Thop1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Thop1
Entrez Gene ID	50492
Full Name	thimet oligopeptidase 1
Synonyms	AI131655,AI327041,EP24.15
General protein information	Preferred Names thimet oligopeptidase 1 Names thimet oligopeptidase endopeptidase 24.15
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 C1\|10 39.72 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_022653.4	NP_073144.3	thimet oligopeptidase

Pathways from BioSystems

Homologies

Bos taurus (cattle)	THOP1	NP_001029163.2
Eremothecium gossypii	AGOS_AGR405C	NP_987071.2
Mus musculus (house mouse)	Thop1	NP_073144.3
Gallus gallus (chicken)	LOC426398	XP_004948943.1
Xenopus tropicalis (tropical clawed frog)	thop1	NP_001072409.1
Homo sapiens (human)	THOP1	NP_003240.1
Canis lupus familiaris (dog)	THOP1	XP_533954.3
Eremothecium gossypii	AGOS_AGR406C	NP_987072.1
Oryza sativa (rice)	Os01g0902200	NP_001045116.1
Rattus norvegicus (Norway rat)	Thop1	NP_742072.2
Danio rerio (zebrafish)	zgc:92139	NP_001002355.1
Arabidopsis thaliana (thale cress)	AT1G67690	NP_564902.4
Pan troglodytes (chimpanzee)	THOP1	XP_003953368.1
	THOP1	XP_001117760.2

Gene Ontology
Bibliography

Related articles in PubMed

Peptidases released by necrotic cells control CD8+ T cell cross-priming.
Gamrekelashvili J, Kapanadze T, Han M, Wissing J, Ma C, Jaensch L, Manns MP, Armstrong T, Jaffee E, White AO, Citrin DE, Korangy F, Greten TF
The Journal of clinical investigation123(11)4755-68(2013 Nov)

Nuclear Thimet oligopeptidase is coexpressed with oestrogen receptor alpha in hypothalamic cells and regulated by oestradiol in female mice.
Cyr NE, Kua LH, Bruce LA, Chadwick JG, Tetel MJ, Wolfson AJ
Journal of neuroendocrinology22(8)936-43(2010 Aug)

Increased expression of the oligopeptidase THOP1 is a neuroprotective response to Abeta toxicity.
Pollio G, Hoozemans JJ, Andersen CA, Roncarati R, Rosi MC, van Haastert ES, Seredenina T, Diamanti D, Gotta S, Fiorentini A, Magnoni L, Raggiaschi R, Rozemuller AJ, Casamenti F, Caricasole A, Terstappen GC
Neurobiology of disease31(1)145-58(2008 Jul)

Characterization of thimet oligopeptidase and neurolysin activities in B16F10-Nex2 tumor cells and their involvement in angiogenesis and tumor growth.
Paschoalin T, Carmona AK, Rodrigues EG, Oliveira V, Monteiro HP, Juliano MA, Juliano L, Travassos LR
Molecular cancer644(2007 Jul)

Tagging genes with cassette-exchange sites.
Cobellis G, Nicolaus G, Iovino M, Romito A, Marra E, Barbarisi M, Sardiello M, Di Giorgio FP, Iovino N, Zollo M, Ballabio A, Cortese R
Nucleic acids research33(4)e44(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the cellular peptidases dipeptidyl peptidase 3 (DPP-3) and thimet oligopeptidase 1 (TOP-1), both of which are present in nonimmunogenic necrotic cells, eliminated proteasomal degradation products and blocked Ag cross-presentation
Title: Peptidases released by necrotic cells control CD8+ T cell cross-priming.

Data show that Thimet oligopeptidase (TOP) is coexpressed with estrogen receptor alpha, and estradiol regulates TOP expression in a brain region-specific manner in female mice.
Title: Nuclear Thimet oligopeptidase is coexpressed with oestrogen receptor alpha in hypothalamic cells and regulated by oestradiol in female mice.

increase in THOP1 expression might be part of a compensatory defense mechanism of the brain against an increased Abeta load.
Title: Increased expression of the oligopeptidase THOP1 is a neuroprotective response to Abeta toxicity.

Melanoma cells secrete thimet oligopeptidase which have an important role in tumor proliferation/angiogenesis in vitro and in vivo.
Title: Characterization of thimet oligopeptidase and neurolysin activities in B16F10-Nex2 tumor cells and their involvement in angiogenesis and tumor growth.

EP24.15 association with lipid rafts on the extracellular surface precedes constitutive release of the peptidase into the extracellular milieu for its action on neuropeptides
Title: Metalloendopeptidase EC3.4.24.15 is constitutively released from the exofacial leaflet of lipid rafts in GT1-7 cells.

The following Thop1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Thop1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13171	NM_022653.4 Latest version!	Mus musculus thimet oligopeptidase 1 (Thop1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$405.30 $579.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Thop1 ( NM_022653.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_073144.3 Mus musculus thimet oligopeptidase 1 (Thop1), mRNA.

Price & Availability↑

CloneID	OMu13171
Clone ID Related Accession (Same CDS sequence)	NM_022653.4
Accession Version	NM_022653.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	thimet oligopeptidase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY774276.1, CF163503.1, AK028609.1 and CN723672.1. On Jun 13, 2009 this sequence version replaced NM_022653.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK028609.1, BC031722.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCCCC CCGCAGCCTG TGCTGGGGAC GTGGTGGATG CAGCATCCCC AGCCTCCACC 
GTGAATCATC TTCGCTGGGA TCTGAGCGCA CAGCAGATCA GGGCTCTAAC CACGCAGCTC 
ATCGAGCAGA CCAAGTGTGT GTACGACCGA GTGGGTGCTC AGAACTTCGA GGATGTGTCG 
TATGAGAGCA CACTGAAGGC ACTGGCTGAC GTGGAGGTCA CCTACACAGT GCAGAGGAAC 
ATTCTCGACT TCCCCCAACA CGTGTCCCCG TGCAAGGACA TCCGTGCAGC CAGCACAGAG 
GCTGACAAGA AGCTCTCGGA GTTTGATGTA GAGATGAGCA TGAGACAGGA CGTGTACCAG 
AGGGTCGTGT GGCTGCAGGA GAAAACCCCG AAAGATTCCC TGAAGCCTGA AGCAGCTCGC 
TACCTGGAGC GGCTCATCAA GCTGGGCCGG AGAAACGGCC TCCACTTACC TCAGGACACA 
CAAGAGAAGA TCAAGAACAT CAAGAAGAGG CTGAGCCTGC TGTGCATCGA CTTCAACAAG 
AACCTCAACG AGGACACCAC CTTCCTGCCC TTCACAAGAG AGGAGCTGGG TGGGCTCCCG 
GAGGACTTTC TGAGCTCTCT GGAGAAGGCA GAGGACGGCA AGCTGAAGGT CACCCTCAAG 
TACCCTCACT ACTTCCCGCT GTTGAAGAAG TGCCATGTGC CGGAGACACG ACGCCTGTTG 
GAGGAGGCCT TCAACTGTCG CTGCAAGGAG GAGAACTGTG CCATTCTGAA GGAGCTAGTG 
TCCCTGCGAG CACAGAAGTC CAGCCTGTTG GGGTTCCACA CACACGCGGA CTACGTCCTG 
GAGATGAACA TGGCCAAGAC CAGTCAAACA GTAGCCACCT TCCTAGATGA GCTGGCACAG 
AAGCTGAAGC CCCTGGGTGA GCAGGAGCGC GCGGTCATCC TGGAGCTGAA GGAGGCAGAG 
TGCGCCAAGC GGGGACTGCC ATTCGACGGG CGCATCCACG CATGGGACAT GCGCTACTAC 
ATGAACCAGG TGGAGGAGAC TCGCTATCGT GTGGACCAGA ACCTGCTGAA GGAGTACTTC 
CCCATGCAGG TGGTCACACG TGGGCTGCTG GCCATCTACC AAGAGCTGCT GGGGCTGACC 
TTCACGCTGG AGGAAGGTGC CGCTGCCTGG CACGAGGACG TGCGGCTCTA CTCAGTGCGG 
GACGCCGCCT CTGGAGAGGA GATCGGCAAG TTCTACCTCG ACCTATACCC CAGGGAAGGG 
AAATATGGCC ATGCAGCCTG CTTTGGCCTG CAGCCTGGCT GCCTACGGCA GGATGGCAGC 
CGGCAGCTGG CCGTCGCAGC CATGGTGGCC AATTTCACGA AGCCCACACC TGACGCGCCA 
TCCCTGCTGC AGCATGATGA GGTGGAGACC TACTTCCACG AGTTCGGGCA CGTCATGCAC 
CAGCTGTGCT CGCAGGCGGA GTTTGCCATG TTCAGTGGGA CCCACGTGGA GCGGGACTTT 
GTGGAGGCGC CGTCCCAGAT GCTGGAGAAC TGGGTGTGGG AGAAGGAGCC CCTGATGCGC 
ATGTCTCAGC ACTACCGCAC GGGCAGCGAG GCCCCTCAGG ACCTCCTGGA GAAGCTCATC 
AAGTCTCGCC AGGCCAATGC AGGTCTCTTC AACCTGCGGC AGATTGTGCT TGCCAAGGTG 
GATCAGGTCC TGCACACACA GACAGACGCA GACCCGGCTG AGGAATATGC CCGGCTCTGC 
CAAGAGATCC TCGGGGTGCC AGCCACACCA GGTACCAACA TGCCAGCCAC TTTCGGCCAC 
CTAGCTGGCG GCTATGATGC TCAGTACTAT GGCTACTTAT GGAGCGAAGT GTACTCGATG 
GACATGTTCC ACACACGCTT CAAGCAAGAG GGTGTGCTTA GCCCCAAGGT TGGCATGGAC 
TACAGGACCA GCATCCTGAG GCCAGGGGGC TCTGAGGACG CCAGCGCCAT GCTGAAGCAG 
TTCCTGGGCC GCGACCCCAA GCAGGACGCC TTCCTCCTGA GCAAGGGTCT GCAGGTGGAG 
GGCAGCGAGG CGCCTGCGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073144.3
CDS	181..2244
Translation	MKPPAACAGDVVDAASPASTVNHLRWDLSAQQIRALTTQLIEQTKCVYDRVGAQNFEDVSYESTLKALADVEVTYTVQRNILDFPQHVSPCKDIRAASTEADKKLSEFDVEMSMRQDVYQRVVWLQEKTPKDSLKPEAARYLERLIKLGRRNGLHLPQDTQEKIKNIKKRLSLLCIDFNKNLNEDTTFLPFTREELGGLPEDFLSSLEKAEDGKLKVTLKYPHYFPLLKKCHVPETRRLLEEAFNCRCKEENCAILKELVSLRAQKSSLLGFHTHADYVLEMNMAKTSQTVATFLDELAQKLKPLGEQERAVILELKEAECAKRGLPFDGRIHAWDMRYYMNQVEETRYRVDQNLLKEYFPMQVVTRGLLAIYQELLGLTFTLEEGAAAWHEDVRLYSVRDAASGEEIGKFYLDLYPREGKYGHAACFGLQPGCLRQDGSRQLAVAAMVANFTKPTPDAPSLLQHDEVETYFHEFGHVMHQLCSQAEFAMFSGTHVERDFVEAPSQMLENWVWEKEPLMRMSQHYRTGSEAPQDLLEKLIKSRQANAGLFNLRQIVLAKVDQVLHTQTDADPAEEYARLCQEILGVPATPGTNMPATFGHLAGGYDAQYYGYLWSEVYSMDMFHTRFKQEGVLSPKVGMDYRTSILRPGGSEDASAMLKQFLGRDPKQDAFLLSKGLQVEGSEAPAC

Target ORF information:

RefSeq Version	NM_022653.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus thimet oligopeptidase 1 (Thop1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022653.4

ORF Insert Sequence:

ATGAAGCCCC CCGCAGCCTG TGCTGGGGAC GTGGTGGATG CAGCATCCCC AGCCTCCACC 
GTGAATCATC TTCGCTGGGA TCTGAGCGCA CAGCAGATCA GGGCTCTAAC CACGCAGCTC 
ATCGAGCAGA CCAAGTGTGT GTACGACCGA GTGGGTGCTC AGAACTTCGA GGATGTGTCG 
TATGAGAGCA CACTGAAGGC ACTGGCTGAC GTGGAGGTCA CCTACACAGT GCAGAGGAAC 
ATTCTCGACT TCCCCCAACA CGTGTCCCCG TGCAAGGACA TCCGTGCAGC CAGCACAGAG 
GCTGACAAGA AGCTCTCGGA GTTTGATGTA GAGATGAGCA TGAGACAGGA CGTGTACCAG 
AGGGTCGTGT GGCTGCAGGA GAAAACCCCG AAAGATTCCC TGAAGCCTGA AGCAGCTCGC 
TACCTGGAGC GGCTCATCAA GCTGGGCCGG AGAAACGGCC TCCACTTACC TCAGGACACA 
CAAGAGAAGA TCAAGAACAT CAAGAAGAGG CTGAGCCTGC TGTGCATCGA CTTCAACAAG 
AACCTCAACG AGGACACCAC CTTCCTGCCC TTCACAAGAG AGGAGCTGGG TGGGCTCCCG 
GAGGACTTTC TGAGCTCTCT GGAGAAGGCA GAGGACGGCA AGCTGAAGGT CACCCTCAAG 
TACCCTCACT ACTTCCCGCT GTTGAAGAAG TGCCATGTGC CGGAGACACG ACGCCTGTTG 
GAGGAGGCCT TCAACTGTCG CTGCAAGGAG GAGAACTGTG CCATTCTGAA GGAGCTAGTG 
TCCCTGCGAG CACAGAAGTC CAGCCTGTTG GGGTTCCACA CACACGCGGA CTACGTCCTG 
GAGATGAACA TGGCCAAGAC CAGTCAAACA GTAGCCACCT TCCTAGATGA GCTGGCACAG 
AAGCTGAAGC CCCTGGGTGA GCAGGAGCGC GCGGTCATCC TGGAGCTGAA GGAGGCAGAG 
TGCGCCAAGC GGGGACTGCC ATTCGACGGG CGCATCCACG CATGGGACAT GCGCTACTAC 
ATGAACCAGG TGGAGGAGAC TCGCTATCGT GTGGACCAGA ACCTGCTGAA GGAGTACTTC 
CCCATGCAGG TGGTCACACG TGGGCTGCTG GCCATCTACC AAGAGCTGCT GGGGCTGACC 
TTCACGCTGG AGGAAGGTGC CGCTGCCTGG CACGAGGACG TGCGGCTCTA CTCAGTGCGG 
GACGCCGCCT CTGGAGAGGA GATCGGCAAG TTCTACCTCG ACCTATACCC CAGGGAAGGG 
AAATATGGCC ATGCAGCCTG CTTTGGCCTG CAGCCTGGCT GCCTACGGCA GGATGGCAGC 
CGGCAGCTGG CCGTCGCAGC CATGGTGGCC AATTTCACGA AGCCCACACC TGACGCGCCA 
TCCCTGCTGC AGCATGATGA GGTGGAGACC TACTTCCACG AGTTCGGGCA CGTCATGCAC 
CAGCTGTGCT CGCAGGCGGA GTTTGCCATG TTCAGTGGGA CCCACGTGGA GCGGGACTTT 
GTGGAGGCGC CGTCCCAGAT GCTGGAGAAC TGGGTGTGGG AGAAGGAGCC CCTGATGCGC 
ATGTCTCAGC ACTACCGCAC GGGCAGCGAG GCCCCTCAGG ACCTCCTGGA GAAGCTCATC 
AAGTCTCGCC AGGCCAATGC AGGTCTCTTC AACCTGCGGC AGATTGTGCT TGCCAAGGTG 
GATCAGGTCC TGCACACACA GACAGACGCA GACCCGGCTG AGGAATATGC CCGGCTCTGC 
CAAGAGATCC TCGGGGTGCC AGCCACACCA GGTACCAACA TGCCAGCCAC TTTCGGCCAC 
CTAGCTGGCG GCTATGATGC TCAGTACTAT GGCTACTTAT GGAGCGAAGT GTACTCGATG 
GACATGTTCC ACACACGCTT CAAGCAAGAG GGTGTGCTTA GCCCCAAGGT TGGCATGGAC 
TACAGGACCA GCATCCTGAG GCCAGGGGGC TCTGAGGACG CCAGCGCCAT GCTGAAGCAG 
TTCCTGGGCC GCGACCCCAA GCAGGACGCC TTCCTCCTGA GCAAGGGTCT GCAGGTGGAG 
GGCAGCGAGG CGCCTGCGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Peptidases released by necrotic cells control CD8+ T cell cross-priming.
The Journal of clinical investigation123(11)4755-68(2013 Nov)
Gamrekelashvili J,Kapanadze T,Han M,Wissing J,Ma C,Jaensch L,Manns MP,Armstrong T,Jaffee E,White AO,Citrin DE,Korangy F,Greten TF

Nuclear Thimet oligopeptidase is coexpressed with oestrogen receptor alpha in hypothalamic cells and regulated by oestradiol in female mice.
Journal of neuroendocrinology22(8)936-43(2010 Aug)
Cyr NE,Kua LH,Bruce LA,Chadwick JG,Tetel MJ,Wolfson AJ

Increased expression of the oligopeptidase THOP1 is a neuroprotective response to Abeta toxicity.
Neurobiology of disease31(1)145-58(2008 Jul)
Pollio G,Hoozemans JJ,Andersen CA,Roncarati R,Rosi MC,van Haastert ES,Seredenina T,Diamanti D,Gotta S,Fiorentini A,Magnoni L,Raggiaschi R,Rozemuller AJ,Casamenti F,Caricasole A,Terstappen GC

Characterization of thimet oligopeptidase and neurolysin activities in B16F10-Nex2 tumor cells and their involvement in angiogenesis and tumor growth.
Molecular cancer644(2007 Jul)
Paschoalin T,Carmona AK,Rodrigues EG,Oliveira V,Monteiro HP,Juliano MA,Juliano L,Travassos LR

Tagging genes with cassette-exchange sites.
Nucleic acids research33(4)e44(2005 Mar)
Cobellis G,Nicolaus G,Iovino M,Romito A,Marra E,Barbarisi M,Sardiello M,Di Giorgio FP,Iovino N,Zollo M,Ballabio A,Cortese R

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Thop1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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