Dsel cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dsel
Entrez Gene ID	319901
Full Name	dermatan sulfate epimerase-like
Synonyms	9330132E09Rik,DS-epi2
General protein information	Preferred Names dermatan sulfate epimerase-like Names dermatan-sulfate epimerase-like protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 E2.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017321184.1	XP_017176673.1	dermatan-sulfate epimerase-like protein isoform X1
NM_001081316.1	NP_001074785.1	dermatan-sulfate epimerase-like protein precursor
XM_030254948.1	XP_030110808.1	dermatan-sulfate epimerase-like protein isoform X1
XM_017321183.1	XP_017176672.1	dermatan-sulfate epimerase-like protein isoform X1
XM_017321183.2	XP_017176672.1	dermatan-sulfate epimerase-like protein isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DSEL	NP_115536.1
Rattus norvegicus (Norway rat)	Dsel	XP_006249670.1
Danio rerio (zebrafish)	dsela	XP_696502.2
Pan troglodytes (chimpanzee)	DSEL	XP_523962.1
	DSEL	XP_001098463.1
Gallus gallus (chicken)	DSEL	XP_004935280.1
Bos taurus (cattle)	DSEL	NP_001193390.1
Mus musculus (house mouse)	Dsel	NP_001074785.1
Canis lupus familiaris (dog)	DSEL	XP_541064.2
Xenopus tropicalis (tropical clawed frog)	dsel	XP_002939660.1

Gene Ontology
Bibliography

Related articles in PubMed

Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis.
Stachtea XN, Tykesson E, van Kuppevelt TH, Feinstein R, Malmstr?m A, Reijmers RM, Maccarana M
PloS one10(10)e0140279(2015)

Biological functions of iduronic acid in chondroitin/dermatan sulfate.
Thelin MA, Bartolini B, Axelsson J, Gustafsson R, Tykesson E, Pera E, Oldberg ?, Maccarana M, Malmstrom A
The FEBS journal280(10)2431-46(2013 May)

Iduronic acid in chondroitin/dermatan sulfate affects directional migration of aortic smooth muscle cells.
Bartolini B, Thelin MA, Svensson L, Ghiselli G, van Kuppevelt TH, Malmstr?m A, Maccarana M
PloS one8(7)e66704(2013)

Mouse development is not obviously affected by the absence of dermatan sulfate epimerase 2 in spite of a modified brain dermatan sulfate composition.
Bartolini B, Thelin MA, Rauch U, Feinstein R, Oldberg A, Malmstr?m A, Maccarana M
Glycobiology22(7)1007-16(2012 Jul)

Dermatan sulfate epimerase 2 is the predominant isozyme in the formation of the chondroitin sulfate/dermatan sulfate hybrid structure in postnatal developing mouse brain.
Akatsu C, Mizumoto S, Kaneiwa T, Maccarana M, Malmstr?m A, Yamada S, Sugahara K
Glycobiology21(5)565-74(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that dermatan sulfate is completely absent in dermatan sulfate epimerase 1 and 2 double knockout (DKO) mice.
Title: Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis.

DS-epi1 deficiency results in a major reduction of iduronic acid both on cell surface and in secreted CS/DS proteoglycans and affects directional migration of aortic smooth muscle cells.
Title: Iduronic acid in chondroitin/dermatan sulfate affects directional migration of aortic smooth muscle cells.

DS-epi2-deficient mice showed no anatomical, histological or morphological abnormalities and DS-epi1 likely compensates in vivo for the loss of DS-epi2
Title: Mouse development is not obviously affected by the absence of dermatan sulfate epimerase 2 in spite of a modified brain dermatan sulfate composition.

the iduronic acid-containing structures in the developing brain are mainly produced by the actions of dermatan sulfate epimerase 2 and play crucial roles in postnatal development
Title: Dermatan sulfate epimerase 2 is the predominant isozyme in the formation of the chondroitin sulfate/dermatan sulfate hybrid structure in postnatal developing mouse brain.

DSEL was selected for study in 125 patients with diaphragmatic hernias, as it is involved in the synthesis of decorin, a protein that is required for normal collagen formation and that is upregulated during myogenesis.
Title: A maternally inherited chromosome 18q22.1 deletion in a male with late-presenting diaphragmatic hernia and microphthalmia-evaluation of DSEL as a candidate gene for the diaphragmatic defect.

The following Dsel gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dsel cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02102	XM_017321183.2 Latest version!	Mus musculus dermatan sulfate epimerase-like (Dsel), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu02102	XM_030254948.1 Latest version!	Mus musculus dermatan sulfate epimerase-like (Dsel), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu02102	NM_001081316.1 Latest version!	Mus musculus dermatan sulfate epimerase-like (Dsel), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu02102	XM_017321184.1 Latest version!	Mus musculus dermatan sulfate epimerase-like (Dsel), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu02102	XM_017321183.1	Mus musculus dermatan sulfate epimerase-like (Dsel), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dsel ( XM_030254948.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110808.1 Mus musculus dermatan sulfate epimerase-like (Dsel), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu02102
Clone ID Related Accession (Same CDS sequence)	XM_017321184.1 , NM_001081316.1 , XM_030254948.1 , XM_017321183.1 , XM_017321183.2
Accession Version	XM_030254948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	dermatan-sulfate epimerase-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTTA TGTTTACAGA ACATTTACTA TTTTTAACAT TGATGATGTG TAGTTTTTCT 
ACTTGTGAAG AATCTGTGAG CAATTATTCT GAATGGGCAG TTTTCACAGA CGATATACAA 
TGGCTTAAGT CACAGAAAAT ACAAGATTTC AAACTCAACC GAAGACTTCA TCCAAATTTA 
TATTTTGATG CTGGAGATAT ACAAACATTG AAACAAAAGT CTCGTACAAG CCATTTGCAT 
ATTTTTAGAG CTATCAAAAG TGCAGTGACA ATTATGCTGT CCAATCCATC ATACTACCTA 
CCTCCACCCA AGCATGCTGA GTTTGCTGCC AAGTGGAATG AAATTTATGG TAATAATCTT 
CCTCCTTTAG CATTGTATTG TTTATTATGC CCAGAAGACA AGGTTGCCTT TGAATTTGTT 
ATGGAATACA TGGATCGGAT GGTTAGCTAC AAAGACTGGC TAGTTGAGAA TGCACCAGGG 
GATGAGGTTC CAGTTGGCCA TTCTTTAACA GGTTTTGCCA CTGCCTTTGA CTTTTTATAT 
AATCTATTAG GTAATCAGCG TAAACAAAAA TACCTAGAAA AAATTTGGAT TGTTACTGAG 
GAAATGTATG AATATTCCAA GATTCGATCA TGGGGCAAAC AACTTCTTCA TAACCATCAA 
GCTACAAATA TGATAGCTTT ACTCATAGGG GCCTTGGTTA CTGGAGTAGA TAAAGGATCT 
AAAGCAAACA TATGGAAACA AGTTGTTGTT GATGTGATGG AAAAGACTAT GTTTCTCTTG 
AAGCATATTG TAGATGGCTC ATTGGATGAA GGTGTGGCCT ATGGAAGCTA TACCTCAAAA 
TCAGTTACAC AGTATGTTTT TTTGGCACAA CGCCATTTTA ACATCAACAA CTTTGATAAT 
AACTGGCTAA AAATGCATTT TTGGTTTTAT TATGCTACAC TTTTGCCAGG CTATCAAAGA 
ACTGTAGGCA TAGCAGATTC CAATTATAAT TGGTTTTATG GTCCAGAGAG CCAGCTAGTT 
TTCTTGGATA AGTTCATTTT ACAGAATGGA GCTGGAAATT GGTTAGCTCA GCAAATTAGA 
AAGCATCGAC CTAAGGATGG ACCAATGGTT CCTTCCACTG CTCAGCGGTG GAGTACTCTT 
CATACTGAAT ACATCTGGTA TGATCCAACA CTCACCCCAC AGCCTCCTGT TGATTTTGGC 
ACTGCAAAAA TGCACACATT TCCTAACTGG GGTGTCGTGA CTTATGGGGG TGGGCTGCCA 
AACACCCAGA CCAATACCTT TGTGTCTTTT AAATCTGGGA AACTGGGAGG ACGAGCTGTG 
TATGACATAG TTCACTTTCA GCCATATTCC TGGATTGATG GATGGAGAAG CTTTAACCCA 
GGACATGAAC ATCCAGATCA AAATTCATTT ACTTTCGCTC CTAATGGGCA GGTATTCGTT 
TCTGAGGCTC TTTATGGACC AAAATTGAGC CACCTTAACA ACGTATTGGT GTTTGCCCCA 
TCACCATCAA GTCAATGTAA TCAGCCCTGG GAAGGTCAAC TGGGAGAATG TGCACAGTGG 
CTCAAGTGGA CTGGGGAAGA GGTTGGTGAT GCAGCTGGGG AAGTTATTAC TGCTGCTCAA 
CATGGTGATA GGATGTTTGT GAGTGGGGAA GCAGTGTCTG CTTATTCTTC TGCCATGAGA 
CTGAAAAGTG TCTATCGTGC TTTACTTCTT TTAAATTCAC AAACTCTGCT TGTTGTCGAT 
CATATTGAAA GGCAAGAAAC TTCCCCAATA AATTCTGTCA GTGCCTTCTT TCATAATTTG 
GATATTGATT TTAAATACAT CCCATACAAG TTTATGAATA GATATAATGG TGCCATGATG 
GATGTGTGGG ATGCACACTA TAAAATGTTT TGGTTTGATC ACCATGGCAA CAGTCCTGTG 
GCTAATATAC AGGAAGCAGA ACAGGCTGCT GAATTTAAGA AACGGTGGAC ACAGTTTGTT 
AATGTTACAT TTCATATGGA ATCCACAATC ACAAGAATTG CTTATGTATT TTATGGGCCA 
TATGTCAATG TTTCCAGCTG CAGATTTATT GATAGTTCCA GTTCTGGACT TCAGATTTCT 
TTACATGTCA ACAGTACTGA ACATAGTGTG TCTGTTGTAA CTGACTATCA AAACCTTAAA 
AGCAGATTCA GTTACCTGGG ATTTGGTGGT TTTGCCAGTG TGGCTAATCA AGGACAGATA 
ACCAGATTTG GTTTGGGTAC TCAAGAAATA GTAAACCCTG TAAGACATGA TAAAGTTAAT 
TTCCCCTTTG GGTTTAAATT TAATATAGCA GTTGGATTCA TTTTGTGTAT TAGTTTGGTT 
ATTTTAACTT TTCAATGGCG GTTTTACCTT TCCTTTAGAA AGCTAATGCG CTGTGTATTA 
ATACTTGTTA TTGCCTTGTG GTTTATTGAG CTTCTGGATG TATGGAGTAC ATGCACTCAG 
CCCATCTGTG CAAAATGGAC AAGGACTGAA GCTAAGGCAA ATGAGAAGGT CATGATTTCT 
GAAGGGCATC ATGTGGATCT TCCTAATGTT ATTATTACCT CACTCCCTGG TTCAGGAGCT 
GAAATTCTCA AACAGCTTTT TTTCAACAGC AGTGATTTTC TCTACATCAG AATTCCTACA 
GCCTACATGG ATATCCCTGA AACTGAATTT GAAATTGACT CATTTGTAGA TGCTTGTGAG 
TGGAAAGTAT CAGATATCCG CAGTGGGCAC TTTCATCTTC TTCGAGGGTG GCTGCAGTCT 
TTGGTCCAGG ATACAAAACT TCACTTGCAA AACATCCATC TACATGAAAC CAGTAGGAGT 
AAACTGGCCC AATATTTTAC AACTAATAAG GACAAAAAGC GAAAATTAAA AAGAAGGGAG 
TCTTTGCAAG ATCAAAGAAG TAGAATAAAA GGACCATTTG ATAGAGATGC TGAATATATT 
AGGGCTTTAA GAAGACACCT TGTTTATTAC CCAAGTGCAC GTCCTGTGCT CAGCTTAAGT 
AGTGGTAGCT GGACATTGAA GCTTCATTTT TTTCAGGAAG TTTTAGGAAC TTCAATGCGG 
GCATTGTACA TAGTAAGAGA CCCTCGAGCT TGGATCTATT CAGTGCTATA TGGTAGTAAA 
CCAAGTCTTT ATTCTTTGAA GAATGTACCA GAGCACTTAG CAAAATTGTT TAAAATAGAG 
GAAGGTAAAA GCAAATGTAA TTCGAATTCT GGCTATGCTT TTGAGTATGA ATCACTGAAG 
AAAGAATTAG AAATATCCCA ATCAAATGCT ATCTCCTTAT TATCTCATTT GTGGGTAGCA 
AACACTGCAG CAGCCTTGAG AATAAATACA GATTTGCTGC CTACCAATTA CCATCTGGTC 
AAGTTTGAAG ATATTGTTCA TTTTCCTCAG AAGACTACTG AAAGGATTTT TGCTTTCCTT 
GGCATTCCTT TGTCTCCTGC TAGTTTAAAC CAAATGCTAT TTGCCACTTC CACAAACCTT 
TTTTATCTTC CATATGAGGG GGAAATATCA CCATCTAATA CTAATATTTG GAAAACAAAC 
TTGCCTAGAG ATGAAATTAA ACTAATTGAA AACATTTGCT GGACACTGAT GGATCATCTA 
GGATATCCAA AGTTTATGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110808.1
CDS	1701..5324
Translation	MAFMFTEHLLFLTLMMCSFSTCEESVSNYSEWAVFTDDIQWLKSQKIQDFKLNRRLHPNLYFDAGDIQTLKQKSRTSHLHIFRAIKSAVTIMLSNPSYYLPPPKHAEFAAKWNEIYGNNLPPLALYCLLCPEDKVAFEFVMEYMDRMVSYKDWLVENAPGDEVPVGHSLTGFATAFDFLYNLLGNQRKQKYLEKIWIVTEEMYEYSKIRSWGKQLLHNHQATNMIALLIGALVTGVDKGSKANIWKQVVVDVMEKTMFLLKHIVDGSLDEGVAYGSYTSKSVTQYVFLAQRHFNINNFDNNWLKMHFWFYYATLLPGYQRTVGIADSNYNWFYGPESQLVFLDKFILQNGAGNWLAQQIRKHRPKDGPMVPSTAQRWSTLHTEYIWYDPTLTPQPPVDFGTAKMHTFPNWGVVTYGGGLPNTQTNTFVSFKSGKLGGRAVYDIVHFQPYSWIDGWRSFNPGHEHPDQNSFTFAPNGQVFVSEALYGPKLSHLNNVLVFAPSPSSQCNQPWEGQLGECAQWLKWTGEEVGDAAGEVITAAQHGDRMFVSGEAVSAYSSAMRLKSVYRALLLLNSQTLLVVDHIERQETSPINSVSAFFHNLDIDFKYIPYKFMNRYNGAMMDVWDAHYKMFWFDHHGNSPVANIQEAEQAAEFKKRWTQFVNVTFHMESTITRIAYVFYGPYVNVSSCRFIDSSSSGLQISLHVNSTEHSVSVVTDYQNLKSRFSYLGFGGFASVANQGQITRFGLGTQEIVNPVRHDKVNFPFGFKFNIAVGFILCISLVILTFQWRFYLSFRKLMRCVLILVIALWFIELLDVWSTCTQPICAKWTRTEAKANEKVMISEGHHVDLPNVIITSLPGSGAEILKQLFFNSSDFLYIRIPTAYMDIPETEFEIDSFVDACEWKVSDIRSGHFHLLRGWLQSLVQDTKLHLQNIHLHETSRSKLAQYFTTNKDKKRKLKRRESLQDQRSRIKGPFDRDAEYIRALRRHLVYYPSARPVLSLSSGSWTLKLHFFQEVLGTSMRALYIVRDPRAWIYSVLYGSKPSLYSLKNVPEHLAKLFKIEEGKSKCNSNSGYAFEYESLKKELEISQSNAISLLSHLWVANTAAALRINTDLLPTNYHLVKFEDIVHFPQKTTERIFAFLGIPLSPASLNQMLFATSTNLFYLPYEGEISPSNTNIWKTNLPRDEIKLIENICWTLMDHLGYPKFMD

Target ORF information:

RefSeq Version	XM_030254948.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dermatan sulfate epimerase-like (Dsel), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254948.1

ORF Insert Sequence:

ATGGCGTTTA TGTTTACAGA ACATTTACTA TTTTTAACAT TGATGATGTG TAGTTTTTCT 
ACTTGTGAAG AATCTGTGAG CAATTATTCT GAATGGGCAG TTTTCACAGA CGATATACAA 
TGGCTTAAGT CACAGAAAAT ACAAGATTTC AAACTCAACC GAAGACTTCA TCCAAATTTA 
TATTTTGATG CTGGAGATAT ACAAACATTG AAACAAAAGT CTCGTACAAG CCATTTGCAT 
ATTTTTAGAG CTATCAAAAG TGCAGTGACA ATTATGCTGT CCAATCCATC ATACTACCTA 
CCTCCACCCA AGCATGCTGA GTTTGCTGCC AAGTGGAATG AAATTTATGG TAATAATCTT 
CCTCCTTTAG CATTGTATTG TTTATTATGC CCAGAAGACA AGGTTGCCTT TGAATTTGTT 
ATGGAATACA TGGATCGGAT GGTTAGCTAC AAAGACTGGC TAGTTGAGAA TGCACCAGGG 
GATGAGGTTC CAGTTGGCCA TTCTTTAACA GGTTTTGCCA CTGCCTTTGA CTTTTTATAT 
AATCTATTAG GTAATCAGCG TAAACAAAAA TACCTAGAAA AAATTTGGAT TGTTACTGAG 
GAAATGTATG AATATTCCAA GATTCGATCA TGGGGCAAAC AACTTCTTCA TAACCATCAA 
GCTACAAATA TGATAGCTTT ACTCATAGGG GCCTTGGTTA CTGGAGTAGA TAAAGGATCT 
AAAGCAAACA TATGGAAACA AGTTGTTGTT GATGTGATGG AAAAGACTAT GTTTCTCTTG 
AAGCATATTG TAGATGGCTC ATTGGATGAA GGTGTGGCCT ATGGAAGCTA TACCTCAAAA 
TCAGTTACAC AGTATGTTTT TTTGGCACAA CGCCATTTTA ACATCAACAA CTTTGATAAT 
AACTGGCTAA AAATGCATTT TTGGTTTTAT TATGCTACAC TTTTGCCAGG CTATCAAAGA 
ACTGTAGGCA TAGCAGATTC CAATTATAAT TGGTTTTATG GTCCAGAGAG CCAGCTAGTT 
TTCTTGGATA AGTTCATTTT ACAGAATGGA GCTGGAAATT GGTTAGCTCA GCAAATTAGA 
AAGCATCGAC CTAAGGATGG ACCAATGGTT CCTTCCACTG CTCAGCGGTG GAGTACTCTT 
CATACTGAAT ACATCTGGTA TGATCCAACA CTCACCCCAC AGCCTCCTGT TGATTTTGGC 
ACTGCAAAAA TGCACACATT TCCTAACTGG GGTGTCGTGA CTTATGGGGG TGGGCTGCCA 
AACACCCAGA CCAATACCTT TGTGTCTTTT AAATCTGGGA AACTGGGAGG ACGAGCTGTG 
TATGACATAG TTCACTTTCA GCCATATTCC TGGATTGATG GATGGAGAAG CTTTAACCCA 
GGACATGAAC ATCCAGATCA AAATTCATTT ACTTTCGCTC CTAATGGGCA GGTATTCGTT 
TCTGAGGCTC TTTATGGACC AAAATTGAGC CACCTTAACA ACGTATTGGT GTTTGCCCCA 
TCACCATCAA GTCAATGTAA TCAGCCCTGG GAAGGTCAAC TGGGAGAATG TGCACAGTGG 
CTCAAGTGGA CTGGGGAAGA GGTTGGTGAT GCAGCTGGGG AAGTTATTAC TGCTGCTCAA 
CATGGTGATA GGATGTTTGT GAGTGGGGAA GCAGTGTCTG CTTATTCTTC TGCCATGAGA 
CTGAAAAGTG TCTATCGTGC TTTACTTCTT TTAAATTCAC AAACTCTGCT TGTTGTCGAT 
CATATTGAAA GGCAAGAAAC TTCCCCAATA AATTCTGTCA GTGCCTTCTT TCATAATTTG 
GATATTGATT TTAAATACAT CCCATACAAG TTTATGAATA GATATAATGG TGCCATGATG 
GATGTGTGGG ATGCACACTA TAAAATGTTT TGGTTTGATC ACCATGGCAA CAGTCCTGTG 
GCTAATATAC AGGAAGCAGA ACAGGCTGCT GAATTTAAGA AACGGTGGAC ACAGTTTGTT 
AATGTTACAT TTCATATGGA ATCCACAATC ACAAGAATTG CTTATGTATT TTATGGGCCA 
TATGTCAATG TTTCCAGCTG CAGATTTATT GATAGTTCCA GTTCTGGACT TCAGATTTCT 
TTACATGTCA ACAGTACTGA ACATAGTGTG TCTGTTGTAA CTGACTATCA AAACCTTAAA 
AGCAGATTCA GTTACCTGGG ATTTGGTGGT TTTGCCAGTG TGGCTAATCA AGGACAGATA 
ACCAGATTTG GTTTGGGTAC TCAAGAAATA GTAAACCCTG TAAGACATGA TAAAGTTAAT 
TTCCCCTTTG GGTTTAAATT TAATATAGCA GTTGGATTCA TTTTGTGTAT TAGTTTGGTT 
ATTTTAACTT TTCAATGGCG GTTTTACCTT TCCTTTAGAA AGCTAATGCG CTGTGTATTA 
ATACTTGTTA TTGCCTTGTG GTTTATTGAG CTTCTGGATG TATGGAGTAC ATGCACTCAG 
CCCATCTGTG CAAAATGGAC AAGGACTGAA GCTAAGGCAA ATGAGAAGGT CATGATTTCT 
GAAGGGCATC ATGTGGATCT TCCTAATGTT ATTATTACCT CACTCCCTGG TTCAGGAGCT 
GAAATTCTCA AACAGCTTTT TTTCAACAGC AGTGATTTTC TCTACATCAG AATTCCTACA 
GCCTACATGG ATATCCCTGA AACTGAATTT GAAATTGACT CATTTGTAGA TGCTTGTGAG 
TGGAAAGTAT CAGATATCCG CAGTGGGCAC TTTCATCTTC TTCGAGGGTG GCTGCAGTCT 
TTGGTCCAGG ATACAAAACT TCACTTGCAA AACATCCATC TACATGAAAC CAGTAGGAGT 
AAACTGGCCC AATATTTTAC AACTAATAAG GACAAAAAGC GAAAATTAAA AAGAAGGGAG 
TCTTTGCAAG ATCAAAGAAG TAGAATAAAA GGACCATTTG ATAGAGATGC TGAATATATT 
AGGGCTTTAA GAAGACACCT TGTTTATTAC CCAAGTGCAC GTCCTGTGCT CAGCTTAAGT 
AGTGGTAGCT GGACATTGAA GCTTCATTTT TTTCAGGAAG TTTTAGGAAC TTCAATGCGG 
GCATTGTACA TAGTAAGAGA CCCTCGAGCT TGGATCTATT CAGTGCTATA TGGTAGTAAA 
CCAAGTCTTT ATTCTTTGAA GAATGTACCA GAGCACTTAG CAAAATTGTT TAAAATAGAG 
GAAGGTAAAA GCAAATGTAA TTCGAATTCT GGCTATGCTT TTGAGTATGA ATCACTGAAG 
AAAGAATTAG AAATATCCCA ATCAAATGCT ATCTCCTTAT TATCTCATTT GTGGGTAGCA 
AACACTGCAG CAGCCTTGAG AATAAATACA GATTTGCTGC CTACCAATTA CCATCTGGTC 
AAGTTTGAAG ATATTGTTCA TTTTCCTCAG AAGACTACTG AAAGGATTTT TGCTTTCCTT 
GGCATTCCTT TGTCTCCTGC TAGTTTAAAC CAAATGCTAT TTGCCACTTC CACAAACCTT 
TTTTATCTTC CATATGAGGG GGAAATATCA CCATCTAATA CTAATATTTG GAAAACAAAC 
TTGCCTAGAG ATGAAATTAA ACTAATTGAA AACATTTGCT GGACACTGAT GGATCATCTA 
GGATATCCAA AGTTTATGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsel ( XM_017321183.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176672.1 Mus musculus dermatan sulfate epimerase-like (Dsel), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu02102
Clone ID Related Accession (Same CDS sequence)	XM_017321184.1 , NM_001081316.1 , XM_030254948.1 , XM_017321183.1 , XM_017321183.2
Accession Version	XM_017321183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	dermatan-sulfate epimerase-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTTA TGTTTACAGA ACATTTACTA TTTTTAACAT TGATGATGTG TAGTTTTTCT 
ACTTGTGAAG AATCTGTGAG CAATTATTCT GAATGGGCAG TTTTCACAGA CGATATACAA 
TGGCTTAAGT CACAGAAAAT ACAAGATTTC AAACTCAACC GAAGACTTCA TCCAAATTTA 
TATTTTGATG CTGGAGATAT ACAAACATTG AAACAAAAGT CTCGTACAAG CCATTTGCAT 
ATTTTTAGAG CTATCAAAAG TGCAGTGACA ATTATGCTGT CCAATCCATC ATACTACCTA 
CCTCCACCCA AGCATGCTGA GTTTGCTGCC AAGTGGAATG AAATTTATGG TAATAATCTT 
CCTCCTTTAG CATTGTATTG TTTATTATGC CCAGAAGACA AGGTTGCCTT TGAATTTGTT 
ATGGAATACA TGGATCGGAT GGTTAGCTAC AAAGACTGGC TAGTTGAGAA TGCACCAGGG 
GATGAGGTTC CAGTTGGCCA TTCTTTAACA GGTTTTGCCA CTGCCTTTGA CTTTTTATAT 
AATCTATTAG GTAATCAGCG TAAACAAAAA TACCTAGAAA AAATTTGGAT TGTTACTGAG 
GAAATGTATG AATATTCCAA GATTCGATCA TGGGGCAAAC AACTTCTTCA TAACCATCAA 
GCTACAAATA TGATAGCTTT ACTCATAGGG GCCTTGGTTA CTGGAGTAGA TAAAGGATCT 
AAAGCAAACA TATGGAAACA AGTTGTTGTT GATGTGATGG AAAAGACTAT GTTTCTCTTG 
AAGCATATTG TAGATGGCTC ATTGGATGAA GGTGTGGCCT ATGGAAGCTA TACCTCAAAA 
TCAGTTACAC AGTATGTTTT TTTGGCACAA CGCCATTTTA ACATCAACAA CTTTGATAAT 
AACTGGCTAA AAATGCATTT TTGGTTTTAT TATGCTACAC TTTTGCCAGG CTATCAAAGA 
ACTGTAGGCA TAGCAGATTC CAATTATAAT TGGTTTTATG GTCCAGAGAG CCAGCTAGTT 
TTCTTGGATA AGTTCATTTT ACAGAATGGA GCTGGAAATT GGTTAGCTCA GCAAATTAGA 
AAGCATCGAC CTAAGGATGG ACCAATGGTT CCTTCCACTG CTCAGCGGTG GAGTACTCTT 
CATACTGAAT ACATCTGGTA TGATCCAACA CTCACCCCAC AGCCTCCTGT TGATTTTGGC 
ACTGCAAAAA TGCACACATT TCCTAACTGG GGTGTCGTGA CTTATGGGGG TGGGCTGCCA 
AACACCCAGA CCAATACCTT TGTGTCTTTT AAATCTGGGA AACTGGGAGG ACGAGCTGTG 
TATGACATAG TTCACTTTCA GCCATATTCC TGGATTGATG GATGGAGAAG CTTTAACCCA 
GGACATGAAC ATCCAGATCA AAATTCATTT ACTTTCGCTC CTAATGGGCA GGTATTCGTT 
TCTGAGGCTC TTTATGGACC AAAATTGAGC CACCTTAACA ACGTATTGGT GTTTGCCCCA 
TCACCATCAA GTCAATGTAA TCAGCCCTGG GAAGGTCAAC TGGGAGAATG TGCACAGTGG 
CTCAAGTGGA CTGGGGAAGA GGTTGGTGAT GCAGCTGGGG AAGTTATTAC TGCTGCTCAA 
CATGGTGATA GGATGTTTGT GAGTGGGGAA GCAGTGTCTG CTTATTCTTC TGCCATGAGA 
CTGAAAAGTG TCTATCGTGC TTTACTTCTT TTAAATTCAC AAACTCTGCT TGTTGTCGAT 
CATATTGAAA GGCAAGAAAC TTCCCCAATA AATTCTGTCA GTGCCTTCTT TCATAATTTG 
GATATTGATT TTAAATACAT CCCATACAAG TTTATGAATA GATATAATGG TGCCATGATG 
GATGTGTGGG ATGCACACTA TAAAATGTTT TGGTTTGATC ACCATGGCAA CAGTCCTGTG 
GCTAATATAC AGGAAGCAGA ACAGGCTGCT GAATTTAAGA AACGGTGGAC ACAGTTTGTT 
AATGTTACAT TTCATATGGA ATCCACAATC ACAAGAATTG CTTATGTATT TTATGGGCCA 
TATGTCAATG TTTCCAGCTG CAGATTTATT GATAGTTCCA GTTCTGGACT TCAGATTTCT 
TTACATGTCA ACAGTACTGA ACATAGTGTG TCTGTTGTAA CTGACTATCA AAACCTTAAA 
AGCAGATTCA GTTACCTGGG ATTTGGTGGT TTTGCCAGTG TGGCTAATCA AGGACAGATA 
ACCAGATTTG GTTTGGGTAC TCAAGAAATA GTAAACCCTG TAAGACATGA TAAAGTTAAT 
TTCCCCTTTG GGTTTAAATT TAATATAGCA GTTGGATTCA TTTTGTGTAT TAGTTTGGTT 
ATTTTAACTT TTCAATGGCG GTTTTACCTT TCCTTTAGAA AGCTAATGCG CTGTGTATTA 
ATACTTGTTA TTGCCTTGTG GTTTATTGAG CTTCTGGATG TATGGAGTAC ATGCACTCAG 
CCCATCTGTG CAAAATGGAC AAGGACTGAA GCTAAGGCAA ATGAGAAGGT CATGATTTCT 
GAAGGGCATC ATGTGGATCT TCCTAATGTT ATTATTACCT CACTCCCTGG TTCAGGAGCT 
GAAATTCTCA AACAGCTTTT TTTCAACAGC AGTGATTTTC TCTACATCAG AATTCCTACA 
GCCTACATGG ATATCCCTGA AACTGAATTT GAAATTGACT CATTTGTAGA TGCTTGTGAG 
TGGAAAGTAT CAGATATCCG CAGTGGGCAC TTTCATCTTC TTCGAGGGTG GCTGCAGTCT 
TTGGTCCAGG ATACAAAACT TCACTTGCAA AACATCCATC TACATGAAAC CAGTAGGAGT 
AAACTGGCCC AATATTTTAC AACTAATAAG GACAAAAAGC GAAAATTAAA AAGAAGGGAG 
TCTTTGCAAG ATCAAAGAAG TAGAATAAAA GGACCATTTG ATAGAGATGC TGAATATATT 
AGGGCTTTAA GAAGACACCT TGTTTATTAC CCAAGTGCAC GTCCTGTGCT CAGCTTAAGT 
AGTGGTAGCT GGACATTGAA GCTTCATTTT TTTCAGGAAG TTTTAGGAAC TTCAATGCGG 
GCATTGTACA TAGTAAGAGA CCCTCGAGCT TGGATCTATT CAGTGCTATA TGGTAGTAAA 
CCAAGTCTTT ATTCTTTGAA GAATGTACCA GAGCACTTAG CAAAATTGTT TAAAATAGAG 
GAAGGTAAAA GCAAATGTAA TTCGAATTCT GGCTATGCTT TTGAGTATGA ATCACTGAAG 
AAAGAATTAG AAATATCCCA ATCAAATGCT ATCTCCTTAT TATCTCATTT GTGGGTAGCA 
AACACTGCAG CAGCCTTGAG AATAAATACA GATTTGCTGC CTACCAATTA CCATCTGGTC 
AAGTTTGAAG ATATTGTTCA TTTTCCTCAG AAGACTACTG AAAGGATTTT TGCTTTCCTT 
GGCATTCCTT TGTCTCCTGC TAGTTTAAAC CAAATGCTAT TTGCCACTTC CACAAACCTT 
TTTTATCTTC CATATGAGGG GGAAATATCA CCATCTAATA CTAATATTTG GAAAACAAAC 
TTGCCTAGAG ATGAAATTAA ACTAATTGAA AACATTTGCT GGACACTGAT GGATCATCTA 
GGATATCCAA AGTTTATGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176672.1
CDS	1289..4912
Translation	MAFMFTEHLLFLTLMMCSFSTCEESVSNYSEWAVFTDDIQWLKSQKIQDFKLNRRLHPNLYFDAGDIQTLKQKSRTSHLHIFRAIKSAVTIMLSNPSYYLPPPKHAEFAAKWNEIYGNNLPPLALYCLLCPEDKVAFEFVMEYMDRMVSYKDWLVENAPGDEVPVGHSLTGFATAFDFLYNLLGNQRKQKYLEKIWIVTEEMYEYSKIRSWGKQLLHNHQATNMIALLIGALVTGVDKGSKANIWKQVVVDVMEKTMFLLKHIVDGSLDEGVAYGSYTSKSVTQYVFLAQRHFNINNFDNNWLKMHFWFYYATLLPGYQRTVGIADSNYNWFYGPESQLVFLDKFILQNGAGNWLAQQIRKHRPKDGPMVPSTAQRWSTLHTEYIWYDPTLTPQPPVDFGTAKMHTFPNWGVVTYGGGLPNTQTNTFVSFKSGKLGGRAVYDIVHFQPYSWIDGWRSFNPGHEHPDQNSFTFAPNGQVFVSEALYGPKLSHLNNVLVFAPSPSSQCNQPWEGQLGECAQWLKWTGEEVGDAAGEVITAAQHGDRMFVSGEAVSAYSSAMRLKSVYRALLLLNSQTLLVVDHIERQETSPINSVSAFFHNLDIDFKYIPYKFMNRYNGAMMDVWDAHYKMFWFDHHGNSPVANIQEAEQAAEFKKRWTQFVNVTFHMESTITRIAYVFYGPYVNVSSCRFIDSSSSGLQISLHVNSTEHSVSVVTDYQNLKSRFSYLGFGGFASVANQGQITRFGLGTQEIVNPVRHDKVNFPFGFKFNIAVGFILCISLVILTFQWRFYLSFRKLMRCVLILVIALWFIELLDVWSTCTQPICAKWTRTEAKANEKVMISEGHHVDLPNVIITSLPGSGAEILKQLFFNSSDFLYIRIPTAYMDIPETEFEIDSFVDACEWKVSDIRSGHFHLLRGWLQSLVQDTKLHLQNIHLHETSRSKLAQYFTTNKDKKRKLKRRESLQDQRSRIKGPFDRDAEYIRALRRHLVYYPSARPVLSLSSGSWTLKLHFFQEVLGTSMRALYIVRDPRAWIYSVLYGSKPSLYSLKNVPEHLAKLFKIEEGKSKCNSNSGYAFEYESLKKELEISQSNAISLLSHLWVANTAAALRINTDLLPTNYHLVKFEDIVHFPQKTTERIFAFLGIPLSPASLNQMLFATSTNLFYLPYEGEISPSNTNIWKTNLPRDEIKLIENICWTLMDHLGYPKFMD

Target ORF information:

RefSeq Version	XM_017321183.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dermatan sulfate epimerase-like (Dsel), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321183.2

ORF Insert Sequence:

ATGGCGTTTA TGTTTACAGA ACATTTACTA TTTTTAACAT TGATGATGTG TAGTTTTTCT 
ACTTGTGAAG AATCTGTGAG CAATTATTCT GAATGGGCAG TTTTCACAGA CGATATACAA 
TGGCTTAAGT CACAGAAAAT ACAAGATTTC AAACTCAACC GAAGACTTCA TCCAAATTTA 
TATTTTGATG CTGGAGATAT ACAAACATTG AAACAAAAGT CTCGTACAAG CCATTTGCAT 
ATTTTTAGAG CTATCAAAAG TGCAGTGACA ATTATGCTGT CCAATCCATC ATACTACCTA 
CCTCCACCCA AGCATGCTGA GTTTGCTGCC AAGTGGAATG AAATTTATGG TAATAATCTT 
CCTCCTTTAG CATTGTATTG TTTATTATGC CCAGAAGACA AGGTTGCCTT TGAATTTGTT 
ATGGAATACA TGGATCGGAT GGTTAGCTAC AAAGACTGGC TAGTTGAGAA TGCACCAGGG 
GATGAGGTTC CAGTTGGCCA TTCTTTAACA GGTTTTGCCA CTGCCTTTGA CTTTTTATAT 
AATCTATTAG GTAATCAGCG TAAACAAAAA TACCTAGAAA AAATTTGGAT TGTTACTGAG 
GAAATGTATG AATATTCCAA GATTCGATCA TGGGGCAAAC AACTTCTTCA TAACCATCAA 
GCTACAAATA TGATAGCTTT ACTCATAGGG GCCTTGGTTA CTGGAGTAGA TAAAGGATCT 
AAAGCAAACA TATGGAAACA AGTTGTTGTT GATGTGATGG AAAAGACTAT GTTTCTCTTG 
AAGCATATTG TAGATGGCTC ATTGGATGAA GGTGTGGCCT ATGGAAGCTA TACCTCAAAA 
TCAGTTACAC AGTATGTTTT TTTGGCACAA CGCCATTTTA ACATCAACAA CTTTGATAAT 
AACTGGCTAA AAATGCATTT TTGGTTTTAT TATGCTACAC TTTTGCCAGG CTATCAAAGA 
ACTGTAGGCA TAGCAGATTC CAATTATAAT TGGTTTTATG GTCCAGAGAG CCAGCTAGTT 
TTCTTGGATA AGTTCATTTT ACAGAATGGA GCTGGAAATT GGTTAGCTCA GCAAATTAGA 
AAGCATCGAC CTAAGGATGG ACCAATGGTT CCTTCCACTG CTCAGCGGTG GAGTACTCTT 
CATACTGAAT ACATCTGGTA TGATCCAACA CTCACCCCAC AGCCTCCTGT TGATTTTGGC 
ACTGCAAAAA TGCACACATT TCCTAACTGG GGTGTCGTGA CTTATGGGGG TGGGCTGCCA 
AACACCCAGA CCAATACCTT TGTGTCTTTT AAATCTGGGA AACTGGGAGG ACGAGCTGTG 
TATGACATAG TTCACTTTCA GCCATATTCC TGGATTGATG GATGGAGAAG CTTTAACCCA 
GGACATGAAC ATCCAGATCA AAATTCATTT ACTTTCGCTC CTAATGGGCA GGTATTCGTT 
TCTGAGGCTC TTTATGGACC AAAATTGAGC CACCTTAACA ACGTATTGGT GTTTGCCCCA 
TCACCATCAA GTCAATGTAA TCAGCCCTGG GAAGGTCAAC TGGGAGAATG TGCACAGTGG 
CTCAAGTGGA CTGGGGAAGA GGTTGGTGAT GCAGCTGGGG AAGTTATTAC TGCTGCTCAA 
CATGGTGATA GGATGTTTGT GAGTGGGGAA GCAGTGTCTG CTTATTCTTC TGCCATGAGA 
CTGAAAAGTG TCTATCGTGC TTTACTTCTT TTAAATTCAC AAACTCTGCT TGTTGTCGAT 
CATATTGAAA GGCAAGAAAC TTCCCCAATA AATTCTGTCA GTGCCTTCTT TCATAATTTG 
GATATTGATT TTAAATACAT CCCATACAAG TTTATGAATA GATATAATGG TGCCATGATG 
GATGTGTGGG ATGCACACTA TAAAATGTTT TGGTTTGATC ACCATGGCAA CAGTCCTGTG 
GCTAATATAC AGGAAGCAGA ACAGGCTGCT GAATTTAAGA AACGGTGGAC ACAGTTTGTT 
AATGTTACAT TTCATATGGA ATCCACAATC ACAAGAATTG CTTATGTATT TTATGGGCCA 
TATGTCAATG TTTCCAGCTG CAGATTTATT GATAGTTCCA GTTCTGGACT TCAGATTTCT 
TTACATGTCA ACAGTACTGA ACATAGTGTG TCTGTTGTAA CTGACTATCA AAACCTTAAA 
AGCAGATTCA GTTACCTGGG ATTTGGTGGT TTTGCCAGTG TGGCTAATCA AGGACAGATA 
ACCAGATTTG GTTTGGGTAC TCAAGAAATA GTAAACCCTG TAAGACATGA TAAAGTTAAT 
TTCCCCTTTG GGTTTAAATT TAATATAGCA GTTGGATTCA TTTTGTGTAT TAGTTTGGTT 
ATTTTAACTT TTCAATGGCG GTTTTACCTT TCCTTTAGAA AGCTAATGCG CTGTGTATTA 
ATACTTGTTA TTGCCTTGTG GTTTATTGAG CTTCTGGATG TATGGAGTAC ATGCACTCAG 
CCCATCTGTG CAAAATGGAC AAGGACTGAA GCTAAGGCAA ATGAGAAGGT CATGATTTCT 
GAAGGGCATC ATGTGGATCT TCCTAATGTT ATTATTACCT CACTCCCTGG TTCAGGAGCT 
GAAATTCTCA AACAGCTTTT TTTCAACAGC AGTGATTTTC TCTACATCAG AATTCCTACA 
GCCTACATGG ATATCCCTGA AACTGAATTT GAAATTGACT CATTTGTAGA TGCTTGTGAG 
TGGAAAGTAT CAGATATCCG CAGTGGGCAC TTTCATCTTC TTCGAGGGTG GCTGCAGTCT 
TTGGTCCAGG ATACAAAACT TCACTTGCAA AACATCCATC TACATGAAAC CAGTAGGAGT 
AAACTGGCCC AATATTTTAC AACTAATAAG GACAAAAAGC GAAAATTAAA AAGAAGGGAG 
TCTTTGCAAG ATCAAAGAAG TAGAATAAAA GGACCATTTG ATAGAGATGC TGAATATATT 
AGGGCTTTAA GAAGACACCT TGTTTATTAC CCAAGTGCAC GTCCTGTGCT CAGCTTAAGT 
AGTGGTAGCT GGACATTGAA GCTTCATTTT TTTCAGGAAG TTTTAGGAAC TTCAATGCGG 
GCATTGTACA TAGTAAGAGA CCCTCGAGCT TGGATCTATT CAGTGCTATA TGGTAGTAAA 
CCAAGTCTTT ATTCTTTGAA GAATGTACCA GAGCACTTAG CAAAATTGTT TAAAATAGAG 
GAAGGTAAAA GCAAATGTAA TTCGAATTCT GGCTATGCTT TTGAGTATGA ATCACTGAAG 
AAAGAATTAG AAATATCCCA ATCAAATGCT ATCTCCTTAT TATCTCATTT GTGGGTAGCA 
AACACTGCAG CAGCCTTGAG AATAAATACA GATTTGCTGC CTACCAATTA CCATCTGGTC 
AAGTTTGAAG ATATTGTTCA TTTTCCTCAG AAGACTACTG AAAGGATTTT TGCTTTCCTT 
GGCATTCCTT TGTCTCCTGC TAGTTTAAAC CAAATGCTAT TTGCCACTTC CACAAACCTT 
TTTTATCTTC CATATGAGGG GGAAATATCA CCATCTAATA CTAATATTTG GAAAACAAAC 
TTGCCTAGAG ATGAAATTAA ACTAATTGAA AACATTTGCT GGACACTGAT GGATCATCTA 
GGATATCCAA AGTTTATGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsel ( NM_001081316.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074785.1 Mus musculus dermatan sulfate epimerase-like (Dsel), mRNA.

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CloneID	OMu02102
Clone ID Related Accession (Same CDS sequence)	XM_017321184.1 , NM_001081316.1 , XM_030254948.1 , XM_017321183.1 , XM_017321183.2
Accession Version	NM_001081316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	dermatan-sulfate epimerase-like protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC125403.4. On Feb 6, 2007 this sequence version replaced XM_892762.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK033983.1, BQ952866.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164136, SAMN01164137 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTTTA TGTTTACAGA ACATTTACTA TTTTTAACAT TGATGATGTG TAGTTTTTCT 
ACTTGTGAAG AATCTGTGAG CAATTATTCT GAATGGGCAG TTTTCACAGA CGATATACAA 
TGGCTTAAGT CACAGAAAAT ACAAGATTTC AAACTCAACC GAAGACTTCA TCCAAATTTA 
TATTTTGATG CTGGAGATAT ACAAACATTG AAACAAAAGT CTCGTACAAG CCATTTGCAT 
ATTTTTAGAG CTATCAAAAG TGCAGTGACA ATTATGCTGT CCAATCCATC ATACTACCTA 
CCTCCACCCA AGCATGCTGA GTTTGCTGCC AAGTGGAATG AAATTTATGG TAATAATCTT 
CCTCCTTTAG CATTGTATTG TTTATTATGC CCAGAAGACA AGGTTGCCTT TGAATTTGTT 
ATGGAATACA TGGATCGGAT GGTTAGCTAC AAAGACTGGC TAGTTGAGAA TGCACCAGGG 
GATGAGGTTC CAGTTGGCCA TTCTTTAACA GGTTTTGCCA CTGCCTTTGA CTTTTTATAT 
AATCTATTAG GTAATCAGCG TAAACAAAAA TACCTAGAAA AAATTTGGAT TGTTACTGAG 
GAAATGTATG AATATTCCAA GATTCGATCA TGGGGCAAAC AACTTCTTCA TAACCATCAA 
GCTACAAATA TGATAGCTTT ACTCATAGGG GCCTTGGTTA CTGGAGTAGA TAAAGGATCT 
AAAGCAAACA TATGGAAACA AGTTGTTGTT GATGTGATGG AAAAGACTAT GTTTCTCTTG 
AAGCATATTG TAGATGGCTC ATTGGATGAA GGTGTGGCCT ATGGAAGCTA TACCTCAAAA 
TCAGTTACAC AGTATGTTTT TTTGGCACAA CGCCATTTTA ACATCAACAA CTTTGATAAT 
AACTGGCTAA AAATGCATTT TTGGTTTTAT TATGCTACAC TTTTGCCAGG CTATCAAAGA 
ACTGTAGGCA TAGCAGATTC CAATTATAAT TGGTTTTATG GTCCAGAGAG CCAGCTAGTT 
TTCTTGGATA AGTTCATTTT ACAGAATGGA GCTGGAAATT GGTTAGCTCA GCAAATTAGA 
AAGCATCGAC CTAAGGATGG ACCAATGGTT CCTTCCACTG CTCAGCGGTG GAGTACTCTT 
CATACTGAAT ACATCTGGTA TGATCCAACA CTCACCCCAC AGCCTCCTGT TGATTTTGGC 
ACTGCAAAAA TGCACACATT TCCTAACTGG GGTGTCGTGA CTTATGGGGG TGGGCTGCCA 
AACACCCAGA CCAATACCTT TGTGTCTTTT AAATCTGGGA AACTGGGAGG ACGAGCTGTG 
TATGACATAG TTCACTTTCA GCCATATTCC TGGATTGATG GATGGAGAAG CTTTAACCCA 
GGACATGAAC ATCCAGATCA AAATTCATTT ACTTTCGCTC CTAATGGGCA GGTATTCGTT 
TCTGAGGCTC TTTATGGACC AAAATTGAGC CACCTTAACA ACGTATTGGT GTTTGCCCCA 
TCACCATCAA GTCAATGTAA TCAGCCCTGG GAAGGTCAAC TGGGAGAATG TGCACAGTGG 
CTCAAGTGGA CTGGGGAAGA GGTTGGTGAT GCAGCTGGGG AAGTTATTAC TGCTGCTCAA 
CATGGTGATA GGATGTTTGT GAGTGGGGAA GCAGTGTCTG CTTATTCTTC TGCCATGAGA 
CTGAAAAGTG TCTATCGTGC TTTACTTCTT TTAAATTCAC AAACTCTGCT TGTTGTCGAT 
CATATTGAAA GGCAAGAAAC TTCCCCAATA AATTCTGTCA GTGCCTTCTT TCATAATTTG 
GATATTGATT TTAAATACAT CCCATACAAG TTTATGAATA GATATAATGG TGCCATGATG 
GATGTGTGGG ATGCACACTA TAAAATGTTT TGGTTTGATC ACCATGGCAA CAGTCCTGTG 
GCTAATATAC AGGAAGCAGA ACAGGCTGCT GAATTTAAGA AACGGTGGAC ACAGTTTGTT 
AATGTTACAT TTCATATGGA ATCCACAATC ACAAGAATTG CTTATGTATT TTATGGGCCA 
TATGTCAATG TTTCCAGCTG CAGATTTATT GATAGTTCCA GTTCTGGACT TCAGATTTCT 
TTACATGTCA ACAGTACTGA ACATAGTGTG TCTGTTGTAA CTGACTATCA AAACCTTAAA 
AGCAGATTCA GTTACCTGGG ATTTGGTGGT TTTGCCAGTG TGGCTAATCA AGGACAGATA 
ACCAGATTTG GTTTGGGTAC TCAAGAAATA GTAAACCCTG TAAGACATGA TAAAGTTAAT 
TTCCCCTTTG GGTTTAAATT TAATATAGCA GTTGGATTCA TTTTGTGTAT TAGTTTGGTT 
ATTTTAACTT TTCAATGGCG GTTTTACCTT TCCTTTAGAA AGCTAATGCG CTGTGTATTA 
ATACTTGTTA TTGCCTTGTG GTTTATTGAG CTTCTGGATG TATGGAGTAC ATGCACTCAG 
CCCATCTGTG CAAAATGGAC AAGGACTGAA GCTAAGGCAA ATGAGAAGGT CATGATTTCT 
GAAGGGCATC ATGTGGATCT TCCTAATGTT ATTATTACCT CACTCCCTGG TTCAGGAGCT 
GAAATTCTCA AACAGCTTTT TTTCAACAGC AGTGATTTTC TCTACATCAG AATTCCTACA 
GCCTACATGG ATATCCCTGA AACTGAATTT GAAATTGACT CATTTGTAGA TGCTTGTGAG 
TGGAAAGTAT CAGATATCCG CAGTGGGCAC TTTCATCTTC TTCGAGGGTG GCTGCAGTCT 
TTGGTCCAGG ATACAAAACT TCACTTGCAA AACATCCATC TACATGAAAC CAGTAGGAGT 
AAACTGGCCC AATATTTTAC AACTAATAAG GACAAAAAGC GAAAATTAAA AAGAAGGGAG 
TCTTTGCAAG ATCAAAGAAG TAGAATAAAA GGACCATTTG ATAGAGATGC TGAATATATT 
AGGGCTTTAA GAAGACACCT TGTTTATTAC CCAAGTGCAC GTCCTGTGCT CAGCTTAAGT 
AGTGGTAGCT GGACATTGAA GCTTCATTTT TTTCAGGAAG TTTTAGGAAC TTCAATGCGG 
GCATTGTACA TAGTAAGAGA CCCTCGAGCT TGGATCTATT CAGTGCTATA TGGTAGTAAA 
CCAAGTCTTT ATTCTTTGAA GAATGTACCA GAGCACTTAG CAAAATTGTT TAAAATAGAG 
GAAGGTAAAA GCAAATGTAA TTCGAATTCT GGCTATGCTT TTGAGTATGA ATCACTGAAG 
AAAGAATTAG AAATATCCCA ATCAAATGCT ATCTCCTTAT TATCTCATTT GTGGGTAGCA 
AACACTGCAG CAGCCTTGAG AATAAATACA GATTTGCTGC CTACCAATTA CCATCTGGTC 
AAGTTTGAAG ATATTGTTCA TTTTCCTCAG AAGACTACTG AAAGGATTTT TGCTTTCCTT 
GGCATTCCTT TGTCTCCTGC TAGTTTAAAC CAAATGCTAT TTGCCACTTC CACAAACCTT 
TTTTATCTTC CATATGAGGG GGAAATATCA CCATCTAATA CTAATATTTG GAAAACAAAC 
TTGCCTAGAG ATGAAATTAA ACTAATTGAA AACATTTGCT GGACACTGAT GGATCATCTA 
GGATATCCAA AGTTTATGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074785.1
CDS	1662..5285
Translation	MAFMFTEHLLFLTLMMCSFSTCEESVSNYSEWAVFTDDIQWLKSQKIQDFKLNRRLHPNLYFDAGDIQTLKQKSRTSHLHIFRAIKSAVTIMLSNPSYYLPPPKHAEFAAKWNEIYGNNLPPLALYCLLCPEDKVAFEFVMEYMDRMVSYKDWLVENAPGDEVPVGHSLTGFATAFDFLYNLLGNQRKQKYLEKIWIVTEEMYEYSKIRSWGKQLLHNHQATNMIALLIGALVTGVDKGSKANIWKQVVVDVMEKTMFLLKHIVDGSLDEGVAYGSYTSKSVTQYVFLAQRHFNINNFDNNWLKMHFWFYYATLLPGYQRTVGIADSNYNWFYGPESQLVFLDKFILQNGAGNWLAQQIRKHRPKDGPMVPSTAQRWSTLHTEYIWYDPTLTPQPPVDFGTAKMHTFPNWGVVTYGGGLPNTQTNTFVSFKSGKLGGRAVYDIVHFQPYSWIDGWRSFNPGHEHPDQNSFTFAPNGQVFVSEALYGPKLSHLNNVLVFAPSPSSQCNQPWEGQLGECAQWLKWTGEEVGDAAGEVITAAQHGDRMFVSGEAVSAYSSAMRLKSVYRALLLLNSQTLLVVDHIERQETSPINSVSAFFHNLDIDFKYIPYKFMNRYNGAMMDVWDAHYKMFWFDHHGNSPVANIQEAEQAAEFKKRWTQFVNVTFHMESTITRIAYVFYGPYVNVSSCRFIDSSSSGLQISLHVNSTEHSVSVVTDYQNLKSRFSYLGFGGFASVANQGQITRFGLGTQEIVNPVRHDKVNFPFGFKFNIAVGFILCISLVILTFQWRFYLSFRKLMRCVLILVIALWFIELLDVWSTCTQPICAKWTRTEAKANEKVMISEGHHVDLPNVIITSLPGSGAEILKQLFFNSSDFLYIRIPTAYMDIPETEFEIDSFVDACEWKVSDIRSGHFHLLRGWLQSLVQDTKLHLQNIHLHETSRSKLAQYFTTNKDKKRKLKRRESLQDQRSRIKGPFDRDAEYIRALRRHLVYYPSARPVLSLSSGSWTLKLHFFQEVLGTSMRALYIVRDPRAWIYSVLYGSKPSLYSLKNVPEHLAKLFKIEEGKSKCNSNSGYAFEYESLKKELEISQSNAISLLSHLWVANTAAALRINTDLLPTNYHLVKFEDIVHFPQKTTERIFAFLGIPLSPASLNQMLFATSTNLFYLPYEGEISPSNTNIWKTNLPRDEIKLIENICWTLMDHLGYPKFMD

Target ORF information:

RefSeq Version	NM_001081316.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dermatan sulfate epimerase-like (Dsel), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081316.1

ORF Insert Sequence:

ATGGCGTTTA TGTTTACAGA ACATTTACTA TTTTTAACAT TGATGATGTG TAGTTTTTCT 
ACTTGTGAAG AATCTGTGAG CAATTATTCT GAATGGGCAG TTTTCACAGA CGATATACAA 
TGGCTTAAGT CACAGAAAAT ACAAGATTTC AAACTCAACC GAAGACTTCA TCCAAATTTA 
TATTTTGATG CTGGAGATAT ACAAACATTG AAACAAAAGT CTCGTACAAG CCATTTGCAT 
ATTTTTAGAG CTATCAAAAG TGCAGTGACA ATTATGCTGT CCAATCCATC ATACTACCTA 
CCTCCACCCA AGCATGCTGA GTTTGCTGCC AAGTGGAATG AAATTTATGG TAATAATCTT 
CCTCCTTTAG CATTGTATTG TTTATTATGC CCAGAAGACA AGGTTGCCTT TGAATTTGTT 
ATGGAATACA TGGATCGGAT GGTTAGCTAC AAAGACTGGC TAGTTGAGAA TGCACCAGGG 
GATGAGGTTC CAGTTGGCCA TTCTTTAACA GGTTTTGCCA CTGCCTTTGA CTTTTTATAT 
AATCTATTAG GTAATCAGCG TAAACAAAAA TACCTAGAAA AAATTTGGAT TGTTACTGAG 
GAAATGTATG AATATTCCAA GATTCGATCA TGGGGCAAAC AACTTCTTCA TAACCATCAA 
GCTACAAATA TGATAGCTTT ACTCATAGGG GCCTTGGTTA CTGGAGTAGA TAAAGGATCT 
AAAGCAAACA TATGGAAACA AGTTGTTGTT GATGTGATGG AAAAGACTAT GTTTCTCTTG 
AAGCATATTG TAGATGGCTC ATTGGATGAA GGTGTGGCCT ATGGAAGCTA TACCTCAAAA 
TCAGTTACAC AGTATGTTTT TTTGGCACAA CGCCATTTTA ACATCAACAA CTTTGATAAT 
AACTGGCTAA AAATGCATTT TTGGTTTTAT TATGCTACAC TTTTGCCAGG CTATCAAAGA 
ACTGTAGGCA TAGCAGATTC CAATTATAAT TGGTTTTATG GTCCAGAGAG CCAGCTAGTT 
TTCTTGGATA AGTTCATTTT ACAGAATGGA GCTGGAAATT GGTTAGCTCA GCAAATTAGA 
AAGCATCGAC CTAAGGATGG ACCAATGGTT CCTTCCACTG CTCAGCGGTG GAGTACTCTT 
CATACTGAAT ACATCTGGTA TGATCCAACA CTCACCCCAC AGCCTCCTGT TGATTTTGGC 
ACTGCAAAAA TGCACACATT TCCTAACTGG GGTGTCGTGA CTTATGGGGG TGGGCTGCCA 
AACACCCAGA CCAATACCTT TGTGTCTTTT AAATCTGGGA AACTGGGAGG ACGAGCTGTG 
TATGACATAG TTCACTTTCA GCCATATTCC TGGATTGATG GATGGAGAAG CTTTAACCCA 
GGACATGAAC ATCCAGATCA AAATTCATTT ACTTTCGCTC CTAATGGGCA GGTATTCGTT 
TCTGAGGCTC TTTATGGACC AAAATTGAGC CACCTTAACA ACGTATTGGT GTTTGCCCCA 
TCACCATCAA GTCAATGTAA TCAGCCCTGG GAAGGTCAAC TGGGAGAATG TGCACAGTGG 
CTCAAGTGGA CTGGGGAAGA GGTTGGTGAT GCAGCTGGGG AAGTTATTAC TGCTGCTCAA 
CATGGTGATA GGATGTTTGT GAGTGGGGAA GCAGTGTCTG CTTATTCTTC TGCCATGAGA 
CTGAAAAGTG TCTATCGTGC TTTACTTCTT TTAAATTCAC AAACTCTGCT TGTTGTCGAT 
CATATTGAAA GGCAAGAAAC TTCCCCAATA AATTCTGTCA GTGCCTTCTT TCATAATTTG 
GATATTGATT TTAAATACAT CCCATACAAG TTTATGAATA GATATAATGG TGCCATGATG 
GATGTGTGGG ATGCACACTA TAAAATGTTT TGGTTTGATC ACCATGGCAA CAGTCCTGTG 
GCTAATATAC AGGAAGCAGA ACAGGCTGCT GAATTTAAGA AACGGTGGAC ACAGTTTGTT 
AATGTTACAT TTCATATGGA ATCCACAATC ACAAGAATTG CTTATGTATT TTATGGGCCA 
TATGTCAATG TTTCCAGCTG CAGATTTATT GATAGTTCCA GTTCTGGACT TCAGATTTCT 
TTACATGTCA ACAGTACTGA ACATAGTGTG TCTGTTGTAA CTGACTATCA AAACCTTAAA 
AGCAGATTCA GTTACCTGGG ATTTGGTGGT TTTGCCAGTG TGGCTAATCA AGGACAGATA 
ACCAGATTTG GTTTGGGTAC TCAAGAAATA GTAAACCCTG TAAGACATGA TAAAGTTAAT 
TTCCCCTTTG GGTTTAAATT TAATATAGCA GTTGGATTCA TTTTGTGTAT TAGTTTGGTT 
ATTTTAACTT TTCAATGGCG GTTTTACCTT TCCTTTAGAA AGCTAATGCG CTGTGTATTA 
ATACTTGTTA TTGCCTTGTG GTTTATTGAG CTTCTGGATG TATGGAGTAC ATGCACTCAG 
CCCATCTGTG CAAAATGGAC AAGGACTGAA GCTAAGGCAA ATGAGAAGGT CATGATTTCT 
GAAGGGCATC ATGTGGATCT TCCTAATGTT ATTATTACCT CACTCCCTGG TTCAGGAGCT 
GAAATTCTCA AACAGCTTTT TTTCAACAGC AGTGATTTTC TCTACATCAG AATTCCTACA 
GCCTACATGG ATATCCCTGA AACTGAATTT GAAATTGACT CATTTGTAGA TGCTTGTGAG 
TGGAAAGTAT CAGATATCCG CAGTGGGCAC TTTCATCTTC TTCGAGGGTG GCTGCAGTCT 
TTGGTCCAGG ATACAAAACT TCACTTGCAA AACATCCATC TACATGAAAC CAGTAGGAGT 
AAACTGGCCC AATATTTTAC AACTAATAAG GACAAAAAGC GAAAATTAAA AAGAAGGGAG 
TCTTTGCAAG ATCAAAGAAG TAGAATAAAA GGACCATTTG ATAGAGATGC TGAATATATT 
AGGGCTTTAA GAAGACACCT TGTTTATTAC CCAAGTGCAC GTCCTGTGCT CAGCTTAAGT 
AGTGGTAGCT GGACATTGAA GCTTCATTTT TTTCAGGAAG TTTTAGGAAC TTCAATGCGG 
GCATTGTACA TAGTAAGAGA CCCTCGAGCT TGGATCTATT CAGTGCTATA TGGTAGTAAA 
CCAAGTCTTT ATTCTTTGAA GAATGTACCA GAGCACTTAG CAAAATTGTT TAAAATAGAG 
GAAGGTAAAA GCAAATGTAA TTCGAATTCT GGCTATGCTT TTGAGTATGA ATCACTGAAG 
AAAGAATTAG AAATATCCCA ATCAAATGCT ATCTCCTTAT TATCTCATTT GTGGGTAGCA 
AACACTGCAG CAGCCTTGAG AATAAATACA GATTTGCTGC CTACCAATTA CCATCTGGTC 
AAGTTTGAAG ATATTGTTCA TTTTCCTCAG AAGACTACTG AAAGGATTTT TGCTTTCCTT 
GGCATTCCTT TGTCTCCTGC TAGTTTAAAC CAAATGCTAT TTGCCACTTC CACAAACCTT 
TTTTATCTTC CATATGAGGG GGAAATATCA CCATCTAATA CTAATATTTG GAAAACAAAC 
TTGCCTAGAG ATGAAATTAA ACTAATTGAA AACATTTGCT GGACACTGAT GGATCATCTA 
GGATATCCAA AGTTTATGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsel ( XM_017321184.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176673.1 Mus musculus dermatan sulfate epimerase-like (Dsel), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu02102
Clone ID Related Accession (Same CDS sequence)	XM_017321184.1 , NM_001081316.1 , XM_030254948.1 , XM_017321183.1 , XM_017321183.2
Accession Version	XM_017321184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	dermatan-sulfate epimerase-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTTA TGTTTACAGA ACATTTACTA TTTTTAACAT TGATGATGTG TAGTTTTTCT 
ACTTGTGAAG AATCTGTGAG CAATTATTCT GAATGGGCAG TTTTCACAGA CGATATACAA 
TGGCTTAAGT CACAGAAAAT ACAAGATTTC AAACTCAACC GAAGACTTCA TCCAAATTTA 
TATTTTGATG CTGGAGATAT ACAAACATTG AAACAAAAGT CTCGTACAAG CCATTTGCAT 
ATTTTTAGAG CTATCAAAAG TGCAGTGACA ATTATGCTGT CCAATCCATC ATACTACCTA 
CCTCCACCCA AGCATGCTGA GTTTGCTGCC AAGTGGAATG AAATTTATGG TAATAATCTT 
CCTCCTTTAG CATTGTATTG TTTATTATGC CCAGAAGACA AGGTTGCCTT TGAATTTGTT 
ATGGAATACA TGGATCGGAT GGTTAGCTAC AAAGACTGGC TAGTTGAGAA TGCACCAGGG 
GATGAGGTTC CAGTTGGCCA TTCTTTAACA GGTTTTGCCA CTGCCTTTGA CTTTTTATAT 
AATCTATTAG GTAATCAGCG TAAACAAAAA TACCTAGAAA AAATTTGGAT TGTTACTGAG 
GAAATGTATG AATATTCCAA GATTCGATCA TGGGGCAAAC AACTTCTTCA TAACCATCAA 
GCTACAAATA TGATAGCTTT ACTCATAGGG GCCTTGGTTA CTGGAGTAGA TAAAGGATCT 
AAAGCAAACA TATGGAAACA AGTTGTTGTT GATGTGATGG AAAAGACTAT GTTTCTCTTG 
AAGCATATTG TAGATGGCTC ATTGGATGAA GGTGTGGCCT ATGGAAGCTA TACCTCAAAA 
TCAGTTACAC AGTATGTTTT TTTGGCACAA CGCCATTTTA ACATCAACAA CTTTGATAAT 
AACTGGCTAA AAATGCATTT TTGGTTTTAT TATGCTACAC TTTTGCCAGG CTATCAAAGA 
ACTGTAGGCA TAGCAGATTC CAATTATAAT TGGTTTTATG GTCCAGAGAG CCAGCTAGTT 
TTCTTGGATA AGTTCATTTT ACAGAATGGA GCTGGAAATT GGTTAGCTCA GCAAATTAGA 
AAGCATCGAC CTAAGGATGG ACCAATGGTT CCTTCCACTG CTCAGCGGTG GAGTACTCTT 
CATACTGAAT ACATCTGGTA TGATCCAACA CTCACCCCAC AGCCTCCTGT TGATTTTGGC 
ACTGCAAAAA TGCACACATT TCCTAACTGG GGTGTCGTGA CTTATGGGGG TGGGCTGCCA 
AACACCCAGA CCAATACCTT TGTGTCTTTT AAATCTGGGA AACTGGGAGG ACGAGCTGTG 
TATGACATAG TTCACTTTCA GCCATATTCC TGGATTGATG GATGGAGAAG CTTTAACCCA 
GGACATGAAC ATCCAGATCA AAATTCATTT ACTTTCGCTC CTAATGGGCA GGTATTCGTT 
TCTGAGGCTC TTTATGGACC AAAATTGAGC CACCTTAACA ACGTATTGGT GTTTGCCCCA 
TCACCATCAA GTCAATGTAA TCAGCCCTGG GAAGGTCAAC TGGGAGAATG TGCACAGTGG 
CTCAAGTGGA CTGGGGAAGA GGTTGGTGAT GCAGCTGGGG AAGTTATTAC TGCTGCTCAA 
CATGGTGATA GGATGTTTGT GAGTGGGGAA GCAGTGTCTG CTTATTCTTC TGCCATGAGA 
CTGAAAAGTG TCTATCGTGC TTTACTTCTT TTAAATTCAC AAACTCTGCT TGTTGTCGAT 
CATATTGAAA GGCAAGAAAC TTCCCCAATA AATTCTGTCA GTGCCTTCTT TCATAATTTG 
GATATTGATT TTAAATACAT CCCATACAAG TTTATGAATA GATATAATGG TGCCATGATG 
GATGTGTGGG ATGCACACTA TAAAATGTTT TGGTTTGATC ACCATGGCAA CAGTCCTGTG 
GCTAATATAC AGGAAGCAGA ACAGGCTGCT GAATTTAAGA AACGGTGGAC ACAGTTTGTT 
AATGTTACAT TTCATATGGA ATCCACAATC ACAAGAATTG CTTATGTATT TTATGGGCCA 
TATGTCAATG TTTCCAGCTG CAGATTTATT GATAGTTCCA GTTCTGGACT TCAGATTTCT 
TTACATGTCA ACAGTACTGA ACATAGTGTG TCTGTTGTAA CTGACTATCA AAACCTTAAA 
AGCAGATTCA GTTACCTGGG ATTTGGTGGT TTTGCCAGTG TGGCTAATCA AGGACAGATA 
ACCAGATTTG GTTTGGGTAC TCAAGAAATA GTAAACCCTG TAAGACATGA TAAAGTTAAT 
TTCCCCTTTG GGTTTAAATT TAATATAGCA GTTGGATTCA TTTTGTGTAT TAGTTTGGTT 
ATTTTAACTT TTCAATGGCG GTTTTACCTT TCCTTTAGAA AGCTAATGCG CTGTGTATTA 
ATACTTGTTA TTGCCTTGTG GTTTATTGAG CTTCTGGATG TATGGAGTAC ATGCACTCAG 
CCCATCTGTG CAAAATGGAC AAGGACTGAA GCTAAGGCAA ATGAGAAGGT CATGATTTCT 
GAAGGGCATC ATGTGGATCT TCCTAATGTT ATTATTACCT CACTCCCTGG TTCAGGAGCT 
GAAATTCTCA AACAGCTTTT TTTCAACAGC AGTGATTTTC TCTACATCAG AATTCCTACA 
GCCTACATGG ATATCCCTGA AACTGAATTT GAAATTGACT CATTTGTAGA TGCTTGTGAG 
TGGAAAGTAT CAGATATCCG CAGTGGGCAC TTTCATCTTC TTCGAGGGTG GCTGCAGTCT 
TTGGTCCAGG ATACAAAACT TCACTTGCAA AACATCCATC TACATGAAAC CAGTAGGAGT 
AAACTGGCCC AATATTTTAC AACTAATAAG GACAAAAAGC GAAAATTAAA AAGAAGGGAG 
TCTTTGCAAG ATCAAAGAAG TAGAATAAAA GGACCATTTG ATAGAGATGC TGAATATATT 
AGGGCTTTAA GAAGACACCT TGTTTATTAC CCAAGTGCAC GTCCTGTGCT CAGCTTAAGT 
AGTGGTAGCT GGACATTGAA GCTTCATTTT TTTCAGGAAG TTTTAGGAAC TTCAATGCGG 
GCATTGTACA TAGTAAGAGA CCCTCGAGCT TGGATCTATT CAGTGCTATA TGGTAGTAAA 
CCAAGTCTTT ATTCTTTGAA GAATGTACCA GAGCACTTAG CAAAATTGTT TAAAATAGAG 
GAAGGTAAAA GCAAATGTAA TTCGAATTCT GGCTATGCTT TTGAGTATGA ATCACTGAAG 
AAAGAATTAG AAATATCCCA ATCAAATGCT ATCTCCTTAT TATCTCATTT GTGGGTAGCA 
AACACTGCAG CAGCCTTGAG AATAAATACA GATTTGCTGC CTACCAATTA CCATCTGGTC 
AAGTTTGAAG ATATTGTTCA TTTTCCTCAG AAGACTACTG AAAGGATTTT TGCTTTCCTT 
GGCATTCCTT TGTCTCCTGC TAGTTTAAAC CAAATGCTAT TTGCCACTTC CACAAACCTT 
TTTTATCTTC CATATGAGGG GGAAATATCA CCATCTAATA CTAATATTTG GAAAACAAAC 
TTGCCTAGAG ATGAAATTAA ACTAATTGAA AACATTTGCT GGACACTGAT GGATCATCTA 
GGATATCCAA AGTTTATGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176673.1
CDS	1454..5077
Translation	MAFMFTEHLLFLTLMMCSFSTCEESVSNYSEWAVFTDDIQWLKSQKIQDFKLNRRLHPNLYFDAGDIQTLKQKSRTSHLHIFRAIKSAVTIMLSNPSYYLPPPKHAEFAAKWNEIYGNNLPPLALYCLLCPEDKVAFEFVMEYMDRMVSYKDWLVENAPGDEVPVGHSLTGFATAFDFLYNLLGNQRKQKYLEKIWIVTEEMYEYSKIRSWGKQLLHNHQATNMIALLIGALVTGVDKGSKANIWKQVVVDVMEKTMFLLKHIVDGSLDEGVAYGSYTSKSVTQYVFLAQRHFNINNFDNNWLKMHFWFYYATLLPGYQRTVGIADSNYNWFYGPESQLVFLDKFILQNGAGNWLAQQIRKHRPKDGPMVPSTAQRWSTLHTEYIWYDPTLTPQPPVDFGTAKMHTFPNWGVVTYGGGLPNTQTNTFVSFKSGKLGGRAVYDIVHFQPYSWIDGWRSFNPGHEHPDQNSFTFAPNGQVFVSEALYGPKLSHLNNVLVFAPSPSSQCNQPWEGQLGECAQWLKWTGEEVGDAAGEVITAAQHGDRMFVSGEAVSAYSSAMRLKSVYRALLLLNSQTLLVVDHIERQETSPINSVSAFFHNLDIDFKYIPYKFMNRYNGAMMDVWDAHYKMFWFDHHGNSPVANIQEAEQAAEFKKRWTQFVNVTFHMESTITRIAYVFYGPYVNVSSCRFIDSSSSGLQISLHVNSTEHSVSVVTDYQNLKSRFSYLGFGGFASVANQGQITRFGLGTQEIVNPVRHDKVNFPFGFKFNIAVGFILCISLVILTFQWRFYLSFRKLMRCVLILVIALWFIELLDVWSTCTQPICAKWTRTEAKANEKVMISEGHHVDLPNVIITSLPGSGAEILKQLFFNSSDFLYIRIPTAYMDIPETEFEIDSFVDACEWKVSDIRSGHFHLLRGWLQSLVQDTKLHLQNIHLHETSRSKLAQYFTTNKDKKRKLKRRESLQDQRSRIKGPFDRDAEYIRALRRHLVYYPSARPVLSLSSGSWTLKLHFFQEVLGTSMRALYIVRDPRAWIYSVLYGSKPSLYSLKNVPEHLAKLFKIEEGKSKCNSNSGYAFEYESLKKELEISQSNAISLLSHLWVANTAAALRINTDLLPTNYHLVKFEDIVHFPQKTTERIFAFLGIPLSPASLNQMLFATSTNLFYLPYEGEISPSNTNIWKTNLPRDEIKLIENICWTLMDHLGYPKFMD

Target ORF information:

RefSeq Version	XM_017321184.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dermatan sulfate epimerase-like (Dsel), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321184.1

ORF Insert Sequence:

ATGGCGTTTA TGTTTACAGA ACATTTACTA TTTTTAACAT TGATGATGTG TAGTTTTTCT 
ACTTGTGAAG AATCTGTGAG CAATTATTCT GAATGGGCAG TTTTCACAGA CGATATACAA 
TGGCTTAAGT CACAGAAAAT ACAAGATTTC AAACTCAACC GAAGACTTCA TCCAAATTTA 
TATTTTGATG CTGGAGATAT ACAAACATTG AAACAAAAGT CTCGTACAAG CCATTTGCAT 
ATTTTTAGAG CTATCAAAAG TGCAGTGACA ATTATGCTGT CCAATCCATC ATACTACCTA 
CCTCCACCCA AGCATGCTGA GTTTGCTGCC AAGTGGAATG AAATTTATGG TAATAATCTT 
CCTCCTTTAG CATTGTATTG TTTATTATGC CCAGAAGACA AGGTTGCCTT TGAATTTGTT 
ATGGAATACA TGGATCGGAT GGTTAGCTAC AAAGACTGGC TAGTTGAGAA TGCACCAGGG 
GATGAGGTTC CAGTTGGCCA TTCTTTAACA GGTTTTGCCA CTGCCTTTGA CTTTTTATAT 
AATCTATTAG GTAATCAGCG TAAACAAAAA TACCTAGAAA AAATTTGGAT TGTTACTGAG 
GAAATGTATG AATATTCCAA GATTCGATCA TGGGGCAAAC AACTTCTTCA TAACCATCAA 
GCTACAAATA TGATAGCTTT ACTCATAGGG GCCTTGGTTA CTGGAGTAGA TAAAGGATCT 
AAAGCAAACA TATGGAAACA AGTTGTTGTT GATGTGATGG AAAAGACTAT GTTTCTCTTG 
AAGCATATTG TAGATGGCTC ATTGGATGAA GGTGTGGCCT ATGGAAGCTA TACCTCAAAA 
TCAGTTACAC AGTATGTTTT TTTGGCACAA CGCCATTTTA ACATCAACAA CTTTGATAAT 
AACTGGCTAA AAATGCATTT TTGGTTTTAT TATGCTACAC TTTTGCCAGG CTATCAAAGA 
ACTGTAGGCA TAGCAGATTC CAATTATAAT TGGTTTTATG GTCCAGAGAG CCAGCTAGTT 
TTCTTGGATA AGTTCATTTT ACAGAATGGA GCTGGAAATT GGTTAGCTCA GCAAATTAGA 
AAGCATCGAC CTAAGGATGG ACCAATGGTT CCTTCCACTG CTCAGCGGTG GAGTACTCTT 
CATACTGAAT ACATCTGGTA TGATCCAACA CTCACCCCAC AGCCTCCTGT TGATTTTGGC 
ACTGCAAAAA TGCACACATT TCCTAACTGG GGTGTCGTGA CTTATGGGGG TGGGCTGCCA 
AACACCCAGA CCAATACCTT TGTGTCTTTT AAATCTGGGA AACTGGGAGG ACGAGCTGTG 
TATGACATAG TTCACTTTCA GCCATATTCC TGGATTGATG GATGGAGAAG CTTTAACCCA 
GGACATGAAC ATCCAGATCA AAATTCATTT ACTTTCGCTC CTAATGGGCA GGTATTCGTT 
TCTGAGGCTC TTTATGGACC AAAATTGAGC CACCTTAACA ACGTATTGGT GTTTGCCCCA 
TCACCATCAA GTCAATGTAA TCAGCCCTGG GAAGGTCAAC TGGGAGAATG TGCACAGTGG 
CTCAAGTGGA CTGGGGAAGA GGTTGGTGAT GCAGCTGGGG AAGTTATTAC TGCTGCTCAA 
CATGGTGATA GGATGTTTGT GAGTGGGGAA GCAGTGTCTG CTTATTCTTC TGCCATGAGA 
CTGAAAAGTG TCTATCGTGC TTTACTTCTT TTAAATTCAC AAACTCTGCT TGTTGTCGAT 
CATATTGAAA GGCAAGAAAC TTCCCCAATA AATTCTGTCA GTGCCTTCTT TCATAATTTG 
GATATTGATT TTAAATACAT CCCATACAAG TTTATGAATA GATATAATGG TGCCATGATG 
GATGTGTGGG ATGCACACTA TAAAATGTTT TGGTTTGATC ACCATGGCAA CAGTCCTGTG 
GCTAATATAC AGGAAGCAGA ACAGGCTGCT GAATTTAAGA AACGGTGGAC ACAGTTTGTT 
AATGTTACAT TTCATATGGA ATCCACAATC ACAAGAATTG CTTATGTATT TTATGGGCCA 
TATGTCAATG TTTCCAGCTG CAGATTTATT GATAGTTCCA GTTCTGGACT TCAGATTTCT 
TTACATGTCA ACAGTACTGA ACATAGTGTG TCTGTTGTAA CTGACTATCA AAACCTTAAA 
AGCAGATTCA GTTACCTGGG ATTTGGTGGT TTTGCCAGTG TGGCTAATCA AGGACAGATA 
ACCAGATTTG GTTTGGGTAC TCAAGAAATA GTAAACCCTG TAAGACATGA TAAAGTTAAT 
TTCCCCTTTG GGTTTAAATT TAATATAGCA GTTGGATTCA TTTTGTGTAT TAGTTTGGTT 
ATTTTAACTT TTCAATGGCG GTTTTACCTT TCCTTTAGAA AGCTAATGCG CTGTGTATTA 
ATACTTGTTA TTGCCTTGTG GTTTATTGAG CTTCTGGATG TATGGAGTAC ATGCACTCAG 
CCCATCTGTG CAAAATGGAC AAGGACTGAA GCTAAGGCAA ATGAGAAGGT CATGATTTCT 
GAAGGGCATC ATGTGGATCT TCCTAATGTT ATTATTACCT CACTCCCTGG TTCAGGAGCT 
GAAATTCTCA AACAGCTTTT TTTCAACAGC AGTGATTTTC TCTACATCAG AATTCCTACA 
GCCTACATGG ATATCCCTGA AACTGAATTT GAAATTGACT CATTTGTAGA TGCTTGTGAG 
TGGAAAGTAT CAGATATCCG CAGTGGGCAC TTTCATCTTC TTCGAGGGTG GCTGCAGTCT 
TTGGTCCAGG ATACAAAACT TCACTTGCAA AACATCCATC TACATGAAAC CAGTAGGAGT 
AAACTGGCCC AATATTTTAC AACTAATAAG GACAAAAAGC GAAAATTAAA AAGAAGGGAG 
TCTTTGCAAG ATCAAAGAAG TAGAATAAAA GGACCATTTG ATAGAGATGC TGAATATATT 
AGGGCTTTAA GAAGACACCT TGTTTATTAC CCAAGTGCAC GTCCTGTGCT CAGCTTAAGT 
AGTGGTAGCT GGACATTGAA GCTTCATTTT TTTCAGGAAG TTTTAGGAAC TTCAATGCGG 
GCATTGTACA TAGTAAGAGA CCCTCGAGCT TGGATCTATT CAGTGCTATA TGGTAGTAAA 
CCAAGTCTTT ATTCTTTGAA GAATGTACCA GAGCACTTAG CAAAATTGTT TAAAATAGAG 
GAAGGTAAAA GCAAATGTAA TTCGAATTCT GGCTATGCTT TTGAGTATGA ATCACTGAAG 
AAAGAATTAG AAATATCCCA ATCAAATGCT ATCTCCTTAT TATCTCATTT GTGGGTAGCA 
AACACTGCAG CAGCCTTGAG AATAAATACA GATTTGCTGC CTACCAATTA CCATCTGGTC 
AAGTTTGAAG ATATTGTTCA TTTTCCTCAG AAGACTACTG AAAGGATTTT TGCTTTCCTT 
GGCATTCCTT TGTCTCCTGC TAGTTTAAAC CAAATGCTAT TTGCCACTTC CACAAACCTT 
TTTTATCTTC CATATGAGGG GGAAATATCA CCATCTAATA CTAATATTTG GAAAACAAAC 
TTGCCTAGAG ATGAAATTAA ACTAATTGAA AACATTTGCT GGACACTGAT GGATCATCTA 
GGATATCCAA AGTTTATGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsel ( XM_017321183.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176672.1 Mus musculus dermatan sulfate epimerase-like (Dsel), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu02102
Clone ID Related Accession (Same CDS sequence)	XM_017321184.1 , NM_001081316.1 , XM_030254948.1 , XM_017321183.1 , XM_017321183.2
Accession Version	XM_017321183.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	dermatan-sulfate epimerase-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTTA TGTTTACAGA ACATTTACTA TTTTTAACAT TGATGATGTG TAGTTTTTCT 
ACTTGTGAAG AATCTGTGAG CAATTATTCT GAATGGGCAG TTTTCACAGA CGATATACAA 
TGGCTTAAGT CACAGAAAAT ACAAGATTTC AAACTCAACC GAAGACTTCA TCCAAATTTA 
TATTTTGATG CTGGAGATAT ACAAACATTG AAACAAAAGT CTCGTACAAG CCATTTGCAT 
ATTTTTAGAG CTATCAAAAG TGCAGTGACA ATTATGCTGT CCAATCCATC ATACTACCTA 
CCTCCACCCA AGCATGCTGA GTTTGCTGCC AAGTGGAATG AAATTTATGG TAATAATCTT 
CCTCCTTTAG CATTGTATTG TTTATTATGC CCAGAAGACA AGGTTGCCTT TGAATTTGTT 
ATGGAATACA TGGATCGGAT GGTTAGCTAC AAAGACTGGC TAGTTGAGAA TGCACCAGGG 
GATGAGGTTC CAGTTGGCCA TTCTTTAACA GGTTTTGCCA CTGCCTTTGA CTTTTTATAT 
AATCTATTAG GTAATCAGCG TAAACAAAAA TACCTAGAAA AAATTTGGAT TGTTACTGAG 
GAAATGTATG AATATTCCAA GATTCGATCA TGGGGCAAAC AACTTCTTCA TAACCATCAA 
GCTACAAATA TGATAGCTTT ACTCATAGGG GCCTTGGTTA CTGGAGTAGA TAAAGGATCT 
AAAGCAAACA TATGGAAACA AGTTGTTGTT GATGTGATGG AAAAGACTAT GTTTCTCTTG 
AAGCATATTG TAGATGGCTC ATTGGATGAA GGTGTGGCCT ATGGAAGCTA TACCTCAAAA 
TCAGTTACAC AGTATGTTTT TTTGGCACAA CGCCATTTTA ACATCAACAA CTTTGATAAT 
AACTGGCTAA AAATGCATTT TTGGTTTTAT TATGCTACAC TTTTGCCAGG CTATCAAAGA 
ACTGTAGGCA TAGCAGATTC CAATTATAAT TGGTTTTATG GTCCAGAGAG CCAGCTAGTT 
TTCTTGGATA AGTTCATTTT ACAGAATGGA GCTGGAAATT GGTTAGCTCA GCAAATTAGA 
AAGCATCGAC CTAAGGATGG ACCAATGGTT CCTTCCACTG CTCAGCGGTG GAGTACTCTT 
CATACTGAAT ACATCTGGTA TGATCCAACA CTCACCCCAC AGCCTCCTGT TGATTTTGGC 
ACTGCAAAAA TGCACACATT TCCTAACTGG GGTGTCGTGA CTTATGGGGG TGGGCTGCCA 
AACACCCAGA CCAATACCTT TGTGTCTTTT AAATCTGGGA AACTGGGAGG ACGAGCTGTG 
TATGACATAG TTCACTTTCA GCCATATTCC TGGATTGATG GATGGAGAAG CTTTAACCCA 
GGACATGAAC ATCCAGATCA AAATTCATTT ACTTTCGCTC CTAATGGGCA GGTATTCGTT 
TCTGAGGCTC TTTATGGACC AAAATTGAGC CACCTTAACA ACGTATTGGT GTTTGCCCCA 
TCACCATCAA GTCAATGTAA TCAGCCCTGG GAAGGTCAAC TGGGAGAATG TGCACAGTGG 
CTCAAGTGGA CTGGGGAAGA GGTTGGTGAT GCAGCTGGGG AAGTTATTAC TGCTGCTCAA 
CATGGTGATA GGATGTTTGT GAGTGGGGAA GCAGTGTCTG CTTATTCTTC TGCCATGAGA 
CTGAAAAGTG TCTATCGTGC TTTACTTCTT TTAAATTCAC AAACTCTGCT TGTTGTCGAT 
CATATTGAAA GGCAAGAAAC TTCCCCAATA AATTCTGTCA GTGCCTTCTT TCATAATTTG 
GATATTGATT TTAAATACAT CCCATACAAG TTTATGAATA GATATAATGG TGCCATGATG 
GATGTGTGGG ATGCACACTA TAAAATGTTT TGGTTTGATC ACCATGGCAA CAGTCCTGTG 
GCTAATATAC AGGAAGCAGA ACAGGCTGCT GAATTTAAGA AACGGTGGAC ACAGTTTGTT 
AATGTTACAT TTCATATGGA ATCCACAATC ACAAGAATTG CTTATGTATT TTATGGGCCA 
TATGTCAATG TTTCCAGCTG CAGATTTATT GATAGTTCCA GTTCTGGACT TCAGATTTCT 
TTACATGTCA ACAGTACTGA ACATAGTGTG TCTGTTGTAA CTGACTATCA AAACCTTAAA 
AGCAGATTCA GTTACCTGGG ATTTGGTGGT TTTGCCAGTG TGGCTAATCA AGGACAGATA 
ACCAGATTTG GTTTGGGTAC TCAAGAAATA GTAAACCCTG TAAGACATGA TAAAGTTAAT 
TTCCCCTTTG GGTTTAAATT TAATATAGCA GTTGGATTCA TTTTGTGTAT TAGTTTGGTT 
ATTTTAACTT TTCAATGGCG GTTTTACCTT TCCTTTAGAA AGCTAATGCG CTGTGTATTA 
ATACTTGTTA TTGCCTTGTG GTTTATTGAG CTTCTGGATG TATGGAGTAC ATGCACTCAG 
CCCATCTGTG CAAAATGGAC AAGGACTGAA GCTAAGGCAA ATGAGAAGGT CATGATTTCT 
GAAGGGCATC ATGTGGATCT TCCTAATGTT ATTATTACCT CACTCCCTGG TTCAGGAGCT 
GAAATTCTCA AACAGCTTTT TTTCAACAGC AGTGATTTTC TCTACATCAG AATTCCTACA 
GCCTACATGG ATATCCCTGA AACTGAATTT GAAATTGACT CATTTGTAGA TGCTTGTGAG 
TGGAAAGTAT CAGATATCCG CAGTGGGCAC TTTCATCTTC TTCGAGGGTG GCTGCAGTCT 
TTGGTCCAGG ATACAAAACT TCACTTGCAA AACATCCATC TACATGAAAC CAGTAGGAGT 
AAACTGGCCC AATATTTTAC AACTAATAAG GACAAAAAGC GAAAATTAAA AAGAAGGGAG 
TCTTTGCAAG ATCAAAGAAG TAGAATAAAA GGACCATTTG ATAGAGATGC TGAATATATT 
AGGGCTTTAA GAAGACACCT TGTTTATTAC CCAAGTGCAC GTCCTGTGCT CAGCTTAAGT 
AGTGGTAGCT GGACATTGAA GCTTCATTTT TTTCAGGAAG TTTTAGGAAC TTCAATGCGG 
GCATTGTACA TAGTAAGAGA CCCTCGAGCT TGGATCTATT CAGTGCTATA TGGTAGTAAA 
CCAAGTCTTT ATTCTTTGAA GAATGTACCA GAGCACTTAG CAAAATTGTT TAAAATAGAG 
GAAGGTAAAA GCAAATGTAA TTCGAATTCT GGCTATGCTT TTGAGTATGA ATCACTGAAG 
AAAGAATTAG AAATATCCCA ATCAAATGCT ATCTCCTTAT TATCTCATTT GTGGGTAGCA 
AACACTGCAG CAGCCTTGAG AATAAATACA GATTTGCTGC CTACCAATTA CCATCTGGTC 
AAGTTTGAAG ATATTGTTCA TTTTCCTCAG AAGACTACTG AAAGGATTTT TGCTTTCCTT 
GGCATTCCTT TGTCTCCTGC TAGTTTAAAC CAAATGCTAT TTGCCACTTC CACAAACCTT 
TTTTATCTTC CATATGAGGG GGAAATATCA CCATCTAATA CTAATATTTG GAAAACAAAC 
TTGCCTAGAG ATGAAATTAA ACTAATTGAA AACATTTGCT GGACACTGAT GGATCATCTA 
GGATATCCAA AGTTTATGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176672.1
CDS	1549..5172
Translation	MAFMFTEHLLFLTLMMCSFSTCEESVSNYSEWAVFTDDIQWLKSQKIQDFKLNRRLHPNLYFDAGDIQTLKQKSRTSHLHIFRAIKSAVTIMLSNPSYYLPPPKHAEFAAKWNEIYGNNLPPLALYCLLCPEDKVAFEFVMEYMDRMVSYKDWLVENAPGDEVPVGHSLTGFATAFDFLYNLLGNQRKQKYLEKIWIVTEEMYEYSKIRSWGKQLLHNHQATNMIALLIGALVTGVDKGSKANIWKQVVVDVMEKTMFLLKHIVDGSLDEGVAYGSYTSKSVTQYVFLAQRHFNINNFDNNWLKMHFWFYYATLLPGYQRTVGIADSNYNWFYGPESQLVFLDKFILQNGAGNWLAQQIRKHRPKDGPMVPSTAQRWSTLHTEYIWYDPTLTPQPPVDFGTAKMHTFPNWGVVTYGGGLPNTQTNTFVSFKSGKLGGRAVYDIVHFQPYSWIDGWRSFNPGHEHPDQNSFTFAPNGQVFVSEALYGPKLSHLNNVLVFAPSPSSQCNQPWEGQLGECAQWLKWTGEEVGDAAGEVITAAQHGDRMFVSGEAVSAYSSAMRLKSVYRALLLLNSQTLLVVDHIERQETSPINSVSAFFHNLDIDFKYIPYKFMNRYNGAMMDVWDAHYKMFWFDHHGNSPVANIQEAEQAAEFKKRWTQFVNVTFHMESTITRIAYVFYGPYVNVSSCRFIDSSSSGLQISLHVNSTEHSVSVVTDYQNLKSRFSYLGFGGFASVANQGQITRFGLGTQEIVNPVRHDKVNFPFGFKFNIAVGFILCISLVILTFQWRFYLSFRKLMRCVLILVIALWFIELLDVWSTCTQPICAKWTRTEAKANEKVMISEGHHVDLPNVIITSLPGSGAEILKQLFFNSSDFLYIRIPTAYMDIPETEFEIDSFVDACEWKVSDIRSGHFHLLRGWLQSLVQDTKLHLQNIHLHETSRSKLAQYFTTNKDKKRKLKRRESLQDQRSRIKGPFDRDAEYIRALRRHLVYYPSARPVLSLSSGSWTLKLHFFQEVLGTSMRALYIVRDPRAWIYSVLYGSKPSLYSLKNVPEHLAKLFKIEEGKSKCNSNSGYAFEYESLKKELEISQSNAISLLSHLWVANTAAALRINTDLLPTNYHLVKFEDIVHFPQKTTERIFAFLGIPLSPASLNQMLFATSTNLFYLPYEGEISPSNTNIWKTNLPRDEIKLIENICWTLMDHLGYPKFMD

Target ORF information:

RefSeq Version	XM_017321183.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dermatan sulfate epimerase-like (Dsel), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321183.1

ORF Insert Sequence:

ATGGCGTTTA TGTTTACAGA ACATTTACTA TTTTTAACAT TGATGATGTG TAGTTTTTCT 
ACTTGTGAAG AATCTGTGAG CAATTATTCT GAATGGGCAG TTTTCACAGA CGATATACAA 
TGGCTTAAGT CACAGAAAAT ACAAGATTTC AAACTCAACC GAAGACTTCA TCCAAATTTA 
TATTTTGATG CTGGAGATAT ACAAACATTG AAACAAAAGT CTCGTACAAG CCATTTGCAT 
ATTTTTAGAG CTATCAAAAG TGCAGTGACA ATTATGCTGT CCAATCCATC ATACTACCTA 
CCTCCACCCA AGCATGCTGA GTTTGCTGCC AAGTGGAATG AAATTTATGG TAATAATCTT 
CCTCCTTTAG CATTGTATTG TTTATTATGC CCAGAAGACA AGGTTGCCTT TGAATTTGTT 
ATGGAATACA TGGATCGGAT GGTTAGCTAC AAAGACTGGC TAGTTGAGAA TGCACCAGGG 
GATGAGGTTC CAGTTGGCCA TTCTTTAACA GGTTTTGCCA CTGCCTTTGA CTTTTTATAT 
AATCTATTAG GTAATCAGCG TAAACAAAAA TACCTAGAAA AAATTTGGAT TGTTACTGAG 
GAAATGTATG AATATTCCAA GATTCGATCA TGGGGCAAAC AACTTCTTCA TAACCATCAA 
GCTACAAATA TGATAGCTTT ACTCATAGGG GCCTTGGTTA CTGGAGTAGA TAAAGGATCT 
AAAGCAAACA TATGGAAACA AGTTGTTGTT GATGTGATGG AAAAGACTAT GTTTCTCTTG 
AAGCATATTG TAGATGGCTC ATTGGATGAA GGTGTGGCCT ATGGAAGCTA TACCTCAAAA 
TCAGTTACAC AGTATGTTTT TTTGGCACAA CGCCATTTTA ACATCAACAA CTTTGATAAT 
AACTGGCTAA AAATGCATTT TTGGTTTTAT TATGCTACAC TTTTGCCAGG CTATCAAAGA 
ACTGTAGGCA TAGCAGATTC CAATTATAAT TGGTTTTATG GTCCAGAGAG CCAGCTAGTT 
TTCTTGGATA AGTTCATTTT ACAGAATGGA GCTGGAAATT GGTTAGCTCA GCAAATTAGA 
AAGCATCGAC CTAAGGATGG ACCAATGGTT CCTTCCACTG CTCAGCGGTG GAGTACTCTT 
CATACTGAAT ACATCTGGTA TGATCCAACA CTCACCCCAC AGCCTCCTGT TGATTTTGGC 
ACTGCAAAAA TGCACACATT TCCTAACTGG GGTGTCGTGA CTTATGGGGG TGGGCTGCCA 
AACACCCAGA CCAATACCTT TGTGTCTTTT AAATCTGGGA AACTGGGAGG ACGAGCTGTG 
TATGACATAG TTCACTTTCA GCCATATTCC TGGATTGATG GATGGAGAAG CTTTAACCCA 
GGACATGAAC ATCCAGATCA AAATTCATTT ACTTTCGCTC CTAATGGGCA GGTATTCGTT 
TCTGAGGCTC TTTATGGACC AAAATTGAGC CACCTTAACA ACGTATTGGT GTTTGCCCCA 
TCACCATCAA GTCAATGTAA TCAGCCCTGG GAAGGTCAAC TGGGAGAATG TGCACAGTGG 
CTCAAGTGGA CTGGGGAAGA GGTTGGTGAT GCAGCTGGGG AAGTTATTAC TGCTGCTCAA 
CATGGTGATA GGATGTTTGT GAGTGGGGAA GCAGTGTCTG CTTATTCTTC TGCCATGAGA 
CTGAAAAGTG TCTATCGTGC TTTACTTCTT TTAAATTCAC AAACTCTGCT TGTTGTCGAT 
CATATTGAAA GGCAAGAAAC TTCCCCAATA AATTCTGTCA GTGCCTTCTT TCATAATTTG 
GATATTGATT TTAAATACAT CCCATACAAG TTTATGAATA GATATAATGG TGCCATGATG 
GATGTGTGGG ATGCACACTA TAAAATGTTT TGGTTTGATC ACCATGGCAA CAGTCCTGTG 
GCTAATATAC AGGAAGCAGA ACAGGCTGCT GAATTTAAGA AACGGTGGAC ACAGTTTGTT 
AATGTTACAT TTCATATGGA ATCCACAATC ACAAGAATTG CTTATGTATT TTATGGGCCA 
TATGTCAATG TTTCCAGCTG CAGATTTATT GATAGTTCCA GTTCTGGACT TCAGATTTCT 
TTACATGTCA ACAGTACTGA ACATAGTGTG TCTGTTGTAA CTGACTATCA AAACCTTAAA 
AGCAGATTCA GTTACCTGGG ATTTGGTGGT TTTGCCAGTG TGGCTAATCA AGGACAGATA 
ACCAGATTTG GTTTGGGTAC TCAAGAAATA GTAAACCCTG TAAGACATGA TAAAGTTAAT 
TTCCCCTTTG GGTTTAAATT TAATATAGCA GTTGGATTCA TTTTGTGTAT TAGTTTGGTT 
ATTTTAACTT TTCAATGGCG GTTTTACCTT TCCTTTAGAA AGCTAATGCG CTGTGTATTA 
ATACTTGTTA TTGCCTTGTG GTTTATTGAG CTTCTGGATG TATGGAGTAC ATGCACTCAG 
CCCATCTGTG CAAAATGGAC AAGGACTGAA GCTAAGGCAA ATGAGAAGGT CATGATTTCT 
GAAGGGCATC ATGTGGATCT TCCTAATGTT ATTATTACCT CACTCCCTGG TTCAGGAGCT 
GAAATTCTCA AACAGCTTTT TTTCAACAGC AGTGATTTTC TCTACATCAG AATTCCTACA 
GCCTACATGG ATATCCCTGA AACTGAATTT GAAATTGACT CATTTGTAGA TGCTTGTGAG 
TGGAAAGTAT CAGATATCCG CAGTGGGCAC TTTCATCTTC TTCGAGGGTG GCTGCAGTCT 
TTGGTCCAGG ATACAAAACT TCACTTGCAA AACATCCATC TACATGAAAC CAGTAGGAGT 
AAACTGGCCC AATATTTTAC AACTAATAAG GACAAAAAGC GAAAATTAAA AAGAAGGGAG 
TCTTTGCAAG ATCAAAGAAG TAGAATAAAA GGACCATTTG ATAGAGATGC TGAATATATT 
AGGGCTTTAA GAAGACACCT TGTTTATTAC CCAAGTGCAC GTCCTGTGCT CAGCTTAAGT 
AGTGGTAGCT GGACATTGAA GCTTCATTTT TTTCAGGAAG TTTTAGGAAC TTCAATGCGG 
GCATTGTACA TAGTAAGAGA CCCTCGAGCT TGGATCTATT CAGTGCTATA TGGTAGTAAA 
CCAAGTCTTT ATTCTTTGAA GAATGTACCA GAGCACTTAG CAAAATTGTT TAAAATAGAG 
GAAGGTAAAA GCAAATGTAA TTCGAATTCT GGCTATGCTT TTGAGTATGA ATCACTGAAG 
AAAGAATTAG AAATATCCCA ATCAAATGCT ATCTCCTTAT TATCTCATTT GTGGGTAGCA 
AACACTGCAG CAGCCTTGAG AATAAATACA GATTTGCTGC CTACCAATTA CCATCTGGTC 
AAGTTTGAAG ATATTGTTCA TTTTCCTCAG AAGACTACTG AAAGGATTTT TGCTTTCCTT 
GGCATTCCTT TGTCTCCTGC TAGTTTAAAC CAAATGCTAT TTGCCACTTC CACAAACCTT 
TTTTATCTTC CATATGAGGG GGAAATATCA CCATCTAATA CTAATATTTG GAAAACAAAC 
TTGCCTAGAG ATGAAATTAA ACTAATTGAA AACATTTGCT GGACACTGAT GGATCATCTA 
GGATATCCAA AGTTTATGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis.
PloS one10(10)e0140279(2015)
Stachtea XN,Tykesson E,van Kuppevelt TH,Feinstein R,Malmstr?m A,Reijmers RM,Maccarana M

Biological functions of iduronic acid in chondroitin/dermatan sulfate.
The FEBS journal280(10)2431-46(2013 May)
Thelin MA,Bartolini B,Axelsson J,Gustafsson R,Tykesson E,Pera E,Oldberg ?,Maccarana M,Malmstrom A

Iduronic acid in chondroitin/dermatan sulfate affects directional migration of aortic smooth muscle cells.
PloS one8(7)e66704(2013)
Bartolini B,Thelin MA,Svensson L,Ghiselli G,van Kuppevelt TH,Malmstr?m A,Maccarana M

Mouse development is not obviously affected by the absence of dermatan sulfate epimerase 2 in spite of a modified brain dermatan sulfate composition.
Glycobiology22(7)1007-16(2012 Jul)
Bartolini B,Thelin MA,Rauch U,Feinstein R,Oldberg A,Malmstr?m A,Maccarana M

Dermatan sulfate epimerase 2 is the predominant isozyme in the formation of the chondroitin sulfate/dermatan sulfate hybrid structure in postnatal developing mouse brain.
Glycobiology21(5)565-74(2011 May)
Akatsu C,Mizumoto S,Kaneiwa T,Maccarana M,Malmstr?m A,Yamada S,Sugahara K

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Dsel cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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