Supv3l1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Supv3l1
Entrez Gene ID	338359
Full Name	suppressor of var1, 3-like 1 (S. cerevisiae)
Synonyms	6330443E10Rik
General protein information	Preferred Names suppressor of var1, 3-like 1 (S. cerevisiae) Names ATP-dependent RNA helicase SUPV3L1, mitochondrial SUV3-like protein 1 suppressor of var1 3-like protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 B4

mRNA and Protein(s)

mRNA	Protein	Name
XM_006513809.1	XP_006513872.1	ATP-dependent RNA helicase SUPV3L1, mitochondrial isoform X1
NM_181423.3	NP_852088.1	ATP-dependent RNA helicase SUPV3L1, mitochondrial isoform 1 precursor
NM_181423.2	NP_852088.1	ATP-dependent RNA helicase SUPV3L1, mitochondrial precursor
NM_001359806.1	NP_001346735.1	ATP-dependent RNA helicase SUPV3L1, mitochondrial isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SUPV3L1	XP_521497.2
Canis lupus familiaris (dog)	SUPV3L1	XP_546136.2
Caenorhabditis elegans (roundworm)	CELE_C08F8.2	NP_001040912.1
Xenopus tropicalis (tropical clawed frog)	supv3l1	XP_002936914.2
	SUPV3L1	XP_001110593.1
Rattus norvegicus (Norway rat)	Supv3l1	NP_001012480.1
Gallus gallus (chicken)	SUPV3L1	NP_001006498.1
Oryza sativa (rice)	Os04g0459800	NP_001052987.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003508	XP_309651.5
Danio rerio (zebrafish)	supv3l1	NP_001077033.1
Drosophila melanogaster (fruit fly)	CG9791	NP_649452.3
Arabidopsis thaliana (thale cress)	AT5G39840	NP_198800.1
Homo sapiens (human)	SUPV3L1	NP_003162.2
Mus musculus (house mouse)	Supv3l1	NP_852088.1

Gene Ontology
Bibliography

Related articles in PubMed

An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers.
Fisher CL, Marks H, Cho LT, Andrews R, Wormald S, Carroll T, Iyer V, Tate P, Rosen B, Stunnenberg HG, Fisher AG, Skarnes WC
Nucleic acids research45(21)e174(2017 Dec)

A staging system for correct phenotype interpretation of mouse embryos harvested on embryonic day 14 (E14.5).
Geyer SH, Reissig L, Rose J, Wilson R, Prin F, Szumska D, Ramirez-Solis R, Tudor C, White J, Mohun TJ, Weninger WJ
Journal of anatomy230(5)710-719(2017 May)

Mitochondrial genome instability resulting from SUV3 haploinsufficiency leads to tumorigenesis and shortened lifespan.
Chen PL, Chen CF, Chen Y, Guo XE, Huang CK, Shew JY, Reddick RL, Wallace DC, Lee WH
Oncogene32(9)1193-201(2013 Feb)

Widespread expression of the Supv3L1 mitochondrial RNA helicase in the mouse.
Paul E, Kielbasinski M, Sedivy JM, Murga-Zamalloa C, Khanna H, Klysik JE
Transgenic research19(4)691-701(2010 Aug)

Disruption of Supv3L1 damages the skin and causes sarcopenia, loss of fat, and death.
Paul E, Cronan R, Weston PJ, Boekelheide K, Sedivy JM, Lee SY, Wiest DL, Resnick MB, Klysik JE
Mammalian genome : official journal of the International Mammalian Genome Society20(2)92-108(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spatial and temporal aspects of Supv3L1 promoter activity.
Title: Widespread expression of the Supv3L1 mitochondrial RNA helicase in the mouse.

Supv3L1 is important for the maintenance of the skin barrier. In addition, loss of Supv3L1 function leads to accelerated aging-like phenotypes.
Title: Disruption of Supv3L1 damages the skin and causes sarcopenia, loss of fat, and death.

Mice homozygous for Supv3L1 mutation die in utero before midgestation.
Title: Interaction of human SUV3 RNA/DNA helicase with BLM helicase; loss of the SUV3 gene results in mouse embryonic lethality.

The following Supv3l1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Supv3l1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu44610	NM_001359806.1 Latest version!	Mus musculus suppressor of var1, 3-like 1 (S. cerevisiae) (Supv3l1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu44610	XM_006513809.1 Latest version!	Mus musculus suppressor of var1, 3-like 1 (S. cerevisiae) (Supv3l1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu08059	NM_181423.3 Latest version!	Mus musculus suppressor of var1, 3-like 1 (S. cerevisiae) (Supv3l1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OMu08059	NM_181423.2	Mus musculus suppressor of var1, 3-like 1 (S. cerevisiae) (Supv3l1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Supv3l1 ( NM_001359806.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346735.1 Mus musculus suppressor of var1, 3-like 1 (S. cerevisiae) (Supv3l1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu44610
Clone ID Related Accession (Same CDS sequence)	XM_006513809.1 , NM_001359806.1
Accession Version	NM_001359806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase SUPV3L1, mitochondrial isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB859685.1, AK143755.1, AK031911.1 and AW049139.1. On Jan 13, 2018 this sequence version replaced XM_006513809.1. ##Evidence-Data-START## Transcript exon combination :: AK031911.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGT CAGTACTCTA CAGGGGTGTG CCGTGTGACT TGGTGACAGG TGAAGAGCGG 
CTGACAGTGG AGCCTGAGGG GAAACAAGCC ACCCATGTCT CTTGCACTGT GGAGATGTGC 
AATGTTGCAA CTCCTTATGA AGTGGCAGTG ATAGATGAAA TCCAGATGAT TCGAGACCCA 
GCCAGAGGAT GGGCTTGGAC CAGAGCACTG CTAGGGCTCT GTGCCGAGGA AGTCCATTTA 
TGTGGAGAGT CTGCTGCTAT TAACCTGGTC TCTGAGCTCC TGTACACCAC TGGGGAGGAA 
GTAGAGGTTC AGAAGTATGA GAGGCTTACC CCCATTTCTG TGCTGGATCA TGCGCTGGAG 
TCTTTGGACA ACCTTCAGCC TGGGGACTGC ATTGTCTGCT TTAGCAAGAA TGACATTTAT 
TCTGTGAGCC GACAGATTGA AATTCGGGGA CTGGAATCCG CGGTGATATA CGGCAGCTTG 
CCACCTGGGA CCAAACTTGC TCAAGCAAGA AAGTTTAATG ACCCCAATGA TCCATGCAAG 
ATCCTGGTAG CCACAGATGC AATTGGCATG GGACTTAATT TGAGCATAAG GAGAATTATT 
TTTTACTCCC TTATAAAGCC CAGCATCAAT GAAAAGGGAG AGAAGGAGCT GGAGCCCATC 
ACCACCTCCC AAGCCCTGCA GATTGCAGGC AGAGCTGGCA GATTCAGCTC ACACTTTAAA 
GAGGGGCAGG TTACCACAAT GCACCGAGAC GACCTGGCTT TATTGAAGGA CATTTTGAAT 
AGACCTGTGG ATCCTATACA GGCCGCCGGT CTCCACCCGA CTGCCGAGCA GATTGAGATG 
TTTGCCTACC ACCTCCCCGA GACAACGCTG TCCAATCTCA TTGATATTTT TGTAGACTTT 
GCACAAGTTG ATGGGCAGTA CTTTGTCTGC AATATGGATG ATTTTAAGTT CTCTGCAGAG 
CTGATCCAGC ATATCCCACT AAGTCTCCGG GTGAGGTATG TGTTCTGCAC AGCCCCCATC 
AATAAGAAGC AGCCTTTTGT CTGCTCGTCG TTGTTACAGT TTGCCAGGCA GTACAGCAGG 
AATGAGCCCC TGACCTTTGC ATGGCTACGC CGGTACATCA AGTGGCCTCT GCTTCCGCCT 
AAGAATATTA AAGACCTCAT GGATCTTGAA GCTGTCCATG ACGTCTTTGA TCTTTACCTG 
TGGCTAAGCT ACCGATTTAT TGATATGTTT CCAGACTCTA GCCTTGTTCG AAGTCTCCAG 
AAAGAGCTGG ATGCCATTAT CCAAGAAGGC GTGCACAACA TCACCAAGCT GATTAAAATC 
TCTGAGTCAC GCAAGCTTTT GAATCTGGAG AGCTTACCAT CGGGGGACCA GTCACGGTTG 
TCAGGAGCCT CCAAGAGCCC AGCGAGAAGG ACACGTGGCA CCAAAAGTGC AGGGAATAAG 
GCCACAGAGC CACTCAGCCC CAGTGACAAG GAGCTGCCCC TTGCCTCTAG GTTGGTGCAG 
CAAGGACTCC TCACTGCAGA CATGCTCAGG CAGCTCCAGA AGGAGTGGCT GACACAGCAG 
CCAGAACACA GCAGAGAGAA AGTGGGGACA CGGAGAAAGA AGAAGGACCC CGACTCCGAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346735.1
CDS	787..2409
Translation	MLLSVLYRGVPCDLVTGEERLTVEPEGKQATHVSCTVEMCNVATPYEVAVIDEIQMIRDPARGWAWTRALLGLCAEEVHLCGESAAINLVSELLYTTGEEVEVQKYERLTPISVLDHALESLDNLQPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQARKFNDPNDPCKILVATDAIGMGLNLSIRRIIFYSLIKPSINEKGEKELEPITTSQALQIAGRAGRFSSHFKEGQVTTMHRDDLALLKDILNRPVDPIQAAGLHPTAEQIEMFAYHLPETTLSNLIDIFVDFAQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVFDLYLWLSYRFIDMFPDSSLVRSLQKELDAIIQEGVHNITKLIKISESRKLLNLESLPSGDQSRLSGASKSPARRTRGTKSAGNKATEPLSPSDKELPLASRLVQQGLLTADMLRQLQKEWLTQQPEHSREKVGTRRKKKDPDSD

Target ORF information:

RefSeq Version	NM_001359806.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus suppressor of var1, 3-like 1 (S. cerevisiae) (Supv3l1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359806.1

ORF Insert Sequence:

ATGCTGCTGT CAGTACTCTA CAGGGGTGTG CCGTGTGACT TGGTGACAGG TGAAGAGCGG 
CTGACAGTGG AGCCTGAGGG GAAACAAGCC ACCCATGTCT CTTGCACTGT GGAGATGTGC 
AATGTTGCAA CTCCTTATGA AGTGGCAGTG ATAGATGAAA TCCAGATGAT TCGAGACCCA 
GCCAGAGGAT GGGCTTGGAC CAGAGCACTG CTAGGGCTCT GTGCCGAGGA AGTCCATTTA 
TGTGGAGAGT CTGCTGCTAT TAACCTGGTC TCTGAGCTCC TGTACACCAC TGGGGAGGAA 
GTAGAGGTTC AGAAGTATGA GAGGCTTACC CCCATTTCTG TGCTGGATCA TGCGCTGGAG 
TCTTTGGACA ACCTTCAGCC TGGGGACTGC ATTGTCTGCT TTAGCAAGAA TGACATTTAT 
TCTGTGAGCC GACAGATTGA AATTCGGGGA CTGGAATCCG CGGTGATATA CGGCAGCTTG 
CCACCTGGGA CCAAACTTGC TCAAGCAAGA AAGTTTAATG ACCCCAATGA TCCATGCAAG 
ATCCTGGTAG CCACAGATGC AATTGGCATG GGACTTAATT TGAGCATAAG GAGAATTATT 
TTTTACTCCC TTATAAAGCC CAGCATCAAT GAAAAGGGAG AGAAGGAGCT GGAGCCCATC 
ACCACCTCCC AAGCCCTGCA GATTGCAGGC AGAGCTGGCA GATTCAGCTC ACACTTTAAA 
GAGGGGCAGG TTACCACAAT GCACCGAGAC GACCTGGCTT TATTGAAGGA CATTTTGAAT 
AGACCTGTGG ATCCTATACA GGCCGCCGGT CTCCACCCGA CTGCCGAGCA GATTGAGATG 
TTTGCCTACC ACCTCCCCGA GACAACGCTG TCCAATCTCA TTGATATTTT TGTAGACTTT 
GCACAAGTTG ATGGGCAGTA CTTTGTCTGC AATATGGATG ATTTTAAGTT CTCTGCAGAG 
CTGATCCAGC ATATCCCACT AAGTCTCCGG GTGAGGTATG TGTTCTGCAC AGCCCCCATC 
AATAAGAAGC AGCCTTTTGT CTGCTCGTCG TTGTTACAGT TTGCCAGGCA GTACAGCAGG 
AATGAGCCCC TGACCTTTGC ATGGCTACGC CGGTACATCA AGTGGCCTCT GCTTCCGCCT 
AAGAATATTA AAGACCTCAT GGATCTTGAA GCTGTCCATG ACGTCTTTGA TCTTTACCTG 
TGGCTAAGCT ACCGATTTAT TGATATGTTT CCAGACTCTA GCCTTGTTCG AAGTCTCCAG 
AAAGAGCTGG ATGCCATTAT CCAAGAAGGC GTGCACAACA TCACCAAGCT GATTAAAATC 
TCTGAGTCAC GCAAGCTTTT GAATCTGGAG AGCTTACCAT CGGGGGACCA GTCACGGTTG 
TCAGGAGCCT CCAAGAGCCC AGCGAGAAGG ACACGTGGCA CCAAAAGTGC AGGGAATAAG 
GCCACAGAGC CACTCAGCCC CAGTGACAAG GAGCTGCCCC TTGCCTCTAG GTTGGTGCAG 
CAAGGACTCC TCACTGCAGA CATGCTCAGG CAGCTCCAGA AGGAGTGGCT GACACAGCAG 
CCAGAACACA GCAGAGAGAA AGTGGGGACA CGGAGAAAGA AGAAGGACCC CGACTCCGAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Supv3l1 ( XM_006513809.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513872.1 Mus musculus suppressor of var1, 3-like 1 (S. cerevisiae) (Supv3l1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu44610
Clone ID Related Accession (Same CDS sequence)	XM_006513809.1 , NM_001359806.1
Accession Version	XM_006513809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase SUPV3L1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGT CAGTACTCTA CAGGGGTGTG CCGTGTGACT TGGTGACAGG TGAAGAGCGG 
CTGACAGTGG AGCCTGAGGG GAAACAAGCC ACCCATGTCT CTTGCACTGT GGAGATGTGC 
AATGTTGCAA CTCCTTATGA AGTGGCAGTG ATAGATGAAA TCCAGATGAT TCGAGACCCA 
GCCAGAGGAT GGGCTTGGAC CAGAGCACTG CTAGGGCTCT GTGCCGAGGA AGTCCATTTA 
TGTGGAGAGT CTGCTGCTAT TAACCTGGTC TCTGAGCTCC TGTACACCAC TGGGGAGGAA 
GTAGAGGTTC AGAAGTATGA GAGGCTTACC CCCATTTCTG TGCTGGATCA TGCGCTGGAG 
TCTTTGGACA ACCTTCAGCC TGGGGACTGC ATTGTCTGCT TTAGCAAGAA TGACATTTAT 
TCTGTGAGCC GACAGATTGA AATTCGGGGA CTGGAATCCG CGGTGATATA CGGCAGCTTG 
CCACCTGGGA CCAAACTTGC TCAAGCAAGA AAGTTTAATG ACCCCAATGA TCCATGCAAG 
ATCCTGGTAG CCACAGATGC AATTGGCATG GGACTTAATT TGAGCATAAG GAGAATTATT 
TTTTACTCCC TTATAAAGCC CAGCATCAAT GAAAAGGGAG AGAAGGAGCT GGAGCCCATC 
ACCACCTCCC AAGCCCTGCA GATTGCAGGC AGAGCTGGCA GATTCAGCTC ACACTTTAAA 
GAGGGGCAGG TTACCACAAT GCACCGAGAC GACCTGGCTT TATTGAAGGA CATTTTGAAT 
AGACCTGTGG ATCCTATACA GGCCGCCGGT CTCCACCCGA CTGCCGAGCA GATTGAGATG 
TTTGCCTACC ACCTCCCCGA GACAACGCTG TCCAATCTCA TTGATATTTT TGTAGACTTT 
GCACAAGTTG ATGGGCAGTA CTTTGTCTGC AATATGGATG ATTTTAAGTT CTCTGCAGAG 
CTGATCCAGC ATATCCCACT AAGTCTCCGG GTGAGGTATG TGTTCTGCAC AGCCCCCATC 
AATAAGAAGC AGCCTTTTGT CTGCTCGTCG TTGTTACAGT TTGCCAGGCA GTACAGCAGG 
AATGAGCCCC TGACCTTTGC ATGGCTACGC CGGTACATCA AGTGGCCTCT GCTTCCGCCT 
AAGAATATTA AAGACCTCAT GGATCTTGAA GCTGTCCATG ACGTCTTTGA TCTTTACCTG 
TGGCTAAGCT ACCGATTTAT TGATATGTTT CCAGACTCTA GCCTTGTTCG AAGTCTCCAG 
AAAGAGCTGG ATGCCATTAT CCAAGAAGGC GTGCACAACA TCACCAAGCT GATTAAAATC 
TCTGAGTCAC GCAAGCTTTT GAATCTGGAG AGCTTACCAT CGGGGGACCA GTCACGGTTG 
TCAGGAGCCT CCAAGAGCCC AGCGAGAAGG ACACGTGGCA CCAAAAGTGC AGGGAATAAG 
GCCACAGAGC CACTCAGCCC CAGTGACAAG GAGCTGCCCC TTGCCTCTAG GTTGGTGCAG 
CAAGGACTCC TCACTGCAGA CATGCTCAGG CAGCTCCAGA AGGAGTGGCT GACACAGCAG 
CCAGAACACA GCAGAGAGAA AGTGGGGACA CGGAGAAAGA AGAAGGACCC CGACTCCGAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513872.1
CDS	732..2354
Translation	MLLSVLYRGVPCDLVTGEERLTVEPEGKQATHVSCTVEMCNVATPYEVAVIDEIQMIRDPARGWAWTRALLGLCAEEVHLCGESAAINLVSELLYTTGEEVEVQKYERLTPISVLDHALESLDNLQPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQARKFNDPNDPCKILVATDAIGMGLNLSIRRIIFYSLIKPSINEKGEKELEPITTSQALQIAGRAGRFSSHFKEGQVTTMHRDDLALLKDILNRPVDPIQAAGLHPTAEQIEMFAYHLPETTLSNLIDIFVDFAQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVFDLYLWLSYRFIDMFPDSSLVRSLQKELDAIIQEGVHNITKLIKISESRKLLNLESLPSGDQSRLSGASKSPARRTRGTKSAGNKATEPLSPSDKELPLASRLVQQGLLTADMLRQLQKEWLTQQPEHSREKVGTRRKKKDPDSD

Target ORF information:

RefSeq Version	XM_006513809.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus suppressor of var1, 3-like 1 (S. cerevisiae) (Supv3l1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513809.1

ORF Insert Sequence:

ATGCTGCTGT CAGTACTCTA CAGGGGTGTG CCGTGTGACT TGGTGACAGG TGAAGAGCGG 
CTGACAGTGG AGCCTGAGGG GAAACAAGCC ACCCATGTCT CTTGCACTGT GGAGATGTGC 
AATGTTGCAA CTCCTTATGA AGTGGCAGTG ATAGATGAAA TCCAGATGAT TCGAGACCCA 
GCCAGAGGAT GGGCTTGGAC CAGAGCACTG CTAGGGCTCT GTGCCGAGGA AGTCCATTTA 
TGTGGAGAGT CTGCTGCTAT TAACCTGGTC TCTGAGCTCC TGTACACCAC TGGGGAGGAA 
GTAGAGGTTC AGAAGTATGA GAGGCTTACC CCCATTTCTG TGCTGGATCA TGCGCTGGAG 
TCTTTGGACA ACCTTCAGCC TGGGGACTGC ATTGTCTGCT TTAGCAAGAA TGACATTTAT 
TCTGTGAGCC GACAGATTGA AATTCGGGGA CTGGAATCCG CGGTGATATA CGGCAGCTTG 
CCACCTGGGA CCAAACTTGC TCAAGCAAGA AAGTTTAATG ACCCCAATGA TCCATGCAAG 
ATCCTGGTAG CCACAGATGC AATTGGCATG GGACTTAATT TGAGCATAAG GAGAATTATT 
TTTTACTCCC TTATAAAGCC CAGCATCAAT GAAAAGGGAG AGAAGGAGCT GGAGCCCATC 
ACCACCTCCC AAGCCCTGCA GATTGCAGGC AGAGCTGGCA GATTCAGCTC ACACTTTAAA 
GAGGGGCAGG TTACCACAAT GCACCGAGAC GACCTGGCTT TATTGAAGGA CATTTTGAAT 
AGACCTGTGG ATCCTATACA GGCCGCCGGT CTCCACCCGA CTGCCGAGCA GATTGAGATG 
TTTGCCTACC ACCTCCCCGA GACAACGCTG TCCAATCTCA TTGATATTTT TGTAGACTTT 
GCACAAGTTG ATGGGCAGTA CTTTGTCTGC AATATGGATG ATTTTAAGTT CTCTGCAGAG 
CTGATCCAGC ATATCCCACT AAGTCTCCGG GTGAGGTATG TGTTCTGCAC AGCCCCCATC 
AATAAGAAGC AGCCTTTTGT CTGCTCGTCG TTGTTACAGT TTGCCAGGCA GTACAGCAGG 
AATGAGCCCC TGACCTTTGC ATGGCTACGC CGGTACATCA AGTGGCCTCT GCTTCCGCCT 
AAGAATATTA AAGACCTCAT GGATCTTGAA GCTGTCCATG ACGTCTTTGA TCTTTACCTG 
TGGCTAAGCT ACCGATTTAT TGATATGTTT CCAGACTCTA GCCTTGTTCG AAGTCTCCAG 
AAAGAGCTGG ATGCCATTAT CCAAGAAGGC GTGCACAACA TCACCAAGCT GATTAAAATC 
TCTGAGTCAC GCAAGCTTTT GAATCTGGAG AGCTTACCAT CGGGGGACCA GTCACGGTTG 
TCAGGAGCCT CCAAGAGCCC AGCGAGAAGG ACACGTGGCA CCAAAAGTGC AGGGAATAAG 
GCCACAGAGC CACTCAGCCC CAGTGACAAG GAGCTGCCCC TTGCCTCTAG GTTGGTGCAG 
CAAGGACTCC TCACTGCAGA CATGCTCAGG CAGCTCCAGA AGGAGTGGCT GACACAGCAG 
CCAGAACACA GCAGAGAGAA AGTGGGGACA CGGAGAAAGA AGAAGGACCC CGACTCCGAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Supv3l1 ( NM_181423.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_852088.1 Mus musculus suppressor of var1, 3-like 1 (S. cerevisiae) (Supv3l1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08059
Clone ID Related Accession (Same CDS sequence)	NM_181423.3 , NM_181423.2
Accession Version	NM_181423.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase SUPV3L1, mitochondrial isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB859685.1, BC049796.1, AW049139.1 and AK143755.1. On Jan 13, 2018 this sequence version replaced NM_181423.2. ##Evidence-Data-START## Transcript exon combination :: AK143755.1, SRR1660817.2943.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCTTGC CTCGGTGTAC CTTGCTGTGG GCTCGGCTCC CGGCGGGGCG CGGGGCTGGG 
CCCCGGGCGG CCCCTTGCTC CGCCCTTCGC GCTCTCGTGG GGTCGTTCCC GGGGGCTTCA 
GGACGCGTTC CCTGCTTGGC CGCCTCCTCC TCTGCCTCTG GTGGCTCCAA AGCCCCGAAC 
ACGTCCTTGT TCGTGCCGCT GACGGTGAAG CCTCAGGGTC CCAGCGCCGA TGGCGACGTG 
GGCGCGGAGC TCACTCGGCC TCTAGACAAG AATGAAGTAA AGAAGATCTT AGACAAGTTT 
TATAAGAGGC AAGAAATTCA AAAGCTGAGT GCTGACTATG GACTTGATGC TCGTCTCTTC 
CACCAGGCTT TCATAAGCTT CAGGAATTAC ATTATGCAAT CCCATTCTCT GGACGTGGAC 
ATTCACATTG TCCTGAATGA TATCTGCTTC AGTGCAGCTC ATGTGGACGA TTTATTTCCA 
TTTTTCTTGA GACATGCCAA ACAAATATTT CCTGTATTGG AATGCAAGGA TGATCTACGT 
AAAATCAGTG ACTTGAGGAT ACCACCCAAC TGGTACCCAG AAGCCAGAGC GAGACAGCGG 
AAGATAATAT TCCACTCAGG CCCTACCAAC AGTGGGAAGA CTTACCATGC CATCCAGAGA 
TACCTGTCAG CGACCTCTGG CGTGTACTGC GGTCCTCTAA AACTGCTGGC GCACGAGATC 
TTTGAGAAGA GCAATGCTGC TGGTGTGCCG TGTGACTTGG TGACAGGTGA AGAGCGGCTG 
ACAGTGGAGC CTGAGGGGAA ACAAGCCACC CATGTCTCTT GCACTGTGGA GATGTGCAAT 
GTTGCAACTC CTTATGAAGT GGCAGTGATA GATGAAATCC AGATGATTCG AGACCCAGCC 
AGAGGATGGG CTTGGACCAG AGCACTGCTA GGGCTCTGTG CCGAGGAAGT CCATTTATGT 
GGAGAGTCTG CTGCTATTAA CCTGGTCTCT GAGCTCCTGT ACACCACTGG GGAGGAAGTA 
GAGGTTCAGA AGTATGAGAG GCTTACCCCC ATTTCTGTGC TGGATCATGC GCTGGAGTCT 
TTGGACAACC TTCAGCCTGG GGACTGCATT GTCTGCTTTA GCAAGAATGA CATTTATTCT 
GTGAGCCGAC AGATTGAAAT TCGGGGACTG GAATCCGCGG TGATATACGG CAGCTTGCCA 
CCTGGGACCA AACTTGCTCA AGCAAGAAAG TTTAATGACC CCAATGATCC ATGCAAGATC 
CTGGTAGCCA CAGATGCAAT TGGCATGGGA CTTAATTTGA GCATAAGGAG AATTATTTTT 
TACTCCCTTA TAAAGCCCAG CATCAATGAA AAGGGAGAGA AGGAGCTGGA GCCCATCACC 
ACCTCCCAAG CCCTGCAGAT TGCAGGCAGA GCTGGCAGAT TCAGCTCACA CTTTAAAGAG 
GGGCAGGTTA CCACAATGCA CCGAGACGAC CTGGCTTTAT TGAAGGACAT TTTGAATAGA 
CCTGTGGATC CTATACAGGC CGCCGGTCTC CACCCGACTG CCGAGCAGAT TGAGATGTTT 
GCCTACCACC TCCCCGAGAC AACGCTGTCC AATCTCATTG ATATTTTTGT AGACTTTGCA 
CAAGTTGATG GGCAGTACTT TGTCTGCAAT ATGGATGATT TTAAGTTCTC TGCAGAGCTG 
ATCCAGCATA TCCCACTAAG TCTCCGGGTG AGGTATGTGT TCTGCACAGC CCCCATCAAT 
AAGAAGCAGC CTTTTGTCTG CTCGTCGTTG TTACAGTTTG CCAGGCAGTA CAGCAGGAAT 
GAGCCCCTGA CCTTTGCATG GCTACGCCGG TACATCAAGT GGCCTCTGCT TCCGCCTAAG 
AATATTAAAG ACCTCATGGA TCTTGAAGCT GTCCATGACG TCTTTGATCT TTACCTGTGG 
CTAAGCTACC GATTTATTGA TATGTTTCCA GACTCTAGCC TTGTTCGAAG TCTCCAGAAA 
GAGCTGGATG CCATTATCCA AGAAGGCGTG CACAACATCA CCAAGCTGAT TAAAATCTCT 
GAGTCACGCA AGCTTTTGAA TCTGGAGAGC TTACCATCGG GGGACCAGTC ACGGTTGTCA 
GGAGCCTCCA AGAGCCCAGC GAGAAGGACA CGTGGCACCA AAAGTGCAGG GAATAAGGCC 
ACAGAGCCAC TCAGCCCCAG TGACAAGGAG CTGCCCCTTG CCTCTAGGTT GGTGCAGCAA 
GGACTCCTCA CTGCAGACAT GCTCAGGCAG CTCCAGAAGG AGTGGCTGAC ACAGCAGCCA 
GAACACAGCA GAGAGAAAGT GGGGACACGG AGAAAGAAGA AGGACCCCGA CTCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852088.1
CDS	54..2393
Translation	MSLPRCTLLWARLPAGRGAGPRAAPCSALRALVGSFPGASGRVPCLAASSSASGGSKAPNTSLFVPLTVKPQGPSADGDVGAELTRPLDKNEVKKILDKFYKRQEIQKLSADYGLDARLFHQAFISFRNYIMQSHSLDVDIHIVLNDICFSAAHVDDLFPFFLRHAKQIFPVLECKDDLRKISDLRIPPNWYPEARARQRKIIFHSGPTNSGKTYHAIQRYLSATSGVYCGPLKLLAHEIFEKSNAAGVPCDLVTGEERLTVEPEGKQATHVSCTVEMCNVATPYEVAVIDEIQMIRDPARGWAWTRALLGLCAEEVHLCGESAAINLVSELLYTTGEEVEVQKYERLTPISVLDHALESLDNLQPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQARKFNDPNDPCKILVATDAIGMGLNLSIRRIIFYSLIKPSINEKGEKELEPITTSQALQIAGRAGRFSSHFKEGQVTTMHRDDLALLKDILNRPVDPIQAAGLHPTAEQIEMFAYHLPETTLSNLIDIFVDFAQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVFDLYLWLSYRFIDMFPDSSLVRSLQKELDAIIQEGVHNITKLIKISESRKLLNLESLPSGDQSRLSGASKSPARRTRGTKSAGNKATEPLSPSDKELPLASRLVQQGLLTADMLRQLQKEWLTQQPEHSREKVGTRRKKKDPDSD

Target ORF information:

RefSeq Version	NM_181423.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus suppressor of var1, 3-like 1 (S. cerevisiae) (Supv3l1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181423.3

ORF Insert Sequence:

ATGTCCTTGC CTCGGTGTAC CTTGCTGTGG GCTCGGCTCC CGGCGGGGCG CGGGGCTGGG 
CCCCGGGCGG CCCCTTGCTC CGCCCTTCGC GCTCTCGTGG GGTCGTTCCC GGGGGCTTCA 
GGACGCGTTC CCTGCTTGGC CGCCTCCTCC TCTGCCTCTG GTGGCTCCAA AGCCCCGAAC 
ACGTCCTTGT TCGTGCCGCT GACGGTGAAG CCTCAGGGTC CCAGCGCCGA TGGCGACGTG 
GGCGCGGAGC TCACTCGGCC TCTAGACAAG AATGAAGTAA AGAAGATCTT AGACAAGTTT 
TATAAGAGGC AAGAAATTCA AAAGCTGAGT GCTGACTATG GACTTGATGC TCGTCTCTTC 
CACCAGGCTT TCATAAGCTT CAGGAATTAC ATTATGCAAT CCCATTCTCT GGACGTGGAC 
ATTCACATTG TCCTGAATGA TATCTGCTTC AGTGCAGCTC ATGTGGACGA TTTATTTCCA 
TTTTTCTTGA GACATGCCAA ACAAATATTT CCTGTATTGG AATGCAAGGA TGATCTACGT 
AAAATCAGTG ACTTGAGGAT ACCACCCAAC TGGTACCCAG AAGCCAGAGC GAGACAGCGG 
AAGATAATAT TCCACTCAGG CCCTACCAAC AGTGGGAAGA CTTACCATGC CATCCAGAGA 
TACCTGTCAG CGACCTCTGG CGTGTACTGC GGTCCTCTAA AACTGCTGGC GCACGAGATC 
TTTGAGAAGA GCAATGCTGC TGGTGTGCCG TGTGACTTGG TGACAGGTGA AGAGCGGCTG 
ACAGTGGAGC CTGAGGGGAA ACAAGCCACC CATGTCTCTT GCACTGTGGA GATGTGCAAT 
GTTGCAACTC CTTATGAAGT GGCAGTGATA GATGAAATCC AGATGATTCG AGACCCAGCC 
AGAGGATGGG CTTGGACCAG AGCACTGCTA GGGCTCTGTG CCGAGGAAGT CCATTTATGT 
GGAGAGTCTG CTGCTATTAA CCTGGTCTCT GAGCTCCTGT ACACCACTGG GGAGGAAGTA 
GAGGTTCAGA AGTATGAGAG GCTTACCCCC ATTTCTGTGC TGGATCATGC GCTGGAGTCT 
TTGGACAACC TTCAGCCTGG GGACTGCATT GTCTGCTTTA GCAAGAATGA CATTTATTCT 
GTGAGCCGAC AGATTGAAAT TCGGGGACTG GAATCCGCGG TGATATACGG CAGCTTGCCA 
CCTGGGACCA AACTTGCTCA AGCAAGAAAG TTTAATGACC CCAATGATCC ATGCAAGATC 
CTGGTAGCCA CAGATGCAAT TGGCATGGGA CTTAATTTGA GCATAAGGAG AATTATTTTT 
TACTCCCTTA TAAAGCCCAG CATCAATGAA AAGGGAGAGA AGGAGCTGGA GCCCATCACC 
ACCTCCCAAG CCCTGCAGAT TGCAGGCAGA GCTGGCAGAT TCAGCTCACA CTTTAAAGAG 
GGGCAGGTTA CCACAATGCA CCGAGACGAC CTGGCTTTAT TGAAGGACAT TTTGAATAGA 
CCTGTGGATC CTATACAGGC CGCCGGTCTC CACCCGACTG CCGAGCAGAT TGAGATGTTT 
GCCTACCACC TCCCCGAGAC AACGCTGTCC AATCTCATTG ATATTTTTGT AGACTTTGCA 
CAAGTTGATG GGCAGTACTT TGTCTGCAAT ATGGATGATT TTAAGTTCTC TGCAGAGCTG 
ATCCAGCATA TCCCACTAAG TCTCCGGGTG AGGTATGTGT TCTGCACAGC CCCCATCAAT 
AAGAAGCAGC CTTTTGTCTG CTCGTCGTTG TTACAGTTTG CCAGGCAGTA CAGCAGGAAT 
GAGCCCCTGA CCTTTGCATG GCTACGCCGG TACATCAAGT GGCCTCTGCT TCCGCCTAAG 
AATATTAAAG ACCTCATGGA TCTTGAAGCT GTCCATGACG TCTTTGATCT TTACCTGTGG 
CTAAGCTACC GATTTATTGA TATGTTTCCA GACTCTAGCC TTGTTCGAAG TCTCCAGAAA 
GAGCTGGATG CCATTATCCA AGAAGGCGTG CACAACATCA CCAAGCTGAT TAAAATCTCT 
GAGTCACGCA AGCTTTTGAA TCTGGAGAGC TTACCATCGG GGGACCAGTC ACGGTTGTCA 
GGAGCCTCCA AGAGCCCAGC GAGAAGGACA CGTGGCACCA AAAGTGCAGG GAATAAGGCC 
ACAGAGCCAC TCAGCCCCAG TGACAAGGAG CTGCCCCTTG CCTCTAGGTT GGTGCAGCAA 
GGACTCCTCA CTGCAGACAT GCTCAGGCAG CTCCAGAAGG AGTGGCTGAC ACAGCAGCCA 
GAACACAGCA GAGAGAAAGT GGGGACACGG AGAAAGAAGA AGGACCCCGA CTCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Supv3l1 ( NM_181423.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_852088.1 Mus musculus suppressor of var1, 3-like 1 (S. cerevisiae) (Supv3l1), mRNA.

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CloneID	OMu08059
Clone ID Related Accession (Same CDS sequence)	NM_181423.3 , NM_181423.2
Accession Version	NM_181423.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase SUPV3L1, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC049796.1. On Jun 3, 2003 this sequence version replaced NM_181423.1. ##Evidence-Data-START## Transcript exon combination :: BC049796.1, AJ968954.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTCCTTGC CTCGGTGTAC CTTGCTGTGG GCTCGGCTCC CGGCGGGGCG CGGGGCTGGG 
CCCCGGGCGG CCCCTTGCTC CGCCCTTCGC GCTCTCGTGG GGTCGTTCCC GGGGGCTTCA 
GGACGCGTTC CCTGCTTGGC CGCCTCCTCC TCTGCCTCTG GTGGCTCCAA AGCCCCGAAC 
ACGTCCTTGT TCGTGCCGCT GACGGTGAAG CCTCAGGGTC CCAGCGCCGA TGGCGACGTG 
GGCGCGGAGC TCACTCGGCC TCTAGACAAG AATGAAGTAA AGAAGATCTT AGACAAGTTT 
TATAAGAGGC AAGAAATTCA AAAGCTGAGT GCTGACTATG GACTTGATGC TCGTCTCTTC 
CACCAGGCTT TCATAAGCTT CAGGAATTAC ATTATGCAAT CCCATTCTCT GGACGTGGAC 
ATTCACATTG TCCTGAATGA TATCTGCTTC AGTGCAGCTC ATGTGGACGA TTTATTTCCA 
TTTTTCTTGA GACATGCCAA ACAAATATTT CCTGTATTGG AATGCAAGGA TGATCTACGT 
AAAATCAGTG ACTTGAGGAT ACCACCCAAC TGGTACCCAG AAGCCAGAGC GAGACAGCGG 
AAGATAATAT TCCACTCAGG CCCTACCAAC AGTGGGAAGA CTTACCATGC CATCCAGAGA 
TACCTGTCAG CGACCTCTGG CGTGTACTGC GGTCCTCTAA AACTGCTGGC GCACGAGATC 
TTTGAGAAGA GCAATGCTGC TGGTGTGCCG TGTGACTTGG TGACAGGTGA AGAGCGGCTG 
ACAGTGGAGC CTGAGGGGAA ACAAGCCACC CATGTCTCTT GCACTGTGGA GATGTGCAAT 
GTTGCAACTC CTTATGAAGT GGCAGTGATA GATGAAATCC AGATGATTCG AGACCCAGCC 
AGAGGATGGG CTTGGACCAG AGCACTGCTA GGGCTCTGTG CCGAGGAAGT CCATTTATGT 
GGAGAGTCTG CTGCTATTAA CCTGGTCTCT GAGCTCCTGT ACACCACTGG GGAGGAAGTA 
GAGGTTCAGA AGTATGAGAG GCTTACCCCC ATTTCTGTGC TGGATCATGC GCTGGAGTCT 
TTGGACAACC TTCAGCCTGG GGACTGCATT GTCTGCTTTA GCAAGAATGA CATTTATTCT 
GTGAGCCGAC AGATTGAAAT TCGGGGACTG GAATCCGCGG TGATATACGG CAGCTTGCCA 
CCTGGGACCA AACTTGCTCA AGCAAGAAAG TTTAATGACC CCAATGATCC ATGCAAGATC 
CTGGTAGCCA CAGATGCAAT TGGCATGGGA CTTAATTTGA GCATAAGGAG AATTATTTTT 
TACTCCCTTA TAAAGCCCAG CATCAATGAA AAGGGAGAGA AGGAGCTGGA GCCCATCACC 
ACCTCCCAAG CCCTGCAGAT TGCAGGCAGA GCTGGCAGAT TCAGCTCACA CTTTAAAGAG 
GGGCAGGTTA CCACAATGCA CCGAGACGAC CTGGCTTTAT TGAAGGACAT TTTGAATAGA 
CCTGTGGATC CTATACAGGC CGCCGGTCTC CACCCGACTG CCGAGCAGAT TGAGATGTTT 
GCCTACCACC TCCCCGAGAC AACGCTGTCC AATCTCATTG ATATTTTTGT AGACTTTGCA 
CAAGTTGATG GGCAGTACTT TGTCTGCAAT ATGGATGATT TTAAGTTCTC TGCAGAGCTG 
ATCCAGCATA TCCCACTAAG TCTCCGGGTG AGGTATGTGT TCTGCACAGC CCCCATCAAT 
AAGAAGCAGC CTTTTGTCTG CTCGTCGTTG TTACAGTTTG CCAGGCAGTA CAGCAGGAAT 
GAGCCCCTGA CCTTTGCATG GCTACGCCGG TACATCAAGT GGCCTCTGCT TCCGCCTAAG 
AATATTAAAG ACCTCATGGA TCTTGAAGCT GTCCATGACG TCTTTGATCT TTACCTGTGG 
CTAAGCTACC GATTTATTGA TATGTTTCCA GACTCTAGCC TTGTTCGAAG TCTCCAGAAA 
GAGCTGGATG CCATTATCCA AGAAGGCGTG CACAACATCA CCAAGCTGAT TAAAATCTCT 
GAGTCACGCA AGCTTTTGAA TCTGGAGAGC TTACCATCGG GGGACCAGTC ACGGTTGTCA 
GGAGCCTCCA AGAGCCCAGC GAGAAGGACA CGTGGCACCA AAAGTGCAGG GAATAAGGCC 
ACAGAGCCAC TCAGCCCCAG TGACAAGGAG CTGCCCCTTG CCTCTAGGTT GGTGCAGCAA 
GGACTCCTCA CTGCAGACAT GCTCAGGCAG CTCCAGAAGG AGTGGCTGAC ACAGCAGCCA 
GAACACAGCA GAGAGAAAGT GGGGACACGG AGAAAGAAGA AGGACCCCGA CTCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852088.1
CDS	9..2348
Translation	MSLPRCTLLWARLPAGRGAGPRAAPCSALRALVGSFPGASGRVPCLAASSSASGGSKAPNTSLFVPLTVKPQGPSADGDVGAELTRPLDKNEVKKILDKFYKRQEIQKLSADYGLDARLFHQAFISFRNYIMQSHSLDVDIHIVLNDICFSAAHVDDLFPFFLRHAKQIFPVLECKDDLRKISDLRIPPNWYPEARARQRKIIFHSGPTNSGKTYHAIQRYLSATSGVYCGPLKLLAHEIFEKSNAAGVPCDLVTGEERLTVEPEGKQATHVSCTVEMCNVATPYEVAVIDEIQMIRDPARGWAWTRALLGLCAEEVHLCGESAAINLVSELLYTTGEEVEVQKYERLTPISVLDHALESLDNLQPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQARKFNDPNDPCKILVATDAIGMGLNLSIRRIIFYSLIKPSINEKGEKELEPITTSQALQIAGRAGRFSSHFKEGQVTTMHRDDLALLKDILNRPVDPIQAAGLHPTAEQIEMFAYHLPETTLSNLIDIFVDFAQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVFDLYLWLSYRFIDMFPDSSLVRSLQKELDAIIQEGVHNITKLIKISESRKLLNLESLPSGDQSRLSGASKSPARRTRGTKSAGNKATEPLSPSDKELPLASRLVQQGLLTADMLRQLQKEWLTQQPEHSREKVGTRRKKKDPDSD

Target ORF information:

RefSeq Version	NM_181423.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus suppressor of var1, 3-like 1 (S. cerevisiae) (Supv3l1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181423.2

ORF Insert Sequence:

ATGTCCTTGC CTCGGTGTAC CTTGCTGTGG GCTCGGCTCC CGGCGGGGCG CGGGGCTGGG 
CCCCGGGCGG CCCCTTGCTC CGCCCTTCGC GCTCTCGTGG GGTCGTTCCC GGGGGCTTCA 
GGACGCGTTC CCTGCTTGGC CGCCTCCTCC TCTGCCTCTG GTGGCTCCAA AGCCCCGAAC 
ACGTCCTTGT TCGTGCCGCT GACGGTGAAG CCTCAGGGTC CCAGCGCCGA TGGCGACGTG 
GGCGCGGAGC TCACTCGGCC TCTAGACAAG AATGAAGTAA AGAAGATCTT AGACAAGTTT 
TATAAGAGGC AAGAAATTCA AAAGCTGAGT GCTGACTATG GACTTGATGC TCGTCTCTTC 
CACCAGGCTT TCATAAGCTT CAGGAATTAC ATTATGCAAT CCCATTCTCT GGACGTGGAC 
ATTCACATTG TCCTGAATGA TATCTGCTTC AGTGCAGCTC ATGTGGACGA TTTATTTCCA 
TTTTTCTTGA GACATGCCAA ACAAATATTT CCTGTATTGG AATGCAAGGA TGATCTACGT 
AAAATCAGTG ACTTGAGGAT ACCACCCAAC TGGTACCCAG AAGCCAGAGC GAGACAGCGG 
AAGATAATAT TCCACTCAGG CCCTACCAAC AGTGGGAAGA CTTACCATGC CATCCAGAGA 
TACCTGTCAG CGACCTCTGG CGTGTACTGC GGTCCTCTAA AACTGCTGGC GCACGAGATC 
TTTGAGAAGA GCAATGCTGC TGGTGTGCCG TGTGACTTGG TGACAGGTGA AGAGCGGCTG 
ACAGTGGAGC CTGAGGGGAA ACAAGCCACC CATGTCTCTT GCACTGTGGA GATGTGCAAT 
GTTGCAACTC CTTATGAAGT GGCAGTGATA GATGAAATCC AGATGATTCG AGACCCAGCC 
AGAGGATGGG CTTGGACCAG AGCACTGCTA GGGCTCTGTG CCGAGGAAGT CCATTTATGT 
GGAGAGTCTG CTGCTATTAA CCTGGTCTCT GAGCTCCTGT ACACCACTGG GGAGGAAGTA 
GAGGTTCAGA AGTATGAGAG GCTTACCCCC ATTTCTGTGC TGGATCATGC GCTGGAGTCT 
TTGGACAACC TTCAGCCTGG GGACTGCATT GTCTGCTTTA GCAAGAATGA CATTTATTCT 
GTGAGCCGAC AGATTGAAAT TCGGGGACTG GAATCCGCGG TGATATACGG CAGCTTGCCA 
CCTGGGACCA AACTTGCTCA AGCAAGAAAG TTTAATGACC CCAATGATCC ATGCAAGATC 
CTGGTAGCCA CAGATGCAAT TGGCATGGGA CTTAATTTGA GCATAAGGAG AATTATTTTT 
TACTCCCTTA TAAAGCCCAG CATCAATGAA AAGGGAGAGA AGGAGCTGGA GCCCATCACC 
ACCTCCCAAG CCCTGCAGAT TGCAGGCAGA GCTGGCAGAT TCAGCTCACA CTTTAAAGAG 
GGGCAGGTTA CCACAATGCA CCGAGACGAC CTGGCTTTAT TGAAGGACAT TTTGAATAGA 
CCTGTGGATC CTATACAGGC CGCCGGTCTC CACCCGACTG CCGAGCAGAT TGAGATGTTT 
GCCTACCACC TCCCCGAGAC AACGCTGTCC AATCTCATTG ATATTTTTGT AGACTTTGCA 
CAAGTTGATG GGCAGTACTT TGTCTGCAAT ATGGATGATT TTAAGTTCTC TGCAGAGCTG 
ATCCAGCATA TCCCACTAAG TCTCCGGGTG AGGTATGTGT TCTGCACAGC CCCCATCAAT 
AAGAAGCAGC CTTTTGTCTG CTCGTCGTTG TTACAGTTTG CCAGGCAGTA CAGCAGGAAT 
GAGCCCCTGA CCTTTGCATG GCTACGCCGG TACATCAAGT GGCCTCTGCT TCCGCCTAAG 
AATATTAAAG ACCTCATGGA TCTTGAAGCT GTCCATGACG TCTTTGATCT TTACCTGTGG 
CTAAGCTACC GATTTATTGA TATGTTTCCA GACTCTAGCC TTGTTCGAAG TCTCCAGAAA 
GAGCTGGATG CCATTATCCA AGAAGGCGTG CACAACATCA CCAAGCTGAT TAAAATCTCT 
GAGTCACGCA AGCTTTTGAA TCTGGAGAGC TTACCATCGG GGGACCAGTC ACGGTTGTCA 
GGAGCCTCCA AGAGCCCAGC GAGAAGGACA CGTGGCACCA AAAGTGCAGG GAATAAGGCC 
ACAGAGCCAC TCAGCCCCAG TGACAAGGAG CTGCCCCTTG CCTCTAGGTT GGTGCAGCAA 
GGACTCCTCA CTGCAGACAT GCTCAGGCAG CTCCAGAAGG AGTGGCTGAC ACAGCAGCCA 
GAACACAGCA GAGAGAAAGT GGGGACACGG AGAAAGAAGA AGGACCCCGA CTCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers.
Nucleic acids research45(21)e174(2017 Dec)
Fisher CL,Marks H,Cho LT,Andrews R,Wormald S,Carroll T,Iyer V,Tate P,Rosen B,Stunnenberg HG,Fisher AG,Skarnes WC

A staging system for correct phenotype interpretation of mouse embryos harvested on embryonic day 14 (E14.5).
Journal of anatomy230(5)710-719(2017 May)
Geyer SH,Reissig L,Rose J,Wilson R,Prin F,Szumska D,Ramirez-Solis R,Tudor C,White J,Mohun TJ,Weninger WJ

Mitochondrial genome instability resulting from SUV3 haploinsufficiency leads to tumorigenesis and shortened lifespan.
Oncogene32(9)1193-201(2013 Feb)
Chen PL,Chen CF,Chen Y,Guo XE,Huang CK,Shew JY,Reddick RL,Wallace DC,Lee WH

Widespread expression of the Supv3L1 mitochondrial RNA helicase in the mouse.
Transgenic research19(4)691-701(2010 Aug)
Paul E,Kielbasinski M,Sedivy JM,Murga-Zamalloa C,Khanna H,Klysik JE

Disruption of Supv3L1 damages the skin and causes sarcopenia, loss of fat, and death.
Mammalian genome : official journal of the International Mammalian Genome Society20(2)92-108(2009 Feb)
Paul E,Cronan R,Weston PJ,Boekelheide K,Sedivy JM,Lee SY,Wiest DL,Resnick MB,Klysik JE

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Supv3l1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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