Upf2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Upf2
Entrez Gene ID	326622
Full Name	UPF2 regulator of nonsense transcripts homolog (yeast)
General protein information	Preferred Names UPF2 regulator of nonsense transcripts homolog (yeast) Names regulator of nonsense transcripts 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006497485.3	XP_006497548.1	regulator of nonsense transcripts 2 isoform X1
XM_006497488.4	XP_006497551.1	regulator of nonsense transcripts 2 isoform X4
NM_001081132.1	NP_001074601.1	regulator of nonsense transcripts 2
XM_006497487.1	XP_006497550.1	regulator of nonsense transcripts 2 isoform X3
XM_006497486.1	XP_006497549.1	regulator of nonsense transcripts 2 isoform X2
XM_006497488.3	XP_006497551.1	regulator of nonsense transcripts 2 isoform X4
XM_006497485.4	XP_006497548.1	regulator of nonsense transcripts 2 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UPF2	XP_005214170.1
Homo sapiens (human)	UPF2	NP_542166.1
Rattus norvegicus (Norway rat)	Upf2	NP_001101891.1
Danio rerio (zebrafish)	upf2	XP_697460.6
Drosophila melanogaster (fruit fly)	Upf2	NP_572434.1
Neurospora crassa	NCU05267	XP_961757.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000337	XP_310780.5
Saccharomyces cerevisiae (baker's yeast)	NMD2	NP_011944.2
Eremothecium gossypii	AGOS_AGR364C	NP_987030.2
Pan troglodytes (chimpanzee)	UPF2	XP_507656.3
	UPF2	XP_002805601.1
Gallus gallus (chicken)	UPF2	XP_004937542.1
Kluyveromyces lactis	KLLA0D13156g	XP_453649.1
Magnaporthe oryzae (rice blast fungus)	MGG_06063	XP_003711916.1
Arabidopsis thaliana (thale cress)	AT2G39260	NP_181459.4
Canis lupus familiaris (dog)	UPF2	XP_005617199.1
Mus musculus (house mouse)	Upf2	NP_001074601.1
Schizosaccharomyces pombe (fission yeast)	upf2	NP_593784.1
Xenopus tropicalis (tropical clawed frog)	upf2	NP_001121475.1

Gene Ontology
Bibliography

Related articles in PubMed

Nonsense in the testis: multiple roles for nonsense-mediated decay revealed in male reproduction.
MacDonald CC, Grozdanov PN
Biology of reproduction96(5)939-947(2017 May)

UPF2-Dependent Nonsense-Mediated mRNA Decay Pathway Is Essential for Spermatogenesis by Selectively Eliminating Longer 3'UTR Transcripts.
Bao J, Vitting-Seerup K, Waage J, Tang C, Ge Y, Porse BT, Yan W
PLoS genetics12(5)e1005863(2016 May)

Chromatoid Body Protein TDRD6 Supports Long 3' UTR Triggered Nonsense Mediated mRNA Decay.
Fanourgakis G, Lesche M, Akpinar M, Dahl A, Jessberger R
PLoS genetics12(5)e1005857(2016 May)

Smg6/Est1 licenses embryonic stem cell differentiation via nonsense-mediated mRNA decay.
Li T, Shi Y, Wang P, Guachalla LM, Sun B, Joerss T, Chen YS, Groth M, Krueger A, Platzer M, Yang YG, Rudolph KL, Wang ZQ
The EMBO journal34(12)1630-47(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we report that UPF2-mediated nonsense-mediated mRNA decay (NMD) plays an essential role in male germ cells by eliminating ubiquitous genes-derived, longer 3'UTR transcripts, and that this role is independent of its canonical role in degrading "premature termination codon" (PTC)-containing transcripts in somatic cell lineages
Title: UPF2-Dependent Nonsense-Mediated mRNA Decay Pathway Is Essential for Spermatogenesis by Selectively Eliminating Longer 3'UTR Transcripts.

Upon removal of TDRD6, the association of several mRNAs with UPF1 and UPF2 is disturbed, and the long 3' UTR-stimulated but not the downstream exon-exon junction triggered pathway of NMD is impaired.This function depends on TDRD6-promoted assembly of mRNA and decay enzymes in Chromatoid bodies .
Title: Chromatoid Body Protein TDRD6 Supports Long 3' UTR Triggered Nonsense Mediated mRNA Decay.

UPF2, a nonsense-mediated mRNA decay factor, is required for prepubertal Sertoli cell development and male fertility by ensuring fidelity of the transcriptome.
Title: UPF2, a nonsense-mediated mRNA decay factor, is required for prepubertal Sertoli cell development and male fertility by ensuring fidelity of the transcriptome.

Study find that loss of Upf2 during fetal liver development is incompatible with postnatal life due to failure of terminal differentiation. Moreover, deletion of Upf2 in the adult liver results in hepatosteatosis and disruption of liver homeostasis.
Title: UPF2 is a critical regulator of liver development, function and regeneration.

Hematopoietic-specific deletion of Upf2, a core NMD factor, led to the rapid, complete, and lasting cell-autonomous extinction of all hematopoietic stem and progenitor populations.
Title: NMD is essential for hematopoietic stem and progenitor cells and for eliminating by-products of programmed DNA rearrangements.

The following Upf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Upf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu29165	XM_006497488.4 Latest version!	Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu01752	XM_006497485.4 Latest version!	Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu01752	NM_001081132.1 Latest version!	Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu29163	XM_006497486.1 Latest version!	Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu29164	XM_006497487.1 Latest version!	Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OMu29165	XM_006497488.3	Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu01752	XM_006497485.3	Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Upf2 ( XM_006497488.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497551.1 Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu29165
Clone ID Related Accession (Same CDS sequence)	XM_006497488.4 , XM_006497488.3
Accession Version	XM_006497488.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	regulator of nonsense transcripts 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497488.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTG AGCGTAAAAA GTCAGCAAGT ATGGAAGAAA AAGAATCTTT ACTAAACAAC 
AAGGAAAAAG ACTGCAGTGA GAGGCGGCCA GTGAGCAGCA AGGAGAAACC AAGGGACGAC 
CTCAAGGTCA CTGCCAAGAA GGAGGTCAGT AAGGTCCCAG AAGACAAAAA GAAGAGACTG 
GAAGAAGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGAGGA AGAGAAGGTG 
AAGGCAGAGG AAGAGTTAAA GAAGAAGGAA GAGGAAGAAA AGAAGAAACA GGAAGAGGAA 
GAGAGGAAAA AGCAGGAGGA GCAGGCCAAG CGGCAGCAGG AAGAAGCGGC TGCTCAGCTG 
AAAGAGAAAG AAGAATCTCT TCAGCTTCAT CAGGAGGCCT GGGAACGCCA TCAATTACGA 
AAGGAACTTC GTAGCAAAAA CCAAAATGCT CCAGACAACC GACCAGAGGA AAATTTCTTT 
AGCCGCCTAG ATTCGAGCTT AAAGAAAAAT ACCGCTTTTG TCAAGAAACT AAAAACAATT 
ACAGAACAAC AGAGAGACTC CTTGTCTCAT GATTTTAATG GCCTGAATTT AAGCAAATAT 
ATTGCAGAAG CCGTAGCTTC CATTGTGGAG GCAAAATTAA AACTCTCTGA CGTGAACTGT 
GCTGCGCACC TCTGCTCTCT CTTTCACCAG CGGTACTCTG ACTTTGCGCC CTCTCTTCTT 
CAGGTGTGGA AAAAACATTT CGAAGCGAGG AAAGAGGAGA AAACGCCTAA CATTACCAAG 
CTAAGAACTG ACTTGCGTTT CATTGCAGAA TTGACAATAG TGGGAATTTT CACTGACAAG 
GAAGGTCTTT CCTTAATCTA CGAACAGCTA AAAAGTATTA TTAATGCTGA TCGAGAATCC 
CACACTCATG TTTCTGTGGT GATTAGTTTC TGTCGCCATT GTGGAGATGA CATTGCTGGA 
CTTGTACCGA GGAAAGTAAA AAGTGCTGCG GAGAAGTTTA ATTTGAGTTT TCCTCCTAGT 
GAGATCATTA GTCCGGAGAA GCAGCAGCCT TTCCAGAATC TTTTAAAAGA GTACTTTACG 
TCTTTGACCA AACACCTGAA AAGGGACCAC AGGGAGCTGC AGAACACTGA GAGGCAAAAC 
AGGCGTATTC TGCACTCTAA AGGCGAGCTA AGTGAGGACA GGCACAAGCA ATATGAGGAG 
TTTGCAATGT CTTACCAGAA GCTGCTGGCG AACTCTCAGT CCTTAGCGGA CCTTTTGGAT 
GAAAATATGC CCGATCTTCC TCAGGACAAA CCCACACCAG AAGAGCATGG GCCCGGAATC 
GACATATTTA CACCCGGCAA ACCTGGAGAG TATGACTTAG AAGGTGGCAT TTGGGAAGAT 
GAAGACGCTC GGAATTTTTA TGAAAACCTC ATCGATTTGA AAGCATTTGT TCCAGCCATC 
TTGTTCAAAG ACAATGAAAA AAGTCAGAAC AAAGATTCTA ACAAAGATGA TTCCAAAGAG 
GCAAAAGAAC CAAAGGATAA TAAGGAGGCA TCAAGTCCTG ATGATTTGGA ACTTGAGTTG 
GAGAATTTAG AAATTAATGA TGATACATTG GAGTTAGAAG GTGCAGATGA AGCTGAAGAT 
CTTACAAAGA AACTTCTTGA TGAACAAGAA CAAGAAGATG AAGAAGCCAG CACTGGATCT 
CATCTCAAAC TCATAGTAGA TGCTTTCCTC CAGCAGCTGC CCAACTGTGT CAACCGAGAC 
CTAATCGACA AGGCAGCCAT GGATTTTTGC ATGAACATGA ACACAAAGGC AAACAGGAAG 
AAGTTGGTGC GGGCACTCTT CATAGTTCCT AGGCAGAGGT TGGATTTGCT ACCATTTTAT 
GCAAGATTGG TTGCTACCTT GCACCCCTGC ATGTCTGATG TAGCAGAGGA TCTTTGTTCC 
ATGCTGAGGG GGGATTTCAG ATTTCATGTC CAATATATTG CCACAGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497551.1
CDS	126..2096
Translation	MPAERKKSASMEEKESLLNNKEKDCSERRPVSSKEKPRDDLKVTAKKEVSKVPEDKKKRLEEDKRKKEDKERKKKEEEKVKAEEELKKKEEEEKKKQEEEERKKQEEQAKRQQEEAAAQLKEKEESLQLHQEAWERHQLRKELRSKNQNAPDNRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKLSDVNCAAHLCSLFHQRYSDFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDKEGLSLIYEQLKSIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSQNKDSNKDDSKEAKEPKDNKEASSPDDLELELENLEINDDTLELEGADEAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLRGDFRFHVQYIATV

Target ORF information:

RefSeq Version	XM_006497488.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497488.3

ORF Insert Sequence:

ATGCCAGCTG AGCGTAAAAA GTCAGCAAGT ATGGAAGAAA AAGAATCTTT ACTAAACAAC 
AAGGAAAAAG ACTGCAGTGA GAGGCGGCCA GTGAGCAGCA AGGAGAAACC AAGGGACGAC 
CTCAAGGTCA CTGCCAAGAA GGAGGTCAGT AAGGTCCCAG AAGACAAAAA GAAGAGACTG 
GAAGAAGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGAGGA AGAGAAGGTG 
AAGGCAGAGG AAGAGTTAAA GAAGAAGGAA GAGGAAGAAA AGAAGAAACA GGAAGAGGAA 
GAGAGGAAAA AGCAGGAGGA GCAGGCCAAG CGGCAGCAGG AAGAAGCGGC TGCTCAGCTG 
AAAGAGAAAG AAGAATCTCT TCAGCTTCAT CAGGAGGCCT GGGAACGCCA TCAATTACGA 
AAGGAACTTC GTAGCAAAAA CCAAAATGCT CCAGACAACC GACCAGAGGA AAATTTCTTT 
AGCCGCCTAG ATTCGAGCTT AAAGAAAAAT ACCGCTTTTG TCAAGAAACT AAAAACAATT 
ACAGAACAAC AGAGAGACTC CTTGTCTCAT GATTTTAATG GCCTGAATTT AAGCAAATAT 
ATTGCAGAAG CCGTAGCTTC CATTGTGGAG GCAAAATTAA AACTCTCTGA CGTGAACTGT 
GCTGCGCACC TCTGCTCTCT CTTTCACCAG CGGTACTCTG ACTTTGCGCC CTCTCTTCTT 
CAGGTGTGGA AAAAACATTT CGAAGCGAGG AAAGAGGAGA AAACGCCTAA CATTACCAAG 
CTAAGAACTG ACTTGCGTTT CATTGCAGAA TTGACAATAG TGGGAATTTT CACTGACAAG 
GAAGGTCTTT CCTTAATCTA CGAACAGCTA AAAAGTATTA TTAATGCTGA TCGAGAATCC 
CACACTCATG TTTCTGTGGT GATTAGTTTC TGTCGCCATT GTGGAGATGA CATTGCTGGA 
CTTGTACCGA GGAAAGTAAA AAGTGCTGCG GAGAAGTTTA ATTTGAGTTT TCCTCCTAGT 
GAGATCATTA GTCCGGAGAA GCAGCAGCCT TTCCAGAATC TTTTAAAAGA GTACTTTACG 
TCTTTGACCA AACACCTGAA AAGGGACCAC AGGGAGCTGC AGAACACTGA GAGGCAAAAC 
AGGCGTATTC TGCACTCTAA AGGCGAGCTA AGTGAGGACA GGCACAAGCA ATATGAGGAG 
TTTGCAATGT CTTACCAGAA GCTGCTGGCG AACTCTCAGT CCTTAGCGGA CCTTTTGGAT 
GAAAATATGC CCGATCTTCC TCAGGACAAA CCCACACCAG AAGAGCATGG GCCCGGAATC 
GACATATTTA CACCCGGCAA ACCTGGAGAG TATGACTTAG AAGGTGGCAT TTGGGAAGAT 
GAAGACGCTC GGAATTTTTA TGAAAACCTC ATCGATTTGA AAGCATTTGT TCCAGCCATC 
TTGTTCAAAG ACAATGAAAA AAGTCAGAAC AAAGATTCTA ACAAAGATGA TTCCAAAGAG 
GCAAAAGAAC CAAAGGATAA TAAGGAGGCA TCAAGTCCTG ATGATTTGGA ACTTGAGTTG 
GAGAATTTAG AAATTAATGA TGATACATTG GAGTTAGAAG GTGCAGATGA AGCTGAAGAT 
CTTACAAAGA AACTTCTTGA TGAACAAGAA CAAGAAGATG AAGAAGCCAG CACTGGATCT 
CATCTCAAAC TCATAGTAGA TGCTTTCCTC CAGCAGCTGC CCAACTGTGT CAACCGAGAC 
CTAATCGACA AGGCAGCCAT GGATTTTTGC ATGAACATGA ACACAAAGGC AAACAGGAAG 
AAGTTGGTGC GGGCACTCTT CATAGTTCCT AGGCAGAGGT TGGATTTGCT ACCATTTTAT 
GCAAGATTGG TTGCTACCTT GCACCCCTGC ATGTCTGATG TAGCAGAGGA TCTTTGTTCC 
ATGCTGAGGG GGGATTTCAG ATTTCATGTC CAATATATTG CCACAGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Upf2 ( XM_006497488.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497551.1 Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu29165
Clone ID Related Accession (Same CDS sequence)	XM_006497488.4 , XM_006497488.3
Accession Version	XM_006497488.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	regulator of nonsense transcripts 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497488.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTG AGCGTAAAAA GTCAGCAAGT ATGGAAGAAA AAGAATCTTT ACTAAACAAC 
AAGGAAAAAG ACTGCAGTGA GAGGCGGCCA GTGAGCAGCA AGGAGAAACC AAGGGACGAC 
CTCAAGGTCA CTGCCAAGAA GGAGGTCAGT AAGGTCCCAG AAGACAAAAA GAAGAGACTG 
GAAGAAGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGAGGA AGAGAAGGTG 
AAGGCAGAGG AAGAGTTAAA GAAGAAGGAA GAGGAAGAAA AGAAGAAACA GGAAGAGGAA 
GAGAGGAAAA AGCAGGAGGA GCAGGCCAAG CGGCAGCAGG AAGAAGCGGC TGCTCAGCTG 
AAAGAGAAAG AAGAATCTCT TCAGCTTCAT CAGGAGGCCT GGGAACGCCA TCAATTACGA 
AAGGAACTTC GTAGCAAAAA CCAAAATGCT CCAGACAACC GACCAGAGGA AAATTTCTTT 
AGCCGCCTAG ATTCGAGCTT AAAGAAAAAT ACCGCTTTTG TCAAGAAACT AAAAACAATT 
ACAGAACAAC AGAGAGACTC CTTGTCTCAT GATTTTAATG GCCTGAATTT AAGCAAATAT 
ATTGCAGAAG CCGTAGCTTC CATTGTGGAG GCAAAATTAA AACTCTCTGA CGTGAACTGT 
GCTGCGCACC TCTGCTCTCT CTTTCACCAG CGGTACTCTG ACTTTGCGCC CTCTCTTCTT 
CAGGTGTGGA AAAAACATTT CGAAGCGAGG AAAGAGGAGA AAACGCCTAA CATTACCAAG 
CTAAGAACTG ACTTGCGTTT CATTGCAGAA TTGACAATAG TGGGAATTTT CACTGACAAG 
GAAGGTCTTT CCTTAATCTA CGAACAGCTA AAAAGTATTA TTAATGCTGA TCGAGAATCC 
CACACTCATG TTTCTGTGGT GATTAGTTTC TGTCGCCATT GTGGAGATGA CATTGCTGGA 
CTTGTACCGA GGAAAGTAAA AAGTGCTGCG GAGAAGTTTA ATTTGAGTTT TCCTCCTAGT 
GAGATCATTA GTCCGGAGAA GCAGCAGCCT TTCCAGAATC TTTTAAAAGA GTACTTTACG 
TCTTTGACCA AACACCTGAA AAGGGACCAC AGGGAGCTGC AGAACACTGA GAGGCAAAAC 
AGGCGTATTC TGCACTCTAA AGGCGAGCTA AGTGAGGACA GGCACAAGCA ATATGAGGAG 
TTTGCAATGT CTTACCAGAA GCTGCTGGCG AACTCTCAGT CCTTAGCGGA CCTTTTGGAT 
GAAAATATGC CCGATCTTCC TCAGGACAAA CCCACACCAG AAGAGCATGG GCCCGGAATC 
GACATATTTA CACCCGGCAA ACCTGGAGAG TATGACTTAG AAGGTGGCAT TTGGGAAGAT 
GAAGACGCTC GGAATTTTTA TGAAAACCTC ATCGATTTGA AAGCATTTGT TCCAGCCATC 
TTGTTCAAAG ACAATGAAAA AAGTCAGAAC AAAGATTCTA ACAAAGATGA TTCCAAAGAG 
GCAAAAGAAC CAAAGGATAA TAAGGAGGCA TCAAGTCCTG ATGATTTGGA ACTTGAGTTG 
GAGAATTTAG AAATTAATGA TGATACATTG GAGTTAGAAG GTGCAGATGA AGCTGAAGAT 
CTTACAAAGA AACTTCTTGA TGAACAAGAA CAAGAAGATG AAGAAGCCAG CACTGGATCT 
CATCTCAAAC TCATAGTAGA TGCTTTCCTC CAGCAGCTGC CCAACTGTGT CAACCGAGAC 
CTAATCGACA AGGCAGCCAT GGATTTTTGC ATGAACATGA ACACAAAGGC AAACAGGAAG 
AAGTTGGTGC GGGCACTCTT CATAGTTCCT AGGCAGAGGT TGGATTTGCT ACCATTTTAT 
GCAAGATTGG TTGCTACCTT GCACCCCTGC ATGTCTGATG TAGCAGAGGA TCTTTGTTCC 
ATGCTGAGGG GGGATTTCAG ATTTCATGTC CAATATATTG CCACAGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497551.1
CDS	126..2096
Translation	MPAERKKSASMEEKESLLNNKEKDCSERRPVSSKEKPRDDLKVTAKKEVSKVPEDKKKRLEEDKRKKEDKERKKKEEEKVKAEEELKKKEEEEKKKQEEEERKKQEEQAKRQQEEAAAQLKEKEESLQLHQEAWERHQLRKELRSKNQNAPDNRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKLSDVNCAAHLCSLFHQRYSDFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDKEGLSLIYEQLKSIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSQNKDSNKDDSKEAKEPKDNKEASSPDDLELELENLEINDDTLELEGADEAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLRGDFRFHVQYIATV

Target ORF information:

RefSeq Version	XM_006497488.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497488.4

ORF Insert Sequence:

ATGCCAGCTG AGCGTAAAAA GTCAGCAAGT ATGGAAGAAA AAGAATCTTT ACTAAACAAC 
AAGGAAAAAG ACTGCAGTGA GAGGCGGCCA GTGAGCAGCA AGGAGAAACC AAGGGACGAC 
CTCAAGGTCA CTGCCAAGAA GGAGGTCAGT AAGGTCCCAG AAGACAAAAA GAAGAGACTG 
GAAGAAGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGAGGA AGAGAAGGTG 
AAGGCAGAGG AAGAGTTAAA GAAGAAGGAA GAGGAAGAAA AGAAGAAACA GGAAGAGGAA 
GAGAGGAAAA AGCAGGAGGA GCAGGCCAAG CGGCAGCAGG AAGAAGCGGC TGCTCAGCTG 
AAAGAGAAAG AAGAATCTCT TCAGCTTCAT CAGGAGGCCT GGGAACGCCA TCAATTACGA 
AAGGAACTTC GTAGCAAAAA CCAAAATGCT CCAGACAACC GACCAGAGGA AAATTTCTTT 
AGCCGCCTAG ATTCGAGCTT AAAGAAAAAT ACCGCTTTTG TCAAGAAACT AAAAACAATT 
ACAGAACAAC AGAGAGACTC CTTGTCTCAT GATTTTAATG GCCTGAATTT AAGCAAATAT 
ATTGCAGAAG CCGTAGCTTC CATTGTGGAG GCAAAATTAA AACTCTCTGA CGTGAACTGT 
GCTGCGCACC TCTGCTCTCT CTTTCACCAG CGGTACTCTG ACTTTGCGCC CTCTCTTCTT 
CAGGTGTGGA AAAAACATTT CGAAGCGAGG AAAGAGGAGA AAACGCCTAA CATTACCAAG 
CTAAGAACTG ACTTGCGTTT CATTGCAGAA TTGACAATAG TGGGAATTTT CACTGACAAG 
GAAGGTCTTT CCTTAATCTA CGAACAGCTA AAAAGTATTA TTAATGCTGA TCGAGAATCC 
CACACTCATG TTTCTGTGGT GATTAGTTTC TGTCGCCATT GTGGAGATGA CATTGCTGGA 
CTTGTACCGA GGAAAGTAAA AAGTGCTGCG GAGAAGTTTA ATTTGAGTTT TCCTCCTAGT 
GAGATCATTA GTCCGGAGAA GCAGCAGCCT TTCCAGAATC TTTTAAAAGA GTACTTTACG 
TCTTTGACCA AACACCTGAA AAGGGACCAC AGGGAGCTGC AGAACACTGA GAGGCAAAAC 
AGGCGTATTC TGCACTCTAA AGGCGAGCTA AGTGAGGACA GGCACAAGCA ATATGAGGAG 
TTTGCAATGT CTTACCAGAA GCTGCTGGCG AACTCTCAGT CCTTAGCGGA CCTTTTGGAT 
GAAAATATGC CCGATCTTCC TCAGGACAAA CCCACACCAG AAGAGCATGG GCCCGGAATC 
GACATATTTA CACCCGGCAA ACCTGGAGAG TATGACTTAG AAGGTGGCAT TTGGGAAGAT 
GAAGACGCTC GGAATTTTTA TGAAAACCTC ATCGATTTGA AAGCATTTGT TCCAGCCATC 
TTGTTCAAAG ACAATGAAAA AAGTCAGAAC AAAGATTCTA ACAAAGATGA TTCCAAAGAG 
GCAAAAGAAC CAAAGGATAA TAAGGAGGCA TCAAGTCCTG ATGATTTGGA ACTTGAGTTG 
GAGAATTTAG AAATTAATGA TGATACATTG GAGTTAGAAG GTGCAGATGA AGCTGAAGAT 
CTTACAAAGA AACTTCTTGA TGAACAAGAA CAAGAAGATG AAGAAGCCAG CACTGGATCT 
CATCTCAAAC TCATAGTAGA TGCTTTCCTC CAGCAGCTGC CCAACTGTGT CAACCGAGAC 
CTAATCGACA AGGCAGCCAT GGATTTTTGC ATGAACATGA ACACAAAGGC AAACAGGAAG 
AAGTTGGTGC GGGCACTCTT CATAGTTCCT AGGCAGAGGT TGGATTTGCT ACCATTTTAT 
GCAAGATTGG TTGCTACCTT GCACCCCTGC ATGTCTGATG TAGCAGAGGA TCTTTGTTCC 
ATGCTGAGGG GGGATTTCAG ATTTCATGTC CAATATATTG CCACAGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Upf2 ( XM_006497485.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497548.1 Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X1, mRNA.

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CloneID	OMu01752
Clone ID Related Accession (Same CDS sequence)	XM_006497485.3 , NM_001081132.1 , XM_006497485.4
Accession Version	XM_006497485.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	regulator of nonsense transcripts 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497485.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTG AGCGTAAAAA GTCAGCAAGT ATGGAAGAAA AAGAATCTTT ACTAAACAAC 
AAGGAAAAAG ACTGCAGTGA GAGGCGGCCA GTGAGCAGCA AGGAGAAACC AAGGGACGAC 
CTCAAGGTCA CTGCCAAGAA GGAGGTCAGT AAGGTCCCAG AAGACAAAAA GAAGAGACTG 
GAAGAAGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGAGGA AGAGAAGGTG 
AAGGCAGAGG AAGAGTTAAA GAAGAAGGAA GAGGAAGAAA AGAAGAAACA GGAAGAGGAA 
GAGAGGAAAA AGCAGGAGGA GCAGGCCAAG CGGCAGCAGG AAGAAGCGGC TGCTCAGCTG 
AAAGAGAAAG AAGAATCTCT TCAGCTTCAT CAGGAGGCCT GGGAACGCCA TCAATTACGA 
AAGGAACTTC GTAGCAAAAA CCAAAATGCT CCAGACAACC GACCAGAGGA AAATTTCTTT 
AGCCGCCTAG ATTCGAGCTT AAAGAAAAAT ACCGCTTTTG TCAAGAAACT AAAAACAATT 
ACAGAACAAC AGAGAGACTC CTTGTCTCAT GATTTTAATG GCCTGAATTT AAGCAAATAT 
ATTGCAGAAG CCGTAGCTTC CATTGTGGAG GCAAAATTAA AACTCTCTGA CGTGAACTGT 
GCTGCGCACC TCTGCTCTCT CTTTCACCAG CGGTACTCTG ACTTTGCGCC CTCTCTTCTT 
CAGGTGTGGA AAAAACATTT CGAAGCGAGG AAAGAGGAGA AAACGCCTAA CATTACCAAG 
CTAAGAACTG ACTTGCGTTT CATTGCAGAA TTGACAATAG TGGGAATTTT CACTGACAAG 
GAAGGTCTTT CCTTAATCTA CGAACAGCTA AAAAGTATTA TTAATGCTGA TCGAGAATCC 
CACACTCATG TTTCTGTGGT GATTAGTTTC TGTCGCCATT GTGGAGATGA CATTGCTGGA 
CTTGTACCGA GGAAAGTAAA AAGTGCTGCG GAGAAGTTTA ATTTGAGTTT TCCTCCTAGT 
GAGATCATTA GTCCGGAGAA GCAGCAGCCT TTCCAGAATC TTTTAAAAGA GTACTTTACG 
TCTTTGACCA AACACCTGAA AAGGGACCAC AGGGAGCTGC AGAACACTGA GAGGCAAAAC 
AGGCGTATTC TGCACTCTAA AGGCGAGCTA AGTGAGGACA GGCACAAGCA ATATGAGGAG 
TTTGCAATGT CTTACCAGAA GCTGCTGGCG AACTCTCAGT CCTTAGCGGA CCTTTTGGAT 
GAAAATATGC CCGATCTTCC TCAGGACAAA CCCACACCAG AAGAGCATGG GCCCGGAATC 
GACATATTTA CACCCGGCAA ACCTGGAGAG TATGACTTAG AAGGTGGCAT TTGGGAAGAT 
GAAGACGCTC GGAATTTTTA TGAAAACCTC ATCGATTTGA AAGCATTTGT TCCAGCCATC 
TTGTTCAAAG ACAATGAAAA AAGTCAGAAC AAAGATTCTA ACAAAGATGA TTCCAAAGAG 
GCAAAAGAAC CAAAGGATAA TAAGGAGGCA TCAAGTCCTG ATGATTTGGA ACTTGAGTTG 
GAGAATTTAG AAATTAATGA TGATACATTG GAGTTAGAAG GTGCAGATGA AGCTGAAGAT 
CTTACAAAGA AACTTCTTGA TGAACAAGAA CAAGAAGATG AAGAAGCCAG CACTGGATCT 
CATCTCAAAC TCATAGTAGA TGCTTTCCTC CAGCAGCTGC CCAACTGTGT CAACCGAGAC 
CTAATCGACA AGGCAGCCAT GGATTTTTGC ATGAACATGA ACACAAAGGC AAACAGGAAG 
AAGTTGGTGC GGGCACTCTT CATAGTTCCT AGGCAGAGGT TGGATTTGCT ACCATTTTAT 
GCAAGATTGG TTGCTACCTT GCACCCCTGC ATGTCTGATG TAGCAGAGGA TCTTTGTTCC 
ATGCTGAGGG GGGATTTCAG ATTTCATGTT CGAAAAAAGG ACCAGATCAA CATTGAAACA 
AAGAACAAAA CTGTCCGCTT TATTGGAGAG CTAACCAAGT TTAAGATGTT CACCAAAAAT 
GATACACTGC ATTGTCTAAA GATGCTTCTG TCAGATTTCT CTCATCATCA TATTGAAATG 
GCATGCACTC TACTGGAGAC CTGCGGAAGA TTTCTCTTCA GATCTCCAGA ATCACACCTG 
AGGACCAGTG TGCTTTTGGA GCAAATGATG AGAAAGAAAC AAGCAATGCA TCTTGATGCA 
CGATATGTTA CAATGGTAGA AAATGCATAT TATTACTGCA ACCCACCGCC CGCCGAAAAG 
ACCGTGAGAA AGAAACGCCC TCCTCTTCAG GAGTATGTCC GCAAGCTTCT GTACAAGGAT 
CTCTCTAAGG TCACGACGGA AAAGGTTTTG AGACAGATGC GAAAGTTGCC CTGGCAGGAC 
CAAGAAGTAA AAGACTATGT TATTTGTTGT ATGATAAACA TCTGGAACGT GAAATACAAC 
AGTATTCACT GTGTAGCCAA CCTCCTAGCA GGGCTAGTTC TCTACCAGGA GGATGTTGGG 
ATCCATGTTG TGGATGGCGT ACTCGAAGAT ATTCGATTGG GAATGGAGGT TAATCAACCT 
AAATTTAACC AGAGGCGTAT CAGTAGTGCC AAGTTCTTAG GAGAACTTTA TAATTACAGA 
ATGGTCGAAT CAGCTGTTAT TTTCAGAACT CTTTATTCTT TTACTTCATT TGGTGTCAAC 
CCTGATGGGT CTCCAAGTTC CCTGGACCCT CCTGAGCATC TCTTTAGAAT TAGACTAGTA 
TGCACTATTT TGGATACCTG TGGCCAGTAC TTTGACAGAG GTTCCAGTAA ACGAAAACTT 
GACTGCTTCC TTGTATATTT TCAGCGTTAT GTTTGGTGGA AGAAAAGTTT GGAGGTTTGG 
ACAAAAGACC ATCCATTTCC TATTGATATA GATTACATGA TCAGTGATAC ACTAGAACTG 
CTAAGACCAA AGATCAAACT CTGTAATTCT CTGGAAGAAT CCATCAGGCA GGTACAAGAC 
TTGGAACGAG AATTCTTAAT AAAACTAGGC CTAGTAAATG ACAAAGAGTC CAAAGATTCT 
ATGACAGAAG GGGAAAATCT TGAAGAGGAT GAAGAAGAAG AAGAAGGAGG AGCTGAGACA 
GAGGAACAGT CTGGAAATGA AAGTGAAGTG AACGAGCCAG AAGAAGAAGA GGGTTCTGAG 
GAGGAGGAGG AGGGAGAGGA GGAGGAAGAG GAGAATACAG ATTACCTCAC AGATTCCAAC 
AAGGAGAACG AGACTGATGA AGAAAATGCT GAGGTGATGA TTAAGGGAGG CGGACTGAAG 
CATGTTCCTT GTGTAGAAGA TGAGGACTTC ATTCAAGCTC TGGACAAAAT GATGCTAGAA 
AACCTTCAGC AGCGAAGTGG TGAGTCTGTT AAAGTGCACC AGCTTGATGT TGCTATCCCT 
TTGCATCTCA AAAGCCAGCT GAGGAAAGGG CCCCCGCTTG GCGGTGGGGA AGGAGAGACG 
GAGTCTGCAG ACACGATGCC ATTTGTCATG TTAACAAGAA AAGGCAATAA ACAGCAGTTT 
AAGATTCTCA ATGTGCCCAT GTCATCCCAA CTTGCTGCAA ATCATTGGAA CCAGCAACAA 
GCAGAACAAG AGGAGAGGAT GAGGATGAAA AAGCTCACCT TAGACATCAA TGAACGACAA 
GAGCAAGAAG ATTATCAAGA AATGTTGCAG TCTCTTGCAC AGCGCCCAGC TCCAGCAAAC 
ACTAATCGTG AGCGGCGGCC TCGTTACCAG CATCCAAAGG GAGCACCTAA TGCAGATCTA 
ATCTTTAAGA CTGGTGGGAG GAGACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497548.1
CDS	318..4127
Translation	MPAERKKSASMEEKESLLNNKEKDCSERRPVSSKEKPRDDLKVTAKKEVSKVPEDKKKRLEEDKRKKEDKERKKKEEEKVKAEEELKKKEEEEKKKQEEEERKKQEEQAKRQQEEAAAQLKEKEESLQLHQEAWERHQLRKELRSKNQNAPDNRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKLSDVNCAAHLCSLFHQRYSDFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDKEGLSLIYEQLKSIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSQNKDSNKDDSKEAKEPKDNKEASSPDDLELELENLEINDDTLELEGADEAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVRKKRPPLQEYVRKLLYKDLSKVTTEKVLRQMRKLPWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGSPSSLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHPFPIDIDYMISDTLELLRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKESKDSMTEGENLEEDEEEEEGGAETEEQSGNESEVNEPEEEEGSEEEEEGEEEEEENTDYLTDSNKENETDEENAEVMIKGGGLKHVPCVEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLKSQLRKGPPLGGGEGETESADTMPFVMLTRKGNKQQFKILNVPMSSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR

Target ORF information:

RefSeq Version	XM_006497485.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497485.4

ORF Insert Sequence:

ATGCCAGCTG AGCGTAAAAA GTCAGCAAGT ATGGAAGAAA AAGAATCTTT ACTAAACAAC 
AAGGAAAAAG ACTGCAGTGA GAGGCGGCCA GTGAGCAGCA AGGAGAAACC AAGGGACGAC 
CTCAAGGTCA CTGCCAAGAA GGAGGTCAGT AAGGTCCCAG AAGACAAAAA GAAGAGACTG 
GAAGAAGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGAGGA AGAGAAGGTG 
AAGGCAGAGG AAGAGTTAAA GAAGAAGGAA GAGGAAGAAA AGAAGAAACA GGAAGAGGAA 
GAGAGGAAAA AGCAGGAGGA GCAGGCCAAG CGGCAGCAGG AAGAAGCGGC TGCTCAGCTG 
AAAGAGAAAG AAGAATCTCT TCAGCTTCAT CAGGAGGCCT GGGAACGCCA TCAATTACGA 
AAGGAACTTC GTAGCAAAAA CCAAAATGCT CCAGACAACC GACCAGAGGA AAATTTCTTT 
AGCCGCCTAG ATTCGAGCTT AAAGAAAAAT ACCGCTTTTG TCAAGAAACT AAAAACAATT 
ACAGAACAAC AGAGAGACTC CTTGTCTCAT GATTTTAATG GCCTGAATTT AAGCAAATAT 
ATTGCAGAAG CCGTAGCTTC CATTGTGGAG GCAAAATTAA AACTCTCTGA CGTGAACTGT 
GCTGCGCACC TCTGCTCTCT CTTTCACCAG CGGTACTCTG ACTTTGCGCC CTCTCTTCTT 
CAGGTGTGGA AAAAACATTT CGAAGCGAGG AAAGAGGAGA AAACGCCTAA CATTACCAAG 
CTAAGAACTG ACTTGCGTTT CATTGCAGAA TTGACAATAG TGGGAATTTT CACTGACAAG 
GAAGGTCTTT CCTTAATCTA CGAACAGCTA AAAAGTATTA TTAATGCTGA TCGAGAATCC 
CACACTCATG TTTCTGTGGT GATTAGTTTC TGTCGCCATT GTGGAGATGA CATTGCTGGA 
CTTGTACCGA GGAAAGTAAA AAGTGCTGCG GAGAAGTTTA ATTTGAGTTT TCCTCCTAGT 
GAGATCATTA GTCCGGAGAA GCAGCAGCCT TTCCAGAATC TTTTAAAAGA GTACTTTACG 
TCTTTGACCA AACACCTGAA AAGGGACCAC AGGGAGCTGC AGAACACTGA GAGGCAAAAC 
AGGCGTATTC TGCACTCTAA AGGCGAGCTA AGTGAGGACA GGCACAAGCA ATATGAGGAG 
TTTGCAATGT CTTACCAGAA GCTGCTGGCG AACTCTCAGT CCTTAGCGGA CCTTTTGGAT 
GAAAATATGC CCGATCTTCC TCAGGACAAA CCCACACCAG AAGAGCATGG GCCCGGAATC 
GACATATTTA CACCCGGCAA ACCTGGAGAG TATGACTTAG AAGGTGGCAT TTGGGAAGAT 
GAAGACGCTC GGAATTTTTA TGAAAACCTC ATCGATTTGA AAGCATTTGT TCCAGCCATC 
TTGTTCAAAG ACAATGAAAA AAGTCAGAAC AAAGATTCTA ACAAAGATGA TTCCAAAGAG 
GCAAAAGAAC CAAAGGATAA TAAGGAGGCA TCAAGTCCTG ATGATTTGGA ACTTGAGTTG 
GAGAATTTAG AAATTAATGA TGATACATTG GAGTTAGAAG GTGCAGATGA AGCTGAAGAT 
CTTACAAAGA AACTTCTTGA TGAACAAGAA CAAGAAGATG AAGAAGCCAG CACTGGATCT 
CATCTCAAAC TCATAGTAGA TGCTTTCCTC CAGCAGCTGC CCAACTGTGT CAACCGAGAC 
CTAATCGACA AGGCAGCCAT GGATTTTTGC ATGAACATGA ACACAAAGGC AAACAGGAAG 
AAGTTGGTGC GGGCACTCTT CATAGTTCCT AGGCAGAGGT TGGATTTGCT ACCATTTTAT 
GCAAGATTGG TTGCTACCTT GCACCCCTGC ATGTCTGATG TAGCAGAGGA TCTTTGTTCC 
ATGCTGAGGG GGGATTTCAG ATTTCATGTT CGAAAAAAGG ACCAGATCAA CATTGAAACA 
AAGAACAAAA CTGTCCGCTT TATTGGAGAG CTAACCAAGT TTAAGATGTT CACCAAAAAT 
GATACACTGC ATTGTCTAAA GATGCTTCTG TCAGATTTCT CTCATCATCA TATTGAAATG 
GCATGCACTC TACTGGAGAC CTGCGGAAGA TTTCTCTTCA GATCTCCAGA ATCACACCTG 
AGGACCAGTG TGCTTTTGGA GCAAATGATG AGAAAGAAAC AAGCAATGCA TCTTGATGCA 
CGATATGTTA CAATGGTAGA AAATGCATAT TATTACTGCA ACCCACCGCC CGCCGAAAAG 
ACCGTGAGAA AGAAACGCCC TCCTCTTCAG GAGTATGTCC GCAAGCTTCT GTACAAGGAT 
CTCTCTAAGG TCACGACGGA AAAGGTTTTG AGACAGATGC GAAAGTTGCC CTGGCAGGAC 
CAAGAAGTAA AAGACTATGT TATTTGTTGT ATGATAAACA TCTGGAACGT GAAATACAAC 
AGTATTCACT GTGTAGCCAA CCTCCTAGCA GGGCTAGTTC TCTACCAGGA GGATGTTGGG 
ATCCATGTTG TGGATGGCGT ACTCGAAGAT ATTCGATTGG GAATGGAGGT TAATCAACCT 
AAATTTAACC AGAGGCGTAT CAGTAGTGCC AAGTTCTTAG GAGAACTTTA TAATTACAGA 
ATGGTCGAAT CAGCTGTTAT TTTCAGAACT CTTTATTCTT TTACTTCATT TGGTGTCAAC 
CCTGATGGGT CTCCAAGTTC CCTGGACCCT CCTGAGCATC TCTTTAGAAT TAGACTAGTA 
TGCACTATTT TGGATACCTG TGGCCAGTAC TTTGACAGAG GTTCCAGTAA ACGAAAACTT 
GACTGCTTCC TTGTATATTT TCAGCGTTAT GTTTGGTGGA AGAAAAGTTT GGAGGTTTGG 
ACAAAAGACC ATCCATTTCC TATTGATATA GATTACATGA TCAGTGATAC ACTAGAACTG 
CTAAGACCAA AGATCAAACT CTGTAATTCT CTGGAAGAAT CCATCAGGCA GGTACAAGAC 
TTGGAACGAG AATTCTTAAT AAAACTAGGC CTAGTAAATG ACAAAGAGTC CAAAGATTCT 
ATGACAGAAG GGGAAAATCT TGAAGAGGAT GAAGAAGAAG AAGAAGGAGG AGCTGAGACA 
GAGGAACAGT CTGGAAATGA AAGTGAAGTG AACGAGCCAG AAGAAGAAGA GGGTTCTGAG 
GAGGAGGAGG AGGGAGAGGA GGAGGAAGAG GAGAATACAG ATTACCTCAC AGATTCCAAC 
AAGGAGAACG AGACTGATGA AGAAAATGCT GAGGTGATGA TTAAGGGAGG CGGACTGAAG 
CATGTTCCTT GTGTAGAAGA TGAGGACTTC ATTCAAGCTC TGGACAAAAT GATGCTAGAA 
AACCTTCAGC AGCGAAGTGG TGAGTCTGTT AAAGTGCACC AGCTTGATGT TGCTATCCCT 
TTGCATCTCA AAAGCCAGCT GAGGAAAGGG CCCCCGCTTG GCGGTGGGGA AGGAGAGACG 
GAGTCTGCAG ACACGATGCC ATTTGTCATG TTAACAAGAA AAGGCAATAA ACAGCAGTTT 
AAGATTCTCA ATGTGCCCAT GTCATCCCAA CTTGCTGCAA ATCATTGGAA CCAGCAACAA 
GCAGAACAAG AGGAGAGGAT GAGGATGAAA AAGCTCACCT TAGACATCAA TGAACGACAA 
GAGCAAGAAG ATTATCAAGA AATGTTGCAG TCTCTTGCAC AGCGCCCAGC TCCAGCAAAC 
ACTAATCGTG AGCGGCGGCC TCGTTACCAG CATCCAAAGG GAGCACCTAA TGCAGATCTA 
ATCTTTAAGA CTGGTGGGAG GAGACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Upf2 ( NM_001081132.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074601.1 Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), mRNA.

Price & Availability↑

CloneID	OMu01752
Clone ID Related Accession (Same CDS sequence)	XM_006497485.3 , NM_001081132.1 , XM_006497485.4
Accession Version	NM_001081132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-20
Organism	Mus musculus(house mouse)
Product	regulator of nonsense transcripts 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL928924.12, CR388026.3 and AL928735.9. On Feb 6, 2007 this sequence version replaced XM_140801.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.110498.1, SRR1660815.27036.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGCCAGCTG AGCGTAAAAA GTCAGCAAGT ATGGAAGAAA AAGAATCTTT ACTAAACAAC 
AAGGAAAAAG ACTGCAGTGA GAGGCGGCCA GTGAGCAGCA AGGAGAAACC AAGGGACGAC 
CTCAAGGTCA CTGCCAAGAA GGAGGTCAGT AAGGTCCCAG AAGACAAAAA GAAGAGACTG 
GAAGAAGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGAGGA AGAGAAGGTG 
AAGGCAGAGG AAGAGTTAAA GAAGAAGGAA GAGGAAGAAA AGAAGAAACA GGAAGAGGAA 
GAGAGGAAAA AGCAGGAGGA GCAGGCCAAG CGGCAGCAGG AAGAAGCGGC TGCTCAGCTG 
AAAGAGAAAG AAGAATCTCT TCAGCTTCAT CAGGAGGCCT GGGAACGCCA TCAATTACGA 
AAGGAACTTC GTAGCAAAAA CCAAAATGCT CCAGACAACC GACCAGAGGA AAATTTCTTT 
AGCCGCCTAG ATTCGAGCTT AAAGAAAAAT ACCGCTTTTG TCAAGAAACT AAAAACAATT 
ACAGAACAAC AGAGAGACTC CTTGTCTCAT GATTTTAATG GCCTGAATTT AAGCAAATAT 
ATTGCAGAAG CCGTAGCTTC CATTGTGGAG GCAAAATTAA AACTCTCTGA CGTGAACTGT 
GCTGCGCACC TCTGCTCTCT CTTTCACCAG CGGTACTCTG ACTTTGCGCC CTCTCTTCTT 
CAGGTGTGGA AAAAACATTT CGAAGCGAGG AAAGAGGAGA AAACGCCTAA CATTACCAAG 
CTAAGAACTG ACTTGCGTTT CATTGCAGAA TTGACAATAG TGGGAATTTT CACTGACAAG 
GAAGGTCTTT CCTTAATCTA CGAACAGCTA AAAAGTATTA TTAATGCTGA TCGAGAATCC 
CACACTCATG TTTCTGTGGT GATTAGTTTC TGTCGCCATT GTGGAGATGA CATTGCTGGA 
CTTGTACCGA GGAAAGTAAA AAGTGCTGCG GAGAAGTTTA ATTTGAGTTT TCCTCCTAGT 
GAGATCATTA GTCCGGAGAA GCAGCAGCCT TTCCAGAATC TTTTAAAAGA GTACTTTACG 
TCTTTGACCA AACACCTGAA AAGGGACCAC AGGGAGCTGC AGAACACTGA GAGGCAAAAC 
AGGCGTATTC TGCACTCTAA AGGCGAGCTA AGTGAGGACA GGCACAAGCA ATATGAGGAG 
TTTGCAATGT CTTACCAGAA GCTGCTGGCG AACTCTCAGT CCTTAGCGGA CCTTTTGGAT 
GAAAATATGC CCGATCTTCC TCAGGACAAA CCCACACCAG AAGAGCATGG GCCCGGAATC 
GACATATTTA CACCCGGCAA ACCTGGAGAG TATGACTTAG AAGGTGGCAT TTGGGAAGAT 
GAAGACGCTC GGAATTTTTA TGAAAACCTC ATCGATTTGA AAGCATTTGT TCCAGCCATC 
TTGTTCAAAG ACAATGAAAA AAGTCAGAAC AAAGATTCTA ACAAAGATGA TTCCAAAGAG 
GCAAAAGAAC CAAAGGATAA TAAGGAGGCA TCAAGTCCTG ATGATTTGGA ACTTGAGTTG 
GAGAATTTAG AAATTAATGA TGATACATTG GAGTTAGAAG GTGCAGATGA AGCTGAAGAT 
CTTACAAAGA AACTTCTTGA TGAACAAGAA CAAGAAGATG AAGAAGCCAG CACTGGATCT 
CATCTCAAAC TCATAGTAGA TGCTTTCCTC CAGCAGCTGC CCAACTGTGT CAACCGAGAC 
CTAATCGACA AGGCAGCCAT GGATTTTTGC ATGAACATGA ACACAAAGGC AAACAGGAAG 
AAGTTGGTGC GGGCACTCTT CATAGTTCCT AGGCAGAGGT TGGATTTGCT ACCATTTTAT 
GCAAGATTGG TTGCTACCTT GCACCCCTGC ATGTCTGATG TAGCAGAGGA TCTTTGTTCC 
ATGCTGAGGG GGGATTTCAG ATTTCATGTT CGAAAAAAGG ACCAGATCAA CATTGAAACA 
AAGAACAAAA CTGTCCGCTT TATTGGAGAG CTAACCAAGT TTAAGATGTT CACCAAAAAT 
GATACACTGC ATTGTCTAAA GATGCTTCTG TCAGATTTCT CTCATCATCA TATTGAAATG 
GCATGCACTC TACTGGAGAC CTGCGGAAGA TTTCTCTTCA GATCTCCAGA ATCACACCTG 
AGGACCAGTG TGCTTTTGGA GCAAATGATG AGAAAGAAAC AAGCAATGCA TCTTGATGCA 
CGATATGTTA CAATGGTAGA AAATGCATAT TATTACTGCA ACCCACCGCC CGCCGAAAAG 
ACCGTGAGAA AGAAACGCCC TCCTCTTCAG GAGTATGTCC GCAAGCTTCT GTACAAGGAT 
CTCTCTAAGG TCACGACGGA AAAGGTTTTG AGACAGATGC GAAAGTTGCC CTGGCAGGAC 
CAAGAAGTAA AAGACTATGT TATTTGTTGT ATGATAAACA TCTGGAACGT GAAATACAAC 
AGTATTCACT GTGTAGCCAA CCTCCTAGCA GGGCTAGTTC TCTACCAGGA GGATGTTGGG 
ATCCATGTTG TGGATGGCGT ACTCGAAGAT ATTCGATTGG GAATGGAGGT TAATCAACCT 
AAATTTAACC AGAGGCGTAT CAGTAGTGCC AAGTTCTTAG GAGAACTTTA TAATTACAGA 
ATGGTCGAAT CAGCTGTTAT TTTCAGAACT CTTTATTCTT TTACTTCATT TGGTGTCAAC 
CCTGATGGGT CTCCAAGTTC CCTGGACCCT CCTGAGCATC TCTTTAGAAT TAGACTAGTA 
TGCACTATTT TGGATACCTG TGGCCAGTAC TTTGACAGAG GTTCCAGTAA ACGAAAACTT 
GACTGCTTCC TTGTATATTT TCAGCGTTAT GTTTGGTGGA AGAAAAGTTT GGAGGTTTGG 
ACAAAAGACC ATCCATTTCC TATTGATATA GATTACATGA TCAGTGATAC ACTAGAACTG 
CTAAGACCAA AGATCAAACT CTGTAATTCT CTGGAAGAAT CCATCAGGCA GGTACAAGAC 
TTGGAACGAG AATTCTTAAT AAAACTAGGC CTAGTAAATG ACAAAGAGTC CAAAGATTCT 
ATGACAGAAG GGGAAAATCT TGAAGAGGAT GAAGAAGAAG AAGAAGGAGG AGCTGAGACA 
GAGGAACAGT CTGGAAATGA AAGTGAAGTG AACGAGCCAG AAGAAGAAGA GGGTTCTGAG 
GAGGAGGAGG AGGGAGAGGA GGAGGAAGAG GAGAATACAG ATTACCTCAC AGATTCCAAC 
AAGGAGAACG AGACTGATGA AGAAAATGCT GAGGTGATGA TTAAGGGAGG CGGACTGAAG 
CATGTTCCTT GTGTAGAAGA TGAGGACTTC ATTCAAGCTC TGGACAAAAT GATGCTAGAA 
AACCTTCAGC AGCGAAGTGG TGAGTCTGTT AAAGTGCACC AGCTTGATGT TGCTATCCCT 
TTGCATCTCA AAAGCCAGCT GAGGAAAGGG CCCCCGCTTG GCGGTGGGGA AGGAGAGACG 
GAGTCTGCAG ACACGATGCC ATTTGTCATG TTAACAAGAA AAGGCAATAA ACAGCAGTTT 
AAGATTCTCA ATGTGCCCAT GTCATCCCAA CTTGCTGCAA ATCATTGGAA CCAGCAACAA 
GCAGAACAAG AGGAGAGGAT GAGGATGAAA AAGCTCACCT TAGACATCAA TGAACGACAA 
GAGCAAGAAG ATTATCAAGA AATGTTGCAG TCTCTTGCAC AGCGCCCAGC TCCAGCAAAC 
ACTAATCGTG AGCGGCGGCC TCGTTACCAG CATCCAAAGG GAGCACCTAA TGCAGATCTA 
ATCTTTAAGA CTGGTGGGAG GAGACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074601.1
CDS	104..3913
Translation	MPAERKKSASMEEKESLLNNKEKDCSERRPVSSKEKPRDDLKVTAKKEVSKVPEDKKKRLEEDKRKKEDKERKKKEEEKVKAEEELKKKEEEEKKKQEEEERKKQEEQAKRQQEEAAAQLKEKEESLQLHQEAWERHQLRKELRSKNQNAPDNRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKLSDVNCAAHLCSLFHQRYSDFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDKEGLSLIYEQLKSIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSQNKDSNKDDSKEAKEPKDNKEASSPDDLELELENLEINDDTLELEGADEAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVRKKRPPLQEYVRKLLYKDLSKVTTEKVLRQMRKLPWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGSPSSLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHPFPIDIDYMISDTLELLRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKESKDSMTEGENLEEDEEEEEGGAETEEQSGNESEVNEPEEEEGSEEEEEGEEEEEENTDYLTDSNKENETDEENAEVMIKGGGLKHVPCVEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLKSQLRKGPPLGGGEGETESADTMPFVMLTRKGNKQQFKILNVPMSSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR

Target ORF information:

RefSeq Version	NM_001081132.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081132.1

ORF Insert Sequence:

ATGCCAGCTG AGCGTAAAAA GTCAGCAAGT ATGGAAGAAA AAGAATCTTT ACTAAACAAC 
AAGGAAAAAG ACTGCAGTGA GAGGCGGCCA GTGAGCAGCA AGGAGAAACC AAGGGACGAC 
CTCAAGGTCA CTGCCAAGAA GGAGGTCAGT AAGGTCCCAG AAGACAAAAA GAAGAGACTG 
GAAGAAGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGAGGA AGAGAAGGTG 
AAGGCAGAGG AAGAGTTAAA GAAGAAGGAA GAGGAAGAAA AGAAGAAACA GGAAGAGGAA 
GAGAGGAAAA AGCAGGAGGA GCAGGCCAAG CGGCAGCAGG AAGAAGCGGC TGCTCAGCTG 
AAAGAGAAAG AAGAATCTCT TCAGCTTCAT CAGGAGGCCT GGGAACGCCA TCAATTACGA 
AAGGAACTTC GTAGCAAAAA CCAAAATGCT CCAGACAACC GACCAGAGGA AAATTTCTTT 
AGCCGCCTAG ATTCGAGCTT AAAGAAAAAT ACCGCTTTTG TCAAGAAACT AAAAACAATT 
ACAGAACAAC AGAGAGACTC CTTGTCTCAT GATTTTAATG GCCTGAATTT AAGCAAATAT 
ATTGCAGAAG CCGTAGCTTC CATTGTGGAG GCAAAATTAA AACTCTCTGA CGTGAACTGT 
GCTGCGCACC TCTGCTCTCT CTTTCACCAG CGGTACTCTG ACTTTGCGCC CTCTCTTCTT 
CAGGTGTGGA AAAAACATTT CGAAGCGAGG AAAGAGGAGA AAACGCCTAA CATTACCAAG 
CTAAGAACTG ACTTGCGTTT CATTGCAGAA TTGACAATAG TGGGAATTTT CACTGACAAG 
GAAGGTCTTT CCTTAATCTA CGAACAGCTA AAAAGTATTA TTAATGCTGA TCGAGAATCC 
CACACTCATG TTTCTGTGGT GATTAGTTTC TGTCGCCATT GTGGAGATGA CATTGCTGGA 
CTTGTACCGA GGAAAGTAAA AAGTGCTGCG GAGAAGTTTA ATTTGAGTTT TCCTCCTAGT 
GAGATCATTA GTCCGGAGAA GCAGCAGCCT TTCCAGAATC TTTTAAAAGA GTACTTTACG 
TCTTTGACCA AACACCTGAA AAGGGACCAC AGGGAGCTGC AGAACACTGA GAGGCAAAAC 
AGGCGTATTC TGCACTCTAA AGGCGAGCTA AGTGAGGACA GGCACAAGCA ATATGAGGAG 
TTTGCAATGT CTTACCAGAA GCTGCTGGCG AACTCTCAGT CCTTAGCGGA CCTTTTGGAT 
GAAAATATGC CCGATCTTCC TCAGGACAAA CCCACACCAG AAGAGCATGG GCCCGGAATC 
GACATATTTA CACCCGGCAA ACCTGGAGAG TATGACTTAG AAGGTGGCAT TTGGGAAGAT 
GAAGACGCTC GGAATTTTTA TGAAAACCTC ATCGATTTGA AAGCATTTGT TCCAGCCATC 
TTGTTCAAAG ACAATGAAAA AAGTCAGAAC AAAGATTCTA ACAAAGATGA TTCCAAAGAG 
GCAAAAGAAC CAAAGGATAA TAAGGAGGCA TCAAGTCCTG ATGATTTGGA ACTTGAGTTG 
GAGAATTTAG AAATTAATGA TGATACATTG GAGTTAGAAG GTGCAGATGA AGCTGAAGAT 
CTTACAAAGA AACTTCTTGA TGAACAAGAA CAAGAAGATG AAGAAGCCAG CACTGGATCT 
CATCTCAAAC TCATAGTAGA TGCTTTCCTC CAGCAGCTGC CCAACTGTGT CAACCGAGAC 
CTAATCGACA AGGCAGCCAT GGATTTTTGC ATGAACATGA ACACAAAGGC AAACAGGAAG 
AAGTTGGTGC GGGCACTCTT CATAGTTCCT AGGCAGAGGT TGGATTTGCT ACCATTTTAT 
GCAAGATTGG TTGCTACCTT GCACCCCTGC ATGTCTGATG TAGCAGAGGA TCTTTGTTCC 
ATGCTGAGGG GGGATTTCAG ATTTCATGTT CGAAAAAAGG ACCAGATCAA CATTGAAACA 
AAGAACAAAA CTGTCCGCTT TATTGGAGAG CTAACCAAGT TTAAGATGTT CACCAAAAAT 
GATACACTGC ATTGTCTAAA GATGCTTCTG TCAGATTTCT CTCATCATCA TATTGAAATG 
GCATGCACTC TACTGGAGAC CTGCGGAAGA TTTCTCTTCA GATCTCCAGA ATCACACCTG 
AGGACCAGTG TGCTTTTGGA GCAAATGATG AGAAAGAAAC AAGCAATGCA TCTTGATGCA 
CGATATGTTA CAATGGTAGA AAATGCATAT TATTACTGCA ACCCACCGCC CGCCGAAAAG 
ACCGTGAGAA AGAAACGCCC TCCTCTTCAG GAGTATGTCC GCAAGCTTCT GTACAAGGAT 
CTCTCTAAGG TCACGACGGA AAAGGTTTTG AGACAGATGC GAAAGTTGCC CTGGCAGGAC 
CAAGAAGTAA AAGACTATGT TATTTGTTGT ATGATAAACA TCTGGAACGT GAAATACAAC 
AGTATTCACT GTGTAGCCAA CCTCCTAGCA GGGCTAGTTC TCTACCAGGA GGATGTTGGG 
ATCCATGTTG TGGATGGCGT ACTCGAAGAT ATTCGATTGG GAATGGAGGT TAATCAACCT 
AAATTTAACC AGAGGCGTAT CAGTAGTGCC AAGTTCTTAG GAGAACTTTA TAATTACAGA 
ATGGTCGAAT CAGCTGTTAT TTTCAGAACT CTTTATTCTT TTACTTCATT TGGTGTCAAC 
CCTGATGGGT CTCCAAGTTC CCTGGACCCT CCTGAGCATC TCTTTAGAAT TAGACTAGTA 
TGCACTATTT TGGATACCTG TGGCCAGTAC TTTGACAGAG GTTCCAGTAA ACGAAAACTT 
GACTGCTTCC TTGTATATTT TCAGCGTTAT GTTTGGTGGA AGAAAAGTTT GGAGGTTTGG 
ACAAAAGACC ATCCATTTCC TATTGATATA GATTACATGA TCAGTGATAC ACTAGAACTG 
CTAAGACCAA AGATCAAACT CTGTAATTCT CTGGAAGAAT CCATCAGGCA GGTACAAGAC 
TTGGAACGAG AATTCTTAAT AAAACTAGGC CTAGTAAATG ACAAAGAGTC CAAAGATTCT 
ATGACAGAAG GGGAAAATCT TGAAGAGGAT GAAGAAGAAG AAGAAGGAGG AGCTGAGACA 
GAGGAACAGT CTGGAAATGA AAGTGAAGTG AACGAGCCAG AAGAAGAAGA GGGTTCTGAG 
GAGGAGGAGG AGGGAGAGGA GGAGGAAGAG GAGAATACAG ATTACCTCAC AGATTCCAAC 
AAGGAGAACG AGACTGATGA AGAAAATGCT GAGGTGATGA TTAAGGGAGG CGGACTGAAG 
CATGTTCCTT GTGTAGAAGA TGAGGACTTC ATTCAAGCTC TGGACAAAAT GATGCTAGAA 
AACCTTCAGC AGCGAAGTGG TGAGTCTGTT AAAGTGCACC AGCTTGATGT TGCTATCCCT 
TTGCATCTCA AAAGCCAGCT GAGGAAAGGG CCCCCGCTTG GCGGTGGGGA AGGAGAGACG 
GAGTCTGCAG ACACGATGCC ATTTGTCATG TTAACAAGAA AAGGCAATAA ACAGCAGTTT 
AAGATTCTCA ATGTGCCCAT GTCATCCCAA CTTGCTGCAA ATCATTGGAA CCAGCAACAA 
GCAGAACAAG AGGAGAGGAT GAGGATGAAA AAGCTCACCT TAGACATCAA TGAACGACAA 
GAGCAAGAAG ATTATCAAGA AATGTTGCAG TCTCTTGCAC AGCGCCCAGC TCCAGCAAAC 
ACTAATCGTG AGCGGCGGCC TCGTTACCAG CATCCAAAGG GAGCACCTAA TGCAGATCTA 
ATCTTTAAGA CTGGTGGGAG GAGACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Upf2 ( XM_006497485.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497548.1 Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X1, mRNA.

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CloneID	OMu01752
Clone ID Related Accession (Same CDS sequence)	XM_006497485.3 , NM_001081132.1 , XM_006497485.4
Accession Version	XM_006497485.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	regulator of nonsense transcripts 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTG AGCGTAAAAA GTCAGCAAGT ATGGAAGAAA AAGAATCTTT ACTAAACAAC 
AAGGAAAAAG ACTGCAGTGA GAGGCGGCCA GTGAGCAGCA AGGAGAAACC AAGGGACGAC 
CTCAAGGTCA CTGCCAAGAA GGAGGTCAGT AAGGTCCCAG AAGACAAAAA GAAGAGACTG 
GAAGAAGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGAGGA AGAGAAGGTG 
AAGGCAGAGG AAGAGTTAAA GAAGAAGGAA GAGGAAGAAA AGAAGAAACA GGAAGAGGAA 
GAGAGGAAAA AGCAGGAGGA GCAGGCCAAG CGGCAGCAGG AAGAAGCGGC TGCTCAGCTG 
AAAGAGAAAG AAGAATCTCT TCAGCTTCAT CAGGAGGCCT GGGAACGCCA TCAATTACGA 
AAGGAACTTC GTAGCAAAAA CCAAAATGCT CCAGACAACC GACCAGAGGA AAATTTCTTT 
AGCCGCCTAG ATTCGAGCTT AAAGAAAAAT ACCGCTTTTG TCAAGAAACT AAAAACAATT 
ACAGAACAAC AGAGAGACTC CTTGTCTCAT GATTTTAATG GCCTGAATTT AAGCAAATAT 
ATTGCAGAAG CCGTAGCTTC CATTGTGGAG GCAAAATTAA AACTCTCTGA CGTGAACTGT 
GCTGCGCACC TCTGCTCTCT CTTTCACCAG CGGTACTCTG ACTTTGCGCC CTCTCTTCTT 
CAGGTGTGGA AAAAACATTT CGAAGCGAGG AAAGAGGAGA AAACGCCTAA CATTACCAAG 
CTAAGAACTG ACTTGCGTTT CATTGCAGAA TTGACAATAG TGGGAATTTT CACTGACAAG 
GAAGGTCTTT CCTTAATCTA CGAACAGCTA AAAAGTATTA TTAATGCTGA TCGAGAATCC 
CACACTCATG TTTCTGTGGT GATTAGTTTC TGTCGCCATT GTGGAGATGA CATTGCTGGA 
CTTGTACCGA GGAAAGTAAA AAGTGCTGCG GAGAAGTTTA ATTTGAGTTT TCCTCCTAGT 
GAGATCATTA GTCCGGAGAA GCAGCAGCCT TTCCAGAATC TTTTAAAAGA GTACTTTACG 
TCTTTGACCA AACACCTGAA AAGGGACCAC AGGGAGCTGC AGAACACTGA GAGGCAAAAC 
AGGCGTATTC TGCACTCTAA AGGCGAGCTA AGTGAGGACA GGCACAAGCA ATATGAGGAG 
TTTGCAATGT CTTACCAGAA GCTGCTGGCG AACTCTCAGT CCTTAGCGGA CCTTTTGGAT 
GAAAATATGC CCGATCTTCC TCAGGACAAA CCCACACCAG AAGAGCATGG GCCCGGAATC 
GACATATTTA CACCCGGCAA ACCTGGAGAG TATGACTTAG AAGGTGGCAT TTGGGAAGAT 
GAAGACGCTC GGAATTTTTA TGAAAACCTC ATCGATTTGA AAGCATTTGT TCCAGCCATC 
TTGTTCAAAG ACAATGAAAA AAGTCAGAAC AAAGATTCTA ACAAAGATGA TTCCAAAGAG 
GCAAAAGAAC CAAAGGATAA TAAGGAGGCA TCAAGTCCTG ATGATTTGGA ACTTGAGTTG 
GAGAATTTAG AAATTAATGA TGATACATTG GAGTTAGAAG GTGCAGATGA AGCTGAAGAT 
CTTACAAAGA AACTTCTTGA TGAACAAGAA CAAGAAGATG AAGAAGCCAG CACTGGATCT 
CATCTCAAAC TCATAGTAGA TGCTTTCCTC CAGCAGCTGC CCAACTGTGT CAACCGAGAC 
CTAATCGACA AGGCAGCCAT GGATTTTTGC ATGAACATGA ACACAAAGGC AAACAGGAAG 
AAGTTGGTGC GGGCACTCTT CATAGTTCCT AGGCAGAGGT TGGATTTGCT ACCATTTTAT 
GCAAGATTGG TTGCTACCTT GCACCCCTGC ATGTCTGATG TAGCAGAGGA TCTTTGTTCC 
ATGCTGAGGG GGGATTTCAG ATTTCATGTT CGAAAAAAGG ACCAGATCAA CATTGAAACA 
AAGAACAAAA CTGTCCGCTT TATTGGAGAG CTAACCAAGT TTAAGATGTT CACCAAAAAT 
GATACACTGC ATTGTCTAAA GATGCTTCTG TCAGATTTCT CTCATCATCA TATTGAAATG 
GCATGCACTC TACTGGAGAC CTGCGGAAGA TTTCTCTTCA GATCTCCAGA ATCACACCTG 
AGGACCAGTG TGCTTTTGGA GCAAATGATG AGAAAGAAAC AAGCAATGCA TCTTGATGCA 
CGATATGTTA CAATGGTAGA AAATGCATAT TATTACTGCA ACCCACCGCC CGCCGAAAAG 
ACCGTGAGAA AGAAACGCCC TCCTCTTCAG GAGTATGTCC GCAAGCTTCT GTACAAGGAT 
CTCTCTAAGG TCACGACGGA AAAGGTTTTG AGACAGATGC GAAAGTTGCC CTGGCAGGAC 
CAAGAAGTAA AAGACTATGT TATTTGTTGT ATGATAAACA TCTGGAACGT GAAATACAAC 
AGTATTCACT GTGTAGCCAA CCTCCTAGCA GGGCTAGTTC TCTACCAGGA GGATGTTGGG 
ATCCATGTTG TGGATGGCGT ACTCGAAGAT ATTCGATTGG GAATGGAGGT TAATCAACCT 
AAATTTAACC AGAGGCGTAT CAGTAGTGCC AAGTTCTTAG GAGAACTTTA TAATTACAGA 
ATGGTCGAAT CAGCTGTTAT TTTCAGAACT CTTTATTCTT TTACTTCATT TGGTGTCAAC 
CCTGATGGGT CTCCAAGTTC CCTGGACCCT CCTGAGCATC TCTTTAGAAT TAGACTAGTA 
TGCACTATTT TGGATACCTG TGGCCAGTAC TTTGACAGAG GTTCCAGTAA ACGAAAACTT 
GACTGCTTCC TTGTATATTT TCAGCGTTAT GTTTGGTGGA AGAAAAGTTT GGAGGTTTGG 
ACAAAAGACC ATCCATTTCC TATTGATATA GATTACATGA TCAGTGATAC ACTAGAACTG 
CTAAGACCAA AGATCAAACT CTGTAATTCT CTGGAAGAAT CCATCAGGCA GGTACAAGAC 
TTGGAACGAG AATTCTTAAT AAAACTAGGC CTAGTAAATG ACAAAGAGTC CAAAGATTCT 
ATGACAGAAG GGGAAAATCT TGAAGAGGAT GAAGAAGAAG AAGAAGGAGG AGCTGAGACA 
GAGGAACAGT CTGGAAATGA AAGTGAAGTG AACGAGCCAG AAGAAGAAGA GGGTTCTGAG 
GAGGAGGAGG AGGGAGAGGA GGAGGAAGAG GAGAATACAG ATTACCTCAC AGATTCCAAC 
AAGGAGAACG AGACTGATGA AGAAAATGCT GAGGTGATGA TTAAGGGAGG CGGACTGAAG 
CATGTTCCTT GTGTAGAAGA TGAGGACTTC ATTCAAGCTC TGGACAAAAT GATGCTAGAA 
AACCTTCAGC AGCGAAGTGG TGAGTCTGTT AAAGTGCACC AGCTTGATGT TGCTATCCCT 
TTGCATCTCA AAAGCCAGCT GAGGAAAGGG CCCCCGCTTG GCGGTGGGGA AGGAGAGACG 
GAGTCTGCAG ACACGATGCC ATTTGTCATG TTAACAAGAA AAGGCAATAA ACAGCAGTTT 
AAGATTCTCA ATGTGCCCAT GTCATCCCAA CTTGCTGCAA ATCATTGGAA CCAGCAACAA 
GCAGAACAAG AGGAGAGGAT GAGGATGAAA AAGCTCACCT TAGACATCAA TGAACGACAA 
GAGCAAGAAG ATTATCAAGA AATGTTGCAG TCTCTTGCAC AGCGCCCAGC TCCAGCAAAC 
ACTAATCGTG AGCGGCGGCC TCGTTACCAG CATCCAAAGG GAGCACCTAA TGCAGATCTA 
ATCTTTAAGA CTGGTGGGAG GAGACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497548.1
CDS	310..4119
Translation	MPAERKKSASMEEKESLLNNKEKDCSERRPVSSKEKPRDDLKVTAKKEVSKVPEDKKKRLEEDKRKKEDKERKKKEEEKVKAEEELKKKEEEEKKKQEEEERKKQEEQAKRQQEEAAAQLKEKEESLQLHQEAWERHQLRKELRSKNQNAPDNRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKLSDVNCAAHLCSLFHQRYSDFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDKEGLSLIYEQLKSIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSQNKDSNKDDSKEAKEPKDNKEASSPDDLELELENLEINDDTLELEGADEAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVRKKRPPLQEYVRKLLYKDLSKVTTEKVLRQMRKLPWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGSPSSLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHPFPIDIDYMISDTLELLRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKESKDSMTEGENLEEDEEEEEGGAETEEQSGNESEVNEPEEEEGSEEEEEGEEEEEENTDYLTDSNKENETDEENAEVMIKGGGLKHVPCVEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLKSQLRKGPPLGGGEGETESADTMPFVMLTRKGNKQQFKILNVPMSSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR

Target ORF information:

RefSeq Version	XM_006497485.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497485.3

ORF Insert Sequence:

ATGCCAGCTG AGCGTAAAAA GTCAGCAAGT ATGGAAGAAA AAGAATCTTT ACTAAACAAC 
AAGGAAAAAG ACTGCAGTGA GAGGCGGCCA GTGAGCAGCA AGGAGAAACC AAGGGACGAC 
CTCAAGGTCA CTGCCAAGAA GGAGGTCAGT AAGGTCCCAG AAGACAAAAA GAAGAGACTG 
GAAGAAGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGAGGA AGAGAAGGTG 
AAGGCAGAGG AAGAGTTAAA GAAGAAGGAA GAGGAAGAAA AGAAGAAACA GGAAGAGGAA 
GAGAGGAAAA AGCAGGAGGA GCAGGCCAAG CGGCAGCAGG AAGAAGCGGC TGCTCAGCTG 
AAAGAGAAAG AAGAATCTCT TCAGCTTCAT CAGGAGGCCT GGGAACGCCA TCAATTACGA 
AAGGAACTTC GTAGCAAAAA CCAAAATGCT CCAGACAACC GACCAGAGGA AAATTTCTTT 
AGCCGCCTAG ATTCGAGCTT AAAGAAAAAT ACCGCTTTTG TCAAGAAACT AAAAACAATT 
ACAGAACAAC AGAGAGACTC CTTGTCTCAT GATTTTAATG GCCTGAATTT AAGCAAATAT 
ATTGCAGAAG CCGTAGCTTC CATTGTGGAG GCAAAATTAA AACTCTCTGA CGTGAACTGT 
GCTGCGCACC TCTGCTCTCT CTTTCACCAG CGGTACTCTG ACTTTGCGCC CTCTCTTCTT 
CAGGTGTGGA AAAAACATTT CGAAGCGAGG AAAGAGGAGA AAACGCCTAA CATTACCAAG 
CTAAGAACTG ACTTGCGTTT CATTGCAGAA TTGACAATAG TGGGAATTTT CACTGACAAG 
GAAGGTCTTT CCTTAATCTA CGAACAGCTA AAAAGTATTA TTAATGCTGA TCGAGAATCC 
CACACTCATG TTTCTGTGGT GATTAGTTTC TGTCGCCATT GTGGAGATGA CATTGCTGGA 
CTTGTACCGA GGAAAGTAAA AAGTGCTGCG GAGAAGTTTA ATTTGAGTTT TCCTCCTAGT 
GAGATCATTA GTCCGGAGAA GCAGCAGCCT TTCCAGAATC TTTTAAAAGA GTACTTTACG 
TCTTTGACCA AACACCTGAA AAGGGACCAC AGGGAGCTGC AGAACACTGA GAGGCAAAAC 
AGGCGTATTC TGCACTCTAA AGGCGAGCTA AGTGAGGACA GGCACAAGCA ATATGAGGAG 
TTTGCAATGT CTTACCAGAA GCTGCTGGCG AACTCTCAGT CCTTAGCGGA CCTTTTGGAT 
GAAAATATGC CCGATCTTCC TCAGGACAAA CCCACACCAG AAGAGCATGG GCCCGGAATC 
GACATATTTA CACCCGGCAA ACCTGGAGAG TATGACTTAG AAGGTGGCAT TTGGGAAGAT 
GAAGACGCTC GGAATTTTTA TGAAAACCTC ATCGATTTGA AAGCATTTGT TCCAGCCATC 
TTGTTCAAAG ACAATGAAAA AAGTCAGAAC AAAGATTCTA ACAAAGATGA TTCCAAAGAG 
GCAAAAGAAC CAAAGGATAA TAAGGAGGCA TCAAGTCCTG ATGATTTGGA ACTTGAGTTG 
GAGAATTTAG AAATTAATGA TGATACATTG GAGTTAGAAG GTGCAGATGA AGCTGAAGAT 
CTTACAAAGA AACTTCTTGA TGAACAAGAA CAAGAAGATG AAGAAGCCAG CACTGGATCT 
CATCTCAAAC TCATAGTAGA TGCTTTCCTC CAGCAGCTGC CCAACTGTGT CAACCGAGAC 
CTAATCGACA AGGCAGCCAT GGATTTTTGC ATGAACATGA ACACAAAGGC AAACAGGAAG 
AAGTTGGTGC GGGCACTCTT CATAGTTCCT AGGCAGAGGT TGGATTTGCT ACCATTTTAT 
GCAAGATTGG TTGCTACCTT GCACCCCTGC ATGTCTGATG TAGCAGAGGA TCTTTGTTCC 
ATGCTGAGGG GGGATTTCAG ATTTCATGTT CGAAAAAAGG ACCAGATCAA CATTGAAACA 
AAGAACAAAA CTGTCCGCTT TATTGGAGAG CTAACCAAGT TTAAGATGTT CACCAAAAAT 
GATACACTGC ATTGTCTAAA GATGCTTCTG TCAGATTTCT CTCATCATCA TATTGAAATG 
GCATGCACTC TACTGGAGAC CTGCGGAAGA TTTCTCTTCA GATCTCCAGA ATCACACCTG 
AGGACCAGTG TGCTTTTGGA GCAAATGATG AGAAAGAAAC AAGCAATGCA TCTTGATGCA 
CGATATGTTA CAATGGTAGA AAATGCATAT TATTACTGCA ACCCACCGCC CGCCGAAAAG 
ACCGTGAGAA AGAAACGCCC TCCTCTTCAG GAGTATGTCC GCAAGCTTCT GTACAAGGAT 
CTCTCTAAGG TCACGACGGA AAAGGTTTTG AGACAGATGC GAAAGTTGCC CTGGCAGGAC 
CAAGAAGTAA AAGACTATGT TATTTGTTGT ATGATAAACA TCTGGAACGT GAAATACAAC 
AGTATTCACT GTGTAGCCAA CCTCCTAGCA GGGCTAGTTC TCTACCAGGA GGATGTTGGG 
ATCCATGTTG TGGATGGCGT ACTCGAAGAT ATTCGATTGG GAATGGAGGT TAATCAACCT 
AAATTTAACC AGAGGCGTAT CAGTAGTGCC AAGTTCTTAG GAGAACTTTA TAATTACAGA 
ATGGTCGAAT CAGCTGTTAT TTTCAGAACT CTTTATTCTT TTACTTCATT TGGTGTCAAC 
CCTGATGGGT CTCCAAGTTC CCTGGACCCT CCTGAGCATC TCTTTAGAAT TAGACTAGTA 
TGCACTATTT TGGATACCTG TGGCCAGTAC TTTGACAGAG GTTCCAGTAA ACGAAAACTT 
GACTGCTTCC TTGTATATTT TCAGCGTTAT GTTTGGTGGA AGAAAAGTTT GGAGGTTTGG 
ACAAAAGACC ATCCATTTCC TATTGATATA GATTACATGA TCAGTGATAC ACTAGAACTG 
CTAAGACCAA AGATCAAACT CTGTAATTCT CTGGAAGAAT CCATCAGGCA GGTACAAGAC 
TTGGAACGAG AATTCTTAAT AAAACTAGGC CTAGTAAATG ACAAAGAGTC CAAAGATTCT 
ATGACAGAAG GGGAAAATCT TGAAGAGGAT GAAGAAGAAG AAGAAGGAGG AGCTGAGACA 
GAGGAACAGT CTGGAAATGA AAGTGAAGTG AACGAGCCAG AAGAAGAAGA GGGTTCTGAG 
GAGGAGGAGG AGGGAGAGGA GGAGGAAGAG GAGAATACAG ATTACCTCAC AGATTCCAAC 
AAGGAGAACG AGACTGATGA AGAAAATGCT GAGGTGATGA TTAAGGGAGG CGGACTGAAG 
CATGTTCCTT GTGTAGAAGA TGAGGACTTC ATTCAAGCTC TGGACAAAAT GATGCTAGAA 
AACCTTCAGC AGCGAAGTGG TGAGTCTGTT AAAGTGCACC AGCTTGATGT TGCTATCCCT 
TTGCATCTCA AAAGCCAGCT GAGGAAAGGG CCCCCGCTTG GCGGTGGGGA AGGAGAGACG 
GAGTCTGCAG ACACGATGCC ATTTGTCATG TTAACAAGAA AAGGCAATAA ACAGCAGTTT 
AAGATTCTCA ATGTGCCCAT GTCATCCCAA CTTGCTGCAA ATCATTGGAA CCAGCAACAA 
GCAGAACAAG AGGAGAGGAT GAGGATGAAA AAGCTCACCT TAGACATCAA TGAACGACAA 
GAGCAAGAAG ATTATCAAGA AATGTTGCAG TCTCTTGCAC AGCGCCCAGC TCCAGCAAAC 
ACTAATCGTG AGCGGCGGCC TCGTTACCAG CATCCAAAGG GAGCACCTAA TGCAGATCTA 
ATCTTTAAGA CTGGTGGGAG GAGACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Upf2 ( XM_006497486.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497549.1 Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu29163
Clone ID Related Accession (Same CDS sequence)	XM_006497486.1
Accession Version	XM_006497486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	regulator of nonsense transcripts 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTG AGCGTAAAAA GTCAGCAAGT ATGGAAGAAA AAGAATCTTT ACTAAACAAC 
AAGGAAAAAG ACTGCAGTGA GAGGCGGCCA GTGAGCAGCA AGGAGAAACC AAGGGACGAC 
CTCAAGGTCA CTGCCAAGAA GGAGGTCAGT AAGGTCCCAG AAGACAAAAA GAAGAGACTG 
GAAGAAGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGAGGA AGAGAAGGTG 
AAGGCAGAGG AAGAGTTAAA GAAGAAGGAA GAGGAAGAAA AGAAGAAACA GGAAGAGGAA 
GAGAGGAAAA AGCAGGAGGA GCAGGCCAAG CGGCAGCAGG AAGAAGCGGC TGCTCAGCTG 
AAAGAGAAAG AAGAATCTCT TCAGCTTCAT CAGGAGGCCT GGGAACGCCA TCAATTACGA 
AAGGAACTTC GTAGCAAAAA CCAAAATGCT CCAGACAACC GACCAGAGGA AAATTTCTTT 
AGCCGCCTAG ATTCGAGCTT AAAGAAAAAT ACCGCTTTTG TCAAGAAACT AAAAACAATT 
ACAGAACAAC AGAGAGACTC CTTGTCTCAT GATTTTAATG GCCTGAATTT AAGCAAATAT 
ATTGCAGAAG CCGTAGCTTC CATTGTGGAG GCAAAATTAA AACTCTCTGA CGTGAACTGT 
GCTGCGCACC TCTGCTCTCT CTTTCACCAG CGGTACTCTG ACTTTGCGCC CTCTCTTCTT 
CAGGTGTGGA AAAAACATTT CGAAGCGAGG AAAGAGGAGA AAACGCCTAA CATTACCAAG 
CTAAGAACTG ACTTGCGTTT CATTGCAGAA TTGACAATAG TGGGAATTTT CACTGACAAG 
GAAGGTCTTT CCTTAATCTA CGAACAGCTA AAAAGTATTA TTAATGCTGA TCGAGAATCC 
CACACTCATG TTTCTGTGGT GATTAGTTTC TGTCGCCATT GTGGAGATGA CATTGCTGGA 
CTTGTACCGA GGAAAGTAAA AAGTGCTGCG GAGAAGTTTA ATTTGAGTTT TCCTCCTAGT 
GAGATCATTA GTCCGGAGAA GCAGCAGCCT TTCCAGAATC TTTTAAAAGA GTACTTTACG 
TCTTTGACCA AACACCTGAA AAGGGACCAC AGGGAGCTGC AGAACACTGA GAGGCAAAAC 
AGGCGTATTC TGCACTCTAA AGGCGAGCTA AGTGAGGACA GGCACAAGCA ATATGAGGAG 
TTTGCAATGT CTTACCAGAA GCTGCTGGCG AACTCTCAGT CCTTAGCGGA CCTTTTGGAT 
GAAAATATGC CCGATCTTCC TCAGGACAAA CCCACACCAG AAGAGCATGG GCCCGGAATC 
GACATATTTA CACCCGGCAA ACCTGGAGAG TATGACTTAG AAGGTGGCAT TTGGGAAGAT 
GAAGACGCTC GGAATTTTTA TGAAAACCTC ATCGATTTGA AAGCATTTGT TCCAGCCATC 
TTGTTCAAAG ACAATGAAAA AAGTCAGAAC AAAGATTCTA ACAAAGATGA TTCCAAAGAG 
GCAAAAGAAC CAAAGGATAA TAAGGAGGCA TCAAGTCCTG ATGATTTGGA ACTTGAGTTG 
GAGAATTTAG AAATTAATGA TGATACATTG GAGTTAGAAG GTGCAGATGA AGCTGAAGAT 
CTTACAAAGA AACTTCTTGA TGAACAAGAA CAAGAAGATG AAGAAGCCAG CACTGGATCT 
CATCTCAAAC TCATAGTAGA TGCTTTCCTC CAGCAGCTGC CCAACTGTGT CAACCGAGAC 
CTAATCGACA AGGCAGCCAT GGATTTTTGC ATGAACATGA ACACAAAGGC AAACAGGAAG 
AAGTTGGTGC GGGCACTCTT CATAGTTCCT AGGCAGAGGT TGGATTTGCT ACCATTTTAT 
GCAAGATTGG TTGCTACCTT GCACCCCTGC ATGTCTGATG TAGCAGAGGA TCTTTGTTCC 
ATGCTGAGGG GGGATTTCAG ATTTCATGTT CGAAAAAAGG ACCAGATCAA CATTGAAACA 
AAGAACAAAA CTGTCCGCTT TATTGGAGAG CTAACCAAGT TTAAGATGTT CACCAAAAAT 
GATACACTGC ATTGTCTAAA GATGCTTCTG TCAGATTTCT CTCATCATCA TATTGAAATG 
GCATGCACTC TACTGGAGAC CTGCGGAAGA TTTCTCTTCA GATCTCCAGA ATCACACCTG 
AGGACCAGTG TGCTTTTGGA GCAAATGATG AGAAAGAAAC AAGCAATGCA TCTTGATGCA 
CGATATGTTA CAATGGTAGA AAATGCATAT TATTACTGCA ACCCACCGCC CGCCGAAAAG 
ACCGTGAGAA AGAAACGCCC TCCTCTTCAG GAGTATGTCC GCAAGCTTCT GTACAAGGAT 
CTCTCTAAGG TCACGACGGA AAAGGTTTTG AGACAGATGC GAAAGTTGCC CTGGCAGGAC 
CAAGAAGTAA AAGACTATGT TATTTGTTGT ATGATAAACA TCTGGAACGT GAAATACAAC 
AGTATTCACT GTGTAGCCAA CCTCCTAGCA GGGCTAGTTC TCTACCAGGA GGATGTTGGG 
ATCCATGTTG TGGATGGCGT ACTCGAAGAT ATTCGATTGG GAATGGAGGT TAATCAACCT 
AAATTTAACC AGAGGCGTAT CAGTAGTGCC AAGTTCTTAG GAGAACTTTA TAATTACAGA 
ATGGTCGAAT CAGCTGTTAT TTTCAGAACT CTTTATTCTT TTACTTCATT TGGTGTCAAC 
CCTGATGGGT CTCCAAGTTC CCTGGACCCT CCTGAGCATC TCTTTAGAAT TAGACTAGTA 
TGCACTATTT TGGATACCTG TGGCCAGTAC TTTGACAGAG GTTCCAGTAA ACGAAAACTT 
GACTGCTTCC TTGTATATTT TCAGCGTTAT GTTTGGTGGA AGAAAAGTTT GGAGGTTTGG 
ACAAAAGACC ATCCATTTCC TATTGATATA GATTACATGA TCAGTGATAC ACTAGAACTG 
CTAAGACCAA AGATCAAACT CTGTAATTCT CTGGAAGAAT CCATCAGGCA GGTACAAGAC 
TTGGAACGAG AATTCTTAAT AAAACTAGGC CTAGTAAATG ACAAAGAGTC CAAAGATTCT 
ATGACAGAAG GGGAAAATCT TGAAGAGGAT GAAGAAGAAG AAGAAGGAGG AGCTGAGACA 
GAGGAACAGT CTGGAAATGA AAGTGAAGTG AACGAGCCAG AAGAAGAAGA GGGTTCTGAG 
GAGGAGGAGG AGGGAGAGGA GGAGGAAGAG GAGAATACAG ATTACCTCAC AGATTCCAAC 
AAGGAGAACG AGACTGATGA AGAAAATGCT GAGGTGATGA TTAAGGGAGG CGGACTGAAG 
CATGTTCCTT GTGTAGAAGA TGAGGACTTC ATTCAAGCTC TGGACAAAAT GATGCTAGAA 
AACCTTCAGC GAAGTGGTGA GTCTGTTAAA GTGCACCAGC TTGATGTTGC TATCCCTTTG 
CATCTCAAAA GCCAGCTGAG GAAAGGGCCC CCGCTTGGCG GTGGGGAAGG AGAGACGGAG 
TCTGCAGACA CGATGCCATT TGTCATGTTA ACAAGAAAAG GCAATAAACA GCAGTTTAAG 
ATTCTCAATG TGCCCATGTC ATCCCAACTT GCTGCAAATC ATTGGAACCA GCAACAAGCA 
GAACAAGAGG AGAGGATGAG GATGAAAAAG CTCACCTTAG ACATCAATGA ACGACAAGAG 
CAAGAAGATT ATCAAGAAAT GTTGCAGTCT CTTGCACAGC GCCCAGCTCC AGCAAACACT 
AATCGTGAGC GGCGGCCTCG TTACCAGCAT CCAAAGGGAG CACCTAATGC AGATCTAATC 
TTTAAGACTG GTGGGAGGAG ACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497549.1
CDS	126..3932
Translation	MPAERKKSASMEEKESLLNNKEKDCSERRPVSSKEKPRDDLKVTAKKEVSKVPEDKKKRLEEDKRKKEDKERKKKEEEKVKAEEELKKKEEEEKKKQEEEERKKQEEQAKRQQEEAAAQLKEKEESLQLHQEAWERHQLRKELRSKNQNAPDNRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKLSDVNCAAHLCSLFHQRYSDFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDKEGLSLIYEQLKSIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSQNKDSNKDDSKEAKEPKDNKEASSPDDLELELENLEINDDTLELEGADEAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVRKKRPPLQEYVRKLLYKDLSKVTTEKVLRQMRKLPWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGSPSSLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHPFPIDIDYMISDTLELLRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKESKDSMTEGENLEEDEEEEEGGAETEEQSGNESEVNEPEEEEGSEEEEEGEEEEEENTDYLTDSNKENETDEENAEVMIKGGGLKHVPCVEDEDFIQALDKMMLENLQRSGESVKVHQLDVAIPLHLKSQLRKGPPLGGGEGETESADTMPFVMLTRKGNKQQFKILNVPMSSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR

Target ORF information:

RefSeq Version	XM_006497486.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497486.1

ORF Insert Sequence:

ATGCCAGCTG AGCGTAAAAA GTCAGCAAGT ATGGAAGAAA AAGAATCTTT ACTAAACAAC 
AAGGAAAAAG ACTGCAGTGA GAGGCGGCCA GTGAGCAGCA AGGAGAAACC AAGGGACGAC 
CTCAAGGTCA CTGCCAAGAA GGAGGTCAGT AAGGTCCCAG AAGACAAAAA GAAGAGACTG 
GAAGAAGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGAGGA AGAGAAGGTG 
AAGGCAGAGG AAGAGTTAAA GAAGAAGGAA GAGGAAGAAA AGAAGAAACA GGAAGAGGAA 
GAGAGGAAAA AGCAGGAGGA GCAGGCCAAG CGGCAGCAGG AAGAAGCGGC TGCTCAGCTG 
AAAGAGAAAG AAGAATCTCT TCAGCTTCAT CAGGAGGCCT GGGAACGCCA TCAATTACGA 
AAGGAACTTC GTAGCAAAAA CCAAAATGCT CCAGACAACC GACCAGAGGA AAATTTCTTT 
AGCCGCCTAG ATTCGAGCTT AAAGAAAAAT ACCGCTTTTG TCAAGAAACT AAAAACAATT 
ACAGAACAAC AGAGAGACTC CTTGTCTCAT GATTTTAATG GCCTGAATTT AAGCAAATAT 
ATTGCAGAAG CCGTAGCTTC CATTGTGGAG GCAAAATTAA AACTCTCTGA CGTGAACTGT 
GCTGCGCACC TCTGCTCTCT CTTTCACCAG CGGTACTCTG ACTTTGCGCC CTCTCTTCTT 
CAGGTGTGGA AAAAACATTT CGAAGCGAGG AAAGAGGAGA AAACGCCTAA CATTACCAAG 
CTAAGAACTG ACTTGCGTTT CATTGCAGAA TTGACAATAG TGGGAATTTT CACTGACAAG 
GAAGGTCTTT CCTTAATCTA CGAACAGCTA AAAAGTATTA TTAATGCTGA TCGAGAATCC 
CACACTCATG TTTCTGTGGT GATTAGTTTC TGTCGCCATT GTGGAGATGA CATTGCTGGA 
CTTGTACCGA GGAAAGTAAA AAGTGCTGCG GAGAAGTTTA ATTTGAGTTT TCCTCCTAGT 
GAGATCATTA GTCCGGAGAA GCAGCAGCCT TTCCAGAATC TTTTAAAAGA GTACTTTACG 
TCTTTGACCA AACACCTGAA AAGGGACCAC AGGGAGCTGC AGAACACTGA GAGGCAAAAC 
AGGCGTATTC TGCACTCTAA AGGCGAGCTA AGTGAGGACA GGCACAAGCA ATATGAGGAG 
TTTGCAATGT CTTACCAGAA GCTGCTGGCG AACTCTCAGT CCTTAGCGGA CCTTTTGGAT 
GAAAATATGC CCGATCTTCC TCAGGACAAA CCCACACCAG AAGAGCATGG GCCCGGAATC 
GACATATTTA CACCCGGCAA ACCTGGAGAG TATGACTTAG AAGGTGGCAT TTGGGAAGAT 
GAAGACGCTC GGAATTTTTA TGAAAACCTC ATCGATTTGA AAGCATTTGT TCCAGCCATC 
TTGTTCAAAG ACAATGAAAA AAGTCAGAAC AAAGATTCTA ACAAAGATGA TTCCAAAGAG 
GCAAAAGAAC CAAAGGATAA TAAGGAGGCA TCAAGTCCTG ATGATTTGGA ACTTGAGTTG 
GAGAATTTAG AAATTAATGA TGATACATTG GAGTTAGAAG GTGCAGATGA AGCTGAAGAT 
CTTACAAAGA AACTTCTTGA TGAACAAGAA CAAGAAGATG AAGAAGCCAG CACTGGATCT 
CATCTCAAAC TCATAGTAGA TGCTTTCCTC CAGCAGCTGC CCAACTGTGT CAACCGAGAC 
CTAATCGACA AGGCAGCCAT GGATTTTTGC ATGAACATGA ACACAAAGGC AAACAGGAAG 
AAGTTGGTGC GGGCACTCTT CATAGTTCCT AGGCAGAGGT TGGATTTGCT ACCATTTTAT 
GCAAGATTGG TTGCTACCTT GCACCCCTGC ATGTCTGATG TAGCAGAGGA TCTTTGTTCC 
ATGCTGAGGG GGGATTTCAG ATTTCATGTT CGAAAAAAGG ACCAGATCAA CATTGAAACA 
AAGAACAAAA CTGTCCGCTT TATTGGAGAG CTAACCAAGT TTAAGATGTT CACCAAAAAT 
GATACACTGC ATTGTCTAAA GATGCTTCTG TCAGATTTCT CTCATCATCA TATTGAAATG 
GCATGCACTC TACTGGAGAC CTGCGGAAGA TTTCTCTTCA GATCTCCAGA ATCACACCTG 
AGGACCAGTG TGCTTTTGGA GCAAATGATG AGAAAGAAAC AAGCAATGCA TCTTGATGCA 
CGATATGTTA CAATGGTAGA AAATGCATAT TATTACTGCA ACCCACCGCC CGCCGAAAAG 
ACCGTGAGAA AGAAACGCCC TCCTCTTCAG GAGTATGTCC GCAAGCTTCT GTACAAGGAT 
CTCTCTAAGG TCACGACGGA AAAGGTTTTG AGACAGATGC GAAAGTTGCC CTGGCAGGAC 
CAAGAAGTAA AAGACTATGT TATTTGTTGT ATGATAAACA TCTGGAACGT GAAATACAAC 
AGTATTCACT GTGTAGCCAA CCTCCTAGCA GGGCTAGTTC TCTACCAGGA GGATGTTGGG 
ATCCATGTTG TGGATGGCGT ACTCGAAGAT ATTCGATTGG GAATGGAGGT TAATCAACCT 
AAATTTAACC AGAGGCGTAT CAGTAGTGCC AAGTTCTTAG GAGAACTTTA TAATTACAGA 
ATGGTCGAAT CAGCTGTTAT TTTCAGAACT CTTTATTCTT TTACTTCATT TGGTGTCAAC 
CCTGATGGGT CTCCAAGTTC CCTGGACCCT CCTGAGCATC TCTTTAGAAT TAGACTAGTA 
TGCACTATTT TGGATACCTG TGGCCAGTAC TTTGACAGAG GTTCCAGTAA ACGAAAACTT 
GACTGCTTCC TTGTATATTT TCAGCGTTAT GTTTGGTGGA AGAAAAGTTT GGAGGTTTGG 
ACAAAAGACC ATCCATTTCC TATTGATATA GATTACATGA TCAGTGATAC ACTAGAACTG 
CTAAGACCAA AGATCAAACT CTGTAATTCT CTGGAAGAAT CCATCAGGCA GGTACAAGAC 
TTGGAACGAG AATTCTTAAT AAAACTAGGC CTAGTAAATG ACAAAGAGTC CAAAGATTCT 
ATGACAGAAG GGGAAAATCT TGAAGAGGAT GAAGAAGAAG AAGAAGGAGG AGCTGAGACA 
GAGGAACAGT CTGGAAATGA AAGTGAAGTG AACGAGCCAG AAGAAGAAGA GGGTTCTGAG 
GAGGAGGAGG AGGGAGAGGA GGAGGAAGAG GAGAATACAG ATTACCTCAC AGATTCCAAC 
AAGGAGAACG AGACTGATGA AGAAAATGCT GAGGTGATGA TTAAGGGAGG CGGACTGAAG 
CATGTTCCTT GTGTAGAAGA TGAGGACTTC ATTCAAGCTC TGGACAAAAT GATGCTAGAA 
AACCTTCAGC GAAGTGGTGA GTCTGTTAAA GTGCACCAGC TTGATGTTGC TATCCCTTTG 
CATCTCAAAA GCCAGCTGAG GAAAGGGCCC CCGCTTGGCG GTGGGGAAGG AGAGACGGAG 
TCTGCAGACA CGATGCCATT TGTCATGTTA ACAAGAAAAG GCAATAAACA GCAGTTTAAG 
ATTCTCAATG TGCCCATGTC ATCCCAACTT GCTGCAAATC ATTGGAACCA GCAACAAGCA 
GAACAAGAGG AGAGGATGAG GATGAAAAAG CTCACCTTAG ACATCAATGA ACGACAAGAG 
CAAGAAGATT ATCAAGAAAT GTTGCAGTCT CTTGCACAGC GCCCAGCTCC AGCAAACACT 
AATCGTGAGC GGCGGCCTCG TTACCAGCAT CCAAAGGGAG CACCTAATGC AGATCTAATC 
TTTAAGACTG GTGGGAGGAG ACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Upf2 ( XM_006497487.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497550.1 Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu29164
Clone ID Related Accession (Same CDS sequence)	XM_006497487.1
Accession Version	XM_006497487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	regulator of nonsense transcripts 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTG AGCGTAAAAA GTCAGCAAGT ATGGAAGAAA AAGAATCTTT ACTAAACAAC 
AAGGAAAAAG ACTGCAGTGA GAGGCGGCCA GTGAGCAGCA AGGAGAAACC AAGGGACGAC 
CTCAAGGTCA CTGCCAAGAA GGAGGTCAGT AAGGTCCCAG AAGACAAAAA GAAGAGACTG 
GAAGAAGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGAGGA AGAGAAGGTG 
AAGGCAGAGG AAGAGTTAAA GAAGAAGGAA GAGGAAGAAA AGAAGAAACA GGAAGAGGAA 
GAGAGGAAAA AGCAGGAGGA GCAGGCCAAG CGGCAGCAGG AAGAAGCGGC TGCTCAGCTG 
AAAGAGAAAG AAGAATCTCT TCAGCTTCAT CAGGAGGCCT GGGAACGCCA TCAATTACGA 
AAGGAACTTC GTAGCAAAAA CCAAAATGCT CCAGACAACC GACCAGAGGA AAATTTCTTT 
AGCCGCCTAG ATTCGAGCTT AAAGAAAAAT ACCGCTTTTG TCAAGAAACT AAAAACAATT 
ACAGAACAAC AGAGAGACTC CTTGTCTCAT GATTTTAATG GCCTGAATTT AAGCAAATAT 
ATTGCAGAAG CCGTAGCTTC CATTGTGGAG GCAAAATTAA AACTCTCTGA CGTGAACTGT 
GCTGCGCACC TCTGCTCTCT CTTTCACCAG CGGTACTCTG ACTTTGCGCC CTCTCTTCTT 
CAGGTGTGGA AAAAACATTT CGAAGCGAGG AAAGAGGAGA AAACGCCTAA CATTACCAAG 
CTAAGAACTG ACTTGCGTTT CATTGCAGAA TTGACAATAG TGGGAATTTT CACTGACAAG 
GAAGGTCTTT CCTTAATCTA CGAACAGCTA AAAAGTATTA TTAATGCTGA TCGAGAATCC 
CACACTCATG TTTCTGTGGT GATTAGTTTC TGTCGCCATT GTGGAGATGA CATTGCTGGA 
CTTGTACCGA GGAAAGTAAA AAGTGCTGCG GAGAAGTTTA ATTTGAGTTT TCCTCCTAGT 
GAGATCATTA GTCCGGAGAA GCAGCAGCCT TTCCAGAATC TTTTAAAAGA GTACTTTACG 
TCTTTGACCA AACACCTGAA AAGGGACCAC AGGGAGCTGC AGAACACTGA GAGGCAAAAC 
AGGCGTATTC TGCACTCTAA AGGCGAGCTA AGTGAGGACA GGCACAAGCA ATATGAGGAG 
TTTGCAATGT CTTACCAGAA GCTGCTGGCG AACTCTCAGT CCTTAGCGGA CCTTTTGGAT 
GAAAATATGC CCGATCTTCC TCAGGACAAA CCCACACCAG AAGAGCATGG GCCCGGAATC 
GACATATTTA CACCCGGCAA ACCTGGAGAG TATGACTTAG AAGGTGGCAT TTGGGAAGAT 
GAAGACGCTC GGAATTTTTA TGAAAACCTC ATCGATTTGA AAGCATTTGT TCCAGCCATC 
TTGTTCAAAG ACAATGAAAA AAGTCAGAAC AAAGATTCTA ACAAAGATGA TTCCAAAGAG 
GCAAAAGAAC CAAAGGATAA TAAGGAGGCA TCAAGTCCTG ATGATTTGGA ACTTGAGTTG 
GAGAATTTAG AAATTAATGA TGATACATTG GAGTTAGAAG GTGCAGATGA AGCTGAAGAT 
CTTACAAAGA AACTTCTTGA TGAACAAGAA CAAGAAGATG AAGAAGCCAG CACTGGATCT 
CATCTCAAAC TCATAGTAGA TGCTTTCCTC CAGCAGCTGC CCAACTGTGT CAACCGAGAC 
CTAATCGACA AGGCAGCCAT GGATTTTTGC ATGAACATGA ACACAAAGGC AAACAGGAAG 
AAGTTGGTGC GGGCACTCTT CATAGTTCCT AGGCAGAGGT TGGATTTGCT ACCATTTTAT 
GCAAGATTGG TTGCTACCTT GCACCCCTGC ATGTCTGATG TAGCAGAGGA TCTTTGTTCC 
ATGCTGAGGG GGGATTTCAG ATTTCATGTT CGAAAAAAGG ACCAGATCAA CATTGAAACA 
AAGAACAAAA CTGTCCGCTT TATTGGAGAG CTAACCAAGT TTAAGATGTT CACCAAAAAT 
GATACACTGC ATTGTCTAAA GATGCTTCTG TCAGATTTCT CTCATCATCA TATTGAAATG 
GCATGCACTC TACTGGAGAC CTGCGGAAGA TTTCTCTTCA GATCTCCAGA ATCACACCTG 
AGGACCAGTG TGCTTTTGGA GCAAATGATG AGAAAGAAAC AAGCAATGCA TCTTGATGCA 
CGATATGTTA CAATGGTAGA AAATGCATAT TATTACTGCA ACCCACCGCC CGCCGAAAAG 
ACCGTGAGAA AGAAACGCCC TCCTCTTCAG GAGTATGTCC GCAAGCTTCT GTACAAGGAT 
CTCTCTAAGG TCACGACGGA AAAGGTTTTG AGACAGATGC GAAAGTTGCC CTGGCAGGAC 
CAAGAAGTAA AAGACTATGT TATTTGTTGT ATGATAAACA TCTGGAACGT GAAATACAAC 
AGTATTCACT GTGTAGCCAA CCTCCTAGCA GGGCTAGTTC TCTACCAGGA GGATGTTGGG 
ATCCATGTTG TGGATGGCGT ACTCGAAGAT ATTCGATTGG GAATGGAGGT TAATCAACCT 
AAATTTAACC AGAGGCGTAT CAGTAGTGCC AAGTTCTTAG GAGAACTTTA TAATTACAGA 
ATGGTCGAAT CAGCTGTTAT TTTCAGAACT CTTTATTCTT TTACTTCATT TGGTGTCAAC 
CCTGATGGGT CTCCAAGTTC CCTGGACCCT CCTGAGCATC TCTTTAGAAT TAGACTAGTA 
TGCACTATTT TGGATACCTG TGGCCAGTAC TTTGACAGAG GTTCCAGTAA ACGAAAACTT 
GACTGCTTCC TTGTATATTT TCAGCGTTAT GTTTGGTGGA AGAAAAGTTT GGAGGTTTGG 
ACAAAAGACC ATCCATTTCC TATTGATATA GATTACATGA TCAGTGATAC ACTAGAACTG 
CTAAGACCAA AGATCAAACT CTGTAATTCT CTGGAAGAAT CCATCAGGCA GGTACAAGAC 
TTGGAACGAG AATTCTTAAT AAAACTAGGC CTAGTAAATG ACAAAGAGTC CAAAGATTCT 
ATGACAGAAG GGGAAAATCT TGAAGAGGAT GAAGAAGAAG AAGAAGGAGG AGCTGAGACA 
GAGGAACAGT CTGGAAATGA AAGTGAAGTG AACGAGCCAG AAGAAGAAGA GGGTTCTGAG 
GAGGAGGAGG AGGGAGAGGA GGAGGAAGAG GAGAATACAG ATTACCTCAC AGATTCCAAC 
AAGGAGAACG AGACTGATGA AGAAAATGCT GAGGTGATGA TTAAGGGAGG CGGACTGAAG 
CATGTTCCTT GTGTAGAAGA TGAGGACTTC ATTCAAGCTC TGGACAAAAT GATGCTAGAA 
AACCTTCAGC AGCGAAGTGG TGAGTCTGTT AAAGTGCACC AGCTTGATGT TGCTATCCCT 
TTGCATCTCA AAAGCCAGCT GAGGAAAGGG CCCCCGCTTG GCGGTGGGGA AGGAGAGACG 
GAGTCTGCAG ACACGATGCC ATTTGTCATG TTAACAAGAA AAGGCAATAA ACAGCAGTTT 
AAGATTCTCA ATGTGCCCAT GTCATCCCAA CTTGCTGCAA ATCATTGGAA CCAGCAACAA 
GCAGAACAAG AGGAGAGGAT GAGGATGAAA AAGCTCACCT TAGACATCAA TGAACGACAA 
GAGCAAGAAG ATTATCAAGA AATGTTGCAG TCTCTTGCAC AGCGCCCAGC TCCAGCAAAC 
ACTAATCGTG AGCGGCGGCC TCGTTACCAG CATCCAAAGG GAGCACCTAA TGCAGATCTA 
ATCTTTAAGA CTGGTGGGAG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497550.1
CDS	126..3929
Translation	MPAERKKSASMEEKESLLNNKEKDCSERRPVSSKEKPRDDLKVTAKKEVSKVPEDKKKRLEEDKRKKEDKERKKKEEEKVKAEEELKKKEEEEKKKQEEEERKKQEEQAKRQQEEAAAQLKEKEESLQLHQEAWERHQLRKELRSKNQNAPDNRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKLSDVNCAAHLCSLFHQRYSDFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDKEGLSLIYEQLKSIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSQNKDSNKDDSKEAKEPKDNKEASSPDDLELELENLEINDDTLELEGADEAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVRKKRPPLQEYVRKLLYKDLSKVTTEKVLRQMRKLPWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGSPSSLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHPFPIDIDYMISDTLELLRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKESKDSMTEGENLEEDEEEEEGGAETEEQSGNESEVNEPEEEEGSEEEEEGEEEEEENTDYLTDSNKENETDEENAEVMIKGGGLKHVPCVEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLKSQLRKGPPLGGGEGETESADTMPFVMLTRKGNKQQFKILNVPMSSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGR

Target ORF information:

RefSeq Version	XM_006497487.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497487.1

ORF Insert Sequence:

ATGCCAGCTG AGCGTAAAAA GTCAGCAAGT ATGGAAGAAA AAGAATCTTT ACTAAACAAC 
AAGGAAAAAG ACTGCAGTGA GAGGCGGCCA GTGAGCAGCA AGGAGAAACC AAGGGACGAC 
CTCAAGGTCA CTGCCAAGAA GGAGGTCAGT AAGGTCCCAG AAGACAAAAA GAAGAGACTG 
GAAGAAGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGAGGA AGAGAAGGTG 
AAGGCAGAGG AAGAGTTAAA GAAGAAGGAA GAGGAAGAAA AGAAGAAACA GGAAGAGGAA 
GAGAGGAAAA AGCAGGAGGA GCAGGCCAAG CGGCAGCAGG AAGAAGCGGC TGCTCAGCTG 
AAAGAGAAAG AAGAATCTCT TCAGCTTCAT CAGGAGGCCT GGGAACGCCA TCAATTACGA 
AAGGAACTTC GTAGCAAAAA CCAAAATGCT CCAGACAACC GACCAGAGGA AAATTTCTTT 
AGCCGCCTAG ATTCGAGCTT AAAGAAAAAT ACCGCTTTTG TCAAGAAACT AAAAACAATT 
ACAGAACAAC AGAGAGACTC CTTGTCTCAT GATTTTAATG GCCTGAATTT AAGCAAATAT 
ATTGCAGAAG CCGTAGCTTC CATTGTGGAG GCAAAATTAA AACTCTCTGA CGTGAACTGT 
GCTGCGCACC TCTGCTCTCT CTTTCACCAG CGGTACTCTG ACTTTGCGCC CTCTCTTCTT 
CAGGTGTGGA AAAAACATTT CGAAGCGAGG AAAGAGGAGA AAACGCCTAA CATTACCAAG 
CTAAGAACTG ACTTGCGTTT CATTGCAGAA TTGACAATAG TGGGAATTTT CACTGACAAG 
GAAGGTCTTT CCTTAATCTA CGAACAGCTA AAAAGTATTA TTAATGCTGA TCGAGAATCC 
CACACTCATG TTTCTGTGGT GATTAGTTTC TGTCGCCATT GTGGAGATGA CATTGCTGGA 
CTTGTACCGA GGAAAGTAAA AAGTGCTGCG GAGAAGTTTA ATTTGAGTTT TCCTCCTAGT 
GAGATCATTA GTCCGGAGAA GCAGCAGCCT TTCCAGAATC TTTTAAAAGA GTACTTTACG 
TCTTTGACCA AACACCTGAA AAGGGACCAC AGGGAGCTGC AGAACACTGA GAGGCAAAAC 
AGGCGTATTC TGCACTCTAA AGGCGAGCTA AGTGAGGACA GGCACAAGCA ATATGAGGAG 
TTTGCAATGT CTTACCAGAA GCTGCTGGCG AACTCTCAGT CCTTAGCGGA CCTTTTGGAT 
GAAAATATGC CCGATCTTCC TCAGGACAAA CCCACACCAG AAGAGCATGG GCCCGGAATC 
GACATATTTA CACCCGGCAA ACCTGGAGAG TATGACTTAG AAGGTGGCAT TTGGGAAGAT 
GAAGACGCTC GGAATTTTTA TGAAAACCTC ATCGATTTGA AAGCATTTGT TCCAGCCATC 
TTGTTCAAAG ACAATGAAAA AAGTCAGAAC AAAGATTCTA ACAAAGATGA TTCCAAAGAG 
GCAAAAGAAC CAAAGGATAA TAAGGAGGCA TCAAGTCCTG ATGATTTGGA ACTTGAGTTG 
GAGAATTTAG AAATTAATGA TGATACATTG GAGTTAGAAG GTGCAGATGA AGCTGAAGAT 
CTTACAAAGA AACTTCTTGA TGAACAAGAA CAAGAAGATG AAGAAGCCAG CACTGGATCT 
CATCTCAAAC TCATAGTAGA TGCTTTCCTC CAGCAGCTGC CCAACTGTGT CAACCGAGAC 
CTAATCGACA AGGCAGCCAT GGATTTTTGC ATGAACATGA ACACAAAGGC AAACAGGAAG 
AAGTTGGTGC GGGCACTCTT CATAGTTCCT AGGCAGAGGT TGGATTTGCT ACCATTTTAT 
GCAAGATTGG TTGCTACCTT GCACCCCTGC ATGTCTGATG TAGCAGAGGA TCTTTGTTCC 
ATGCTGAGGG GGGATTTCAG ATTTCATGTT CGAAAAAAGG ACCAGATCAA CATTGAAACA 
AAGAACAAAA CTGTCCGCTT TATTGGAGAG CTAACCAAGT TTAAGATGTT CACCAAAAAT 
GATACACTGC ATTGTCTAAA GATGCTTCTG TCAGATTTCT CTCATCATCA TATTGAAATG 
GCATGCACTC TACTGGAGAC CTGCGGAAGA TTTCTCTTCA GATCTCCAGA ATCACACCTG 
AGGACCAGTG TGCTTTTGGA GCAAATGATG AGAAAGAAAC AAGCAATGCA TCTTGATGCA 
CGATATGTTA CAATGGTAGA AAATGCATAT TATTACTGCA ACCCACCGCC CGCCGAAAAG 
ACCGTGAGAA AGAAACGCCC TCCTCTTCAG GAGTATGTCC GCAAGCTTCT GTACAAGGAT 
CTCTCTAAGG TCACGACGGA AAAGGTTTTG AGACAGATGC GAAAGTTGCC CTGGCAGGAC 
CAAGAAGTAA AAGACTATGT TATTTGTTGT ATGATAAACA TCTGGAACGT GAAATACAAC 
AGTATTCACT GTGTAGCCAA CCTCCTAGCA GGGCTAGTTC TCTACCAGGA GGATGTTGGG 
ATCCATGTTG TGGATGGCGT ACTCGAAGAT ATTCGATTGG GAATGGAGGT TAATCAACCT 
AAATTTAACC AGAGGCGTAT CAGTAGTGCC AAGTTCTTAG GAGAACTTTA TAATTACAGA 
ATGGTCGAAT CAGCTGTTAT TTTCAGAACT CTTTATTCTT TTACTTCATT TGGTGTCAAC 
CCTGATGGGT CTCCAAGTTC CCTGGACCCT CCTGAGCATC TCTTTAGAAT TAGACTAGTA 
TGCACTATTT TGGATACCTG TGGCCAGTAC TTTGACAGAG GTTCCAGTAA ACGAAAACTT 
GACTGCTTCC TTGTATATTT TCAGCGTTAT GTTTGGTGGA AGAAAAGTTT GGAGGTTTGG 
ACAAAAGACC ATCCATTTCC TATTGATATA GATTACATGA TCAGTGATAC ACTAGAACTG 
CTAAGACCAA AGATCAAACT CTGTAATTCT CTGGAAGAAT CCATCAGGCA GGTACAAGAC 
TTGGAACGAG AATTCTTAAT AAAACTAGGC CTAGTAAATG ACAAAGAGTC CAAAGATTCT 
ATGACAGAAG GGGAAAATCT TGAAGAGGAT GAAGAAGAAG AAGAAGGAGG AGCTGAGACA 
GAGGAACAGT CTGGAAATGA AAGTGAAGTG AACGAGCCAG AAGAAGAAGA GGGTTCTGAG 
GAGGAGGAGG AGGGAGAGGA GGAGGAAGAG GAGAATACAG ATTACCTCAC AGATTCCAAC 
AAGGAGAACG AGACTGATGA AGAAAATGCT GAGGTGATGA TTAAGGGAGG CGGACTGAAG 
CATGTTCCTT GTGTAGAAGA TGAGGACTTC ATTCAAGCTC TGGACAAAAT GATGCTAGAA 
AACCTTCAGC AGCGAAGTGG TGAGTCTGTT AAAGTGCACC AGCTTGATGT TGCTATCCCT 
TTGCATCTCA AAAGCCAGCT GAGGAAAGGG CCCCCGCTTG GCGGTGGGGA AGGAGAGACG 
GAGTCTGCAG ACACGATGCC ATTTGTCATG TTAACAAGAA AAGGCAATAA ACAGCAGTTT 
AAGATTCTCA ATGTGCCCAT GTCATCCCAA CTTGCTGCAA ATCATTGGAA CCAGCAACAA 
GCAGAACAAG AGGAGAGGAT GAGGATGAAA AAGCTCACCT TAGACATCAA TGAACGACAA 
GAGCAAGAAG ATTATCAAGA AATGTTGCAG TCTCTTGCAC AGCGCCCAGC TCCAGCAAAC 
ACTAATCGTG AGCGGCGGCC TCGTTACCAG CATCCAAAGG GAGCACCTAA TGCAGATCTA 
ATCTTTAAGA CTGGTGGGAG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nonsense in the testis: multiple roles for nonsense-mediated decay revealed in male reproduction.
Biology of reproduction96(5)939-947(2017 May)
MacDonald CC,Grozdanov PN

UPF2-Dependent Nonsense-Mediated mRNA Decay Pathway Is Essential for Spermatogenesis by Selectively Eliminating Longer 3'UTR Transcripts.
PLoS genetics12(5)e1005863(2016 May)
Bao J,Vitting-Seerup K,Waage J,Tang C,Ge Y,Porse BT,Yan W

Chromatoid Body Protein TDRD6 Supports Long 3' UTR Triggered Nonsense Mediated mRNA Decay.
PLoS genetics12(5)e1005857(2016 May)
Fanourgakis G,Lesche M,Akpinar M,Dahl A,Jessberger R

Smg6/Est1 licenses embryonic stem cell differentiation via nonsense-mediated mRNA decay.
The EMBO journal34(12)1630-47(2015 Jun)
Li T,Shi Y,Wang P,Guachalla LM,Sun B,Joerss T,Chen YS,Groth M,Krueger A,Platzer M,Yang YG,Rudolph KL,Wang ZQ

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Upf2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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