Plekhm1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plekhm1
Entrez Gene ID	353047
Full Name	pleckstrin homology domain containing, family M (with RUN domain) member 1
Synonyms	AP162,B2,BC038943,D330036J23Rik
General protein information	Preferred Names pleckstrin homology domain containing, family M (with RUN domain) member 1 Names pleckstrin homology domain-containing family M member 1 PH domain-containing family M member 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_183034.1	NP_898855.1	pleckstrin homology domain-containing family M member 1
NM_183034.2	NP_898855.1	pleckstrin homology domain-containing family M member 1

Pathways from BioSystems

Homologies

	PLEKHM1	XP_002800605.1
Gallus gallus (chicken)	PLEKHM1	NP_001026095.1
Danio rerio (zebrafish)	plekhm1	NP_001082872.1
Pan troglodytes (chimpanzee)	PLEKHM1	XP_001140671.1
Mus musculus (house mouse)	Plekhm1	NP_898855.1
Bos taurus (cattle)	PLEKHM1	XP_002696128.2
Rattus norvegicus (Norway rat)	Plekhm1	NP_001009677.1
Homo sapiens (human)	PLEKHM1	NP_055613.1
Xenopus tropicalis (tropical clawed frog)	plekhm1	XP_002933223.1

Gene Ontology
Bibliography

Related articles in PubMed

PLEKHM1/DEF8/RAB7 complex regulates lysosome positioning and bone homeostasis.
Fujiwara T, Ye S, Castro-Gomes T, Winchell CG, Andrews NW, Voth DE, Varughese KI, Mackintosh SG, Feng Y, Pavlos N, Nakamura T, Manolagas SC, Zhao H
JCI insight1(17)e86330(2016 Oct)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

PLEKHM1: Adapting to life at the lysosome.
McEwan DG, Dikic I
Autophagy11(4)720-2(2015 Apr)

TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates Osteoclast Acidification and Resorption.
Witwicka H, Jia H, Kutikov A, Reyes-Gutierrez P, Li X, Odgren PR
PloS one10(5)e0127537(2015)

High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes.
Brommage R, Liu J, Hansen GM, Kirkpatrick LL, Potter DG, Sands AT, Zambrowicz B, Powell DR, Vogel P
Bone research214034(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identified PLEKHM1 as an endolysosomal adaptor platform that acts as a central hub to integrate endocytic and autophagic pathways at the lysosome
Title: PLEKHM1: Adapting to life at the lysosome.

TRAFD1, in association with Plekhm1/Rab7-positive late endosomes-early lysosomes, has a previously unknown role in vesicle trafficking, acidification, and resorption in osteoclasts
Title: TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates Osteoclast Acidification and Resorption.

results indicate that LIS1 is a previously unrecognized regulator of osteoclast formation, microtubule organization, and lysosomal secretion by virtue of its ability to modulate dynein function and Plekhm1
Title: LIS1 regulates osteoclast formation and function through its interactions with dynein/dynactin and Plekhm1.

The following Plekhm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plekhm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08322	NM_183034.2 Latest version!	Mus musculus pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OMu08322	NM_183034.1	Mus musculus pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plekhm1 ( NM_183034.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_898855.1 Mus musculus pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), mRNA.

Price & Availability↑

CloneID	OMu08322
Clone ID Related Accession (Same CDS sequence)	NM_183034.1 , NM_183034.2
Accession Version	NM_183034.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-25
Organism	Mus musculus(house mouse)
Product	pleckstrin homology domain-containing family M member 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC053079.1. ##Evidence-Data-START## Transcript exon combination :: BC053079.1, AK155357.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGCTCTCAG TGGAGAATGG CCTGGACCCT CGTGCTGCCA TCCAGGTCAT CAAGAAGAAG 
CTCGTGGGAT CCGTGAAGGC TCTGCAGAAA CAGCATGTGT CTCTGGACAC AGTGGTCACC 
AGTGAAGATG GAGATGCCAA CACCATGTGC AGTGCCCTGG AGGCTGTGTT CATCCACGGC 
CTGCATGCCA AGCACATCCG CGCCGAGGCT GGCGGCAAAA GGAAGAAGCA CACTCACCAG 
AAGGCTCTGC CTCAGCCTGT CTTCTGGCCC CTTCTGAAAG CCATCACCCA CAGACACATT 
GTCTCAGATT TGGAGCACCT GGTCTTCATC AACACAGATG TAGGCCGTTG CCGAGCCTGG 
CTGCGGTTGG CCCTCAATGA CGGTCTGATG GAGTGCTATC TGAAACTGCT CCTGCAGGAA 
CCTGCACGGT TGTGCGAGTA CTATCAGCCC ACAGCCCTGC TTCGAGATGC CGAGGAGGCT 
GAGTTCCTCC TGAGCTTCCT GCAAGGACTA ACATCCTTGT CCTTCGAACT CTCCTACAAG 
TCAGCCATCT TAAATGAATG GACACTCACC CCATTGTCGC TCTCTGGGCT TTGCCCCCTC 
TCTGAACTCG ACCCTCTCAC TACCTCTGGT GCAGAACTAC AGCGGAAAGA GTCTCTGGAT 
TCAATTTCCC ATTCCTCGGG CTCTGAGGAC ATCGAAGTCC AACACTCAGG CCATAAGATC 
CGCAGGAACA GGAAGCTCAC TGCCTCTTCC CTCAGCCTGG ACACAGCCAG TTCATCCCAG 
CTGTCCTGCA GCCTAAATTC TGACAGCTGC CTGCTCCAAG AGAATGGTCC CAAGAGTCCA 
GACCATTCTG AGGAACCCAT GTCCTATGAC TCAGATCTGG GCATGGCAAA CACCGATGAC 
CCCGACAGGT CTCTGCAAGA GGTGTTGTCG GAATTCAGCA AAGCCCAGGT AAATTCTGCA 
CCAAGCAGCG GACCAAACCA AGAGCCAGAC ACCCCCATGT TCCAGACGCC CCTCTCCCTC 
CATAGCCTAG CTACCAGCAC ACACCTGCAC TTTGAGGGAT CTGAAGAGCT CTTTCCTGCC 
CACAAATCCT CTGGGACATC AAGTGGTGGC CATAAGCATC AGCTACTGCC CCAGGAGACC 
CCAGACGAGA AGCAGCTAGG CACTGCCCAA GCTGGCCCTG CACAGAGCAC TTCAGACCAG 
CAGCCTTCTA GTCCTGTGGG TGGAGCAGCT GGCCAAGGGA GTGGCCCTTG GAAGGCCCTG 
GAGTATGGCA GAGTCGGACC GAAGCTTGTG GTTTCCTCTC CTACGAGTCC GAAAGGCAAG 
AGCTGGATTT CTGAAGATGA CTTCTGCCGA CCTCCCCAGG AGCCTGCTCT AAAGAGCGCT 
GCAGGTCTCT GCACATCTCC TGTGCAAGAC ACTCCAGAGT CAAGGGCTGC TCTCCACGGG 
CCTTTTTCTC AAGGACCAAG AAAGAGCTGT TCCTTGGGGG CATTAGACAA AGCATGTGTG 
CCCTCACAGG CCTGTGGAAA TGCCCAGCCA GCTCCAGCCC CAGCTCCAGC TCCAGCCCCA 
GCCCCAGCCC CAGCTCCAGG GGTGACACAA GACCATAAAA ACTTCTGTGT GGTGCACCGA 
AGGCAAATGG GGCTGTCCAA CCCATTCCGG GGCCTCATGA AGCTGGGCAC AGTGGCCCGG 
CGAGGAGCCA TGGGCATCTG GAAGGAATTC TTTTGTGAGC TGTCCCCGTT GGAGTTCCGC 
CTCTACTTGA GTGATGAGGA ACGCACCTGT GTGGAGAGCT GCTCCCTGCT CCGATGTGAG 
GCTGTGGGGC CAGCACACAG TGATGGACGT TTTGAGTTGG TCTTCTCGGG CAAGAAGCTG 
GCTCTCCGTG CCTCCTCCCA AGACGAGGCT GAGGACTGGC TTGACCGCGT ACGGGAGGCT 
CTGCAGAAGG TGCGCCCTCA GCAGGAAGAT GAGTGGGTGA ATATCCAGTA CCCAGACCAG 
GCTGAGGATG CCCCAGAGGC TCCACCAGAC AGCCTCCCCC CCTATTCAAC TCTACTCCCC 
GAGCCTGCGG GTGCCCAGGG CATGCAGTTG GACTGGACAT CTGCCCAGGT TCCAGAACCA 
GATGCTATCA AAGAGTCCCT TTTGTATCTG TATGCAGACC GGACCTGGGT CCCCTATATC 
TTTTCCCTGT CCTTGGAGTC TCTGAAATGT TTCCGAGTGA GAAACAATGA GAAGATGCTA 
AGTGATAGCC ATGGAGTGGA GACCATCCGA GACATCCTGC CAGACACCAG CCTTGGGGGC 
CCAGCCTTCT TCAAAATCAT CACGGCCAAG GCCGTCCTGA AACTGCAGGC CAAAAACACC 
GAGGAGGCCA CCCACTGGAG AGACCTGGTC CGGAAAGTGC TGGCATCTTA CCTAGAGTCA 
GCTGAGGAGG CTGTGACACT TGGTGGGAGT CTGGATGAAA AATGTCAGGA GGTGCTGAAG 
TTTGCTACCA GGGAGAACGG CTTCCTGCTA CAGTACCTTG TGGCCATCCC GACCGAGAAG 
GGCCTCGACT CCCAGGGCTG CTTCTGCGCA GGCTGCTCCC GACAGATCGG CTTCTCCTTT 
GTACGACCCA AGCTCTGTGC CTTCTCTGGC CTGTATTACT GTGACTTCTG CCACCAAGAC 
GATGCCTCAG TGATTCCAGC CAGAATCATC CACAACTGGG ACCTCACGAA GCGCCCGGTA 
TGCCGGCAGG CCCTGAAGTT CCTGGCCCAG ATCCGGGCCC AACCTCTCAT CAACCTGCAG 
CTGGTGAACG CCTCTCTGTA TGAGCATGTG GAACGCATGC ACCTCATTGG CAGGAGCCGG 
GAGCAGCTGA AACTTCTGGG GGACTACCTG GGCCTGTGCC GAAGTGGTGC TCTGAAGGAG 
TTGTGCAAAA GGCTGAGCCA CAGGAATTAT CTCCTGGAGT CTCCACACAG ATTCAGTGTT 
GCTGACCTCC AGCAGATTGC AGAGGGGGTA TATGAAGGGT TCCTCAAAGC CCTGATTGAA 
TTCGCATCCC AGCATGTCTA CCACTGTGAC CTGTGCACCC AGCGAGGCTT CATCTGCCAG 
ATCTGCCACC ACCAGGACAT CATCTTTCCC TTTGAGTTTG ACACCACAGT CAGGTGTGCT 
GAATGCAGAA CTGTCTTCCA TCAGAGCTGC CAGGCTGTGG TGAGGAAGGG CTGTCCCCGA 
TGTGCCCGCC GGCGCAAGTA CCAGGAACAG AACGTTGTCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_898855.1
CDS	114..3338
Translation	MLSVENGLDPRAAIQVIKKKLVGSVKALQKQHVSLDTVVTSEDGDANTMCSALEAVFIHGLHAKHIRAEAGGKRKKHTHQKALPQPVFWPLLKAITHRHIVSDLEHLVFINTDVGRCRAWLRLALNDGLMECYLKLLLQEPARLCEYYQPTALLRDAEEAEFLLSFLQGLTSLSFELSYKSAILNEWTLTPLSLSGLCPLSELDPLTTSGAELQRKESLDSISHSSGSEDIEVQHSGHKIRRNRKLTASSLSLDTASSSQLSCSLNSDSCLLQENGPKSPDHSEEPMSYDSDLGMANTDDPDRSLQEVLSEFSKAQVNSAPSSGPNQEPDTPMFQTPLSLHSLATSTHLHFEGSEELFPAHKSSGTSSGGHKHQLLPQETPDEKQLGTAQAGPAQSTSDQQPSSPVGGAAGQGSGPWKALEYGRVGPKLVVSSPTSPKGKSWISEDDFCRPPQEPALKSAAGLCTSPVQDTPESRAALHGPFSQGPRKSCSLGALDKACVPSQACGNAQPAPAPAPAPAPAPAPAPGVTQDHKNFCVVHRRQMGLSNPFRGLMKLGTVARRGAMGIWKEFFCELSPLEFRLYLSDEERTCVESCSLLRCEAVGPAHSDGRFELVFSGKKLALRASSQDEAEDWLDRVREALQKVRPQQEDEWVNIQYPDQAEDAPEAPPDSLPPYSTLLPEPAGAQGMQLDWTSAQVPEPDAIKESLLYLYADRTWVPYIFSLSLESLKCFRVRNNEKMLSDSHGVETIRDILPDTSLGGPAFFKIITAKAVLKLQAKNTEEATHWRDLVRKVLASYLESAEEAVTLGGSLDEKCQEVLKFATRENGFLLQYLVAIPTEKGLDSQGCFCAGCSRQIGFSFVRPKLCAFSGLYYCDFCHQDDASVIPARIIHNWDLTKRPVCRQALKFLAQIRAQPLINLQLVNASLYEHVERMHLIGRSREQLKLLGDYLGLCRSGALKELCKRLSHRNYLLESPHRFSVADLQQIAEGVYEGFLKALIEFASQHVYHCDLCTQRGFICQICHHQDIIFPFEFDTTVRCAECRTVFHQSCQAVVRKGCPRCARRRKYQEQNVVS

Target ORF information:

RefSeq Version	NM_183034.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183034.1

ORF Insert Sequence:

ATGCTCTCAG TGGAGAATGG CCTGGACCCT CGTGCTGCCA TCCAGGTCAT CAAGAAGAAG 
CTCGTGGGAT CCGTGAAGGC TCTGCAGAAA CAGCATGTGT CTCTGGACAC AGTGGTCACC 
AGTGAAGATG GAGATGCCAA CACCATGTGC AGTGCCCTGG AGGCTGTGTT CATCCACGGC 
CTGCATGCCA AGCACATCCG CGCCGAGGCT GGCGGCAAAA GGAAGAAGCA CACTCACCAG 
AAGGCTCTGC CTCAGCCTGT CTTCTGGCCC CTTCTGAAAG CCATCACCCA CAGACACATT 
GTCTCAGATT TGGAGCACCT GGTCTTCATC AACACAGATG TAGGCCGTTG CCGAGCCTGG 
CTGCGGTTGG CCCTCAATGA CGGTCTGATG GAGTGCTATC TGAAACTGCT CCTGCAGGAA 
CCTGCACGGT TGTGCGAGTA CTATCAGCCC ACAGCCCTGC TTCGAGATGC CGAGGAGGCT 
GAGTTCCTCC TGAGCTTCCT GCAAGGACTA ACATCCTTGT CCTTCGAACT CTCCTACAAG 
TCAGCCATCT TAAATGAATG GACACTCACC CCATTGTCGC TCTCTGGGCT TTGCCCCCTC 
TCTGAACTCG ACCCTCTCAC TACCTCTGGT GCAGAACTAC AGCGGAAAGA GTCTCTGGAT 
TCAATTTCCC ATTCCTCGGG CTCTGAGGAC ATCGAAGTCC AACACTCAGG CCATAAGATC 
CGCAGGAACA GGAAGCTCAC TGCCTCTTCC CTCAGCCTGG ACACAGCCAG TTCATCCCAG 
CTGTCCTGCA GCCTAAATTC TGACAGCTGC CTGCTCCAAG AGAATGGTCC CAAGAGTCCA 
GACCATTCTG AGGAACCCAT GTCCTATGAC TCAGATCTGG GCATGGCAAA CACCGATGAC 
CCCGACAGGT CTCTGCAAGA GGTGTTGTCG GAATTCAGCA AAGCCCAGGT AAATTCTGCA 
CCAAGCAGCG GACCAAACCA AGAGCCAGAC ACCCCCATGT TCCAGACGCC CCTCTCCCTC 
CATAGCCTAG CTACCAGCAC ACACCTGCAC TTTGAGGGAT CTGAAGAGCT CTTTCCTGCC 
CACAAATCCT CTGGGACATC AAGTGGTGGC CATAAGCATC AGCTACTGCC CCAGGAGACC 
CCAGACGAGA AGCAGCTAGG CACTGCCCAA GCTGGCCCTG CACAGAGCAC TTCAGACCAG 
CAGCCTTCTA GTCCTGTGGG TGGAGCAGCT GGCCAAGGGA GTGGCCCTTG GAAGGCCCTG 
GAGTATGGCA GAGTCGGACC GAAGCTTGTG GTTTCCTCTC CTACGAGTCC GAAAGGCAAG 
AGCTGGATTT CTGAAGATGA CTTCTGCCGA CCTCCCCAGG AGCCTGCTCT AAAGAGCGCT 
GCAGGTCTCT GCACATCTCC TGTGCAAGAC ACTCCAGAGT CAAGGGCTGC TCTCCACGGG 
CCTTTTTCTC AAGGACCAAG AAAGAGCTGT TCCTTGGGGG CATTAGACAA AGCATGTGTG 
CCCTCACAGG CCTGTGGAAA TGCCCAGCCA GCTCCAGCCC CAGCTCCAGC TCCAGCCCCA 
GCCCCAGCCC CAGCTCCAGG GGTGACACAA GACCATAAAA ACTTCTGTGT GGTGCACCGA 
AGGCAAATGG GGCTGTCCAA CCCATTCCGG GGCCTCATGA AGCTGGGCAC AGTGGCCCGG 
CGAGGAGCCA TGGGCATCTG GAAGGAATTC TTTTGTGAGC TGTCCCCGTT GGAGTTCCGC 
CTCTACTTGA GTGATGAGGA ACGCACCTGT GTGGAGAGCT GCTCCCTGCT CCGATGTGAG 
GCTGTGGGGC CAGCACACAG TGATGGACGT TTTGAGTTGG TCTTCTCGGG CAAGAAGCTG 
GCTCTCCGTG CCTCCTCCCA AGACGAGGCT GAGGACTGGC TTGACCGCGT ACGGGAGGCT 
CTGCAGAAGG TGCGCCCTCA GCAGGAAGAT GAGTGGGTGA ATATCCAGTA CCCAGACCAG 
GCTGAGGATG CCCCAGAGGC TCCACCAGAC AGCCTCCCCC CCTATTCAAC TCTACTCCCC 
GAGCCTGCGG GTGCCCAGGG CATGCAGTTG GACTGGACAT CTGCCCAGGT TCCAGAACCA 
GATGCTATCA AAGAGTCCCT TTTGTATCTG TATGCAGACC GGACCTGGGT CCCCTATATC 
TTTTCCCTGT CCTTGGAGTC TCTGAAATGT TTCCGAGTGA GAAACAATGA GAAGATGCTA 
AGTGATAGCC ATGGAGTGGA GACCATCCGA GACATCCTGC CAGACACCAG CCTTGGGGGC 
CCAGCCTTCT TCAAAATCAT CACGGCCAAG GCCGTCCTGA AACTGCAGGC CAAAAACACC 
GAGGAGGCCA CCCACTGGAG AGACCTGGTC CGGAAAGTGC TGGCATCTTA CCTAGAGTCA 
GCTGAGGAGG CTGTGACACT TGGTGGGAGT CTGGATGAAA AATGTCAGGA GGTGCTGAAG 
TTTGCTACCA GGGAGAACGG CTTCCTGCTA CAGTACCTTG TGGCCATCCC GACCGAGAAG 
GGCCTCGACT CCCAGGGCTG CTTCTGCGCA GGCTGCTCCC GACAGATCGG CTTCTCCTTT 
GTACGACCCA AGCTCTGTGC CTTCTCTGGC CTGTATTACT GTGACTTCTG CCACCAAGAC 
GATGCCTCAG TGATTCCAGC CAGAATCATC CACAACTGGG ACCTCACGAA GCGCCCGGTA 
TGCCGGCAGG CCCTGAAGTT CCTGGCCCAG ATCCGGGCCC AACCTCTCAT CAACCTGCAG 
CTGGTGAACG CCTCTCTGTA TGAGCATGTG GAACGCATGC ACCTCATTGG CAGGAGCCGG 
GAGCAGCTGA AACTTCTGGG GGACTACCTG GGCCTGTGCC GAAGTGGTGC TCTGAAGGAG 
TTGTGCAAAA GGCTGAGCCA CAGGAATTAT CTCCTGGAGT CTCCACACAG ATTCAGTGTT 
GCTGACCTCC AGCAGATTGC AGAGGGGGTA TATGAAGGGT TCCTCAAAGC CCTGATTGAA 
TTCGCATCCC AGCATGTCTA CCACTGTGAC CTGTGCACCC AGCGAGGCTT CATCTGCCAG 
ATCTGCCACC ACCAGGACAT CATCTTTCCC TTTGAGTTTG ACACCACAGT CAGGTGTGCT 
GAATGCAGAA CTGTCTTCCA TCAGAGCTGC CAGGCTGTGG TGAGGAAGGG CTGTCCCCGA 
TGTGCCCGCC GGCGCAAGTA CCAGGAACAG AACGTTGTCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plekhm1 ( NM_183034.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_898855.1 Mus musculus pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), mRNA.

Price & Availability↑

CloneID	OMu08322
Clone ID Related Accession (Same CDS sequence)	NM_183034.1 , NM_183034.2
Accession Version	NM_183034.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	pleckstrin homology domain-containing family M member 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY203268.1, BC053079.1, AK048562.1, AL772325.4 and BP767414.1. On Jul 6, 2018 this sequence version replaced NM_183034.1. ##Evidence-Data-START## Transcript exon combination :: BC053079.1, AK155357.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCTCAG TGGAGAATGG CCTGGACCCT CGTGCTGCCA TCCAGGTCAT CAAGAAGAAG 
CTCGTGGGAT CCGTGAAGGC TCTGCAGAAA CAGCATGTGT CTCTGGACAC AGTGGTCACC 
AGTGAAGATG GAGATGCCAA CACCATGTGC AGTGCCCTGG AGGCTGTGTT CATCCACGGC 
CTGCATGCCA AGCACATCCG CGCCGAGGCT GGCGGCAAAA GGAAGAAGCA CACTCACCAG 
AAGGCTCTGC CTCAGCCTGT CTTCTGGCCC CTTCTGAAAG CCATCACCCA CAGACACATT 
GTCTCAGATT TGGAGCACCT GGTCTTCATC AACACAGATG TAGGCCGTTG CCGAGCCTGG 
CTGCGGTTGG CCCTCAATGA CGGTCTGATG GAGTGCTATC TGAAACTGCT CCTGCAGGAA 
CCTGCACGGT TGTGCGAGTA CTATCAGCCC ACAGCCCTGC TTCGAGATGC CGAGGAGGCT 
GAGTTCCTCC TGAGCTTCCT GCAAGGACTA ACATCCTTGT CCTTCGAACT CTCCTACAAG 
TCAGCCATCT TAAATGAATG GACACTCACC CCATTGTCGC TCTCTGGGCT TTGCCCCCTC 
TCTGAACTCG ACCCTCTCAC TACCTCTGGT GCAGAACTAC AGCGGAAAGA GTCTCTGGAT 
TCAATTTCCC ATTCCTCGGG CTCTGAGGAC ATCGAAGTCC AACACTCAGG CCATAAGATC 
CGCAGGAACA GGAAGCTCAC TGCCTCTTCC CTCAGCCTGG ACACAGCCAG TTCATCCCAG 
CTGTCCTGCA GCCTAAATTC TGACAGCTGC CTGCTCCAAG AGAATGGTCC CAAGAGTCCA 
GACCATTCTG AGGAACCCAT GTCCTATGAC TCAGATCTGG GCATGGCAAA CACCGATGAC 
CCCGACAGGT CTCTGCAAGA GGTGTTGTCG GAATTCAGCA AAGCCCAGGT AAATTCTGCA 
CCAAGCAGCG GACCAAACCA AGAGCCAGAC ACCCCCATGT TCCAGACGCC CCTCTCCCTC 
CATAGCCTAG CTACCAGCAC ACACCTGCAC TTTGAGGGAT CTGAAGAGCT CTTTCCTGCC 
CACAAATCCT CTGGGACATC AAGTGGTGGC CATAAGCATC AGCTACTGCC CCAGGAGACC 
CCAGACGAGA AGCAGCTAGG CACTGCCCAA GCTGGCCCTG CACAGAGCAC TTCAGACCAG 
CAGCCTTCTA GTCCTGTGGG TGGAGCAGCT GGCCAAGGGA GTGGCCCTTG GAAGGCCCTG 
GAGTATGGCA GAGTCGGACC GAAGCTTGTG GTTTCCTCTC CTACGAGTCC GAAAGGCAAG 
AGCTGGATTT CTGAAGATGA CTTCTGCCGA CCTCCCCAGG AGCCTGCTCT AAAGAGCGCT 
GCAGGTCTCT GCACATCTCC TGTGCAAGAC ACTCCAGAGT CAAGGGCTGC TCTCCACGGG 
CCTTTTTCTC AAGGACCAAG AAAGAGCTGT TCCTTGGGGG CATTAGACAA AGCATGTGTG 
CCCTCACAGG CCTGTGGAAA TGCCCAGCCA GCTCCAGCCC CAGCTCCAGC TCCAGCCCCA 
GCCCCAGCCC CAGCTCCAGG GGTGACACAA GACCATAAAA ACTTCTGTGT GGTGCACCGA 
AGGCAAATGG GGCTGTCCAA CCCATTCCGG GGCCTCATGA AGCTGGGCAC AGTGGCCCGG 
CGAGGAGCCA TGGGCATCTG GAAGGAATTC TTTTGTGAGC TGTCCCCGTT GGAGTTCCGC 
CTCTACTTGA GTGATGAGGA ACGCACCTGT GTGGAGAGCT GCTCCCTGCT CCGATGTGAG 
GCTGTGGGGC CAGCACACAG TGATGGACGT TTTGAGTTGG TCTTCTCGGG CAAGAAGCTG 
GCTCTCCGTG CCTCCTCCCA AGACGAGGCT GAGGACTGGC TTGACCGCGT ACGGGAGGCT 
CTGCAGAAGG TGCGCCCTCA GCAGGAAGAT GAGTGGGTGA ATATCCAGTA CCCAGACCAG 
GCTGAGGATG CCCCAGAGGC TCCACCAGAC AGCCTCCCCC CCTATTCAAC TCTACTCCCC 
GAGCCTGCGG GTGCCCAGGG CATGCAGTTG GACTGGACAT CTGCCCAGGT TCCAGAACCA 
GATGCTATCA AAGAGTCCCT TTTGTATCTG TATGCAGACC GGACCTGGGT CCCCTATATC 
TTTTCCCTGT CCTTGGAGTC TCTGAAATGT TTCCGAGTGA GAAACAATGA GAAGATGCTA 
AGTGATAGCC ATGGAGTGGA GACCATCCGA GACATCCTGC CAGACACCAG CCTTGGGGGC 
CCAGCCTTCT TCAAAATCAT CACGGCCAAG GCCGTCCTGA AACTGCAGGC CAAAAACACC 
GAGGAGGCCA CCCACTGGAG AGACCTGGTC CGGAAAGTGC TGGCATCTTA CCTAGAGTCA 
GCTGAGGAGG CTGTGACACT TGGTGGGAGT CTGGATGAAA AATGTCAGGA GGTGCTGAAG 
TTTGCTACCA GGGAGAACGG CTTCCTGCTA CAGTACCTTG TGGCCATCCC GACCGAGAAG 
GGCCTCGACT CCCAGGGCTG CTTCTGCGCA GGCTGCTCCC GACAGATCGG CTTCTCCTTT 
GTACGACCCA AGCTCTGTGC CTTCTCTGGC CTGTATTACT GTGACTTCTG CCACCAAGAC 
GATGCCTCAG TGATTCCAGC CAGAATCATC CACAACTGGG ACCTCACGAA GCGCCCGGTA 
TGCCGGCAGG CCCTGAAGTT CCTGGCCCAG ATCCGGGCCC AACCTCTCAT CAACCTGCAG 
CTGGTGAACG CCTCTCTGTA TGAGCATGTG GAACGCATGC ACCTCATTGG CAGGAGCCGG 
GAGCAGCTGA AACTTCTGGG GGACTACCTG GGCCTGTGCC GAAGTGGTGC TCTGAAGGAG 
TTGTGCAAAA GGCTGAGCCA CAGGAATTAT CTCCTGGAGT CTCCACACAG ATTCAGTGTT 
GCTGACCTCC AGCAGATTGC AGAGGGGGTA TATGAAGGGT TCCTCAAAGC CCTGATTGAA 
TTCGCATCCC AGCATGTCTA CCACTGTGAC CTGTGCACCC AGCGAGGCTT CATCTGCCAG 
ATCTGCCACC ACCAGGACAT CATCTTTCCC TTTGAGTTTG ACACCACAGT CAGGTGTGCT 
GAATGCAGAA CTGTCTTCCA TCAGAGCTGC CAGGCTGTGG TGAGGAAGGG CTGTCCCCGA 
TGTGCCCGCC GGCGCAAGTA CCAGGAACAG AACGTTGTCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_898855.1
CDS	155..3379
Translation	MLSVENGLDPRAAIQVIKKKLVGSVKALQKQHVSLDTVVTSEDGDANTMCSALEAVFIHGLHAKHIRAEAGGKRKKHTHQKALPQPVFWPLLKAITHRHIVSDLEHLVFINTDVGRCRAWLRLALNDGLMECYLKLLLQEPARLCEYYQPTALLRDAEEAEFLLSFLQGLTSLSFELSYKSAILNEWTLTPLSLSGLCPLSELDPLTTSGAELQRKESLDSISHSSGSEDIEVQHSGHKIRRNRKLTASSLSLDTASSSQLSCSLNSDSCLLQENGPKSPDHSEEPMSYDSDLGMANTDDPDRSLQEVLSEFSKAQVNSAPSSGPNQEPDTPMFQTPLSLHSLATSTHLHFEGSEELFPAHKSSGTSSGGHKHQLLPQETPDEKQLGTAQAGPAQSTSDQQPSSPVGGAAGQGSGPWKALEYGRVGPKLVVSSPTSPKGKSWISEDDFCRPPQEPALKSAAGLCTSPVQDTPESRAALHGPFSQGPRKSCSLGALDKACVPSQACGNAQPAPAPAPAPAPAPAPAPGVTQDHKNFCVVHRRQMGLSNPFRGLMKLGTVARRGAMGIWKEFFCELSPLEFRLYLSDEERTCVESCSLLRCEAVGPAHSDGRFELVFSGKKLALRASSQDEAEDWLDRVREALQKVRPQQEDEWVNIQYPDQAEDAPEAPPDSLPPYSTLLPEPAGAQGMQLDWTSAQVPEPDAIKESLLYLYADRTWVPYIFSLSLESLKCFRVRNNEKMLSDSHGVETIRDILPDTSLGGPAFFKIITAKAVLKLQAKNTEEATHWRDLVRKVLASYLESAEEAVTLGGSLDEKCQEVLKFATRENGFLLQYLVAIPTEKGLDSQGCFCAGCSRQIGFSFVRPKLCAFSGLYYCDFCHQDDASVIPARIIHNWDLTKRPVCRQALKFLAQIRAQPLINLQLVNASLYEHVERMHLIGRSREQLKLLGDYLGLCRSGALKELCKRLSHRNYLLESPHRFSVADLQQIAEGVYEGFLKALIEFASQHVYHCDLCTQRGFICQICHHQDIIFPFEFDTTVRCAECRTVFHQSCQAVVRKGCPRCARRRKYQEQNVVS

Target ORF information:

RefSeq Version	NM_183034.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183034.2

ORF Insert Sequence:

ATGCTCTCAG TGGAGAATGG CCTGGACCCT CGTGCTGCCA TCCAGGTCAT CAAGAAGAAG 
CTCGTGGGAT CCGTGAAGGC TCTGCAGAAA CAGCATGTGT CTCTGGACAC AGTGGTCACC 
AGTGAAGATG GAGATGCCAA CACCATGTGC AGTGCCCTGG AGGCTGTGTT CATCCACGGC 
CTGCATGCCA AGCACATCCG CGCCGAGGCT GGCGGCAAAA GGAAGAAGCA CACTCACCAG 
AAGGCTCTGC CTCAGCCTGT CTTCTGGCCC CTTCTGAAAG CCATCACCCA CAGACACATT 
GTCTCAGATT TGGAGCACCT GGTCTTCATC AACACAGATG TAGGCCGTTG CCGAGCCTGG 
CTGCGGTTGG CCCTCAATGA CGGTCTGATG GAGTGCTATC TGAAACTGCT CCTGCAGGAA 
CCTGCACGGT TGTGCGAGTA CTATCAGCCC ACAGCCCTGC TTCGAGATGC CGAGGAGGCT 
GAGTTCCTCC TGAGCTTCCT GCAAGGACTA ACATCCTTGT CCTTCGAACT CTCCTACAAG 
TCAGCCATCT TAAATGAATG GACACTCACC CCATTGTCGC TCTCTGGGCT TTGCCCCCTC 
TCTGAACTCG ACCCTCTCAC TACCTCTGGT GCAGAACTAC AGCGGAAAGA GTCTCTGGAT 
TCAATTTCCC ATTCCTCGGG CTCTGAGGAC ATCGAAGTCC AACACTCAGG CCATAAGATC 
CGCAGGAACA GGAAGCTCAC TGCCTCTTCC CTCAGCCTGG ACACAGCCAG TTCATCCCAG 
CTGTCCTGCA GCCTAAATTC TGACAGCTGC CTGCTCCAAG AGAATGGTCC CAAGAGTCCA 
GACCATTCTG AGGAACCCAT GTCCTATGAC TCAGATCTGG GCATGGCAAA CACCGATGAC 
CCCGACAGGT CTCTGCAAGA GGTGTTGTCG GAATTCAGCA AAGCCCAGGT AAATTCTGCA 
CCAAGCAGCG GACCAAACCA AGAGCCAGAC ACCCCCATGT TCCAGACGCC CCTCTCCCTC 
CATAGCCTAG CTACCAGCAC ACACCTGCAC TTTGAGGGAT CTGAAGAGCT CTTTCCTGCC 
CACAAATCCT CTGGGACATC AAGTGGTGGC CATAAGCATC AGCTACTGCC CCAGGAGACC 
CCAGACGAGA AGCAGCTAGG CACTGCCCAA GCTGGCCCTG CACAGAGCAC TTCAGACCAG 
CAGCCTTCTA GTCCTGTGGG TGGAGCAGCT GGCCAAGGGA GTGGCCCTTG GAAGGCCCTG 
GAGTATGGCA GAGTCGGACC GAAGCTTGTG GTTTCCTCTC CTACGAGTCC GAAAGGCAAG 
AGCTGGATTT CTGAAGATGA CTTCTGCCGA CCTCCCCAGG AGCCTGCTCT AAAGAGCGCT 
GCAGGTCTCT GCACATCTCC TGTGCAAGAC ACTCCAGAGT CAAGGGCTGC TCTCCACGGG 
CCTTTTTCTC AAGGACCAAG AAAGAGCTGT TCCTTGGGGG CATTAGACAA AGCATGTGTG 
CCCTCACAGG CCTGTGGAAA TGCCCAGCCA GCTCCAGCCC CAGCTCCAGC TCCAGCCCCA 
GCCCCAGCCC CAGCTCCAGG GGTGACACAA GACCATAAAA ACTTCTGTGT GGTGCACCGA 
AGGCAAATGG GGCTGTCCAA CCCATTCCGG GGCCTCATGA AGCTGGGCAC AGTGGCCCGG 
CGAGGAGCCA TGGGCATCTG GAAGGAATTC TTTTGTGAGC TGTCCCCGTT GGAGTTCCGC 
CTCTACTTGA GTGATGAGGA ACGCACCTGT GTGGAGAGCT GCTCCCTGCT CCGATGTGAG 
GCTGTGGGGC CAGCACACAG TGATGGACGT TTTGAGTTGG TCTTCTCGGG CAAGAAGCTG 
GCTCTCCGTG CCTCCTCCCA AGACGAGGCT GAGGACTGGC TTGACCGCGT ACGGGAGGCT 
CTGCAGAAGG TGCGCCCTCA GCAGGAAGAT GAGTGGGTGA ATATCCAGTA CCCAGACCAG 
GCTGAGGATG CCCCAGAGGC TCCACCAGAC AGCCTCCCCC CCTATTCAAC TCTACTCCCC 
GAGCCTGCGG GTGCCCAGGG CATGCAGTTG GACTGGACAT CTGCCCAGGT TCCAGAACCA 
GATGCTATCA AAGAGTCCCT TTTGTATCTG TATGCAGACC GGACCTGGGT CCCCTATATC 
TTTTCCCTGT CCTTGGAGTC TCTGAAATGT TTCCGAGTGA GAAACAATGA GAAGATGCTA 
AGTGATAGCC ATGGAGTGGA GACCATCCGA GACATCCTGC CAGACACCAG CCTTGGGGGC 
CCAGCCTTCT TCAAAATCAT CACGGCCAAG GCCGTCCTGA AACTGCAGGC CAAAAACACC 
GAGGAGGCCA CCCACTGGAG AGACCTGGTC CGGAAAGTGC TGGCATCTTA CCTAGAGTCA 
GCTGAGGAGG CTGTGACACT TGGTGGGAGT CTGGATGAAA AATGTCAGGA GGTGCTGAAG 
TTTGCTACCA GGGAGAACGG CTTCCTGCTA CAGTACCTTG TGGCCATCCC GACCGAGAAG 
GGCCTCGACT CCCAGGGCTG CTTCTGCGCA GGCTGCTCCC GACAGATCGG CTTCTCCTTT 
GTACGACCCA AGCTCTGTGC CTTCTCTGGC CTGTATTACT GTGACTTCTG CCACCAAGAC 
GATGCCTCAG TGATTCCAGC CAGAATCATC CACAACTGGG ACCTCACGAA GCGCCCGGTA 
TGCCGGCAGG CCCTGAAGTT CCTGGCCCAG ATCCGGGCCC AACCTCTCAT CAACCTGCAG 
CTGGTGAACG CCTCTCTGTA TGAGCATGTG GAACGCATGC ACCTCATTGG CAGGAGCCGG 
GAGCAGCTGA AACTTCTGGG GGACTACCTG GGCCTGTGCC GAAGTGGTGC TCTGAAGGAG 
TTGTGCAAAA GGCTGAGCCA CAGGAATTAT CTCCTGGAGT CTCCACACAG ATTCAGTGTT 
GCTGACCTCC AGCAGATTGC AGAGGGGGTA TATGAAGGGT TCCTCAAAGC CCTGATTGAA 
TTCGCATCCC AGCATGTCTA CCACTGTGAC CTGTGCACCC AGCGAGGCTT CATCTGCCAG 
ATCTGCCACC ACCAGGACAT CATCTTTCCC TTTGAGTTTG ACACCACAGT CAGGTGTGCT 
GAATGCAGAA CTGTCTTCCA TCAGAGCTGC CAGGCTGTGG TGAGGAAGGG CTGTCCCCGA 
TGTGCCCGCC GGCGCAAGTA CCAGGAACAG AACGTTGTCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PLEKHM1/DEF8/RAB7 complex regulates lysosome positioning and bone homeostasis.
JCI insight1(17)e86330(2016 Oct)
Fujiwara T,Ye S,Castro-Gomes T,Winchell CG,Andrews NW,Voth DE,Varughese KI,Mackintosh SG,Feng Y,Pavlos N,Nakamura T,Manolagas SC,Zhao H

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

PLEKHM1: Adapting to life at the lysosome.
Autophagy11(4)720-2(2015 Apr)
McEwan DG,Dikic I

TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates Osteoclast Acidification and Resorption.
PloS one10(5)e0127537(2015)
Witwicka H,Jia H,Kutikov A,Reyes-Gutierrez P,Li X,Odgren PR

High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes.
Bone research214034(2014)
Brommage R,Liu J,Hansen GM,Kirkpatrick LL,Potter DG,Sands AT,Zambrowicz B,Powell DR,Vogel P

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Plekhm1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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