Rnf133 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnf133
Entrez Gene ID	386611
Full Name	ring finger protein 133
Synonyms	Greul2
General protein information	Preferred Names ring finger protein 133 Names E3 ubiquitin-protein ligase RNF133 RING-type E3 ubiquitin transferase RNF133 goliath-related E3 ubiquitin-protein ligase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 A3.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_198251.2	NP_937894.1	E3 ubiquitin-protein ligase RNF133

Pathways from BioSystems

Homologies

	RNF133	XP_001084535.1
Homo sapiens (human)	RNF133	NP_631914.1
Pan troglodytes (chimpanzee)	RNF133	XP_001146813.1
Mus musculus (house mouse)	Rnf133	NP_937894.1
Rattus norvegicus (Norway rat)	Rnf133	NP_001037743.1
Canis lupus familiaris (dog)	RNF133	XP_005628826.1
Bos taurus (cattle)	RNF133	XP_005205723.1

Gene Ontology
Bibliography

Related articles in PubMed

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Mouse RING finger protein Rnf133 is a testis-specific endoplasmic reticulum-associated E3 ubiquitin ligase.
Nian H, Zhang W, Shi H, Zhao Q, Xie Q, Liao S, Zhang Y, Zhang Z, Wang C, Han C
Cell research18(7)800-2(2008 Jul)

The E3 ubiquitin ligase GREUL1 anteriorizes ectoderm during Xenopus development.
Borchers AG, Hufton AL, Eldridge AG, Jackson PK, Harland RM, Baker JC
Developmental biology251(2)395-408(2002 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study identified a potential testis-specific gene named Rnf133.
Title: Mouse RING finger protein Rnf133 is a testis-specific endoplasmic reticulum-associated E3 ubiquitin ligase.

The following Rnf133 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnf133 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08934	NM_198251.2 Latest version!	Mus musculus ring finger protein 133 (Rnf133), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnf133 ( NM_198251.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_937894.1 Mus musculus ring finger protein 133 (Rnf133), mRNA.

Price & Availability↑

CloneID	OMu08934
Clone ID Related Accession (Same CDS sequence)	NM_198251.2
Accession Version	NM_198251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF133
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK028082.1. On Apr 5, 2007 this sequence version replaced NM_198251.1. ##Evidence-Data-START## Transcript exon combination :: AK028082.1, AV045338.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGAATCCTC TTCAGACCAG TACTTGGCAA AACCAAGCTC CATCTTTCTG GCTTCTGAGA 
TTCAGTTTTA TCTGGCTCGT CAGTCAGAAA TGTTGTACAG CCAGTGCAGT TTGGACTGCG 
TACATGAACA TATCATTTCA TGTGGGGAAC CGCATGCTGT CAGAGCTGGG GGAGACCGGG 
GTCTTCGGAA GAAGCTCCAT CTTGAAGAGA GTAGCAGGAG TTGTTGTGCC ACCAGAGGGA 
AAAATTCAAA ATGCTTGTGA TCCCAATACC ACTTTTATCC TGCCACGGAA CAAAGAGCCC 
TGGATCGCAC TCATTGAACG GGGAGGTTGT GCCTTCACAC AGAAAATTAA GGTGGCATCT 
GAGCACGGAG CCAGAGGAGT GATCATCTAT AATTTCCCAG GTACTGGCAA CCAGGTTTTC 
CCCATGTCTC ACCAGGCGTT TGAAGACATC GTTGTAGTTA TGATTGGTAA CATCAAAGCA 
TATTTCACCT TTTATCACAT TCGGAGACTT TGGGTGGCAA GAATTGAGAA CAGGAGATGG 
AAGCGATTAA CAAGAGAGCT CAAGAAAGCT TTTGGGCAGC TGCAAGTTCG AGTATTAAAA 
GAAGGGGATG AAGAAGTTAA TCCAAATGCT GACAGCTGTG TCATCTGCTT TGAAGCCTAT 
AAGCCTAATG AAATAGTTCG GATTCTCACT TGCAAACATT TTTTCCACAA GAATTGCATT 
GACCCCTGGA TTCTAGCCCA CGGCACATGC CCCATGTGCA AATGTGACAT TCTGAAAGCT 
CTGGGGATTC AGATGGACAT TGAGGATGGG ACAGACTCTC TGCAAGTTCT GATGTCAAAT 
GAATTGCCCG GAACCTTGTC ACCCGTGGAA GAAGAGACGA ACTATGAGCT TCCTCCTGCA 
AGAACTTCAA GTAAAGTGAC CCACGTGCAG GAGCACCCTA CTTCTTCTGC GAACGCTGGC 
AGCCAGCCTC CCGAAGCAGA GGAAACCAGT CATCCTTCAC ATGGACAGCA AGTCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937894.1
CDS	249..1268
Translation	MNPLQTSTWQNQAPSFWLLRFSFIWLVSQKCCTASAVWTAYMNISFHVGNRMLSELGETGVFGRSSILKRVAGVVVPPEGKIQNACDPNTTFILPRNKEPWIALIERGGCAFTQKIKVASEHGARGVIIYNFPGTGNQVFPMSHQAFEDIVVVMIGNIKAYFTFYHIRRLWVARIENRRWKRLTRELKKAFGQLQVRVLKEGDEEVNPNADSCVICFEAYKPNEIVRILTCKHFFHKNCIDPWILAHGTCPMCKCDILKALGIQMDIEDGTDSLQVLMSNELPGTLSPVEEETNYELPPARTSSKVTHVQEHPTSSANAGSQPPEAEETSHPSHGQQVL

Target ORF information:

RefSeq Version	NM_198251.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 133 (Rnf133), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198251.2

ORF Insert Sequence:

ATGAATCCTC TTCAGACCAG TACTTGGCAA AACCAAGCTC CATCTTTCTG GCTTCTGAGA 
TTCAGTTTTA TCTGGCTCGT CAGTCAGAAA TGTTGTACAG CCAGTGCAGT TTGGACTGCG 
TACATGAACA TATCATTTCA TGTGGGGAAC CGCATGCTGT CAGAGCTGGG GGAGACCGGG 
GTCTTCGGAA GAAGCTCCAT CTTGAAGAGA GTAGCAGGAG TTGTTGTGCC ACCAGAGGGA 
AAAATTCAAA ATGCTTGTGA TCCCAATACC ACTTTTATCC TGCCACGGAA CAAAGAGCCC 
TGGATCGCAC TCATTGAACG GGGAGGTTGT GCCTTCACAC AGAAAATTAA GGTGGCATCT 
GAGCACGGAG CCAGAGGAGT GATCATCTAT AATTTCCCAG GTACTGGCAA CCAGGTTTTC 
CCCATGTCTC ACCAGGCGTT TGAAGACATC GTTGTAGTTA TGATTGGTAA CATCAAAGCA 
TATTTCACCT TTTATCACAT TCGGAGACTT TGGGTGGCAA GAATTGAGAA CAGGAGATGG 
AAGCGATTAA CAAGAGAGCT CAAGAAAGCT TTTGGGCAGC TGCAAGTTCG AGTATTAAAA 
GAAGGGGATG AAGAAGTTAA TCCAAATGCT GACAGCTGTG TCATCTGCTT TGAAGCCTAT 
AAGCCTAATG AAATAGTTCG GATTCTCACT TGCAAACATT TTTTCCACAA GAATTGCATT 
GACCCCTGGA TTCTAGCCCA CGGCACATGC CCCATGTGCA AATGTGACAT TCTGAAAGCT 
CTGGGGATTC AGATGGACAT TGAGGATGGG ACAGACTCTC TGCAAGTTCT GATGTCAAAT 
GAATTGCCCG GAACCTTGTC ACCCGTGGAA GAAGAGACGA ACTATGAGCT TCCTCCTGCA 
AGAACTTCAA GTAAAGTGAC CCACGTGCAG GAGCACCCTA CTTCTTCTGC GAACGCTGGC 
AGCCAGCCTC CCGAAGCAGA GGAAACCAGT CATCCTTCAC ATGGACAGCA AGTCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Mouse RING finger protein Rnf133 is a testis-specific endoplasmic reticulum-associated E3 ubiquitin ligase.
Cell research18(7)800-2(2008 Jul)
Nian H,Zhang W,Shi H,Zhao Q,Xie Q,Liao S,Zhang Y,Zhang Z,Wang C,Han C

The E3 ubiquitin ligase GREUL1 anteriorizes ectoderm during Xenopus development.
Developmental biology251(2)395-408(2002 Nov)
Borchers AG,Hufton AL,Eldridge AG,Jackson PK,Harland RM,Baker JC

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Rnf133 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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