gRNAs
The following Pkn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pkn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu02273 | |
| Clone ID Related Accession (Same CDS sequence) | XM_030243621.1 , NM_001199593.1 | |
| Accession Version | NM_001199593.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-27 | |
| Organism | Mus musculus(house mouse) | |
| Product | serine/threonine-protein kinase N1 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC164432.3, AK089431.1, AK149649.1 and CX568360.1. Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK149649.1, AK150062.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCAGCTG ATCCCCCTTT GGATTCGGAG CTGGAGAGTG AGCCTCGAAG CTGGTCGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001186522.1 |
| CDS | 67..2922 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001199593.1 |
1 | ATGGCAGCTG ATCCCCCTTT GGATTCGGAG CTGGAGAGTG AGCCTCGAAG CTGGTCGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu19239 | |
| Clone ID Related Accession (Same CDS sequence) | NM_177262.4 | |
| Accession Version | NM_177262.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-26 | |
| Organism | Mus musculus(house mouse) | |
| Product | serine/threonine-protein kinase N1 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC164432.3, BC052923.1 and CX568360.1. On Dec 9, 2010 this sequence version replaced NM_177262.3. Transcript Variant: This variant (2) contains a different segment for its 5' UTR and 5' coding region, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct N-terminus when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170556.1, BC052923.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGCCGGCG ACGCCGTACA GAGTGAGCCT CGAAGCTGGT CGCTGCTGGA ACAGCTGGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_796236.2 |
| CDS | 193..3033 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_177262.4 |
1 | ATGGCCGGCG ACGCCGTACA GAGTGAGCCT CGAAGCTGGT CGCTGCTGGA ACAGCTGGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu02273 | |
| Clone ID Related Accession (Same CDS sequence) | XM_030243621.1 , NM_001199593.1 | |
| Accession Version | XM_030243621.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | serine/threonine-protein kinase N1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCAGCTG ATCCCCCTTT GGATTCGGAG CTGGAGAGTG AGCCTCGAAG CTGGTCGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_030099481.1 |
| CDS | 337..3192 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_030243621.1 |
1 | ATGGCAGCTG ATCCCCCTTT GGATTCGGAG CTGGAGAGTG AGCCTCGAAG CTGGTCGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Loss of Protein Kinase Novel 1 (PKN1) is associated with mild systolic and diastolic contractile dysfunction, increased phospholamban Thr17 phosphorylation, and exacerbated ischaemia-reperfusion injury. |
The protein kinase C super-family member PKN is regulated by mTOR and influences differentiation during prostate cancer progression. |
Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1. |
