Rxfp1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rxfp1
Entrez Gene ID	381489
Full Name	relaxin/insulin-like family peptide receptor 1
Synonyms	Gm1018,Lgr7
General protein information	Preferred Names relaxin/insulin-like family peptide receptor 1 Names relaxin receptor 1 leucine-rich repeat-containing 7 leucine-rich repeat-containing G-protein coupled receptor 7 relaxin family peptide receptor 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 E3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006501629.2	XP_006501692.1	relaxin receptor 1 isoform X1
XM_030252661.1	XP_030108521.1	relaxin receptor 1 isoform X2
XM_006501630.2	XP_006501693.1	relaxin receptor 1 isoform X2
NM_212452.2	NP_997617.1	relaxin receptor 1 precursor
NM_212452.1	NP_997617.1	relaxin receptor 1 precursor
XM_030252662.1	XP_030108522.1	relaxin receptor 1 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RXFP1	XP_001144181.2
Bos taurus (cattle)	RXFP1	XP_002694461.1
Mus musculus (house mouse)	Rxfp1	NP_997617.1
Gallus gallus (chicken)	RXFP1	XP_420385.2
Danio rerio (zebrafish)	rxfp1	NP_001177863.1
Homo sapiens (human)	RXFP1	NP_001240656.1
Canis lupus familiaris (dog)	RXFP1	XP_005629433.1
	RXFP1	XP_001096574.1
Anopheles gambiae (African malaria mosquito)	GPRGPH	XP_321252.4
Rattus norvegicus (Norway rat)	Rxfp1	NP_958820.1
Drosophila melanogaster (fruit fly)	CG34411	NP_001096966.2
Xenopus tropicalis (tropical clawed frog)	rxfp1	XP_002934794.2

Gene Ontology
Bibliography

Related articles in PubMed

Relaxin induces up-regulation of ADAM10 metalloprotease in RXFP1-expressing cells by PI3K/AKT signaling.
Boccalini G, Sassoli C, Bani D, Nistri S
Molecular and cellular endocrinology(2017 Nov)

Relaxin family peptides: structure-activity relationship studies.
Patil NA, Rosengren KJ, Separovic F, Wade JD, Bathgate RAD, Hossain MA
British journal of pharmacology174(10)950-961(2017 May)

Relaxin deficiency results in increased expression of angiogenesis- and remodelling-related genes in the uterus of early pregnant mice but does not affect endometrial angiogenesis prior to implantation.
Marshall SA, Ng L, Unemori EN, Girling JE, Parry LJ
Reproductive biology and endocrinology : RB&E1411(2016 Mar)

Relaxin Affects Smooth Muscle Biophysical Properties and Mechanical Activity of the Female Mouse Colon.
Squecco R, Garella R, Idrizaj E, Nistri S, Francini F, Baccari MC
Endocrinology156(12)4398-410(2015 Dec)

In a Class of Their Own - RXFP1 and RXFP2 are Unique Members of the LGR Family.
Petrie EJ, Lagaida S, Sethi A, Bathgate RA, Gooley PR
Frontiers in endocrinology6137(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Colonic tissue expresses RXFP1 in smooth muscle cells of the muscular wall and in neurons of the myenteric plexus.
Title: Relaxin Affects Smooth Muscle Biophysical Properties and Mechanical Activity of the Female Mouse Colon.

No differences were found in RXFP1 expression related to aging.
Title: Differential effects of relaxin deficiency on vascular aging in arteries of male mice.

relaxin/RXFP1 signaling in smooth muscle cells is important for normal collagen turnover and relaxation of the pubic symphysis during pregnancy
Title: Conditional deletion of the relaxin receptor gene in cells of smooth muscle lineage affects lower reproductive tract in pregnant mice.

Rxfp1, Rxfp2, and Nr3c1 mRNAs were expressed on the developing maxilla and mandible, Meckel's cartilage, tongue, and tooth primordia between mouse embryonic days 13.5-18.5.
Title: Relaxin receptors 1 and 2 and nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) mRNAs are expressed in oral components of developing mice.

maternal exposure to simazine causes disturbed relaxin signaling pathway and testicular dysfunction
Title: Disturbed relaxin signaling pathway and testicular dysfunction in mouse offspring upon maternal exposure to simazine.

The following Rxfp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rxfp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu32020	XM_006501630.2 Latest version!	Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu32019	XM_006501629.2 Latest version!	Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu10195	NM_212452.2 Latest version!	Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$447.30 $639.00
OMu32020	XM_030252661.1 Latest version!	Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu88247	XM_030252662.1 Latest version!	Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu10195	NM_212452.1	Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$447.30 $639.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rxfp1 ( XM_006501630.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501693.1 Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu32020
Clone ID Related Accession (Same CDS sequence)	XM_030252661.1 , XM_006501630.2
Accession Version	XM_006501630.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	relaxin receptor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006501630.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCCC TGCAGAGGAA CTTCATAAGA ACACTCCCTC CCAACGGCTT CAGGAAGTAC 
CATGAGCTGC AGAAGCTGTG CCTGCAAAAC AATAGGATTC ACTCCGTGAG CGTCTCTGCC 
TTCAGAGGAC TGCGCAGCCT CACTAAACTG TACCTCAGTC ATAACAGAAT AACCTTCCTA 
AAGCCAGGAG TTTTTGAAGA TCTCCACAGA CTGGAATGGC TGATAATTGA AGACAATCAC 
CTCAGTCGAA TTTCCCCACT CACATTTTAC GGACTAAATT CTCTTATTCT CTTAGTGCTG 
ATGAATAATG CCCTCACCCG TTTGCCTGAC AAACCCCTCT GTCAGCACAT GCCCCGCCTG 
CACTGGCTGG ACTTTGAAGG CAACCGTATC CATAATTTAA GAAATTTGAC TTTTATTTCC 
TGCAATAATT TAACTGTTTT GGTAATGAGG AAGAACAAAA TCAATTACTT AAATGAACAT 
GCATTTACAC ACCTCCAGAA ACTAGATGAA TTGGATTTAG GAAGTAATAA GATTGAAAAT 
CTTCCACCGA ACATATTTAA GGATCTGAAG GAGCTGTCGC AATTGAATAT TTCTTATAAC 
CCAATCCAGA AAATTGAAGT AAATCAATTC GATTGTCTTG CCAAACTCAA GTCTCTCAGC 
CTTGAAGGAA TAGAAATTTC AAATATCCAA CAAAGGATGT TTAGACCGCT CATAAATCTC 
TCTCACATCT ATTTTAAGAA ATTTCAGTAC TGTGGATACG CACCACATGT GCGCAGCTGT 
AAGCCCAACA CGGATGGGAT TTCATCTCTA GAGAACCTCT TGGCAAGCAT CATCCAGAGA 
GTCTTTGTCT GGGTTGTATC TGCCATTACC TGCTTTGGAA ACATTTTTGT TATTTGTATG 
CGACCATACA TCAGATCTGA GAACAAGCTG CATGCCATGT CGATCATTTC TCTCTGCTGT 
GCTGACTGCC TAATGGGGGT GTATCTATTT GTGATTGGTG CCTTTGACCT CAAGTTTCGG 
GGAGAGTACA ATAAGCACGC ACAGCCGTGG ATGGAGAGTG TTCATTGTCA GTTCATGGGG 
TCGTTGGCCA TTCTGTCCAC AGAAGTATCC GTTTTACTTT TAACATTTCT GACATTGGAA 
AAGTACATCT GCATTGTGTA TCCTTTTCGG TGTTTAAGGC CTCGAAAATG CAGAACAATC 
ACGGTTCTGA TTTTCATTTG GATTATTGGG TTTATAGTGG CTTTTGCTCC ACTTGGCAAT 
AAGGAATTTT TCAAAAACTA CTATGGCACC AATGGAGTAT GCTTTCCTCT TCATTCAGAA 
GACACGGGAA GTACTGGAGC CCAGATTTAT TCAGTGGTAA TTTTTCTTGG TATTAACCTG 
GTGGCATTTA TCATCATTGT GTTCTCCTAT GGAAGCATGT TTTACAGTGT TCATCAAAGC 
TCCGTAACAG TAACCGAAAT ACAGAAGCAA GTGAAGAAGG AGGTGGTTCT CGCCAAACGC 
TTTTTCTTTA TCGTTTTCAC CGACGCGCTG TGCTGGATTC CCATCTTCAT ACTGAAATTT 
CTTTCACTGC TTCAGGTGGA AATACCAGAT TCTATTACCT CTTGGGTGGT GATTTTTATT 
CTGCCTATCA ACAGTGCTTT GAACCCAATT ATCTACACGT TGACCACTAG ACCTTTCAAG 
GAAATGATCC ATCAACTCTG GCACAACTAC AGACAAAGAA GGTCTGTTGA CAGGAAAGAG 
ACTCAGAAGG CATATGCTCC ATCATTCATC TGGGTGGAAA TGTGGCCCTT GCAGGAGATG 
TCCTCTGGGT TCATGAAGCC AGGCGCTTTC ACAGACCCCT GTGATCTGTC GCTAGTTTCT 
CAGTCATCTA GACTCAATTC TTATTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501693.1
CDS	64..1953
Translation	MLSLQRNFIRTLPPNGFRKYHELQKLCLQNNRIHSVSVSAFRGLRSLTKLYLSHNRITFLKPGVFEDLHRLEWLIIEDNHLSRISPLTFYGLNSLILLVLMNNALTRLPDKPLCQHMPRLHWLDFEGNRIHNLRNLTFISCNNLTVLVMRKNKINYLNEHAFTHLQKLDELDLGSNKIENLPPNIFKDLKELSQLNISYNPIQKIEVNQFDCLAKLKSLSLEGIEISNIQQRMFRPLINLSHIYFKKFQYCGYAPHVRSCKPNTDGISSLENLLASIIQRVFVWVVSAITCFGNIFVICMRPYIRSENKLHAMSIISLCCADCLMGVYLFVIGAFDLKFRGEYNKHAQPWMESVHCQFMGSLAILSTEVSVLLLTFLTLEKYICIVYPFRCLRPRKCRTITVLIFIWIIGFIVAFAPLGNKEFFKNYYGTNGVCFPLHSEDTGSTGAQIYSVVIFLGINLVAFIIIVFSYGSMFYSVHQSSVTVTEIQKQVKKEVVLAKRFFFIVFTDALCWIPIFILKFLSLLQVEIPDSITSWVVIFILPINSALNPIIYTLTTRPFKEMIHQLWHNYRQRRSVDRKETQKAYAPSFIWVEMWPLQEMSSGFMKPGAFTDPCDLSLVSQSSRLNSYS

Target ORF information:

RefSeq Version	XM_006501630.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501630.2

ORF Insert Sequence:

ATGCTGTCCC TGCAGAGGAA CTTCATAAGA ACACTCCCTC CCAACGGCTT CAGGAAGTAC 
CATGAGCTGC AGAAGCTGTG CCTGCAAAAC AATAGGATTC ACTCCGTGAG CGTCTCTGCC 
TTCAGAGGAC TGCGCAGCCT CACTAAACTG TACCTCAGTC ATAACAGAAT AACCTTCCTA 
AAGCCAGGAG TTTTTGAAGA TCTCCACAGA CTGGAATGGC TGATAATTGA AGACAATCAC 
CTCAGTCGAA TTTCCCCACT CACATTTTAC GGACTAAATT CTCTTATTCT CTTAGTGCTG 
ATGAATAATG CCCTCACCCG TTTGCCTGAC AAACCCCTCT GTCAGCACAT GCCCCGCCTG 
CACTGGCTGG ACTTTGAAGG CAACCGTATC CATAATTTAA GAAATTTGAC TTTTATTTCC 
TGCAATAATT TAACTGTTTT GGTAATGAGG AAGAACAAAA TCAATTACTT AAATGAACAT 
GCATTTACAC ACCTCCAGAA ACTAGATGAA TTGGATTTAG GAAGTAATAA GATTGAAAAT 
CTTCCACCGA ACATATTTAA GGATCTGAAG GAGCTGTCGC AATTGAATAT TTCTTATAAC 
CCAATCCAGA AAATTGAAGT AAATCAATTC GATTGTCTTG CCAAACTCAA GTCTCTCAGC 
CTTGAAGGAA TAGAAATTTC AAATATCCAA CAAAGGATGT TTAGACCGCT CATAAATCTC 
TCTCACATCT ATTTTAAGAA ATTTCAGTAC TGTGGATACG CACCACATGT GCGCAGCTGT 
AAGCCCAACA CGGATGGGAT TTCATCTCTA GAGAACCTCT TGGCAAGCAT CATCCAGAGA 
GTCTTTGTCT GGGTTGTATC TGCCATTACC TGCTTTGGAA ACATTTTTGT TATTTGTATG 
CGACCATACA TCAGATCTGA GAACAAGCTG CATGCCATGT CGATCATTTC TCTCTGCTGT 
GCTGACTGCC TAATGGGGGT GTATCTATTT GTGATTGGTG CCTTTGACCT CAAGTTTCGG 
GGAGAGTACA ATAAGCACGC ACAGCCGTGG ATGGAGAGTG TTCATTGTCA GTTCATGGGG 
TCGTTGGCCA TTCTGTCCAC AGAAGTATCC GTTTTACTTT TAACATTTCT GACATTGGAA 
AAGTACATCT GCATTGTGTA TCCTTTTCGG TGTTTAAGGC CTCGAAAATG CAGAACAATC 
ACGGTTCTGA TTTTCATTTG GATTATTGGG TTTATAGTGG CTTTTGCTCC ACTTGGCAAT 
AAGGAATTTT TCAAAAACTA CTATGGCACC AATGGAGTAT GCTTTCCTCT TCATTCAGAA 
GACACGGGAA GTACTGGAGC CCAGATTTAT TCAGTGGTAA TTTTTCTTGG TATTAACCTG 
GTGGCATTTA TCATCATTGT GTTCTCCTAT GGAAGCATGT TTTACAGTGT TCATCAAAGC 
TCCGTAACAG TAACCGAAAT ACAGAAGCAA GTGAAGAAGG AGGTGGTTCT CGCCAAACGC 
TTTTTCTTTA TCGTTTTCAC CGACGCGCTG TGCTGGATTC CCATCTTCAT ACTGAAATTT 
CTTTCACTGC TTCAGGTGGA AATACCAGAT TCTATTACCT CTTGGGTGGT GATTTTTATT 
CTGCCTATCA ACAGTGCTTT GAACCCAATT ATCTACACGT TGACCACTAG ACCTTTCAAG 
GAAATGATCC ATCAACTCTG GCACAACTAC AGACAAAGAA GGTCTGTTGA CAGGAAAGAG 
ACTCAGAAGG CATATGCTCC ATCATTCATC TGGGTGGAAA TGTGGCCCTT GCAGGAGATG 
TCCTCTGGGT TCATGAAGCC AGGCGCTTTC ACAGACCCCT GTGATCTGTC GCTAGTTTCT 
CAGTCATCTA GACTCAATTC TTATTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rxfp1 ( XM_006501629.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501692.1 Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu32019
Clone ID Related Accession (Same CDS sequence)	XM_006501629.2
Accession Version	XM_006501629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	relaxin receptor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006501629.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCTG GTCCATTCTT CTTCTGCATC TTCATTATTG GAAAATATTT CACTCTCGGA 
AGTGCACAGG ATGTCAGTTG TCCCCTCGGC TCCTTCCCCT GTGGGAACAT GAGCAGGTGC 
CTGCCTCAGC TGCTTCACTG CAATGGTGTG GACGACTGCG GGAACCGAGC TGATGAGGAC 
CACTGCGGAG ACAACAATGG GTGGTCTCTG CAACTGGACA AATATTTTGC TAATTACTAT 
AAACTGGCTT CCACTAACTC CTTTGAGGCA GAAACTTCCG AATGCTTGGT TGGCTCTGTG 
CCCATGCATT GTTTGTGCCG AGATCTAGAA CTTGACTGTG ATGAAGCCAA TTTACGAGCT 
GTCCCATCAG TTTCTTCCAA TGTGACTGTA ATGTCCCTGC AGAGGAACTT CATAAGAACA 
CTCCCTCCCA ACGGCTTCAG GAAGTACCAT GAGCTGCAGA AGCTGTGCCT GCAAAACAAT 
AGGATTCACT CCGTGAGCGT CTCTGCCTTC AGAGGACTGC GCAGCCTCAC TAAACTGTAC 
CTCAGTCATA ACAGAATAAC CTTCCTAAAG CCAGGAGTTT TTGAAGATCT CCACAGACTG 
GAATGGCTGA TAATTGAAGA CAATCACCTC AGTCGAATTT CCCCACTCAC ATTTTACGGA 
CTAAATTCTC TTATTCTCTT AGTGCTGATG AATAATGCCC TCACCCGTTT GCCTGACAAA 
CCCCTCTGTC AGCACATGCC CCGCCTGCAC TGGCTGGACT TTGAAGGCAA CCGTATCCAT 
AATTTAAGAA ATTTGACTTT TATTTCCTGC AATAATTTAA CTGTTTTGGT AATGAGGAAG 
AACAAAATCA ATTACTTAAA TGAACATGCA TTTACACACC TCCAGAAACT AGATGAATTG 
GATTTAGGAA GTAATAAGAT TGAAAATCTT CCACCGAACA TATTTAAGGA TCTGAAGGAG 
CTGTCGCAAT TGAATATTTC TTATAACCCA ATCCAGAAAA TTGAAGTAAA TCAATTCGAT 
TGTCTTGCCA AACTCAAGTC TCTCCTTGAA GGAATAGAAA TTTCAAATAT CCAACAAAGG 
ATGTTTAGAC CGCTCATAAA TCTCTCTCAC ATCTATTTTA AGAAATTTCA GTACTGTGGA 
TACGCACCAC ATGTGCGCAG CTGTAAGCCC AACACGGATG GGATTTCATC TCTAGAGAAC 
CTCTTGGCAA GCATCATCCA GAGAGTCTTT GTCTGGGTTG TATCTGCCAT TACCTGCTTT 
GGAAACATTT TTGTTATTTG TATGCGACCA TACATCAGAT CTGAGAACAA GCTGCATGCC 
ATGTCGATCA TTTCTCTCTG CTGTGCTGAC TGCCTAATGG GGGTGTATCT ATTTGTGATT 
GGTGCCTTTG ACCTCAAGTT TCGGGGAGAG TACAATAAGC ACGCACAGCC GTGGATGGAG 
AGTGTTCATT GTCAGTTCAT GGGGTCGTTG GCCATTCTGT CCACAGAAGT ATCCGTTTTA 
CTTTTAACAT TTCTGACATT GGAAAAGTAC ATCTGCATTG TGTATCCTTT TCGGTGTTTA 
AGGCCTCGAA AATGCAGAAC AATCACGGTT CTGATTTTCA TTTGGATTAT TGGGTTTATA 
GTGGCTTTTG CTCCACTTGG CAATAAGGAA TTTTTCAAAA ACTACTATGG CACCAATGGA 
GTATGCTTTC CTCTTCATTC AGAAGACACG GGAAGTACTG GAGCCCAGAT TTATTCAGTG 
GTAATTTTTC TTGGTATTAA CCTGGTGGCA TTTATCATCA TTGTGTTCTC CTATGGAAGC 
ATGTTTTACA GTGTTCATCA AAGCTCCGTA ACAGTAACCG AAATACAGAA GCAAGTGAAG 
AAGGAGGTGG TTCTCGCCAA ACGCTTTTTC TTTATCGTTT TCACCGACGC GCTGTGCTGG 
ATTCCCATCT TCATACTGAA ATTTCTTTCA CTGCTTCAGG TGGAAATACC AGATTCTATT 
ACCTCTTGGG TGGTGATTTT TATTCTGCCT ATCAACAGTG CTTTGAACCC AATTATCTAC 
ACGTTGACCA CTAGACCTTT CAAGGAAATG ATCCATCAAC TCTGGCACAA CTACAGACAA 
AGAAGGTCTG TTGACAGGAA AGAGACTCAG AAGGCATATG CTCCATCATT CATCTGGGTG 
GAAATGTGGC CCTTGCAGGA GATGTCCTCT GGGTTCATGA AGCCAGGCGC TTTCACAGAC 
CCCTGTGATC TGTCGCTAGT TTCTCAGTCA TCTAGACTCA ATTCTTATTC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501692.1
CDS	158..2431
Translation	MTSGPFFFCIFIIGKYFTLGSAQDVSCPLGSFPCGNMSRCLPQLLHCNGVDDCGNRADEDHCGDNNGWSLQLDKYFANYYKLASTNSFEAETSECLVGSVPMHCLCRDLELDCDEANLRAVPSVSSNVTVMSLQRNFIRTLPPNGFRKYHELQKLCLQNNRIHSVSVSAFRGLRSLTKLYLSHNRITFLKPGVFEDLHRLEWLIIEDNHLSRISPLTFYGLNSLILLVLMNNALTRLPDKPLCQHMPRLHWLDFEGNRIHNLRNLTFISCNNLTVLVMRKNKINYLNEHAFTHLQKLDELDLGSNKIENLPPNIFKDLKELSQLNISYNPIQKIEVNQFDCLAKLKSLLEGIEISNIQQRMFRPLINLSHIYFKKFQYCGYAPHVRSCKPNTDGISSLENLLASIIQRVFVWVVSAITCFGNIFVICMRPYIRSENKLHAMSIISLCCADCLMGVYLFVIGAFDLKFRGEYNKHAQPWMESVHCQFMGSLAILSTEVSVLLLTFLTLEKYICIVYPFRCLRPRKCRTITVLIFIWIIGFIVAFAPLGNKEFFKNYYGTNGVCFPLHSEDTGSTGAQIYSVVIFLGINLVAFIIIVFSYGSMFYSVHQSSVTVTEIQKQVKKEVVLAKRFFFIVFTDALCWIPIFILKFLSLLQVEIPDSITSWVVIFILPINSALNPIIYTLTTRPFKEMIHQLWHNYRQRRSVDRKETQKAYAPSFIWVEMWPLQEMSSGFMKPGAFTDPCDLSLVSQSSRLNSYS

Target ORF information:

RefSeq Version	XM_006501629.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501629.2

ORF Insert Sequence:

ATGACATCTG GTCCATTCTT CTTCTGCATC TTCATTATTG GAAAATATTT CACTCTCGGA 
AGTGCACAGG ATGTCAGTTG TCCCCTCGGC TCCTTCCCCT GTGGGAACAT GAGCAGGTGC 
CTGCCTCAGC TGCTTCACTG CAATGGTGTG GACGACTGCG GGAACCGAGC TGATGAGGAC 
CACTGCGGAG ACAACAATGG GTGGTCTCTG CAACTGGACA AATATTTTGC TAATTACTAT 
AAACTGGCTT CCACTAACTC CTTTGAGGCA GAAACTTCCG AATGCTTGGT TGGCTCTGTG 
CCCATGCATT GTTTGTGCCG AGATCTAGAA CTTGACTGTG ATGAAGCCAA TTTACGAGCT 
GTCCCATCAG TTTCTTCCAA TGTGACTGTA ATGTCCCTGC AGAGGAACTT CATAAGAACA 
CTCCCTCCCA ACGGCTTCAG GAAGTACCAT GAGCTGCAGA AGCTGTGCCT GCAAAACAAT 
AGGATTCACT CCGTGAGCGT CTCTGCCTTC AGAGGACTGC GCAGCCTCAC TAAACTGTAC 
CTCAGTCATA ACAGAATAAC CTTCCTAAAG CCAGGAGTTT TTGAAGATCT CCACAGACTG 
GAATGGCTGA TAATTGAAGA CAATCACCTC AGTCGAATTT CCCCACTCAC ATTTTACGGA 
CTAAATTCTC TTATTCTCTT AGTGCTGATG AATAATGCCC TCACCCGTTT GCCTGACAAA 
CCCCTCTGTC AGCACATGCC CCGCCTGCAC TGGCTGGACT TTGAAGGCAA CCGTATCCAT 
AATTTAAGAA ATTTGACTTT TATTTCCTGC AATAATTTAA CTGTTTTGGT AATGAGGAAG 
AACAAAATCA ATTACTTAAA TGAACATGCA TTTACACACC TCCAGAAACT AGATGAATTG 
GATTTAGGAA GTAATAAGAT TGAAAATCTT CCACCGAACA TATTTAAGGA TCTGAAGGAG 
CTGTCGCAAT TGAATATTTC TTATAACCCA ATCCAGAAAA TTGAAGTAAA TCAATTCGAT 
TGTCTTGCCA AACTCAAGTC TCTCCTTGAA GGAATAGAAA TTTCAAATAT CCAACAAAGG 
ATGTTTAGAC CGCTCATAAA TCTCTCTCAC ATCTATTTTA AGAAATTTCA GTACTGTGGA 
TACGCACCAC ATGTGCGCAG CTGTAAGCCC AACACGGATG GGATTTCATC TCTAGAGAAC 
CTCTTGGCAA GCATCATCCA GAGAGTCTTT GTCTGGGTTG TATCTGCCAT TACCTGCTTT 
GGAAACATTT TTGTTATTTG TATGCGACCA TACATCAGAT CTGAGAACAA GCTGCATGCC 
ATGTCGATCA TTTCTCTCTG CTGTGCTGAC TGCCTAATGG GGGTGTATCT ATTTGTGATT 
GGTGCCTTTG ACCTCAAGTT TCGGGGAGAG TACAATAAGC ACGCACAGCC GTGGATGGAG 
AGTGTTCATT GTCAGTTCAT GGGGTCGTTG GCCATTCTGT CCACAGAAGT ATCCGTTTTA 
CTTTTAACAT TTCTGACATT GGAAAAGTAC ATCTGCATTG TGTATCCTTT TCGGTGTTTA 
AGGCCTCGAA AATGCAGAAC AATCACGGTT CTGATTTTCA TTTGGATTAT TGGGTTTATA 
GTGGCTTTTG CTCCACTTGG CAATAAGGAA TTTTTCAAAA ACTACTATGG CACCAATGGA 
GTATGCTTTC CTCTTCATTC AGAAGACACG GGAAGTACTG GAGCCCAGAT TTATTCAGTG 
GTAATTTTTC TTGGTATTAA CCTGGTGGCA TTTATCATCA TTGTGTTCTC CTATGGAAGC 
ATGTTTTACA GTGTTCATCA AAGCTCCGTA ACAGTAACCG AAATACAGAA GCAAGTGAAG 
AAGGAGGTGG TTCTCGCCAA ACGCTTTTTC TTTATCGTTT TCACCGACGC GCTGTGCTGG 
ATTCCCATCT TCATACTGAA ATTTCTTTCA CTGCTTCAGG TGGAAATACC AGATTCTATT 
ACCTCTTGGG TGGTGATTTT TATTCTGCCT ATCAACAGTG CTTTGAACCC AATTATCTAC 
ACGTTGACCA CTAGACCTTT CAAGGAAATG ATCCATCAAC TCTGGCACAA CTACAGACAA 
AGAAGGTCTG TTGACAGGAA AGAGACTCAG AAGGCATATG CTCCATCATT CATCTGGGTG 
GAAATGTGGC CCTTGCAGGA GATGTCCTCT GGGTTCATGA AGCCAGGCGC TTTCACAGAC 
CCCTGTGATC TGTCGCTAGT TTCTCAGTCA TCTAGACTCA ATTCTTATTC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rxfp1 ( NM_212452.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_997617.1 Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), mRNA.

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CloneID	OMu10195
Clone ID Related Accession (Same CDS sequence)	NM_212452.2 , NM_212452.1
Accession Version	NM_212452.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Mus musculus(house mouse)
Product	relaxin receptor 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC159281.3, AY509975.1, AK137655.1 and AC122462.5. On Nov 10, 2017 this sequence version replaced NM_212452.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.51830.1, AY509975.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGACATCTG GTCCATTCTT CTTCTGCATC TTCATTATTG GAAAATATTT CACTCTCGGA 
AGTGCACAGG ATGTCAGTTG TCCCCTCGGC TCCTTCCCCT GTGGGAACAT GAGCAGGTGC 
CTGCCTCAGC TGCTTCACTG CAATGGTGTG GACGACTGCG GGAACCGAGC TGATGAGGAC 
CACTGCGGAG ACAACAATGG GTGGTCTCTG CAACTGGACA AATATTTTGC TAATTACTAT 
AAACTGGCTT CCACTAACTC CTTTGAGGCA GAAACTTCCG AATGCTTGGT TGGCTCTGTG 
CCCATGCATT GTTTGTGCCG AGATCTAGAA CTTGACTGTG ATGAAGCCAA TTTACGAGCT 
GTCCCATCAG TTTCTTCCAA TGTGACTGTA ATGTCCCTGC AGAGGAACTT CATAAGAACA 
CTCCCTCCCA ACGGCTTCAG GAAGTACCAT GAGCTGCAGA AGCTGTGCCT GCAAAACAAT 
AGGATTCACT CCGTGAGCGT CTCTGCCTTC AGAGGACTGC GCAGCCTCAC TAAACTGTAC 
CTCAGTCATA ACAGAATAAC CTTCCTAAAG CCAGGAGTTT TTGAAGATCT CCACAGACTG 
GAATGGCTGA TAATTGAAGA CAATCACCTC AGTCGAATTT CCCCACTCAC ATTTTACGGA 
CTAAATTCTC TTATTCTCTT AGTGCTGATG AATAATGCCC TCACCCGTTT GCCTGACAAA 
CCCCTCTGTC AGCACATGCC CCGCCTGCAC TGGCTGGACT TTGAAGGCAA CCGTATCCAT 
AATTTAAGAA ATTTGACTTT TATTTCCTGC AATAATTTAA CTGTTTTGGT AATGAGGAAG 
AACAAAATCA ATTACTTAAA TGAACATGCA TTTACACACC TCCAGAAACT AGATGAATTG 
GATTTAGGAA GTAATAAGAT TGAAAATCTT CCACCGAACA TATTTAAGGA TCTGAAGGAG 
CTGTCGCAAT TGAATATTTC TTATAACCCA ATCCAGAAAA TTGAAGTAAA TCAATTCGAT 
TGTCTTGCCA AACTCAAGTC TCTCAGCCTT GAAGGAATAG AAATTTCAAA TATCCAACAA 
AGGATGTTTA GACCGCTCAT AAATCTCTCT CACATCTATT TTAAGAAATT TCAGTACTGT 
GGATACGCAC CACATGTGCG CAGCTGTAAG CCCAACACGG ATGGGATTTC ATCTCTAGAG 
AACCTCTTGG CAAGCATCAT CCAGAGAGTC TTTGTCTGGG TTGTATCTGC CATTACCTGC 
TTTGGAAACA TTTTTGTTAT TTGTATGCGA CCATACATCA GATCTGAGAA CAAGCTGCAT 
GCCATGTCGA TCATTTCTCT CTGCTGTGCT GACTGCCTAA TGGGGGTGTA TCTATTTGTG 
ATTGGTGCCT TTGACCTCAA GTTTCGGGGA GAGTACAATA AGCACGCACA GCCGTGGATG 
GAGAGTGTTC ATTGTCAGTT CATGGGGTCG TTGGCCATTC TGTCCACAGA AGTATCCGTT 
TTACTTTTAA CATTTCTGAC ATTGGAAAAG TACATCTGCA TTGTGTATCC TTTTCGGTGT 
TTAAGGCCTC GAAAATGCAG AACAATCACG GTTCTGATTT TCATTTGGAT TATTGGGTTT 
ATAGTGGCTT TTGCTCCACT TGGCAATAAG GAATTTTTCA AAAACTACTA TGGCACCAAT 
GGAGTATGCT TTCCTCTTCA TTCAGAAGAC ACGGGAAGTA CTGGAGCCCA GATTTATTCA 
GTGGTAATTT TTCTTGGTAT TAACCTGGTG GCATTTATCA TCATTGTGTT CTCCTATGGA 
AGCATGTTTT ACAGTGTTCA TCAAAGCTCC GTAACAGTAA CCGAAATACA GAAGCAAGTG 
AAGAAGGAGG TGGTTCTCGC CAAACGCTTT TTCTTTATCG TTTTCACCGA CGCGCTGTGC 
TGGATTCCCA TCTTCATACT GAAATTTCTT TCACTGCTTC AGGTGGAAAT ACCAGATTCT 
ATTACCTCTT GGGTGGTGAT TTTTATTCTG CCTATCAACA GTGCTTTGAA CCCAATTATC 
TACACGTTGA CCACTAGACC TTTCAAGGAA ATGATCCATC AACTCTGGCA CAACTACAGA 
CAAAGAAGGT CTGTTGACAG GAAAGAGACT CAGAAGGCAT ATGCTCCATC ATTCATCTGG 
GTGGAAATGT GGCCCTTGCA GGAGATGTCC TCTGGGTTCA TGAAGCCAGG CGCTTTCACA 
GACCCCTGTG ATCTGTCGCT AGTTTCTCAG TCATCTAGAC TCAATTCTTA TTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997617.1
CDS	158..2434
Translation	MTSGPFFFCIFIIGKYFTLGSAQDVSCPLGSFPCGNMSRCLPQLLHCNGVDDCGNRADEDHCGDNNGWSLQLDKYFANYYKLASTNSFEAETSECLVGSVPMHCLCRDLELDCDEANLRAVPSVSSNVTVMSLQRNFIRTLPPNGFRKYHELQKLCLQNNRIHSVSVSAFRGLRSLTKLYLSHNRITFLKPGVFEDLHRLEWLIIEDNHLSRISPLTFYGLNSLILLVLMNNALTRLPDKPLCQHMPRLHWLDFEGNRIHNLRNLTFISCNNLTVLVMRKNKINYLNEHAFTHLQKLDELDLGSNKIENLPPNIFKDLKELSQLNISYNPIQKIEVNQFDCLAKLKSLSLEGIEISNIQQRMFRPLINLSHIYFKKFQYCGYAPHVRSCKPNTDGISSLENLLASIIQRVFVWVVSAITCFGNIFVICMRPYIRSENKLHAMSIISLCCADCLMGVYLFVIGAFDLKFRGEYNKHAQPWMESVHCQFMGSLAILSTEVSVLLLTFLTLEKYICIVYPFRCLRPRKCRTITVLIFIWIIGFIVAFAPLGNKEFFKNYYGTNGVCFPLHSEDTGSTGAQIYSVVIFLGINLVAFIIIVFSYGSMFYSVHQSSVTVTEIQKQVKKEVVLAKRFFFIVFTDALCWIPIFILKFLSLLQVEIPDSITSWVVIFILPINSALNPIIYTLTTRPFKEMIHQLWHNYRQRRSVDRKETQKAYAPSFIWVEMWPLQEMSSGFMKPGAFTDPCDLSLVSQSSRLNSYS

Target ORF information:

RefSeq Version	NM_212452.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212452.2

ORF Insert Sequence:

ATGACATCTG GTCCATTCTT CTTCTGCATC TTCATTATTG GAAAATATTT CACTCTCGGA 
AGTGCACAGG ATGTCAGTTG TCCCCTCGGC TCCTTCCCCT GTGGGAACAT GAGCAGGTGC 
CTGCCTCAGC TGCTTCACTG CAATGGTGTG GACGACTGCG GGAACCGAGC TGATGAGGAC 
CACTGCGGAG ACAACAATGG GTGGTCTCTG CAACTGGACA AATATTTTGC TAATTACTAT 
AAACTGGCTT CCACTAACTC CTTTGAGGCA GAAACTTCCG AATGCTTGGT TGGCTCTGTG 
CCCATGCATT GTTTGTGCCG AGATCTAGAA CTTGACTGTG ATGAAGCCAA TTTACGAGCT 
GTCCCATCAG TTTCTTCCAA TGTGACTGTA ATGTCCCTGC AGAGGAACTT CATAAGAACA 
CTCCCTCCCA ACGGCTTCAG GAAGTACCAT GAGCTGCAGA AGCTGTGCCT GCAAAACAAT 
AGGATTCACT CCGTGAGCGT CTCTGCCTTC AGAGGACTGC GCAGCCTCAC TAAACTGTAC 
CTCAGTCATA ACAGAATAAC CTTCCTAAAG CCAGGAGTTT TTGAAGATCT CCACAGACTG 
GAATGGCTGA TAATTGAAGA CAATCACCTC AGTCGAATTT CCCCACTCAC ATTTTACGGA 
CTAAATTCTC TTATTCTCTT AGTGCTGATG AATAATGCCC TCACCCGTTT GCCTGACAAA 
CCCCTCTGTC AGCACATGCC CCGCCTGCAC TGGCTGGACT TTGAAGGCAA CCGTATCCAT 
AATTTAAGAA ATTTGACTTT TATTTCCTGC AATAATTTAA CTGTTTTGGT AATGAGGAAG 
AACAAAATCA ATTACTTAAA TGAACATGCA TTTACACACC TCCAGAAACT AGATGAATTG 
GATTTAGGAA GTAATAAGAT TGAAAATCTT CCACCGAACA TATTTAAGGA TCTGAAGGAG 
CTGTCGCAAT TGAATATTTC TTATAACCCA ATCCAGAAAA TTGAAGTAAA TCAATTCGAT 
TGTCTTGCCA AACTCAAGTC TCTCAGCCTT GAAGGAATAG AAATTTCAAA TATCCAACAA 
AGGATGTTTA GACCGCTCAT AAATCTCTCT CACATCTATT TTAAGAAATT TCAGTACTGT 
GGATACGCAC CACATGTGCG CAGCTGTAAG CCCAACACGG ATGGGATTTC ATCTCTAGAG 
AACCTCTTGG CAAGCATCAT CCAGAGAGTC TTTGTCTGGG TTGTATCTGC CATTACCTGC 
TTTGGAAACA TTTTTGTTAT TTGTATGCGA CCATACATCA GATCTGAGAA CAAGCTGCAT 
GCCATGTCGA TCATTTCTCT CTGCTGTGCT GACTGCCTAA TGGGGGTGTA TCTATTTGTG 
ATTGGTGCCT TTGACCTCAA GTTTCGGGGA GAGTACAATA AGCACGCACA GCCGTGGATG 
GAGAGTGTTC ATTGTCAGTT CATGGGGTCG TTGGCCATTC TGTCCACAGA AGTATCCGTT 
TTACTTTTAA CATTTCTGAC ATTGGAAAAG TACATCTGCA TTGTGTATCC TTTTCGGTGT 
TTAAGGCCTC GAAAATGCAG AACAATCACG GTTCTGATTT TCATTTGGAT TATTGGGTTT 
ATAGTGGCTT TTGCTCCACT TGGCAATAAG GAATTTTTCA AAAACTACTA TGGCACCAAT 
GGAGTATGCT TTCCTCTTCA TTCAGAAGAC ACGGGAAGTA CTGGAGCCCA GATTTATTCA 
GTGGTAATTT TTCTTGGTAT TAACCTGGTG GCATTTATCA TCATTGTGTT CTCCTATGGA 
AGCATGTTTT ACAGTGTTCA TCAAAGCTCC GTAACAGTAA CCGAAATACA GAAGCAAGTG 
AAGAAGGAGG TGGTTCTCGC CAAACGCTTT TTCTTTATCG TTTTCACCGA CGCGCTGTGC 
TGGATTCCCA TCTTCATACT GAAATTTCTT TCACTGCTTC AGGTGGAAAT ACCAGATTCT 
ATTACCTCTT GGGTGGTGAT TTTTATTCTG CCTATCAACA GTGCTTTGAA CCCAATTATC 
TACACGTTGA CCACTAGACC TTTCAAGGAA ATGATCCATC AACTCTGGCA CAACTACAGA 
CAAAGAAGGT CTGTTGACAG GAAAGAGACT CAGAAGGCAT ATGCTCCATC ATTCATCTGG 
GTGGAAATGT GGCCCTTGCA GGAGATGTCC TCTGGGTTCA TGAAGCCAGG CGCTTTCACA 
GACCCCTGTG ATCTGTCGCT AGTTTCTCAG TCATCTAGAC TCAATTCTTA TTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rxfp1 ( NM_212452.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_997617.1 Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), mRNA.

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CloneID	OMu10195
Clone ID Related Accession (Same CDS sequence)	NM_212452.2 , NM_212452.1
Accession Version	NM_212452.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-25
Organism	Mus musculus(House mouse)
Product	relaxin receptor 1 precursor
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY509975.1. On Aug 30, 2004 this sequence version replaced XM_355441.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY509975.1, SRR1660811.51830.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGACATCTG GTCCATTCTT CTTCTGCATC TTCATTATTG GAAAATATTT CACTCTCGGA 
AGTGCACAGG ATGTCAGTTG TCCCCTCGGC TCCTTCCCCT GTGGGAACAT GAGCAGGTGC 
CTGCCTCAGC TGCTTCACTG CAATGGTGTG GACGACTGCG GGAACCGAGC TGATGAGGAC 
CACTGCGGAG ACAACAATGG GTGGTCTCTG CAACTGGACA AATATTTTGC TAATTACTAT 
AAACTGGCTT CCACTAACTC CTTTGAGGCA GAAACTTCCG AATGCTTGGT TGGCTCTGTG 
CCCATGCATT GTTTGTGCCG AGATCTAGAA CTTGACTGTG ATGAAGCCAA TTTACGAGCT 
GTCCCATCAG TTTCTTCCAA TGTGACTGTA ATGTCCCTGC AGAGGAACTT CATAAGAACA 
CTCCCTCCCA ACGGCTTCAG GAAGTACCAT GAGCTGCAGA AGCTGTGCCT GCAAAACAAT 
AGGATTCACT CCGTGAGCGT CTCTGCCTTC AGAGGACTGC GCAGCCTCAC TAAACTGTAC 
CTCAGTCATA ACAGAATAAC CTTCCTAAAG CCAGGAGTTT TTGAAGATCT CCACAGACTG 
GAATGGCTGA TAATTGAAGA CAATCACCTC AGTCGAATTT CCCCACTCAC ATTTTACGGA 
CTAAATTCTC TTATTCTCTT AGTGCTGATG AATAATGCCC TCACCCGTTT GCCTGACAAA 
CCCCTCTGTC AGCACATGCC CCGCCTGCAC TGGCTGGACT TTGAAGGCAA CCGTATCCAT 
AATTTAAGAA ATTTGACTTT TATTTCCTGC AATAATTTAA CTGTTTTGGT AATGAGGAAG 
AACAAAATCA ATTACTTAAA TGAACATGCA TTTACACACC TCCAGAAACT AGATGAATTG 
GATTTAGGAA GTAATAAGAT TGAAAATCTT CCACCGAACA TATTTAAGGA TCTGAAGGAG 
CTGTCGCAAT TGAATATTTC TTATAACCCA ATCCAGAAAA TTGAAGTAAA TCAATTCGAT 
TGTCTTGCCA AACTCAAGTC TCTCAGCCTT GAAGGAATAG AAATTTCAAA TATCCAACAA 
AGGATGTTTA GACCGCTCAT AAATCTCTCT CACATCTATT TTAAGAAATT TCAGTACTGT 
GGATACGCAC CACATGTGCG CAGCTGTAAG CCCAACACGG ATGGGATTTC ATCTCTAGAG 
AACCTCTTGG CAAGCATCAT CCAGAGAGTC TTTGTCTGGG TTGTATCTGC CATTACCTGC 
TTTGGAAACA TTTTTGTTAT TTGTATGCGA CCATACATCA GATCTGAGAA CAAGCTGCAT 
GCCATGTCGA TCATTTCTCT CTGCTGTGCT GACTGCCTAA TGGGGGTGTA TCTATTTGTG 
ATTGGTGCCT TTGACCTCAA GTTTCGGGGA GAGTACAATA AGCACGCACA GCCGTGGATG 
GAGAGTGTTC ATTGTCAGTT CATGGGGTCG TTGGCCATTC TGTCCACAGA AGTATCCGTT 
TTACTTTTAA CATTTCTGAC ATTGGAAAAG TACATCTGCA TTGTGTATCC TTTTCGGTGT 
TTAAGGCCTC GAAAATGCAG AACAATCACG GTTCTGATTT TCATTTGGAT TATTGGGTTT 
ATAGTGGCTT TTGCTCCACT TGGCAATAAG GAATTTTTCA AAAACTACTA TGGCACCAAT 
GGAGTATGCT TTCCTCTTCA TTCAGAAGAC ACGGGAAGTA CTGGAGCCCA GATTTATTCA 
GTGGTAATTT TTCTTGGTAT TAACCTGGTG GCATTTATCA TCATTGTGTT CTCCTATGGA 
AGCATGTTTT ACAGTGTTCA TCAAAGCTCC GTAACAGTAA CCGAAATACA GAAGCAAGTG 
AAGAAGGAGG TGGTTCTCGC CAAACGCTTT TTCTTTATCG TTTTCACCGA CGCGCTGTGC 
TGGATTCCCA TCTTCATACT GAAATTTCTT TCACTGCTTC AGGTGGAAAT ACCAGATTCT 
ATTACCTCTT GGGTGGTGAT TTTTATTCTG CCTATCAACA GTGCTTTGAA CCCAATTATC 
TACACGTTGA CCACTAGACC TTTCAAGGAA ATGATCCATC AACTCTGGCA CAACTACAGA 
CAAAGAAGGT CTGTTGACAG GAAAGAGACT CAGAAGGCAT ATGCTCCATC ATTCATCTGG 
GTGGAAATGT GGCCCTTGCA GGAGATGTCC TCTGGGTTCA TGAAGCCAGG CGCTTTCACA 
GACCCCTGTG ATCTGTCGCT AGTTTCTCAG TCATCTAGAC TCAATTCTTA TTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997617.1
CDS	1..2277
Translation	MTSGPFFFCIFIIGKYFTLGSAQDVSCPLGSFPCGNMSRCLPQLLHCNGVDDCGNRADEDHCGDNNGWSLQLDKYFANYYKLASTNSFEAETSECLVGSVPMHCLCRDLELDCDEANLRAVPSVSSNVTVMSLQRNFIRTLPPNGFRKYHELQKLCLQNNRIHSVSVSAFRGLRSLTKLYLSHNRITFLKPGVFEDLHRLEWLIIEDNHLSRISPLTFYGLNSLILLVLMNNALTRLPDKPLCQHMPRLHWLDFEGNRIHNLRNLTFISCNNLTVLVMRKNKINYLNEHAFTHLQKLDELDLGSNKIENLPPNIFKDLKELSQLNISYNPIQKIEVNQFDCLAKLKSLSLEGIEISNIQQRMFRPLINLSHIYFKKFQYCGYAPHVRSCKPNTDGISSLENLLASIIQRVFVWVVSAITCFGNIFVICMRPYIRSENKLHAMSIISLCCADCLMGVYLFVIGAFDLKFRGEYNKHAQPWMESVHCQFMGSLAILSTEVSVLLLTFLTLEKYICIVYPFRCLRPRKCRTITVLIFIWIIGFIVAFAPLGNKEFFKNYYGTNGVCFPLHSEDTGSTGAQIYSVVIFLGINLVAFIIIVFSYGSMFYSVHQSSVTVTEIQKQVKKEVVLAKRFFFIVFTDALCWIPIFILKFLSLLQVEIPDSITSWVVIFILPINSALNPIIYTLTTRPFKEMIHQLWHNYRQRRSVDRKETQKAYAPSFIWVEMWPLQEMSSGFMKPGAFTDPCDLSLVSQSSRLNSYS

Target ORF information:

RefSeq Version	NM_212452.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212452.1

ORF Insert Sequence:

ATGACATCTG GTCCATTCTT CTTCTGCATC TTCATTATTG GAAAATATTT CACTCTCGGA 
AGTGCACAGG ATGTCAGTTG TCCCCTCGGC TCCTTCCCCT GTGGGAACAT GAGCAGGTGC 
CTGCCTCAGC TGCTTCACTG CAATGGTGTG GACGACTGCG GGAACCGAGC TGATGAGGAC 
CACTGCGGAG ACAACAATGG GTGGTCTCTG CAACTGGACA AATATTTTGC TAATTACTAT 
AAACTGGCTT CCACTAACTC CTTTGAGGCA GAAACTTCCG AATGCTTGGT TGGCTCTGTG 
CCCATGCATT GTTTGTGCCG AGATCTAGAA CTTGACTGTG ATGAAGCCAA TTTACGAGCT 
GTCCCATCAG TTTCTTCCAA TGTGACTGTA ATGTCCCTGC AGAGGAACTT CATAAGAACA 
CTCCCTCCCA ACGGCTTCAG GAAGTACCAT GAGCTGCAGA AGCTGTGCCT GCAAAACAAT 
AGGATTCACT CCGTGAGCGT CTCTGCCTTC AGAGGACTGC GCAGCCTCAC TAAACTGTAC 
CTCAGTCATA ACAGAATAAC CTTCCTAAAG CCAGGAGTTT TTGAAGATCT CCACAGACTG 
GAATGGCTGA TAATTGAAGA CAATCACCTC AGTCGAATTT CCCCACTCAC ATTTTACGGA 
CTAAATTCTC TTATTCTCTT AGTGCTGATG AATAATGCCC TCACCCGTTT GCCTGACAAA 
CCCCTCTGTC AGCACATGCC CCGCCTGCAC TGGCTGGACT TTGAAGGCAA CCGTATCCAT 
AATTTAAGAA ATTTGACTTT TATTTCCTGC AATAATTTAA CTGTTTTGGT AATGAGGAAG 
AACAAAATCA ATTACTTAAA TGAACATGCA TTTACACACC TCCAGAAACT AGATGAATTG 
GATTTAGGAA GTAATAAGAT TGAAAATCTT CCACCGAACA TATTTAAGGA TCTGAAGGAG 
CTGTCGCAAT TGAATATTTC TTATAACCCA ATCCAGAAAA TTGAAGTAAA TCAATTCGAT 
TGTCTTGCCA AACTCAAGTC TCTCAGCCTT GAAGGAATAG AAATTTCAAA TATCCAACAA 
AGGATGTTTA GACCGCTCAT AAATCTCTCT CACATCTATT TTAAGAAATT TCAGTACTGT 
GGATACGCAC CACATGTGCG CAGCTGTAAG CCCAACACGG ATGGGATTTC ATCTCTAGAG 
AACCTCTTGG CAAGCATCAT CCAGAGAGTC TTTGTCTGGG TTGTATCTGC CATTACCTGC 
TTTGGAAACA TTTTTGTTAT TTGTATGCGA CCATACATCA GATCTGAGAA CAAGCTGCAT 
GCCATGTCGA TCATTTCTCT CTGCTGTGCT GACTGCCTAA TGGGGGTGTA TCTATTTGTG 
ATTGGTGCCT TTGACCTCAA GTTTCGGGGA GAGTACAATA AGCACGCACA GCCGTGGATG 
GAGAGTGTTC ATTGTCAGTT CATGGGGTCG TTGGCCATTC TGTCCACAGA AGTATCCGTT 
TTACTTTTAA CATTTCTGAC ATTGGAAAAG TACATCTGCA TTGTGTATCC TTTTCGGTGT 
TTAAGGCCTC GAAAATGCAG AACAATCACG GTTCTGATTT TCATTTGGAT TATTGGGTTT 
ATAGTGGCTT TTGCTCCACT TGGCAATAAG GAATTTTTCA AAAACTACTA TGGCACCAAT 
GGAGTATGCT TTCCTCTTCA TTCAGAAGAC ACGGGAAGTA CTGGAGCCCA GATTTATTCA 
GTGGTAATTT TTCTTGGTAT TAACCTGGTG GCATTTATCA TCATTGTGTT CTCCTATGGA 
AGCATGTTTT ACAGTGTTCA TCAAAGCTCC GTAACAGTAA CCGAAATACA GAAGCAAGTG 
AAGAAGGAGG TGGTTCTCGC CAAACGCTTT TTCTTTATCG TTTTCACCGA CGCGCTGTGC 
TGGATTCCCA TCTTCATACT GAAATTTCTT TCACTGCTTC AGGTGGAAAT ACCAGATTCT 
ATTACCTCTT GGGTGGTGAT TTTTATTCTG CCTATCAACA GTGCTTTGAA CCCAATTATC 
TACACGTTGA CCACTAGACC TTTCAAGGAA ATGATCCATC AACTCTGGCA CAACTACAGA 
CAAAGAAGGT CTGTTGACAG GAAAGAGACT CAGAAGGCAT ATGCTCCATC ATTCATCTGG 
GTGGAAATGT GGCCCTTGCA GGAGATGTCC TCTGGGTTCA TGAAGCCAGG CGCTTTCACA 
GACCCCTGTG ATCTGTCGCT AGTTTCTCAG TCATCTAGAC TCAATTCTTA TTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rxfp1 ( XM_030252661.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108521.1 Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu32020
Clone ID Related Accession (Same CDS sequence)	XM_030252661.1 , XM_006501630.2
Accession Version	XM_030252661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	relaxin receptor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCCC TGCAGAGGAA CTTCATAAGA ACACTCCCTC CCAACGGCTT CAGGAAGTAC 
CATGAGCTGC AGAAGCTGTG CCTGCAAAAC AATAGGATTC ACTCCGTGAG CGTCTCTGCC 
TTCAGAGGAC TGCGCAGCCT CACTAAACTG TACCTCAGTC ATAACAGAAT AACCTTCCTA 
AAGCCAGGAG TTTTTGAAGA TCTCCACAGA CTGGAATGGC TGATAATTGA AGACAATCAC 
CTCAGTCGAA TTTCCCCACT CACATTTTAC GGACTAAATT CTCTTATTCT CTTAGTGCTG 
ATGAATAATG CCCTCACCCG TTTGCCTGAC AAACCCCTCT GTCAGCACAT GCCCCGCCTG 
CACTGGCTGG ACTTTGAAGG CAACCGTATC CATAATTTAA GAAATTTGAC TTTTATTTCC 
TGCAATAATT TAACTGTTTT GGTAATGAGG AAGAACAAAA TCAATTACTT AAATGAACAT 
GCATTTACAC ACCTCCAGAA ACTAGATGAA TTGGATTTAG GAAGTAATAA GATTGAAAAT 
CTTCCACCGA ACATATTTAA GGATCTGAAG GAGCTGTCGC AATTGAATAT TTCTTATAAC 
CCAATCCAGA AAATTGAAGT AAATCAATTC GATTGTCTTG CCAAACTCAA GTCTCTCAGC 
CTTGAAGGAA TAGAAATTTC AAATATCCAA CAAAGGATGT TTAGACCGCT CATAAATCTC 
TCTCACATCT ATTTTAAGAA ATTTCAGTAC TGTGGATACG CACCACATGT GCGCAGCTGT 
AAGCCCAACA CGGATGGGAT TTCATCTCTA GAGAACCTCT TGGCAAGCAT CATCCAGAGA 
GTCTTTGTCT GGGTTGTATC TGCCATTACC TGCTTTGGAA ACATTTTTGT TATTTGTATG 
CGACCATACA TCAGATCTGA GAACAAGCTG CATGCCATGT CGATCATTTC TCTCTGCTGT 
GCTGACTGCC TAATGGGGGT GTATCTATTT GTGATTGGTG CCTTTGACCT CAAGTTTCGG 
GGAGAGTACA ATAAGCACGC ACAGCCGTGG ATGGAGAGTG TTCATTGTCA GTTCATGGGG 
TCGTTGGCCA TTCTGTCCAC AGAAGTATCC GTTTTACTTT TAACATTTCT GACATTGGAA 
AAGTACATCT GCATTGTGTA TCCTTTTCGG TGTTTAAGGC CTCGAAAATG CAGAACAATC 
ACGGTTCTGA TTTTCATTTG GATTATTGGG TTTATAGTGG CTTTTGCTCC ACTTGGCAAT 
AAGGAATTTT TCAAAAACTA CTATGGCACC AATGGAGTAT GCTTTCCTCT TCATTCAGAA 
GACACGGGAA GTACTGGAGC CCAGATTTAT TCAGTGGTAA TTTTTCTTGG TATTAACCTG 
GTGGCATTTA TCATCATTGT GTTCTCCTAT GGAAGCATGT TTTACAGTGT TCATCAAAGC 
TCCGTAACAG TAACCGAAAT ACAGAAGCAA GTGAAGAAGG AGGTGGTTCT CGCCAAACGC 
TTTTTCTTTA TCGTTTTCAC CGACGCGCTG TGCTGGATTC CCATCTTCAT ACTGAAATTT 
CTTTCACTGC TTCAGGTGGA AATACCAGAT TCTATTACCT CTTGGGTGGT GATTTTTATT 
CTGCCTATCA ACAGTGCTTT GAACCCAATT ATCTACACGT TGACCACTAG ACCTTTCAAG 
GAAATGATCC ATCAACTCTG GCACAACTAC AGACAAAGAA GGTCTGTTGA CAGGAAAGAG 
ACTCAGAAGG CATATGCTCC ATCATTCATC TGGGTGGAAA TGTGGCCCTT GCAGGAGATG 
TCCTCTGGGT TCATGAAGCC AGGCGCTTTC ACAGACCCCT GTGATCTGTC GCTAGTTTCT 
CAGTCATCTA GACTCAATTC TTATTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108521.1
CDS	439..2328
Translation	MLSLQRNFIRTLPPNGFRKYHELQKLCLQNNRIHSVSVSAFRGLRSLTKLYLSHNRITFLKPGVFEDLHRLEWLIIEDNHLSRISPLTFYGLNSLILLVLMNNALTRLPDKPLCQHMPRLHWLDFEGNRIHNLRNLTFISCNNLTVLVMRKNKINYLNEHAFTHLQKLDELDLGSNKIENLPPNIFKDLKELSQLNISYNPIQKIEVNQFDCLAKLKSLSLEGIEISNIQQRMFRPLINLSHIYFKKFQYCGYAPHVRSCKPNTDGISSLENLLASIIQRVFVWVVSAITCFGNIFVICMRPYIRSENKLHAMSIISLCCADCLMGVYLFVIGAFDLKFRGEYNKHAQPWMESVHCQFMGSLAILSTEVSVLLLTFLTLEKYICIVYPFRCLRPRKCRTITVLIFIWIIGFIVAFAPLGNKEFFKNYYGTNGVCFPLHSEDTGSTGAQIYSVVIFLGINLVAFIIIVFSYGSMFYSVHQSSVTVTEIQKQVKKEVVLAKRFFFIVFTDALCWIPIFILKFLSLLQVEIPDSITSWVVIFILPINSALNPIIYTLTTRPFKEMIHQLWHNYRQRRSVDRKETQKAYAPSFIWVEMWPLQEMSSGFMKPGAFTDPCDLSLVSQSSRLNSYS

Target ORF information:

RefSeq Version	XM_030252661.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252661.1

ORF Insert Sequence:

ATGCTGTCCC TGCAGAGGAA CTTCATAAGA ACACTCCCTC CCAACGGCTT CAGGAAGTAC 
CATGAGCTGC AGAAGCTGTG CCTGCAAAAC AATAGGATTC ACTCCGTGAG CGTCTCTGCC 
TTCAGAGGAC TGCGCAGCCT CACTAAACTG TACCTCAGTC ATAACAGAAT AACCTTCCTA 
AAGCCAGGAG TTTTTGAAGA TCTCCACAGA CTGGAATGGC TGATAATTGA AGACAATCAC 
CTCAGTCGAA TTTCCCCACT CACATTTTAC GGACTAAATT CTCTTATTCT CTTAGTGCTG 
ATGAATAATG CCCTCACCCG TTTGCCTGAC AAACCCCTCT GTCAGCACAT GCCCCGCCTG 
CACTGGCTGG ACTTTGAAGG CAACCGTATC CATAATTTAA GAAATTTGAC TTTTATTTCC 
TGCAATAATT TAACTGTTTT GGTAATGAGG AAGAACAAAA TCAATTACTT AAATGAACAT 
GCATTTACAC ACCTCCAGAA ACTAGATGAA TTGGATTTAG GAAGTAATAA GATTGAAAAT 
CTTCCACCGA ACATATTTAA GGATCTGAAG GAGCTGTCGC AATTGAATAT TTCTTATAAC 
CCAATCCAGA AAATTGAAGT AAATCAATTC GATTGTCTTG CCAAACTCAA GTCTCTCAGC 
CTTGAAGGAA TAGAAATTTC AAATATCCAA CAAAGGATGT TTAGACCGCT CATAAATCTC 
TCTCACATCT ATTTTAAGAA ATTTCAGTAC TGTGGATACG CACCACATGT GCGCAGCTGT 
AAGCCCAACA CGGATGGGAT TTCATCTCTA GAGAACCTCT TGGCAAGCAT CATCCAGAGA 
GTCTTTGTCT GGGTTGTATC TGCCATTACC TGCTTTGGAA ACATTTTTGT TATTTGTATG 
CGACCATACA TCAGATCTGA GAACAAGCTG CATGCCATGT CGATCATTTC TCTCTGCTGT 
GCTGACTGCC TAATGGGGGT GTATCTATTT GTGATTGGTG CCTTTGACCT CAAGTTTCGG 
GGAGAGTACA ATAAGCACGC ACAGCCGTGG ATGGAGAGTG TTCATTGTCA GTTCATGGGG 
TCGTTGGCCA TTCTGTCCAC AGAAGTATCC GTTTTACTTT TAACATTTCT GACATTGGAA 
AAGTACATCT GCATTGTGTA TCCTTTTCGG TGTTTAAGGC CTCGAAAATG CAGAACAATC 
ACGGTTCTGA TTTTCATTTG GATTATTGGG TTTATAGTGG CTTTTGCTCC ACTTGGCAAT 
AAGGAATTTT TCAAAAACTA CTATGGCACC AATGGAGTAT GCTTTCCTCT TCATTCAGAA 
GACACGGGAA GTACTGGAGC CCAGATTTAT TCAGTGGTAA TTTTTCTTGG TATTAACCTG 
GTGGCATTTA TCATCATTGT GTTCTCCTAT GGAAGCATGT TTTACAGTGT TCATCAAAGC 
TCCGTAACAG TAACCGAAAT ACAGAAGCAA GTGAAGAAGG AGGTGGTTCT CGCCAAACGC 
TTTTTCTTTA TCGTTTTCAC CGACGCGCTG TGCTGGATTC CCATCTTCAT ACTGAAATTT 
CTTTCACTGC TTCAGGTGGA AATACCAGAT TCTATTACCT CTTGGGTGGT GATTTTTATT 
CTGCCTATCA ACAGTGCTTT GAACCCAATT ATCTACACGT TGACCACTAG ACCTTTCAAG 
GAAATGATCC ATCAACTCTG GCACAACTAC AGACAAAGAA GGTCTGTTGA CAGGAAAGAG 
ACTCAGAAGG CATATGCTCC ATCATTCATC TGGGTGGAAA TGTGGCCCTT GCAGGAGATG 
TCCTCTGGGT TCATGAAGCC AGGCGCTTTC ACAGACCCCT GTGATCTGTC GCTAGTTTCT 
CAGTCATCTA GACTCAATTC TTATTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rxfp1 ( XM_030252662.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108522.1 Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), transcript variant X3, mRNA.

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CloneID	OMu88247
Clone ID Related Accession (Same CDS sequence)	XM_030252662.1
Accession Version	XM_030252662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	relaxin receptor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATG CCCTCACCCG TTTGCCTGAC AAACCCCTCT GTCAGCACAT GCCCCGCCTG 
CACTGGCTGG ACTTTGAAGG CAACCGTATC CATAATTTAA GAAATTTGAC TTTTATTTCC 
TGCAATAATT TAACTGTTTT GGTAATGAGG AAGAACAAAA TCAATTACTT AAATGAACAT 
GCATTTACAC ACCTCCAGAA ACTAGATGAA TTGGATTTAG GAAGTAATAA GATTGAAAAT 
CTTCCACCGA ACATATTTAA GGATCTGAAG GAGCTGTCGC AATTGAATAT TTCTTATAAC 
CCAATCCAGA AAATTGAAGT AAATCAATTC GATTGTCTTG CCAAACTCAA GTCTCTCAGC 
CTTGAAGGAA TAGAAATTTC AAATATCCAA CAAAGGATGT TTAGACCGCT CATAAATCTC 
TCTCACATCT ATTTTAAGAA ATTTCAGTAC TGTGGATACG CACCACATGT GCGCAGCTGT 
AAGCCCAACA CGGATGGGAT TTCATCTCTA GAGAACCTCT TGGCAAGCAT CATCCAGAGA 
GTCTTTGTCT GGGTTGTATC TGCCATTACC TGCTTTGGAA ACATTTTTGT TATTTGTATG 
CGACCATACA TCAGATCTGA GAACAAGCTG CATGCCATGT CGATCATTTC TCTCTGCTGT 
GCTGACTGCC TAATGGGGGT GTATCTATTT GTGATTGGTG CCTTTGACCT CAAGTTTCGG 
GGAGAGTACA ATAAGCACGC ACAGCCGTGG ATGGAGAGTG TTCATTGTCA GTTCATGGGG 
TCGTTGGCCA TTCTGTCCAC AGAAGTATCC GTTTTACTTT TAACATTTCT GACATTGGAA 
AAGTACATCT GCATTGTGTA TCCTTTTCGG TGTTTAAGGC CTCGAAAATG CAGAACAATC 
ACGGTTCTGA TTTTCATTTG GATTATTGGG TTTATAGTGG CTTTTGCTCC ACTTGGCAAT 
AAGGAATTTT TCAAAAACTA CTATGGCACC AATGGAGTAT GCTTTCCTCT TCATTCAGAA 
GACACGGGAA GTACTGGAGC CCAGATTTAT TCAGTGGTAA TTTTTCTTGG TATTAACCTG 
GTGGCATTTA TCATCATTGT GTTCTCCTAT GGAAGCATGT TTTACAGTGT TCATCAAAGC 
TCCGTAACAG TAACCGAAAT ACAGAAGCAA GTGAAGAAGG AGGTGGTTCT CGCCAAACGC 
TTTTTCTTTA TCGTTTTCAC CGACGCGCTG TGCTGGATTC CCATCTTCAT ACTGAAATTT 
CTTTCACTGC TTCAGGTGGA AATACCAGAT TCTATTACCT CTTGGGTGGT GATTTTTATT 
CTGCCTATCA ACAGTGCTTT GAACCCAATT ATCTACACGT TGACCACTAG ACCTTTCAAG 
GAAATGATCC ATCAACTCTG GCACAACTAC AGACAAAGAA GGTCTGTTGA CAGGAAAGAG 
ACTCAGAAGG CATATGCTCC ATCATTCATC TGGGTGGAAA TGTGGCCCTT GCAGGAGATG 
TCCTCTGGGT TCATGAAGCC AGGCGCTTTC ACAGACCCCT GTGATCTGTC GCTAGTTTCT 
CAGTCATCTA GACTCAATTC TTATTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108522.1
CDS	937..2526
Translation	MNNALTRLPDKPLCQHMPRLHWLDFEGNRIHNLRNLTFISCNNLTVLVMRKNKINYLNEHAFTHLQKLDELDLGSNKIENLPPNIFKDLKELSQLNISYNPIQKIEVNQFDCLAKLKSLSLEGIEISNIQQRMFRPLINLSHIYFKKFQYCGYAPHVRSCKPNTDGISSLENLLASIIQRVFVWVVSAITCFGNIFVICMRPYIRSENKLHAMSIISLCCADCLMGVYLFVIGAFDLKFRGEYNKHAQPWMESVHCQFMGSLAILSTEVSVLLLTFLTLEKYICIVYPFRCLRPRKCRTITVLIFIWIIGFIVAFAPLGNKEFFKNYYGTNGVCFPLHSEDTGSTGAQIYSVVIFLGINLVAFIIIVFSYGSMFYSVHQSSVTVTEIQKQVKKEVVLAKRFFFIVFTDALCWIPIFILKFLSLLQVEIPDSITSWVVIFILPINSALNPIIYTLTTRPFKEMIHQLWHNYRQRRSVDRKETQKAYAPSFIWVEMWPLQEMSSGFMKPGAFTDPCDLSLVSQSSRLNSYS

Target ORF information:

RefSeq Version	XM_030252662.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus relaxin/insulin-like family peptide receptor 1 (Rxfp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252662.1

ORF Insert Sequence:

ATGAATAATG CCCTCACCCG TTTGCCTGAC AAACCCCTCT GTCAGCACAT GCCCCGCCTG 
CACTGGCTGG ACTTTGAAGG CAACCGTATC CATAATTTAA GAAATTTGAC TTTTATTTCC 
TGCAATAATT TAACTGTTTT GGTAATGAGG AAGAACAAAA TCAATTACTT AAATGAACAT 
GCATTTACAC ACCTCCAGAA ACTAGATGAA TTGGATTTAG GAAGTAATAA GATTGAAAAT 
CTTCCACCGA ACATATTTAA GGATCTGAAG GAGCTGTCGC AATTGAATAT TTCTTATAAC 
CCAATCCAGA AAATTGAAGT AAATCAATTC GATTGTCTTG CCAAACTCAA GTCTCTCAGC 
CTTGAAGGAA TAGAAATTTC AAATATCCAA CAAAGGATGT TTAGACCGCT CATAAATCTC 
TCTCACATCT ATTTTAAGAA ATTTCAGTAC TGTGGATACG CACCACATGT GCGCAGCTGT 
AAGCCCAACA CGGATGGGAT TTCATCTCTA GAGAACCTCT TGGCAAGCAT CATCCAGAGA 
GTCTTTGTCT GGGTTGTATC TGCCATTACC TGCTTTGGAA ACATTTTTGT TATTTGTATG 
CGACCATACA TCAGATCTGA GAACAAGCTG CATGCCATGT CGATCATTTC TCTCTGCTGT 
GCTGACTGCC TAATGGGGGT GTATCTATTT GTGATTGGTG CCTTTGACCT CAAGTTTCGG 
GGAGAGTACA ATAAGCACGC ACAGCCGTGG ATGGAGAGTG TTCATTGTCA GTTCATGGGG 
TCGTTGGCCA TTCTGTCCAC AGAAGTATCC GTTTTACTTT TAACATTTCT GACATTGGAA 
AAGTACATCT GCATTGTGTA TCCTTTTCGG TGTTTAAGGC CTCGAAAATG CAGAACAATC 
ACGGTTCTGA TTTTCATTTG GATTATTGGG TTTATAGTGG CTTTTGCTCC ACTTGGCAAT 
AAGGAATTTT TCAAAAACTA CTATGGCACC AATGGAGTAT GCTTTCCTCT TCATTCAGAA 
GACACGGGAA GTACTGGAGC CCAGATTTAT TCAGTGGTAA TTTTTCTTGG TATTAACCTG 
GTGGCATTTA TCATCATTGT GTTCTCCTAT GGAAGCATGT TTTACAGTGT TCATCAAAGC 
TCCGTAACAG TAACCGAAAT ACAGAAGCAA GTGAAGAAGG AGGTGGTTCT CGCCAAACGC 
TTTTTCTTTA TCGTTTTCAC CGACGCGCTG TGCTGGATTC CCATCTTCAT ACTGAAATTT 
CTTTCACTGC TTCAGGTGGA AATACCAGAT TCTATTACCT CTTGGGTGGT GATTTTTATT 
CTGCCTATCA ACAGTGCTTT GAACCCAATT ATCTACACGT TGACCACTAG ACCTTTCAAG 
GAAATGATCC ATCAACTCTG GCACAACTAC AGACAAAGAA GGTCTGTTGA CAGGAAAGAG 
ACTCAGAAGG CATATGCTCC ATCATTCATC TGGGTGGAAA TGTGGCCCTT GCAGGAGATG 
TCCTCTGGGT TCATGAAGCC AGGCGCTTTC ACAGACCCCT GTGATCTGTC GCTAGTTTCT 
CAGTCATCTA GACTCAATTC TTATTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Relaxin induces up-regulation of ADAM10 metalloprotease in RXFP1-expressing cells by PI3K/AKT signaling.
Molecular and cellular endocrinology(2017 Nov)
Boccalini G,Sassoli C,Bani D,Nistri S

Relaxin family peptides: structure-activity relationship studies.
British journal of pharmacology174(10)950-961(2017 May)
Patil NA,Rosengren KJ,Separovic F,Wade JD,Bathgate RAD,Hossain MA

Relaxin deficiency results in increased expression of angiogenesis- and remodelling-related genes in the uterus of early pregnant mice but does not affect endometrial angiogenesis prior to implantation.
Reproductive biology and endocrinology : RB&E1411(2016 Mar)
Marshall SA,Ng L,Unemori EN,Girling JE,Parry LJ

Relaxin Affects Smooth Muscle Biophysical Properties and Mechanical Activity of the Female Mouse Colon.
Endocrinology156(12)4398-410(2015 Dec)
Squecco R,Garella R,Idrizaj E,Nistri S,Francini F,Baccari MC

In a Class of Their Own - RXFP1 and RXFP2 are Unique Members of the LGR Family.
Frontiers in endocrinology6137(2015)
Petrie EJ,Lagaida S,Sethi A,Bathgate RA,Gooley PR

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Rxfp1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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