Slc47a2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc47a2
Entrez Gene ID	380701
Full Name	solute carrier family 47, member 2
Synonyms	4933429E10Rik
General protein information	Preferred Names solute carrier family 47, member 2 Names multidrug and toxin extrusion protein 2 MATE-2 h(+)/organic cation antiporter kidney-specific mMATE-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006533616.1	XP_006533679.1	multidrug and toxin extrusion protein 2 isoform X1
NM_001033542.2	NP_001028714.1	multidrug and toxin extrusion protein 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Slc47a2	NP_001028714.1
Gallus gallus (chicken)	SLC47A1	NP_001025891.2
Danio rerio (zebrafish)	mate4	XP_688576.2
Eremothecium gossypii	AGOS_AER234W	NP_985091.2
Magnaporthe oryzae (rice blast fungus)	MGG_03123	XP_003716878.1
Neurospora crassa	NCU00755	XP_964152.1
Canis lupus familiaris (dog)	LOC100688308	XP_003434650.1
Saccharomyces cerevisiae (baker's yeast)	ERC1	NP_011897.1
Kluyveromyces lactis	KLLA0F21802g	XP_456053.1
Danio rerio (zebrafish)	slc47a2	NP_001073648.1
Arabidopsis thaliana (thale cress)	AT5G19700	NP_197471.1
Rattus norvegicus (Norway rat)	Slc47a2	NP_001178849.1
Danio rerio (zebrafish)	LOC101886448	XP_005157715.1
Arabidopsis thaliana (thale cress)	AT4G29140	NP_194643.1
Xenopus tropicalis (tropical clawed frog)	slc47a2	NP_001096440.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Heterozygous variants of multidrug and toxin extrusions (MATE1 and MATE2-K) have little influence on the disposition of metformin in diabetic patients.
Toyama K, Yonezawa A, Tsuda M, Masuda S, Yano I, Terada T, Osawa R, Katsura T, Hosokawa M, Fujimoto S, Inagaki N, Inui K
Pharmacogenetics and genomics20(2)135-8(2010 Feb)

Tissue distribution, ontogeny and induction of the transporters Multidrug and toxin extrusion (MATE) 1 and MATE2 mRNA expression levels in mice.
Lickteig AJ, Cheng X, Augustine LM, Klaassen CD, Cherrington NJ
Life sciences83(1-2)59-64(2008 Jul)

Functional characterization of testis-specific rodent multidrug and toxic compound extrusion 2, a class III MATE-type polyspecific H+/organic cation exporter.
Hiasa M, Matsumoto T, Komatsu T, Omote H, Moriyama Y
American journal of physiology. Cell physiology293(5)C1437-44(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The purpose of this study was to evaluate the effects of heterozygous MATE variants on the disposition of metformin in mice and humans.
Title: Heterozygous variants of multidrug and toxin extrusions (MATE1 and MATE2-K) have little influence on the disposition of metformin in diabetic patients.

MATE2 mRNA expression levels were the highest in the testis, where high expression was localized to Sertoli cells, a critical cell type of the blood testis barrier.
Title: Tissue distribution, ontogeny and induction of the transporters Multidrug and toxin extrusion (MATE) 1 and MATE2 mRNA expression levels in mice.

testes-specific multidrug and toxic compound extrusion 2 (MATE2) act as a polyspecific H(+)/organic cation exporter in Leydig cells.
Title: Functional characterization of testis-specific rodent multidrug and toxic compound extrusion 2, a class III MATE-type polyspecific H+/organic cation exporter.

The following Slc47a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc47a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12722	NM_001033542.2 Latest version!	Mus musculus solute carrier family 47, member 2 (Slc47a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu46522	XM_006533616.1 Latest version!	Mus musculus solute carrier family 47, member 2 (Slc47a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc47a2 ( NM_001033542.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028714.1 Mus musculus solute carrier family 47, member 2 (Slc47a2), mRNA.

Price & Availability↑

CloneID	OMu12722
Clone ID Related Accession (Same CDS sequence)	NM_001033542.2
Accession Version	NM_001033542.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	multidrug and toxin extrusion protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK133432.1. On Apr 5, 2007 this sequence version replaced NM_001033542.1. ##Evidence-Data-START## Transcript exon combination :: AK133432.1, BC147619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCGG CCGAGGACAG CCTGGGAGCC ACCATCCAGC CCCCGGAGCT GGTCCGCGTC 
CCACGGGGGC GCAGTCTGAG GATCCTGCTG GGCCTTCGAG GAGCGCTGTC CCCCGACGTG 
AGACGGGAGG CGGCCGCGCT AGTGGCGCTC GCGGGCCCGG TGTTCCTGGC GCAGCTGATG 
ATCTTTCTCA TCAGCATCGT CAGTTCCATC TTCTGCGGAC ACCTGGGCAA AGTCGAGTTG 
GACGCCGTGA CACTCGCTGT TTCCGTTGTG AATGTCACTG GAATTTCAGT AGGGACTGGC 
TTGGCCTCGG CTTGTGACAC ACTGATGTCT CAGTCCTTTG GAGGCAAGAA CTTGAAGCGC 
GTGGGGGTCA TACTCCAAAG GGGCATCCTC ATCCTGCTGC TCTGCTGCTT CCCATGCTGG 
GCCATCTTCC TCAACACCGA GCGCCTGCTG CTGCTCCTAA GGCAGGACCC GGATGTCGCC 
AGGCTAGCCC AGGTTTATGT GATGATATGC ATCCCTGCTC TTCCTGCAGC GTTCCTATTC 
CAGCTGCAGA CGCGGTATTT ACAGAGCCAG GGCATCATTA TGCCCCAAGT TATTGTTGGG 
ATCGCAGCAA ATGTCGTCAA CGTGGGCATG AACGCCTTCT TGCTGTATGC CTTGGACCTC 
GGAGTGGTAG GGTCGGCCTG GGCCAACACC ACCTCTCAGT TCTTCCTGTC TGCCCTGCTT 
TTCCTCTACG TGTGGTGGAA GAGAATCCAC ATCCACACTT GGGGAGGGTG GACAAGGGAG 
TGCTTCCAGG AGTGGAGCTC CTACACGCGC CTGGCCATCC CCAGTATGTT CATGGTGTGC 
ATCGAGTGGT GGACCTTCGA GATTGGGACC TTCCTTGCAG GCCTTGTTAA TGTGACAGAA 
CTGGGAGCCC AGGCCGTCAT CTATGAACTG GCTTCTGTCG CCTACATGGT GCCCTTTGGC 
TTTGGTGTGG CTGCCAGCGT CCGAGTGGGC AATGCTCTGG GGGCAGGGAA CGCTGACCAG 
GCCCGGTGTT CCTGTACCAC TGTTCTTCTG TGTGCTGGTG TCTGTGCACT CCTGGTGGGG 
ATTCTGCTCG CAGCCTTGAA GGATGTGGTT GCCTACATCT TCACCAATGA CAAGGACATC 
ATTTCCCTTG TGAGTCAAGT GATGCCCATT TTTGCTCCCT TTCATCTCTT TGATGCACTT 
GCGGGCACCT GTGGCGGGGT CCTGAGAGGC ACGGGAAAAC AAAAGATAGG CGCTGTCCTG 
AACACTATCG GTTACTACGG GTTTGGTTTT CCTATTGGAG TGTCCCTGAT GTTTGCTGCT 
AAGCTTGGGA TAATAGGCCT CTGGGCTGGA CTGATAGTCT GTGTCTCCTT CCAAGCCTTC 
TCCTATTTAA TCTACATCCT GAGGACAAAC TGGAGCAGAG TCGCAGAGCA GGCACAAGTT 
CGAGCTGGGC TAAAAAGCAC CAAGGAGTTG ATACCCACGC CAGCAGATCT GCCCATCTTG 
GAAAGAGAAG TTATGGATGG AGTGATTTTG CCTGATATCA TCAGGCCAGA GAGTCAGACC 
GGCCAGCTGG TGGTGGAAGA GAACAGCCAG TGTGCAGTGC CCACCGTTGG GGAGGTCTTG 
ACGGGGAGAC AGCTGGTTTT CTATCGTGGG ATGGCTCTGA CTGTTTCTGT TGCTGTCCTC 
ATAGCAGGGA TTGTGGTGCG AGTTTTCAAT GACCGTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028714.1
CDS	83..1804
Translation	MEPAEDSLGATIQPPELVRVPRGRSLRILLGLRGALSPDVRREAAALVALAGPVFLAQLMIFLISIVSSIFCGHLGKVELDAVTLAVSVVNVTGISVGTGLASACDTLMSQSFGGKNLKRVGVILQRGILILLLCCFPCWAIFLNTERLLLLLRQDPDVARLAQVYVMICIPALPAAFLFQLQTRYLQSQGIIMPQVIVGIAANVVNVGMNAFLLYALDLGVVGSAWANTTSQFFLSALLFLYVWWKRIHIHTWGGWTRECFQEWSSYTRLAIPSMFMVCIEWWTFEIGTFLAGLVNVTELGAQAVIYELASVAYMVPFGFGVAASVRVGNALGAGNADQARCSCTTVLLCAGVCALLVGILLAALKDVVAYIFTNDKDIISLVSQVMPIFAPFHLFDALAGTCGGVLRGTGKQKIGAVLNTIGYYGFGFPIGVSLMFAAKLGIIGLWAGLIVCVSFQAFSYLIYILRTNWSRVAEQAQVRAGLKSTKELIPTPADLPILEREVMDGVILPDIIRPESQTGQLVVEENSQCAVPTVGEVLTGRQLVFYRGMALTVSVAVLIAGIVVRVFNDRG

Target ORF information:

RefSeq Version	NM_001033542.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 47, member 2 (Slc47a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033542.2

ORF Insert Sequence:

ATGGAGCCGG CCGAGGACAG CCTGGGAGCC ACCATCCAGC CCCCGGAGCT GGTCCGCGTC 
CCACGGGGGC GCAGTCTGAG GATCCTGCTG GGCCTTCGAG GAGCGCTGTC CCCCGACGTG 
AGACGGGAGG CGGCCGCGCT AGTGGCGCTC GCGGGCCCGG TGTTCCTGGC GCAGCTGATG 
ATCTTTCTCA TCAGCATCGT CAGTTCCATC TTCTGCGGAC ACCTGGGCAA AGTCGAGTTG 
GACGCCGTGA CACTCGCTGT TTCCGTTGTG AATGTCACTG GAATTTCAGT AGGGACTGGC 
TTGGCCTCGG CTTGTGACAC ACTGATGTCT CAGTCCTTTG GAGGCAAGAA CTTGAAGCGC 
GTGGGGGTCA TACTCCAAAG GGGCATCCTC ATCCTGCTGC TCTGCTGCTT CCCATGCTGG 
GCCATCTTCC TCAACACCGA GCGCCTGCTG CTGCTCCTAA GGCAGGACCC GGATGTCGCC 
AGGCTAGCCC AGGTTTATGT GATGATATGC ATCCCTGCTC TTCCTGCAGC GTTCCTATTC 
CAGCTGCAGA CGCGGTATTT ACAGAGCCAG GGCATCATTA TGCCCCAAGT TATTGTTGGG 
ATCGCAGCAA ATGTCGTCAA CGTGGGCATG AACGCCTTCT TGCTGTATGC CTTGGACCTC 
GGAGTGGTAG GGTCGGCCTG GGCCAACACC ACCTCTCAGT TCTTCCTGTC TGCCCTGCTT 
TTCCTCTACG TGTGGTGGAA GAGAATCCAC ATCCACACTT GGGGAGGGTG GACAAGGGAG 
TGCTTCCAGG AGTGGAGCTC CTACACGCGC CTGGCCATCC CCAGTATGTT CATGGTGTGC 
ATCGAGTGGT GGACCTTCGA GATTGGGACC TTCCTTGCAG GCCTTGTTAA TGTGACAGAA 
CTGGGAGCCC AGGCCGTCAT CTATGAACTG GCTTCTGTCG CCTACATGGT GCCCTTTGGC 
TTTGGTGTGG CTGCCAGCGT CCGAGTGGGC AATGCTCTGG GGGCAGGGAA CGCTGACCAG 
GCCCGGTGTT CCTGTACCAC TGTTCTTCTG TGTGCTGGTG TCTGTGCACT CCTGGTGGGG 
ATTCTGCTCG CAGCCTTGAA GGATGTGGTT GCCTACATCT TCACCAATGA CAAGGACATC 
ATTTCCCTTG TGAGTCAAGT GATGCCCATT TTTGCTCCCT TTCATCTCTT TGATGCACTT 
GCGGGCACCT GTGGCGGGGT CCTGAGAGGC ACGGGAAAAC AAAAGATAGG CGCTGTCCTG 
AACACTATCG GTTACTACGG GTTTGGTTTT CCTATTGGAG TGTCCCTGAT GTTTGCTGCT 
AAGCTTGGGA TAATAGGCCT CTGGGCTGGA CTGATAGTCT GTGTCTCCTT CCAAGCCTTC 
TCCTATTTAA TCTACATCCT GAGGACAAAC TGGAGCAGAG TCGCAGAGCA GGCACAAGTT 
CGAGCTGGGC TAAAAAGCAC CAAGGAGTTG ATACCCACGC CAGCAGATCT GCCCATCTTG 
GAAAGAGAAG TTATGGATGG AGTGATTTTG CCTGATATCA TCAGGCCAGA GAGTCAGACC 
GGCCAGCTGG TGGTGGAAGA GAACAGCCAG TGTGCAGTGC CCACCGTTGG GGAGGTCTTG 
ACGGGGAGAC AGCTGGTTTT CTATCGTGGG ATGGCTCTGA CTGTTTCTGT TGCTGTCCTC 
ATAGCAGGGA TTGTGGTGCG AGTTTTCAAT GACCGTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc47a2 ( XM_006533616.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533679.1 Mus musculus solute carrier family 47, member 2 (Slc47a2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46522
Clone ID Related Accession (Same CDS sequence)	XM_006533616.1
Accession Version	XM_006533616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	multidrug and toxin extrusion protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT CCTTTGGAGG CAAGAACTTG AAGCGCGTGG GGGTCATACT CCAAAGGGGC 
ATCCTCATCC TGCTGCTCTG CTGCTTCCCA TGCTGGGCCA TCTTCCTCAA CACCGAGCGC 
CTGCTGCTGC TCCTAAGGCA GGACCCGGAT GTCGCCAGGC TAGCCCAGGT TTATGTGATG 
ATATGCATCC CTGCTCTTCC TGCAGCGTTC CTATTCCAGC TGCAGACGCG GTATTTACAG 
AGCCAGGGCA TCATTATGCC CCAAGTTATT GTTGGGATCG CAGCAAATGT CGTCAACGTG 
GGCATGAACG CCTTCTTGCT GTATGCCTTG GACCTCGGAG TGGTAGGGTC GGCCTGGGCC 
AACACCACCT CTCAGTTCTT CCTGTCTGCC CTGCTTTTCC TCTACGTGTG GTGGAAGAGA 
ATCCACATCC ACACTTGGGG AGGGTGGACA AGGGAGTGCT TCCAGGAGTG GAGCTCCTAC 
ACGCGCCTGG CCATCCCCAG TATGTTCATG GTGTGCATCG AGTGGTGGAC CTTCGAGATT 
GGGACCTTCC TTGCAGGCCT TGTTAATGTG ACAGAACTGG GAGCCCAGGC CGTCATCTAT 
GAACTGGCTT CTGTCGCCTA CATGGTGCCC TTTGGCTTTG GTGTGGCTGC CAGCGTCCGA 
GTGGGCAATG CTCTGGGGGC AGGGAACGCT GACCAGGCCC GGTGTTCCTG TACCACTGTT 
CTTCTGTGTG CTGGTGTCTG TGCACTCCTG GTGGGGATTC TGCTCGCAGC CTTGAAGGAT 
GTGGTTGCCT ACATCTTCAC CAATGACAAG GACATCATTT CCCTTGTGAG TCAAGTGATG 
CCCATTTTTG CTCCCTTTCA TCTCTTTGAT GCACTTGCGG GCACCTGTGG CGGGGTCCTG 
AGAGGCACGG GAAAACAAAA GATAGGCGCT GTCCTGAACA CTATCGGTTA CTACGGGTTT 
GGTTTTCCTA TTGGAGTGTC CCTGATGTTT GCTGCTAAGC TTGGGATAAT AGGCCTCTGG 
GCTGGACTGA TAGTCTGTGT CTCCTTCCAA GCCTTCTCCT ATTTAATCTA CATCCTGAGG 
ACAAACTGGA GCAGAGTCGC AGAGCAGGCA CAAGTTCGAG CTGGGCTAAA AAGCACCAAG 
GAGTTGATAC CCACGCCAGC AGATCTGCCC ATCTTGGAAA GAGAAGTTAT GGATGGAGTG 
ATTTTGCCTG ATATCATCAG GCCAGAGAGT CAGACCGGCC AGCTGGTGGT GGAAGAGAAC 
AGCCAGTGTG CAGTGCCCAC CGTTGGGGAG GTCTTGACGG GGAGACAGCT GGTTTTCTAT 
CGTGGGATGG CTCTGACTGT TTCTGTTGCT GTCCTCATAG CAGGGATTGT GGTGCGAGTT 
TTCAATGACC GTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533679.1
CDS	161..1558
Translation	MSQSFGGKNLKRVGVILQRGILILLLCCFPCWAIFLNTERLLLLLRQDPDVARLAQVYVMICIPALPAAFLFQLQTRYLQSQGIIMPQVIVGIAANVVNVGMNAFLLYALDLGVVGSAWANTTSQFFLSALLFLYVWWKRIHIHTWGGWTRECFQEWSSYTRLAIPSMFMVCIEWWTFEIGTFLAGLVNVTELGAQAVIYELASVAYMVPFGFGVAASVRVGNALGAGNADQARCSCTTVLLCAGVCALLVGILLAALKDVVAYIFTNDKDIISLVSQVMPIFAPFHLFDALAGTCGGVLRGTGKQKIGAVLNTIGYYGFGFPIGVSLMFAAKLGIIGLWAGLIVCVSFQAFSYLIYILRTNWSRVAEQAQVRAGLKSTKELIPTPADLPILEREVMDGVILPDIIRPESQTGQLVVEENSQCAVPTVGEVLTGRQLVFYRGMALTVSVAVLIAGIVVRVFNDRG

Target ORF information:

RefSeq Version	XM_006533616.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 47, member 2 (Slc47a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533616.1

ORF Insert Sequence:

ATGTCTCAGT CCTTTGGAGG CAAGAACTTG AAGCGCGTGG GGGTCATACT CCAAAGGGGC 
ATCCTCATCC TGCTGCTCTG CTGCTTCCCA TGCTGGGCCA TCTTCCTCAA CACCGAGCGC 
CTGCTGCTGC TCCTAAGGCA GGACCCGGAT GTCGCCAGGC TAGCCCAGGT TTATGTGATG 
ATATGCATCC CTGCTCTTCC TGCAGCGTTC CTATTCCAGC TGCAGACGCG GTATTTACAG 
AGCCAGGGCA TCATTATGCC CCAAGTTATT GTTGGGATCG CAGCAAATGT CGTCAACGTG 
GGCATGAACG CCTTCTTGCT GTATGCCTTG GACCTCGGAG TGGTAGGGTC GGCCTGGGCC 
AACACCACCT CTCAGTTCTT CCTGTCTGCC CTGCTTTTCC TCTACGTGTG GTGGAAGAGA 
ATCCACATCC ACACTTGGGG AGGGTGGACA AGGGAGTGCT TCCAGGAGTG GAGCTCCTAC 
ACGCGCCTGG CCATCCCCAG TATGTTCATG GTGTGCATCG AGTGGTGGAC CTTCGAGATT 
GGGACCTTCC TTGCAGGCCT TGTTAATGTG ACAGAACTGG GAGCCCAGGC CGTCATCTAT 
GAACTGGCTT CTGTCGCCTA CATGGTGCCC TTTGGCTTTG GTGTGGCTGC CAGCGTCCGA 
GTGGGCAATG CTCTGGGGGC AGGGAACGCT GACCAGGCCC GGTGTTCCTG TACCACTGTT 
CTTCTGTGTG CTGGTGTCTG TGCACTCCTG GTGGGGATTC TGCTCGCAGC CTTGAAGGAT 
GTGGTTGCCT ACATCTTCAC CAATGACAAG GACATCATTT CCCTTGTGAG TCAAGTGATG 
CCCATTTTTG CTCCCTTTCA TCTCTTTGAT GCACTTGCGG GCACCTGTGG CGGGGTCCTG 
AGAGGCACGG GAAAACAAAA GATAGGCGCT GTCCTGAACA CTATCGGTTA CTACGGGTTT 
GGTTTTCCTA TTGGAGTGTC CCTGATGTTT GCTGCTAAGC TTGGGATAAT AGGCCTCTGG 
GCTGGACTGA TAGTCTGTGT CTCCTTCCAA GCCTTCTCCT ATTTAATCTA CATCCTGAGG 
ACAAACTGGA GCAGAGTCGC AGAGCAGGCA CAAGTTCGAG CTGGGCTAAA AAGCACCAAG 
GAGTTGATAC CCACGCCAGC AGATCTGCCC ATCTTGGAAA GAGAAGTTAT GGATGGAGTG 
ATTTTGCCTG ATATCATCAG GCCAGAGAGT CAGACCGGCC AGCTGGTGGT GGAAGAGAAC 
AGCCAGTGTG CAGTGCCCAC CGTTGGGGAG GTCTTGACGG GGAGACAGCT GGTTTTCTAT 
CGTGGGATGG CTCTGACTGT TTCTGTTGCT GTCCTCATAG CAGGGATTGT GGTGCGAGTT 
TTCAATGACC GTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Heterozygous variants of multidrug and toxin extrusions (MATE1 and MATE2-K) have little influence on the disposition of metformin in diabetic patients.
Pharmacogenetics and genomics20(2)135-8(2010 Feb)
Toyama K,Yonezawa A,Tsuda M,Masuda S,Yano I,Terada T,Osawa R,Katsura T,Hosokawa M,Fujimoto S,Inagaki N,Inui K

Tissue distribution, ontogeny and induction of the transporters Multidrug and toxin extrusion (MATE) 1 and MATE2 mRNA expression levels in mice.
Life sciences83(1-2)59-64(2008 Jul)
Lickteig AJ,Cheng X,Augustine LM,Klaassen CD,Cherrington NJ

Functional characterization of testis-specific rodent multidrug and toxic compound extrusion 2, a class III MATE-type polyspecific H+/organic cation exporter.
American journal of physiology. Cell physiology293(5)C1437-44(2007 Nov)
Hiasa M,Matsumoto T,Komatsu T,Omote H,Moriyama Y

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Slc47a2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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