Ksr2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ksr2
Entrez Gene ID	333050
Full Name	kinase suppressor of ras 2
General protein information	Preferred Names kinase suppressor of ras 2 Names kinase suppressor of Ras 2 testes-kinase suppressor of ras 2 isoform
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
XM_006530394.3	XP_006530457.1	kinase suppressor of Ras 2 isoform X2
XM_006530395.4	XP_006530458.1	kinase suppressor of Ras 2 isoform X3
XM_006530393.3	XP_006530456.1	kinase suppressor of Ras 2 isoform X1
NM_001312914.1	NP_001299843.1	kinase suppressor of Ras 2
XM_006530393.4	XP_006530456.1	kinase suppressor of Ras 2 isoform X1
XM_006530395.3	XP_006530458.1	kinase suppressor of Ras 2 isoform X3
XM_006530394.4	XP_006530457.1	kinase suppressor of Ras 2 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ksr2	NP_001108017.2
Canis lupus familiaris (dog)	KSR2	XP_543417.3
Bos taurus (cattle)	KSR2	NP_001193791.1
Xenopus tropicalis (tropical clawed frog)	ksr2	XP_004910557.1
	KSR2	XP_001083311.2
Homo sapiens (human)	KSR2	NP_775869.3
Pan troglodytes (chimpanzee)	KSR2	XP_003952358.1
Rattus norvegicus (Norway rat)	Ksr2	XP_006249546.1
Danio rerio (zebrafish)	ksr2	NP_001137513.1

Gene Ontology
Bibliography

Related articles in PubMed

Kinase Suppressor of Ras 2 (KSR2) expression in the brain regulates energy balance and glucose homeostasis.
Guo L, Costanzo-Garvey DL, Smith DR, Neilsen BK, MacDonald RG, Lewis RE
Molecular metabolism6(2)194-205(2017 Feb)

Cell non-autonomous regulation of hepatic IGF-1 and neonatal growth by Kinase Suppressor of Ras 2 (KSR2).
Guo L, Costanzo-Garvey DL, Smith DR, Zavorka ME, Venable-Kang M, MacDonald RG, Lewis RE
Scientific reports632093(2016 Aug)

Ultrastructural study of spermatogenesis in KSR2 deficient mice.
Moretti E, Collodel G, Mazzi L, Russo I, Giurisato E
Transgenic research24(4)741-51(2015 Aug)

Identification of a truncated kinase suppressor of Ras 2 mRNA in sperm.
Guo L, Volle DJ, Lewis RE
FEBS open bio4420-5(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

KSR2 deficiency affects stromal interaction molecule 1 (STIM1)/ORAI1 puncta formation, which is correlated with cytoskeleton disorganization.
Title: The KSR2-calcineurin complex regulates STIM1-ORAI1 dynamics and store-operated calcium entry (SOCE).

KSR2 is essential to tumor cell energy homeostasis and critical to the integration of mitogenic and metabolic signaling pathways
Title: Kinase suppressor of Ras 2 (KSR2) regulates tumor cell transformation via AMPK.

The metabolic phenotype of KSR2 heterozygous (KSR2(+/minus;)) and KSR2(-/-) mice suggests that human KSR2 variants may contribute to a similar phenotype linked to human chromosome 12q24
Title: Profound obesity secondary to hyperphagia in mice lacking kinase suppressor of ras 2.

KSR2 regulates AMPK-dependent glucose uptake and fatty acid oxidation in mouse embryonic fibroblasts and glycolysis in a neuronal cell line.
Title: KSR2 is an essential regulator of AMP kinase, energy expenditure, and insulin sensitivity.

The protein phosphatase calcineurin selectively interacts with KSR2 and KSR2 uniquely contributes to Ca2+-mediated ERK signaling.
Title: KSR2 is a calcineurin substrate that promotes ERK cascade activation in response to calcium signals.

The following Ksr2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ksr2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu74925	NM_001312914.1 Latest version!	Mus musculus kinase suppressor of ras 2 (Ksr2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu35946	XM_006530393.4 Latest version!	Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu35947	XM_006530394.4 Latest version!	Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu35948	XM_006530395.4 Latest version!	Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu35946	XM_006530393.3	Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu35948	XM_006530395.3	Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu35947	XM_006530394.3	Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ksr2 ( XM_006530394.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530457.1 Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu35947
Clone ID Related Accession (Same CDS sequence)	XM_006530394.3 , XM_006530394.4
Accession Version	XM_006530394.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinase suppressor of Ras 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530394.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGCA CCCTGTGGAG GTTGGTCCTC AGCCCTAGTC CCAAGCCTCC TTCAGAGAGT 
CTACACCAGG CCCACCACAG AGGCGAGAGA ATTTTTGGAG CCAAGAAGAA GAGCAAACCC 
TTGAACCTCA AGATCCACAG CGGCGTGGGC AGCTGCGAGA ACATCCCCGC CCAGCAACGC 
TCTCCGCTCC TGTCCGAGCG CTCCTTGCGG TCCTTCTTTG TGGGCCACGG GCCCTTCCTG 
CCCTCCACCC CACCTGTCCA CACTGAAGCC AATTTCTCTG CAAACACACT CTCTGTACCT 
CGCTGGTCAC CACAGATCCC ACGCAGAGAC CTTGGCAATT CCATCAAGCA CAGGTTTTCC 
ACCAAGTACT GGATGTCTCA GACGTGCACG GTCTGCGGGA AAGGGATGCT TTTTGGCCTC 
AAGTGTAAAA ACTGCAAGTT AAAGTGCCAC AATAAATGCA CCAAAGAAGC CCCGCCCTGT 
CACCTCCTGA TTATCCACCG AGGAGACCCA GCAAGGTTAG TGCGAACGGA GTCGGTGCCC 
TGTGACATCA ACAACCCGGT CCGGAAGCCT GCCCGATATT CAGATCTCCA CATCAGTCAG 
ACTCTCCCCA AAACCAACAA AATCAACAAG GACCACATCC CTGTCCCTTA CCAGCCAGAC 
TCCAGCAGCA ATCCCTCCTC CACGACGTCC TCCACGCCCT CCTCACCGGC ACCCCCCCTC 
CCTCCCAGCG CCACGCCGCC TTCTCCCCTG CACCCCTCCC CGCAGTGCCC GAGACAGAAA 
AAGAACTTCA ACCTGCCAGC TTCCCACTAC TACAAATACA AGCAACAGTT CATCTTCCCA 
GATGTGGTGC CTGTACCAGA GACACCCACC CGGGCACCCC AAGTCATCCT GCATCCTGTC 
ACCTCAAATA CAATCTTGGA AGGAAACCCA TTACTTCAAA TTGAAGTGGA ACCAACCTCC 
GAGAACGAAG AGAGCCACAA TGAAGCAGAG GAGTCCGAGG ACGAGTTTGA AGAGATGAAC 
CTATCCCTCC TCTCAGCCCG GAGCTTCCCA CGCAAAGCCA GCCAGACCAG CATCTTCCTT 
CAGGAGTGGG ACATCCCGTT TGAACAGCTA GAGATTGGCG AGCTCATCGG GAAGGGTCGC 
TTCGGGCAGG TTTACCACGG ACGCTGGCAC GGCGAGGTGG CCATCCGGTT GATTGACATA 
GAGAGGGACA ATGAGGACCA GCTTAAGGCC TTCAAGCGGG AAGTGATGGC CTACAGGCAG 
ACGAGGCACG AGAACGTGGT GCTCTTCATG GGAGCCTGCA TGAGCCCACC CCACTTGGCC 
ATCATCACCA GCCTCTGTAA GGGACGGACA CTCTACTCTG TGGTGAGGGA CGCCAAGATT 
GTCTTGGATG TCAACAAAAC CAGGCAGATC GCGCAAGAGA TTGTAAAGGG CATGGGCTAC 
CTCCACGCCA AGGGGATCCT TCATAAGGAC CTCAAATCGA AGAACGTCTT CTATGACAAC 
GGCAAAGTGG TCATCACAGA CTTTGGGCTC TTCAGCATTT CCGGGGTGCT GCAGGCTGGC 
CGGCGGGACG ACAAACTGCG GATCCAGAAC GGCTGGCTGT GTCACCTGGC CCCAGAAATC 
ATCCGCCAGC TGTCCCCTGA CACAGAAGAG GACAAGCTCC CATTCTCCAA GCACTCGGAT 
GTCTTTGCGC TTGGCACCAT CTGGTATGAG CTCCACGCCA GGGAGTGGCC ATTCAAGACC 
CAACCAGCAG AGGCAATCAT TTGGCAGATG GGCACGGGAA TGAAGCCCAA CCTCAGCCAG 
ATTGGCATGG GAAAAGAAAT CTCGGACATA CTGCTCTTCT GCTGGGCCTT CGAGCAGGAA 
GAGCGGCCCA CCTTTACCAA GCTCATGGAC ATGCTAGAGA AACTGCCAAA GCGAAACCGC 
CGCCTGTCGC ACCCCGGACA TTTCTGGAAG TCTGCAGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530457.1
CDS	706..2670
Translation	MICTLWRLVLSPSPKPPSESLHQAHHRGERIFGAKKKSKPLNLKIHSGVGSCENIPAQQRSPLLSERSLRSFFVGHGPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGDPARLVRTESVPCDINNPVRKPARYSDLHISQTLPKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCPRQKKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNTILEGNPLLQIEVEPTSENEESHNEAEESEDEFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRRDDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL

Target ORF information:

RefSeq Version	XM_006530394.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530394.3

ORF Insert Sequence:

ATGATCTGCA CCCTGTGGAG GTTGGTCCTC AGCCCTAGTC CCAAGCCTCC TTCAGAGAGT 
CTACACCAGG CCCACCACAG AGGCGAGAGA ATTTTTGGAG CCAAGAAGAA GAGCAAACCC 
TTGAACCTCA AGATCCACAG CGGCGTGGGC AGCTGCGAGA ACATCCCCGC CCAGCAACGC 
TCTCCGCTCC TGTCCGAGCG CTCCTTGCGG TCCTTCTTTG TGGGCCACGG GCCCTTCCTG 
CCCTCCACCC CACCTGTCCA CACTGAAGCC AATTTCTCTG CAAACACACT CTCTGTACCT 
CGCTGGTCAC CACAGATCCC ACGCAGAGAC CTTGGCAATT CCATCAAGCA CAGGTTTTCC 
ACCAAGTACT GGATGTCTCA GACGTGCACG GTCTGCGGGA AAGGGATGCT TTTTGGCCTC 
AAGTGTAAAA ACTGCAAGTT AAAGTGCCAC AATAAATGCA CCAAAGAAGC CCCGCCCTGT 
CACCTCCTGA TTATCCACCG AGGAGACCCA GCAAGGTTAG TGCGAACGGA GTCGGTGCCC 
TGTGACATCA ACAACCCGGT CCGGAAGCCT GCCCGATATT CAGATCTCCA CATCAGTCAG 
ACTCTCCCCA AAACCAACAA AATCAACAAG GACCACATCC CTGTCCCTTA CCAGCCAGAC 
TCCAGCAGCA ATCCCTCCTC CACGACGTCC TCCACGCCCT CCTCACCGGC ACCCCCCCTC 
CCTCCCAGCG CCACGCCGCC TTCTCCCCTG CACCCCTCCC CGCAGTGCCC GAGACAGAAA 
AAGAACTTCA ACCTGCCAGC TTCCCACTAC TACAAATACA AGCAACAGTT CATCTTCCCA 
GATGTGGTGC CTGTACCAGA GACACCCACC CGGGCACCCC AAGTCATCCT GCATCCTGTC 
ACCTCAAATA CAATCTTGGA AGGAAACCCA TTACTTCAAA TTGAAGTGGA ACCAACCTCC 
GAGAACGAAG AGAGCCACAA TGAAGCAGAG GAGTCCGAGG ACGAGTTTGA AGAGATGAAC 
CTATCCCTCC TCTCAGCCCG GAGCTTCCCA CGCAAAGCCA GCCAGACCAG CATCTTCCTT 
CAGGAGTGGG ACATCCCGTT TGAACAGCTA GAGATTGGCG AGCTCATCGG GAAGGGTCGC 
TTCGGGCAGG TTTACCACGG ACGCTGGCAC GGCGAGGTGG CCATCCGGTT GATTGACATA 
GAGAGGGACA ATGAGGACCA GCTTAAGGCC TTCAAGCGGG AAGTGATGGC CTACAGGCAG 
ACGAGGCACG AGAACGTGGT GCTCTTCATG GGAGCCTGCA TGAGCCCACC CCACTTGGCC 
ATCATCACCA GCCTCTGTAA GGGACGGACA CTCTACTCTG TGGTGAGGGA CGCCAAGATT 
GTCTTGGATG TCAACAAAAC CAGGCAGATC GCGCAAGAGA TTGTAAAGGG CATGGGCTAC 
CTCCACGCCA AGGGGATCCT TCATAAGGAC CTCAAATCGA AGAACGTCTT CTATGACAAC 
GGCAAAGTGG TCATCACAGA CTTTGGGCTC TTCAGCATTT CCGGGGTGCT GCAGGCTGGC 
CGGCGGGACG ACAAACTGCG GATCCAGAAC GGCTGGCTGT GTCACCTGGC CCCAGAAATC 
ATCCGCCAGC TGTCCCCTGA CACAGAAGAG GACAAGCTCC CATTCTCCAA GCACTCGGAT 
GTCTTTGCGC TTGGCACCAT CTGGTATGAG CTCCACGCCA GGGAGTGGCC ATTCAAGACC 
CAACCAGCAG AGGCAATCAT TTGGCAGATG GGCACGGGAA TGAAGCCCAA CCTCAGCCAG 
ATTGGCATGG GAAAAGAAAT CTCGGACATA CTGCTCTTCT GCTGGGCCTT CGAGCAGGAA 
GAGCGGCCCA CCTTTACCAA GCTCATGGAC ATGCTAGAGA AACTGCCAAA GCGAAACCGC 
CGCCTGTCGC ACCCCGGACA TTTCTGGAAG TCTGCAGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ksr2 ( XM_006530395.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530458.1 Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu35948
Clone ID Related Accession (Same CDS sequence)	XM_006530395.4 , XM_006530395.3
Accession Version	XM_006530395.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinase suppressor of Ras 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530395.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATA GGATGTTGCC GACCAACAGC AACAATGTAT CAGGCGCTTT GCCGAACACA 
CTCTCTGTAC CTCGCTGGTC ACCACAGATC CCACGCAGAG ACCTTGGCAA TTCCATCAAG 
CACAGGTTTT CCACCAAGTA CTGGATGTCT CAGACGTGCA CGGTCTGCGG GAAAGGGATG 
CTTTTTGGCC TCAAGTGTAA AAACTGCAAG TTAAAGTGCC ACAATAAATG CACCAAAGAA 
GCCCCGCCCT GTCACCTCCT GATTATCCAC CGAGGAGACC CAGCAAGGTT AGTGCGAACG 
GAGTCGGTGC CCTGTGACAT CAACAACCCG GTCCGGAAGC CTGCCCGATA TTCAGATCTC 
CACATCAGTC AGACTCTCCC CAAAACCAAC AAAATCAACA AGGACCACAT CCCTGTCCCT 
TACCAGCCAG ACTCCAGCAG CAATCCCTCC TCCACGACGT CCTCCACGCC CTCCTCACCG 
GCACCCCCCC TCCCTCCCAG CGCCACGCCG CCTTCTCCCC TGCACCCCTC CCCGCAGTGC 
CCGAGACAGA AAAAGAACTT CAACCTGCCA GCTTCCCACT ACTACAAATA CAAGCAACAG 
TTCATCTTCC CAGATGTGGT GCCTGTACCA GAGACACCCA CCCGGGCACC CCAAGTCATC 
CTGCATCCTG TCACCTCAAA TACAATCTTG GAAGGAAACC CATTACTTCA AATTGAAGTG 
GAACCAACCT CCGAGAACGA AGAGAGCCAC AATGAAGCAG AGGAGTCCGA GGACGAGTTT 
GAAGAGATGA ACCTATCCCT CCTCTCAGCC CGGAGCTTCC CACGCAAAGC CAGCCAGACC 
AGCATCTTCC TTCAGGAGTG GGACATCCCG TTTGAACAGC TAGAGATTGG CGAGCTCATC 
GGGAAGGGTC GCTTCGGGCA GGTTTACCAC GGACGCTGGC ACGGCGAGGT GGCCATCCGG 
TTGATTGACA TAGAGAGGGA CAATGAGGAC CAGCTTAAGG CCTTCAAGCG GGAAGTGATG 
GCCTACAGGC AGACGAGGCA CGAGAACGTG GTGCTCTTCA TGGGAGCCTG CATGAGCCCA 
CCCCACTTGG CCATCATCAC CAGCCTCTGT AAGGGACGGA CACTCTACTC TGTGGTGAGG 
GACGCCAAGA TTGTCTTGGA TGTCAACAAA ACCAGGCAGA TCGCGCAAGA GATTGTAAAG 
GGCATGGGCT ACCTCCACGC CAAGGGGATC CTTCATAAGG ACCTCAAATC GAAGAACGTC 
TTCTATGACA ACGGCAAAGT GGTCATCACA GACTTTGGGC TCTTCAGCAT TTCCGGGGTG 
CTGCAGGCTG GCCGGCGGGA CGACAAACTG CGGATCCAGA ACGGCTGGCT GTGTCACCTG 
GCCCCAGAAA TCATCCGCCA GCTGTCCCCT GACACAGAAG AGGACAAGCT CCCATTCTCC 
AAGCACTCGG ATGTCTTTGC GCTTGGCACC ATCTGGTATG AGCTCCACGC CAGGGAGTGG 
CCATTCAAGA CCCAACCAGC AGAGGCAATC ATTTGGCAGA TGGGCACGGG AATGAAGCCC 
AACCTCAGCC AGATTGGCAT GGGAAAAGAA ATCTCGGACA TACTGCTCTT CTGCTGGGCC 
TTCGAGCAGG AAGAGCGGCC CACCTTTACC AAGCTCATGG ACATGCTAGA GAAACTGCCA 
AAGCGAAACC GCCGCCTGTC GCACCCCGGA CATTTCTGGA AGTCTGCAGA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530458.1
CDS	576..2312
Translation	MMNRMLPTNSNNVSGALPNTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGDPARLVRTESVPCDINNPVRKPARYSDLHISQTLPKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCPRQKKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNTILEGNPLLQIEVEPTSENEESHNEAEESEDEFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRRDDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL

Target ORF information:

RefSeq Version	XM_006530395.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530395.3

ORF Insert Sequence:

ATGATGAATA GGATGTTGCC GACCAACAGC AACAATGTAT CAGGCGCTTT GCCGAACACA 
CTCTCTGTAC CTCGCTGGTC ACCACAGATC CCACGCAGAG ACCTTGGCAA TTCCATCAAG 
CACAGGTTTT CCACCAAGTA CTGGATGTCT CAGACGTGCA CGGTCTGCGG GAAAGGGATG 
CTTTTTGGCC TCAAGTGTAA AAACTGCAAG TTAAAGTGCC ACAATAAATG CACCAAAGAA 
GCCCCGCCCT GTCACCTCCT GATTATCCAC CGAGGAGACC CAGCAAGGTT AGTGCGAACG 
GAGTCGGTGC CCTGTGACAT CAACAACCCG GTCCGGAAGC CTGCCCGATA TTCAGATCTC 
CACATCAGTC AGACTCTCCC CAAAACCAAC AAAATCAACA AGGACCACAT CCCTGTCCCT 
TACCAGCCAG ACTCCAGCAG CAATCCCTCC TCCACGACGT CCTCCACGCC CTCCTCACCG 
GCACCCCCCC TCCCTCCCAG CGCCACGCCG CCTTCTCCCC TGCACCCCTC CCCGCAGTGC 
CCGAGACAGA AAAAGAACTT CAACCTGCCA GCTTCCCACT ACTACAAATA CAAGCAACAG 
TTCATCTTCC CAGATGTGGT GCCTGTACCA GAGACACCCA CCCGGGCACC CCAAGTCATC 
CTGCATCCTG TCACCTCAAA TACAATCTTG GAAGGAAACC CATTACTTCA AATTGAAGTG 
GAACCAACCT CCGAGAACGA AGAGAGCCAC AATGAAGCAG AGGAGTCCGA GGACGAGTTT 
GAAGAGATGA ACCTATCCCT CCTCTCAGCC CGGAGCTTCC CACGCAAAGC CAGCCAGACC 
AGCATCTTCC TTCAGGAGTG GGACATCCCG TTTGAACAGC TAGAGATTGG CGAGCTCATC 
GGGAAGGGTC GCTTCGGGCA GGTTTACCAC GGACGCTGGC ACGGCGAGGT GGCCATCCGG 
TTGATTGACA TAGAGAGGGA CAATGAGGAC CAGCTTAAGG CCTTCAAGCG GGAAGTGATG 
GCCTACAGGC AGACGAGGCA CGAGAACGTG GTGCTCTTCA TGGGAGCCTG CATGAGCCCA 
CCCCACTTGG CCATCATCAC CAGCCTCTGT AAGGGACGGA CACTCTACTC TGTGGTGAGG 
GACGCCAAGA TTGTCTTGGA TGTCAACAAA ACCAGGCAGA TCGCGCAAGA GATTGTAAAG 
GGCATGGGCT ACCTCCACGC CAAGGGGATC CTTCATAAGG ACCTCAAATC GAAGAACGTC 
TTCTATGACA ACGGCAAAGT GGTCATCACA GACTTTGGGC TCTTCAGCAT TTCCGGGGTG 
CTGCAGGCTG GCCGGCGGGA CGACAAACTG CGGATCCAGA ACGGCTGGCT GTGTCACCTG 
GCCCCAGAAA TCATCCGCCA GCTGTCCCCT GACACAGAAG AGGACAAGCT CCCATTCTCC 
AAGCACTCGG ATGTCTTTGC GCTTGGCACC ATCTGGTATG AGCTCCACGC CAGGGAGTGG 
CCATTCAAGA CCCAACCAGC AGAGGCAATC ATTTGGCAGA TGGGCACGGG AATGAAGCCC 
AACCTCAGCC AGATTGGCAT GGGAAAAGAA ATCTCGGACA TACTGCTCTT CTGCTGGGCC 
TTCGAGCAGG AAGAGCGGCC CACCTTTACC AAGCTCATGG ACATGCTAGA GAAACTGCCA 
AAGCGAAACC GCCGCCTGTC GCACCCCGGA CATTTCTGGA AGTCTGCAGA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ksr2 ( NM_001312914.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001299843.1 Mus musculus kinase suppressor of ras 2 (Ksr2), mRNA.

Price & Availability↑

CloneID	OMu74925
Clone ID Related Accession (Same CDS sequence)	NM_001312914.1
Accession Version	NM_001312914.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	kinase suppressor of Ras 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113299.14, AK137433.1, BC068233.1 and KJ719253.1. On Aug 23, 2015 this sequence version replaced XM_006530392.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGATGAGG AAAACATGAC GAAAAGCGAA GAGCAGCAAC CTCTGAGTTT GCAAAAAGCC 
TTGCAGCAGT GCGAGTTGGT TCAGAACATG ATCGACCTGA GCATCTCCAA TCTGGAAGGG 
CTTAGGACCA AGTGTGCAGC TTCCAACGAC CTCACGCAGA AGGAGATCAG GACCCTGGAG 
AGCAAGCTGG TGAAGTACTT CAGCCGACAG CTGTCCTGCA AGAAGAAGGT GGCTCTGCAG 
GAACGCAATG CTGAACTGGA CGGCTTCCCC CAGCTCCGCC ACTGGTTCCG GATCGTGGAT 
GTCCGAAAGG AAGTCCTGGA GGAGATCTCC CCTGACCAGC TGAGCTTGGA GGACCTCCTG 
GAGATGACCG ACGAGCAGGT GTGTGAGACC GTGGAGAAGT ATGGAGCCAA CCAGGAAGAG 
TGCGCCCGGC TCAACGCCTC ACTCTCCTGC CTCAGGAATG TGCACAAATC AGGAGGGAAT 
CTCTCCAAAC AAGATTGGAT CATCCAGTGG CCCACCACGG AGCCAGGGCA GGAGAGCAAC 
CCTGTGTGCC CCCCGGAGCC CAGCCCGTGG ATCCGAACCC ACCTCTCCCA GAGCCCCAGG 
GTCCAGACCA AGTGTCCCCA GCACTTCTGT CCTACCAGCC CTACGCCTGG GACCCCTGTG 
TACACCCAAG TGGACAGACT CACTGTAGAT GCCTACCCGA ACTTGTGCCC GCCGCCGCCA 
CCCCTGGAAT CAGGCCACCG CTCTCTGCCC CCGTCTCCCC GGCAGCGGCA CGTAGTCCGC 
ACCCCACCTC GCACCCCAAA TATTGTCACC ACCGTGACCC CACCTGGCAC GCCACCCATG 
AGGAGAAAAA ACAAGCTGAA GCCTCCCGGG ACGCCACCCC CCTCCTCTCG GAAGCTCATC 
CATCTGATCC CTGGCTTCAC AGCGCTGCAC AGGAGCAAAT CCCACGAGTT CCAGCTCGGG 
AACCGTGTAG ACGAGGCCAA CACACCCAAA GCCAAGAAGA AGAGCAAACC CTTGAACCTC 
AAGATCCACA GCGGCGTGGG CAGCTGCGAG AACATCCCCG CCCAGCAACG CTCTCCGCTC 
CTGTCCGAGC GCTCCTTGCG GTCCTTCTTT GTGGGCCACG GGCCCTTCCT GCCCTCCACC 
CCACCTGTCC ACACTGAAGC CAATTTCTCT GCAAACACAC TCTCTGTACC TCGCTGGTCA 
CCACAGATCC CACGCAGAGA CCTTGGCAAT TCCATCAAGC ACAGGTTTTC CACCAAGTAC 
TGGATGTCTC AGACGTGCAC GGTCTGCGGG AAAGGGATGC TTTTTGGCCT CAAGTGTAAA 
AACTGCAAGT TAAAGTGCCA CAATAAATGC ACCAAAGAAG CCCCGCCCTG TCACCTCCTG 
ATTATCCACC GAGGAGACCC AGCAAGGTTA GTGCGAACGG AGTCGGTGCC CTGTGACATC 
AACAACCCGG TCCGGAAGCC TGCCCGATAT TCAGATCTCC ACATCAGTCA GACTCTCCCC 
AAAACCAACA AAATCAACAA GGACCACATC CCTGTCCCTT ACCAGCCAGA CTCCAGCAGC 
AATCCCTCCT CCACGACGTC CTCCACGCCC TCCTCACCGG CACCCCCCCT CCCTCCCAGC 
GCCACGCCGC CTTCTCCCCT GCACCCCTCC CCGCAGTGCC CGAGACAGAA AAAGAACTTC 
AACCTGCCAG CTTCCCACTA CTACAAATAC AAGCAACAGT TCATCTTCCC AGATGTGGTG 
CCTGTACCAG AGACACCCAC CCGGGCACCC CAAGTCATCC TGCATCCTGT CACCTCAAAT 
ACAATCTTGG AAGGAAACCC ATTACTTCAA ATTGAAGTGG AACCAACCTC CGAGAACGAA 
GAGAGCCACA ATGAAGCAGA GGAGTCCGAG GACGAGTTTG AAGAGATGAA CCTATCCCTC 
CTCTCAGCCC GGAGCTTCCC ACGCAAAGCC AGCCAGACCA GCATCTTCCT TCAGGAGTGG 
GACATCCCGT TTGAACAGCT AGAGATTGGC GAGCTCATCG GGAAGGGTCG CTTCGGGCAG 
GTTTACCACG GACGCTGGCA CGGCGAGGTG GCCATCCGGT TGATTGACAT AGAGAGGGAC 
AATGAGGACC AGCTTAAGGC CTTCAAGCGG GAAGTGATGG CCTACAGGCA GACGAGGCAC 
GAGAACGTGG TGCTCTTCAT GGGAGCCTGC ATGAGCCCAC CCCACTTGGC CATCATCACC 
AGCCTCTGTA AGGGACGGAC ACTCTACTCT GTGGTGAGGG ACGCCAAGAT TGTCTTGGAT 
GTCAACAAAA CCAGGCAGAT CGCGCAAGAG ATTGTAAAGG GCATGGGCTA CCTCCACGCC 
AAGGGGATCC TTCATAAGGA CCTCAAATCG AAGAACGTCT TCTATGACAA CGGCAAAGTG 
GTCATCACAG ACTTTGGGCT CTTCAGCATT TCCGGGGTGC TGCAGGCTGG CCGGCGGGAC 
GACAAACTGC GGATCCAGAA CGGCTGGCTG TGTCACCTGG CCCCAGAAAT CATCCGCCAG 
CTGTCCCCTG ACACAGAAGA GGACAAGCTC CCATTCTCCA AGCACTCGGA TGTCTTTGCG 
CTTGGCACCA TCTGGTATGA GCTCCACGCC AGGGAGTGGC CATTCAAGAC CCAACCAGCA 
GAGGCAATCA TTTGGCAGAT GGGCACGGGA ATGAAGCCCA ACCTCAGCCA GATTGGCATG 
GGAAAAGAAA TCTCGGACAT ACTGCTCTTC TGCTGGGCCT TCGAGCAGGA AGAGCGGCCC 
ACCTTTACCA AGCTCATGGA CATGCTAGAG AAACTGCCAA AGCGAAACCG CCGCCTGTCG 
CACCCCGGAC ATTTCTGGAA GTCTGCAGAG CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299843.1
CDS	828..3683
Translation	MDEENMTKSEEQQPLSLQKALQQCELVQNMIDLSISNLEGLRTKCAASNDLTQKEIRTLESKLVKYFSRQLSCKKKVALQERNAELDGFPQLRHWFRIVDVRKEVLEEISPDQLSLEDLLEMTDEQVCETVEKYGANQEECARLNASLSCLRNVHKSGGNLSKQDWIIQWPTTEPGQESNPVCPPEPSPWIRTHLSQSPRVQTKCPQHFCPTSPTPGTPVYTQVDRLTVDAYPNLCPPPPPLESGHRSLPPSPRQRHVVRTPPRTPNIVTTVTPPGTPPMRRKNKLKPPGTPPPSSRKLIHLIPGFTALHRSKSHEFQLGNRVDEANTPKAKKKSKPLNLKIHSGVGSCENIPAQQRSPLLSERSLRSFFVGHGPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGDPARLVRTESVPCDINNPVRKPARYSDLHISQTLPKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCPRQKKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNTILEGNPLLQIEVEPTSENEESHNEAEESEDEFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRRDDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL

Target ORF information:

RefSeq Version	NM_001312914.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinase suppressor of ras 2 (Ksr2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312914.1

ORF Insert Sequence:

ATGGATGAGG AAAACATGAC GAAAAGCGAA GAGCAGCAAC CTCTGAGTTT GCAAAAAGCC 
TTGCAGCAGT GCGAGTTGGT TCAGAACATG ATCGACCTGA GCATCTCCAA TCTGGAAGGG 
CTTAGGACCA AGTGTGCAGC TTCCAACGAC CTCACGCAGA AGGAGATCAG GACCCTGGAG 
AGCAAGCTGG TGAAGTACTT CAGCCGACAG CTGTCCTGCA AGAAGAAGGT GGCTCTGCAG 
GAACGCAATG CTGAACTGGA CGGCTTCCCC CAGCTCCGCC ACTGGTTCCG GATCGTGGAT 
GTCCGAAAGG AAGTCCTGGA GGAGATCTCC CCTGACCAGC TGAGCTTGGA GGACCTCCTG 
GAGATGACCG ACGAGCAGGT GTGTGAGACC GTGGAGAAGT ATGGAGCCAA CCAGGAAGAG 
TGCGCCCGGC TCAACGCCTC ACTCTCCTGC CTCAGGAATG TGCACAAATC AGGAGGGAAT 
CTCTCCAAAC AAGATTGGAT CATCCAGTGG CCCACCACGG AGCCAGGGCA GGAGAGCAAC 
CCTGTGTGCC CCCCGGAGCC CAGCCCGTGG ATCCGAACCC ACCTCTCCCA GAGCCCCAGG 
GTCCAGACCA AGTGTCCCCA GCACTTCTGT CCTACCAGCC CTACGCCTGG GACCCCTGTG 
TACACCCAAG TGGACAGACT CACTGTAGAT GCCTACCCGA ACTTGTGCCC GCCGCCGCCA 
CCCCTGGAAT CAGGCCACCG CTCTCTGCCC CCGTCTCCCC GGCAGCGGCA CGTAGTCCGC 
ACCCCACCTC GCACCCCAAA TATTGTCACC ACCGTGACCC CACCTGGCAC GCCACCCATG 
AGGAGAAAAA ACAAGCTGAA GCCTCCCGGG ACGCCACCCC CCTCCTCTCG GAAGCTCATC 
CATCTGATCC CTGGCTTCAC AGCGCTGCAC AGGAGCAAAT CCCACGAGTT CCAGCTCGGG 
AACCGTGTAG ACGAGGCCAA CACACCCAAA GCCAAGAAGA AGAGCAAACC CTTGAACCTC 
AAGATCCACA GCGGCGTGGG CAGCTGCGAG AACATCCCCG CCCAGCAACG CTCTCCGCTC 
CTGTCCGAGC GCTCCTTGCG GTCCTTCTTT GTGGGCCACG GGCCCTTCCT GCCCTCCACC 
CCACCTGTCC ACACTGAAGC CAATTTCTCT GCAAACACAC TCTCTGTACC TCGCTGGTCA 
CCACAGATCC CACGCAGAGA CCTTGGCAAT TCCATCAAGC ACAGGTTTTC CACCAAGTAC 
TGGATGTCTC AGACGTGCAC GGTCTGCGGG AAAGGGATGC TTTTTGGCCT CAAGTGTAAA 
AACTGCAAGT TAAAGTGCCA CAATAAATGC ACCAAAGAAG CCCCGCCCTG TCACCTCCTG 
ATTATCCACC GAGGAGACCC AGCAAGGTTA GTGCGAACGG AGTCGGTGCC CTGTGACATC 
AACAACCCGG TCCGGAAGCC TGCCCGATAT TCAGATCTCC ACATCAGTCA GACTCTCCCC 
AAAACCAACA AAATCAACAA GGACCACATC CCTGTCCCTT ACCAGCCAGA CTCCAGCAGC 
AATCCCTCCT CCACGACGTC CTCCACGCCC TCCTCACCGG CACCCCCCCT CCCTCCCAGC 
GCCACGCCGC CTTCTCCCCT GCACCCCTCC CCGCAGTGCC CGAGACAGAA AAAGAACTTC 
AACCTGCCAG CTTCCCACTA CTACAAATAC AAGCAACAGT TCATCTTCCC AGATGTGGTG 
CCTGTACCAG AGACACCCAC CCGGGCACCC CAAGTCATCC TGCATCCTGT CACCTCAAAT 
ACAATCTTGG AAGGAAACCC ATTACTTCAA ATTGAAGTGG AACCAACCTC CGAGAACGAA 
GAGAGCCACA ATGAAGCAGA GGAGTCCGAG GACGAGTTTG AAGAGATGAA CCTATCCCTC 
CTCTCAGCCC GGAGCTTCCC ACGCAAAGCC AGCCAGACCA GCATCTTCCT TCAGGAGTGG 
GACATCCCGT TTGAACAGCT AGAGATTGGC GAGCTCATCG GGAAGGGTCG CTTCGGGCAG 
GTTTACCACG GACGCTGGCA CGGCGAGGTG GCCATCCGGT TGATTGACAT AGAGAGGGAC 
AATGAGGACC AGCTTAAGGC CTTCAAGCGG GAAGTGATGG CCTACAGGCA GACGAGGCAC 
GAGAACGTGG TGCTCTTCAT GGGAGCCTGC ATGAGCCCAC CCCACTTGGC CATCATCACC 
AGCCTCTGTA AGGGACGGAC ACTCTACTCT GTGGTGAGGG ACGCCAAGAT TGTCTTGGAT 
GTCAACAAAA CCAGGCAGAT CGCGCAAGAG ATTGTAAAGG GCATGGGCTA CCTCCACGCC 
AAGGGGATCC TTCATAAGGA CCTCAAATCG AAGAACGTCT TCTATGACAA CGGCAAAGTG 
GTCATCACAG ACTTTGGGCT CTTCAGCATT TCCGGGGTGC TGCAGGCTGG CCGGCGGGAC 
GACAAACTGC GGATCCAGAA CGGCTGGCTG TGTCACCTGG CCCCAGAAAT CATCCGCCAG 
CTGTCCCCTG ACACAGAAGA GGACAAGCTC CCATTCTCCA AGCACTCGGA TGTCTTTGCG 
CTTGGCACCA TCTGGTATGA GCTCCACGCC AGGGAGTGGC CATTCAAGAC CCAACCAGCA 
GAGGCAATCA TTTGGCAGAT GGGCACGGGA ATGAAGCCCA ACCTCAGCCA GATTGGCATG 
GGAAAAGAAA TCTCGGACAT ACTGCTCTTC TGCTGGGCCT TCGAGCAGGA AGAGCGGCCC 
ACCTTTACCA AGCTCATGGA CATGCTAGAG AAACTGCCAA AGCGAAACCG CCGCCTGTCG 
CACCCCGGAC ATTTCTGGAA GTCTGCAGAG CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ksr2 ( XM_006530393.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530456.1 Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X1, mRNA.

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CloneID	OMu35946
Clone ID Related Accession (Same CDS sequence)	XM_006530393.3 , XM_006530393.4
Accession Version	XM_006530393.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinase suppressor of Ras 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530393.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAAACATGAC GAAAAGCGAA GAGCAGCAAC CTCTGAGTTT GCAAAAAGCC 
TTGCAGCAGT GCGAGTTGGT TCAGAACATG ATCGACCTGA GCATCTCCAA TCTGGAAGGG 
CTTAGGACCA AGTGTGCAGC TTCCAACGAC CTCACGCAGA AGGAGATCAG GACCCTGGAG 
AGCAAGCTGG TGAAGTACTT CAGCCGACAG CTGTCCTGCA AGAAGAAGGT GGCTCTGCAG 
GAACGCAATG CTGAACTGGA CGGCTTCCCC CAGCTCCGCC ACTGGTTCCG GATCGTGGAT 
GTCCGAAAGG AAGTCCTGGA GGAGATCTCC CCTGACCAGC TGAGCTTGGA GGACCTCCTG 
GAGATGACCG ACGAGCAGGT GTGTGAGACC GTGGAGAAGT ATGGAGCCAA CCAGGAAGAG 
TGCGCCCGGC TCAACGCCTC ACTCTCCTGC CTCAGGAATG TGCACAAATC AGGAGGGAAT 
CTCTCCAAAC AAGATTGGAT CATCCAGTGG CCCACCACGG AGCCAGGGCA GGAGAGCAAC 
CCTGTGTGCC CCCCGGAGCC CAGCCCGTGG ATCCGAACCC ACCTCTCCCA GAGCCCCAGG 
GTCCAGACCA AGTGTCCCCA GCACTTCTGT CCTACCAGCC CTACGCCTGG GACCCCTGTG 
TACACCCAAG TGGACAGACT CACTGTAGAT GCCTACCCGA ACTTGTGCCC GCCGCCGCCA 
CCCCTGGAAT CAGGCCACCG CTCTCTGCCC CCGTCTCCCC GGCAGCGGCA CGTAGTCCGC 
ACCCCACCTC GCACCCCAAA TATTGTCACC ACCGTGACCC CACCTGGCAC GCCACCCATG 
AGGAGAAAAA ACAAGCTGAA GCCTCCCGGG ACGCCACCCC CCTCCTCTCG GAAGCTCATC 
CATCTGATCC CTGGCTTCAC AGCGCTGCAC AGGAGCAAAT CCCACGAGTT CCAGCTCGGG 
AACCGTGTAG ACGAGGCCAA CACACCCAAA GCCAAGAAGA AGAGCAAACC CTTGAACCTC 
AAGATCCACA GCGGCGTGGG CAGCTGCGAG AACATCCCCG CCCAGCAACG CTCTCCGCTC 
CTGTCCGAGC GCTCCTTGCG GTCCTTCTTT GTGGGCCACG GGCCCTTCCT GCCCTCCACC 
CCACCTGTCC ACACTGAAGC CAATTTCTCT GCAAACACAC TCTCTGTACC TCGCTGGTCA 
CCACAGATCC CACGCAGAGA CCTTGGCAAT TCCATCAAGC ACAGGTTTTC CACCAAGTAC 
TGGATGTCTC AGACGTGCAC GGTCTGCGGG AAAGGGATGC TTTTTGGCCT CAAGTGTAAA 
AACTGCAAGT TAAAGTGCCA CAATAAATGC ACCAAAGAAG CCCCGCCCTG TCACCTCCTG 
ATTATCCACC GAGGAGCAAG GTTAGTGCGA ACGGAGTCGG TGCCCTGTGA CATCAACAAC 
CCGGTCCGGA AGCCTGCCCG ATATTCAGAT CTCCACATCA GTCAGACTCT CCCCAAAACC 
AACAAAATCA ACAAGGACCA CATCCCTGTC CCTTACCAGC CAGACTCCAG CAGCAATCCC 
TCCTCCACGA CGTCCTCCAC GCCCTCCTCA CCGGCACCCC CCCTCCCTCC CAGCGCCACG 
CCGCCTTCTC CCCTGCACCC CTCCCCGCAG TGCCCGAGAC AGAAAAAGAA CTTCAACCTG 
CCAGCTTCCC ACTACTACAA ATACAAGCAA CAGTTCATCT TCCCAGATGT GGTGCCTGTA 
CCAGAGACAC CCACCCGGGC ACCCCAAGTC ATCCTGCATC CTGTCACCTC AAATACAATC 
TTGGAAGGAA ACCCATTACT TCAAATTGAA GTGGAACCAA CCTCCGAGAA CGAAGAGAGC 
CACAATGAAG CAGAGGAGTC CGAGGACGAG TTTGAAGAGA TGAACCTATC CCTCCTCTCA 
GCCCGGAGCT TCCCACGCAA AGCCAGCCAG ACCAGCATCT TCCTTCAGGA GTGGGACATC 
CCGTTTGAAC AGCTAGAGAT TGGCGAGCTC ATCGGGAAGG GTCGCTTCGG GCAGGTTTAC 
CACGGACGCT GGCACGGCGA GGTGGCCATC CGGTTGATTG ACATAGAGAG GGACAATGAG 
GACCAGCTTA AGGCCTTCAA GCGGGAAGTG ATGGCCTACA GGCAGACGAG GCACGAGAAC 
GTGGTGCTCT TCATGGGAGC CTGCATGAGC CCACCCCACT TGGCCATCAT CACCAGCCTC 
TGTAAGGGAC GGACACTCTA CTCTGTGGTG AGGGACGCCA AGATTGTCTT GGATGTCAAC 
AAAACCAGGC AGATCGCGCA AGAGATTGTA AAGGGCATGG GCTACCTCCA CGCCAAGGGG 
ATCCTTCATA AGGACCTCAA ATCGAAGAAC GTCTTCTATG ACAACGGCAA AGTGGTCATC 
ACAGACTTTG GGCTCTTCAG CATTTCCGGG GTGCTGCAGG CTGGCCGGCG GGACGACAAA 
CTGCGGATCC AGAACGGCTG GCTGTGTCAC CTGGCCCCAG AAATCATCCG CCAGCTGTCC 
CCTGACACAG AAGAGGACAA GCTCCCATTC TCCAAGCACT CGGATGTCTT TGCGCTTGGC 
ACCATCTGGT ATGAGCTCCA CGCCAGGGAG TGGCCATTCA AGACCCAACC AGCAGAGGCA 
ATCATTTGGC AGATGGGCAC GGGAATGAAG CCCAACCTCA GCCAGATTGG CATGGGAAAA 
GAAATCTCGG ACATACTGCT CTTCTGCTGG GCCTTCGAGC AGGAAGAGCG GCCCACCTTT 
ACCAAGCTCA TGGACATGCT AGAGAAACTG CCAAAGCGAA ACCGCCGCCT GTCGCACCCC 
GGACATTTCT GGAAGTCTGC AGAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530456.1
CDS	1609..4458
Translation	MDEENMTKSEEQQPLSLQKALQQCELVQNMIDLSISNLEGLRTKCAASNDLTQKEIRTLESKLVKYFSRQLSCKKKVALQERNAELDGFPQLRHWFRIVDVRKEVLEEISPDQLSLEDLLEMTDEQVCETVEKYGANQEECARLNASLSCLRNVHKSGGNLSKQDWIIQWPTTEPGQESNPVCPPEPSPWIRTHLSQSPRVQTKCPQHFCPTSPTPGTPVYTQVDRLTVDAYPNLCPPPPPLESGHRSLPPSPRQRHVVRTPPRTPNIVTTVTPPGTPPMRRKNKLKPPGTPPPSSRKLIHLIPGFTALHRSKSHEFQLGNRVDEANTPKAKKKSKPLNLKIHSGVGSCENIPAQQRSPLLSERSLRSFFVGHGPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGARLVRTESVPCDINNPVRKPARYSDLHISQTLPKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCPRQKKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNTILEGNPLLQIEVEPTSENEESHNEAEESEDEFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRRDDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL

Target ORF information:

RefSeq Version	XM_006530393.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530393.3

ORF Insert Sequence:

ATGGATGAGG AAAACATGAC GAAAAGCGAA GAGCAGCAAC CTCTGAGTTT GCAAAAAGCC 
TTGCAGCAGT GCGAGTTGGT TCAGAACATG ATCGACCTGA GCATCTCCAA TCTGGAAGGG 
CTTAGGACCA AGTGTGCAGC TTCCAACGAC CTCACGCAGA AGGAGATCAG GACCCTGGAG 
AGCAAGCTGG TGAAGTACTT CAGCCGACAG CTGTCCTGCA AGAAGAAGGT GGCTCTGCAG 
GAACGCAATG CTGAACTGGA CGGCTTCCCC CAGCTCCGCC ACTGGTTCCG GATCGTGGAT 
GTCCGAAAGG AAGTCCTGGA GGAGATCTCC CCTGACCAGC TGAGCTTGGA GGACCTCCTG 
GAGATGACCG ACGAGCAGGT GTGTGAGACC GTGGAGAAGT ATGGAGCCAA CCAGGAAGAG 
TGCGCCCGGC TCAACGCCTC ACTCTCCTGC CTCAGGAATG TGCACAAATC AGGAGGGAAT 
CTCTCCAAAC AAGATTGGAT CATCCAGTGG CCCACCACGG AGCCAGGGCA GGAGAGCAAC 
CCTGTGTGCC CCCCGGAGCC CAGCCCGTGG ATCCGAACCC ACCTCTCCCA GAGCCCCAGG 
GTCCAGACCA AGTGTCCCCA GCACTTCTGT CCTACCAGCC CTACGCCTGG GACCCCTGTG 
TACACCCAAG TGGACAGACT CACTGTAGAT GCCTACCCGA ACTTGTGCCC GCCGCCGCCA 
CCCCTGGAAT CAGGCCACCG CTCTCTGCCC CCGTCTCCCC GGCAGCGGCA CGTAGTCCGC 
ACCCCACCTC GCACCCCAAA TATTGTCACC ACCGTGACCC CACCTGGCAC GCCACCCATG 
AGGAGAAAAA ACAAGCTGAA GCCTCCCGGG ACGCCACCCC CCTCCTCTCG GAAGCTCATC 
CATCTGATCC CTGGCTTCAC AGCGCTGCAC AGGAGCAAAT CCCACGAGTT CCAGCTCGGG 
AACCGTGTAG ACGAGGCCAA CACACCCAAA GCCAAGAAGA AGAGCAAACC CTTGAACCTC 
AAGATCCACA GCGGCGTGGG CAGCTGCGAG AACATCCCCG CCCAGCAACG CTCTCCGCTC 
CTGTCCGAGC GCTCCTTGCG GTCCTTCTTT GTGGGCCACG GGCCCTTCCT GCCCTCCACC 
CCACCTGTCC ACACTGAAGC CAATTTCTCT GCAAACACAC TCTCTGTACC TCGCTGGTCA 
CCACAGATCC CACGCAGAGA CCTTGGCAAT TCCATCAAGC ACAGGTTTTC CACCAAGTAC 
TGGATGTCTC AGACGTGCAC GGTCTGCGGG AAAGGGATGC TTTTTGGCCT CAAGTGTAAA 
AACTGCAAGT TAAAGTGCCA CAATAAATGC ACCAAAGAAG CCCCGCCCTG TCACCTCCTG 
ATTATCCACC GAGGAGCAAG GTTAGTGCGA ACGGAGTCGG TGCCCTGTGA CATCAACAAC 
CCGGTCCGGA AGCCTGCCCG ATATTCAGAT CTCCACATCA GTCAGACTCT CCCCAAAACC 
AACAAAATCA ACAAGGACCA CATCCCTGTC CCTTACCAGC CAGACTCCAG CAGCAATCCC 
TCCTCCACGA CGTCCTCCAC GCCCTCCTCA CCGGCACCCC CCCTCCCTCC CAGCGCCACG 
CCGCCTTCTC CCCTGCACCC CTCCCCGCAG TGCCCGAGAC AGAAAAAGAA CTTCAACCTG 
CCAGCTTCCC ACTACTACAA ATACAAGCAA CAGTTCATCT TCCCAGATGT GGTGCCTGTA 
CCAGAGACAC CCACCCGGGC ACCCCAAGTC ATCCTGCATC CTGTCACCTC AAATACAATC 
TTGGAAGGAA ACCCATTACT TCAAATTGAA GTGGAACCAA CCTCCGAGAA CGAAGAGAGC 
CACAATGAAG CAGAGGAGTC CGAGGACGAG TTTGAAGAGA TGAACCTATC CCTCCTCTCA 
GCCCGGAGCT TCCCACGCAA AGCCAGCCAG ACCAGCATCT TCCTTCAGGA GTGGGACATC 
CCGTTTGAAC AGCTAGAGAT TGGCGAGCTC ATCGGGAAGG GTCGCTTCGG GCAGGTTTAC 
CACGGACGCT GGCACGGCGA GGTGGCCATC CGGTTGATTG ACATAGAGAG GGACAATGAG 
GACCAGCTTA AGGCCTTCAA GCGGGAAGTG ATGGCCTACA GGCAGACGAG GCACGAGAAC 
GTGGTGCTCT TCATGGGAGC CTGCATGAGC CCACCCCACT TGGCCATCAT CACCAGCCTC 
TGTAAGGGAC GGACACTCTA CTCTGTGGTG AGGGACGCCA AGATTGTCTT GGATGTCAAC 
AAAACCAGGC AGATCGCGCA AGAGATTGTA AAGGGCATGG GCTACCTCCA CGCCAAGGGG 
ATCCTTCATA AGGACCTCAA ATCGAAGAAC GTCTTCTATG ACAACGGCAA AGTGGTCATC 
ACAGACTTTG GGCTCTTCAG CATTTCCGGG GTGCTGCAGG CTGGCCGGCG GGACGACAAA 
CTGCGGATCC AGAACGGCTG GCTGTGTCAC CTGGCCCCAG AAATCATCCG CCAGCTGTCC 
CCTGACACAG AAGAGGACAA GCTCCCATTC TCCAAGCACT CGGATGTCTT TGCGCTTGGC 
ACCATCTGGT ATGAGCTCCA CGCCAGGGAG TGGCCATTCA AGACCCAACC AGCAGAGGCA 
ATCATTTGGC AGATGGGCAC GGGAATGAAG CCCAACCTCA GCCAGATTGG CATGGGAAAA 
GAAATCTCGG ACATACTGCT CTTCTGCTGG GCCTTCGAGC AGGAAGAGCG GCCCACCTTT 
ACCAAGCTCA TGGACATGCT AGAGAAACTG CCAAAGCGAA ACCGCCGCCT GTCGCACCCC 
GGACATTTCT GGAAGTCTGC AGAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ksr2 ( XM_006530393.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530456.1 Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35946
Clone ID Related Accession (Same CDS sequence)	XM_006530393.3 , XM_006530393.4
Accession Version	XM_006530393.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinase suppressor of Ras 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530393.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAAACATGAC GAAAAGCGAA GAGCAGCAAC CTCTGAGTTT GCAAAAAGCC 
TTGCAGCAGT GCGAGTTGGT TCAGAACATG ATCGACCTGA GCATCTCCAA TCTGGAAGGG 
CTTAGGACCA AGTGTGCAGC TTCCAACGAC CTCACGCAGA AGGAGATCAG GACCCTGGAG 
AGCAAGCTGG TGAAGTACTT CAGCCGACAG CTGTCCTGCA AGAAGAAGGT GGCTCTGCAG 
GAACGCAATG CTGAACTGGA CGGCTTCCCC CAGCTCCGCC ACTGGTTCCG GATCGTGGAT 
GTCCGAAAGG AAGTCCTGGA GGAGATCTCC CCTGACCAGC TGAGCTTGGA GGACCTCCTG 
GAGATGACCG ACGAGCAGGT GTGTGAGACC GTGGAGAAGT ATGGAGCCAA CCAGGAAGAG 
TGCGCCCGGC TCAACGCCTC ACTCTCCTGC CTCAGGAATG TGCACAAATC AGGAGGGAAT 
CTCTCCAAAC AAGATTGGAT CATCCAGTGG CCCACCACGG AGCCAGGGCA GGAGAGCAAC 
CCTGTGTGCC CCCCGGAGCC CAGCCCGTGG ATCCGAACCC ACCTCTCCCA GAGCCCCAGG 
GTCCAGACCA AGTGTCCCCA GCACTTCTGT CCTACCAGCC CTACGCCTGG GACCCCTGTG 
TACACCCAAG TGGACAGACT CACTGTAGAT GCCTACCCGA ACTTGTGCCC GCCGCCGCCA 
CCCCTGGAAT CAGGCCACCG CTCTCTGCCC CCGTCTCCCC GGCAGCGGCA CGTAGTCCGC 
ACCCCACCTC GCACCCCAAA TATTGTCACC ACCGTGACCC CACCTGGCAC GCCACCCATG 
AGGAGAAAAA ACAAGCTGAA GCCTCCCGGG ACGCCACCCC CCTCCTCTCG GAAGCTCATC 
CATCTGATCC CTGGCTTCAC AGCGCTGCAC AGGAGCAAAT CCCACGAGTT CCAGCTCGGG 
AACCGTGTAG ACGAGGCCAA CACACCCAAA GCCAAGAAGA AGAGCAAACC CTTGAACCTC 
AAGATCCACA GCGGCGTGGG CAGCTGCGAG AACATCCCCG CCCAGCAACG CTCTCCGCTC 
CTGTCCGAGC GCTCCTTGCG GTCCTTCTTT GTGGGCCACG GGCCCTTCCT GCCCTCCACC 
CCACCTGTCC ACACTGAAGC CAATTTCTCT GCAAACACAC TCTCTGTACC TCGCTGGTCA 
CCACAGATCC CACGCAGAGA CCTTGGCAAT TCCATCAAGC ACAGGTTTTC CACCAAGTAC 
TGGATGTCTC AGACGTGCAC GGTCTGCGGG AAAGGGATGC TTTTTGGCCT CAAGTGTAAA 
AACTGCAAGT TAAAGTGCCA CAATAAATGC ACCAAAGAAG CCCCGCCCTG TCACCTCCTG 
ATTATCCACC GAGGAGCAAG GTTAGTGCGA ACGGAGTCGG TGCCCTGTGA CATCAACAAC 
CCGGTCCGGA AGCCTGCCCG ATATTCAGAT CTCCACATCA GTCAGACTCT CCCCAAAACC 
AACAAAATCA ACAAGGACCA CATCCCTGTC CCTTACCAGC CAGACTCCAG CAGCAATCCC 
TCCTCCACGA CGTCCTCCAC GCCCTCCTCA CCGGCACCCC CCCTCCCTCC CAGCGCCACG 
CCGCCTTCTC CCCTGCACCC CTCCCCGCAG TGCCCGAGAC AGAAAAAGAA CTTCAACCTG 
CCAGCTTCCC ACTACTACAA ATACAAGCAA CAGTTCATCT TCCCAGATGT GGTGCCTGTA 
CCAGAGACAC CCACCCGGGC ACCCCAAGTC ATCCTGCATC CTGTCACCTC AAATACAATC 
TTGGAAGGAA ACCCATTACT TCAAATTGAA GTGGAACCAA CCTCCGAGAA CGAAGAGAGC 
CACAATGAAG CAGAGGAGTC CGAGGACGAG TTTGAAGAGA TGAACCTATC CCTCCTCTCA 
GCCCGGAGCT TCCCACGCAA AGCCAGCCAG ACCAGCATCT TCCTTCAGGA GTGGGACATC 
CCGTTTGAAC AGCTAGAGAT TGGCGAGCTC ATCGGGAAGG GTCGCTTCGG GCAGGTTTAC 
CACGGACGCT GGCACGGCGA GGTGGCCATC CGGTTGATTG ACATAGAGAG GGACAATGAG 
GACCAGCTTA AGGCCTTCAA GCGGGAAGTG ATGGCCTACA GGCAGACGAG GCACGAGAAC 
GTGGTGCTCT TCATGGGAGC CTGCATGAGC CCACCCCACT TGGCCATCAT CACCAGCCTC 
TGTAAGGGAC GGACACTCTA CTCTGTGGTG AGGGACGCCA AGATTGTCTT GGATGTCAAC 
AAAACCAGGC AGATCGCGCA AGAGATTGTA AAGGGCATGG GCTACCTCCA CGCCAAGGGG 
ATCCTTCATA AGGACCTCAA ATCGAAGAAC GTCTTCTATG ACAACGGCAA AGTGGTCATC 
ACAGACTTTG GGCTCTTCAG CATTTCCGGG GTGCTGCAGG CTGGCCGGCG GGACGACAAA 
CTGCGGATCC AGAACGGCTG GCTGTGTCAC CTGGCCCCAG AAATCATCCG CCAGCTGTCC 
CCTGACACAG AAGAGGACAA GCTCCCATTC TCCAAGCACT CGGATGTCTT TGCGCTTGGC 
ACCATCTGGT ATGAGCTCCA CGCCAGGGAG TGGCCATTCA AGACCCAACC AGCAGAGGCA 
ATCATTTGGC AGATGGGCAC GGGAATGAAG CCCAACCTCA GCCAGATTGG CATGGGAAAA 
GAAATCTCGG ACATACTGCT CTTCTGCTGG GCCTTCGAGC AGGAAGAGCG GCCCACCTTT 
ACCAAGCTCA TGGACATGCT AGAGAAACTG CCAAAGCGAA ACCGCCGCCT GTCGCACCCC 
GGACATTTCT GGAAGTCTGC AGAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530456.1
CDS	184..3033
Translation	MDEENMTKSEEQQPLSLQKALQQCELVQNMIDLSISNLEGLRTKCAASNDLTQKEIRTLESKLVKYFSRQLSCKKKVALQERNAELDGFPQLRHWFRIVDVRKEVLEEISPDQLSLEDLLEMTDEQVCETVEKYGANQEECARLNASLSCLRNVHKSGGNLSKQDWIIQWPTTEPGQESNPVCPPEPSPWIRTHLSQSPRVQTKCPQHFCPTSPTPGTPVYTQVDRLTVDAYPNLCPPPPPLESGHRSLPPSPRQRHVVRTPPRTPNIVTTVTPPGTPPMRRKNKLKPPGTPPPSSRKLIHLIPGFTALHRSKSHEFQLGNRVDEANTPKAKKKSKPLNLKIHSGVGSCENIPAQQRSPLLSERSLRSFFVGHGPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGARLVRTESVPCDINNPVRKPARYSDLHISQTLPKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCPRQKKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNTILEGNPLLQIEVEPTSENEESHNEAEESEDEFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRRDDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL

Target ORF information:

RefSeq Version	XM_006530393.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530393.4

ORF Insert Sequence:

ATGGATGAGG AAAACATGAC GAAAAGCGAA GAGCAGCAAC CTCTGAGTTT GCAAAAAGCC 
TTGCAGCAGT GCGAGTTGGT TCAGAACATG ATCGACCTGA GCATCTCCAA TCTGGAAGGG 
CTTAGGACCA AGTGTGCAGC TTCCAACGAC CTCACGCAGA AGGAGATCAG GACCCTGGAG 
AGCAAGCTGG TGAAGTACTT CAGCCGACAG CTGTCCTGCA AGAAGAAGGT GGCTCTGCAG 
GAACGCAATG CTGAACTGGA CGGCTTCCCC CAGCTCCGCC ACTGGTTCCG GATCGTGGAT 
GTCCGAAAGG AAGTCCTGGA GGAGATCTCC CCTGACCAGC TGAGCTTGGA GGACCTCCTG 
GAGATGACCG ACGAGCAGGT GTGTGAGACC GTGGAGAAGT ATGGAGCCAA CCAGGAAGAG 
TGCGCCCGGC TCAACGCCTC ACTCTCCTGC CTCAGGAATG TGCACAAATC AGGAGGGAAT 
CTCTCCAAAC AAGATTGGAT CATCCAGTGG CCCACCACGG AGCCAGGGCA GGAGAGCAAC 
CCTGTGTGCC CCCCGGAGCC CAGCCCGTGG ATCCGAACCC ACCTCTCCCA GAGCCCCAGG 
GTCCAGACCA AGTGTCCCCA GCACTTCTGT CCTACCAGCC CTACGCCTGG GACCCCTGTG 
TACACCCAAG TGGACAGACT CACTGTAGAT GCCTACCCGA ACTTGTGCCC GCCGCCGCCA 
CCCCTGGAAT CAGGCCACCG CTCTCTGCCC CCGTCTCCCC GGCAGCGGCA CGTAGTCCGC 
ACCCCACCTC GCACCCCAAA TATTGTCACC ACCGTGACCC CACCTGGCAC GCCACCCATG 
AGGAGAAAAA ACAAGCTGAA GCCTCCCGGG ACGCCACCCC CCTCCTCTCG GAAGCTCATC 
CATCTGATCC CTGGCTTCAC AGCGCTGCAC AGGAGCAAAT CCCACGAGTT CCAGCTCGGG 
AACCGTGTAG ACGAGGCCAA CACACCCAAA GCCAAGAAGA AGAGCAAACC CTTGAACCTC 
AAGATCCACA GCGGCGTGGG CAGCTGCGAG AACATCCCCG CCCAGCAACG CTCTCCGCTC 
CTGTCCGAGC GCTCCTTGCG GTCCTTCTTT GTGGGCCACG GGCCCTTCCT GCCCTCCACC 
CCACCTGTCC ACACTGAAGC CAATTTCTCT GCAAACACAC TCTCTGTACC TCGCTGGTCA 
CCACAGATCC CACGCAGAGA CCTTGGCAAT TCCATCAAGC ACAGGTTTTC CACCAAGTAC 
TGGATGTCTC AGACGTGCAC GGTCTGCGGG AAAGGGATGC TTTTTGGCCT CAAGTGTAAA 
AACTGCAAGT TAAAGTGCCA CAATAAATGC ACCAAAGAAG CCCCGCCCTG TCACCTCCTG 
ATTATCCACC GAGGAGCAAG GTTAGTGCGA ACGGAGTCGG TGCCCTGTGA CATCAACAAC 
CCGGTCCGGA AGCCTGCCCG ATATTCAGAT CTCCACATCA GTCAGACTCT CCCCAAAACC 
AACAAAATCA ACAAGGACCA CATCCCTGTC CCTTACCAGC CAGACTCCAG CAGCAATCCC 
TCCTCCACGA CGTCCTCCAC GCCCTCCTCA CCGGCACCCC CCCTCCCTCC CAGCGCCACG 
CCGCCTTCTC CCCTGCACCC CTCCCCGCAG TGCCCGAGAC AGAAAAAGAA CTTCAACCTG 
CCAGCTTCCC ACTACTACAA ATACAAGCAA CAGTTCATCT TCCCAGATGT GGTGCCTGTA 
CCAGAGACAC CCACCCGGGC ACCCCAAGTC ATCCTGCATC CTGTCACCTC AAATACAATC 
TTGGAAGGAA ACCCATTACT TCAAATTGAA GTGGAACCAA CCTCCGAGAA CGAAGAGAGC 
CACAATGAAG CAGAGGAGTC CGAGGACGAG TTTGAAGAGA TGAACCTATC CCTCCTCTCA 
GCCCGGAGCT TCCCACGCAA AGCCAGCCAG ACCAGCATCT TCCTTCAGGA GTGGGACATC 
CCGTTTGAAC AGCTAGAGAT TGGCGAGCTC ATCGGGAAGG GTCGCTTCGG GCAGGTTTAC 
CACGGACGCT GGCACGGCGA GGTGGCCATC CGGTTGATTG ACATAGAGAG GGACAATGAG 
GACCAGCTTA AGGCCTTCAA GCGGGAAGTG ATGGCCTACA GGCAGACGAG GCACGAGAAC 
GTGGTGCTCT TCATGGGAGC CTGCATGAGC CCACCCCACT TGGCCATCAT CACCAGCCTC 
TGTAAGGGAC GGACACTCTA CTCTGTGGTG AGGGACGCCA AGATTGTCTT GGATGTCAAC 
AAAACCAGGC AGATCGCGCA AGAGATTGTA AAGGGCATGG GCTACCTCCA CGCCAAGGGG 
ATCCTTCATA AGGACCTCAA ATCGAAGAAC GTCTTCTATG ACAACGGCAA AGTGGTCATC 
ACAGACTTTG GGCTCTTCAG CATTTCCGGG GTGCTGCAGG CTGGCCGGCG GGACGACAAA 
CTGCGGATCC AGAACGGCTG GCTGTGTCAC CTGGCCCCAG AAATCATCCG CCAGCTGTCC 
CCTGACACAG AAGAGGACAA GCTCCCATTC TCCAAGCACT CGGATGTCTT TGCGCTTGGC 
ACCATCTGGT ATGAGCTCCA CGCCAGGGAG TGGCCATTCA AGACCCAACC AGCAGAGGCA 
ATCATTTGGC AGATGGGCAC GGGAATGAAG CCCAACCTCA GCCAGATTGG CATGGGAAAA 
GAAATCTCGG ACATACTGCT CTTCTGCTGG GCCTTCGAGC AGGAAGAGCG GCCCACCTTT 
ACCAAGCTCA TGGACATGCT AGAGAAACTG CCAAAGCGAA ACCGCCGCCT GTCGCACCCC 
GGACATTTCT GGAAGTCTGC AGAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ksr2 ( XM_006530394.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530457.1 Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X2, mRNA.

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CloneID	OMu35947
Clone ID Related Accession (Same CDS sequence)	XM_006530394.3 , XM_006530394.4
Accession Version	XM_006530394.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinase suppressor of Ras 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530394.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGCA CCCTGTGGAG GTTGGTCCTC AGCCCTAGTC CCAAGCCTCC TTCAGAGAGT 
CTACACCAGG CCCACCACAG AGGCGAGAGA ATTTTTGGAG CCAAGAAGAA GAGCAAACCC 
TTGAACCTCA AGATCCACAG CGGCGTGGGC AGCTGCGAGA ACATCCCCGC CCAGCAACGC 
TCTCCGCTCC TGTCCGAGCG CTCCTTGCGG TCCTTCTTTG TGGGCCACGG GCCCTTCCTG 
CCCTCCACCC CACCTGTCCA CACTGAAGCC AATTTCTCTG CAAACACACT CTCTGTACCT 
CGCTGGTCAC CACAGATCCC ACGCAGAGAC CTTGGCAATT CCATCAAGCA CAGGTTTTCC 
ACCAAGTACT GGATGTCTCA GACGTGCACG GTCTGCGGGA AAGGGATGCT TTTTGGCCTC 
AAGTGTAAAA ACTGCAAGTT AAAGTGCCAC AATAAATGCA CCAAAGAAGC CCCGCCCTGT 
CACCTCCTGA TTATCCACCG AGGAGACCCA GCAAGGTTAG TGCGAACGGA GTCGGTGCCC 
TGTGACATCA ACAACCCGGT CCGGAAGCCT GCCCGATATT CAGATCTCCA CATCAGTCAG 
ACTCTCCCCA AAACCAACAA AATCAACAAG GACCACATCC CTGTCCCTTA CCAGCCAGAC 
TCCAGCAGCA ATCCCTCCTC CACGACGTCC TCCACGCCCT CCTCACCGGC ACCCCCCCTC 
CCTCCCAGCG CCACGCCGCC TTCTCCCCTG CACCCCTCCC CGCAGTGCCC GAGACAGAAA 
AAGAACTTCA ACCTGCCAGC TTCCCACTAC TACAAATACA AGCAACAGTT CATCTTCCCA 
GATGTGGTGC CTGTACCAGA GACACCCACC CGGGCACCCC AAGTCATCCT GCATCCTGTC 
ACCTCAAATA CAATCTTGGA AGGAAACCCA TTACTTCAAA TTGAAGTGGA ACCAACCTCC 
GAGAACGAAG AGAGCCACAA TGAAGCAGAG GAGTCCGAGG ACGAGTTTGA AGAGATGAAC 
CTATCCCTCC TCTCAGCCCG GAGCTTCCCA CGCAAAGCCA GCCAGACCAG CATCTTCCTT 
CAGGAGTGGG ACATCCCGTT TGAACAGCTA GAGATTGGCG AGCTCATCGG GAAGGGTCGC 
TTCGGGCAGG TTTACCACGG ACGCTGGCAC GGCGAGGTGG CCATCCGGTT GATTGACATA 
GAGAGGGACA ATGAGGACCA GCTTAAGGCC TTCAAGCGGG AAGTGATGGC CTACAGGCAG 
ACGAGGCACG AGAACGTGGT GCTCTTCATG GGAGCCTGCA TGAGCCCACC CCACTTGGCC 
ATCATCACCA GCCTCTGTAA GGGACGGACA CTCTACTCTG TGGTGAGGGA CGCCAAGATT 
GTCTTGGATG TCAACAAAAC CAGGCAGATC GCGCAAGAGA TTGTAAAGGG CATGGGCTAC 
CTCCACGCCA AGGGGATCCT TCATAAGGAC CTCAAATCGA AGAACGTCTT CTATGACAAC 
GGCAAAGTGG TCATCACAGA CTTTGGGCTC TTCAGCATTT CCGGGGTGCT GCAGGCTGGC 
CGGCGGGACG ACAAACTGCG GATCCAGAAC GGCTGGCTGT GTCACCTGGC CCCAGAAATC 
ATCCGCCAGC TGTCCCCTGA CACAGAAGAG GACAAGCTCC CATTCTCCAA GCACTCGGAT 
GTCTTTGCGC TTGGCACCAT CTGGTATGAG CTCCACGCCA GGGAGTGGCC ATTCAAGACC 
CAACCAGCAG AGGCAATCAT TTGGCAGATG GGCACGGGAA TGAAGCCCAA CCTCAGCCAG 
ATTGGCATGG GAAAAGAAAT CTCGGACATA CTGCTCTTCT GCTGGGCCTT CGAGCAGGAA 
GAGCGGCCCA CCTTTACCAA GCTCATGGAC ATGCTAGAGA AACTGCCAAA GCGAAACCGC 
CGCCTGTCGC ACCCCGGACA TTTCTGGAAG TCTGCAGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530457.1
CDS	706..2670
Translation	MICTLWRLVLSPSPKPPSESLHQAHHRGERIFGAKKKSKPLNLKIHSGVGSCENIPAQQRSPLLSERSLRSFFVGHGPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGDPARLVRTESVPCDINNPVRKPARYSDLHISQTLPKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCPRQKKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNTILEGNPLLQIEVEPTSENEESHNEAEESEDEFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRRDDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL

Target ORF information:

RefSeq Version	XM_006530394.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530394.4

ORF Insert Sequence:

ATGATCTGCA CCCTGTGGAG GTTGGTCCTC AGCCCTAGTC CCAAGCCTCC TTCAGAGAGT 
CTACACCAGG CCCACCACAG AGGCGAGAGA ATTTTTGGAG CCAAGAAGAA GAGCAAACCC 
TTGAACCTCA AGATCCACAG CGGCGTGGGC AGCTGCGAGA ACATCCCCGC CCAGCAACGC 
TCTCCGCTCC TGTCCGAGCG CTCCTTGCGG TCCTTCTTTG TGGGCCACGG GCCCTTCCTG 
CCCTCCACCC CACCTGTCCA CACTGAAGCC AATTTCTCTG CAAACACACT CTCTGTACCT 
CGCTGGTCAC CACAGATCCC ACGCAGAGAC CTTGGCAATT CCATCAAGCA CAGGTTTTCC 
ACCAAGTACT GGATGTCTCA GACGTGCACG GTCTGCGGGA AAGGGATGCT TTTTGGCCTC 
AAGTGTAAAA ACTGCAAGTT AAAGTGCCAC AATAAATGCA CCAAAGAAGC CCCGCCCTGT 
CACCTCCTGA TTATCCACCG AGGAGACCCA GCAAGGTTAG TGCGAACGGA GTCGGTGCCC 
TGTGACATCA ACAACCCGGT CCGGAAGCCT GCCCGATATT CAGATCTCCA CATCAGTCAG 
ACTCTCCCCA AAACCAACAA AATCAACAAG GACCACATCC CTGTCCCTTA CCAGCCAGAC 
TCCAGCAGCA ATCCCTCCTC CACGACGTCC TCCACGCCCT CCTCACCGGC ACCCCCCCTC 
CCTCCCAGCG CCACGCCGCC TTCTCCCCTG CACCCCTCCC CGCAGTGCCC GAGACAGAAA 
AAGAACTTCA ACCTGCCAGC TTCCCACTAC TACAAATACA AGCAACAGTT CATCTTCCCA 
GATGTGGTGC CTGTACCAGA GACACCCACC CGGGCACCCC AAGTCATCCT GCATCCTGTC 
ACCTCAAATA CAATCTTGGA AGGAAACCCA TTACTTCAAA TTGAAGTGGA ACCAACCTCC 
GAGAACGAAG AGAGCCACAA TGAAGCAGAG GAGTCCGAGG ACGAGTTTGA AGAGATGAAC 
CTATCCCTCC TCTCAGCCCG GAGCTTCCCA CGCAAAGCCA GCCAGACCAG CATCTTCCTT 
CAGGAGTGGG ACATCCCGTT TGAACAGCTA GAGATTGGCG AGCTCATCGG GAAGGGTCGC 
TTCGGGCAGG TTTACCACGG ACGCTGGCAC GGCGAGGTGG CCATCCGGTT GATTGACATA 
GAGAGGGACA ATGAGGACCA GCTTAAGGCC TTCAAGCGGG AAGTGATGGC CTACAGGCAG 
ACGAGGCACG AGAACGTGGT GCTCTTCATG GGAGCCTGCA TGAGCCCACC CCACTTGGCC 
ATCATCACCA GCCTCTGTAA GGGACGGACA CTCTACTCTG TGGTGAGGGA CGCCAAGATT 
GTCTTGGATG TCAACAAAAC CAGGCAGATC GCGCAAGAGA TTGTAAAGGG CATGGGCTAC 
CTCCACGCCA AGGGGATCCT TCATAAGGAC CTCAAATCGA AGAACGTCTT CTATGACAAC 
GGCAAAGTGG TCATCACAGA CTTTGGGCTC TTCAGCATTT CCGGGGTGCT GCAGGCTGGC 
CGGCGGGACG ACAAACTGCG GATCCAGAAC GGCTGGCTGT GTCACCTGGC CCCAGAAATC 
ATCCGCCAGC TGTCCCCTGA CACAGAAGAG GACAAGCTCC CATTCTCCAA GCACTCGGAT 
GTCTTTGCGC TTGGCACCAT CTGGTATGAG CTCCACGCCA GGGAGTGGCC ATTCAAGACC 
CAACCAGCAG AGGCAATCAT TTGGCAGATG GGCACGGGAA TGAAGCCCAA CCTCAGCCAG 
ATTGGCATGG GAAAAGAAAT CTCGGACATA CTGCTCTTCT GCTGGGCCTT CGAGCAGGAA 
GAGCGGCCCA CCTTTACCAA GCTCATGGAC ATGCTAGAGA AACTGCCAAA GCGAAACCGC 
CGCCTGTCGC ACCCCGGACA TTTCTGGAAG TCTGCAGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ksr2 ( XM_006530395.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530458.1 Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu35948
Clone ID Related Accession (Same CDS sequence)	XM_006530395.4 , XM_006530395.3
Accession Version	XM_006530395.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinase suppressor of Ras 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530395.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATA GGATGTTGCC GACCAACAGC AACAATGTAT CAGGCGCTTT GCCGAACACA 
CTCTCTGTAC CTCGCTGGTC ACCACAGATC CCACGCAGAG ACCTTGGCAA TTCCATCAAG 
CACAGGTTTT CCACCAAGTA CTGGATGTCT CAGACGTGCA CGGTCTGCGG GAAAGGGATG 
CTTTTTGGCC TCAAGTGTAA AAACTGCAAG TTAAAGTGCC ACAATAAATG CACCAAAGAA 
GCCCCGCCCT GTCACCTCCT GATTATCCAC CGAGGAGACC CAGCAAGGTT AGTGCGAACG 
GAGTCGGTGC CCTGTGACAT CAACAACCCG GTCCGGAAGC CTGCCCGATA TTCAGATCTC 
CACATCAGTC AGACTCTCCC CAAAACCAAC AAAATCAACA AGGACCACAT CCCTGTCCCT 
TACCAGCCAG ACTCCAGCAG CAATCCCTCC TCCACGACGT CCTCCACGCC CTCCTCACCG 
GCACCCCCCC TCCCTCCCAG CGCCACGCCG CCTTCTCCCC TGCACCCCTC CCCGCAGTGC 
CCGAGACAGA AAAAGAACTT CAACCTGCCA GCTTCCCACT ACTACAAATA CAAGCAACAG 
TTCATCTTCC CAGATGTGGT GCCTGTACCA GAGACACCCA CCCGGGCACC CCAAGTCATC 
CTGCATCCTG TCACCTCAAA TACAATCTTG GAAGGAAACC CATTACTTCA AATTGAAGTG 
GAACCAACCT CCGAGAACGA AGAGAGCCAC AATGAAGCAG AGGAGTCCGA GGACGAGTTT 
GAAGAGATGA ACCTATCCCT CCTCTCAGCC CGGAGCTTCC CACGCAAAGC CAGCCAGACC 
AGCATCTTCC TTCAGGAGTG GGACATCCCG TTTGAACAGC TAGAGATTGG CGAGCTCATC 
GGGAAGGGTC GCTTCGGGCA GGTTTACCAC GGACGCTGGC ACGGCGAGGT GGCCATCCGG 
TTGATTGACA TAGAGAGGGA CAATGAGGAC CAGCTTAAGG CCTTCAAGCG GGAAGTGATG 
GCCTACAGGC AGACGAGGCA CGAGAACGTG GTGCTCTTCA TGGGAGCCTG CATGAGCCCA 
CCCCACTTGG CCATCATCAC CAGCCTCTGT AAGGGACGGA CACTCTACTC TGTGGTGAGG 
GACGCCAAGA TTGTCTTGGA TGTCAACAAA ACCAGGCAGA TCGCGCAAGA GATTGTAAAG 
GGCATGGGCT ACCTCCACGC CAAGGGGATC CTTCATAAGG ACCTCAAATC GAAGAACGTC 
TTCTATGACA ACGGCAAAGT GGTCATCACA GACTTTGGGC TCTTCAGCAT TTCCGGGGTG 
CTGCAGGCTG GCCGGCGGGA CGACAAACTG CGGATCCAGA ACGGCTGGCT GTGTCACCTG 
GCCCCAGAAA TCATCCGCCA GCTGTCCCCT GACACAGAAG AGGACAAGCT CCCATTCTCC 
AAGCACTCGG ATGTCTTTGC GCTTGGCACC ATCTGGTATG AGCTCCACGC CAGGGAGTGG 
CCATTCAAGA CCCAACCAGC AGAGGCAATC ATTTGGCAGA TGGGCACGGG AATGAAGCCC 
AACCTCAGCC AGATTGGCAT GGGAAAAGAA ATCTCGGACA TACTGCTCTT CTGCTGGGCC 
TTCGAGCAGG AAGAGCGGCC CACCTTTACC AAGCTCATGG ACATGCTAGA GAAACTGCCA 
AAGCGAAACC GCCGCCTGTC GCACCCCGGA CATTTCTGGA AGTCTGCAGA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530458.1
CDS	576..2312
Translation	MMNRMLPTNSNNVSGALPNTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGDPARLVRTESVPCDINNPVRKPARYSDLHISQTLPKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCPRQKKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNTILEGNPLLQIEVEPTSENEESHNEAEESEDEFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRRDDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL

Target ORF information:

RefSeq Version	XM_006530395.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinase suppressor of ras 2 (Ksr2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530395.4

ORF Insert Sequence:

ATGATGAATA GGATGTTGCC GACCAACAGC AACAATGTAT CAGGCGCTTT GCCGAACACA 
CTCTCTGTAC CTCGCTGGTC ACCACAGATC CCACGCAGAG ACCTTGGCAA TTCCATCAAG 
CACAGGTTTT CCACCAAGTA CTGGATGTCT CAGACGTGCA CGGTCTGCGG GAAAGGGATG 
CTTTTTGGCC TCAAGTGTAA AAACTGCAAG TTAAAGTGCC ACAATAAATG CACCAAAGAA 
GCCCCGCCCT GTCACCTCCT GATTATCCAC CGAGGAGACC CAGCAAGGTT AGTGCGAACG 
GAGTCGGTGC CCTGTGACAT CAACAACCCG GTCCGGAAGC CTGCCCGATA TTCAGATCTC 
CACATCAGTC AGACTCTCCC CAAAACCAAC AAAATCAACA AGGACCACAT CCCTGTCCCT 
TACCAGCCAG ACTCCAGCAG CAATCCCTCC TCCACGACGT CCTCCACGCC CTCCTCACCG 
GCACCCCCCC TCCCTCCCAG CGCCACGCCG CCTTCTCCCC TGCACCCCTC CCCGCAGTGC 
CCGAGACAGA AAAAGAACTT CAACCTGCCA GCTTCCCACT ACTACAAATA CAAGCAACAG 
TTCATCTTCC CAGATGTGGT GCCTGTACCA GAGACACCCA CCCGGGCACC CCAAGTCATC 
CTGCATCCTG TCACCTCAAA TACAATCTTG GAAGGAAACC CATTACTTCA AATTGAAGTG 
GAACCAACCT CCGAGAACGA AGAGAGCCAC AATGAAGCAG AGGAGTCCGA GGACGAGTTT 
GAAGAGATGA ACCTATCCCT CCTCTCAGCC CGGAGCTTCC CACGCAAAGC CAGCCAGACC 
AGCATCTTCC TTCAGGAGTG GGACATCCCG TTTGAACAGC TAGAGATTGG CGAGCTCATC 
GGGAAGGGTC GCTTCGGGCA GGTTTACCAC GGACGCTGGC ACGGCGAGGT GGCCATCCGG 
TTGATTGACA TAGAGAGGGA CAATGAGGAC CAGCTTAAGG CCTTCAAGCG GGAAGTGATG 
GCCTACAGGC AGACGAGGCA CGAGAACGTG GTGCTCTTCA TGGGAGCCTG CATGAGCCCA 
CCCCACTTGG CCATCATCAC CAGCCTCTGT AAGGGACGGA CACTCTACTC TGTGGTGAGG 
GACGCCAAGA TTGTCTTGGA TGTCAACAAA ACCAGGCAGA TCGCGCAAGA GATTGTAAAG 
GGCATGGGCT ACCTCCACGC CAAGGGGATC CTTCATAAGG ACCTCAAATC GAAGAACGTC 
TTCTATGACA ACGGCAAAGT GGTCATCACA GACTTTGGGC TCTTCAGCAT TTCCGGGGTG 
CTGCAGGCTG GCCGGCGGGA CGACAAACTG CGGATCCAGA ACGGCTGGCT GTGTCACCTG 
GCCCCAGAAA TCATCCGCCA GCTGTCCCCT GACACAGAAG AGGACAAGCT CCCATTCTCC 
AAGCACTCGG ATGTCTTTGC GCTTGGCACC ATCTGGTATG AGCTCCACGC CAGGGAGTGG 
CCATTCAAGA CCCAACCAGC AGAGGCAATC ATTTGGCAGA TGGGCACGGG AATGAAGCCC 
AACCTCAGCC AGATTGGCAT GGGAAAAGAA ATCTCGGACA TACTGCTCTT CTGCTGGGCC 
TTCGAGCAGG AAGAGCGGCC CACCTTTACC AAGCTCATGG ACATGCTAGA GAAACTGCCA 
AAGCGAAACC GCCGCCTGTC GCACCCCGGA CATTTCTGGA AGTCTGCAGA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Kinase Suppressor of Ras 2 (KSR2) expression in the brain regulates energy balance and glucose homeostasis.
Molecular metabolism6(2)194-205(2017 Feb)
Guo L,Costanzo-Garvey DL,Smith DR,Neilsen BK,MacDonald RG,Lewis RE

Cell non-autonomous regulation of hepatic IGF-1 and neonatal growth by Kinase Suppressor of Ras 2 (KSR2).
Scientific reports632093(2016 Aug)
Guo L,Costanzo-Garvey DL,Smith DR,Zavorka ME,Venable-Kang M,MacDonald RG,Lewis RE

Ultrastructural study of spermatogenesis in KSR2 deficient mice.
Transgenic research24(4)741-51(2015 Aug)
Moretti E,Collodel G,Mazzi L,Russo I,Giurisato E

Identification of a truncated kinase suppressor of Ras 2 mRNA in sperm.
FEBS open bio4420-5(2014)
Guo L,Volle DJ,Lewis RE

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Ksr2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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