Rc3h2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rc3h2
Entrez Gene ID	319817
Full Name	ring finger and CCCH-type zinc finger domains 2
Synonyms	2900024N03Rik,9430019J22Rik,D930043C02Rik,Mnab,Rnf164
General protein information	Preferred Names ring finger and CCCH-type zinc finger domains 2 Names roquin-2 RING-type E3 ubiquitin transferase Roquin-2 membrane associated DNA binding protein membrane-associated nucleic acid binding protein ring finger and CCCH-type zinc finger domain-containing protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 B

mRNA and Protein(s)

mRNA	Protein	Name
XM_006498124.4	XP_006498187.1	roquin-2 isoform X1
XM_006498124.3	XP_006498187.1	roquin-2 isoform X1
NM_001290642.1	NP_001277571.1	roquin-2 isoform b
NM_001100591.1	NP_001094061.1	roquin-2 isoform a

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RC3H2	XP_520240.3
Canis lupus familiaris (dog)	RC3H2	XP_003639362.1
Rattus norvegicus (Norway rat)	Rc3h2	NP_001101309.1
	RC3H2	XP_001086022.2
Homo sapiens (human)	RC3H2	NP_001094058.1
Bos taurus (cattle)	RC3H2	XP_002691620.3
Gallus gallus (chicken)	RC3H2	XP_415394.2
Mus musculus (house mouse)	Rc3h2	NP_001094061.1
Danio rerio (zebrafish)	rc3h2	XP_005155680.1

Gene Ontology
Bibliography

Related articles in PubMed

Binding of NUFIP2 to Roquin promotes recognition and regulation of ICOS mRNA.
Rehage N, Davydova E, Conrad C, Behrens G, Maiser A, Stehklein JE, Brenner S, Klein J, Jeridi A, Hoffmann A, Lee E, Dianzani U, Willemsen R, Feederle R, Reiche K, Hackerm?ller J, Leonhardt H, Sharma S, Niessing D, Heissmeyer V
Nature communications9(1)299(2018 Jan)

Roquin Suppresses the PI3K-mTOR Signaling Pathway to Inhibit T Helper Cell Differentiation and Conversion of Treg to Tfr Cells.
Essig K, Hu D, Guimaraes JC, Alterauge D, Edelmann S, Raj T, Kranich J, Behrens G, Heiseke A, Floess S, Klein J, Maiser A, Marschall S, Hrab? de Angelis M, Leonhardt H, Calkhoven CF, Noessner E, Brocker T, Huehn J, Krug AB, Zavolan M, Baumjohann D, Heissmeyer V
Immunity47(6)1067-1082.e12(2017 Dec)

Conserved expression of ultra-conserved noncoding RNA in mammalian nervous system.
Zhou J, Wang R, Zhang J, Zhu L, Liu W, Lu S, Chen P, Li H, Yin B, Yuan J, Qiang B, Shu P, Peng X
Biochimica et biophysica acta1860(12)1159-1168(2017 Dec)

Roquin Paralogs Differentially Regulate Functional NKT Cell Subsets.
Drees C, Vahl JC, Bortoluzzi S, Heger KD, Fischer JC, Wunderlich FT, Peschel C, Schmidt-Supprian M
Journal of immunology (Baltimore, Md. : 1950)198(7)2747-2759(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Roquin-mediated control of PI3K-mTOR signaling prevents autoimmunity by restraining activation and differentiation of conventional T cells and specialization of Treg cells.
Title: Roquin Suppresses the PI3K-mTOR Signaling Pathway to Inhibit T Helper Cell Differentiation and Conversion of Treg to Tfr Cells.

Study demonstrates that the constitutive decay element folds into an RNA stem-loop motif that is specifically recognized by Roquin and Roquin2. Binding of Roquin initiates degradation of TNF-alpha mRNA and limits TNF-alpha production in macrophages.
Title: Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs.

Roquin-1 and Roquin-2 proteins redundantly repressed the mRNA of inducible costimulator (Icos) and identified the Ox40 costimulatory receptor as another shared mRNA target.
Title: Roquin paralogs 1 and 2 redundantly repress the Icos and Ox40 costimulator mRNAs and control follicular helper T cell differentiation.

RING-deficient Roquin-1 failed to localize to stress granules and allowed Roquin-2 to compensate in the repression of ICOS and Tfh cells.
Title: Roquin-2 shares functions with its paralog Roquin-1 in the repression of mRNAs controlling T follicular helper cells and systemic inflammation.

Characterization of the human RC3H2 ortholog and observation of conserved sequences in mouse.
Title: A human gene coding for a membrane-associated nucleic acid-binding protein.

The following Rc3h2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rc3h2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13941	XM_006498124.4 Latest version!	Mus musculus ring finger and CCCH-type zinc finger domains 2 (Rc3h2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu13941	NM_001100591.1 Latest version!	Mus musculus ring finger and CCCH-type zinc finger domains 2 (Rc3h2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu16976	NM_001290642.1 Latest version!	Mus musculus ring finger and CCCH-type zinc finger domains 2 (Rc3h2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu13941	XM_006498124.3	Mus musculus ring finger and CCCH-type zinc finger domains 2 (Rc3h2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rc3h2 ( XM_006498124.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498187.1 Mus musculus ring finger and CCCH-type zinc finger domains 2 (Rc3h2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13941
Clone ID Related Accession (Same CDS sequence)	XM_006498124.4 , XM_006498124.3 , NM_001100591.1
Accession Version	XM_006498124.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roquin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498124.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGC AGGCAGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACAGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGCGTAGAGG ACTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AAGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTAGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTATTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTAAA GGAGGAATTT CGGAGTTACG AGGCACTACG CAGAGAACAT 
GATGCCCAGA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TGTATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAGGAAT TGACAATTGT TTTGCAGCGA 
ACTGGTGACC CTGCTAATTT AAATAGGCTG AGACCTCATT TAGAGCTTCT TGCAAACATA 
GACCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TTGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG AAAAGGCCAT 
GAGACACCTC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAAGTACC GATTAAGGAA CAAAAAGATG AGCGCGACTG TAAGAACATT TCCTCTTCTG 
AATAAAGTTG GTGTAAACAG CACTGTCACA ACCACAGCCG GAAATGTCAT TTCTGTCATA 
GGAAGTACTG AAACAACTGG GAAAATTGTT GCAAGTACAA ATGGAATTTC AAATACAGAA 
AGCAGTGTTT CTCAGCTAAT CCCACGAGGC ACTGACAGTG CAGTAAGGAC TTTGGAGACT 
GTGAAGAAAG TCGGGAAGGT TGGCACTAAT GCTCAGAATG CTGGGCCCTC TGCAGAGTCC 
GTGTCTGAAA ATAAAATTGG TTCTCCACCC AAGACTCCTG TAAGTAATGC AGCAGCTACC 
TCCGCTGGGC CCTCTAATTT TGGAACAGAG CTGAATTCCC TGCCTCCCAA ATCCAGCCCA 
TTTCTAACTA GAGTTCCAGT ATATCCTCAG CATTCTGAAA GCATTCAGTA TTTTCAAGAT 
CCAAGGACTC AGATACCTTT TGAAGTCCCA CAATACCCAC AAACAGGATA CTACCCACCA 
CCTCCAACGG TACCAGCTGG TGTGACTCCC TGTGTTCCTC GCTTTGTGAG GTCCAGTAAT 
GTTCCAGAGT CCTCCCTCCC ACCTGCTTCC ATGCCATATG CTGATCATTA CAGTACATTC 
TCCCCTCGAG ATCGAATGAA TTCTTCTCCT TACCAACCTC CTCCTCCGCA GCAGTATGGA 
CCAGTTCCTC CAGTACCCTC TGGAATGTAT GCTCCGGTGT ATGACAGCAG GCGCATCTGG 
CGCCCAGCTA TGTACCAACG AGATGACATT ATTAGAAGCA ATTCTTTACC TCCAATGGAT 
GTGATGCACT CATCTGTCTA TCAGACATCT TTACGGGAAA GATACAACTC ATTAGATGGA 
TATTATTCAG TGGCTTGTCA GCCACCGAAT GACCCAAGGA CAACTGTGCC TTTACCAAGG 
GAACCTTGTG GTCATTTGAA GACCAGTTGT GAGGAACAGT TAAGAAGAAA GCCAGATCAG 
TGGACACAGT ATCATACCCA GAAAACACCT GTCTCTTCAA CTCTTCCTGT GGCCACACAG 
TCACCAACAC CACCTTCTCC TCTATTCAGT GTAGACTTCC GATCGGATTT CTCTGAGAGT 
GTGAGTGGCG CAAAATTTGA GGAAGACCAT CTTTCCCATT ATTCTCCCTG GTCTTGTGGT 
ACCATAGGCT CTTGTATAAA TGCCATTGAT TCGGAGCCCA AAGACGTAAT TGCTAATTCA 
AATGCTGTAT TGATGGACCT GGACAGTGGG GATGTAAAGA GAAGAGTGCA TTTATTTGAA 
GCTCAGAGAA GGACAAAAGA AGAAGACCCA ATTATTCCCT TTAGTGACGG ACCCATTATC 
TCAAAATGGG GTGCAATTTC CCGATCCTCC CGAACAGGTT ACCATACAAC AGATCCCGTC 
CAGGCAACTG CTTCCCAAGG AAGTGCAACT AAGCCCATCA GCGTATCAGA TTATGTCCCT 
TACGTGAATG CTGTCGATTC AAGGTGGAGT TCATATGGCA ACGATGCTAC ATCATCAGCA 
CACTATATTG AACGGGACAG ATTCATTGTT ACTGATTTAT CTGGTCATAG AAAGCATTCT 
AGTACCGGAG ACCTTTTGAG CATTGAACTT CAGCAGGCCA AGAGTAACTC ATTACTTCTT 
CAGAGGGAAG CTAATGCTCT GGCCATGCAA CAAAAGTGGA ATTCCCTGGA TGAAGGCCGT 
CACCTTACTT TAAATCTTCT AAGCAAGGAA ATTGAACTAA GAAATGGAGA GAATGACTAC 
ACAGAAGACA CTGTAGATAC AAAGCCTGAT AGGGATATTG AGTTAGAGCT TTCAGCCCTT 
GATACTGATG AACCTGATGG CCAGAGTGAA CAAATTGAAG AAATTTTGGA CATACAACTT 
GGTATCAGTT CTCAAAATGA CCAGTTGCTC AATGGAACAG CAGTGGAAAA TGGTCATCCA 
GCCCAGCAGC ACCAGAAGGA CCCAGGAAAG CCGAAGAGAC AGAGTTTAGG TGAAGACCAT 
GTGATTCTGG AGGAGCAAAA ACCAATTCTG CCGGTAACAT CTTGCTTTAG CCAGCCACGT 
CCAATGTCTA TTAGCAGTGC AAGCTGCCTC CCCATCACCA CATCTGTCAG TGTTGGCAAC 
CTCATTCTGA AAACTCACGT TATGTCTGAA GATAAAAACG ACTTTTTAAA ACCTATTGCA 
AATGGGAAGA TGGTTAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498187.1
CDS	1671..5234
Translation	MPVQAAQWTEFLSCPICYNEFDENVHKPISLGCSHTVCKTCLNKLHRKACPFDQTAINTDIDVLPVNFALLQLVGAQVPDHQSIKLSNLGENKHYEVAKKCVEDLALYLKPLSGGKGVASLNQSALSRPMQRKLVTLVNCQLVEEEGRVRAMRAARSLGERTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRSYEALRREHDAQIVHIAMEAGLRISPEQWSSLLYGDLAHKSHMQSIIDKLQSPESFAKSVQELTIVLQRTGDPANLNRLRPHLELLANIDPNPDAVSPTWEQLENAMVAVKTVVHGLVDFIQNYSRKGHETPQPQPNSKYKTSMCRDLRQQGGCPRGTNCTFAHSQEELEKYRLRNKKMSATVRTFPLLNKVGVNSTVTTTAGNVISVIGSTETTGKIVASTNGISNTESSVSQLIPRGTDSAVRTLETVKKVGKVGTNAQNAGPSAESVSENKIGSPPKTPVSNAAATSAGPSNFGTELNSLPPKSSPFLTRVPVYPQHSESIQYFQDPRTQIPFEVPQYPQTGYYPPPPTVPAGVTPCVPRFVRSSNVPESSLPPASMPYADHYSTFSPRDRMNSSPYQPPPPQQYGPVPPVPSGMYAPVYDSRRIWRPAMYQRDDIIRSNSLPPMDVMHSSVYQTSLRERYNSLDGYYSVACQPPNDPRTTVPLPREPCGHLKTSCEEQLRRKPDQWTQYHTQKTPVSSTLPVATQSPTPPSPLFSVDFRSDFSESVSGAKFEEDHLSHYSPWSCGTIGSCINAIDSEPKDVIANSNAVLMDLDSGDVKRRVHLFEAQRRTKEEDPIIPFSDGPIISKWGAISRSSRTGYHTTDPVQATASQGSATKPISVSDYVPYVNAVDSRWSSYGNDATSSAHYIERDRFIVTDLSGHRKHSSTGDLLSIELQQAKSNSLLLQREANALAMQQKWNSLDEGRHLTLNLLSKEIELRNGENDYTEDTVDTKPDRDIELELSALDTDEPDGQSEQIEEILDIQLGISSQNDQLLNGTAVENGHPAQQHQKDPGKPKRQSLGEDHVILEEQKPILPVTSCFSQPRPMSISSASCLPITTSVSVGNLILKTHVMSEDKNDFLKPIANGKMVNS

Target ORF information:

RefSeq Version	XM_006498124.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger and CCCH-type zinc finger domains 2 (Rc3h2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498124.4

ORF Insert Sequence:

ATGCCTGTGC AGGCAGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACAGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGCGTAGAGG ACTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AAGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTAGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTATTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTAAA GGAGGAATTT CGGAGTTACG AGGCACTACG CAGAGAACAT 
GATGCCCAGA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TGTATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAGGAAT TGACAATTGT TTTGCAGCGA 
ACTGGTGACC CTGCTAATTT AAATAGGCTG AGACCTCATT TAGAGCTTCT TGCAAACATA 
GACCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TTGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG AAAAGGCCAT 
GAGACACCTC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAAGTACC GATTAAGGAA CAAAAAGATG AGCGCGACTG TAAGAACATT TCCTCTTCTG 
AATAAAGTTG GTGTAAACAG CACTGTCACA ACCACAGCCG GAAATGTCAT TTCTGTCATA 
GGAAGTACTG AAACAACTGG GAAAATTGTT GCAAGTACAA ATGGAATTTC AAATACAGAA 
AGCAGTGTTT CTCAGCTAAT CCCACGAGGC ACTGACAGTG CAGTAAGGAC TTTGGAGACT 
GTGAAGAAAG TCGGGAAGGT TGGCACTAAT GCTCAGAATG CTGGGCCCTC TGCAGAGTCC 
GTGTCTGAAA ATAAAATTGG TTCTCCACCC AAGACTCCTG TAAGTAATGC AGCAGCTACC 
TCCGCTGGGC CCTCTAATTT TGGAACAGAG CTGAATTCCC TGCCTCCCAA ATCCAGCCCA 
TTTCTAACTA GAGTTCCAGT ATATCCTCAG CATTCTGAAA GCATTCAGTA TTTTCAAGAT 
CCAAGGACTC AGATACCTTT TGAAGTCCCA CAATACCCAC AAACAGGATA CTACCCACCA 
CCTCCAACGG TACCAGCTGG TGTGACTCCC TGTGTTCCTC GCTTTGTGAG GTCCAGTAAT 
GTTCCAGAGT CCTCCCTCCC ACCTGCTTCC ATGCCATATG CTGATCATTA CAGTACATTC 
TCCCCTCGAG ATCGAATGAA TTCTTCTCCT TACCAACCTC CTCCTCCGCA GCAGTATGGA 
CCAGTTCCTC CAGTACCCTC TGGAATGTAT GCTCCGGTGT ATGACAGCAG GCGCATCTGG 
CGCCCAGCTA TGTACCAACG AGATGACATT ATTAGAAGCA ATTCTTTACC TCCAATGGAT 
GTGATGCACT CATCTGTCTA TCAGACATCT TTACGGGAAA GATACAACTC ATTAGATGGA 
TATTATTCAG TGGCTTGTCA GCCACCGAAT GACCCAAGGA CAACTGTGCC TTTACCAAGG 
GAACCTTGTG GTCATTTGAA GACCAGTTGT GAGGAACAGT TAAGAAGAAA GCCAGATCAG 
TGGACACAGT ATCATACCCA GAAAACACCT GTCTCTTCAA CTCTTCCTGT GGCCACACAG 
TCACCAACAC CACCTTCTCC TCTATTCAGT GTAGACTTCC GATCGGATTT CTCTGAGAGT 
GTGAGTGGCG CAAAATTTGA GGAAGACCAT CTTTCCCATT ATTCTCCCTG GTCTTGTGGT 
ACCATAGGCT CTTGTATAAA TGCCATTGAT TCGGAGCCCA AAGACGTAAT TGCTAATTCA 
AATGCTGTAT TGATGGACCT GGACAGTGGG GATGTAAAGA GAAGAGTGCA TTTATTTGAA 
GCTCAGAGAA GGACAAAAGA AGAAGACCCA ATTATTCCCT TTAGTGACGG ACCCATTATC 
TCAAAATGGG GTGCAATTTC CCGATCCTCC CGAACAGGTT ACCATACAAC AGATCCCGTC 
CAGGCAACTG CTTCCCAAGG AAGTGCAACT AAGCCCATCA GCGTATCAGA TTATGTCCCT 
TACGTGAATG CTGTCGATTC AAGGTGGAGT TCATATGGCA ACGATGCTAC ATCATCAGCA 
CACTATATTG AACGGGACAG ATTCATTGTT ACTGATTTAT CTGGTCATAG AAAGCATTCT 
AGTACCGGAG ACCTTTTGAG CATTGAACTT CAGCAGGCCA AGAGTAACTC ATTACTTCTT 
CAGAGGGAAG CTAATGCTCT GGCCATGCAA CAAAAGTGGA ATTCCCTGGA TGAAGGCCGT 
CACCTTACTT TAAATCTTCT AAGCAAGGAA ATTGAACTAA GAAATGGAGA GAATGACTAC 
ACAGAAGACA CTGTAGATAC AAAGCCTGAT AGGGATATTG AGTTAGAGCT TTCAGCCCTT 
GATACTGATG AACCTGATGG CCAGAGTGAA CAAATTGAAG AAATTTTGGA CATACAACTT 
GGTATCAGTT CTCAAAATGA CCAGTTGCTC AATGGAACAG CAGTGGAAAA TGGTCATCCA 
GCCCAGCAGC ACCAGAAGGA CCCAGGAAAG CCGAAGAGAC AGAGTTTAGG TGAAGACCAT 
GTGATTCTGG AGGAGCAAAA ACCAATTCTG CCGGTAACAT CTTGCTTTAG CCAGCCACGT 
CCAATGTCTA TTAGCAGTGC AAGCTGCCTC CCCATCACCA CATCTGTCAG TGTTGGCAAC 
CTCATTCTGA AAACTCACGT TATGTCTGAA GATAAAAACG ACTTTTTAAA ACCTATTGCA 
AATGGGAAGA TGGTTAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rc3h2 ( NM_001100591.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001094061.1 Mus musculus ring finger and CCCH-type zinc finger domains 2 (Rc3h2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13941
Clone ID Related Accession (Same CDS sequence)	XM_006498124.4 , XM_006498124.3 , NM_001100591.1
Accession Version	NM_001100591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	roquin-2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK053071.1, BC117952.1, BC117951.1, AL929572.10 and BM898966.1. On or before Aug 11, 2007 this sequence version replaced XM_925271.3, XM_130233.8. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC117951.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGTGC AGGCAGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACAGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGCGTAGAGG ACTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AAGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTAGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTATTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTAAA GGAGGAATTT CGGAGTTACG AGGCACTACG CAGAGAACAT 
GATGCCCAGA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TGTATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAGGAAT TGACAATTGT TTTGCAGCGA 
ACTGGTGACC CTGCTAATTT AAATAGGCTG AGACCTCATT TAGAGCTTCT TGCAAACATA 
GACCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TTGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG AAAAGGCCAT 
GAGACACCTC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAAGTACC GATTAAGGAA CAAAAAGATG AGCGCGACTG TAAGAACATT TCCTCTTCTG 
AATAAAGTTG GTGTAAACAG CACTGTCACA ACCACAGCCG GAAATGTCAT TTCTGTCATA 
GGAAGTACTG AAACAACTGG GAAAATTGTT GCAAGTACAA ATGGAATTTC AAATACAGAA 
AGCAGTGTTT CTCAGCTAAT CCCACGAGGC ACTGACAGTG CAGTAAGGAC TTTGGAGACT 
GTGAAGAAAG TCGGGAAGGT TGGCACTAAT GCTCAGAATG CTGGGCCCTC TGCAGAGTCC 
GTGTCTGAAA ATAAAATTGG TTCTCCACCC AAGACTCCTG TAAGTAATGC AGCAGCTACC 
TCCGCTGGGC CCTCTAATTT TGGAACAGAG CTGAATTCCC TGCCTCCCAA ATCCAGCCCA 
TTTCTAACTA GAGTTCCAGT ATATCCTCAG CATTCTGAAA GCATTCAGTA TTTTCAAGAT 
CCAAGGACTC AGATACCTTT TGAAGTCCCA CAATACCCAC AAACAGGATA CTACCCACCA 
CCTCCAACGG TACCAGCTGG TGTGACTCCC TGTGTTCCTC GCTTTGTGAG GTCCAGTAAT 
GTTCCAGAGT CCTCCCTCCC ACCTGCTTCC ATGCCATATG CTGATCATTA CAGTACATTC 
TCCCCTCGAG ATCGAATGAA TTCTTCTCCT TACCAACCTC CTCCTCCGCA GCAGTATGGA 
CCAGTTCCTC CAGTACCCTC TGGAATGTAT GCTCCGGTGT ATGACAGCAG GCGCATCTGG 
CGCCCAGCTA TGTACCAACG AGATGACATT ATTAGAAGCA ATTCTTTACC TCCAATGGAT 
GTGATGCACT CATCTGTCTA TCAGACATCT TTACGGGAAA GATACAACTC ATTAGATGGA 
TATTATTCAG TGGCTTGTCA GCCACCGAAT GACCCAAGGA CAACTGTGCC TTTACCAAGG 
GAACCTTGTG GTCATTTGAA GACCAGTTGT GAGGAACAGT TAAGAAGAAA GCCAGATCAG 
TGGACACAGT ATCATACCCA GAAAACACCT GTCTCTTCAA CTCTTCCTGT GGCCACACAG 
TCACCAACAC CACCTTCTCC TCTATTCAGT GTAGACTTCC GATCGGATTT CTCTGAGAGT 
GTGAGTGGCG CAAAATTTGA GGAAGACCAT CTTTCCCATT ATTCTCCCTG GTCTTGTGGT 
ACCATAGGCT CTTGTATAAA TGCCATTGAT TCGGAGCCCA AAGACGTAAT TGCTAATTCA 
AATGCTGTAT TGATGGACCT GGACAGTGGG GATGTAAAGA GAAGAGTGCA TTTATTTGAA 
GCTCAGAGAA GGACAAAAGA AGAAGACCCA ATTATTCCCT TTAGTGACGG ACCCATTATC 
TCAAAATGGG GTGCAATTTC CCGATCCTCC CGAACAGGTT ACCATACAAC AGATCCCGTC 
CAGGCAACTG CTTCCCAAGG AAGTGCAACT AAGCCCATCA GCGTATCAGA TTATGTCCCT 
TACGTGAATG CTGTCGATTC AAGGTGGAGT TCATATGGCA ACGATGCTAC ATCATCAGCA 
CACTATATTG AACGGGACAG ATTCATTGTT ACTGATTTAT CTGGTCATAG AAAGCATTCT 
AGTACCGGAG ACCTTTTGAG CATTGAACTT CAGCAGGCCA AGAGTAACTC ATTACTTCTT 
CAGAGGGAAG CTAATGCTCT GGCCATGCAA CAAAAGTGGA ATTCCCTGGA TGAAGGCCGT 
CACCTTACTT TAAATCTTCT AAGCAAGGAA ATTGAACTAA GAAATGGAGA GAATGACTAC 
ACAGAAGACA CTGTAGATAC AAAGCCTGAT AGGGATATTG AGTTAGAGCT TTCAGCCCTT 
GATACTGATG AACCTGATGG CCAGAGTGAA CAAATTGAAG AAATTTTGGA CATACAACTT 
GGTATCAGTT CTCAAAATGA CCAGTTGCTC AATGGAACAG CAGTGGAAAA TGGTCATCCA 
GCCCAGCAGC ACCAGAAGGA CCCAGGAAAG CCGAAGAGAC AGAGTTTAGG TGAAGACCAT 
GTGATTCTGG AGGAGCAAAA ACCAATTCTG CCGGTAACAT CTTGCTTTAG CCAGCCACGT 
CCAATGTCTA TTAGCAGTGC AAGCTGCCTC CCCATCACCA CATCTGTCAG TGTTGGCAAC 
CTCATTCTGA AAACTCACGT TATGTCTGAA GATAAAAACG ACTTTTTAAA ACCTATTGCA 
AATGGGAAGA TGGTTAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094061.1
CDS	240..3803
Translation	MPVQAAQWTEFLSCPICYNEFDENVHKPISLGCSHTVCKTCLNKLHRKACPFDQTAINTDIDVLPVNFALLQLVGAQVPDHQSIKLSNLGENKHYEVAKKCVEDLALYLKPLSGGKGVASLNQSALSRPMQRKLVTLVNCQLVEEEGRVRAMRAARSLGERTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRSYEALRREHDAQIVHIAMEAGLRISPEQWSSLLYGDLAHKSHMQSIIDKLQSPESFAKSVQELTIVLQRTGDPANLNRLRPHLELLANIDPNPDAVSPTWEQLENAMVAVKTVVHGLVDFIQNYSRKGHETPQPQPNSKYKTSMCRDLRQQGGCPRGTNCTFAHSQEELEKYRLRNKKMSATVRTFPLLNKVGVNSTVTTTAGNVISVIGSTETTGKIVASTNGISNTESSVSQLIPRGTDSAVRTLETVKKVGKVGTNAQNAGPSAESVSENKIGSPPKTPVSNAAATSAGPSNFGTELNSLPPKSSPFLTRVPVYPQHSESIQYFQDPRTQIPFEVPQYPQTGYYPPPPTVPAGVTPCVPRFVRSSNVPESSLPPASMPYADHYSTFSPRDRMNSSPYQPPPPQQYGPVPPVPSGMYAPVYDSRRIWRPAMYQRDDIIRSNSLPPMDVMHSSVYQTSLRERYNSLDGYYSVACQPPNDPRTTVPLPREPCGHLKTSCEEQLRRKPDQWTQYHTQKTPVSSTLPVATQSPTPPSPLFSVDFRSDFSESVSGAKFEEDHLSHYSPWSCGTIGSCINAIDSEPKDVIANSNAVLMDLDSGDVKRRVHLFEAQRRTKEEDPIIPFSDGPIISKWGAISRSSRTGYHTTDPVQATASQGSATKPISVSDYVPYVNAVDSRWSSYGNDATSSAHYIERDRFIVTDLSGHRKHSSTGDLLSIELQQAKSNSLLLQREANALAMQQKWNSLDEGRHLTLNLLSKEIELRNGENDYTEDTVDTKPDRDIELELSALDTDEPDGQSEQIEEILDIQLGISSQNDQLLNGTAVENGHPAQQHQKDPGKPKRQSLGEDHVILEEQKPILPVTSCFSQPRPMSISSASCLPITTSVSVGNLILKTHVMSEDKNDFLKPIANGKMVNS

Target ORF information:

RefSeq Version	NM_001100591.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger and CCCH-type zinc finger domains 2 (Rc3h2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100591.1

ORF Insert Sequence:

ATGCCTGTGC AGGCAGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACAGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGCGTAGAGG ACTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AAGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTAGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTATTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTAAA GGAGGAATTT CGGAGTTACG AGGCACTACG CAGAGAACAT 
GATGCCCAGA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TGTATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAGGAAT TGACAATTGT TTTGCAGCGA 
ACTGGTGACC CTGCTAATTT AAATAGGCTG AGACCTCATT TAGAGCTTCT TGCAAACATA 
GACCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TTGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG AAAAGGCCAT 
GAGACACCTC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAAGTACC GATTAAGGAA CAAAAAGATG AGCGCGACTG TAAGAACATT TCCTCTTCTG 
AATAAAGTTG GTGTAAACAG CACTGTCACA ACCACAGCCG GAAATGTCAT TTCTGTCATA 
GGAAGTACTG AAACAACTGG GAAAATTGTT GCAAGTACAA ATGGAATTTC AAATACAGAA 
AGCAGTGTTT CTCAGCTAAT CCCACGAGGC ACTGACAGTG CAGTAAGGAC TTTGGAGACT 
GTGAAGAAAG TCGGGAAGGT TGGCACTAAT GCTCAGAATG CTGGGCCCTC TGCAGAGTCC 
GTGTCTGAAA ATAAAATTGG TTCTCCACCC AAGACTCCTG TAAGTAATGC AGCAGCTACC 
TCCGCTGGGC CCTCTAATTT TGGAACAGAG CTGAATTCCC TGCCTCCCAA ATCCAGCCCA 
TTTCTAACTA GAGTTCCAGT ATATCCTCAG CATTCTGAAA GCATTCAGTA TTTTCAAGAT 
CCAAGGACTC AGATACCTTT TGAAGTCCCA CAATACCCAC AAACAGGATA CTACCCACCA 
CCTCCAACGG TACCAGCTGG TGTGACTCCC TGTGTTCCTC GCTTTGTGAG GTCCAGTAAT 
GTTCCAGAGT CCTCCCTCCC ACCTGCTTCC ATGCCATATG CTGATCATTA CAGTACATTC 
TCCCCTCGAG ATCGAATGAA TTCTTCTCCT TACCAACCTC CTCCTCCGCA GCAGTATGGA 
CCAGTTCCTC CAGTACCCTC TGGAATGTAT GCTCCGGTGT ATGACAGCAG GCGCATCTGG 
CGCCCAGCTA TGTACCAACG AGATGACATT ATTAGAAGCA ATTCTTTACC TCCAATGGAT 
GTGATGCACT CATCTGTCTA TCAGACATCT TTACGGGAAA GATACAACTC ATTAGATGGA 
TATTATTCAG TGGCTTGTCA GCCACCGAAT GACCCAAGGA CAACTGTGCC TTTACCAAGG 
GAACCTTGTG GTCATTTGAA GACCAGTTGT GAGGAACAGT TAAGAAGAAA GCCAGATCAG 
TGGACACAGT ATCATACCCA GAAAACACCT GTCTCTTCAA CTCTTCCTGT GGCCACACAG 
TCACCAACAC CACCTTCTCC TCTATTCAGT GTAGACTTCC GATCGGATTT CTCTGAGAGT 
GTGAGTGGCG CAAAATTTGA GGAAGACCAT CTTTCCCATT ATTCTCCCTG GTCTTGTGGT 
ACCATAGGCT CTTGTATAAA TGCCATTGAT TCGGAGCCCA AAGACGTAAT TGCTAATTCA 
AATGCTGTAT TGATGGACCT GGACAGTGGG GATGTAAAGA GAAGAGTGCA TTTATTTGAA 
GCTCAGAGAA GGACAAAAGA AGAAGACCCA ATTATTCCCT TTAGTGACGG ACCCATTATC 
TCAAAATGGG GTGCAATTTC CCGATCCTCC CGAACAGGTT ACCATACAAC AGATCCCGTC 
CAGGCAACTG CTTCCCAAGG AAGTGCAACT AAGCCCATCA GCGTATCAGA TTATGTCCCT 
TACGTGAATG CTGTCGATTC AAGGTGGAGT TCATATGGCA ACGATGCTAC ATCATCAGCA 
CACTATATTG AACGGGACAG ATTCATTGTT ACTGATTTAT CTGGTCATAG AAAGCATTCT 
AGTACCGGAG ACCTTTTGAG CATTGAACTT CAGCAGGCCA AGAGTAACTC ATTACTTCTT 
CAGAGGGAAG CTAATGCTCT GGCCATGCAA CAAAAGTGGA ATTCCCTGGA TGAAGGCCGT 
CACCTTACTT TAAATCTTCT AAGCAAGGAA ATTGAACTAA GAAATGGAGA GAATGACTAC 
ACAGAAGACA CTGTAGATAC AAAGCCTGAT AGGGATATTG AGTTAGAGCT TTCAGCCCTT 
GATACTGATG AACCTGATGG CCAGAGTGAA CAAATTGAAG AAATTTTGGA CATACAACTT 
GGTATCAGTT CTCAAAATGA CCAGTTGCTC AATGGAACAG CAGTGGAAAA TGGTCATCCA 
GCCCAGCAGC ACCAGAAGGA CCCAGGAAAG CCGAAGAGAC AGAGTTTAGG TGAAGACCAT 
GTGATTCTGG AGGAGCAAAA ACCAATTCTG CCGGTAACAT CTTGCTTTAG CCAGCCACGT 
CCAATGTCTA TTAGCAGTGC AAGCTGCCTC CCCATCACCA CATCTGTCAG TGTTGGCAAC 
CTCATTCTGA AAACTCACGT TATGTCTGAA GATAAAAACG ACTTTTTAAA ACCTATTGCA 
AATGGGAAGA TGGTTAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rc3h2 ( NM_001290642.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277571.1 Mus musculus ring finger and CCCH-type zinc finger domains 2 (Rc3h2), transcript variant 2, mRNA.

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CloneID	OMu16976
Clone ID Related Accession (Same CDS sequence)	NM_001290642.1
Accession Version	NM_001290642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	roquin-2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK053071.1, AL929572.10 and BM898966.1. On Mar 15, 2014 this sequence version replaced XM_006498125.1. Transcript Variant: This variant (2) uses an alternate splice site that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK053071.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGTGC AGGCAGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACAGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGCGTAGAGG ACTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AAGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTAGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTATTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTAAA GGAGGAATTT CGGAGTTACG AGGCACTACG CAGAGAACAT 
GATGCCCAGA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TGTATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAGGAAT TGACAATTGT TTTGCAGCGA 
ACTGGTGACC CTGCTAATTT AAATAGGCTG AGACCTCATT TAGAGCTTCT TGCAAACATA 
GACCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TTGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG AAAAGGCCAT 
GAGACACCTC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAAGTACC GATTAAGGAA CAAAAAGATG AGCGCGACTG TAAGAACATT TCCTCTTCTG 
AATAAAGTTG GTGTAAACAG CACTGTCACA ACCACAGCCG GAAATGTCAT TTCTGTCATA 
GGAAGTACTG AAACAACTGG GAAAATTGTT GCAAGTACAA ATGGAATTTC AAATACAGAA 
AGCAGTGTTT CTCAGCTAAT CCCACGAGGC ACTGACAGTG CAGTAAGGAC TTTGGAGACT 
GTGAAGAAAG TCGGGAAGGT TGGCACTAAT GCTCAGAATG CTGGGCCCTC TGCAGAGTCC 
GTGTCTGAAA ATAAAATTGG TTCTCCACCC AAGACTCCTG TAAGTAATGC AGCAGCTACC 
TCCGCTGGGC CCTCTAATTT TGGAACAGAG CTGAATTCCC TGCCTCCCAA ATCCAGCCCA 
TTTCTAACTA GAGTTCCAGT ATATCCTCAG CATTCTGAAA GCATTCAGTA TTTTCAAGAT 
CCAAGGACTC AGATACCTTT TGAAGTCCCA CAATACCCAC AAACAGGATA CTACCCACCA 
CCTCCAACGG TACCAGCTGG TGTGACTCCC TGTGTTCCTC GCTTTGTGAG GTCCAGTAAT 
GTTCCAGAGT CCTCCCTCCC ACCTGCTTCC ATGCCATATG CTGATCATTA CAGTACATTC 
TCCCCTCGAG ATCGAATGAA TTCTTCTCCT TACCAACCTC CTCCTCCGCA GCAGTATGGA 
CCAGTTCCTC CAGTACCCTC TGGAATGTAT GCTCCGGTGT ATGACAGCAG GCGCATCTGG 
CGCCCAGCTA TGTACCAACG AGATGACATT ATTAGAAGCA ATTCTTTACC TCCAATGGAT 
GTGATGCACT CATCTGTCTA TCAGACATCT TTACGGGAAA GATACAACTC ATTAGATGGA 
TATTATTCAG TGGCTTGTCA GCCACCGAAT GACCCAAGGA CAACTGTGCC TTTACCAAGG 
GAACCTTGTG GTCATTTGAA GACCAGTTGT GAGGAACAGT TAAGAAGAAA GCCAGATCAG 
TGGACACAGT ATCATACCCA GAAAACACCT GTCTCTTCAA CTCTTCCTGT GGCCACACAG 
TCACCAACAC CACCTTCTCC TCTATTCAGT GTAGACTTCC GATCGGATTT CTCTGAGAGT 
GTGAGTGGCG CAAAATTTGA GGAAGACCAT CTTTCCCATT ATTCTCCCTG GTCTTGTGGT 
ACCATAGGCT CTTGTATAAA TGCCATTGAT TCGGAGCCCA AAGACGTAAT TGCTAATTCA 
AATGCTGTAT TGATGGACCT GGACAGTGGG GATGTAAAGA GAAGAGTGCA TTTATTTGAA 
GCTCAGAGAA GGACAAAAGA AGAAGACCCA ATTATTCCCT TTAGTGACGG ACCCATTATC 
TCAAAATGGG GTGCAATTTC CCGATCCTCC CGAACAGGTT ACCATACAAC AGATCCCGTC 
CAGGCAACTG CTTCCCAAGG AAGTGCAACT AAGCCCATCA GCGTATCAGA TTATGTCCCT 
TACGTGAATG CTGTCGATTC AAGGTGGAGT TCATATGGCA ACGATGCTAC ATCATCAGCA 
CACTATATTG AACGGGACAG ATTCATTGTT ACTGATTTAT CTGGTCATAG AAAGCATTCT 
AGTACCGGAG ACCTTTTGAG CATTGAACTT CAGCAGGCCA AGAGTAACTC ATTACTTCTT 
CAGAGGGAAG CTAATGCTCT GGCCATGCAA CAAAAGTGGA ATTCCCTGGA TGAAGGCCGT 
CACCTTACTT TAAATCTTCT AAGCAAGGAA ATTGAACTAA GAAATGGAGA GAATGACTAC 
ACAGAAGACA CTGTAGATAC AAAGCCTGAT AGGGATATTG AGTTAGAGCT TTCAGCCCTT 
GATACTGATG AACCTGATGG CCAGAGTGAA CAAATTGAAG AAATTTTGGA CATACAACTT 
GGTATCAGTT CTCAAAATGA CCAGTTGCTC AATGGAACAG CAGTGGAAAA TGGTCATCCA 
GCCCAGCAGC ACCAGAAGGA CCCAGGAAAG CCGAAGAGAC AGAGTTTAGG GAGGAGCAAA 
AACCAATTCT GCCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277571.1
CDS	240..3617
Translation	MPVQAAQWTEFLSCPICYNEFDENVHKPISLGCSHTVCKTCLNKLHRKACPFDQTAINTDIDVLPVNFALLQLVGAQVPDHQSIKLSNLGENKHYEVAKKCVEDLALYLKPLSGGKGVASLNQSALSRPMQRKLVTLVNCQLVEEEGRVRAMRAARSLGERTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRSYEALRREHDAQIVHIAMEAGLRISPEQWSSLLYGDLAHKSHMQSIIDKLQSPESFAKSVQELTIVLQRTGDPANLNRLRPHLELLANIDPNPDAVSPTWEQLENAMVAVKTVVHGLVDFIQNYSRKGHETPQPQPNSKYKTSMCRDLRQQGGCPRGTNCTFAHSQEELEKYRLRNKKMSATVRTFPLLNKVGVNSTVTTTAGNVISVIGSTETTGKIVASTNGISNTESSVSQLIPRGTDSAVRTLETVKKVGKVGTNAQNAGPSAESVSENKIGSPPKTPVSNAAATSAGPSNFGTELNSLPPKSSPFLTRVPVYPQHSESIQYFQDPRTQIPFEVPQYPQTGYYPPPPTVPAGVTPCVPRFVRSSNVPESSLPPASMPYADHYSTFSPRDRMNSSPYQPPPPQQYGPVPPVPSGMYAPVYDSRRIWRPAMYQRDDIIRSNSLPPMDVMHSSVYQTSLRERYNSLDGYYSVACQPPNDPRTTVPLPREPCGHLKTSCEEQLRRKPDQWTQYHTQKTPVSSTLPVATQSPTPPSPLFSVDFRSDFSESVSGAKFEEDHLSHYSPWSCGTIGSCINAIDSEPKDVIANSNAVLMDLDSGDVKRRVHLFEAQRRTKEEDPIIPFSDGPIISKWGAISRSSRTGYHTTDPVQATASQGSATKPISVSDYVPYVNAVDSRWSSYGNDATSSAHYIERDRFIVTDLSGHRKHSSTGDLLSIELQQAKSNSLLLQREANALAMQQKWNSLDEGRHLTLNLLSKEIELRNGENDYTEDTVDTKPDRDIELELSALDTDEPDGQSEQIEEILDIQLGISSQNDQLLNGTAVENGHPAQQHQKDPGKPKRQSLGRSKNQFCR

Target ORF information:

RefSeq Version	NM_001290642.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger and CCCH-type zinc finger domains 2 (Rc3h2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290642.1

ORF Insert Sequence:

ATGCCTGTGC AGGCAGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACAGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGCGTAGAGG ACTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AAGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTAGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTATTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTAAA GGAGGAATTT CGGAGTTACG AGGCACTACG CAGAGAACAT 
GATGCCCAGA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TGTATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAGGAAT TGACAATTGT TTTGCAGCGA 
ACTGGTGACC CTGCTAATTT AAATAGGCTG AGACCTCATT TAGAGCTTCT TGCAAACATA 
GACCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TTGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG AAAAGGCCAT 
GAGACACCTC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAAGTACC GATTAAGGAA CAAAAAGATG AGCGCGACTG TAAGAACATT TCCTCTTCTG 
AATAAAGTTG GTGTAAACAG CACTGTCACA ACCACAGCCG GAAATGTCAT TTCTGTCATA 
GGAAGTACTG AAACAACTGG GAAAATTGTT GCAAGTACAA ATGGAATTTC AAATACAGAA 
AGCAGTGTTT CTCAGCTAAT CCCACGAGGC ACTGACAGTG CAGTAAGGAC TTTGGAGACT 
GTGAAGAAAG TCGGGAAGGT TGGCACTAAT GCTCAGAATG CTGGGCCCTC TGCAGAGTCC 
GTGTCTGAAA ATAAAATTGG TTCTCCACCC AAGACTCCTG TAAGTAATGC AGCAGCTACC 
TCCGCTGGGC CCTCTAATTT TGGAACAGAG CTGAATTCCC TGCCTCCCAA ATCCAGCCCA 
TTTCTAACTA GAGTTCCAGT ATATCCTCAG CATTCTGAAA GCATTCAGTA TTTTCAAGAT 
CCAAGGACTC AGATACCTTT TGAAGTCCCA CAATACCCAC AAACAGGATA CTACCCACCA 
CCTCCAACGG TACCAGCTGG TGTGACTCCC TGTGTTCCTC GCTTTGTGAG GTCCAGTAAT 
GTTCCAGAGT CCTCCCTCCC ACCTGCTTCC ATGCCATATG CTGATCATTA CAGTACATTC 
TCCCCTCGAG ATCGAATGAA TTCTTCTCCT TACCAACCTC CTCCTCCGCA GCAGTATGGA 
CCAGTTCCTC CAGTACCCTC TGGAATGTAT GCTCCGGTGT ATGACAGCAG GCGCATCTGG 
CGCCCAGCTA TGTACCAACG AGATGACATT ATTAGAAGCA ATTCTTTACC TCCAATGGAT 
GTGATGCACT CATCTGTCTA TCAGACATCT TTACGGGAAA GATACAACTC ATTAGATGGA 
TATTATTCAG TGGCTTGTCA GCCACCGAAT GACCCAAGGA CAACTGTGCC TTTACCAAGG 
GAACCTTGTG GTCATTTGAA GACCAGTTGT GAGGAACAGT TAAGAAGAAA GCCAGATCAG 
TGGACACAGT ATCATACCCA GAAAACACCT GTCTCTTCAA CTCTTCCTGT GGCCACACAG 
TCACCAACAC CACCTTCTCC TCTATTCAGT GTAGACTTCC GATCGGATTT CTCTGAGAGT 
GTGAGTGGCG CAAAATTTGA GGAAGACCAT CTTTCCCATT ATTCTCCCTG GTCTTGTGGT 
ACCATAGGCT CTTGTATAAA TGCCATTGAT TCGGAGCCCA AAGACGTAAT TGCTAATTCA 
AATGCTGTAT TGATGGACCT GGACAGTGGG GATGTAAAGA GAAGAGTGCA TTTATTTGAA 
GCTCAGAGAA GGACAAAAGA AGAAGACCCA ATTATTCCCT TTAGTGACGG ACCCATTATC 
TCAAAATGGG GTGCAATTTC CCGATCCTCC CGAACAGGTT ACCATACAAC AGATCCCGTC 
CAGGCAACTG CTTCCCAAGG AAGTGCAACT AAGCCCATCA GCGTATCAGA TTATGTCCCT 
TACGTGAATG CTGTCGATTC AAGGTGGAGT TCATATGGCA ACGATGCTAC ATCATCAGCA 
CACTATATTG AACGGGACAG ATTCATTGTT ACTGATTTAT CTGGTCATAG AAAGCATTCT 
AGTACCGGAG ACCTTTTGAG CATTGAACTT CAGCAGGCCA AGAGTAACTC ATTACTTCTT 
CAGAGGGAAG CTAATGCTCT GGCCATGCAA CAAAAGTGGA ATTCCCTGGA TGAAGGCCGT 
CACCTTACTT TAAATCTTCT AAGCAAGGAA ATTGAACTAA GAAATGGAGA GAATGACTAC 
ACAGAAGACA CTGTAGATAC AAAGCCTGAT AGGGATATTG AGTTAGAGCT TTCAGCCCTT 
GATACTGATG AACCTGATGG CCAGAGTGAA CAAATTGAAG AAATTTTGGA CATACAACTT 
GGTATCAGTT CTCAAAATGA CCAGTTGCTC AATGGAACAG CAGTGGAAAA TGGTCATCCA 
GCCCAGCAGC ACCAGAAGGA CCCAGGAAAG CCGAAGAGAC AGAGTTTAGG GAGGAGCAAA 
AACCAATTCT GCCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rc3h2 ( XM_006498124.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498187.1 Mus musculus ring finger and CCCH-type zinc finger domains 2 (Rc3h2), transcript variant X1, mRNA.

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CloneID	OMu13941
Clone ID Related Accession (Same CDS sequence)	XM_006498124.4 , XM_006498124.3 , NM_001100591.1
Accession Version	XM_006498124.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roquin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498124.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGC AGGCAGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACAGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGCGTAGAGG ACTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AAGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTAGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTATTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTAAA GGAGGAATTT CGGAGTTACG AGGCACTACG CAGAGAACAT 
GATGCCCAGA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TGTATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAGGAAT TGACAATTGT TTTGCAGCGA 
ACTGGTGACC CTGCTAATTT AAATAGGCTG AGACCTCATT TAGAGCTTCT TGCAAACATA 
GACCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TTGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG AAAAGGCCAT 
GAGACACCTC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAAGTACC GATTAAGGAA CAAAAAGATG AGCGCGACTG TAAGAACATT TCCTCTTCTG 
AATAAAGTTG GTGTAAACAG CACTGTCACA ACCACAGCCG GAAATGTCAT TTCTGTCATA 
GGAAGTACTG AAACAACTGG GAAAATTGTT GCAAGTACAA ATGGAATTTC AAATACAGAA 
AGCAGTGTTT CTCAGCTAAT CCCACGAGGC ACTGACAGTG CAGTAAGGAC TTTGGAGACT 
GTGAAGAAAG TCGGGAAGGT TGGCACTAAT GCTCAGAATG CTGGGCCCTC TGCAGAGTCC 
GTGTCTGAAA ATAAAATTGG TTCTCCACCC AAGACTCCTG TAAGTAATGC AGCAGCTACC 
TCCGCTGGGC CCTCTAATTT TGGAACAGAG CTGAATTCCC TGCCTCCCAA ATCCAGCCCA 
TTTCTAACTA GAGTTCCAGT ATATCCTCAG CATTCTGAAA GCATTCAGTA TTTTCAAGAT 
CCAAGGACTC AGATACCTTT TGAAGTCCCA CAATACCCAC AAACAGGATA CTACCCACCA 
CCTCCAACGG TACCAGCTGG TGTGACTCCC TGTGTTCCTC GCTTTGTGAG GTCCAGTAAT 
GTTCCAGAGT CCTCCCTCCC ACCTGCTTCC ATGCCATATG CTGATCATTA CAGTACATTC 
TCCCCTCGAG ATCGAATGAA TTCTTCTCCT TACCAACCTC CTCCTCCGCA GCAGTATGGA 
CCAGTTCCTC CAGTACCCTC TGGAATGTAT GCTCCGGTGT ATGACAGCAG GCGCATCTGG 
CGCCCAGCTA TGTACCAACG AGATGACATT ATTAGAAGCA ATTCTTTACC TCCAATGGAT 
GTGATGCACT CATCTGTCTA TCAGACATCT TTACGGGAAA GATACAACTC ATTAGATGGA 
TATTATTCAG TGGCTTGTCA GCCACCGAAT GACCCAAGGA CAACTGTGCC TTTACCAAGG 
GAACCTTGTG GTCATTTGAA GACCAGTTGT GAGGAACAGT TAAGAAGAAA GCCAGATCAG 
TGGACACAGT ATCATACCCA GAAAACACCT GTCTCTTCAA CTCTTCCTGT GGCCACACAG 
TCACCAACAC CACCTTCTCC TCTATTCAGT GTAGACTTCC GATCGGATTT CTCTGAGAGT 
GTGAGTGGCG CAAAATTTGA GGAAGACCAT CTTTCCCATT ATTCTCCCTG GTCTTGTGGT 
ACCATAGGCT CTTGTATAAA TGCCATTGAT TCGGAGCCCA AAGACGTAAT TGCTAATTCA 
AATGCTGTAT TGATGGACCT GGACAGTGGG GATGTAAAGA GAAGAGTGCA TTTATTTGAA 
GCTCAGAGAA GGACAAAAGA AGAAGACCCA ATTATTCCCT TTAGTGACGG ACCCATTATC 
TCAAAATGGG GTGCAATTTC CCGATCCTCC CGAACAGGTT ACCATACAAC AGATCCCGTC 
CAGGCAACTG CTTCCCAAGG AAGTGCAACT AAGCCCATCA GCGTATCAGA TTATGTCCCT 
TACGTGAATG CTGTCGATTC AAGGTGGAGT TCATATGGCA ACGATGCTAC ATCATCAGCA 
CACTATATTG AACGGGACAG ATTCATTGTT ACTGATTTAT CTGGTCATAG AAAGCATTCT 
AGTACCGGAG ACCTTTTGAG CATTGAACTT CAGCAGGCCA AGAGTAACTC ATTACTTCTT 
CAGAGGGAAG CTAATGCTCT GGCCATGCAA CAAAAGTGGA ATTCCCTGGA TGAAGGCCGT 
CACCTTACTT TAAATCTTCT AAGCAAGGAA ATTGAACTAA GAAATGGAGA GAATGACTAC 
ACAGAAGACA CTGTAGATAC AAAGCCTGAT AGGGATATTG AGTTAGAGCT TTCAGCCCTT 
GATACTGATG AACCTGATGG CCAGAGTGAA CAAATTGAAG AAATTTTGGA CATACAACTT 
GGTATCAGTT CTCAAAATGA CCAGTTGCTC AATGGAACAG CAGTGGAAAA TGGTCATCCA 
GCCCAGCAGC ACCAGAAGGA CCCAGGAAAG CCGAAGAGAC AGAGTTTAGG TGAAGACCAT 
GTGATTCTGG AGGAGCAAAA ACCAATTCTG CCGGTAACAT CTTGCTTTAG CCAGCCACGT 
CCAATGTCTA TTAGCAGTGC AAGCTGCCTC CCCATCACCA CATCTGTCAG TGTTGGCAAC 
CTCATTCTGA AAACTCACGT TATGTCTGAA GATAAAAACG ACTTTTTAAA ACCTATTGCA 
AATGGGAAGA TGGTTAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498187.1
CDS	709..4272
Translation	MPVQAAQWTEFLSCPICYNEFDENVHKPISLGCSHTVCKTCLNKLHRKACPFDQTAINTDIDVLPVNFALLQLVGAQVPDHQSIKLSNLGENKHYEVAKKCVEDLALYLKPLSGGKGVASLNQSALSRPMQRKLVTLVNCQLVEEEGRVRAMRAARSLGERTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRSYEALRREHDAQIVHIAMEAGLRISPEQWSSLLYGDLAHKSHMQSIIDKLQSPESFAKSVQELTIVLQRTGDPANLNRLRPHLELLANIDPNPDAVSPTWEQLENAMVAVKTVVHGLVDFIQNYSRKGHETPQPQPNSKYKTSMCRDLRQQGGCPRGTNCTFAHSQEELEKYRLRNKKMSATVRTFPLLNKVGVNSTVTTTAGNVISVIGSTETTGKIVASTNGISNTESSVSQLIPRGTDSAVRTLETVKKVGKVGTNAQNAGPSAESVSENKIGSPPKTPVSNAAATSAGPSNFGTELNSLPPKSSPFLTRVPVYPQHSESIQYFQDPRTQIPFEVPQYPQTGYYPPPPTVPAGVTPCVPRFVRSSNVPESSLPPASMPYADHYSTFSPRDRMNSSPYQPPPPQQYGPVPPVPSGMYAPVYDSRRIWRPAMYQRDDIIRSNSLPPMDVMHSSVYQTSLRERYNSLDGYYSVACQPPNDPRTTVPLPREPCGHLKTSCEEQLRRKPDQWTQYHTQKTPVSSTLPVATQSPTPPSPLFSVDFRSDFSESVSGAKFEEDHLSHYSPWSCGTIGSCINAIDSEPKDVIANSNAVLMDLDSGDVKRRVHLFEAQRRTKEEDPIIPFSDGPIISKWGAISRSSRTGYHTTDPVQATASQGSATKPISVSDYVPYVNAVDSRWSSYGNDATSSAHYIERDRFIVTDLSGHRKHSSTGDLLSIELQQAKSNSLLLQREANALAMQQKWNSLDEGRHLTLNLLSKEIELRNGENDYTEDTVDTKPDRDIELELSALDTDEPDGQSEQIEEILDIQLGISSQNDQLLNGTAVENGHPAQQHQKDPGKPKRQSLGEDHVILEEQKPILPVTSCFSQPRPMSISSASCLPITTSVSVGNLILKTHVMSEDKNDFLKPIANGKMVNS

Target ORF information:

RefSeq Version	XM_006498124.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger and CCCH-type zinc finger domains 2 (Rc3h2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498124.3

ORF Insert Sequence:

ATGCCTGTGC AGGCAGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACAGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGCGTAGAGG ACTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AAGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTAGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTATTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTAAA GGAGGAATTT CGGAGTTACG AGGCACTACG CAGAGAACAT 
GATGCCCAGA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TGTATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAGGAAT TGACAATTGT TTTGCAGCGA 
ACTGGTGACC CTGCTAATTT AAATAGGCTG AGACCTCATT TAGAGCTTCT TGCAAACATA 
GACCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TTGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG AAAAGGCCAT 
GAGACACCTC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAAGTACC GATTAAGGAA CAAAAAGATG AGCGCGACTG TAAGAACATT TCCTCTTCTG 
AATAAAGTTG GTGTAAACAG CACTGTCACA ACCACAGCCG GAAATGTCAT TTCTGTCATA 
GGAAGTACTG AAACAACTGG GAAAATTGTT GCAAGTACAA ATGGAATTTC AAATACAGAA 
AGCAGTGTTT CTCAGCTAAT CCCACGAGGC ACTGACAGTG CAGTAAGGAC TTTGGAGACT 
GTGAAGAAAG TCGGGAAGGT TGGCACTAAT GCTCAGAATG CTGGGCCCTC TGCAGAGTCC 
GTGTCTGAAA ATAAAATTGG TTCTCCACCC AAGACTCCTG TAAGTAATGC AGCAGCTACC 
TCCGCTGGGC CCTCTAATTT TGGAACAGAG CTGAATTCCC TGCCTCCCAA ATCCAGCCCA 
TTTCTAACTA GAGTTCCAGT ATATCCTCAG CATTCTGAAA GCATTCAGTA TTTTCAAGAT 
CCAAGGACTC AGATACCTTT TGAAGTCCCA CAATACCCAC AAACAGGATA CTACCCACCA 
CCTCCAACGG TACCAGCTGG TGTGACTCCC TGTGTTCCTC GCTTTGTGAG GTCCAGTAAT 
GTTCCAGAGT CCTCCCTCCC ACCTGCTTCC ATGCCATATG CTGATCATTA CAGTACATTC 
TCCCCTCGAG ATCGAATGAA TTCTTCTCCT TACCAACCTC CTCCTCCGCA GCAGTATGGA 
CCAGTTCCTC CAGTACCCTC TGGAATGTAT GCTCCGGTGT ATGACAGCAG GCGCATCTGG 
CGCCCAGCTA TGTACCAACG AGATGACATT ATTAGAAGCA ATTCTTTACC TCCAATGGAT 
GTGATGCACT CATCTGTCTA TCAGACATCT TTACGGGAAA GATACAACTC ATTAGATGGA 
TATTATTCAG TGGCTTGTCA GCCACCGAAT GACCCAAGGA CAACTGTGCC TTTACCAAGG 
GAACCTTGTG GTCATTTGAA GACCAGTTGT GAGGAACAGT TAAGAAGAAA GCCAGATCAG 
TGGACACAGT ATCATACCCA GAAAACACCT GTCTCTTCAA CTCTTCCTGT GGCCACACAG 
TCACCAACAC CACCTTCTCC TCTATTCAGT GTAGACTTCC GATCGGATTT CTCTGAGAGT 
GTGAGTGGCG CAAAATTTGA GGAAGACCAT CTTTCCCATT ATTCTCCCTG GTCTTGTGGT 
ACCATAGGCT CTTGTATAAA TGCCATTGAT TCGGAGCCCA AAGACGTAAT TGCTAATTCA 
AATGCTGTAT TGATGGACCT GGACAGTGGG GATGTAAAGA GAAGAGTGCA TTTATTTGAA 
GCTCAGAGAA GGACAAAAGA AGAAGACCCA ATTATTCCCT TTAGTGACGG ACCCATTATC 
TCAAAATGGG GTGCAATTTC CCGATCCTCC CGAACAGGTT ACCATACAAC AGATCCCGTC 
CAGGCAACTG CTTCCCAAGG AAGTGCAACT AAGCCCATCA GCGTATCAGA TTATGTCCCT 
TACGTGAATG CTGTCGATTC AAGGTGGAGT TCATATGGCA ACGATGCTAC ATCATCAGCA 
CACTATATTG AACGGGACAG ATTCATTGTT ACTGATTTAT CTGGTCATAG AAAGCATTCT 
AGTACCGGAG ACCTTTTGAG CATTGAACTT CAGCAGGCCA AGAGTAACTC ATTACTTCTT 
CAGAGGGAAG CTAATGCTCT GGCCATGCAA CAAAAGTGGA ATTCCCTGGA TGAAGGCCGT 
CACCTTACTT TAAATCTTCT AAGCAAGGAA ATTGAACTAA GAAATGGAGA GAATGACTAC 
ACAGAAGACA CTGTAGATAC AAAGCCTGAT AGGGATATTG AGTTAGAGCT TTCAGCCCTT 
GATACTGATG AACCTGATGG CCAGAGTGAA CAAATTGAAG AAATTTTGGA CATACAACTT 
GGTATCAGTT CTCAAAATGA CCAGTTGCTC AATGGAACAG CAGTGGAAAA TGGTCATCCA 
GCCCAGCAGC ACCAGAAGGA CCCAGGAAAG CCGAAGAGAC AGAGTTTAGG TGAAGACCAT 
GTGATTCTGG AGGAGCAAAA ACCAATTCTG CCGGTAACAT CTTGCTTTAG CCAGCCACGT 
CCAATGTCTA TTAGCAGTGC AAGCTGCCTC CCCATCACCA CATCTGTCAG TGTTGGCAAC 
CTCATTCTGA AAACTCACGT TATGTCTGAA GATAAAAACG ACTTTTTAAA ACCTATTGCA 
AATGGGAAGA TGGTTAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Binding of NUFIP2 to Roquin promotes recognition and regulation of ICOS mRNA.
Nature communications9(1)299(2018 Jan)
Rehage N,Davydova E,Conrad C,Behrens G,Maiser A,Stehklein JE,Brenner S,Klein J,Jeridi A,Hoffmann A,Lee E,Dianzani U,Willemsen R,Feederle R,Reiche K,Hackerm?ller J,Leonhardt H,Sharma S,Niessing D,Heissmeyer V

Roquin Suppresses the PI3K-mTOR Signaling Pathway to Inhibit T Helper Cell Differentiation and Conversion of Treg to Tfr Cells.
Immunity47(6)1067-1082.e12(2017 Dec)
Essig K,Hu D,Guimaraes JC,Alterauge D,Edelmann S,Raj T,Kranich J,Behrens G,Heiseke A,Floess S,Klein J,Maiser A,Marschall S,Hrab? de Angelis M,Leonhardt H,Calkhoven CF,Noessner E,Brocker T,Huehn J,Krug AB,Zavolan M,Baumjohann D,Heissmeyer V

Conserved expression of ultra-conserved noncoding RNA in mammalian nervous system.
Biochimica et biophysica acta1860(12)1159-1168(2017 Dec)
Zhou J,Wang R,Zhang J,Zhu L,Liu W,Lu S,Chen P,Li H,Yin B,Yuan J,Qiang B,Shu P,Peng X

Roquin Paralogs Differentially Regulate Functional NKT Cell Subsets.
Journal of immunology (Baltimore, Md. : 1950)198(7)2747-2759(2017 Apr)
Drees C,Vahl JC,Bortoluzzi S,Heger KD,Fischer JC,Wunderlich FT,Peschel C,Schmidt-Supprian M

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Rc3h2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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