Lhfpl5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lhfpl5
Entrez Gene ID	328789
Full Name	lipoma HMGIC fusion partner-like 5
Synonyms	9330179O15Rik,Tmhs,hscy
General protein information	Preferred Names lipoma HMGIC fusion partner-like 5 Names LHFPL tetraspan subfamily member 5 protein tetraspan membrane protein of hair cell stereocilia lipoma HMGIC fusion partner-like 5 protein tetraspan transmembrane protein, hair cell stereocilia
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A3.3\|17 14.77 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006524433.3	XP_006524496.1	tetraspan membrane protein of hair cell stereocilia isoform X1
NM_026571.2	NP_080847.2	LHFPL tetraspan subfamily member 5 protein
XM_006524433.4	XP_006524496.1	LHFPL tetraspan subfamily member 5 protein isoform X1

Pathways from BioSystems

Homologies

	LOC718952	XP_001112357.2
Canis lupus familiaris (dog)	LHFPL5	XP_005627321.1
Rattus norvegicus (Norway rat)	Lhfpl5	NP_001013928.1
Gallus gallus (chicken)	LHFPL5	NP_989729.1
Danio rerio (zebrafish)	lhfpl5a	XP_686620.5
Homo sapiens (human)	LHFPL5	NP_872354.1
Caenorhabditis elegans (roundworm)	F26D10.11	NP_503066.1
Bos taurus (cattle)	LHFPL5	NP_001192875.1
Mus musculus (house mouse)	Lhfpl5	NP_080847.2
Xenopus tropicalis (tropical clawed frog)	lhfpl5	NP_001017269.1

Gene Ontology
Bibliography

Related articles in PubMed

Spatiotemporal changes in the distribution of LHFPL5 in mice cochlear hair bundles during development and in the absence of PCDH15.
Mahendrasingam S, Fettiplace R, Alagramam KN, Cross E, Furness DN
PloS one12(10)e0185285(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data show that LHFPL5 is already present in the MET apparatus at P0 but requires PCDH15 at P3 to remain there. Shaft/ankle link localisation suggests it interacts with link proteins other than PCDH15
Title: Spatiotemporal changes in the distribution of LHFPL5 in mice cochlear hair bundles during development and in the absence of PCDH15.

TMHS is an integral component of the hair cell's mechanotransduction machinery that functionally couples PCDH15 to the transduction channel.
Title: TMHS is an integral component of the mechanotransduction machinery of cochlear hair cells.

Hurry-scurry (hscy)-2J, a mutation of Tmhs, causes abnormal splicing from a cryptic splice site within exon 2 that is predicted to produce a functionally null protein lacking 51 amino acids of the wild-type sequence.
Title: Targeted knockout and lacZ reporter expression of the mouse Tmhs deafness gene and characterization of the hscy-2J mutation.

TMHS localizes to the apical membrane of inner ear hair cells during stereocilia formation suggesting a function in hair bundle morphogenesis; a missense mutation underlies deafness in hurry-scurry mice.
Title: A missense mutation in the previously undescribed gene Tmhs underlies deafness in hurry-scurry (hscy) mice.

The following Lhfpl5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lhfpl5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02601	NM_026571.2 Latest version!	Mus musculus lipoma HMGIC fusion partner-like 5 (Lhfpl5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu53705	XM_006524433.4 Latest version!	Mus musculus lipoma HMGIC fusion partner-like 5 (Lhfpl5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu53705	XM_006524433.3	Mus musculus lipoma HMGIC fusion partner-like 5 (Lhfpl5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lhfpl5 ( XM_006524433.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524496.1 Mus musculus lipoma HMGIC fusion partner-like 5 (Lhfpl5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53705
Clone ID Related Accession (Same CDS sequence)	XM_006524433.3 , XM_006524433.4
Accession Version	XM_006524433.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tetraspan membrane protein of hair cell stereocilia isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524433.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGT TGCTGCCAGC CCAGGAGGCC GCCAAGATCT ACCACACCAA CTATGTGCGA 
AACTCGCGCG CTGTGGGAGT GATGTGGGGC ACGCTCACTA TCTGTTTCTC GGTGCTGGTC 
ATGGCCCTCT TTATCCAGCC CTACTGGATA GGCGACAGTG TTAGCACACC ACAGGCTGGT 
TACTTTGGCT TGTTCTCCTA CTGCGTGGGC AACGTGCTGT CGTCGGAACT CATCTGCAAG 
GGGGGCCCTC TGGACTTCTC CTCCATCCCC TCCAGAGCCT TCAAGACTGC CATGTTCTTT 
GTGGCCTTGG CCATGTTCCT TATCATCGGC TCCATCATCT GCTTCAGCCT GTTCTTCGTC 
TGTAATACAG CCACGGTCTA CAAGATTTGC GCCTGGATGC AGCTGGCTGC TGCCACGGGC 
CTAATGATCG GATGTCTAGT CTACCCAGAT GGTTGGGACT CCAGTGAGGT GCGGCGTATG 
TGTGGCGAGC AGACGGGCAA GTACACACTG GGCCACTGCA CCATCCGCTG GGCCTTCATG 
CTGGCCATCC TCAGCATTGG AGATGCACTC ATCCTCTCCT TCCTGGCTTT TGTGTTGGGC 
TATCGGCAGG ACAAGCTCCT CCCGGATGAC TACAAGGCAG ATGGGAATGG TACCTGTCTT 
TCTCCGTGGG AATATCCTTC CCTGGGGCTG TGGGTGGAGA AGTGGCTGCG GTGGTGGCTC 
TCTGAAGCCA AGTGTGTCTG GGCCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524496.1
CDS	633..1382
Translation	MVKLLPAQEAAKIYHTNYVRNSRAVGVMWGTLTICFSVLVMALFIQPYWIGDSVSTPQAGYFGLFSYCVGNVLSSELICKGGPLDFSSIPSRAFKTAMFFVALAMFLIIGSIICFSLFFVCNTATVYKICAWMQLAAATGLMIGCLVYPDGWDSSEVRRMCGEQTGKYTLGHCTIRWAFMLAILSIGDALILSFLAFVLGYRQDKLLPDDYKADGNGTCLSPWEYPSLGLWVEKWLRWWLSEAKCVWAS

Target ORF information:

RefSeq Version	XM_006524433.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lipoma HMGIC fusion partner-like 5 (Lhfpl5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524433.3

ORF Insert Sequence:

ATGGTGAAGT TGCTGCCAGC CCAGGAGGCC GCCAAGATCT ACCACACCAA CTATGTGCGA 
AACTCGCGCG CTGTGGGAGT GATGTGGGGC ACGCTCACTA TCTGTTTCTC GGTGCTGGTC 
ATGGCCCTCT TTATCCAGCC CTACTGGATA GGCGACAGTG TTAGCACACC ACAGGCTGGT 
TACTTTGGCT TGTTCTCCTA CTGCGTGGGC AACGTGCTGT CGTCGGAACT CATCTGCAAG 
GGGGGCCCTC TGGACTTCTC CTCCATCCCC TCCAGAGCCT TCAAGACTGC CATGTTCTTT 
GTGGCCTTGG CCATGTTCCT TATCATCGGC TCCATCATCT GCTTCAGCCT GTTCTTCGTC 
TGTAATACAG CCACGGTCTA CAAGATTTGC GCCTGGATGC AGCTGGCTGC TGCCACGGGC 
CTAATGATCG GATGTCTAGT CTACCCAGAT GGTTGGGACT CCAGTGAGGT GCGGCGTATG 
TGTGGCGAGC AGACGGGCAA GTACACACTG GGCCACTGCA CCATCCGCTG GGCCTTCATG 
CTGGCCATCC TCAGCATTGG AGATGCACTC ATCCTCTCCT TCCTGGCTTT TGTGTTGGGC 
TATCGGCAGG ACAAGCTCCT CCCGGATGAC TACAAGGCAG ATGGGAATGG TACCTGTCTT 
TCTCCGTGGG AATATCCTTC CCTGGGGCTG TGGGTGGAGA AGTGGCTGCG GTGGTGGCTC 
TCTGAAGCCA AGTGTGTCTG GGCCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lhfpl5 ( NM_026571.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080847.2 Mus musculus lipoma HMGIC fusion partner-like 5 (Lhfpl5), mRNA.

Price & Availability↑

CloneID	OMu02601
Clone ID Related Accession (Same CDS sequence)	NM_026571.2
Accession Version	NM_026571.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	LHFPL tetraspan subfamily member 5 protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY721459.1 and BC099483.1. On Nov 15, 2007 this sequence version replaced NM_026571.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC099483.1, AK020389.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849389, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGT TGCTGCCAGC CCAGGAGGCC GCCAAGATCT ACCACACCAA CTATGTGCGA 
AACTCGCGCG CTGTGGGAGT GATGTGGGGC ACGCTCACTA TCTGTTTCTC GGTGCTGGTC 
ATGGCCCTCT TTATCCAGCC CTACTGGATA GGCGACAGTG TTAGCACACC ACAGGCTGGT 
TACTTTGGCT TGTTCTCCTA CTGCGTGGGC AACGTGCTGT CGTCGGAACT CATCTGCAAG 
GGGGGCCCTC TGGACTTCTC CTCCATCCCC TCCAGAGCCT TCAAGACTGC CATGTTCTTT 
GTGGCCTTGG CCATGTTCCT TATCATCGGC TCCATCATCT GCTTCAGCCT GTTCTTCGTC 
TGTAATACAG CCACGGTCTA CAAGATTTGC GCCTGGATGC AGCTGGCTGC TGCCACGGGC 
CTAATGATCG GATGTCTAGT CTACCCAGAT GGTTGGGACT CCAGTGAGGT GCGGCGTATG 
TGTGGCGAGC AGACGGGCAA GTACACACTG GGCCACTGCA CCATCCGCTG GGCCTTCATG 
CTGGCCATCC TCAGCATTGG AGATGCACTC ATCCTCTCCT TCCTGGCTTT TGTGTTGGGC 
TATCGGCAGG ACAAGCTCCT CCCGGATGAC TACAAGGCAG ATGGGAATGA GGAAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080847.2
CDS	283..942
Translation	MVKLLPAQEAAKIYHTNYVRNSRAVGVMWGTLTICFSVLVMALFIQPYWIGDSVSTPQAGYFGLFSYCVGNVLSSELICKGGPLDFSSIPSRAFKTAMFFVALAMFLIIGSIICFSLFFVCNTATVYKICAWMQLAAATGLMIGCLVYPDGWDSSEVRRMCGEQTGKYTLGHCTIRWAFMLAILSIGDALILSFLAFVLGYRQDKLLPDDYKADGNEEV

Target ORF information:

RefSeq Version	NM_026571.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lipoma HMGIC fusion partner-like 5 (Lhfpl5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026571.2

ORF Insert Sequence:

ATGGTGAAGT TGCTGCCAGC CCAGGAGGCC GCCAAGATCT ACCACACCAA CTATGTGCGA 
AACTCGCGCG CTGTGGGAGT GATGTGGGGC ACGCTCACTA TCTGTTTCTC GGTGCTGGTC 
ATGGCCCTCT TTATCCAGCC CTACTGGATA GGCGACAGTG TTAGCACACC ACAGGCTGGT 
TACTTTGGCT TGTTCTCCTA CTGCGTGGGC AACGTGCTGT CGTCGGAACT CATCTGCAAG 
GGGGGCCCTC TGGACTTCTC CTCCATCCCC TCCAGAGCCT TCAAGACTGC CATGTTCTTT 
GTGGCCTTGG CCATGTTCCT TATCATCGGC TCCATCATCT GCTTCAGCCT GTTCTTCGTC 
TGTAATACAG CCACGGTCTA CAAGATTTGC GCCTGGATGC AGCTGGCTGC TGCCACGGGC 
CTAATGATCG GATGTCTAGT CTACCCAGAT GGTTGGGACT CCAGTGAGGT GCGGCGTATG 
TGTGGCGAGC AGACGGGCAA GTACACACTG GGCCACTGCA CCATCCGCTG GGCCTTCATG 
CTGGCCATCC TCAGCATTGG AGATGCACTC ATCCTCTCCT TCCTGGCTTT TGTGTTGGGC 
TATCGGCAGG ACAAGCTCCT CCCGGATGAC TACAAGGCAG ATGGGAATGA GGAAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lhfpl5 ( XM_006524433.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524496.1 Mus musculus lipoma HMGIC fusion partner-like 5 (Lhfpl5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53705
Clone ID Related Accession (Same CDS sequence)	XM_006524433.3 , XM_006524433.4
Accession Version	XM_006524433.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	LHFPL tetraspan subfamily member 5 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524433.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGT TGCTGCCAGC CCAGGAGGCC GCCAAGATCT ACCACACCAA CTATGTGCGA 
AACTCGCGCG CTGTGGGAGT GATGTGGGGC ACGCTCACTA TCTGTTTCTC GGTGCTGGTC 
ATGGCCCTCT TTATCCAGCC CTACTGGATA GGCGACAGTG TTAGCACACC ACAGGCTGGT 
TACTTTGGCT TGTTCTCCTA CTGCGTGGGC AACGTGCTGT CGTCGGAACT CATCTGCAAG 
GGGGGCCCTC TGGACTTCTC CTCCATCCCC TCCAGAGCCT TCAAGACTGC CATGTTCTTT 
GTGGCCTTGG CCATGTTCCT TATCATCGGC TCCATCATCT GCTTCAGCCT GTTCTTCGTC 
TGTAATACAG CCACGGTCTA CAAGATTTGC GCCTGGATGC AGCTGGCTGC TGCCACGGGC 
CTAATGATCG GATGTCTAGT CTACCCAGAT GGTTGGGACT CCAGTGAGGT GCGGCGTATG 
TGTGGCGAGC AGACGGGCAA GTACACACTG GGCCACTGCA CCATCCGCTG GGCCTTCATG 
CTGGCCATCC TCAGCATTGG AGATGCACTC ATCCTCTCCT TCCTGGCTTT TGTGTTGGGC 
TATCGGCAGG ACAAGCTCCT CCCGGATGAC TACAAGGCAG ATGGGAATGG TACCTGTCTT 
TCTCCGTGGG AATATCCTTC CCTGGGGCTG TGGGTGGAGA AGTGGCTGCG GTGGTGGCTC 
TCTGAAGCCA AGTGTGTCTG GGCCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524496.1
CDS	633..1382
Translation	MVKLLPAQEAAKIYHTNYVRNSRAVGVMWGTLTICFSVLVMALFIQPYWIGDSVSTPQAGYFGLFSYCVGNVLSSELICKGGPLDFSSIPSRAFKTAMFFVALAMFLIIGSIICFSLFFVCNTATVYKICAWMQLAAATGLMIGCLVYPDGWDSSEVRRMCGEQTGKYTLGHCTIRWAFMLAILSIGDALILSFLAFVLGYRQDKLLPDDYKADGNGTCLSPWEYPSLGLWVEKWLRWWLSEAKCVWAS

Target ORF information:

RefSeq Version	XM_006524433.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus lipoma HMGIC fusion partner-like 5 (Lhfpl5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524433.4

ORF Insert Sequence:

ATGGTGAAGT TGCTGCCAGC CCAGGAGGCC GCCAAGATCT ACCACACCAA CTATGTGCGA 
AACTCGCGCG CTGTGGGAGT GATGTGGGGC ACGCTCACTA TCTGTTTCTC GGTGCTGGTC 
ATGGCCCTCT TTATCCAGCC CTACTGGATA GGCGACAGTG TTAGCACACC ACAGGCTGGT 
TACTTTGGCT TGTTCTCCTA CTGCGTGGGC AACGTGCTGT CGTCGGAACT CATCTGCAAG 
GGGGGCCCTC TGGACTTCTC CTCCATCCCC TCCAGAGCCT TCAAGACTGC CATGTTCTTT 
GTGGCCTTGG CCATGTTCCT TATCATCGGC TCCATCATCT GCTTCAGCCT GTTCTTCGTC 
TGTAATACAG CCACGGTCTA CAAGATTTGC GCCTGGATGC AGCTGGCTGC TGCCACGGGC 
CTAATGATCG GATGTCTAGT CTACCCAGAT GGTTGGGACT CCAGTGAGGT GCGGCGTATG 
TGTGGCGAGC AGACGGGCAA GTACACACTG GGCCACTGCA CCATCCGCTG GGCCTTCATG 
CTGGCCATCC TCAGCATTGG AGATGCACTC ATCCTCTCCT TCCTGGCTTT TGTGTTGGGC 
TATCGGCAGG ACAAGCTCCT CCCGGATGAC TACAAGGCAG ATGGGAATGG TACCTGTCTT 
TCTCCGTGGG AATATCCTTC CCTGGGGCTG TGGGTGGAGA AGTGGCTGCG GTGGTGGCTC 
TCTGAAGCCA AGTGTGTCTG GGCCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spatiotemporal changes in the distribution of LHFPL5 in mice cochlear hair bundles during development and in the absence of PCDH15.
PloS one12(10)e0185285(2017)
Mahendrasingam S,Fettiplace R,Alagramam KN,Cross E,Furness DN

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Lhfpl5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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