Dgkk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dgkk
Entrez Gene ID	331374
Full Name	diacylglycerol kinase kappa
Synonyms	Gm360
General protein information	Preferred Names diacylglycerol kinase kappa Names diacylglycerol kinase kappa
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X A1.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_177914.3	NP_808582.3	diacylglycerol kinase kappa

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DGKK	XP_528979.2
Canis lupus familiaris (dog)	DGKK	XP_549004.3
Homo sapiens (human)	DGKK	NP_001013764.1
	DGKK	XP_001083870.1
Bos taurus (cattle)	DGKK	XP_002700155.2
Rattus norvegicus (Norway rat)	Dgkk	XP_346277.2
Mus musculus (house mouse)	Dgkk	NP_808582.3

Gene Ontology
Bibliography

Related articles in PubMed

Fragile X Mental Retardation Protein (FMRP) controls diacylglycerol kinase activity in neurons.
Tabet R, Moutin E, Becker JA, Heintz D, Fouillen L, Flatter E, Kr??el W, Alunni V, Koebel P, Demb?l? D, Tassone F, Bardoni B, Mandel JL, Vitale N, Muller D, Le Merrer J, Moine H
Proceedings of the National Academy of Sciences of the United States of America113(26)E3619-28(2016 06)

Expression Analysis of DGKK during External Genitalia Formation.
Shen J, Liu B, Sinclair A, Cunha G, Baskin LS, Choudhry S
The Journal of urology194(6)1728-36(2015 Dec)

Diacylglycerol kinase delta regulates protein kinase C and epidermal growth factor receptor signaling.
Crotty T, Cai J, Sakane F, Taketomi A, Prescott SM, Topham MK
Proceedings of the National Academy of Sciences of the United States of America103(42)15485-90(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of Dgkkappa in neurons is able to rescue the dendritic spine defects of the Fragile X Mental Retardation 1 gene KO neurons.
Title: Fragile X Mental Retardation Protein (FMRP) controls diacylglycerol kinase activity in neurons.

The following Dgkk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dgkk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21067	NM_177914.3 Latest version!	Mus musculus diacylglycerol kinase kappa (Dgkk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$657.30 $939.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dgkk ( NM_177914.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808582.3 Mus musculus diacylglycerol kinase kappa (Dgkk), mRNA.

Price & Availability↑

CloneID	OMu21067
Clone ID Related Accession (Same CDS sequence)	NM_177914.3
Accession Version	NM_177914.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-23
Organism	Mus musculus(house mouse)
Product	diacylglycerol kinase kappa
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC075627.1, AL671962.5 and AW121906.1. On or before Jun 8, 2008 this sequence version replaced XM_001481246.1, NM_177914.2. ##Evidence-Data-START## Transcript exon combination :: BC075627.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164136 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAGGG CCCCAGAGTC CTGGCCAGAG CCAACTCCAG AGTCCTGTCT AAGAATGAGT 
CCAGCACTAC ATCTGTTGCA GGTAGTTCCT GGAGAGAAAA GTGGAACAGC ATCTCCAGTG 
CTATTGCCAT TGCCGTCGCC CAAACCGCCA ATAACGTGGT CCAGAATAAA GAAAATACTG 
AAGGAAGGAC CCCTGCTGAA GAACTGTAAC TCCTTCAGAA GATGGAAGCT TAGATACTGT 
CTGGTTCAAG GGCAGAAGCT CCACTTTGCA CACCATCCTT CGTTTGCACA CTTTGAAACA 
ATTGATCTGT CTCAAGTTGT TTTGGCTGAA AGTAGCTGCA GAAATCTTTG CCATGGTTTT 
TGTGTTATCA CACCACATCG AAAAGTGTCT CTAGTTGCAC CCACTCGGCA AGACATGGAA 
GAATGGATTA ACATCATAAA AACTGTCCAA CAAGGAGAAA TCCGTCAGAT CCCTGCAGCC 
GAAAACAACC CCTTTCTTGT CGGAATGCAC TACTGGTATT CCAGTAGTAA TCCCCGGAGT 
CACTTCTGCA ATGTTTGTCG AGAGAACATT CCTGCTTTAT CTCGAGATGC CGTCACCTGT 
GAAGTCTGTC AGGTGAAATC TCATAAATTC TGTGCTTTGA GAGCAAACAA AGACTGTAAA 
TGGAATACTT TATCCGTGAC TGATGACCTT CTTCTACCCG CTGATGAAAT ACAAACAATG 
CCGCATCAGT GGGTAGAAGG AAATATACCC GCTGGCTCTC AATGTGTCGT ATGTCACAAG 
AGCTGCGGTA GTCATCACAG ACTTCAGGAT TTCCGCTGCC TCTGGTGCGG CTCTACGGTG 
CACGGGGCCT GCCAGAAGCG GTTTTCCAAG GAATGTTCCT TCGGAAGTCG TCGCTCGTCC 
ATCGTGCCAC CAACGGCACT GAGCGACCCT AGGGGCGATG GCCAGCTAGT AGTATCGCCT 
GACTTCTGGA ATCTTGACTG GCCACTGACT TGTTCCTGTC CCTTGCTCAT CTTCATCAAC 
TCTAAAAGTG GAGATCATCA AGGGATCATC TTTCTCCGGA AATTCAAACA ATACCTTAAT 
CCGTCTCAAG TATTCGACCT GGCGAAGGGT GGACCTGAAG CAGGCATCGC TATGTTCAAG 
AACTTTGCCC GTTTTCGTGT TCTGGTTTGT GGTGGAGATG GCAGTGTGAG CTGGGTCTTG 
TCTACGATTG ATGCCTATGG ATTGCATGAT AGGTGTCAGC TGGCGATCAT CCCACTTGGA 
ACTGGTAATG ATCTCGCTCG TGTCCTGGGC TGGGGAGCAG TCTGGAGTAA AGGCACATCA 
CCACTGGATA TTCTCAGCAG AGTGGAGCAG GCTCACGTGA GGATTTTAGA CAGATGGAGT 
GTGATGATCC GTGAGACCCC CAGACAAGCC CCGCTGCTGA AAGGACAAGT GGCAATGGAT 
ATACCAAGAT TTGAGGCTGC TGCCATCAAG AACGTAGAGT CTGCAACCAC TGAGTTGAAC 
AAAATTCTGA AGGCCAAATA TCCCACGGAG ATGGTCATCG CCACAAGATT CCTGTGCTCA 
GCAGTGGAAG ATTTTGTGGA AGATATTGTA AAGGCCTGGC ACCAAATAAA ACAGAACAGC 
ACAGCAGTGG AGTCTGTGAT TTTGAAAAGC GACTTAATGT ATGATAAACT CAGTGTCCTT 
ATTGATCTCC TGGCTGAGGA TGCAGTAGCT GCCTCCGCTG AGAGGACAGC CACAGCATAT 
GGAAGCAGAA GCCAAGCAGA TGGAAAACCC TTCGTGCCTC AACTAGACCA CATAGCAAGG 
GCCAAACTGG AGTTGGCTGA AAGGGCTCAG AAACTCCAGC AGTCCTTGAA ACTCATCATA 
TTCCAGGTCG AACAAGTTCT GGATGAAGAA AGCAGACAGA GCTTATCAGT TAAGAACTTT 
ACTTCATCTT TGTTCCTGGG AGACGGAGAC GACGACGACT CAGATGACTA TGATCAGAGC 
CCTAGACACC GTTCTCGTTG TGACATCTTA TGTTCTATAC CTTCTCTGAG AAATGAAGAC 
CTAGATAACC TTGACTTAGA ACACTTACAC ATTGCACCTG AAACGATACG CTTCAAAGAA 
AAGTGTGTCA TGAACAACTA CTTTGGAATT GGACTAGACG CAAAAATCTC TCTGGAGTTT 
AATTCCAGAA GAGAAGAACA TCCAGAACAA TACAATAGCC GCCTCAAGAA CAAAATATGG 
TATGGTCTTC TGGGAAGCAA GGAATTACTG CAACGCTCTT ACAGGAAATT GGAAGAGCGA 
ATACACCTCG AGTGTGATGG AGAAGCTGTC TCCTTGCCAA ACCTTCAAGG CATCGTAGTG 
CTCAACATTA CGAGCTACGC TGGAGGCGTC AACTTTTGGG GAAGAAACAG AGCAACCACA 
GAATATGATG TCCCTGCAAT CAATGATGGG AAGCTAGAGG TTGTGGCAAT CTTTGGTTCT 
GTGCAGATGG CCATGTCTCG TATCGTCAAC CTGCAACAAC ATCGAATTGC GCAGTGCCAT 
GAGGTGGTAA TAACTATTGA TGGTGAAGAC GGTGTCCCTG TGCAAGTGGA TGGGGAAGCC 
TGGATTCAGA AGCCAGGCCT TATCAAGATT AAATACAAGA ATGTTGCCCA GATGTTGATG 
AGAGATAGGG ACTTTGAGAA CTCAATGAAA ACCTGGGAAT CTAAGCATAC TGAAATCCAA 
GCTGTCCAAC CACCCCACCT GGATTTCCAG GAATCTCAAG ACAGCCTCTC TGATGGAGAG 
TATGCCCAGA TGCAGCACTT GGCTCGGCTT GCAGAAAACC TCATTAGCAG ACTTACTGAC 
CTAAGCAAGG TCCACCAGCA CGTGTCTGTC CTCATGGATT CTGTGAATGC CAGTGCTAAC 
ATACTGAATG ATGTGTTCTA TAGCCAAGAC AGTGGTAACG AAGCAGGCGC AGCTTCCTGC 
ATTCCCATTG AGACTCTAAG CAGAACTGAT GCAGTAGATG TTACATTTAG TCTTAAAGGG 
CTCTACGATG ACACCAAAGC TTTCCTGGAT GAAAACTTGT TGAGAGATGC TGAGGATCGG 
GCCATGCTAC AAACTGCCTT GGATGCCATG AATACAGAGT TAAGAAGGAT ACTGGCAATT 
GGCTGGCTGA GTCAAATCTT CTTTCCAGAG GAACAGGCTT CTGACACTCG CAGTTTAAGT 
CGTAGGTTTA GAATAAAATT CCCCAAGCTG GGGAAGAAGA AACAACGAGA AGAGGGAGAA 
AAGCCTAAAT CAAGCCAAAG ATTCCCTGGT TTTCTTGGCA AGTTCTGGCG TCGAAGAAAT 
CGTAGCAATC GAGCAAAAGC TGATGACCCT CCAACACCTT CAAGTTCTCA ACTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808582.3
CDS	161..3517
Translation	MERAPESWPEPTPESCLRMSPALHLLQVVPGEKSGTASPVLLPLPSPKPPITWSRIKKILKEGPLLKNCNSFRRWKLRYCLVQGQKLHFAHHPSFAHFETIDLSQVVLAESSCRNLCHGFCVITPHRKVSLVAPTRQDMEEWINIIKTVQQGEIRQIPAAENNPFLVGMHYWYSSSNPRSHFCNVCRENIPALSRDAVTCEVCQVKSHKFCALRANKDCKWNTLSVTDDLLLPADEIQTMPHQWVEGNIPAGSQCVVCHKSCGSHHRLQDFRCLWCGSTVHGACQKRFSKECSFGSRRSSIVPPTALSDPRGDGQLVVSPDFWNLDWPLTCSCPLLIFINSKSGDHQGIIFLRKFKQYLNPSQVFDLAKGGPEAGIAMFKNFARFRVLVCGGDGSVSWVLSTIDAYGLHDRCQLAIIPLGTGNDLARVLGWGAVWSKGTSPLDILSRVEQAHVRILDRWSVMIRETPRQAPLLKGQVAMDIPRFEAAAIKNVESATTELNKILKAKYPTEMVIATRFLCSAVEDFVEDIVKAWHQIKQNSTAVESVILKSDLMYDKLSVLIDLLAEDAVAASAERTATAYGSRSQADGKPFVPQLDHIARAKLELAERAQKLQQSLKLIIFQVEQVLDEESRQSLSVKNFTSSLFLGDGDDDDSDDYDQSPRHRSRCDILCSIPSLRNEDLDNLDLEHLHIAPETIRFKEKCVMNNYFGIGLDAKISLEFNSRREEHPEQYNSRLKNKIWYGLLGSKELLQRSYRKLEERIHLECDGEAVSLPNLQGIVVLNITSYAGGVNFWGRNRATTEYDVPAINDGKLEVVAIFGSVQMAMSRIVNLQQHRIAQCHEVVITIDGEDGVPVQVDGEAWIQKPGLIKIKYKNVAQMLMRDRDFENSMKTWESKHTEIQAVQPPHLDFQESQDSLSDGEYAQMQHLARLAENLISRLTDLSKVHQHVSVLMDSVNASANILNDVFYSQDSGNEAGAASCIPIETLSRTDAVDVTFSLKGLYDDTKAFLDENLLRDAEDRAMLQTALDAMNTELRRILAIGWLSQIFFPEEQASDTRSLSRRFRIKFPKLGKKKQREEGEKPKSSQRFPGFLGKFWRRRNRSNRAKADDPPTPSSSQL

Target ORF information:

RefSeq Version	NM_177914.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus diacylglycerol kinase kappa (Dgkk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177914.3

ORF Insert Sequence:

ATGGAGAGGG CCCCAGAGTC CTGGCCAGAG CCAACTCCAG AGTCCTGTCT AAGAATGAGT 
CCAGCACTAC ATCTGTTGCA GGTAGTTCCT GGAGAGAAAA GTGGAACAGC ATCTCCAGTG 
CTATTGCCAT TGCCGTCGCC CAAACCGCCA ATAACGTGGT CCAGAATAAA GAAAATACTG 
AAGGAAGGAC CCCTGCTGAA GAACTGTAAC TCCTTCAGAA GATGGAAGCT TAGATACTGT 
CTGGTTCAAG GGCAGAAGCT CCACTTTGCA CACCATCCTT CGTTTGCACA CTTTGAAACA 
ATTGATCTGT CTCAAGTTGT TTTGGCTGAA AGTAGCTGCA GAAATCTTTG CCATGGTTTT 
TGTGTTATCA CACCACATCG AAAAGTGTCT CTAGTTGCAC CCACTCGGCA AGACATGGAA 
GAATGGATTA ACATCATAAA AACTGTCCAA CAAGGAGAAA TCCGTCAGAT CCCTGCAGCC 
GAAAACAACC CCTTTCTTGT CGGAATGCAC TACTGGTATT CCAGTAGTAA TCCCCGGAGT 
CACTTCTGCA ATGTTTGTCG AGAGAACATT CCTGCTTTAT CTCGAGATGC CGTCACCTGT 
GAAGTCTGTC AGGTGAAATC TCATAAATTC TGTGCTTTGA GAGCAAACAA AGACTGTAAA 
TGGAATACTT TATCCGTGAC TGATGACCTT CTTCTACCCG CTGATGAAAT ACAAACAATG 
CCGCATCAGT GGGTAGAAGG AAATATACCC GCTGGCTCTC AATGTGTCGT ATGTCACAAG 
AGCTGCGGTA GTCATCACAG ACTTCAGGAT TTCCGCTGCC TCTGGTGCGG CTCTACGGTG 
CACGGGGCCT GCCAGAAGCG GTTTTCCAAG GAATGTTCCT TCGGAAGTCG TCGCTCGTCC 
ATCGTGCCAC CAACGGCACT GAGCGACCCT AGGGGCGATG GCCAGCTAGT AGTATCGCCT 
GACTTCTGGA ATCTTGACTG GCCACTGACT TGTTCCTGTC CCTTGCTCAT CTTCATCAAC 
TCTAAAAGTG GAGATCATCA AGGGATCATC TTTCTCCGGA AATTCAAACA ATACCTTAAT 
CCGTCTCAAG TATTCGACCT GGCGAAGGGT GGACCTGAAG CAGGCATCGC TATGTTCAAG 
AACTTTGCCC GTTTTCGTGT TCTGGTTTGT GGTGGAGATG GCAGTGTGAG CTGGGTCTTG 
TCTACGATTG ATGCCTATGG ATTGCATGAT AGGTGTCAGC TGGCGATCAT CCCACTTGGA 
ACTGGTAATG ATCTCGCTCG TGTCCTGGGC TGGGGAGCAG TCTGGAGTAA AGGCACATCA 
CCACTGGATA TTCTCAGCAG AGTGGAGCAG GCTCACGTGA GGATTTTAGA CAGATGGAGT 
GTGATGATCC GTGAGACCCC CAGACAAGCC CCGCTGCTGA AAGGACAAGT GGCAATGGAT 
ATACCAAGAT TTGAGGCTGC TGCCATCAAG AACGTAGAGT CTGCAACCAC TGAGTTGAAC 
AAAATTCTGA AGGCCAAATA TCCCACGGAG ATGGTCATCG CCACAAGATT CCTGTGCTCA 
GCAGTGGAAG ATTTTGTGGA AGATATTGTA AAGGCCTGGC ACCAAATAAA ACAGAACAGC 
ACAGCAGTGG AGTCTGTGAT TTTGAAAAGC GACTTAATGT ATGATAAACT CAGTGTCCTT 
ATTGATCTCC TGGCTGAGGA TGCAGTAGCT GCCTCCGCTG AGAGGACAGC CACAGCATAT 
GGAAGCAGAA GCCAAGCAGA TGGAAAACCC TTCGTGCCTC AACTAGACCA CATAGCAAGG 
GCCAAACTGG AGTTGGCTGA AAGGGCTCAG AAACTCCAGC AGTCCTTGAA ACTCATCATA 
TTCCAGGTCG AACAAGTTCT GGATGAAGAA AGCAGACAGA GCTTATCAGT TAAGAACTTT 
ACTTCATCTT TGTTCCTGGG AGACGGAGAC GACGACGACT CAGATGACTA TGATCAGAGC 
CCTAGACACC GTTCTCGTTG TGACATCTTA TGTTCTATAC CTTCTCTGAG AAATGAAGAC 
CTAGATAACC TTGACTTAGA ACACTTACAC ATTGCACCTG AAACGATACG CTTCAAAGAA 
AAGTGTGTCA TGAACAACTA CTTTGGAATT GGACTAGACG CAAAAATCTC TCTGGAGTTT 
AATTCCAGAA GAGAAGAACA TCCAGAACAA TACAATAGCC GCCTCAAGAA CAAAATATGG 
TATGGTCTTC TGGGAAGCAA GGAATTACTG CAACGCTCTT ACAGGAAATT GGAAGAGCGA 
ATACACCTCG AGTGTGATGG AGAAGCTGTC TCCTTGCCAA ACCTTCAAGG CATCGTAGTG 
CTCAACATTA CGAGCTACGC TGGAGGCGTC AACTTTTGGG GAAGAAACAG AGCAACCACA 
GAATATGATG TCCCTGCAAT CAATGATGGG AAGCTAGAGG TTGTGGCAAT CTTTGGTTCT 
GTGCAGATGG CCATGTCTCG TATCGTCAAC CTGCAACAAC ATCGAATTGC GCAGTGCCAT 
GAGGTGGTAA TAACTATTGA TGGTGAAGAC GGTGTCCCTG TGCAAGTGGA TGGGGAAGCC 
TGGATTCAGA AGCCAGGCCT TATCAAGATT AAATACAAGA ATGTTGCCCA GATGTTGATG 
AGAGATAGGG ACTTTGAGAA CTCAATGAAA ACCTGGGAAT CTAAGCATAC TGAAATCCAA 
GCTGTCCAAC CACCCCACCT GGATTTCCAG GAATCTCAAG ACAGCCTCTC TGATGGAGAG 
TATGCCCAGA TGCAGCACTT GGCTCGGCTT GCAGAAAACC TCATTAGCAG ACTTACTGAC 
CTAAGCAAGG TCCACCAGCA CGTGTCTGTC CTCATGGATT CTGTGAATGC CAGTGCTAAC 
ATACTGAATG ATGTGTTCTA TAGCCAAGAC AGTGGTAACG AAGCAGGCGC AGCTTCCTGC 
ATTCCCATTG AGACTCTAAG CAGAACTGAT GCAGTAGATG TTACATTTAG TCTTAAAGGG 
CTCTACGATG ACACCAAAGC TTTCCTGGAT GAAAACTTGT TGAGAGATGC TGAGGATCGG 
GCCATGCTAC AAACTGCCTT GGATGCCATG AATACAGAGT TAAGAAGGAT ACTGGCAATT 
GGCTGGCTGA GTCAAATCTT CTTTCCAGAG GAACAGGCTT CTGACACTCG CAGTTTAAGT 
CGTAGGTTTA GAATAAAATT CCCCAAGCTG GGGAAGAAGA AACAACGAGA AGAGGGAGAA 
AAGCCTAAAT CAAGCCAAAG ATTCCCTGGT TTTCTTGGCA AGTTCTGGCG TCGAAGAAAT 
CGTAGCAATC GAGCAAAAGC TGATGACCCT CCAACACCTT CAAGTTCTCA ACTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fragile X Mental Retardation Protein (FMRP) controls diacylglycerol kinase activity in neurons.
Proceedings of the National Academy of Sciences of the United States of America113(26)E3619-28(2016 06)
Tabet R,Moutin E,Becker JA,Heintz D,Fouillen L,Flatter E,Kr??el W,Alunni V,Koebel P,Demb?l? D,Tassone F,Bardoni B,Mandel JL,Vitale N,Muller D,Le Merrer J,Moine H

Expression Analysis of DGKK during External Genitalia Formation.
The Journal of urology194(6)1728-36(2015 Dec)
Shen J,Liu B,Sinclair A,Cunha G,Baskin LS,Choudhry S

Diacylglycerol kinase delta regulates protein kinase C and epidermal growth factor receptor signaling.
Proceedings of the National Academy of Sciences of the United States of America103(42)15485-90(2006 Oct)
Crotty T,Cai J,Sakane F,Taketomi A,Prescott SM,Topham MK

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Dgkk cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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