Tigar cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tigar
Entrez Gene ID	319801
Full Name	Trp53 induced glycolysis repulatory phosphatase
Synonyms	9630033F20Rik,AA793651,AI595337,C79710,C85509
General protein information	Preferred Names Trp53 induced glycolysis repulatory phosphatase Names fructose-2,6-bisphosphatase TIGAR TP53-induced glycolysis and apoptosis regulator TP53-induced glycolysis regulatory phosphatase probable fructose-2,6-bisphosphatase TIGAR
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 F3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006506254.1	XP_006506317.1	fructose-2,6-bisphosphatase TIGAR isoform X1
NM_177003.5	NP_795977.1	fructose-2,6-bisphosphatase TIGAR

Pathways from BioSystems

Homologies

	LOC721886	XP_001118082.1
Rattus norvegicus (Norway rat)	RGD1308005	XP_006240250.1
Canis lupus familiaris (dog)	C27H12orf5	XP_543863.3
Gallus gallus (chicken)	C1H12ORF5	XP_417232.1
Xenopus tropicalis (tropical clawed frog)	c12orf5	NP_001120569.1
Homo sapiens (human)	C12orf5	NP_065108.1
Bos taurus (cattle)	C5H12orf5	NP_001069838.1
Mus musculus (house mouse)	9630033F20Rik	NP_795977.1
Pan troglodytes (chimpanzee)	C12H12orf5	XP_522324.2

Gene Ontology
Bibliography

Related articles in PubMed

TIGAR inhibits ischemia/reperfusion-induced inflammatory response of astrocytes.
Chen J, Zhang DM, Feng X, Wang J, Qin YY, Zhang T, Huang Q, Sheng R, Chen Z, Li M, Qin ZH
Neuropharmacology131377-388(2018 Jan)

TIGAR contributes to ischemic tolerance induced by cerebral preconditioning through scavenging of reactive oxygen species and inhibition of apoptosis.
Zhou JH, Zhang TT, Song DD, Xia YF, Qin ZH, Sheng R
Scientific reports627096(2016 Jun)

Jmjd5 functions as a regulator of p53 signaling during mouse embryogenesis.
Ishimura A, Terashima M, Tange S, Suzuki T
Cell and tissue research363(3)723-33(2016 Mar)

Opposing effects of TIGAR- and RAC1-derived ROS on Wnt-driven proliferation in the mouse intestine.
Cheung EC, Lee P, Ceteci F, Nixon C, Blyth K, Sansom OJ, Vousden KH
Genes & development30(1)52-63(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that TIGAR expression changes during development and its expression level may be correlated with the vulnerability of neurons to ischemic injury.
Title: Endogenous level of TIGAR in brain is associated with vulnerability of neurons to ischemic injury.

Although mouse TIGAR expression is clearly induced in the intestines of mice following DNA-damaging stress of ionizing radiation, that was not dependent on p53 or TAp73.
Title: p53- and p73-independent activation of TIGAR expression in vivo.

TIGAR protein expression in brain is increased following ischemia reperfusion injury.
Title: Ischemia/reperfusion-induced upregulation of TIGAR in brain is mediated by SP1 and modulated by ROS and hormones involved in glucose metabolism.

TIGAR protects ischemic brain injury and preserves mitochodrial function.
Title: A TIGAR-regulated metabolic pathway is critical for protection of brain ischemia.

Therefore, we conclude that TIGAR knockdown-induced radiosensitization of glioma cells may be dependent on the inhibition of TRX1 nuclear translocation.
Title: Radiosensitization of glioma cells by TP53-induced glycolysis and apoptosis regulator knockdown is dependent on thioredoxin-1 nuclear translocation.

The following Tigar gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tigar cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13565	NM_177003.5 Latest version!	Mus musculus Trp53 induced glycolysis regulatory phosphatase (Tigar), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu37357	XM_006506254.1 Latest version!	Mus musculus Trp53 induced glycolysis regulatory phosphatase (Tigar), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tigar ( NM_177003.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_795977.1 Mus musculus Trp53 induced glycolysis regulatory phosphatase (Tigar), mRNA.

Price & Availability↑

CloneID	OMu13565
Clone ID Related Accession (Same CDS sequence)	NM_177003.5
Accession Version	NM_177003.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	fructose-2,6-bisphosphatase TIGAR
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163683.4 and AK145896.1. On Apr 10, 2010 this sequence version replaced NM_177003.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK145896.1, AK165614.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGCCGCGCT TCGCCTTGAC CGTTATCCGC CATGGTGAAA CAAGACTTAA TAAGGAGAAA 
ATCATTCAAG GACAAGGCGT AGATGCGCCC CTTTCGGAGA CTGGGTTTCG GCAAGCAGCG 
GCCGCCGGGC AGTTTCTGAG CAATGTGCAG TTTACCCACG CCTTCTCCAG CGATCTCACG 
AGGACTAAGC AGACCATACA TGGCATTTTG GAGAAAAGCA GATTTTGTAA AGACATGGCG 
GTGAAGTACG ACTCCAGGCT CCGAGAAAGG ATGTACGGAG TCGCGGAAGG CAAGCCGCTA 
AGCGAGCTTC GGGCCATGGC CAAAGCCGCT GGGGAAGAGT GCCCCATGTT CACCCCGCCT 
GGAGGAGAGA CAGTTGAGCA GGTAAAGATG CGCGGAAAGG ATTTCTTTGA TTTCATTTGT 
CAGCTAATCC TGGGCAAGGC AGGGCAGAGA GAAAGCGTCC TGCCTGGAGC GCCAGGCAGC 
GGTTTGGAAA GCTCTTTGGC AGAGGTTTTC CCTGTTGGAA AACATGGCAG CTTGGGGGCG 
AATCCCAAAG GTGGCACCCT GGGCTTAGCA GCCAGCATCT TAGTTGTGAG CCATGGCGCT 
TACATGAGAA GCCTCTTTGG TTATTTTCTG AGTGACCTCA GATGCTCGTT GCCAGGAGCG 
AGAGACAAAT TGGAACTTTC CTCCATCACT CCCAACACTG GGATCAGTGT CTTCATCATA 
GACTGTGAGG AAGCACGCCA GCCATCGATT CAGTGCGTTT GTATGAACCT CCAGGAGCAC 
CTGAACGGAG TGACTGAGAA GCAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_795977.1
CDS	86..895
Translation	MPRFALTVIRHGETRLNKEKIIQGQGVDAPLSETGFRQAAAAGQFLSNVQFTHAFSSDLTRTKQTIHGILEKSRFCKDMAVKYDSRLRERMYGVAEGKPLSELRAMAKAAGEECPMFTPPGGETVEQVKMRGKDFFDFICQLILGKAGQRESVLPGAPGSGLESSLAEVFPVGKHGSLGANPKGGTLGLAASILVVSHGAYMRSLFGYFLSDLRCSLPGARDKLELSSITPNTGISVFIIDCEEARQPSIQCVCMNLQEHLNGVTEKQH

Target ORF information:

RefSeq Version	NM_177003.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus Trp53 induced glycolysis regulatory phosphatase (Tigar), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177003.5

ORF Insert Sequence:

ATGCCGCGCT TCGCCTTGAC CGTTATCCGC CATGGTGAAA CAAGACTTAA TAAGGAGAAA 
ATCATTCAAG GACAAGGCGT AGATGCGCCC CTTTCGGAGA CTGGGTTTCG GCAAGCAGCG 
GCCGCCGGGC AGTTTCTGAG CAATGTGCAG TTTACCCACG CCTTCTCCAG CGATCTCACG 
AGGACTAAGC AGACCATACA TGGCATTTTG GAGAAAAGCA GATTTTGTAA AGACATGGCG 
GTGAAGTACG ACTCCAGGCT CCGAGAAAGG ATGTACGGAG TCGCGGAAGG CAAGCCGCTA 
AGCGAGCTTC GGGCCATGGC CAAAGCCGCT GGGGAAGAGT GCCCCATGTT CACCCCGCCT 
GGAGGAGAGA CAGTTGAGCA GGTAAAGATG CGCGGAAAGG ATTTCTTTGA TTTCATTTGT 
CAGCTAATCC TGGGCAAGGC AGGGCAGAGA GAAAGCGTCC TGCCTGGAGC GCCAGGCAGC 
GGTTTGGAAA GCTCTTTGGC AGAGGTTTTC CCTGTTGGAA AACATGGCAG CTTGGGGGCG 
AATCCCAAAG GTGGCACCCT GGGCTTAGCA GCCAGCATCT TAGTTGTGAG CCATGGCGCT 
TACATGAGAA GCCTCTTTGG TTATTTTCTG AGTGACCTCA GATGCTCGTT GCCAGGAGCG 
AGAGACAAAT TGGAACTTTC CTCCATCACT CCCAACACTG GGATCAGTGT CTTCATCATA 
GACTGTGAGG AAGCACGCCA GCCATCGATT CAGTGCGTTT GTATGAACCT CCAGGAGCAC 
CTGAACGGAG TGACTGAGAA GCAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tigar ( XM_006506254.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506317.1 Mus musculus Trp53 induced glycolysis regulatory phosphatase (Tigar), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu37357
Clone ID Related Accession (Same CDS sequence)	XM_006506254.1
Accession Version	XM_006506254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	fructose-2,6-bisphosphatase TIGAR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGA AGTACGACTC CAGGCTCCGA GAAAGGATGT ACGGAGTCGC GGAAGGCAAG 
CCGCTAAGCG AGCTTCGGGC CATGGCCAAA GCCGCTGGGG AAGAGTGCCC CATGTTCACC 
CCGCCTGGAG GAGAGACAGT TGAGCAGGTA AAGATGCGCG GAAAGGATTT CTTTGATTTC 
ATTTGTCAGC TAATCCTGGG CAAGGCAGGG CAGAGAGAAA GCGTCCTGCC TGGAGCGCCA 
GGCAGCGGTT TGGAAAGCTC TTTGGCAGAG GTTTTCCCTG TTGGAAAACA TGGCAGCTTG 
GGGGCGAATC CCAAAGGTGG CACCCTGGGC TTAGCAGCCA GCATCTTAGT TGTGAGCCAT 
GGCGCTTACA TGAGAAGCCT CTTTGGTTAT TTTCTGAGTG ACCTCAGATG CTCGTTGCCA 
GGAGCGAGAG ACAAATTGGA ACTTTCCTCC ATCACTCCCA ACACTGGGAT CAGTGTCTTC 
ATCATAGACT GTGAGGAAGC ACGCCAGCCA TCGATTCAGT GCGTTTGTAT GAACCTCCAG 
GAGCACCTGA ACGGAGTGAC TGAGAAGCAG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506317.1
CDS	200..775
Translation	MAVKYDSRLRERMYGVAEGKPLSELRAMAKAAGEECPMFTPPGGETVEQVKMRGKDFFDFICQLILGKAGQRESVLPGAPGSGLESSLAEVFPVGKHGSLGANPKGGTLGLAASILVVSHGAYMRSLFGYFLSDLRCSLPGARDKLELSSITPNTGISVFIIDCEEARQPSIQCVCMNLQEHLNGVTEKQH

Target ORF information:

RefSeq Version	XM_006506254.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Trp53 induced glycolysis regulatory phosphatase (Tigar), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506254.1

ORF Insert Sequence:

ATGGCGGTGA AGTACGACTC CAGGCTCCGA GAAAGGATGT ACGGAGTCGC GGAAGGCAAG 
CCGCTAAGCG AGCTTCGGGC CATGGCCAAA GCCGCTGGGG AAGAGTGCCC CATGTTCACC 
CCGCCTGGAG GAGAGACAGT TGAGCAGGTA AAGATGCGCG GAAAGGATTT CTTTGATTTC 
ATTTGTCAGC TAATCCTGGG CAAGGCAGGG CAGAGAGAAA GCGTCCTGCC TGGAGCGCCA 
GGCAGCGGTT TGGAAAGCTC TTTGGCAGAG GTTTTCCCTG TTGGAAAACA TGGCAGCTTG 
GGGGCGAATC CCAAAGGTGG CACCCTGGGC TTAGCAGCCA GCATCTTAGT TGTGAGCCAT 
GGCGCTTACA TGAGAAGCCT CTTTGGTTAT TTTCTGAGTG ACCTCAGATG CTCGTTGCCA 
GGAGCGAGAG ACAAATTGGA ACTTTCCTCC ATCACTCCCA ACACTGGGAT CAGTGTCTTC 
ATCATAGACT GTGAGGAAGC ACGCCAGCCA TCGATTCAGT GCGTTTGTAT GAACCTCCAG 
GAGCACCTGA ACGGAGTGAC TGAGAAGCAG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TIGAR inhibits ischemia/reperfusion-induced inflammatory response of astrocytes.
Neuropharmacology131377-388(2018 Jan)
Chen J,Zhang DM,Feng X,Wang J,Qin YY,Zhang T,Huang Q,Sheng R,Chen Z,Li M,Qin ZH

TIGAR contributes to ischemic tolerance induced by cerebral preconditioning through scavenging of reactive oxygen species and inhibition of apoptosis.
Scientific reports627096(2016 Jun)
Zhou JH,Zhang TT,Song DD,Xia YF,Qin ZH,Sheng R

Jmjd5 functions as a regulator of p53 signaling during mouse embryogenesis.
Cell and tissue research363(3)723-33(2016 Mar)
Ishimura A,Terashima M,Tange S,Suzuki T

Opposing effects of TIGAR- and RAC1-derived ROS on Wnt-driven proliferation in the mouse intestine.
Genes & development30(1)52-63(2016 Jan)
Cheung EC,Lee P,Ceteci F,Nixon C,Blyth K,Sansom OJ,Vousden KH

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Tigar cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報