CCGCCGTGCT CATCCTCTTG GTGGTGCGAA GGCTGCGGGT GCAGAAAACT 
CCAGCCCCGG AGGGTCCCCG GTATCGGTTC CGGAAGCGGG ACAAAGTGCT TTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAGTCC ACTTCTTCCC TGGTGGACAC ATCAGTCTCC 
ACCACTTCCC GGCCCCGCAT GAAGAAGAAA CTGAAGATGC TTAACATTGC CAAGAAGATC 
TTACGTATCC AAAAGGAGAC TCCGACCCTA CAACGGAAGG AGCCCCCACC TTCAGTGCTG 
GAGGCTGACC TCACAGAGGG AGACCTGGCC AATTCCCACC TACCCTCTGA GGTACTCTAC 
ATGCTTAAGA ATGTTCGGGT GCTAGGTCAC TTTGAGAAGC CGCTCTTCCT GGAGCTCTGC 
CGGCACATGG TCTTCCAGCG TCTCGGGCAG GGGGACTATG TTTTTCGGCC CGGTCAACCA 
GATGCCAGCA TCTATGTGGT TCAAGATGGG CTGCTGGAGC TCTGCCTACC AGGGCCTGAT 
GGGAAGGAGT GTGTGGTGAA GGAGGTGGTT CCTGGAGACA GCGTCAACAG CCTTCTGAGC 
ATCCTTGATG TCATCACGGG TCACCAGCAT CCCCAGCGGA CTGTGTCAGC CAGGGCTGCA 
CGGGACTCCA CAGTGCTGAG ACTCCCAGTA GAAGCCTTCT CTGCTGTCTT CACCAAGTAC 
CCTGAGAGTT TGGTGCGAGT GGTACAGATC ATCATGGTAA GGCTGCAGAG AGTCACCTTC 
CTGGCGCTTC ACAATTACCT GGGTCTGACC AATGAACTCT TCAGTCATGA GATCCAGCCC 
CTACGCCTTT TCCCCAGCCC CGGCCTCCCG ACCCGAACCA GCCCTGTCCG TGGCTCTAAA 
CGGGTAGTCA GCACCTCAGG TACTGAGGAC ACCTCAAAGG AGACCTCTGG CCGGCCCCTG 
GACTCCATTG GAGCTCCTCT GCCTGGACCT GCAGGGGATC CCGTGAAGCC CACATCCTTA 
GAAGCACCCC CAGCCCCGTT ACTGAGCCGC TGCATCTCCA TGCCAGTAGA CATCTCAGGC 
TTGCAAGGTG GCCCTCGTTC TGATTTTGAC ATGGCCTATG AACGTGGACG GATATCTGTT 
TCTCTTCAAG AAGAGGCATC TGGGGGACCT CAGACAGCAT CCCCTAGGGA GCTCCGGGAG 
CAGCCAGCGG GTGCCTGTGA ATATAGCTAC TGTGAGGATG AGTCAGCCAC AGGCGGATGT 
CCCTTTGGGC CCTACCAGGG CCGCCAGACA AGCAGCATCT TTGAGGCTGC AAAGAGAGAG 
CTAGCCAAGC TGATGCGGAT TGAGGACCCC TCTCTACTGA ACAGCAGAGT CTTGCTACAT 
CATGCCAAAG CCGGCACCAT CATAGCCCGC CAAGGGGACC AGGATGTGAG CCTGCACTTT 
GTGCTCTGGG GCTGCCTGCA CGTGTACCAG CGCATGATTG ACAAGGCTGA GGAAGTGTGC 
CTATTTGTGG CACAGCCAGG GGAGCTGGTG GGGCAACTGG CGGTGCTCAC TGGGGAACCT 
CTCATCTTCA CACTACGTGC CCAGAGAGAC TGCACCTTCC TGCGGATCTC CAAATCCCAC 
TTCTATGAGA TCATGCGTGC ACAACCCAGT GTGGTACTGA GTGCAGCTCA CACGGTGGCT 
GCTAGAATGT CCCCCTTCGT GCGCCAGATG GACTTTGCCA TTGACTGGAC AGCTGTGGAG 
GCTGGTCGTG CGCTCTACAG GCAGGGAGAC CGCTCCGACT GCACCTACAT TGTACTCAAT 
GGACGTCTCC GTAGTGTCAT CCAACGAGGC AGTGGCAAGA AGGAGCTAGT TGGGGAGTAT 
GGTCGAGGGG ATCTCATTGG CGTGGTGGAG GCACTGACCC GGCAGCCACG AGCCACCACT 
GTACACGCAG TGCGAGACAC TGAGCTGGCC AAGCTTCCCG AGGGCACCTT AGGCCACATC 
AAACGTCGGT ACCCACAGGT TGTGACCCGC CTTATTCATC TGCTAAGCCA GAAAATTCTA 
GGCAATCTGC AGCAGTTGCA AGGACCCTTT CCAGGCTCCG GGCTCAGTGT TCCGCAGCAC 
TCGGAACTGA CCAACCCAGC CAGCAATCTG TCTACTGTAG CTATCCTCCC TGTGTGTGCT 
GAGGTGCCCA TGATGGCCTT TACTCTGGAA CTGCAGCATG CTCTGCAAGC TATTGGTCCC 
ACGCTCCTCC TTAACAGTGA CGTCATCCGG GCACTCCTGG GGGCTTCAGC ACTGGATAGC 
ATTCAAGAAT TCCGGCTGTC AGGGTGGTTG GCCCAGCAGG AAGATGCCCA TCGCATTGTG 
CTCTACCAAA CTGACACATC CCTGACGCCT TGGACCGTCC GTTGTCTGCG CCAGGCCGAC 
TGCATCCTCA TTGTGGGCCT GGGGGACCAG GAACCCACTG TTGGCCAGCT GGAGCAAATG 
TTAGAGAACA CTGCTGTCCG TGCCTTGAAG CAACTGGTTT TGCTGCACCG GGAGGAAGGC 
CCTGGTCCCA CGCGCACTGT AGAGTGGCTC AACATGCGCA GCTGGTGCTC AGGGCACCTG 
CACCTGCGCT GTCCTCGCCG CCTCTTCTCA CGGCGCAGCC CAGCGAAACT TCATGAGCTG 
TATGAGAAGG TTTTCTCCAG GCGTGCAGAC CGTCACAGCG ACTTCTCCCG CTTGGCACGA 
GTACTCACAG GAAACACTAT TGCTCTGGTG CTGGGTGGGG GCGGAGCCAG AGGCTGCTCG 
CATATTGGGG TGCTGAAGGC ATTAGAGGAG GCAGGAGTCC CAGTCGACCT TGTGGGAGGC 
ACATCCATAG GTTCCTTCAT TGGGGCCTTG TATGCCGAGG AACGCAGCGC CAGCCGAACT 
AAACAACGAG CCCGGGAGTG GGCCAAGAGC ATGACTTCTG TACTGGAGCC TGTATTGGAC 
CTCACATATC CTGTCACCTC CATGTTTACT GGCTCAGCCT TTAACAGAAG TATCCACCGT 
GTCTTCCAGG ATAAGCAGAT TGAGGACCTG TGGCTGCCTT ACTTCAATGT GACCACAGAC 
ATCACTGCCT CTGCCATGCG CGTCCACAAA GATGGCTCCC TGTGGCGGTA TGTACGTGCC 
AGCATGACGC TCTCGGGCTA CCTACCCCCG CTGTGCGACC CAAAGGATGG GCACCTGCTC 
ATGGATGGTG GCTACATCAA CAACCTGCCA GCGGATATTG CCCGAAGCAT GGGAGCCAAA 
ACGGTCATTG CCATCGACGT TGGAAGCCAG GATGAGACAG ATCTCAGCAC CTATGGGGAC 
AGCCTGTCTG GCTGGTGGTT GCTGTGGAAA AGGCTAAACC CCTGGGCAGA CAAGGTAAAG 
GTTCCAGACA TGGCTGAGAT CCAGTCCCGC TTGGCGTACG TGTCCTGTGT GCGGCAGCTA 
GAAGTCGTCA AGTCCAGCTC CTATTGCGAG TACCTTCGTC CATCCATTGA CTGCTTCAAG 
ACCATGGACT TTGGAAAGTT TGACCAGATC TATGATGTGG GTTACCAGTA TGGGAAGGCT 
GTCTTTGGAG GCTGGACCCG TGGTGAAGTC ATTGAGAAAA TGCTCACAGA CCGGAGATCT 
ACAGACCTTA ATGAGAGTCG CCGTGCAGAT ATACTTGCCT TCCCAAGTTC TGGGTTCACT 
GACTTGGCTG AGATTGTGTC CCGGATCGAG CCACCAACAA GCTACGTCTC TGATGGCTGT 
GCTGATGGGG AGGAGTCGGA TTGCTTGACA GAGTATGAGG AAGATGCAGG ACCAGACTGC 
TCAAGAGATG AAGGGGGCTC CCCTGAGGGT GCCAGCCCTA GCACTGCCTC AGAGGTGGAA 
GAAGAGAAGT CCACACTCCG GCAACGACGC TTTCTGCCTC AGGAGACTCC CAGCTCAGTC 
GCAGATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168379.1
CDS	262..4233
Translation	MEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_017312890.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312890.1

ORF Insert Sequence:

ATGGAGGCCC CACTGCAAAC CGGAATGGTG CTGGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGCTGGTCA CCGCCGTGCT CATCCTCTTG GTGGTGCGAA GGCTGCGGGT GCAGAAAACT 
CCAGCCCCGG AGGGTCCCCG GTATCGGTTC CGGAAGCGGG ACAAAGTGCT TTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAGTCC ACTTCTTCCC TGGTGGACAC ATCAGTCTCC 
ACCACTTCCC GGCCCCGCAT GAAGAAGAAA CTGAAGATGC TTAACATTGC CAAGAAGATC 
TTACGTATCC AAAAGGAGAC TCCGACCCTA CAACGGAAGG AGCCCCCACC TTCAGTGCTG 
GAGGCTGACC TCACAGAGGG AGACCTGGCC AATTCCCACC TACCCTCTGA GGTACTCTAC 
ATGCTTAAGA ATGTTCGGGT GCTAGGTCAC TTTGAGAAGC CGCTCTTCCT GGAGCTCTGC 
CGGCACATGG TCTTCCAGCG TCTCGGGCAG GGGGACTATG TTTTTCGGCC CGGTCAACCA 
GATGCCAGCA TCTATGTGGT TCAAGATGGG CTGCTGGAGC TCTGCCTACC AGGGCCTGAT 
GGGAAGGAGT GTGTGGTGAA GGAGGTGGTT CCTGGAGACA GCGTCAACAG CCTTCTGAGC 
ATCCTTGATG TCATCACGGG TCACCAGCAT CCCCAGCGGA CTGTGTCAGC CAGGGCTGCA 
CGGGACTCCA CAGTGCTGAG ACTCCCAGTA GAAGCCTTCT CTGCTGTCTT CACCAAGTAC 
CCTGAGAGTT TGGTGCGAGT GGTACAGATC ATCATGGTAA GGCTGCAGAG AGTCACCTTC 
CTGGCGCTTC ACAATTACCT GGGTCTGACC AATGAACTCT TCAGTCATGA GATCCAGCCC 
CTACGCCTTT TCCCCAGCCC CGGCCTCCCG ACCCGAACCA GCCCTGTCCG TGGCTCTAAA 
CGGGTAGTCA GCACCTCAGG TACTGAGGAC ACCTCAAAGG AGACCTCTGG CCGGCCCCTG 
GACTCCATTG GAGCTCCTCT GCCTGGACCT GCAGGGGATC CCGTGAAGCC CACATCCTTA 
GAAGCACCCC CAGCCCCGTT ACTGAGCCGC TGCATCTCCA TGCCAGTAGA CATCTCAGGC 
TTGCAAGGTG GCCCTCGTTC TGATTTTGAC ATGGCCTATG AACGTGGACG GATATCTGTT 
TCTCTTCAAG AAGAGGCATC TGGGGGACCT CAGACAGCAT CCCCTAGGGA GCTCCGGGAG 
CAGCCAGCGG GTGCCTGTGA ATATAGCTAC TGTGAGGATG AGTCAGCCAC AGGCGGATGT 
CCCTTTGGGC CCTACCAGGG CCGCCAGACA AGCAGCATCT TTGAGGCTGC AAAGAGAGAG 
CTAGCCAAGC TGATGCGGAT TGAGGACCCC TCTCTACTGA ACAGCAGAGT CTTGCTACAT 
CATGCCAAAG CCGGCACCAT CATAGCCCGC CAAGGGGACC AGGATGTGAG CCTGCACTTT 
GTGCTCTGGG GCTGCCTGCA CGTGTACCAG CGCATGATTG ACAAGGCTGA GGAAGTGTGC 
CTATTTGTGG CACAGCCAGG GGAGCTGGTG GGGCAACTGG CGGTGCTCAC TGGGGAACCT 
CTCATCTTCA CACTACGTGC CCAGAGAGAC TGCACCTTCC TGCGGATCTC CAAATCCCAC 
TTCTATGAGA TCATGCGTGC ACAACCCAGT GTGGTACTGA GTGCAGCTCA CACGGTGGCT 
GCTAGAATGT CCCCCTTCGT GCGCCAGATG GACTTTGCCA TTGACTGGAC AGCTGTGGAG 
GCTGGTCGTG CGCTCTACAG GCAGGGAGAC CGCTCCGACT GCACCTACAT TGTACTCAAT 
GGACGTCTCC GTAGTGTCAT CCAACGAGGC AGTGGCAAGA AGGAGCTAGT TGGGGAGTAT 
GGTCGAGGGG ATCTCATTGG CGTGGTGGAG GCACTGACCC GGCAGCCACG AGCCACCACT 
GTACACGCAG TGCGAGACAC TGAGCTGGCC AAGCTTCCCG AGGGCACCTT AGGCCACATC 
AAACGTCGGT ACCCACAGGT TGTGACCCGC CTTATTCATC TGCTAAGCCA GAAAATTCTA 
GGCAATCTGC AGCAGTTGCA AGGACCCTTT CCAGGCTCCG GGCTCAGTGT TCCGCAGCAC 
TCGGAACTGA CCAACCCAGC CAGCAATCTG TCTACTGTAG CTATCCTCCC TGTGTGTGCT 
GAGGTGCCCA TGATGGCCTT TACTCTGGAA CTGCAGCATG CTCTGCAAGC TATTGGTCCC 
ACGCTCCTCC TTAACAGTGA CGTCATCCGG GCACTCCTGG GGGCTTCAGC ACTGGATAGC 
ATTCAAGAAT TCCGGCTGTC AGGGTGGTTG GCCCAGCAGG AAGATGCCCA TCGCATTGTG 
CTCTACCAAA CTGACACATC CCTGACGCCT TGGACCGTCC GTTGTCTGCG CCAGGCCGAC 
TGCATCCTCA TTGTGGGCCT GGGGGACCAG GAACCCACTG TTGGCCAGCT GGAGCAAATG 
TTAGAGAACA CTGCTGTCCG TGCCTTGAAG CAACTGGTTT TGCTGCACCG GGAGGAAGGC 
CCTGGTCCCA CGCGCACTGT AGAGTGGCTC AACATGCGCA GCTGGTGCTC AGGGCACCTG 
CACCTGCGCT GTCCTCGCCG CCTCTTCTCA CGGCGCAGCC CAGCGAAACT TCATGAGCTG 
TATGAGAAGG TTTTCTCCAG GCGTGCAGAC CGTCACAGCG ACTTCTCCCG CTTGGCACGA 
GTACTCACAG GAAACACTAT TGCTCTGGTG CTGGGTGGGG GCGGAGCCAG AGGCTGCTCG 
CATATTGGGG TGCTGAAGGC ATTAGAGGAG GCAGGAGTCC CAGTCGACCT TGTGGGAGGC 
ACATCCATAG GTTCCTTCAT TGGGGCCTTG TATGCCGAGG AACGCAGCGC CAGCCGAACT 
AAACAACGAG CCCGGGAGTG GGCCAAGAGC ATGACTTCTG TACTGGAGCC TGTATTGGAC 
CTCACATATC CTGTCACCTC CATGTTTACT GGCTCAGCCT TTAACAGAAG TATCCACCGT 
GTCTTCCAGG ATAAGCAGAT TGAGGACCTG TGGCTGCCTT ACTTCAATGT GACCACAGAC 
ATCACTGCCT CTGCCATGCG CGTCCACAAA GATGGCTCCC TGTGGCGGTA TGTACGTGCC 
AGCATGACGC TCTCGGGCTA CCTACCCCCG CTGTGCGACC CAAAGGATGG GCACCTGCTC 
ATGGATGGTG GCTACATCAA CAACCTGCCA GCGGATATTG CCCGAAGCAT GGGAGCCAAA 
ACGGTCATTG CCATCGACGT TGGAAGCCAG GATGAGACAG ATCTCAGCAC CTATGGGGAC 
AGCCTGTCTG GCTGGTGGTT GCTGTGGAAA AGGCTAAACC CCTGGGCAGA CAAGGTAAAG 
GTTCCAGACA TGGCTGAGAT CCAGTCCCGC TTGGCGTACG TGTCCTGTGT GCGGCAGCTA 
GAAGTCGTCA AGTCCAGCTC CTATTGCGAG TACCTTCGTC CATCCATTGA CTGCTTCAAG 
ACCATGGACT TTGGAAAGTT TGACCAGATC TATGATGTGG GTTACCAGTA TGGGAAGGCT 
GTCTTTGGAG GCTGGACCCG TGGTGAAGTC ATTGAGAAAA TGCTCACAGA CCGGAGATCT 
ACAGACCTTA ATGAGAGTCG CCGTGCAGAT ATACTTGCCT TCCCAAGTTC TGGGTTCACT 
GACTTGGCTG AGATTGTGTC CCGGATCGAG CCACCAACAA GCTACGTCTC TGATGGCTGT 
GCTGATGGGG AGGAGTCGGA TTGCTTGACA GAGTATGAGG AAGATGCAGG ACCAGACTGC 
TCAAGAGATG AAGGGGGCTC CCCTGAGGGT GCCAGCCCTA GCACTGCCTC AGAGGTGGAA 
GAAGAGAAGT CCACACTCCG GCAACGACGC TTTCTGCCTC AGGAGACTCC CAGCTCAGTC 
GCAGATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508830.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508893.2 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu82535
Clone ID Related Accession (Same CDS sequence)	XM_017312890.1 , NM_001359121.1 , XM_006508830.3
Accession Version	XM_006508830.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508830.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC CACTGCAAAC CGGAATGGTG CTGGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGCTGGTCA CCGCCGTGCT CATCCTCTTG GTGGTGCGAA GGCTGCGGGT GCAGAAAACT 
CCAGCCCCGG AGGGTCCCCG GTATCGGTTC CGGAAGCGGG ACAAAGTGCT TTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAGTCC ACTTCTTCCC TGGTGGACAC ATCAGTCTCC 
ACCACTTCCC GGCCCCGCAT GAAGAAGAAA CTGAAGATGC TTAACATTGC CAAGAAGATC 
TTACGTATCC AAAAGGAGAC TCCGACCCTA CAACGGAAGG AGCCCCCACC TTCAGTGCTG 
GAGGCTGACC TCACAGAGGG AGACCTGGCC AATTCCCACC TACCCTCTGA GGTACTCTAC 
ATGCTTAAGA ATGTTCGGGT GCTAGGTCAC TTTGAGAAGC CGCTCTTCCT GGAGCTCTGC 
CGGCACATGG TCTTCCAGCG TCTCGGGCAG GGGGACTATG TTTTTCGGCC CGGTCAACCA 
GATGCCAGCA TCTATGTGGT TCAAGATGGG CTGCTGGAGC TCTGCCTACC AGGGCCTGAT 
GGGAAGGAGT GTGTGGTGAA GGAGGTGGTT CCTGGAGACA GCGTCAACAG CCTTCTGAGC 
ATCCTTGATG TCATCACGGG TCACCAGCAT CCCCAGCGGA CTGTGTCAGC CAGGGCTGCA 
CGGGACTCCA CAGTGCTGAG ACTCCCAGTA GAAGCCTTCT CTGCTGTCTT CACCAAGTAC 
CCTGAGAGTT TGGTGCGAGT GGTACAGATC ATCATGGTAA GGCTGCAGAG AGTCACCTTC 
CTGGCGCTTC ACAATTACCT GGGTCTGACC AATGAACTCT TCAGTCATGA GATCCAGCCC 
CTACGCCTTT TCCCCAGCCC CGGCCTCCCG ACCCGAACCA GCCCTGTCCG TGGCTCTAAA 
CGGGTAGTCA GCACCTCAGG TACTGAGGAC ACCTCAAAGG AGACCTCTGG CCGGCCCCTG 
GACTCCATTG GAGCTCCTCT GCCTGGACCT GCAGGGGATC CCGTGAAGCC CACATCCTTA 
GAAGCACCCC CAGCCCCGTT ACTGAGCCGC TGCATCTCCA TGCCAGTAGA CATCTCAGGC 
TTGCAAGGTG GCCCTCGTTC TGATTTTGAC ATGGCCTATG AACGTGGACG GATATCTGTT 
TCTCTTCAAG AAGAGGCATC TGGGGGACCT CAGACAGCAT CCCCTAGGGA GCTCCGGGAG 
CAGCCAGCGG GTGCCTGTGA ATATAGCTAC TGTGAGGATG AGTCAGCCAC AGGCGGATGT 
CCCTTTGGGC CCTACCAGGG CCGCCAGACA AGCAGCATCT TTGAGGCTGC AAAGAGAGAG 
CTAGCCAAGC TGATGCGGAT TGAGGACCCC TCTCTACTGA ACAGCAGAGT CTTGCTACAT 
CATGCCAAAG CCGGCACCAT CATAGCCCGC CAAGGGGACC AGGATGTGAG CCTGCACTTT 
GTGCTCTGGG GCTGCCTGCA CGTGTACCAG CGCATGATTG ACAAGGCTGA GGAAGTGTGC 
CTATTTGTGG CACAGCCAGG GGAGCTGGTG GGGCAACTGG CGGTGCTCAC TGGGGAACCT 
CTCATCTTCA CACTACGTGC CCAGAGAGAC TGCACCTTCC TGCGGATCTC CAAATCCCAC 
TTCTATGAGA TCATGCGTGC ACAACCCAGT GTGGTACTGA GTGCAGCTCA CACGGTGGCT 
GCTAGAATGT CCCCCTTCGT GCGCCAGATG GACTTTGCCA TTGACTGGAC AGCTGTGGAG 
GCTGGTCGTG CGCTCTACAG GCAGGGAGAC CGCTCCGACT GCACCTACAT TGTACTCAAT 
GGACGTCTCC GTAGTGTCAT CCAACGAGGC AGTGGCAAGA AGGAGCTAGT TGGGGAGTAT 
GGTCGAGGGG ATCTCATTGG CGTGGTGGAG GCACTGACCC GGCAGCCACG AGCCACCACT 
GTACACGCAG TGCGAGACAC TGAGCTGGCC AAGCTTCCCG AGGGCACCTT AGGCCACATC 
AAACGTCGGT ACCCACAGGT TGTGACCCGC CTTATTCATC TGCTAAGCCA GAAAATTCTA 
GGCAATCTGC AGCAGTTGCA AGGACCCTTT CCAGGCTCCG GGCTCAGTGT TCCGCAGCAC 
TCGGAACTGA CCAACCCAGC CAGCAATCTG TCTACTGTAG CTATCCTCCC TGTGTGTGCT 
GAGGTGCCCA TGATGGCCTT TACTCTGGAA CTGCAGCATG CTCTGCAAGC TATTGGTCCC 
ACGCTCCTCC TTAACAGTGA CGTCATCCGG GCACTCCTGG GGGCTTCAGC ACTGGATAGC 
ATTCAAGAAT TCCGGCTGTC AGGGTGGTTG GCCCAGCAGG AAGATGCCCA TCGCATTGTG 
CTCTACCAAA CTGACACATC CCTGACGCCT TGGACCGTCC GTTGTCTGCG CCAGGCCGAC 
TGCATCCTCA TTGTGGGCCT GGGGGACCAG GAACCCACTG TTGGCCAGCT GGAGCAAATG 
TTAGAGAACA CTGCTGTCCG TGCCTTGAAG CAACTGGTTT TGCTGCACCG GGAGGAAGGC 
CCTGGTCCCA CGCGCACTGT AGAGTGGCTC AACATGCGCA GCTGGTGCTC AGGGCACCTG 
CACCTGCGCT GTCCTCGCCG CCTCTTCTCA CGGCGCAGCC CAGCGAAACT TCATGAGCTG 
TATGAGAAGG TTTTCTCCAG GCGTGCAGAC CGTCACAGCG ACTTCTCCCG CTTGGCACGA 
GTACTCACAG GAAACACTAT TGCTCTGGTG CTGGGTGGGG GCGGAGCCAG AGGCTGCTCG 
CATATTGGGG TGCTGAAGGC ATTAGAGGAG GCAGGAGTCC CAGTCGACCT TGTGGGAGGC 
ACATCCATAG GTTCCTTCAT TGGGGCCTTG TATGCCGAGG AACGCAGCGC CAGCCGAACT 
AAACAACGAG CCCGGGAGTG GGCCAAGAGC ATGACTTCTG TACTGGAGCC TGTATTGGAC 
CTCACATATC CTGTCACCTC CATGTTTACT GGCTCAGCCT TTAACAGAAG TATCCACCGT 
GTCTTCCAGG ATAAGCAGAT TGAGGACCTG TGGCTGCCTT ACTTCAATGT GACCACAGAC 
ATCACTGCCT CTGCCATGCG CGTCCACAAA GATGGCTCCC TGTGGCGGTA TGTACGTGCC 
AGCATGACGC TCTCGGGCTA CCTACCCCCG CTGTGCGACC CAAAGGATGG GCACCTGCTC 
ATGGATGGTG GCTACATCAA CAACCTGCCA GCGGATATTG CCCGAAGCAT GGGAGCCAAA 
ACGGTCATTG CCATCGACGT TGGAAGCCAG GATGAGACAG ATCTCAGCAC CTATGGGGAC 
AGCCTGTCTG GCTGGTGGTT GCTGTGGAAA AGGCTAAACC CCTGGGCAGA CAAGGTAAAG 
GTTCCAGACA TGGCTGAGAT CCAGTCCCGC TTGGCGTACG TGTCCTGTGT GCGGCAGCTA 
GAAGTCGTCA AGTCCAGCTC CTATTGCGAG TACCTTCGTC CATCCATTGA CTGCTTCAAG 
ACCATGGACT TTGGAAAGTT TGACCAGATC TATGATGTGG GTTACCAGTA TGGGAAGGCT 
GTCTTTGGAG GCTGGACCCG TGGTGAAGTC ATTGAGAAAA TGCTCACAGA CCGGAGATCT 
ACAGACCTTA ATGAGAGTCG CCGTGCAGAT ATACTTGCCT TCCCAAGTTC TGGGTTCACT 
GACTTGGCTG AGATTGTGTC CCGGATCGAG CCACCAACAA GCTACGTCTC TGATGGCTGT 
GCTGATGGGG AGGAGTCGGA TTGCTTGACA GAGTATGAGG AAGATGCAGG ACCAGACTGC 
TCAAGAGATG AAGGGGGCTC CCCTGAGGGT GCCAGCCCTA GCACTGCCTC AGAGGTGGAA 
GAAGAGAAGT CCACACTCCG GCAACGACGC TTTCTGCCTC AGGAGACTCC CAGCTCAGTC 
GCAGATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508893.2
CDS	64..4035
Translation	MEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508830.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508830.3

ORF Insert Sequence:

ATGGAGGCCC CACTGCAAAC CGGAATGGTG CTGGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGCTGGTCA CCGCCGTGCT CATCCTCTTG GTGGTGCGAA GGCTGCGGGT GCAGAAAACT 
CCAGCCCCGG AGGGTCCCCG GTATCGGTTC CGGAAGCGGG ACAAAGTGCT TTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAGTCC ACTTCTTCCC TGGTGGACAC ATCAGTCTCC 
ACCACTTCCC GGCCCCGCAT GAAGAAGAAA CTGAAGATGC TTAACATTGC CAAGAAGATC 
TTACGTATCC AAAAGGAGAC TCCGACCCTA CAACGGAAGG AGCCCCCACC TTCAGTGCTG 
GAGGCTGACC TCACAGAGGG AGACCTGGCC AATTCCCACC TACCCTCTGA GGTACTCTAC 
ATGCTTAAGA ATGTTCGGGT GCTAGGTCAC TTTGAGAAGC CGCTCTTCCT GGAGCTCTGC 
CGGCACATGG TCTTCCAGCG TCTCGGGCAG GGGGACTATG TTTTTCGGCC CGGTCAACCA 
GATGCCAGCA TCTATGTGGT TCAAGATGGG CTGCTGGAGC TCTGCCTACC AGGGCCTGAT 
GGGAAGGAGT GTGTGGTGAA GGAGGTGGTT CCTGGAGACA GCGTCAACAG CCTTCTGAGC 
ATCCTTGATG TCATCACGGG TCACCAGCAT CCCCAGCGGA CTGTGTCAGC CAGGGCTGCA 
CGGGACTCCA CAGTGCTGAG ACTCCCAGTA GAAGCCTTCT CTGCTGTCTT CACCAAGTAC 
CCTGAGAGTT TGGTGCGAGT GGTACAGATC ATCATGGTAA GGCTGCAGAG AGTCACCTTC 
CTGGCGCTTC ACAATTACCT GGGTCTGACC AATGAACTCT TCAGTCATGA GATCCAGCCC 
CTACGCCTTT TCCCCAGCCC CGGCCTCCCG ACCCGAACCA GCCCTGTCCG TGGCTCTAAA 
CGGGTAGTCA GCACCTCAGG TACTGAGGAC ACCTCAAAGG AGACCTCTGG CCGGCCCCTG 
GACTCCATTG GAGCTCCTCT GCCTGGACCT GCAGGGGATC CCGTGAAGCC CACATCCTTA 
GAAGCACCCC CAGCCCCGTT ACTGAGCCGC TGCATCTCCA TGCCAGTAGA CATCTCAGGC 
TTGCAAGGTG GCCCTCGTTC TGATTTTGAC ATGGCCTATG AACGTGGACG GATATCTGTT 
TCTCTTCAAG AAGAGGCATC TGGGGGACCT CAGACAGCAT CCCCTAGGGA GCTCCGGGAG 
CAGCCAGCGG GTGCCTGTGA ATATAGCTAC TGTGAGGATG AGTCAGCCAC AGGCGGATGT 
CCCTTTGGGC CCTACCAGGG CCGCCAGACA AGCAGCATCT TTGAGGCTGC AAAGAGAGAG 
CTAGCCAAGC TGATGCGGAT TGAGGACCCC TCTCTACTGA ACAGCAGAGT CTTGCTACAT 
CATGCCAAAG CCGGCACCAT CATAGCCCGC CAAGGGGACC AGGATGTGAG CCTGCACTTT 
GTGCTCTGGG GCTGCCTGCA CGTGTACCAG CGCATGATTG ACAAGGCTGA GGAAGTGTGC 
CTATTTGTGG CACAGCCAGG GGAGCTGGTG GGGCAACTGG CGGTGCTCAC TGGGGAACCT 
CTCATCTTCA CACTACGTGC CCAGAGAGAC TGCACCTTCC TGCGGATCTC CAAATCCCAC 
TTCTATGAGA TCATGCGTGC ACAACCCAGT GTGGTACTGA GTGCAGCTCA CACGGTGGCT 
GCTAGAATGT CCCCCTTCGT GCGCCAGATG GACTTTGCCA TTGACTGGAC AGCTGTGGAG 
GCTGGTCGTG CGCTCTACAG GCAGGGAGAC CGCTCCGACT GCACCTACAT TGTACTCAAT 
GGACGTCTCC GTAGTGTCAT CCAACGAGGC AGTGGCAAGA AGGAGCTAGT TGGGGAGTAT 
GGTCGAGGGG ATCTCATTGG CGTGGTGGAG GCACTGACCC GGCAGCCACG AGCCACCACT 
GTACACGCAG TGCGAGACAC TGAGCTGGCC AAGCTTCCCG AGGGCACCTT AGGCCACATC 
AAACGTCGGT ACCCACAGGT TGTGACCCGC CTTATTCATC TGCTAAGCCA GAAAATTCTA 
GGCAATCTGC AGCAGTTGCA AGGACCCTTT CCAGGCTCCG GGCTCAGTGT TCCGCAGCAC 
TCGGAACTGA CCAACCCAGC CAGCAATCTG TCTACTGTAG CTATCCTCCC TGTGTGTGCT 
GAGGTGCCCA TGATGGCCTT TACTCTGGAA CTGCAGCATG CTCTGCAAGC TATTGGTCCC 
ACGCTCCTCC TTAACAGTGA CGTCATCCGG GCACTCCTGG GGGCTTCAGC ACTGGATAGC 
ATTCAAGAAT TCCGGCTGTC AGGGTGGTTG GCCCAGCAGG AAGATGCCCA TCGCATTGTG 
CTCTACCAAA CTGACACATC CCTGACGCCT TGGACCGTCC GTTGTCTGCG CCAGGCCGAC 
TGCATCCTCA TTGTGGGCCT GGGGGACCAG GAACCCACTG TTGGCCAGCT GGAGCAAATG 
TTAGAGAACA CTGCTGTCCG TGCCTTGAAG CAACTGGTTT TGCTGCACCG GGAGGAAGGC 
CCTGGTCCCA CGCGCACTGT AGAGTGGCTC AACATGCGCA GCTGGTGCTC AGGGCACCTG 
CACCTGCGCT GTCCTCGCCG CCTCTTCTCA CGGCGCAGCC CAGCGAAACT TCATGAGCTG 
TATGAGAAGG TTTTCTCCAG GCGTGCAGAC CGTCACAGCG ACTTCTCCCG CTTGGCACGA 
GTACTCACAG GAAACACTAT TGCTCTGGTG CTGGGTGGGG GCGGAGCCAG AGGCTGCTCG 
CATATTGGGG TGCTGAAGGC ATTAGAGGAG GCAGGAGTCC CAGTCGACCT TGTGGGAGGC 
ACATCCATAG GTTCCTTCAT TGGGGCCTTG TATGCCGAGG AACGCAGCGC CAGCCGAACT 
AAACAACGAG CCCGGGAGTG GGCCAAGAGC ATGACTTCTG TACTGGAGCC TGTATTGGAC 
CTCACATATC CTGTCACCTC CATGTTTACT GGCTCAGCCT TTAACAGAAG TATCCACCGT 
GTCTTCCAGG ATAAGCAGAT TGAGGACCTG TGGCTGCCTT ACTTCAATGT GACCACAGAC 
ATCACTGCCT CTGCCATGCG CGTCCACAAA GATGGCTCCC TGTGGCGGTA TGTACGTGCC 
AGCATGACGC TCTCGGGCTA CCTACCCCCG CTGTGCGACC CAAAGGATGG GCACCTGCTC 
ATGGATGGTG GCTACATCAA CAACCTGCCA GCGGATATTG CCCGAAGCAT GGGAGCCAAA 
ACGGTCATTG CCATCGACGT TGGAAGCCAG GATGAGACAG ATCTCAGCAC CTATGGGGAC 
AGCCTGTCTG GCTGGTGGTT GCTGTGGAAA AGGCTAAACC CCTGGGCAGA CAAGGTAAAG 
GTTCCAGACA TGGCTGAGAT CCAGTCCCGC TTGGCGTACG TGTCCTGTGT GCGGCAGCTA 
GAAGTCGTCA AGTCCAGCTC CTATTGCGAG TACCTTCGTC CATCCATTGA CTGCTTCAAG 
ACCATGGACT TTGGAAAGTT TGACCAGATC TATGATGTGG GTTACCAGTA TGGGAAGGCT 
GTCTTTGGAG GCTGGACCCG TGGTGAAGTC ATTGAGAAAA TGCTCACAGA CCGGAGATCT 
ACAGACCTTA ATGAGAGTCG CCGTGCAGAT ATACTTGCCT TCCCAAGTTC TGGGTTCACT 
GACTTGGCTG AGATTGTGTC CCGGATCGAG CCACCAACAA GCTACGTCTC TGATGGCTGT 
GCTGATGGGG AGGAGTCGGA TTGCTTGACA GAGTATGAGG AAGATGCAGG ACCAGACTGC 
TCAAGAGATG AAGGGGGCTC CCCTGAGGGT GCCAGCCCTA GCACTGCCTC AGAGGTGGAA 
GAAGAGAAGT CCACACTCCG GCAACGACGC TTTCTGCCTC AGGAGACTCC CAGCTCAGTC 
GCAGATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( NM_001122818.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001116290.2 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 1, mRNA.

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CloneID	OMu05750
Clone ID Related Accession (Same CDS sequence)	NM_001122818.2 , XM_006508831.3 , XM_006508831.2 , XM_006508831.4
Accession Version	NM_001122818.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY322839.1 and BC054789.1. On Jun 12, 2012 this sequence version replaced NM_001122818.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054789.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGGAGCTC 
CGGGAGCAGC CAGCGGGTGC CTGTGAATAT AGCTACTGTG AGGATGAGTC AGCCACAGGC 
GGATGTCCCT TTGGGCCCTA CCAGGGCCGC CAGACAAGCA GCATCTTTGA GGCTGCAAAG 
AGAGAGCTAG CCAAGCTGAT GCGGATTGAG GACCCCTCTC TACTGAACAG CAGAGTCTTG 
CTACATCATG CCAAAGCCGG CACCATCATA GCCCGCCAAG GGGACCAGGA TGTGAGCCTG 
CACTTTGTGC TCTGGGGCTG CCTGCACGTG TACCAGCGCA TGATTGACAA GGCTGAGGAA 
GTGTGCCTAT TTGTGGCACA GCCAGGGGAG CTGGTGGGGC AACTGGCGGT GCTCACTGGG 
GAACCTCTCA TCTTCACACT ACGTGCCCAG AGAGACTGCA CCTTCCTGCG GATCTCCAAA 
TCCCACTTCT ATGAGATCAT GCGTGCACAA CCCAGTGTGG TACTGAGTGC AGCTCACACG 
GTGGCTGCTA GAATGTCCCC CTTCGTGCGC CAGATGGACT TTGCCATTGA CTGGACAGCT 
GTGGAGGCTG GTCGTGCGCT CTACAGGCAG GGAGACCGCT CCGACTGCAC CTACATTGTA 
CTCAATGGAC GTCTCCGTAG TGTCATCCAA CGAGGCAGTG GCAAGAAGGA GCTAGTTGGG 
GAGTATGGTC GAGGGGATCT CATTGGCGTG GTGGAGGCAC TGACCCGGCA GCCACGAGCC 
ACCACTGTAC ACGCAGTGCG AGACACTGAG CTGGCCAAGC TTCCCGAGGG CACCTTAGGC 
CACATCAAAC GTCGGTACCC ACAGGTTGTG ACCCGCCTTA TTCATCTGCT AAGCCAGAAA 
ATTCTAGGCA ATCTGCAGCA GTTGCAAGGA CCCTTTCCAG GCTCCGGGCT CAGTGTTCCG 
CAGCACTCGG AACTGACCAA CCCAGCCAGC AATCTGTCTA CTGTAGCTAT CCTCCCTGTG 
TGTGCTGAGG TGCCCATGAT GGCCTTTACT CTGGAACTGC AGCATGCTCT GCAAGCTATT 
GGTCCCACGC TCCTCCTTAA CAGTGACGTC ATCCGGGCAC TCCTGGGGGC TTCAGCACTG 
GATAGCATTC AAGAATTCCG GCTGTCAGGG TGGTTGGCCC AGCAGGAAGA TGCCCATCGC 
ATTGTGCTCT ACCAAACTGA CACATCCCTG ACGCCTTGGA CCGTCCGTTG TCTGCGCCAG 
GCCGACTGCA TCCTCATTGT GGGCCTGGGG GACCAGGAAC CCACTGTTGG CCAGCTGGAG 
CAAATGTTAG AGAACACTGC TGTCCGTGCC TTGAAGCAAC TGGTTTTGCT GCACCGGGAG 
GAAGGCCCTG GTCCCACGCG CACTGTAGAG TGGCTCAACA TGCGCAGCTG GTGCTCAGGG 
CACCTGCACC TGCGCTGTCC TCGCCGCCTC TTCTCACGGC GCAGCCCAGC GAAACTTCAT 
GAGCTGTATG AGAAGGTTTT CTCCAGGCGT GCAGACCGTC ACAGCGACTT CTCCCGCTTG 
GCACGAGTAC TCACAGGAAA CACTATTGCT CTGGTGCTGG GTGGGGGCGG AGCCAGAGGC 
TGCTCGCATA TTGGGGTGCT GAAGGCATTA GAGGAGGCAG GAGTCCCAGT CGACCTTGTG 
GGAGGCACAT CCATAGGTTC CTTCATTGGG GCCTTGTATG CCGAGGAACG CAGCGCCAGC 
CGAACTAAAC AACGAGCCCG GGAGTGGGCC AAGAGCATGA CTTCTGTACT GGAGCCTGTA 
TTGGACCTCA CATATCCTGT CACCTCCATG TTTACTGGCT CAGCCTTTAA CAGAAGTATC 
CACCGTGTCT TCCAGGATAA GCAGATTGAG GACCTGTGGC TGCCTTACTT CAATGTGACC 
ACAGACATCA CTGCCTCTGC CATGCGCGTC CACAAAGATG GCTCCCTGTG GCGGTATGTA 
CGTGCCAGCA TGACGCTCTC GGGCTACCTA CCCCCGCTGT GCGACCCAAA GGATGGGCAC 
CTGCTCATGG ATGGTGGCTA CATCAACAAC CTGCCAGCGG ATATTGCCCG AAGCATGGGA 
GCCAAAACGG TCATTGCCAT CGACGTTGGA AGCCAGGATG AGACAGATCT CAGCACCTAT 
GGGGACAGCC TGTCTGGCTG GTGGTTGCTG TGGAAAAGGC TAAACCCCTG GGCAGACAAG 
GTAAAGGTTC CAGACATGGC TGAGATCCAG TCCCGCTTGG CGTACGTGTC CTGTGTGCGG 
CAGCTAGAAG TCGTCAAGTC CAGCTCCTAT TGCGAGTACC TTCGTCCATC CATTGACTGC 
TTCAAGACCA TGGACTTTGG AAAGTTTGAC CAGATCTATG ATGTGGGTTA CCAGTATGGG 
AAGGCTGTCT TTGGAGGCTG GACCCGTGGT GAAGTCATTG AGAAAATGCT CACAGACCGG 
AGATCTACAG ACCTTAATGA GAGTCGCCGT GCAGATATAC TTGCCTTCCC AAGTTCTGGG 
TTCACTGACT TGGCTGAGAT TGTGTCCCGG ATCGAGCCAC CAACAAGCTA CGTCTCTGAT 
GGCTGTGCTG ATGGGGAGGA GTCGGATTGC TTGACAGAGT ATGAGGAAGA TGCAGGACCA 
GACTGCTCAA GAGATGAAGG GGGCTCCCCT GAGGGTGCCA GCCCTAGCAC TGCCTCAGAG 
GTGGAAGAAG AGAAGTCCAC ACTCCGGCAA CGACGCTTTC TGCCTCAGGA GACTCCCAGC 
TCAGTCGCAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116290.2
CDS	209..4246
Translation	MEESLQPKDCRLQDLLPSPKQSMEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	NM_001122818.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122818.2

ORF Insert Sequence:

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGGAGCTC 
CGGGAGCAGC CAGCGGGTGC CTGTGAATAT AGCTACTGTG AGGATGAGTC AGCCACAGGC 
GGATGTCCCT TTGGGCCCTA CCAGGGCCGC CAGACAAGCA GCATCTTTGA GGCTGCAAAG 
AGAGAGCTAG CCAAGCTGAT GCGGATTGAG GACCCCTCTC TACTGAACAG CAGAGTCTTG 
CTACATCATG CCAAAGCCGG CACCATCATA GCCCGCCAAG GGGACCAGGA TGTGAGCCTG 
CACTTTGTGC TCTGGGGCTG CCTGCACGTG TACCAGCGCA TGATTGACAA GGCTGAGGAA 
GTGTGCCTAT TTGTGGCACA GCCAGGGGAG CTGGTGGGGC AACTGGCGGT GCTCACTGGG 
GAACCTCTCA TCTTCACACT ACGTGCCCAG AGAGACTGCA CCTTCCTGCG GATCTCCAAA 
TCCCACTTCT ATGAGATCAT GCGTGCACAA CCCAGTGTGG TACTGAGTGC AGCTCACACG 
GTGGCTGCTA GAATGTCCCC CTTCGTGCGC CAGATGGACT TTGCCATTGA CTGGACAGCT 
GTGGAGGCTG GTCGTGCGCT CTACAGGCAG GGAGACCGCT CCGACTGCAC CTACATTGTA 
CTCAATGGAC GTCTCCGTAG TGTCATCCAA CGAGGCAGTG GCAAGAAGGA GCTAGTTGGG 
GAGTATGGTC GAGGGGATCT CATTGGCGTG GTGGAGGCAC TGACCCGGCA GCCACGAGCC 
ACCACTGTAC ACGCAGTGCG AGACACTGAG CTGGCCAAGC TTCCCGAGGG CACCTTAGGC 
CACATCAAAC GTCGGTACCC ACAGGTTGTG ACCCGCCTTA TTCATCTGCT AAGCCAGAAA 
ATTCTAGGCA ATCTGCAGCA GTTGCAAGGA CCCTTTCCAG GCTCCGGGCT CAGTGTTCCG 
CAGCACTCGG AACTGACCAA CCCAGCCAGC AATCTGTCTA CTGTAGCTAT CCTCCCTGTG 
TGTGCTGAGG TGCCCATGAT GGCCTTTACT CTGGAACTGC AGCATGCTCT GCAAGCTATT 
GGTCCCACGC TCCTCCTTAA CAGTGACGTC ATCCGGGCAC TCCTGGGGGC TTCAGCACTG 
GATAGCATTC AAGAATTCCG GCTGTCAGGG TGGTTGGCCC AGCAGGAAGA TGCCCATCGC 
ATTGTGCTCT ACCAAACTGA CACATCCCTG ACGCCTTGGA CCGTCCGTTG TCTGCGCCAG 
GCCGACTGCA TCCTCATTGT GGGCCTGGGG GACCAGGAAC CCACTGTTGG CCAGCTGGAG 
CAAATGTTAG AGAACACTGC TGTCCGTGCC TTGAAGCAAC TGGTTTTGCT GCACCGGGAG 
GAAGGCCCTG GTCCCACGCG CACTGTAGAG TGGCTCAACA TGCGCAGCTG GTGCTCAGGG 
CACCTGCACC TGCGCTGTCC TCGCCGCCTC TTCTCACGGC GCAGCCCAGC GAAACTTCAT 
GAGCTGTATG AGAAGGTTTT CTCCAGGCGT GCAGACCGTC ACAGCGACTT CTCCCGCTTG 
GCACGAGTAC TCACAGGAAA CACTATTGCT CTGGTGCTGG GTGGGGGCGG AGCCAGAGGC 
TGCTCGCATA TTGGGGTGCT GAAGGCATTA GAGGAGGCAG GAGTCCCAGT CGACCTTGTG 
GGAGGCACAT CCATAGGTTC CTTCATTGGG GCCTTGTATG CCGAGGAACG CAGCGCCAGC 
CGAACTAAAC AACGAGCCCG GGAGTGGGCC AAGAGCATGA CTTCTGTACT GGAGCCTGTA 
TTGGACCTCA CATATCCTGT CACCTCCATG TTTACTGGCT CAGCCTTTAA CAGAAGTATC 
CACCGTGTCT TCCAGGATAA GCAGATTGAG GACCTGTGGC TGCCTTACTT CAATGTGACC 
ACAGACATCA CTGCCTCTGC CATGCGCGTC CACAAAGATG GCTCCCTGTG GCGGTATGTA 
CGTGCCAGCA TGACGCTCTC GGGCTACCTA CCCCCGCTGT GCGACCCAAA GGATGGGCAC 
CTGCTCATGG ATGGTGGCTA CATCAACAAC CTGCCAGCGG ATATTGCCCG AAGCATGGGA 
GCCAAAACGG TCATTGCCAT CGACGTTGGA AGCCAGGATG AGACAGATCT CAGCACCTAT 
GGGGACAGCC TGTCTGGCTG GTGGTTGCTG TGGAAAAGGC TAAACCCCTG GGCAGACAAG 
GTAAAGGTTC CAGACATGGC TGAGATCCAG TCCCGCTTGG CGTACGTGTC CTGTGTGCGG 
CAGCTAGAAG TCGTCAAGTC CAGCTCCTAT TGCGAGTACC TTCGTCCATC CATTGACTGC 
TTCAAGACCA TGGACTTTGG AAAGTTTGAC CAGATCTATG ATGTGGGTTA CCAGTATGGG 
AAGGCTGTCT TTGGAGGCTG GACCCGTGGT GAAGTCATTG AGAAAATGCT CACAGACCGG 
AGATCTACAG ACCTTAATGA GAGTCGCCGT GCAGATATAC TTGCCTTCCC AAGTTCTGGG 
TTCACTGACT TGGCTGAGAT TGTGTCCCGG ATCGAGCCAC CAACAAGCTA CGTCTCTGAT 
GGCTGTGCTG ATGGGGAGGA GTCGGATTGC TTGACAGAGT ATGAGGAAGA TGCAGGACCA 
GACTGCTCAA GAGATGAAGG GGGCTCCCCT GAGGGTGCCA GCCCTAGCAC TGCCTCAGAG 
GTGGAAGAAG AGAAGTCCAC ACTCCGGCAA CGACGCTTTC TGCCTCAGGA GACTCCCAGC 
TCAGTCGCAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508831.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508894.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X10, mRNA.

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CloneID	OMu05750
Clone ID Related Accession (Same CDS sequence)	NM_001122818.2 , XM_006508831.3 , XM_006508831.2 , XM_006508831.4
Accession Version	XM_006508831.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGGAGCTC 
CGGGAGCAGC CAGCGGGTGC CTGTGAATAT AGCTACTGTG AGGATGAGTC AGCCACAGGC 
GGATGTCCCT TTGGGCCCTA CCAGGGCCGC CAGACAAGCA GCATCTTTGA GGCTGCAAAG 
AGAGAGCTAG CCAAGCTGAT GCGGATTGAG GACCCCTCTC TACTGAACAG CAGAGTCTTG 
CTACATCATG CCAAAGCCGG CACCATCATA GCCCGCCAAG GGGACCAGGA TGTGAGCCTG 
CACTTTGTGC TCTGGGGCTG CCTGCACGTG TACCAGCGCA TGATTGACAA GGCTGAGGAA 
GTGTGCCTAT TTGTGGCACA GCCAGGGGAG CTGGTGGGGC AACTGGCGGT GCTCACTGGG 
GAACCTCTCA TCTTCACACT ACGTGCCCAG AGAGACTGCA CCTTCCTGCG GATCTCCAAA 
TCCCACTTCT ATGAGATCAT GCGTGCACAA CCCAGTGTGG TACTGAGTGC AGCTCACACG 
GTGGCTGCTA GAATGTCCCC CTTCGTGCGC CAGATGGACT TTGCCATTGA CTGGACAGCT 
GTGGAGGCTG GTCGTGCGCT CTACAGGCAG GGAGACCGCT CCGACTGCAC CTACATTGTA 
CTCAATGGAC GTCTCCGTAG TGTCATCCAA CGAGGCAGTG GCAAGAAGGA GCTAGTTGGG 
GAGTATGGTC GAGGGGATCT CATTGGCGTG GTGGAGGCAC TGACCCGGCA GCCACGAGCC 
ACCACTGTAC ACGCAGTGCG AGACACTGAG CTGGCCAAGC TTCCCGAGGG CACCTTAGGC 
CACATCAAAC GTCGGTACCC ACAGGTTGTG ACCCGCCTTA TTCATCTGCT AAGCCAGAAA 
ATTCTAGGCA ATCTGCAGCA GTTGCAAGGA CCCTTTCCAG GCTCCGGGCT CAGTGTTCCG 
CAGCACTCGG AACTGACCAA CCCAGCCAGC AATCTGTCTA CTGTAGCTAT CCTCCCTGTG 
TGTGCTGAGG TGCCCATGAT GGCCTTTACT CTGGAACTGC AGCATGCTCT GCAAGCTATT 
GGTCCCACGC TCCTCCTTAA CAGTGACGTC ATCCGGGCAC TCCTGGGGGC TTCAGCACTG 
GATAGCATTC AAGAATTCCG GCTGTCAGGG TGGTTGGCCC AGCAGGAAGA TGCCCATCGC 
ATTGTGCTCT ACCAAACTGA CACATCCCTG ACGCCTTGGA CCGTCCGTTG TCTGCGCCAG 
GCCGACTGCA TCCTCATTGT GGGCCTGGGG GACCAGGAAC CCACTGTTGG CCAGCTGGAG 
CAAATGTTAG AGAACACTGC TGTCCGTGCC TTGAAGCAAC TGGTTTTGCT GCACCGGGAG 
GAAGGCCCTG GTCCCACGCG CACTGTAGAG TGGCTCAACA TGCGCAGCTG GTGCTCAGGG 
CACCTGCACC TGCGCTGTCC TCGCCGCCTC TTCTCACGGC GCAGCCCAGC GAAACTTCAT 
GAGCTGTATG AGAAGGTTTT CTCCAGGCGT GCAGACCGTC ACAGCGACTT CTCCCGCTTG 
GCACGAGTAC TCACAGGAAA CACTATTGCT CTGGTGCTGG GTGGGGGCGG AGCCAGAGGC 
TGCTCGCATA TTGGGGTGCT GAAGGCATTA GAGGAGGCAG GAGTCCCAGT CGACCTTGTG 
GGAGGCACAT CCATAGGTTC CTTCATTGGG GCCTTGTATG CCGAGGAACG CAGCGCCAGC 
CGAACTAAAC AACGAGCCCG GGAGTGGGCC AAGAGCATGA CTTCTGTACT GGAGCCTGTA 
TTGGACCTCA CATATCCTGT CACCTCCATG TTTACTGGCT CAGCCTTTAA CAGAAGTATC 
CACCGTGTCT TCCAGGATAA GCAGATTGAG GACCTGTGGC TGCCTTACTT CAATGTGACC 
ACAGACATCA CTGCCTCTGC CATGCGCGTC CACAAAGATG GCTCCCTGTG GCGGTATGTA 
CGTGCCAGCA TGACGCTCTC GGGCTACCTA CCCCCGCTGT GCGACCCAAA GGATGGGCAC 
CTGCTCATGG ATGGTGGCTA CATCAACAAC CTGCCAGCGG ATATTGCCCG AAGCATGGGA 
GCCAAAACGG TCATTGCCAT CGACGTTGGA AGCCAGGATG AGACAGATCT CAGCACCTAT 
GGGGACAGCC TGTCTGGCTG GTGGTTGCTG TGGAAAAGGC TAAACCCCTG GGCAGACAAG 
GTAAAGGTTC CAGACATGGC TGAGATCCAG TCCCGCTTGG CGTACGTGTC CTGTGTGCGG 
CAGCTAGAAG TCGTCAAGTC CAGCTCCTAT TGCGAGTACC TTCGTCCATC CATTGACTGC 
TTCAAGACCA TGGACTTTGG AAAGTTTGAC CAGATCTATG ATGTGGGTTA CCAGTATGGG 
AAGGCTGTCT TTGGAGGCTG GACCCGTGGT GAAGTCATTG AGAAAATGCT CACAGACCGG 
AGATCTACAG ACCTTAATGA GAGTCGCCGT GCAGATATAC TTGCCTTCCC AAGTTCTGGG 
TTCACTGACT TGGCTGAGAT TGTGTCCCGG ATCGAGCCAC CAACAAGCTA CGTCTCTGAT 
GGCTGTGCTG ATGGGGAGGA GTCGGATTGC TTGACAGAGT ATGAGGAAGA TGCAGGACCA 
GACTGCTCAA GAGATGAAGG GGGCTCCCCT GAGGGTGCCA GCCCTAGCAC TGCCTCAGAG 
GTGGAAGAAG AGAAGTCCAC ACTCCGGCAA CGACGCTTTC TGCCTCAGGA GACTCCCAGC 
TCAGTCGCAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508894.1
CDS	258..4295
Translation	MEESLQPKDCRLQDLLPSPKQSMEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508831.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508831.3

ORF Insert Sequence:

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGGAGCTC 
CGGGAGCAGC CAGCGGGTGC CTGTGAATAT AGCTACTGTG AGGATGAGTC AGCCACAGGC 
GGATGTCCCT TTGGGCCCTA CCAGGGCCGC CAGACAAGCA GCATCTTTGA GGCTGCAAAG 
AGAGAGCTAG CCAAGCTGAT GCGGATTGAG GACCCCTCTC TACTGAACAG CAGAGTCTTG 
CTACATCATG CCAAAGCCGG CACCATCATA GCCCGCCAAG GGGACCAGGA TGTGAGCCTG 
CACTTTGTGC TCTGGGGCTG CCTGCACGTG TACCAGCGCA TGATTGACAA GGCTGAGGAA 
GTGTGCCTAT TTGTGGCACA GCCAGGGGAG CTGGTGGGGC AACTGGCGGT GCTCACTGGG 
GAACCTCTCA TCTTCACACT ACGTGCCCAG AGAGACTGCA CCTTCCTGCG GATCTCCAAA 
TCCCACTTCT ATGAGATCAT GCGTGCACAA CCCAGTGTGG TACTGAGTGC AGCTCACACG 
GTGGCTGCTA GAATGTCCCC CTTCGTGCGC CAGATGGACT TTGCCATTGA CTGGACAGCT 
GTGGAGGCTG GTCGTGCGCT CTACAGGCAG GGAGACCGCT CCGACTGCAC CTACATTGTA 
CTCAATGGAC GTCTCCGTAG TGTCATCCAA CGAGGCAGTG GCAAGAAGGA GCTAGTTGGG 
GAGTATGGTC GAGGGGATCT CATTGGCGTG GTGGAGGCAC TGACCCGGCA GCCACGAGCC 
ACCACTGTAC ACGCAGTGCG AGACACTGAG CTGGCCAAGC TTCCCGAGGG CACCTTAGGC 
CACATCAAAC GTCGGTACCC ACAGGTTGTG ACCCGCCTTA TTCATCTGCT AAGCCAGAAA 
ATTCTAGGCA ATCTGCAGCA GTTGCAAGGA CCCTTTCCAG GCTCCGGGCT CAGTGTTCCG 
CAGCACTCGG AACTGACCAA CCCAGCCAGC AATCTGTCTA CTGTAGCTAT CCTCCCTGTG 
TGTGCTGAGG TGCCCATGAT GGCCTTTACT CTGGAACTGC AGCATGCTCT GCAAGCTATT 
GGTCCCACGC TCCTCCTTAA CAGTGACGTC ATCCGGGCAC TCCTGGGGGC TTCAGCACTG 
GATAGCATTC AAGAATTCCG GCTGTCAGGG TGGTTGGCCC AGCAGGAAGA TGCCCATCGC 
ATTGTGCTCT ACCAAACTGA CACATCCCTG ACGCCTTGGA CCGTCCGTTG TCTGCGCCAG 
GCCGACTGCA TCCTCATTGT GGGCCTGGGG GACCAGGAAC CCACTGTTGG CCAGCTGGAG 
CAAATGTTAG AGAACACTGC TGTCCGTGCC TTGAAGCAAC TGGTTTTGCT GCACCGGGAG 
GAAGGCCCTG GTCCCACGCG CACTGTAGAG TGGCTCAACA TGCGCAGCTG GTGCTCAGGG 
CACCTGCACC TGCGCTGTCC TCGCCGCCTC TTCTCACGGC GCAGCCCAGC GAAACTTCAT 
GAGCTGTATG AGAAGGTTTT CTCCAGGCGT GCAGACCGTC ACAGCGACTT CTCCCGCTTG 
GCACGAGTAC TCACAGGAAA CACTATTGCT CTGGTGCTGG GTGGGGGCGG AGCCAGAGGC 
TGCTCGCATA TTGGGGTGCT GAAGGCATTA GAGGAGGCAG GAGTCCCAGT CGACCTTGTG 
GGAGGCACAT CCATAGGTTC CTTCATTGGG GCCTTGTATG CCGAGGAACG CAGCGCCAGC 
CGAACTAAAC AACGAGCCCG GGAGTGGGCC AAGAGCATGA CTTCTGTACT GGAGCCTGTA 
TTGGACCTCA CATATCCTGT CACCTCCATG TTTACTGGCT CAGCCTTTAA CAGAAGTATC 
CACCGTGTCT TCCAGGATAA GCAGATTGAG GACCTGTGGC TGCCTTACTT CAATGTGACC 
ACAGACATCA CTGCCTCTGC CATGCGCGTC CACAAAGATG GCTCCCTGTG GCGGTATGTA 
CGTGCCAGCA TGACGCTCTC GGGCTACCTA CCCCCGCTGT GCGACCCAAA GGATGGGCAC 
CTGCTCATGG ATGGTGGCTA CATCAACAAC CTGCCAGCGG ATATTGCCCG AAGCATGGGA 
GCCAAAACGG TCATTGCCAT CGACGTTGGA AGCCAGGATG AGACAGATCT CAGCACCTAT 
GGGGACAGCC TGTCTGGCTG GTGGTTGCTG TGGAAAAGGC TAAACCCCTG GGCAGACAAG 
GTAAAGGTTC CAGACATGGC TGAGATCCAG TCCCGCTTGG CGTACGTGTC CTGTGTGCGG 
CAGCTAGAAG TCGTCAAGTC CAGCTCCTAT TGCGAGTACC TTCGTCCATC CATTGACTGC 
TTCAAGACCA TGGACTTTGG AAAGTTTGAC CAGATCTATG ATGTGGGTTA CCAGTATGGG 
AAGGCTGTCT TTGGAGGCTG GACCCGTGGT GAAGTCATTG AGAAAATGCT CACAGACCGG 
AGATCTACAG ACCTTAATGA GAGTCGCCGT GCAGATATAC TTGCCTTCCC AAGTTCTGGG 
TTCACTGACT TGGCTGAGAT TGTGTCCCGG ATCGAGCCAC CAACAAGCTA CGTCTCTGAT 
GGCTGTGCTG ATGGGGAGGA GTCGGATTGC TTGACAGAGT ATGAGGAAGA TGCAGGACCA 
GACTGCTCAA GAGATGAAGG GGGCTCCCCT GAGGGTGCCA GCCCTAGCAC TGCCTCAGAG 
GTGGAAGAAG AGAAGTCCAC ACTCCGGCAA CGACGCTTTC TGCCTCAGGA GACTCCCAGC 
TCAGTCGCAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508831.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508894.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu05750
Clone ID Related Accession (Same CDS sequence)	NM_001122818.2 , XM_006508831.3 , XM_006508831.2 , XM_006508831.4
Accession Version	XM_006508831.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	neuropathy target esterase isoform X1
Comment

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGGAGCTC 
CGGGAGCAGC CAGCGGGTGC CTGTGAATAT AGCTACTGTG AGGATGAGTC AGCCACAGGC 
GGATGTCCCT TTGGGCCCTA CCAGGGCCGC CAGACAAGCA GCATCTTTGA GGCTGCAAAG 
AGAGAGCTAG CCAAGCTGAT GCGGATTGAG GACCCCTCTC TACTGAACAG CAGAGTCTTG 
CTACATCATG CCAAAGCCGG CACCATCATA GCCCGCCAAG GGGACCAGGA TGTGAGCCTG 
CACTTTGTGC TCTGGGGCTG CCTGCACGTG TACCAGCGCA TGATTGACAA GGCTGAGGAA 
GTGTGCCTAT TTGTGGCACA GCCAGGGGAG CTGGTGGGGC AACTGGCGGT GCTCACTGGG 
GAACCTCTCA TCTTCACACT ACGTGCCCAG AGAGACTGCA CCTTCCTGCG GATCTCCAAA 
TCCCACTTCT ATGAGATCAT GCGTGCACAA CCCAGTGTGG TACTGAGTGC AGCTCACACG 
GTGGCTGCTA GAATGTCCCC CTTCGTGCGC CAGATGGACT TTGCCATTGA CTGGACAGCT 
GTGGAGGCTG GTCGTGCGCT CTACAGGCAG GGAGACCGCT CCGACTGCAC CTACATTGTA 
CTCAATGGAC GTCTCCGTAG TGTCATCCAA CGAGGCAGTG GCAAGAAGGA GCTAGTTGGG 
GAGTATGGTC GAGGGGATCT CATTGGCGTG GTGGAGGCAC TGACCCGGCA GCCACGAGCC 
ACCACTGTAC ACGCAGTGCG AGACACTGAG CTGGCCAAGC TTCCCGAGGG CACCTTAGGC 
CACATCAAAC GTCGGTACCC ACAGGTTGTG ACCCGCCTTA TTCATCTGCT AAGCCAGAAA 
ATTCTAGGCA ATCTGCAGCA GTTGCAAGGA CCCTTTCCAG GCTCCGGGCT CAGTGTTCCG 
CAGCACTCGG AACTGACCAA CCCAGCCAGC AATCTGTCTA CTGTAGCTAT CCTCCCTGTG 
TGTGCTGAGG TGCCCATGAT GGCCTTTACT CTGGAACTGC AGCATGCTCT GCAAGCTATT 
GGTCCCACGC TCCTCCTTAA CAGTGACGTC ATCCGGGCAC TCCTGGGGGC TTCAGCACTG 
GATAGCATTC AAGAATTCCG GCTGTCAGGG TGGTTGGCCC AGCAGGAAGA TGCCCATCGC 
ATTGTGCTCT ACCAAACTGA CACATCCCTG ACGCCTTGGA CCGTCCGTTG TCTGCGCCAG 
GCCGACTGCA TCCTCATTGT GGGCCTGGGG GACCAGGAAC CCACTGTTGG CCAGCTGGAG 
CAAATGTTAG AGAACACTGC TGTCCGTGCC TTGAAGCAAC TGGTTTTGCT GCACCGGGAG 
GAAGGCCCTG GTCCCACGCG CACTGTAGAG TGGCTCAACA TGCGCAGCTG GTGCTCAGGG 
CACCTGCACC TGCGCTGTCC TCGCCGCCTC TTCTCACGGC GCAGCCCAGC GAAACTTCAT 
GAGCTGTATG AGAAGGTTTT CTCCAGGCGT GCAGACCGTC ACAGCGACTT CTCCCGCTTG 
GCACGAGTAC TCACAGGAAA CACTATTGCT CTGGTGCTGG GTGGGGGCGG AGCCAGAGGC 
TGCTCGCATA TTGGGGTGCT GAAGGCATTA GAGGAGGCAG GAGTCCCAGT CGACCTTGTG 
GGAGGCACAT CCATAGGTTC CTTCATTGGG GCCTTGTATG CCGAGGAACG CAGCGCCAGC 
CGAACTAAAC AACGAGCCCG GGAGTGGGCC AAGAGCATGA CTTCTGTACT GGAGCCTGTA 
TTGGACCTCA CATATCCTGT CACCTCCATG TTTACTGGCT CAGCCTTTAA CAGAAGTATC 
CACCGTGTCT TCCAGGATAA GCAGATTGAG GACCTGTGGC TGCCTTACTT CAATGTGACC 
ACAGACATCA CTGCCTCTGC CATGCGCGTC CACAAAGATG GCTCCCTGTG GCGGTATGTA 
CGTGCCAGCA TGACGCTCTC GGGCTACCTA CCCCCGCTGT GCGACCCAAA GGATGGGCAC 
CTGCTCATGG ATGGTGGCTA CATCAACAAC CTGCCAGCGG ATATTGCCCG AAGCATGGGA 
GCCAAAACGG TCATTGCCAT CGACGTTGGA AGCCAGGATG AGACAGATCT CAGCACCTAT 
GGGGACAGCC TGTCTGGCTG GTGGTTGCTG TGGAAAAGGC TAAACCCCTG GGCAGACAAG 
GTAAAGGTTC CAGACATGGC TGAGATCCAG TCCCGCTTGG CGTACGTGTC CTGTGTGCGG 
CAGCTAGAAG TCGTCAAGTC CAGCTCCTAT TGCGAGTACC TTCGTCCATC CATTGACTGC 
TTCAAGACCA TGGACTTTGG AAAGTTTGAC CAGATCTATG ATGTGGGTTA CCAGTATGGG 
AAGGCTGTCT TTGGAGGCTG GACCCGTGGT GAAGTCATTG AGAAAATGCT CACAGACCGG 
AGATCTACAG ACCTTAATGA GAGTCGCCGT GCAGATATAC TTGCCTTCCC AAGTTCTGGG 
TTCACTGACT TGGCTGAGAT TGTGTCCCGG ATCGAGCCAC CAACAAGCTA CGTCTCTGAT 
GGCTGTGCTG ATGGGGAGGA GTCGGATTGC TTGACAGAGT ATGAGGAAGA TGCAGGACCA 
GACTGCTCAA GAGATGAAGG GGGCTCCCCT GAGGGTGCCA GCCCTAGCAC TGCCTCAGAG 
GTGGAAGAAG AGAAGTCCAC ACTCCGGCAA CGACGCTTTC TGCCTCAGGA GACTCCCAGC 
TCAGTCGCAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006508894.1

Translation

MEESLQPKDCRLQDLLPSPKQSMEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508831.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508831.2

ORF Insert Sequence:

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGGAGCTC 
CGGGAGCAGC CAGCGGGTGC CTGTGAATAT AGCTACTGTG AGGATGAGTC AGCCACAGGC 
GGATGTCCCT TTGGGCCCTA CCAGGGCCGC CAGACAAGCA GCATCTTTGA GGCTGCAAAG 
AGAGAGCTAG CCAAGCTGAT GCGGATTGAG GACCCCTCTC TACTGAACAG CAGAGTCTTG 
CTACATCATG CCAAAGCCGG CACCATCATA GCCCGCCAAG GGGACCAGGA TGTGAGCCTG 
CACTTTGTGC TCTGGGGCTG CCTGCACGTG TACCAGCGCA TGATTGACAA GGCTGAGGAA 
GTGTGCCTAT TTGTGGCACA GCCAGGGGAG CTGGTGGGGC AACTGGCGGT GCTCACTGGG 
GAACCTCTCA TCTTCACACT ACGTGCCCAG AGAGACTGCA CCTTCCTGCG GATCTCCAAA 
TCCCACTTCT ATGAGATCAT GCGTGCACAA CCCAGTGTGG TACTGAGTGC AGCTCACACG 
GTGGCTGCTA GAATGTCCCC CTTCGTGCGC CAGATGGACT TTGCCATTGA CTGGACAGCT 
GTGGAGGCTG GTCGTGCGCT CTACAGGCAG GGAGACCGCT CCGACTGCAC CTACATTGTA 
CTCAATGGAC GTCTCCGTAG TGTCATCCAA CGAGGCAGTG GCAAGAAGGA GCTAGTTGGG 
GAGTATGGTC GAGGGGATCT CATTGGCGTG GTGGAGGCAC TGACCCGGCA GCCACGAGCC 
ACCACTGTAC ACGCAGTGCG AGACACTGAG CTGGCCAAGC TTCCCGAGGG CACCTTAGGC 
CACATCAAAC GTCGGTACCC ACAGGTTGTG ACCCGCCTTA TTCATCTGCT AAGCCAGAAA 
ATTCTAGGCA ATCTGCAGCA GTTGCAAGGA CCCTTTCCAG GCTCCGGGCT CAGTGTTCCG 
CAGCACTCGG AACTGACCAA CCCAGCCAGC AATCTGTCTA CTGTAGCTAT CCTCCCTGTG 
TGTGCTGAGG TGCCCATGAT GGCCTTTACT CTGGAACTGC AGCATGCTCT GCAAGCTATT 
GGTCCCACGC TCCTCCTTAA CAGTGACGTC ATCCGGGCAC TCCTGGGGGC TTCAGCACTG 
GATAGCATTC AAGAATTCCG GCTGTCAGGG TGGTTGGCCC AGCAGGAAGA TGCCCATCGC 
ATTGTGCTCT ACCAAACTGA CACATCCCTG ACGCCTTGGA CCGTCCGTTG TCTGCGCCAG 
GCCGACTGCA TCCTCATTGT GGGCCTGGGG GACCAGGAAC CCACTGTTGG CCAGCTGGAG 
CAAATGTTAG AGAACACTGC TGTCCGTGCC TTGAAGCAAC TGGTTTTGCT GCACCGGGAG 
GAAGGCCCTG GTCCCACGCG CACTGTAGAG TGGCTCAACA TGCGCAGCTG GTGCTCAGGG 
CACCTGCACC TGCGCTGTCC TCGCCGCCTC TTCTCACGGC GCAGCCCAGC GAAACTTCAT 
GAGCTGTATG AGAAGGTTTT CTCCAGGCGT GCAGACCGTC ACAGCGACTT CTCCCGCTTG 
GCACGAGTAC TCACAGGAAA CACTATTGCT CTGGTGCTGG GTGGGGGCGG AGCCAGAGGC 
TGCTCGCATA TTGGGGTGCT GAAGGCATTA GAGGAGGCAG GAGTCCCAGT CGACCTTGTG 
GGAGGCACAT CCATAGGTTC CTTCATTGGG GCCTTGTATG CCGAGGAACG CAGCGCCAGC 
CGAACTAAAC AACGAGCCCG GGAGTGGGCC AAGAGCATGA CTTCTGTACT GGAGCCTGTA 
TTGGACCTCA CATATCCTGT CACCTCCATG TTTACTGGCT CAGCCTTTAA CAGAAGTATC 
CACCGTGTCT TCCAGGATAA GCAGATTGAG GACCTGTGGC TGCCTTACTT CAATGTGACC 
ACAGACATCA CTGCCTCTGC CATGCGCGTC CACAAAGATG GCTCCCTGTG GCGGTATGTA 
CGTGCCAGCA TGACGCTCTC GGGCTACCTA CCCCCGCTGT GCGACCCAAA GGATGGGCAC 
CTGCTCATGG ATGGTGGCTA CATCAACAAC CTGCCAGCGG ATATTGCCCG AAGCATGGGA 
GCCAAAACGG TCATTGCCAT CGACGTTGGA AGCCAGGATG AGACAGATCT CAGCACCTAT 
GGGGACAGCC TGTCTGGCTG GTGGTTGCTG TGGAAAAGGC TAAACCCCTG GGCAGACAAG 
GTAAAGGTTC CAGACATGGC TGAGATCCAG TCCCGCTTGG CGTACGTGTC CTGTGTGCGG 
CAGCTAGAAG TCGTCAAGTC CAGCTCCTAT TGCGAGTACC TTCGTCCATC CATTGACTGC 
TTCAAGACCA TGGACTTTGG AAAGTTTGAC CAGATCTATG ATGTGGGTTA CCAGTATGGG 
AAGGCTGTCT TTGGAGGCTG GACCCGTGGT GAAGTCATTG AGAAAATGCT CACAGACCGG 
AGATCTACAG ACCTTAATGA GAGTCGCCGT GCAGATATAC TTGCCTTCCC AAGTTCTGGG 
TTCACTGACT TGGCTGAGAT TGTGTCCCGG ATCGAGCCAC CAACAAGCTA CGTCTCTGAT 
GGCTGTGCTG ATGGGGAGGA GTCGGATTGC TTGACAGAGT ATGAGGAAGA TGCAGGACCA 
GACTGCTCAA GAGATGAAGG GGGCTCCCCT GAGGGTGCCA GCCCTAGCAC TGCCTCAGAG 
GTGGAAGAAG AGAAGTCCAC ACTCCGGCAA CGACGCTTTC TGCCTCAGGA GACTCCCAGC 
TCAGTCGCAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508831.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508894.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X7, mRNA.

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CloneID	OMu05750
Clone ID Related Accession (Same CDS sequence)	NM_001122818.2 , XM_006508831.3 , XM_006508831.2 , XM_006508831.4
Accession Version	XM_006508831.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508831.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGGAGCTC 
CGGGAGCAGC CAGCGGGTGC CTGTGAATAT AGCTACTGTG AGGATGAGTC AGCCACAGGC 
GGATGTCCCT TTGGGCCCTA CCAGGGCCGC CAGACAAGCA GCATCTTTGA GGCTGCAAAG 
AGAGAGCTAG CCAAGCTGAT GCGGATTGAG GACCCCTCTC TACTGAACAG CAGAGTCTTG 
CTACATCATG CCAAAGCCGG CACCATCATA GCCCGCCAAG GGGACCAGGA TGTGAGCCTG 
CACTTTGTGC TCTGGGGCTG CCTGCACGTG TACCAGCGCA TGATTGACAA GGCTGAGGAA 
GTGTGCCTAT TTGTGGCACA GCCAGGGGAG CTGGTGGGGC AACTGGCGGT GCTCACTGGG 
GAACCTCTCA TCTTCACACT ACGTGCCCAG AGAGACTGCA CCTTCCTGCG GATCTCCAAA 
TCCCACTTCT ATGAGATCAT GCGTGCACAA CCCAGTGTGG TACTGAGTGC AGCTCACACG 
GTGGCTGCTA GAATGTCCCC CTTCGTGCGC CAGATGGACT TTGCCATTGA CTGGACAGCT 
GTGGAGGCTG GTCGTGCGCT CTACAGGCAG GGAGACCGCT CCGACTGCAC CTACATTGTA 
CTCAATGGAC GTCTCCGTAG TGTCATCCAA CGAGGCAGTG GCAAGAAGGA GCTAGTTGGG 
GAGTATGGTC GAGGGGATCT CATTGGCGTG GTGGAGGCAC TGACCCGGCA GCCACGAGCC 
ACCACTGTAC ACGCAGTGCG AGACACTGAG CTGGCCAAGC TTCCCGAGGG CACCTTAGGC 
CACATCAAAC GTCGGTACCC ACAGGTTGTG ACCCGCCTTA TTCATCTGCT AAGCCAGAAA 
ATTCTAGGCA ATCTGCAGCA GTTGCAAGGA CCCTTTCCAG GCTCCGGGCT CAGTGTTCCG 
CAGCACTCGG AACTGACCAA CCCAGCCAGC AATCTGTCTA CTGTAGCTAT CCTCCCTGTG 
TGTGCTGAGG TGCCCATGAT GGCCTTTACT CTGGAACTGC AGCATGCTCT GCAAGCTATT 
GGTCCCACGC TCCTCCTTAA CAGTGACGTC ATCCGGGCAC TCCTGGGGGC TTCAGCACTG 
GATAGCATTC AAGAATTCCG GCTGTCAGGG TGGTTGGCCC AGCAGGAAGA TGCCCATCGC 
ATTGTGCTCT ACCAAACTGA CACATCCCTG ACGCCTTGGA CCGTCCGTTG TCTGCGCCAG 
GCCGACTGCA TCCTCATTGT GGGCCTGGGG GACCAGGAAC CCACTGTTGG CCAGCTGGAG 
CAAATGTTAG AGAACACTGC TGTCCGTGCC TTGAAGCAAC TGGTTTTGCT GCACCGGGAG 
GAAGGCCCTG GTCCCACGCG CACTGTAGAG TGGCTCAACA TGCGCAGCTG GTGCTCAGGG 
CACCTGCACC TGCGCTGTCC TCGCCGCCTC TTCTCACGGC GCAGCCCAGC GAAACTTCAT 
GAGCTGTATG AGAAGGTTTT CTCCAGGCGT GCAGACCGTC ACAGCGACTT CTCCCGCTTG 
GCACGAGTAC TCACAGGAAA CACTATTGCT CTGGTGCTGG GTGGGGGCGG AGCCAGAGGC 
TGCTCGCATA TTGGGGTGCT GAAGGCATTA GAGGAGGCAG GAGTCCCAGT CGACCTTGTG 
GGAGGCACAT CCATAGGTTC CTTCATTGGG GCCTTGTATG CCGAGGAACG CAGCGCCAGC 
CGAACTAAAC AACGAGCCCG GGAGTGGGCC AAGAGCATGA CTTCTGTACT GGAGCCTGTA 
TTGGACCTCA CATATCCTGT CACCTCCATG TTTACTGGCT CAGCCTTTAA CAGAAGTATC 
CACCGTGTCT TCCAGGATAA GCAGATTGAG GACCTGTGGC TGCCTTACTT CAATGTGACC 
ACAGACATCA CTGCCTCTGC CATGCGCGTC CACAAAGATG GCTCCCTGTG GCGGTATGTA 
CGTGCCAGCA TGACGCTCTC GGGCTACCTA CCCCCGCTGT GCGACCCAAA GGATGGGCAC 
CTGCTCATGG ATGGTGGCTA CATCAACAAC CTGCCAGCGG ATATTGCCCG AAGCATGGGA 
GCCAAAACGG TCATTGCCAT CGACGTTGGA AGCCAGGATG AGACAGATCT CAGCACCTAT 
GGGGACAGCC TGTCTGGCTG GTGGTTGCTG TGGAAAAGGC TAAACCCCTG GGCAGACAAG 
GTAAAGGTTC CAGACATGGC TGAGATCCAG TCCCGCTTGG CGTACGTGTC CTGTGTGCGG 
CAGCTAGAAG TCGTCAAGTC CAGCTCCTAT TGCGAGTACC TTCGTCCATC CATTGACTGC 
TTCAAGACCA TGGACTTTGG AAAGTTTGAC CAGATCTATG ATGTGGGTTA CCAGTATGGG 
AAGGCTGTCT TTGGAGGCTG GACCCGTGGT GAAGTCATTG AGAAAATGCT CACAGACCGG 
AGATCTACAG ACCTTAATGA GAGTCGCCGT GCAGATATAC TTGCCTTCCC AAGTTCTGGG 
TTCACTGACT TGGCTGAGAT TGTGTCCCGG ATCGAGCCAC CAACAAGCTA CGTCTCTGAT 
GGCTGTGCTG ATGGGGAGGA GTCGGATTGC TTGACAGAGT ATGAGGAAGA TGCAGGACCA 
GACTGCTCAA GAGATGAAGG GGGCTCCCCT GAGGGTGCCA GCCCTAGCAC TGCCTCAGAG 
GTGGAAGAAG AGAAGTCCAC ACTCCGGCAA CGACGCTTTC TGCCTCAGGA GACTCCCAGC 
TCAGTCGCAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508894.1
CDS	361..4398
Translation	MEESLQPKDCRLQDLLPSPKQSMEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508831.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508831.4

ORF Insert Sequence:

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGGAGCTC 
CGGGAGCAGC CAGCGGGTGC CTGTGAATAT AGCTACTGTG AGGATGAGTC AGCCACAGGC 
GGATGTCCCT TTGGGCCCTA CCAGGGCCGC CAGACAAGCA GCATCTTTGA GGCTGCAAAG 
AGAGAGCTAG CCAAGCTGAT GCGGATTGAG GACCCCTCTC TACTGAACAG CAGAGTCTTG 
CTACATCATG CCAAAGCCGG CACCATCATA GCCCGCCAAG GGGACCAGGA TGTGAGCCTG 
CACTTTGTGC TCTGGGGCTG CCTGCACGTG TACCAGCGCA TGATTGACAA GGCTGAGGAA 
GTGTGCCTAT TTGTGGCACA GCCAGGGGAG CTGGTGGGGC AACTGGCGGT GCTCACTGGG 
GAACCTCTCA TCTTCACACT ACGTGCCCAG AGAGACTGCA CCTTCCTGCG GATCTCCAAA 
TCCCACTTCT ATGAGATCAT GCGTGCACAA CCCAGTGTGG TACTGAGTGC AGCTCACACG 
GTGGCTGCTA GAATGTCCCC CTTCGTGCGC CAGATGGACT TTGCCATTGA CTGGACAGCT 
GTGGAGGCTG GTCGTGCGCT CTACAGGCAG GGAGACCGCT CCGACTGCAC CTACATTGTA 
CTCAATGGAC GTCTCCGTAG TGTCATCCAA CGAGGCAGTG GCAAGAAGGA GCTAGTTGGG 
GAGTATGGTC GAGGGGATCT CATTGGCGTG GTGGAGGCAC TGACCCGGCA GCCACGAGCC 
ACCACTGTAC ACGCAGTGCG AGACACTGAG CTGGCCAAGC TTCCCGAGGG CACCTTAGGC 
CACATCAAAC GTCGGTACCC ACAGGTTGTG ACCCGCCTTA TTCATCTGCT AAGCCAGAAA 
ATTCTAGGCA ATCTGCAGCA GTTGCAAGGA CCCTTTCCAG GCTCCGGGCT CAGTGTTCCG 
CAGCACTCGG AACTGACCAA CCCAGCCAGC AATCTGTCTA CTGTAGCTAT CCTCCCTGTG 
TGTGCTGAGG TGCCCATGAT GGCCTTTACT CTGGAACTGC AGCATGCTCT GCAAGCTATT 
GGTCCCACGC TCCTCCTTAA CAGTGACGTC ATCCGGGCAC TCCTGGGGGC TTCAGCACTG 
GATAGCATTC AAGAATTCCG GCTGTCAGGG TGGTTGGCCC AGCAGGAAGA TGCCCATCGC 
ATTGTGCTCT ACCAAACTGA CACATCCCTG ACGCCTTGGA CCGTCCGTTG TCTGCGCCAG 
GCCGACTGCA TCCTCATTGT GGGCCTGGGG GACCAGGAAC CCACTGTTGG CCAGCTGGAG 
CAAATGTTAG AGAACACTGC TGTCCGTGCC TTGAAGCAAC TGGTTTTGCT GCACCGGGAG 
GAAGGCCCTG GTCCCACGCG CACTGTAGAG TGGCTCAACA TGCGCAGCTG GTGCTCAGGG 
CACCTGCACC TGCGCTGTCC TCGCCGCCTC TTCTCACGGC GCAGCCCAGC GAAACTTCAT 
GAGCTGTATG AGAAGGTTTT CTCCAGGCGT GCAGACCGTC ACAGCGACTT CTCCCGCTTG 
GCACGAGTAC TCACAGGAAA CACTATTGCT CTGGTGCTGG GTGGGGGCGG AGCCAGAGGC 
TGCTCGCATA TTGGGGTGCT GAAGGCATTA GAGGAGGCAG GAGTCCCAGT CGACCTTGTG 
GGAGGCACAT CCATAGGTTC CTTCATTGGG GCCTTGTATG CCGAGGAACG CAGCGCCAGC 
CGAACTAAAC AACGAGCCCG GGAGTGGGCC AAGAGCATGA CTTCTGTACT GGAGCCTGTA 
TTGGACCTCA CATATCCTGT CACCTCCATG TTTACTGGCT CAGCCTTTAA CAGAAGTATC 
CACCGTGTCT TCCAGGATAA GCAGATTGAG GACCTGTGGC TGCCTTACTT CAATGTGACC 
ACAGACATCA CTGCCTCTGC CATGCGCGTC CACAAAGATG GCTCCCTGTG GCGGTATGTA 
CGTGCCAGCA TGACGCTCTC GGGCTACCTA CCCCCGCTGT GCGACCCAAA GGATGGGCAC 
CTGCTCATGG ATGGTGGCTA CATCAACAAC CTGCCAGCGG ATATTGCCCG AAGCATGGGA 
GCCAAAACGG TCATTGCCAT CGACGTTGGA AGCCAGGATG AGACAGATCT CAGCACCTAT 
GGGGACAGCC TGTCTGGCTG GTGGTTGCTG TGGAAAAGGC TAAACCCCTG GGCAGACAAG 
GTAAAGGTTC CAGACATGGC TGAGATCCAG TCCCGCTTGG CGTACGTGTC CTGTGTGCGG 
CAGCTAGAAG TCGTCAAGTC CAGCTCCTAT TGCGAGTACC TTCGTCCATC CATTGACTGC 
TTCAAGACCA TGGACTTTGG AAAGTTTGAC CAGATCTATG ATGTGGGTTA CCAGTATGGG 
AAGGCTGTCT TTGGAGGCTG GACCCGTGGT GAAGTCATTG AGAAAATGCT CACAGACCGG 
AGATCTACAG ACCTTAATGA GAGTCGCCGT GCAGATATAC TTGCCTTCCC AAGTTCTGGG 
TTCACTGACT TGGCTGAGAT TGTGTCCCGG ATCGAGCCAC CAACAAGCTA CGTCTCTGAT 
GGCTGTGCTG ATGGGGAGGA GTCGGATTGC TTGACAGAGT ATGAGGAAGA TGCAGGACCA 
GACTGCTCAA GAGATGAAGG GGGCTCCCCT GAGGGTGCCA GCCCTAGCAC TGCCTCAGAG 
GTGGAAGAAG AGAAGTCCAC ACTCCGGCAA CGACGCTTTC TGCCTCAGGA GACTCCCAGC 
TCAGTCGCAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508824.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508887.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X1, mRNA.

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CloneID	OMu40424
Clone ID Related Accession (Same CDS sequence)	XM_006508824.4 , XM_006508824.3
Accession Version	XM_006508824.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508824.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGACAC CGAGTCACGA ACTGAATACA ACCTCCTCCG GGGCAGAGGT GATTCAAAAG 
ACCCTGGAGG AGGGCTTGGG GAGACGGATA TGCGTTGCCC AGCCTGTGCC GTTCGTTCCG 
CAGGTGCTGG GCGTGATGAT CGGGGCCGGA GTGGCGGTGC TGGTCACCGC CGTGCTCATC 
CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG AAAACTCCAG CCCCGGAGGG TCCCCGGTAT 
CGGTTCCGGA AGCGGGACAA AGTGCTTTTC TATGGCCGGA AGATTATGCG GAAGGTGTCA 
CAGTCCACTT CTTCCCTGGT GGACACATCA GTCTCCACCA CTTCCCGGCC CCGCATGAAG 
AAGAAACTGA AGATGCTTAA CATTGCCAAG AAGATCTTAC GTATCCAAAA GGAGACTCCG 
ACCCTACAAC GGAAGGAGCC CCCACCTTCA GTGCTGGAGG CTGACCTCAC AGAGGGAGAC 
CTGGCCAATT CCCACCTACC CTCTGAGGTA CTCTACATGC TTAAGAATGT TCGGGTGCTA 
GGTCACTTTG AGAAGCCGCT CTTCCTGGAG CTCTGCCGGC ACATGGTCTT CCAGCGTCTC 
GGGCAGGGGG ACTATGTTTT TCGGCCCGGT CAACCAGATG CCAGCATCTA TGTGGTTCAA 
GATGGGCTGC TGGAGCTCTG CCTACCAGGG CCTGATGGGA AGGAGTGTGT GGTGAAGGAG 
GTGGTTCCTG GAGACAGCGT CAACAGCCTT CTGAGCATCC TTGATGTCAT CACGGGTCAC 
CAGCATCCCC AGCGGACTGT GTCAGCCAGG GCTGCACGGG ACTCCACAGT GCTGAGACTC 
CCAGTAGAAG CCTTCTCTGC TGTCTTCACC AAGTACCCTG AGAGTTTGGT GCGAGTGGTA 
CAGATCATCA TGGTAAGGCT GCAGAGAGTC ACCTTCCTGG CGCTTCACAA TTACCTGGGT 
CTGACCAATG AACTCTTCAG TCATGAGATC CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC 
CTCCCGACCC GAACCAGCCC TGTCCGTGGC TCTAAACGGG TAGTCAGCAC CTCAGGTACT 
GAGGACACCT CAAAGGAGAC CTCTGGCCGG CCCCTGGACT CCATTGGAGC TCCTCTGCCT 
GGACCTGCAG GGGATCCCGT GAAGCCCACA TCCTTAGAAG CACCCCCAGC CCCGTTACTG 
AGCCGCTGCA TCTCCATGCC AGTAGACATC TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT 
TTTGACATGG CCTATGAACG TGGACGGATA TCTGTTTCTC TTCAAGAAGA GGCATCTGGG 
GGACCTCAGA CAGCATCCCC TAGGGAGCTC CGGGAGCAGC CAGCGGGTGC CTGTGAATAT 
AGCTACTGTG AGGATGAGTC AGCCACAGGC GGATGTCCCT TTGGGCCCTA CCAGGGCCGC 
CAGACAAGCA GCATCTTTGA GGCTGCAAAG AGAGAGCTAG CCAAGCTGAT GCGGATTGAG 
GACCCCTCTC TACTGAACAG CAGAGTCTTG CTACATCATG CCAAAGCCGG CACCATCATA 
GCCCGCCAAG GGGACCAGGA TGTGAGCCTG CACTTTGTGC TCTGGGGCTG CCTGCACGTG 
TACCAGCGCA TGATTGACAA GGCTGAGGAA GTGTGCCTAT TTGTGGCACA GCCAGGGGAG 
CTGGTGGGGC AACTGGCGGT GCTCACTGGG GAACCTCTCA TCTTCACACT ACGTGCCCAG 
AGAGACTGCA CCTTCCTGCG GATCTCCAAA TCCCACTTCT ATGAGATCAT GCGTGCACAA 
CCCAGTGTGG TACTGAGTGC AGCTCACACG GTGGCTGCTA GAATGTCCCC CTTCGTGCGC 
CAGATGGACT TTGCCATTGA CTGGACAGCT GTGGAGGCTG GTCGTGCGCT CTACAGGCAG 
GGAGACCGCT CCGACTGCAC CTACATTGTA CTCAATGGAC GTCTCCGTAG TGTCATCCAA 
CGAGGCAGTG GCAAGAAGGA GCTAGTTGGG GAGTATGGTC GAGGGGATCT CATTGGCGTG 
GTGGAGGCAC TGACCCGGCA GCCACGAGCC ACCACTGTAC ACGCAGTGCG AGACACTGAG 
CTGGCCAAGC TTCCCGAGGG CACCTTAGGC CACATCAAAC GTCGGTACCC ACAGGTTGTG 
ACCCGCCTTA TTCATCTGCT AAGCCAGAAA ATTCTAGGCA ATCTGCAGCA GTTGCAAGGA 
CCCTTTCCAG GCTCCGGGCT CAGTGTTCCG CAGCACTCGG AACTGACCAA CCCAGCCAGC 
AATCTGTCTA CTGTAGCTAT CCTCCCTGTG TGTGCTGAGG TGCCCATGAT GGCCTTTACT 
CTGGAACTGC AGCATGCTCT GCAAGCTATT GGTCCCACGC TCCTCCTTAA CAGTGACGTC 
ATCCGGGCAC TCCTGGGGGC TTCAGCACTG GATAGCATTC AAGAATTCCG GCTGTCAGGG 
TGGTTGGCCC AGCAGGAAGA TGCCCATCGC ATTGTGCTCT ACCAAACTGA CACATCCCTG 
ACGCCTTGGA CCGTCCGTTG TCTGCGCCAG GCCGACTGCA TCCTCATTGT GGGCCTGGGG 
GACCAGGAAC CCACTGTTGG CCAGCTGGAG CAAATGTTAG AGAACACTGC TGTCCGTGCC 
TTGAAGCAAC TGGTTTTGCT GCACCGGGAG GAAGGCCCTG GTCCCACGCG CACTGTAGAG 
TGGCTCAACA TGCGCAGCTG GTGCTCAGGG CACCTGCACC TGCGCTGTCC TCGCCGCCTC 
TTCTCACGGC GCAGCCCAGC GAAACTTCAT GAGCTGTATG AGAAGGTTTT CTCCAGGCGT 
GCAGACCGTC ACAGCGACTT CTCCCGCTTG GCACGAGTAC TCACAGGAAA CACTATTGCT 
CTGGTGCTGG GTGGGGGCGG AGCCAGAGGC TGCTCGCATA TTGGGGTGCT GAAGGCATTA 
GAGGAGGCAG GAGTCCCAGT CGACCTTGTG GGAGGCACAT CCATAGGTTC CTTCATTGGG 
GCCTTGTATG CCGAGGAACG CAGCGCCAGC CGAACTAAAC AACGAGCCCG GGAGTGGGCC 
AAGAGCATGA CTTCTGTACT GGAGCCTGTA TTGGACCTCA CATATCCTGT CACCTCCATG 
TTTACTGGCT CAGCCTTTAA CAGAAGTATC CACCGTGTCT TCCAGGATAA GCAGATTGAG 
GACCTGTGGC TGCCTTACTT CAATGTGACC ACAGACATCA CTGCCTCTGC CATGCGCGTC 
CACAAAGATG GCTCCCTGTG GCGGTATGTA CGTGCCAGCA TGACGCTCTC GGGCTACCTA 
CCCCCGCTGT GCGACCCAAA GGATGGGCAC CTGCTCATGG ATGGTGGCTA CATCAACAAC 
CTGCCAGCGG ATATTGCCCG AAGCATGGGA GCCAAAACGG TCATTGCCAT CGACGTTGGA 
AGCCAGGATG AGACAGATCT CAGCACCTAT GGGGACAGCC TGTCTGGCTG GTGGTTGCTG 
TGGAAAAGGC TAAACCCCTG GGCAGACAAG GTAAAGGTTC CAGACATGGC TGAGATCCAG 
TCCCGCTTGG CGTACGTGTC CTGTGTGCGG CAGCTAGAAG TCGTCAAGTC CAGCTCCTAT 
TGCGAGTACC TTCGTCCATC CATTGACTGC TTCAAGACCA TGGACTTTGG AAAGTTTGAC 
CAGATCTATG ATGTGGGTTA CCAGTATGGG AAGGCTGTCT TTGGAGGCTG GACCCGTGGT 
GAAGTCATTG AGAAAATGCT CACAGACCGG AGATCTACAG ACCTTAATGA GAGTCGCCGT 
GCAGATATAC TTGCCTTCCC AAGTTCTGGG TTCACTGACT TGGCTGAGAT TGTGTCCCGG 
ATCGAGCCAC CAACAAGCTA CGTCTCTGAT GGCTGTGCTG ATGGGGAGGA GTCGGATTGC 
TTGACAGAGT ATGAGGAAGA TGCAGGACCA GACTGCTCAA GAGATGAAGG GGGCTCCCCT 
GAGGGTGCCA GCCCTAGCAC TGCCTCAGAG GTGGAAGAAG AGAAGTCCAC ACTCCGGCAA 
CGACGCTTTC TGCCTCAGGA GACTCCCAGC TCAGTCGCAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508887.1
CDS	143..4210
Translation	MGTPSHELNTTSSGAEVIQKTLEEGLGRRICVAQPVPFVPQVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508824.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508824.4

ORF Insert Sequence:

ATGGGGACAC CGAGTCACGA ACTGAATACA ACCTCCTCCG GGGCAGAGGT GATTCAAAAG 
ACCCTGGAGG AGGGCTTGGG GAGACGGATA TGCGTTGCCC AGCCTGTGCC GTTCGTTCCG 
CAGGTGCTGG GCGTGATGAT CGGGGCCGGA GTGGCGGTGC TGGTCACCGC CGTGCTCATC 
CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG AAAACTCCAG CCCCGGAGGG TCCCCGGTAT 
CGGTTCCGGA AGCGGGACAA AGTGCTTTTC TATGGCCGGA AGATTATGCG GAAGGTGTCA 
CAGTCCACTT CTTCCCTGGT GGACACATCA GTCTCCACCA CTTCCCGGCC CCGCATGAAG 
AAGAAACTGA AGATGCTTAA CATTGCCAAG AAGATCTTAC GTATCCAAAA GGAGACTCCG 
ACCCTACAAC GGAAGGAGCC CCCACCTTCA GTGCTGGAGG CTGACCTCAC AGAGGGAGAC 
CTGGCCAATT CCCACCTACC CTCTGAGGTA CTCTACATGC TTAAGAATGT TCGGGTGCTA 
GGTCACTTTG AGAAGCCGCT CTTCCTGGAG CTCTGCCGGC ACATGGTCTT CCAGCGTCTC 
GGGCAGGGGG ACTATGTTTT TCGGCCCGGT CAACCAGATG CCAGCATCTA TGTGGTTCAA 
GATGGGCTGC TGGAGCTCTG CCTACCAGGG CCTGATGGGA AGGAGTGTGT GGTGAAGGAG 
GTGGTTCCTG GAGACAGCGT CAACAGCCTT CTGAGCATCC TTGATGTCAT CACGGGTCAC 
CAGCATCCCC AGCGGACTGT GTCAGCCAGG GCTGCACGGG ACTCCACAGT GCTGAGACTC 
CCAGTAGAAG CCTTCTCTGC TGTCTTCACC AAGTACCCTG AGAGTTTGGT GCGAGTGGTA 
CAGATCATCA TGGTAAGGCT GCAGAGAGTC ACCTTCCTGG CGCTTCACAA TTACCTGGGT 
CTGACCAATG AACTCTTCAG TCATGAGATC CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC 
CTCCCGACCC GAACCAGCCC TGTCCGTGGC TCTAAACGGG TAGTCAGCAC CTCAGGTACT 
GAGGACACCT CAAAGGAGAC CTCTGGCCGG CCCCTGGACT CCATTGGAGC TCCTCTGCCT 
GGACCTGCAG GGGATCCCGT GAAGCCCACA TCCTTAGAAG CACCCCCAGC CCCGTTACTG 
AGCCGCTGCA TCTCCATGCC AGTAGACATC TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT 
TTTGACATGG CCTATGAACG TGGACGGATA TCTGTTTCTC TTCAAGAAGA GGCATCTGGG 
GGACCTCAGA CAGCATCCCC TAGGGAGCTC CGGGAGCAGC CAGCGGGTGC CTGTGAATAT 
AGCTACTGTG AGGATGAGTC AGCCACAGGC GGATGTCCCT TTGGGCCCTA CCAGGGCCGC 
CAGACAAGCA GCATCTTTGA GGCTGCAAAG AGAGAGCTAG CCAAGCTGAT GCGGATTGAG 
GACCCCTCTC TACTGAACAG CAGAGTCTTG CTACATCATG CCAAAGCCGG CACCATCATA 
GCCCGCCAAG GGGACCAGGA TGTGAGCCTG CACTTTGTGC TCTGGGGCTG CCTGCACGTG 
TACCAGCGCA TGATTGACAA GGCTGAGGAA GTGTGCCTAT TTGTGGCACA GCCAGGGGAG 
CTGGTGGGGC AACTGGCGGT GCTCACTGGG GAACCTCTCA TCTTCACACT ACGTGCCCAG 
AGAGACTGCA CCTTCCTGCG GATCTCCAAA TCCCACTTCT ATGAGATCAT GCGTGCACAA 
CCCAGTGTGG TACTGAGTGC AGCTCACACG GTGGCTGCTA GAATGTCCCC CTTCGTGCGC 
CAGATGGACT TTGCCATTGA CTGGACAGCT GTGGAGGCTG GTCGTGCGCT CTACAGGCAG 
GGAGACCGCT CCGACTGCAC CTACATTGTA CTCAATGGAC GTCTCCGTAG TGTCATCCAA 
CGAGGCAGTG GCAAGAAGGA GCTAGTTGGG GAGTATGGTC GAGGGGATCT CATTGGCGTG 
GTGGAGGCAC TGACCCGGCA GCCACGAGCC ACCACTGTAC ACGCAGTGCG AGACACTGAG 
CTGGCCAAGC TTCCCGAGGG CACCTTAGGC CACATCAAAC GTCGGTACCC ACAGGTTGTG 
ACCCGCCTTA TTCATCTGCT AAGCCAGAAA ATTCTAGGCA ATCTGCAGCA GTTGCAAGGA 
CCCTTTCCAG GCTCCGGGCT CAGTGTTCCG CAGCACTCGG AACTGACCAA CCCAGCCAGC 
AATCTGTCTA CTGTAGCTAT CCTCCCTGTG TGTGCTGAGG TGCCCATGAT GGCCTTTACT 
CTGGAACTGC AGCATGCTCT GCAAGCTATT GGTCCCACGC TCCTCCTTAA CAGTGACGTC 
ATCCGGGCAC TCCTGGGGGC TTCAGCACTG GATAGCATTC AAGAATTCCG GCTGTCAGGG 
TGGTTGGCCC AGCAGGAAGA TGCCCATCGC ATTGTGCTCT ACCAAACTGA CACATCCCTG 
ACGCCTTGGA CCGTCCGTTG TCTGCGCCAG GCCGACTGCA TCCTCATTGT GGGCCTGGGG 
GACCAGGAAC CCACTGTTGG CCAGCTGGAG CAAATGTTAG AGAACACTGC TGTCCGTGCC 
TTGAAGCAAC TGGTTTTGCT GCACCGGGAG GAAGGCCCTG GTCCCACGCG CACTGTAGAG 
TGGCTCAACA TGCGCAGCTG GTGCTCAGGG CACCTGCACC TGCGCTGTCC TCGCCGCCTC 
TTCTCACGGC GCAGCCCAGC GAAACTTCAT GAGCTGTATG AGAAGGTTTT CTCCAGGCGT 
GCAGACCGTC ACAGCGACTT CTCCCGCTTG GCACGAGTAC TCACAGGAAA CACTATTGCT 
CTGGTGCTGG GTGGGGGCGG AGCCAGAGGC TGCTCGCATA TTGGGGTGCT GAAGGCATTA 
GAGGAGGCAG GAGTCCCAGT CGACCTTGTG GGAGGCACAT CCATAGGTTC CTTCATTGGG 
GCCTTGTATG CCGAGGAACG CAGCGCCAGC CGAACTAAAC AACGAGCCCG GGAGTGGGCC 
AAGAGCATGA CTTCTGTACT GGAGCCTGTA TTGGACCTCA CATATCCTGT CACCTCCATG 
TTTACTGGCT CAGCCTTTAA CAGAAGTATC CACCGTGTCT TCCAGGATAA GCAGATTGAG 
GACCTGTGGC TGCCTTACTT CAATGTGACC ACAGACATCA CTGCCTCTGC CATGCGCGTC 
CACAAAGATG GCTCCCTGTG GCGGTATGTA CGTGCCAGCA TGACGCTCTC GGGCTACCTA 
CCCCCGCTGT GCGACCCAAA GGATGGGCAC CTGCTCATGG ATGGTGGCTA CATCAACAAC 
CTGCCAGCGG ATATTGCCCG AAGCATGGGA GCCAAAACGG TCATTGCCAT CGACGTTGGA 
AGCCAGGATG AGACAGATCT CAGCACCTAT GGGGACAGCC TGTCTGGCTG GTGGTTGCTG 
TGGAAAAGGC TAAACCCCTG GGCAGACAAG GTAAAGGTTC CAGACATGGC TGAGATCCAG 
TCCCGCTTGG CGTACGTGTC CTGTGTGCGG CAGCTAGAAG TCGTCAAGTC CAGCTCCTAT 
TGCGAGTACC TTCGTCCATC CATTGACTGC TTCAAGACCA TGGACTTTGG AAAGTTTGAC 
CAGATCTATG ATGTGGGTTA CCAGTATGGG AAGGCTGTCT TTGGAGGCTG GACCCGTGGT 
GAAGTCATTG AGAAAATGCT CACAGACCGG AGATCTACAG ACCTTAATGA GAGTCGCCGT 
GCAGATATAC TTGCCTTCCC AAGTTCTGGG TTCACTGACT TGGCTGAGAT TGTGTCCCGG 
ATCGAGCCAC CAACAAGCTA CGTCTCTGAT GGCTGTGCTG ATGGGGAGGA GTCGGATTGC 
TTGACAGAGT ATGAGGAAGA TGCAGGACCA GACTGCTCAA GAGATGAAGG GGGCTCCCCT 
GAGGGTGCCA GCCCTAGCAC TGCCTCAGAG GTGGAAGAAG AGAAGTCCAC ACTCCGGCAA 
CGACGCTTTC TGCCTCAGGA GACTCCCAGC TCAGTCGCAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508825.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508888.3 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X2, mRNA.

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CloneID	OMu40425
Clone ID Related Accession (Same CDS sequence)	XM_006508825.4
Accession Version	XM_006508825.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508825.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG TCAATCTAAC AATTTTCCAG GACTGCAGGC TACAAGACCT GCTACCTAGT 
CCGAAACAAT CGATGGAGGC CCCACTGCAA ACCGGAATGG TGCTGGGCGT GATGATCGGG 
GCCGGAGTGG CGGTGCTGGT CACCGCCGTG CTCATCCTCT TGGTGGTGCG AAGGCTGCGG 
GTGCAGAAAA CTCCAGCCCC GGAGGGTCCC CGGTATCGGT TCCGGAAGCG GGACAAAGTG 
CTTTTCTATG GCCGGAAGAT TATGCGGAAG GTGTCACAGT CCACTTCTTC CCTGGTGGAC 
ACATCAGTCT CCACCACTTC CCGGCCCCGC ATGAAGAAGA AACTGAAGAT GCTTAACATT 
GCCAAGAAGA TCTTACGTAT CCAAAAGGAG ACTCCGACCC TACAACGGAA GGAGCCCCCA 
CCTTCAGTGC TGGAGGCTGA CCTCACAGAG GGAGACCTGG CCAATTCCCA CCTACCCTCT 
GAGGTACTCT ACATGCTTAA GAATGTTCGG GTGCTAGGTC ACTTTGAGAA GCCGCTCTTC 
CTGGAGCTCT GCCGGCACAT GGTCTTCCAG CGTCTCGGGC AGGGGGACTA TGTTTTTCGG 
CCCGGTCAAC CAGATGCCAG CATCTATGTG GTTCAAGATG GGCTGCTGGA GCTCTGCCTA 
CCAGGGCCTG ATGGGAAGGA GTGTGTGGTG AAGGAGGTGG TTCCTGGAGA CAGCGTCAAC 
AGCCTTCTGA GCATCCTTGA TGTCATCACG GGTCACCAGC ATCCCCAGCG GACTGTGTCA 
GCCAGGGCTG CACGGGACTC CACAGTGCTG AGACTCCCAG TAGAAGCCTT CTCTGCTGTC 
TTCACCAAGT ACCCTGAGAG TTTGGTGCGA GTGGTACAGA TCATCATGGT AAGGCTGCAG 
AGAGTCACCT TCCTGGCGCT TCACAATTAC CTGGGTCTGA CCAATGAACT CTTCAGTCAT 
GAGATCCAGC CCCTACGCCT TTTCCCCAGC CCCGGCCTCC CGACCCGAAC CAGCCCTGTC 
CGTGGCTCTA AACGGGTAGT CAGCACCTCA GGTACTGAGG ACACCTCAAA GGAGACCTCT 
GGCCGGCCCC TGGACTCCAT TGGAGCTCCT CTGCCTGGAC CTGCAGGGGA TCCCGTGAAG 
CCCACATCCT TAGAAGCACC CCCAGCCCCG TTACTGAGCC GCTGCATCTC CATGCCAGTA 
GACATCTCAG GCTTGCAAGG TGGCCCTCGT TCTGATTTTG ACATGGCCTA TGAACGTGGA 
CGGATATCTG TTTCTCTTCA AGAAGAGGCA TCTGGGGGAC CTCAGACAGC ATCCCCTAGG 
ACCCCCACTC AGGAGCTCCG GGAGCAGCCA GCGGGTGCCT GTGAATATAG CTACTGTGAG 
GATGAGTCAG CCACAGGCGG ATGTCCCTTT GGGCCCTACC AGGGCCGCCA GACAAGCAGC 
ATCTTTGAGG CTGCAAAGAG AGAGCTAGCC AAGCTGATGC GGATTGAGGA CCCCTCTCTA 
CTGAACAGCA GAGTCTTGCT ACATCATGCC AAAGCCGGCA CCATCATAGC CCGCCAAGGG 
GACCAGGATG TGAGCCTGCA CTTTGTGCTC TGGGGCTGCC TGCACGTGTA CCAGCGCATG 
ATTGACAAGG CTGAGGAAGT GTGCCTATTT GTGGCACAGC CAGGGGAGCT GGTGGGGCAA 
CTGGCGGTGC TCACTGGGGA ACCTCTCATC TTCACACTAC GTGCCCAGAG AGACTGCACC 
TTCCTGCGGA TCTCCAAATC CCACTTCTAT GAGATCATGC GTGCACAACC CAGTGTGGTA 
CTGAGTGCAG CTCACACGGT GGCTGCTAGA ATGTCCCCCT TCGTGCGCCA GATGGACTTT 
GCCATTGACT GGACAGCTGT GGAGGCTGGT CGTGCGCTCT ACAGGCAGGG AGACCGCTCC 
GACTGCACCT ACATTGTACT CAATGGACGT CTCCGTAGTG TCATCCAACG AGGCAGTGGC 
AAGAAGGAGC TAGTTGGGGA GTATGGTCGA GGGGATCTCA TTGGCGTGGT GGAGGCACTG 
ACCCGGCAGC CACGAGCCAC CACTGTACAC GCAGTGCGAG ACACTGAGCT GGCCAAGCTT 
CCCGAGGGCA CCTTAGGCCA CATCAAACGT CGGTACCCAC AGGTTGTGAC CCGCCTTATT 
CATCTGCTAA GCCAGAAAAT TCTAGGCAAT CTGCAGCAGT TGCAAGGACC CTTTCCAGGC 
TCCGGGCTCA GTGTTCCGCA GCACTCGGAA CTGACCAACC CAGCCAGCAA TCTGTCTACT 
GTAGCTATCC TCCCTGTGTG TGCTGAGGTG CCCATGATGG CCTTTACTCT GGAACTGCAG 
CATGCTCTGC AAGCTATTGG TCCCACGCTC CTCCTTAACA GTGACGTCAT CCGGGCACTC 
CTGGGGGCTT CAGCACTGGA TAGCATTCAA GAATTCCGGC TGTCAGGGTG GTTGGCCCAG 
CAGGAAGATG CCCATCGCAT TGTGCTCTAC CAAACTGACA CATCCCTGAC GCCTTGGACC 
GTCCGTTGTC TGCGCCAGGC CGACTGCATC CTCATTGTGG GCCTGGGGGA CCAGGAACCC 
ACTGTTGGCC AGCTGGAGCA AATGTTAGAG AACACTGCTG TCCGTGCCTT GAAGCAACTG 
GTTTTGCTGC ACCGGGAGGA AGGCCCTGGT CCCACGCGCA CTGTAGAGTG GCTCAACATG 
CGCAGCTGGT GCTCAGGGCA CCTGCACCTG CGCTGTCCTC GCCGCCTCTT CTCACGGCGC 
AGCCCAGCGA AACTTCATGA GCTGTATGAG AAGGTTTTCT CCAGGCGTGC AGACCGTCAC 
AGCGACTTCT CCCGCTTGGC ACGAGTACTC ACAGGAAACA CTATTGCTCT GGTGCTGGGT 
GGGGGCGGAG CCAGAGGCTG CTCGCATATT GGGGTGCTGA AGGCATTAGA GGAGGCAGGA 
GTCCCAGTCG ACCTTGTGGG AGGCACATCC ATAGGTTCCT TCATTGGGGC CTTGTATGCC 
GAGGAACGCA GCGCCAGCCG AACTAAACAA CGAGCCCGGG AGTGGGCCAA GAGCATGACT 
TCTGTACTGG AGCCTGTATT GGACCTCACA TATCCTGTCA CCTCCATGTT TACTGGCTCA 
GCCTTTAACA GAAGTATCCA CCGTGTCTTC CAGGATAAGC AGATTGAGGA CCTGTGGCTG 
CCTTACTTCA ATGTGACCAC AGACATCACT GCCTCTGCCA TGCGCGTCCA CAAAGATGGC 
TCCCTGTGGC GGTATGTACG TGCCAGCATG ACGCTCTCGG GCTACCTACC CCCGCTGTGC 
GACCCAAAGG ATGGGCACCT GCTCATGGAT GGTGGCTACA TCAACAACCT GCCAGCGGAT 
ATTGCCCGAA GCATGGGAGC CAAAACGGTC ATTGCCATCG ACGTTGGAAG CCAGGATGAG 
ACAGATCTCA GCACCTATGG GGACAGCCTG TCTGGCTGGT GGTTGCTGTG GAAAAGGCTA 
AACCCCTGGG CAGACAAGGT AAAGGTTCCA GACATGGCTG AGATCCAGTC CCGCTTGGCG 
TACGTGTCCT GTGTGCGGCA GCTAGAAGTC GTCAAGTCCA GCTCCTATTG CGAGTACCTT 
CGTCCATCCA TTGACTGCTT CAAGACCATG GACTTTGGAA AGTTTGACCA GATCTATGAT 
GTGGGTTACC AGTATGGGAA GGCTGTCTTT GGAGGCTGGA CCCGTGGTGA AGTCATTGAG 
AAAATGCTCA CAGACCGGAG ATCTACAGAC CTTAATGAGA GTCGCCGTGC AGATATACTT 
GCCTTCCCAA GTTCTGGGTT CACTGACTTG GCTGAGATTG TGTCCCGGAT CGAGCCACCA 
ACAAGCTACG TCTCTGATGG CTGTGCTGAT GGGGAGGAGT CGGATTGCTT GACAGAGTAT 
GAGGAAGATG CAGGACCAGA CTGCTCAAGA GATGAAGGGG GCTCCCCTGA GGGTGCCAGC 
CCTAGCACTG CCTCAGAGGT GGAAGAAGAG AAGTCCACAC TCCGGCAACG ACGCTTTCTG 
CCTCAGGAGA CTCCCAGCTC AGTCGCAGAT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508888.3
CDS	82..4137
Translation	MEVVNLTIFQDCRLQDLLPSPKQSMEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508825.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508825.4

ORF Insert Sequence:

ATGGAAGTAG TCAATCTAAC AATTTTCCAG GACTGCAGGC TACAAGACCT GCTACCTAGT 
CCGAAACAAT CGATGGAGGC CCCACTGCAA ACCGGAATGG TGCTGGGCGT GATGATCGGG 
GCCGGAGTGG CGGTGCTGGT CACCGCCGTG CTCATCCTCT TGGTGGTGCG AAGGCTGCGG 
GTGCAGAAAA CTCCAGCCCC GGAGGGTCCC CGGTATCGGT TCCGGAAGCG GGACAAAGTG 
CTTTTCTATG GCCGGAAGAT TATGCGGAAG GTGTCACAGT CCACTTCTTC CCTGGTGGAC 
ACATCAGTCT CCACCACTTC CCGGCCCCGC ATGAAGAAGA AACTGAAGAT GCTTAACATT 
GCCAAGAAGA TCTTACGTAT CCAAAAGGAG ACTCCGACCC TACAACGGAA GGAGCCCCCA 
CCTTCAGTGC TGGAGGCTGA CCTCACAGAG GGAGACCTGG CCAATTCCCA CCTACCCTCT 
GAGGTACTCT ACATGCTTAA GAATGTTCGG GTGCTAGGTC ACTTTGAGAA GCCGCTCTTC 
CTGGAGCTCT GCCGGCACAT GGTCTTCCAG CGTCTCGGGC AGGGGGACTA TGTTTTTCGG 
CCCGGTCAAC CAGATGCCAG CATCTATGTG GTTCAAGATG GGCTGCTGGA GCTCTGCCTA 
CCAGGGCCTG ATGGGAAGGA GTGTGTGGTG AAGGAGGTGG TTCCTGGAGA CAGCGTCAAC 
AGCCTTCTGA GCATCCTTGA TGTCATCACG GGTCACCAGC ATCCCCAGCG GACTGTGTCA 
GCCAGGGCTG CACGGGACTC CACAGTGCTG AGACTCCCAG TAGAAGCCTT CTCTGCTGTC 
TTCACCAAGT ACCCTGAGAG TTTGGTGCGA GTGGTACAGA TCATCATGGT AAGGCTGCAG 
AGAGTCACCT TCCTGGCGCT TCACAATTAC CTGGGTCTGA CCAATGAACT CTTCAGTCAT 
GAGATCCAGC CCCTACGCCT TTTCCCCAGC CCCGGCCTCC CGACCCGAAC CAGCCCTGTC 
CGTGGCTCTA AACGGGTAGT CAGCACCTCA GGTACTGAGG ACACCTCAAA GGAGACCTCT 
GGCCGGCCCC TGGACTCCAT TGGAGCTCCT CTGCCTGGAC CTGCAGGGGA TCCCGTGAAG 
CCCACATCCT TAGAAGCACC CCCAGCCCCG TTACTGAGCC GCTGCATCTC CATGCCAGTA 
GACATCTCAG GCTTGCAAGG TGGCCCTCGT TCTGATTTTG ACATGGCCTA TGAACGTGGA 
CGGATATCTG TTTCTCTTCA AGAAGAGGCA TCTGGGGGAC CTCAGACAGC ATCCCCTAGG 
ACCCCCACTC AGGAGCTCCG GGAGCAGCCA GCGGGTGCCT GTGAATATAG CTACTGTGAG 
GATGAGTCAG CCACAGGCGG ATGTCCCTTT GGGCCCTACC AGGGCCGCCA GACAAGCAGC 
ATCTTTGAGG CTGCAAAGAG AGAGCTAGCC AAGCTGATGC GGATTGAGGA CCCCTCTCTA 
CTGAACAGCA GAGTCTTGCT ACATCATGCC AAAGCCGGCA CCATCATAGC CCGCCAAGGG 
GACCAGGATG TGAGCCTGCA CTTTGTGCTC TGGGGCTGCC TGCACGTGTA CCAGCGCATG 
ATTGACAAGG CTGAGGAAGT GTGCCTATTT GTGGCACAGC CAGGGGAGCT GGTGGGGCAA 
CTGGCGGTGC TCACTGGGGA ACCTCTCATC TTCACACTAC GTGCCCAGAG AGACTGCACC 
TTCCTGCGGA TCTCCAAATC CCACTTCTAT GAGATCATGC GTGCACAACC CAGTGTGGTA 
CTGAGTGCAG CTCACACGGT GGCTGCTAGA ATGTCCCCCT TCGTGCGCCA GATGGACTTT 
GCCATTGACT GGACAGCTGT GGAGGCTGGT CGTGCGCTCT ACAGGCAGGG AGACCGCTCC 
GACTGCACCT ACATTGTACT CAATGGACGT CTCCGTAGTG TCATCCAACG AGGCAGTGGC 
AAGAAGGAGC TAGTTGGGGA GTATGGTCGA GGGGATCTCA TTGGCGTGGT GGAGGCACTG 
ACCCGGCAGC CACGAGCCAC CACTGTACAC GCAGTGCGAG ACACTGAGCT GGCCAAGCTT 
CCCGAGGGCA CCTTAGGCCA CATCAAACGT CGGTACCCAC AGGTTGTGAC CCGCCTTATT 
CATCTGCTAA GCCAGAAAAT TCTAGGCAAT CTGCAGCAGT TGCAAGGACC CTTTCCAGGC 
TCCGGGCTCA GTGTTCCGCA GCACTCGGAA CTGACCAACC CAGCCAGCAA TCTGTCTACT 
GTAGCTATCC TCCCTGTGTG TGCTGAGGTG CCCATGATGG CCTTTACTCT GGAACTGCAG 
CATGCTCTGC AAGCTATTGG TCCCACGCTC CTCCTTAACA GTGACGTCAT CCGGGCACTC 
CTGGGGGCTT CAGCACTGGA TAGCATTCAA GAATTCCGGC TGTCAGGGTG GTTGGCCCAG 
CAGGAAGATG CCCATCGCAT TGTGCTCTAC CAAACTGACA CATCCCTGAC GCCTTGGACC 
GTCCGTTGTC TGCGCCAGGC CGACTGCATC CTCATTGTGG GCCTGGGGGA CCAGGAACCC 
ACTGTTGGCC AGCTGGAGCA AATGTTAGAG AACACTGCTG TCCGTGCCTT GAAGCAACTG 
GTTTTGCTGC ACCGGGAGGA AGGCCCTGGT CCCACGCGCA CTGTAGAGTG GCTCAACATG 
CGCAGCTGGT GCTCAGGGCA CCTGCACCTG CGCTGTCCTC GCCGCCTCTT CTCACGGCGC 
AGCCCAGCGA AACTTCATGA GCTGTATGAG AAGGTTTTCT CCAGGCGTGC AGACCGTCAC 
AGCGACTTCT CCCGCTTGGC ACGAGTACTC ACAGGAAACA CTATTGCTCT GGTGCTGGGT 
GGGGGCGGAG CCAGAGGCTG CTCGCATATT GGGGTGCTGA AGGCATTAGA GGAGGCAGGA 
GTCCCAGTCG ACCTTGTGGG AGGCACATCC ATAGGTTCCT TCATTGGGGC CTTGTATGCC 
GAGGAACGCA GCGCCAGCCG AACTAAACAA CGAGCCCGGG AGTGGGCCAA GAGCATGACT 
TCTGTACTGG AGCCTGTATT GGACCTCACA TATCCTGTCA CCTCCATGTT TACTGGCTCA 
GCCTTTAACA GAAGTATCCA CCGTGTCTTC CAGGATAAGC AGATTGAGGA CCTGTGGCTG 
CCTTACTTCA ATGTGACCAC AGACATCACT GCCTCTGCCA TGCGCGTCCA CAAAGATGGC 
TCCCTGTGGC GGTATGTACG TGCCAGCATG ACGCTCTCGG GCTACCTACC CCCGCTGTGC 
GACCCAAAGG ATGGGCACCT GCTCATGGAT GGTGGCTACA TCAACAACCT GCCAGCGGAT 
ATTGCCCGAA GCATGGGAGC CAAAACGGTC ATTGCCATCG ACGTTGGAAG CCAGGATGAG 
ACAGATCTCA GCACCTATGG GGACAGCCTG TCTGGCTGGT GGTTGCTGTG GAAAAGGCTA 
AACCCCTGGG CAGACAAGGT AAAGGTTCCA GACATGGCTG AGATCCAGTC CCGCTTGGCG 
TACGTGTCCT GTGTGCGGCA GCTAGAAGTC GTCAAGTCCA GCTCCTATTG CGAGTACCTT 
CGTCCATCCA TTGACTGCTT CAAGACCATG GACTTTGGAA AGTTTGACCA GATCTATGAT 
GTGGGTTACC AGTATGGGAA GGCTGTCTTT GGAGGCTGGA CCCGTGGTGA AGTCATTGAG 
AAAATGCTCA CAGACCGGAG ATCTACAGAC CTTAATGAGA GTCGCCGTGC AGATATACTT 
GCCTTCCCAA GTTCTGGGTT CACTGACTTG GCTGAGATTG TGTCCCGGAT CGAGCCACCA 
ACAAGCTACG TCTCTGATGG CTGTGCTGAT GGGGAGGAGT CGGATTGCTT GACAGAGTAT 
GAGGAAGATG CAGGACCAGA CTGCTCAAGA GATGAAGGGG GCTCCCCTGA GGGTGCCAGC 
CCTAGCACTG CCTCAGAGGT GGAAGAAGAG AAGTCCACAC TCCGGCAACG ACGCTTTCTG 
CCTCAGGAGA CTCCCAGCTC AGTCGCAGAT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508827.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508890.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X3, mRNA.

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CloneID	OMu40426
Clone ID Related Accession (Same CDS sequence)	XM_006508826.3 , XM_006508829.1 , XM_006508827.4 , XM_006508829.2 , XM_006508827.3 , NM_001359122.1 , XM_006508828.1
Accession Version	XM_006508827.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508827.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGACCCCC 
ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG 
TCAGCCACAG GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT 
GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC 
AGCAGAGTCT TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG 
GATGTGAGCC TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC 
AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG 
GTGCTCACTG GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG 
CGGATCTCCA AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT 
GCAGCTCACA CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT 
GACTGGACAG CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC 
ACCTACATTG TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG 
GAGCTAGTTG GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG 
CAGCCACGAG CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG 
GGCACCTTAG GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG 
CTAAGCCAGA AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG 
CTCAGTGTTC CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT 
ATCCTCCCTG TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT 
CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG 
GCTTCAGCAC TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA 
GATGCCCATC GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT 
TGTCTGCGCC AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT 
GGCCAGCTGG AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG 
CTGCACCGGG AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC 
TGGTGCTCAG GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA 
GCGAAACTTC ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC 
TTCTCCCGCT TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC 
GGAGCCAGAG GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA 
GTCGACCTTG TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA 
CGCAGCGCCA GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA 
CTGGAGCCTG TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT 
AACAGAAGTA TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC 
TTCAATGTGA CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG 
TGGCGGTATG TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA 
AAGGATGGGC ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC 
CGAAGCATGG GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT 
CTCAGCACCT ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC 
TGGGCAGACA AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG 
TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA 
TCCATTGACT GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT 
TACCAGTATG GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG 
CTCACAGACC GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC 
CCAAGTTCTG GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC 
TACGTCTCTG ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA 
GATGCAGGAC CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC 
ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG 
GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508890.1
CDS	358..4407
Translation	MEESLQPKDCRLQDLLPSPKQSMEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508827.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508827.4

ORF Insert Sequence:

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGACCCCC 
ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG 
TCAGCCACAG GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT 
GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC 
AGCAGAGTCT TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG 
GATGTGAGCC TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC 
AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG 
GTGCTCACTG GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG 
CGGATCTCCA AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT 
GCAGCTCACA CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT 
GACTGGACAG CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC 
ACCTACATTG TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG 
GAGCTAGTTG GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG 
CAGCCACGAG CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG 
GGCACCTTAG GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG 
CTAAGCCAGA AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG 
CTCAGTGTTC CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT 
ATCCTCCCTG TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT 
CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG 
GCTTCAGCAC TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA 
GATGCCCATC GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT 
TGTCTGCGCC AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT 
GGCCAGCTGG AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG 
CTGCACCGGG AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC 
TGGTGCTCAG GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA 
GCGAAACTTC ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC 
TTCTCCCGCT TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC 
GGAGCCAGAG GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA 
GTCGACCTTG TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA 
CGCAGCGCCA GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA 
CTGGAGCCTG TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT 
AACAGAAGTA TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC 
TTCAATGTGA CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG 
TGGCGGTATG TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA 
AAGGATGGGC ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC 
CGAAGCATGG GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT 
CTCAGCACCT ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC 
TGGGCAGACA AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG 
TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA 
TCCATTGACT GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT 
TACCAGTATG GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG 
CTCACAGACC GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC 
CCAAGTTCTG GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC 
TACGTCTCTG ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA 
GATGCAGGAC CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC 
ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG 
GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508829.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508892.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X5, mRNA.

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CloneID	OMu40426
Clone ID Related Accession (Same CDS sequence)	XM_006508826.3 , XM_006508829.1 , XM_006508827.4 , XM_006508829.2 , XM_006508827.3 , NM_001359122.1 , XM_006508828.1
Accession Version	XM_006508829.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508829.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGACCCCC 
ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG 
TCAGCCACAG GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT 
GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC 
AGCAGAGTCT TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG 
GATGTGAGCC TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC 
AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG 
GTGCTCACTG GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG 
CGGATCTCCA AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT 
GCAGCTCACA CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT 
GACTGGACAG CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC 
ACCTACATTG TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG 
GAGCTAGTTG GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG 
CAGCCACGAG CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG 
GGCACCTTAG GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG 
CTAAGCCAGA AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG 
CTCAGTGTTC CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT 
ATCCTCCCTG TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT 
CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG 
GCTTCAGCAC TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA 
GATGCCCATC GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT 
TGTCTGCGCC AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT 
GGCCAGCTGG AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG 
CTGCACCGGG AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC 
TGGTGCTCAG GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA 
GCGAAACTTC ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC 
TTCTCCCGCT TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC 
GGAGCCAGAG GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA 
GTCGACCTTG TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA 
CGCAGCGCCA GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA 
CTGGAGCCTG TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT 
AACAGAAGTA TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC 
TTCAATGTGA CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG 
TGGCGGTATG TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA 
AAGGATGGGC ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC 
CGAAGCATGG GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT 
CTCAGCACCT ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC 
TGGGCAGACA AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG 
TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA 
TCCATTGACT GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT 
TACCAGTATG GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG 
CTCACAGACC GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC 
CCAAGTTCTG GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC 
TACGTCTCTG ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA 
GATGCAGGAC CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC 
ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG 
GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508892.1
CDS	82..4131
Translation	MEESLQPKDCRLQDLLPSPKQSMEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508829.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508829.2

ORF Insert Sequence:

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGACCCCC 
ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG 
TCAGCCACAG GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT 
GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC 
AGCAGAGTCT TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG 
GATGTGAGCC TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC 
AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG 
GTGCTCACTG GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG 
CGGATCTCCA AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT 
GCAGCTCACA CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT 
GACTGGACAG CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC 
ACCTACATTG TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG 
GAGCTAGTTG GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG 
CAGCCACGAG CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG 
GGCACCTTAG GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG 
CTAAGCCAGA AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG 
CTCAGTGTTC CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT 
ATCCTCCCTG TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT 
CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG 
GCTTCAGCAC TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA 
GATGCCCATC GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT 
TGTCTGCGCC AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT 
GGCCAGCTGG AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG 
CTGCACCGGG AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC 
TGGTGCTCAG GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA 
GCGAAACTTC ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC 
TTCTCCCGCT TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC 
GGAGCCAGAG GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA 
GTCGACCTTG TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA 
CGCAGCGCCA GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA 
CTGGAGCCTG TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT 
AACAGAAGTA TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC 
TTCAATGTGA CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG 
TGGCGGTATG TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA 
AAGGATGGGC ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC 
CGAAGCATGG GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT 
CTCAGCACCT ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC 
TGGGCAGACA AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG 
TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA 
TCCATTGACT GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT 
TACCAGTATG GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG 
CTCACAGACC GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC 
CCAAGTTCTG GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC 
TACGTCTCTG ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA 
GATGCAGGAC CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC 
ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG 
GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508830.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508893.3 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X6, mRNA.

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CloneID	OMu40427
Clone ID Related Accession (Same CDS sequence)	XM_006508830.4
Accession Version	XM_006508830.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508830.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAG TCAATCTAAC AATTTTCCAG GACTGCAGGC TACAAGACCT GCTACCTAGT 
CCGAAACAAT CGATGGAGGC CCCACTGCAA ACCGGAATGG TGCTGGGCGT GATGATCGGG 
GCCGGAGTGG CGGTGCTGGT CACCGCCGTG CTCATCCTCT TGGTGGTGCG AAGGCTGCGG 
GTGCAGAAAA CTCCAGCCCC GGAGGGTCCC CGGTATCGGT TCCGGAAGCG GGACAAAGTG 
CTTTTCTATG GCCGGAAGAT TATGCGGAAG GTGTCACAGT CCACTTCTTC CCTGGTGGAC 
ACATCAGTCT CCACCACTTC CCGGCCCCGC ATGAAGAAGA AACTGAAGAT GCTTAACATT 
GCCAAGAAGA TCTTACGTAT CCAAAAGGAG ACTCCGACCC TACAACGGAA GGAGCCCCCA 
CCTTCAGTGC TGGAGGCTGA CCTCACAGAG GGAGACCTGG CCAATTCCCA CCTACCCTCT 
GAGGTACTCT ACATGCTTAA GAATGTTCGG GTGCTAGGTC ACTTTGAGAA GCCGCTCTTC 
CTGGAGCTCT GCCGGCACAT GGTCTTCCAG CGTCTCGGGC AGGGGGACTA TGTTTTTCGG 
CCCGGTCAAC CAGATGCCAG CATCTATGTG GTTCAAGATG GGCTGCTGGA GCTCTGCCTA 
CCAGGGCCTG ATGGGAAGGA GTGTGTGGTG AAGGAGGTGG TTCCTGGAGA CAGCGTCAAC 
AGCCTTCTGA GCATCCTTGA TGTCATCACG GGTCACCAGC ATCCCCAGCG GACTGTGTCA 
GCCAGGGCTG CACGGGACTC CACAGTGCTG AGACTCCCAG TAGAAGCCTT CTCTGCTGTC 
TTCACCAAGT ACCCTGAGAG TTTGGTGCGA GTGGTACAGA TCATCATGGT AAGGCTGCAG 
AGAGTCACCT TCCTGGCGCT TCACAATTAC CTGGGTCTGA CCAATGAACT CTTCAGTCAT 
GAGATCCAGC CCCTACGCCT TTTCCCCAGC CCCGGCCTCC CGACCCGAAC CAGCCCTGTC 
CGTGGCTCTA AACGGGTAGT CAGCACCTCA GGTACTGAGG ACACCTCAAA GGAGACCTCT 
GGCCGGCCCC TGGACTCCAT TGGAGCTCCT CTGCCTGGAC CTGCAGGGGA TCCCGTGAAG 
CCCACATCCT TAGAAGCACC CCCAGCCCCG TTACTGAGCC GCTGCATCTC CATGCCAGTA 
GACATCTCAG GCTTGCAAGG TGGCCCTCGT TCTGATTTTG ACATGGCCTA TGAACGTGGA 
CGGATATCTG TTTCTCTTCA AGAAGAGGCA TCTGGGGGAC CTCAGACAGC ATCCCCTAGG 
GAGCTCCGGG AGCAGCCAGC GGGTGCCTGT GAATATAGCT ACTGTGAGGA TGAGTCAGCC 
ACAGGCGGAT GTCCCTTTGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCT 
GCAAAGAGAG AGCTAGCCAA GCTGATGCGG ATTGAGGACC CCTCTCTACT GAACAGCAGA 
GTCTTGCTAC ATCATGCCAA AGCCGGCACC ATCATAGCCC GCCAAGGGGA CCAGGATGTG 
AGCCTGCACT TTGTGCTCTG GGGCTGCCTG CACGTGTACC AGCGCATGAT TGACAAGGCT 
GAGGAAGTGT GCCTATTTGT GGCACAGCCA GGGGAGCTGG TGGGGCAACT GGCGGTGCTC 
ACTGGGGAAC CTCTCATCTT CACACTACGT GCCCAGAGAG ACTGCACCTT CCTGCGGATC 
TCCAAATCCC ACTTCTATGA GATCATGCGT GCACAACCCA GTGTGGTACT GAGTGCAGCT 
CACACGGTGG CTGCTAGAAT GTCCCCCTTC GTGCGCCAGA TGGACTTTGC CATTGACTGG 
ACAGCTGTGG AGGCTGGTCG TGCGCTCTAC AGGCAGGGAG ACCGCTCCGA CTGCACCTAC 
ATTGTACTCA ATGGACGTCT CCGTAGTGTC ATCCAACGAG GCAGTGGCAA GAAGGAGCTA 
GTTGGGGAGT ATGGTCGAGG GGATCTCATT GGCGTGGTGG AGGCACTGAC CCGGCAGCCA 
CGAGCCACCA CTGTACACGC AGTGCGAGAC ACTGAGCTGG CCAAGCTTCC CGAGGGCACC 
TTAGGCCACA TCAAACGTCG GTACCCACAG GTTGTGACCC GCCTTATTCA TCTGCTAAGC 
CAGAAAATTC TAGGCAATCT GCAGCAGTTG CAAGGACCCT TTCCAGGCTC CGGGCTCAGT 
GTTCCGCAGC ACTCGGAACT GACCAACCCA GCCAGCAATC TGTCTACTGT AGCTATCCTC 
CCTGTGTGTG CTGAGGTGCC CATGATGGCC TTTACTCTGG AACTGCAGCA TGCTCTGCAA 
GCTATTGGTC CCACGCTCCT CCTTAACAGT GACGTCATCC GGGCACTCCT GGGGGCTTCA 
GCACTGGATA GCATTCAAGA ATTCCGGCTG TCAGGGTGGT TGGCCCAGCA GGAAGATGCC 
CATCGCATTG TGCTCTACCA AACTGACACA TCCCTGACGC CTTGGACCGT CCGTTGTCTG 
CGCCAGGCCG ACTGCATCCT CATTGTGGGC CTGGGGGACC AGGAACCCAC TGTTGGCCAG 
CTGGAGCAAA TGTTAGAGAA CACTGCTGTC CGTGCCTTGA AGCAACTGGT TTTGCTGCAC 
CGGGAGGAAG GCCCTGGTCC CACGCGCACT GTAGAGTGGC TCAACATGCG CAGCTGGTGC 
TCAGGGCACC TGCACCTGCG CTGTCCTCGC CGCCTCTTCT CACGGCGCAG CCCAGCGAAA 
CTTCATGAGC TGTATGAGAA GGTTTTCTCC AGGCGTGCAG ACCGTCACAG CGACTTCTCC 
CGCTTGGCAC GAGTACTCAC AGGAAACACT ATTGCTCTGG TGCTGGGTGG GGGCGGAGCC 
AGAGGCTGCT CGCATATTGG GGTGCTGAAG GCATTAGAGG AGGCAGGAGT CCCAGTCGAC 
CTTGTGGGAG GCACATCCAT AGGTTCCTTC ATTGGGGCCT TGTATGCCGA GGAACGCAGC 
GCCAGCCGAA CTAAACAACG AGCCCGGGAG TGGGCCAAGA GCATGACTTC TGTACTGGAG 
CCTGTATTGG ACCTCACATA TCCTGTCACC TCCATGTTTA CTGGCTCAGC CTTTAACAGA 
AGTATCCACC GTGTCTTCCA GGATAAGCAG ATTGAGGACC TGTGGCTGCC TTACTTCAAT 
GTGACCACAG ACATCACTGC CTCTGCCATG CGCGTCCACA AAGATGGCTC CCTGTGGCGG 
TATGTACGTG CCAGCATGAC GCTCTCGGGC TACCTACCCC CGCTGTGCGA CCCAAAGGAT 
GGGCACCTGC TCATGGATGG TGGCTACATC AACAACCTGC CAGCGGATAT TGCCCGAAGC 
ATGGGAGCCA AAACGGTCAT TGCCATCGAC GTTGGAAGCC AGGATGAGAC AGATCTCAGC 
ACCTATGGGG ACAGCCTGTC TGGCTGGTGG TTGCTGTGGA AAAGGCTAAA CCCCTGGGCA 
GACAAGGTAA AGGTTCCAGA CATGGCTGAG ATCCAGTCCC GCTTGGCGTA CGTGTCCTGT 
GTGCGGCAGC TAGAAGTCGT CAAGTCCAGC TCCTATTGCG AGTACCTTCG TCCATCCATT 
GACTGCTTCA AGACCATGGA CTTTGGAAAG TTTGACCAGA TCTATGATGT GGGTTACCAG 
TATGGGAAGG CTGTCTTTGG AGGCTGGACC CGTGGTGAAG TCATTGAGAA AATGCTCACA 
GACCGGAGAT CTACAGACCT TAATGAGAGT CGCCGTGCAG ATATACTTGC CTTCCCAAGT 
TCTGGGTTCA CTGACTTGGC TGAGATTGTG TCCCGGATCG AGCCACCAAC AAGCTACGTC 
TCTGATGGCT GTGCTGATGG GGAGGAGTCG GATTGCTTGA CAGAGTATGA GGAAGATGCA 
GGACCAGACT GCTCAAGAGA TGAAGGGGGC TCCCCTGAGG GTGCCAGCCC TAGCACTGCC 
TCAGAGGTGG AAGAAGAGAA GTCCACACTC CGGCAACGAC GCTTTCTGCC TCAGGAGACT 
CCCAGCTCAG TCGCAGATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508893.3
CDS	82..4125
Translation	MEVVNLTIFQDCRLQDLLPSPKQSMEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508830.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508830.4

ORF Insert Sequence:

ATGGAAGTAG TCAATCTAAC AATTTTCCAG GACTGCAGGC TACAAGACCT GCTACCTAGT 
CCGAAACAAT CGATGGAGGC CCCACTGCAA ACCGGAATGG TGCTGGGCGT GATGATCGGG 
GCCGGAGTGG CGGTGCTGGT CACCGCCGTG CTCATCCTCT TGGTGGTGCG AAGGCTGCGG 
GTGCAGAAAA CTCCAGCCCC GGAGGGTCCC CGGTATCGGT TCCGGAAGCG GGACAAAGTG 
CTTTTCTATG GCCGGAAGAT TATGCGGAAG GTGTCACAGT CCACTTCTTC CCTGGTGGAC 
ACATCAGTCT CCACCACTTC CCGGCCCCGC ATGAAGAAGA AACTGAAGAT GCTTAACATT 
GCCAAGAAGA TCTTACGTAT CCAAAAGGAG ACTCCGACCC TACAACGGAA GGAGCCCCCA 
CCTTCAGTGC TGGAGGCTGA CCTCACAGAG GGAGACCTGG CCAATTCCCA CCTACCCTCT 
GAGGTACTCT ACATGCTTAA GAATGTTCGG GTGCTAGGTC ACTTTGAGAA GCCGCTCTTC 
CTGGAGCTCT GCCGGCACAT GGTCTTCCAG CGTCTCGGGC AGGGGGACTA TGTTTTTCGG 
CCCGGTCAAC CAGATGCCAG CATCTATGTG GTTCAAGATG GGCTGCTGGA GCTCTGCCTA 
CCAGGGCCTG ATGGGAAGGA GTGTGTGGTG AAGGAGGTGG TTCCTGGAGA CAGCGTCAAC 
AGCCTTCTGA GCATCCTTGA TGTCATCACG GGTCACCAGC ATCCCCAGCG GACTGTGTCA 
GCCAGGGCTG CACGGGACTC CACAGTGCTG AGACTCCCAG TAGAAGCCTT CTCTGCTGTC 
TTCACCAAGT ACCCTGAGAG TTTGGTGCGA GTGGTACAGA TCATCATGGT AAGGCTGCAG 
AGAGTCACCT TCCTGGCGCT TCACAATTAC CTGGGTCTGA CCAATGAACT CTTCAGTCAT 
GAGATCCAGC CCCTACGCCT TTTCCCCAGC CCCGGCCTCC CGACCCGAAC CAGCCCTGTC 
CGTGGCTCTA AACGGGTAGT CAGCACCTCA GGTACTGAGG ACACCTCAAA GGAGACCTCT 
GGCCGGCCCC TGGACTCCAT TGGAGCTCCT CTGCCTGGAC CTGCAGGGGA TCCCGTGAAG 
CCCACATCCT TAGAAGCACC CCCAGCCCCG TTACTGAGCC GCTGCATCTC CATGCCAGTA 
GACATCTCAG GCTTGCAAGG TGGCCCTCGT TCTGATTTTG ACATGGCCTA TGAACGTGGA 
CGGATATCTG TTTCTCTTCA AGAAGAGGCA TCTGGGGGAC CTCAGACAGC ATCCCCTAGG 
GAGCTCCGGG AGCAGCCAGC GGGTGCCTGT GAATATAGCT ACTGTGAGGA TGAGTCAGCC 
ACAGGCGGAT GTCCCTTTGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCT 
GCAAAGAGAG AGCTAGCCAA GCTGATGCGG ATTGAGGACC CCTCTCTACT GAACAGCAGA 
GTCTTGCTAC ATCATGCCAA AGCCGGCACC ATCATAGCCC GCCAAGGGGA CCAGGATGTG 
AGCCTGCACT TTGTGCTCTG GGGCTGCCTG CACGTGTACC AGCGCATGAT TGACAAGGCT 
GAGGAAGTGT GCCTATTTGT GGCACAGCCA GGGGAGCTGG TGGGGCAACT GGCGGTGCTC 
ACTGGGGAAC CTCTCATCTT CACACTACGT GCCCAGAGAG ACTGCACCTT CCTGCGGATC 
TCCAAATCCC ACTTCTATGA GATCATGCGT GCACAACCCA GTGTGGTACT GAGTGCAGCT 
CACACGGTGG CTGCTAGAAT GTCCCCCTTC GTGCGCCAGA TGGACTTTGC CATTGACTGG 
ACAGCTGTGG AGGCTGGTCG TGCGCTCTAC AGGCAGGGAG ACCGCTCCGA CTGCACCTAC 
ATTGTACTCA ATGGACGTCT CCGTAGTGTC ATCCAACGAG GCAGTGGCAA GAAGGAGCTA 
GTTGGGGAGT ATGGTCGAGG GGATCTCATT GGCGTGGTGG AGGCACTGAC CCGGCAGCCA 
CGAGCCACCA CTGTACACGC AGTGCGAGAC ACTGAGCTGG CCAAGCTTCC CGAGGGCACC 
TTAGGCCACA TCAAACGTCG GTACCCACAG GTTGTGACCC GCCTTATTCA TCTGCTAAGC 
CAGAAAATTC TAGGCAATCT GCAGCAGTTG CAAGGACCCT TTCCAGGCTC CGGGCTCAGT 
GTTCCGCAGC ACTCGGAACT GACCAACCCA GCCAGCAATC TGTCTACTGT AGCTATCCTC 
CCTGTGTGTG CTGAGGTGCC CATGATGGCC TTTACTCTGG AACTGCAGCA TGCTCTGCAA 
GCTATTGGTC CCACGCTCCT CCTTAACAGT GACGTCATCC GGGCACTCCT GGGGGCTTCA 
GCACTGGATA GCATTCAAGA ATTCCGGCTG TCAGGGTGGT TGGCCCAGCA GGAAGATGCC 
CATCGCATTG TGCTCTACCA AACTGACACA TCCCTGACGC CTTGGACCGT CCGTTGTCTG 
CGCCAGGCCG ACTGCATCCT CATTGTGGGC CTGGGGGACC AGGAACCCAC TGTTGGCCAG 
CTGGAGCAAA TGTTAGAGAA CACTGCTGTC CGTGCCTTGA AGCAACTGGT TTTGCTGCAC 
CGGGAGGAAG GCCCTGGTCC CACGCGCACT GTAGAGTGGC TCAACATGCG CAGCTGGTGC 
TCAGGGCACC TGCACCTGCG CTGTCCTCGC CGCCTCTTCT CACGGCGCAG CCCAGCGAAA 
CTTCATGAGC TGTATGAGAA GGTTTTCTCC AGGCGTGCAG ACCGTCACAG CGACTTCTCC 
CGCTTGGCAC GAGTACTCAC AGGAAACACT ATTGCTCTGG TGCTGGGTGG GGGCGGAGCC 
AGAGGCTGCT CGCATATTGG GGTGCTGAAG GCATTAGAGG AGGCAGGAGT CCCAGTCGAC 
CTTGTGGGAG GCACATCCAT AGGTTCCTTC ATTGGGGCCT TGTATGCCGA GGAACGCAGC 
GCCAGCCGAA CTAAACAACG AGCCCGGGAG TGGGCCAAGA GCATGACTTC TGTACTGGAG 
CCTGTATTGG ACCTCACATA TCCTGTCACC TCCATGTTTA CTGGCTCAGC CTTTAACAGA 
AGTATCCACC GTGTCTTCCA GGATAAGCAG ATTGAGGACC TGTGGCTGCC TTACTTCAAT 
GTGACCACAG ACATCACTGC CTCTGCCATG CGCGTCCACA AAGATGGCTC CCTGTGGCGG 
TATGTACGTG CCAGCATGAC GCTCTCGGGC TACCTACCCC CGCTGTGCGA CCCAAAGGAT 
GGGCACCTGC TCATGGATGG TGGCTACATC AACAACCTGC CAGCGGATAT TGCCCGAAGC 
ATGGGAGCCA AAACGGTCAT TGCCATCGAC GTTGGAAGCC AGGATGAGAC AGATCTCAGC 
ACCTATGGGG ACAGCCTGTC TGGCTGGTGG TTGCTGTGGA AAAGGCTAAA CCCCTGGGCA 
GACAAGGTAA AGGTTCCAGA CATGGCTGAG ATCCAGTCCC GCTTGGCGTA CGTGTCCTGT 
GTGCGGCAGC TAGAAGTCGT CAAGTCCAGC TCCTATTGCG AGTACCTTCG TCCATCCATT 
GACTGCTTCA AGACCATGGA CTTTGGAAAG TTTGACCAGA TCTATGATGT GGGTTACCAG 
TATGGGAAGG CTGTCTTTGG AGGCTGGACC CGTGGTGAAG TCATTGAGAA AATGCTCACA 
GACCGGAGAT CTACAGACCT TAATGAGAGT CGCCGTGCAG ATATACTTGC CTTCCCAAGT 
TCTGGGTTCA CTGACTTGGC TGAGATTGTG TCCCGGATCG AGCCACCAAC AAGCTACGTC 
TCTGATGGCT GTGCTGATGG GGAGGAGTCG GATTGCTTGA CAGAGTATGA GGAAGATGCA 
GGACCAGACT GCTCAAGAGA TGAAGGGGGC TCCCCTGAGG GTGCCAGCCC TAGCACTGCC 
TCAGAGGTGG AAGAAGAGAA GTCCACACTC CGGCAACGAC GCTTTCTGCC TCAGGAGACT 
CCCAGCTCAG TCGCAGATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508832.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508895.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu40428
Clone ID Related Accession (Same CDS sequence)	XM_006508832.3 , XM_006508832.4
Accession Version	XM_006508832.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508832.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTGCC CAGCCTGTGC CGTTCGTTCC GCAGAAACTC CAGCCCCGGA GGGTCCCCGG 
TATCGGTTCC GGAAGCGGGA CAAAGTGCTT TTCTATGGCC GGAAGATTAT GCGGAAGGTG 
TCACAGTCCA CTTCTTCCCT GGTGGACACA TCAGTCTCCA CCACTTCCCG GCCCCGCATG 
AAGAAGAAAC TGAAGATGCT TAACATTGCC AAGAAGATCT TACGTATCCA AAAGGAGACT 
CCGACCCTAC AACGGAAGGA GCCCCCACCT TCAGTGCTGG AGGCTGACCT CACAGAGGGA 
GACCTGGCCA ATTCCCACCT ACCCTCTGAG GTACTCTACA TGCTTAAGAA TGTTCGGGTG 
CTAGGTCACT TTGAGAAGCC GCTCTTCCTG GAGCTCTGCC GGCACATGGT CTTCCAGCGT 
CTCGGGCAGG GGGACTATGT TTTTCGGCCC GGTCAACCAG ATGCCAGCAT CTATGTGGTT 
CAAGATGGGC TGCTGGAGCT CTGCCTACCA GGGCCTGATG GGAAGGAGTG TGTGGTGAAG 
GAGGTGGTTC CTGGAGACAG CGTCAACAGC CTTCTGAGCA TCCTTGATGT CATCACGGGT 
CACCAGCATC CCCAGCGGAC TGTGTCAGCC AGGGCTGCAC GGGACTCCAC AGTGCTGAGA 
CTCCCAGTAG AAGCCTTCTC TGCTGTCTTC ACCAAGTACC CTGAGAGTTT GGTGCGAGTG 
GTACAGATCA TCATGGTAAG GCTGCAGAGA GTCACCTTCC TGGCGCTTCA CAATTACCTG 
GGTCTGACCA ATGAACTCTT CAGTCATGAG ATCCAGCCCC TACGCCTTTT CCCCAGCCCC 
GGCCTCCCGA CCCGAACCAG CCCTGTCCGT GGCTCTAAAC GGGTAGTCAG CACCTCAGGT 
ACTGAGGACA CCTCAAAGGA GACCTCTGGC CGGCCCCTGG ACTCCATTGG AGCTCCTCTG 
CCTGGACCTG CAGGGGATCC CGTGAAGCCC ACATCCTTAG AAGCACCCCC AGCCCCGTTA 
CTGAGCCGCT GCATCTCCAT GCCAGTAGAC ATCTCAGGCT TGCAAGGTGG CCCTCGTTCT 
GATTTTGACA TGGCCTATGA ACGTGGACGG ATATCTGTTT CTCTTCAAGA AGAGGCATCT 
GGGGGACCTC AGACAGCATC CCCTAGGACC CCCACTCAGG AGCTCCGGGA GCAGCCAGCG 
GGTGCCTGTG AATATAGCTA CTGTGAGGAT GAGTCAGCCA CAGGCGGATG TCCCTTTGGG 
CCCTACCAGG GCCGCCAGAC AAGCAGCATC TTTGAGGCTG CAAAGAGAGA GCTAGCCAAG 
CTGATGCGGA TTGAGGACCC CTCTCTACTG AACAGCAGAG TCTTGCTACA TCATGCCAAA 
GCCGGCACCA TCATAGCCCG CCAAGGGGAC CAGGATGTGA GCCTGCACTT TGTGCTCTGG 
GGCTGCCTGC ACGTGTACCA GCGCATGATT GACAAGGCTG AGGAAGTGTG CCTATTTGTG 
GCACAGCCAG GGGAGCTGGT GGGGCAACTG GCGGTGCTCA CTGGGGAACC TCTCATCTTC 
ACACTACGTG CCCAGAGAGA CTGCACCTTC CTGCGGATCT CCAAATCCCA CTTCTATGAG 
ATCATGCGTG CACAACCCAG TGTGGTACTG AGTGCAGCTC ACACGGTGGC TGCTAGAATG 
TCCCCCTTCG TGCGCCAGAT GGACTTTGCC ATTGACTGGA CAGCTGTGGA GGCTGGTCGT 
GCGCTCTACA GGCAGGGAGA CCGCTCCGAC TGCACCTACA TTGTACTCAA TGGACGTCTC 
CGTAGTGTCA TCCAACGAGG CAGTGGCAAG AAGGAGCTAG TTGGGGAGTA TGGTCGAGGG 
GATCTCATTG GCGTGGTGGA GGCACTGACC CGGCAGCCAC GAGCCACCAC TGTACACGCA 
GTGCGAGACA CTGAGCTGGC CAAGCTTCCC GAGGGCACCT TAGGCCACAT CAAACGTCGG 
TACCCACAGG TTGTGACCCG CCTTATTCAT CTGCTAAGCC AGAAAATTCT AGGCAATCTG 
CAGCAGTTGC AAGGACCCTT TCCAGGCTCC GGGCTCAGTG TTCCGCAGCA CTCGGAACTG 
ACCAACCCAG CCAGCAATCT GTCTACTGTA GCTATCCTCC CTGTGTGTGC TGAGGTGCCC 
ATGATGGCCT TTACTCTGGA ACTGCAGCAT GCTCTGCAAG CTATTGGTCC CACGCTCCTC 
CTTAACAGTG ACGTCATCCG GGCACTCCTG GGGGCTTCAG CACTGGATAG CATTCAAGAA 
TTCCGGCTGT CAGGGTGGTT GGCCCAGCAG GAAGATGCCC ATCGCATTGT GCTCTACCAA 
ACTGACACAT CCCTGACGCC TTGGACCGTC CGTTGTCTGC GCCAGGCCGA CTGCATCCTC 
ATTGTGGGCC TGGGGGACCA GGAACCCACT GTTGGCCAGC TGGAGCAAAT GTTAGAGAAC 
ACTGCTGTCC GTGCCTTGAA GCAACTGGTT TTGCTGCACC GGGAGGAAGG CCCTGGTCCC 
ACGCGCACTG TAGAGTGGCT CAACATGCGC AGCTGGTGCT CAGGGCACCT GCACCTGCGC 
TGTCCTCGCC GCCTCTTCTC ACGGCGCAGC CCAGCGAAAC TTCATGAGCT GTATGAGAAG 
GTTTTCTCCA GGCGTGCAGA CCGTCACAGC GACTTCTCCC GCTTGGCACG AGTACTCACA 
GGAAACACTA TTGCTCTGGT GCTGGGTGGG GGCGGAGCCA GAGGCTGCTC GCATATTGGG 
GTGCTGAAGG CATTAGAGGA GGCAGGAGTC CCAGTCGACC TTGTGGGAGG CACATCCATA 
GGTTCCTTCA TTGGGGCCTT GTATGCCGAG GAACGCAGCG CCAGCCGAAC TAAACAACGA 
GCCCGGGAGT GGGCCAAGAG CATGACTTCT GTACTGGAGC CTGTATTGGA CCTCACATAT 
CCTGTCACCT CCATGTTTAC TGGCTCAGCC TTTAACAGAA GTATCCACCG TGTCTTCCAG 
GATAAGCAGA TTGAGGACCT GTGGCTGCCT TACTTCAATG TGACCACAGA CATCACTGCC 
TCTGCCATGC GCGTCCACAA AGATGGCTCC CTGTGGCGGT ATGTACGTGC CAGCATGACG 
CTCTCGGGCT ACCTACCCCC GCTGTGCGAC CCAAAGGATG GGCACCTGCT CATGGATGGT 
GGCTACATCA ACAACCTGCC AGCGGATATT GCCCGAAGCA TGGGAGCCAA AACGGTCATT 
GCCATCGACG TTGGAAGCCA GGATGAGACA GATCTCAGCA CCTATGGGGA CAGCCTGTCT 
GGCTGGTGGT TGCTGTGGAA AAGGCTAAAC CCCTGGGCAG ACAAGGTAAA GGTTCCAGAC 
ATGGCTGAGA TCCAGTCCCG CTTGGCGTAC GTGTCCTGTG TGCGGCAGCT AGAAGTCGTC 
AAGTCCAGCT CCTATTGCGA GTACCTTCGT CCATCCATTG ACTGCTTCAA GACCATGGAC 
TTTGGAAAGT TTGACCAGAT CTATGATGTG GGTTACCAGT ATGGGAAGGC TGTCTTTGGA 
GGCTGGACCC GTGGTGAAGT CATTGAGAAA ATGCTCACAG ACCGGAGATC TACAGACCTT 
AATGAGAGTC GCCGTGCAGA TATACTTGCC TTCCCAAGTT CTGGGTTCAC TGACTTGGCT 
GAGATTGTGT CCCGGATCGA GCCACCAACA AGCTACGTCT CTGATGGCTG TGCTGATGGG 
GAGGAGTCGG ATTGCTTGAC AGAGTATGAG GAAGATGCAG GACCAGACTG CTCAAGAGAT 
GAAGGGGGCT CCCCTGAGGG TGCCAGCCCT AGCACTGCCT CAGAGGTGGA AGAAGAGAAG 
TCCACACTCC GGCAACGACG CTTTCTGCCT CAGGAGACTC CCAGCTCAGT CGCAGATGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508895.1
CDS	231..4133
Translation	MRCPACAVRSAETPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508832.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508832.4

ORF Insert Sequence:

ATGCGTTGCC CAGCCTGTGC CGTTCGTTCC GCAGAAACTC CAGCCCCGGA GGGTCCCCGG 
TATCGGTTCC GGAAGCGGGA CAAAGTGCTT TTCTATGGCC GGAAGATTAT GCGGAAGGTG 
TCACAGTCCA CTTCTTCCCT GGTGGACACA TCAGTCTCCA CCACTTCCCG GCCCCGCATG 
AAGAAGAAAC TGAAGATGCT TAACATTGCC AAGAAGATCT TACGTATCCA AAAGGAGACT 
CCGACCCTAC AACGGAAGGA GCCCCCACCT TCAGTGCTGG AGGCTGACCT CACAGAGGGA 
GACCTGGCCA ATTCCCACCT ACCCTCTGAG GTACTCTACA TGCTTAAGAA TGTTCGGGTG 
CTAGGTCACT TTGAGAAGCC GCTCTTCCTG GAGCTCTGCC GGCACATGGT CTTCCAGCGT 
CTCGGGCAGG GGGACTATGT TTTTCGGCCC GGTCAACCAG ATGCCAGCAT CTATGTGGTT 
CAAGATGGGC TGCTGGAGCT CTGCCTACCA GGGCCTGATG GGAAGGAGTG TGTGGTGAAG 
GAGGTGGTTC CTGGAGACAG CGTCAACAGC CTTCTGAGCA TCCTTGATGT CATCACGGGT 
CACCAGCATC CCCAGCGGAC TGTGTCAGCC AGGGCTGCAC GGGACTCCAC AGTGCTGAGA 
CTCCCAGTAG AAGCCTTCTC TGCTGTCTTC ACCAAGTACC CTGAGAGTTT GGTGCGAGTG 
GTACAGATCA TCATGGTAAG GCTGCAGAGA GTCACCTTCC TGGCGCTTCA CAATTACCTG 
GGTCTGACCA ATGAACTCTT CAGTCATGAG ATCCAGCCCC TACGCCTTTT CCCCAGCCCC 
GGCCTCCCGA CCCGAACCAG CCCTGTCCGT GGCTCTAAAC GGGTAGTCAG CACCTCAGGT 
ACTGAGGACA CCTCAAAGGA GACCTCTGGC CGGCCCCTGG ACTCCATTGG AGCTCCTCTG 
CCTGGACCTG CAGGGGATCC CGTGAAGCCC ACATCCTTAG AAGCACCCCC AGCCCCGTTA 
CTGAGCCGCT GCATCTCCAT GCCAGTAGAC ATCTCAGGCT TGCAAGGTGG CCCTCGTTCT 
GATTTTGACA TGGCCTATGA ACGTGGACGG ATATCTGTTT CTCTTCAAGA AGAGGCATCT 
GGGGGACCTC AGACAGCATC CCCTAGGACC CCCACTCAGG AGCTCCGGGA GCAGCCAGCG 
GGTGCCTGTG AATATAGCTA CTGTGAGGAT GAGTCAGCCA CAGGCGGATG TCCCTTTGGG 
CCCTACCAGG GCCGCCAGAC AAGCAGCATC TTTGAGGCTG CAAAGAGAGA GCTAGCCAAG 
CTGATGCGGA TTGAGGACCC CTCTCTACTG AACAGCAGAG TCTTGCTACA TCATGCCAAA 
GCCGGCACCA TCATAGCCCG CCAAGGGGAC CAGGATGTGA GCCTGCACTT TGTGCTCTGG 
GGCTGCCTGC ACGTGTACCA GCGCATGATT GACAAGGCTG AGGAAGTGTG CCTATTTGTG 
GCACAGCCAG GGGAGCTGGT GGGGCAACTG GCGGTGCTCA CTGGGGAACC TCTCATCTTC 
ACACTACGTG CCCAGAGAGA CTGCACCTTC CTGCGGATCT CCAAATCCCA CTTCTATGAG 
ATCATGCGTG CACAACCCAG TGTGGTACTG AGTGCAGCTC ACACGGTGGC TGCTAGAATG 
TCCCCCTTCG TGCGCCAGAT GGACTTTGCC ATTGACTGGA CAGCTGTGGA GGCTGGTCGT 
GCGCTCTACA GGCAGGGAGA CCGCTCCGAC TGCACCTACA TTGTACTCAA TGGACGTCTC 
CGTAGTGTCA TCCAACGAGG CAGTGGCAAG AAGGAGCTAG TTGGGGAGTA TGGTCGAGGG 
GATCTCATTG GCGTGGTGGA GGCACTGACC CGGCAGCCAC GAGCCACCAC TGTACACGCA 
GTGCGAGACA CTGAGCTGGC CAAGCTTCCC GAGGGCACCT TAGGCCACAT CAAACGTCGG 
TACCCACAGG TTGTGACCCG CCTTATTCAT CTGCTAAGCC AGAAAATTCT AGGCAATCTG 
CAGCAGTTGC AAGGACCCTT TCCAGGCTCC GGGCTCAGTG TTCCGCAGCA CTCGGAACTG 
ACCAACCCAG CCAGCAATCT GTCTACTGTA GCTATCCTCC CTGTGTGTGC TGAGGTGCCC 
ATGATGGCCT TTACTCTGGA ACTGCAGCAT GCTCTGCAAG CTATTGGTCC CACGCTCCTC 
CTTAACAGTG ACGTCATCCG GGCACTCCTG GGGGCTTCAG CACTGGATAG CATTCAAGAA 
TTCCGGCTGT CAGGGTGGTT GGCCCAGCAG GAAGATGCCC ATCGCATTGT GCTCTACCAA 
ACTGACACAT CCCTGACGCC TTGGACCGTC CGTTGTCTGC GCCAGGCCGA CTGCATCCTC 
ATTGTGGGCC TGGGGGACCA GGAACCCACT GTTGGCCAGC TGGAGCAAAT GTTAGAGAAC 
ACTGCTGTCC GTGCCTTGAA GCAACTGGTT TTGCTGCACC GGGAGGAAGG CCCTGGTCCC 
ACGCGCACTG TAGAGTGGCT CAACATGCGC AGCTGGTGCT CAGGGCACCT GCACCTGCGC 
TGTCCTCGCC GCCTCTTCTC ACGGCGCAGC CCAGCGAAAC TTCATGAGCT GTATGAGAAG 
GTTTTCTCCA GGCGTGCAGA CCGTCACAGC GACTTCTCCC GCTTGGCACG AGTACTCACA 
GGAAACACTA TTGCTCTGGT GCTGGGTGGG GGCGGAGCCA GAGGCTGCTC GCATATTGGG 
GTGCTGAAGG CATTAGAGGA GGCAGGAGTC CCAGTCGACC TTGTGGGAGG CACATCCATA 
GGTTCCTTCA TTGGGGCCTT GTATGCCGAG GAACGCAGCG CCAGCCGAAC TAAACAACGA 
GCCCGGGAGT GGGCCAAGAG CATGACTTCT GTACTGGAGC CTGTATTGGA CCTCACATAT 
CCTGTCACCT CCATGTTTAC TGGCTCAGCC TTTAACAGAA GTATCCACCG TGTCTTCCAG 
GATAAGCAGA TTGAGGACCT GTGGCTGCCT TACTTCAATG TGACCACAGA CATCACTGCC 
TCTGCCATGC GCGTCCACAA AGATGGCTCC CTGTGGCGGT ATGTACGTGC CAGCATGACG 
CTCTCGGGCT ACCTACCCCC GCTGTGCGAC CCAAAGGATG GGCACCTGCT CATGGATGGT 
GGCTACATCA ACAACCTGCC AGCGGATATT GCCCGAAGCA TGGGAGCCAA AACGGTCATT 
GCCATCGACG TTGGAAGCCA GGATGAGACA GATCTCAGCA CCTATGGGGA CAGCCTGTCT 
GGCTGGTGGT TGCTGTGGAA AAGGCTAAAC CCCTGGGCAG ACAAGGTAAA GGTTCCAGAC 
ATGGCTGAGA TCCAGTCCCG CTTGGCGTAC GTGTCCTGTG TGCGGCAGCT AGAAGTCGTC 
AAGTCCAGCT CCTATTGCGA GTACCTTCGT CCATCCATTG ACTGCTTCAA GACCATGGAC 
TTTGGAAAGT TTGACCAGAT CTATGATGTG GGTTACCAGT ATGGGAAGGC TGTCTTTGGA 
GGCTGGACCC GTGGTGAAGT CATTGAGAAA ATGCTCACAG ACCGGAGATC TACAGACCTT 
AATGAGAGTC GCCGTGCAGA TATACTTGCC TTCCCAAGTT CTGGGTTCAC TGACTTGGCT 
GAGATTGTGT CCCGGATCGA GCCACCAACA AGCTACGTCT CTGATGGCTG TGCTGATGGG 
GAGGAGTCGG ATTGCTTGAC AGAGTATGAG GAAGATGCAG GACCAGACTG CTCAAGAGAT 
GAAGGGGGCT CCCCTGAGGG TGCCAGCCCT AGCACTGCCT CAGAGGTGGA AGAAGAGAAG 
TCCACACTCC GGCAACGACG CTTTCTGCCT CAGGAGACTC CCAGCTCAGT CGCAGATGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_011242066.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240368.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X8, mRNA.

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CloneID	OMu69375
Clone ID Related Accession (Same CDS sequence)	XM_011242066.2 , XM_011242066.3
Accession Version	XM_011242066.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242066.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTGCC CAGCCTGTGC CGTTCGTTCC GCAGGTGCTG GGCGTGATGA TCGGGGCCGG 
AGTGGCGGTG CTGGTCACCG CCGTGCTCAT CCTCTTGGTG AAACTCCAGC CCCGGAGGGT 
CCCCGGTATC GGTTCCGGAA GCGGGACAAA GTGCTTTTCT ATGGCCGGAA GATTATGCGG 
AAGGTGTCAC AGTCCACTTC TTCCCTGGTG GACACATCAG TCTCCACCAC TTCCCGGCCC 
CGCATGAAGA AGAAACTGAA GATGCTTAAC ATTGCCAAGA AGATCTTACG TATCCAAAAG 
GAGACTCCGA CCCTACAACG GAAGGAGCCC CCACCTTCAG TGCTGGAGGC TGACCTCACA 
GAGGGAGACC TGGCCAATTC CCACCTACCC TCTGAGGTAC TCTACATGCT TAAGAATGTT 
CGGGTGCTAG GTCACTTTGA GAAGCCGCTC TTCCTGGAGC TCTGCCGGCA CATGGTCTTC 
CAGCGTCTCG GGCAGGGGGA CTATGTTTTT CGGCCCGGTC AACCAGATGC CAGCATCTAT 
GTGGTTCAAG ATGGGCTGCT GGAGCTCTGC CTACCAGGGC CTGATGGGAA GGAGTGTGTG 
GTGAAGGAGG TGGTTCCTGG AGACAGCGTC AACAGCCTTC TGAGCATCCT TGATGTCATC 
ACGGGTCACC AGCATCCCCA GCGGACTGTG TCAGCCAGGG CTGCACGGGA CTCCACAGTG 
CTGAGACTCC CAGTAGAAGC CTTCTCTGCT GTCTTCACCA AGTACCCTGA GAGTTTGGTG 
CGAGTGGTAC AGATCATCAT GGTAAGGCTG CAGAGAGTCA CCTTCCTGGC GCTTCACAAT 
TACCTGGGTC TGACCAATGA ACTCTTCAGT CATGAGATCC AGCCCCTACG CCTTTTCCCC 
AGCCCCGGCC TCCCGACCCG AACCAGCCCT GTCCGTGGCT CTAAACGGGT AGTCAGCACC 
TCAGGTACTG AGGACACCTC AAAGGAGACC TCTGGCCGGC CCCTGGACTC CATTGGAGCT 
CCTCTGCCTG GACCTGCAGG GGATCCCGTG AAGCCCACAT CCTTAGAAGC ACCCCCAGCC 
CCGTTACTGA GCCGCTGCAT CTCCATGCCA GTAGACATCT CAGGCTTGCA AGGTGGCCCT 
CGTTCTGATT TTGACATGGC CTATGAACGT GGACGGATAT CTGTTTCTCT TCAAGAAGAG 
GCATCTGGGG GACCTCAGAC AGCATCCCCT AGGACCCCCA CTCAGGAGCT CCGGGAGCAG 
CCAGCGGGTG CCTGTGAATA TAGCTACTGT GAGGATGAGT CAGCCACAGG CGGATGTCCC 
TTTGGGCCCT ACCAGGGCCG CCAGACAAGC AGCATCTTTG AGGCTGCAAA GAGAGAGCTA 
GCCAAGCTGA TGCGGATTGA GGACCCCTCT CTACTGAACA GCAGAGTCTT GCTACATCAT 
GCCAAAGCCG GCACCATCAT AGCCCGCCAA GGGGACCAGG ATGTGAGCCT GCACTTTGTG 
CTCTGGGGCT GCCTGCACGT GTACCAGCGC ATGATTGACA AGGCTGAGGA AGTGTGCCTA 
TTTGTGGCAC AGCCAGGGGA GCTGGTGGGG CAACTGGCGG TGCTCACTGG GGAACCTCTC 
ATCTTCACAC TACGTGCCCA GAGAGACTGC ACCTTCCTGC GGATCTCCAA ATCCCACTTC 
TATGAGATCA TGCGTGCACA ACCCAGTGTG GTACTGAGTG CAGCTCACAC GGTGGCTGCT 
AGAATGTCCC CCTTCGTGCG CCAGATGGAC TTTGCCATTG ACTGGACAGC TGTGGAGGCT 
GGTCGTGCGC TCTACAGGCA GGGAGACCGC TCCGACTGCA CCTACATTGT ACTCAATGGA 
CGTCTCCGTA GTGTCATCCA ACGAGGCAGT GGCAAGAAGG AGCTAGTTGG GGAGTATGGT 
CGAGGGGATC TCATTGGCGT GGTGGAGGCA CTGACCCGGC AGCCACGAGC CACCACTGTA 
CACGCAGTGC GAGACACTGA GCTGGCCAAG CTTCCCGAGG GCACCTTAGG CCACATCAAA 
CGTCGGTACC CACAGGTTGT GACCCGCCTT ATTCATCTGC TAAGCCAGAA AATTCTAGGC 
AATCTGCAGC AGTTGCAAGG ACCCTTTCCA GGCTCCGGGC TCAGTGTTCC GCAGCACTCG 
GAACTGACCA ACCCAGCCAG CAATCTGTCT ACTGTAGCTA TCCTCCCTGT GTGTGCTGAG 
GTGCCCATGA TGGCCTTTAC TCTGGAACTG CAGCATGCTC TGCAAGCTAT TGGTCCCACG 
CTCCTCCTTA ACAGTGACGT CATCCGGGCA CTCCTGGGGG CTTCAGCACT GGATAGCATT 
CAAGAATTCC GGCTGTCAGG GTGGTTGGCC CAGCAGGAAG ATGCCCATCG CATTGTGCTC 
TACCAAACTG ACACATCCCT GACGCCTTGG ACCGTCCGTT GTCTGCGCCA GGCCGACTGC 
ATCCTCATTG TGGGCCTGGG GGACCAGGAA CCCACTGTTG GCCAGCTGGA GCAAATGTTA 
GAGAACACTG CTGTCCGTGC CTTGAAGCAA CTGGTTTTGC TGCACCGGGA GGAAGGCCCT 
GGTCCCACGC GCACTGTAGA GTGGCTCAAC ATGCGCAGCT GGTGCTCAGG GCACCTGCAC 
CTGCGCTGTC CTCGCCGCCT CTTCTCACGG CGCAGCCCAG CGAAACTTCA TGAGCTGTAT 
GAGAAGGTTT TCTCCAGGCG TGCAGACCGT CACAGCGACT TCTCCCGCTT GGCACGAGTA 
CTCACAGGAA ACACTATTGC TCTGGTGCTG GGTGGGGGCG GAGCCAGAGG CTGCTCGCAT 
ATTGGGGTGC TGAAGGCATT AGAGGAGGCA GGAGTCCCAG TCGACCTTGT GGGAGGCACA 
TCCATAGGTT CCTTCATTGG GGCCTTGTAT GCCGAGGAAC GCAGCGCCAG CCGAACTAAA 
CAACGAGCCC GGGAGTGGGC CAAGAGCATG ACTTCTGTAC TGGAGCCTGT ATTGGACCTC 
ACATATCCTG TCACCTCCAT GTTTACTGGC TCAGCCTTTA ACAGAAGTAT CCACCGTGTC 
TTCCAGGATA AGCAGATTGA GGACCTGTGG CTGCCTTACT TCAATGTGAC CACAGACATC 
ACTGCCTCTG CCATGCGCGT CCACAAAGAT GGCTCCCTGT GGCGGTATGT ACGTGCCAGC 
ATGACGCTCT CGGGCTACCT ACCCCCGCTG TGCGACCCAA AGGATGGGCA CCTGCTCATG 
GATGGTGGCT ACATCAACAA CCTGCCAGCG GATATTGCCC GAAGCATGGG AGCCAAAACG 
GTCATTGCCA TCGACGTTGG AAGCCAGGAT GAGACAGATC TCAGCACCTA TGGGGACAGC 
CTGTCTGGCT GGTGGTTGCT GTGGAAAAGG CTAAACCCCT GGGCAGACAA GGTAAAGGTT 
CCAGACATGG CTGAGATCCA GTCCCGCTTG GCGTACGTGT CCTGTGTGCG GCAGCTAGAA 
GTCGTCAAGT CCAGCTCCTA TTGCGAGTAC CTTCGTCCAT CCATTGACTG CTTCAAGACC 
ATGGACTTTG GAAAGTTTGA CCAGATCTAT GATGTGGGTT ACCAGTATGG GAAGGCTGTC 
TTTGGAGGCT GGACCCGTGG TGAAGTCATT GAGAAAATGC TCACAGACCG GAGATCTACA 
GACCTTAATG AGAGTCGCCG TGCAGATATA CTTGCCTTCC CAAGTTCTGG GTTCACTGAC 
TTGGCTGAGA TTGTGTCCCG GATCGAGCCA CCAACAAGCT ACGTCTCTGA TGGCTGTGCT 
GATGGGGAGG AGTCGGATTG CTTGACAGAG TATGAGGAAG ATGCAGGACC AGACTGCTCA 
AGAGATGAAG GGGGCTCCCC TGAGGGTGCC AGCCCTAGCA CTGCCTCAGA GGTGGAAGAA 
GAGAAGTCCA CACTCCGGCA ACGACGCTTT CTGCCTCAGG AGACTCCCAG CTCAGTCGCA 
GATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240368.1
CDS	232..4200
Translation	MRCPACAVRSAGAGRDDRGRSGGAGHRRAHPLGETPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_011242066.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242066.3

ORF Insert Sequence:

ATGCGTTGCC CAGCCTGTGC CGTTCGTTCC GCAGGTGCTG GGCGTGATGA TCGGGGCCGG 
AGTGGCGGTG CTGGTCACCG CCGTGCTCAT CCTCTTGGTG AAACTCCAGC CCCGGAGGGT 
CCCCGGTATC GGTTCCGGAA GCGGGACAAA GTGCTTTTCT ATGGCCGGAA GATTATGCGG 
AAGGTGTCAC AGTCCACTTC TTCCCTGGTG GACACATCAG TCTCCACCAC TTCCCGGCCC 
CGCATGAAGA AGAAACTGAA GATGCTTAAC ATTGCCAAGA AGATCTTACG TATCCAAAAG 
GAGACTCCGA CCCTACAACG GAAGGAGCCC CCACCTTCAG TGCTGGAGGC TGACCTCACA 
GAGGGAGACC TGGCCAATTC CCACCTACCC TCTGAGGTAC TCTACATGCT TAAGAATGTT 
CGGGTGCTAG GTCACTTTGA GAAGCCGCTC TTCCTGGAGC TCTGCCGGCA CATGGTCTTC 
CAGCGTCTCG GGCAGGGGGA CTATGTTTTT CGGCCCGGTC AACCAGATGC CAGCATCTAT 
GTGGTTCAAG ATGGGCTGCT GGAGCTCTGC CTACCAGGGC CTGATGGGAA GGAGTGTGTG 
GTGAAGGAGG TGGTTCCTGG AGACAGCGTC AACAGCCTTC TGAGCATCCT TGATGTCATC 
ACGGGTCACC AGCATCCCCA GCGGACTGTG TCAGCCAGGG CTGCACGGGA CTCCACAGTG 
CTGAGACTCC CAGTAGAAGC CTTCTCTGCT GTCTTCACCA AGTACCCTGA GAGTTTGGTG 
CGAGTGGTAC AGATCATCAT GGTAAGGCTG CAGAGAGTCA CCTTCCTGGC GCTTCACAAT 
TACCTGGGTC TGACCAATGA ACTCTTCAGT CATGAGATCC AGCCCCTACG CCTTTTCCCC 
AGCCCCGGCC TCCCGACCCG AACCAGCCCT GTCCGTGGCT CTAAACGGGT AGTCAGCACC 
TCAGGTACTG AGGACACCTC AAAGGAGACC TCTGGCCGGC CCCTGGACTC CATTGGAGCT 
CCTCTGCCTG GACCTGCAGG GGATCCCGTG AAGCCCACAT CCTTAGAAGC ACCCCCAGCC 
CCGTTACTGA GCCGCTGCAT CTCCATGCCA GTAGACATCT CAGGCTTGCA AGGTGGCCCT 
CGTTCTGATT TTGACATGGC CTATGAACGT GGACGGATAT CTGTTTCTCT TCAAGAAGAG 
GCATCTGGGG GACCTCAGAC AGCATCCCCT AGGACCCCCA CTCAGGAGCT CCGGGAGCAG 
CCAGCGGGTG CCTGTGAATA TAGCTACTGT GAGGATGAGT CAGCCACAGG CGGATGTCCC 
TTTGGGCCCT ACCAGGGCCG CCAGACAAGC AGCATCTTTG AGGCTGCAAA GAGAGAGCTA 
GCCAAGCTGA TGCGGATTGA GGACCCCTCT CTACTGAACA GCAGAGTCTT GCTACATCAT 
GCCAAAGCCG GCACCATCAT AGCCCGCCAA GGGGACCAGG ATGTGAGCCT GCACTTTGTG 
CTCTGGGGCT GCCTGCACGT GTACCAGCGC ATGATTGACA AGGCTGAGGA AGTGTGCCTA 
TTTGTGGCAC AGCCAGGGGA GCTGGTGGGG CAACTGGCGG TGCTCACTGG GGAACCTCTC 
ATCTTCACAC TACGTGCCCA GAGAGACTGC ACCTTCCTGC GGATCTCCAA ATCCCACTTC 
TATGAGATCA TGCGTGCACA ACCCAGTGTG GTACTGAGTG CAGCTCACAC GGTGGCTGCT 
AGAATGTCCC CCTTCGTGCG CCAGATGGAC TTTGCCATTG ACTGGACAGC TGTGGAGGCT 
GGTCGTGCGC TCTACAGGCA GGGAGACCGC TCCGACTGCA CCTACATTGT ACTCAATGGA 
CGTCTCCGTA GTGTCATCCA ACGAGGCAGT GGCAAGAAGG AGCTAGTTGG GGAGTATGGT 
CGAGGGGATC TCATTGGCGT GGTGGAGGCA CTGACCCGGC AGCCACGAGC CACCACTGTA 
CACGCAGTGC GAGACACTGA GCTGGCCAAG CTTCCCGAGG GCACCTTAGG CCACATCAAA 
CGTCGGTACC CACAGGTTGT GACCCGCCTT ATTCATCTGC TAAGCCAGAA AATTCTAGGC 
AATCTGCAGC AGTTGCAAGG ACCCTTTCCA GGCTCCGGGC TCAGTGTTCC GCAGCACTCG 
GAACTGACCA ACCCAGCCAG CAATCTGTCT ACTGTAGCTA TCCTCCCTGT GTGTGCTGAG 
GTGCCCATGA TGGCCTTTAC TCTGGAACTG CAGCATGCTC TGCAAGCTAT TGGTCCCACG 
CTCCTCCTTA ACAGTGACGT CATCCGGGCA CTCCTGGGGG CTTCAGCACT GGATAGCATT 
CAAGAATTCC GGCTGTCAGG GTGGTTGGCC CAGCAGGAAG ATGCCCATCG CATTGTGCTC 
TACCAAACTG ACACATCCCT GACGCCTTGG ACCGTCCGTT GTCTGCGCCA GGCCGACTGC 
ATCCTCATTG TGGGCCTGGG GGACCAGGAA CCCACTGTTG GCCAGCTGGA GCAAATGTTA 
GAGAACACTG CTGTCCGTGC CTTGAAGCAA CTGGTTTTGC TGCACCGGGA GGAAGGCCCT 
GGTCCCACGC GCACTGTAGA GTGGCTCAAC ATGCGCAGCT GGTGCTCAGG GCACCTGCAC 
CTGCGCTGTC CTCGCCGCCT CTTCTCACGG CGCAGCCCAG CGAAACTTCA TGAGCTGTAT 
GAGAAGGTTT TCTCCAGGCG TGCAGACCGT CACAGCGACT TCTCCCGCTT GGCACGAGTA 
CTCACAGGAA ACACTATTGC TCTGGTGCTG GGTGGGGGCG GAGCCAGAGG CTGCTCGCAT 
ATTGGGGTGC TGAAGGCATT AGAGGAGGCA GGAGTCCCAG TCGACCTTGT GGGAGGCACA 
TCCATAGGTT CCTTCATTGG GGCCTTGTAT GCCGAGGAAC GCAGCGCCAG CCGAACTAAA 
CAACGAGCCC GGGAGTGGGC CAAGAGCATG ACTTCTGTAC TGGAGCCTGT ATTGGACCTC 
ACATATCCTG TCACCTCCAT GTTTACTGGC TCAGCCTTTA ACAGAAGTAT CCACCGTGTC 
TTCCAGGATA AGCAGATTGA GGACCTGTGG CTGCCTTACT TCAATGTGAC CACAGACATC 
ACTGCCTCTG CCATGCGCGT CCACAAAGAT GGCTCCCTGT GGCGGTATGT ACGTGCCAGC 
ATGACGCTCT CGGGCTACCT ACCCCCGCTG TGCGACCCAA AGGATGGGCA CCTGCTCATG 
GATGGTGGCT ACATCAACAA CCTGCCAGCG GATATTGCCC GAAGCATGGG AGCCAAAACG 
GTCATTGCCA TCGACGTTGG AAGCCAGGAT GAGACAGATC TCAGCACCTA TGGGGACAGC 
CTGTCTGGCT GGTGGTTGCT GTGGAAAAGG CTAAACCCCT GGGCAGACAA GGTAAAGGTT 
CCAGACATGG CTGAGATCCA GTCCCGCTTG GCGTACGTGT CCTGTGTGCG GCAGCTAGAA 
GTCGTCAAGT CCAGCTCCTA TTGCGAGTAC CTTCGTCCAT CCATTGACTG CTTCAAGACC 
ATGGACTTTG GAAAGTTTGA CCAGATCTAT GATGTGGGTT ACCAGTATGG GAAGGCTGTC 
TTTGGAGGCT GGACCCGTGG TGAAGTCATT GAGAAAATGC TCACAGACCG GAGATCTACA 
GACCTTAATG AGAGTCGCCG TGCAGATATA CTTGCCTTCC CAAGTTCTGG GTTCACTGAC 
TTGGCTGAGA TTGTGTCCCG GATCGAGCCA CCAACAAGCT ACGTCTCTGA TGGCTGTGCT 
GATGGGGAGG AGTCGGATTG CTTGACAGAG TATGAGGAAG ATGCAGGACC AGACTGCTCA 
AGAGATGAAG GGGGCTCCCC TGAGGGTGCC AGCCCTAGCA CTGCCTCAGA GGTGGAAGAA 
GAGAAGTCCA CACTCCGGCA ACGACGCTTT CTGCCTCAGG AGACTCCCAG CTCAGTCGCA 
GATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_017312891.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168380.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X13, mRNA.

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CloneID	OMu82536
Clone ID Related Accession (Same CDS sequence)	XM_017312891.1
Accession Version	XM_017312891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	neuropathy target esterase isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTGCC CAGCCTGTGC CGTTCGTTCC GCAGGTGCTG GGCGTGATGA TCGGGGCCGG 
AGTGGCGGTG CTGGTCACCG CCGTGCTCAT CCTCTTGGTG AAACTCCAGC CCCGGAGGGT 
CCCCGGTATC GGTTCCGGAA GCGGGACAAA GTGCTTTTCT ATGGCCGGAA GATTATGCGG 
AAGGTGTCAC AGTCCACTTC TTCCCTGGTG GACACATCAG TCTCCACCAC TTCCCGGCCC 
CGCATGAAGA AGAAACTGAA GATGCTTAAC ATTGCCAAGA AGATCTTACG TATCCAAAAG 
GAGACTCCGA CCCTACAACG GAAGGAGCCC CCACCTTCAG TGCTGGAGGC TGACCTCACA 
GAGGGAGACC TGGCCAATTC CCACCTACCC TCTGAGGTAC TCTACATGCT TAAGAATGTT 
CGGGTGCTAG GTCACTTTGA GAAGCCGCTC TTCCTGGAGC TCTGCCGGCA CATGGTCTTC 
CAGCGTCTCG GGCAGGGGGA CTATGTTTTT CGGCCCGGTC AACCAGATGC CAGCATCTAT 
GTGGTTCAAG ATGGGCTGCT GGAGCTCTGC CTACCAGGGC CTGATGGGAA GGAGTGTGTG 
GTGAAGGAGG TGGTTCCTGG AGACAGCGTC AACAGCCTTC TGAGCATCCT TGATGTCATC 
ACGGGTCACC AGCATCCCCA GCGGACTGTG TCAGCCAGGG CTGCACGGGA CTCCACAGTG 
CTGAGACTCC CAGTAGAAGC CTTCTCTGCT GTCTTCACCA AGTACCCTGA GAGTTTGGTG 
CGAGTGGTAC AGATCATCAT GGTAAGGCTG CAGAGAGTCA CCTTCCTGGC GCTTCACAAT 
TACCTGGGTC TGACCAATGA ACTCTTCAGT CATGAGATCC AGCCCCTACG CCTTTTCCCC 
AGCCCCGGCC TCCCGACCCG AACCAGCCCT GTCCGTGGCT CTAAACGGGT AGTCAGCACC 
TCAGGTACTG AGGACACCTC AAAGGAGACC TCTGGCCGGC CCCTGGACTC CATTGGAGCT 
CCTCTGCCTG GACCTGCAGG GGATCCCGTG AAGCCCACAT CCTTAGAAGC ACCCCCAGCC 
CCGTTACTGA GCCGCTGCAT CTCCATGCCA GTAGACATCT CAGGCTTGCA AGGTGGCCCT 
CGTTCTGATT TTGACATGGC CTATGAACGT GGACGGATAT CTGTTTCTCT TCAAGAAGAG 
GCATCTGGGG GACCTCAGAC AGCATCCCCT AGGGAGCTCC GGGAGCAGCC AGCGGGTGCC 
TGTGAATATA GCTACTGTGA GGATGAGTCA GCCACAGGCG GATGTCCCTT TGGGCCCTAC 
CAGGGCCGCC AGACAAGCAG CATCTTTGAG GCTGCAAAGA GAGAGCTAGC CAAGCTGATG 
CGGATTGAGG ACCCCTCTCT ACTGAACAGC AGAGTCTTGC TACATCATGC CAAAGCCGGC 
ACCATCATAG CCCGCCAAGG GGACCAGGAT GTGAGCCTGC ACTTTGTGCT CTGGGGCTGC 
CTGCACGTGT ACCAGCGCAT GATTGACAAG GCTGAGGAAG TGTGCCTATT TGTGGCACAG 
CCAGGGGAGC TGGTGGGGCA ACTGGCGGTG CTCACTGGGG AACCTCTCAT CTTCACACTA 
CGTGCCCAGA GAGACTGCAC CTTCCTGCGG ATCTCCAAAT CCCACTTCTA TGAGATCATG 
CGTGCACAAC CCAGTGTGGT ACTGAGTGCA GCTCACACGG TGGCTGCTAG AATGTCCCCC 
TTCGTGCGCC AGATGGACTT TGCCATTGAC TGGACAGCTG TGGAGGCTGG TCGTGCGCTC 
TACAGGCAGG GAGACCGCTC CGACTGCACC TACATTGTAC TCAATGGACG TCTCCGTAGT 
GTCATCCAAC GAGGCAGTGG CAAGAAGGAG CTAGTTGGGG AGTATGGTCG AGGGGATCTC 
ATTGGCGTGG TGGAGGCACT GACCCGGCAG CCACGAGCCA CCACTGTACA CGCAGTGCGA 
GACACTGAGC TGGCCAAGCT TCCCGAGGGC ACCTTAGGCC ACATCAAACG TCGGTACCCA 
CAGGTTGTGA CCCGCCTTAT TCATCTGCTA AGCCAGAAAA TTCTAGGCAA TCTGCAGCAG 
TTGCAAGGAC CCTTTCCAGG CTCCGGGCTC AGTGTTCCGC AGCACTCGGA ACTGACCAAC 
CCAGCCAGCA ATCTGTCTAC TGTAGCTATC CTCCCTGTGT GTGCTGAGGT GCCCATGATG 
GCCTTTACTC TGGAACTGCA GCATGCTCTG CAAGCTATTG GTCCCACGCT CCTCCTTAAC 
AGTGACGTCA TCCGGGCACT CCTGGGGGCT TCAGCACTGG ATAGCATTCA AGAATTCCGG 
CTGTCAGGGT GGTTGGCCCA GCAGGAAGAT GCCCATCGCA TTGTGCTCTA CCAAACTGAC 
ACATCCCTGA CGCCTTGGAC CGTCCGTTGT CTGCGCCAGG CCGACTGCAT CCTCATTGTG 
GGCCTGGGGG ACCAGGAACC CACTGTTGGC CAGCTGGAGC AAATGTTAGA GAACACTGCT 
GTCCGTGCCT TGAAGCAACT GGTTTTGCTG CACCGGGAGG AAGGCCCTGG TCCCACGCGC 
ACTGTAGAGT GGCTCAACAT GCGCAGCTGG TGCTCAGGGC ACCTGCACCT GCGCTGTCCT 
CGCCGCCTCT TCTCACGGCG CAGCCCAGCG AAACTTCATG AGCTGTATGA GAAGGTTTTC 
TCCAGGCGTG CAGACCGTCA CAGCGACTTC TCCCGCTTGG CACGAGTACT CACAGGAAAC 
ACTATTGCTC TGGTGCTGGG TGGGGGCGGA GCCAGAGGCT GCTCGCATAT TGGGGTGCTG 
AAGGCATTAG AGGAGGCAGG AGTCCCAGTC GACCTTGTGG GAGGCACATC CATAGGTTCC 
TTCATTGGGG CCTTGTATGC CGAGGAACGC AGCGCCAGCC GAACTAAACA ACGAGCCCGG 
GAGTGGGCCA AGAGCATGAC TTCTGTACTG GAGCCTGTAT TGGACCTCAC ATATCCTGTC 
ACCTCCATGT TTACTGGCTC AGCCTTTAAC AGAAGTATCC ACCGTGTCTT CCAGGATAAG 
CAGATTGAGG ACCTGTGGCT GCCTTACTTC AATGTGACCA CAGACATCAC TGCCTCTGCC 
ATGCGCGTCC ACAAAGATGG CTCCCTGTGG CGGTATGTAC GTGCCAGCAT GACGCTCTCG 
GGCTACCTAC CCCCGCTGTG CGACCCAAAG GATGGGCACC TGCTCATGGA TGGTGGCTAC 
ATCAACAACC TGCCAGCGGA TATTGCCCGA AGCATGGGAG CCAAAACGGT CATTGCCATC 
GACGTTGGAA GCCAGGATGA GACAGATCTC AGCACCTATG GGGACAGCCT GTCTGGCTGG 
TGGTTGCTGT GGAAAAGGCT AAACCCCTGG GCAGACAAGG TAAAGGTTCC AGACATGGCT 
GAGATCCAGT CCCGCTTGGC GTACGTGTCC TGTGTGCGGC AGCTAGAAGT CGTCAAGTCC 
AGCTCCTATT GCGAGTACCT TCGTCCATCC ATTGACTGCT TCAAGACCAT GGACTTTGGA 
AAGTTTGACC AGATCTATGA TGTGGGTTAC CAGTATGGGA AGGCTGTCTT TGGAGGCTGG 
ACCCGTGGTG AAGTCATTGA GAAAATGCTC ACAGACCGGA GATCTACAGA CCTTAATGAG 
AGTCGCCGTG CAGATATACT TGCCTTCCCA AGTTCTGGGT TCACTGACTT GGCTGAGATT 
GTGTCCCGGA TCGAGCCACC AACAAGCTAC GTCTCTGATG GCTGTGCTGA TGGGGAGGAG 
TCGGATTGCT TGACAGAGTA TGAGGAAGAT GCAGGACCAG ACTGCTCAAG AGATGAAGGG 
GGCTCCCCTG AGGGTGCCAG CCCTAGCACT GCCTCAGAGG TGGAAGAAGA GAAGTCCACA 
CTCCGGCAAC GACGCTTTCT GCCTCAGGAG ACTCCCAGCT CAGTCGCAGA TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168380.1
CDS	237..4193
Translation	MRCPACAVRSAGAGRDDRGRSGGAGHRRAHPLGETPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_017312891.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312891.1

ORF Insert Sequence:

ATGCGTTGCC CAGCCTGTGC CGTTCGTTCC GCAGGTGCTG GGCGTGATGA TCGGGGCCGG 
AGTGGCGGTG CTGGTCACCG CCGTGCTCAT CCTCTTGGTG AAACTCCAGC CCCGGAGGGT 
CCCCGGTATC GGTTCCGGAA GCGGGACAAA GTGCTTTTCT ATGGCCGGAA GATTATGCGG 
AAGGTGTCAC AGTCCACTTC TTCCCTGGTG GACACATCAG TCTCCACCAC TTCCCGGCCC 
CGCATGAAGA AGAAACTGAA GATGCTTAAC ATTGCCAAGA AGATCTTACG TATCCAAAAG 
GAGACTCCGA CCCTACAACG GAAGGAGCCC CCACCTTCAG TGCTGGAGGC TGACCTCACA 
GAGGGAGACC TGGCCAATTC CCACCTACCC TCTGAGGTAC TCTACATGCT TAAGAATGTT 
CGGGTGCTAG GTCACTTTGA GAAGCCGCTC TTCCTGGAGC TCTGCCGGCA CATGGTCTTC 
CAGCGTCTCG GGCAGGGGGA CTATGTTTTT CGGCCCGGTC AACCAGATGC CAGCATCTAT 
GTGGTTCAAG ATGGGCTGCT GGAGCTCTGC CTACCAGGGC CTGATGGGAA GGAGTGTGTG 
GTGAAGGAGG TGGTTCCTGG AGACAGCGTC AACAGCCTTC TGAGCATCCT TGATGTCATC 
ACGGGTCACC AGCATCCCCA GCGGACTGTG TCAGCCAGGG CTGCACGGGA CTCCACAGTG 
CTGAGACTCC CAGTAGAAGC CTTCTCTGCT GTCTTCACCA AGTACCCTGA GAGTTTGGTG 
CGAGTGGTAC AGATCATCAT GGTAAGGCTG CAGAGAGTCA CCTTCCTGGC GCTTCACAAT 
TACCTGGGTC TGACCAATGA ACTCTTCAGT CATGAGATCC AGCCCCTACG CCTTTTCCCC 
AGCCCCGGCC TCCCGACCCG AACCAGCCCT GTCCGTGGCT CTAAACGGGT AGTCAGCACC 
TCAGGTACTG AGGACACCTC AAAGGAGACC TCTGGCCGGC CCCTGGACTC CATTGGAGCT 
CCTCTGCCTG GACCTGCAGG GGATCCCGTG AAGCCCACAT CCTTAGAAGC ACCCCCAGCC 
CCGTTACTGA GCCGCTGCAT CTCCATGCCA GTAGACATCT CAGGCTTGCA AGGTGGCCCT 
CGTTCTGATT TTGACATGGC CTATGAACGT GGACGGATAT CTGTTTCTCT TCAAGAAGAG 
GCATCTGGGG GACCTCAGAC AGCATCCCCT AGGGAGCTCC GGGAGCAGCC AGCGGGTGCC 
TGTGAATATA GCTACTGTGA GGATGAGTCA GCCACAGGCG GATGTCCCTT TGGGCCCTAC 
CAGGGCCGCC AGACAAGCAG CATCTTTGAG GCTGCAAAGA GAGAGCTAGC CAAGCTGATG 
CGGATTGAGG ACCCCTCTCT ACTGAACAGC AGAGTCTTGC TACATCATGC CAAAGCCGGC 
ACCATCATAG CCCGCCAAGG GGACCAGGAT GTGAGCCTGC ACTTTGTGCT CTGGGGCTGC 
CTGCACGTGT ACCAGCGCAT GATTGACAAG GCTGAGGAAG TGTGCCTATT TGTGGCACAG 
CCAGGGGAGC TGGTGGGGCA ACTGGCGGTG CTCACTGGGG AACCTCTCAT CTTCACACTA 
CGTGCCCAGA GAGACTGCAC CTTCCTGCGG ATCTCCAAAT CCCACTTCTA TGAGATCATG 
CGTGCACAAC CCAGTGTGGT ACTGAGTGCA GCTCACACGG TGGCTGCTAG AATGTCCCCC 
TTCGTGCGCC AGATGGACTT TGCCATTGAC TGGACAGCTG TGGAGGCTGG TCGTGCGCTC 
TACAGGCAGG GAGACCGCTC CGACTGCACC TACATTGTAC TCAATGGACG TCTCCGTAGT 
GTCATCCAAC GAGGCAGTGG CAAGAAGGAG CTAGTTGGGG AGTATGGTCG AGGGGATCTC 
ATTGGCGTGG TGGAGGCACT GACCCGGCAG CCACGAGCCA CCACTGTACA CGCAGTGCGA 
GACACTGAGC TGGCCAAGCT TCCCGAGGGC ACCTTAGGCC ACATCAAACG TCGGTACCCA 
CAGGTTGTGA CCCGCCTTAT TCATCTGCTA AGCCAGAAAA TTCTAGGCAA TCTGCAGCAG 
TTGCAAGGAC CCTTTCCAGG CTCCGGGCTC AGTGTTCCGC AGCACTCGGA ACTGACCAAC 
CCAGCCAGCA ATCTGTCTAC TGTAGCTATC CTCCCTGTGT GTGCTGAGGT GCCCATGATG 
GCCTTTACTC TGGAACTGCA GCATGCTCTG CAAGCTATTG GTCCCACGCT CCTCCTTAAC 
AGTGACGTCA TCCGGGCACT CCTGGGGGCT TCAGCACTGG ATAGCATTCA AGAATTCCGG 
CTGTCAGGGT GGTTGGCCCA GCAGGAAGAT GCCCATCGCA TTGTGCTCTA CCAAACTGAC 
ACATCCCTGA CGCCTTGGAC CGTCCGTTGT CTGCGCCAGG CCGACTGCAT CCTCATTGTG 
GGCCTGGGGG ACCAGGAACC CACTGTTGGC CAGCTGGAGC AAATGTTAGA GAACACTGCT 
GTCCGTGCCT TGAAGCAACT GGTTTTGCTG CACCGGGAGG AAGGCCCTGG TCCCACGCGC 
ACTGTAGAGT GGCTCAACAT GCGCAGCTGG TGCTCAGGGC ACCTGCACCT GCGCTGTCCT 
CGCCGCCTCT TCTCACGGCG CAGCCCAGCG AAACTTCATG AGCTGTATGA GAAGGTTTTC 
TCCAGGCGTG CAGACCGTCA CAGCGACTTC TCCCGCTTGG CACGAGTACT CACAGGAAAC 
ACTATTGCTC TGGTGCTGGG TGGGGGCGGA GCCAGAGGCT GCTCGCATAT TGGGGTGCTG 
AAGGCATTAG AGGAGGCAGG AGTCCCAGTC GACCTTGTGG GAGGCACATC CATAGGTTCC 
TTCATTGGGG CCTTGTATGC CGAGGAACGC AGCGCCAGCC GAACTAAACA ACGAGCCCGG 
GAGTGGGCCA AGAGCATGAC TTCTGTACTG GAGCCTGTAT TGGACCTCAC ATATCCTGTC 
ACCTCCATGT TTACTGGCTC AGCCTTTAAC AGAAGTATCC ACCGTGTCTT CCAGGATAAG 
CAGATTGAGG ACCTGTGGCT GCCTTACTTC AATGTGACCA CAGACATCAC TGCCTCTGCC 
ATGCGCGTCC ACAAAGATGG CTCCCTGTGG CGGTATGTAC GTGCCAGCAT GACGCTCTCG 
GGCTACCTAC CCCCGCTGTG CGACCCAAAG GATGGGCACC TGCTCATGGA TGGTGGCTAC 
ATCAACAACC TGCCAGCGGA TATTGCCCGA AGCATGGGAG CCAAAACGGT CATTGCCATC 
GACGTTGGAA GCCAGGATGA GACAGATCTC AGCACCTATG GGGACAGCCT GTCTGGCTGG 
TGGTTGCTGT GGAAAAGGCT AAACCCCTGG GCAGACAAGG TAAAGGTTCC AGACATGGCT 
GAGATCCAGT CCCGCTTGGC GTACGTGTCC TGTGTGCGGC AGCTAGAAGT CGTCAAGTCC 
AGCTCCTATT GCGAGTACCT TCGTCCATCC ATTGACTGCT TCAAGACCAT GGACTTTGGA 
AAGTTTGACC AGATCTATGA TGTGGGTTAC CAGTATGGGA AGGCTGTCTT TGGAGGCTGG 
ACCCGTGGTG AAGTCATTGA GAAAATGCTC ACAGACCGGA GATCTACAGA CCTTAATGAG 
AGTCGCCGTG CAGATATACT TGCCTTCCCA AGTTCTGGGT TCACTGACTT GGCTGAGATT 
GTGTCCCGGA TCGAGCCACC AACAAGCTAC GTCTCTGATG GCTGTGCTGA TGGGGAGGAG 
TCGGATTGCT TGACAGAGTA TGAGGAAGAT GCAGGACCAG ACTGCTCAAG AGATGAAGGG 
GGCTCCCCTG AGGGTGCCAG CCCTAGCACT GCCTCAGAGG TGGAAGAAGA GAAGTCCACA 
CTCCGGCAAC GACGCTTTCT GCCTCAGGAG ACTCCCAGCT CAGTCGCAGA TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( NM_015801.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_056616.2 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 2, mRNA.

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CloneID	OMu10290
Clone ID Related Accession (Same CDS sequence)	XM_006508825.3 , NM_015801.2
Accession Version	NM_015801.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY322839.1, BC056999.1 and AV582056.1. On Mar 25, 2008 this sequence version replaced NM_015801.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF173829.1, SRR1660813.270623.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCC CACTGCAAAC CGGAATGGTG CTGGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGCTGGTCA CCGCCGTGCT CATCCTCTTG GTGGTGCGAA GGCTGCGGGT GCAGAAAACT 
CCAGCCCCGG AGGGTCCCCG GTATCGGTTC CGGAAGCGGG ACAAAGTGCT TTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAGTCC ACTTCTTCCC TGGTGGACAC ATCAGTCTCC 
ACCACTTCCC GGCCCCGCAT GAAGAAGAAA CTGAAGATGC TTAACATTGC CAAGAAGATC 
TTACGTATCC AAAAGGAGAC TCCGACCCTA CAACGGAAGG AGCCCCCACC TTCAGTGCTG 
GAGGCTGACC TCACAGAGGG AGACCTGGCC AATTCCCACC TACCCTCTGA GGTACTCTAC 
ATGCTTAAGA ATGTTCGGGT GCTAGGTCAC TTTGAGAAGC CGCTCTTCCT GGAGCTCTGC 
CGGCACATGG TCTTCCAGCG TCTCGGGCAG GGGGACTATG TTTTTCGGCC CGGTCAACCA 
GATGCCAGCA TCTATGTGGT TCAAGATGGG CTGCTGGAGC TCTGCCTACC AGGGCCTGAT 
GGGAAGGAGT GTGTGGTGAA GGAGGTGGTT CCTGGAGACA GCGTCAACAG CCTTCTGAGC 
ATCCTTGATG TCATCACGGG TCACCAGCAT CCCCAGCGGA CTGTGTCAGC CAGGGCTGCA 
CGGGACTCCA CAGTGCTGAG ACTCCCAGTA GAAGCCTTCT CTGCTGTCTT CACCAAGTAC 
CCTGAGAGTT TGGTGCGAGT GGTACAGATC ATCATGGTAA GGCTGCAGAG AGTCACCTTC 
CTGGCGCTTC ACAATTACCT GGGTCTGACC AATGAACTCT TCAGTCATGA GATCCAGCCC 
CTACGCCTTT TCCCCAGCCC CGGCCTCCCG ACCCGAACCA GCCCTGTCCG TGGCTCTAAA 
CGGGTAGTCA GCACCTCAGG TACTGAGGAC ACCTCAAAGG AGACCTCTGG CCGGCCCCTG 
GACTCCATTG GAGCTCCTCT GCCTGGACCT GCAGGGGATC CCGTGAAGCC CACATCCTTA 
GAAGCACCCC CAGCCCCGTT ACTGAGCCGC TGCATCTCCA TGCCAGTAGA CATCTCAGGC 
TTGCAAGGTG GCCCTCGTTC TGATTTTGAC ATGGCCTATG AACGTGGACG GATATCTGTT 
TCTCTTCAAG AAGAGGCATC TGGGGGACCT CAGACAGCAT CCCCTAGGAC CCCCACTCAG 
GAGCTCCGGG AGCAGCCAGC GGGTGCCTGT GAATATAGCT ACTGTGAGGA TGAGTCAGCC 
ACAGGCGGAT GTCCCTTTGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCT 
GCAAAGAGAG AGCTAGCCAA GCTGATGCGG ATTGAGGACC CCTCTCTACT GAACAGCAGA 
GTCTTGCTAC ATCATGCCAA AGCCGGCACC ATCATAGCCC GCCAAGGGGA CCAGGATGTG 
AGCCTGCACT TTGTGCTCTG GGGCTGCCTG CACGTGTACC AGCGCATGAT TGACAAGGCT 
GAGGAAGTGT GCCTATTTGT GGCACAGCCA GGGGAGCTGG TGGGGCAACT GGCGGTGCTC 
ACTGGGGAAC CTCTCATCTT CACACTACGT GCCCAGAGAG ACTGCACCTT CCTGCGGATC 
TCCAAATCCC ACTTCTATGA GATCATGCGT GCACAACCCA GTGTGGTACT GAGTGCAGCT 
CACACGGTGG CTGCTAGAAT GTCCCCCTTC GTGCGCCAGA TGGACTTTGC CATTGACTGG 
ACAGCTGTGG AGGCTGGTCG TGCGCTCTAC AGGCAGGGAG ACCGCTCCGA CTGCACCTAC 
ATTGTACTCA ATGGACGTCT CCGTAGTGTC ATCCAACGAG GCAGTGGCAA GAAGGAGCTA 
GTTGGGGAGT ATGGTCGAGG GGATCTCATT GGCGTGGTGG AGGCACTGAC CCGGCAGCCA 
CGAGCCACCA CTGTACACGC AGTGCGAGAC ACTGAGCTGG CCAAGCTTCC CGAGGGCACC 
TTAGGCCACA TCAAACGTCG GTACCCACAG GTTGTGACCC GCCTTATTCA TCTGCTAAGC 
CAGAAAATTC TAGGCAATCT GCAGCAGTTG CAAGGACCCT TTCCAGGCTC CGGGCTCAGT 
GTTCCGCAGC ACTCGGAACT GACCAACCCA GCCAGCAATC TGTCTACTGT AGCTATCCTC 
CCTGTGTGTG CTGAGGTGCC CATGATGGCC TTTACTCTGG AACTGCAGCA TGCTCTGCAA 
GCTATTGGTC CCACGCTCCT CCTTAACAGT GACGTCATCC GGGCACTCCT GGGGGCTTCA 
GCACTGGATA GCATTCAAGA ATTCCGGCTG TCAGGGTGGT TGGCCCAGCA GGAAGATGCC 
CATCGCATTG TGCTCTACCA AACTGACACA TCCCTGACGC CTTGGACCGT CCGTTGTCTG 
CGCCAGGCCG ACTGCATCCT CATTGTGGGC CTGGGGGACC AGGAACCCAC TGTTGGCCAG 
CTGGAGCAAA TGTTAGAGAA CACTGCTGTC CGTGCCTTGA AGCAACTGGT TTTGCTGCAC 
CGGGAGGAAG GCCCTGGTCC CACGCGCACT GTAGAGTGGC TCAACATGCG CAGCTGGTGC 
TCAGGGCACC TGCACCTGCG CTGTCCTCGC CGCCTCTTCT CACGGCGCAG CCCAGCGAAA 
CTTCATGAGC TGTATGAGAA GGTTTTCTCC AGGCGTGCAG ACCGTCACAG CGACTTCTCC 
CGCTTGGCAC GAGTACTCAC AGGAAACACT ATTGCTCTGG TGCTGGGTGG GGGCGGAGCC 
AGAGGCTGCT CGCATATTGG GGTGCTGAAG GCATTAGAGG AGGCAGGAGT CCCAGTCGAC 
CTTGTGGGAG GCACATCCAT AGGTTCCTTC ATTGGGGCCT TGTATGCCGA GGAACGCAGC 
GCCAGCCGAA CTAAACAACG AGCCCGGGAG TGGGCCAAGA GCATGACTTC TGTACTGGAG 
CCTGTATTGG ACCTCACATA TCCTGTCACC TCCATGTTTA CTGGCTCAGC CTTTAACAGA 
AGTATCCACC GTGTCTTCCA GGATAAGCAG ATTGAGGACC TGTGGCTGCC TTACTTCAAT 
GTGACCACAG ACATCACTGC CTCTGCCATG CGCGTCCACA AAGATGGCTC CCTGTGGCGG 
TATGTACGTG CCAGCATGAC GCTCTCGGGC TACCTACCCC CGCTGTGCGA CCCAAAGGAT 
GGGCACCTGC TCATGGATGG TGGCTACATC AACAACCTGC CAGCGGATAT TGCCCGAAGC 
ATGGGAGCCA AAACGGTCAT TGCCATCGAC GTTGGAAGCC AGGATGAGAC AGATCTCAGC 
ACCTATGGGG ACAGCCTGTC TGGCTGGTGG TTGCTGTGGA AAAGGCTAAA CCCCTGGGCA 
GACAAGGTAA AGGTTCCAGA CATGGCTGAG ATCCAGTCCC GCTTGGCGTA CGTGTCCTGT 
GTGCGGCAGC TAGAAGTCGT CAAGTCCAGC TCCTATTGCG AGTACCTTCG TCCATCCATT 
GACTGCTTCA AGACCATGGA CTTTGGAAAG TTTGACCAGA TCTATGATGT GGGTTACCAG 
TATGGGAAGG CTGTCTTTGG AGGCTGGACC CGTGGTGAAG TCATTGAGAA AATGCTCACA 
GACCGGAGAT CTACAGACCT TAATGAGAGT CGCCGTGCAG ATATACTTGC CTTCCCAAGT 
TCTGGGTTCA CTGACTTGGC TGAGATTGTG TCCCGGATCG AGCCACCAAC AAGCTACGTC 
TCTGATGGCT GTGCTGATGG GGAGGAGTCG GATTGCTTGA CAGAGTATGA GGAAGATGCA 
GGACCAGACT GCTCAAGAGA TGAAGGGGGC TCCCCTGAGG GTGCCAGCCC TAGCACTGCC 
TCAGAGGTGG AAGAAGAGAA GTCCACACTC CGGCAACGAC GCTTTCTGCC TCAGGAGACT 
CCCAGCTCAG TCGCAGATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056616.2
CDS	216..4199
Translation	MEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	NM_015801.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015801.2

ORF Insert Sequence:

ATGGAGGCCC CACTGCAAAC CGGAATGGTG CTGGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGCTGGTCA CCGCCGTGCT CATCCTCTTG GTGGTGCGAA GGCTGCGGGT GCAGAAAACT 
CCAGCCCCGG AGGGTCCCCG GTATCGGTTC CGGAAGCGGG ACAAAGTGCT TTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAGTCC ACTTCTTCCC TGGTGGACAC ATCAGTCTCC 
ACCACTTCCC GGCCCCGCAT GAAGAAGAAA CTGAAGATGC TTAACATTGC CAAGAAGATC 
TTACGTATCC AAAAGGAGAC TCCGACCCTA CAACGGAAGG AGCCCCCACC TTCAGTGCTG 
GAGGCTGACC TCACAGAGGG AGACCTGGCC AATTCCCACC TACCCTCTGA GGTACTCTAC 
ATGCTTAAGA ATGTTCGGGT GCTAGGTCAC TTTGAGAAGC CGCTCTTCCT GGAGCTCTGC 
CGGCACATGG TCTTCCAGCG TCTCGGGCAG GGGGACTATG TTTTTCGGCC CGGTCAACCA 
GATGCCAGCA TCTATGTGGT TCAAGATGGG CTGCTGGAGC TCTGCCTACC AGGGCCTGAT 
GGGAAGGAGT GTGTGGTGAA GGAGGTGGTT CCTGGAGACA GCGTCAACAG CCTTCTGAGC 
ATCCTTGATG TCATCACGGG TCACCAGCAT CCCCAGCGGA CTGTGTCAGC CAGGGCTGCA 
CGGGACTCCA CAGTGCTGAG ACTCCCAGTA GAAGCCTTCT CTGCTGTCTT CACCAAGTAC 
CCTGAGAGTT TGGTGCGAGT GGTACAGATC ATCATGGTAA GGCTGCAGAG AGTCACCTTC 
CTGGCGCTTC ACAATTACCT GGGTCTGACC AATGAACTCT TCAGTCATGA GATCCAGCCC 
CTACGCCTTT TCCCCAGCCC CGGCCTCCCG ACCCGAACCA GCCCTGTCCG TGGCTCTAAA 
CGGGTAGTCA GCACCTCAGG TACTGAGGAC ACCTCAAAGG AGACCTCTGG CCGGCCCCTG 
GACTCCATTG GAGCTCCTCT GCCTGGACCT GCAGGGGATC CCGTGAAGCC CACATCCTTA 
GAAGCACCCC CAGCCCCGTT ACTGAGCCGC TGCATCTCCA TGCCAGTAGA CATCTCAGGC 
TTGCAAGGTG GCCCTCGTTC TGATTTTGAC ATGGCCTATG AACGTGGACG GATATCTGTT 
TCTCTTCAAG AAGAGGCATC TGGGGGACCT CAGACAGCAT CCCCTAGGAC CCCCACTCAG 
GAGCTCCGGG AGCAGCCAGC GGGTGCCTGT GAATATAGCT ACTGTGAGGA TGAGTCAGCC 
ACAGGCGGAT GTCCCTTTGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCT 
GCAAAGAGAG AGCTAGCCAA GCTGATGCGG ATTGAGGACC CCTCTCTACT GAACAGCAGA 
GTCTTGCTAC ATCATGCCAA AGCCGGCACC ATCATAGCCC GCCAAGGGGA CCAGGATGTG 
AGCCTGCACT TTGTGCTCTG GGGCTGCCTG CACGTGTACC AGCGCATGAT TGACAAGGCT 
GAGGAAGTGT GCCTATTTGT GGCACAGCCA GGGGAGCTGG TGGGGCAACT GGCGGTGCTC 
ACTGGGGAAC CTCTCATCTT CACACTACGT GCCCAGAGAG ACTGCACCTT CCTGCGGATC 
TCCAAATCCC ACTTCTATGA GATCATGCGT GCACAACCCA GTGTGGTACT GAGTGCAGCT 
CACACGGTGG CTGCTAGAAT GTCCCCCTTC GTGCGCCAGA TGGACTTTGC CATTGACTGG 
ACAGCTGTGG AGGCTGGTCG TGCGCTCTAC AGGCAGGGAG ACCGCTCCGA CTGCACCTAC 
ATTGTACTCA ATGGACGTCT CCGTAGTGTC ATCCAACGAG GCAGTGGCAA GAAGGAGCTA 
GTTGGGGAGT ATGGTCGAGG GGATCTCATT GGCGTGGTGG AGGCACTGAC CCGGCAGCCA 
CGAGCCACCA CTGTACACGC AGTGCGAGAC ACTGAGCTGG CCAAGCTTCC CGAGGGCACC 
TTAGGCCACA TCAAACGTCG GTACCCACAG GTTGTGACCC GCCTTATTCA TCTGCTAAGC 
CAGAAAATTC TAGGCAATCT GCAGCAGTTG CAAGGACCCT TTCCAGGCTC CGGGCTCAGT 
GTTCCGCAGC ACTCGGAACT GACCAACCCA GCCAGCAATC TGTCTACTGT AGCTATCCTC 
CCTGTGTGTG CTGAGGTGCC CATGATGGCC TTTACTCTGG AACTGCAGCA TGCTCTGCAA 
GCTATTGGTC CCACGCTCCT CCTTAACAGT GACGTCATCC GGGCACTCCT GGGGGCTTCA 
GCACTGGATA GCATTCAAGA ATTCCGGCTG TCAGGGTGGT TGGCCCAGCA GGAAGATGCC 
CATCGCATTG TGCTCTACCA AACTGACACA TCCCTGACGC CTTGGACCGT CCGTTGTCTG 
CGCCAGGCCG ACTGCATCCT CATTGTGGGC CTGGGGGACC AGGAACCCAC TGTTGGCCAG 
CTGGAGCAAA TGTTAGAGAA CACTGCTGTC CGTGCCTTGA AGCAACTGGT TTTGCTGCAC 
CGGGAGGAAG GCCCTGGTCC CACGCGCACT GTAGAGTGGC TCAACATGCG CAGCTGGTGC 
TCAGGGCACC TGCACCTGCG CTGTCCTCGC CGCCTCTTCT CACGGCGCAG CCCAGCGAAA 
CTTCATGAGC TGTATGAGAA GGTTTTCTCC AGGCGTGCAG ACCGTCACAG CGACTTCTCC 
CGCTTGGCAC GAGTACTCAC AGGAAACACT ATTGCTCTGG TGCTGGGTGG GGGCGGAGCC 
AGAGGCTGCT CGCATATTGG GGTGCTGAAG GCATTAGAGG AGGCAGGAGT CCCAGTCGAC 
CTTGTGGGAG GCACATCCAT AGGTTCCTTC ATTGGGGCCT TGTATGCCGA GGAACGCAGC 
GCCAGCCGAA CTAAACAACG AGCCCGGGAG TGGGCCAAGA GCATGACTTC TGTACTGGAG 
CCTGTATTGG ACCTCACATA TCCTGTCACC TCCATGTTTA CTGGCTCAGC CTTTAACAGA 
AGTATCCACC GTGTCTTCCA GGATAAGCAG ATTGAGGACC TGTGGCTGCC TTACTTCAAT 
GTGACCACAG ACATCACTGC CTCTGCCATG CGCGTCCACA AAGATGGCTC CCTGTGGCGG 
TATGTACGTG CCAGCATGAC GCTCTCGGGC TACCTACCCC CGCTGTGCGA CCCAAAGGAT 
GGGCACCTGC TCATGGATGG TGGCTACATC AACAACCTGC CAGCGGATAT TGCCCGAAGC 
ATGGGAGCCA AAACGGTCAT TGCCATCGAC GTTGGAAGCC AGGATGAGAC AGATCTCAGC 
ACCTATGGGG ACAGCCTGTC TGGCTGGTGG TTGCTGTGGA AAAGGCTAAA CCCCTGGGCA 
GACAAGGTAA AGGTTCCAGA CATGGCTGAG ATCCAGTCCC GCTTGGCGTA CGTGTCCTGT 
GTGCGGCAGC TAGAAGTCGT CAAGTCCAGC TCCTATTGCG AGTACCTTCG TCCATCCATT 
GACTGCTTCA AGACCATGGA CTTTGGAAAG TTTGACCAGA TCTATGATGT GGGTTACCAG 
TATGGGAAGG CTGTCTTTGG AGGCTGGACC CGTGGTGAAG TCATTGAGAA AATGCTCACA 
GACCGGAGAT CTACAGACCT TAATGAGAGT CGCCGTGCAG ATATACTTGC CTTCCCAAGT 
TCTGGGTTCA CTGACTTGGC TGAGATTGTG TCCCGGATCG AGCCACCAAC AAGCTACGTC 
TCTGATGGCT GTGCTGATGG GGAGGAGTCG GATTGCTTGA CAGAGTATGA GGAAGATGCA 
GGACCAGACT GCTCAAGAGA TGAAGGGGGC TCCCCTGAGG GTGCCAGCCC TAGCACTGCC 
TCAGAGGTGG AAGAAGAGAA GTCCACACTC CGGCAACGAC GCTTTCTGCC TCAGGAGACT 
CCCAGCTCAG TCGCAGATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508825.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508888.2 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X4, mRNA.

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CloneID	OMu10290
Clone ID Related Accession (Same CDS sequence)	XM_006508825.3 , NM_015801.2
Accession Version	XM_006508825.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508825.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC CACTGCAAAC CGGAATGGTG CTGGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGCTGGTCA CCGCCGTGCT CATCCTCTTG GTGGTGCGAA GGCTGCGGGT GCAGAAAACT 
CCAGCCCCGG AGGGTCCCCG GTATCGGTTC CGGAAGCGGG ACAAAGTGCT TTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAGTCC ACTTCTTCCC TGGTGGACAC ATCAGTCTCC 
ACCACTTCCC GGCCCCGCAT GAAGAAGAAA CTGAAGATGC TTAACATTGC CAAGAAGATC 
TTACGTATCC AAAAGGAGAC TCCGACCCTA CAACGGAAGG AGCCCCCACC TTCAGTGCTG 
GAGGCTGACC TCACAGAGGG AGACCTGGCC AATTCCCACC TACCCTCTGA GGTACTCTAC 
ATGCTTAAGA ATGTTCGGGT GCTAGGTCAC TTTGAGAAGC CGCTCTTCCT GGAGCTCTGC 
CGGCACATGG TCTTCCAGCG TCTCGGGCAG GGGGACTATG TTTTTCGGCC CGGTCAACCA 
GATGCCAGCA TCTATGTGGT TCAAGATGGG CTGCTGGAGC TCTGCCTACC AGGGCCTGAT 
GGGAAGGAGT GTGTGGTGAA GGAGGTGGTT CCTGGAGACA GCGTCAACAG CCTTCTGAGC 
ATCCTTGATG TCATCACGGG TCACCAGCAT CCCCAGCGGA CTGTGTCAGC CAGGGCTGCA 
CGGGACTCCA CAGTGCTGAG ACTCCCAGTA GAAGCCTTCT CTGCTGTCTT CACCAAGTAC 
CCTGAGAGTT TGGTGCGAGT GGTACAGATC ATCATGGTAA GGCTGCAGAG AGTCACCTTC 
CTGGCGCTTC ACAATTACCT GGGTCTGACC AATGAACTCT TCAGTCATGA GATCCAGCCC 
CTACGCCTTT TCCCCAGCCC CGGCCTCCCG ACCCGAACCA GCCCTGTCCG TGGCTCTAAA 
CGGGTAGTCA GCACCTCAGG TACTGAGGAC ACCTCAAAGG AGACCTCTGG CCGGCCCCTG 
GACTCCATTG GAGCTCCTCT GCCTGGACCT GCAGGGGATC CCGTGAAGCC CACATCCTTA 
GAAGCACCCC CAGCCCCGTT ACTGAGCCGC TGCATCTCCA TGCCAGTAGA CATCTCAGGC 
TTGCAAGGTG GCCCTCGTTC TGATTTTGAC ATGGCCTATG AACGTGGACG GATATCTGTT 
TCTCTTCAAG AAGAGGCATC TGGGGGACCT CAGACAGCAT CCCCTAGGAC CCCCACTCAG 
GAGCTCCGGG AGCAGCCAGC GGGTGCCTGT GAATATAGCT ACTGTGAGGA TGAGTCAGCC 
ACAGGCGGAT GTCCCTTTGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCT 
GCAAAGAGAG AGCTAGCCAA GCTGATGCGG ATTGAGGACC CCTCTCTACT GAACAGCAGA 
GTCTTGCTAC ATCATGCCAA AGCCGGCACC ATCATAGCCC GCCAAGGGGA CCAGGATGTG 
AGCCTGCACT TTGTGCTCTG GGGCTGCCTG CACGTGTACC AGCGCATGAT TGACAAGGCT 
GAGGAAGTGT GCCTATTTGT GGCACAGCCA GGGGAGCTGG TGGGGCAACT GGCGGTGCTC 
ACTGGGGAAC CTCTCATCTT CACACTACGT GCCCAGAGAG ACTGCACCTT CCTGCGGATC 
TCCAAATCCC ACTTCTATGA GATCATGCGT GCACAACCCA GTGTGGTACT GAGTGCAGCT 
CACACGGTGG CTGCTAGAAT GTCCCCCTTC GTGCGCCAGA TGGACTTTGC CATTGACTGG 
ACAGCTGTGG AGGCTGGTCG TGCGCTCTAC AGGCAGGGAG ACCGCTCCGA CTGCACCTAC 
ATTGTACTCA ATGGACGTCT CCGTAGTGTC ATCCAACGAG GCAGTGGCAA GAAGGAGCTA 
GTTGGGGAGT ATGGTCGAGG GGATCTCATT GGCGTGGTGG AGGCACTGAC CCGGCAGCCA 
CGAGCCACCA CTGTACACGC AGTGCGAGAC ACTGAGCTGG CCAAGCTTCC CGAGGGCACC 
TTAGGCCACA TCAAACGTCG GTACCCACAG GTTGTGACCC GCCTTATTCA TCTGCTAAGC 
CAGAAAATTC TAGGCAATCT GCAGCAGTTG CAAGGACCCT TTCCAGGCTC CGGGCTCAGT 
GTTCCGCAGC ACTCGGAACT GACCAACCCA GCCAGCAATC TGTCTACTGT AGCTATCCTC 
CCTGTGTGTG CTGAGGTGCC CATGATGGCC TTTACTCTGG AACTGCAGCA TGCTCTGCAA 
GCTATTGGTC CCACGCTCCT CCTTAACAGT GACGTCATCC GGGCACTCCT GGGGGCTTCA 
GCACTGGATA GCATTCAAGA ATTCCGGCTG TCAGGGTGGT TGGCCCAGCA GGAAGATGCC 
CATCGCATTG TGCTCTACCA AACTGACACA TCCCTGACGC CTTGGACCGT CCGTTGTCTG 
CGCCAGGCCG ACTGCATCCT CATTGTGGGC CTGGGGGACC AGGAACCCAC TGTTGGCCAG 
CTGGAGCAAA TGTTAGAGAA CACTGCTGTC CGTGCCTTGA AGCAACTGGT TTTGCTGCAC 
CGGGAGGAAG GCCCTGGTCC CACGCGCACT GTAGAGTGGC TCAACATGCG CAGCTGGTGC 
TCAGGGCACC TGCACCTGCG CTGTCCTCGC CGCCTCTTCT CACGGCGCAG CCCAGCGAAA 
CTTCATGAGC TGTATGAGAA GGTTTTCTCC AGGCGTGCAG ACCGTCACAG CGACTTCTCC 
CGCTTGGCAC GAGTACTCAC AGGAAACACT ATTGCTCTGG TGCTGGGTGG GGGCGGAGCC 
AGAGGCTGCT CGCATATTGG GGTGCTGAAG GCATTAGAGG AGGCAGGAGT CCCAGTCGAC 
CTTGTGGGAG GCACATCCAT AGGTTCCTTC ATTGGGGCCT TGTATGCCGA GGAACGCAGC 
GCCAGCCGAA CTAAACAACG AGCCCGGGAG TGGGCCAAGA GCATGACTTC TGTACTGGAG 
CCTGTATTGG ACCTCACATA TCCTGTCACC TCCATGTTTA CTGGCTCAGC CTTTAACAGA 
AGTATCCACC GTGTCTTCCA GGATAAGCAG ATTGAGGACC TGTGGCTGCC TTACTTCAAT 
GTGACCACAG ACATCACTGC CTCTGCCATG CGCGTCCACA AAGATGGCTC CCTGTGGCGG 
TATGTACGTG CCAGCATGAC GCTCTCGGGC TACCTACCCC CGCTGTGCGA CCCAAAGGAT 
GGGCACCTGC TCATGGATGG TGGCTACATC AACAACCTGC CAGCGGATAT TGCCCGAAGC 
ATGGGAGCCA AAACGGTCAT TGCCATCGAC GTTGGAAGCC AGGATGAGAC AGATCTCAGC 
ACCTATGGGG ACAGCCTGTC TGGCTGGTGG TTGCTGTGGA AAAGGCTAAA CCCCTGGGCA 
GACAAGGTAA AGGTTCCAGA CATGGCTGAG ATCCAGTCCC GCTTGGCGTA CGTGTCCTGT 
GTGCGGCAGC TAGAAGTCGT CAAGTCCAGC TCCTATTGCG AGTACCTTCG TCCATCCATT 
GACTGCTTCA AGACCATGGA CTTTGGAAAG TTTGACCAGA TCTATGATGT GGGTTACCAG 
TATGGGAAGG CTGTCTTTGG AGGCTGGACC CGTGGTGAAG TCATTGAGAA AATGCTCACA 
GACCGGAGAT CTACAGACCT TAATGAGAGT CGCCGTGCAG ATATACTTGC CTTCCCAAGT 
TCTGGGTTCA CTGACTTGGC TGAGATTGTG TCCCGGATCG AGCCACCAAC AAGCTACGTC 
TCTGATGGCT GTGCTGATGG GGAGGAGTCG GATTGCTTGA CAGAGTATGA GGAAGATGCA 
GGACCAGACT GCTCAAGAGA TGAAGGGGGC TCCCCTGAGG GTGCCAGCCC TAGCACTGCC 
TCAGAGGTGG AAGAAGAGAA GTCCACACTC CGGCAACGAC GCTTTCTGCC TCAGGAGACT 
CCCAGCTCAG TCGCAGATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508888.2
CDS	65..4048
Translation	MEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508825.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508825.3

ORF Insert Sequence:

ATGGAGGCCC CACTGCAAAC CGGAATGGTG CTGGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGCTGGTCA CCGCCGTGCT CATCCTCTTG GTGGTGCGAA GGCTGCGGGT GCAGAAAACT 
CCAGCCCCGG AGGGTCCCCG GTATCGGTTC CGGAAGCGGG ACAAAGTGCT TTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAGTCC ACTTCTTCCC TGGTGGACAC ATCAGTCTCC 
ACCACTTCCC GGCCCCGCAT GAAGAAGAAA CTGAAGATGC TTAACATTGC CAAGAAGATC 
TTACGTATCC AAAAGGAGAC TCCGACCCTA CAACGGAAGG AGCCCCCACC TTCAGTGCTG 
GAGGCTGACC TCACAGAGGG AGACCTGGCC AATTCCCACC TACCCTCTGA GGTACTCTAC 
ATGCTTAAGA ATGTTCGGGT GCTAGGTCAC TTTGAGAAGC CGCTCTTCCT GGAGCTCTGC 
CGGCACATGG TCTTCCAGCG TCTCGGGCAG GGGGACTATG TTTTTCGGCC CGGTCAACCA 
GATGCCAGCA TCTATGTGGT TCAAGATGGG CTGCTGGAGC TCTGCCTACC AGGGCCTGAT 
GGGAAGGAGT GTGTGGTGAA GGAGGTGGTT CCTGGAGACA GCGTCAACAG CCTTCTGAGC 
ATCCTTGATG TCATCACGGG TCACCAGCAT CCCCAGCGGA CTGTGTCAGC CAGGGCTGCA 
CGGGACTCCA CAGTGCTGAG ACTCCCAGTA GAAGCCTTCT CTGCTGTCTT CACCAAGTAC 
CCTGAGAGTT TGGTGCGAGT GGTACAGATC ATCATGGTAA GGCTGCAGAG AGTCACCTTC 
CTGGCGCTTC ACAATTACCT GGGTCTGACC AATGAACTCT TCAGTCATGA GATCCAGCCC 
CTACGCCTTT TCCCCAGCCC CGGCCTCCCG ACCCGAACCA GCCCTGTCCG TGGCTCTAAA 
CGGGTAGTCA GCACCTCAGG TACTGAGGAC ACCTCAAAGG AGACCTCTGG CCGGCCCCTG 
GACTCCATTG GAGCTCCTCT GCCTGGACCT GCAGGGGATC CCGTGAAGCC CACATCCTTA 
GAAGCACCCC CAGCCCCGTT ACTGAGCCGC TGCATCTCCA TGCCAGTAGA CATCTCAGGC 
TTGCAAGGTG GCCCTCGTTC TGATTTTGAC ATGGCCTATG AACGTGGACG GATATCTGTT 
TCTCTTCAAG AAGAGGCATC TGGGGGACCT CAGACAGCAT CCCCTAGGAC CCCCACTCAG 
GAGCTCCGGG AGCAGCCAGC GGGTGCCTGT GAATATAGCT ACTGTGAGGA TGAGTCAGCC 
ACAGGCGGAT GTCCCTTTGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCT 
GCAAAGAGAG AGCTAGCCAA GCTGATGCGG ATTGAGGACC CCTCTCTACT GAACAGCAGA 
GTCTTGCTAC ATCATGCCAA AGCCGGCACC ATCATAGCCC GCCAAGGGGA CCAGGATGTG 
AGCCTGCACT TTGTGCTCTG GGGCTGCCTG CACGTGTACC AGCGCATGAT TGACAAGGCT 
GAGGAAGTGT GCCTATTTGT GGCACAGCCA GGGGAGCTGG TGGGGCAACT GGCGGTGCTC 
ACTGGGGAAC CTCTCATCTT CACACTACGT GCCCAGAGAG ACTGCACCTT CCTGCGGATC 
TCCAAATCCC ACTTCTATGA GATCATGCGT GCACAACCCA GTGTGGTACT GAGTGCAGCT 
CACACGGTGG CTGCTAGAAT GTCCCCCTTC GTGCGCCAGA TGGACTTTGC CATTGACTGG 
ACAGCTGTGG AGGCTGGTCG TGCGCTCTAC AGGCAGGGAG ACCGCTCCGA CTGCACCTAC 
ATTGTACTCA ATGGACGTCT CCGTAGTGTC ATCCAACGAG GCAGTGGCAA GAAGGAGCTA 
GTTGGGGAGT ATGGTCGAGG GGATCTCATT GGCGTGGTGG AGGCACTGAC CCGGCAGCCA 
CGAGCCACCA CTGTACACGC AGTGCGAGAC ACTGAGCTGG CCAAGCTTCC CGAGGGCACC 
TTAGGCCACA TCAAACGTCG GTACCCACAG GTTGTGACCC GCCTTATTCA TCTGCTAAGC 
CAGAAAATTC TAGGCAATCT GCAGCAGTTG CAAGGACCCT TTCCAGGCTC CGGGCTCAGT 
GTTCCGCAGC ACTCGGAACT GACCAACCCA GCCAGCAATC TGTCTACTGT AGCTATCCTC 
CCTGTGTGTG CTGAGGTGCC CATGATGGCC TTTACTCTGG AACTGCAGCA TGCTCTGCAA 
GCTATTGGTC CCACGCTCCT CCTTAACAGT GACGTCATCC GGGCACTCCT GGGGGCTTCA 
GCACTGGATA GCATTCAAGA ATTCCGGCTG TCAGGGTGGT TGGCCCAGCA GGAAGATGCC 
CATCGCATTG TGCTCTACCA AACTGACACA TCCCTGACGC CTTGGACCGT CCGTTGTCTG 
CGCCAGGCCG ACTGCATCCT CATTGTGGGC CTGGGGGACC AGGAACCCAC TGTTGGCCAG 
CTGGAGCAAA TGTTAGAGAA CACTGCTGTC CGTGCCTTGA AGCAACTGGT TTTGCTGCAC 
CGGGAGGAAG GCCCTGGTCC CACGCGCACT GTAGAGTGGC TCAACATGCG CAGCTGGTGC 
TCAGGGCACC TGCACCTGCG CTGTCCTCGC CGCCTCTTCT CACGGCGCAG CCCAGCGAAA 
CTTCATGAGC TGTATGAGAA GGTTTTCTCC AGGCGTGCAG ACCGTCACAG CGACTTCTCC 
CGCTTGGCAC GAGTACTCAC AGGAAACACT ATTGCTCTGG TGCTGGGTGG GGGCGGAGCC 
AGAGGCTGCT CGCATATTGG GGTGCTGAAG GCATTAGAGG AGGCAGGAGT CCCAGTCGAC 
CTTGTGGGAG GCACATCCAT AGGTTCCTTC ATTGGGGCCT TGTATGCCGA GGAACGCAGC 
GCCAGCCGAA CTAAACAACG AGCCCGGGAG TGGGCCAAGA GCATGACTTC TGTACTGGAG 
CCTGTATTGG ACCTCACATA TCCTGTCACC TCCATGTTTA CTGGCTCAGC CTTTAACAGA 
AGTATCCACC GTGTCTTCCA GGATAAGCAG ATTGAGGACC TGTGGCTGCC TTACTTCAAT 
GTGACCACAG ACATCACTGC CTCTGCCATG CGCGTCCACA AAGATGGCTC CCTGTGGCGG 
TATGTACGTG CCAGCATGAC GCTCTCGGGC TACCTACCCC CGCTGTGCGA CCCAAAGGAT 
GGGCACCTGC TCATGGATGG TGGCTACATC AACAACCTGC CAGCGGATAT TGCCCGAAGC 
ATGGGAGCCA AAACGGTCAT TGCCATCGAC GTTGGAAGCC AGGATGAGAC AGATCTCAGC 
ACCTATGGGG ACAGCCTGTC TGGCTGGTGG TTGCTGTGGA AAAGGCTAAA CCCCTGGGCA 
GACAAGGTAA AGGTTCCAGA CATGGCTGAG ATCCAGTCCC GCTTGGCGTA CGTGTCCTGT 
GTGCGGCAGC TAGAAGTCGT CAAGTCCAGC TCCTATTGCG AGTACCTTCG TCCATCCATT 
GACTGCTTCA AGACCATGGA CTTTGGAAAG TTTGACCAGA TCTATGATGT GGGTTACCAG 
TATGGGAAGG CTGTCTTTGG AGGCTGGACC CGTGGTGAAG TCATTGAGAA AATGCTCACA 
GACCGGAGAT CTACAGACCT TAATGAGAGT CGCCGTGCAG ATATACTTGC CTTCCCAAGT 
TCTGGGTTCA CTGACTTGGC TGAGATTGTG TCCCGGATCG AGCCACCAAC AAGCTACGTC 
TCTGATGGCT GTGCTGATGG GGAGGAGTCG GATTGCTTGA CAGAGTATGA GGAAGATGCA 
GGACCAGACT GCTCAAGAGA TGAAGGGGGC TCCCCTGAGG GTGCCAGCCC TAGCACTGCC 
TCAGAGGTGG AAGAAGAGAA GTCCACACTC CGGCAACGAC GCTTTCTGCC TCAGGAGACT 
CCCAGCTCAG TCGCAGATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508832.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508895.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu40428
Clone ID Related Accession (Same CDS sequence)	XM_006508832.3 , XM_006508832.4
Accession Version	XM_006508832.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTGCC CAGCCTGTGC CGTTCGTTCC GCAGAAACTC CAGCCCCGGA GGGTCCCCGG 
TATCGGTTCC GGAAGCGGGA CAAAGTGCTT TTCTATGGCC GGAAGATTAT GCGGAAGGTG 
TCACAGTCCA CTTCTTCCCT GGTGGACACA TCAGTCTCCA CCACTTCCCG GCCCCGCATG 
AAGAAGAAAC TGAAGATGCT TAACATTGCC AAGAAGATCT TACGTATCCA AAAGGAGACT 
CCGACCCTAC AACGGAAGGA GCCCCCACCT TCAGTGCTGG AGGCTGACCT CACAGAGGGA 
GACCTGGCCA ATTCCCACCT ACCCTCTGAG GTACTCTACA TGCTTAAGAA TGTTCGGGTG 
CTAGGTCACT TTGAGAAGCC GCTCTTCCTG GAGCTCTGCC GGCACATGGT CTTCCAGCGT 
CTCGGGCAGG GGGACTATGT TTTTCGGCCC GGTCAACCAG ATGCCAGCAT CTATGTGGTT 
CAAGATGGGC TGCTGGAGCT CTGCCTACCA GGGCCTGATG GGAAGGAGTG TGTGGTGAAG 
GAGGTGGTTC CTGGAGACAG CGTCAACAGC CTTCTGAGCA TCCTTGATGT CATCACGGGT 
CACCAGCATC CCCAGCGGAC TGTGTCAGCC AGGGCTGCAC GGGACTCCAC AGTGCTGAGA 
CTCCCAGTAG AAGCCTTCTC TGCTGTCTTC ACCAAGTACC CTGAGAGTTT GGTGCGAGTG 
GTACAGATCA TCATGGTAAG GCTGCAGAGA GTCACCTTCC TGGCGCTTCA CAATTACCTG 
GGTCTGACCA ATGAACTCTT CAGTCATGAG ATCCAGCCCC TACGCCTTTT CCCCAGCCCC 
GGCCTCCCGA CCCGAACCAG CCCTGTCCGT GGCTCTAAAC GGGTAGTCAG CACCTCAGGT 
ACTGAGGACA CCTCAAAGGA GACCTCTGGC CGGCCCCTGG ACTCCATTGG AGCTCCTCTG 
CCTGGACCTG CAGGGGATCC CGTGAAGCCC ACATCCTTAG AAGCACCCCC AGCCCCGTTA 
CTGAGCCGCT GCATCTCCAT GCCAGTAGAC ATCTCAGGCT TGCAAGGTGG CCCTCGTTCT 
GATTTTGACA TGGCCTATGA ACGTGGACGG ATATCTGTTT CTCTTCAAGA AGAGGCATCT 
GGGGGACCTC AGACAGCATC CCCTAGGACC CCCACTCAGG AGCTCCGGGA GCAGCCAGCG 
GGTGCCTGTG AATATAGCTA CTGTGAGGAT GAGTCAGCCA CAGGCGGATG TCCCTTTGGG 
CCCTACCAGG GCCGCCAGAC AAGCAGCATC TTTGAGGCTG CAAAGAGAGA GCTAGCCAAG 
CTGATGCGGA TTGAGGACCC CTCTCTACTG AACAGCAGAG TCTTGCTACA TCATGCCAAA 
GCCGGCACCA TCATAGCCCG CCAAGGGGAC CAGGATGTGA GCCTGCACTT TGTGCTCTGG 
GGCTGCCTGC ACGTGTACCA GCGCATGATT GACAAGGCTG AGGAAGTGTG CCTATTTGTG 
GCACAGCCAG GGGAGCTGGT GGGGCAACTG GCGGTGCTCA CTGGGGAACC TCTCATCTTC 
ACACTACGTG CCCAGAGAGA CTGCACCTTC CTGCGGATCT CCAAATCCCA CTTCTATGAG 
ATCATGCGTG CACAACCCAG TGTGGTACTG AGTGCAGCTC ACACGGTGGC TGCTAGAATG 
TCCCCCTTCG TGCGCCAGAT GGACTTTGCC ATTGACTGGA CAGCTGTGGA GGCTGGTCGT 
GCGCTCTACA GGCAGGGAGA CCGCTCCGAC TGCACCTACA TTGTACTCAA TGGACGTCTC 
CGTAGTGTCA TCCAACGAGG CAGTGGCAAG AAGGAGCTAG TTGGGGAGTA TGGTCGAGGG 
GATCTCATTG GCGTGGTGGA GGCACTGACC CGGCAGCCAC GAGCCACCAC TGTACACGCA 
GTGCGAGACA CTGAGCTGGC CAAGCTTCCC GAGGGCACCT TAGGCCACAT CAAACGTCGG 
TACCCACAGG TTGTGACCCG CCTTATTCAT CTGCTAAGCC AGAAAATTCT AGGCAATCTG 
CAGCAGTTGC AAGGACCCTT TCCAGGCTCC GGGCTCAGTG TTCCGCAGCA CTCGGAACTG 
ACCAACCCAG CCAGCAATCT GTCTACTGTA GCTATCCTCC CTGTGTGTGC TGAGGTGCCC 
ATGATGGCCT TTACTCTGGA ACTGCAGCAT GCTCTGCAAG CTATTGGTCC CACGCTCCTC 
CTTAACAGTG ACGTCATCCG GGCACTCCTG GGGGCTTCAG CACTGGATAG CATTCAAGAA 
TTCCGGCTGT CAGGGTGGTT GGCCCAGCAG GAAGATGCCC ATCGCATTGT GCTCTACCAA 
ACTGACACAT CCCTGACGCC TTGGACCGTC CGTTGTCTGC GCCAGGCCGA CTGCATCCTC 
ATTGTGGGCC TGGGGGACCA GGAACCCACT GTTGGCCAGC TGGAGCAAAT GTTAGAGAAC 
ACTGCTGTCC GTGCCTTGAA GCAACTGGTT TTGCTGCACC GGGAGGAAGG CCCTGGTCCC 
ACGCGCACTG TAGAGTGGCT CAACATGCGC AGCTGGTGCT CAGGGCACCT GCACCTGCGC 
TGTCCTCGCC GCCTCTTCTC ACGGCGCAGC CCAGCGAAAC TTCATGAGCT GTATGAGAAG 
GTTTTCTCCA GGCGTGCAGA CCGTCACAGC GACTTCTCCC GCTTGGCACG AGTACTCACA 
GGAAACACTA TTGCTCTGGT GCTGGGTGGG GGCGGAGCCA GAGGCTGCTC GCATATTGGG 
GTGCTGAAGG CATTAGAGGA GGCAGGAGTC CCAGTCGACC TTGTGGGAGG CACATCCATA 
GGTTCCTTCA TTGGGGCCTT GTATGCCGAG GAACGCAGCG CCAGCCGAAC TAAACAACGA 
GCCCGGGAGT GGGCCAAGAG CATGACTTCT GTACTGGAGC CTGTATTGGA CCTCACATAT 
CCTGTCACCT CCATGTTTAC TGGCTCAGCC TTTAACAGAA GTATCCACCG TGTCTTCCAG 
GATAAGCAGA TTGAGGACCT GTGGCTGCCT TACTTCAATG TGACCACAGA CATCACTGCC 
TCTGCCATGC GCGTCCACAA AGATGGCTCC CTGTGGCGGT ATGTACGTGC CAGCATGACG 
CTCTCGGGCT ACCTACCCCC GCTGTGCGAC CCAAAGGATG GGCACCTGCT CATGGATGGT 
GGCTACATCA ACAACCTGCC AGCGGATATT GCCCGAAGCA TGGGAGCCAA AACGGTCATT 
GCCATCGACG TTGGAAGCCA GGATGAGACA GATCTCAGCA CCTATGGGGA CAGCCTGTCT 
GGCTGGTGGT TGCTGTGGAA AAGGCTAAAC CCCTGGGCAG ACAAGGTAAA GGTTCCAGAC 
ATGGCTGAGA TCCAGTCCCG CTTGGCGTAC GTGTCCTGTG TGCGGCAGCT AGAAGTCGTC 
AAGTCCAGCT CCTATTGCGA GTACCTTCGT CCATCCATTG ACTGCTTCAA GACCATGGAC 
TTTGGAAAGT TTGACCAGAT CTATGATGTG GGTTACCAGT ATGGGAAGGC TGTCTTTGGA 
GGCTGGACCC GTGGTGAAGT CATTGAGAAA ATGCTCACAG ACCGGAGATC TACAGACCTT 
AATGAGAGTC GCCGTGCAGA TATACTTGCC TTCCCAAGTT CTGGGTTCAC TGACTTGGCT 
GAGATTGTGT CCCGGATCGA GCCACCAACA AGCTACGTCT CTGATGGCTG TGCTGATGGG 
GAGGAGTCGG ATTGCTTGAC AGAGTATGAG GAAGATGCAG GACCAGACTG CTCAAGAGAT 
GAAGGGGGCT CCCCTGAGGG TGCCAGCCCT AGCACTGCCT CAGAGGTGGA AGAAGAGAAG 
TCCACACTCC GGCAACGACG CTTTCTGCCT CAGGAGACTC CCAGCTCAGT CGCAGATGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508895.1
CDS	237..4139
Translation	MRCPACAVRSAETPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508832.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508832.3

ORF Insert Sequence:

ATGCGTTGCC CAGCCTGTGC CGTTCGTTCC GCAGAAACTC CAGCCCCGGA GGGTCCCCGG 
TATCGGTTCC GGAAGCGGGA CAAAGTGCTT TTCTATGGCC GGAAGATTAT GCGGAAGGTG 
TCACAGTCCA CTTCTTCCCT GGTGGACACA TCAGTCTCCA CCACTTCCCG GCCCCGCATG 
AAGAAGAAAC TGAAGATGCT TAACATTGCC AAGAAGATCT TACGTATCCA AAAGGAGACT 
CCGACCCTAC AACGGAAGGA GCCCCCACCT TCAGTGCTGG AGGCTGACCT CACAGAGGGA 
GACCTGGCCA ATTCCCACCT ACCCTCTGAG GTACTCTACA TGCTTAAGAA TGTTCGGGTG 
CTAGGTCACT TTGAGAAGCC GCTCTTCCTG GAGCTCTGCC GGCACATGGT CTTCCAGCGT 
CTCGGGCAGG GGGACTATGT TTTTCGGCCC GGTCAACCAG ATGCCAGCAT CTATGTGGTT 
CAAGATGGGC TGCTGGAGCT CTGCCTACCA GGGCCTGATG GGAAGGAGTG TGTGGTGAAG 
GAGGTGGTTC CTGGAGACAG CGTCAACAGC CTTCTGAGCA TCCTTGATGT CATCACGGGT 
CACCAGCATC CCCAGCGGAC TGTGTCAGCC AGGGCTGCAC GGGACTCCAC AGTGCTGAGA 
CTCCCAGTAG AAGCCTTCTC TGCTGTCTTC ACCAAGTACC CTGAGAGTTT GGTGCGAGTG 
GTACAGATCA TCATGGTAAG GCTGCAGAGA GTCACCTTCC TGGCGCTTCA CAATTACCTG 
GGTCTGACCA ATGAACTCTT CAGTCATGAG ATCCAGCCCC TACGCCTTTT CCCCAGCCCC 
GGCCTCCCGA CCCGAACCAG CCCTGTCCGT GGCTCTAAAC GGGTAGTCAG CACCTCAGGT 
ACTGAGGACA CCTCAAAGGA GACCTCTGGC CGGCCCCTGG ACTCCATTGG AGCTCCTCTG 
CCTGGACCTG CAGGGGATCC CGTGAAGCCC ACATCCTTAG AAGCACCCCC AGCCCCGTTA 
CTGAGCCGCT GCATCTCCAT GCCAGTAGAC ATCTCAGGCT TGCAAGGTGG CCCTCGTTCT 
GATTTTGACA TGGCCTATGA ACGTGGACGG ATATCTGTTT CTCTTCAAGA AGAGGCATCT 
GGGGGACCTC AGACAGCATC CCCTAGGACC CCCACTCAGG AGCTCCGGGA GCAGCCAGCG 
GGTGCCTGTG AATATAGCTA CTGTGAGGAT GAGTCAGCCA CAGGCGGATG TCCCTTTGGG 
CCCTACCAGG GCCGCCAGAC AAGCAGCATC TTTGAGGCTG CAAAGAGAGA GCTAGCCAAG 
CTGATGCGGA TTGAGGACCC CTCTCTACTG AACAGCAGAG TCTTGCTACA TCATGCCAAA 
GCCGGCACCA TCATAGCCCG CCAAGGGGAC CAGGATGTGA GCCTGCACTT TGTGCTCTGG 
GGCTGCCTGC ACGTGTACCA GCGCATGATT GACAAGGCTG AGGAAGTGTG CCTATTTGTG 
GCACAGCCAG GGGAGCTGGT GGGGCAACTG GCGGTGCTCA CTGGGGAACC TCTCATCTTC 
ACACTACGTG CCCAGAGAGA CTGCACCTTC CTGCGGATCT CCAAATCCCA CTTCTATGAG 
ATCATGCGTG CACAACCCAG TGTGGTACTG AGTGCAGCTC ACACGGTGGC TGCTAGAATG 
TCCCCCTTCG TGCGCCAGAT GGACTTTGCC ATTGACTGGA CAGCTGTGGA GGCTGGTCGT 
GCGCTCTACA GGCAGGGAGA CCGCTCCGAC TGCACCTACA TTGTACTCAA TGGACGTCTC 
CGTAGTGTCA TCCAACGAGG CAGTGGCAAG AAGGAGCTAG TTGGGGAGTA TGGTCGAGGG 
GATCTCATTG GCGTGGTGGA GGCACTGACC CGGCAGCCAC GAGCCACCAC TGTACACGCA 
GTGCGAGACA CTGAGCTGGC CAAGCTTCCC GAGGGCACCT TAGGCCACAT CAAACGTCGG 
TACCCACAGG TTGTGACCCG CCTTATTCAT CTGCTAAGCC AGAAAATTCT AGGCAATCTG 
CAGCAGTTGC AAGGACCCTT TCCAGGCTCC GGGCTCAGTG TTCCGCAGCA CTCGGAACTG 
ACCAACCCAG CCAGCAATCT GTCTACTGTA GCTATCCTCC CTGTGTGTGC TGAGGTGCCC 
ATGATGGCCT TTACTCTGGA ACTGCAGCAT GCTCTGCAAG CTATTGGTCC CACGCTCCTC 
CTTAACAGTG ACGTCATCCG GGCACTCCTG GGGGCTTCAG CACTGGATAG CATTCAAGAA 
TTCCGGCTGT CAGGGTGGTT GGCCCAGCAG GAAGATGCCC ATCGCATTGT GCTCTACCAA 
ACTGACACAT CCCTGACGCC TTGGACCGTC CGTTGTCTGC GCCAGGCCGA CTGCATCCTC 
ATTGTGGGCC TGGGGGACCA GGAACCCACT GTTGGCCAGC TGGAGCAAAT GTTAGAGAAC 
ACTGCTGTCC GTGCCTTGAA GCAACTGGTT TTGCTGCACC GGGAGGAAGG CCCTGGTCCC 
ACGCGCACTG TAGAGTGGCT CAACATGCGC AGCTGGTGCT CAGGGCACCT GCACCTGCGC 
TGTCCTCGCC GCCTCTTCTC ACGGCGCAGC CCAGCGAAAC TTCATGAGCT GTATGAGAAG 
GTTTTCTCCA GGCGTGCAGA CCGTCACAGC GACTTCTCCC GCTTGGCACG AGTACTCACA 
GGAAACACTA TTGCTCTGGT GCTGGGTGGG GGCGGAGCCA GAGGCTGCTC GCATATTGGG 
GTGCTGAAGG CATTAGAGGA GGCAGGAGTC CCAGTCGACC TTGTGGGAGG CACATCCATA 
GGTTCCTTCA TTGGGGCCTT GTATGCCGAG GAACGCAGCG CCAGCCGAAC TAAACAACGA 
GCCCGGGAGT GGGCCAAGAG CATGACTTCT GTACTGGAGC CTGTATTGGA CCTCACATAT 
CCTGTCACCT CCATGTTTAC TGGCTCAGCC TTTAACAGAA GTATCCACCG TGTCTTCCAG 
GATAAGCAGA TTGAGGACCT GTGGCTGCCT TACTTCAATG TGACCACAGA CATCACTGCC 
TCTGCCATGC GCGTCCACAA AGATGGCTCC CTGTGGCGGT ATGTACGTGC CAGCATGACG 
CTCTCGGGCT ACCTACCCCC GCTGTGCGAC CCAAAGGATG GGCACCTGCT CATGGATGGT 
GGCTACATCA ACAACCTGCC AGCGGATATT GCCCGAAGCA TGGGAGCCAA AACGGTCATT 
GCCATCGACG TTGGAAGCCA GGATGAGACA GATCTCAGCA CCTATGGGGA CAGCCTGTCT 
GGCTGGTGGT TGCTGTGGAA AAGGCTAAAC CCCTGGGCAG ACAAGGTAAA GGTTCCAGAC 
ATGGCTGAGA TCCAGTCCCG CTTGGCGTAC GTGTCCTGTG TGCGGCAGCT AGAAGTCGTC 
AAGTCCAGCT CCTATTGCGA GTACCTTCGT CCATCCATTG ACTGCTTCAA GACCATGGAC 
TTTGGAAAGT TTGACCAGAT CTATGATGTG GGTTACCAGT ATGGGAAGGC TGTCTTTGGA 
GGCTGGACCC GTGGTGAAGT CATTGAGAAA ATGCTCACAG ACCGGAGATC TACAGACCTT 
AATGAGAGTC GCCGTGCAGA TATACTTGCC TTCCCAAGTT CTGGGTTCAC TGACTTGGCT 
GAGATTGTGT CCCGGATCGA GCCACCAACA AGCTACGTCT CTGATGGCTG TGCTGATGGG 
GAGGAGTCGG ATTGCTTGAC AGAGTATGAG GAAGATGCAG GACCAGACTG CTCAAGAGAT 
GAAGGGGGCT CCCCTGAGGG TGCCAGCCCT AGCACTGCCT CAGAGGTGGA AGAAGAGAAG 
TCCACACTCC GGCAACGACG CTTTCTGCCT CAGGAGACTC CCAGCTCAGT CGCAGATGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_011242066.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240368.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu69375
Clone ID Related Accession (Same CDS sequence)	XM_011242066.2 , XM_011242066.3
Accession Version	XM_011242066.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242066.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTGCC CAGCCTGTGC CGTTCGTTCC GCAGGTGCTG GGCGTGATGA TCGGGGCCGG 
AGTGGCGGTG CTGGTCACCG CCGTGCTCAT CCTCTTGGTG AAACTCCAGC CCCGGAGGGT 
CCCCGGTATC GGTTCCGGAA GCGGGACAAA GTGCTTTTCT ATGGCCGGAA GATTATGCGG 
AAGGTGTCAC AGTCCACTTC TTCCCTGGTG GACACATCAG TCTCCACCAC TTCCCGGCCC 
CGCATGAAGA AGAAACTGAA GATGCTTAAC ATTGCCAAGA AGATCTTACG TATCCAAAAG 
GAGACTCCGA CCCTACAACG GAAGGAGCCC CCACCTTCAG TGCTGGAGGC TGACCTCACA 
GAGGGAGACC TGGCCAATTC CCACCTACCC TCTGAGGTAC TCTACATGCT TAAGAATGTT 
CGGGTGCTAG GTCACTTTGA GAAGCCGCTC TTCCTGGAGC TCTGCCGGCA CATGGTCTTC 
CAGCGTCTCG GGCAGGGGGA CTATGTTTTT CGGCCCGGTC AACCAGATGC CAGCATCTAT 
GTGGTTCAAG ATGGGCTGCT GGAGCTCTGC CTACCAGGGC CTGATGGGAA GGAGTGTGTG 
GTGAAGGAGG TGGTTCCTGG AGACAGCGTC AACAGCCTTC TGAGCATCCT TGATGTCATC 
ACGGGTCACC AGCATCCCCA GCGGACTGTG TCAGCCAGGG CTGCACGGGA CTCCACAGTG 
CTGAGACTCC CAGTAGAAGC CTTCTCTGCT GTCTTCACCA AGTACCCTGA GAGTTTGGTG 
CGAGTGGTAC AGATCATCAT GGTAAGGCTG CAGAGAGTCA CCTTCCTGGC GCTTCACAAT 
TACCTGGGTC TGACCAATGA ACTCTTCAGT CATGAGATCC AGCCCCTACG CCTTTTCCCC 
AGCCCCGGCC TCCCGACCCG AACCAGCCCT GTCCGTGGCT CTAAACGGGT AGTCAGCACC 
TCAGGTACTG AGGACACCTC AAAGGAGACC TCTGGCCGGC CCCTGGACTC CATTGGAGCT 
CCTCTGCCTG GACCTGCAGG GGATCCCGTG AAGCCCACAT CCTTAGAAGC ACCCCCAGCC 
CCGTTACTGA GCCGCTGCAT CTCCATGCCA GTAGACATCT CAGGCTTGCA AGGTGGCCCT 
CGTTCTGATT TTGACATGGC CTATGAACGT GGACGGATAT CTGTTTCTCT TCAAGAAGAG 
GCATCTGGGG GACCTCAGAC AGCATCCCCT AGGACCCCCA CTCAGGAGCT CCGGGAGCAG 
CCAGCGGGTG CCTGTGAATA TAGCTACTGT GAGGATGAGT CAGCCACAGG CGGATGTCCC 
TTTGGGCCCT ACCAGGGCCG CCAGACAAGC AGCATCTTTG AGGCTGCAAA GAGAGAGCTA 
GCCAAGCTGA TGCGGATTGA GGACCCCTCT CTACTGAACA GCAGAGTCTT GCTACATCAT 
GCCAAAGCCG GCACCATCAT AGCCCGCCAA GGGGACCAGG ATGTGAGCCT GCACTTTGTG 
CTCTGGGGCT GCCTGCACGT GTACCAGCGC ATGATTGACA AGGCTGAGGA AGTGTGCCTA 
TTTGTGGCAC AGCCAGGGGA GCTGGTGGGG CAACTGGCGG TGCTCACTGG GGAACCTCTC 
ATCTTCACAC TACGTGCCCA GAGAGACTGC ACCTTCCTGC GGATCTCCAA ATCCCACTTC 
TATGAGATCA TGCGTGCACA ACCCAGTGTG GTACTGAGTG CAGCTCACAC GGTGGCTGCT 
AGAATGTCCC CCTTCGTGCG CCAGATGGAC TTTGCCATTG ACTGGACAGC TGTGGAGGCT 
GGTCGTGCGC TCTACAGGCA GGGAGACCGC TCCGACTGCA CCTACATTGT ACTCAATGGA 
CGTCTCCGTA GTGTCATCCA ACGAGGCAGT GGCAAGAAGG AGCTAGTTGG GGAGTATGGT 
CGAGGGGATC TCATTGGCGT GGTGGAGGCA CTGACCCGGC AGCCACGAGC CACCACTGTA 
CACGCAGTGC GAGACACTGA GCTGGCCAAG CTTCCCGAGG GCACCTTAGG CCACATCAAA 
CGTCGGTACC CACAGGTTGT GACCCGCCTT ATTCATCTGC TAAGCCAGAA AATTCTAGGC 
AATCTGCAGC AGTTGCAAGG ACCCTTTCCA GGCTCCGGGC TCAGTGTTCC GCAGCACTCG 
GAACTGACCA ACCCAGCCAG CAATCTGTCT ACTGTAGCTA TCCTCCCTGT GTGTGCTGAG 
GTGCCCATGA TGGCCTTTAC TCTGGAACTG CAGCATGCTC TGCAAGCTAT TGGTCCCACG 
CTCCTCCTTA ACAGTGACGT CATCCGGGCA CTCCTGGGGG CTTCAGCACT GGATAGCATT 
CAAGAATTCC GGCTGTCAGG GTGGTTGGCC CAGCAGGAAG ATGCCCATCG CATTGTGCTC 
TACCAAACTG ACACATCCCT GACGCCTTGG ACCGTCCGTT GTCTGCGCCA GGCCGACTGC 
ATCCTCATTG TGGGCCTGGG GGACCAGGAA CCCACTGTTG GCCAGCTGGA GCAAATGTTA 
GAGAACACTG CTGTCCGTGC CTTGAAGCAA CTGGTTTTGC TGCACCGGGA GGAAGGCCCT 
GGTCCCACGC GCACTGTAGA GTGGCTCAAC ATGCGCAGCT GGTGCTCAGG GCACCTGCAC 
CTGCGCTGTC CTCGCCGCCT CTTCTCACGG CGCAGCCCAG CGAAACTTCA TGAGCTGTAT 
GAGAAGGTTT TCTCCAGGCG TGCAGACCGT CACAGCGACT TCTCCCGCTT GGCACGAGTA 
CTCACAGGAA ACACTATTGC TCTGGTGCTG GGTGGGGGCG GAGCCAGAGG CTGCTCGCAT 
ATTGGGGTGC TGAAGGCATT AGAGGAGGCA GGAGTCCCAG TCGACCTTGT GGGAGGCACA 
TCCATAGGTT CCTTCATTGG GGCCTTGTAT GCCGAGGAAC GCAGCGCCAG CCGAACTAAA 
CAACGAGCCC GGGAGTGGGC CAAGAGCATG ACTTCTGTAC TGGAGCCTGT ATTGGACCTC 
ACATATCCTG TCACCTCCAT GTTTACTGGC TCAGCCTTTA ACAGAAGTAT CCACCGTGTC 
TTCCAGGATA AGCAGATTGA GGACCTGTGG CTGCCTTACT TCAATGTGAC CACAGACATC 
ACTGCCTCTG CCATGCGCGT CCACAAAGAT GGCTCCCTGT GGCGGTATGT ACGTGCCAGC 
ATGACGCTCT CGGGCTACCT ACCCCCGCTG TGCGACCCAA AGGATGGGCA CCTGCTCATG 
GATGGTGGCT ACATCAACAA CCTGCCAGCG GATATTGCCC GAAGCATGGG AGCCAAAACG 
GTCATTGCCA TCGACGTTGG AAGCCAGGAT GAGACAGATC TCAGCACCTA TGGGGACAGC 
CTGTCTGGCT GGTGGTTGCT GTGGAAAAGG CTAAACCCCT GGGCAGACAA GGTAAAGGTT 
CCAGACATGG CTGAGATCCA GTCCCGCTTG GCGTACGTGT CCTGTGTGCG GCAGCTAGAA 
GTCGTCAAGT CCAGCTCCTA TTGCGAGTAC CTTCGTCCAT CCATTGACTG CTTCAAGACC 
ATGGACTTTG GAAAGTTTGA CCAGATCTAT GATGTGGGTT ACCAGTATGG GAAGGCTGTC 
TTTGGAGGCT GGACCCGTGG TGAAGTCATT GAGAAAATGC TCACAGACCG GAGATCTACA 
GACCTTAATG AGAGTCGCCG TGCAGATATA CTTGCCTTCC CAAGTTCTGG GTTCACTGAC 
TTGGCTGAGA TTGTGTCCCG GATCGAGCCA CCAACAAGCT ACGTCTCTGA TGGCTGTGCT 
GATGGGGAGG AGTCGGATTG CTTGACAGAG TATGAGGAAG ATGCAGGACC AGACTGCTCA 
AGAGATGAAG GGGGCTCCCC TGAGGGTGCC AGCCCTAGCA CTGCCTCAGA GGTGGAAGAA 
GAGAAGTCCA CACTCCGGCA ACGACGCTTT CTGCCTCAGG AGACTCCCAG CTCAGTCGCA 
GATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240368.1
CDS	238..4206
Translation	MRCPACAVRSAGAGRDDRGRSGGAGHRRAHPLGETPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_011242066.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242066.2

ORF Insert Sequence:

ATGCGTTGCC CAGCCTGTGC CGTTCGTTCC GCAGGTGCTG GGCGTGATGA TCGGGGCCGG 
AGTGGCGGTG CTGGTCACCG CCGTGCTCAT CCTCTTGGTG AAACTCCAGC CCCGGAGGGT 
CCCCGGTATC GGTTCCGGAA GCGGGACAAA GTGCTTTTCT ATGGCCGGAA GATTATGCGG 
AAGGTGTCAC AGTCCACTTC TTCCCTGGTG GACACATCAG TCTCCACCAC TTCCCGGCCC 
CGCATGAAGA AGAAACTGAA GATGCTTAAC ATTGCCAAGA AGATCTTACG TATCCAAAAG 
GAGACTCCGA CCCTACAACG GAAGGAGCCC CCACCTTCAG TGCTGGAGGC TGACCTCACA 
GAGGGAGACC TGGCCAATTC CCACCTACCC TCTGAGGTAC TCTACATGCT TAAGAATGTT 
CGGGTGCTAG GTCACTTTGA GAAGCCGCTC TTCCTGGAGC TCTGCCGGCA CATGGTCTTC 
CAGCGTCTCG GGCAGGGGGA CTATGTTTTT CGGCCCGGTC AACCAGATGC CAGCATCTAT 
GTGGTTCAAG ATGGGCTGCT GGAGCTCTGC CTACCAGGGC CTGATGGGAA GGAGTGTGTG 
GTGAAGGAGG TGGTTCCTGG AGACAGCGTC AACAGCCTTC TGAGCATCCT TGATGTCATC 
ACGGGTCACC AGCATCCCCA GCGGACTGTG TCAGCCAGGG CTGCACGGGA CTCCACAGTG 
CTGAGACTCC CAGTAGAAGC CTTCTCTGCT GTCTTCACCA AGTACCCTGA GAGTTTGGTG 
CGAGTGGTAC AGATCATCAT GGTAAGGCTG CAGAGAGTCA CCTTCCTGGC GCTTCACAAT 
TACCTGGGTC TGACCAATGA ACTCTTCAGT CATGAGATCC AGCCCCTACG CCTTTTCCCC 
AGCCCCGGCC TCCCGACCCG AACCAGCCCT GTCCGTGGCT CTAAACGGGT AGTCAGCACC 
TCAGGTACTG AGGACACCTC AAAGGAGACC TCTGGCCGGC CCCTGGACTC CATTGGAGCT 
CCTCTGCCTG GACCTGCAGG GGATCCCGTG AAGCCCACAT CCTTAGAAGC ACCCCCAGCC 
CCGTTACTGA GCCGCTGCAT CTCCATGCCA GTAGACATCT CAGGCTTGCA AGGTGGCCCT 
CGTTCTGATT TTGACATGGC CTATGAACGT GGACGGATAT CTGTTTCTCT TCAAGAAGAG 
GCATCTGGGG GACCTCAGAC AGCATCCCCT AGGACCCCCA CTCAGGAGCT CCGGGAGCAG 
CCAGCGGGTG CCTGTGAATA TAGCTACTGT GAGGATGAGT CAGCCACAGG CGGATGTCCC 
TTTGGGCCCT ACCAGGGCCG CCAGACAAGC AGCATCTTTG AGGCTGCAAA GAGAGAGCTA 
GCCAAGCTGA TGCGGATTGA GGACCCCTCT CTACTGAACA GCAGAGTCTT GCTACATCAT 
GCCAAAGCCG GCACCATCAT AGCCCGCCAA GGGGACCAGG ATGTGAGCCT GCACTTTGTG 
CTCTGGGGCT GCCTGCACGT GTACCAGCGC ATGATTGACA AGGCTGAGGA AGTGTGCCTA 
TTTGTGGCAC AGCCAGGGGA GCTGGTGGGG CAACTGGCGG TGCTCACTGG GGAACCTCTC 
ATCTTCACAC TACGTGCCCA GAGAGACTGC ACCTTCCTGC GGATCTCCAA ATCCCACTTC 
TATGAGATCA TGCGTGCACA ACCCAGTGTG GTACTGAGTG CAGCTCACAC GGTGGCTGCT 
AGAATGTCCC CCTTCGTGCG CCAGATGGAC TTTGCCATTG ACTGGACAGC TGTGGAGGCT 
GGTCGTGCGC TCTACAGGCA GGGAGACCGC TCCGACTGCA CCTACATTGT ACTCAATGGA 
CGTCTCCGTA GTGTCATCCA ACGAGGCAGT GGCAAGAAGG AGCTAGTTGG GGAGTATGGT 
CGAGGGGATC TCATTGGCGT GGTGGAGGCA CTGACCCGGC AGCCACGAGC CACCACTGTA 
CACGCAGTGC GAGACACTGA GCTGGCCAAG CTTCCCGAGG GCACCTTAGG CCACATCAAA 
CGTCGGTACC CACAGGTTGT GACCCGCCTT ATTCATCTGC TAAGCCAGAA AATTCTAGGC 
AATCTGCAGC AGTTGCAAGG ACCCTTTCCA GGCTCCGGGC TCAGTGTTCC GCAGCACTCG 
GAACTGACCA ACCCAGCCAG CAATCTGTCT ACTGTAGCTA TCCTCCCTGT GTGTGCTGAG 
GTGCCCATGA TGGCCTTTAC TCTGGAACTG CAGCATGCTC TGCAAGCTAT TGGTCCCACG 
CTCCTCCTTA ACAGTGACGT CATCCGGGCA CTCCTGGGGG CTTCAGCACT GGATAGCATT 
CAAGAATTCC GGCTGTCAGG GTGGTTGGCC CAGCAGGAAG ATGCCCATCG CATTGTGCTC 
TACCAAACTG ACACATCCCT GACGCCTTGG ACCGTCCGTT GTCTGCGCCA GGCCGACTGC 
ATCCTCATTG TGGGCCTGGG GGACCAGGAA CCCACTGTTG GCCAGCTGGA GCAAATGTTA 
GAGAACACTG CTGTCCGTGC CTTGAAGCAA CTGGTTTTGC TGCACCGGGA GGAAGGCCCT 
GGTCCCACGC GCACTGTAGA GTGGCTCAAC ATGCGCAGCT GGTGCTCAGG GCACCTGCAC 
CTGCGCTGTC CTCGCCGCCT CTTCTCACGG CGCAGCCCAG CGAAACTTCA TGAGCTGTAT 
GAGAAGGTTT TCTCCAGGCG TGCAGACCGT CACAGCGACT TCTCCCGCTT GGCACGAGTA 
CTCACAGGAA ACACTATTGC TCTGGTGCTG GGTGGGGGCG GAGCCAGAGG CTGCTCGCAT 
ATTGGGGTGC TGAAGGCATT AGAGGAGGCA GGAGTCCCAG TCGACCTTGT GGGAGGCACA 
TCCATAGGTT CCTTCATTGG GGCCTTGTAT GCCGAGGAAC GCAGCGCCAG CCGAACTAAA 
CAACGAGCCC GGGAGTGGGC CAAGAGCATG ACTTCTGTAC TGGAGCCTGT ATTGGACCTC 
ACATATCCTG TCACCTCCAT GTTTACTGGC TCAGCCTTTA ACAGAAGTAT CCACCGTGTC 
TTCCAGGATA AGCAGATTGA GGACCTGTGG CTGCCTTACT TCAATGTGAC CACAGACATC 
ACTGCCTCTG CCATGCGCGT CCACAAAGAT GGCTCCCTGT GGCGGTATGT ACGTGCCAGC 
ATGACGCTCT CGGGCTACCT ACCCCCGCTG TGCGACCCAA AGGATGGGCA CCTGCTCATG 
GATGGTGGCT ACATCAACAA CCTGCCAGCG GATATTGCCC GAAGCATGGG AGCCAAAACG 
GTCATTGCCA TCGACGTTGG AAGCCAGGAT GAGACAGATC TCAGCACCTA TGGGGACAGC 
CTGTCTGGCT GGTGGTTGCT GTGGAAAAGG CTAAACCCCT GGGCAGACAA GGTAAAGGTT 
CCAGACATGG CTGAGATCCA GTCCCGCTTG GCGTACGTGT CCTGTGTGCG GCAGCTAGAA 
GTCGTCAAGT CCAGCTCCTA TTGCGAGTAC CTTCGTCCAT CCATTGACTG CTTCAAGACC 
ATGGACTTTG GAAAGTTTGA CCAGATCTAT GATGTGGGTT ACCAGTATGG GAAGGCTGTC 
TTTGGAGGCT GGACCCGTGG TGAAGTCATT GAGAAAATGC TCACAGACCG GAGATCTACA 
GACCTTAATG AGAGTCGCCG TGCAGATATA CTTGCCTTCC CAAGTTCTGG GTTCACTGAC 
TTGGCTGAGA TTGTGTCCCG GATCGAGCCA CCAACAAGCT ACGTCTCTGA TGGCTGTGCT 
GATGGGGAGG AGTCGGATTG CTTGACAGAG TATGAGGAAG ATGCAGGACC AGACTGCTCA 
AGAGATGAAG GGGGCTCCCC TGAGGGTGCC AGCCCTAGCA CTGCCTCAGA GGTGGAAGAA 
GAGAAGTCCA CACTCCGGCA ACGACGCTTT CTGCCTCAGG AGACTCCCAG CTCAGTCGCA 
GATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( NM_001359123.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346052.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 5, mRNA.

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CloneID	OMu40422
Clone ID Related Accession (Same CDS sequence)	NM_001359123.1 , XM_006508822.3
Accession Version	NM_001359123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC170806.2. On Dec 21, 2017 this sequence version replaced XM_006508822.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.159228.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGGACAC CGAGTCACGA ACTGAATACA ACCTCCTCCG GGGCAGAGGT GATTCAAAAG 
ACCCTGGAGG AGGGCTTGGG GAGACGGATA TGCGTTGCCC AGCCTGTGCC GTTCGTTCCG 
CAGGTGCTGG GCGTGATGAT CGGGGCCGGA GTGGCGGTGC TGGTCACCGC CGTGCTCATC 
CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG AAAACTCCAG CCCCGGAGGG TCCCCGGTAT 
CGGTTCCGGA AGCGGGACAA AGTGCTTTTC TATGGCCGGA AGATTATGCG GAAGGTGTCA 
CAGTCCACTT CTTCCCTGGT GGACACATCA GTCTCCACCA CTTCCCGGCC CCGCATGAAG 
AAGAAACTGA AGATGCTTAA CATTGCCAAG AAGATCTTAC GTATCCAAAA GGAGACTCCG 
ACCCTACAAC GGAAGGAGCC CCCACCTTCA GTGCTGGAGG CTGACCTCAC AGAGGGAGAC 
CTGGCCAATT CCCACCTACC CTCTGAGGTA CTCTACATGC TTAAGAATGT TCGGGTGCTA 
GGTCACTTTG AGAAGCCGCT CTTCCTGGAG CTCTGCCGGC ACATGGTCTT CCAGCGTCTC 
GGGCAGGGGG ACTATGTTTT TCGGCCCGGT CAACCAGATG CCAGCATCTA TGTGGTTCAA 
GATGGGCTGC TGGAGCTCTG CCTACCAGGG CCTGATGGGA AGGAGTGTGT GGTGAAGGAG 
GTGGTTCCTG GAGACAGCGT CAACAGCCTT CTGAGCATCC TTGATGTCAT CACGGGTCAC 
CAGCATCCCC AGCGGACTGT GTCAGCCAGG GCTGCACGGG ACTCCACAGT GCTGAGACTC 
CCAGTAGAAG CCTTCTCTGC TGTCTTCACC AAGTACCCTG AGAGTTTGGT GCGAGTGGTA 
CAGATCATCA TGGTAAGGCT GCAGAGAGTC ACCTTCCTGG CGCTTCACAA TTACCTGGGT 
CTGACCAATG AACTCTTCAG TCATGAGATC CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC 
CTCCCGACCC GAACCAGCCC TGTCCGTGGC TCTAAACGGG TAGTCAGCAC CTCAGGTACT 
GAGGACACCT CAAAGGAGAC CTCTGGCCGG CCCCTGGACT CCATTGGAGC TCCTCTGCCT 
GGACCTGCAG GGGATCCCGT GAAGCCCACA TCCTTAGAAG CACCCCCAGC CCCGTTACTG 
AGCCGCTGCA TCTCCATGCC AGTAGACATC TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT 
TTTGACATGG CCTATGAACG TGGACGGATA TCTGTTTCTC TTCAAGAAGA GGCATCTGGG 
GGACCTCAGA CAGCATCCCC TAGGACCCCC ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT 
GCCTGTGAAT ATAGCTACTG TGAGGATGAG TCAGCCACAG GCGGATGTCC CTTTGGGCCC 
TACCAGGGCC GCCAGACAAG CAGCATCTTT GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG 
ATGCGGATTG AGGACCCCTC TCTACTGAAC AGCAGAGTCT TGCTACATCA TGCCAAAGCC 
GGCACCATCA TAGCCCGCCA AGGGGACCAG GATGTGAGCC TGCACTTTGT GCTCTGGGGC 
TGCCTGCACG TGTACCAGCG CATGATTGAC AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA 
CAGCCAGGGG AGCTGGTGGG GCAACTGGCG GTGCTCACTG GGGAACCTCT CATCTTCACA 
CTACGTGCCC AGAGAGACTG CACCTTCCTG CGGATCTCCA AATCCCACTT CTATGAGATC 
ATGCGTGCAC AACCCAGTGT GGTACTGAGT GCAGCTCACA CGGTGGCTGC TAGAATGTCC 
CCCTTCGTGC GCCAGATGGA CTTTGCCATT GACTGGACAG CTGTGGAGGC TGGTCGTGCG 
CTCTACAGGC AGGGAGACCG CTCCGACTGC ACCTACATTG TACTCAATGG ACGTCTCCGT 
AGTGTCATCC AACGAGGCAG TGGCAAGAAG GAGCTAGTTG GGGAGTATGG TCGAGGGGAT 
CTCATTGGCG TGGTGGAGGC ACTGACCCGG CAGCCACGAG CCACCACTGT ACACGCAGTG 
CGAGACACTG AGCTGGCCAA GCTTCCCGAG GGCACCTTAG GCCACATCAA ACGTCGGTAC 
CCACAGGTTG TGACCCGCCT TATTCATCTG CTAAGCCAGA AAATTCTAGG CAATCTGCAG 
CAGTTGCAAG GACCCTTTCC AGGCTCCGGG CTCAGTGTTC CGCAGCACTC GGAACTGACC 
AACCCAGCCA GCAATCTGTC TACTGTAGCT ATCCTCCCTG TGTGTGCTGA GGTGCCCATG 
ATGGCCTTTA CTCTGGAACT GCAGCATGCT CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT 
AACAGTGACG TCATCCGGGC ACTCCTGGGG GCTTCAGCAC TGGATAGCAT TCAAGAATTC 
CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA GATGCCCATC GCATTGTGCT CTACCAAACT 
GACACATCCC TGACGCCTTG GACCGTCCGT TGTCTGCGCC AGGCCGACTG CATCCTCATT 
GTGGGCCTGG GGGACCAGGA ACCCACTGTT GGCCAGCTGG AGCAAATGTT AGAGAACACT 
GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG CTGCACCGGG AGGAAGGCCC TGGTCCCACG 
CGCACTGTAG AGTGGCTCAA CATGCGCAGC TGGTGCTCAG GGCACCTGCA CCTGCGCTGT 
CCTCGCCGCC TCTTCTCACG GCGCAGCCCA GCGAAACTTC ATGAGCTGTA TGAGAAGGTT 
TTCTCCAGGC GTGCAGACCG TCACAGCGAC TTCTCCCGCT TGGCACGAGT ACTCACAGGA 
AACACTATTG CTCTGGTGCT GGGTGGGGGC GGAGCCAGAG GCTGCTCGCA TATTGGGGTG 
CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA GTCGACCTTG TGGGAGGCAC ATCCATAGGT 
TCCTTCATTG GGGCCTTGTA TGCCGAGGAA CGCAGCGCCA GCCGAACTAA ACAACGAGCC 
CGGGAGTGGG CCAAGAGCAT GACTTCTGTA CTGGAGCCTG TATTGGACCT CACATATCCT 
GTCACCTCCA TGTTTACTGG CTCAGCCTTT AACAGAAGTA TCCACCGTGT CTTCCAGGAT 
AAGCAGATTG AGGACCTGTG GCTGCCTTAC TTCAATGTGA CCACAGACAT CACTGCCTCT 
GCCATGCGCG TCCACAAAGA TGGCTCCCTG TGGCGGTATG TACGTGCCAG CATGACGCTC 
TCGGGCTACC TACCCCCGCT GTGCGACCCA AAGGATGGGC ACCTGCTCAT GGATGGTGGC 
TACATCAACA ACCTGCCAGC GGATATTGCC CGAAGCATGG GAGCCAAAAC GGTCATTGCC 
ATCGACGTTG GAAGCCAGGA TGAGACAGAT CTCAGCACCT ATGGGGACAG CCTGTCTGGC 
TGGTGGTTGC TGTGGAAAAG GCTAAACCCC TGGGCAGACA AGGTAAAGGT TCCAGACATG 
GCTGAGATCC AGTCCCGCTT GGCGTACGTG TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG 
TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA TCCATTGACT GCTTCAAGAC CATGGACTTT 
GGAAAGTTTG ACCAGATCTA TGATGTGGGT TACCAGTATG GGAAGGCTGT CTTTGGAGGC 
TGGACCCGTG GTGAAGTCAT TGAGAAAATG CTCACAGACC GGAGATCTAC AGACCTTAAT 
GAGAGTCGCC GTGCAGATAT ACTTGCCTTC CCAAGTTCTG GGTTCACTGA CTTGGCTGAG 
ATTGTGTCCC GGATCGAGCC ACCAACAAGC TACGTCTCTG ATGGCTGTGC TGATGGGGAG 
GAGTCGGATT GCTTGACAGA GTATGAGGAA GATGCAGGAC CAGACTGCTC AAGAGATGAA 
GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC 
ACACTCCGGC AACGACGCTT TCTGCCTCAG GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346052.1
CDS	92..4171
Translation	MGTPSHELNTTSSGAEVIQKTLEEGLGRRICVAQPVPFVPQVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	NM_001359123.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359123.1

ORF Insert Sequence:

ATGGGGACAC CGAGTCACGA ACTGAATACA ACCTCCTCCG GGGCAGAGGT GATTCAAAAG 
ACCCTGGAGG AGGGCTTGGG GAGACGGATA TGCGTTGCCC AGCCTGTGCC GTTCGTTCCG 
CAGGTGCTGG GCGTGATGAT CGGGGCCGGA GTGGCGGTGC TGGTCACCGC CGTGCTCATC 
CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG AAAACTCCAG CCCCGGAGGG TCCCCGGTAT 
CGGTTCCGGA AGCGGGACAA AGTGCTTTTC TATGGCCGGA AGATTATGCG GAAGGTGTCA 
CAGTCCACTT CTTCCCTGGT GGACACATCA GTCTCCACCA CTTCCCGGCC CCGCATGAAG 
AAGAAACTGA AGATGCTTAA CATTGCCAAG AAGATCTTAC GTATCCAAAA GGAGACTCCG 
ACCCTACAAC GGAAGGAGCC CCCACCTTCA GTGCTGGAGG CTGACCTCAC AGAGGGAGAC 
CTGGCCAATT CCCACCTACC CTCTGAGGTA CTCTACATGC TTAAGAATGT TCGGGTGCTA 
GGTCACTTTG AGAAGCCGCT CTTCCTGGAG CTCTGCCGGC ACATGGTCTT CCAGCGTCTC 
GGGCAGGGGG ACTATGTTTT TCGGCCCGGT CAACCAGATG CCAGCATCTA TGTGGTTCAA 
GATGGGCTGC TGGAGCTCTG CCTACCAGGG CCTGATGGGA AGGAGTGTGT GGTGAAGGAG 
GTGGTTCCTG GAGACAGCGT CAACAGCCTT CTGAGCATCC TTGATGTCAT CACGGGTCAC 
CAGCATCCCC AGCGGACTGT GTCAGCCAGG GCTGCACGGG ACTCCACAGT GCTGAGACTC 
CCAGTAGAAG CCTTCTCTGC TGTCTTCACC AAGTACCCTG AGAGTTTGGT GCGAGTGGTA 
CAGATCATCA TGGTAAGGCT GCAGAGAGTC ACCTTCCTGG CGCTTCACAA TTACCTGGGT 
CTGACCAATG AACTCTTCAG TCATGAGATC CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC 
CTCCCGACCC GAACCAGCCC TGTCCGTGGC TCTAAACGGG TAGTCAGCAC CTCAGGTACT 
GAGGACACCT CAAAGGAGAC CTCTGGCCGG CCCCTGGACT CCATTGGAGC TCCTCTGCCT 
GGACCTGCAG GGGATCCCGT GAAGCCCACA TCCTTAGAAG CACCCCCAGC CCCGTTACTG 
AGCCGCTGCA TCTCCATGCC AGTAGACATC TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT 
TTTGACATGG CCTATGAACG TGGACGGATA TCTGTTTCTC TTCAAGAAGA GGCATCTGGG 
GGACCTCAGA CAGCATCCCC TAGGACCCCC ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT 
GCCTGTGAAT ATAGCTACTG TGAGGATGAG TCAGCCACAG GCGGATGTCC CTTTGGGCCC 
TACCAGGGCC GCCAGACAAG CAGCATCTTT GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG 
ATGCGGATTG AGGACCCCTC TCTACTGAAC AGCAGAGTCT TGCTACATCA TGCCAAAGCC 
GGCACCATCA TAGCCCGCCA AGGGGACCAG GATGTGAGCC TGCACTTTGT GCTCTGGGGC 
TGCCTGCACG TGTACCAGCG CATGATTGAC AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA 
CAGCCAGGGG AGCTGGTGGG GCAACTGGCG GTGCTCACTG GGGAACCTCT CATCTTCACA 
CTACGTGCCC AGAGAGACTG CACCTTCCTG CGGATCTCCA AATCCCACTT CTATGAGATC 
ATGCGTGCAC AACCCAGTGT GGTACTGAGT GCAGCTCACA CGGTGGCTGC TAGAATGTCC 
CCCTTCGTGC GCCAGATGGA CTTTGCCATT GACTGGACAG CTGTGGAGGC TGGTCGTGCG 
CTCTACAGGC AGGGAGACCG CTCCGACTGC ACCTACATTG TACTCAATGG ACGTCTCCGT 
AGTGTCATCC AACGAGGCAG TGGCAAGAAG GAGCTAGTTG GGGAGTATGG TCGAGGGGAT 
CTCATTGGCG TGGTGGAGGC ACTGACCCGG CAGCCACGAG CCACCACTGT ACACGCAGTG 
CGAGACACTG AGCTGGCCAA GCTTCCCGAG GGCACCTTAG GCCACATCAA ACGTCGGTAC 
CCACAGGTTG TGACCCGCCT TATTCATCTG CTAAGCCAGA AAATTCTAGG CAATCTGCAG 
CAGTTGCAAG GACCCTTTCC AGGCTCCGGG CTCAGTGTTC CGCAGCACTC GGAACTGACC 
AACCCAGCCA GCAATCTGTC TACTGTAGCT ATCCTCCCTG TGTGTGCTGA GGTGCCCATG 
ATGGCCTTTA CTCTGGAACT GCAGCATGCT CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT 
AACAGTGACG TCATCCGGGC ACTCCTGGGG GCTTCAGCAC TGGATAGCAT TCAAGAATTC 
CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA GATGCCCATC GCATTGTGCT CTACCAAACT 
GACACATCCC TGACGCCTTG GACCGTCCGT TGTCTGCGCC AGGCCGACTG CATCCTCATT 
GTGGGCCTGG GGGACCAGGA ACCCACTGTT GGCCAGCTGG AGCAAATGTT AGAGAACACT 
GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG CTGCACCGGG AGGAAGGCCC TGGTCCCACG 
CGCACTGTAG AGTGGCTCAA CATGCGCAGC TGGTGCTCAG GGCACCTGCA CCTGCGCTGT 
CCTCGCCGCC TCTTCTCACG GCGCAGCCCA GCGAAACTTC ATGAGCTGTA TGAGAAGGTT 
TTCTCCAGGC GTGCAGACCG TCACAGCGAC TTCTCCCGCT TGGCACGAGT ACTCACAGGA 
AACACTATTG CTCTGGTGCT GGGTGGGGGC GGAGCCAGAG GCTGCTCGCA TATTGGGGTG 
CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA GTCGACCTTG TGGGAGGCAC ATCCATAGGT 
TCCTTCATTG GGGCCTTGTA TGCCGAGGAA CGCAGCGCCA GCCGAACTAA ACAACGAGCC 
CGGGAGTGGG CCAAGAGCAT GACTTCTGTA CTGGAGCCTG TATTGGACCT CACATATCCT 
GTCACCTCCA TGTTTACTGG CTCAGCCTTT AACAGAAGTA TCCACCGTGT CTTCCAGGAT 
AAGCAGATTG AGGACCTGTG GCTGCCTTAC TTCAATGTGA CCACAGACAT CACTGCCTCT 
GCCATGCGCG TCCACAAAGA TGGCTCCCTG TGGCGGTATG TACGTGCCAG CATGACGCTC 
TCGGGCTACC TACCCCCGCT GTGCGACCCA AAGGATGGGC ACCTGCTCAT GGATGGTGGC 
TACATCAACA ACCTGCCAGC GGATATTGCC CGAAGCATGG GAGCCAAAAC GGTCATTGCC 
ATCGACGTTG GAAGCCAGGA TGAGACAGAT CTCAGCACCT ATGGGGACAG CCTGTCTGGC 
TGGTGGTTGC TGTGGAAAAG GCTAAACCCC TGGGCAGACA AGGTAAAGGT TCCAGACATG 
GCTGAGATCC AGTCCCGCTT GGCGTACGTG TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG 
TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA TCCATTGACT GCTTCAAGAC CATGGACTTT 
GGAAAGTTTG ACCAGATCTA TGATGTGGGT TACCAGTATG GGAAGGCTGT CTTTGGAGGC 
TGGACCCGTG GTGAAGTCAT TGAGAAAATG CTCACAGACC GGAGATCTAC AGACCTTAAT 
GAGAGTCGCC GTGCAGATAT ACTTGCCTTC CCAAGTTCTG GGTTCACTGA CTTGGCTGAG 
ATTGTGTCCC GGATCGAGCC ACCAACAAGC TACGTCTCTG ATGGCTGTGC TGATGGGGAG 
GAGTCGGATT GCTTGACAGA GTATGAGGAA GATGCAGGAC CAGACTGCTC AAGAGATGAA 
GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC 
ACACTCCGGC AACGACGCTT TCTGCCTCAG GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( NM_001359124.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346053.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 6, mRNA.

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CloneID	OMu40423
Clone ID Related Accession (Same CDS sequence)	XM_006508823.3 , NM_001359124.1
Accession Version	NM_001359124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC170806.2. On Dec 21, 2017 this sequence version replaced XM_006508823.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.157043.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGGACAC CGAGTCACGA ACTGAATACA ACCTCCTCCG GGGCAGAGGT GATTCAAAAG 
ACCCTGGAGG AGGGCTTGGG GAGACGGATA TGCGTTGCCC AGCCTGTGCC GTTCGTTCCG 
CAGGTGCTGG GCGTGATGAT CGGGGCCGGA GTGGCGGTGC TGGTCACCGC CGTGCTCATC 
CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG AAAACTCCAG CCCCGGAGGG TCCCCGGTAT 
CGGTTCCGGA AGCGGGACAA AGTGCTTTTC TATGGCCGGA AGATTATGCG GAAGGTGTCA 
CAGTCCACTT CTTCCCTGGT GGACACATCA GTCTCCACCA CTTCCCGGCC CCGCATGAAG 
AAGAAACTGA AGATGCTTAA CATTGCCAAG AAGATCTTAC GTATCCAAAA GGAGACTCCG 
ACCCTACAAC GGAAGGAGCC CCCACCTTCA GTGCTGGAGG CTGACCTCAC AGAGGGAGAC 
CTGGCCAATT CCCACCTACC CTCTGAGGTA CTCTACATGC TTAAGAATGT TCGGGTGCTA 
GGTCACTTTG AGAAGCCGCT CTTCCTGGAG CTCTGCCGGC ACATGGTCTT CCAGCGTCTC 
GGGCAGGGGG ACTATGTTTT TCGGCCCGGT CAACCAGATG CCAGCATCTA TGTGGTTCAA 
GATGGGCTGC TGGAGCTCTG CCTACCAGGG CCTGATGGGA AGGAGTGTGT GGTGAAGGAG 
GTGGTTCCTG GAGACAGCGT CAACAGCCTT CTGAGCATCC TTGATGTCAT CACGGGTCAC 
CAGCATCCCC AGCGGACTGT GTCAGCCAGG GCTGCACGGG ACTCCACAGT GCTGAGACTC 
CCAGTAGAAG CCTTCTCTGC TGTCTTCACC AAGTACCCTG AGAGTTTGGT GCGAGTGGTA 
CAGATCATCA TGGTAAGGCT GCAGAGAGTC ACCTTCCTGG CGCTTCACAA TTACCTGGGT 
CTGACCAATG AACTCTTCAG TCATGAGATC CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC 
CTCCCGACCC GAACCAGCCC TGTCCGTGGC TCTAAACGGG TAGTCAGCAC CTCAGGTACT 
GAGGACACCT CAAAGGAGAC CTCTGGCCGG CCCCTGGACT CCATTGGAGC TCCTCTGCCT 
GGACCTGCAG GGGATCCCGT GAAGCCCACA TCCTTAGAAG CACCCCCAGC CCCGTTACTG 
AGCCGCTGCA TCTCCATGCC AGTAGACATC TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT 
TTTGACATGG CCTATGAACG TGGACGGATA TCTGTTTCTC TTCAAGAAGA GGCATCTGGG 
GGACCTCAGA CAGCATCCCC TAGGACCCCC ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT 
GCCTGTGAAT ATAGCTACTG TGAGGATGAG TCAGCCACAG GCGGATGTCC CTTTGGGCCC 
TACCAGGGCC GCCAGACAAG CAGCATCTTT GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG 
ATGCGGATTG AGGACCCCTC TCTACTGAAC AGCAGAGTCT TGCTACATCA TGCCAAAGCC 
GGCACCATCA TAGCCCGCCA AGGGGACCAG GATGTGAGCC TGCACTTTGT GCTCTGGGGC 
TGCCTGCACG TGTACCAGCG CATGATTGAC AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA 
CAGCCAGGGG AGCTGGTGGG GCAACTGGCG GTGCTCACTG GGGAACCTCT CATCTTCACA 
CTACGTGCCC AGAGAGACTG CACCTTCCTG CGGATCTCCA AATCCCACTT CTATGAGATC 
ATGCGTGCAC AACCCAGTGT GGTACTGAGT GCAGCTCACA CGGTGGCTGC TAGAATGTCC 
CCCTTCGTGC GCCAGATGGA CTTTGCCATT GACTGGACAG CTGTGGAGGC TGGTCGTGCG 
CTCTACAGGC AGGGAGACCG CTCCGACTGC ACCTACATTG TACTCAATGG ACGTCTCCGT 
AGTGTCATCC AACGAGGCAG TGGCAAGAAG GAGCTAGTTG GGGAGTATGG TCGAGGGGAT 
CTCATTGGCG TGGTGGAGGC ACTGACCCGG CAGCCACGAG CCACCACTGT ACACGCAGTG 
CGAGACACTG AGCTGGCCAA GCTTCCCGAG GGCACCTTAG GCCACATCAA ACGTCGGTAC 
CCACAGGTTG TGACCCGCCT TATTCATCTG CTAAGCCAGA AAATTCTAGG CAATCTGCAG 
CAGTTGCAAG GACCCTTTCC AGGCTCCGGG CTCAGTGTTC CGCAGCACTC GGAACTGACC 
AACCCAGCCA GCAATCTGTC TACTGTAGCT ATCCTCCCTG TGTGTGCTGA GGTGCCCATG 
ATGGCCTTTA CTCTGGAACT GCAGCATGCT CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT 
AACAGTGACG TCATCCGGGC ACTCCTGGGG GCTTCAGCAC TGGATAGCAT TCAAGAATTC 
CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA GATGCCCATC GCATTGTGCT CTACCAAACT 
GACACATCCC TGACGCCTTG GACCGTCCGT TGTCTGCGCC AGGCCGACTG CATCCTCATT 
GTGGGCCTGG GGGACCAGGA ACCCACTGTT GGCCAGCTGG AGCAAATGTT AGAGAACACT 
GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG CTGCACCGGG AGGAAGGCCC TGGTCCCACG 
CGCACTGTAG AGTGGCTCAA CATGCGCAGC TGGTGCTCAG GGCACCTGCA CCTGCGCTGT 
CCTCGCCGCC TCTTCTCACG GCGCAGCCCA GCGAAACTTC ATGAGCTGTA TGAGAAGGTT 
TTCTCCAGGC GTGCAGACCG TCACAGCGAC TTCTCCCGCT TGGCACGAGT ACTCACAGGA 
AACACTATTG CTCTGGTGCT GGGTGGGGGC GGAGCCAGAG GCTGCTCGCA TATTGGGGTG 
CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA GTCGACCTTG TGGGAGGCAC ATCCATAGGT 
TCCTTCATTG GGGCCTTGTA TGCCGAGGAA CGCAGCGCCA GCCGAACTAA ACAACGAGCC 
CGGGAGTGGG CCAAGAGCAT GACTTCTGTA CTGGAGCCTG TATTGGACCT CACATATCCT 
GTCACCTCCA TGTTTACTGG CTCAGCCTTT AACAGAAGTA TCCACCGTGT CTTCCAGGAT 
AAGCAGATTG AGGACCTGTG GCTGCCTTAC TTCAATGTGA CCACAGACAT CACTGCCTCT 
GCCATGCGCG TCCACAAAGA TGGCTGCGTG TGGCGTTACG TCCGGGCCAG TGCCTCCTAC 
TGTCCCTACC TGCCCCCACT CTGCGACCCC AAGGACGGGC ACCTGCTGGT GGACGGGTGT 
TACGTTAACA ACGTTCCAGC GGATATTGCC CGAAGCATGG GAGCCAAAAC GGTCATTGCC 
ATCGACGTTG GAAGCCAGGA TGAGACAGAT CTCAGCACCT ATGGGGACAG CCTGTCTGGC 
TGGTGGTTGC TGTGGAAAAG GCTAAACCCC TGGGCAGACA AGGTAAAGGT TCCAGACATG 
GCTGAGATCC AGTCCCGCTT GGCGTACGTG TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG 
TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA TCCATTGACT GCTTCAAGAC CATGGACTTT 
GGAAAGTTTG ACCAGATCTA TGATGTGGGT TACCAGTATG GGAAGGCTGT CTTTGGAGGC 
TGGACCCGTG GTGAAGTCAT TGAGAAAATG CTCACAGACC GGAGATCTAC AGACCTTAAT 
GAGAGTCGCC GTGCAGATAT ACTTGCCTTC CCAAGTTCTG GGTTCACTGA CTTGGCTGAG 
ATTGTGTCCC GGATCGAGCC ACCAACAAGC TACGTCTCTG ATGGCTGTGC TGATGGGGAG 
GAGTCGGATT GCTTGACAGA GTATGAGGAA GATGCAGGAC CAGACTGCTC AAGAGATGAA 
GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC 
ACACTCCGGC AACGACGCTT TCTGCCTCAG GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346053.1
CDS	92..4171
Translation	MGTPSHELNTTSSGAEVIQKTLEEGLGRRICVAQPVPFVPQVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGCVWRYVRASASYCPYLPPLCDPKDGHLLVDGCYVNNVPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	NM_001359124.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359124.1

ORF Insert Sequence:

ATGGGGACAC CGAGTCACGA ACTGAATACA ACCTCCTCCG GGGCAGAGGT GATTCAAAAG 
ACCCTGGAGG AGGGCTTGGG GAGACGGATA TGCGTTGCCC AGCCTGTGCC GTTCGTTCCG 
CAGGTGCTGG GCGTGATGAT CGGGGCCGGA GTGGCGGTGC TGGTCACCGC CGTGCTCATC 
CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG AAAACTCCAG CCCCGGAGGG TCCCCGGTAT 
CGGTTCCGGA AGCGGGACAA AGTGCTTTTC TATGGCCGGA AGATTATGCG GAAGGTGTCA 
CAGTCCACTT CTTCCCTGGT GGACACATCA GTCTCCACCA CTTCCCGGCC CCGCATGAAG 
AAGAAACTGA AGATGCTTAA CATTGCCAAG AAGATCTTAC GTATCCAAAA GGAGACTCCG 
ACCCTACAAC GGAAGGAGCC CCCACCTTCA GTGCTGGAGG CTGACCTCAC AGAGGGAGAC 
CTGGCCAATT CCCACCTACC CTCTGAGGTA CTCTACATGC TTAAGAATGT TCGGGTGCTA 
GGTCACTTTG AGAAGCCGCT CTTCCTGGAG CTCTGCCGGC ACATGGTCTT CCAGCGTCTC 
GGGCAGGGGG ACTATGTTTT TCGGCCCGGT CAACCAGATG CCAGCATCTA TGTGGTTCAA 
GATGGGCTGC TGGAGCTCTG CCTACCAGGG CCTGATGGGA AGGAGTGTGT GGTGAAGGAG 
GTGGTTCCTG GAGACAGCGT CAACAGCCTT CTGAGCATCC TTGATGTCAT CACGGGTCAC 
CAGCATCCCC AGCGGACTGT GTCAGCCAGG GCTGCACGGG ACTCCACAGT GCTGAGACTC 
CCAGTAGAAG CCTTCTCTGC TGTCTTCACC AAGTACCCTG AGAGTTTGGT GCGAGTGGTA 
CAGATCATCA TGGTAAGGCT GCAGAGAGTC ACCTTCCTGG CGCTTCACAA TTACCTGGGT 
CTGACCAATG AACTCTTCAG TCATGAGATC CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC 
CTCCCGACCC GAACCAGCCC TGTCCGTGGC TCTAAACGGG TAGTCAGCAC CTCAGGTACT 
GAGGACACCT CAAAGGAGAC CTCTGGCCGG CCCCTGGACT CCATTGGAGC TCCTCTGCCT 
GGACCTGCAG GGGATCCCGT GAAGCCCACA TCCTTAGAAG CACCCCCAGC CCCGTTACTG 
AGCCGCTGCA TCTCCATGCC AGTAGACATC TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT 
TTTGACATGG CCTATGAACG TGGACGGATA TCTGTTTCTC TTCAAGAAGA GGCATCTGGG 
GGACCTCAGA CAGCATCCCC TAGGACCCCC ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT 
GCCTGTGAAT ATAGCTACTG TGAGGATGAG TCAGCCACAG GCGGATGTCC CTTTGGGCCC 
TACCAGGGCC GCCAGACAAG CAGCATCTTT GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG 
ATGCGGATTG AGGACCCCTC TCTACTGAAC AGCAGAGTCT TGCTACATCA TGCCAAAGCC 
GGCACCATCA TAGCCCGCCA AGGGGACCAG GATGTGAGCC TGCACTTTGT GCTCTGGGGC 
TGCCTGCACG TGTACCAGCG CATGATTGAC AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA 
CAGCCAGGGG AGCTGGTGGG GCAACTGGCG GTGCTCACTG GGGAACCTCT CATCTTCACA 
CTACGTGCCC AGAGAGACTG CACCTTCCTG CGGATCTCCA AATCCCACTT CTATGAGATC 
ATGCGTGCAC AACCCAGTGT GGTACTGAGT GCAGCTCACA CGGTGGCTGC TAGAATGTCC 
CCCTTCGTGC GCCAGATGGA CTTTGCCATT GACTGGACAG CTGTGGAGGC TGGTCGTGCG 
CTCTACAGGC AGGGAGACCG CTCCGACTGC ACCTACATTG TACTCAATGG ACGTCTCCGT 
AGTGTCATCC AACGAGGCAG TGGCAAGAAG GAGCTAGTTG GGGAGTATGG TCGAGGGGAT 
CTCATTGGCG TGGTGGAGGC ACTGACCCGG CAGCCACGAG CCACCACTGT ACACGCAGTG 
CGAGACACTG AGCTGGCCAA GCTTCCCGAG GGCACCTTAG GCCACATCAA ACGTCGGTAC 
CCACAGGTTG TGACCCGCCT TATTCATCTG CTAAGCCAGA AAATTCTAGG CAATCTGCAG 
CAGTTGCAAG GACCCTTTCC AGGCTCCGGG CTCAGTGTTC CGCAGCACTC GGAACTGACC 
AACCCAGCCA GCAATCTGTC TACTGTAGCT ATCCTCCCTG TGTGTGCTGA GGTGCCCATG 
ATGGCCTTTA CTCTGGAACT GCAGCATGCT CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT 
AACAGTGACG TCATCCGGGC ACTCCTGGGG GCTTCAGCAC TGGATAGCAT TCAAGAATTC 
CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA GATGCCCATC GCATTGTGCT CTACCAAACT 
GACACATCCC TGACGCCTTG GACCGTCCGT TGTCTGCGCC AGGCCGACTG CATCCTCATT 
GTGGGCCTGG GGGACCAGGA ACCCACTGTT GGCCAGCTGG AGCAAATGTT AGAGAACACT 
GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG CTGCACCGGG AGGAAGGCCC TGGTCCCACG 
CGCACTGTAG AGTGGCTCAA CATGCGCAGC TGGTGCTCAG GGCACCTGCA CCTGCGCTGT 
CCTCGCCGCC TCTTCTCACG GCGCAGCCCA GCGAAACTTC ATGAGCTGTA TGAGAAGGTT 
TTCTCCAGGC GTGCAGACCG TCACAGCGAC TTCTCCCGCT TGGCACGAGT ACTCACAGGA 
AACACTATTG CTCTGGTGCT GGGTGGGGGC GGAGCCAGAG GCTGCTCGCA TATTGGGGTG 
CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA GTCGACCTTG TGGGAGGCAC ATCCATAGGT 
TCCTTCATTG GGGCCTTGTA TGCCGAGGAA CGCAGCGCCA GCCGAACTAA ACAACGAGCC 
CGGGAGTGGG CCAAGAGCAT GACTTCTGTA CTGGAGCCTG TATTGGACCT CACATATCCT 
GTCACCTCCA TGTTTACTGG CTCAGCCTTT AACAGAAGTA TCCACCGTGT CTTCCAGGAT 
AAGCAGATTG AGGACCTGTG GCTGCCTTAC TTCAATGTGA CCACAGACAT CACTGCCTCT 
GCCATGCGCG TCCACAAAGA TGGCTGCGTG TGGCGTTACG TCCGGGCCAG TGCCTCCTAC 
TGTCCCTACC TGCCCCCACT CTGCGACCCC AAGGACGGGC ACCTGCTGGT GGACGGGTGT 
TACGTTAACA ACGTTCCAGC GGATATTGCC CGAAGCATGG GAGCCAAAAC GGTCATTGCC 
ATCGACGTTG GAAGCCAGGA TGAGACAGAT CTCAGCACCT ATGGGGACAG CCTGTCTGGC 
TGGTGGTTGC TGTGGAAAAG GCTAAACCCC TGGGCAGACA AGGTAAAGGT TCCAGACATG 
GCTGAGATCC AGTCCCGCTT GGCGTACGTG TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG 
TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA TCCATTGACT GCTTCAAGAC CATGGACTTT 
GGAAAGTTTG ACCAGATCTA TGATGTGGGT TACCAGTATG GGAAGGCTGT CTTTGGAGGC 
TGGACCCGTG GTGAAGTCAT TGAGAAAATG CTCACAGACC GGAGATCTAC AGACCTTAAT 
GAGAGTCGCC GTGCAGATAT ACTTGCCTTC CCAAGTTCTG GGTTCACTGA CTTGGCTGAG 
ATTGTGTCCC GGATCGAGCC ACCAACAAGC TACGTCTCTG ATGGCTGTGC TGATGGGGAG 
GAGTCGGATT GCTTGACAGA GTATGAGGAA GATGCAGGAC CAGACTGCTC AAGAGATGAA 
GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC 
ACACTCCGGC AACGACGCTT TCTGCCTCAG GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( NM_001359122.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346051.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 4, mRNA.

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CloneID	OMu40426
Clone ID Related Accession (Same CDS sequence)	XM_006508826.3 , XM_006508829.1 , XM_006508827.4 , XM_006508829.2 , XM_006508827.3 , NM_001359122.1 , XM_006508828.1
Accession Version	NM_001359122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC170806.2. On Dec 21, 2017 this sequence version replaced XM_006508826.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.236105.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGACCCCC 
ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG 
TCAGCCACAG GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT 
GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC 
AGCAGAGTCT TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG 
GATGTGAGCC TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC 
AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG 
GTGCTCACTG GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG 
CGGATCTCCA AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT 
GCAGCTCACA CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT 
GACTGGACAG CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC 
ACCTACATTG TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG 
GAGCTAGTTG GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG 
CAGCCACGAG CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG 
GGCACCTTAG GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG 
CTAAGCCAGA AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG 
CTCAGTGTTC CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT 
ATCCTCCCTG TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT 
CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG 
GCTTCAGCAC TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA 
GATGCCCATC GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT 
TGTCTGCGCC AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT 
GGCCAGCTGG AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG 
CTGCACCGGG AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC 
TGGTGCTCAG GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA 
GCGAAACTTC ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC 
TTCTCCCGCT TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC 
GGAGCCAGAG GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA 
GTCGACCTTG TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA 
CGCAGCGCCA GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA 
CTGGAGCCTG TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT 
AACAGAAGTA TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC 
TTCAATGTGA CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG 
TGGCGGTATG TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA 
AAGGATGGGC ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC 
CGAAGCATGG GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT 
CTCAGCACCT ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC 
TGGGCAGACA AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG 
TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA 
TCCATTGACT GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT 
TACCAGTATG GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG 
CTCACAGACC GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC 
CCAAGTTCTG GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC 
TACGTCTCTG ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA 
GATGCAGGAC CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC 
ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG 
GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346051.1
CDS	212..4261
Translation	MEESLQPKDCRLQDLLPSPKQSMEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	NM_001359122.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359122.1

ORF Insert Sequence:

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGACCCCC 
ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG 
TCAGCCACAG GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT 
GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC 
AGCAGAGTCT TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG 
GATGTGAGCC TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC 
AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG 
GTGCTCACTG GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG 
CGGATCTCCA AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT 
GCAGCTCACA CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT 
GACTGGACAG CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC 
ACCTACATTG TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG 
GAGCTAGTTG GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG 
CAGCCACGAG CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG 
GGCACCTTAG GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG 
CTAAGCCAGA AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG 
CTCAGTGTTC CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT 
ATCCTCCCTG TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT 
CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG 
GCTTCAGCAC TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA 
GATGCCCATC GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT 
TGTCTGCGCC AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT 
GGCCAGCTGG AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG 
CTGCACCGGG AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC 
TGGTGCTCAG GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA 
GCGAAACTTC ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC 
TTCTCCCGCT TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC 
GGAGCCAGAG GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA 
GTCGACCTTG TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA 
CGCAGCGCCA GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA 
CTGGAGCCTG TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT 
AACAGAAGTA TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC 
TTCAATGTGA CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG 
TGGCGGTATG TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA 
AAGGATGGGC ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC 
CGAAGCATGG GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT 
CTCAGCACCT ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC 
TGGGCAGACA AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG 
TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA 
TCCATTGACT GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT 
TACCAGTATG GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG 
CTCACAGACC GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC 
CCAAGTTCTG GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC 
TACGTCTCTG ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA 
GATGCAGGAC CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC 
ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG 
GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508822.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508885.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X1, mRNA.

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CloneID	OMu40422
Clone ID Related Accession (Same CDS sequence)	NM_001359123.1 , XM_006508822.3
Accession Version	XM_006508822.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	neuropathy target esterase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508822.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGACAC CGAGTCACGA ACTGAATACA ACCTCCTCCG GGGCAGAGGT GATTCAAAAG 
ACCCTGGAGG AGGGCTTGGG GAGACGGATA TGCGTTGCCC AGCCTGTGCC GTTCGTTCCG 
CAGGTGCTGG GCGTGATGAT CGGGGCCGGA GTGGCGGTGC TGGTCACCGC CGTGCTCATC 
CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG AAAACTCCAG CCCCGGAGGG TCCCCGGTAT 
CGGTTCCGGA AGCGGGACAA AGTGCTTTTC TATGGCCGGA AGATTATGCG GAAGGTGTCA 
CAGTCCACTT CTTCCCTGGT GGACACATCA GTCTCCACCA CTTCCCGGCC CCGCATGAAG 
AAGAAACTGA AGATGCTTAA CATTGCCAAG AAGATCTTAC GTATCCAAAA GGAGACTCCG 
ACCCTACAAC GGAAGGAGCC CCCACCTTCA GTGCTGGAGG CTGACCTCAC AGAGGGAGAC 
CTGGCCAATT CCCACCTACC CTCTGAGGTA CTCTACATGC TTAAGAATGT TCGGGTGCTA 
GGTCACTTTG AGAAGCCGCT CTTCCTGGAG CTCTGCCGGC ACATGGTCTT CCAGCGTCTC 
GGGCAGGGGG ACTATGTTTT TCGGCCCGGT CAACCAGATG CCAGCATCTA TGTGGTTCAA 
GATGGGCTGC TGGAGCTCTG CCTACCAGGG CCTGATGGGA AGGAGTGTGT GGTGAAGGAG 
GTGGTTCCTG GAGACAGCGT CAACAGCCTT CTGAGCATCC TTGATGTCAT CACGGGTCAC 
CAGCATCCCC AGCGGACTGT GTCAGCCAGG GCTGCACGGG ACTCCACAGT GCTGAGACTC 
CCAGTAGAAG CCTTCTCTGC TGTCTTCACC AAGTACCCTG AGAGTTTGGT GCGAGTGGTA 
CAGATCATCA TGGTAAGGCT GCAGAGAGTC ACCTTCCTGG CGCTTCACAA TTACCTGGGT 
CTGACCAATG AACTCTTCAG TCATGAGATC CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC 
CTCCCGACCC GAACCAGCCC TGTCCGTGGC TCTAAACGGG TAGTCAGCAC CTCAGGTACT 
GAGGACACCT CAAAGGAGAC CTCTGGCCGG CCCCTGGACT CCATTGGAGC TCCTCTGCCT 
GGACCTGCAG GGGATCCCGT GAAGCCCACA TCCTTAGAAG CACCCCCAGC CCCGTTACTG 
AGCCGCTGCA TCTCCATGCC AGTAGACATC TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT 
TTTGACATGG CCTATGAACG TGGACGGATA TCTGTTTCTC TTCAAGAAGA GGCATCTGGG 
GGACCTCAGA CAGCATCCCC TAGGACCCCC ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT 
GCCTGTGAAT ATAGCTACTG TGAGGATGAG TCAGCCACAG GCGGATGTCC CTTTGGGCCC 
TACCAGGGCC GCCAGACAAG CAGCATCTTT GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG 
ATGCGGATTG AGGACCCCTC TCTACTGAAC AGCAGAGTCT TGCTACATCA TGCCAAAGCC 
GGCACCATCA TAGCCCGCCA AGGGGACCAG GATGTGAGCC TGCACTTTGT GCTCTGGGGC 
TGCCTGCACG TGTACCAGCG CATGATTGAC AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA 
CAGCCAGGGG AGCTGGTGGG GCAACTGGCG GTGCTCACTG GGGAACCTCT CATCTTCACA 
CTACGTGCCC AGAGAGACTG CACCTTCCTG CGGATCTCCA AATCCCACTT CTATGAGATC 
ATGCGTGCAC AACCCAGTGT GGTACTGAGT GCAGCTCACA CGGTGGCTGC TAGAATGTCC 
CCCTTCGTGC GCCAGATGGA CTTTGCCATT GACTGGACAG CTGTGGAGGC TGGTCGTGCG 
CTCTACAGGC AGGGAGACCG CTCCGACTGC ACCTACATTG TACTCAATGG ACGTCTCCGT 
AGTGTCATCC AACGAGGCAG TGGCAAGAAG GAGCTAGTTG GGGAGTATGG TCGAGGGGAT 
CTCATTGGCG TGGTGGAGGC ACTGACCCGG CAGCCACGAG CCACCACTGT ACACGCAGTG 
CGAGACACTG AGCTGGCCAA GCTTCCCGAG GGCACCTTAG GCCACATCAA ACGTCGGTAC 
CCACAGGTTG TGACCCGCCT TATTCATCTG CTAAGCCAGA AAATTCTAGG CAATCTGCAG 
CAGTTGCAAG GACCCTTTCC AGGCTCCGGG CTCAGTGTTC CGCAGCACTC GGAACTGACC 
AACCCAGCCA GCAATCTGTC TACTGTAGCT ATCCTCCCTG TGTGTGCTGA GGTGCCCATG 
ATGGCCTTTA CTCTGGAACT GCAGCATGCT CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT 
AACAGTGACG TCATCCGGGC ACTCCTGGGG GCTTCAGCAC TGGATAGCAT TCAAGAATTC 
CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA GATGCCCATC GCATTGTGCT CTACCAAACT 
GACACATCCC TGACGCCTTG GACCGTCCGT TGTCTGCGCC AGGCCGACTG CATCCTCATT 
GTGGGCCTGG GGGACCAGGA ACCCACTGTT GGCCAGCTGG AGCAAATGTT AGAGAACACT 
GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG CTGCACCGGG AGGAAGGCCC TGGTCCCACG 
CGCACTGTAG AGTGGCTCAA CATGCGCAGC TGGTGCTCAG GGCACCTGCA CCTGCGCTGT 
CCTCGCCGCC TCTTCTCACG GCGCAGCCCA GCGAAACTTC ATGAGCTGTA TGAGAAGGTT 
TTCTCCAGGC GTGCAGACCG TCACAGCGAC TTCTCCCGCT TGGCACGAGT ACTCACAGGA 
AACACTATTG CTCTGGTGCT GGGTGGGGGC GGAGCCAGAG GCTGCTCGCA TATTGGGGTG 
CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA GTCGACCTTG TGGGAGGCAC ATCCATAGGT 
TCCTTCATTG GGGCCTTGTA TGCCGAGGAA CGCAGCGCCA GCCGAACTAA ACAACGAGCC 
CGGGAGTGGG CCAAGAGCAT GACTTCTGTA CTGGAGCCTG TATTGGACCT CACATATCCT 
GTCACCTCCA TGTTTACTGG CTCAGCCTTT AACAGAAGTA TCCACCGTGT CTTCCAGGAT 
AAGCAGATTG AGGACCTGTG GCTGCCTTAC TTCAATGTGA CCACAGACAT CACTGCCTCT 
GCCATGCGCG TCCACAAAGA TGGCTCCCTG TGGCGGTATG TACGTGCCAG CATGACGCTC 
TCGGGCTACC TACCCCCGCT GTGCGACCCA AAGGATGGGC ACCTGCTCAT GGATGGTGGC 
TACATCAACA ACCTGCCAGC GGATATTGCC CGAAGCATGG GAGCCAAAAC GGTCATTGCC 
ATCGACGTTG GAAGCCAGGA TGAGACAGAT CTCAGCACCT ATGGGGACAG CCTGTCTGGC 
TGGTGGTTGC TGTGGAAAAG GCTAAACCCC TGGGCAGACA AGGTAAAGGT TCCAGACATG 
GCTGAGATCC AGTCCCGCTT GGCGTACGTG TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG 
TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA TCCATTGACT GCTTCAAGAC CATGGACTTT 
GGAAAGTTTG ACCAGATCTA TGATGTGGGT TACCAGTATG GGAAGGCTGT CTTTGGAGGC 
TGGACCCGTG GTGAAGTCAT TGAGAAAATG CTCACAGACC GGAGATCTAC AGACCTTAAT 
GAGAGTCGCC GTGCAGATAT ACTTGCCTTC CCAAGTTCTG GGTTCACTGA CTTGGCTGAG 
ATTGTGTCCC GGATCGAGCC ACCAACAAGC TACGTCTCTG ATGGCTGTGC TGATGGGGAG 
GAGTCGGATT GCTTGACAGA GTATGAGGAA GATGCAGGAC CAGACTGCTC AAGAGATGAA 
GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC 
ACACTCCGGC AACGACGCTT TCTGCCTCAG GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508885.1
CDS	149..4228
Translation	MGTPSHELNTTSSGAEVIQKTLEEGLGRRICVAQPVPFVPQVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508822.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508822.3

ORF Insert Sequence:

ATGGGGACAC CGAGTCACGA ACTGAATACA ACCTCCTCCG GGGCAGAGGT GATTCAAAAG 
ACCCTGGAGG AGGGCTTGGG GAGACGGATA TGCGTTGCCC AGCCTGTGCC GTTCGTTCCG 
CAGGTGCTGG GCGTGATGAT CGGGGCCGGA GTGGCGGTGC TGGTCACCGC CGTGCTCATC 
CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG AAAACTCCAG CCCCGGAGGG TCCCCGGTAT 
CGGTTCCGGA AGCGGGACAA AGTGCTTTTC TATGGCCGGA AGATTATGCG GAAGGTGTCA 
CAGTCCACTT CTTCCCTGGT GGACACATCA GTCTCCACCA CTTCCCGGCC CCGCATGAAG 
AAGAAACTGA AGATGCTTAA CATTGCCAAG AAGATCTTAC GTATCCAAAA GGAGACTCCG 
ACCCTACAAC GGAAGGAGCC CCCACCTTCA GTGCTGGAGG CTGACCTCAC AGAGGGAGAC 
CTGGCCAATT CCCACCTACC CTCTGAGGTA CTCTACATGC TTAAGAATGT TCGGGTGCTA 
GGTCACTTTG AGAAGCCGCT CTTCCTGGAG CTCTGCCGGC ACATGGTCTT CCAGCGTCTC 
GGGCAGGGGG ACTATGTTTT TCGGCCCGGT CAACCAGATG CCAGCATCTA TGTGGTTCAA 
GATGGGCTGC TGGAGCTCTG CCTACCAGGG CCTGATGGGA AGGAGTGTGT GGTGAAGGAG 
GTGGTTCCTG GAGACAGCGT CAACAGCCTT CTGAGCATCC TTGATGTCAT CACGGGTCAC 
CAGCATCCCC AGCGGACTGT GTCAGCCAGG GCTGCACGGG ACTCCACAGT GCTGAGACTC 
CCAGTAGAAG CCTTCTCTGC TGTCTTCACC AAGTACCCTG AGAGTTTGGT GCGAGTGGTA 
CAGATCATCA TGGTAAGGCT GCAGAGAGTC ACCTTCCTGG CGCTTCACAA TTACCTGGGT 
CTGACCAATG AACTCTTCAG TCATGAGATC CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC 
CTCCCGACCC GAACCAGCCC TGTCCGTGGC TCTAAACGGG TAGTCAGCAC CTCAGGTACT 
GAGGACACCT CAAAGGAGAC CTCTGGCCGG CCCCTGGACT CCATTGGAGC TCCTCTGCCT 
GGACCTGCAG GGGATCCCGT GAAGCCCACA TCCTTAGAAG CACCCCCAGC CCCGTTACTG 
AGCCGCTGCA TCTCCATGCC AGTAGACATC TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT 
TTTGACATGG CCTATGAACG TGGACGGATA TCTGTTTCTC TTCAAGAAGA GGCATCTGGG 
GGACCTCAGA CAGCATCCCC TAGGACCCCC ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT 
GCCTGTGAAT ATAGCTACTG TGAGGATGAG TCAGCCACAG GCGGATGTCC CTTTGGGCCC 
TACCAGGGCC GCCAGACAAG CAGCATCTTT GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG 
ATGCGGATTG AGGACCCCTC TCTACTGAAC AGCAGAGTCT TGCTACATCA TGCCAAAGCC 
GGCACCATCA TAGCCCGCCA AGGGGACCAG GATGTGAGCC TGCACTTTGT GCTCTGGGGC 
TGCCTGCACG TGTACCAGCG CATGATTGAC AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA 
CAGCCAGGGG AGCTGGTGGG GCAACTGGCG GTGCTCACTG GGGAACCTCT CATCTTCACA 
CTACGTGCCC AGAGAGACTG CACCTTCCTG CGGATCTCCA AATCCCACTT CTATGAGATC 
ATGCGTGCAC AACCCAGTGT GGTACTGAGT GCAGCTCACA CGGTGGCTGC TAGAATGTCC 
CCCTTCGTGC GCCAGATGGA CTTTGCCATT GACTGGACAG CTGTGGAGGC TGGTCGTGCG 
CTCTACAGGC AGGGAGACCG CTCCGACTGC ACCTACATTG TACTCAATGG ACGTCTCCGT 
AGTGTCATCC AACGAGGCAG TGGCAAGAAG GAGCTAGTTG GGGAGTATGG TCGAGGGGAT 
CTCATTGGCG TGGTGGAGGC ACTGACCCGG CAGCCACGAG CCACCACTGT ACACGCAGTG 
CGAGACACTG AGCTGGCCAA GCTTCCCGAG GGCACCTTAG GCCACATCAA ACGTCGGTAC 
CCACAGGTTG TGACCCGCCT TATTCATCTG CTAAGCCAGA AAATTCTAGG CAATCTGCAG 
CAGTTGCAAG GACCCTTTCC AGGCTCCGGG CTCAGTGTTC CGCAGCACTC GGAACTGACC 
AACCCAGCCA GCAATCTGTC TACTGTAGCT ATCCTCCCTG TGTGTGCTGA GGTGCCCATG 
ATGGCCTTTA CTCTGGAACT GCAGCATGCT CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT 
AACAGTGACG TCATCCGGGC ACTCCTGGGG GCTTCAGCAC TGGATAGCAT TCAAGAATTC 
CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA GATGCCCATC GCATTGTGCT CTACCAAACT 
GACACATCCC TGACGCCTTG GACCGTCCGT TGTCTGCGCC AGGCCGACTG CATCCTCATT 
GTGGGCCTGG GGGACCAGGA ACCCACTGTT GGCCAGCTGG AGCAAATGTT AGAGAACACT 
GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG CTGCACCGGG AGGAAGGCCC TGGTCCCACG 
CGCACTGTAG AGTGGCTCAA CATGCGCAGC TGGTGCTCAG GGCACCTGCA CCTGCGCTGT 
CCTCGCCGCC TCTTCTCACG GCGCAGCCCA GCGAAACTTC ATGAGCTGTA TGAGAAGGTT 
TTCTCCAGGC GTGCAGACCG TCACAGCGAC TTCTCCCGCT TGGCACGAGT ACTCACAGGA 
AACACTATTG CTCTGGTGCT GGGTGGGGGC GGAGCCAGAG GCTGCTCGCA TATTGGGGTG 
CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA GTCGACCTTG TGGGAGGCAC ATCCATAGGT 
TCCTTCATTG GGGCCTTGTA TGCCGAGGAA CGCAGCGCCA GCCGAACTAA ACAACGAGCC 
CGGGAGTGGG CCAAGAGCAT GACTTCTGTA CTGGAGCCTG TATTGGACCT CACATATCCT 
GTCACCTCCA TGTTTACTGG CTCAGCCTTT AACAGAAGTA TCCACCGTGT CTTCCAGGAT 
AAGCAGATTG AGGACCTGTG GCTGCCTTAC TTCAATGTGA CCACAGACAT CACTGCCTCT 
GCCATGCGCG TCCACAAAGA TGGCTCCCTG TGGCGGTATG TACGTGCCAG CATGACGCTC 
TCGGGCTACC TACCCCCGCT GTGCGACCCA AAGGATGGGC ACCTGCTCAT GGATGGTGGC 
TACATCAACA ACCTGCCAGC GGATATTGCC CGAAGCATGG GAGCCAAAAC GGTCATTGCC 
ATCGACGTTG GAAGCCAGGA TGAGACAGAT CTCAGCACCT ATGGGGACAG CCTGTCTGGC 
TGGTGGTTGC TGTGGAAAAG GCTAAACCCC TGGGCAGACA AGGTAAAGGT TCCAGACATG 
GCTGAGATCC AGTCCCGCTT GGCGTACGTG TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG 
TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA TCCATTGACT GCTTCAAGAC CATGGACTTT 
GGAAAGTTTG ACCAGATCTA TGATGTGGGT TACCAGTATG GGAAGGCTGT CTTTGGAGGC 
TGGACCCGTG GTGAAGTCAT TGAGAAAATG CTCACAGACC GGAGATCTAC AGACCTTAAT 
GAGAGTCGCC GTGCAGATAT ACTTGCCTTC CCAAGTTCTG GGTTCACTGA CTTGGCTGAG 
ATTGTGTCCC GGATCGAGCC ACCAACAAGC TACGTCTCTG ATGGCTGTGC TGATGGGGAG 
GAGTCGGATT GCTTGACAGA GTATGAGGAA GATGCAGGAC CAGACTGCTC AAGAGATGAA 
GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC 
ACACTCCGGC AACGACGCTT TCTGCCTCAG GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508823.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508886.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X2, mRNA.

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CloneID	OMu40423
Clone ID Related Accession (Same CDS sequence)	XM_006508823.3 , NM_001359124.1
Accession Version	XM_006508823.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	neuropathy target esterase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508823.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGACAC CGAGTCACGA ACTGAATACA ACCTCCTCCG GGGCAGAGGT GATTCAAAAG 
ACCCTGGAGG AGGGCTTGGG GAGACGGATA TGCGTTGCCC AGCCTGTGCC GTTCGTTCCG 
CAGGTGCTGG GCGTGATGAT CGGGGCCGGA GTGGCGGTGC TGGTCACCGC CGTGCTCATC 
CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG AAAACTCCAG CCCCGGAGGG TCCCCGGTAT 
CGGTTCCGGA AGCGGGACAA AGTGCTTTTC TATGGCCGGA AGATTATGCG GAAGGTGTCA 
CAGTCCACTT CTTCCCTGGT GGACACATCA GTCTCCACCA CTTCCCGGCC CCGCATGAAG 
AAGAAACTGA AGATGCTTAA CATTGCCAAG AAGATCTTAC GTATCCAAAA GGAGACTCCG 
ACCCTACAAC GGAAGGAGCC CCCACCTTCA GTGCTGGAGG CTGACCTCAC AGAGGGAGAC 
CTGGCCAATT CCCACCTACC CTCTGAGGTA CTCTACATGC TTAAGAATGT TCGGGTGCTA 
GGTCACTTTG AGAAGCCGCT CTTCCTGGAG CTCTGCCGGC ACATGGTCTT CCAGCGTCTC 
GGGCAGGGGG ACTATGTTTT TCGGCCCGGT CAACCAGATG CCAGCATCTA TGTGGTTCAA 
GATGGGCTGC TGGAGCTCTG CCTACCAGGG CCTGATGGGA AGGAGTGTGT GGTGAAGGAG 
GTGGTTCCTG GAGACAGCGT CAACAGCCTT CTGAGCATCC TTGATGTCAT CACGGGTCAC 
CAGCATCCCC AGCGGACTGT GTCAGCCAGG GCTGCACGGG ACTCCACAGT GCTGAGACTC 
CCAGTAGAAG CCTTCTCTGC TGTCTTCACC AAGTACCCTG AGAGTTTGGT GCGAGTGGTA 
CAGATCATCA TGGTAAGGCT GCAGAGAGTC ACCTTCCTGG CGCTTCACAA TTACCTGGGT 
CTGACCAATG AACTCTTCAG TCATGAGATC CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC 
CTCCCGACCC GAACCAGCCC TGTCCGTGGC TCTAAACGGG TAGTCAGCAC CTCAGGTACT 
GAGGACACCT CAAAGGAGAC CTCTGGCCGG CCCCTGGACT CCATTGGAGC TCCTCTGCCT 
GGACCTGCAG GGGATCCCGT GAAGCCCACA TCCTTAGAAG CACCCCCAGC CCCGTTACTG 
AGCCGCTGCA TCTCCATGCC AGTAGACATC TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT 
TTTGACATGG CCTATGAACG TGGACGGATA TCTGTTTCTC TTCAAGAAGA GGCATCTGGG 
GGACCTCAGA CAGCATCCCC TAGGACCCCC ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT 
GCCTGTGAAT ATAGCTACTG TGAGGATGAG TCAGCCACAG GCGGATGTCC CTTTGGGCCC 
TACCAGGGCC GCCAGACAAG CAGCATCTTT GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG 
ATGCGGATTG AGGACCCCTC TCTACTGAAC AGCAGAGTCT TGCTACATCA TGCCAAAGCC 
GGCACCATCA TAGCCCGCCA AGGGGACCAG GATGTGAGCC TGCACTTTGT GCTCTGGGGC 
TGCCTGCACG TGTACCAGCG CATGATTGAC AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA 
CAGCCAGGGG AGCTGGTGGG GCAACTGGCG GTGCTCACTG GGGAACCTCT CATCTTCACA 
CTACGTGCCC AGAGAGACTG CACCTTCCTG CGGATCTCCA AATCCCACTT CTATGAGATC 
ATGCGTGCAC AACCCAGTGT GGTACTGAGT GCAGCTCACA CGGTGGCTGC TAGAATGTCC 
CCCTTCGTGC GCCAGATGGA CTTTGCCATT GACTGGACAG CTGTGGAGGC TGGTCGTGCG 
CTCTACAGGC AGGGAGACCG CTCCGACTGC ACCTACATTG TACTCAATGG ACGTCTCCGT 
AGTGTCATCC AACGAGGCAG TGGCAAGAAG GAGCTAGTTG GGGAGTATGG TCGAGGGGAT 
CTCATTGGCG TGGTGGAGGC ACTGACCCGG CAGCCACGAG CCACCACTGT ACACGCAGTG 
CGAGACACTG AGCTGGCCAA GCTTCCCGAG GGCACCTTAG GCCACATCAA ACGTCGGTAC 
CCACAGGTTG TGACCCGCCT TATTCATCTG CTAAGCCAGA AAATTCTAGG CAATCTGCAG 
CAGTTGCAAG GACCCTTTCC AGGCTCCGGG CTCAGTGTTC CGCAGCACTC GGAACTGACC 
AACCCAGCCA GCAATCTGTC TACTGTAGCT ATCCTCCCTG TGTGTGCTGA GGTGCCCATG 
ATGGCCTTTA CTCTGGAACT GCAGCATGCT CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT 
AACAGTGACG TCATCCGGGC ACTCCTGGGG GCTTCAGCAC TGGATAGCAT TCAAGAATTC 
CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA GATGCCCATC GCATTGTGCT CTACCAAACT 
GACACATCCC TGACGCCTTG GACCGTCCGT TGTCTGCGCC AGGCCGACTG CATCCTCATT 
GTGGGCCTGG GGGACCAGGA ACCCACTGTT GGCCAGCTGG AGCAAATGTT AGAGAACACT 
GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG CTGCACCGGG AGGAAGGCCC TGGTCCCACG 
CGCACTGTAG AGTGGCTCAA CATGCGCAGC TGGTGCTCAG GGCACCTGCA CCTGCGCTGT 
CCTCGCCGCC TCTTCTCACG GCGCAGCCCA GCGAAACTTC ATGAGCTGTA TGAGAAGGTT 
TTCTCCAGGC GTGCAGACCG TCACAGCGAC TTCTCCCGCT TGGCACGAGT ACTCACAGGA 
AACACTATTG CTCTGGTGCT GGGTGGGGGC GGAGCCAGAG GCTGCTCGCA TATTGGGGTG 
CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA GTCGACCTTG TGGGAGGCAC ATCCATAGGT 
TCCTTCATTG GGGCCTTGTA TGCCGAGGAA CGCAGCGCCA GCCGAACTAA ACAACGAGCC 
CGGGAGTGGG CCAAGAGCAT GACTTCTGTA CTGGAGCCTG TATTGGACCT CACATATCCT 
GTCACCTCCA TGTTTACTGG CTCAGCCTTT AACAGAAGTA TCCACCGTGT CTTCCAGGAT 
AAGCAGATTG AGGACCTGTG GCTGCCTTAC TTCAATGTGA CCACAGACAT CACTGCCTCT 
GCCATGCGCG TCCACAAAGA TGGCTGCGTG TGGCGTTACG TCCGGGCCAG TGCCTCCTAC 
TGTCCCTACC TGCCCCCACT CTGCGACCCC AAGGACGGGC ACCTGCTGGT GGACGGGTGT 
TACGTTAACA ACGTTCCAGC GGATATTGCC CGAAGCATGG GAGCCAAAAC GGTCATTGCC 
ATCGACGTTG GAAGCCAGGA TGAGACAGAT CTCAGCACCT ATGGGGACAG CCTGTCTGGC 
TGGTGGTTGC TGTGGAAAAG GCTAAACCCC TGGGCAGACA AGGTAAAGGT TCCAGACATG 
GCTGAGATCC AGTCCCGCTT GGCGTACGTG TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG 
TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA TCCATTGACT GCTTCAAGAC CATGGACTTT 
GGAAAGTTTG ACCAGATCTA TGATGTGGGT TACCAGTATG GGAAGGCTGT CTTTGGAGGC 
TGGACCCGTG GTGAAGTCAT TGAGAAAATG CTCACAGACC GGAGATCTAC AGACCTTAAT 
GAGAGTCGCC GTGCAGATAT ACTTGCCTTC CCAAGTTCTG GGTTCACTGA CTTGGCTGAG 
ATTGTGTCCC GGATCGAGCC ACCAACAAGC TACGTCTCTG ATGGCTGTGC TGATGGGGAG 
GAGTCGGATT GCTTGACAGA GTATGAGGAA GATGCAGGAC CAGACTGCTC AAGAGATGAA 
GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC 
ACACTCCGGC AACGACGCTT TCTGCCTCAG GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508886.1
CDS	151..4230
Translation	MGTPSHELNTTSSGAEVIQKTLEEGLGRRICVAQPVPFVPQVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGCVWRYVRASASYCPYLPPLCDPKDGHLLVDGCYVNNVPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508823.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508823.3

ORF Insert Sequence:

ATGGGGACAC CGAGTCACGA ACTGAATACA ACCTCCTCCG GGGCAGAGGT GATTCAAAAG 
ACCCTGGAGG AGGGCTTGGG GAGACGGATA TGCGTTGCCC AGCCTGTGCC GTTCGTTCCG 
CAGGTGCTGG GCGTGATGAT CGGGGCCGGA GTGGCGGTGC TGGTCACCGC CGTGCTCATC 
CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG AAAACTCCAG CCCCGGAGGG TCCCCGGTAT 
CGGTTCCGGA AGCGGGACAA AGTGCTTTTC TATGGCCGGA AGATTATGCG GAAGGTGTCA 
CAGTCCACTT CTTCCCTGGT GGACACATCA GTCTCCACCA CTTCCCGGCC CCGCATGAAG 
AAGAAACTGA AGATGCTTAA CATTGCCAAG AAGATCTTAC GTATCCAAAA GGAGACTCCG 
ACCCTACAAC GGAAGGAGCC CCCACCTTCA GTGCTGGAGG CTGACCTCAC AGAGGGAGAC 
CTGGCCAATT CCCACCTACC CTCTGAGGTA CTCTACATGC TTAAGAATGT TCGGGTGCTA 
GGTCACTTTG AGAAGCCGCT CTTCCTGGAG CTCTGCCGGC ACATGGTCTT CCAGCGTCTC 
GGGCAGGGGG ACTATGTTTT TCGGCCCGGT CAACCAGATG CCAGCATCTA TGTGGTTCAA 
GATGGGCTGC TGGAGCTCTG CCTACCAGGG CCTGATGGGA AGGAGTGTGT GGTGAAGGAG 
GTGGTTCCTG GAGACAGCGT CAACAGCCTT CTGAGCATCC TTGATGTCAT CACGGGTCAC 
CAGCATCCCC AGCGGACTGT GTCAGCCAGG GCTGCACGGG ACTCCACAGT GCTGAGACTC 
CCAGTAGAAG CCTTCTCTGC TGTCTTCACC AAGTACCCTG AGAGTTTGGT GCGAGTGGTA 
CAGATCATCA TGGTAAGGCT GCAGAGAGTC ACCTTCCTGG CGCTTCACAA TTACCTGGGT 
CTGACCAATG AACTCTTCAG TCATGAGATC CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC 
CTCCCGACCC GAACCAGCCC TGTCCGTGGC TCTAAACGGG TAGTCAGCAC CTCAGGTACT 
GAGGACACCT CAAAGGAGAC CTCTGGCCGG CCCCTGGACT CCATTGGAGC TCCTCTGCCT 
GGACCTGCAG GGGATCCCGT GAAGCCCACA TCCTTAGAAG CACCCCCAGC CCCGTTACTG 
AGCCGCTGCA TCTCCATGCC AGTAGACATC TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT 
TTTGACATGG CCTATGAACG TGGACGGATA TCTGTTTCTC TTCAAGAAGA GGCATCTGGG 
GGACCTCAGA CAGCATCCCC TAGGACCCCC ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT 
GCCTGTGAAT ATAGCTACTG TGAGGATGAG TCAGCCACAG GCGGATGTCC CTTTGGGCCC 
TACCAGGGCC GCCAGACAAG CAGCATCTTT GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG 
ATGCGGATTG AGGACCCCTC TCTACTGAAC AGCAGAGTCT TGCTACATCA TGCCAAAGCC 
GGCACCATCA TAGCCCGCCA AGGGGACCAG GATGTGAGCC TGCACTTTGT GCTCTGGGGC 
TGCCTGCACG TGTACCAGCG CATGATTGAC AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA 
CAGCCAGGGG AGCTGGTGGG GCAACTGGCG GTGCTCACTG GGGAACCTCT CATCTTCACA 
CTACGTGCCC AGAGAGACTG CACCTTCCTG CGGATCTCCA AATCCCACTT CTATGAGATC 
ATGCGTGCAC AACCCAGTGT GGTACTGAGT GCAGCTCACA CGGTGGCTGC TAGAATGTCC 
CCCTTCGTGC GCCAGATGGA CTTTGCCATT GACTGGACAG CTGTGGAGGC TGGTCGTGCG 
CTCTACAGGC AGGGAGACCG CTCCGACTGC ACCTACATTG TACTCAATGG ACGTCTCCGT 
AGTGTCATCC AACGAGGCAG TGGCAAGAAG GAGCTAGTTG GGGAGTATGG TCGAGGGGAT 
CTCATTGGCG TGGTGGAGGC ACTGACCCGG CAGCCACGAG CCACCACTGT ACACGCAGTG 
CGAGACACTG AGCTGGCCAA GCTTCCCGAG GGCACCTTAG GCCACATCAA ACGTCGGTAC 
CCACAGGTTG TGACCCGCCT TATTCATCTG CTAAGCCAGA AAATTCTAGG CAATCTGCAG 
CAGTTGCAAG GACCCTTTCC AGGCTCCGGG CTCAGTGTTC CGCAGCACTC GGAACTGACC 
AACCCAGCCA GCAATCTGTC TACTGTAGCT ATCCTCCCTG TGTGTGCTGA GGTGCCCATG 
ATGGCCTTTA CTCTGGAACT GCAGCATGCT CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT 
AACAGTGACG TCATCCGGGC ACTCCTGGGG GCTTCAGCAC TGGATAGCAT TCAAGAATTC 
CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA GATGCCCATC GCATTGTGCT CTACCAAACT 
GACACATCCC TGACGCCTTG GACCGTCCGT TGTCTGCGCC AGGCCGACTG CATCCTCATT 
GTGGGCCTGG GGGACCAGGA ACCCACTGTT GGCCAGCTGG AGCAAATGTT AGAGAACACT 
GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG CTGCACCGGG AGGAAGGCCC TGGTCCCACG 
CGCACTGTAG AGTGGCTCAA CATGCGCAGC TGGTGCTCAG GGCACCTGCA CCTGCGCTGT 
CCTCGCCGCC TCTTCTCACG GCGCAGCCCA GCGAAACTTC ATGAGCTGTA TGAGAAGGTT 
TTCTCCAGGC GTGCAGACCG TCACAGCGAC TTCTCCCGCT TGGCACGAGT ACTCACAGGA 
AACACTATTG CTCTGGTGCT GGGTGGGGGC GGAGCCAGAG GCTGCTCGCA TATTGGGGTG 
CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA GTCGACCTTG TGGGAGGCAC ATCCATAGGT 
TCCTTCATTG GGGCCTTGTA TGCCGAGGAA CGCAGCGCCA GCCGAACTAA ACAACGAGCC 
CGGGAGTGGG CCAAGAGCAT GACTTCTGTA CTGGAGCCTG TATTGGACCT CACATATCCT 
GTCACCTCCA TGTTTACTGG CTCAGCCTTT AACAGAAGTA TCCACCGTGT CTTCCAGGAT 
AAGCAGATTG AGGACCTGTG GCTGCCTTAC TTCAATGTGA CCACAGACAT CACTGCCTCT 
GCCATGCGCG TCCACAAAGA TGGCTGCGTG TGGCGTTACG TCCGGGCCAG TGCCTCCTAC 
TGTCCCTACC TGCCCCCACT CTGCGACCCC AAGGACGGGC ACCTGCTGGT GGACGGGTGT 
TACGTTAACA ACGTTCCAGC GGATATTGCC CGAAGCATGG GAGCCAAAAC GGTCATTGCC 
ATCGACGTTG GAAGCCAGGA TGAGACAGAT CTCAGCACCT ATGGGGACAG CCTGTCTGGC 
TGGTGGTTGC TGTGGAAAAG GCTAAACCCC TGGGCAGACA AGGTAAAGGT TCCAGACATG 
GCTGAGATCC AGTCCCGCTT GGCGTACGTG TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG 
TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA TCCATTGACT GCTTCAAGAC CATGGACTTT 
GGAAAGTTTG ACCAGATCTA TGATGTGGGT TACCAGTATG GGAAGGCTGT CTTTGGAGGC 
TGGACCCGTG GTGAAGTCAT TGAGAAAATG CTCACAGACC GGAGATCTAC AGACCTTAAT 
GAGAGTCGCC GTGCAGATAT ACTTGCCTTC CCAAGTTCTG GGTTCACTGA CTTGGCTGAG 
ATTGTGTCCC GGATCGAGCC ACCAACAAGC TACGTCTCTG ATGGCTGTGC TGATGGGGAG 
GAGTCGGATT GCTTGACAGA GTATGAGGAA GATGCAGGAC CAGACTGCTC AAGAGATGAA 
GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC 
ACACTCCGGC AACGACGCTT TCTGCCTCAG GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508824.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508887.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X3, mRNA.

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CloneID	OMu40424
Clone ID Related Accession (Same CDS sequence)	XM_006508824.4 , XM_006508824.3
Accession Version	XM_006508824.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508824.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGACAC CGAGTCACGA ACTGAATACA ACCTCCTCCG GGGCAGAGGT GATTCAAAAG 
ACCCTGGAGG AGGGCTTGGG GAGACGGATA TGCGTTGCCC AGCCTGTGCC GTTCGTTCCG 
CAGGTGCTGG GCGTGATGAT CGGGGCCGGA GTGGCGGTGC TGGTCACCGC CGTGCTCATC 
CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG AAAACTCCAG CCCCGGAGGG TCCCCGGTAT 
CGGTTCCGGA AGCGGGACAA AGTGCTTTTC TATGGCCGGA AGATTATGCG GAAGGTGTCA 
CAGTCCACTT CTTCCCTGGT GGACACATCA GTCTCCACCA CTTCCCGGCC CCGCATGAAG 
AAGAAACTGA AGATGCTTAA CATTGCCAAG AAGATCTTAC GTATCCAAAA GGAGACTCCG 
ACCCTACAAC GGAAGGAGCC CCCACCTTCA GTGCTGGAGG CTGACCTCAC AGAGGGAGAC 
CTGGCCAATT CCCACCTACC CTCTGAGGTA CTCTACATGC TTAAGAATGT TCGGGTGCTA 
GGTCACTTTG AGAAGCCGCT CTTCCTGGAG CTCTGCCGGC ACATGGTCTT CCAGCGTCTC 
GGGCAGGGGG ACTATGTTTT TCGGCCCGGT CAACCAGATG CCAGCATCTA TGTGGTTCAA 
GATGGGCTGC TGGAGCTCTG CCTACCAGGG CCTGATGGGA AGGAGTGTGT GGTGAAGGAG 
GTGGTTCCTG GAGACAGCGT CAACAGCCTT CTGAGCATCC TTGATGTCAT CACGGGTCAC 
CAGCATCCCC AGCGGACTGT GTCAGCCAGG GCTGCACGGG ACTCCACAGT GCTGAGACTC 
CCAGTAGAAG CCTTCTCTGC TGTCTTCACC AAGTACCCTG AGAGTTTGGT GCGAGTGGTA 
CAGATCATCA TGGTAAGGCT GCAGAGAGTC ACCTTCCTGG CGCTTCACAA TTACCTGGGT 
CTGACCAATG AACTCTTCAG TCATGAGATC CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC 
CTCCCGACCC GAACCAGCCC TGTCCGTGGC TCTAAACGGG TAGTCAGCAC CTCAGGTACT 
GAGGACACCT CAAAGGAGAC CTCTGGCCGG CCCCTGGACT CCATTGGAGC TCCTCTGCCT 
GGACCTGCAG GGGATCCCGT GAAGCCCACA TCCTTAGAAG CACCCCCAGC CCCGTTACTG 
AGCCGCTGCA TCTCCATGCC AGTAGACATC TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT 
TTTGACATGG CCTATGAACG TGGACGGATA TCTGTTTCTC TTCAAGAAGA GGCATCTGGG 
GGACCTCAGA CAGCATCCCC TAGGGAGCTC CGGGAGCAGC CAGCGGGTGC CTGTGAATAT 
AGCTACTGTG AGGATGAGTC AGCCACAGGC GGATGTCCCT TTGGGCCCTA CCAGGGCCGC 
CAGACAAGCA GCATCTTTGA GGCTGCAAAG AGAGAGCTAG CCAAGCTGAT GCGGATTGAG 
GACCCCTCTC TACTGAACAG CAGAGTCTTG CTACATCATG CCAAAGCCGG CACCATCATA 
GCCCGCCAAG GGGACCAGGA TGTGAGCCTG CACTTTGTGC TCTGGGGCTG CCTGCACGTG 
TACCAGCGCA TGATTGACAA GGCTGAGGAA GTGTGCCTAT TTGTGGCACA GCCAGGGGAG 
CTGGTGGGGC AACTGGCGGT GCTCACTGGG GAACCTCTCA TCTTCACACT ACGTGCCCAG 
AGAGACTGCA CCTTCCTGCG GATCTCCAAA TCCCACTTCT ATGAGATCAT GCGTGCACAA 
CCCAGTGTGG TACTGAGTGC AGCTCACACG GTGGCTGCTA GAATGTCCCC CTTCGTGCGC 
CAGATGGACT TTGCCATTGA CTGGACAGCT GTGGAGGCTG GTCGTGCGCT CTACAGGCAG 
GGAGACCGCT CCGACTGCAC CTACATTGTA CTCAATGGAC GTCTCCGTAG TGTCATCCAA 
CGAGGCAGTG GCAAGAAGGA GCTAGTTGGG GAGTATGGTC GAGGGGATCT CATTGGCGTG 
GTGGAGGCAC TGACCCGGCA GCCACGAGCC ACCACTGTAC ACGCAGTGCG AGACACTGAG 
CTGGCCAAGC TTCCCGAGGG CACCTTAGGC CACATCAAAC GTCGGTACCC ACAGGTTGTG 
ACCCGCCTTA TTCATCTGCT AAGCCAGAAA ATTCTAGGCA ATCTGCAGCA GTTGCAAGGA 
CCCTTTCCAG GCTCCGGGCT CAGTGTTCCG CAGCACTCGG AACTGACCAA CCCAGCCAGC 
AATCTGTCTA CTGTAGCTAT CCTCCCTGTG TGTGCTGAGG TGCCCATGAT GGCCTTTACT 
CTGGAACTGC AGCATGCTCT GCAAGCTATT GGTCCCACGC TCCTCCTTAA CAGTGACGTC 
ATCCGGGCAC TCCTGGGGGC TTCAGCACTG GATAGCATTC AAGAATTCCG GCTGTCAGGG 
TGGTTGGCCC AGCAGGAAGA TGCCCATCGC ATTGTGCTCT ACCAAACTGA CACATCCCTG 
ACGCCTTGGA CCGTCCGTTG TCTGCGCCAG GCCGACTGCA TCCTCATTGT GGGCCTGGGG 
GACCAGGAAC CCACTGTTGG CCAGCTGGAG CAAATGTTAG AGAACACTGC TGTCCGTGCC 
TTGAAGCAAC TGGTTTTGCT GCACCGGGAG GAAGGCCCTG GTCCCACGCG CACTGTAGAG 
TGGCTCAACA TGCGCAGCTG GTGCTCAGGG CACCTGCACC TGCGCTGTCC TCGCCGCCTC 
TTCTCACGGC GCAGCCCAGC GAAACTTCAT GAGCTGTATG AGAAGGTTTT CTCCAGGCGT 
GCAGACCGTC ACAGCGACTT CTCCCGCTTG GCACGAGTAC TCACAGGAAA CACTATTGCT 
CTGGTGCTGG GTGGGGGCGG AGCCAGAGGC TGCTCGCATA TTGGGGTGCT GAAGGCATTA 
GAGGAGGCAG GAGTCCCAGT CGACCTTGTG GGAGGCACAT CCATAGGTTC CTTCATTGGG 
GCCTTGTATG CCGAGGAACG CAGCGCCAGC CGAACTAAAC AACGAGCCCG GGAGTGGGCC 
AAGAGCATGA CTTCTGTACT GGAGCCTGTA TTGGACCTCA CATATCCTGT CACCTCCATG 
TTTACTGGCT CAGCCTTTAA CAGAAGTATC CACCGTGTCT TCCAGGATAA GCAGATTGAG 
GACCTGTGGC TGCCTTACTT CAATGTGACC ACAGACATCA CTGCCTCTGC CATGCGCGTC 
CACAAAGATG GCTCCCTGTG GCGGTATGTA CGTGCCAGCA TGACGCTCTC GGGCTACCTA 
CCCCCGCTGT GCGACCCAAA GGATGGGCAC CTGCTCATGG ATGGTGGCTA CATCAACAAC 
CTGCCAGCGG ATATTGCCCG AAGCATGGGA GCCAAAACGG TCATTGCCAT CGACGTTGGA 
AGCCAGGATG AGACAGATCT CAGCACCTAT GGGGACAGCC TGTCTGGCTG GTGGTTGCTG 
TGGAAAAGGC TAAACCCCTG GGCAGACAAG GTAAAGGTTC CAGACATGGC TGAGATCCAG 
TCCCGCTTGG CGTACGTGTC CTGTGTGCGG CAGCTAGAAG TCGTCAAGTC CAGCTCCTAT 
TGCGAGTACC TTCGTCCATC CATTGACTGC TTCAAGACCA TGGACTTTGG AAAGTTTGAC 
CAGATCTATG ATGTGGGTTA CCAGTATGGG AAGGCTGTCT TTGGAGGCTG GACCCGTGGT 
GAAGTCATTG AGAAAATGCT CACAGACCGG AGATCTACAG ACCTTAATGA GAGTCGCCGT 
GCAGATATAC TTGCCTTCCC AAGTTCTGGG TTCACTGACT TGGCTGAGAT TGTGTCCCGG 
ATCGAGCCAC CAACAAGCTA CGTCTCTGAT GGCTGTGCTG ATGGGGAGGA GTCGGATTGC 
TTGACAGAGT ATGAGGAAGA TGCAGGACCA GACTGCTCAA GAGATGAAGG GGGCTCCCCT 
GAGGGTGCCA GCCCTAGCAC TGCCTCAGAG GTGGAAGAAG AGAAGTCCAC ACTCCGGCAA 
CGACGCTTTC TGCCTCAGGA GACTCCCAGC TCAGTCGCAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508887.1
CDS	149..4216
Translation	MGTPSHELNTTSSGAEVIQKTLEEGLGRRICVAQPVPFVPQVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508824.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508824.3

ORF Insert Sequence:

ATGGGGACAC CGAGTCACGA ACTGAATACA ACCTCCTCCG GGGCAGAGGT GATTCAAAAG 
ACCCTGGAGG AGGGCTTGGG GAGACGGATA TGCGTTGCCC AGCCTGTGCC GTTCGTTCCG 
CAGGTGCTGG GCGTGATGAT CGGGGCCGGA GTGGCGGTGC TGGTCACCGC CGTGCTCATC 
CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG AAAACTCCAG CCCCGGAGGG TCCCCGGTAT 
CGGTTCCGGA AGCGGGACAA AGTGCTTTTC TATGGCCGGA AGATTATGCG GAAGGTGTCA 
CAGTCCACTT CTTCCCTGGT GGACACATCA GTCTCCACCA CTTCCCGGCC CCGCATGAAG 
AAGAAACTGA AGATGCTTAA CATTGCCAAG AAGATCTTAC GTATCCAAAA GGAGACTCCG 
ACCCTACAAC GGAAGGAGCC CCCACCTTCA GTGCTGGAGG CTGACCTCAC AGAGGGAGAC 
CTGGCCAATT CCCACCTACC CTCTGAGGTA CTCTACATGC TTAAGAATGT TCGGGTGCTA 
GGTCACTTTG AGAAGCCGCT CTTCCTGGAG CTCTGCCGGC ACATGGTCTT CCAGCGTCTC 
GGGCAGGGGG ACTATGTTTT TCGGCCCGGT CAACCAGATG CCAGCATCTA TGTGGTTCAA 
GATGGGCTGC TGGAGCTCTG CCTACCAGGG CCTGATGGGA AGGAGTGTGT GGTGAAGGAG 
GTGGTTCCTG GAGACAGCGT CAACAGCCTT CTGAGCATCC TTGATGTCAT CACGGGTCAC 
CAGCATCCCC AGCGGACTGT GTCAGCCAGG GCTGCACGGG ACTCCACAGT GCTGAGACTC 
CCAGTAGAAG CCTTCTCTGC TGTCTTCACC AAGTACCCTG AGAGTTTGGT GCGAGTGGTA 
CAGATCATCA TGGTAAGGCT GCAGAGAGTC ACCTTCCTGG CGCTTCACAA TTACCTGGGT 
CTGACCAATG AACTCTTCAG TCATGAGATC CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC 
CTCCCGACCC GAACCAGCCC TGTCCGTGGC TCTAAACGGG TAGTCAGCAC CTCAGGTACT 
GAGGACACCT CAAAGGAGAC CTCTGGCCGG CCCCTGGACT CCATTGGAGC TCCTCTGCCT 
GGACCTGCAG GGGATCCCGT GAAGCCCACA TCCTTAGAAG CACCCCCAGC CCCGTTACTG 
AGCCGCTGCA TCTCCATGCC AGTAGACATC TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT 
TTTGACATGG CCTATGAACG TGGACGGATA TCTGTTTCTC TTCAAGAAGA GGCATCTGGG 
GGACCTCAGA CAGCATCCCC TAGGGAGCTC CGGGAGCAGC CAGCGGGTGC CTGTGAATAT 
AGCTACTGTG AGGATGAGTC AGCCACAGGC GGATGTCCCT TTGGGCCCTA CCAGGGCCGC 
CAGACAAGCA GCATCTTTGA GGCTGCAAAG AGAGAGCTAG CCAAGCTGAT GCGGATTGAG 
GACCCCTCTC TACTGAACAG CAGAGTCTTG CTACATCATG CCAAAGCCGG CACCATCATA 
GCCCGCCAAG GGGACCAGGA TGTGAGCCTG CACTTTGTGC TCTGGGGCTG CCTGCACGTG 
TACCAGCGCA TGATTGACAA GGCTGAGGAA GTGTGCCTAT TTGTGGCACA GCCAGGGGAG 
CTGGTGGGGC AACTGGCGGT GCTCACTGGG GAACCTCTCA TCTTCACACT ACGTGCCCAG 
AGAGACTGCA CCTTCCTGCG GATCTCCAAA TCCCACTTCT ATGAGATCAT GCGTGCACAA 
CCCAGTGTGG TACTGAGTGC AGCTCACACG GTGGCTGCTA GAATGTCCCC CTTCGTGCGC 
CAGATGGACT TTGCCATTGA CTGGACAGCT GTGGAGGCTG GTCGTGCGCT CTACAGGCAG 
GGAGACCGCT CCGACTGCAC CTACATTGTA CTCAATGGAC GTCTCCGTAG TGTCATCCAA 
CGAGGCAGTG GCAAGAAGGA GCTAGTTGGG GAGTATGGTC GAGGGGATCT CATTGGCGTG 
GTGGAGGCAC TGACCCGGCA GCCACGAGCC ACCACTGTAC ACGCAGTGCG AGACACTGAG 
CTGGCCAAGC TTCCCGAGGG CACCTTAGGC CACATCAAAC GTCGGTACCC ACAGGTTGTG 
ACCCGCCTTA TTCATCTGCT AAGCCAGAAA ATTCTAGGCA ATCTGCAGCA GTTGCAAGGA 
CCCTTTCCAG GCTCCGGGCT CAGTGTTCCG CAGCACTCGG AACTGACCAA CCCAGCCAGC 
AATCTGTCTA CTGTAGCTAT CCTCCCTGTG TGTGCTGAGG TGCCCATGAT GGCCTTTACT 
CTGGAACTGC AGCATGCTCT GCAAGCTATT GGTCCCACGC TCCTCCTTAA CAGTGACGTC 
ATCCGGGCAC TCCTGGGGGC TTCAGCACTG GATAGCATTC AAGAATTCCG GCTGTCAGGG 
TGGTTGGCCC AGCAGGAAGA TGCCCATCGC ATTGTGCTCT ACCAAACTGA CACATCCCTG 
ACGCCTTGGA CCGTCCGTTG TCTGCGCCAG GCCGACTGCA TCCTCATTGT GGGCCTGGGG 
GACCAGGAAC CCACTGTTGG CCAGCTGGAG CAAATGTTAG AGAACACTGC TGTCCGTGCC 
TTGAAGCAAC TGGTTTTGCT GCACCGGGAG GAAGGCCCTG GTCCCACGCG CACTGTAGAG 
TGGCTCAACA TGCGCAGCTG GTGCTCAGGG CACCTGCACC TGCGCTGTCC TCGCCGCCTC 
TTCTCACGGC GCAGCCCAGC GAAACTTCAT GAGCTGTATG AGAAGGTTTT CTCCAGGCGT 
GCAGACCGTC ACAGCGACTT CTCCCGCTTG GCACGAGTAC TCACAGGAAA CACTATTGCT 
CTGGTGCTGG GTGGGGGCGG AGCCAGAGGC TGCTCGCATA TTGGGGTGCT GAAGGCATTA 
GAGGAGGCAG GAGTCCCAGT CGACCTTGTG GGAGGCACAT CCATAGGTTC CTTCATTGGG 
GCCTTGTATG CCGAGGAACG CAGCGCCAGC CGAACTAAAC AACGAGCCCG GGAGTGGGCC 
AAGAGCATGA CTTCTGTACT GGAGCCTGTA TTGGACCTCA CATATCCTGT CACCTCCATG 
TTTACTGGCT CAGCCTTTAA CAGAAGTATC CACCGTGTCT TCCAGGATAA GCAGATTGAG 
GACCTGTGGC TGCCTTACTT CAATGTGACC ACAGACATCA CTGCCTCTGC CATGCGCGTC 
CACAAAGATG GCTCCCTGTG GCGGTATGTA CGTGCCAGCA TGACGCTCTC GGGCTACCTA 
CCCCCGCTGT GCGACCCAAA GGATGGGCAC CTGCTCATGG ATGGTGGCTA CATCAACAAC 
CTGCCAGCGG ATATTGCCCG AAGCATGGGA GCCAAAACGG TCATTGCCAT CGACGTTGGA 
AGCCAGGATG AGACAGATCT CAGCACCTAT GGGGACAGCC TGTCTGGCTG GTGGTTGCTG 
TGGAAAAGGC TAAACCCCTG GGCAGACAAG GTAAAGGTTC CAGACATGGC TGAGATCCAG 
TCCCGCTTGG CGTACGTGTC CTGTGTGCGG CAGCTAGAAG TCGTCAAGTC CAGCTCCTAT 
TGCGAGTACC TTCGTCCATC CATTGACTGC TTCAAGACCA TGGACTTTGG AAAGTTTGAC 
CAGATCTATG ATGTGGGTTA CCAGTATGGG AAGGCTGTCT TTGGAGGCTG GACCCGTGGT 
GAAGTCATTG AGAAAATGCT CACAGACCGG AGATCTACAG ACCTTAATGA GAGTCGCCGT 
GCAGATATAC TTGCCTTCCC AAGTTCTGGG TTCACTGACT TGGCTGAGAT TGTGTCCCGG 
ATCGAGCCAC CAACAAGCTA CGTCTCTGAT GGCTGTGCTG ATGGGGAGGA GTCGGATTGC 
TTGACAGAGT ATGAGGAAGA TGCAGGACCA GACTGCTCAA GAGATGAAGG GGGCTCCCCT 
GAGGGTGCCA GCCCTAGCAC TGCCTCAGAG GTGGAAGAAG AGAAGTCCAC ACTCCGGCAA 
CGACGCTTTC TGCCTCAGGA GACTCCCAGC TCAGTCGCAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508826.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508889.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu40426
Clone ID Related Accession (Same CDS sequence)	XM_006508826.3 , XM_006508829.1 , XM_006508827.4 , XM_006508829.2 , XM_006508827.3 , NM_001359122.1 , XM_006508828.1
Accession Version	XM_006508826.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	neuropathy target esterase isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508826.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGACCCCC 
ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG 
TCAGCCACAG GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT 
GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC 
AGCAGAGTCT TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG 
GATGTGAGCC TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC 
AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG 
GTGCTCACTG GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG 
CGGATCTCCA AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT 
GCAGCTCACA CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT 
GACTGGACAG CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC 
ACCTACATTG TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG 
GAGCTAGTTG GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG 
CAGCCACGAG CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG 
GGCACCTTAG GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG 
CTAAGCCAGA AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG 
CTCAGTGTTC CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT 
ATCCTCCCTG TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT 
CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG 
GCTTCAGCAC TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA 
GATGCCCATC GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT 
TGTCTGCGCC AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT 
GGCCAGCTGG AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG 
CTGCACCGGG AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC 
TGGTGCTCAG GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA 
GCGAAACTTC ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC 
TTCTCCCGCT TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC 
GGAGCCAGAG GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA 
GTCGACCTTG TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA 
CGCAGCGCCA GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA 
CTGGAGCCTG TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT 
AACAGAAGTA TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC 
TTCAATGTGA CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG 
TGGCGGTATG TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA 
AAGGATGGGC ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC 
CGAAGCATGG GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT 
CTCAGCACCT ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC 
TGGGCAGACA AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG 
TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA 
TCCATTGACT GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT 
TACCAGTATG GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG 
CTCACAGACC GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC 
CCAAGTTCTG GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC 
TACGTCTCTG ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA 
GATGCAGGAC CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC 
ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG 
GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508889.1
CDS	258..4307
Translation	MEESLQPKDCRLQDLLPSPKQSMEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508826.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508826.3

ORF Insert Sequence:

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGACCCCC 
ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG 
TCAGCCACAG GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT 
GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC 
AGCAGAGTCT TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG 
GATGTGAGCC TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC 
AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG 
GTGCTCACTG GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG 
CGGATCTCCA AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT 
GCAGCTCACA CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT 
GACTGGACAG CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC 
ACCTACATTG TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG 
GAGCTAGTTG GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG 
CAGCCACGAG CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG 
GGCACCTTAG GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG 
CTAAGCCAGA AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG 
CTCAGTGTTC CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT 
ATCCTCCCTG TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT 
CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG 
GCTTCAGCAC TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA 
GATGCCCATC GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT 
TGTCTGCGCC AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT 
GGCCAGCTGG AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG 
CTGCACCGGG AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC 
TGGTGCTCAG GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA 
GCGAAACTTC ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC 
TTCTCCCGCT TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC 
GGAGCCAGAG GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA 
GTCGACCTTG TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA 
CGCAGCGCCA GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA 
CTGGAGCCTG TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT 
AACAGAAGTA TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC 
TTCAATGTGA CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG 
TGGCGGTATG TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA 
AAGGATGGGC ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC 
CGAAGCATGG GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT 
CTCAGCACCT ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC 
TGGGCAGACA AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG 
TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA 
TCCATTGACT GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT 
TACCAGTATG GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG 
CTCACAGACC GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC 
CCAAGTTCTG GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC 
TACGTCTCTG ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA 
GATGCAGGAC CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC 
ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG 
GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508829.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508892.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X8, mRNA.

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CloneID	OMu40426
Clone ID Related Accession (Same CDS sequence)	XM_006508826.3 , XM_006508829.1 , XM_006508827.4 , XM_006508829.2 , XM_006508827.3 , NM_001359122.1 , XM_006508828.1
Accession Version	XM_006508829.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGACCCCC 
ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG 
TCAGCCACAG GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT 
GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC 
AGCAGAGTCT TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG 
GATGTGAGCC TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC 
AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG 
GTGCTCACTG GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG 
CGGATCTCCA AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT 
GCAGCTCACA CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT 
GACTGGACAG CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC 
ACCTACATTG TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG 
GAGCTAGTTG GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG 
CAGCCACGAG CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG 
GGCACCTTAG GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG 
CTAAGCCAGA AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG 
CTCAGTGTTC CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT 
ATCCTCCCTG TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT 
CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG 
GCTTCAGCAC TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA 
GATGCCCATC GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT 
TGTCTGCGCC AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT 
GGCCAGCTGG AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG 
CTGCACCGGG AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC 
TGGTGCTCAG GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA 
GCGAAACTTC ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC 
TTCTCCCGCT TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC 
GGAGCCAGAG GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA 
GTCGACCTTG TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA 
CGCAGCGCCA GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA 
CTGGAGCCTG TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT 
AACAGAAGTA TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC 
TTCAATGTGA CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG 
TGGCGGTATG TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA 
AAGGATGGGC ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC 
CGAAGCATGG GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT 
CTCAGCACCT ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC 
TGGGCAGACA AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG 
TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA 
TCCATTGACT GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT 
TACCAGTATG GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG 
CTCACAGACC GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC 
CCAAGTTCTG GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC 
TACGTCTCTG ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA 
GATGCAGGAC CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC 
ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG 
GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508892.1
CDS	66..4115
Translation	MEESLQPKDCRLQDLLPSPKQSMEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508829.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508829.1

ORF Insert Sequence:

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGACCCCC 
ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG 
TCAGCCACAG GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT 
GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC 
AGCAGAGTCT TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG 
GATGTGAGCC TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC 
AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG 
GTGCTCACTG GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG 
CGGATCTCCA AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT 
GCAGCTCACA CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT 
GACTGGACAG CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC 
ACCTACATTG TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG 
GAGCTAGTTG GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG 
CAGCCACGAG CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG 
GGCACCTTAG GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG 
CTAAGCCAGA AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG 
CTCAGTGTTC CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT 
ATCCTCCCTG TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT 
CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG 
GCTTCAGCAC TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA 
GATGCCCATC GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT 
TGTCTGCGCC AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT 
GGCCAGCTGG AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG 
CTGCACCGGG AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC 
TGGTGCTCAG GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA 
GCGAAACTTC ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC 
TTCTCCCGCT TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC 
GGAGCCAGAG GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA 
GTCGACCTTG TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA 
CGCAGCGCCA GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA 
CTGGAGCCTG TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT 
AACAGAAGTA TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC 
TTCAATGTGA CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG 
TGGCGGTATG TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA 
AAGGATGGGC ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC 
CGAAGCATGG GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT 
CTCAGCACCT ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC 
TGGGCAGACA AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG 
TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA 
TCCATTGACT GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT 
TACCAGTATG GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG 
CTCACAGACC GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC 
CCAAGTTCTG GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC 
TACGTCTCTG ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA 
GATGCAGGAC CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC 
ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG 
GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508827.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508890.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X6, mRNA.

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CloneID	OMu40426
Clone ID Related Accession (Same CDS sequence)	XM_006508826.3 , XM_006508829.1 , XM_006508827.4 , XM_006508829.2 , XM_006508827.3 , NM_001359122.1 , XM_006508828.1
Accession Version	XM_006508827.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508827.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGACCCCC 
ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG 
TCAGCCACAG GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT 
GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC 
AGCAGAGTCT TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG 
GATGTGAGCC TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC 
AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG 
GTGCTCACTG GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG 
CGGATCTCCA AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT 
GCAGCTCACA CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT 
GACTGGACAG CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC 
ACCTACATTG TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG 
GAGCTAGTTG GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG 
CAGCCACGAG CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG 
GGCACCTTAG GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG 
CTAAGCCAGA AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG 
CTCAGTGTTC CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT 
ATCCTCCCTG TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT 
CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG 
GCTTCAGCAC TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA 
GATGCCCATC GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT 
TGTCTGCGCC AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT 
GGCCAGCTGG AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG 
CTGCACCGGG AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC 
TGGTGCTCAG GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA 
GCGAAACTTC ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC 
TTCTCCCGCT TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC 
GGAGCCAGAG GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA 
GTCGACCTTG TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA 
CGCAGCGCCA GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA 
CTGGAGCCTG TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT 
AACAGAAGTA TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC 
TTCAATGTGA CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG 
TGGCGGTATG TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA 
AAGGATGGGC ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC 
CGAAGCATGG GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT 
CTCAGCACCT ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC 
TGGGCAGACA AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG 
TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA 
TCCATTGACT GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT 
TACCAGTATG GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG 
CTCACAGACC GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC 
CCAAGTTCTG GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC 
TACGTCTCTG ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA 
GATGCAGGAC CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC 
ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG 
GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508890.1
CDS	255..4304
Translation	MEESLQPKDCRLQDLLPSPKQSMEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508827.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508827.3

ORF Insert Sequence:

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGACCCCC 
ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG 
TCAGCCACAG GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT 
GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC 
AGCAGAGTCT TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG 
GATGTGAGCC TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC 
AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG 
GTGCTCACTG GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG 
CGGATCTCCA AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT 
GCAGCTCACA CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT 
GACTGGACAG CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC 
ACCTACATTG TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG 
GAGCTAGTTG GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG 
CAGCCACGAG CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG 
GGCACCTTAG GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG 
CTAAGCCAGA AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG 
CTCAGTGTTC CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT 
ATCCTCCCTG TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT 
CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG 
GCTTCAGCAC TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA 
GATGCCCATC GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT 
TGTCTGCGCC AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT 
GGCCAGCTGG AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG 
CTGCACCGGG AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC 
TGGTGCTCAG GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA 
GCGAAACTTC ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC 
TTCTCCCGCT TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC 
GGAGCCAGAG GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA 
GTCGACCTTG TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA 
CGCAGCGCCA GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA 
CTGGAGCCTG TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT 
AACAGAAGTA TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC 
TTCAATGTGA CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG 
TGGCGGTATG TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA 
AAGGATGGGC ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC 
CGAAGCATGG GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT 
CTCAGCACCT ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC 
TGGGCAGACA AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG 
TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA 
TCCATTGACT GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT 
TACCAGTATG GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG 
CTCACAGACC GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC 
CCAAGTTCTG GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC 
TACGTCTCTG ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA 
GATGCAGGAC CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC 
ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG 
GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla6 ( XM_006508828.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508891.1 Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu40426
Clone ID Related Accession (Same CDS sequence)	XM_006508826.3 , XM_006508829.1 , XM_006508827.4 , XM_006508829.2 , XM_006508827.3 , NM_001359122.1 , XM_006508828.1
Accession Version	XM_006508828.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neuropathy target esterase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGACCCCC 
ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG 
TCAGCCACAG GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT 
GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC 
AGCAGAGTCT TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG 
GATGTGAGCC TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC 
AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG 
GTGCTCACTG GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG 
CGGATCTCCA AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT 
GCAGCTCACA CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT 
GACTGGACAG CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC 
ACCTACATTG TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG 
GAGCTAGTTG GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG 
CAGCCACGAG CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG 
GGCACCTTAG GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG 
CTAAGCCAGA AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG 
CTCAGTGTTC CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT 
ATCCTCCCTG TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT 
CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG 
GCTTCAGCAC TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA 
GATGCCCATC GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT 
TGTCTGCGCC AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT 
GGCCAGCTGG AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG 
CTGCACCGGG AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC 
TGGTGCTCAG GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA 
GCGAAACTTC ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC 
TTCTCCCGCT TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC 
GGAGCCAGAG GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA 
GTCGACCTTG TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA 
CGCAGCGCCA GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA 
CTGGAGCCTG TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT 
AACAGAAGTA TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC 
TTCAATGTGA CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG 
TGGCGGTATG TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA 
AAGGATGGGC ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC 
CGAAGCATGG GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT 
CTCAGCACCT ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC 
TGGGCAGACA AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG 
TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA 
TCCATTGACT GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT 
TACCAGTATG GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG 
CTCACAGACC GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC 
CCAAGTTCTG GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC 
TACGTCTCTG ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA 
GATGCAGGAC CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC 
ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG 
GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508891.1
CDS	88..4137
Translation	MEESLQPKDCRLQDLLPSPKQSMEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGPAGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRTPTQELREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSHFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIRALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQMLENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

Target ORF information:

RefSeq Version	XM_006508828.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508828.1

ORF Insert Sequence:

ATGGAGGAGT CACTCCAGCC AAAGGACTGC AGGCTACAAG ACCTGCTACC TAGTCCGAAA 
CAATCGATGG AGGCCCCACT GCAAACCGGA ATGGTGCTGG GCGTGATGAT CGGGGCCGGA 
GTGGCGGTGC TGGTCACCGC CGTGCTCATC CTCTTGGTGG TGCGAAGGCT GCGGGTGCAG 
AAAACTCCAG CCCCGGAGGG TCCCCGGTAT CGGTTCCGGA AGCGGGACAA AGTGCTTTTC 
TATGGCCGGA AGATTATGCG GAAGGTGTCA CAGTCCACTT CTTCCCTGGT GGACACATCA 
GTCTCCACCA CTTCCCGGCC CCGCATGAAG AAGAAACTGA AGATGCTTAA CATTGCCAAG 
AAGATCTTAC GTATCCAAAA GGAGACTCCG ACCCTACAAC GGAAGGAGCC CCCACCTTCA 
GTGCTGGAGG CTGACCTCAC AGAGGGAGAC CTGGCCAATT CCCACCTACC CTCTGAGGTA 
CTCTACATGC TTAAGAATGT TCGGGTGCTA GGTCACTTTG AGAAGCCGCT CTTCCTGGAG 
CTCTGCCGGC ACATGGTCTT CCAGCGTCTC GGGCAGGGGG ACTATGTTTT TCGGCCCGGT 
CAACCAGATG CCAGCATCTA TGTGGTTCAA GATGGGCTGC TGGAGCTCTG CCTACCAGGG 
CCTGATGGGA AGGAGTGTGT GGTGAAGGAG GTGGTTCCTG GAGACAGCGT CAACAGCCTT 
CTGAGCATCC TTGATGTCAT CACGGGTCAC CAGCATCCCC AGCGGACTGT GTCAGCCAGG 
GCTGCACGGG ACTCCACAGT GCTGAGACTC CCAGTAGAAG CCTTCTCTGC TGTCTTCACC 
AAGTACCCTG AGAGTTTGGT GCGAGTGGTA CAGATCATCA TGGTAAGGCT GCAGAGAGTC 
ACCTTCCTGG CGCTTCACAA TTACCTGGGT CTGACCAATG AACTCTTCAG TCATGAGATC 
CAGCCCCTAC GCCTTTTCCC CAGCCCCGGC CTCCCGACCC GAACCAGCCC TGTCCGTGGC 
TCTAAACGGG TAGTCAGCAC CTCAGGTACT GAGGACACCT CAAAGGAGAC CTCTGGCCGG 
CCCCTGGACT CCATTGGAGC TCCTCTGCCT GGACCTGCAG GGGATCCCGT GAAGCCCACA 
TCCTTAGAAG CACCCCCAGC CCCGTTACTG AGCCGCTGCA TCTCCATGCC AGTAGACATC 
TCAGGCTTGC AAGGTGGCCC TCGTTCTGAT TTTGACATGG CCTATGAACG TGGACGGATA 
TCTGTTTCTC TTCAAGAAGA GGCATCTGGG GGACCTCAGA CAGCATCCCC TAGGACCCCC 
ACTCAGGAGC TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG 
TCAGCCACAG GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT 
GAGGCTGCAA AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC 
AGCAGAGTCT TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG 
GATGTGAGCC TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC 
AAGGCTGAGG AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG 
GTGCTCACTG GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG 
CGGATCTCCA AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT 
GCAGCTCACA CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT 
GACTGGACAG CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC 
ACCTACATTG TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG 
GAGCTAGTTG GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG 
CAGCCACGAG CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG 
GGCACCTTAG GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG 
CTAAGCCAGA AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG 
CTCAGTGTTC CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT 
ATCCTCCCTG TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT 
CTGCAAGCTA TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG 
GCTTCAGCAC TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA 
GATGCCCATC GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT 
TGTCTGCGCC AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT 
GGCCAGCTGG AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG 
CTGCACCGGG AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC 
TGGTGCTCAG GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA 
GCGAAACTTC ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC 
TTCTCCCGCT TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC 
GGAGCCAGAG GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA 
GTCGACCTTG TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA 
CGCAGCGCCA GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA 
CTGGAGCCTG TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT 
AACAGAAGTA TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC 
TTCAATGTGA CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG 
TGGCGGTATG TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA 
AAGGATGGGC ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC 
CGAAGCATGG GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT 
CTCAGCACCT ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC 
TGGGCAGACA AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG 
TCCTGTGTGC GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA 
TCCATTGACT GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT 
TACCAGTATG GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG 
CTCACAGACC GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC 
CCAAGTTCTG GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC 
TACGTCTCTG ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA 
GATGCAGGAC CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC 
ACTGCCTCAG AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG 
GAGACTCCCA GCTCAGTCGC AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NTE/PNPLA6 is expressed in mature Schwann cells and is required for glial ensheathment of Remak fibers.
Glia65(5)804-816(2017 May)
McFerrin J,Patton BL,Sunderhaus ER,Kretzschmar D

Expression of Neuropathy Target Esterase in mouse embryonic stem cells during differentiation.
Archives of toxicology84(6)481-91(2010 Jun)
Pamies D,Reig JA,Vilanova E,Sogorb MA

Spongiform pathology in mouse CNS lacking 'neuropathy target esterase' and cellular prion protein.
Neurobiology of disease35(3)433-7(2009 Sep)
Rosenbluth J,Schiff R,Lam P,Nuriel T,Chao MV

Neuropathy target esterase is required for adult vertebrate axon maintenance.
The Journal of neuroscience : the official journal of the Society for Neuroscience29(37)11594-600(2009 Sep)
Read DJ,Li Y,Chao MV,Cavanagh JB,Glynn P

Neuropathy target esterase catalyzes osmoprotective renal synthesis of glycerophosphocholine in response to high NaCl.
Proceedings of the National Academy of Sciences of the United States of America103(41)15260-5(2006 Oct)
Gallazzini M,Ferraris JD,Kunin M,Morris RG,Burg MB

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Pnpla6 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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