Prune2 cDNA ORF clone, Mus musculus(house mouse)

The following Prune2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prune2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu07977 NM_181348.4
Latest version!
Mus musculus prune homolog 2 (Prune2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55599 XM_006527144.3
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55600 XM_006527145.2
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55601 XM_006527146.2
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55602 XM_006527147.3
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55604 XM_006527149.3
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55606 XM_006527151.3
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55607 XM_006527152.3
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55608 XM_006527153.3
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55609 XM_006527154.3
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55610 XM_030250976.1
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu79979 XM_017318226.2
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu79980 XM_017318227.2
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu79981 XM_017318228.2
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu79982 XM_017318229.2
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu79983 XM_017318230.2
Latest version!
Mus musculus prune homolog 2 (Prune2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OMu55602 XM_006527147.2 Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55601 XM_006527146.1 Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55606 XM_006527151.2 Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55600 XM_006527145.1 Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55609 XM_006527154.2 Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55599 XM_006527144.2 Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu79979 XM_017318226.1 Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu79982 XM_017318229.1 Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55607 XM_006527152.2 Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu79983 XM_017318230.1 Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu79981 XM_017318228.1 Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55604 XM_006527149.2 Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu79980 XM_017318227.1 Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu55608 XM_006527153.2 Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu07977
    Clone ID Related Accession (Same CDS sequence) NM_181348.4
    Accession Version NM_181348.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK039241.1, AC126674.3, BC094224.1 and AC147026.2. On or before Jul 1, 2009 this sequence version replaced XR_035391.1, XR_032740.1, NM_181348.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849390, SAMN01164133 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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    721
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    841
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    961
    1021
    1081
    1141
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    1261
    1321
    1381
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    1501
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    1741
    1801
    1861
    1921
    1981
    2041
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    2161
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    2641
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    4141
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    4381
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    5161
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    5521
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    5881
    5941
    6001
    6061
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    6181
    6241
    6301
    6361
    6421
    6481
    6541
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    7141
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    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA AGTATGATGA AGAGAAATCT
    TTTAAGAGAA GTGTGAGACT GGATGAAGAA CTGAGGGAGG CATCCGAAGC AGCTAAAACT
    AGCTGCCTTT ACAATGATCC AGAAATGTCT TCTATGGAAA AGGACATTGA CATGAAGCTG
    AAGGAGAAGC CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_851993.3
    CDS140..9394
    Translation

    Target ORF information:

    RefSeq Version NM_181348.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_181348.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA AGTATGATGA AGAGAAATCT
    TTTAAGAGAA GTGTGAGACT GGATGAAGAA CTGAGGGAGG CATCCGAAGC AGCTAAAACT
    AGCTGCCTTT ACAATGATCC AGAAATGTCT TCTATGGAAA AGGACATTGA CATGAAGCTG
    AAGGAGAAGC CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55599
    Clone ID Related Accession (Same CDS sequence) XM_006527144.3 , XM_006527144.2
    Accession Version XM_006527144.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9264bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527144.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGACTG GATGAAGAAC TGAGGGAGGC ATCCGAAGCA
    GCTAAAACTA GCTGCCTTTA CAATGATCCA GAAATGTCTT CTATGGAAAA GGACATTGAC
    ATGAAGCTGA AGGAGAAGCC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527207.1
    CDS587..9850
    Translation

    Target ORF information:

    RefSeq Version XM_006527144.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527144.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGACTG GATGAAGAAC TGAGGGAGGC ATCCGAAGCA
    GCTAAAACTA GCTGCCTTTA CAATGATCCA GAAATGTCTT CTATGGAAAA GGACATTGAC
    ATGAAGCTGA AGGAGAAGCC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55599
    Clone ID Related Accession (Same CDS sequence) XM_006527144.3 , XM_006527144.2
    Accession Version XM_006527144.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9264bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGACTG GATGAAGAAC TGAGGGAGGC ATCCGAAGCA
    GCTAAAACTA GCTGCCTTTA CAATGATCCA GAAATGTCTT CTATGGAAAA GGACATTGAC
    ATGAAGCTGA AGGAGAAGCC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527207.1
    CDS586..9849
    Translation

    Target ORF information:

    RefSeq Version XM_006527144.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527144.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
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    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGACTG GATGAAGAAC TGAGGGAGGC ATCCGAAGCA
    GCTAAAACTA GCTGCCTTTA CAATGATCCA GAAATGTCTT CTATGGAAAA GGACATTGAC
    ATGAAGCTGA AGGAGAAGCC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55600
    Clone ID Related Accession (Same CDS sequence) XM_006527145.2 , XM_006527145.1
    Accession Version XM_006527145.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527145.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGATCC CACGGCCAAC
    AAAAGCTCTG GTCAAGAGTC AGAGTCTATT CCAGAATATA CGGCTGAAGA GGAGCGAGAA
    GACAACCGGC TGTGGAGGAC GGTGGTCATC GGGGAGCAGG AGCAACGGAT TGACATGAAG
    GTCATTGAAC CCTACAGGAG AGTCATTTCT CACGGAGGAG ACTCAGGATA CTATGGGGAC
    GGTCTGAATG CCATCATTGT GTTTGCTGCC TGCTTTCTGC CAGACAGCAG CCGGGCGGAT
    TACCACTATG TCATGGAAAA CCTTTTCCTA TATGTAATAA GTACCTTAGA GCTGATGGTT
    GCTGAAGACT ACATGATCGT GTACTTAAAT GGTGCAACCC CAAGGCGGAA GATGCCAGGA
    CTAGGTTGGA TGAAGAAATG CTACCAGATG ATTGACAGAC GGTTGCGCAA GAATTTGAAG
    TCATTCATCA TTGTTCACCC ATCTTGGTTC ATCAGGACAA TCTTAGCAGT GACTCGGCCT
    TTTATAAGCT CTAAATTCAG CAGTAAAATT AAATATGTGA CTAGCTTATC TGAGCTCAGT
    GGCCTGATCC CCATGGACTG CATCCACATT CCAGAGAGCA TCATCAAGTA TGATGAAGAG
    AAATCTTTTA AGAGAAGTGT GAGACTGGAT GAAGAACTGA GGGAGGCATC CGAAGCAGCT
    AAAACTAGCT GCCTTTACAA TGATCCAGAA ATGTCTTCTA TGGAAAAGGA CATTGACATG
    AAGCTGAAGG AGAAGCCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527208.1
    CDS588..9848
    Translation

    Target ORF information:

    RefSeq Version XM_006527145.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527145.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGATCC CACGGCCAAC
    AAAAGCTCTG GTCAAGAGTC AGAGTCTATT CCAGAATATA CGGCTGAAGA GGAGCGAGAA
    GACAACCGGC TGTGGAGGAC GGTGGTCATC GGGGAGCAGG AGCAACGGAT TGACATGAAG
    GTCATTGAAC CCTACAGGAG AGTCATTTCT CACGGAGGAG ACTCAGGATA CTATGGGGAC
    GGTCTGAATG CCATCATTGT GTTTGCTGCC TGCTTTCTGC CAGACAGCAG CCGGGCGGAT
    TACCACTATG TCATGGAAAA CCTTTTCCTA TATGTAATAA GTACCTTAGA GCTGATGGTT
    GCTGAAGACT ACATGATCGT GTACTTAAAT GGTGCAACCC CAAGGCGGAA GATGCCAGGA
    CTAGGTTGGA TGAAGAAATG CTACCAGATG ATTGACAGAC GGTTGCGCAA GAATTTGAAG
    TCATTCATCA TTGTTCACCC ATCTTGGTTC ATCAGGACAA TCTTAGCAGT GACTCGGCCT
    TTTATAAGCT CTAAATTCAG CAGTAAAATT AAATATGTGA CTAGCTTATC TGAGCTCAGT
    GGCCTGATCC CCATGGACTG CATCCACATT CCAGAGAGCA TCATCAAGTA TGATGAAGAG
    AAATCTTTTA AGAGAAGTGT GAGACTGGAT GAAGAACTGA GGGAGGCATC CGAAGCAGCT
    AAAACTAGCT GCCTTTACAA TGATCCAGAA ATGTCTTCTA TGGAAAAGGA CATTGACATG
    AAGCTGAAGG AGAAGCCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55600
    Clone ID Related Accession (Same CDS sequence) XM_006527145.2 , XM_006527145.1
    Accession Version XM_006527145.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGATCC CACGGCCAAC
    AAAAGCTCTG GTCAAGAGTC AGAGTCTATT CCAGAATATA CGGCTGAAGA GGAGCGAGAA
    GACAACCGGC TGTGGAGGAC GGTGGTCATC GGGGAGCAGG AGCAACGGAT TGACATGAAG
    GTCATTGAAC CCTACAGGAG AGTCATTTCT CACGGAGGAG ACTCAGGATA CTATGGGGAC
    GGTCTGAATG CCATCATTGT GTTTGCTGCC TGCTTTCTGC CAGACAGCAG CCGGGCGGAT
    TACCACTATG TCATGGAAAA CCTTTTCCTA TATGTAATAA GTACCTTAGA GCTGATGGTT
    GCTGAAGACT ACATGATCGT GTACTTAAAT GGTGCAACCC CAAGGCGGAA GATGCCAGGA
    CTAGGTTGGA TGAAGAAATG CTACCAGATG ATTGACAGAC GGTTGCGCAA GAATTTGAAG
    TCATTCATCA TTGTTCACCC ATCTTGGTTC ATCAGGACAA TCTTAGCAGT GACTCGGCCT
    TTTATAAGCT CTAAATTCAG CAGTAAAATT AAATATGTGA CTAGCTTATC TGAGCTCAGT
    GGCCTGATCC CCATGGACTG CATCCACATT CCAGAGAGCA TCATCAAGTA TGATGAAGAG
    AAATCTTTTA AGAGAAGTGT GAGACTGGAT GAAGAACTGA GGGAGGCATC CGAAGCAGCT
    AAAACTAGCT GCCTTTACAA TGATCCAGAA ATGTCTTCTA TGGAAAAGGA CATTGACATG
    AAGCTGAAGG AGAAGCCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527208.1
    CDS586..9846
    Translation

    Target ORF information:

    RefSeq Version XM_006527145.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527145.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGATCC CACGGCCAAC
    AAAAGCTCTG GTCAAGAGTC AGAGTCTATT CCAGAATATA CGGCTGAAGA GGAGCGAGAA
    GACAACCGGC TGTGGAGGAC GGTGGTCATC GGGGAGCAGG AGCAACGGAT TGACATGAAG
    GTCATTGAAC CCTACAGGAG AGTCATTTCT CACGGAGGAG ACTCAGGATA CTATGGGGAC
    GGTCTGAATG CCATCATTGT GTTTGCTGCC TGCTTTCTGC CAGACAGCAG CCGGGCGGAT
    TACCACTATG TCATGGAAAA CCTTTTCCTA TATGTAATAA GTACCTTAGA GCTGATGGTT
    GCTGAAGACT ACATGATCGT GTACTTAAAT GGTGCAACCC CAAGGCGGAA GATGCCAGGA
    CTAGGTTGGA TGAAGAAATG CTACCAGATG ATTGACAGAC GGTTGCGCAA GAATTTGAAG
    TCATTCATCA TTGTTCACCC ATCTTGGTTC ATCAGGACAA TCTTAGCAGT GACTCGGCCT
    TTTATAAGCT CTAAATTCAG CAGTAAAATT AAATATGTGA CTAGCTTATC TGAGCTCAGT
    GGCCTGATCC CCATGGACTG CATCCACATT CCAGAGAGCA TCATCAAGTA TGATGAAGAG
    AAATCTTTTA AGAGAAGTGT GAGACTGGAT GAAGAACTGA GGGAGGCATC CGAAGCAGCT
    AAAACTAGCT GCCTTTACAA TGATCCAGAA ATGTCTTCTA TGGAAAAGGA CATTGACATG
    AAGCTGAAGG AGAAGCCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55601
    Clone ID Related Accession (Same CDS sequence) XM_006527146.1 , XM_006527146.2
    Accession Version XM_006527146.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA ACTGGATGAA
    GAACTGAGGG AGGCATCCGA AGCAGCTAAA ACTAGCTGCC TTTACAATGA TCCAGAAATG
    TCTTCTATGG AAAAGGACAT TGACATGAAG CTGAAGGAGA AGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527209.1
    CDS585..9812
    Translation

    Target ORF information:

    RefSeq Version XM_006527146.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527146.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA ACTGGATGAA
    GAACTGAGGG AGGCATCCGA AGCAGCTAAA ACTAGCTGCC TTTACAATGA TCCAGAAATG
    TCTTCTATGG AAAAGGACAT TGACATGAAG CTGAAGGAGA AGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55601
    Clone ID Related Accession (Same CDS sequence) XM_006527146.1 , XM_006527146.2
    Accession Version XM_006527146.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527146.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA ACTGGATGAA
    GAACTGAGGG AGGCATCCGA AGCAGCTAAA ACTAGCTGCC TTTACAATGA TCCAGAAATG
    TCTTCTATGG AAAAGGACAT TGACATGAAG CTGAAGGAGA AGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527209.1
    CDS587..9814
    Translation

    Target ORF information:

    RefSeq Version XM_006527146.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527146.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA ACTGGATGAA
    GAACTGAGGG AGGCATCCGA AGCAGCTAAA ACTAGCTGCC TTTACAATGA TCCAGAAATG
    TCTTCTATGG AAAAGGACAT TGACATGAAG CTGAAGGAGA AGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55602
    Clone ID Related Accession (Same CDS sequence) XM_006527147.2 , XM_006527147.3
    Accession Version XM_006527147.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527147.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGAACT AGCTGCCTTT ACAATGATCC AGAAATGTCT
    TCTATGGAAA AGGACATTGA CATGAAGCTG AAGGAGAAGC CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527210.1
    CDS586..9810
    Translation

    Target ORF information:

    RefSeq Version XM_006527147.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527147.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGAACT AGCTGCCTTT ACAATGATCC AGAAATGTCT
    TCTATGGAAA AGGACATTGA CATGAAGCTG AAGGAGAAGC CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55602
    Clone ID Related Accession (Same CDS sequence) XM_006527147.2 , XM_006527147.3
    Accession Version XM_006527147.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527147.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGAACT AGCTGCCTTT ACAATGATCC AGAAATGTCT
    TCTATGGAAA AGGACATTGA CATGAAGCTG AAGGAGAAGC CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527210.1
    CDS587..9811
    Translation

    Target ORF information:

    RefSeq Version XM_006527147.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527147.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGAACT AGCTGCCTTT ACAATGATCC AGAAATGTCT
    TCTATGGAAA AGGACATTGA CATGAAGCTG AAGGAGAAGC CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55604
    Clone ID Related Accession (Same CDS sequence) XM_006527149.3 , XM_006527149.2
    Accession Version XM_006527149.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527149.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGACTG GATGAAGAAC TGAGGGAGGC ATCCGAAGCA
    GCTAACATTG ACATGAAGCT GAAGGAGAAG CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527212.1
    CDS588..9803
    Translation

    Target ORF information:

    RefSeq Version XM_006527149.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527149.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGACTG GATGAAGAAC TGAGGGAGGC ATCCGAAGCA
    GCTAACATTG ACATGAAGCT GAAGGAGAAG CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55604
    Clone ID Related Accession (Same CDS sequence) XM_006527149.3 , XM_006527149.2
    Accession Version XM_006527149.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527149.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGACTG GATGAAGAAC TGAGGGAGGC ATCCGAAGCA
    GCTAACATTG ACATGAAGCT GAAGGAGAAG CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527212.1
    CDS586..9801
    Translation

    Target ORF information:

    RefSeq Version XM_006527149.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527149.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGACTG GATGAAGAAC TGAGGGAGGC ATCCGAAGCA
    GCTAACATTG ACATGAAGCT GAAGGAGAAG CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55606
    Clone ID Related Accession (Same CDS sequence) XM_006527151.2 , XM_006527151.3
    Accession Version XM_006527151.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527151.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
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    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
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    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    3601
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    3721
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4621
    4681
    4741
    4801
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    4921
    4981
    5041
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    5161
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    5281
    5341
    5401
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    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA ACTGGATGAA
    GAACTGAGGG AGGCATCCGA AGCAGCTAAC ATTGACATGA AGCTGAAGGA GAAGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527214.1
    CDS586..9765
    Translation

    Target ORF information:

    RefSeq Version XM_006527151.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527151.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA ACTGGATGAA
    GAACTGAGGG AGGCATCCGA AGCAGCTAAC ATTGACATGA AGCTGAAGGA GAAGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55606
    Clone ID Related Accession (Same CDS sequence) XM_006527151.2 , XM_006527151.3
    Accession Version XM_006527151.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527151.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA ACTGGATGAA
    GAACTGAGGG AGGCATCCGA AGCAGCTAAC ATTGACATGA AGCTGAAGGA GAAGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527214.1
    CDS587..9766
    Translation

    Target ORF information:

    RefSeq Version XM_006527151.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527151.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA ACTGGATGAA
    GAACTGAGGG AGGCATCCGA AGCAGCTAAC ATTGACATGA AGCTGAAGGA GAAGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55607
    Clone ID Related Accession (Same CDS sequence) XM_006527152.2 , XM_006527152.3
    Accession Version XM_006527152.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527152.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
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    2941
    3001
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    3121
    3181
    3241
    3301
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    3421
    3481
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4621
    4681
    4741
    4801
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    4921
    4981
    5041
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    5281
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    5521
    5581
    5641
    5701
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    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGCATT GACATGAAGC TGAAGGAGAA GCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527215.1
    CDS586..9762
    Translation

    Target ORF information:

    RefSeq Version XM_006527152.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527152.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGCATT GACATGAAGC TGAAGGAGAA GCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55607
    Clone ID Related Accession (Same CDS sequence) XM_006527152.2 , XM_006527152.3
    Accession Version XM_006527152.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527152.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGCATT GACATGAAGC TGAAGGAGAA GCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527215.1
    CDS587..9763
    Translation

    Target ORF information:

    RefSeq Version XM_006527152.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527152.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA GTATGATGAA
    GAGAAATCTT TTAAGAGAAG TGTGAGCATT GACATGAAGC TGAAGGAGAA GCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55608
    Clone ID Related Accession (Same CDS sequence) XM_006527153.2 , XM_006527153.3
    Accession Version XM_006527153.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527153.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2641
    2701
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    3001
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    3181
    3241
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    4081
    4141
    4201
    4261
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    4381
    4441
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    4681
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    4981
    5041
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    5281
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    5521
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    5641
    5701
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    5881
    5941
    6001
    6061
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    6181
    6241
    6301
    6361
    6421
    6481
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    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
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    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA CATTGACATG
    AAGCTGAAGG AGAAGCCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527216.1
    CDS586..9726
    Translation

    Target ORF information:

    RefSeq Version XM_006527153.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527153.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA CATTGACATG
    AAGCTGAAGG AGAAGCCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55608
    Clone ID Related Accession (Same CDS sequence) XM_006527153.2 , XM_006527153.3
    Accession Version XM_006527153.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527153.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA CATTGACATG
    AAGCTGAAGG AGAAGCCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527216.1
    CDS587..9727
    Translation

    Target ORF information:

    RefSeq Version XM_006527153.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527153.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GACGGTCTGA ATGCCATCAT TGTGTTTGCT GCCTGCTTTC TGCCAGACAG CAGCCGGGCG
    GATTACCACT ATGTCATGGA AAACCTTTTC CTATATGTAA TAAGTACCTT AGAGCTGATG
    GTTGCTGAAG ACTACATGAT CGTGTACTTA AATGGTGCAA CCCCAAGGCG GAAGATGCCA
    GGACTAGGTT GGATGAAGAA ATGCTACCAG ATGATTGACA GACGGTTGCG CAAGAATTTG
    AAGTCATTCA TCATTGTTCA CCCATCTTGG TTCATCAGGA CAATCTTAGC AGTGACTCGG
    CCTTTTATAA GCTCTAAATT CAGCAGTAAA ATTAAATATG TGACTAGCTT ATCTGAGCTC
    AGTGGCCTGA TCCCCATGGA CTGCATCCAC ATTCCAGAGA GCATCATCAA CATTGACATG
    AAGCTGAAGG AGAAGCCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55609
    Clone ID Related Accession (Same CDS sequence) XM_006527154.3 , XM_006527154.2
    Accession Version XM_006527154.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527154.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
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    1321
    1381
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    1501
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    1801
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    1981
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    9001
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    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA ACATTGACAT GAAGCTGAAG
    GAGAAGCCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527217.1
    CDS587..9718
    Translation

    Target ORF information:

    RefSeq Version XM_006527154.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527154.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA ACATTGACAT GAAGCTGAAG
    GAGAAGCCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55609
    Clone ID Related Accession (Same CDS sequence) XM_006527154.3 , XM_006527154.2
    Accession Version XM_006527154.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527154.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA ACATTGACAT GAAGCTGAAG
    GAGAAGCCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527217.1
    CDS585..9716
    Translation

    Target ORF information:

    RefSeq Version XM_006527154.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527154.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA ACATTGACAT GAAGCTGAAG
    GAGAAGCCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55610
    Clone ID Related Accession (Same CDS sequence) XM_030250976.1
    Accession Version XM_030250976.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG ATATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030106836.1
    CDS590..9277
    Translation

    Target ORF information:

    RefSeq Version XM_030250976.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030250976.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG ATATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79979
    Clone ID Related Accession (Same CDS sequence) XM_017318226.2 , XM_017318226.1
    Accession Version XM_017318226.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318226.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGATCC CACGGCCAAC
    AAAAGCTCTG GTCAAGAGTC AGAGTCTATT CCAGAATATA CGGCTGAAGA GGAGCGAGAA
    GACAACCGGC TGTGGAGGAC GGTGGTCATC GGGGAGCAGG AGCAACGGAT TGACATGAAG
    GTCATTGAAC CCTACAGGAG AGTCATTTCT CACGGAGGAT ACTATGGGGA CGGTCTGAAT
    GCCATCATTG TGTTTGCTGC CTGCTTTCTG CCAGACAGCA GCCGGGCGGA TTACCACTAT
    GTCATGGAAA ACCTTTTCCT ATATGTAATA AGTACCTTAG AGCTGATGGT TGCTGAAGAC
    TACATGATCG TGTACTTAAA TGGTGCAACC CCAAGGCGGA AGATGCCAGG ACTAGGTTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGCGCA AGAATTTGAA GTCATTCATC
    ATTGTTCACC CATCTTGGTT CATCAGGACA ATCTTAGCAG TGACTCGGCC TTTTATAAGC
    TCTAAATTCA GCAGTAAAAT TAAATATGTG ACTAGCTTAT CTGAGCTCAG TGGCCTGATC
    CCCATGGACT GCATCCACAT TCCAGAGAGC ATCATCAAGT ATGATGAAGA GAAATCTTTT
    AAGAGAAGTG TGAGACTGGA TGAAGAACTG AGGGAGGCAT CCGAAGCAGC TAAAACTAGC
    TGCCTTTACA ATGATCCAGA AATGTCTTCT ATGGAAAAGG ACATTGACAT GAAGCTGAAG
    GAGAAGCCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173715.1
    CDS588..9839
    Translation

    Target ORF information:

    RefSeq Version XM_017318226.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318226.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGATCC CACGGCCAAC
    AAAAGCTCTG GTCAAGAGTC AGAGTCTATT CCAGAATATA CGGCTGAAGA GGAGCGAGAA
    GACAACCGGC TGTGGAGGAC GGTGGTCATC GGGGAGCAGG AGCAACGGAT TGACATGAAG
    GTCATTGAAC CCTACAGGAG AGTCATTTCT CACGGAGGAT ACTATGGGGA CGGTCTGAAT
    GCCATCATTG TGTTTGCTGC CTGCTTTCTG CCAGACAGCA GCCGGGCGGA TTACCACTAT
    GTCATGGAAA ACCTTTTCCT ATATGTAATA AGTACCTTAG AGCTGATGGT TGCTGAAGAC
    TACATGATCG TGTACTTAAA TGGTGCAACC CCAAGGCGGA AGATGCCAGG ACTAGGTTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGCGCA AGAATTTGAA GTCATTCATC
    ATTGTTCACC CATCTTGGTT CATCAGGACA ATCTTAGCAG TGACTCGGCC TTTTATAAGC
    TCTAAATTCA GCAGTAAAAT TAAATATGTG ACTAGCTTAT CTGAGCTCAG TGGCCTGATC
    CCCATGGACT GCATCCACAT TCCAGAGAGC ATCATCAAGT ATGATGAAGA GAAATCTTTT
    AAGAGAAGTG TGAGACTGGA TGAAGAACTG AGGGAGGCAT CCGAAGCAGC TAAAACTAGC
    TGCCTTTACA ATGATCCAGA AATGTCTTCT ATGGAAAAGG ACATTGACAT GAAGCTGAAG
    GAGAAGCCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79979
    Clone ID Related Accession (Same CDS sequence) XM_017318226.2 , XM_017318226.1
    Accession Version XM_017318226.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGATCC CACGGCCAAC
    AAAAGCTCTG GTCAAGAGTC AGAGTCTATT CCAGAATATA CGGCTGAAGA GGAGCGAGAA
    GACAACCGGC TGTGGAGGAC GGTGGTCATC GGGGAGCAGG AGCAACGGAT TGACATGAAG
    GTCATTGAAC CCTACAGGAG AGTCATTTCT CACGGAGGAT ACTATGGGGA CGGTCTGAAT
    GCCATCATTG TGTTTGCTGC CTGCTTTCTG CCAGACAGCA GCCGGGCGGA TTACCACTAT
    GTCATGGAAA ACCTTTTCCT ATATGTAATA AGTACCTTAG AGCTGATGGT TGCTGAAGAC
    TACATGATCG TGTACTTAAA TGGTGCAACC CCAAGGCGGA AGATGCCAGG ACTAGGTTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGCGCA AGAATTTGAA GTCATTCATC
    ATTGTTCACC CATCTTGGTT CATCAGGACA ATCTTAGCAG TGACTCGGCC TTTTATAAGC
    TCTAAATTCA GCAGTAAAAT TAAATATGTG ACTAGCTTAT CTGAGCTCAG TGGCCTGATC
    CCCATGGACT GCATCCACAT TCCAGAGAGC ATCATCAAGT ATGATGAAGA GAAATCTTTT
    AAGAGAAGTG TGAGACTGGA TGAAGAACTG AGGGAGGCAT CCGAAGCAGC TAAAACTAGC
    TGCCTTTACA ATGATCCAGA AATGTCTTCT ATGGAAAAGG ACATTGACAT GAAGCTGAAG
    GAGAAGCCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173715.1
    CDS586..9837
    Translation

    Target ORF information:

    RefSeq Version XM_017318226.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318226.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGATCC CACGGCCAAC
    AAAAGCTCTG GTCAAGAGTC AGAGTCTATT CCAGAATATA CGGCTGAAGA GGAGCGAGAA
    GACAACCGGC TGTGGAGGAC GGTGGTCATC GGGGAGCAGG AGCAACGGAT TGACATGAAG
    GTCATTGAAC CCTACAGGAG AGTCATTTCT CACGGAGGAT ACTATGGGGA CGGTCTGAAT
    GCCATCATTG TGTTTGCTGC CTGCTTTCTG CCAGACAGCA GCCGGGCGGA TTACCACTAT
    GTCATGGAAA ACCTTTTCCT ATATGTAATA AGTACCTTAG AGCTGATGGT TGCTGAAGAC
    TACATGATCG TGTACTTAAA TGGTGCAACC CCAAGGCGGA AGATGCCAGG ACTAGGTTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGCGCA AGAATTTGAA GTCATTCATC
    ATTGTTCACC CATCTTGGTT CATCAGGACA ATCTTAGCAG TGACTCGGCC TTTTATAAGC
    TCTAAATTCA GCAGTAAAAT TAAATATGTG ACTAGCTTAT CTGAGCTCAG TGGCCTGATC
    CCCATGGACT GCATCCACAT TCCAGAGAGC ATCATCAAGT ATGATGAAGA GAAATCTTTT
    AAGAGAAGTG TGAGACTGGA TGAAGAACTG AGGGAGGCAT CCGAAGCAGC TAAAACTAGC
    TGCCTTTACA ATGATCCAGA AATGTCTTCT ATGGAAAAGG ACATTGACAT GAAGCTGAAG
    GAGAAGCCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79980
    Clone ID Related Accession (Same CDS sequence) XM_017318227.1 , XM_017318227.2
    Accession Version XM_017318227.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
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    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA AACTGGATGA AGAACTGAGG
    GAGGCATCCG AAGCAGCTAA AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG
    GAAAAGGACA TTGACATGAA GCTGAAGGAG AAGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173716.1
    CDS585..9803
    Translation

    Target ORF information:

    RefSeq Version XM_017318227.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318227.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA AACTGGATGA AGAACTGAGG
    GAGGCATCCG AAGCAGCTAA AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG
    GAAAAGGACA TTGACATGAA GCTGAAGGAG AAGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79980
    Clone ID Related Accession (Same CDS sequence) XM_017318227.1 , XM_017318227.2
    Accession Version XM_017318227.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318227.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA AACTGGATGA AGAACTGAGG
    GAGGCATCCG AAGCAGCTAA AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG
    GAAAAGGACA TTGACATGAA GCTGAAGGAG AAGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173716.1
    CDS587..9805
    Translation

    Target ORF information:

    RefSeq Version XM_017318227.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318227.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA AACTGGATGA AGAACTGAGG
    GAGGCATCCG AAGCAGCTAA AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG
    GAAAAGGACA TTGACATGAA GCTGAAGGAG AAGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79981
    Clone ID Related Accession (Same CDS sequence) XM_017318228.2 , XM_017318228.1
    Accession Version XM_017318228.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318228.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA AGTATGATGA AGAGAAATCT
    TTTAAGAGAA GTGTGAGAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAA
    AAGGACATTG ACATGAAGCT GAAGGAGAAG CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173717.1
    CDS587..9802
    Translation

    Target ORF information:

    RefSeq Version XM_017318228.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318228.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA AGTATGATGA AGAGAAATCT
    TTTAAGAGAA GTGTGAGAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAA
    AAGGACATTG ACATGAAGCT GAAGGAGAAG CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79981
    Clone ID Related Accession (Same CDS sequence) XM_017318228.2 , XM_017318228.1
    Accession Version XM_017318228.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA AGTATGATGA AGAGAAATCT
    TTTAAGAGAA GTGTGAGAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAA
    AAGGACATTG ACATGAAGCT GAAGGAGAAG CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173717.1
    CDS585..9800
    Translation

    Target ORF information:

    RefSeq Version XM_017318228.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318228.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA AGTATGATGA AGAGAAATCT
    TTTAAGAGAA GTGTGAGAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAA
    AAGGACATTG ACATGAAGCT GAAGGAGAAG CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79982
    Clone ID Related Accession (Same CDS sequence) XM_017318229.1 , XM_017318229.2
    Accession Version XM_017318229.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGATCC CACGGCCAAC
    AAAAGCTCTG GTCAAGAGTC AGAGTCTATT CCAGAATATA CGGCTGAAGA GGAGCGAGAA
    GACAACCGGC TGTGGAGGAC GGTGGTCATC GGGGAGCAGG AGCAACGGAT TGACATGAAG
    GTCATTGAAC CCTACAGGAG AGTCATTTCT CACGGAGGAT ACTATGGGGA CGGTCTGAAT
    GCCATCATTG TGTTTGCTGC CTGCTTTCTG CCAGACAGCA GCCGGGCGGA TTACCACTAT
    GTCATGGAAA ACCTTTTCCT ATATGTAATA AGTACCTTAG AGCTGATGGT TGCTGAAGAC
    TACATGATCG TGTACTTAAA TGGTGCAACC CCAAGGCGGA AGATGCCAGG ACTAGGTTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGCGCA AGAATTTGAA GTCATTCATC
    ATTGTTCACC CATCTTGGTT CATCAGGACA ATCTTAGCAG TGACTCGGCC TTTTATAAGC
    TCTAAATTCA GCAGTAAAAT TAAATATGTG ACTAGCTTAT CTGAGCTCAG TGGCCTGATC
    CCCATGGACT GCATCCACAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAG
    GCATCCGAAG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAA
    AAGGACATTG ACATGAAGCT GAAGGAGAAG CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173718.1
    CDS586..9801
    Translation

    Target ORF information:

    RefSeq Version XM_017318229.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318229.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGATCC CACGGCCAAC
    AAAAGCTCTG GTCAAGAGTC AGAGTCTATT CCAGAATATA CGGCTGAAGA GGAGCGAGAA
    GACAACCGGC TGTGGAGGAC GGTGGTCATC GGGGAGCAGG AGCAACGGAT TGACATGAAG
    GTCATTGAAC CCTACAGGAG AGTCATTTCT CACGGAGGAT ACTATGGGGA CGGTCTGAAT
    GCCATCATTG TGTTTGCTGC CTGCTTTCTG CCAGACAGCA GCCGGGCGGA TTACCACTAT
    GTCATGGAAA ACCTTTTCCT ATATGTAATA AGTACCTTAG AGCTGATGGT TGCTGAAGAC
    TACATGATCG TGTACTTAAA TGGTGCAACC CCAAGGCGGA AGATGCCAGG ACTAGGTTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGCGCA AGAATTTGAA GTCATTCATC
    ATTGTTCACC CATCTTGGTT CATCAGGACA ATCTTAGCAG TGACTCGGCC TTTTATAAGC
    TCTAAATTCA GCAGTAAAAT TAAATATGTG ACTAGCTTAT CTGAGCTCAG TGGCCTGATC
    CCCATGGACT GCATCCACAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAG
    GCATCCGAAG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAA
    AAGGACATTG ACATGAAGCT GAAGGAGAAG CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79982
    Clone ID Related Accession (Same CDS sequence) XM_017318229.1 , XM_017318229.2
    Accession Version XM_017318229.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318229.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGATCC CACGGCCAAC
    AAAAGCTCTG GTCAAGAGTC AGAGTCTATT CCAGAATATA CGGCTGAAGA GGAGCGAGAA
    GACAACCGGC TGTGGAGGAC GGTGGTCATC GGGGAGCAGG AGCAACGGAT TGACATGAAG
    GTCATTGAAC CCTACAGGAG AGTCATTTCT CACGGAGGAT ACTATGGGGA CGGTCTGAAT
    GCCATCATTG TGTTTGCTGC CTGCTTTCTG CCAGACAGCA GCCGGGCGGA TTACCACTAT
    GTCATGGAAA ACCTTTTCCT ATATGTAATA AGTACCTTAG AGCTGATGGT TGCTGAAGAC
    TACATGATCG TGTACTTAAA TGGTGCAACC CCAAGGCGGA AGATGCCAGG ACTAGGTTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGCGCA AGAATTTGAA GTCATTCATC
    ATTGTTCACC CATCTTGGTT CATCAGGACA ATCTTAGCAG TGACTCGGCC TTTTATAAGC
    TCTAAATTCA GCAGTAAAAT TAAATATGTG ACTAGCTTAT CTGAGCTCAG TGGCCTGATC
    CCCATGGACT GCATCCACAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAG
    GCATCCGAAG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAA
    AAGGACATTG ACATGAAGCT GAAGGAGAAG CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173718.1
    CDS588..9803
    Translation

    Target ORF information:

    RefSeq Version XM_017318229.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318229.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGATCC CACGGCCAAC
    AAAAGCTCTG GTCAAGAGTC AGAGTCTATT CCAGAATATA CGGCTGAAGA GGAGCGAGAA
    GACAACCGGC TGTGGAGGAC GGTGGTCATC GGGGAGCAGG AGCAACGGAT TGACATGAAG
    GTCATTGAAC CCTACAGGAG AGTCATTTCT CACGGAGGAT ACTATGGGGA CGGTCTGAAT
    GCCATCATTG TGTTTGCTGC CTGCTTTCTG CCAGACAGCA GCCGGGCGGA TTACCACTAT
    GTCATGGAAA ACCTTTTCCT ATATGTAATA AGTACCTTAG AGCTGATGGT TGCTGAAGAC
    TACATGATCG TGTACTTAAA TGGTGCAACC CCAAGGCGGA AGATGCCAGG ACTAGGTTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGCGCA AGAATTTGAA GTCATTCATC
    ATTGTTCACC CATCTTGGTT CATCAGGACA ATCTTAGCAG TGACTCGGCC TTTTATAAGC
    TCTAAATTCA GCAGTAAAAT TAAATATGTG ACTAGCTTAT CTGAGCTCAG TGGCCTGATC
    CCCATGGACT GCATCCACAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAG
    GCATCCGAAG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAA
    AAGGACATTG ACATGAAGCT GAAGGAGAAG CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79983
    Clone ID Related Accession (Same CDS sequence) XM_017318230.1 , XM_017318230.2
    Accession Version XM_017318230.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9168bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA AGTATGATGA AGAGAAATCT
    TTTAAGAGAA GTGTGAGCAT TGACATGAAG CTGAAGGAGA AGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173719.1
    CDS585..9752
    Translation

    Target ORF information:

    RefSeq Version XM_017318230.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Drosophila) (Prune2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318230.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA AGTATGATGA AGAGAAATCT
    TTTAAGAGAA GTGTGAGCAT TGACATGAAG CTGAAGGAGA AGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79983
    Clone ID Related Accession (Same CDS sequence) XM_017318230.1 , XM_017318230.2
    Accession Version XM_017318230.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9168bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein prune homolog 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318230.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA AGTATGATGA AGAGAAATCT
    TTTAAGAGAA GTGTGAGCAT TGACATGAAG CTGAAGGAGA AGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173719.1
    CDS587..9754
    Translation

    Target ORF information:

    RefSeq Version XM_017318230.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus prune homolog 2 (Prune2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318230.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGGATC GAAGTAAACA ATTGGAACAG 
    GTCCACGCAG TTATTGGACC TAAGTCATGT GACCTGGATT CTCTCATTTC TGCCTTCACA
    TATGCATACT TTCTGGACAA GGTCAGTCCT CCCGGGGTCC TCTGCTTGCC AGTGCTGAAT
    ATCCCAAGAA CTGAATTCAA CTACTTCACA GAGACAAGGT TTATTTTAGA AGAGCTGAAC
    ATCCCTGAAT CATTCCACAT ATTCCGAGAT GAGATTAACC TGCATCAGCT GAACGATGAA
    GGGAAACTGT CGATAACGCT CGTGGGCAGC CACGTCCTGG GGAGTGAGGA CAGAACTTTG
    GAATCGGCAG TTGTCAGAGT CATTAACCCC GGAGAGCAGA GTGATGGTGA GCTGGGGTTC
    CCGGAGACTT CCTCCTCTCT GGTGCTGAAG GAGCTCCTGC GTGAGGCTCC AGAACTCATC
    ACCCAGCAGC TGGCACATCT CCTCAGAGGC AGCATCCTGT TCACGTGGAT GAGTATGGAT
    CCTGAGCTCC CGGAGAAGCA GGAGGAAATC CTCTCCATCC TGGAAGAACA GTTTCCCAAC
    CTCCCTCCGA GGGATGATAT CATCAACGTG CTGCAAGAGA GTCAGCTCAG TGCTCAGGGT
    TTAAGTCTTG AACAAACAAT GCTGAAAGAT CTAAAGGAGC TGTCAGACGG TGAAATAAAA
    GTGGCCATCA GCACCGTGAA CATGACCCTG GAGGACTACC TGCTTCACGG CAACATCACC
    AGTGATTTAA AAGCCTTCAC AGACAAGTTC GGCTTTGACG TCCTCATTCT GATCTCAAGC
    TTCACATGGG AGGAACAGCA GAGGCAGCAG ATCGCCGTGT ACTCCCAGAA CCTGGAACTG
    TGCAGCCAGA TTTGCTGTGA GCTAGAAGAA AGTCAGAACC CTTGCCTGGA ACTGGAGCCC
    TTCGAATGTG GCTGTGATGA GATCCTGGTA TACCAGCAGG AAGACCCTTC TGTGACCTCC
    GACCAGGTGT TTCTTCTTCT TAAGGAAGTC ATCAATAGGA GGTGTGCAGA GATGGTCTCT
    AACAGTCGGA CATCCTCAAC AGAAGCCGTG GCAGGCAGCG CCCCGCTGTC CCAGGGCTCT
    TCCGGGATTA TGGAATTGTA TGGATCTGAC ATAGAGCCAC AGCCCAGCTC TGTGAATTTC
    ATAGAAAACC CCCCAGAGCT CAACGATTCT AACCAAGCTC AGGCGGATGG CAACATAGAC
    CTGGTTAGCC CTGACAGTGG GTTGGCCACC ATCAGAAGCA GTCGCTCGTC CAAGGAAAGC
    TCCGTCTTCC TCAGTGACGA CAGCCCCGTG GGAGACGGTG GTGCGCCTCA CCATAGCCTT
    CTCCCAGGAT TTGACTCCTA CAGCCCCATT CCTGAAGGGA TAGTTGCGGA GGAGCATGCT
    CACTCCGGGG AACACAGTGA ACACTTTGAC CTCTTCAACT TTGATTCAGC GCCCATAGCT
    TCAGAGCAGT CCCAGCCGTC TTCCCACTCT GCAGACTACT CGCCAGAAGA CGACTTCCCC
    AACAGCGATT CATCAGAAGG GAACCTCTCT GCCGGGCCTA AGGGGCTTGG TGAGATGGGG
    ATCAACATGT CCAATTACTC CTCTAGTTCT CTTTTGTCAG AGGCTGGTAA AGACAGCCTT
    GTGGAATTTG ATGAAGAGTT TATCCAGCGA CAAGAAAGCT CAGGGGATAA CTCTGAAAGA
    AATTTAAGCC TGACATGTTT TGCGGGAGAG GAGCCTTCTT CCCCTGAAAG GCTGAAGAAT
    CCTGGAAAGA TGATCCCACC AACACCTATG AACAGCTTTG TGGAAATCTC ACCATCAAAT
    GAGGAGCCAA CTCCACTCTA TCCTGAAGAT ATAATCCAAA ATGCAATTGA CACCGGACAT
    CTGGGCCCAC CTCAGGCCCG TGCACGGTGC AGAAGCTGGT GGGGTGGCTT GGAGATTGAC
    TCTAAAAACG TTGTAGATAC ATGGAACGCC AGTGAACAAG AATCTGTCTT CCAGAGCCCT
    GAACCATGGA AAGATCCTAA GCCTGAGCCA GTTGAGAGGA GAACCTCAGA TTCCACATTC
    CAACCAAAGA GTCTTGAATT CTCAAAGTCA GATCCTTGGG AATCTGAGTT TGGACAGCCT
    GAACTGGGCA ACAAAGAAGC CCAGGACCAG AAGGAGGAAA GCTTGCAATA CCAGCACTTG
    CCTACAGTAA GGCCACATTT GACAGATGCC TCTCCTCATG GGACAAACCA CTTGATAGAG
    GACTTTGCTG CTTTGTGGCA TTCAGGACAC TCACCAACAA CAATGCCTGA GCCCTGGGGA
    AACCCCACCG ATGCTGGTGA AGCTGCAGTT ACGATGTCCT TCCCCACCTG GGGTGCATTT
    GATAAAGAAG AAGATAATGC TGACACATTA AAGAATACTT GGAATCTCCA TCCCACTAAC
    AACGAGACAC CTTCAGGGCA GGAACCAAGT GAATGGGCTA TGGGCCAAAG TGGGTTTTCA
    TTCCCTGCAG CAGACCTACT TGACAATCCC CTCATTGAGG TAAATAAAGA TGCAGCTCCA
    GAAATCTGGG GCAAGAACAA CAGCTCCAAG GATACCAGTC TTACGTCTGG AAGTCCCACT
    TCAGATCTGG GTCAAACGTG GAATAATTCT AAGCTACCAG GGGAAGACCA GAATGGCTTG
    GTGGATCCTA AAGCTACAGG GAAGGTATAT GAGAAGGAAG GGTCCTGGAG CCTCTTTGAG
    GAGAGTGCTA AGAAAAGAGG GGCTGATGTT TTAGCTCCTT GGGAAGATTC CTTCTTGTCC
    TATAAATGTT CTGATTACAG TGCATCCAAC ATAGGAGAAG ATTCAGTCCC ATCTCCATTA
    GACACCAACT ACTCCACCTC TGACTCTTAC ACATCACCAA CATATGCTGG GGATGAAAAA
    GAAATAGCAA ACAAGCCAGT TGACAAAGAT AATGGTTTTG AGGCAAAAGA TGCTGAATTT
    CCTGCAGAGG GGCTTGAGGT TCTTGCAACA TCATCACAGC AGTCTCAGAG AAATCGAATT
    GGTTCTGGTC CTGGGAATCT AGACATGTGG GCATTGCCTC ACACAGAAGA TAAGCCTGAA
    GGAAATGACG CACATCACCC AGATAGCGAT GCGCTCAAGA CGGAGCACGC AGAAGACAAA
    AATGCTTCCA TGGAGGATGA CGTGAGGGAA AGCAGCCCGT CCAGTTACGA TGACCCTAGC
    ATGATGCGAC TGTACGAGGC AAACCGGCAG CTCACCCTTC TACACAGCAA CACCAACTCA
    CGCCAGGCAG CCCCTGACAG CCTTGACACA TGGAACCGAG TGACTCTGGA GGACACGCAG
    TCCACTGCAA CGATCTCGGA CATGGATAAT GACTTGGATT GGGATGACTG CAGTGGGGGC
    GTGGCGATCA GTGGGGATGG TCAAGCAGAA GGTTACATAG CTGCAAACAG TGAACCTGAA
    ACCCGGTTCT CAGTGAAGCA GCTGGAACCA TGGGGTACAG AACATCAGGA AGCAAATCAG
    GTAGACTGGG ATCTCTCTGC CTCTGCTGAG CCCACAGGGG ATCCTGGTCC CAGTGAATAC
    CAGACCCTAA ATGAGAAAAC TGGGCAGTTA ATTGCAAACA GTATTTGGGA TTCTGTCATG
    GGAGATAAAA ATATGCCATC ATTCAGATTA CCTAGCCCAC CAAATACTGT AGATATGGAA
    CACGGCACTT GGCCTTCTGA AAGTCCCAGG CACTCAAATG GTAAGGACAG CCACATGTTA
    GAAGCCTCTA GACTCAGTGA GTCGGGAGGG CTTACGTCTC AACCTGTCAA CCAGGACACG
    TGGGGAGACT CCCAAGGTGA CACAGCATCC TCGGTGACCG GACTGGCAAG TCCAGAACAC
    TTTGCACAGT CAGATCCATG GACAGGCCAT ACTTATGGGC AGAGTGAAAG TGAGATTGAA
    GGCCTGGTGG CCTCTGATTG TGAGCATCTT GACAAGGAGG CAGCGCTAGG CTCTGGAGTG
    AATGGTGCCC CGTGGGCTTT TGGAAAAAAG CCCAGGGACC AGGAATTCTC TTCTTCAGAT
    GCATTTGAAC ACCAAGACAT CTCCAGTGCA TCAGGCAAAA TCAGCTCTCT TTCAGTCACC
    TCCAGTCCTC AGAGTGAGGA ACCAGGGGAG GCCTTAGAAG TCGAGAAGGA GCCTTTCATT
    CTAGACTCCC TTGCTGTGCA AACAGAGACA TTCACATGGG ATTTGCAAAG CAAAGACACC
    CATGAAGAAT CTCTAGTGGA TCATAGAAAT TTAGGTGAAG CCAACACAAC ATTGGATAGG
    ATTAATCCAA TGAAAAACAG GCCCTTATCT GGAATGGAAC TTGAGAAAAC TGAAGCCTGT
    ACCATTCTGA AGCCAGAAAG AGCAAATGGA AAACTACTTT ATGAATCTTC TCAAGACTTT
    GGTGTTTGGG ATGGCCCGAT GGATAGTGAT GTGTGGGATA GTCATATAAG TTATGAGACA
    GCTATGAATT CTACAGGTCA GAGAACTGAG GAAAGATCTC TAGAAGCCCT TTCACCAGGA
    AACTATGACA GAGACAGCCT CTCTAGTGGG TGTACGCATT CCAGTGCATC AAGTCCTGAC
    CTACATGATT CCTCAGTAGC CTTGTCCTCC TGGACTTACG GACCCAGTGC TGAACATCAG
    AAAGAGAATC ATGATGATGC GAACAAACAG ATTCACCAAG AATCAGAACT ATTTACCACC
    GAAGCCCATG TAGATGTCAT TACTGAAATG AAGGACTTTG TAGAAAACAG GGAGGATGGC
    TTTGGAAAGA TGTCCAATCA GGAAGATCCT CAATTCCCAG AGATTCCAAA TGATCCCTTC
    AACAGTGGTC CCTCATCCTC TTCATCCAGC CTTGGAGCAG ATAAATATTC AGAATATTCT
    CATGCATATC AGGAAGGGGA TCTAACGACT AAACGTCAAG AGGAAAATGA ACTTGGTTTT
    CTGGAAATTG TGGAGCCAGA GGGTACCAGG ATCATATCCA CAAGCTCAGG TTCTGGCAAT
    GACAGTGGGG GTGACGAAGA GTTGCTAGAG AAGGAGCTCC ATTTGGCCAC AGTTGCTCAG
    AGTGAAGCTG GGGCTTGCAC TTCATTGCAT GAACCTGCGT TCTCGGCCAC AGAGCATTCC
    AAGCCTGAGT TTTCTGTCTT TGTGGGTAGT TTGGAGTCAA TAGAGAAAGA GAACAAGTCA
    AGCCCGTTCA GTGACAGTCA ACAGAGCAGC CCTGGTCAGT GGATTTTGTC ACCACTCATG
    CAGGCTGACA CACAGGACAC ATGTAAGGAG GAGACCAGAG CTGCAGAAAC GGGGACAATG
    GATACCACGT GGCATGGATC TGCCAGCACG GAAGCTAAGA ATGGGGACCC TGACAAATTG
    GAAATGCTTG GCTTCTCAGC TGACAGCACT GAGTGGTGGA ACGCTGGGGC ACAGGAAGGG
    AGAGCAAACG CCGGTATGTC TGCAGAGGAG TTGTCTAACT CAGAGGGTGA GCTAGAGCCT
    ACTTCTCCAG TGTTCCAGAA CGCTGGTCCG TGGAGCTTAC CCATTCAGAA TGATAGTGAA
    CCTGTGGACA CAGGCAGCAC CAATCCTTTC CGTGGTAAAC TAAAGTCACC CGTCCTAGAT
    AGTCATGGAG ACAAGTCACA GGAGAAACTT TGGAACATTC AACCTAAGCA GCTGGATTCA
    GATGCCAATC AACTCAGCCA GCTTGTGATC CTGGACCAAA TGAAGGACAA AGATTCTGGA
    CAGCAAACAG CCATGTCTCC TGCTGCTGGT GATCTTCCTG CAGAAACTCT CACCCAAGGG
    CAGGGCCGGG AATCTATGCT GTCTGTCTGG GACCGTGCAG AACCAGCATT AACTCACAGA
    GATGAAAATG GATGTGTTAG CACTGGGGTT TCACCCACTG AGTGCCAACA AGAGAACCAG
    TGGGAACCAG AAAAACCCTA TCTCAGCCAC GTGACACATT CAAGCACTCC CACAGAAAAT
    GCCCTCGAGA GCAATGCCCC CACACAACTG ATGAGGAAGC TGGACTCTGA TTGGAATAGC
    CCCAGCCCCA GCGAACCCCA ACATAACTTT GTTCCAGATA TTTTACATGG CAACTTCGAA
    GAGGGTGGGC AGCTGGCCTC GGCGTCACCT GATTTGTGGA TGGATGTCAA GCAACCCTTC
    AGTTTTAAAG TAGATAGCGA GAATCCTGAT ATATTGACTC ACTGTGACCA TGACAGCAAC
    TCTCAGGCAT CCAGCAGCCC TGACGTGTGC CACGATTCTG AAGGGGAGCA AAAGATGGAG
    AAGCATACTG CTGTTTACCT GGGACTTGAA GTGGAACCCA GTGAGTTTTC TCTCACCGAG
    CCAAACATGA ATGATGAACC AACTTGGGAA CCTGAGCAGG AAAGTCTCCC ACACAATTCA
    GAACTCCATT CCGAACACGC AATGCCCTTG CCTCCAATCG ACAGTCAGAA TGATATAAAT
    AACTCATCTA AGCCTGCCTC TTCAAGAAGT TCCCCAGAAC CCTCTGATAT GCGCGGAGAC
    AACAACACAA GTGTAACATC GATGGAAGAA GACACCAACC CAGAGGTAGA AGCAGTGGAC
    AGCGTGACGA TCCCAGGTCA CTTCCCCAGA AGTGAAGACG CTGACACTTT TGAAGCTCAC
    CAAGAGGTCA GTGTGGAAGT CGACGACTCT TGGGTGTCAA AAGACCTTTG TCCTGAAAGT
    CAGACAGGCA CTAGAGCTCT GCTTGACTGT GAGCAACCGT TTGCTTCCGA GAGCCCTGCT
    GTACTTACGG ACATCTTCCT AACCTCAGAC ACCTGCCTGG ATGTAAGCGA AGCTGCCTTG
    GATCACAGTT TCAGTGATGC CTCCGGCCTC AACACTTCCA CAGGCACTAT AGACGACATG
    AGTAAGCTGA CTCTATCGGA AGGCCATCCC GAAACACCAG TTGACGGGGA TGCAGGGAAG
    CAAGATGTCT GTTCATCTGA AGCTTCCTGG GGTGATTTTG AATATGATGC AATGGGTCAG
    AATATTGACG AAGAGCTGAT GAGAGAGCCT GAACACTTCC TCTATGGTGG TGATCTTCCG
    TCAGAGGAAA GCGCGCTGAA GCAATCACTG ACACCATACA CACCCCCCTT TGATTTGTCC
    TATCTCACGG AACCTACTGG ATGCACTGAA ACTGCACAGG GAGCTGAGTC TCCAGGTGAT
    GAATCTCTGG GAAGTGACGC GGCAGAGATG TTGCTTTCTG CTCTTCCAGA TCACAGGGAG
    GAAGACAAGG CTGAGACCAA CATCAGGAAA CCTAGATACC AGATGACTGT GTTGCATATT
    CATGAAGACC CTGAGGCTCT TTCATCACCT GTGGGAGGAA CAGGTTCCAA TAATGAGTCA
    TCTCCATCAA ACATTGACTG GGAAATAGAA ACAGATAATT CAGATTCACC AGCAGGTGGC
    GACATGAAAC CACCAAACGG CAAGGAGATA CTGGAGTTAG AAGAAGATGA GAAAGTAATT
    CCCACGAAAG GGCCAAAGCA AACAGAATTA GAATACAAAG AAGAAAAACA GCCAGAGCAA
    AGTGAAGACC ATCAGGTACT AGCTGTGGAC TACATCCTTG TAAGCCATGA AAAAGATTCA
    CCATTGAAAC CAGAGGCCAG GGAAGCAAGA GAAAACATAC CCGAATTGGA ACAATTATCC
    ATAGGTTCCA GAGAAACAGG GCTACCAGAG ACTCAGTTAT CAGGCACCCC AGACACATGT
    CAGTCTGAGT TTTTAAATGA TGTCAAAGTT CATTCTGCAG AGAGAATGTC TTCGTCTTCC
    AACCACGAGA GTGCATCTCT TGAAAACCCT GCACAAGACC AGAGTTGGAT GGTCTTGAGC
    CATAGTGAGG TTGGTGACCC GCCTACAGAA ACCAGGGACT CGGGGCCTGA GTCTCCTGGT
    AGAACTCCGG AGCCGTTCTT GAGTCTCAGT TTGGACAAGG GCCCCAAGTC CCAGGTTCTG
    GAAAGAAATA AGCCTCTAAA TTCTTTAGCA CTAGAGGAAG TTGCTGGTTT GAGCAGCCAA
    TCAAGGAACA TCGAGAGACA AGGTCAAGCT GGTCTAGATG CAGTTCCAAC CCAGGCGGCC
    ACCCACGACA ATGAATGGGA AATGCTTTCG CCACAGCTCT CCCGGAAAAA CAGGAACCCT
    CCACAGGAAA TGGAAGAAGA GACACAGTGC CCTGAGCCTG GACCCAGAAA GCCAAGACTG
    AAAGGACCCC CGTCAGAGGA TGAAGGAATG GATATCCACT TCGAGGAGGG AGTGCTGAGT
    CCCAGTGCCG CAGACATGAG GCCCGAACCT CCCAATTCTC TGGATCTCAA TGGCAGTCAC
    CCTAGAAGAA TCAAGCTCAC AGCCCCAAAT ATCAACCTCT CTCTGGACCA AAGTGAAGGA
    TCTATTCTCT CTGATGATAA CCTAGACAGT CCGGATGAGA TTGACATCAA TGTGGATGAA
    CTCGATACCC CTGATGAAGC AGACTCTTTT GAATATACTA ACCATGAAGA TCCCACGGCC
    AACAAAAGCT CTGGTCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCTGA AGAGGAGCGA
    GAAGACAACC GGCTGTGGAG GACGGTGGTC ATCGGGGAGC AGGAGCAACG GATTGACATG
    AAGGTCATTG AACCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTG
    AATGCCATCA TTGTGTTTGC TGCCTGCTTT CTGCCAGACA GCAGCCGGGC GGATTACCAC
    TATGTCATGG AAAACCTTTT CCTATATGTA ATAAGTACCT TAGAGCTGAT GGTTGCTGAA
    GACTACATGA TCGTGTACTT AAATGGTGCA ACCCCAAGGC GGAAGATGCC AGGACTAGGT
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGC GCAAGAATTT GAAGTCATTC
    ATCATTGTTC ACCCATCTTG GTTCATCAGG ACAATCTTAG CAGTGACTCG GCCTTTTATA
    AGCTCTAAAT TCAGCAGTAA AATTAAATAT GTGACTAGCT TATCTGAGCT CAGTGGCCTG
    ATCCCCATGG ACTGCATCCA CATTCCAGAG AGCATCATCA AGTATGATGA AGAGAAATCT
    TTTAAGAGAA GTGTGAGCAT TGACATGAAG CTGAAGGAGA AGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.