Gfral cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gfral
Entrez Gene ID	404194
Full Name	GDNF family receptor alpha like
Synonyms	AY457637,Gral
General protein information	Preferred Names GDNF family receptor alpha like Names GDNF family receptor alpha-like GDNF receptor alpha-like
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 D

mRNA and Protein(s)

mRNA	Protein	Name
NM_205844.3	NP_995316.2	GDNF family receptor alpha-like precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GFRAL	XP_002697218.1
Rattus norvegicus (Norway rat)	Gfral	NP_001178927.1
Homo sapiens (human)	GFRAL	NP_997293.2
Gallus gallus (chicken)	GFRAL	XP_419904.4
	GFRAL	XP_001104929.1
Canis lupus familiaris (dog)	GFRAL	XP_538972.3
Mus musculus (house mouse)	Gfral	NP_995316.2
Xenopus tropicalis (tropical clawed frog)	gfral	XP_002934908.2
Pan troglodytes (chimpanzee)	GFRAL	XP_001157000.1

Gene Ontology
Bibliography

Related articles in PubMed

GFRAL is the receptor for GDF15 and is required for the anti-obesity effects of the ligand.
Yang L, Chang CC, Sun Z, Madsen D, Zhu H, Padkj?r SB, Wu X, Huang T, Hultman K, Paulsen SJ, Wang J, Bugge A, Frantzen JB, N?rgaard P, Jeppesen JF, Yang Z, Secher A, Chen H, Li X, John LM, Shan B, He Z, Gao X, Su J, Hansen KT, Yang W, J?rgensen SB
Nature medicine23(10)1158-1166(2017 Oct)

The metabolic effects of GDF15 are mediated by the orphan receptor GFRAL.
Emmerson PJ, Wang F, Du Y, Liu Q, Pickard RT, Gonciarz MD, Coskun T, Hamang MJ, Sindelar DK, Ballman KK, Foltz LA, Muppidi A, Alsina-Fernandez J, Barnard GC, Tang JX, Liu X, Mao X, Siegel R, Sloan JH, Mitchell PJ, Zhang BB, Gimeno RE, Shan B, Wu X
Nature medicine23(10)1215-1219(2017 Oct)

GFRAL is the receptor for GDF15 and the ligand promotes weight loss in mice and nonhuman primates.
Mullican SE, Lin-Schmidt X, Chin CN, Chavez JA, Furman JL, Armstrong AA, Beck SC, South VJ, Dinh TQ, Cash-Mason TD, Cavanaugh CR, Nelson S, Huang C, Hunter MJ, Rangwala SM
Nature medicine23(10)1150-1157(2017 Oct)

Non-homeostatic body weight regulation through a brainstem-restricted receptor for GDF15.
Hsu JY, Crawley S, Chen M, Ayupova DA, Lindhout DA, Higbee J, Kutach A, Joo W, Gao Z, Fu D, To C, Mondal K, Li B, Kekatpure A, Wang M, Laird T, Horner G, Chan J, McEntee M, Lopez M, Lakshminarasimhan D, White A, Wang SP, Yao J, Yie J, Matern H, Solloway M, Haldankar R, Parsons T, Tang J, Shen WD, Alice Chen Y, Tian H, Allan BB
Nature550(7675)255-259(2017 Oct)

A mouse knockout library for secreted and transmembrane proteins.
Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M, Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ
Nature biotechnology28(7)749-55(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gfral gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gfral cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu09665	NM_205844.3 Latest version!	Mus musculus GDNF family receptor alpha like (Gfral), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gfral ( NM_205844.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_995316.2 Mus musculus GDNF family receptor alpha like (Gfral), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09665
Clone ID Related Accession (Same CDS sequence)	NM_205844.3
Accession Version	NM_205844.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	GDNF family receptor alpha-like precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC079244.29. On Aug 22, 2006 this sequence version replaced NM_205844.2. ##Evidence-Data-START## Transcript exon combination :: AY457637.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01164135 [ECO:0000350] ##Evidence-Data-END##

ATGCTAGTGT TCATTTTCCT GGCTGTTACG TTAAGCTCAG AAAATGAATC CTCTTCCCAA 
ACAAATGATT GTGCACATTT AATACAGAAA TGCTTGATTG ATGCAAATGG CTGTGAGCAG 
TCATGGAGAT CAATGGAAGA CACCTGCCTT ACTCCAGGTG ACTCCTGCAA GATAAATAAT 
TCACTACATT GTAACCTGAG TATCCAGGCT TTGGTGGAAA AAAATTTCCA ATTTAAAGAG 
TGTCTTTGTA TGGATGACCT CCACTGTACA GTAAACAAAC TTTTTGGAAA AAAGTGCACC 
AATAAGACAG ATAACATGGA AAAGGACAAT AAAGATAAAT GGAATCTAAC TACTACTCCT 
TTCTATCATG GATTCAAACA GATGCAGTCT TGTTTGGAGG TGACAGAGGC GTGTGTAGGG 
GATGTGGTTT GTAATGCACA GTTGGCCCTT TACCTTAAAG CATGCTCAGC AAATGGAAAT 
CTGTGTGATG TGAAACACTG CCAAGCAGCC ATACGGTTCT TCTATCAAAA TATGCCTTTT 
AACACTGCCC AGATGTTGGC TTTTTGTGAC TGTGCTCAAT CTGATATACC CTGTCAGCAA 
TCCAAAGAAA CTCTTCACAG CAAGCCATGT GCACTGAATA TAGTTCCACC CCCCACTTGC 
CTCAGTGTAA TTCACACTTG CCGAAATGAT GAATTATGCA GGACACACTA CCGAACATTC 
CAGACAGAAT GCTGGCCCCA CATAACTGGG AAGTGCCATG AAGATGAGAC CTGCATTAGC 
ATGTTAGGCA AGCAAGACCT TACTTGTTCT GGGAGTGAGA GCTGCAGGGC TGCCTTCCTA 
GGAACCTTTG GGACAGTCCT GCAAGTACCC TGTGCTTGCA GGGGCGTTAC ACAGGCTGAA 
GAACACGTGT GCATGATTTT CCAGCACATG CTTCATAGCA AATCGTGTTT CAATTACCCA 
ACTCCTAATG TCAAAGACAT TTCCTCATAT GAAAAAAAGA ATTCAAAAGA AATTACTCTG 
ACTGGATTCA ATTCTTTCTT CAATGGAGAA CTACTCTATG TTGTTGTGTG CATGGCAGTT 
ACCTGTGGAA TTCTTTTCTT GGTGATGCTC AAGTTAAGGA TACAAAGTGA AAAAAGAGAT 
CCCTCATCCA TCGAAATAGC TGGAGGTGTC ATCATTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995316.2
CDS	196..1377
Translation	MLVFIFLAVTLSSENESSSQTNDCAHLIQKCLIDANGCEQSWRSMEDTCLTPGDSCKINNSLHCNLSIQALVEKNFQFKECLCMDDLHCTVNKLFGKKCTNKTDNMEKDNKDKWNLTTTPFYHGFKQMQSCLEVTEACVGDVVCNAQLALYLKACSANGNLCDVKHCQAAIRFFYQNMPFNTAQMLAFCDCAQSDIPCQQSKETLHSKPCALNIVPPPTCLSVIHTCRNDELCRTHYRTFQTECWPHITGKCHEDETCISMLGKQDLTCSGSESCRAAFLGTFGTVLQVPCACRGVTQAEEHVCMIFQHMLHSKSCFNYPTPNVKDISSYEKKNSKEITLTGFNSFFNGELLYVVVCMAVTCGILFLVMLKLRIQSEKRDPSSIEIAGGVIIQ

Target ORF information:

RefSeq Version	NM_205844.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus GDNF family receptor alpha like (Gfral), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205844.3

ORF Insert Sequence:

ATGCTAGTGT TCATTTTCCT GGCTGTTACG TTAAGCTCAG AAAATGAATC CTCTTCCCAA 
ACAAATGATT GTGCACATTT AATACAGAAA TGCTTGATTG ATGCAAATGG CTGTGAGCAG 
TCATGGAGAT CAATGGAAGA CACCTGCCTT ACTCCAGGTG ACTCCTGCAA GATAAATAAT 
TCACTACATT GTAACCTGAG TATCCAGGCT TTGGTGGAAA AAAATTTCCA ATTTAAAGAG 
TGTCTTTGTA TGGATGACCT CCACTGTACA GTAAACAAAC TTTTTGGAAA AAAGTGCACC 
AATAAGACAG ATAACATGGA AAAGGACAAT AAAGATAAAT GGAATCTAAC TACTACTCCT 
TTCTATCATG GATTCAAACA GATGCAGTCT TGTTTGGAGG TGACAGAGGC GTGTGTAGGG 
GATGTGGTTT GTAATGCACA GTTGGCCCTT TACCTTAAAG CATGCTCAGC AAATGGAAAT 
CTGTGTGATG TGAAACACTG CCAAGCAGCC ATACGGTTCT TCTATCAAAA TATGCCTTTT 
AACACTGCCC AGATGTTGGC TTTTTGTGAC TGTGCTCAAT CTGATATACC CTGTCAGCAA 
TCCAAAGAAA CTCTTCACAG CAAGCCATGT GCACTGAATA TAGTTCCACC CCCCACTTGC 
CTCAGTGTAA TTCACACTTG CCGAAATGAT GAATTATGCA GGACACACTA CCGAACATTC 
CAGACAGAAT GCTGGCCCCA CATAACTGGG AAGTGCCATG AAGATGAGAC CTGCATTAGC 
ATGTTAGGCA AGCAAGACCT TACTTGTTCT GGGAGTGAGA GCTGCAGGGC TGCCTTCCTA 
GGAACCTTTG GGACAGTCCT GCAAGTACCC TGTGCTTGCA GGGGCGTTAC ACAGGCTGAA 
GAACACGTGT GCATGATTTT CCAGCACATG CTTCATAGCA AATCGTGTTT CAATTACCCA 
ACTCCTAATG TCAAAGACAT TTCCTCATAT GAAAAAAAGA ATTCAAAAGA AATTACTCTG 
ACTGGATTCA ATTCTTTCTT CAATGGAGAA CTACTCTATG TTGTTGTGTG CATGGCAGTT 
ACCTGTGGAA TTCTTTTCTT GGTGATGCTC AAGTTAAGGA TACAAAGTGA AAAAAGAGAT 
CCCTCATCCA TCGAAATAGC TGGAGGTGTC ATCATTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GFRAL is the receptor for GDF15 and is required for the anti-obesity effects of the ligand.
Nature medicine23(10)1158-1166(2017 Oct)
Yang L,Chang CC,Sun Z,Madsen D,Zhu H,Padkj?r SB,Wu X,Huang T,Hultman K,Paulsen SJ,Wang J,Bugge A,Frantzen JB,N?rgaard P,Jeppesen JF,Yang Z,Secher A,Chen H,Li X,John LM,Shan B,He Z,Gao X,Su J,Hansen KT,Yang W,J?rgensen SB

The metabolic effects of GDF15 are mediated by the orphan receptor GFRAL.
Nature medicine23(10)1215-1219(2017 Oct)
Emmerson PJ,Wang F,Du Y,Liu Q,Pickard RT,Gonciarz MD,Coskun T,Hamang MJ,Sindelar DK,Ballman KK,Foltz LA,Muppidi A,Alsina-Fernandez J,Barnard GC,Tang JX,Liu X,Mao X,Siegel R,Sloan JH,Mitchell PJ,Zhang BB,Gimeno RE,Shan B,Wu X

GFRAL is the receptor for GDF15 and the ligand promotes weight loss in mice and nonhuman primates.
Nature medicine23(10)1150-1157(2017 Oct)
Mullican SE,Lin-Schmidt X,Chin CN,Chavez JA,Furman JL,Armstrong AA,Beck SC,South VJ,Dinh TQ,Cash-Mason TD,Cavanaugh CR,Nelson S,Huang C,Hunter MJ,Rangwala SM

Non-homeostatic body weight regulation through a brainstem-restricted receptor for GDF15.
Nature550(7675)255-259(2017 Oct)
Hsu JY,Crawley S,Chen M,Ayupova DA,Lindhout DA,Higbee J,Kutach A,Joo W,Gao Z,Fu D,To C,Mondal K,Li B,Kekatpure A,Wang M,Laird T,Horner G,Chan J,McEntee M,Lopez M,Lakshminarasimhan D,White A,Wang SP,Yao J,Yie J,Matern H,Solloway M,Haldankar R,Parsons T,Tang J,Shen WD,Alice Chen Y,Tian H,Allan BB

A mouse knockout library for secreted and transmembrane proteins.
Nature biotechnology28(7)749-55(2010 Jul)
Tang T,Li L,Tang J,Li Y,Lin WY,Martin F,Grant D,Solloway M,Parker L,Ye W,Forrest W,Ghilardi N,Oravecz T,Platt KA,Rice DS,Hansen GM,Abuin A,Eberhart DE,Godowski P,Holt KH,Peterson A,Zambrowicz BP,de Sauvage FJ

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Gfral cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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