Pgap3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pgap3
Entrez Gene ID	320655
Full Name	post-GPI attachment to proteins 3
Synonyms	CAB2,D430035D22Rik,Perld1
General protein information	Preferred Names post-GPI attachment to proteins 3 Names post-GPI attachment to proteins factor 3 PER1-like domain-containing protein 1 per1-like domain containing 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 D

mRNA and Protein(s)

mRNA	Protein	Name
XM_006533550.4	XP_006533613.1	post-GPI attachment to proteins factor 3 isoform X2
XM_006533549.3	XP_006533612.1	post-GPI attachment to proteins factor 3 isoform X1
XM_006533550.3	XP_006533613.1	post-GPI attachment to proteins factor 3 isoform X2
XM_006533549.4	XP_006533612.1	post-GPI attachment to proteins factor 3 isoform X1
NM_001033537.2	NP_001028709.2	post-GPI attachment to proteins factor 3 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PGAP3	XP_548142.4
Kluyveromyces lactis	KLLA0C02101g	XP_452290.1
Magnaporthe oryzae (rice blast fungus)	MGG_04527	XP_003713578.1
Arabidopsis thaliana (thale cress)	AT1G16560	NP_973843.1
Homo sapiens (human)	PGAP3	NP_219487.3
Drosophila melanogaster (fruit fly)	CG3271	NP_610223.1
Neurospora crassa	NCU08609	XP_964817.2
Oryza sativa (rice)	Os05g0220100	NP_001054945.1
Pan troglodytes (chimpanzee)	PGAP3	XP_511460.1
Mus musculus (house mouse)	Pgap3	NP_001028709.2
Rattus norvegicus (Norway rat)	Perld1	NP_001137367.1
Saccharomyces cerevisiae (baker's yeast)	PER1	NP_009973.1
Eremothecium gossypii	AGOS_AFR678C	NP_986226.2
Schizosaccharomyces pombe (fission yeast)	SPAC823.07	NP_593834.1
Bos taurus (cattle)	PGAP3	NP_001098833.1
Gallus gallus (chicken)	PGAP3	XP_003642860.1
Xenopus tropicalis (tropical clawed frog)	pgap3	NP_001072247.1
	PGAP3	XP_001090781.1
Danio rerio (zebrafish)	zgc:171485	NP_001108063.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005392	XP_315401.4

Gene Ontology
Bibliography

Related articles in PubMed

Significance of glycosylphosphatidylinositol-anchored protein enrichment in lipid rafts for the control of autoimmunity.
Wang Y, Murakami Y, Yasui T, Wakana S, Kikutani H, Kinoshita T, Maeda Y
The Journal of biological chemistry288(35)25490-9(2013 Aug)

Enhanced response of T lymphocytes from Pgap3 knockout mouse: Insight into roles of fatty acid remodeling of GPI anchored proteins.
Murakami H, Wang Y, Hasuwa H, Maeda Y, Kinoshita T, Murakami Y
Biochemical and biophysical research communications417(4)1235-41(2012 Jan)

A mouse knockout library for secreted and transmembrane proteins.
Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M, Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ
Nature biotechnology28(7)749-55(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the response of T cells was enhanced in Pgap3 knockout mice in both in vitro and in vivo studies, including alloreactive response, antigen-specific immune response, and experimental autoimmune encephalomyelitis.
Title: Enhanced response of T lymphocytes from Pgap3 knockout mouse: Insight into roles of fatty acid remodeling of GPI anchored proteins.

The following Pgap3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pgap3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12719	NM_001033537.2 Latest version!	Mus musculus post-GPI attachment to proteins 3 (Pgap3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu46472	XM_006533549.4 Latest version!	Mus musculus post-GPI attachment to proteins 3 (Pgap3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu46473	XM_006533550.4 Latest version!	Mus musculus post-GPI attachment to proteins 3 (Pgap3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu46473	XM_006533550.3	Mus musculus post-GPI attachment to proteins 3 (Pgap3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu46472	XM_006533549.3	Mus musculus post-GPI attachment to proteins 3 (Pgap3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pgap3 ( NM_001033537.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028709.2 Mus musculus post-GPI attachment to proteins 3 (Pgap3), mRNA.

Price & Availability↑

CloneID	OMu12719
Clone ID Related Accession (Same CDS sequence)	NM_001033537.2
Accession Version	NM_001033537.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	post-GPI attachment to proteins factor 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591390.8. On Sep 14, 2006 this sequence version replaced NM_001033537.1. ##Evidence-Data-START## Transcript exon combination :: AK078974.1, AK170817.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGC GGACGGCGCC GCTGCTGCTG CTAACCTTGG CAGTGGGACT CGCGGGGGGT 
TCCCAGGGCG ACCGCGAGCC CGTGTACCGC GACTGCGTGC TCCGATGCGA GGAACGGAAC 
TGTTCGGGAG ACGCACTGAA GCACTTCCGC TCCCGCCAGC CAATCTACAT GAGCCTAGCT 
GGCTGGACCT GTCGGGACGA CTGCAAGTAT GAGTGTATGT GGTTCACTGT TGGCCTCTAC 
CTTCAGGAGG GCCACAGAGT GCCTCAGTTC CATGGCAAGT GGCCCTTCTC CCGGTTCCTG 
TTCATCCAAG AGCCAGCTTC TGCCGTGGCC TCACTCCTCA ATGGCCTGGC CAGCCTGGTG 
ATGCTCTGCC GCTATCGCGC CTCTGTGCCA GCCTCCTCCC CCATGTACCA TACCTGCATG 
GCCTTCGCTT GGGTGTCTCT CAATGCTTGG TTCTGGTCCA CAGTCTTCCA TACCAGGGAC 
ACTGACCTCA CAGAGAAGAT GGACTACTTC TGCGCTTCAG CTGTCATCCT GCATTCTGTT 
TACCTGTGCT GTGTCAGGAC CGTGGGACTA CAGCACCCGT CTGTGGCCAG TGCCTTCGGT 
GCCCTGCTGC TGCTGCTGCT GACAGGACAC ATATCCTACC TGAGCCTTGT CCATTTCGAC 
TACGGCTACA ACATGATGGC CAACGTGGCT ATCGGCCTGG TGAACCTGGC GTGGTGGCTG 
GTCTGGTGCC TACGGAACCG CCAGCGCCTC CCTCACACCC GCAGGTGTAT GGTGGTGGTA 
GTGCTGCTGC AGGGGCTGTC CCTGCTGGAA TTGCTTGACT TCCCGCCCCT CTTCTGGGTC 
CTGGACGCCC ACGCCATCTG GCACATCAGC ACCATCCCGG TCCACACCCT CTTTTTCAGG 
TTTCTGGAAG ATGACAGCTT GTACCTACTG AAGGAATCTG GAGCCATGTT CAAGCTGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028709.2
CDS	96..1058
Translation	MAKRTAPLLLLTLAVGLAGGSQGDREPVYRDCVLRCEERNCSGDALKHFRSRQPIYMSLAGWTCRDDCKYECMWFTVGLYLQEGHRVPQFHGKWPFSRFLFIQEPASAVASLLNGLASLVMLCRYRASVPASSPMYHTCMAFAWVSLNAWFWSTVFHTRDTDLTEKMDYFCASAVILHSVYLCCVRTVGLQHPSVASAFGALLLLLLTGHISYLSLVHFDYGYNMMANVAIGLVNLAWWLVWCLRNRQRLPHTRRCMVVVVLLQGLSLLELLDFPPLFWVLDAHAIWHISTIPVHTLFFRFLEDDSLYLLKESGAMFKLD

Target ORF information:

RefSeq Version	NM_001033537.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus post-GPI attachment to proteins 3 (Pgap3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033537.2

ORF Insert Sequence:

ATGGCCAAGC GGACGGCGCC GCTGCTGCTG CTAACCTTGG CAGTGGGACT CGCGGGGGGT 
TCCCAGGGCG ACCGCGAGCC CGTGTACCGC GACTGCGTGC TCCGATGCGA GGAACGGAAC 
TGTTCGGGAG ACGCACTGAA GCACTTCCGC TCCCGCCAGC CAATCTACAT GAGCCTAGCT 
GGCTGGACCT GTCGGGACGA CTGCAAGTAT GAGTGTATGT GGTTCACTGT TGGCCTCTAC 
CTTCAGGAGG GCCACAGAGT GCCTCAGTTC CATGGCAAGT GGCCCTTCTC CCGGTTCCTG 
TTCATCCAAG AGCCAGCTTC TGCCGTGGCC TCACTCCTCA ATGGCCTGGC CAGCCTGGTG 
ATGCTCTGCC GCTATCGCGC CTCTGTGCCA GCCTCCTCCC CCATGTACCA TACCTGCATG 
GCCTTCGCTT GGGTGTCTCT CAATGCTTGG TTCTGGTCCA CAGTCTTCCA TACCAGGGAC 
ACTGACCTCA CAGAGAAGAT GGACTACTTC TGCGCTTCAG CTGTCATCCT GCATTCTGTT 
TACCTGTGCT GTGTCAGGAC CGTGGGACTA CAGCACCCGT CTGTGGCCAG TGCCTTCGGT 
GCCCTGCTGC TGCTGCTGCT GACAGGACAC ATATCCTACC TGAGCCTTGT CCATTTCGAC 
TACGGCTACA ACATGATGGC CAACGTGGCT ATCGGCCTGG TGAACCTGGC GTGGTGGCTG 
GTCTGGTGCC TACGGAACCG CCAGCGCCTC CCTCACACCC GCAGGTGTAT GGTGGTGGTA 
GTGCTGCTGC AGGGGCTGTC CCTGCTGGAA TTGCTTGACT TCCCGCCCCT CTTCTGGGTC 
CTGGACGCCC ACGCCATCTG GCACATCAGC ACCATCCCGG TCCACACCCT CTTTTTCAGG 
TTTCTGGAAG ATGACAGCTT GTACCTACTG AAGGAATCTG GAGCCATGTT CAAGCTGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pgap3 ( XM_006533549.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533612.1 Mus musculus post-GPI attachment to proteins 3 (Pgap3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46472
Clone ID Related Accession (Same CDS sequence)	XM_006533549.3 , XM_006533549.4
Accession Version	XM_006533549.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	post-GPI attachment to proteins factor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533549.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC GGACGGCGCC GCTGCTGCTG CTAACCTTGG CAGTGGGACT CGCGGGGGGT 
TCCCAGGGCG ACCGCGAGCC CGTGTACCGC GACTGCGTGC TCCGATGCGA GGAACGGAAC 
TGTTCGGGAG ACGCACTGAA GCACTTCCGC TCCCGCCAGC CAATCTACAT GAGCCTAGCT 
GGCTGGACCT GTCGGGACGA CTGCAAGTAT GAGTGTATGT GGTTCACTGT TGGCCTCTAC 
CTTCAGGAGG GCCACAGAGT GCCTCAGTTC CATGGCAAGG TGTCTCTCAA TGCTTGGTTC 
TGGTCCACAG TCTTCCATAC CAGGGACACT GACCTCACAG AGAAGATGGA CTACTTCTGC 
GCTTCAGCTG TCATCCTGCA TTCTGTTTAC CTGTGCTGTG TCAGGACCGT GGGACTACAG 
CACCCGTCTG TGGCCAGTGC CTTCGGTGCC CTGCTGCTGC TGCTGCTGAC AGGACACATA 
TCCTACCTGA GCCTTGTCCA TTTCGACTAC GGCTACAACA TGATGGCCAA CGTGGCTATC 
GGCCTGGTGA ACCTGGCGTG GTGGCTGGTC TGGTGCCTAC GGAACCGCCA GCGCCTCCCT 
CACACCCGCA GGTGTATGGT GGTGGTAGTG CTGCTGCAGG GGCTGTCCCT GCTGGAATTG 
CTTGACTTCC CGCCCCTCTT CTGGGTCCTG GACGCCCACG CCATCTGGCA CATCAGCACC 
ATCCCGGTCC ACACCCTCTT TTTCAGGTTT CTGGAAGATG ACAGCTTGTA CCTACTGAAG 
GAATCTGGAG CCATGTTCAA GCTGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533612.1
CDS	90..899
Translation	MAKRTAPLLLLTLAVGLAGGSQGDREPVYRDCVLRCEERNCSGDALKHFRSRQPIYMSLAGWTCRDDCKYECMWFTVGLYLQEGHRVPQFHGKVSLNAWFWSTVFHTRDTDLTEKMDYFCASAVILHSVYLCCVRTVGLQHPSVASAFGALLLLLLTGHISYLSLVHFDYGYNMMANVAIGLVNLAWWLVWCLRNRQRLPHTRRCMVVVVLLQGLSLLELLDFPPLFWVLDAHAIWHISTIPVHTLFFRFLEDDSLYLLKESGAMFKLD

Target ORF information:

RefSeq Version	XM_006533549.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus post-GPI attachment to proteins 3 (Pgap3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533549.3

ORF Insert Sequence:

ATGGCCAAGC GGACGGCGCC GCTGCTGCTG CTAACCTTGG CAGTGGGACT CGCGGGGGGT 
TCCCAGGGCG ACCGCGAGCC CGTGTACCGC GACTGCGTGC TCCGATGCGA GGAACGGAAC 
TGTTCGGGAG ACGCACTGAA GCACTTCCGC TCCCGCCAGC CAATCTACAT GAGCCTAGCT 
GGCTGGACCT GTCGGGACGA CTGCAAGTAT GAGTGTATGT GGTTCACTGT TGGCCTCTAC 
CTTCAGGAGG GCCACAGAGT GCCTCAGTTC CATGGCAAGG TGTCTCTCAA TGCTTGGTTC 
TGGTCCACAG TCTTCCATAC CAGGGACACT GACCTCACAG AGAAGATGGA CTACTTCTGC 
GCTTCAGCTG TCATCCTGCA TTCTGTTTAC CTGTGCTGTG TCAGGACCGT GGGACTACAG 
CACCCGTCTG TGGCCAGTGC CTTCGGTGCC CTGCTGCTGC TGCTGCTGAC AGGACACATA 
TCCTACCTGA GCCTTGTCCA TTTCGACTAC GGCTACAACA TGATGGCCAA CGTGGCTATC 
GGCCTGGTGA ACCTGGCGTG GTGGCTGGTC TGGTGCCTAC GGAACCGCCA GCGCCTCCCT 
CACACCCGCA GGTGTATGGT GGTGGTAGTG CTGCTGCAGG GGCTGTCCCT GCTGGAATTG 
CTTGACTTCC CGCCCCTCTT CTGGGTCCTG GACGCCCACG CCATCTGGCA CATCAGCACC 
ATCCCGGTCC ACACCCTCTT TTTCAGGTTT CTGGAAGATG ACAGCTTGTA CCTACTGAAG 
GAATCTGGAG CCATGTTCAA GCTGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pgap3 ( XM_006533550.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533613.1 Mus musculus post-GPI attachment to proteins 3 (Pgap3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu46473
Clone ID Related Accession (Same CDS sequence)	XM_006533550.4 , XM_006533550.3
Accession Version	XM_006533550.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	post-GPI attachment to proteins factor 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533550.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTTCA CTGTTGGCCT CTACCTTCAG GAGGGCCACA GAGTGCCTCA GTTCCATGGC 
AAGTGGCCCT TCTCCCGGTT CCTGTTCATC CAAGAGCCAG CTTCTGCCGT GGCCTCACTC 
CTCAATGGCC TGGCCAGCCT GGTGATGCTC TGCCGCTATC GCGCCTCTGT GCCAGCCTCC 
TCCCCCATGT ACCATACCTG CATGGCCTTC GCTTGGGTGT CTCTCAATGC TTGGTTCTGG 
TCCACAGTCT TCCATACCAG GGACACTGAC CTCACAGAGA AGATGGACTA CTTCTGCGCT 
TCAGCTGTCA TCCTGCATTC TGTTTACCTG TGCTGTGTCA GGACCGTGGG ACTACAGCAC 
CCGTCTGTGG CCAGTGCCTT CGGTGCCCTG CTGCTGCTGC TGCTGACAGG ACACATATCC 
TACCTGAGCC TTGTCCATTT CGACTACGGC TACAACATGA TGGCCAACGT GGCTATCGGC 
CTGGTGAACC TGGCGTGGTG GCTGGTCTGG TGCCTACGGA ACCGCCAGCG CCTCCCTCAC 
ACCCGCAGGT GTATGGTGGT GGTAGTGCTG CTGCAGGGGC TGTCCCTGCT GGAATTGCTT 
GACTTCCCGC CCCTCTTCTG GGTCCTGGAC GCCCACGCCA TCTGGCACAT CAGCACCATC 
CCGGTCCACA CCCTCTTTTT CAGGTTTCTG GAAGATGACA GCTTGTACCT ACTGAAGGAA 
TCTGGAGCCA TGTTCAAGCT GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533613.1
CDS	93..839
Translation	MWFTVGLYLQEGHRVPQFHGKWPFSRFLFIQEPASAVASLLNGLASLVMLCRYRASVPASSPMYHTCMAFAWVSLNAWFWSTVFHTRDTDLTEKMDYFCASAVILHSVYLCCVRTVGLQHPSVASAFGALLLLLLTGHISYLSLVHFDYGYNMMANVAIGLVNLAWWLVWCLRNRQRLPHTRRCMVVVVLLQGLSLLELLDFPPLFWVLDAHAIWHISTIPVHTLFFRFLEDDSLYLLKESGAMFKLD

Target ORF information:

RefSeq Version	XM_006533550.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus post-GPI attachment to proteins 3 (Pgap3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533550.3

ORF Insert Sequence:

ATGTGGTTCA CTGTTGGCCT CTACCTTCAG GAGGGCCACA GAGTGCCTCA GTTCCATGGC 
AAGTGGCCCT TCTCCCGGTT CCTGTTCATC CAAGAGCCAG CTTCTGCCGT GGCCTCACTC 
CTCAATGGCC TGGCCAGCCT GGTGATGCTC TGCCGCTATC GCGCCTCTGT GCCAGCCTCC 
TCCCCCATGT ACCATACCTG CATGGCCTTC GCTTGGGTGT CTCTCAATGC TTGGTTCTGG 
TCCACAGTCT TCCATACCAG GGACACTGAC CTCACAGAGA AGATGGACTA CTTCTGCGCT 
TCAGCTGTCA TCCTGCATTC TGTTTACCTG TGCTGTGTCA GGACCGTGGG ACTACAGCAC 
CCGTCTGTGG CCAGTGCCTT CGGTGCCCTG CTGCTGCTGC TGCTGACAGG ACACATATCC 
TACCTGAGCC TTGTCCATTT CGACTACGGC TACAACATGA TGGCCAACGT GGCTATCGGC 
CTGGTGAACC TGGCGTGGTG GCTGGTCTGG TGCCTACGGA ACCGCCAGCG CCTCCCTCAC 
ACCCGCAGGT GTATGGTGGT GGTAGTGCTG CTGCAGGGGC TGTCCCTGCT GGAATTGCTT 
GACTTCCCGC CCCTCTTCTG GGTCCTGGAC GCCCACGCCA TCTGGCACAT CAGCACCATC 
CCGGTCCACA CCCTCTTTTT CAGGTTTCTG GAAGATGACA GCTTGTACCT ACTGAAGGAA 
TCTGGAGCCA TGTTCAAGCT GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pgap3 ( XM_006533549.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533612.1 Mus musculus post-GPI attachment to proteins 3 (Pgap3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46472
Clone ID Related Accession (Same CDS sequence)	XM_006533549.3 , XM_006533549.4
Accession Version	XM_006533549.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	post-GPI attachment to proteins factor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533549.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC GGACGGCGCC GCTGCTGCTG CTAACCTTGG CAGTGGGACT CGCGGGGGGT 
TCCCAGGGCG ACCGCGAGCC CGTGTACCGC GACTGCGTGC TCCGATGCGA GGAACGGAAC 
TGTTCGGGAG ACGCACTGAA GCACTTCCGC TCCCGCCAGC CAATCTACAT GAGCCTAGCT 
GGCTGGACCT GTCGGGACGA CTGCAAGTAT GAGTGTATGT GGTTCACTGT TGGCCTCTAC 
CTTCAGGAGG GCCACAGAGT GCCTCAGTTC CATGGCAAGG TGTCTCTCAA TGCTTGGTTC 
TGGTCCACAG TCTTCCATAC CAGGGACACT GACCTCACAG AGAAGATGGA CTACTTCTGC 
GCTTCAGCTG TCATCCTGCA TTCTGTTTAC CTGTGCTGTG TCAGGACCGT GGGACTACAG 
CACCCGTCTG TGGCCAGTGC CTTCGGTGCC CTGCTGCTGC TGCTGCTGAC AGGACACATA 
TCCTACCTGA GCCTTGTCCA TTTCGACTAC GGCTACAACA TGATGGCCAA CGTGGCTATC 
GGCCTGGTGA ACCTGGCGTG GTGGCTGGTC TGGTGCCTAC GGAACCGCCA GCGCCTCCCT 
CACACCCGCA GGTGTATGGT GGTGGTAGTG CTGCTGCAGG GGCTGTCCCT GCTGGAATTG 
CTTGACTTCC CGCCCCTCTT CTGGGTCCTG GACGCCCACG CCATCTGGCA CATCAGCACC 
ATCCCGGTCC ACACCCTCTT TTTCAGGTTT CTGGAAGATG ACAGCTTGTA CCTACTGAAG 
GAATCTGGAG CCATGTTCAA GCTGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533612.1
CDS	802..1611
Translation	MAKRTAPLLLLTLAVGLAGGSQGDREPVYRDCVLRCEERNCSGDALKHFRSRQPIYMSLAGWTCRDDCKYECMWFTVGLYLQEGHRVPQFHGKVSLNAWFWSTVFHTRDTDLTEKMDYFCASAVILHSVYLCCVRTVGLQHPSVASAFGALLLLLLTGHISYLSLVHFDYGYNMMANVAIGLVNLAWWLVWCLRNRQRLPHTRRCMVVVVLLQGLSLLELLDFPPLFWVLDAHAIWHISTIPVHTLFFRFLEDDSLYLLKESGAMFKLD

Target ORF information:

RefSeq Version	XM_006533549.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus post-GPI attachment to proteins 3 (Pgap3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533549.4

ORF Insert Sequence:

ATGGCCAAGC GGACGGCGCC GCTGCTGCTG CTAACCTTGG CAGTGGGACT CGCGGGGGGT 
TCCCAGGGCG ACCGCGAGCC CGTGTACCGC GACTGCGTGC TCCGATGCGA GGAACGGAAC 
TGTTCGGGAG ACGCACTGAA GCACTTCCGC TCCCGCCAGC CAATCTACAT GAGCCTAGCT 
GGCTGGACCT GTCGGGACGA CTGCAAGTAT GAGTGTATGT GGTTCACTGT TGGCCTCTAC 
CTTCAGGAGG GCCACAGAGT GCCTCAGTTC CATGGCAAGG TGTCTCTCAA TGCTTGGTTC 
TGGTCCACAG TCTTCCATAC CAGGGACACT GACCTCACAG AGAAGATGGA CTACTTCTGC 
GCTTCAGCTG TCATCCTGCA TTCTGTTTAC CTGTGCTGTG TCAGGACCGT GGGACTACAG 
CACCCGTCTG TGGCCAGTGC CTTCGGTGCC CTGCTGCTGC TGCTGCTGAC AGGACACATA 
TCCTACCTGA GCCTTGTCCA TTTCGACTAC GGCTACAACA TGATGGCCAA CGTGGCTATC 
GGCCTGGTGA ACCTGGCGTG GTGGCTGGTC TGGTGCCTAC GGAACCGCCA GCGCCTCCCT 
CACACCCGCA GGTGTATGGT GGTGGTAGTG CTGCTGCAGG GGCTGTCCCT GCTGGAATTG 
CTTGACTTCC CGCCCCTCTT CTGGGTCCTG GACGCCCACG CCATCTGGCA CATCAGCACC 
ATCCCGGTCC ACACCCTCTT TTTCAGGTTT CTGGAAGATG ACAGCTTGTA CCTACTGAAG 
GAATCTGGAG CCATGTTCAA GCTGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pgap3 ( XM_006533550.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533613.1 Mus musculus post-GPI attachment to proteins 3 (Pgap3), transcript variant X2, mRNA.

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CloneID	OMu46473
Clone ID Related Accession (Same CDS sequence)	XM_006533550.4 , XM_006533550.3
Accession Version	XM_006533550.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	post-GPI attachment to proteins factor 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533550.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTTCA CTGTTGGCCT CTACCTTCAG GAGGGCCACA GAGTGCCTCA GTTCCATGGC 
AAGTGGCCCT TCTCCCGGTT CCTGTTCATC CAAGAGCCAG CTTCTGCCGT GGCCTCACTC 
CTCAATGGCC TGGCCAGCCT GGTGATGCTC TGCCGCTATC GCGCCTCTGT GCCAGCCTCC 
TCCCCCATGT ACCATACCTG CATGGCCTTC GCTTGGGTGT CTCTCAATGC TTGGTTCTGG 
TCCACAGTCT TCCATACCAG GGACACTGAC CTCACAGAGA AGATGGACTA CTTCTGCGCT 
TCAGCTGTCA TCCTGCATTC TGTTTACCTG TGCTGTGTCA GGACCGTGGG ACTACAGCAC 
CCGTCTGTGG CCAGTGCCTT CGGTGCCCTG CTGCTGCTGC TGCTGACAGG ACACATATCC 
TACCTGAGCC TTGTCCATTT CGACTACGGC TACAACATGA TGGCCAACGT GGCTATCGGC 
CTGGTGAACC TGGCGTGGTG GCTGGTCTGG TGCCTACGGA ACCGCCAGCG CCTCCCTCAC 
ACCCGCAGGT GTATGGTGGT GGTAGTGCTG CTGCAGGGGC TGTCCCTGCT GGAATTGCTT 
GACTTCCCGC CCCTCTTCTG GGTCCTGGAC GCCCACGCCA TCTGGCACAT CAGCACCATC 
CCGGTCCACA CCCTCTTTTT CAGGTTTCTG GAAGATGACA GCTTGTACCT ACTGAAGGAA 
TCTGGAGCCA TGTTCAAGCT GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533613.1
CDS	94..840
Translation	MWFTVGLYLQEGHRVPQFHGKWPFSRFLFIQEPASAVASLLNGLASLVMLCRYRASVPASSPMYHTCMAFAWVSLNAWFWSTVFHTRDTDLTEKMDYFCASAVILHSVYLCCVRTVGLQHPSVASAFGALLLLLLTGHISYLSLVHFDYGYNMMANVAIGLVNLAWWLVWCLRNRQRLPHTRRCMVVVVLLQGLSLLELLDFPPLFWVLDAHAIWHISTIPVHTLFFRFLEDDSLYLLKESGAMFKLD

Target ORF information:

RefSeq Version	XM_006533550.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus post-GPI attachment to proteins 3 (Pgap3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533550.4

ORF Insert Sequence:

ATGTGGTTCA CTGTTGGCCT CTACCTTCAG GAGGGCCACA GAGTGCCTCA GTTCCATGGC 
AAGTGGCCCT TCTCCCGGTT CCTGTTCATC CAAGAGCCAG CTTCTGCCGT GGCCTCACTC 
CTCAATGGCC TGGCCAGCCT GGTGATGCTC TGCCGCTATC GCGCCTCTGT GCCAGCCTCC 
TCCCCCATGT ACCATACCTG CATGGCCTTC GCTTGGGTGT CTCTCAATGC TTGGTTCTGG 
TCCACAGTCT TCCATACCAG GGACACTGAC CTCACAGAGA AGATGGACTA CTTCTGCGCT 
TCAGCTGTCA TCCTGCATTC TGTTTACCTG TGCTGTGTCA GGACCGTGGG ACTACAGCAC 
CCGTCTGTGG CCAGTGCCTT CGGTGCCCTG CTGCTGCTGC TGCTGACAGG ACACATATCC 
TACCTGAGCC TTGTCCATTT CGACTACGGC TACAACATGA TGGCCAACGT GGCTATCGGC 
CTGGTGAACC TGGCGTGGTG GCTGGTCTGG TGCCTACGGA ACCGCCAGCG CCTCCCTCAC 
ACCCGCAGGT GTATGGTGGT GGTAGTGCTG CTGCAGGGGC TGTCCCTGCT GGAATTGCTT 
GACTTCCCGC CCCTCTTCTG GGTCCTGGAC GCCCACGCCA TCTGGCACAT CAGCACCATC 
CCGGTCCACA CCCTCTTTTT CAGGTTTCTG GAAGATGACA GCTTGTACCT ACTGAAGGAA 
TCTGGAGCCA TGTTCAAGCT GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Significance of glycosylphosphatidylinositol-anchored protein enrichment in lipid rafts for the control of autoimmunity.
The Journal of biological chemistry288(35)25490-9(2013 Aug)
Wang Y,Murakami Y,Yasui T,Wakana S,Kikutani H,Kinoshita T,Maeda Y

Enhanced response of T lymphocytes from Pgap3 knockout mouse: Insight into roles of fatty acid remodeling of GPI anchored proteins.
Biochemical and biophysical research communications417(4)1235-41(2012 Jan)
Murakami H,Wang Y,Hasuwa H,Maeda Y,Kinoshita T,Murakami Y

A mouse knockout library for secreted and transmembrane proteins.
Nature biotechnology28(7)749-55(2010 Jul)
Tang T,Li L,Tang J,Li Y,Lin WY,Martin F,Grant D,Solloway M,Parker L,Ye W,Forrest W,Ghilardi N,Oravecz T,Platt KA,Rice DS,Hansen GM,Abuin A,Eberhart DE,Godowski P,Holt KH,Peterson A,Zambrowicz BP,de Sauvage FJ

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Pgap3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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