Cep295 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_017313419.1	XP_017168908.1	centrosomal protein of 295 kDa isoform X1
XM_006510409.1	XP_006510472.1	centrosomal protein of 295 kDa isoform X3
XM_017313423.1	XP_017168912.1	centrosomal protein of 295 kDa isoform X10
XM_017313419.2	XP_017168908.1	centrosomal protein of 295 kDa isoform X1
XM_017313422.2	XP_017168911.1	centrosomal protein of 295 kDa isoform X8
XM_011242547.1	XP_011240849.1	centrosomal protein of 295 kDa isoform X2
XM_017313420.1	XP_017168909.1	centrosomal protein of 295 kDa isoform X4
XM_011242548.2	XP_011240850.1	centrosomal protein of 295 kDa isoform X5
XM_006510411.1	XP_006510474.1	centrosomal protein of 295 kDa isoform X6
XM_017313424.1	XP_017168913.1	centrosomal protein of 295 kDa isoform X12
XM_017313421.2	XP_017168910.1	centrosomal protein of 295 kDa isoform X7
XM_030244413.1	XP_030100273.1	centrosomal protein of 295 kDa isoform X10
XM_017313423.2	XP_017168912.1	centrosomal protein of 295 kDa isoform X13
NM_176976.4	NP_795950.2	centrosomal protein of 295 kDa
XM_017313422.1	XP_017168911.1	centrosomal protein of 295 kDa isoform X8
XM_011242550.2	XP_011240852.1	centrosomal protein of 295 kDa isoform X9
XM_017313425.1	XP_017168914.1	centrosomal protein of 295 kDa isoform X13
XM_011242548.3	XP_011240850.1	centrosomal protein of 295 kDa isoform X5
XM_006510411.2	XP_006510474.1	centrosomal protein of 295 kDa isoform X6
XM_030244415.1	XP_030100275.1	centrosomal protein of 295 kDa isoform X11
XM_011242547.2	XP_011240849.1	centrosomal protein of 295 kDa isoform X2
XM_006510409.2	XP_006510472.1	centrosomal protein of 295 kDa isoform X3
XM_030244412.1	XP_030100272.1	centrosomal protein of 295 kDa isoform X9
XM_030244414.1	XP_030100274.1	centrosomal protein of 295 kDa isoform X11
XM_017313421.1	XP_017168910.1	centrosomal protein of 295 kDa isoform X7
XM_006510414.1	XP_006510477.1	centrosomal protein of 295 kDa isoform X11
XM_017313420.2	XP_017168909.1	centrosomal protein of 295 kDa isoform X4
XM_011242550.3	XP_011240852.1	centrosomal protein of 295 kDa isoform X12
XM_006510414.2	XP_006510477.1	centrosomal protein of 295 kDa isoform X14
XM_017313424.2	XP_017168913.1	centrosomal protein of 295 kDa isoform X15

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu82855	XM_017313424.2 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu82856	XM_017313425.1 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu42262	XM_006510409.2 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu42264	XM_006510411.2 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu42267	XM_006510414.2 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu74558	XM_011242547.2 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu74559	XM_011242548.3 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu74561	XM_011242550.3 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu82850	XM_017313419.2 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu82851	XM_017313420.2 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu82852	XM_017313421.2 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu82853	XM_017313422.2 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu82854	XM_017313423.2 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu90201	XM_030244412.1 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu90202	XM_030244413.1 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu90203	XM_030244415.1 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu90203	XM_030244414.1 Latest version!	Mus musculus centrosomal protein 295 (Cep295), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu05807	NM_176976.4 Latest version!	Mus musculus centrosomal protein 295 (Cep295), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
View Old Accession Versions >>
OMu82850	XM_017313419.1	Mus musculus centrosomal protein 295 (Cep295), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu82854	XM_017313423.1	Mus musculus centrosomal protein 295 (Cep295), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu82853	XM_017313422.1	Mus musculus centrosomal protein 295 (Cep295), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu74559	XM_011242548.2	Mus musculus centrosomal protein 295 (Cep295), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu42264	XM_006510411.1	Mus musculus centrosomal protein 295 (Cep295), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu74558	XM_011242547.1	Mus musculus centrosomal protein 295 (Cep295), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu42262	XM_006510409.1	Mus musculus centrosomal protein 295 (Cep295), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu82852	XM_017313421.1	Mus musculus centrosomal protein 295 (Cep295), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu82851	XM_017313420.1	Mus musculus centrosomal protein 295 (Cep295), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu74561	XM_011242550.2	Mus musculus centrosomal protein 295 (Cep295), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu42267	XM_006510414.1	Mus musculus centrosomal protein 295 (Cep295), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu82855	XM_017313424.1	Mus musculus centrosomal protein 295 (Cep295), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

Cep295 ( XM_017313424.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168913.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu82855
Clone ID Related Accession (Same CDS sequence)	XM_017313424.1 , XM_017313424.2
Accession Version	XM_017313424.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGCA AGGAATCCCA CAGATGAGAA TGACTTGGAA 
ATGCAGAAGA GAGAACAGCT CGGAGGGTGG TTTCCTCACA CCCAAGGCCT TACCTGGGGA 
GACGCAGGTC AGGGGAGCGC TAATGGTGAG CAGCCACGTG CAGATGTCCA TGCTGAGCAC 
AATGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG AGTCATTTTC AGAACAGACA GAGCATCTAG AACAAGAATC TACCAATAAG 
CAGGAAGAGA CTGATCCTCT TAGTATTGCA GTCCCTTCAG TTATTTATCA GCAACAGCAT 
TCCTTAGGTG CTCATAACTC TCTGTTGCCC ATGGAGGAAG AAAGTACATC TGATCATACA 
CATGTTCAGC AGATCATGGA CAATGATGTA AATGAAGCAA ATTTGATACC TGACAAAAGA 
GACTTTCAGG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGAGCTCAG AATCTTCATC TGAAAAACAT 
GTTAAGGCAT TATCCACAGG CACAATCTGC TTCCCAGCAC TGACAGCCAA GTCACATTCT 
CCTAACCCAA GGCTGAACCA ACTTGATATT AACCTGGCTC ATGCAACAAC TGAGGGATCT 
GAACAGTCTT TTCAACAGCT TCGACCAGAA TTCTCTTCAC AGGAAAGCCA ACATGCTGAC 
CTACCCAGTA TTTATAGCAT TGAAGCAAGA GGTCCTTCCC AACGCATGGA AAACCAGAAC 
TATTCTGAAA TGTTACAAAA TAAGAAAAAA AGTCTCTCTC TCCAGCCCTC AACAGAGGAT 
TTGACTCCAG CTTGCAGTTC ATCTGATACT GCTCTGTTTG ATCAGTTACA CCTGCAGCAC 
AGCACTCCGT GTGCTTCTGT CTCTAGTGAG TGCTCAGTAA AACTGTTAGA AAGCAGAGAA 
GAAGTGCTGG GATTTGAAGA GCTGTCAAGA AGGGCAGTTA CAATGTCACA AAGACTCACT 
GAAGATGAAA ATGTAGTTCT ACCTATAAAC CCACATGTAG GAAGAGTGGA GAAGGAGGCT 
TCAGTTCAGG GGTCTAATCC ATTGAGTATT CAAAATGAAA AGCCAATTCA GAACTTCATT 
GAAACTGACA CTACAGAAGC TGTAGGAAAT GTATGTCAGC TAGCACAAGC AGAGCACATA 
TTAAAATCCT GTCCATTTCG GAGCCCTATT CCAATCTGGG TAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168913.1
CDS	78..5885
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWVS

Target ORF information:

RefSeq Version	XM_017313424.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313424.1

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGCA AGGAATCCCA CAGATGAGAA TGACTTGGAA 
ATGCAGAAGA GAGAACAGCT CGGAGGGTGG TTTCCTCACA CCCAAGGCCT TACCTGGGGA 
GACGCAGGTC AGGGGAGCGC TAATGGTGAG CAGCCACGTG CAGATGTCCA TGCTGAGCAC 
AATGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG AGTCATTTTC AGAACAGACA GAGCATCTAG AACAAGAATC TACCAATAAG 
CAGGAAGAGA CTGATCCTCT TAGTATTGCA GTCCCTTCAG TTATTTATCA GCAACAGCAT 
TCCTTAGGTG CTCATAACTC TCTGTTGCCC ATGGAGGAAG AAAGTACATC TGATCATACA 
CATGTTCAGC AGATCATGGA CAATGATGTA AATGAAGCAA ATTTGATACC TGACAAAAGA 
GACTTTCAGG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGAGCTCAG AATCTTCATC TGAAAAACAT 
GTTAAGGCAT TATCCACAGG CACAATCTGC TTCCCAGCAC TGACAGCCAA GTCACATTCT 
CCTAACCCAA GGCTGAACCA ACTTGATATT AACCTGGCTC ATGCAACAAC TGAGGGATCT 
GAACAGTCTT TTCAACAGCT TCGACCAGAA TTCTCTTCAC AGGAAAGCCA ACATGCTGAC 
CTACCCAGTA TTTATAGCAT TGAAGCAAGA GGTCCTTCCC AACGCATGGA AAACCAGAAC 
TATTCTGAAA TGTTACAAAA TAAGAAAAAA AGTCTCTCTC TCCAGCCCTC AACAGAGGAT 
TTGACTCCAG CTTGCAGTTC ATCTGATACT GCTCTGTTTG ATCAGTTACA CCTGCAGCAC 
AGCACTCCGT GTGCTTCTGT CTCTAGTGAG TGCTCAGTAA AACTGTTAGA AAGCAGAGAA 
GAAGTGCTGG GATTTGAAGA GCTGTCAAGA AGGGCAGTTA CAATGTCACA AAGACTCACT 
GAAGATGAAA ATGTAGTTCT ACCTATAAAC CCACATGTAG GAAGAGTGGA GAAGGAGGCT 
TCAGTTCAGG GGTCTAATCC ATTGAGTATT CAAAATGAAA AGCCAATTCA GAACTTCATT 
GAAACTGACA CTACAGAAGC TGTAGGAAAT GTATGTCAGC TAGCACAAGC AGAGCACATA 
TTAAAATCCT GTCCATTTCG GAGCCCTATT CCAATCTGGG TAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_017313424.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168913.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X16, mRNA.

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CloneID	OMu82855
Clone ID Related Accession (Same CDS sequence)	XM_017313424.1 , XM_017313424.2
Accession Version	XM_017313424.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017313424.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGCA AGGAATCCCA CAGATGAGAA TGACTTGGAA 
ATGCAGAAGA GAGAACAGCT CGGAGGGTGG TTTCCTCACA CCCAAGGCCT TACCTGGGGA 
GACGCAGGTC AGGGGAGCGC TAATGGTGAG CAGCCACGTG CAGATGTCCA TGCTGAGCAC 
AATGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG AGTCATTTTC AGAACAGACA GAGCATCTAG AACAAGAATC TACCAATAAG 
CAGGAAGAGA CTGATCCTCT TAGTATTGCA GTCCCTTCAG TTATTTATCA GCAACAGCAT 
TCCTTAGGTG CTCATAACTC TCTGTTGCCC ATGGAGGAAG AAAGTACATC TGATCATACA 
CATGTTCAGC AGATCATGGA CAATGATGTA AATGAAGCAA ATTTGATACC TGACAAAAGA 
GACTTTCAGG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGAGCTCAG AATCTTCATC TGAAAAACAT 
GTTAAGGCAT TATCCACAGG CACAATCTGC TTCCCAGCAC TGACAGCCAA GTCACATTCT 
CCTAACCCAA GGCTGAACCA ACTTGATATT AACCTGGCTC ATGCAACAAC TGAGGGATCT 
GAACAGTCTT TTCAACAGCT TCGACCAGAA TTCTCTTCAC AGGAAAGCCA ACATGCTGAC 
CTACCCAGTA TTTATAGCAT TGAAGCAAGA GGTCCTTCCC AACGCATGGA AAACCAGAAC 
TATTCTGAAA TGTTACAAAA TAAGAAAAAA AGTCTCTCTC TCCAGCCCTC AACAGAGGAT 
TTGACTCCAG CTTGCAGTTC ATCTGATACT GCTCTGTTTG ATCAGTTACA CCTGCAGCAC 
AGCACTCCGT GTGCTTCTGT CTCTAGTGAG TGCTCAGTAA AACTGTTAGA AAGCAGAGAA 
GAAGTGCTGG GATTTGAAGA GCTGTCAAGA AGGGCAGTTA CAATGTCACA AAGACTCACT 
GAAGATGAAA ATGTAGTTCT ACCTATAAAC CCACATGTAG GAAGAGTGGA GAAGGAGGCT 
TCAGTTCAGG GGTCTAATCC ATTGAGTATT CAAAATGAAA AGCCAATTCA GAACTTCATT 
GAAACTGACA CTACAGAAGC TGTAGGAAAT GTATGTCAGC TAGCACAAGC AGAGCACATA 
TTAAAATCCT GTCCATTTCG GAGCCCTATT CCAATCTGGG TAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168913.1
CDS	107..5914
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWVS

Target ORF information:

RefSeq Version	XM_017313424.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313424.2

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGCA AGGAATCCCA CAGATGAGAA TGACTTGGAA 
ATGCAGAAGA GAGAACAGCT CGGAGGGTGG TTTCCTCACA CCCAAGGCCT TACCTGGGGA 
GACGCAGGTC AGGGGAGCGC TAATGGTGAG CAGCCACGTG CAGATGTCCA TGCTGAGCAC 
AATGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG AGTCATTTTC AGAACAGACA GAGCATCTAG AACAAGAATC TACCAATAAG 
CAGGAAGAGA CTGATCCTCT TAGTATTGCA GTCCCTTCAG TTATTTATCA GCAACAGCAT 
TCCTTAGGTG CTCATAACTC TCTGTTGCCC ATGGAGGAAG AAAGTACATC TGATCATACA 
CATGTTCAGC AGATCATGGA CAATGATGTA AATGAAGCAA ATTTGATACC TGACAAAAGA 
GACTTTCAGG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGAGCTCAG AATCTTCATC TGAAAAACAT 
GTTAAGGCAT TATCCACAGG CACAATCTGC TTCCCAGCAC TGACAGCCAA GTCACATTCT 
CCTAACCCAA GGCTGAACCA ACTTGATATT AACCTGGCTC ATGCAACAAC TGAGGGATCT 
GAACAGTCTT TTCAACAGCT TCGACCAGAA TTCTCTTCAC AGGAAAGCCA ACATGCTGAC 
CTACCCAGTA TTTATAGCAT TGAAGCAAGA GGTCCTTCCC AACGCATGGA AAACCAGAAC 
TATTCTGAAA TGTTACAAAA TAAGAAAAAA AGTCTCTCTC TCCAGCCCTC AACAGAGGAT 
TTGACTCCAG CTTGCAGTTC ATCTGATACT GCTCTGTTTG ATCAGTTACA CCTGCAGCAC 
AGCACTCCGT GTGCTTCTGT CTCTAGTGAG TGCTCAGTAA AACTGTTAGA AAGCAGAGAA 
GAAGTGCTGG GATTTGAAGA GCTGTCAAGA AGGGCAGTTA CAATGTCACA AAGACTCACT 
GAAGATGAAA ATGTAGTTCT ACCTATAAAC CCACATGTAG GAAGAGTGGA GAAGGAGGCT 
TCAGTTCAGG GGTCTAATCC ATTGAGTATT CAAAATGAAA AGCCAATTCA GAACTTCATT 
GAAACTGACA CTACAGAAGC TGTAGGAAAT GTATGTCAGC TAGCACAAGC AGAGCACATA 
TTAAAATCCT GTCCATTTCG GAGCCCTATT CCAATCTGGG TAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_017313425.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168914.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X13, mRNA.

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CloneID	OMu82856
Clone ID Related Accession (Same CDS sequence)	XM_017313425.1
Accession Version	XM_017313425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGA TCCACTTGGC CCAAAACCAG GAGCGATTGA TGGAAGAACT CAAACAGCTG 
CAGAAAGAGG ACCTGGCCCG TAGGAGGCAG ACCGTGGCAC AGATGCCGCC ACAGATGCTG 
GAGTTGCCAT ACCGACGGAG TGAGATGAAG GAAGACAGGC AGCGAGAGCT GGAGTTTGCC 
TTTGAAGATA TGTACAATGC AGATAGGAAG GTGAAAGGAA ACCTGATTTT ACATCTTAAG 
CCAGAACCTT TGCCTACAAT CTCTGATCAG CTCCAAGATG AAGAACTGGA CCTTTCAATG 
GAACAAGAAA ATCAAGTGCC CTTGGCAGCA AAGATCCAAC AAATCCCCTC AAGAATTCTT 
TTTAAAAGAT TATTAAACAA GATTAGAAGT CAAAAATCTC TCTGGACAAT TAAATCTGTG 
TCTGAGGATG AAGGTGAGGT GACTTCGAGC ATCATTGAGA TTGAGAGTAA AGTGCCAAGC 
GTGGACTCGG GAGCCATTAT CACAGAGGAG AGAACAGCGG CGTCCTTTGA GCAGGAGCAA 
GTCACAGACA GTGATAGGCT GACGATAGAA TCTGGACCAC TGAGCAGTGA AGATAAGCCA 
TTGTACTACA AAGCGGGGAC TGGAAGGGAA CAAGCAATGG CAGTGTCTCC ACCTGCCACA 
GCTGTGGCTC AGAGTTCAGT CCTGCTTCAT CCTCAGGAAG AGGCAGTCAG AATTCGAATG 
TCATTAAGGC GCAAACAGAT AATGGAGATA GAAGAGCAGA AGCAGAAGCA GTTGGAATTA 
CTTGAACAAA TTGAGCAACA GAAGTTAAGG TTAGAAACTG ATTGTTTCCG GGCTCAACTG 
GAAGAGCAAA GAAAGCAAGC TGATCAGCCT GAGGTTTGCT GTGCTCCAAT GTCACATGCT 
ATGATTTCTG ATGAAGACTC TCACAGGCAG ATGATTCGTA ACTATCAACA TCAGCTTTTA 
CAACAAAACA GGTTACACAA GGAGACTGTT GAAACTGCCA GGAAACGATT ACTCGAATAC 
CAAACTGTAT TAAAAGAAAG ATCCCCATCC CTGTCAGCCT CAGCACTGGT ACCTGATTCT 
GTTGTGTCAG GGCCACCACA GCAATCCTAC AAGCCTGCTG CTGCTTCAGA CTCTTGGGAT 
CCAAGCCAGA GACTGAAACT AAGTCCTAGC AAATATCAAC CTGTGCAACC CTCACAGATC 
CCTGCATTAG AGCAGAGTCA TATTCAGGTA CCAAGACATG GTCACATTAC ACAGAGGCAA 
GGAAAAATGG CTGTATCAGA AATGTTAGGT AAGCAGCCCG TGGAGTCACA AGAACGTCAG 
TGGCAGTTCT CTCAGGTGGA AACACATCAG GGAGACTATG AGTTCGTCCT TAAAGATTCT 
CATTCACTTT CAAGGACTTT GTCTTATGTT AGGCCACAAA CATTACAAGA TGCTAGAGAA 
GTATCTAAAC CTCCTAGGGT AATAATTTGT CAAAGTCTAG ATTCCCAACA GATATCATCA 
GAGGATAGTG AAAATATATC TTCTAAGCCA AGTGAACCGT CTCCTTTTCT CCCACTGGTG 
CCTGAACGCC CTTTTACTAG TCTCCCAGTT AAATTCCATT CTGGAACAAT TCACAAACCC 
TTTACGACCA TAAACCAGTC TGTAATCAGT CAAATGCATG ATCAACCTTT GTCATCCTCA 
GAGACTATTA CAGCTCAGCA GGGTGACTTA AGATTTCTTC AAGAACAGTT AGAACTACAG 
AAGAAAGTTC TTCAGGCAAG ACAAGAGGCT CGGGAAAAGC TGCTTTTGTG CACACAGAAA 
GAACTGGGAC AGCAGACTGG CCTCCCAGTG TTCCTCCCTT CACCAGCTGG AAATATATTT 
TCTTCATTAC CCTCTGCCTC AGCTGAATCA GGAAACTTTC AGACATCTTC AACAAAAAGT 
GATGCCACTG TTTCCTCAGA CAATATGGAC AGGCTTTGGG ATTCTTCACA ACCCATCTCA 
TCACAGCAAA CTCATTTAGA ATTTCTCCAA GAGCAGTCCA GTGTTGAGAC GGATAACCTT 
CAGGCTAGGC GGGAAGCCCA GGAGGTCTTG TTTGCACATA CACAAAATAC GCTGGAAAAA 
ATTGTACGTT CTGAACAAGC TGGGTCTTCT TTGCCACATC AGGTAGCTCA GCAGTCATTT 
AGTTCACTAA CATTGGCTGA TACACAATCT AAAAAAATCC AGAAACAGCC TTTACCTGCA 
AACAAGAAAG GGCTACTCCC AAGCCAGTCT GAAGTCTCAA AAGCTCAGGA TGGATCTTCA 
GGTTTTCTAC AGCAGACCCT ACCTCTCCAG AACACTTTAA AGTTGCTTCA AGAGCAGCTG 
ACTAGACAGA GGAGTATGAT TCCGCCTAGA CGTGATGGTC AAGAGACATT ACTTTTATAT 
AAAGAAAGCT GTTCAGAAGA CAGTGAGGCT GGGCCAGTAG AATCACTCTC CTCCGTGGTT 
GTTCAGCATG CAGATGCTTC TCGTGCAGTT TCAGAAGTAC CTAAGCGGCT CCAGGACGTG 
TATTCATCTG AGGAAGAAAA TAGAGTTCTC TCCAGTCATT TGATCACCCA TGGCTTTCCA 
CAGCACAGCC TGCAAAGACA AGAACATTTT ACACCACTCC AGGAAGAGAC ACACATTCAG 
AGGCTTATAC TTGGTGCTAG AAAAAATAAT GAGGAATTTG CTCCCAAGCA AAATGAATTA 
GAAAAGGGGC TCTGCTCTCA ACAGACTGAT GCCTTGTCTT CTCCATCCCA AGTCACCGAC 
TGGGGAACAT CCCGGGGGTC TGTATCAGTC AGAAGTGATA GGACAGACCC CTTAAGGCAT 
TTTAAGATTC CAGCGTTTCG GGAAAGACTA GTTAGAGTTT CACAACATAC ATTCCCTCTC 
CAAGATAATT TGCAGGAACA CCAAGAATGG GTAGACACAG AAAAAGAGAG CTTTCAATCT 
AGTCCTCTAA CTCCAGAAAA TCCATCTTCT CAACAGACAG GTTTTTCGTC CTTCAAAGCC 
TCATTAAGAC TGCCATCGTG TGTGTCCTTG CCATCTGCTG ACTCTGGTAT CACACAGCAC 
CCTCTCTCAA CAGAGAGTGA CAGTAAAGTC AAGTCAAGCC ATCTTCAGAT ACCAGAACTG 
CAGCATAGAC TTTCGAAGAT ATCCCAGCTC ATCCCGCCTC AACAAGACAG TTTGAAGGCC 
CTTCAAGAAC AATTAGCTAC ACAAAGGGAA GCCATCATCC ACTCTAGACA AGAAGCTCAT 
GAAGAAACAC TAAGAGAATG GAAGGAAAAA ATATTCCCAG AGCAGGCAAG GAATCCCACA 
GATGAGAATG ACTTGGAAAT GCAGAAGAGA GAACAGCTCG GAGGGTGGTT TCCTCACACC 
CAAGGCCTTA CCTGGGGAGA CGCAGGTCAG GGGAGCGCTA ATGGTGAGCA GCCACGTGCA 
GATGTCCATG CTGAGCACAA TGGAAAAGCA GAGTTTTATC AAGACCGAGA CCCATTAAGG 
GTCTCAGTAA GCAGAGAACA AAGTTTCTTG GAGAGCCCAC TGGCACATGA TCCATTTGGT 
TGTCACCAAC CACCTGCCCA AGAGAACAGC AAAAGCCATG ACGACAATGC TGAAGCAGTT 
AAAGTCAAGA AATCTGATGT TGAGGATCAT GCAGTATTGA GTCATGCTGT CTCAAAAGAA 
GAAGCGTGTA CAAACCTGGG TCCACTTGGA AAGCCAGACG ATGAGGCTGA AACACAAGAG 
ATTTCTCAGG AGCCATTGTC TTCAGTAACT GTTTCTACTG GAAGCTTCTT AAGTTATGAA 
ATCACAGATT TGAGCCTCAC AGATCCAGAG TCATTTTCAG AACAGACAGA GCATCTAGAA 
CAAGAATCTA CCAATAAGCA GGAAGAGACT GATCCTCTTA GTATTGCAGT CCCTTCAGTT 
ATTTATCAGC AACAGCATTC CTTAGGTGCT CATAACTCTC TGTTGCCCAT GGAGGAAGAA 
AGTACATCTG ATCATACACA TGTTCAGCAG ATCATGGACA ATGATGTAAA TGAAGCAAAT 
TTGATACCTG ACAAAAGAGA CTTTCAGGTT CCAGCTGTGG ACCTTGACTT TCGAGAATTG 
GAACACATAT TTCCACACTT GCATCGTCAG CTCTTTAAAC CCTTAGAACC ACATCTAGAT 
TTTGACTTAT CATCCCCTGG GACATCTCAA GAAGACAGCG ACTTTTACCA GAGCTCAGAA 
TCTTCATCTG AAAAACATGT TAAGGCATTA TCCACAGGCA CAATCTGCTT CCCAGCACTG 
ACAGCCAAGT CACATTCTCC TAACCCAAGG CTGAACCAAC TTGATATTAA CCTGGCTCAT 
GCAACAACTG AGGGATCTGA ACAGTCTTTT CAACAGCTTC GACCAGAATT CTCTTCACAG 
GAAAGCCAAC ATGCTGACCT ACCCAGTATT TATAGCATTG AAGCAAGAGG TCCTTCCCAA 
CGCATGGAAA ACCAGAACTA TTCTGAAATG TTACAAAATA AGAAAAAAAG TCTCTCTCTC 
CAGCCCTCAA CAGAGGATTT GACTCCAGCT TGCAGTTCAT CTGATACTGC TCTGTTTGAT 
CAGTTACACC TGCAGCACAG CACTCCGTGT GCTTCTGTCT CTAGTGAGTG CTCAGTAAAA 
CTGTTAGAAA GCAGAGAAGA AGTGCTGGGA TTTGAAGAGC TGTCAAGAAG GGCAGTTACA 
ATGTCACAAA GACTCACTGA AGATGAAAAT GTAGTTCTAC CTATAAACCC ACATGTAGGA 
AGAGTGGAGA AGGAGGCTTC AGTTCAGGGG TCTAATCCAT TGAGTATTCA AAATGAAAAG 
CCAATTCAGA ACTTCATTGA AACTGACACT ACAGAAGCTG TAGGAAATGT ATGTCAGCTA 
GCACAAGCAG AGCACATATT AAAATCCTGT CCATTTCGGA GCCCTATTCC AATCTGGGTA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168914.1
CDS	236..5161
Translation	MKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWVS

Target ORF information:

RefSeq Version	XM_017313425.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313425.1

ORF Insert Sequence:

ATGAAAAAGA TCCACTTGGC CCAAAACCAG GAGCGATTGA TGGAAGAACT CAAACAGCTG 
CAGAAAGAGG ACCTGGCCCG TAGGAGGCAG ACCGTGGCAC AGATGCCGCC ACAGATGCTG 
GAGTTGCCAT ACCGACGGAG TGAGATGAAG GAAGACAGGC AGCGAGAGCT GGAGTTTGCC 
TTTGAAGATA TGTACAATGC AGATAGGAAG GTGAAAGGAA ACCTGATTTT ACATCTTAAG 
CCAGAACCTT TGCCTACAAT CTCTGATCAG CTCCAAGATG AAGAACTGGA CCTTTCAATG 
GAACAAGAAA ATCAAGTGCC CTTGGCAGCA AAGATCCAAC AAATCCCCTC AAGAATTCTT 
TTTAAAAGAT TATTAAACAA GATTAGAAGT CAAAAATCTC TCTGGACAAT TAAATCTGTG 
TCTGAGGATG AAGGTGAGGT GACTTCGAGC ATCATTGAGA TTGAGAGTAA AGTGCCAAGC 
GTGGACTCGG GAGCCATTAT CACAGAGGAG AGAACAGCGG CGTCCTTTGA GCAGGAGCAA 
GTCACAGACA GTGATAGGCT GACGATAGAA TCTGGACCAC TGAGCAGTGA AGATAAGCCA 
TTGTACTACA AAGCGGGGAC TGGAAGGGAA CAAGCAATGG CAGTGTCTCC ACCTGCCACA 
GCTGTGGCTC AGAGTTCAGT CCTGCTTCAT CCTCAGGAAG AGGCAGTCAG AATTCGAATG 
TCATTAAGGC GCAAACAGAT AATGGAGATA GAAGAGCAGA AGCAGAAGCA GTTGGAATTA 
CTTGAACAAA TTGAGCAACA GAAGTTAAGG TTAGAAACTG ATTGTTTCCG GGCTCAACTG 
GAAGAGCAAA GAAAGCAAGC TGATCAGCCT GAGGTTTGCT GTGCTCCAAT GTCACATGCT 
ATGATTTCTG ATGAAGACTC TCACAGGCAG ATGATTCGTA ACTATCAACA TCAGCTTTTA 
CAACAAAACA GGTTACACAA GGAGACTGTT GAAACTGCCA GGAAACGATT ACTCGAATAC 
CAAACTGTAT TAAAAGAAAG ATCCCCATCC CTGTCAGCCT CAGCACTGGT ACCTGATTCT 
GTTGTGTCAG GGCCACCACA GCAATCCTAC AAGCCTGCTG CTGCTTCAGA CTCTTGGGAT 
CCAAGCCAGA GACTGAAACT AAGTCCTAGC AAATATCAAC CTGTGCAACC CTCACAGATC 
CCTGCATTAG AGCAGAGTCA TATTCAGGTA CCAAGACATG GTCACATTAC ACAGAGGCAA 
GGAAAAATGG CTGTATCAGA AATGTTAGGT AAGCAGCCCG TGGAGTCACA AGAACGTCAG 
TGGCAGTTCT CTCAGGTGGA AACACATCAG GGAGACTATG AGTTCGTCCT TAAAGATTCT 
CATTCACTTT CAAGGACTTT GTCTTATGTT AGGCCACAAA CATTACAAGA TGCTAGAGAA 
GTATCTAAAC CTCCTAGGGT AATAATTTGT CAAAGTCTAG ATTCCCAACA GATATCATCA 
GAGGATAGTG AAAATATATC TTCTAAGCCA AGTGAACCGT CTCCTTTTCT CCCACTGGTG 
CCTGAACGCC CTTTTACTAG TCTCCCAGTT AAATTCCATT CTGGAACAAT TCACAAACCC 
TTTACGACCA TAAACCAGTC TGTAATCAGT CAAATGCATG ATCAACCTTT GTCATCCTCA 
GAGACTATTA CAGCTCAGCA GGGTGACTTA AGATTTCTTC AAGAACAGTT AGAACTACAG 
AAGAAAGTTC TTCAGGCAAG ACAAGAGGCT CGGGAAAAGC TGCTTTTGTG CACACAGAAA 
GAACTGGGAC AGCAGACTGG CCTCCCAGTG TTCCTCCCTT CACCAGCTGG AAATATATTT 
TCTTCATTAC CCTCTGCCTC AGCTGAATCA GGAAACTTTC AGACATCTTC AACAAAAAGT 
GATGCCACTG TTTCCTCAGA CAATATGGAC AGGCTTTGGG ATTCTTCACA ACCCATCTCA 
TCACAGCAAA CTCATTTAGA ATTTCTCCAA GAGCAGTCCA GTGTTGAGAC GGATAACCTT 
CAGGCTAGGC GGGAAGCCCA GGAGGTCTTG TTTGCACATA CACAAAATAC GCTGGAAAAA 
ATTGTACGTT CTGAACAAGC TGGGTCTTCT TTGCCACATC AGGTAGCTCA GCAGTCATTT 
AGTTCACTAA CATTGGCTGA TACACAATCT AAAAAAATCC AGAAACAGCC TTTACCTGCA 
AACAAGAAAG GGCTACTCCC AAGCCAGTCT GAAGTCTCAA AAGCTCAGGA TGGATCTTCA 
GGTTTTCTAC AGCAGACCCT ACCTCTCCAG AACACTTTAA AGTTGCTTCA AGAGCAGCTG 
ACTAGACAGA GGAGTATGAT TCCGCCTAGA CGTGATGGTC AAGAGACATT ACTTTTATAT 
AAAGAAAGCT GTTCAGAAGA CAGTGAGGCT GGGCCAGTAG AATCACTCTC CTCCGTGGTT 
GTTCAGCATG CAGATGCTTC TCGTGCAGTT TCAGAAGTAC CTAAGCGGCT CCAGGACGTG 
TATTCATCTG AGGAAGAAAA TAGAGTTCTC TCCAGTCATT TGATCACCCA TGGCTTTCCA 
CAGCACAGCC TGCAAAGACA AGAACATTTT ACACCACTCC AGGAAGAGAC ACACATTCAG 
AGGCTTATAC TTGGTGCTAG AAAAAATAAT GAGGAATTTG CTCCCAAGCA AAATGAATTA 
GAAAAGGGGC TCTGCTCTCA ACAGACTGAT GCCTTGTCTT CTCCATCCCA AGTCACCGAC 
TGGGGAACAT CCCGGGGGTC TGTATCAGTC AGAAGTGATA GGACAGACCC CTTAAGGCAT 
TTTAAGATTC CAGCGTTTCG GGAAAGACTA GTTAGAGTTT CACAACATAC ATTCCCTCTC 
CAAGATAATT TGCAGGAACA CCAAGAATGG GTAGACACAG AAAAAGAGAG CTTTCAATCT 
AGTCCTCTAA CTCCAGAAAA TCCATCTTCT CAACAGACAG GTTTTTCGTC CTTCAAAGCC 
TCATTAAGAC TGCCATCGTG TGTGTCCTTG CCATCTGCTG ACTCTGGTAT CACACAGCAC 
CCTCTCTCAA CAGAGAGTGA CAGTAAAGTC AAGTCAAGCC ATCTTCAGAT ACCAGAACTG 
CAGCATAGAC TTTCGAAGAT ATCCCAGCTC ATCCCGCCTC AACAAGACAG TTTGAAGGCC 
CTTCAAGAAC AATTAGCTAC ACAAAGGGAA GCCATCATCC ACTCTAGACA AGAAGCTCAT 
GAAGAAACAC TAAGAGAATG GAAGGAAAAA ATATTCCCAG AGCAGGCAAG GAATCCCACA 
GATGAGAATG ACTTGGAAAT GCAGAAGAGA GAACAGCTCG GAGGGTGGTT TCCTCACACC 
CAAGGCCTTA CCTGGGGAGA CGCAGGTCAG GGGAGCGCTA ATGGTGAGCA GCCACGTGCA 
GATGTCCATG CTGAGCACAA TGGAAAAGCA GAGTTTTATC AAGACCGAGA CCCATTAAGG 
GTCTCAGTAA GCAGAGAACA AAGTTTCTTG GAGAGCCCAC TGGCACATGA TCCATTTGGT 
TGTCACCAAC CACCTGCCCA AGAGAACAGC AAAAGCCATG ACGACAATGC TGAAGCAGTT 
AAAGTCAAGA AATCTGATGT TGAGGATCAT GCAGTATTGA GTCATGCTGT CTCAAAAGAA 
GAAGCGTGTA CAAACCTGGG TCCACTTGGA AAGCCAGACG ATGAGGCTGA AACACAAGAG 
ATTTCTCAGG AGCCATTGTC TTCAGTAACT GTTTCTACTG GAAGCTTCTT AAGTTATGAA 
ATCACAGATT TGAGCCTCAC AGATCCAGAG TCATTTTCAG AACAGACAGA GCATCTAGAA 
CAAGAATCTA CCAATAAGCA GGAAGAGACT GATCCTCTTA GTATTGCAGT CCCTTCAGTT 
ATTTATCAGC AACAGCATTC CTTAGGTGCT CATAACTCTC TGTTGCCCAT GGAGGAAGAA 
AGTACATCTG ATCATACACA TGTTCAGCAG ATCATGGACA ATGATGTAAA TGAAGCAAAT 
TTGATACCTG ACAAAAGAGA CTTTCAGGTT CCAGCTGTGG ACCTTGACTT TCGAGAATTG 
GAACACATAT TTCCACACTT GCATCGTCAG CTCTTTAAAC CCTTAGAACC ACATCTAGAT 
TTTGACTTAT CATCCCCTGG GACATCTCAA GAAGACAGCG ACTTTTACCA GAGCTCAGAA 
TCTTCATCTG AAAAACATGT TAAGGCATTA TCCACAGGCA CAATCTGCTT CCCAGCACTG 
ACAGCCAAGT CACATTCTCC TAACCCAAGG CTGAACCAAC TTGATATTAA CCTGGCTCAT 
GCAACAACTG AGGGATCTGA ACAGTCTTTT CAACAGCTTC GACCAGAATT CTCTTCACAG 
GAAAGCCAAC ATGCTGACCT ACCCAGTATT TATAGCATTG AAGCAAGAGG TCCTTCCCAA 
CGCATGGAAA ACCAGAACTA TTCTGAAATG TTACAAAATA AGAAAAAAAG TCTCTCTCTC 
CAGCCCTCAA CAGAGGATTT GACTCCAGCT TGCAGTTCAT CTGATACTGC TCTGTTTGAT 
CAGTTACACC TGCAGCACAG CACTCCGTGT GCTTCTGTCT CTAGTGAGTG CTCAGTAAAA 
CTGTTAGAAA GCAGAGAAGA AGTGCTGGGA TTTGAAGAGC TGTCAAGAAG GGCAGTTACA 
ATGTCACAAA GACTCACTGA AGATGAAAAT GTAGTTCTAC CTATAAACCC ACATGTAGGA 
AGAGTGGAGA AGGAGGCTTC AGTTCAGGGG TCTAATCCAT TGAGTATTCA AAATGAAAAG 
CCAATTCAGA ACTTCATTGA AACTGACACT ACAGAAGCTG TAGGAAATGT ATGTCAGCTA 
GCACAAGCAG AGCACATATT AAAATCCTGT CCATTTCGGA GCCCTATTCC AATCTGGGTA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_006510409.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510472.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X3, mRNA.

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CloneID	OMu42262
Clone ID Related Accession (Same CDS sequence)	XM_006510409.1 , XM_006510409.2
Accession Version	XM_006510409.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG AGTCATTTTC AGAACAGACA GAGCATCTAG AACAAGAATC TACCAATAAG 
CAGGAAGAGA CTGATCCTCT TAGTATTGCA GTCCCTTCAG TTATTTATCA GCAACAGCAT 
TCCTTAGGTG CTCATAACTC TCTGTTGCCC ATGGAGGAAG AAAGTACATC TGATCATACA 
CATGTTCAGC AGATCATGGA CAATGATGTA AATGAAGCAA ATTTGATACC TGACAAAAGA 
GACTTTCAGG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGCAGAGCT CAGAATCTTC ATCTGAAAAA 
CATGTTAAGG CATTATCCAC AGGCACAATC TGCTTCCCAG CACTGACAGC CAAGTCACAT 
TCTCCTAACC CAAGGCTGAA CCAACTTGAT ATTAACCTGG CTCATGCAAC AACTGAGGGA 
TCTGAACAGT CTTTTCAACA GCTTCGACCA GAATTCTCTT CACAGGAAAG CCAACATGCT 
GACCTACCCA GTATTTATAG CATTGAAGCA AGAGGTCCTT CCCAACGCAT GGAAAACCAG 
AACTATTCTG AAATGTTACA AAATAAGAAA AAAAGTCTCT CTCTCCAGCC CTCAACAGAG 
GATTTGACTC CAGCTTGCAG TTCATCTGAT ACTGCTCTGT TTGATCAGTT ACACCTGCAG 
CACAGCACTC CGTGTGCTTC TGTCTCTAGT GAGTGCTCAG TAAAACTGTT AGAAAGCAGA 
GAAGAAGTGC TGGGATTTGA AGAGCTGTCA AGAAGGGCAG TTACAATGTC ACAAAGACTC 
ACTGAAGATG AAAATGTAGT TCTACCTATA AACCCACATG TAGGAAGAGT GGAGAAGGAG 
GCTTCAGTTC AGGGGTCTAA TCCATTGAGT ATTCAAAATG AAAAGCCAAT TCAGAACTTC 
ATTGAAACTG ACACTACAGA AGCTGTAGGA AATGTATGTC AGCTAGCACA AGCAGAGCAC 
ATATTAAAAT CCTGTCCATT TCGGAGCCCT ATTCCAATCT GGGAAACGGA TACTGGCTAT 
GGTATAATGG AAGAACCAGA TCTCACTCTA GTGAGTAATA GTGACATCAG CATCACTGAA 
ACAGATCTTG CAAACTTAAC CCTTGAAGAC AGAGAAGACA ATGAAGCACA GTTTTTTCAG 
GCAGGTGTCG TTCTGCCTAC ATCTTCAATG GAAACCTCTG TTTGTGGAGC TGTATCAGAG 
CCTTATGTGG ACCAACCCAC AGTGGCACCT TCAGCTACAT CAGGTAGTTT ACAAGAAGCA 
TTTATGACAA GACAGACACT TACAGAACGA TCTTACCAGA GGCAGAGAGA GATCTGGAAT 
AAAACTCGAC TCCCTCAAAC CAAAGTATCC AAAGAGAAGC TACCTACAGG TTGCACAGGC 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510472.1
CDS	107..7492
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_006510409.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510409.2

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG AGTCATTTTC AGAACAGACA GAGCATCTAG AACAAGAATC TACCAATAAG 
CAGGAAGAGA CTGATCCTCT TAGTATTGCA GTCCCTTCAG TTATTTATCA GCAACAGCAT 
TCCTTAGGTG CTCATAACTC TCTGTTGCCC ATGGAGGAAG AAAGTACATC TGATCATACA 
CATGTTCAGC AGATCATGGA CAATGATGTA AATGAAGCAA ATTTGATACC TGACAAAAGA 
GACTTTCAGG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGCAGAGCT CAGAATCTTC ATCTGAAAAA 
CATGTTAAGG CATTATCCAC AGGCACAATC TGCTTCCCAG CACTGACAGC CAAGTCACAT 
TCTCCTAACC CAAGGCTGAA CCAACTTGAT ATTAACCTGG CTCATGCAAC AACTGAGGGA 
TCTGAACAGT CTTTTCAACA GCTTCGACCA GAATTCTCTT CACAGGAAAG CCAACATGCT 
GACCTACCCA GTATTTATAG CATTGAAGCA AGAGGTCCTT CCCAACGCAT GGAAAACCAG 
AACTATTCTG AAATGTTACA AAATAAGAAA AAAAGTCTCT CTCTCCAGCC CTCAACAGAG 
GATTTGACTC CAGCTTGCAG TTCATCTGAT ACTGCTCTGT TTGATCAGTT ACACCTGCAG 
CACAGCACTC CGTGTGCTTC TGTCTCTAGT GAGTGCTCAG TAAAACTGTT AGAAAGCAGA 
GAAGAAGTGC TGGGATTTGA AGAGCTGTCA AGAAGGGCAG TTACAATGTC ACAAAGACTC 
ACTGAAGATG AAAATGTAGT TCTACCTATA AACCCACATG TAGGAAGAGT GGAGAAGGAG 
GCTTCAGTTC AGGGGTCTAA TCCATTGAGT ATTCAAAATG AAAAGCCAAT TCAGAACTTC 
ATTGAAACTG ACACTACAGA AGCTGTAGGA AATGTATGTC AGCTAGCACA AGCAGAGCAC 
ATATTAAAAT CCTGTCCATT TCGGAGCCCT ATTCCAATCT GGGAAACGGA TACTGGCTAT 
GGTATAATGG AAGAACCAGA TCTCACTCTA GTGAGTAATA GTGACATCAG CATCACTGAA 
ACAGATCTTG CAAACTTAAC CCTTGAAGAC AGAGAAGACA ATGAAGCACA GTTTTTTCAG 
GCAGGTGTCG TTCTGCCTAC ATCTTCAATG GAAACCTCTG TTTGTGGAGC TGTATCAGAG 
CCTTATGTGG ACCAACCCAC AGTGGCACCT TCAGCTACAT CAGGTAGTTT ACAAGAAGCA 
TTTATGACAA GACAGACACT TACAGAACGA TCTTACCAGA GGCAGAGAGA GATCTGGAAT 
AAAACTCGAC TCCCTCAAAC CAAAGTATCC AAAGAGAAGC TACCTACAGG TTGCACAGGC 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_006510411.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510474.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X6, mRNA.

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CloneID	OMu42264
Clone ID Related Accession (Same CDS sequence)	XM_006510411.1 , XM_006510411.2
Accession Version	XM_006510411.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510411.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GAAAAGCAGA GTTTTATCAA 
GACCGAGACC CATTAAGGGT CTCAGTAAGC AGAGAACAAA GTTTCTTGGA GAGCCCACTG 
GCACATGATC CATTTGGTTG TCACCAACCA CCTGCCCAAG AGAACAGCAA AAGCCATGAC 
GACAATGCTG AAGCAGTTAA AGTCAAGAAA TCTGATGTTG AGGATCATGC AGTATTGAGT 
CATGCTGTCT CAAAAGAAGA AGCGTGTACA AACCTGGGTC CACTTGGAAA GCCAGACGAT 
GAGGCTGAAA CACAAGAGAT TTCTCAGGAG CCATTGTCTT CAGTAACTGT TTCTACTGGA 
AGCTTCTTAA GTTATGAAAT CACAGATTTG AGCCTCACAG ATCCAGAGTC ATTTTCAGAA 
CAGACAGAGC ATCTAGAACA AGAATCTACC AATAAGCAGG AAGAGACTGA TCCTCTTAGT 
ATTGCAGTCC CTTCAGTTAT TTATCAGCAA CAGCATTCCT TAGGTGCTCA TAACTCTCTG 
TTGCCCATGG AGGAAGAAAG TACATCTGAT CATACACATG TTCAGCAGAT CATGGACAAT 
GATGTAAATG AAGCAAATTT GATACCTGAC AAAAGAGACT TTCAGGTTCC AGCTGTGGAC 
CTTGACTTTC GAGAATTGGA ACACATATTT CCACACTTGC ATCGTCAGCT CTTTAAACCC 
TTAGAACCAC ATCTAGATTT TGACTTATCA TCCCCTGGGA CATCTCAAGA AGACAGCGAC 
TTTTACCAGC AGAGCTCAGA ATCTTCATCT GAAAAACATG TTAAGGCATT ATCCACAGGC 
ACAATCTGCT TCCCAGCACT GACAGCCAAG TCACATTCTC CTAACCCAAG GCTGAACCAA 
CTTGATATTA ACCTGGCTCA TGCAACAACT GAGGGATCTG AACAGTCTTT TCAACAGCTT 
CGACCAGAAT TCTCTTCACA GGAAAGCCAA CATGCTGACC TACCCAGTAT TTATAGCATT 
GAAGCAAGAG GTCCTTCCCA ACGCATGGAA AACCAGAACT ATTCTGAAAT GTTACAAAAT 
AAGAAAAAAA GTCTCTCTCT CCAGCCCTCA ACAGAGGATT TGACTCCAGC TTGCAGTTCA 
TCTGATACTG CTCTGTTTGA TCAGTTACAC CTGCAGCACA GCACTCCGTG TGCTTCTGTC 
TCTAGTGAGT GCTCAGTAAA ACTGTTAGAA AGCAGAGAAG AAGTGCTGGG ATTTGAAGAG 
CTGTCAAGAA GGGCAGTTAC AATGTCACAA AGACTCACTG AAGATGAAAA TGTAGTTCTA 
CCTATAAACC CACATGTAGG AAGAGTGGAG AAGGAGGCTT CAGTTCAGGG GTCTAATCCA 
TTGAGTATTC AAAATGAAAA GCCAATTCAG AACTTCATTG AAACTGACAC TACAGAAGCT 
GTAGGAAATG TATGTCAGCT AGCACAAGCA GAGCACATAT TAAAATCCTG TCCATTTCGG 
AGCCCTATTC CAATCTGGGA AACGGATACT GGCTATGGTA TAATGGAAGA ACCAGATCTC 
ACTCTAGTGA GTAATAGTGA CATCAGCATC ACTGAAACAG ATCTTGCAAA CTTAACCCTT 
GAAGACAGAG AAGACAATGA AGCACAGTTT TTTCAGGCAG GTGTCGTTCT GCCTACATCT 
TCAATGGAAA CCTCTGTTTG TGGAGCTGTA TCAGAGCCTT ATGTGGACCA ACCCACAGTG 
GCACCTTCAG CTACATCAGG TAGTTTACAA GAAGCATTTA TGACAAGACA GACACTTACA 
GAACGATCTT ACCAGAGGCA GAGAGAGATC TGGAATAAAA CTCGACTCCC TCAAACCAAA 
GTATCCAAAG AGAAGCTACC TACAGGTTGC ACAGGCAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510474.1
CDS	107..7348
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_006510411.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510411.2

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GAAAAGCAGA GTTTTATCAA 
GACCGAGACC CATTAAGGGT CTCAGTAAGC AGAGAACAAA GTTTCTTGGA GAGCCCACTG 
GCACATGATC CATTTGGTTG TCACCAACCA CCTGCCCAAG AGAACAGCAA AAGCCATGAC 
GACAATGCTG AAGCAGTTAA AGTCAAGAAA TCTGATGTTG AGGATCATGC AGTATTGAGT 
CATGCTGTCT CAAAAGAAGA AGCGTGTACA AACCTGGGTC CACTTGGAAA GCCAGACGAT 
GAGGCTGAAA CACAAGAGAT TTCTCAGGAG CCATTGTCTT CAGTAACTGT TTCTACTGGA 
AGCTTCTTAA GTTATGAAAT CACAGATTTG AGCCTCACAG ATCCAGAGTC ATTTTCAGAA 
CAGACAGAGC ATCTAGAACA AGAATCTACC AATAAGCAGG AAGAGACTGA TCCTCTTAGT 
ATTGCAGTCC CTTCAGTTAT TTATCAGCAA CAGCATTCCT TAGGTGCTCA TAACTCTCTG 
TTGCCCATGG AGGAAGAAAG TACATCTGAT CATACACATG TTCAGCAGAT CATGGACAAT 
GATGTAAATG AAGCAAATTT GATACCTGAC AAAAGAGACT TTCAGGTTCC AGCTGTGGAC 
CTTGACTTTC GAGAATTGGA ACACATATTT CCACACTTGC ATCGTCAGCT CTTTAAACCC 
TTAGAACCAC ATCTAGATTT TGACTTATCA TCCCCTGGGA CATCTCAAGA AGACAGCGAC 
TTTTACCAGC AGAGCTCAGA ATCTTCATCT GAAAAACATG TTAAGGCATT ATCCACAGGC 
ACAATCTGCT TCCCAGCACT GACAGCCAAG TCACATTCTC CTAACCCAAG GCTGAACCAA 
CTTGATATTA ACCTGGCTCA TGCAACAACT GAGGGATCTG AACAGTCTTT TCAACAGCTT 
CGACCAGAAT TCTCTTCACA GGAAAGCCAA CATGCTGACC TACCCAGTAT TTATAGCATT 
GAAGCAAGAG GTCCTTCCCA ACGCATGGAA AACCAGAACT ATTCTGAAAT GTTACAAAAT 
AAGAAAAAAA GTCTCTCTCT CCAGCCCTCA ACAGAGGATT TGACTCCAGC TTGCAGTTCA 
TCTGATACTG CTCTGTTTGA TCAGTTACAC CTGCAGCACA GCACTCCGTG TGCTTCTGTC 
TCTAGTGAGT GCTCAGTAAA ACTGTTAGAA AGCAGAGAAG AAGTGCTGGG ATTTGAAGAG 
CTGTCAAGAA GGGCAGTTAC AATGTCACAA AGACTCACTG AAGATGAAAA TGTAGTTCTA 
CCTATAAACC CACATGTAGG AAGAGTGGAG AAGGAGGCTT CAGTTCAGGG GTCTAATCCA 
TTGAGTATTC AAAATGAAAA GCCAATTCAG AACTTCATTG AAACTGACAC TACAGAAGCT 
GTAGGAAATG TATGTCAGCT AGCACAAGCA GAGCACATAT TAAAATCCTG TCCATTTCGG 
AGCCCTATTC CAATCTGGGA AACGGATACT GGCTATGGTA TAATGGAAGA ACCAGATCTC 
ACTCTAGTGA GTAATAGTGA CATCAGCATC ACTGAAACAG ATCTTGCAAA CTTAACCCTT 
GAAGACAGAG AAGACAATGA AGCACAGTTT TTTCAGGCAG GTGTCGTTCT GCCTACATCT 
TCAATGGAAA CCTCTGTTTG TGGAGCTGTA TCAGAGCCTT ATGTGGACCA ACCCACAGTG 
GCACCTTCAG CTACATCAGG TAGTTTACAA GAAGCATTTA TGACAAGACA GACACTTACA 
GAACGATCTT ACCAGAGGCA GAGAGAGATC TGGAATAAAA CTCGACTCCC TCAAACCAAA 
GTATCCAAAG AGAAGCTACC TACAGGTTGC ACAGGCAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_006510414.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510477.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X15, mRNA.

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CloneID	OMu42267
Clone ID Related Accession (Same CDS sequence)	XM_006510414.1 , XM_006510414.2
Accession Version	XM_006510414.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510414.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGCA AGGAATCCCA CAGATGAGAA TGACTTGGAA 
ATGCAGAAGA GAGAACAGCT CGGAGGGTGG TTTCCTCACA CCCAAGGCCT TACCTGGGGA 
GACGCAGGTC AGGGGAGCGC TAATGGTGAG CAGCCACGTG CAGATGTCCA TGCTGAGCAC 
AATGGTGAGA GTCTGGCCAA AGAACTGAGT GGTAGAGCCT CAAAGCCACC TGTCTCCAAA 
GTTAAATGTG TCTTGGACTT AAACCAGCAT GAGCTTAGTA CTATACAGGA AGTAGAGTCA 
CCAGCAAGTG GCAGGATTTC CATGCCAGGA AAAGCAGAGT TTTATCAAGA CCGAGACCCA 
TTAAGGGTCT CAGTAAGCAG AGAACAAAGT TTCTTGGAGA GCCCACTGGC ACATGATCCA 
TTTGGTTGTC ACCAACCACC TGCCCAAGAG AACAGCAAAA GCCATGACGA CAATGCTGAA 
GCAGTTAAAG TCAAGAAATC TGATGTTGAG GATCATGCAG TATTGAGTCA TGCTGTCTCA 
AAAGAAGAAG CGTGTACAAA CCTGGGTCCA CTTGGAAAGC CAGACGATGA GGCTGAAACA 
CAAGAGATTT CTCAGGAGCC ATTGTCTTCA GTAACTGTTT CTACTGGAAG CTTCTTAAGT 
TATGAAATCA CAGATTTGAG CCTCACAGAT CCAGTTCCAG CTGTGGACCT TGACTTTCGA 
GAATTGGAAC ACATATTTCC ACACTTGCAT CGTCAGCTCT TTAAACCCTT AGAACCACAT 
CTAGATTTTG ACTTATCATC CCCTGGGACA TCTCAAGAAG ACAGCGACTT TTACCAGAGC 
TCAGAATCTT CATCTGAAAA ACATGTTAAG GCATTATCCA CAGGCACAAT CTGCTTCCCA 
GCACTGACAG CCAAGTCACA TTCTCCTAAC CCAAGGCTGA ACCAACTTGA TATTAACCTG 
GCTCATGCAA CAACTGAGGG ATCTGAACAG TCTTTTCAAC AGCTTCGACC AGAATTCTCT 
TCACAGGAAA GCCAACATGC TGACCTACCC AGTATTTATA GCATTGAAGC AAGAGGTCCT 
TCCCAACGCA TGGAAAACCA GAACTATTCT GAAATGTTAC AAAATAAGAA AAAAAGTCTC 
TCTCTCCAGC CCTCAACAGA GGATTTGACT CCAGCTTGCA GTTCATCTGA TACTGCTCTG 
TTTGATCAGT TACACCTGCA GCACAGCACT CCGTGTGCTT CTGTCTCTAG TGAGTGCTCA 
GTAAAACTGT TAGAAAGCAG AGAAGAAGTG CTGGGATTTG AAGAGCTGTC AAGAAGGGCA 
GTTACAATGT CACAAAGACT CACTGAAGAT GAAAATGTAG TTCTACCTAT AAACCCACAT 
GTAGGAAGAG TGGAGAAGGA GGCTTCAGTT CAGGGGTCTA ATCCATTGAG TATTCAAAAT 
GAAAAGCCAA TTCAGAACTT CATTGAAACT GACACTACAG AAGCTGTAGG AAATGTATGT 
CAGCTAGCAC AAGCAGAGCA CATATTAAAA TCCTGTCCAT TTCGGAGCCC TATTCCAATC 
TGGGAAACGG ATACTGGCTA TGGTATAATG GAAGAACCAG ATCTCACTCT AGTGAGTAAT 
AGTGACATCA GCATCACTGA AACAGATCTT GCAAACTTAA CCCTTGAAGA CAGAGAAGAC 
AATGAAGCAC AGTTTTTTCA GGCAGGTGTC GTTCTGCCTA CATCTTCAAT GGAAACCTCT 
GTTTGTGGAG CTGTATCAGA GCCTTATGTG GACCAACCCA CAGTGGCACC TTCAGCTACA 
TCAGGTAGTT TACAAGAAGC ATTTATGACA AGACAGACAC TTACAGAACG ATCTTACCAG 
AGGCAGAGAG AGATCTGGAA TAAAACTCGA CTCCCTCAAA CCAAAGTATC CAAAGAGAAG 
CTACCTACAG GTTGCACAGG CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510477.1
CDS	107..6193
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_006510414.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510414.2

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGCA AGGAATCCCA CAGATGAGAA TGACTTGGAA 
ATGCAGAAGA GAGAACAGCT CGGAGGGTGG TTTCCTCACA CCCAAGGCCT TACCTGGGGA 
GACGCAGGTC AGGGGAGCGC TAATGGTGAG CAGCCACGTG CAGATGTCCA TGCTGAGCAC 
AATGGTGAGA GTCTGGCCAA AGAACTGAGT GGTAGAGCCT CAAAGCCACC TGTCTCCAAA 
GTTAAATGTG TCTTGGACTT AAACCAGCAT GAGCTTAGTA CTATACAGGA AGTAGAGTCA 
CCAGCAAGTG GCAGGATTTC CATGCCAGGA AAAGCAGAGT TTTATCAAGA CCGAGACCCA 
TTAAGGGTCT CAGTAAGCAG AGAACAAAGT TTCTTGGAGA GCCCACTGGC ACATGATCCA 
TTTGGTTGTC ACCAACCACC TGCCCAAGAG AACAGCAAAA GCCATGACGA CAATGCTGAA 
GCAGTTAAAG TCAAGAAATC TGATGTTGAG GATCATGCAG TATTGAGTCA TGCTGTCTCA 
AAAGAAGAAG CGTGTACAAA CCTGGGTCCA CTTGGAAAGC CAGACGATGA GGCTGAAACA 
CAAGAGATTT CTCAGGAGCC ATTGTCTTCA GTAACTGTTT CTACTGGAAG CTTCTTAAGT 
TATGAAATCA CAGATTTGAG CCTCACAGAT CCAGTTCCAG CTGTGGACCT TGACTTTCGA 
GAATTGGAAC ACATATTTCC ACACTTGCAT CGTCAGCTCT TTAAACCCTT AGAACCACAT 
CTAGATTTTG ACTTATCATC CCCTGGGACA TCTCAAGAAG ACAGCGACTT TTACCAGAGC 
TCAGAATCTT CATCTGAAAA ACATGTTAAG GCATTATCCA CAGGCACAAT CTGCTTCCCA 
GCACTGACAG CCAAGTCACA TTCTCCTAAC CCAAGGCTGA ACCAACTTGA TATTAACCTG 
GCTCATGCAA CAACTGAGGG ATCTGAACAG TCTTTTCAAC AGCTTCGACC AGAATTCTCT 
TCACAGGAAA GCCAACATGC TGACCTACCC AGTATTTATA GCATTGAAGC AAGAGGTCCT 
TCCCAACGCA TGGAAAACCA GAACTATTCT GAAATGTTAC AAAATAAGAA AAAAAGTCTC 
TCTCTCCAGC CCTCAACAGA GGATTTGACT CCAGCTTGCA GTTCATCTGA TACTGCTCTG 
TTTGATCAGT TACACCTGCA GCACAGCACT CCGTGTGCTT CTGTCTCTAG TGAGTGCTCA 
GTAAAACTGT TAGAAAGCAG AGAAGAAGTG CTGGGATTTG AAGAGCTGTC AAGAAGGGCA 
GTTACAATGT CACAAAGACT CACTGAAGAT GAAAATGTAG TTCTACCTAT AAACCCACAT 
GTAGGAAGAG TGGAGAAGGA GGCTTCAGTT CAGGGGTCTA ATCCATTGAG TATTCAAAAT 
GAAAAGCCAA TTCAGAACTT CATTGAAACT GACACTACAG AAGCTGTAGG AAATGTATGT 
CAGCTAGCAC AAGCAGAGCA CATATTAAAA TCCTGTCCAT TTCGGAGCCC TATTCCAATC 
TGGGAAACGG ATACTGGCTA TGGTATAATG GAAGAACCAG ATCTCACTCT AGTGAGTAAT 
AGTGACATCA GCATCACTGA AACAGATCTT GCAAACTTAA CCCTTGAAGA CAGAGAAGAC 
AATGAAGCAC AGTTTTTTCA GGCAGGTGTC GTTCTGCCTA CATCTTCAAT GGAAACCTCT 
GTTTGTGGAG CTGTATCAGA GCCTTATGTG GACCAACCCA CAGTGGCACC TTCAGCTACA 
TCAGGTAGTT TACAAGAAGC ATTTATGACA AGACAGACAC TTACAGAACG ATCTTACCAG 
AGGCAGAGAG AGATCTGGAA TAAAACTCGA CTCCCTCAAA CCAAAGTATC CAAAGAGAAG 
CTACCTACAG GTTGCACAGG CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_011242547.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240849.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X2, mRNA.

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CloneID	OMu74558
Clone ID Related Accession (Same CDS sequence)	XM_011242547.1 , XM_011242547.2
Accession Version	XM_011242547.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242547.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGTAAAC AAGGAAAAGC AGAGTTTTAT CAAGACCGAG ACCCATTAAG GGTCTCAGTA 
AGCAGAGAAC AAAGTTTCTT GGAGAGCCCA CTGGCACATG ATCCATTTGG TTGTCACCAA 
CCACCTGCCC AAGAGAACAG CAAAAGCCAT GACGACAATG CTGAAGCAGT TAAAGTCAAG 
AAATCTGATG TTGAGGATCA TGCAGTATTG AGTCATGCTG TCTCAAAAGA AGAAGCGTGT 
ACAAACCTGG GTCCACTTGG AAAGCCAGAC GATGAGGCTG AAACACAAGA GATTTCTCAG 
GAGCCATTGT CTTCAGTAAC TGTTTCTACT GGAAGCTTCT TAAGTTATGA AATCACAGAT 
TTGAGCCTCA CAGATCCAGA GTCATTTTCA GAACAGACAG AGCATCTAGA ACAAGAATCT 
ACCAATAAGC AGGAAGAGAC TGATCCTCTT AGTATTGCAG TCCCTTCAGT TATTTATCAG 
CAACAGCATT CCTTAGGTGC TCATAACTCT CTGTTGCCCA TGGAGGAAGA AAGTACATCT 
GATCATACAC ATGTTCAGCA GATCATGGAC AATGATGTAA ATGAAGCAAA TTTGATACCT 
GACAAAAGAG ACTTTCAGGT TCCAGCTGTG GACCTTGACT TTCGAGAATT GGAACACATA 
TTTCCACACT TGCATCGTCA GCTCTTTAAA CCCTTAGAAC CACATCTAGA TTTTGACTTA 
TCATCCCCTG GGACATCTCA AGAAGACAGC GACTTTTACC AGAGCTCAGA ATCTTCATCT 
GAAAAACATG TTAAGGCATT ATCCACAGGC ACAATCTGCT TCCCAGCACT GACAGCCAAG 
TCACATTCTC CTAACCCAAG GCTGAACCAA CTTGATATTA ACCTGGCTCA TGCAACAACT 
GAGGGATCTG AACAGTCTTT TCAACAGCTT CGACCAGAAT TCTCTTCACA GGAAAGCCAA 
CATGCTGACC TACCCAGTAT TTATAGCATT GAAGCAAGAG GTCCTTCCCA ACGCATGGAA 
AACCAGAACT ATTCTGAAAT GTTACAAAAT AAGAAAAAAA GTCTCTCTCT CCAGCCCTCA 
ACAGAGGATT TGACTCCAGC TTGCAGTTCA TCTGATACTG CTCTGTTTGA TCAGTTACAC 
CTGCAGCACA GCACTCCGTG TGCTTCTGTC TCTAGTGAGT GCTCAGTAAA ACTGTTAGAA 
AGCAGAGAAG AAGTGCTGGG ATTTGAAGAG CTGTCAAGAA GGGCAGTTAC AATGTCACAA 
AGACTCACTG AAGATGAAAA TGTAGTTCTA CCTATAAACC CACATGTAGG AAGAGTGGAG 
AAGGAGGCTT CAGTTCAGGG GTCTAATCCA TTGAGTATTC AAAATGAAAA GCCAATTCAG 
AACTTCATTG AAACTGACAC TACAGAAGCT GTAGGAAATG TATGTCAGCT AGCACAAGCA 
GAGCACATAT TAAAATCCTG TCCATTTCGG AGCCCTATTC CAATCTGGGA AACGGATACT 
GGCTATGGTA TAATGGAAGA ACCAGATCTC ACTCTAGTGA GTAATAGTGA CATCAGCATC 
ACTGAAACAG ATCTTGCAAA CTTAACCCTT GAAGACAGAG AAGACAATGA AGCACAGTTT 
TTTCAGGCAG GTGTCGTTCT GCCTACATCT TCAATGGAAA CCTCTGTTTG TGGAGCTGTA 
TCAGAGCCTT ATGTGGACCA ACCCACAGTG GCACCTTCAG CTACATCAGG TAGTTTACAA 
GAAGCATTTA TGACAAGACA GACACTTACA GAACGATCTT ACCAGAGGCA GAGAGAGATC 
TGGAATAAAA CTCGACTCCC TCAAACCAAA GTATCCAAAG AGAAGCTACC TACAGGTTGC 
ACAGGCAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240849.1
CDS	107..7498
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_011242547.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242547.2

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGTAAAC AAGGAAAAGC AGAGTTTTAT CAAGACCGAG ACCCATTAAG GGTCTCAGTA 
AGCAGAGAAC AAAGTTTCTT GGAGAGCCCA CTGGCACATG ATCCATTTGG TTGTCACCAA 
CCACCTGCCC AAGAGAACAG CAAAAGCCAT GACGACAATG CTGAAGCAGT TAAAGTCAAG 
AAATCTGATG TTGAGGATCA TGCAGTATTG AGTCATGCTG TCTCAAAAGA AGAAGCGTGT 
ACAAACCTGG GTCCACTTGG AAAGCCAGAC GATGAGGCTG AAACACAAGA GATTTCTCAG 
GAGCCATTGT CTTCAGTAAC TGTTTCTACT GGAAGCTTCT TAAGTTATGA AATCACAGAT 
TTGAGCCTCA CAGATCCAGA GTCATTTTCA GAACAGACAG AGCATCTAGA ACAAGAATCT 
ACCAATAAGC AGGAAGAGAC TGATCCTCTT AGTATTGCAG TCCCTTCAGT TATTTATCAG 
CAACAGCATT CCTTAGGTGC TCATAACTCT CTGTTGCCCA TGGAGGAAGA AAGTACATCT 
GATCATACAC ATGTTCAGCA GATCATGGAC AATGATGTAA ATGAAGCAAA TTTGATACCT 
GACAAAAGAG ACTTTCAGGT TCCAGCTGTG GACCTTGACT TTCGAGAATT GGAACACATA 
TTTCCACACT TGCATCGTCA GCTCTTTAAA CCCTTAGAAC CACATCTAGA TTTTGACTTA 
TCATCCCCTG GGACATCTCA AGAAGACAGC GACTTTTACC AGAGCTCAGA ATCTTCATCT 
GAAAAACATG TTAAGGCATT ATCCACAGGC ACAATCTGCT TCCCAGCACT GACAGCCAAG 
TCACATTCTC CTAACCCAAG GCTGAACCAA CTTGATATTA ACCTGGCTCA TGCAACAACT 
GAGGGATCTG AACAGTCTTT TCAACAGCTT CGACCAGAAT TCTCTTCACA GGAAAGCCAA 
CATGCTGACC TACCCAGTAT TTATAGCATT GAAGCAAGAG GTCCTTCCCA ACGCATGGAA 
AACCAGAACT ATTCTGAAAT GTTACAAAAT AAGAAAAAAA GTCTCTCTCT CCAGCCCTCA 
ACAGAGGATT TGACTCCAGC TTGCAGTTCA TCTGATACTG CTCTGTTTGA TCAGTTACAC 
CTGCAGCACA GCACTCCGTG TGCTTCTGTC TCTAGTGAGT GCTCAGTAAA ACTGTTAGAA 
AGCAGAGAAG AAGTGCTGGG ATTTGAAGAG CTGTCAAGAA GGGCAGTTAC AATGTCACAA 
AGACTCACTG AAGATGAAAA TGTAGTTCTA CCTATAAACC CACATGTAGG AAGAGTGGAG 
AAGGAGGCTT CAGTTCAGGG GTCTAATCCA TTGAGTATTC AAAATGAAAA GCCAATTCAG 
AACTTCATTG AAACTGACAC TACAGAAGCT GTAGGAAATG TATGTCAGCT AGCACAAGCA 
GAGCACATAT TAAAATCCTG TCCATTTCGG AGCCCTATTC CAATCTGGGA AACGGATACT 
GGCTATGGTA TAATGGAAGA ACCAGATCTC ACTCTAGTGA GTAATAGTGA CATCAGCATC 
ACTGAAACAG ATCTTGCAAA CTTAACCCTT GAAGACAGAG AAGACAATGA AGCACAGTTT 
TTTCAGGCAG GTGTCGTTCT GCCTACATCT TCAATGGAAA CCTCTGTTTG TGGAGCTGTA 
TCAGAGCCTT ATGTGGACCA ACCCACAGTG GCACCTTCAG CTACATCAGG TAGTTTACAA 
GAAGCATTTA TGACAAGACA GACACTTACA GAACGATCTT ACCAGAGGCA GAGAGAGATC 
TGGAATAAAA CTCGACTCCC TCAAACCAAA GTATCCAAAG AGAAGCTACC TACAGGTTGC 
ACAGGCAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_011242548.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240850.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X5, mRNA.

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CloneID	OMu74559
Clone ID Related Accession (Same CDS sequence)	XM_011242548.2 , XM_011242548.3
Accession Version	XM_011242548.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242548.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTCC AGATGCTCAT TTGGCTGCTG AAGAAGAAGC TAGACGAGTG 
GAAAGACTAC GGAAACAGGC GGCACAGGAG AGAGTGAAAC AGTTTGAAAG GGCACATGTG 
CGGGGATCCC AAGCCATGAA AAAGATCCAC TTGGCCCAAA ACCAGGAGCG ATTGATGGAA 
GAACTCAAAC AGCTGCAGAA AGAGGACCTG GCCCGTAGGA GGCAGACCGT GGCACAGATG 
CCGCCACAGA TGCTGGAGTT GCCATACCGA CGGAGTGAGA TGAAGGAAGA CAGGCAGCGA 
GAGCTGGAGT TTGCCTTTGA AGATATGTAC AATGCAGATA GGAAGGTGAA AGGAAACCTG 
ATTTTACATC TTAAGCCAGA ACCTTTGCCT ACAATCTCTG ATCAGCTCCA AGATGAAGAA 
CTGGACCTTT CAATGGAACA AGAAAATCAA GTGCCCTTGG CAGCAAAGAT CCAACAAATC 
CCCTCAAGAA TTCTTTTTAA AAGATTATTA AACAAGATTA GAAGTCAAAA ATCTCTCTGG 
ACAATTAAAT CTGTGTCTGA GGATGAAGGT GAGGTGACTT CGAGCATCAT TGAGATTGAG 
AGTAAAGTGC CAAGCGTGGA CTCGGGAGCC ATTATCACAG AGGAGAGAAC AGCGGCGTCC 
TTTGAGCAGG AGCAAGTCAC AGACAGTGAT AGGCTGACGA TAGAATCTGG ACCACTGAGC 
AGTGAAGATA AGCCATTGTA CTACAAAGCG GGGACTGGAA GGGAACAAGC AATGGCAGTG 
TCTCCACCTG CCACAGCTGT GGCTCAGAGT TCAGTCCTGC TTCATCCTCA GGAAGAGGCA 
GTCAGAATTC GAATGTCATT AAGGCGCAAA CAGATAATGG AGATAGAAGA GCAGAAGCAG 
AAGCAGTTGG AATTACTTGA ACAAATTGAG CAACAGAAGT TAAGGTTAGA AACTGATTGT 
TTCCGGGCTC AACTGGAAGA GCAAAGAAAG CAAGCTGATC AGCCTGAGGT TTGCTGTGCT 
CCAATGTCAC ATGCTATGAT TTCTGATGAA GACTCTCACA GGCAGATGAT TCGTAACTAT 
CAACATCAGC TTTTACAACA AAACAGGTTA CACAAGGAGA CTGTTGAAAC TGCCAGGAAA 
CGATTACTCG AATACCAAAC TGTATTAAAA GAAAGATCCC CATCCCTGTC AGCCTCAGCA 
CTGGTACCTG ATTCTGTTGT GTCAGGGCCA CCACAGCAAT CCTACAAGCC TGCTGCTGCT 
TCAGACTCTT GGGATCCAAG CCAGAGACTG AAACTAAGTC CTAGCAAATA TCAACCTGTG 
CAACCCTCAC AGATCCCTGC ATTAGAGCAG AGTCATATTC AGGTACCAAG ACATGGTCAC 
ATTACACAGA GGCAAGGAAA AATGGCTGTA TCAGAAATGT TAGGTAAGCA GCCCGTGGAG 
TCACAAGAAC GTCAGTGGCA GTTCTCTCAG GTGGAAACAC ATCAGGGAGA CTATGAGTTC 
GTCCTTAAAG ATTCTCATTC ACTTTCAAGG ACTTTGTCTT ATGTTAGGCC ACAAACATTA 
CAAGATGCTA GAGAAGTATC TAAACCTCCT AGGGTAATAA TTTGTCAAAG TCTAGATTCC 
CAACAGATAT CATCAGAGGA TAGTGAAAAT ATATCTTCTA AGCCAAGTGA ACCGTCTCCT 
TTTCTCCCAC TGGTGCCTGA ACGCCCTTTT ACTAGTCTCC CAGTTAAATT CCATTCTGGA 
ACAATTCACA AACCCTTTAC GACCATAAAC CAGTCTGTAA TCAGTCAAAT GCATGATCAA 
CCTTTGTCAT CCTCAGAGAC TATTACAGCT CAGCAGGGTG ACTTAAGATT TCTTCAAGAA 
CAGTTAGAAC TACAGAAGAA AGTTCTTCAG GCAAGACAAG AGGCTCGGGA AAAGCTGCTT 
TTGTGCACAC AGAAAGAACT GGGACAGCAG ACTGGCCTCC CAGTGTTCCT CCCTTCACCA 
GCTGGAAATA TATTTTCTTC ATTACCCTCT GCCTCAGCTG AATCAGGAAA CTTTCAGACA 
TCTTCAACAA AAAGTGATGC CACTGTTTCC TCAGACAATA TGGACAGGCT TTGGGATTCT 
TCACAACCCA TCTCATCACA GCAAACTCAT TTAGAATTTC TCCAAGAGCA GTCCAGTGTT 
GAGACGGATA ACCTTCAGGC TAGGCGGGAA GCCCAGGAGG TCTTGTTTGC ACATACACAA 
AATACGCTGG AAAAAATTGT ACGTTCTGAA CAAGCTGGGT CTTCTTTGCC ACATCAGGTA 
GCTCAGCAGT CATTTAGTTC ACTAACATTG GCTGATACAC AATCTAAAAA AATCCAGAAA 
CAGCCTTTAC CTGCAAACAA GAAAGGGCTA CTCCCAAGCC AGTCTGAAGT CTCAAAAGCT 
CAGGATGGAT CTTCAGGTTT TCTACAGCAG ACCCTACCTC TCCAGAACAC TTTAAAGTTG 
CTTCAAGAGC AGCTGACTAG ACAGAGGAGT ATGATTCCGC CTAGACGTGA TGGTCAAGAG 
ACATTACTTT TATATAAAGA AAGCTGTTCA GAAGACAGTG AGGCTGGGCC AGTAGAATCA 
CTCTCCTCCG TGGTTGTTCA GCATGCAGAT GCTTCTCGTG CAGTTTCAGA AGTACCTAAG 
CGGCTCCAGG ACGTGTATTC ATCTGAGGAA GAAAATAGAG TTCTCTCCAG TCATTTGATC 
ACCCATGGCT TTCCACAGCA CAGCCTGCAA AGACAAGAAC ATTTTACACC ACTCCAGGAA 
GAGACACACA TTCAGAGGCT TATACTTGGT GCTAGAAAAA ATAATGAGGA ATTTGCTCCC 
AAGCAAAATG AATTAGAAAA GGGGCTCTGC TCTCAACAGA CTGATGCCTT GTCTTCTCCA 
TCCCAAGTCA CCGACTGGGG AACATCCCGG GGGTCTGTAT CAGTCAGAAG TGATAGGACA 
GACCCCTTAA GGCATTTTAA GATTCCAGCG TTTCGGGAAA GACTAGTTAG AGTTTCACAA 
CATACATTCC CTCTCCAAGA TAATTTGCAG GAACACCAAG AATGGGTAGA CACAGAAAAA 
GAGAGCTTTC AATCTAGTCC TCTAACTCCA GAAAATCCAT CTTCTCAACA GACAGGTTTT 
TCGTCCTTCA AAGCCTCATT AAGACTGCCA TCGTGTGTGT CCTTGCCATC TGCTGACTCT 
GGTATCACAC AGCACCCTCT CTCAACAGAG AGTGACAGTA AAGTCAAGTC AAGCCATCTT 
CAGATACCAG AACTGCAGCA TAGACTTTCG AAGATATCCC AGCTCATCCC GCCTCAACAA 
GACAGTTTGA AGGCCCTTCA AGAACAATTA GCTACACAAA GGGAAGCCAT CATCCACTCT 
AGACAAGAAG CTCATGAAGA AACACTAAGA GAATGGAAGG AAAAAATATT CCCAGAGCAG 
GTTGGTCCAT TTTCTCCCCT GATACCACAA CATTCACTTG CTTCATTCCC TGTTTCTGAT 
ACTGAAAGAG CACAAGAACT GTGTTCAACC AACAGTGACA CCATATCTTC AGGTTATCCT 
GAGATGCTGG AGTTGCCTGA CAGGACATTG GGTTTATCAC ATACTGCTTT ACCTCAGCAG 
AATAATCTGA CTGCACATCC AGAACACCTT CATGCACAAA CAAATTTTTT TCACTCTACT 
GAGAAAGCCC AAGAAGGGTT GGTGTTTCCC AGACCATGTC AATTTGAAGA GATGTCTGCT 
GAGCATTTTA TCCAGCCTCA ACATGATGAC TTAAAGGCAC TTCAACAGCA GTTAGATATG 
CAAAGGGAAG CCATTAGATC TGGCCAGGAA ATGCAAGAGA AAATGCTTTT GCAAAGGTTA 
AATAAATTGG AGCAAAGGAT ATCTTCCAAA CAAATTAGCT CCTCTCTATT TTCATCCCAG 
GTAGCACTGC CCATTGCCAA TTCTGACGGG ACCTTACAGT CTTTCCCAAC TAAAAGTAAC 
GAGACTGAGC TGCTTGGGTC CCAAGATGAG TATCTGAGCT TTTCACAGCC TCGTCTGCCT 
CTGCAGAATA ACATGACAGA GCAGTTGGAC TTAGAAAAGG TATTTCATAA AGAACTGCTT 
TTACACAAAC AAAAGAGCCA AAACAAAAGT GAATCTTCTG AGCATTCCCT CCCTCCTTTG 
TTTCTGTCCA AGGAAATAGA GCATCCATTC ATTTCACTAC CTTTTGCAGA AAGTAAATCT 
AAAAGCATTT GTGAGTTGTA TTTATCTGAC AAGAAGCATG CAGCTCCTAA TGATGCTGTG 
ATCCCAAGAT TGCAGGACAG GCTTTTAAGC TGTTCCCAAC CTGTCTTAAC TCAGCAAGAT 
AACATGAGTC TTCAGAAGCA GCTGAATCTA CAAAGAGAAA CTCTACACTC TAGGCAAAAA 
GCCCAAGAAG AATTGCTTGT GCAGAGACAG ACATCCTTAC AGCAGCAAAT CCAAAGACAC 
AGAGAGACTT TGAAAAATTT CTTTAATGTC AGTCAGGCAA GGAATCCCAC AGATGAGAAT 
GACTTGGAAA TGCAGAAGAG AGAACAGCTC GGAGGGTGGT TTCCTCACAC CCAAGGCCTT 
ACCTGGGGAG ACGCAGGTCA GGGGAGCGCT AATGGTGAGC AGCCACGTGC AGATGTCCAT 
GCTGAGCACA ATGGTGAGAG TCTGGCCAAA GAACTGAGTG GTAGAGCCTC AAAGCCACCT 
GTCTCCAAAG TTAAATGTGT CTTGGACTTA AACCAGCATG AGCTTAGTAC TATACAGGAA 
GTAGAGTCAC CAGCAAGTGG CAGGATTTCC ATGCCAGGTA AACAAGGAAA AGCAGAGTTT 
TATCAAGACC GAGACCCATT AAGGGTCTCA GTAAGCAGAG AACAAAGTTT CTTGGAGAGC 
CCACTGGCAC ATGATCCATT TGGTTGTCAC CAACCACCTG CCCAAGAGAA CAGCAAAAGC 
CATGACGACA ATGCTGAAGC AGTTAAAGTC AAGAAATCTG ATGTTGAGGA TCATGCAGTA 
TTGAGTCATG CTGTCTCAAA AGAAGAAGCG TGTACAAACC TGGGTCCACT TGGAAAGCCA 
GACGATGAGG CTGAAACACA AGAGATTTCT CAGGAGCCAT TGTCTTCAGT AACTGTTTCT 
ACTGGAAGCT TCTTAAGTTA TGAAATCACA GATTTGAGCC TCACAGATCC AGAGTCATTT 
TCAGAACAGA CAGAGCATCT AGAACAAGAA TCTACCAATA AGCAGGAAGA GACTGATCCT 
CTTAGTATTG CAGTCCCTTC AGTTATTTAT CAGCAACAGC ATTCCTTAGG TGCTCATAAC 
TCTCTGTTGC CCATGGAGGA AGAAAGTACA TCTGATCATA CACATGTTCA GCAGATCATG 
GACAATGATG TAAATGAAGC AAATTTGATA CCTGACAAAA GAGACTTTCA GGTTCCAGCT 
GTGGACCTTG ACTTTCGAGA ATTGGAACAC ATATTTCCAC ACTTGCATCG TCAGCTCTTT 
AAACCCTTAG AACCACATCT AGATTTTGAC TTATCATCCC CTGGGACATC TCAAGAAGAC 
AGCGACTTTT ACCAGCAGAG CTCAGAATCT TCATCTGAAA AACATGTTAA GGCATTATCC 
ACAGGCACAA TCTGCTTCCC AGCACTGACA GCCAAGTCAC ATTCTCCTAA CCCAAGGCTG 
AACCAACTTG ATATTAACCT GGCTCATGCA ACAACTGAGG GATCTGAACA GTCTTTTCAA 
CAGCTTCGAC CAGAATTCTC TTCACAGGAA AGCCAACATG CTGACCTACC CAGTATTTAT 
AGCATTGAAG CAAGAGGTCC TTCCCAACGC ATGGAAAACC AGAACTATTC TGAAATGTTA 
CAAAATAAGA AAAAAAGTCT CTCTCTCCAG CCCTCAACAG AGGATTTGAC TCCAGCTTGC 
AGTTCATCTG ATACTGCTCT GTTTGATCAG TTACACCTGC AGCACAGCAC TCCGTGTGCT 
TCTGTCTCTA GTGAGTGCTC AGTAAAACTG TTAGAAAGCA GAGAAGAAGT GCTGGGATTT 
GAAGAGCTGT CAAGAAGGGC AGTTACAATG TCACAAAGAC TCACTGAAGA TGAAAATGTA 
GTTCTACCTA TAAACCCACA TGTAGGAAGA GTGGAGAAGG AGGCTTCAGT TCAGGGGTCT 
AATCCATTGA GTATTCAAAA TGAAAAGCCA ATTCAGAACT TCATTGAAAC TGACACTACA 
GAAGCTGTAG GAAATGTATG TCAGCTAGCA CAAGCAGAGC ACATATTAAA ATCCTGTCCA 
TTTCGGAGCC CTATTCCAAT CTGGGAAACG GATACTGGCT ATGGTATAAT GGAAGAACCA 
GATCTCACTC TAGTGAGTAA TAGTGACATC AGCATCACTG AAACAGATCT TGCAAACTTA 
ACCCTTGAAG ACAGAGAAGA CAATGAAGCA CAGTTTTTTC AGGCAGGTGT CGTTCTGCCT 
ACATCTTCAA TGGAAACCTC TGTTTGTGGA GCTGTATCAG AGCCTTATGT GGACCAACCC 
ACAGTGGCAC CTTCAGCTAC ATCAGGTAGT TTACAAGAAG CATTTATGAC AAGACAGACA 
CTTACAGAAC GATCTTACCA GAGGCAGAGA GAGATCTGGA ATAAAACTCG ACTCCCTCAA 
ACCAAAGTAT CCAAAGAGAA GCTACCTACA GGTTGCACAG GCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240850.1
CDS	76..7443
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_011242548.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242548.3

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTCC AGATGCTCAT TTGGCTGCTG AAGAAGAAGC TAGACGAGTG 
GAAAGACTAC GGAAACAGGC GGCACAGGAG AGAGTGAAAC AGTTTGAAAG GGCACATGTG 
CGGGGATCCC AAGCCATGAA AAAGATCCAC TTGGCCCAAA ACCAGGAGCG ATTGATGGAA 
GAACTCAAAC AGCTGCAGAA AGAGGACCTG GCCCGTAGGA GGCAGACCGT GGCACAGATG 
CCGCCACAGA TGCTGGAGTT GCCATACCGA CGGAGTGAGA TGAAGGAAGA CAGGCAGCGA 
GAGCTGGAGT TTGCCTTTGA AGATATGTAC AATGCAGATA GGAAGGTGAA AGGAAACCTG 
ATTTTACATC TTAAGCCAGA ACCTTTGCCT ACAATCTCTG ATCAGCTCCA AGATGAAGAA 
CTGGACCTTT CAATGGAACA AGAAAATCAA GTGCCCTTGG CAGCAAAGAT CCAACAAATC 
CCCTCAAGAA TTCTTTTTAA AAGATTATTA AACAAGATTA GAAGTCAAAA ATCTCTCTGG 
ACAATTAAAT CTGTGTCTGA GGATGAAGGT GAGGTGACTT CGAGCATCAT TGAGATTGAG 
AGTAAAGTGC CAAGCGTGGA CTCGGGAGCC ATTATCACAG AGGAGAGAAC AGCGGCGTCC 
TTTGAGCAGG AGCAAGTCAC AGACAGTGAT AGGCTGACGA TAGAATCTGG ACCACTGAGC 
AGTGAAGATA AGCCATTGTA CTACAAAGCG GGGACTGGAA GGGAACAAGC AATGGCAGTG 
TCTCCACCTG CCACAGCTGT GGCTCAGAGT TCAGTCCTGC TTCATCCTCA GGAAGAGGCA 
GTCAGAATTC GAATGTCATT AAGGCGCAAA CAGATAATGG AGATAGAAGA GCAGAAGCAG 
AAGCAGTTGG AATTACTTGA ACAAATTGAG CAACAGAAGT TAAGGTTAGA AACTGATTGT 
TTCCGGGCTC AACTGGAAGA GCAAAGAAAG CAAGCTGATC AGCCTGAGGT TTGCTGTGCT 
CCAATGTCAC ATGCTATGAT TTCTGATGAA GACTCTCACA GGCAGATGAT TCGTAACTAT 
CAACATCAGC TTTTACAACA AAACAGGTTA CACAAGGAGA CTGTTGAAAC TGCCAGGAAA 
CGATTACTCG AATACCAAAC TGTATTAAAA GAAAGATCCC CATCCCTGTC AGCCTCAGCA 
CTGGTACCTG ATTCTGTTGT GTCAGGGCCA CCACAGCAAT CCTACAAGCC TGCTGCTGCT 
TCAGACTCTT GGGATCCAAG CCAGAGACTG AAACTAAGTC CTAGCAAATA TCAACCTGTG 
CAACCCTCAC AGATCCCTGC ATTAGAGCAG AGTCATATTC AGGTACCAAG ACATGGTCAC 
ATTACACAGA GGCAAGGAAA AATGGCTGTA TCAGAAATGT TAGGTAAGCA GCCCGTGGAG 
TCACAAGAAC GTCAGTGGCA GTTCTCTCAG GTGGAAACAC ATCAGGGAGA CTATGAGTTC 
GTCCTTAAAG ATTCTCATTC ACTTTCAAGG ACTTTGTCTT ATGTTAGGCC ACAAACATTA 
CAAGATGCTA GAGAAGTATC TAAACCTCCT AGGGTAATAA TTTGTCAAAG TCTAGATTCC 
CAACAGATAT CATCAGAGGA TAGTGAAAAT ATATCTTCTA AGCCAAGTGA ACCGTCTCCT 
TTTCTCCCAC TGGTGCCTGA ACGCCCTTTT ACTAGTCTCC CAGTTAAATT CCATTCTGGA 
ACAATTCACA AACCCTTTAC GACCATAAAC CAGTCTGTAA TCAGTCAAAT GCATGATCAA 
CCTTTGTCAT CCTCAGAGAC TATTACAGCT CAGCAGGGTG ACTTAAGATT TCTTCAAGAA 
CAGTTAGAAC TACAGAAGAA AGTTCTTCAG GCAAGACAAG AGGCTCGGGA AAAGCTGCTT 
TTGTGCACAC AGAAAGAACT GGGACAGCAG ACTGGCCTCC CAGTGTTCCT CCCTTCACCA 
GCTGGAAATA TATTTTCTTC ATTACCCTCT GCCTCAGCTG AATCAGGAAA CTTTCAGACA 
TCTTCAACAA AAAGTGATGC CACTGTTTCC TCAGACAATA TGGACAGGCT TTGGGATTCT 
TCACAACCCA TCTCATCACA GCAAACTCAT TTAGAATTTC TCCAAGAGCA GTCCAGTGTT 
GAGACGGATA ACCTTCAGGC TAGGCGGGAA GCCCAGGAGG TCTTGTTTGC ACATACACAA 
AATACGCTGG AAAAAATTGT ACGTTCTGAA CAAGCTGGGT CTTCTTTGCC ACATCAGGTA 
GCTCAGCAGT CATTTAGTTC ACTAACATTG GCTGATACAC AATCTAAAAA AATCCAGAAA 
CAGCCTTTAC CTGCAAACAA GAAAGGGCTA CTCCCAAGCC AGTCTGAAGT CTCAAAAGCT 
CAGGATGGAT CTTCAGGTTT TCTACAGCAG ACCCTACCTC TCCAGAACAC TTTAAAGTTG 
CTTCAAGAGC AGCTGACTAG ACAGAGGAGT ATGATTCCGC CTAGACGTGA TGGTCAAGAG 
ACATTACTTT TATATAAAGA AAGCTGTTCA GAAGACAGTG AGGCTGGGCC AGTAGAATCA 
CTCTCCTCCG TGGTTGTTCA GCATGCAGAT GCTTCTCGTG CAGTTTCAGA AGTACCTAAG 
CGGCTCCAGG ACGTGTATTC ATCTGAGGAA GAAAATAGAG TTCTCTCCAG TCATTTGATC 
ACCCATGGCT TTCCACAGCA CAGCCTGCAA AGACAAGAAC ATTTTACACC ACTCCAGGAA 
GAGACACACA TTCAGAGGCT TATACTTGGT GCTAGAAAAA ATAATGAGGA ATTTGCTCCC 
AAGCAAAATG AATTAGAAAA GGGGCTCTGC TCTCAACAGA CTGATGCCTT GTCTTCTCCA 
TCCCAAGTCA CCGACTGGGG AACATCCCGG GGGTCTGTAT CAGTCAGAAG TGATAGGACA 
GACCCCTTAA GGCATTTTAA GATTCCAGCG TTTCGGGAAA GACTAGTTAG AGTTTCACAA 
CATACATTCC CTCTCCAAGA TAATTTGCAG GAACACCAAG AATGGGTAGA CACAGAAAAA 
GAGAGCTTTC AATCTAGTCC TCTAACTCCA GAAAATCCAT CTTCTCAACA GACAGGTTTT 
TCGTCCTTCA AAGCCTCATT AAGACTGCCA TCGTGTGTGT CCTTGCCATC TGCTGACTCT 
GGTATCACAC AGCACCCTCT CTCAACAGAG AGTGACAGTA AAGTCAAGTC AAGCCATCTT 
CAGATACCAG AACTGCAGCA TAGACTTTCG AAGATATCCC AGCTCATCCC GCCTCAACAA 
GACAGTTTGA AGGCCCTTCA AGAACAATTA GCTACACAAA GGGAAGCCAT CATCCACTCT 
AGACAAGAAG CTCATGAAGA AACACTAAGA GAATGGAAGG AAAAAATATT CCCAGAGCAG 
GTTGGTCCAT TTTCTCCCCT GATACCACAA CATTCACTTG CTTCATTCCC TGTTTCTGAT 
ACTGAAAGAG CACAAGAACT GTGTTCAACC AACAGTGACA CCATATCTTC AGGTTATCCT 
GAGATGCTGG AGTTGCCTGA CAGGACATTG GGTTTATCAC ATACTGCTTT ACCTCAGCAG 
AATAATCTGA CTGCACATCC AGAACACCTT CATGCACAAA CAAATTTTTT TCACTCTACT 
GAGAAAGCCC AAGAAGGGTT GGTGTTTCCC AGACCATGTC AATTTGAAGA GATGTCTGCT 
GAGCATTTTA TCCAGCCTCA ACATGATGAC TTAAAGGCAC TTCAACAGCA GTTAGATATG 
CAAAGGGAAG CCATTAGATC TGGCCAGGAA ATGCAAGAGA AAATGCTTTT GCAAAGGTTA 
AATAAATTGG AGCAAAGGAT ATCTTCCAAA CAAATTAGCT CCTCTCTATT TTCATCCCAG 
GTAGCACTGC CCATTGCCAA TTCTGACGGG ACCTTACAGT CTTTCCCAAC TAAAAGTAAC 
GAGACTGAGC TGCTTGGGTC CCAAGATGAG TATCTGAGCT TTTCACAGCC TCGTCTGCCT 
CTGCAGAATA ACATGACAGA GCAGTTGGAC TTAGAAAAGG TATTTCATAA AGAACTGCTT 
TTACACAAAC AAAAGAGCCA AAACAAAAGT GAATCTTCTG AGCATTCCCT CCCTCCTTTG 
TTTCTGTCCA AGGAAATAGA GCATCCATTC ATTTCACTAC CTTTTGCAGA AAGTAAATCT 
AAAAGCATTT GTGAGTTGTA TTTATCTGAC AAGAAGCATG CAGCTCCTAA TGATGCTGTG 
ATCCCAAGAT TGCAGGACAG GCTTTTAAGC TGTTCCCAAC CTGTCTTAAC TCAGCAAGAT 
AACATGAGTC TTCAGAAGCA GCTGAATCTA CAAAGAGAAA CTCTACACTC TAGGCAAAAA 
GCCCAAGAAG AATTGCTTGT GCAGAGACAG ACATCCTTAC AGCAGCAAAT CCAAAGACAC 
AGAGAGACTT TGAAAAATTT CTTTAATGTC AGTCAGGCAA GGAATCCCAC AGATGAGAAT 
GACTTGGAAA TGCAGAAGAG AGAACAGCTC GGAGGGTGGT TTCCTCACAC CCAAGGCCTT 
ACCTGGGGAG ACGCAGGTCA GGGGAGCGCT AATGGTGAGC AGCCACGTGC AGATGTCCAT 
GCTGAGCACA ATGGTGAGAG TCTGGCCAAA GAACTGAGTG GTAGAGCCTC AAAGCCACCT 
GTCTCCAAAG TTAAATGTGT CTTGGACTTA AACCAGCATG AGCTTAGTAC TATACAGGAA 
GTAGAGTCAC CAGCAAGTGG CAGGATTTCC ATGCCAGGTA AACAAGGAAA AGCAGAGTTT 
TATCAAGACC GAGACCCATT AAGGGTCTCA GTAAGCAGAG AACAAAGTTT CTTGGAGAGC 
CCACTGGCAC ATGATCCATT TGGTTGTCAC CAACCACCTG CCCAAGAGAA CAGCAAAAGC 
CATGACGACA ATGCTGAAGC AGTTAAAGTC AAGAAATCTG ATGTTGAGGA TCATGCAGTA 
TTGAGTCATG CTGTCTCAAA AGAAGAAGCG TGTACAAACC TGGGTCCACT TGGAAAGCCA 
GACGATGAGG CTGAAACACA AGAGATTTCT CAGGAGCCAT TGTCTTCAGT AACTGTTTCT 
ACTGGAAGCT TCTTAAGTTA TGAAATCACA GATTTGAGCC TCACAGATCC AGAGTCATTT 
TCAGAACAGA CAGAGCATCT AGAACAAGAA TCTACCAATA AGCAGGAAGA GACTGATCCT 
CTTAGTATTG CAGTCCCTTC AGTTATTTAT CAGCAACAGC ATTCCTTAGG TGCTCATAAC 
TCTCTGTTGC CCATGGAGGA AGAAAGTACA TCTGATCATA CACATGTTCA GCAGATCATG 
GACAATGATG TAAATGAAGC AAATTTGATA CCTGACAAAA GAGACTTTCA GGTTCCAGCT 
GTGGACCTTG ACTTTCGAGA ATTGGAACAC ATATTTCCAC ACTTGCATCG TCAGCTCTTT 
AAACCCTTAG AACCACATCT AGATTTTGAC TTATCATCCC CTGGGACATC TCAAGAAGAC 
AGCGACTTTT ACCAGCAGAG CTCAGAATCT TCATCTGAAA AACATGTTAA GGCATTATCC 
ACAGGCACAA TCTGCTTCCC AGCACTGACA GCCAAGTCAC ATTCTCCTAA CCCAAGGCTG 
AACCAACTTG ATATTAACCT GGCTCATGCA ACAACTGAGG GATCTGAACA GTCTTTTCAA 
CAGCTTCGAC CAGAATTCTC TTCACAGGAA AGCCAACATG CTGACCTACC CAGTATTTAT 
AGCATTGAAG CAAGAGGTCC TTCCCAACGC ATGGAAAACC AGAACTATTC TGAAATGTTA 
CAAAATAAGA AAAAAAGTCT CTCTCTCCAG CCCTCAACAG AGGATTTGAC TCCAGCTTGC 
AGTTCATCTG ATACTGCTCT GTTTGATCAG TTACACCTGC AGCACAGCAC TCCGTGTGCT 
TCTGTCTCTA GTGAGTGCTC AGTAAAACTG TTAGAAAGCA GAGAAGAAGT GCTGGGATTT 
GAAGAGCTGT CAAGAAGGGC AGTTACAATG TCACAAAGAC TCACTGAAGA TGAAAATGTA 
GTTCTACCTA TAAACCCACA TGTAGGAAGA GTGGAGAAGG AGGCTTCAGT TCAGGGGTCT 
AATCCATTGA GTATTCAAAA TGAAAAGCCA ATTCAGAACT TCATTGAAAC TGACACTACA 
GAAGCTGTAG GAAATGTATG TCAGCTAGCA CAAGCAGAGC ACATATTAAA ATCCTGTCCA 
TTTCGGAGCC CTATTCCAAT CTGGGAAACG GATACTGGCT ATGGTATAAT GGAAGAACCA 
GATCTCACTC TAGTGAGTAA TAGTGACATC AGCATCACTG AAACAGATCT TGCAAACTTA 
ACCCTTGAAG ACAGAGAAGA CAATGAAGCA CAGTTTTTTC AGGCAGGTGT CGTTCTGCCT 
ACATCTTCAA TGGAAACCTC TGTTTGTGGA GCTGTATCAG AGCCTTATGT GGACCAACCC 
ACAGTGGCAC CTTCAGCTAC ATCAGGTAGT TTACAAGAAG CATTTATGAC AAGACAGACA 
CTTACAGAAC GATCTTACCA GAGGCAGAGA GAGATCTGGA ATAAAACTCG ACTCCCTCAA 
ACCAAAGTAT CCAAAGAGAA GCTACCTACA GGTTGCACAG GCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_011242550.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240852.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X13, mRNA.

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CloneID	OMu74561
Clone ID Related Accession (Same CDS sequence)	XM_011242550.2 , XM_011242550.3
Accession Version	XM_011242550.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242550.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAC TCAAACAGCT GCAGAAAGAG GACCTGGCCC GTAGGAGGCA GACCGTGGCA 
CAGATGCCGC CACAGATGCT GGAGTTGCCA TACCGACGGA GTGAGATGAA GGAAGACAGG 
CAGCGAGAGC TGGAGTTTGC CTTTGAAGAT ATGTACAATG CAGATAGGAA GGTGAAAGGA 
AACCTGATTT TACATCTTAA GCCAGAACCT TTGCCTACAA TCTCTGATCA GCTCCAAGAT 
GAAGAACTGG ACCTTTCAAT GGAACAAGAA AATCAAGTGC CCTTGGCAGC AAAGATCCAA 
CAAATCCCCT CAAGAATTCT TTTTAAAAGA TTATTAAACA AGATTAGAAG TCAAAAATCT 
CTCTGGACAA TTAAATCTGT GTCTGAGGAT GAAGGTGAGG TGACTTCGAG CATCATTGAG 
ATTGAGAGTA AAGTGCCAAG CGTGGACTCG GGAGCCATTA TCACAGAGGA GAGAACAGCG 
GCGTCCTTTG AGCAGGAGCA AGTCACAGAC AGTGATAGGC TGACGATAGA ATCTGGACCA 
CTGAGCAGTG AAGATAAGCC ATTGTACTAC AAAGCGGGGA CTGGAAGGGA ACAAGCAATG 
GCAGTGTCTC CACCTGCCAC AGCTGTGGCT CAGAGTTCAG TCCTGCTTCA TCCTCAGGAA 
GAGGCAGTCA GAATTCGAAT GTCATTAAGG CGCAAACAGA TAATGGAGAT AGAAGAGCAG 
AAGCAGAAGC AGTTGGAATT ACTTGAACAA ATTGAGCAAC AGAAGTTAAG GTTAGAAACT 
GATTGTTTCC GGGCTCAACT GGAAGAGCAA AGAAAGCAAG CTGATCAGCC TGAGGTTTGC 
TGTGCTCCAA TGTCACATGC TATGATTTCT GATGAAGACT CTCACAGGCA GATGATTCGT 
AACTATCAAC ATCAGCTTTT ACAACAAAAC AGGTTACACA AGGAGACTGT TGAAACTGCC 
AGGAAACGAT TACTCGAATA CCAAACTGTA TTAAAAGAAA GATCCCCATC CCTGTCAGCC 
TCAGCACTGG TACCTGATTC TGTTGTGTCA GGGCCACCAC AGCAATCCTA CAAGCCTGCT 
GCTGCTTCAG ACTCTTGGGA TCCAAGCCAG AGACTGAAAC TAAGTCCTAG CAAATATCAA 
CCTGTGCAAC CCTCACAGAT CCCTGCATTA GAGCAGAGTC ATATTCAGGT ACCAAGACAT 
GGTCACATTA CACAGAGGCA AGGAAAAATG GCTGTATCAG AAATGTTAGG TAAGCAGCCC 
GTGGAGTCAC AAGAACGTCA GTGGCAGTTC TCTCAGGTGG AAACACATCA GGGAGACTAT 
GAGTTCGTCC TTAAAGATTC TCATTCACTT TCAAGGACTT TGTCTTATGT TAGGCCACAA 
ACATTACAAG ATGCTAGAGA AGTATCTAAA CCTCCTAGGG TAATAATTTG TCAAAGTCTA 
GATTCCCAAC AGATATCATC AGAGGATAGT GAAAATATAT CTTCTAAGCC AAGTGAACCG 
TCTCCTTTTC TCCCACTGGT GCCTGAACGC CCTTTTACTA GTCTCCCAGT TAAATTCCAT 
TCTGGAACAA TTCACAAACC CTTTACGACC ATAAACCAGT CTGTAATCAG TCAAATGCAT 
GATCAACCTT TGTCATCCTC AGAGACTATT ACAGCTCAGC AGGGTGACTT AAGATTTCTT 
CAAGAACAGT TAGAACTACA GAAGAAAGTT CTTCAGGCAA GACAAGAGGC TCGGGAAAAG 
CTGCTTTTGT GCACACAGAA AGAACTGGGA CAGCAGACTG GCCTCCCAGT GTTCCTCCCT 
TCACCAGCTG GAAATATATT TTCTTCATTA CCCTCTGCCT CAGCTGAATC AGGAAACTTT 
CAGACATCTT CAACAAAAAG TGATGCCACT GTTTCCTCAG ACAATATGGA CAGGCTTTGG 
GATTCTTCAC AACCCATCTC ATCACAGCAA ACTCATTTAG AATTTCTCCA AGAGCAGTCC 
AGTGTTGAGA CGGATAACCT TCAGGCTAGG CGGGAAGCCC AGGAGGTCTT GTTTGCACAT 
ACACAAAATA CGCTGGAAAA AATTGTACGT TCTGAACAAG CTGGGTCTTC TTTGCCACAT 
CAGGTAGCTC AGCAGTCATT TAGTTCACTA ACATTGGCTG ATACACAATC TAAAAAAATC 
CAGAAACAGC CTTTACCTGC AAACAAGAAA GGGCTACTCC CAAGCCAGTC TGAAGTCTCA 
AAAGCTCAGG ATGGATCTTC AGGTTTTCTA CAGCAGACCC TACCTCTCCA GAACACTTTA 
AAGTTGCTTC AAGAGCAGCT GACTAGACAG AGGAGTATGA TTCCGCCTAG ACGTGATGGT 
CAAGAGACAT TACTTTTATA TAAAGAAAGC TGTTCAGAAG ACAGTGAGGC TGGGCCAGTA 
GAATCACTCT CCTCCGTGGT TGTTCAGCAT GCAGATGCTT CTCGTGCAGT TTCAGAAGTA 
CCTAAGCGGC TCCAGGACGT GTATTCATCT GAGGAAGAAA ATAGAGTTCT CTCCAGTCAT 
TTGATCACCC ATGGCTTTCC ACAGCACAGC CTGCAAAGAC AAGAACATTT TACACCACTC 
CAGGAAGAGA CACACATTCA GAGGCTTATA CTTGGTGCTA GAAAAAATAA TGAGGAATTT 
GCTCCCAAGC AAAATGAATT AGAAAAGGGG CTCTGCTCTC AACAGACTGA TGCCTTGTCT 
TCTCCATCCC AAGTCACCGA CTGGGGAACA TCCCGGGGGT CTGTATCAGT CAGAAGTGAT 
AGGACAGACC CCTTAAGGCA TTTTAAGATT CCAGCGTTTC GGGAAAGACT AGTTAGAGTT 
TCACAACATA CATTCCCTCT CCAAGATAAT TTGCAGGAAC ACCAAGAATG GGTAGACACA 
GAAAAAGAGA GCTTTCAATC TAGTCCTCTA ACTCCAGAAA ATCCATCTTC TCAACAGACA 
GGTTTTTCGT CCTTCAAAGC CTCATTAAGA CTGCCATCGT GTGTGTCCTT GCCATCTGCT 
GACTCTGGTA TCACACAGCA CCCTCTCTCA ACAGAGAGTG ACAGTAAAGT CAAGTCAAGC 
CATCTTCAGA TACCAGAACT GCAGCATAGA CTTTCGAAGA TATCCCAGCT CATCCCGCCT 
CAACAAGACA GTTTGAAGGC CCTTCAAGAA CAATTAGCTA CACAAAGGGA AGCCATCATC 
CACTCTAGAC AAGAAGCTCA TGAAGAAACA CTAAGAGAAT GGAAGGAAAA AATATTCCCA 
GAGCAGGTTG GTCCATTTTC TCCCCTGATA CCACAACATT CACTTGCTTC ATTCCCTGTT 
TCTGATACTG AAAGAGCACA AGAACTGTGT TCAACCAACA GTGACACCAT ATCTTCAGGT 
TATCCTGAGA TGCTGGAGTT GCCTGACAGG ACATTGGGTT TATCACATAC TGCTTTACCT 
CAGCAGAATA ATCTGACTGC ACATCCAGAA CACCTTCATG CACAAACAAA TTTTTTTCAC 
TCTACTGAGA AAGCCCAAGA AGGGTTGGTG TTTCCCAGAC CATGTCAATT TGAAGAGATG 
TCTGCTGAGC ATTTTATCCA GCCTCAACAT GATGACTTAA AGGCACTTCA ACAGCAGTTA 
GATATGCAAA GGGAAGCCAT TAGATCTGGC CAGGAAATGC AAGAGAAAAT GCTTTTGCAA 
AGGTTAAATA AATTGGAGCA AAGGATATCT TCCAAACAAA TTAGCTCCTC TCTATTTTCA 
TCCCAGGTAG CACTGCCCAT TGCCAATTCT GACGGGACCT TACAGTCTTT CCCAACTAAA 
AGTAACGAGA CTGAGCTGCT TGGGTCCCAA GATGAGTATC TGAGCTTTTC ACAGCCTCGT 
CTGCCTCTGC AGAATAACAT GACAGAGCAG TTGGACTTAG AAAAGGTATT TCATAAAGAA 
CTGCTTTTAC ACAAACAAAA GAGCCAAAAC AAAAGTGAAT CTTCTGAGCA TTCCCTCCCT 
CCTTTGTTTC TGTCCAAGGA AATAGAGCAT CCATTCATTT CACTACCTTT TGCAGAAAGT 
AAATCTAAAA GCATTTGTGA GTTGTATTTA TCTGACAAGA AGCATGCAGC TCCTAATGAT 
GCTGTGATCC CAAGATTGCA GGACAGGCTT TTAAGCTGTT CCCAACCTGT CTTAACTCAG 
CAAGATAACA TGAGTCTTCA GAAGCAGCTG AATCTACAAA GAGAAACTCT ACACTCTAGG 
CAAAAAGCCC AAGAAGAATT GCTTGTGCAG AGACAGACAT CCTTACAGCA GCAAATCCAA 
AGACACAGAG AGACTTTGAA AAATTTCTTT AATGTCAGTC AGGCAAGGAA TCCCACAGAT 
GAGAATGACT TGGAAATGCA GAAGAGAGAA CAGCTCGGAG GGTGGTTTCC TCACACCCAA 
GGCCTTACCT GGGGAGACGC AGGTCAGGGG AGCGCTAATG GTGAGCAGCC ACGTGCAGAT 
GTCCATGCTG AGCACAATGG TGAGAGTCTG GCCAAAGAAC TGAGTGGTAG AGCCTCAAAG 
CCACCTGTCT CCAAAGTTAA ATGTGTCTTG GACTTAAACC AGCATGAGCT TAGTACTATA 
CAGGAAGTAG AGTCACCAGC AAGTGGCAGG ATTTCCATGC CAGGTAAACA AGGAAAAGCA 
GAGTTTTATC AAGACCGAGA CCCATTAAGG GTCTCAGTAA GCAGAGAACA AAGTTTCTTG 
GAGAGCCCAC TGGCACATGA TCCATTTGGT TGTCACCAAC CACCTGCCCA AGAGAACAGC 
AAAAGCCATG ACGACAATGC TGAAGCAGTT AAAGTCAAGA AATCTGATGT TGAGGATCAT 
GCAGTATTGA GTCATGCTGT CTCAAAAGAA GAAGCGTGTA CAAACCTGGG TCCACTTGGA 
AAGCCAGACG ATGAGGCTGA AACACAAGAG ATTTCTCAGG AGCCATTGTC TTCAGTAACT 
GTTTCTACTG GAAGCTTCTT AAGTTATGAA ATCACAGATT TGAGCCTCAC AGATCCAGAG 
TCATTTTCAG AACAGACAGA GCATCTAGAA CAAGAATCTA CCAATAAGCA GGAAGAGACT 
GATCCTCTTA GTATTGCAGT CCCTTCAGTT ATTTATCAGC AACAGCATTC CTTAGGTGCT 
CATAACTCTC TGTTGCCCAT GGAGGAAGAA AGTACATCTG ATCATACACA TGTTCAGCAG 
ATCATGGACA ATGATGTAAA TGAAGCAAAT TTGATACCTG ACAAAAGAGA CTTTCAGGTT 
CCAGCTGTGG ACCTTGACTT TCGAGAATTG GAACACATAT TTCCACACTT GCATCGTCAG 
CTCTTTAAAC CCTTAGAACC ACATCTAGAT TTTGACTTAT CATCCCCTGG GACATCTCAA 
GAAGACAGCG ACTTTTACCA GCAGAGCTCA GAATCTTCAT CTGAAAAACA TGTTAAGGCA 
TTATCCACAG GCACAATCTG CTTCCCAGCA CTGACAGCCA AGTCACATTC TCCTAACCCA 
AGGCTGAACC AACTTGATAT TAACCTGGCT CATGCAACAA CTGAGGGATC TGAACAGTCT 
TTTCAACAGC TTCGACCAGA ATTCTCTTCA CAGGAAAGCC AACATGCTGA CCTACCCAGT 
ATTTATAGCA TTGAAGCAAG AGGTCCTTCC CAACGCATGG AAAACCAGAA CTATTCTGAA 
ATGTTACAAA ATAAGAAAAA AAGTCTCTCT CTCCAGCCCT CAACAGAGGA TTTGACTCCA 
GCTTGCAGTT CATCTGATAC TGCTCTGTTT GATCAGTTAC ACCTGCAGCA CAGCACTCCG 
TGTGCTTCTG TCTCTAGTGA GTGCTCAGTA AAACTGTTAG AAAGCAGAGA AGAAGTGCTG 
GGATTTGAAG AGCTGTCAAG AAGGGCAGTT ACAATGTCAC AAAGACTCAC TGAAGATGAA 
AATGTAGTTC TACCTATAAA CCCACATGTA GGAAGAGTGG AGAAGGAGGC TTCAGTTCAG 
GGGTCTAATC CATTGAGTAT TCAAAATGAA AAGCCAATTC AGAACTTCAT TGAAACTGAC 
ACTACAGAAG CTGTAGGAAA TGTATGTCAG CTAGCACAAG CAGAGCACAT ATTAAAATCC 
TGTCCATTTC GGAGCCCTAT TCCAATCTGG GAAACGGATA CTGGCTATGG TATAATGGAA 
GAACCAGATC TCACTCTAGT GAGTAATAGT GACATCAGCA TCACTGAAAC AGATCTTGCA 
AACTTAACCC TTGAAGACAG AGAAGACAAT GAAGCACAGT TTTTTCAGGC AGGTGTCGTT 
CTGCCTACAT CTTCAATGGA AACCTCTGTT TGTGGAGCTG TATCAGAGCC TTATGTGGAC 
CAACCCACAG TGGCACCTTC AGCTACATCA GGTAGTTTAC AAGAAGCATT TATGACAAGA 
CAGACACTTA CAGAACGATC TTACCAGAGG CAGAGAGAGA TCTGGAATAA AACTCGACTC 
CCTCAAACCA AAGTATCCAA AGAGAAGCTA CCTACAGGTT GCACAGGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240852.1
CDS	98..6571
Translation	MEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_011242550.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242550.3

ORF Insert Sequence:

ATGGAAGAAC TCAAACAGCT GCAGAAAGAG GACCTGGCCC GTAGGAGGCA GACCGTGGCA 
CAGATGCCGC CACAGATGCT GGAGTTGCCA TACCGACGGA GTGAGATGAA GGAAGACAGG 
CAGCGAGAGC TGGAGTTTGC CTTTGAAGAT ATGTACAATG CAGATAGGAA GGTGAAAGGA 
AACCTGATTT TACATCTTAA GCCAGAACCT TTGCCTACAA TCTCTGATCA GCTCCAAGAT 
GAAGAACTGG ACCTTTCAAT GGAACAAGAA AATCAAGTGC CCTTGGCAGC AAAGATCCAA 
CAAATCCCCT CAAGAATTCT TTTTAAAAGA TTATTAAACA AGATTAGAAG TCAAAAATCT 
CTCTGGACAA TTAAATCTGT GTCTGAGGAT GAAGGTGAGG TGACTTCGAG CATCATTGAG 
ATTGAGAGTA AAGTGCCAAG CGTGGACTCG GGAGCCATTA TCACAGAGGA GAGAACAGCG 
GCGTCCTTTG AGCAGGAGCA AGTCACAGAC AGTGATAGGC TGACGATAGA ATCTGGACCA 
CTGAGCAGTG AAGATAAGCC ATTGTACTAC AAAGCGGGGA CTGGAAGGGA ACAAGCAATG 
GCAGTGTCTC CACCTGCCAC AGCTGTGGCT CAGAGTTCAG TCCTGCTTCA TCCTCAGGAA 
GAGGCAGTCA GAATTCGAAT GTCATTAAGG CGCAAACAGA TAATGGAGAT AGAAGAGCAG 
AAGCAGAAGC AGTTGGAATT ACTTGAACAA ATTGAGCAAC AGAAGTTAAG GTTAGAAACT 
GATTGTTTCC GGGCTCAACT GGAAGAGCAA AGAAAGCAAG CTGATCAGCC TGAGGTTTGC 
TGTGCTCCAA TGTCACATGC TATGATTTCT GATGAAGACT CTCACAGGCA GATGATTCGT 
AACTATCAAC ATCAGCTTTT ACAACAAAAC AGGTTACACA AGGAGACTGT TGAAACTGCC 
AGGAAACGAT TACTCGAATA CCAAACTGTA TTAAAAGAAA GATCCCCATC CCTGTCAGCC 
TCAGCACTGG TACCTGATTC TGTTGTGTCA GGGCCACCAC AGCAATCCTA CAAGCCTGCT 
GCTGCTTCAG ACTCTTGGGA TCCAAGCCAG AGACTGAAAC TAAGTCCTAG CAAATATCAA 
CCTGTGCAAC CCTCACAGAT CCCTGCATTA GAGCAGAGTC ATATTCAGGT ACCAAGACAT 
GGTCACATTA CACAGAGGCA AGGAAAAATG GCTGTATCAG AAATGTTAGG TAAGCAGCCC 
GTGGAGTCAC AAGAACGTCA GTGGCAGTTC TCTCAGGTGG AAACACATCA GGGAGACTAT 
GAGTTCGTCC TTAAAGATTC TCATTCACTT TCAAGGACTT TGTCTTATGT TAGGCCACAA 
ACATTACAAG ATGCTAGAGA AGTATCTAAA CCTCCTAGGG TAATAATTTG TCAAAGTCTA 
GATTCCCAAC AGATATCATC AGAGGATAGT GAAAATATAT CTTCTAAGCC AAGTGAACCG 
TCTCCTTTTC TCCCACTGGT GCCTGAACGC CCTTTTACTA GTCTCCCAGT TAAATTCCAT 
TCTGGAACAA TTCACAAACC CTTTACGACC ATAAACCAGT CTGTAATCAG TCAAATGCAT 
GATCAACCTT TGTCATCCTC AGAGACTATT ACAGCTCAGC AGGGTGACTT AAGATTTCTT 
CAAGAACAGT TAGAACTACA GAAGAAAGTT CTTCAGGCAA GACAAGAGGC TCGGGAAAAG 
CTGCTTTTGT GCACACAGAA AGAACTGGGA CAGCAGACTG GCCTCCCAGT GTTCCTCCCT 
TCACCAGCTG GAAATATATT TTCTTCATTA CCCTCTGCCT CAGCTGAATC AGGAAACTTT 
CAGACATCTT CAACAAAAAG TGATGCCACT GTTTCCTCAG ACAATATGGA CAGGCTTTGG 
GATTCTTCAC AACCCATCTC ATCACAGCAA ACTCATTTAG AATTTCTCCA AGAGCAGTCC 
AGTGTTGAGA CGGATAACCT TCAGGCTAGG CGGGAAGCCC AGGAGGTCTT GTTTGCACAT 
ACACAAAATA CGCTGGAAAA AATTGTACGT TCTGAACAAG CTGGGTCTTC TTTGCCACAT 
CAGGTAGCTC AGCAGTCATT TAGTTCACTA ACATTGGCTG ATACACAATC TAAAAAAATC 
CAGAAACAGC CTTTACCTGC AAACAAGAAA GGGCTACTCC CAAGCCAGTC TGAAGTCTCA 
AAAGCTCAGG ATGGATCTTC AGGTTTTCTA CAGCAGACCC TACCTCTCCA GAACACTTTA 
AAGTTGCTTC AAGAGCAGCT GACTAGACAG AGGAGTATGA TTCCGCCTAG ACGTGATGGT 
CAAGAGACAT TACTTTTATA TAAAGAAAGC TGTTCAGAAG ACAGTGAGGC TGGGCCAGTA 
GAATCACTCT CCTCCGTGGT TGTTCAGCAT GCAGATGCTT CTCGTGCAGT TTCAGAAGTA 
CCTAAGCGGC TCCAGGACGT GTATTCATCT GAGGAAGAAA ATAGAGTTCT CTCCAGTCAT 
TTGATCACCC ATGGCTTTCC ACAGCACAGC CTGCAAAGAC AAGAACATTT TACACCACTC 
CAGGAAGAGA CACACATTCA GAGGCTTATA CTTGGTGCTA GAAAAAATAA TGAGGAATTT 
GCTCCCAAGC AAAATGAATT AGAAAAGGGG CTCTGCTCTC AACAGACTGA TGCCTTGTCT 
TCTCCATCCC AAGTCACCGA CTGGGGAACA TCCCGGGGGT CTGTATCAGT CAGAAGTGAT 
AGGACAGACC CCTTAAGGCA TTTTAAGATT CCAGCGTTTC GGGAAAGACT AGTTAGAGTT 
TCACAACATA CATTCCCTCT CCAAGATAAT TTGCAGGAAC ACCAAGAATG GGTAGACACA 
GAAAAAGAGA GCTTTCAATC TAGTCCTCTA ACTCCAGAAA ATCCATCTTC TCAACAGACA 
GGTTTTTCGT CCTTCAAAGC CTCATTAAGA CTGCCATCGT GTGTGTCCTT GCCATCTGCT 
GACTCTGGTA TCACACAGCA CCCTCTCTCA ACAGAGAGTG ACAGTAAAGT CAAGTCAAGC 
CATCTTCAGA TACCAGAACT GCAGCATAGA CTTTCGAAGA TATCCCAGCT CATCCCGCCT 
CAACAAGACA GTTTGAAGGC CCTTCAAGAA CAATTAGCTA CACAAAGGGA AGCCATCATC 
CACTCTAGAC AAGAAGCTCA TGAAGAAACA CTAAGAGAAT GGAAGGAAAA AATATTCCCA 
GAGCAGGTTG GTCCATTTTC TCCCCTGATA CCACAACATT CACTTGCTTC ATTCCCTGTT 
TCTGATACTG AAAGAGCACA AGAACTGTGT TCAACCAACA GTGACACCAT ATCTTCAGGT 
TATCCTGAGA TGCTGGAGTT GCCTGACAGG ACATTGGGTT TATCACATAC TGCTTTACCT 
CAGCAGAATA ATCTGACTGC ACATCCAGAA CACCTTCATG CACAAACAAA TTTTTTTCAC 
TCTACTGAGA AAGCCCAAGA AGGGTTGGTG TTTCCCAGAC CATGTCAATT TGAAGAGATG 
TCTGCTGAGC ATTTTATCCA GCCTCAACAT GATGACTTAA AGGCACTTCA ACAGCAGTTA 
GATATGCAAA GGGAAGCCAT TAGATCTGGC CAGGAAATGC AAGAGAAAAT GCTTTTGCAA 
AGGTTAAATA AATTGGAGCA AAGGATATCT TCCAAACAAA TTAGCTCCTC TCTATTTTCA 
TCCCAGGTAG CACTGCCCAT TGCCAATTCT GACGGGACCT TACAGTCTTT CCCAACTAAA 
AGTAACGAGA CTGAGCTGCT TGGGTCCCAA GATGAGTATC TGAGCTTTTC ACAGCCTCGT 
CTGCCTCTGC AGAATAACAT GACAGAGCAG TTGGACTTAG AAAAGGTATT TCATAAAGAA 
CTGCTTTTAC ACAAACAAAA GAGCCAAAAC AAAAGTGAAT CTTCTGAGCA TTCCCTCCCT 
CCTTTGTTTC TGTCCAAGGA AATAGAGCAT CCATTCATTT CACTACCTTT TGCAGAAAGT 
AAATCTAAAA GCATTTGTGA GTTGTATTTA TCTGACAAGA AGCATGCAGC TCCTAATGAT 
GCTGTGATCC CAAGATTGCA GGACAGGCTT TTAAGCTGTT CCCAACCTGT CTTAACTCAG 
CAAGATAACA TGAGTCTTCA GAAGCAGCTG AATCTACAAA GAGAAACTCT ACACTCTAGG 
CAAAAAGCCC AAGAAGAATT GCTTGTGCAG AGACAGACAT CCTTACAGCA GCAAATCCAA 
AGACACAGAG AGACTTTGAA AAATTTCTTT AATGTCAGTC AGGCAAGGAA TCCCACAGAT 
GAGAATGACT TGGAAATGCA GAAGAGAGAA CAGCTCGGAG GGTGGTTTCC TCACACCCAA 
GGCCTTACCT GGGGAGACGC AGGTCAGGGG AGCGCTAATG GTGAGCAGCC ACGTGCAGAT 
GTCCATGCTG AGCACAATGG TGAGAGTCTG GCCAAAGAAC TGAGTGGTAG AGCCTCAAAG 
CCACCTGTCT CCAAAGTTAA ATGTGTCTTG GACTTAAACC AGCATGAGCT TAGTACTATA 
CAGGAAGTAG AGTCACCAGC AAGTGGCAGG ATTTCCATGC CAGGTAAACA AGGAAAAGCA 
GAGTTTTATC AAGACCGAGA CCCATTAAGG GTCTCAGTAA GCAGAGAACA AAGTTTCTTG 
GAGAGCCCAC TGGCACATGA TCCATTTGGT TGTCACCAAC CACCTGCCCA AGAGAACAGC 
AAAAGCCATG ACGACAATGC TGAAGCAGTT AAAGTCAAGA AATCTGATGT TGAGGATCAT 
GCAGTATTGA GTCATGCTGT CTCAAAAGAA GAAGCGTGTA CAAACCTGGG TCCACTTGGA 
AAGCCAGACG ATGAGGCTGA AACACAAGAG ATTTCTCAGG AGCCATTGTC TTCAGTAACT 
GTTTCTACTG GAAGCTTCTT AAGTTATGAA ATCACAGATT TGAGCCTCAC AGATCCAGAG 
TCATTTTCAG AACAGACAGA GCATCTAGAA CAAGAATCTA CCAATAAGCA GGAAGAGACT 
GATCCTCTTA GTATTGCAGT CCCTTCAGTT ATTTATCAGC AACAGCATTC CTTAGGTGCT 
CATAACTCTC TGTTGCCCAT GGAGGAAGAA AGTACATCTG ATCATACACA TGTTCAGCAG 
ATCATGGACA ATGATGTAAA TGAAGCAAAT TTGATACCTG ACAAAAGAGA CTTTCAGGTT 
CCAGCTGTGG ACCTTGACTT TCGAGAATTG GAACACATAT TTCCACACTT GCATCGTCAG 
CTCTTTAAAC CCTTAGAACC ACATCTAGAT TTTGACTTAT CATCCCCTGG GACATCTCAA 
GAAGACAGCG ACTTTTACCA GCAGAGCTCA GAATCTTCAT CTGAAAAACA TGTTAAGGCA 
TTATCCACAG GCACAATCTG CTTCCCAGCA CTGACAGCCA AGTCACATTC TCCTAACCCA 
AGGCTGAACC AACTTGATAT TAACCTGGCT CATGCAACAA CTGAGGGATC TGAACAGTCT 
TTTCAACAGC TTCGACCAGA ATTCTCTTCA CAGGAAAGCC AACATGCTGA CCTACCCAGT 
ATTTATAGCA TTGAAGCAAG AGGTCCTTCC CAACGCATGG AAAACCAGAA CTATTCTGAA 
ATGTTACAAA ATAAGAAAAA AAGTCTCTCT CTCCAGCCCT CAACAGAGGA TTTGACTCCA 
GCTTGCAGTT CATCTGATAC TGCTCTGTTT GATCAGTTAC ACCTGCAGCA CAGCACTCCG 
TGTGCTTCTG TCTCTAGTGA GTGCTCAGTA AAACTGTTAG AAAGCAGAGA AGAAGTGCTG 
GGATTTGAAG AGCTGTCAAG AAGGGCAGTT ACAATGTCAC AAAGACTCAC TGAAGATGAA 
AATGTAGTTC TACCTATAAA CCCACATGTA GGAAGAGTGG AGAAGGAGGC TTCAGTTCAG 
GGGTCTAATC CATTGAGTAT TCAAAATGAA AAGCCAATTC AGAACTTCAT TGAAACTGAC 
ACTACAGAAG CTGTAGGAAA TGTATGTCAG CTAGCACAAG CAGAGCACAT ATTAAAATCC 
TGTCCATTTC GGAGCCCTAT TCCAATCTGG GAAACGGATA CTGGCTATGG TATAATGGAA 
GAACCAGATC TCACTCTAGT GAGTAATAGT GACATCAGCA TCACTGAAAC AGATCTTGCA 
AACTTAACCC TTGAAGACAG AGAAGACAAT GAAGCACAGT TTTTTCAGGC AGGTGTCGTT 
CTGCCTACAT CTTCAATGGA AACCTCTGTT TGTGGAGCTG TATCAGAGCC TTATGTGGAC 
CAACCCACAG TGGCACCTTC AGCTACATCA GGTAGTTTAC AAGAAGCATT TATGACAAGA 
CAGACACTTA CAGAACGATC TTACCAGAGG CAGAGAGAGA TCTGGAATAA AACTCGACTC 
CCTCAAACCA AAGTATCCAA AGAGAAGCTA CCTACAGGTT GCACAGGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_017313419.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168908.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X1, mRNA.

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CloneID	OMu82850
Clone ID Related Accession (Same CDS sequence)	XM_017313419.1 , XM_017313419.2
Accession Version	XM_017313419.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGTAAAC AAGGAAAAGC AGAGTTTTAT CAAGACCGAG ACCCATTAAG GGTCTCAGTA 
AGCAGAGAAC AAAGTTTCTT GGAGAGCCCA CTGGCACATG ATCCATTTGG TTGTCACCAA 
CCACCTGCCC AAGAGAACAG CAAAAGCCAT GACGACAATG CTGAAGCAGT TAAAGTCAAG 
AAATCTGATG TTGAGGATCA TGCAGTATTG AGTCATGCTG TCTCAAAAGA AGAAGCGTGT 
ACAAACCTGG GTCCACTTGG AAAGCCAGAC GATGAGGCTG AAACACAAGA GATTTCTCAG 
GAGCCATTGT CTTCAGTAAC TGTTTCTACT GGAAGCTTCT TAAGTTATGA AATCACAGAT 
TTGAGCCTCA CAGATCCAGA GTCATTTTCA GAACAGACAG AGCATCTAGA ACAAGAATCT 
ACCAATAAGC AGGAAGAGAC TGATCCTCTT AGTATTGCAG TCCCTTCAGT TATTTATCAG 
CAACAGCATT CCTTAGGTGC TCATAACTCT CTGTTGCCCA TGGAGGAAGA AAGTACATCT 
GATCATACAC ATGTTCAGCA GATCATGGAC AATGATGTAA ATGAAGCAAA TTTGATACCT 
GACAAAAGAG ACTTTCAGGT TCCAGCTGTG GACCTTGACT TTCGAGAATT GGAACACATA 
TTTCCACACT TGCATCGTCA GCTCTTTAAA CCCTTAGAAC CACATCTAGA TTTTGACTTA 
TCATCCCCTG GGACATCTCA AGAAGACAGC GACTTTTACC AGCAGAGCTC AGAATCTTCA 
TCTGAAAAAC ATGTTAAGGC ATTATCCACA GGCACAATCT GCTTCCCAGC ACTGACAGCC 
AAGTCACATT CTCCTAACCC AAGGCTGAAC CAACTTGATA TTAACCTGGC TCATGCAACA 
ACTGAGGGAT CTGAACAGTC TTTTCAACAG CTTCGACCAG AATTCTCTTC ACAGGAAAGC 
CAACATGCTG ACCTACCCAG TATTTATAGC ATTGAAGCAA GAGGTCCTTC CCAACGCATG 
GAAAACCAGA ACTATTCTGA AATGTTACAA AATAAGAAAA AAAGTCTCTC TCTCCAGCCC 
TCAACAGAGG ATTTGACTCC AGCTTGCAGT TCATCTGATA CTGCTCTGTT TGATCAGTTA 
CACCTGCAGC ACAGCACTCC GTGTGCTTCT GTCTCTAGTG AGTGCTCAGT AAAACTGTTA 
GAAAGCAGAG AAGAAGTGCT GGGATTTGAA GAGCTGTCAA GAAGGGCAGT TACAATGTCA 
CAAAGACTCA CTGAAGATGA AAATGTAGTT CTACCTATAA ACCCACATGT AGGAAGAGTG 
GAGAAGGAGG CTTCAGTTCA GGGGTCTAAT CCATTGAGTA TTCAAAATGA AAAGCCAATT 
CAGAACTTCA TTGAAACTGA CACTACAGAA GCTGTAGGAA ATGTATGTCA GCTAGCACAA 
GCAGAGCACA TATTAAAATC CTGTCCATTT CGGAGCCCTA TTCCAATCTG GGAAACGGAT 
ACTGGCTATG GTATAATGGA AGAACCAGAT CTCACTCTAG TGAGTAATAG TGACATCAGC 
ATCACTGAAA CAGATCTTGC AAACTTAACC CTTGAAGACA GAGAAGACAA TGAAGCACAG 
TTTTTTCAGG CAGGTGTCGT TCTGCCTACA TCTTCAATGG AAACCTCTGT TTGTGGAGCT 
GTATCAGAGC CTTATGTGGA CCAACCCACA GTGGCACCTT CAGCTACATC AGGTAGTTTA 
CAAGAAGCAT TTATGACAAG ACAGACACTT ACAGAACGAT CTTACCAGAG GCAGAGAGAG 
ATCTGGAATA AAACTCGACT CCCTCAAACC AAAGTATCCA AAGAGAAGCT ACCTACAGGT 
TGCACAGGCA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168908.1
CDS	77..7471
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_017313419.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313419.1

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGTAAAC AAGGAAAAGC AGAGTTTTAT CAAGACCGAG ACCCATTAAG GGTCTCAGTA 
AGCAGAGAAC AAAGTTTCTT GGAGAGCCCA CTGGCACATG ATCCATTTGG TTGTCACCAA 
CCACCTGCCC AAGAGAACAG CAAAAGCCAT GACGACAATG CTGAAGCAGT TAAAGTCAAG 
AAATCTGATG TTGAGGATCA TGCAGTATTG AGTCATGCTG TCTCAAAAGA AGAAGCGTGT 
ACAAACCTGG GTCCACTTGG AAAGCCAGAC GATGAGGCTG AAACACAAGA GATTTCTCAG 
GAGCCATTGT CTTCAGTAAC TGTTTCTACT GGAAGCTTCT TAAGTTATGA AATCACAGAT 
TTGAGCCTCA CAGATCCAGA GTCATTTTCA GAACAGACAG AGCATCTAGA ACAAGAATCT 
ACCAATAAGC AGGAAGAGAC TGATCCTCTT AGTATTGCAG TCCCTTCAGT TATTTATCAG 
CAACAGCATT CCTTAGGTGC TCATAACTCT CTGTTGCCCA TGGAGGAAGA AAGTACATCT 
GATCATACAC ATGTTCAGCA GATCATGGAC AATGATGTAA ATGAAGCAAA TTTGATACCT 
GACAAAAGAG ACTTTCAGGT TCCAGCTGTG GACCTTGACT TTCGAGAATT GGAACACATA 
TTTCCACACT TGCATCGTCA GCTCTTTAAA CCCTTAGAAC CACATCTAGA TTTTGACTTA 
TCATCCCCTG GGACATCTCA AGAAGACAGC GACTTTTACC AGCAGAGCTC AGAATCTTCA 
TCTGAAAAAC ATGTTAAGGC ATTATCCACA GGCACAATCT GCTTCCCAGC ACTGACAGCC 
AAGTCACATT CTCCTAACCC AAGGCTGAAC CAACTTGATA TTAACCTGGC TCATGCAACA 
ACTGAGGGAT CTGAACAGTC TTTTCAACAG CTTCGACCAG AATTCTCTTC ACAGGAAAGC 
CAACATGCTG ACCTACCCAG TATTTATAGC ATTGAAGCAA GAGGTCCTTC CCAACGCATG 
GAAAACCAGA ACTATTCTGA AATGTTACAA AATAAGAAAA AAAGTCTCTC TCTCCAGCCC 
TCAACAGAGG ATTTGACTCC AGCTTGCAGT TCATCTGATA CTGCTCTGTT TGATCAGTTA 
CACCTGCAGC ACAGCACTCC GTGTGCTTCT GTCTCTAGTG AGTGCTCAGT AAAACTGTTA 
GAAAGCAGAG AAGAAGTGCT GGGATTTGAA GAGCTGTCAA GAAGGGCAGT TACAATGTCA 
CAAAGACTCA CTGAAGATGA AAATGTAGTT CTACCTATAA ACCCACATGT AGGAAGAGTG 
GAGAAGGAGG CTTCAGTTCA GGGGTCTAAT CCATTGAGTA TTCAAAATGA AAAGCCAATT 
CAGAACTTCA TTGAAACTGA CACTACAGAA GCTGTAGGAA ATGTATGTCA GCTAGCACAA 
GCAGAGCACA TATTAAAATC CTGTCCATTT CGGAGCCCTA TTCCAATCTG GGAAACGGAT 
ACTGGCTATG GTATAATGGA AGAACCAGAT CTCACTCTAG TGAGTAATAG TGACATCAGC 
ATCACTGAAA CAGATCTTGC AAACTTAACC CTTGAAGACA GAGAAGACAA TGAAGCACAG 
TTTTTTCAGG CAGGTGTCGT TCTGCCTACA TCTTCAATGG AAACCTCTGT TTGTGGAGCT 
GTATCAGAGC CTTATGTGGA CCAACCCACA GTGGCACCTT CAGCTACATC AGGTAGTTTA 
CAAGAAGCAT TTATGACAAG ACAGACACTT ACAGAACGAT CTTACCAGAG GCAGAGAGAG 
ATCTGGAATA AAACTCGACT CCCTCAAACC AAAGTATCCA AAGAGAAGCT ACCTACAGGT 
TGCACAGGCA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_017313419.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168908.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X1, mRNA.

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CloneID	OMu82850
Clone ID Related Accession (Same CDS sequence)	XM_017313419.1 , XM_017313419.2
Accession Version	XM_017313419.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017313419.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGTAAAC AAGGAAAAGC AGAGTTTTAT CAAGACCGAG ACCCATTAAG GGTCTCAGTA 
AGCAGAGAAC AAAGTTTCTT GGAGAGCCCA CTGGCACATG ATCCATTTGG TTGTCACCAA 
CCACCTGCCC AAGAGAACAG CAAAAGCCAT GACGACAATG CTGAAGCAGT TAAAGTCAAG 
AAATCTGATG TTGAGGATCA TGCAGTATTG AGTCATGCTG TCTCAAAAGA AGAAGCGTGT 
ACAAACCTGG GTCCACTTGG AAAGCCAGAC GATGAGGCTG AAACACAAGA GATTTCTCAG 
GAGCCATTGT CTTCAGTAAC TGTTTCTACT GGAAGCTTCT TAAGTTATGA AATCACAGAT 
TTGAGCCTCA CAGATCCAGA GTCATTTTCA GAACAGACAG AGCATCTAGA ACAAGAATCT 
ACCAATAAGC AGGAAGAGAC TGATCCTCTT AGTATTGCAG TCCCTTCAGT TATTTATCAG 
CAACAGCATT CCTTAGGTGC TCATAACTCT CTGTTGCCCA TGGAGGAAGA AAGTACATCT 
GATCATACAC ATGTTCAGCA GATCATGGAC AATGATGTAA ATGAAGCAAA TTTGATACCT 
GACAAAAGAG ACTTTCAGGT TCCAGCTGTG GACCTTGACT TTCGAGAATT GGAACACATA 
TTTCCACACT TGCATCGTCA GCTCTTTAAA CCCTTAGAAC CACATCTAGA TTTTGACTTA 
TCATCCCCTG GGACATCTCA AGAAGACAGC GACTTTTACC AGCAGAGCTC AGAATCTTCA 
TCTGAAAAAC ATGTTAAGGC ATTATCCACA GGCACAATCT GCTTCCCAGC ACTGACAGCC 
AAGTCACATT CTCCTAACCC AAGGCTGAAC CAACTTGATA TTAACCTGGC TCATGCAACA 
ACTGAGGGAT CTGAACAGTC TTTTCAACAG CTTCGACCAG AATTCTCTTC ACAGGAAAGC 
CAACATGCTG ACCTACCCAG TATTTATAGC ATTGAAGCAA GAGGTCCTTC CCAACGCATG 
GAAAACCAGA ACTATTCTGA AATGTTACAA AATAAGAAAA AAAGTCTCTC TCTCCAGCCC 
TCAACAGAGG ATTTGACTCC AGCTTGCAGT TCATCTGATA CTGCTCTGTT TGATCAGTTA 
CACCTGCAGC ACAGCACTCC GTGTGCTTCT GTCTCTAGTG AGTGCTCAGT AAAACTGTTA 
GAAAGCAGAG AAGAAGTGCT GGGATTTGAA GAGCTGTCAA GAAGGGCAGT TACAATGTCA 
CAAAGACTCA CTGAAGATGA AAATGTAGTT CTACCTATAA ACCCACATGT AGGAAGAGTG 
GAGAAGGAGG CTTCAGTTCA GGGGTCTAAT CCATTGAGTA TTCAAAATGA AAAGCCAATT 
CAGAACTTCA TTGAAACTGA CACTACAGAA GCTGTAGGAA ATGTATGTCA GCTAGCACAA 
GCAGAGCACA TATTAAAATC CTGTCCATTT CGGAGCCCTA TTCCAATCTG GGAAACGGAT 
ACTGGCTATG GTATAATGGA AGAACCAGAT CTCACTCTAG TGAGTAATAG TGACATCAGC 
ATCACTGAAA CAGATCTTGC AAACTTAACC CTTGAAGACA GAGAAGACAA TGAAGCACAG 
TTTTTTCAGG CAGGTGTCGT TCTGCCTACA TCTTCAATGG AAACCTCTGT TTGTGGAGCT 
GTATCAGAGC CTTATGTGGA CCAACCCACA GTGGCACCTT CAGCTACATC AGGTAGTTTA 
CAAGAAGCAT TTATGACAAG ACAGACACTT ACAGAACGAT CTTACCAGAG GCAGAGAGAG 
ATCTGGAATA AAACTCGACT CCCTCAAACC AAAGTATCCA AAGAGAAGCT ACCTACAGGT 
TGCACAGGCA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168908.1
CDS	107..7501
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_017313419.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313419.2

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGTAAAC AAGGAAAAGC AGAGTTTTAT CAAGACCGAG ACCCATTAAG GGTCTCAGTA 
AGCAGAGAAC AAAGTTTCTT GGAGAGCCCA CTGGCACATG ATCCATTTGG TTGTCACCAA 
CCACCTGCCC AAGAGAACAG CAAAAGCCAT GACGACAATG CTGAAGCAGT TAAAGTCAAG 
AAATCTGATG TTGAGGATCA TGCAGTATTG AGTCATGCTG TCTCAAAAGA AGAAGCGTGT 
ACAAACCTGG GTCCACTTGG AAAGCCAGAC GATGAGGCTG AAACACAAGA GATTTCTCAG 
GAGCCATTGT CTTCAGTAAC TGTTTCTACT GGAAGCTTCT TAAGTTATGA AATCACAGAT 
TTGAGCCTCA CAGATCCAGA GTCATTTTCA GAACAGACAG AGCATCTAGA ACAAGAATCT 
ACCAATAAGC AGGAAGAGAC TGATCCTCTT AGTATTGCAG TCCCTTCAGT TATTTATCAG 
CAACAGCATT CCTTAGGTGC TCATAACTCT CTGTTGCCCA TGGAGGAAGA AAGTACATCT 
GATCATACAC ATGTTCAGCA GATCATGGAC AATGATGTAA ATGAAGCAAA TTTGATACCT 
GACAAAAGAG ACTTTCAGGT TCCAGCTGTG GACCTTGACT TTCGAGAATT GGAACACATA 
TTTCCACACT TGCATCGTCA GCTCTTTAAA CCCTTAGAAC CACATCTAGA TTTTGACTTA 
TCATCCCCTG GGACATCTCA AGAAGACAGC GACTTTTACC AGCAGAGCTC AGAATCTTCA 
TCTGAAAAAC ATGTTAAGGC ATTATCCACA GGCACAATCT GCTTCCCAGC ACTGACAGCC 
AAGTCACATT CTCCTAACCC AAGGCTGAAC CAACTTGATA TTAACCTGGC TCATGCAACA 
ACTGAGGGAT CTGAACAGTC TTTTCAACAG CTTCGACCAG AATTCTCTTC ACAGGAAAGC 
CAACATGCTG ACCTACCCAG TATTTATAGC ATTGAAGCAA GAGGTCCTTC CCAACGCATG 
GAAAACCAGA ACTATTCTGA AATGTTACAA AATAAGAAAA AAAGTCTCTC TCTCCAGCCC 
TCAACAGAGG ATTTGACTCC AGCTTGCAGT TCATCTGATA CTGCTCTGTT TGATCAGTTA 
CACCTGCAGC ACAGCACTCC GTGTGCTTCT GTCTCTAGTG AGTGCTCAGT AAAACTGTTA 
GAAAGCAGAG AAGAAGTGCT GGGATTTGAA GAGCTGTCAA GAAGGGCAGT TACAATGTCA 
CAAAGACTCA CTGAAGATGA AAATGTAGTT CTACCTATAA ACCCACATGT AGGAAGAGTG 
GAGAAGGAGG CTTCAGTTCA GGGGTCTAAT CCATTGAGTA TTCAAAATGA AAAGCCAATT 
CAGAACTTCA TTGAAACTGA CACTACAGAA GCTGTAGGAA ATGTATGTCA GCTAGCACAA 
GCAGAGCACA TATTAAAATC CTGTCCATTT CGGAGCCCTA TTCCAATCTG GGAAACGGAT 
ACTGGCTATG GTATAATGGA AGAACCAGAT CTCACTCTAG TGAGTAATAG TGACATCAGC 
ATCACTGAAA CAGATCTTGC AAACTTAACC CTTGAAGACA GAGAAGACAA TGAAGCACAG 
TTTTTTCAGG CAGGTGTCGT TCTGCCTACA TCTTCAATGG AAACCTCTGT TTGTGGAGCT 
GTATCAGAGC CTTATGTGGA CCAACCCACA GTGGCACCTT CAGCTACATC AGGTAGTTTA 
CAAGAAGCAT TTATGACAAG ACAGACACTT ACAGAACGAT CTTACCAGAG GCAGAGAGAG 
ATCTGGAATA AAACTCGACT CCCTCAAACC AAAGTATCCA AAGAGAAGCT ACCTACAGGT 
TGCACAGGCA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_017313420.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168909.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X4, mRNA.

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CloneID	OMu82851
Clone ID Related Accession (Same CDS sequence)	XM_017313420.1 , XM_017313420.2
Accession Version	XM_017313420.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG AGTCATTTTC AGAACAGACA GAGCATCTAG AACAAGAATC TACCAATAAG 
CAGGAAGAGA CTGATCCTCT TAGTATTGCA GTCCCTTCAG TTATTTATCA GCAACAGCAT 
TCCTTAGGTG CTCATAACTC TCTGTTGCCC ATGGAGGAAG AAAGTACATC TGATCATACA 
CATGTTCAGC AGATCATGGA CAATGATGTA AATGAAGCAA ATTTGATACC TGACAAAAGA 
GACTTTCAGG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGAGCTCAG AATCTTCATC TGAAAAACAT 
GTTAAGGCAT TATCCACAGG CACAATCTGC TTCCCAGCAC TGACAGCCAA GTCACATTCT 
CCTAACCCAA GGCTGAACCA ACTTGATATT AACCTGGCTC ATGCAACAAC TGAGGGATCT 
GAACAGTCTT TTCAACAGCT TCGACCAGAA TTCTCTTCAC AGGAAAGCCA ACATGCTGAC 
CTACCCAGTA TTTATAGCAT TGAAGCAAGA GGTCCTTCCC AACGCATGGA AAACCAGAAC 
TATTCTGAAA TGTTACAAAA TAAGAAAAAA AGTCTCTCTC TCCAGCCCTC AACAGAGGAT 
TTGACTCCAG CTTGCAGTTC ATCTGATACT GCTCTGTTTG ATCAGTTACA CCTGCAGCAC 
AGCACTCCGT GTGCTTCTGT CTCTAGTGAG TGCTCAGTAA AACTGTTAGA AAGCAGAGAA 
GAAGTGCTGG GATTTGAAGA GCTGTCAAGA AGGGCAGTTA CAATGTCACA AAGACTCACT 
GAAGATGAAA ATGTAGTTCT ACCTATAAAC CCACATGTAG GAAGAGTGGA GAAGGAGGCT 
TCAGTTCAGG GGTCTAATCC ATTGAGTATT CAAAATGAAA AGCCAATTCA GAACTTCATT 
GAAACTGACA CTACAGAAGC TGTAGGAAAT GTATGTCAGC TAGCACAAGC AGAGCACATA 
TTAAAATCCT GTCCATTTCG GAGCCCTATT CCAATCTGGG AAACGGATAC TGGCTATGGT 
ATAATGGAAG AACCAGATCT CACTCTAGTG AGTAATAGTG ACATCAGCAT CACTGAAACA 
GATCTTGCAA ACTTAACCCT TGAAGACAGA GAAGACAATG AAGCACAGTT TTTTCAGGCA 
GGTGTCGTTC TGCCTACATC TTCAATGGAA ACCTCTGTTT GTGGAGCTGT ATCAGAGCCT 
TATGTGGACC AACCCACAGT GGCACCTTCA GCTACATCAG GTAGTTTACA AGAAGCATTT 
ATGACAAGAC AGACACTTAC AGAACGATCT TACCAGAGGC AGAGAGAGAT CTGGAATAAA 
ACTCGACTCC CTCAAACCAA AGTATCCAAA GAGAAGCTAC CTACAGGTTG CACAGGCAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168909.1
CDS	77..7459
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_017313420.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313420.1

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG AGTCATTTTC AGAACAGACA GAGCATCTAG AACAAGAATC TACCAATAAG 
CAGGAAGAGA CTGATCCTCT TAGTATTGCA GTCCCTTCAG TTATTTATCA GCAACAGCAT 
TCCTTAGGTG CTCATAACTC TCTGTTGCCC ATGGAGGAAG AAAGTACATC TGATCATACA 
CATGTTCAGC AGATCATGGA CAATGATGTA AATGAAGCAA ATTTGATACC TGACAAAAGA 
GACTTTCAGG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGAGCTCAG AATCTTCATC TGAAAAACAT 
GTTAAGGCAT TATCCACAGG CACAATCTGC TTCCCAGCAC TGACAGCCAA GTCACATTCT 
CCTAACCCAA GGCTGAACCA ACTTGATATT AACCTGGCTC ATGCAACAAC TGAGGGATCT 
GAACAGTCTT TTCAACAGCT TCGACCAGAA TTCTCTTCAC AGGAAAGCCA ACATGCTGAC 
CTACCCAGTA TTTATAGCAT TGAAGCAAGA GGTCCTTCCC AACGCATGGA AAACCAGAAC 
TATTCTGAAA TGTTACAAAA TAAGAAAAAA AGTCTCTCTC TCCAGCCCTC AACAGAGGAT 
TTGACTCCAG CTTGCAGTTC ATCTGATACT GCTCTGTTTG ATCAGTTACA CCTGCAGCAC 
AGCACTCCGT GTGCTTCTGT CTCTAGTGAG TGCTCAGTAA AACTGTTAGA AAGCAGAGAA 
GAAGTGCTGG GATTTGAAGA GCTGTCAAGA AGGGCAGTTA CAATGTCACA AAGACTCACT 
GAAGATGAAA ATGTAGTTCT ACCTATAAAC CCACATGTAG GAAGAGTGGA GAAGGAGGCT 
TCAGTTCAGG GGTCTAATCC ATTGAGTATT CAAAATGAAA AGCCAATTCA GAACTTCATT 
GAAACTGACA CTACAGAAGC TGTAGGAAAT GTATGTCAGC TAGCACAAGC AGAGCACATA 
TTAAAATCCT GTCCATTTCG GAGCCCTATT CCAATCTGGG AAACGGATAC TGGCTATGGT 
ATAATGGAAG AACCAGATCT CACTCTAGTG AGTAATAGTG ACATCAGCAT CACTGAAACA 
GATCTTGCAA ACTTAACCCT TGAAGACAGA GAAGACAATG AAGCACAGTT TTTTCAGGCA 
GGTGTCGTTC TGCCTACATC TTCAATGGAA ACCTCTGTTT GTGGAGCTGT ATCAGAGCCT 
TATGTGGACC AACCCACAGT GGCACCTTCA GCTACATCAG GTAGTTTACA AGAAGCATTT 
ATGACAAGAC AGACACTTAC AGAACGATCT TACCAGAGGC AGAGAGAGAT CTGGAATAAA 
ACTCGACTCC CTCAAACCAA AGTATCCAAA GAGAAGCTAC CTACAGGTTG CACAGGCAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_017313420.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168909.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X4, mRNA.

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CloneID	OMu82851
Clone ID Related Accession (Same CDS sequence)	XM_017313420.1 , XM_017313420.2
Accession Version	XM_017313420.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017313420.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG AGTCATTTTC AGAACAGACA GAGCATCTAG AACAAGAATC TACCAATAAG 
CAGGAAGAGA CTGATCCTCT TAGTATTGCA GTCCCTTCAG TTATTTATCA GCAACAGCAT 
TCCTTAGGTG CTCATAACTC TCTGTTGCCC ATGGAGGAAG AAAGTACATC TGATCATACA 
CATGTTCAGC AGATCATGGA CAATGATGTA AATGAAGCAA ATTTGATACC TGACAAAAGA 
GACTTTCAGG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGAGCTCAG AATCTTCATC TGAAAAACAT 
GTTAAGGCAT TATCCACAGG CACAATCTGC TTCCCAGCAC TGACAGCCAA GTCACATTCT 
CCTAACCCAA GGCTGAACCA ACTTGATATT AACCTGGCTC ATGCAACAAC TGAGGGATCT 
GAACAGTCTT TTCAACAGCT TCGACCAGAA TTCTCTTCAC AGGAAAGCCA ACATGCTGAC 
CTACCCAGTA TTTATAGCAT TGAAGCAAGA GGTCCTTCCC AACGCATGGA AAACCAGAAC 
TATTCTGAAA TGTTACAAAA TAAGAAAAAA AGTCTCTCTC TCCAGCCCTC AACAGAGGAT 
TTGACTCCAG CTTGCAGTTC ATCTGATACT GCTCTGTTTG ATCAGTTACA CCTGCAGCAC 
AGCACTCCGT GTGCTTCTGT CTCTAGTGAG TGCTCAGTAA AACTGTTAGA AAGCAGAGAA 
GAAGTGCTGG GATTTGAAGA GCTGTCAAGA AGGGCAGTTA CAATGTCACA AAGACTCACT 
GAAGATGAAA ATGTAGTTCT ACCTATAAAC CCACATGTAG GAAGAGTGGA GAAGGAGGCT 
TCAGTTCAGG GGTCTAATCC ATTGAGTATT CAAAATGAAA AGCCAATTCA GAACTTCATT 
GAAACTGACA CTACAGAAGC TGTAGGAAAT GTATGTCAGC TAGCACAAGC AGAGCACATA 
TTAAAATCCT GTCCATTTCG GAGCCCTATT CCAATCTGGG AAACGGATAC TGGCTATGGT 
ATAATGGAAG AACCAGATCT CACTCTAGTG AGTAATAGTG ACATCAGCAT CACTGAAACA 
GATCTTGCAA ACTTAACCCT TGAAGACAGA GAAGACAATG AAGCACAGTT TTTTCAGGCA 
GGTGTCGTTC TGCCTACATC TTCAATGGAA ACCTCTGTTT GTGGAGCTGT ATCAGAGCCT 
TATGTGGACC AACCCACAGT GGCACCTTCA GCTACATCAG GTAGTTTACA AGAAGCATTT 
ATGACAAGAC AGACACTTAC AGAACGATCT TACCAGAGGC AGAGAGAGAT CTGGAATAAA 
ACTCGACTCC CTCAAACCAA AGTATCCAAA GAGAAGCTAC CTACAGGTTG CACAGGCAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168909.1
CDS	107..7489
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_017313420.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313420.2

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG AGTCATTTTC AGAACAGACA GAGCATCTAG AACAAGAATC TACCAATAAG 
CAGGAAGAGA CTGATCCTCT TAGTATTGCA GTCCCTTCAG TTATTTATCA GCAACAGCAT 
TCCTTAGGTG CTCATAACTC TCTGTTGCCC ATGGAGGAAG AAAGTACATC TGATCATACA 
CATGTTCAGC AGATCATGGA CAATGATGTA AATGAAGCAA ATTTGATACC TGACAAAAGA 
GACTTTCAGG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGAGCTCAG AATCTTCATC TGAAAAACAT 
GTTAAGGCAT TATCCACAGG CACAATCTGC TTCCCAGCAC TGACAGCCAA GTCACATTCT 
CCTAACCCAA GGCTGAACCA ACTTGATATT AACCTGGCTC ATGCAACAAC TGAGGGATCT 
GAACAGTCTT TTCAACAGCT TCGACCAGAA TTCTCTTCAC AGGAAAGCCA ACATGCTGAC 
CTACCCAGTA TTTATAGCAT TGAAGCAAGA GGTCCTTCCC AACGCATGGA AAACCAGAAC 
TATTCTGAAA TGTTACAAAA TAAGAAAAAA AGTCTCTCTC TCCAGCCCTC AACAGAGGAT 
TTGACTCCAG CTTGCAGTTC ATCTGATACT GCTCTGTTTG ATCAGTTACA CCTGCAGCAC 
AGCACTCCGT GTGCTTCTGT CTCTAGTGAG TGCTCAGTAA AACTGTTAGA AAGCAGAGAA 
GAAGTGCTGG GATTTGAAGA GCTGTCAAGA AGGGCAGTTA CAATGTCACA AAGACTCACT 
GAAGATGAAA ATGTAGTTCT ACCTATAAAC CCACATGTAG GAAGAGTGGA GAAGGAGGCT 
TCAGTTCAGG GGTCTAATCC ATTGAGTATT CAAAATGAAA AGCCAATTCA GAACTTCATT 
GAAACTGACA CTACAGAAGC TGTAGGAAAT GTATGTCAGC TAGCACAAGC AGAGCACATA 
TTAAAATCCT GTCCATTTCG GAGCCCTATT CCAATCTGGG AAACGGATAC TGGCTATGGT 
ATAATGGAAG AACCAGATCT CACTCTAGTG AGTAATAGTG ACATCAGCAT CACTGAAACA 
GATCTTGCAA ACTTAACCCT TGAAGACAGA GAAGACAATG AAGCACAGTT TTTTCAGGCA 
GGTGTCGTTC TGCCTACATC TTCAATGGAA ACCTCTGTTT GTGGAGCTGT ATCAGAGCCT 
TATGTGGACC AACCCACAGT GGCACCTTCA GCTACATCAG GTAGTTTACA AGAAGCATTT 
ATGACAAGAC AGACACTTAC AGAACGATCT TACCAGAGGC AGAGAGAGAT CTGGAATAAA 
ACTCGACTCC CTCAAACCAA AGTATCCAAA GAGAAGCTAC CTACAGGTTG CACAGGCAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_017313421.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168910.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X7, mRNA.

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CloneID	OMu82852
Clone ID Related Accession (Same CDS sequence)	XM_017313421.2 , XM_017313421.1
Accession Version	XM_017313421.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017313421.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GAAAAGCAGA GTTTTATCAA 
GACCGAGACC CATTAAGGGT CTCAGTAAGC AGAGAACAAA GTTTCTTGGA GAGCCCACTG 
GCACATGATC CATTTGGTTG TCACCAACCA CCTGCCCAAG AGAACAGCAA AAGCCATGAC 
GACAATGCTG AAGCAGTTAA AGTCAAGAAA TCTGATGTTG AGGATCATGC AGTATTGAGT 
CATGCTGTCT CAAAAGAAGA AGCGTGTACA AACCTGGGTC CACTTGGAAA GCCAGACGAT 
GAGGCTGAAA CACAAGAGAT TTCTCAGGAG CCATTGTCTT CAGTAACTGT TTCTACTGGA 
AGCTTCTTAA GTTATGAAAT CACAGATTTG AGCCTCACAG ATCCAGAGTC ATTTTCAGAA 
CAGACAGAGC ATCTAGAACA AGAATCTACC AATAAGCAGG AAGAGACTGA TCCTCTTAGT 
ATTGCAGTCC CTTCAGTTAT TTATCAGCAA CAGCATTCCT TAGGTGCTCA TAACTCTCTG 
TTGCCCATGG AGGAAGAAAG TACATCTGAT CATACACATG TTCAGCAGAT CATGGACAAT 
GATGTAAATG AAGCAAATTT GATACCTGAC AAAAGAGACT TTCAGGTTCC AGCTGTGGAC 
CTTGACTTTC GAGAATTGGA ACACATATTT CCACACTTGC ATCGTCAGCT CTTTAAACCC 
TTAGAACCAC ATCTAGATTT TGACTTATCA TCCCCTGGGA CATCTCAAGA AGACAGCGAC 
TTTTACCAGA GCTCAGAATC TTCATCTGAA AAACATGTTA AGGCATTATC CACAGGCACA 
ATCTGCTTCC CAGCACTGAC AGCCAAGTCA CATTCTCCTA ACCCAAGGCT GAACCAACTT 
GATATTAACC TGGCTCATGC AACAACTGAG GGATCTGAAC AGTCTTTTCA ACAGCTTCGA 
CCAGAATTCT CTTCACAGGA AAGCCAACAT GCTGACCTAC CCAGTATTTA TAGCATTGAA 
GCAAGAGGTC CTTCCCAACG CATGGAAAAC CAGAACTATT CTGAAATGTT ACAAAATAAG 
AAAAAAAGTC TCTCTCTCCA GCCCTCAACA GAGGATTTGA CTCCAGCTTG CAGTTCATCT 
GATACTGCTC TGTTTGATCA GTTACACCTG CAGCACAGCA CTCCGTGTGC TTCTGTCTCT 
AGTGAGTGCT CAGTAAAACT GTTAGAAAGC AGAGAAGAAG TGCTGGGATT TGAAGAGCTG 
TCAAGAAGGG CAGTTACAAT GTCACAAAGA CTCACTGAAG ATGAAAATGT AGTTCTACCT 
ATAAACCCAC ATGTAGGAAG AGTGGAGAAG GAGGCTTCAG TTCAGGGGTC TAATCCATTG 
AGTATTCAAA ATGAAAAGCC AATTCAGAAC TTCATTGAAA CTGACACTAC AGAAGCTGTA 
GGAAATGTAT GTCAGCTAGC ACAAGCAGAG CACATATTAA AATCCTGTCC ATTTCGGAGC 
CCTATTCCAA TCTGGGAAAC GGATACTGGC TATGGTATAA TGGAAGAACC AGATCTCACT 
CTAGTGAGTA ATAGTGACAT CAGCATCACT GAAACAGATC TTGCAAACTT AACCCTTGAA 
GACAGAGAAG ACAATGAAGC ACAGTTTTTT CAGGCAGGTG TCGTTCTGCC TACATCTTCA 
ATGGAAACCT CTGTTTGTGG AGCTGTATCA GAGCCTTATG TGGACCAACC CACAGTGGCA 
CCTTCAGCTA CATCAGGTAG TTTACAAGAA GCATTTATGA CAAGACAGAC ACTTACAGAA 
CGATCTTACC AGAGGCAGAG AGAGATCTGG AATAAAACTC GACTCCCTCA AACCAAAGTA 
TCCAAAGAGA AGCTACCTAC AGGTTGCACA GGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168910.1
CDS	107..7345
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_017313421.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313421.2

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GAAAAGCAGA GTTTTATCAA 
GACCGAGACC CATTAAGGGT CTCAGTAAGC AGAGAACAAA GTTTCTTGGA GAGCCCACTG 
GCACATGATC CATTTGGTTG TCACCAACCA CCTGCCCAAG AGAACAGCAA AAGCCATGAC 
GACAATGCTG AAGCAGTTAA AGTCAAGAAA TCTGATGTTG AGGATCATGC AGTATTGAGT 
CATGCTGTCT CAAAAGAAGA AGCGTGTACA AACCTGGGTC CACTTGGAAA GCCAGACGAT 
GAGGCTGAAA CACAAGAGAT TTCTCAGGAG CCATTGTCTT CAGTAACTGT TTCTACTGGA 
AGCTTCTTAA GTTATGAAAT CACAGATTTG AGCCTCACAG ATCCAGAGTC ATTTTCAGAA 
CAGACAGAGC ATCTAGAACA AGAATCTACC AATAAGCAGG AAGAGACTGA TCCTCTTAGT 
ATTGCAGTCC CTTCAGTTAT TTATCAGCAA CAGCATTCCT TAGGTGCTCA TAACTCTCTG 
TTGCCCATGG AGGAAGAAAG TACATCTGAT CATACACATG TTCAGCAGAT CATGGACAAT 
GATGTAAATG AAGCAAATTT GATACCTGAC AAAAGAGACT TTCAGGTTCC AGCTGTGGAC 
CTTGACTTTC GAGAATTGGA ACACATATTT CCACACTTGC ATCGTCAGCT CTTTAAACCC 
TTAGAACCAC ATCTAGATTT TGACTTATCA TCCCCTGGGA CATCTCAAGA AGACAGCGAC 
TTTTACCAGA GCTCAGAATC TTCATCTGAA AAACATGTTA AGGCATTATC CACAGGCACA 
ATCTGCTTCC CAGCACTGAC AGCCAAGTCA CATTCTCCTA ACCCAAGGCT GAACCAACTT 
GATATTAACC TGGCTCATGC AACAACTGAG GGATCTGAAC AGTCTTTTCA ACAGCTTCGA 
CCAGAATTCT CTTCACAGGA AAGCCAACAT GCTGACCTAC CCAGTATTTA TAGCATTGAA 
GCAAGAGGTC CTTCCCAACG CATGGAAAAC CAGAACTATT CTGAAATGTT ACAAAATAAG 
AAAAAAAGTC TCTCTCTCCA GCCCTCAACA GAGGATTTGA CTCCAGCTTG CAGTTCATCT 
GATACTGCTC TGTTTGATCA GTTACACCTG CAGCACAGCA CTCCGTGTGC TTCTGTCTCT 
AGTGAGTGCT CAGTAAAACT GTTAGAAAGC AGAGAAGAAG TGCTGGGATT TGAAGAGCTG 
TCAAGAAGGG CAGTTACAAT GTCACAAAGA CTCACTGAAG ATGAAAATGT AGTTCTACCT 
ATAAACCCAC ATGTAGGAAG AGTGGAGAAG GAGGCTTCAG TTCAGGGGTC TAATCCATTG 
AGTATTCAAA ATGAAAAGCC AATTCAGAAC TTCATTGAAA CTGACACTAC AGAAGCTGTA 
GGAAATGTAT GTCAGCTAGC ACAAGCAGAG CACATATTAA AATCCTGTCC ATTTCGGAGC 
CCTATTCCAA TCTGGGAAAC GGATACTGGC TATGGTATAA TGGAAGAACC AGATCTCACT 
CTAGTGAGTA ATAGTGACAT CAGCATCACT GAAACAGATC TTGCAAACTT AACCCTTGAA 
GACAGAGAAG ACAATGAAGC ACAGTTTTTT CAGGCAGGTG TCGTTCTGCC TACATCTTCA 
ATGGAAACCT CTGTTTGTGG AGCTGTATCA GAGCCTTATG TGGACCAACC CACAGTGGCA 
CCTTCAGCTA CATCAGGTAG TTTACAAGAA GCATTTATGA CAAGACAGAC ACTTACAGAA 
CGATCTTACC AGAGGCAGAG AGAGATCTGG AATAAAACTC GACTCCCTCA AACCAAAGTA 
TCCAAAGAGA AGCTACCTAC AGGTTGCACA GGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_017313421.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168910.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X7, mRNA.

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CloneID	OMu82852
Clone ID Related Accession (Same CDS sequence)	XM_017313421.2 , XM_017313421.1
Accession Version	XM_017313421.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GAAAAGCAGA GTTTTATCAA 
GACCGAGACC CATTAAGGGT CTCAGTAAGC AGAGAACAAA GTTTCTTGGA GAGCCCACTG 
GCACATGATC CATTTGGTTG TCACCAACCA CCTGCCCAAG AGAACAGCAA AAGCCATGAC 
GACAATGCTG AAGCAGTTAA AGTCAAGAAA TCTGATGTTG AGGATCATGC AGTATTGAGT 
CATGCTGTCT CAAAAGAAGA AGCGTGTACA AACCTGGGTC CACTTGGAAA GCCAGACGAT 
GAGGCTGAAA CACAAGAGAT TTCTCAGGAG CCATTGTCTT CAGTAACTGT TTCTACTGGA 
AGCTTCTTAA GTTATGAAAT CACAGATTTG AGCCTCACAG ATCCAGAGTC ATTTTCAGAA 
CAGACAGAGC ATCTAGAACA AGAATCTACC AATAAGCAGG AAGAGACTGA TCCTCTTAGT 
ATTGCAGTCC CTTCAGTTAT TTATCAGCAA CAGCATTCCT TAGGTGCTCA TAACTCTCTG 
TTGCCCATGG AGGAAGAAAG TACATCTGAT CATACACATG TTCAGCAGAT CATGGACAAT 
GATGTAAATG AAGCAAATTT GATACCTGAC AAAAGAGACT TTCAGGTTCC AGCTGTGGAC 
CTTGACTTTC GAGAATTGGA ACACATATTT CCACACTTGC ATCGTCAGCT CTTTAAACCC 
TTAGAACCAC ATCTAGATTT TGACTTATCA TCCCCTGGGA CATCTCAAGA AGACAGCGAC 
TTTTACCAGA GCTCAGAATC TTCATCTGAA AAACATGTTA AGGCATTATC CACAGGCACA 
ATCTGCTTCC CAGCACTGAC AGCCAAGTCA CATTCTCCTA ACCCAAGGCT GAACCAACTT 
GATATTAACC TGGCTCATGC AACAACTGAG GGATCTGAAC AGTCTTTTCA ACAGCTTCGA 
CCAGAATTCT CTTCACAGGA AAGCCAACAT GCTGACCTAC CCAGTATTTA TAGCATTGAA 
GCAAGAGGTC CTTCCCAACG CATGGAAAAC CAGAACTATT CTGAAATGTT ACAAAATAAG 
AAAAAAAGTC TCTCTCTCCA GCCCTCAACA GAGGATTTGA CTCCAGCTTG CAGTTCATCT 
GATACTGCTC TGTTTGATCA GTTACACCTG CAGCACAGCA CTCCGTGTGC TTCTGTCTCT 
AGTGAGTGCT CAGTAAAACT GTTAGAAAGC AGAGAAGAAG TGCTGGGATT TGAAGAGCTG 
TCAAGAAGGG CAGTTACAAT GTCACAAAGA CTCACTGAAG ATGAAAATGT AGTTCTACCT 
ATAAACCCAC ATGTAGGAAG AGTGGAGAAG GAGGCTTCAG TTCAGGGGTC TAATCCATTG 
AGTATTCAAA ATGAAAAGCC AATTCAGAAC TTCATTGAAA CTGACACTAC AGAAGCTGTA 
GGAAATGTAT GTCAGCTAGC ACAAGCAGAG CACATATTAA AATCCTGTCC ATTTCGGAGC 
CCTATTCCAA TCTGGGAAAC GGATACTGGC TATGGTATAA TGGAAGAACC AGATCTCACT 
CTAGTGAGTA ATAGTGACAT CAGCATCACT GAAACAGATC TTGCAAACTT AACCCTTGAA 
GACAGAGAAG ACAATGAAGC ACAGTTTTTT CAGGCAGGTG TCGTTCTGCC TACATCTTCA 
ATGGAAACCT CTGTTTGTGG AGCTGTATCA GAGCCTTATG TGGACCAACC CACAGTGGCA 
CCTTCAGCTA CATCAGGTAG TTTACAAGAA GCATTTATGA CAAGACAGAC ACTTACAGAA 
CGATCTTACC AGAGGCAGAG AGAGATCTGG AATAAAACTC GACTCCCTCA AACCAAAGTA 
TCCAAAGAGA AGCTACCTAC AGGTTGCACA GGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168910.1
CDS	77..7315
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_017313421.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313421.1

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GAAAAGCAGA GTTTTATCAA 
GACCGAGACC CATTAAGGGT CTCAGTAAGC AGAGAACAAA GTTTCTTGGA GAGCCCACTG 
GCACATGATC CATTTGGTTG TCACCAACCA CCTGCCCAAG AGAACAGCAA AAGCCATGAC 
GACAATGCTG AAGCAGTTAA AGTCAAGAAA TCTGATGTTG AGGATCATGC AGTATTGAGT 
CATGCTGTCT CAAAAGAAGA AGCGTGTACA AACCTGGGTC CACTTGGAAA GCCAGACGAT 
GAGGCTGAAA CACAAGAGAT TTCTCAGGAG CCATTGTCTT CAGTAACTGT TTCTACTGGA 
AGCTTCTTAA GTTATGAAAT CACAGATTTG AGCCTCACAG ATCCAGAGTC ATTTTCAGAA 
CAGACAGAGC ATCTAGAACA AGAATCTACC AATAAGCAGG AAGAGACTGA TCCTCTTAGT 
ATTGCAGTCC CTTCAGTTAT TTATCAGCAA CAGCATTCCT TAGGTGCTCA TAACTCTCTG 
TTGCCCATGG AGGAAGAAAG TACATCTGAT CATACACATG TTCAGCAGAT CATGGACAAT 
GATGTAAATG AAGCAAATTT GATACCTGAC AAAAGAGACT TTCAGGTTCC AGCTGTGGAC 
CTTGACTTTC GAGAATTGGA ACACATATTT CCACACTTGC ATCGTCAGCT CTTTAAACCC 
TTAGAACCAC ATCTAGATTT TGACTTATCA TCCCCTGGGA CATCTCAAGA AGACAGCGAC 
TTTTACCAGA GCTCAGAATC TTCATCTGAA AAACATGTTA AGGCATTATC CACAGGCACA 
ATCTGCTTCC CAGCACTGAC AGCCAAGTCA CATTCTCCTA ACCCAAGGCT GAACCAACTT 
GATATTAACC TGGCTCATGC AACAACTGAG GGATCTGAAC AGTCTTTTCA ACAGCTTCGA 
CCAGAATTCT CTTCACAGGA AAGCCAACAT GCTGACCTAC CCAGTATTTA TAGCATTGAA 
GCAAGAGGTC CTTCCCAACG CATGGAAAAC CAGAACTATT CTGAAATGTT ACAAAATAAG 
AAAAAAAGTC TCTCTCTCCA GCCCTCAACA GAGGATTTGA CTCCAGCTTG CAGTTCATCT 
GATACTGCTC TGTTTGATCA GTTACACCTG CAGCACAGCA CTCCGTGTGC TTCTGTCTCT 
AGTGAGTGCT CAGTAAAACT GTTAGAAAGC AGAGAAGAAG TGCTGGGATT TGAAGAGCTG 
TCAAGAAGGG CAGTTACAAT GTCACAAAGA CTCACTGAAG ATGAAAATGT AGTTCTACCT 
ATAAACCCAC ATGTAGGAAG AGTGGAGAAG GAGGCTTCAG TTCAGGGGTC TAATCCATTG 
AGTATTCAAA ATGAAAAGCC AATTCAGAAC TTCATTGAAA CTGACACTAC AGAAGCTGTA 
GGAAATGTAT GTCAGCTAGC ACAAGCAGAG CACATATTAA AATCCTGTCC ATTTCGGAGC 
CCTATTCCAA TCTGGGAAAC GGATACTGGC TATGGTATAA TGGAAGAACC AGATCTCACT 
CTAGTGAGTA ATAGTGACAT CAGCATCACT GAAACAGATC TTGCAAACTT AACCCTTGAA 
GACAGAGAAG ACAATGAAGC ACAGTTTTTT CAGGCAGGTG TCGTTCTGCC TACATCTTCA 
ATGGAAACCT CTGTTTGTGG AGCTGTATCA GAGCCTTATG TGGACCAACC CACAGTGGCA 
CCTTCAGCTA CATCAGGTAG TTTACAAGAA GCATTTATGA CAAGACAGAC ACTTACAGAA 
CGATCTTACC AGAGGCAGAG AGAGATCTGG AATAAAACTC GACTCCCTCA AACCAAAGTA 
TCCAAAGAGA AGCTACCTAC AGGTTGCACA GGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_017313422.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168911.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X8, mRNA.

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CloneID	OMu82853
Clone ID Related Accession (Same CDS sequence)	XM_017313422.2 , XM_017313422.1
Accession Version	XM_017313422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017313422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGTAAAC AAGGAAAAGC AGAGTTTTAT CAAGACCGAG ACCCATTAAG GGTCTCAGTA 
AGCAGAGAAC AAAGTTTCTT GGAGAGCCCA CTGGCACATG ATCCATTTGG TTGTCACCAA 
CCACCTGCCC AAGAGAACAG CAAAAGCCAT GACGACAATG CTGAAGCAGT TAAAGTCAAG 
AAATCTGATG TTGAGGATCA TGCAGTATTG AGTCATGCTG TCTCAAAAGA AGAAGCGTGT 
ACAAACCTGG GTCCACTTGG AAAGCCAGAC GATGAGGCTG AAACACAAGA GATTTCTCAG 
GAGCCATTGT CTTCAGTAAC TGTTTCTACT GGAAGCTTCT TAAGTTATGA AATCACAGAT 
TTGAGCCTCA CAGATCCAGT TCCAGCTGTG GACCTTGACT TTCGAGAATT GGAACACATA 
TTTCCACACT TGCATCGTCA GCTCTTTAAA CCCTTAGAAC CACATCTAGA TTTTGACTTA 
TCATCCCCTG GGACATCTCA AGAAGACAGC GACTTTTACC AGCAGAGCTC AGAATCTTCA 
TCTGAAAAAC ATGTTAAGGC ATTATCCACA GGCACAATCT GCTTCCCAGC ACTGACAGCC 
AAGTCACATT CTCCTAACCC AAGGCTGAAC CAACTTGATA TTAACCTGGC TCATGCAACA 
ACTGAGGGAT CTGAACAGTC TTTTCAACAG CTTCGACCAG AATTCTCTTC ACAGGAAAGC 
CAACATGCTG ACCTACCCAG TATTTATAGC ATTGAAGCAA GAGGTCCTTC CCAACGCATG 
GAAAACCAGA ACTATTCTGA AATGTTACAA AATAAGAAAA AAAGTCTCTC TCTCCAGCCC 
TCAACAGAGG ATTTGACTCC AGCTTGCAGT TCATCTGATA CTGCTCTGTT TGATCAGTTA 
CACCTGCAGC ACAGCACTCC GTGTGCTTCT GTCTCTAGTG AGTGCTCAGT AAAACTGTTA 
GAAAGCAGAG AAGAAGTGCT GGGATTTGAA GAGCTGTCAA GAAGGGCAGT TACAATGTCA 
CAAAGACTCA CTGAAGATGA AAATGTAGTT CTACCTATAA ACCCACATGT AGGAAGAGTG 
GAGAAGGAGG CTTCAGTTCA GGGGTCTAAT CCATTGAGTA TTCAAAATGA AAAGCCAATT 
CAGAACTTCA TTGAAACTGA CACTACAGAA GCTGTAGGAA ATGTATGTCA GCTAGCACAA 
GCAGAGCACA TATTAAAATC CTGTCCATTT CGGAGCCCTA TTCCAATCTG GGAAACGGAT 
ACTGGCTATG GTATAATGGA AGAACCAGAT CTCACTCTAG TGAGTAATAG TGACATCAGC 
ATCACTGAAA CAGATCTTGC AAACTTAACC CTTGAAGACA GAGAAGACAA TGAAGCACAG 
TTTTTTCAGG CAGGTGTCGT TCTGCCTACA TCTTCAATGG AAACCTCTGT TTGTGGAGCT 
GTATCAGAGC CTTATGTGGA CCAACCCACA GTGGCACCTT CAGCTACATC AGGTAGTTTA 
CAAGAAGCAT TTATGACAAG ACAGACACTT ACAGAACGAT CTTACCAGAG GCAGAGAGAG 
ATCTGGAATA AAACTCGACT CCCTCAAACC AAAGTATCCA AAGAGAAGCT ACCTACAGGT 
TGCACAGGCA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168911.1
CDS	107..7261
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_017313422.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313422.2

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGTAAAC AAGGAAAAGC AGAGTTTTAT CAAGACCGAG ACCCATTAAG GGTCTCAGTA 
AGCAGAGAAC AAAGTTTCTT GGAGAGCCCA CTGGCACATG ATCCATTTGG TTGTCACCAA 
CCACCTGCCC AAGAGAACAG CAAAAGCCAT GACGACAATG CTGAAGCAGT TAAAGTCAAG 
AAATCTGATG TTGAGGATCA TGCAGTATTG AGTCATGCTG TCTCAAAAGA AGAAGCGTGT 
ACAAACCTGG GTCCACTTGG AAAGCCAGAC GATGAGGCTG AAACACAAGA GATTTCTCAG 
GAGCCATTGT CTTCAGTAAC TGTTTCTACT GGAAGCTTCT TAAGTTATGA AATCACAGAT 
TTGAGCCTCA CAGATCCAGT TCCAGCTGTG GACCTTGACT TTCGAGAATT GGAACACATA 
TTTCCACACT TGCATCGTCA GCTCTTTAAA CCCTTAGAAC CACATCTAGA TTTTGACTTA 
TCATCCCCTG GGACATCTCA AGAAGACAGC GACTTTTACC AGCAGAGCTC AGAATCTTCA 
TCTGAAAAAC ATGTTAAGGC ATTATCCACA GGCACAATCT GCTTCCCAGC ACTGACAGCC 
AAGTCACATT CTCCTAACCC AAGGCTGAAC CAACTTGATA TTAACCTGGC TCATGCAACA 
ACTGAGGGAT CTGAACAGTC TTTTCAACAG CTTCGACCAG AATTCTCTTC ACAGGAAAGC 
CAACATGCTG ACCTACCCAG TATTTATAGC ATTGAAGCAA GAGGTCCTTC CCAACGCATG 
GAAAACCAGA ACTATTCTGA AATGTTACAA AATAAGAAAA AAAGTCTCTC TCTCCAGCCC 
TCAACAGAGG ATTTGACTCC AGCTTGCAGT TCATCTGATA CTGCTCTGTT TGATCAGTTA 
CACCTGCAGC ACAGCACTCC GTGTGCTTCT GTCTCTAGTG AGTGCTCAGT AAAACTGTTA 
GAAAGCAGAG AAGAAGTGCT GGGATTTGAA GAGCTGTCAA GAAGGGCAGT TACAATGTCA 
CAAAGACTCA CTGAAGATGA AAATGTAGTT CTACCTATAA ACCCACATGT AGGAAGAGTG 
GAGAAGGAGG CTTCAGTTCA GGGGTCTAAT CCATTGAGTA TTCAAAATGA AAAGCCAATT 
CAGAACTTCA TTGAAACTGA CACTACAGAA GCTGTAGGAA ATGTATGTCA GCTAGCACAA 
GCAGAGCACA TATTAAAATC CTGTCCATTT CGGAGCCCTA TTCCAATCTG GGAAACGGAT 
ACTGGCTATG GTATAATGGA AGAACCAGAT CTCACTCTAG TGAGTAATAG TGACATCAGC 
ATCACTGAAA CAGATCTTGC AAACTTAACC CTTGAAGACA GAGAAGACAA TGAAGCACAG 
TTTTTTCAGG CAGGTGTCGT TCTGCCTACA TCTTCAATGG AAACCTCTGT TTGTGGAGCT 
GTATCAGAGC CTTATGTGGA CCAACCCACA GTGGCACCTT CAGCTACATC AGGTAGTTTA 
CAAGAAGCAT TTATGACAAG ACAGACACTT ACAGAACGAT CTTACCAGAG GCAGAGAGAG 
ATCTGGAATA AAACTCGACT CCCTCAAACC AAAGTATCCA AAGAGAAGCT ACCTACAGGT 
TGCACAGGCA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_017313422.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168911.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X8, mRNA.

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CloneID	OMu82853
Clone ID Related Accession (Same CDS sequence)	XM_017313422.2 , XM_017313422.1
Accession Version	XM_017313422.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGTAAAC AAGGAAAAGC AGAGTTTTAT CAAGACCGAG ACCCATTAAG GGTCTCAGTA 
AGCAGAGAAC AAAGTTTCTT GGAGAGCCCA CTGGCACATG ATCCATTTGG TTGTCACCAA 
CCACCTGCCC AAGAGAACAG CAAAAGCCAT GACGACAATG CTGAAGCAGT TAAAGTCAAG 
AAATCTGATG TTGAGGATCA TGCAGTATTG AGTCATGCTG TCTCAAAAGA AGAAGCGTGT 
ACAAACCTGG GTCCACTTGG AAAGCCAGAC GATGAGGCTG AAACACAAGA GATTTCTCAG 
GAGCCATTGT CTTCAGTAAC TGTTTCTACT GGAAGCTTCT TAAGTTATGA AATCACAGAT 
TTGAGCCTCA CAGATCCAGT TCCAGCTGTG GACCTTGACT TTCGAGAATT GGAACACATA 
TTTCCACACT TGCATCGTCA GCTCTTTAAA CCCTTAGAAC CACATCTAGA TTTTGACTTA 
TCATCCCCTG GGACATCTCA AGAAGACAGC GACTTTTACC AGCAGAGCTC AGAATCTTCA 
TCTGAAAAAC ATGTTAAGGC ATTATCCACA GGCACAATCT GCTTCCCAGC ACTGACAGCC 
AAGTCACATT CTCCTAACCC AAGGCTGAAC CAACTTGATA TTAACCTGGC TCATGCAACA 
ACTGAGGGAT CTGAACAGTC TTTTCAACAG CTTCGACCAG AATTCTCTTC ACAGGAAAGC 
CAACATGCTG ACCTACCCAG TATTTATAGC ATTGAAGCAA GAGGTCCTTC CCAACGCATG 
GAAAACCAGA ACTATTCTGA AATGTTACAA AATAAGAAAA AAAGTCTCTC TCTCCAGCCC 
TCAACAGAGG ATTTGACTCC AGCTTGCAGT TCATCTGATA CTGCTCTGTT TGATCAGTTA 
CACCTGCAGC ACAGCACTCC GTGTGCTTCT GTCTCTAGTG AGTGCTCAGT AAAACTGTTA 
GAAAGCAGAG AAGAAGTGCT GGGATTTGAA GAGCTGTCAA GAAGGGCAGT TACAATGTCA 
CAAAGACTCA CTGAAGATGA AAATGTAGTT CTACCTATAA ACCCACATGT AGGAAGAGTG 
GAGAAGGAGG CTTCAGTTCA GGGGTCTAAT CCATTGAGTA TTCAAAATGA AAAGCCAATT 
CAGAACTTCA TTGAAACTGA CACTACAGAA GCTGTAGGAA ATGTATGTCA GCTAGCACAA 
GCAGAGCACA TATTAAAATC CTGTCCATTT CGGAGCCCTA TTCCAATCTG GGAAACGGAT 
ACTGGCTATG GTATAATGGA AGAACCAGAT CTCACTCTAG TGAGTAATAG TGACATCAGC 
ATCACTGAAA CAGATCTTGC AAACTTAACC CTTGAAGACA GAGAAGACAA TGAAGCACAG 
TTTTTTCAGG CAGGTGTCGT TCTGCCTACA TCTTCAATGG AAACCTCTGT TTGTGGAGCT 
GTATCAGAGC CTTATGTGGA CCAACCCACA GTGGCACCTT CAGCTACATC AGGTAGTTTA 
CAAGAAGCAT TTATGACAAG ACAGACACTT ACAGAACGAT CTTACCAGAG GCAGAGAGAG 
ATCTGGAATA AAACTCGACT CCCTCAAACC AAAGTATCCA AAGAGAAGCT ACCTACAGGT 
TGCACAGGCA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168911.1
CDS	78..7232
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_017313422.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313422.1

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGTAAAC AAGGAAAAGC AGAGTTTTAT CAAGACCGAG ACCCATTAAG GGTCTCAGTA 
AGCAGAGAAC AAAGTTTCTT GGAGAGCCCA CTGGCACATG ATCCATTTGG TTGTCACCAA 
CCACCTGCCC AAGAGAACAG CAAAAGCCAT GACGACAATG CTGAAGCAGT TAAAGTCAAG 
AAATCTGATG TTGAGGATCA TGCAGTATTG AGTCATGCTG TCTCAAAAGA AGAAGCGTGT 
ACAAACCTGG GTCCACTTGG AAAGCCAGAC GATGAGGCTG AAACACAAGA GATTTCTCAG 
GAGCCATTGT CTTCAGTAAC TGTTTCTACT GGAAGCTTCT TAAGTTATGA AATCACAGAT 
TTGAGCCTCA CAGATCCAGT TCCAGCTGTG GACCTTGACT TTCGAGAATT GGAACACATA 
TTTCCACACT TGCATCGTCA GCTCTTTAAA CCCTTAGAAC CACATCTAGA TTTTGACTTA 
TCATCCCCTG GGACATCTCA AGAAGACAGC GACTTTTACC AGCAGAGCTC AGAATCTTCA 
TCTGAAAAAC ATGTTAAGGC ATTATCCACA GGCACAATCT GCTTCCCAGC ACTGACAGCC 
AAGTCACATT CTCCTAACCC AAGGCTGAAC CAACTTGATA TTAACCTGGC TCATGCAACA 
ACTGAGGGAT CTGAACAGTC TTTTCAACAG CTTCGACCAG AATTCTCTTC ACAGGAAAGC 
CAACATGCTG ACCTACCCAG TATTTATAGC ATTGAAGCAA GAGGTCCTTC CCAACGCATG 
GAAAACCAGA ACTATTCTGA AATGTTACAA AATAAGAAAA AAAGTCTCTC TCTCCAGCCC 
TCAACAGAGG ATTTGACTCC AGCTTGCAGT TCATCTGATA CTGCTCTGTT TGATCAGTTA 
CACCTGCAGC ACAGCACTCC GTGTGCTTCT GTCTCTAGTG AGTGCTCAGT AAAACTGTTA 
GAAAGCAGAG AAGAAGTGCT GGGATTTGAA GAGCTGTCAA GAAGGGCAGT TACAATGTCA 
CAAAGACTCA CTGAAGATGA AAATGTAGTT CTACCTATAA ACCCACATGT AGGAAGAGTG 
GAGAAGGAGG CTTCAGTTCA GGGGTCTAAT CCATTGAGTA TTCAAAATGA AAAGCCAATT 
CAGAACTTCA TTGAAACTGA CACTACAGAA GCTGTAGGAA ATGTATGTCA GCTAGCACAA 
GCAGAGCACA TATTAAAATC CTGTCCATTT CGGAGCCCTA TTCCAATCTG GGAAACGGAT 
ACTGGCTATG GTATAATGGA AGAACCAGAT CTCACTCTAG TGAGTAATAG TGACATCAGC 
ATCACTGAAA CAGATCTTGC AAACTTAACC CTTGAAGACA GAGAAGACAA TGAAGCACAG 
TTTTTTCAGG CAGGTGTCGT TCTGCCTACA TCTTCAATGG AAACCTCTGT TTGTGGAGCT 
GTATCAGAGC CTTATGTGGA CCAACCCACA GTGGCACCTT CAGCTACATC AGGTAGTTTA 
CAAGAAGCAT TTATGACAAG ACAGACACTT ACAGAACGAT CTTACCAGAG GCAGAGAGAG 
ATCTGGAATA AAACTCGACT CCCTCAAACC AAAGTATCCA AAGAGAAGCT ACCTACAGGT 
TGCACAGGCA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_017313423.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168912.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X10, mRNA.

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CloneID	OMu82854
Clone ID Related Accession (Same CDS sequence)	XM_017313423.1 , XM_017313423.2
Accession Version	XM_017313423.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGCA AGGAATCCCA CAGATGAGAA TGACTTGGAA 
ATGCAGAAGA GAGAACAGCT CGGAGGGTGG TTTCCTCACA CCCAAGGCCT TACCTGGGGA 
GACGCAGGTC AGGGGAGCGC TAATGGTGAG CAGCCACGTG CAGATGTCCA TGCTGAGCAC 
AATGGTGAGA GTCTGGCCAA AGAACTGAGT GGTAGAGCCT CAAAGCCACC TGTCTCCAAA 
GTTAAATGTG TCTTGGACTT AAACCAGCAT GAGCTTAGTA CTATACAGGA AGTAGAGTCA 
CCAGCAAGTG GCAGGATTTC CATGCCAGGT AAACAAGGAA AAGCAGAGTT TTATCAAGAC 
CGAGACCCAT TAAGGGTCTC AGTAAGCAGA GAACAAAGTT TCTTGGAGAG CCCACTGGCA 
CATGATCCAT TTGGTTGTCA CCAACCACCT GCCCAAGAGA ACAGCAAAAG CCATGACGAC 
AATGCTGAAG CAGTTAAAGT CAAGAAATCT GATGTTGAGG ATCATGCAGT ATTGAGTCAT 
GCTGTCTCAA AAGAAGAAGC GTGTACAAAC CTGGGTCCAC TTGGAAAGCC AGACGATGAG 
GCTGAAACAC AAGAGATTTC TCAGGAGCCA TTGTCTTCAG TAACTGTTTC TACTGGAAGC 
TTCTTAAGTT ATGAAATCAC AGATTTGAGC CTCACAGATC CAGAGTCATT TTCAGAACAG 
ACAGAGCATC TAGAACAAGA ATCTACCAAT AAGCAGGAAG AGACTGATCC TCTTAGTATT 
GCAGTCCCTT CAGTTATTTA TCAGCAACAG CATTCCTTAG GTGCTCATAA CTCTCTGTTG 
CCCATGGAGG AAGAAAGTAC ATCTGATCAT ACACATGTTC AGCAGATCAT GGACAATGAT 
GTAAATGAAG CAAATTTGAT ACCTGACAAA AGAGACTTTC AGGTTCCAGC TGTGGACCTT 
GACTTTCGAG AATTGGAACA CATATTTCCA CACTTGCATC GTCAGCTCTT TAAACCCTTA 
GAACCACATC TAGATTTTGA CTTATCATCC CCTGGGACAT CTCAAGAAGA CAGCGACTTT 
TACCAGCAGA GCTCAGAATC TTCATCTGAA AAACATGTTA AGGCATTATC CACAGGCACA 
ATCTGCTTCC CAGCACTGAC AGCCAAGTCA CATTCTCCTA ACCCAAGGCT GAACCAACTT 
GATATTAACC TGGCTCATGC AACAACTGAG GGATCTGAAC AGTCTTTTCA ACAGCTTCGA 
CCAGAATTCT CTTCACAGGA AAGCCAACAT GCTGACCTAC CCAGTATTTA TAGCATTGAA 
GCAAGAGGTC CTTCCCAACG CATGGAAAAC CAGAACTATT CTGAAATGTT ACAAAATAAG 
AAAAAAAGTC TCTCTCTCCA GCCCTCAACA GAGGATTTGA CTCCAGCTTG CAGTTCATCT 
GATACTGCTC TGTTTGATCA GTTACACCTG CAGCACAGCA CTCCGTGTGC TTCTGTCTCT 
AGTGAGTGCT CAGTAAAACT GTTAGAAAGC AGAGAAGAAG TGCTGGGATT TGAAGAGCTG 
TCAAGAAGGG CAGTTACAAT GTCACAAAGA CTCACTGAAG ATGAAAATGT AGTTCTACCT 
ATAAACCCAC ATGTAGGAAG AGTGGAGAAG GAGGCTTCAG TTCAGGGGTC TAATCCATTG 
AGTATTCAAA ATGAAAAGCC AATTCAGAAC TTCATTGAAA CTGACACTAC AGAAGCTGTA 
GGAAATGTAT GTCAGCTAGC ACAAGCAGAG CACATATTAA AATCCTGTCC ATTTCGGAGC 
CCTATTCCAA TCTGGGAAAC GGATACTGGC TATGGTATAA TGGAAGAACC AGATCTCACT 
CTAGTGAGTA ATAGTGACAT CAGCATCACT GAAACAGATC TTGCAAACTT AACCCTTGAA 
GACAGAGAAG ACAATGAAGC ACAGTTTTTT CAGGCAGGTG TCGTTCTGCC TACATCTTCA 
ATGGAAACCT CTGTTTGTGG AGCTGTATCA GAGCCTTATG TGGACCAACC CACAGTGGCA 
CCTTCAGCTA CATCAGGTAG TTTACAAGAA GCATTTATGA CAAGACAGAC ACTTACAGAA 
CGATCTTACC AGAGGCAGAG AGAGATCTGG AATAAAACTC GACTCCCTCA AACCAAAGTA 
TCCAAAGAGA AGCTACCTAC AGGTTGCACA GGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168912.1
CDS	78..6416
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_017313423.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313423.1

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGCA AGGAATCCCA CAGATGAGAA TGACTTGGAA 
ATGCAGAAGA GAGAACAGCT CGGAGGGTGG TTTCCTCACA CCCAAGGCCT TACCTGGGGA 
GACGCAGGTC AGGGGAGCGC TAATGGTGAG CAGCCACGTG CAGATGTCCA TGCTGAGCAC 
AATGGTGAGA GTCTGGCCAA AGAACTGAGT GGTAGAGCCT CAAAGCCACC TGTCTCCAAA 
GTTAAATGTG TCTTGGACTT AAACCAGCAT GAGCTTAGTA CTATACAGGA AGTAGAGTCA 
CCAGCAAGTG GCAGGATTTC CATGCCAGGT AAACAAGGAA AAGCAGAGTT TTATCAAGAC 
CGAGACCCAT TAAGGGTCTC AGTAAGCAGA GAACAAAGTT TCTTGGAGAG CCCACTGGCA 
CATGATCCAT TTGGTTGTCA CCAACCACCT GCCCAAGAGA ACAGCAAAAG CCATGACGAC 
AATGCTGAAG CAGTTAAAGT CAAGAAATCT GATGTTGAGG ATCATGCAGT ATTGAGTCAT 
GCTGTCTCAA AAGAAGAAGC GTGTACAAAC CTGGGTCCAC TTGGAAAGCC AGACGATGAG 
GCTGAAACAC AAGAGATTTC TCAGGAGCCA TTGTCTTCAG TAACTGTTTC TACTGGAAGC 
TTCTTAAGTT ATGAAATCAC AGATTTGAGC CTCACAGATC CAGAGTCATT TTCAGAACAG 
ACAGAGCATC TAGAACAAGA ATCTACCAAT AAGCAGGAAG AGACTGATCC TCTTAGTATT 
GCAGTCCCTT CAGTTATTTA TCAGCAACAG CATTCCTTAG GTGCTCATAA CTCTCTGTTG 
CCCATGGAGG AAGAAAGTAC ATCTGATCAT ACACATGTTC AGCAGATCAT GGACAATGAT 
GTAAATGAAG CAAATTTGAT ACCTGACAAA AGAGACTTTC AGGTTCCAGC TGTGGACCTT 
GACTTTCGAG AATTGGAACA CATATTTCCA CACTTGCATC GTCAGCTCTT TAAACCCTTA 
GAACCACATC TAGATTTTGA CTTATCATCC CCTGGGACAT CTCAAGAAGA CAGCGACTTT 
TACCAGCAGA GCTCAGAATC TTCATCTGAA AAACATGTTA AGGCATTATC CACAGGCACA 
ATCTGCTTCC CAGCACTGAC AGCCAAGTCA CATTCTCCTA ACCCAAGGCT GAACCAACTT 
GATATTAACC TGGCTCATGC AACAACTGAG GGATCTGAAC AGTCTTTTCA ACAGCTTCGA 
CCAGAATTCT CTTCACAGGA AAGCCAACAT GCTGACCTAC CCAGTATTTA TAGCATTGAA 
GCAAGAGGTC CTTCCCAACG CATGGAAAAC CAGAACTATT CTGAAATGTT ACAAAATAAG 
AAAAAAAGTC TCTCTCTCCA GCCCTCAACA GAGGATTTGA CTCCAGCTTG CAGTTCATCT 
GATACTGCTC TGTTTGATCA GTTACACCTG CAGCACAGCA CTCCGTGTGC TTCTGTCTCT 
AGTGAGTGCT CAGTAAAACT GTTAGAAAGC AGAGAAGAAG TGCTGGGATT TGAAGAGCTG 
TCAAGAAGGG CAGTTACAAT GTCACAAAGA CTCACTGAAG ATGAAAATGT AGTTCTACCT 
ATAAACCCAC ATGTAGGAAG AGTGGAGAAG GAGGCTTCAG TTCAGGGGTC TAATCCATTG 
AGTATTCAAA ATGAAAAGCC AATTCAGAAC TTCATTGAAA CTGACACTAC AGAAGCTGTA 
GGAAATGTAT GTCAGCTAGC ACAAGCAGAG CACATATTAA AATCCTGTCC ATTTCGGAGC 
CCTATTCCAA TCTGGGAAAC GGATACTGGC TATGGTATAA TGGAAGAACC AGATCTCACT 
CTAGTGAGTA ATAGTGACAT CAGCATCACT GAAACAGATC TTGCAAACTT AACCCTTGAA 
GACAGAGAAG ACAATGAAGC ACAGTTTTTT CAGGCAGGTG TCGTTCTGCC TACATCTTCA 
ATGGAAACCT CTGTTTGTGG AGCTGTATCA GAGCCTTATG TGGACCAACC CACAGTGGCA 
CCTTCAGCTA CATCAGGTAG TTTACAAGAA GCATTTATGA CAAGACAGAC ACTTACAGAA 
CGATCTTACC AGAGGCAGAG AGAGATCTGG AATAAAACTC GACTCCCTCA AACCAAAGTA 
TCCAAAGAGA AGCTACCTAC AGGTTGCACA GGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_017313423.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168912.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X14, mRNA.

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CloneID	OMu82854
Clone ID Related Accession (Same CDS sequence)	XM_017313423.1 , XM_017313423.2
Accession Version	XM_017313423.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017313423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGCA AGGAATCCCA CAGATGAGAA TGACTTGGAA 
ATGCAGAAGA GAGAACAGCT CGGAGGGTGG TTTCCTCACA CCCAAGGCCT TACCTGGGGA 
GACGCAGGTC AGGGGAGCGC TAATGGTGAG CAGCCACGTG CAGATGTCCA TGCTGAGCAC 
AATGGTGAGA GTCTGGCCAA AGAACTGAGT GGTAGAGCCT CAAAGCCACC TGTCTCCAAA 
GTTAAATGTG TCTTGGACTT AAACCAGCAT GAGCTTAGTA CTATACAGGA AGTAGAGTCA 
CCAGCAAGTG GCAGGATTTC CATGCCAGGT AAACAAGGAA AAGCAGAGTT TTATCAAGAC 
CGAGACCCAT TAAGGGTCTC AGTAAGCAGA GAACAAAGTT TCTTGGAGAG CCCACTGGCA 
CATGATCCAT TTGGTTGTCA CCAACCACCT GCCCAAGAGA ACAGCAAAAG CCATGACGAC 
AATGCTGAAG CAGTTAAAGT CAAGAAATCT GATGTTGAGG ATCATGCAGT ATTGAGTCAT 
GCTGTCTCAA AAGAAGAAGC GTGTACAAAC CTGGGTCCAC TTGGAAAGCC AGACGATGAG 
GCTGAAACAC AAGAGATTTC TCAGGAGCCA TTGTCTTCAG TAACTGTTTC TACTGGAAGC 
TTCTTAAGTT ATGAAATCAC AGATTTGAGC CTCACAGATC CAGAGTCATT TTCAGAACAG 
ACAGAGCATC TAGAACAAGA ATCTACCAAT AAGCAGGAAG AGACTGATCC TCTTAGTATT 
GCAGTCCCTT CAGTTATTTA TCAGCAACAG CATTCCTTAG GTGCTCATAA CTCTCTGTTG 
CCCATGGAGG AAGAAAGTAC ATCTGATCAT ACACATGTTC AGCAGATCAT GGACAATGAT 
GTAAATGAAG CAAATTTGAT ACCTGACAAA AGAGACTTTC AGGTTCCAGC TGTGGACCTT 
GACTTTCGAG AATTGGAACA CATATTTCCA CACTTGCATC GTCAGCTCTT TAAACCCTTA 
GAACCACATC TAGATTTTGA CTTATCATCC CCTGGGACAT CTCAAGAAGA CAGCGACTTT 
TACCAGCAGA GCTCAGAATC TTCATCTGAA AAACATGTTA AGGCATTATC CACAGGCACA 
ATCTGCTTCC CAGCACTGAC AGCCAAGTCA CATTCTCCTA ACCCAAGGCT GAACCAACTT 
GATATTAACC TGGCTCATGC AACAACTGAG GGATCTGAAC AGTCTTTTCA ACAGCTTCGA 
CCAGAATTCT CTTCACAGGA AAGCCAACAT GCTGACCTAC CCAGTATTTA TAGCATTGAA 
GCAAGAGGTC CTTCCCAACG CATGGAAAAC CAGAACTATT CTGAAATGTT ACAAAATAAG 
AAAAAAAGTC TCTCTCTCCA GCCCTCAACA GAGGATTTGA CTCCAGCTTG CAGTTCATCT 
GATACTGCTC TGTTTGATCA GTTACACCTG CAGCACAGCA CTCCGTGTGC TTCTGTCTCT 
AGTGAGTGCT CAGTAAAACT GTTAGAAAGC AGAGAAGAAG TGCTGGGATT TGAAGAGCTG 
TCAAGAAGGG CAGTTACAAT GTCACAAAGA CTCACTGAAG ATGAAAATGT AGTTCTACCT 
ATAAACCCAC ATGTAGGAAG AGTGGAGAAG GAGGCTTCAG TTCAGGGGTC TAATCCATTG 
AGTATTCAAA ATGAAAAGCC AATTCAGAAC TTCATTGAAA CTGACACTAC AGAAGCTGTA 
GGAAATGTAT GTCAGCTAGC ACAAGCAGAG CACATATTAA AATCCTGTCC ATTTCGGAGC 
CCTATTCCAA TCTGGGAAAC GGATACTGGC TATGGTATAA TGGAAGAACC AGATCTCACT 
CTAGTGAGTA ATAGTGACAT CAGCATCACT GAAACAGATC TTGCAAACTT AACCCTTGAA 
GACAGAGAAG ACAATGAAGC ACAGTTTTTT CAGGCAGGTG TCGTTCTGCC TACATCTTCA 
ATGGAAACCT CTGTTTGTGG AGCTGTATCA GAGCCTTATG TGGACCAACC CACAGTGGCA 
CCTTCAGCTA CATCAGGTAG TTTACAAGAA GCATTTATGA CAAGACAGAC ACTTACAGAA 
CGATCTTACC AGAGGCAGAG AGAGATCTGG AATAAAACTC GACTCCCTCA AACCAAAGTA 
TCCAAAGAGA AGCTACCTAC AGGTTGCACA GGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168912.1
CDS	107..6445
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_017313423.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313423.2

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGCA AGGAATCCCA CAGATGAGAA TGACTTGGAA 
ATGCAGAAGA GAGAACAGCT CGGAGGGTGG TTTCCTCACA CCCAAGGCCT TACCTGGGGA 
GACGCAGGTC AGGGGAGCGC TAATGGTGAG CAGCCACGTG CAGATGTCCA TGCTGAGCAC 
AATGGTGAGA GTCTGGCCAA AGAACTGAGT GGTAGAGCCT CAAAGCCACC TGTCTCCAAA 
GTTAAATGTG TCTTGGACTT AAACCAGCAT GAGCTTAGTA CTATACAGGA AGTAGAGTCA 
CCAGCAAGTG GCAGGATTTC CATGCCAGGT AAACAAGGAA AAGCAGAGTT TTATCAAGAC 
CGAGACCCAT TAAGGGTCTC AGTAAGCAGA GAACAAAGTT TCTTGGAGAG CCCACTGGCA 
CATGATCCAT TTGGTTGTCA CCAACCACCT GCCCAAGAGA ACAGCAAAAG CCATGACGAC 
AATGCTGAAG CAGTTAAAGT CAAGAAATCT GATGTTGAGG ATCATGCAGT ATTGAGTCAT 
GCTGTCTCAA AAGAAGAAGC GTGTACAAAC CTGGGTCCAC TTGGAAAGCC AGACGATGAG 
GCTGAAACAC AAGAGATTTC TCAGGAGCCA TTGTCTTCAG TAACTGTTTC TACTGGAAGC 
TTCTTAAGTT ATGAAATCAC AGATTTGAGC CTCACAGATC CAGAGTCATT TTCAGAACAG 
ACAGAGCATC TAGAACAAGA ATCTACCAAT AAGCAGGAAG AGACTGATCC TCTTAGTATT 
GCAGTCCCTT CAGTTATTTA TCAGCAACAG CATTCCTTAG GTGCTCATAA CTCTCTGTTG 
CCCATGGAGG AAGAAAGTAC ATCTGATCAT ACACATGTTC AGCAGATCAT GGACAATGAT 
GTAAATGAAG CAAATTTGAT ACCTGACAAA AGAGACTTTC AGGTTCCAGC TGTGGACCTT 
GACTTTCGAG AATTGGAACA CATATTTCCA CACTTGCATC GTCAGCTCTT TAAACCCTTA 
GAACCACATC TAGATTTTGA CTTATCATCC CCTGGGACAT CTCAAGAAGA CAGCGACTTT 
TACCAGCAGA GCTCAGAATC TTCATCTGAA AAACATGTTA AGGCATTATC CACAGGCACA 
ATCTGCTTCC CAGCACTGAC AGCCAAGTCA CATTCTCCTA ACCCAAGGCT GAACCAACTT 
GATATTAACC TGGCTCATGC AACAACTGAG GGATCTGAAC AGTCTTTTCA ACAGCTTCGA 
CCAGAATTCT CTTCACAGGA AAGCCAACAT GCTGACCTAC CCAGTATTTA TAGCATTGAA 
GCAAGAGGTC CTTCCCAACG CATGGAAAAC CAGAACTATT CTGAAATGTT ACAAAATAAG 
AAAAAAAGTC TCTCTCTCCA GCCCTCAACA GAGGATTTGA CTCCAGCTTG CAGTTCATCT 
GATACTGCTC TGTTTGATCA GTTACACCTG CAGCACAGCA CTCCGTGTGC TTCTGTCTCT 
AGTGAGTGCT CAGTAAAACT GTTAGAAAGC AGAGAAGAAG TGCTGGGATT TGAAGAGCTG 
TCAAGAAGGG CAGTTACAAT GTCACAAAGA CTCACTGAAG ATGAAAATGT AGTTCTACCT 
ATAAACCCAC ATGTAGGAAG AGTGGAGAAG GAGGCTTCAG TTCAGGGGTC TAATCCATTG 
AGTATTCAAA ATGAAAAGCC AATTCAGAAC TTCATTGAAA CTGACACTAC AGAAGCTGTA 
GGAAATGTAT GTCAGCTAGC ACAAGCAGAG CACATATTAA AATCCTGTCC ATTTCGGAGC 
CCTATTCCAA TCTGGGAAAC GGATACTGGC TATGGTATAA TGGAAGAACC AGATCTCACT 
CTAGTGAGTA ATAGTGACAT CAGCATCACT GAAACAGATC TTGCAAACTT AACCCTTGAA 
GACAGAGAAG ACAATGAAGC ACAGTTTTTT CAGGCAGGTG TCGTTCTGCC TACATCTTCA 
ATGGAAACCT CTGTTTGTGG AGCTGTATCA GAGCCTTATG TGGACCAACC CACAGTGGCA 
CCTTCAGCTA CATCAGGTAG TTTACAAGAA GCATTTATGA CAAGACAGAC ACTTACAGAA 
CGATCTTACC AGAGGCAGAG AGAGATCTGG AATAAAACTC GACTCCCTCA AACCAAAGTA 
TCCAAAGAGA AGCTACCTAC AGGTTGCACA GGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_030244412.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100272.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X9, mRNA.

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CloneID	OMu90201
Clone ID Related Accession (Same CDS sequence)	XM_030244412.1
Accession Version	XM_030244412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGTAAAC AAGGAAAAGC AGAGTTTTAT CAAGACCGAG ACCCATTAAG GGTCTCAGTA 
AGCAGAGAAC AAAGTTTCTT GGAGAGCCCA CTGGCACATG ATCCATTTGG TTGTCACCAA 
CCACCTGCCC AAGAGAACAG CAAAAGCCAT GACGACAATG CTGAAGCAGT TAAAGTCAAG 
AAATCTGATG TTGAGGATCA TGCAGTATTG AGTCATGCTG TCTCAAAAGA AGAAGCGTGT 
ACAAACCTGG GTCCACTTGG AAAGCCAGAC GATGAGGCTG AAACACAAGA GATTTCTCAG 
GAGCCATTGT CTTCAGTAAC TGTTTCTACT GGAAGCTTCT TAAGTTATGA AATCACAGAT 
TTGAGCCTCA CAGATCCAGT TCCAGCTGTG GACCTTGACT TTCGAGAATT GGAACACATA 
TTTCCACACT TGCATCGTCA GCTCTTTAAA CCCTTAGAAC CACATCTAGA TTTTGACTTA 
TCATCCCCTG GGACATCTCA AGAAGACAGC GACTTTTACC AGAGCTCAGA ATCTTCATCT 
GAAAAACATG TTAAGGCATT ATCCACAGGC ACAATCTGCT TCCCAGCACT GACAGCCAAG 
TCACATTCTC CTAACCCAAG GCTGAACCAA CTTGATATTA ACCTGGCTCA TGCAACAACT 
GAGGGATCTG AACAGTCTTT TCAACAGCTT CGACCAGAAT TCTCTTCACA GGAAAGCCAA 
CATGCTGACC TACCCAGTAT TTATAGCATT GAAGCAAGAG GTCCTTCCCA ACGCATGGAA 
AACCAGAACT ATTCTGAAAT GTTACAAAAT AAGAAAAAAA GTCTCTCTCT CCAGCCCTCA 
ACAGAGGATT TGACTCCAGC TTGCAGTTCA TCTGATACTG CTCTGTTTGA TCAGTTACAC 
CTGCAGCACA GCACTCCGTG TGCTTCTGTC TCTAGTGAGT GCTCAGTAAA ACTGTTAGAA 
AGCAGAGAAG AAGTGCTGGG ATTTGAAGAG CTGTCAAGAA GGGCAGTTAC AATGTCACAA 
AGACTCACTG AAGATGAAAA TGTAGTTCTA CCTATAAACC CACATGTAGG AAGAGTGGAG 
AAGGAGGCTT CAGTTCAGGG GTCTAATCCA TTGAGTATTC AAAATGAAAA GCCAATTCAG 
AACTTCATTG AAACTGACAC TACAGAAGCT GTAGGAAATG TATGTCAGCT AGCACAAGCA 
GAGCACATAT TAAAATCCTG TCCATTTCGG AGCCCTATTC CAATCTGGGA AACGGATACT 
GGCTATGGTA TAATGGAAGA ACCAGATCTC ACTCTAGTGA GTAATAGTGA CATCAGCATC 
ACTGAAACAG ATCTTGCAAA CTTAACCCTT GAAGACAGAG AAGACAATGA AGCACAGTTT 
TTTCAGGCAG GTGTCGTTCT GCCTACATCT TCAATGGAAA CCTCTGTTTG TGGAGCTGTA 
TCAGAGCCTT ATGTGGACCA ACCCACAGTG GCACCTTCAG CTACATCAGG TAGTTTACAA 
GAAGCATTTA TGACAAGACA GACACTTACA GAACGATCTT ACCAGAGGCA GAGAGAGATC 
TGGAATAAAA CTCGACTCCC TCAAACCAAA GTATCCAAAG AGAAGCTACC TACAGGTTGC 
ACAGGCAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100272.1
CDS	107..7258
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_030244412.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244412.1

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGTAAAC AAGGAAAAGC AGAGTTTTAT CAAGACCGAG ACCCATTAAG GGTCTCAGTA 
AGCAGAGAAC AAAGTTTCTT GGAGAGCCCA CTGGCACATG ATCCATTTGG TTGTCACCAA 
CCACCTGCCC AAGAGAACAG CAAAAGCCAT GACGACAATG CTGAAGCAGT TAAAGTCAAG 
AAATCTGATG TTGAGGATCA TGCAGTATTG AGTCATGCTG TCTCAAAAGA AGAAGCGTGT 
ACAAACCTGG GTCCACTTGG AAAGCCAGAC GATGAGGCTG AAACACAAGA GATTTCTCAG 
GAGCCATTGT CTTCAGTAAC TGTTTCTACT GGAAGCTTCT TAAGTTATGA AATCACAGAT 
TTGAGCCTCA CAGATCCAGT TCCAGCTGTG GACCTTGACT TTCGAGAATT GGAACACATA 
TTTCCACACT TGCATCGTCA GCTCTTTAAA CCCTTAGAAC CACATCTAGA TTTTGACTTA 
TCATCCCCTG GGACATCTCA AGAAGACAGC GACTTTTACC AGAGCTCAGA ATCTTCATCT 
GAAAAACATG TTAAGGCATT ATCCACAGGC ACAATCTGCT TCCCAGCACT GACAGCCAAG 
TCACATTCTC CTAACCCAAG GCTGAACCAA CTTGATATTA ACCTGGCTCA TGCAACAACT 
GAGGGATCTG AACAGTCTTT TCAACAGCTT CGACCAGAAT TCTCTTCACA GGAAAGCCAA 
CATGCTGACC TACCCAGTAT TTATAGCATT GAAGCAAGAG GTCCTTCCCA ACGCATGGAA 
AACCAGAACT ATTCTGAAAT GTTACAAAAT AAGAAAAAAA GTCTCTCTCT CCAGCCCTCA 
ACAGAGGATT TGACTCCAGC TTGCAGTTCA TCTGATACTG CTCTGTTTGA TCAGTTACAC 
CTGCAGCACA GCACTCCGTG TGCTTCTGTC TCTAGTGAGT GCTCAGTAAA ACTGTTAGAA 
AGCAGAGAAG AAGTGCTGGG ATTTGAAGAG CTGTCAAGAA GGGCAGTTAC AATGTCACAA 
AGACTCACTG AAGATGAAAA TGTAGTTCTA CCTATAAACC CACATGTAGG AAGAGTGGAG 
AAGGAGGCTT CAGTTCAGGG GTCTAATCCA TTGAGTATTC AAAATGAAAA GCCAATTCAG 
AACTTCATTG AAACTGACAC TACAGAAGCT GTAGGAAATG TATGTCAGCT AGCACAAGCA 
GAGCACATAT TAAAATCCTG TCCATTTCGG AGCCCTATTC CAATCTGGGA AACGGATACT 
GGCTATGGTA TAATGGAAGA ACCAGATCTC ACTCTAGTGA GTAATAGTGA CATCAGCATC 
ACTGAAACAG ATCTTGCAAA CTTAACCCTT GAAGACAGAG AAGACAATGA AGCACAGTTT 
TTTCAGGCAG GTGTCGTTCT GCCTACATCT TCAATGGAAA CCTCTGTTTG TGGAGCTGTA 
TCAGAGCCTT ATGTGGACCA ACCCACAGTG GCACCTTCAG CTACATCAGG TAGTTTACAA 
GAAGCATTTA TGACAAGACA GACACTTACA GAACGATCTT ACCAGAGGCA GAGAGAGATC 
TGGAATAAAA CTCGACTCCC TCAAACCAAA GTATCCAAAG AGAAGCTACC TACAGGTTGC 
ACAGGCAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_030244413.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100273.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X10, mRNA.

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CloneID	OMu90202
Clone ID Related Accession (Same CDS sequence)	XM_030244413.1
Accession Version	XM_030244413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGCAGAGCT CAGAATCTTC ATCTGAAAAA 
CATGTTAAGG CATTATCCAC AGGCACAATC TGCTTCCCAG CACTGACAGC CAAGTCACAT 
TCTCCTAACC CAAGGCTGAA CCAACTTGAT ATTAACCTGG CTCATGCAAC AACTGAGGGA 
TCTGAACAGT CTTTTCAACA GCTTCGACCA GAATTCTCTT CACAGGAAAG CCAACATGCT 
GACCTACCCA GTATTTATAG CATTGAAGCA AGAGGTCCTT CCCAACGCAT GGAAAACCAG 
AACTATTCTG AAATGTTACA AAATAAGAAA AAAAGTCTCT CTCTCCAGCC CTCAACAGAG 
GATTTGACTC CAGCTTGCAG TTCATCTGAT ACTGCTCTGT TTGATCAGTT ACACCTGCAG 
CACAGCACTC CGTGTGCTTC TGTCTCTAGT GAGTGCTCAG TAAAACTGTT AGAAAGCAGA 
GAAGAAGTGC TGGGATTTGA AGAGCTGTCA AGAAGGGCAG TTACAATGTC ACAAAGACTC 
ACTGAAGATG AAAATGTAGT TCTACCTATA AACCCACATG TAGGAAGAGT GGAGAAGGAG 
GCTTCAGTTC AGGGGTCTAA TCCATTGAGT ATTCAAAATG AAAAGCCAAT TCAGAACTTC 
ATTGAAACTG ACACTACAGA AGCTGTAGGA AATGTATGTC AGCTAGCACA AGCAGAGCAC 
ATATTAAAAT CCTGTCCATT TCGGAGCCCT ATTCCAATCT GGGAAACGGA TACTGGCTAT 
GGTATAATGG AAGAACCAGA TCTCACTCTA GTGAGTAATA GTGACATCAG CATCACTGAA 
ACAGATCTTG CAAACTTAAC CCTTGAAGAC AGAGAAGACA ATGAAGCACA GTTTTTTCAG 
GCAGGTGTCG TTCTGCCTAC ATCTTCAATG GAAACCTCTG TTTGTGGAGC TGTATCAGAG 
CCTTATGTGG ACCAACCCAC AGTGGCACCT TCAGCTACAT CAGGTAGTTT ACAAGAAGCA 
TTTATGACAA GACAGACACT TACAGAACGA TCTTACCAGA GGCAGAGAGA GATCTGGAAT 
AAAACTCGAC TCCCTCAAAC CAAAGTATCC AAAGAGAAGC TACCTACAGG TTGCACAGGC 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100273.1
CDS	107..7252
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_030244413.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244413.1

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGCAGAGCT CAGAATCTTC ATCTGAAAAA 
CATGTTAAGG CATTATCCAC AGGCACAATC TGCTTCCCAG CACTGACAGC CAAGTCACAT 
TCTCCTAACC CAAGGCTGAA CCAACTTGAT ATTAACCTGG CTCATGCAAC AACTGAGGGA 
TCTGAACAGT CTTTTCAACA GCTTCGACCA GAATTCTCTT CACAGGAAAG CCAACATGCT 
GACCTACCCA GTATTTATAG CATTGAAGCA AGAGGTCCTT CCCAACGCAT GGAAAACCAG 
AACTATTCTG AAATGTTACA AAATAAGAAA AAAAGTCTCT CTCTCCAGCC CTCAACAGAG 
GATTTGACTC CAGCTTGCAG TTCATCTGAT ACTGCTCTGT TTGATCAGTT ACACCTGCAG 
CACAGCACTC CGTGTGCTTC TGTCTCTAGT GAGTGCTCAG TAAAACTGTT AGAAAGCAGA 
GAAGAAGTGC TGGGATTTGA AGAGCTGTCA AGAAGGGCAG TTACAATGTC ACAAAGACTC 
ACTGAAGATG AAAATGTAGT TCTACCTATA AACCCACATG TAGGAAGAGT GGAGAAGGAG 
GCTTCAGTTC AGGGGTCTAA TCCATTGAGT ATTCAAAATG AAAAGCCAAT TCAGAACTTC 
ATTGAAACTG ACACTACAGA AGCTGTAGGA AATGTATGTC AGCTAGCACA AGCAGAGCAC 
ATATTAAAAT CCTGTCCATT TCGGAGCCCT ATTCCAATCT GGGAAACGGA TACTGGCTAT 
GGTATAATGG AAGAACCAGA TCTCACTCTA GTGAGTAATA GTGACATCAG CATCACTGAA 
ACAGATCTTG CAAACTTAAC CCTTGAAGAC AGAGAAGACA ATGAAGCACA GTTTTTTCAG 
GCAGGTGTCG TTCTGCCTAC ATCTTCAATG GAAACCTCTG TTTGTGGAGC TGTATCAGAG 
CCTTATGTGG ACCAACCCAC AGTGGCACCT TCAGCTACAT CAGGTAGTTT ACAAGAAGCA 
TTTATGACAA GACAGACACT TACAGAACGA TCTTACCAGA GGCAGAGAGA GATCTGGAAT 
AAAACTCGAC TCCCTCAAAC CAAAGTATCC AAAGAGAAGC TACCTACAGG TTGCACAGGC 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_030244415.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100275.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X12, mRNA.

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CloneID	OMu90203
Clone ID Related Accession (Same CDS sequence)	XM_030244415.1 , XM_030244414.1
Accession Version	XM_030244415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGA TCCACTTGGC CCAAAACCAG GAGCGATTGA TGGAAGAACT CAAACAGCTG 
CAGAAAGAGG ACCTGGCCCG TAGGAGGCAG ACCGTGGCAC AGATGCCGCC ACAGATGCTG 
GAGTTGCCAT ACCGACGGAG TGAGATGAAG GAAGACAGGC AGCGAGAGCT GGAGTTTGCC 
TTTGAAGATA TGTACAATGC AGATAGGAAG GTGAAAGGAA ACCTGATTTT ACATCTTAAG 
CCAGAACCTT TGCCTACAAT CTCTGATCAG CTCCAAGATG AAGAACTGGA CCTTTCAATG 
GAACAAGAAA ATCAAGTGCC CTTGGCAGCA AAGATCCAAC AAATCCCCTC AAGAATTCTT 
TTTAAAAGAT TATTAAACAA GATTAGAAGT CAAAAATCTC TCTGGACAAT TAAATCTGTG 
TCTGAGGATG AAGGTGAGGT GACTTCGAGC ATCATTGAGA TTGAGAGTAA AGTGCCAAGC 
GTGGACTCGG GAGCCATTAT CACAGAGGAG AGAACAGCGG CGTCCTTTGA GCAGGAGCAA 
GTCACAGACA GTGATAGGCT GACGATAGAA TCTGGACCAC TGAGCAGTGA AGATAAGCCA 
TTGTACTACA AAGCGGGGAC TGGAAGGGAA CAAGCAATGG CAGTGTCTCC ACCTGCCACA 
GCTGTGGCTC AGAGTTCAGT CCTGCTTCAT CCTCAGGAAG AGGCAGTCAG AATTCGAATG 
TCATTAAGGC GCAAACAGAT AATGGAGATA GAAGAGCAGA AGCAGAAGCA GTTGGAATTA 
CTTGAACAAA TTGAGCAACA GAAGTTAAGG TTAGAAACTG ATTGTTTCCG GGCTCAACTG 
GAAGAGCAAA GAAAGCAAGC TGATCAGCCT GAGGTTTGCT GTGCTCCAAT GTCACATGCT 
ATGATTTCTG ATGAAGACTC TCACAGGCAG ATGATTCGTA ACTATCAACA TCAGCTTTTA 
CAACAAAACA GGTTACACAA GGAGACTGTT GAAACTGCCA GGAAACGATT ACTCGAATAC 
CAAACTGTAT TAAAAGAAAG ATCCCCATCC CTGTCAGCCT CAGCACTGGT ACCTGATTCT 
GTTGTGTCAG GGCCACCACA GCAATCCTAC AAGCCTGCTG CTGCTTCAGA CTCTTGGGAT 
CCAAGCCAGA GACTGAAACT AAGTCCTAGC AAATATCAAC CTGTGCAACC CTCACAGATC 
CCTGCATTAG AGCAGAGTCA TATTCAGGTA CCAAGACATG GTCACATTAC ACAGAGGCAA 
GGAAAAATGG CTGTATCAGA AATGTTAGGT AAGCAGCCCG TGGAGTCACA AGAACGTCAG 
TGGCAGTTCT CTCAGGTGGA AACACATCAG GGAGACTATG AGTTCGTCCT TAAAGATTCT 
CATTCACTTT CAAGGACTTT GTCTTATGTT AGGCCACAAA CATTACAAGA TGCTAGAGAA 
GTATCTAAAC CTCCTAGGGT AATAATTTGT CAAAGTCTAG ATTCCCAACA GATATCATCA 
GAGGATAGTG AAAATATATC TTCTAAGCCA AGTGAACCGT CTCCTTTTCT CCCACTGGTG 
CCTGAACGCC CTTTTACTAG TCTCCCAGTT AAATTCCATT CTGGAACAAT TCACAAACCC 
TTTACGACCA TAAACCAGTC TGTAATCAGT CAAATGCATG ATCAACCTTT GTCATCCTCA 
GAGACTATTA CAGCTCAGCA GGGTGACTTA AGATTTCTTC AAGAACAGTT AGAACTACAG 
AAGAAAGTTC TTCAGGCAAG ACAAGAGGCT CGGGAAAAGC TGCTTTTGTG CACACAGAAA 
GAACTGGGAC AGCAGACTGG CCTCCCAGTG TTCCTCCCTT CACCAGCTGG AAATATATTT 
TCTTCATTAC CCTCTGCCTC AGCTGAATCA GGAAACTTTC AGACATCTTC AACAAAAAGT 
GATGCCACTG TTTCCTCAGA CAATATGGAC AGGCTTTGGG ATTCTTCACA ACCCATCTCA 
TCACAGCAAA CTCATTTAGA ATTTCTCCAA GAGCAGTCCA GTGTTGAGAC GGATAACCTT 
CAGGCTAGGC GGGAAGCCCA GGAGGTCTTG TTTGCACATA CACAAAATAC GCTGGAAAAA 
ATTGTACGTT CTGAACAAGC TGGGTCTTCT TTGCCACATC AGGTAGCTCA GCAGTCATTT 
AGTTCACTAA CATTGGCTGA TACACAATCT AAAAAAATCC AGAAACAGCC TTTACCTGCA 
AACAAGAAAG GGCTACTCCC AAGCCAGTCT GAAGTCTCAA AAGCTCAGGA TGGATCTTCA 
GGTTTTCTAC AGCAGACCCT ACCTCTCCAG AACACTTTAA AGTTGCTTCA AGAGCAGCTG 
ACTAGACAGA GGAGTATGAT TCCGCCTAGA CGTGATGGTC AAGAGACATT ACTTTTATAT 
AAAGAAAGCT GTTCAGAAGA CAGTGAGGCT GGGCCAGTAG AATCACTCTC CTCCGTGGTT 
GTTCAGCATG CAGATGCTTC TCGTGCAGTT TCAGAAGTAC CTAAGCGGCT CCAGGACGTG 
TATTCATCTG AGGAAGAAAA TAGAGTTCTC TCCAGTCATT TGATCACCCA TGGCTTTCCA 
CAGCACAGCC TGCAAAGACA AGAACATTTT ACACCACTCC AGGAAGAGAC ACACATTCAG 
AGGCTTATAC TTGGTGCTAG AAAAAATAAT GAGGAATTTG CTCCCAAGCA AAATGAATTA 
GAAAAGGGGC TCTGCTCTCA ACAGACTGAT GCCTTGTCTT CTCCATCCCA AGTCACCGAC 
TGGGGAACAT CCCGGGGGTC TGTATCAGTC AGAAGTGATA GGACAGACCC CTTAAGGCAT 
TTTAAGATTC CAGCGTTTCG GGAAAGACTA GTTAGAGTTT CACAACATAC ATTCCCTCTC 
CAAGATAATT TGCAGGAACA CCAAGAATGG GTAGACACAG AAAAAGAGAG CTTTCAATCT 
AGTCCTCTAA CTCCAGAAAA TCCATCTTCT CAACAGACAG GTTTTTCGTC CTTCAAAGCC 
TCATTAAGAC TGCCATCGTG TGTGTCCTTG CCATCTGCTG ACTCTGGTAT CACACAGCAC 
CCTCTCTCAA CAGAGAGTGA CAGTAAAGTC AAGTCAAGCC ATCTTCAGAT ACCAGAACTG 
CAGCATAGAC TTTCGAAGAT ATCCCAGCTC ATCCCGCCTC AACAAGACAG TTTGAAGGCC 
CTTCAAGAAC AATTAGCTAC ACAAAGGGAA GCCATCATCC ACTCTAGACA AGAAGCTCAT 
GAAGAAACAC TAAGAGAATG GAAGGAAAAA ATATTCCCAG AGCAGGTTGG TCCATTTTCT 
CCCCTGATAC CACAACATTC ACTTGCTTCA TTCCCTGTTT CTGATACTGA AAGAGCACAA 
GAACTGTGTT CAACCAACAG TGACACCATA TCTTCAGGTT ATCCTGAGAT GCTGGAGTTG 
CCTGACAGGA CATTGGGTTT ATCACATACT GCTTTACCTC AGCAGAATAA TCTGACTGCA 
CATCCAGAAC ACCTTCATGC ACAAACAAAT TTTTTTCACT CTACTGAGAA AGCCCAAGAA 
GGGTTGGTGT TTCCCAGACC ATGTCAATTT GAAGAGATGT CTGCTGAGCA TTTTATCCAG 
CCTCAACATG ATGACTTAAA GGCACTTCAA CAGCAGTTAG ATATGCAAAG GGAAGCCATT 
AGATCTGGCC AGGAAATGCA AGAGAAAATG CTTTTGCAAA GGTTAAATAA ATTGGAGCAA 
AGGATATCTT CCAAACAAAT TAGCTCCTCT CTATTTTCAT CCCAGGTAGC ACTGCCCATT 
GCCAATTCTG ACGGGACCTT ACAGTCTTTC CCAACTAAAA GTAACGAGAC TGAGCTGCTT 
GGGTCCCAAG ATGAGTATCT GAGCTTTTCA CAGCCTCGTC TGCCTCTGCA GAATAACATG 
ACAGAGCAGT TGGACTTAGA AAAGGTATTT CATAAAGAAC TGCTTTTACA CAAACAAAAG 
AGCCAAAACA AAAGTGAATC TTCTGAGCAT TCCCTCCCTC CTTTGTTTCT GTCCAAGGAA 
ATAGAGCATC CATTCATTTC ACTACCTTTT GCAGAAAGTA AATCTAAAAG CATTTGTGAG 
TTGTATTTAT CTGACAAGAA GCATGCAGCT CCTAATGATG CTGTGATCCC AAGATTGCAG 
GACAGGCTTT TAAGCTGTTC CCAACCTGTC TTAACTCAGC AAGATAACAT GAGTCTTCAG 
AAGCAGCTGA ATCTACAAAG AGAAACTCTA CACTCTAGGC AAAAAGCCCA AGAAGAATTG 
CTTGTGCAGA GACAGACATC CTTACAGCAG CAAATCCAAA GACACAGAGA GACTTTGAAA 
AATTTCTTTA ATGTCAGTCA GGCAAGGAAT CCCACAGATG AGAATGACTT GGAAATGCAG 
AAGAGAGAAC AGCTCGGAGG GTGGTTTCCT CACACCCAAG GCCTTACCTG GGGAGACGCA 
GGTCAGGGGA GCGCTAATGG TGAGCAGCCA CGTGCAGATG TCCATGCTGA GCACAATGGT 
GAGAGTCTGG CCAAAGAACT GAGTGGTAGA GCCTCAAAGC CACCTGTCTC CAAAGTTAAA 
TGTGTCTTGG ACTTAAACCA GCATGAGCTT AGTACTATAC AGGAAGTAGA GTCACCAGCA 
AGTGGCAGGA TTTCCATGCC AGGTAAACAA GGAAAAGCAG AGTTTTATCA AGACCGAGAC 
CCATTAAGGG TCTCAGTAAG CAGAGAACAA AGTTTCTTGG AGAGCCCACT GGCACATGAT 
CCATTTGGTT GTCACCAACC ACCTGCCCAA GAGAACAGCA AAAGCCATGA CGACAATGCT 
GAAGCAGTTA AAGTCAAGAA ATCTGATGTT GAGGATCATG CAGTATTGAG TCATGCTGTC 
TCAAAAGAAG AAGCGTGTAC AAACCTGGGT CCACTTGGAA AGCCAGACGA TGAGGCTGAA 
ACACAAGAGA TTTCTCAGGA GCCATTGTCT TCAGTAACTG TTTCTACTGG AAGCTTCTTA 
AGTTATGAAA TCACAGATTT GAGCCTCACA GATCCAGAGT CATTTTCAGA ACAGACAGAG 
CATCTAGAAC AAGAATCTAC CAATAAGCAG GAAGAGACTG ATCCTCTTAG TATTGCAGTC 
CCTTCAGTTA TTTATCAGCA ACAGCATTCC TTAGGTGCTC ATAACTCTCT GTTGCCCATG 
GAGGAAGAAA GTACATCTGA TCATACACAT GTTCAGCAGA TCATGGACAA TGATGTAAAT 
GAAGCAAATT TGATACCTGA CAAAAGAGAC TTTCAGGTTC CAGCTGTGGA CCTTGACTTT 
CGAGAATTGG AACACATATT TCCACACTTG CATCGTCAGC TCTTTAAACC CTTAGAACCA 
CATCTAGATT TTGACTTATC ATCCCCTGGG ACATCTCAAG AAGACAGCGA CTTTTACCAG 
CAGAGCTCAG AATCTTCATC TGAAAAACAT GTTAAGGCAT TATCCACAGG CACAATCTGC 
TTCCCAGCAC TGACAGCCAA GTCACATTCT CCTAACCCAA GGCTGAACCA ACTTGATATT 
AACCTGGCTC ATGCAACAAC TGAGGGATCT GAACAGTCTT TTCAACAGCT TCGACCAGAA 
TTCTCTTCAC AGGAAAGCCA ACATGCTGAC CTACCCAGTA TTTATAGCAT TGAAGCAAGA 
GGTCCTTCCC AACGCATGGA AAACCAGAAC TATTCTGAAA TGTTACAAAA TAAGAAAAAA 
AGTCTCTCTC TCCAGCCCTC AACAGAGGAT TTGACTCCAG CTTGCAGTTC ATCTGATACT 
GCTCTGTTTG ATCAGTTACA CCTGCAGCAC AGCACTCCGT GTGCTTCTGT CTCTAGTGAG 
TGCTCAGTAA AACTGTTAGA AAGCAGAGAA GAAGTGCTGG GATTTGAAGA GCTGTCAAGA 
AGGGCAGTTA CAATGTCACA AAGACTCACT GAAGATGAAA ATGTAGTTCT ACCTATAAAC 
CCACATGTAG GAAGAGTGGA GAAGGAGGCT TCAGTTCAGG GGTCTAATCC ATTGAGTATT 
CAAAATGAAA AGCCAATTCA GAACTTCATT GAAACTGACA CTACAGAAGC TGTAGGAAAT 
GTATGTCAGC TAGCACAAGC AGAGCACATA TTAAAATCCT GTCCATTTCG GAGCCCTATT 
CCAATCTGGG AAACGGATAC TGGCTATGGT ATAATGGAAG AACCAGATCT CACTCTAGTG 
AGTAATAGTG ACATCAGCAT CACTGAAACA GATCTTGCAA ACTTAACCCT TGAAGACAGA 
GAAGACAATG AAGCACAGTT TTTTCAGGCA GGTGTCGTTC TGCCTACATC TTCAATGGAA 
ACCTCTGTTT GTGGAGCTGT ATCAGAGCCT TATGTGGACC AACCCACAGT GGCACCTTCA 
GCTACATCAG GTAGTTTACA AGAAGCATTT ATGACAAGAC AGACACTTAC AGAACGATCT 
TACCAGAGGC AGAGAGAGAT CTGGAATAAA ACTCGACTCC CTCAAACCAA AGTATCCAAA 
GAGAAGCTAC CTACAGGTTG CACAGGCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100275.1
CDS	841..7353
Translation	MKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_030244415.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244415.1

ORF Insert Sequence:

ATGAAAAAGA TCCACTTGGC CCAAAACCAG GAGCGATTGA TGGAAGAACT CAAACAGCTG 
CAGAAAGAGG ACCTGGCCCG TAGGAGGCAG ACCGTGGCAC AGATGCCGCC ACAGATGCTG 
GAGTTGCCAT ACCGACGGAG TGAGATGAAG GAAGACAGGC AGCGAGAGCT GGAGTTTGCC 
TTTGAAGATA TGTACAATGC AGATAGGAAG GTGAAAGGAA ACCTGATTTT ACATCTTAAG 
CCAGAACCTT TGCCTACAAT CTCTGATCAG CTCCAAGATG AAGAACTGGA CCTTTCAATG 
GAACAAGAAA ATCAAGTGCC CTTGGCAGCA AAGATCCAAC AAATCCCCTC AAGAATTCTT 
TTTAAAAGAT TATTAAACAA GATTAGAAGT CAAAAATCTC TCTGGACAAT TAAATCTGTG 
TCTGAGGATG AAGGTGAGGT GACTTCGAGC ATCATTGAGA TTGAGAGTAA AGTGCCAAGC 
GTGGACTCGG GAGCCATTAT CACAGAGGAG AGAACAGCGG CGTCCTTTGA GCAGGAGCAA 
GTCACAGACA GTGATAGGCT GACGATAGAA TCTGGACCAC TGAGCAGTGA AGATAAGCCA 
TTGTACTACA AAGCGGGGAC TGGAAGGGAA CAAGCAATGG CAGTGTCTCC ACCTGCCACA 
GCTGTGGCTC AGAGTTCAGT CCTGCTTCAT CCTCAGGAAG AGGCAGTCAG AATTCGAATG 
TCATTAAGGC GCAAACAGAT AATGGAGATA GAAGAGCAGA AGCAGAAGCA GTTGGAATTA 
CTTGAACAAA TTGAGCAACA GAAGTTAAGG TTAGAAACTG ATTGTTTCCG GGCTCAACTG 
GAAGAGCAAA GAAAGCAAGC TGATCAGCCT GAGGTTTGCT GTGCTCCAAT GTCACATGCT 
ATGATTTCTG ATGAAGACTC TCACAGGCAG ATGATTCGTA ACTATCAACA TCAGCTTTTA 
CAACAAAACA GGTTACACAA GGAGACTGTT GAAACTGCCA GGAAACGATT ACTCGAATAC 
CAAACTGTAT TAAAAGAAAG ATCCCCATCC CTGTCAGCCT CAGCACTGGT ACCTGATTCT 
GTTGTGTCAG GGCCACCACA GCAATCCTAC AAGCCTGCTG CTGCTTCAGA CTCTTGGGAT 
CCAAGCCAGA GACTGAAACT AAGTCCTAGC AAATATCAAC CTGTGCAACC CTCACAGATC 
CCTGCATTAG AGCAGAGTCA TATTCAGGTA CCAAGACATG GTCACATTAC ACAGAGGCAA 
GGAAAAATGG CTGTATCAGA AATGTTAGGT AAGCAGCCCG TGGAGTCACA AGAACGTCAG 
TGGCAGTTCT CTCAGGTGGA AACACATCAG GGAGACTATG AGTTCGTCCT TAAAGATTCT 
CATTCACTTT CAAGGACTTT GTCTTATGTT AGGCCACAAA CATTACAAGA TGCTAGAGAA 
GTATCTAAAC CTCCTAGGGT AATAATTTGT CAAAGTCTAG ATTCCCAACA GATATCATCA 
GAGGATAGTG AAAATATATC TTCTAAGCCA AGTGAACCGT CTCCTTTTCT CCCACTGGTG 
CCTGAACGCC CTTTTACTAG TCTCCCAGTT AAATTCCATT CTGGAACAAT TCACAAACCC 
TTTACGACCA TAAACCAGTC TGTAATCAGT CAAATGCATG ATCAACCTTT GTCATCCTCA 
GAGACTATTA CAGCTCAGCA GGGTGACTTA AGATTTCTTC AAGAACAGTT AGAACTACAG 
AAGAAAGTTC TTCAGGCAAG ACAAGAGGCT CGGGAAAAGC TGCTTTTGTG CACACAGAAA 
GAACTGGGAC AGCAGACTGG CCTCCCAGTG TTCCTCCCTT CACCAGCTGG AAATATATTT 
TCTTCATTAC CCTCTGCCTC AGCTGAATCA GGAAACTTTC AGACATCTTC AACAAAAAGT 
GATGCCACTG TTTCCTCAGA CAATATGGAC AGGCTTTGGG ATTCTTCACA ACCCATCTCA 
TCACAGCAAA CTCATTTAGA ATTTCTCCAA GAGCAGTCCA GTGTTGAGAC GGATAACCTT 
CAGGCTAGGC GGGAAGCCCA GGAGGTCTTG TTTGCACATA CACAAAATAC GCTGGAAAAA 
ATTGTACGTT CTGAACAAGC TGGGTCTTCT TTGCCACATC AGGTAGCTCA GCAGTCATTT 
AGTTCACTAA CATTGGCTGA TACACAATCT AAAAAAATCC AGAAACAGCC TTTACCTGCA 
AACAAGAAAG GGCTACTCCC AAGCCAGTCT GAAGTCTCAA AAGCTCAGGA TGGATCTTCA 
GGTTTTCTAC AGCAGACCCT ACCTCTCCAG AACACTTTAA AGTTGCTTCA AGAGCAGCTG 
ACTAGACAGA GGAGTATGAT TCCGCCTAGA CGTGATGGTC AAGAGACATT ACTTTTATAT 
AAAGAAAGCT GTTCAGAAGA CAGTGAGGCT GGGCCAGTAG AATCACTCTC CTCCGTGGTT 
GTTCAGCATG CAGATGCTTC TCGTGCAGTT TCAGAAGTAC CTAAGCGGCT CCAGGACGTG 
TATTCATCTG AGGAAGAAAA TAGAGTTCTC TCCAGTCATT TGATCACCCA TGGCTTTCCA 
CAGCACAGCC TGCAAAGACA AGAACATTTT ACACCACTCC AGGAAGAGAC ACACATTCAG 
AGGCTTATAC TTGGTGCTAG AAAAAATAAT GAGGAATTTG CTCCCAAGCA AAATGAATTA 
GAAAAGGGGC TCTGCTCTCA ACAGACTGAT GCCTTGTCTT CTCCATCCCA AGTCACCGAC 
TGGGGAACAT CCCGGGGGTC TGTATCAGTC AGAAGTGATA GGACAGACCC CTTAAGGCAT 
TTTAAGATTC CAGCGTTTCG GGAAAGACTA GTTAGAGTTT CACAACATAC ATTCCCTCTC 
CAAGATAATT TGCAGGAACA CCAAGAATGG GTAGACACAG AAAAAGAGAG CTTTCAATCT 
AGTCCTCTAA CTCCAGAAAA TCCATCTTCT CAACAGACAG GTTTTTCGTC CTTCAAAGCC 
TCATTAAGAC TGCCATCGTG TGTGTCCTTG CCATCTGCTG ACTCTGGTAT CACACAGCAC 
CCTCTCTCAA CAGAGAGTGA CAGTAAAGTC AAGTCAAGCC ATCTTCAGAT ACCAGAACTG 
CAGCATAGAC TTTCGAAGAT ATCCCAGCTC ATCCCGCCTC AACAAGACAG TTTGAAGGCC 
CTTCAAGAAC AATTAGCTAC ACAAAGGGAA GCCATCATCC ACTCTAGACA AGAAGCTCAT 
GAAGAAACAC TAAGAGAATG GAAGGAAAAA ATATTCCCAG AGCAGGTTGG TCCATTTTCT 
CCCCTGATAC CACAACATTC ACTTGCTTCA TTCCCTGTTT CTGATACTGA AAGAGCACAA 
GAACTGTGTT CAACCAACAG TGACACCATA TCTTCAGGTT ATCCTGAGAT GCTGGAGTTG 
CCTGACAGGA CATTGGGTTT ATCACATACT GCTTTACCTC AGCAGAATAA TCTGACTGCA 
CATCCAGAAC ACCTTCATGC ACAAACAAAT TTTTTTCACT CTACTGAGAA AGCCCAAGAA 
GGGTTGGTGT TTCCCAGACC ATGTCAATTT GAAGAGATGT CTGCTGAGCA TTTTATCCAG 
CCTCAACATG ATGACTTAAA GGCACTTCAA CAGCAGTTAG ATATGCAAAG GGAAGCCATT 
AGATCTGGCC AGGAAATGCA AGAGAAAATG CTTTTGCAAA GGTTAAATAA ATTGGAGCAA 
AGGATATCTT CCAAACAAAT TAGCTCCTCT CTATTTTCAT CCCAGGTAGC ACTGCCCATT 
GCCAATTCTG ACGGGACCTT ACAGTCTTTC CCAACTAAAA GTAACGAGAC TGAGCTGCTT 
GGGTCCCAAG ATGAGTATCT GAGCTTTTCA CAGCCTCGTC TGCCTCTGCA GAATAACATG 
ACAGAGCAGT TGGACTTAGA AAAGGTATTT CATAAAGAAC TGCTTTTACA CAAACAAAAG 
AGCCAAAACA AAAGTGAATC TTCTGAGCAT TCCCTCCCTC CTTTGTTTCT GTCCAAGGAA 
ATAGAGCATC CATTCATTTC ACTACCTTTT GCAGAAAGTA AATCTAAAAG CATTTGTGAG 
TTGTATTTAT CTGACAAGAA GCATGCAGCT CCTAATGATG CTGTGATCCC AAGATTGCAG 
GACAGGCTTT TAAGCTGTTC CCAACCTGTC TTAACTCAGC AAGATAACAT GAGTCTTCAG 
AAGCAGCTGA ATCTACAAAG AGAAACTCTA CACTCTAGGC AAAAAGCCCA AGAAGAATTG 
CTTGTGCAGA GACAGACATC CTTACAGCAG CAAATCCAAA GACACAGAGA GACTTTGAAA 
AATTTCTTTA ATGTCAGTCA GGCAAGGAAT CCCACAGATG AGAATGACTT GGAAATGCAG 
AAGAGAGAAC AGCTCGGAGG GTGGTTTCCT CACACCCAAG GCCTTACCTG GGGAGACGCA 
GGTCAGGGGA GCGCTAATGG TGAGCAGCCA CGTGCAGATG TCCATGCTGA GCACAATGGT 
GAGAGTCTGG CCAAAGAACT GAGTGGTAGA GCCTCAAAGC CACCTGTCTC CAAAGTTAAA 
TGTGTCTTGG ACTTAAACCA GCATGAGCTT AGTACTATAC AGGAAGTAGA GTCACCAGCA 
AGTGGCAGGA TTTCCATGCC AGGTAAACAA GGAAAAGCAG AGTTTTATCA AGACCGAGAC 
CCATTAAGGG TCTCAGTAAG CAGAGAACAA AGTTTCTTGG AGAGCCCACT GGCACATGAT 
CCATTTGGTT GTCACCAACC ACCTGCCCAA GAGAACAGCA AAAGCCATGA CGACAATGCT 
GAAGCAGTTA AAGTCAAGAA ATCTGATGTT GAGGATCATG CAGTATTGAG TCATGCTGTC 
TCAAAAGAAG AAGCGTGTAC AAACCTGGGT CCACTTGGAA AGCCAGACGA TGAGGCTGAA 
ACACAAGAGA TTTCTCAGGA GCCATTGTCT TCAGTAACTG TTTCTACTGG AAGCTTCTTA 
AGTTATGAAA TCACAGATTT GAGCCTCACA GATCCAGAGT CATTTTCAGA ACAGACAGAG 
CATCTAGAAC AAGAATCTAC CAATAAGCAG GAAGAGACTG ATCCTCTTAG TATTGCAGTC 
CCTTCAGTTA TTTATCAGCA ACAGCATTCC TTAGGTGCTC ATAACTCTCT GTTGCCCATG 
GAGGAAGAAA GTACATCTGA TCATACACAT GTTCAGCAGA TCATGGACAA TGATGTAAAT 
GAAGCAAATT TGATACCTGA CAAAAGAGAC TTTCAGGTTC CAGCTGTGGA CCTTGACTTT 
CGAGAATTGG AACACATATT TCCACACTTG CATCGTCAGC TCTTTAAACC CTTAGAACCA 
CATCTAGATT TTGACTTATC ATCCCCTGGG ACATCTCAAG AAGACAGCGA CTTTTACCAG 
CAGAGCTCAG AATCTTCATC TGAAAAACAT GTTAAGGCAT TATCCACAGG CACAATCTGC 
TTCCCAGCAC TGACAGCCAA GTCACATTCT CCTAACCCAA GGCTGAACCA ACTTGATATT 
AACCTGGCTC ATGCAACAAC TGAGGGATCT GAACAGTCTT TTCAACAGCT TCGACCAGAA 
TTCTCTTCAC AGGAAAGCCA ACATGCTGAC CTACCCAGTA TTTATAGCAT TGAAGCAAGA 
GGTCCTTCCC AACGCATGGA AAACCAGAAC TATTCTGAAA TGTTACAAAA TAAGAAAAAA 
AGTCTCTCTC TCCAGCCCTC AACAGAGGAT TTGACTCCAG CTTGCAGTTC ATCTGATACT 
GCTCTGTTTG ATCAGTTACA CCTGCAGCAC AGCACTCCGT GTGCTTCTGT CTCTAGTGAG 
TGCTCAGTAA AACTGTTAGA AAGCAGAGAA GAAGTGCTGG GATTTGAAGA GCTGTCAAGA 
AGGGCAGTTA CAATGTCACA AAGACTCACT GAAGATGAAA ATGTAGTTCT ACCTATAAAC 
CCACATGTAG GAAGAGTGGA GAAGGAGGCT TCAGTTCAGG GGTCTAATCC ATTGAGTATT 
CAAAATGAAA AGCCAATTCA GAACTTCATT GAAACTGACA CTACAGAAGC TGTAGGAAAT 
GTATGTCAGC TAGCACAAGC AGAGCACATA TTAAAATCCT GTCCATTTCG GAGCCCTATT 
CCAATCTGGG AAACGGATAC TGGCTATGGT ATAATGGAAG AACCAGATCT CACTCTAGTG 
AGTAATAGTG ACATCAGCAT CACTGAAACA GATCTTGCAA ACTTAACCCT TGAAGACAGA 
GAAGACAATG AAGCACAGTT TTTTCAGGCA GGTGTCGTTC TGCCTACATC TTCAATGGAA 
ACCTCTGTTT GTGGAGCTGT ATCAGAGCCT TATGTGGACC AACCCACAGT GGCACCTTCA 
GCTACATCAG GTAGTTTACA AGAAGCATTT ATGACAAGAC AGACACTTAC AGAACGATCT 
TACCAGAGGC AGAGAGAGAT CTGGAATAAA ACTCGACTCC CTCAAACCAA AGTATCCAAA 
GAGAAGCTAC CTACAGGTTG CACAGGCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_030244414.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100274.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X11, mRNA.

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CloneID	OMu90203
Clone ID Related Accession (Same CDS sequence)	XM_030244415.1 , XM_030244414.1
Accession Version	XM_030244414.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGA TCCACTTGGC CCAAAACCAG GAGCGATTGA TGGAAGAACT CAAACAGCTG 
CAGAAAGAGG ACCTGGCCCG TAGGAGGCAG ACCGTGGCAC AGATGCCGCC ACAGATGCTG 
GAGTTGCCAT ACCGACGGAG TGAGATGAAG GAAGACAGGC AGCGAGAGCT GGAGTTTGCC 
TTTGAAGATA TGTACAATGC AGATAGGAAG GTGAAAGGAA ACCTGATTTT ACATCTTAAG 
CCAGAACCTT TGCCTACAAT CTCTGATCAG CTCCAAGATG AAGAACTGGA CCTTTCAATG 
GAACAAGAAA ATCAAGTGCC CTTGGCAGCA AAGATCCAAC AAATCCCCTC AAGAATTCTT 
TTTAAAAGAT TATTAAACAA GATTAGAAGT CAAAAATCTC TCTGGACAAT TAAATCTGTG 
TCTGAGGATG AAGGTGAGGT GACTTCGAGC ATCATTGAGA TTGAGAGTAA AGTGCCAAGC 
GTGGACTCGG GAGCCATTAT CACAGAGGAG AGAACAGCGG CGTCCTTTGA GCAGGAGCAA 
GTCACAGACA GTGATAGGCT GACGATAGAA TCTGGACCAC TGAGCAGTGA AGATAAGCCA 
TTGTACTACA AAGCGGGGAC TGGAAGGGAA CAAGCAATGG CAGTGTCTCC ACCTGCCACA 
GCTGTGGCTC AGAGTTCAGT CCTGCTTCAT CCTCAGGAAG AGGCAGTCAG AATTCGAATG 
TCATTAAGGC GCAAACAGAT AATGGAGATA GAAGAGCAGA AGCAGAAGCA GTTGGAATTA 
CTTGAACAAA TTGAGCAACA GAAGTTAAGG TTAGAAACTG ATTGTTTCCG GGCTCAACTG 
GAAGAGCAAA GAAAGCAAGC TGATCAGCCT GAGGTTTGCT GTGCTCCAAT GTCACATGCT 
ATGATTTCTG ATGAAGACTC TCACAGGCAG ATGATTCGTA ACTATCAACA TCAGCTTTTA 
CAACAAAACA GGTTACACAA GGAGACTGTT GAAACTGCCA GGAAACGATT ACTCGAATAC 
CAAACTGTAT TAAAAGAAAG ATCCCCATCC CTGTCAGCCT CAGCACTGGT ACCTGATTCT 
GTTGTGTCAG GGCCACCACA GCAATCCTAC AAGCCTGCTG CTGCTTCAGA CTCTTGGGAT 
CCAAGCCAGA GACTGAAACT AAGTCCTAGC AAATATCAAC CTGTGCAACC CTCACAGATC 
CCTGCATTAG AGCAGAGTCA TATTCAGGTA CCAAGACATG GTCACATTAC ACAGAGGCAA 
GGAAAAATGG CTGTATCAGA AATGTTAGGT AAGCAGCCCG TGGAGTCACA AGAACGTCAG 
TGGCAGTTCT CTCAGGTGGA AACACATCAG GGAGACTATG AGTTCGTCCT TAAAGATTCT 
CATTCACTTT CAAGGACTTT GTCTTATGTT AGGCCACAAA CATTACAAGA TGCTAGAGAA 
GTATCTAAAC CTCCTAGGGT AATAATTTGT CAAAGTCTAG ATTCCCAACA GATATCATCA 
GAGGATAGTG AAAATATATC TTCTAAGCCA AGTGAACCGT CTCCTTTTCT CCCACTGGTG 
CCTGAACGCC CTTTTACTAG TCTCCCAGTT AAATTCCATT CTGGAACAAT TCACAAACCC 
TTTACGACCA TAAACCAGTC TGTAATCAGT CAAATGCATG ATCAACCTTT GTCATCCTCA 
GAGACTATTA CAGCTCAGCA GGGTGACTTA AGATTTCTTC AAGAACAGTT AGAACTACAG 
AAGAAAGTTC TTCAGGCAAG ACAAGAGGCT CGGGAAAAGC TGCTTTTGTG CACACAGAAA 
GAACTGGGAC AGCAGACTGG CCTCCCAGTG TTCCTCCCTT CACCAGCTGG AAATATATTT 
TCTTCATTAC CCTCTGCCTC AGCTGAATCA GGAAACTTTC AGACATCTTC AACAAAAAGT 
GATGCCACTG TTTCCTCAGA CAATATGGAC AGGCTTTGGG ATTCTTCACA ACCCATCTCA 
TCACAGCAAA CTCATTTAGA ATTTCTCCAA GAGCAGTCCA GTGTTGAGAC GGATAACCTT 
CAGGCTAGGC GGGAAGCCCA GGAGGTCTTG TTTGCACATA CACAAAATAC GCTGGAAAAA 
ATTGTACGTT CTGAACAAGC TGGGTCTTCT TTGCCACATC AGGTAGCTCA GCAGTCATTT 
AGTTCACTAA CATTGGCTGA TACACAATCT AAAAAAATCC AGAAACAGCC TTTACCTGCA 
AACAAGAAAG GGCTACTCCC AAGCCAGTCT GAAGTCTCAA AAGCTCAGGA TGGATCTTCA 
GGTTTTCTAC AGCAGACCCT ACCTCTCCAG AACACTTTAA AGTTGCTTCA AGAGCAGCTG 
ACTAGACAGA GGAGTATGAT TCCGCCTAGA CGTGATGGTC AAGAGACATT ACTTTTATAT 
AAAGAAAGCT GTTCAGAAGA CAGTGAGGCT GGGCCAGTAG AATCACTCTC CTCCGTGGTT 
GTTCAGCATG CAGATGCTTC TCGTGCAGTT TCAGAAGTAC CTAAGCGGCT CCAGGACGTG 
TATTCATCTG AGGAAGAAAA TAGAGTTCTC TCCAGTCATT TGATCACCCA TGGCTTTCCA 
CAGCACAGCC TGCAAAGACA AGAACATTTT ACACCACTCC AGGAAGAGAC ACACATTCAG 
AGGCTTATAC TTGGTGCTAG AAAAAATAAT GAGGAATTTG CTCCCAAGCA AAATGAATTA 
GAAAAGGGGC TCTGCTCTCA ACAGACTGAT GCCTTGTCTT CTCCATCCCA AGTCACCGAC 
TGGGGAACAT CCCGGGGGTC TGTATCAGTC AGAAGTGATA GGACAGACCC CTTAAGGCAT 
TTTAAGATTC CAGCGTTTCG GGAAAGACTA GTTAGAGTTT CACAACATAC ATTCCCTCTC 
CAAGATAATT TGCAGGAACA CCAAGAATGG GTAGACACAG AAAAAGAGAG CTTTCAATCT 
AGTCCTCTAA CTCCAGAAAA TCCATCTTCT CAACAGACAG GTTTTTCGTC CTTCAAAGCC 
TCATTAAGAC TGCCATCGTG TGTGTCCTTG CCATCTGCTG ACTCTGGTAT CACACAGCAC 
CCTCTCTCAA CAGAGAGTGA CAGTAAAGTC AAGTCAAGCC ATCTTCAGAT ACCAGAACTG 
CAGCATAGAC TTTCGAAGAT ATCCCAGCTC ATCCCGCCTC AACAAGACAG TTTGAAGGCC 
CTTCAAGAAC AATTAGCTAC ACAAAGGGAA GCCATCATCC ACTCTAGACA AGAAGCTCAT 
GAAGAAACAC TAAGAGAATG GAAGGAAAAA ATATTCCCAG AGCAGGTTGG TCCATTTTCT 
CCCCTGATAC CACAACATTC ACTTGCTTCA TTCCCTGTTT CTGATACTGA AAGAGCACAA 
GAACTGTGTT CAACCAACAG TGACACCATA TCTTCAGGTT ATCCTGAGAT GCTGGAGTTG 
CCTGACAGGA CATTGGGTTT ATCACATACT GCTTTACCTC AGCAGAATAA TCTGACTGCA 
CATCCAGAAC ACCTTCATGC ACAAACAAAT TTTTTTCACT CTACTGAGAA AGCCCAAGAA 
GGGTTGGTGT TTCCCAGACC ATGTCAATTT GAAGAGATGT CTGCTGAGCA TTTTATCCAG 
CCTCAACATG ATGACTTAAA GGCACTTCAA CAGCAGTTAG ATATGCAAAG GGAAGCCATT 
AGATCTGGCC AGGAAATGCA AGAGAAAATG CTTTTGCAAA GGTTAAATAA ATTGGAGCAA 
AGGATATCTT CCAAACAAAT TAGCTCCTCT CTATTTTCAT CCCAGGTAGC ACTGCCCATT 
GCCAATTCTG ACGGGACCTT ACAGTCTTTC CCAACTAAAA GTAACGAGAC TGAGCTGCTT 
GGGTCCCAAG ATGAGTATCT GAGCTTTTCA CAGCCTCGTC TGCCTCTGCA GAATAACATG 
ACAGAGCAGT TGGACTTAGA AAAGGTATTT CATAAAGAAC TGCTTTTACA CAAACAAAAG 
AGCCAAAACA AAAGTGAATC TTCTGAGCAT TCCCTCCCTC CTTTGTTTCT GTCCAAGGAA 
ATAGAGCATC CATTCATTTC ACTACCTTTT GCAGAAAGTA AATCTAAAAG CATTTGTGAG 
TTGTATTTAT CTGACAAGAA GCATGCAGCT CCTAATGATG CTGTGATCCC AAGATTGCAG 
GACAGGCTTT TAAGCTGTTC CCAACCTGTC TTAACTCAGC AAGATAACAT GAGTCTTCAG 
AAGCAGCTGA ATCTACAAAG AGAAACTCTA CACTCTAGGC AAAAAGCCCA AGAAGAATTG 
CTTGTGCAGA GACAGACATC CTTACAGCAG CAAATCCAAA GACACAGAGA GACTTTGAAA 
AATTTCTTTA ATGTCAGTCA GGCAAGGAAT CCCACAGATG AGAATGACTT GGAAATGCAG 
AAGAGAGAAC AGCTCGGAGG GTGGTTTCCT CACACCCAAG GCCTTACCTG GGGAGACGCA 
GGTCAGGGGA GCGCTAATGG TGAGCAGCCA CGTGCAGATG TCCATGCTGA GCACAATGGT 
GAGAGTCTGG CCAAAGAACT GAGTGGTAGA GCCTCAAAGC CACCTGTCTC CAAAGTTAAA 
TGTGTCTTGG ACTTAAACCA GCATGAGCTT AGTACTATAC AGGAAGTAGA GTCACCAGCA 
AGTGGCAGGA TTTCCATGCC AGGTAAACAA GGAAAAGCAG AGTTTTATCA AGACCGAGAC 
CCATTAAGGG TCTCAGTAAG CAGAGAACAA AGTTTCTTGG AGAGCCCACT GGCACATGAT 
CCATTTGGTT GTCACCAACC ACCTGCCCAA GAGAACAGCA AAAGCCATGA CGACAATGCT 
GAAGCAGTTA AAGTCAAGAA ATCTGATGTT GAGGATCATG CAGTATTGAG TCATGCTGTC 
TCAAAAGAAG AAGCGTGTAC AAACCTGGGT CCACTTGGAA AGCCAGACGA TGAGGCTGAA 
ACACAAGAGA TTTCTCAGGA GCCATTGTCT TCAGTAACTG TTTCTACTGG AAGCTTCTTA 
AGTTATGAAA TCACAGATTT GAGCCTCACA GATCCAGAGT CATTTTCAGA ACAGACAGAG 
CATCTAGAAC AAGAATCTAC CAATAAGCAG GAAGAGACTG ATCCTCTTAG TATTGCAGTC 
CCTTCAGTTA TTTATCAGCA ACAGCATTCC TTAGGTGCTC ATAACTCTCT GTTGCCCATG 
GAGGAAGAAA GTACATCTGA TCATACACAT GTTCAGCAGA TCATGGACAA TGATGTAAAT 
GAAGCAAATT TGATACCTGA CAAAAGAGAC TTTCAGGTTC CAGCTGTGGA CCTTGACTTT 
CGAGAATTGG AACACATATT TCCACACTTG CATCGTCAGC TCTTTAAACC CTTAGAACCA 
CATCTAGATT TTGACTTATC ATCCCCTGGG ACATCTCAAG AAGACAGCGA CTTTTACCAG 
CAGAGCTCAG AATCTTCATC TGAAAAACAT GTTAAGGCAT TATCCACAGG CACAATCTGC 
TTCCCAGCAC TGACAGCCAA GTCACATTCT CCTAACCCAA GGCTGAACCA ACTTGATATT 
AACCTGGCTC ATGCAACAAC TGAGGGATCT GAACAGTCTT TTCAACAGCT TCGACCAGAA 
TTCTCTTCAC AGGAAAGCCA ACATGCTGAC CTACCCAGTA TTTATAGCAT TGAAGCAAGA 
GGTCCTTCCC AACGCATGGA AAACCAGAAC TATTCTGAAA TGTTACAAAA TAAGAAAAAA 
AGTCTCTCTC TCCAGCCCTC AACAGAGGAT TTGACTCCAG CTTGCAGTTC ATCTGATACT 
GCTCTGTTTG ATCAGTTACA CCTGCAGCAC AGCACTCCGT GTGCTTCTGT CTCTAGTGAG 
TGCTCAGTAA AACTGTTAGA AAGCAGAGAA GAAGTGCTGG GATTTGAAGA GCTGTCAAGA 
AGGGCAGTTA CAATGTCACA AAGACTCACT GAAGATGAAA ATGTAGTTCT ACCTATAAAC 
CCACATGTAG GAAGAGTGGA GAAGGAGGCT TCAGTTCAGG GGTCTAATCC ATTGAGTATT 
CAAAATGAAA AGCCAATTCA GAACTTCATT GAAACTGACA CTACAGAAGC TGTAGGAAAT 
GTATGTCAGC TAGCACAAGC AGAGCACATA TTAAAATCCT GTCCATTTCG GAGCCCTATT 
CCAATCTGGG AAACGGATAC TGGCTATGGT ATAATGGAAG AACCAGATCT CACTCTAGTG 
AGTAATAGTG ACATCAGCAT CACTGAAACA GATCTTGCAA ACTTAACCCT TGAAGACAGA 
GAAGACAATG AAGCACAGTT TTTTCAGGCA GGTGTCGTTC TGCCTACATC TTCAATGGAA 
ACCTCTGTTT GTGGAGCTGT ATCAGAGCCT TATGTGGACC AACCCACAGT GGCACCTTCA 
GCTACATCAG GTAGTTTACA AGAAGCATTT ATGACAAGAC AGACACTTAC AGAACGATCT 
TACCAGAGGC AGAGAGAGAT CTGGAATAAA ACTCGACTCC CTCAAACCAA AGTATCCAAA 
GAGAAGCTAC CTACAGGTTG CACAGGCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100274.1
CDS	868..7380
Translation	MKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_030244414.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244414.1

ORF Insert Sequence:

ATGAAAAAGA TCCACTTGGC CCAAAACCAG GAGCGATTGA TGGAAGAACT CAAACAGCTG 
CAGAAAGAGG ACCTGGCCCG TAGGAGGCAG ACCGTGGCAC AGATGCCGCC ACAGATGCTG 
GAGTTGCCAT ACCGACGGAG TGAGATGAAG GAAGACAGGC AGCGAGAGCT GGAGTTTGCC 
TTTGAAGATA TGTACAATGC AGATAGGAAG GTGAAAGGAA ACCTGATTTT ACATCTTAAG 
CCAGAACCTT TGCCTACAAT CTCTGATCAG CTCCAAGATG AAGAACTGGA CCTTTCAATG 
GAACAAGAAA ATCAAGTGCC CTTGGCAGCA AAGATCCAAC AAATCCCCTC AAGAATTCTT 
TTTAAAAGAT TATTAAACAA GATTAGAAGT CAAAAATCTC TCTGGACAAT TAAATCTGTG 
TCTGAGGATG AAGGTGAGGT GACTTCGAGC ATCATTGAGA TTGAGAGTAA AGTGCCAAGC 
GTGGACTCGG GAGCCATTAT CACAGAGGAG AGAACAGCGG CGTCCTTTGA GCAGGAGCAA 
GTCACAGACA GTGATAGGCT GACGATAGAA TCTGGACCAC TGAGCAGTGA AGATAAGCCA 
TTGTACTACA AAGCGGGGAC TGGAAGGGAA CAAGCAATGG CAGTGTCTCC ACCTGCCACA 
GCTGTGGCTC AGAGTTCAGT CCTGCTTCAT CCTCAGGAAG AGGCAGTCAG AATTCGAATG 
TCATTAAGGC GCAAACAGAT AATGGAGATA GAAGAGCAGA AGCAGAAGCA GTTGGAATTA 
CTTGAACAAA TTGAGCAACA GAAGTTAAGG TTAGAAACTG ATTGTTTCCG GGCTCAACTG 
GAAGAGCAAA GAAAGCAAGC TGATCAGCCT GAGGTTTGCT GTGCTCCAAT GTCACATGCT 
ATGATTTCTG ATGAAGACTC TCACAGGCAG ATGATTCGTA ACTATCAACA TCAGCTTTTA 
CAACAAAACA GGTTACACAA GGAGACTGTT GAAACTGCCA GGAAACGATT ACTCGAATAC 
CAAACTGTAT TAAAAGAAAG ATCCCCATCC CTGTCAGCCT CAGCACTGGT ACCTGATTCT 
GTTGTGTCAG GGCCACCACA GCAATCCTAC AAGCCTGCTG CTGCTTCAGA CTCTTGGGAT 
CCAAGCCAGA GACTGAAACT AAGTCCTAGC AAATATCAAC CTGTGCAACC CTCACAGATC 
CCTGCATTAG AGCAGAGTCA TATTCAGGTA CCAAGACATG GTCACATTAC ACAGAGGCAA 
GGAAAAATGG CTGTATCAGA AATGTTAGGT AAGCAGCCCG TGGAGTCACA AGAACGTCAG 
TGGCAGTTCT CTCAGGTGGA AACACATCAG GGAGACTATG AGTTCGTCCT TAAAGATTCT 
CATTCACTTT CAAGGACTTT GTCTTATGTT AGGCCACAAA CATTACAAGA TGCTAGAGAA 
GTATCTAAAC CTCCTAGGGT AATAATTTGT CAAAGTCTAG ATTCCCAACA GATATCATCA 
GAGGATAGTG AAAATATATC TTCTAAGCCA AGTGAACCGT CTCCTTTTCT CCCACTGGTG 
CCTGAACGCC CTTTTACTAG TCTCCCAGTT AAATTCCATT CTGGAACAAT TCACAAACCC 
TTTACGACCA TAAACCAGTC TGTAATCAGT CAAATGCATG ATCAACCTTT GTCATCCTCA 
GAGACTATTA CAGCTCAGCA GGGTGACTTA AGATTTCTTC AAGAACAGTT AGAACTACAG 
AAGAAAGTTC TTCAGGCAAG ACAAGAGGCT CGGGAAAAGC TGCTTTTGTG CACACAGAAA 
GAACTGGGAC AGCAGACTGG CCTCCCAGTG TTCCTCCCTT CACCAGCTGG AAATATATTT 
TCTTCATTAC CCTCTGCCTC AGCTGAATCA GGAAACTTTC AGACATCTTC AACAAAAAGT 
GATGCCACTG TTTCCTCAGA CAATATGGAC AGGCTTTGGG ATTCTTCACA ACCCATCTCA 
TCACAGCAAA CTCATTTAGA ATTTCTCCAA GAGCAGTCCA GTGTTGAGAC GGATAACCTT 
CAGGCTAGGC GGGAAGCCCA GGAGGTCTTG TTTGCACATA CACAAAATAC GCTGGAAAAA 
ATTGTACGTT CTGAACAAGC TGGGTCTTCT TTGCCACATC AGGTAGCTCA GCAGTCATTT 
AGTTCACTAA CATTGGCTGA TACACAATCT AAAAAAATCC AGAAACAGCC TTTACCTGCA 
AACAAGAAAG GGCTACTCCC AAGCCAGTCT GAAGTCTCAA AAGCTCAGGA TGGATCTTCA 
GGTTTTCTAC AGCAGACCCT ACCTCTCCAG AACACTTTAA AGTTGCTTCA AGAGCAGCTG 
ACTAGACAGA GGAGTATGAT TCCGCCTAGA CGTGATGGTC AAGAGACATT ACTTTTATAT 
AAAGAAAGCT GTTCAGAAGA CAGTGAGGCT GGGCCAGTAG AATCACTCTC CTCCGTGGTT 
GTTCAGCATG CAGATGCTTC TCGTGCAGTT TCAGAAGTAC CTAAGCGGCT CCAGGACGTG 
TATTCATCTG AGGAAGAAAA TAGAGTTCTC TCCAGTCATT TGATCACCCA TGGCTTTCCA 
CAGCACAGCC TGCAAAGACA AGAACATTTT ACACCACTCC AGGAAGAGAC ACACATTCAG 
AGGCTTATAC TTGGTGCTAG AAAAAATAAT GAGGAATTTG CTCCCAAGCA AAATGAATTA 
GAAAAGGGGC TCTGCTCTCA ACAGACTGAT GCCTTGTCTT CTCCATCCCA AGTCACCGAC 
TGGGGAACAT CCCGGGGGTC TGTATCAGTC AGAAGTGATA GGACAGACCC CTTAAGGCAT 
TTTAAGATTC CAGCGTTTCG GGAAAGACTA GTTAGAGTTT CACAACATAC ATTCCCTCTC 
CAAGATAATT TGCAGGAACA CCAAGAATGG GTAGACACAG AAAAAGAGAG CTTTCAATCT 
AGTCCTCTAA CTCCAGAAAA TCCATCTTCT CAACAGACAG GTTTTTCGTC CTTCAAAGCC 
TCATTAAGAC TGCCATCGTG TGTGTCCTTG CCATCTGCTG ACTCTGGTAT CACACAGCAC 
CCTCTCTCAA CAGAGAGTGA CAGTAAAGTC AAGTCAAGCC ATCTTCAGAT ACCAGAACTG 
CAGCATAGAC TTTCGAAGAT ATCCCAGCTC ATCCCGCCTC AACAAGACAG TTTGAAGGCC 
CTTCAAGAAC AATTAGCTAC ACAAAGGGAA GCCATCATCC ACTCTAGACA AGAAGCTCAT 
GAAGAAACAC TAAGAGAATG GAAGGAAAAA ATATTCCCAG AGCAGGTTGG TCCATTTTCT 
CCCCTGATAC CACAACATTC ACTTGCTTCA TTCCCTGTTT CTGATACTGA AAGAGCACAA 
GAACTGTGTT CAACCAACAG TGACACCATA TCTTCAGGTT ATCCTGAGAT GCTGGAGTTG 
CCTGACAGGA CATTGGGTTT ATCACATACT GCTTTACCTC AGCAGAATAA TCTGACTGCA 
CATCCAGAAC ACCTTCATGC ACAAACAAAT TTTTTTCACT CTACTGAGAA AGCCCAAGAA 
GGGTTGGTGT TTCCCAGACC ATGTCAATTT GAAGAGATGT CTGCTGAGCA TTTTATCCAG 
CCTCAACATG ATGACTTAAA GGCACTTCAA CAGCAGTTAG ATATGCAAAG GGAAGCCATT 
AGATCTGGCC AGGAAATGCA AGAGAAAATG CTTTTGCAAA GGTTAAATAA ATTGGAGCAA 
AGGATATCTT CCAAACAAAT TAGCTCCTCT CTATTTTCAT CCCAGGTAGC ACTGCCCATT 
GCCAATTCTG ACGGGACCTT ACAGTCTTTC CCAACTAAAA GTAACGAGAC TGAGCTGCTT 
GGGTCCCAAG ATGAGTATCT GAGCTTTTCA CAGCCTCGTC TGCCTCTGCA GAATAACATG 
ACAGAGCAGT TGGACTTAGA AAAGGTATTT CATAAAGAAC TGCTTTTACA CAAACAAAAG 
AGCCAAAACA AAAGTGAATC TTCTGAGCAT TCCCTCCCTC CTTTGTTTCT GTCCAAGGAA 
ATAGAGCATC CATTCATTTC ACTACCTTTT GCAGAAAGTA AATCTAAAAG CATTTGTGAG 
TTGTATTTAT CTGACAAGAA GCATGCAGCT CCTAATGATG CTGTGATCCC AAGATTGCAG 
GACAGGCTTT TAAGCTGTTC CCAACCTGTC TTAACTCAGC AAGATAACAT GAGTCTTCAG 
AAGCAGCTGA ATCTACAAAG AGAAACTCTA CACTCTAGGC AAAAAGCCCA AGAAGAATTG 
CTTGTGCAGA GACAGACATC CTTACAGCAG CAAATCCAAA GACACAGAGA GACTTTGAAA 
AATTTCTTTA ATGTCAGTCA GGCAAGGAAT CCCACAGATG AGAATGACTT GGAAATGCAG 
AAGAGAGAAC AGCTCGGAGG GTGGTTTCCT CACACCCAAG GCCTTACCTG GGGAGACGCA 
GGTCAGGGGA GCGCTAATGG TGAGCAGCCA CGTGCAGATG TCCATGCTGA GCACAATGGT 
GAGAGTCTGG CCAAAGAACT GAGTGGTAGA GCCTCAAAGC CACCTGTCTC CAAAGTTAAA 
TGTGTCTTGG ACTTAAACCA GCATGAGCTT AGTACTATAC AGGAAGTAGA GTCACCAGCA 
AGTGGCAGGA TTTCCATGCC AGGTAAACAA GGAAAAGCAG AGTTTTATCA AGACCGAGAC 
CCATTAAGGG TCTCAGTAAG CAGAGAACAA AGTTTCTTGG AGAGCCCACT GGCACATGAT 
CCATTTGGTT GTCACCAACC ACCTGCCCAA GAGAACAGCA AAAGCCATGA CGACAATGCT 
GAAGCAGTTA AAGTCAAGAA ATCTGATGTT GAGGATCATG CAGTATTGAG TCATGCTGTC 
TCAAAAGAAG AAGCGTGTAC AAACCTGGGT CCACTTGGAA AGCCAGACGA TGAGGCTGAA 
ACACAAGAGA TTTCTCAGGA GCCATTGTCT TCAGTAACTG TTTCTACTGG AAGCTTCTTA 
AGTTATGAAA TCACAGATTT GAGCCTCACA GATCCAGAGT CATTTTCAGA ACAGACAGAG 
CATCTAGAAC AAGAATCTAC CAATAAGCAG GAAGAGACTG ATCCTCTTAG TATTGCAGTC 
CCTTCAGTTA TTTATCAGCA ACAGCATTCC TTAGGTGCTC ATAACTCTCT GTTGCCCATG 
GAGGAAGAAA GTACATCTGA TCATACACAT GTTCAGCAGA TCATGGACAA TGATGTAAAT 
GAAGCAAATT TGATACCTGA CAAAAGAGAC TTTCAGGTTC CAGCTGTGGA CCTTGACTTT 
CGAGAATTGG AACACATATT TCCACACTTG CATCGTCAGC TCTTTAAACC CTTAGAACCA 
CATCTAGATT TTGACTTATC ATCCCCTGGG ACATCTCAAG AAGACAGCGA CTTTTACCAG 
CAGAGCTCAG AATCTTCATC TGAAAAACAT GTTAAGGCAT TATCCACAGG CACAATCTGC 
TTCCCAGCAC TGACAGCCAA GTCACATTCT CCTAACCCAA GGCTGAACCA ACTTGATATT 
AACCTGGCTC ATGCAACAAC TGAGGGATCT GAACAGTCTT TTCAACAGCT TCGACCAGAA 
TTCTCTTCAC AGGAAAGCCA ACATGCTGAC CTACCCAGTA TTTATAGCAT TGAAGCAAGA 
GGTCCTTCCC AACGCATGGA AAACCAGAAC TATTCTGAAA TGTTACAAAA TAAGAAAAAA 
AGTCTCTCTC TCCAGCCCTC AACAGAGGAT TTGACTCCAG CTTGCAGTTC ATCTGATACT 
GCTCTGTTTG ATCAGTTACA CCTGCAGCAC AGCACTCCGT GTGCTTCTGT CTCTAGTGAG 
TGCTCAGTAA AACTGTTAGA AAGCAGAGAA GAAGTGCTGG GATTTGAAGA GCTGTCAAGA 
AGGGCAGTTA CAATGTCACA AAGACTCACT GAAGATGAAA ATGTAGTTCT ACCTATAAAC 
CCACATGTAG GAAGAGTGGA GAAGGAGGCT TCAGTTCAGG GGTCTAATCC ATTGAGTATT 
CAAAATGAAA AGCCAATTCA GAACTTCATT GAAACTGACA CTACAGAAGC TGTAGGAAAT 
GTATGTCAGC TAGCACAAGC AGAGCACATA TTAAAATCCT GTCCATTTCG GAGCCCTATT 
CCAATCTGGG AAACGGATAC TGGCTATGGT ATAATGGAAG AACCAGATCT CACTCTAGTG 
AGTAATAGTG ACATCAGCAT CACTGAAACA GATCTTGCAA ACTTAACCCT TGAAGACAGA 
GAAGACAATG AAGCACAGTT TTTTCAGGCA GGTGTCGTTC TGCCTACATC TTCAATGGAA 
ACCTCTGTTT GTGGAGCTGT ATCAGAGCCT TATGTGGACC AACCCACAGT GGCACCTTCA 
GCTACATCAG GTAGTTTACA AGAAGCATTT ATGACAAGAC AGACACTTAC AGAACGATCT 
TACCAGAGGC AGAGAGAGAT CTGGAATAAA ACTCGACTCC CTCAAACCAA AGTATCCAAA 
GAGAAGCTAC CTACAGGTTG CACAGGCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( NM_176976.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_795950.2 Mus musculus centrosomal protein 295 (Cep295), mRNA.

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CloneID	OMu05807
Clone ID Related Accession (Same CDS sequence)	NM_176976.4
Accession Version	NM_176976.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY145307.1, CJ184811.1, CJ321667.1, BU920673.1, AC154296.2, CB320910.1, CD538842.1, BC118968.2, AK220270.1 and AV576800.1. On Nov 30, 2007 this sequence version replaced NM_176976.3. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGAGCTCAG AATCTTCATC TGAAAAACAT 
GTTAAGGCAT TATCCACAGG CACAATCTGC TTCCCAGCAC TGACAGCCAA GTCACATTCT 
CCTAACCCAA GGCTGAACCA ACTTGATATT AACCTGGCTC ATGCAACAAC TGAGGGATCT 
GAACAGTCTT TTCAACAGCT TCGACCAGAA TTCTCTTCAC AGGAAAGCCA ACATGCTGAC 
CTACCCAGTA TTTATAGCAT TGAAGCAAGA GGTCCTTCCC AACGCATGGA AAACCAGAAC 
TATTCTGAAA TGTTACAAAA TAAGAAAAAA AGTCTCTCTC TCCAGCCCTC AACAGAGGAT 
TTGACTCCAG CTTGCAGTTC ATCTGATACT GCTCTGTTTG ATCAGTTACA CCTGCAGCAC 
AGCACTCCGT GTGCTTCTGT CTCTAGTGAG TGCTCAGTAA AACTGTTAGA AAGCAGAGAA 
GAAGTGCTGG GATTTGAAGA GCTGTCAAGA AGGGCAGTTA CAATGTCACA AAGACTCACT 
GAAGATGAAA ATGTAGTTCT ACCTATAAAC CCACATGTAG GAAGAGTGGA GAAGGAGGCT 
TCAGTTCAGG GGTCTAATCC ATTGAGTATT CAAAATGAAA AGCCAATTCA GAACTTCATT 
GAAACTGACA CTACAGAAGC TGTAGGAAAT GTATGTCAGC TAGCACAAGC AGAGCACATA 
TTAAAATCCT GTCCATTTCG GAGCCCTATT CCAATCTGGG AAACGGATAC TGGCTATGGT 
ATAATGGAAG AACCAGATCT CACTCTAGTG AGTAATAGTG ACATCAGCAT CACTGAAACA 
GATCTTGCAA ACTTAACCCT TGAAGACAGA GAAGACAATG AAGCACAGTT TTTTCAGGCA 
GGTGTCGTTC TGCCTACATC TTCAATGGAA ACCTCTGTTT GTGGAGCTGT ATCAGAGCCT 
TATGTGGACC AACCCACAGT GGCACCTTCA GCTACATCAG GTAGTTTACA AGAAGCATTT 
ATGACAAGAC AGACACTTAC AGAACGATCT TACCAGAGGC AGAGAGAGAT CTGGAATAAA 
ACTCGACTCC CTCAAACCAA AGTATCCAAA GAGAAGCTAC CTACAGGTTG CACAGGCAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_795950.2
CDS	46..7188
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	NM_176976.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176976.4

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGAGCTCAG AATCTTCATC TGAAAAACAT 
GTTAAGGCAT TATCCACAGG CACAATCTGC TTCCCAGCAC TGACAGCCAA GTCACATTCT 
CCTAACCCAA GGCTGAACCA ACTTGATATT AACCTGGCTC ATGCAACAAC TGAGGGATCT 
GAACAGTCTT TTCAACAGCT TCGACCAGAA TTCTCTTCAC AGGAAAGCCA ACATGCTGAC 
CTACCCAGTA TTTATAGCAT TGAAGCAAGA GGTCCTTCCC AACGCATGGA AAACCAGAAC 
TATTCTGAAA TGTTACAAAA TAAGAAAAAA AGTCTCTCTC TCCAGCCCTC AACAGAGGAT 
TTGACTCCAG CTTGCAGTTC ATCTGATACT GCTCTGTTTG ATCAGTTACA CCTGCAGCAC 
AGCACTCCGT GTGCTTCTGT CTCTAGTGAG TGCTCAGTAA AACTGTTAGA AAGCAGAGAA 
GAAGTGCTGG GATTTGAAGA GCTGTCAAGA AGGGCAGTTA CAATGTCACA AAGACTCACT 
GAAGATGAAA ATGTAGTTCT ACCTATAAAC CCACATGTAG GAAGAGTGGA GAAGGAGGCT 
TCAGTTCAGG GGTCTAATCC ATTGAGTATT CAAAATGAAA AGCCAATTCA GAACTTCATT 
GAAACTGACA CTACAGAAGC TGTAGGAAAT GTATGTCAGC TAGCACAAGC AGAGCACATA 
TTAAAATCCT GTCCATTTCG GAGCCCTATT CCAATCTGGG AAACGGATAC TGGCTATGGT 
ATAATGGAAG AACCAGATCT CACTCTAGTG AGTAATAGTG ACATCAGCAT CACTGAAACA 
GATCTTGCAA ACTTAACCCT TGAAGACAGA GAAGACAATG AAGCACAGTT TTTTCAGGCA 
GGTGTCGTTC TGCCTACATC TTCAATGGAA ACCTCTGTTT GTGGAGCTGT ATCAGAGCCT 
TATGTGGACC AACCCACAGT GGCACCTTCA GCTACATCAG GTAGTTTACA AGAAGCATTT 
ATGACAAGAC AGACACTTAC AGAACGATCT TACCAGAGGC AGAGAGAGAT CTGGAATAAA 
ACTCGACTCC CTCAAACCAA AGTATCCAAA GAGAAGCTAC CTACAGGTTG CACAGGCAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_006510409.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510472.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X3, mRNA.

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CloneID	OMu42262
Clone ID Related Accession (Same CDS sequence)	XM_006510409.1 , XM_006510409.2
Accession Version	XM_006510409.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG AGTCATTTTC AGAACAGACA GAGCATCTAG AACAAGAATC TACCAATAAG 
CAGGAAGAGA CTGATCCTCT TAGTATTGCA GTCCCTTCAG TTATTTATCA GCAACAGCAT 
TCCTTAGGTG CTCATAACTC TCTGTTGCCC ATGGAGGAAG AAAGTACATC TGATCATACA 
CATGTTCAGC AGATCATGGA CAATGATGTA AATGAAGCAA ATTTGATACC TGACAAAAGA 
GACTTTCAGG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGCAGAGCT CAGAATCTTC ATCTGAAAAA 
CATGTTAAGG CATTATCCAC AGGCACAATC TGCTTCCCAG CACTGACAGC CAAGTCACAT 
TCTCCTAACC CAAGGCTGAA CCAACTTGAT ATTAACCTGG CTCATGCAAC AACTGAGGGA 
TCTGAACAGT CTTTTCAACA GCTTCGACCA GAATTCTCTT CACAGGAAAG CCAACATGCT 
GACCTACCCA GTATTTATAG CATTGAAGCA AGAGGTCCTT CCCAACGCAT GGAAAACCAG 
AACTATTCTG AAATGTTACA AAATAAGAAA AAAAGTCTCT CTCTCCAGCC CTCAACAGAG 
GATTTGACTC CAGCTTGCAG TTCATCTGAT ACTGCTCTGT TTGATCAGTT ACACCTGCAG 
CACAGCACTC CGTGTGCTTC TGTCTCTAGT GAGTGCTCAG TAAAACTGTT AGAAAGCAGA 
GAAGAAGTGC TGGGATTTGA AGAGCTGTCA AGAAGGGCAG TTACAATGTC ACAAAGACTC 
ACTGAAGATG AAAATGTAGT TCTACCTATA AACCCACATG TAGGAAGAGT GGAGAAGGAG 
GCTTCAGTTC AGGGGTCTAA TCCATTGAGT ATTCAAAATG AAAAGCCAAT TCAGAACTTC 
ATTGAAACTG ACACTACAGA AGCTGTAGGA AATGTATGTC AGCTAGCACA AGCAGAGCAC 
ATATTAAAAT CCTGTCCATT TCGGAGCCCT ATTCCAATCT GGGAAACGGA TACTGGCTAT 
GGTATAATGG AAGAACCAGA TCTCACTCTA GTGAGTAATA GTGACATCAG CATCACTGAA 
ACAGATCTTG CAAACTTAAC CCTTGAAGAC AGAGAAGACA ATGAAGCACA GTTTTTTCAG 
GCAGGTGTCG TTCTGCCTAC ATCTTCAATG GAAACCTCTG TTTGTGGAGC TGTATCAGAG 
CCTTATGTGG ACCAACCCAC AGTGGCACCT TCAGCTACAT CAGGTAGTTT ACAAGAAGCA 
TTTATGACAA GACAGACACT TACAGAACGA TCTTACCAGA GGCAGAGAGA GATCTGGAAT 
AAAACTCGAC TCCCTCAAAC CAAAGTATCC AAAGAGAAGC TACCTACAGG TTGCACAGGC 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510472.1
CDS	77..7462
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_006510409.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510409.1

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGAAAAG CAGAGTTTTA TCAAGACCGA GACCCATTAA GGGTCTCAGT AAGCAGAGAA 
CAAAGTTTCT TGGAGAGCCC ACTGGCACAT GATCCATTTG GTTGTCACCA ACCACCTGCC 
CAAGAGAACA GCAAAAGCCA TGACGACAAT GCTGAAGCAG TTAAAGTCAA GAAATCTGAT 
GTTGAGGATC ATGCAGTATT GAGTCATGCT GTCTCAAAAG AAGAAGCGTG TACAAACCTG 
GGTCCACTTG GAAAGCCAGA CGATGAGGCT GAAACACAAG AGATTTCTCA GGAGCCATTG 
TCTTCAGTAA CTGTTTCTAC TGGAAGCTTC TTAAGTTATG AAATCACAGA TTTGAGCCTC 
ACAGATCCAG AGTCATTTTC AGAACAGACA GAGCATCTAG AACAAGAATC TACCAATAAG 
CAGGAAGAGA CTGATCCTCT TAGTATTGCA GTCCCTTCAG TTATTTATCA GCAACAGCAT 
TCCTTAGGTG CTCATAACTC TCTGTTGCCC ATGGAGGAAG AAAGTACATC TGATCATACA 
CATGTTCAGC AGATCATGGA CAATGATGTA AATGAAGCAA ATTTGATACC TGACAAAAGA 
GACTTTCAGG TTCCAGCTGT GGACCTTGAC TTTCGAGAAT TGGAACACAT ATTTCCACAC 
TTGCATCGTC AGCTCTTTAA ACCCTTAGAA CCACATCTAG ATTTTGACTT ATCATCCCCT 
GGGACATCTC AAGAAGACAG CGACTTTTAC CAGCAGAGCT CAGAATCTTC ATCTGAAAAA 
CATGTTAAGG CATTATCCAC AGGCACAATC TGCTTCCCAG CACTGACAGC CAAGTCACAT 
TCTCCTAACC CAAGGCTGAA CCAACTTGAT ATTAACCTGG CTCATGCAAC AACTGAGGGA 
TCTGAACAGT CTTTTCAACA GCTTCGACCA GAATTCTCTT CACAGGAAAG CCAACATGCT 
GACCTACCCA GTATTTATAG CATTGAAGCA AGAGGTCCTT CCCAACGCAT GGAAAACCAG 
AACTATTCTG AAATGTTACA AAATAAGAAA AAAAGTCTCT CTCTCCAGCC CTCAACAGAG 
GATTTGACTC CAGCTTGCAG TTCATCTGAT ACTGCTCTGT TTGATCAGTT ACACCTGCAG 
CACAGCACTC CGTGTGCTTC TGTCTCTAGT GAGTGCTCAG TAAAACTGTT AGAAAGCAGA 
GAAGAAGTGC TGGGATTTGA AGAGCTGTCA AGAAGGGCAG TTACAATGTC ACAAAGACTC 
ACTGAAGATG AAAATGTAGT TCTACCTATA AACCCACATG TAGGAAGAGT GGAGAAGGAG 
GCTTCAGTTC AGGGGTCTAA TCCATTGAGT ATTCAAAATG AAAAGCCAAT TCAGAACTTC 
ATTGAAACTG ACACTACAGA AGCTGTAGGA AATGTATGTC AGCTAGCACA AGCAGAGCAC 
ATATTAAAAT CCTGTCCATT TCGGAGCCCT ATTCCAATCT GGGAAACGGA TACTGGCTAT 
GGTATAATGG AAGAACCAGA TCTCACTCTA GTGAGTAATA GTGACATCAG CATCACTGAA 
ACAGATCTTG CAAACTTAAC CCTTGAAGAC AGAGAAGACA ATGAAGCACA GTTTTTTCAG 
GCAGGTGTCG TTCTGCCTAC ATCTTCAATG GAAACCTCTG TTTGTGGAGC TGTATCAGAG 
CCTTATGTGG ACCAACCCAC AGTGGCACCT TCAGCTACAT CAGGTAGTTT ACAAGAAGCA 
TTTATGACAA GACAGACACT TACAGAACGA TCTTACCAGA GGCAGAGAGA GATCTGGAAT 
AAAACTCGAC TCCCTCAAAC CAAAGTATCC AAAGAGAAGC TACCTACAGG TTGCACAGGC 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_006510411.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510474.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X6, mRNA.

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CloneID	OMu42264
Clone ID Related Accession (Same CDS sequence)	XM_006510411.1 , XM_006510411.2
Accession Version	XM_006510411.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GAAAAGCAGA GTTTTATCAA 
GACCGAGACC CATTAAGGGT CTCAGTAAGC AGAGAACAAA GTTTCTTGGA GAGCCCACTG 
GCACATGATC CATTTGGTTG TCACCAACCA CCTGCCCAAG AGAACAGCAA AAGCCATGAC 
GACAATGCTG AAGCAGTTAA AGTCAAGAAA TCTGATGTTG AGGATCATGC AGTATTGAGT 
CATGCTGTCT CAAAAGAAGA AGCGTGTACA AACCTGGGTC CACTTGGAAA GCCAGACGAT 
GAGGCTGAAA CACAAGAGAT TTCTCAGGAG CCATTGTCTT CAGTAACTGT TTCTACTGGA 
AGCTTCTTAA GTTATGAAAT CACAGATTTG AGCCTCACAG ATCCAGAGTC ATTTTCAGAA 
CAGACAGAGC ATCTAGAACA AGAATCTACC AATAAGCAGG AAGAGACTGA TCCTCTTAGT 
ATTGCAGTCC CTTCAGTTAT TTATCAGCAA CAGCATTCCT TAGGTGCTCA TAACTCTCTG 
TTGCCCATGG AGGAAGAAAG TACATCTGAT CATACACATG TTCAGCAGAT CATGGACAAT 
GATGTAAATG AAGCAAATTT GATACCTGAC AAAAGAGACT TTCAGGTTCC AGCTGTGGAC 
CTTGACTTTC GAGAATTGGA ACACATATTT CCACACTTGC ATCGTCAGCT CTTTAAACCC 
TTAGAACCAC ATCTAGATTT TGACTTATCA TCCCCTGGGA CATCTCAAGA AGACAGCGAC 
TTTTACCAGC AGAGCTCAGA ATCTTCATCT GAAAAACATG TTAAGGCATT ATCCACAGGC 
ACAATCTGCT TCCCAGCACT GACAGCCAAG TCACATTCTC CTAACCCAAG GCTGAACCAA 
CTTGATATTA ACCTGGCTCA TGCAACAACT GAGGGATCTG AACAGTCTTT TCAACAGCTT 
CGACCAGAAT TCTCTTCACA GGAAAGCCAA CATGCTGACC TACCCAGTAT TTATAGCATT 
GAAGCAAGAG GTCCTTCCCA ACGCATGGAA AACCAGAACT ATTCTGAAAT GTTACAAAAT 
AAGAAAAAAA GTCTCTCTCT CCAGCCCTCA ACAGAGGATT TGACTCCAGC TTGCAGTTCA 
TCTGATACTG CTCTGTTTGA TCAGTTACAC CTGCAGCACA GCACTCCGTG TGCTTCTGTC 
TCTAGTGAGT GCTCAGTAAA ACTGTTAGAA AGCAGAGAAG AAGTGCTGGG ATTTGAAGAG 
CTGTCAAGAA GGGCAGTTAC AATGTCACAA AGACTCACTG AAGATGAAAA TGTAGTTCTA 
CCTATAAACC CACATGTAGG AAGAGTGGAG AAGGAGGCTT CAGTTCAGGG GTCTAATCCA 
TTGAGTATTC AAAATGAAAA GCCAATTCAG AACTTCATTG AAACTGACAC TACAGAAGCT 
GTAGGAAATG TATGTCAGCT AGCACAAGCA GAGCACATAT TAAAATCCTG TCCATTTCGG 
AGCCCTATTC CAATCTGGGA AACGGATACT GGCTATGGTA TAATGGAAGA ACCAGATCTC 
ACTCTAGTGA GTAATAGTGA CATCAGCATC ACTGAAACAG ATCTTGCAAA CTTAACCCTT 
GAAGACAGAG AAGACAATGA AGCACAGTTT TTTCAGGCAG GTGTCGTTCT GCCTACATCT 
TCAATGGAAA CCTCTGTTTG TGGAGCTGTA TCAGAGCCTT ATGTGGACCA ACCCACAGTG 
GCACCTTCAG CTACATCAGG TAGTTTACAA GAAGCATTTA TGACAAGACA GACACTTACA 
GAACGATCTT ACCAGAGGCA GAGAGAGATC TGGAATAAAA CTCGACTCCC TCAAACCAAA 
GTATCCAAAG AGAAGCTACC TACAGGTTGC ACAGGCAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510474.1
CDS	77..7318
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_006510411.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510411.1

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GAAAAGCAGA GTTTTATCAA 
GACCGAGACC CATTAAGGGT CTCAGTAAGC AGAGAACAAA GTTTCTTGGA GAGCCCACTG 
GCACATGATC CATTTGGTTG TCACCAACCA CCTGCCCAAG AGAACAGCAA AAGCCATGAC 
GACAATGCTG AAGCAGTTAA AGTCAAGAAA TCTGATGTTG AGGATCATGC AGTATTGAGT 
CATGCTGTCT CAAAAGAAGA AGCGTGTACA AACCTGGGTC CACTTGGAAA GCCAGACGAT 
GAGGCTGAAA CACAAGAGAT TTCTCAGGAG CCATTGTCTT CAGTAACTGT TTCTACTGGA 
AGCTTCTTAA GTTATGAAAT CACAGATTTG AGCCTCACAG ATCCAGAGTC ATTTTCAGAA 
CAGACAGAGC ATCTAGAACA AGAATCTACC AATAAGCAGG AAGAGACTGA TCCTCTTAGT 
ATTGCAGTCC CTTCAGTTAT TTATCAGCAA CAGCATTCCT TAGGTGCTCA TAACTCTCTG 
TTGCCCATGG AGGAAGAAAG TACATCTGAT CATACACATG TTCAGCAGAT CATGGACAAT 
GATGTAAATG AAGCAAATTT GATACCTGAC AAAAGAGACT TTCAGGTTCC AGCTGTGGAC 
CTTGACTTTC GAGAATTGGA ACACATATTT CCACACTTGC ATCGTCAGCT CTTTAAACCC 
TTAGAACCAC ATCTAGATTT TGACTTATCA TCCCCTGGGA CATCTCAAGA AGACAGCGAC 
TTTTACCAGC AGAGCTCAGA ATCTTCATCT GAAAAACATG TTAAGGCATT ATCCACAGGC 
ACAATCTGCT TCCCAGCACT GACAGCCAAG TCACATTCTC CTAACCCAAG GCTGAACCAA 
CTTGATATTA ACCTGGCTCA TGCAACAACT GAGGGATCTG AACAGTCTTT TCAACAGCTT 
CGACCAGAAT TCTCTTCACA GGAAAGCCAA CATGCTGACC TACCCAGTAT TTATAGCATT 
GAAGCAAGAG GTCCTTCCCA ACGCATGGAA AACCAGAACT ATTCTGAAAT GTTACAAAAT 
AAGAAAAAAA GTCTCTCTCT CCAGCCCTCA ACAGAGGATT TGACTCCAGC TTGCAGTTCA 
TCTGATACTG CTCTGTTTGA TCAGTTACAC CTGCAGCACA GCACTCCGTG TGCTTCTGTC 
TCTAGTGAGT GCTCAGTAAA ACTGTTAGAA AGCAGAGAAG AAGTGCTGGG ATTTGAAGAG 
CTGTCAAGAA GGGCAGTTAC AATGTCACAA AGACTCACTG AAGATGAAAA TGTAGTTCTA 
CCTATAAACC CACATGTAGG AAGAGTGGAG AAGGAGGCTT CAGTTCAGGG GTCTAATCCA 
TTGAGTATTC AAAATGAAAA GCCAATTCAG AACTTCATTG AAACTGACAC TACAGAAGCT 
GTAGGAAATG TATGTCAGCT AGCACAAGCA GAGCACATAT TAAAATCCTG TCCATTTCGG 
AGCCCTATTC CAATCTGGGA AACGGATACT GGCTATGGTA TAATGGAAGA ACCAGATCTC 
ACTCTAGTGA GTAATAGTGA CATCAGCATC ACTGAAACAG ATCTTGCAAA CTTAACCCTT 
GAAGACAGAG AAGACAATGA AGCACAGTTT TTTCAGGCAG GTGTCGTTCT GCCTACATCT 
TCAATGGAAA CCTCTGTTTG TGGAGCTGTA TCAGAGCCTT ATGTGGACCA ACCCACAGTG 
GCACCTTCAG CTACATCAGG TAGTTTACAA GAAGCATTTA TGACAAGACA GACACTTACA 
GAACGATCTT ACCAGAGGCA GAGAGAGATC TGGAATAAAA CTCGACTCCC TCAAACCAAA 
GTATCCAAAG AGAAGCTACC TACAGGTTGC ACAGGCAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_006510414.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510477.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X11, mRNA.

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CloneID	OMu42267
Clone ID Related Accession (Same CDS sequence)	XM_006510414.1 , XM_006510414.2
Accession Version	XM_006510414.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGCA AGGAATCCCA CAGATGAGAA TGACTTGGAA 
ATGCAGAAGA GAGAACAGCT CGGAGGGTGG TTTCCTCACA CCCAAGGCCT TACCTGGGGA 
GACGCAGGTC AGGGGAGCGC TAATGGTGAG CAGCCACGTG CAGATGTCCA TGCTGAGCAC 
AATGGTGAGA GTCTGGCCAA AGAACTGAGT GGTAGAGCCT CAAAGCCACC TGTCTCCAAA 
GTTAAATGTG TCTTGGACTT AAACCAGCAT GAGCTTAGTA CTATACAGGA AGTAGAGTCA 
CCAGCAAGTG GCAGGATTTC CATGCCAGGA AAAGCAGAGT TTTATCAAGA CCGAGACCCA 
TTAAGGGTCT CAGTAAGCAG AGAACAAAGT TTCTTGGAGA GCCCACTGGC ACATGATCCA 
TTTGGTTGTC ACCAACCACC TGCCCAAGAG AACAGCAAAA GCCATGACGA CAATGCTGAA 
GCAGTTAAAG TCAAGAAATC TGATGTTGAG GATCATGCAG TATTGAGTCA TGCTGTCTCA 
AAAGAAGAAG CGTGTACAAA CCTGGGTCCA CTTGGAAAGC CAGACGATGA GGCTGAAACA 
CAAGAGATTT CTCAGGAGCC ATTGTCTTCA GTAACTGTTT CTACTGGAAG CTTCTTAAGT 
TATGAAATCA CAGATTTGAG CCTCACAGAT CCAGTTCCAG CTGTGGACCT TGACTTTCGA 
GAATTGGAAC ACATATTTCC ACACTTGCAT CGTCAGCTCT TTAAACCCTT AGAACCACAT 
CTAGATTTTG ACTTATCATC CCCTGGGACA TCTCAAGAAG ACAGCGACTT TTACCAGAGC 
TCAGAATCTT CATCTGAAAA ACATGTTAAG GCATTATCCA CAGGCACAAT CTGCTTCCCA 
GCACTGACAG CCAAGTCACA TTCTCCTAAC CCAAGGCTGA ACCAACTTGA TATTAACCTG 
GCTCATGCAA CAACTGAGGG ATCTGAACAG TCTTTTCAAC AGCTTCGACC AGAATTCTCT 
TCACAGGAAA GCCAACATGC TGACCTACCC AGTATTTATA GCATTGAAGC AAGAGGTCCT 
TCCCAACGCA TGGAAAACCA GAACTATTCT GAAATGTTAC AAAATAAGAA AAAAAGTCTC 
TCTCTCCAGC CCTCAACAGA GGATTTGACT CCAGCTTGCA GTTCATCTGA TACTGCTCTG 
TTTGATCAGT TACACCTGCA GCACAGCACT CCGTGTGCTT CTGTCTCTAG TGAGTGCTCA 
GTAAAACTGT TAGAAAGCAG AGAAGAAGTG CTGGGATTTG AAGAGCTGTC AAGAAGGGCA 
GTTACAATGT CACAAAGACT CACTGAAGAT GAAAATGTAG TTCTACCTAT AAACCCACAT 
GTAGGAAGAG TGGAGAAGGA GGCTTCAGTT CAGGGGTCTA ATCCATTGAG TATTCAAAAT 
GAAAAGCCAA TTCAGAACTT CATTGAAACT GACACTACAG AAGCTGTAGG AAATGTATGT 
CAGCTAGCAC AAGCAGAGCA CATATTAAAA TCCTGTCCAT TTCGGAGCCC TATTCCAATC 
TGGGAAACGG ATACTGGCTA TGGTATAATG GAAGAACCAG ATCTCACTCT AGTGAGTAAT 
AGTGACATCA GCATCACTGA AACAGATCTT GCAAACTTAA CCCTTGAAGA CAGAGAAGAC 
AATGAAGCAC AGTTTTTTCA GGCAGGTGTC GTTCTGCCTA CATCTTCAAT GGAAACCTCT 
GTTTGTGGAG CTGTATCAGA GCCTTATGTG GACCAACCCA CAGTGGCACC TTCAGCTACA 
TCAGGTAGTT TACAAGAAGC ATTTATGACA AGACAGACAC TTACAGAACG ATCTTACCAG 
AGGCAGAGAG AGATCTGGAA TAAAACTCGA CTCCCTCAAA CCAAAGTATC CAAAGAGAAG 
CTACCTACAG GTTGCACAGG CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510477.1
CDS	78..6164
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_006510414.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510414.1

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGCA AGGAATCCCA CAGATGAGAA TGACTTGGAA 
ATGCAGAAGA GAGAACAGCT CGGAGGGTGG TTTCCTCACA CCCAAGGCCT TACCTGGGGA 
GACGCAGGTC AGGGGAGCGC TAATGGTGAG CAGCCACGTG CAGATGTCCA TGCTGAGCAC 
AATGGTGAGA GTCTGGCCAA AGAACTGAGT GGTAGAGCCT CAAAGCCACC TGTCTCCAAA 
GTTAAATGTG TCTTGGACTT AAACCAGCAT GAGCTTAGTA CTATACAGGA AGTAGAGTCA 
CCAGCAAGTG GCAGGATTTC CATGCCAGGA AAAGCAGAGT TTTATCAAGA CCGAGACCCA 
TTAAGGGTCT CAGTAAGCAG AGAACAAAGT TTCTTGGAGA GCCCACTGGC ACATGATCCA 
TTTGGTTGTC ACCAACCACC TGCCCAAGAG AACAGCAAAA GCCATGACGA CAATGCTGAA 
GCAGTTAAAG TCAAGAAATC TGATGTTGAG GATCATGCAG TATTGAGTCA TGCTGTCTCA 
AAAGAAGAAG CGTGTACAAA CCTGGGTCCA CTTGGAAAGC CAGACGATGA GGCTGAAACA 
CAAGAGATTT CTCAGGAGCC ATTGTCTTCA GTAACTGTTT CTACTGGAAG CTTCTTAAGT 
TATGAAATCA CAGATTTGAG CCTCACAGAT CCAGTTCCAG CTGTGGACCT TGACTTTCGA 
GAATTGGAAC ACATATTTCC ACACTTGCAT CGTCAGCTCT TTAAACCCTT AGAACCACAT 
CTAGATTTTG ACTTATCATC CCCTGGGACA TCTCAAGAAG ACAGCGACTT TTACCAGAGC 
TCAGAATCTT CATCTGAAAA ACATGTTAAG GCATTATCCA CAGGCACAAT CTGCTTCCCA 
GCACTGACAG CCAAGTCACA TTCTCCTAAC CCAAGGCTGA ACCAACTTGA TATTAACCTG 
GCTCATGCAA CAACTGAGGG ATCTGAACAG TCTTTTCAAC AGCTTCGACC AGAATTCTCT 
TCACAGGAAA GCCAACATGC TGACCTACCC AGTATTTATA GCATTGAAGC AAGAGGTCCT 
TCCCAACGCA TGGAAAACCA GAACTATTCT GAAATGTTAC AAAATAAGAA AAAAAGTCTC 
TCTCTCCAGC CCTCAACAGA GGATTTGACT CCAGCTTGCA GTTCATCTGA TACTGCTCTG 
TTTGATCAGT TACACCTGCA GCACAGCACT CCGTGTGCTT CTGTCTCTAG TGAGTGCTCA 
GTAAAACTGT TAGAAAGCAG AGAAGAAGTG CTGGGATTTG AAGAGCTGTC AAGAAGGGCA 
GTTACAATGT CACAAAGACT CACTGAAGAT GAAAATGTAG TTCTACCTAT AAACCCACAT 
GTAGGAAGAG TGGAGAAGGA GGCTTCAGTT CAGGGGTCTA ATCCATTGAG TATTCAAAAT 
GAAAAGCCAA TTCAGAACTT CATTGAAACT GACACTACAG AAGCTGTAGG AAATGTATGT 
CAGCTAGCAC AAGCAGAGCA CATATTAAAA TCCTGTCCAT TTCGGAGCCC TATTCCAATC 
TGGGAAACGG ATACTGGCTA TGGTATAATG GAAGAACCAG ATCTCACTCT AGTGAGTAAT 
AGTGACATCA GCATCACTGA AACAGATCTT GCAAACTTAA CCCTTGAAGA CAGAGAAGAC 
AATGAAGCAC AGTTTTTTCA GGCAGGTGTC GTTCTGCCTA CATCTTCAAT GGAAACCTCT 
GTTTGTGGAG CTGTATCAGA GCCTTATGTG GACCAACCCA CAGTGGCACC TTCAGCTACA 
TCAGGTAGTT TACAAGAAGC ATTTATGACA AGACAGACAC TTACAGAACG ATCTTACCAG 
AGGCAGAGAG AGATCTGGAA TAAAACTCGA CTCCCTCAAA CCAAAGTATC CAAAGAGAAG 
CTACCTACAG GTTGCACAGG CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_011242547.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240849.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X2, mRNA.

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CloneID	OMu74558
Clone ID Related Accession (Same CDS sequence)	XM_011242547.1 , XM_011242547.2
Accession Version	XM_011242547.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGTAAAC AAGGAAAAGC AGAGTTTTAT CAAGACCGAG ACCCATTAAG GGTCTCAGTA 
AGCAGAGAAC AAAGTTTCTT GGAGAGCCCA CTGGCACATG ATCCATTTGG TTGTCACCAA 
CCACCTGCCC AAGAGAACAG CAAAAGCCAT GACGACAATG CTGAAGCAGT TAAAGTCAAG 
AAATCTGATG TTGAGGATCA TGCAGTATTG AGTCATGCTG TCTCAAAAGA AGAAGCGTGT 
ACAAACCTGG GTCCACTTGG AAAGCCAGAC GATGAGGCTG AAACACAAGA GATTTCTCAG 
GAGCCATTGT CTTCAGTAAC TGTTTCTACT GGAAGCTTCT TAAGTTATGA AATCACAGAT 
TTGAGCCTCA CAGATCCAGA GTCATTTTCA GAACAGACAG AGCATCTAGA ACAAGAATCT 
ACCAATAAGC AGGAAGAGAC TGATCCTCTT AGTATTGCAG TCCCTTCAGT TATTTATCAG 
CAACAGCATT CCTTAGGTGC TCATAACTCT CTGTTGCCCA TGGAGGAAGA AAGTACATCT 
GATCATACAC ATGTTCAGCA GATCATGGAC AATGATGTAA ATGAAGCAAA TTTGATACCT 
GACAAAAGAG ACTTTCAGGT TCCAGCTGTG GACCTTGACT TTCGAGAATT GGAACACATA 
TTTCCACACT TGCATCGTCA GCTCTTTAAA CCCTTAGAAC CACATCTAGA TTTTGACTTA 
TCATCCCCTG GGACATCTCA AGAAGACAGC GACTTTTACC AGAGCTCAGA ATCTTCATCT 
GAAAAACATG TTAAGGCATT ATCCACAGGC ACAATCTGCT TCCCAGCACT GACAGCCAAG 
TCACATTCTC CTAACCCAAG GCTGAACCAA CTTGATATTA ACCTGGCTCA TGCAACAACT 
GAGGGATCTG AACAGTCTTT TCAACAGCTT CGACCAGAAT TCTCTTCACA GGAAAGCCAA 
CATGCTGACC TACCCAGTAT TTATAGCATT GAAGCAAGAG GTCCTTCCCA ACGCATGGAA 
AACCAGAACT ATTCTGAAAT GTTACAAAAT AAGAAAAAAA GTCTCTCTCT CCAGCCCTCA 
ACAGAGGATT TGACTCCAGC TTGCAGTTCA TCTGATACTG CTCTGTTTGA TCAGTTACAC 
CTGCAGCACA GCACTCCGTG TGCTTCTGTC TCTAGTGAGT GCTCAGTAAA ACTGTTAGAA 
AGCAGAGAAG AAGTGCTGGG ATTTGAAGAG CTGTCAAGAA GGGCAGTTAC AATGTCACAA 
AGACTCACTG AAGATGAAAA TGTAGTTCTA CCTATAAACC CACATGTAGG AAGAGTGGAG 
AAGGAGGCTT CAGTTCAGGG GTCTAATCCA TTGAGTATTC AAAATGAAAA GCCAATTCAG 
AACTTCATTG AAACTGACAC TACAGAAGCT GTAGGAAATG TATGTCAGCT AGCACAAGCA 
GAGCACATAT TAAAATCCTG TCCATTTCGG AGCCCTATTC CAATCTGGGA AACGGATACT 
GGCTATGGTA TAATGGAAGA ACCAGATCTC ACTCTAGTGA GTAATAGTGA CATCAGCATC 
ACTGAAACAG ATCTTGCAAA CTTAACCCTT GAAGACAGAG AAGACAATGA AGCACAGTTT 
TTTCAGGCAG GTGTCGTTCT GCCTACATCT TCAATGGAAA CCTCTGTTTG TGGAGCTGTA 
TCAGAGCCTT ATGTGGACCA ACCCACAGTG GCACCTTCAG CTACATCAGG TAGTTTACAA 
GAAGCATTTA TGACAAGACA GACACTTACA GAACGATCTT ACCAGAGGCA GAGAGAGATC 
TGGAATAAAA CTCGACTCCC TCAAACCAAA GTATCCAAAG AGAAGCTACC TACAGGTTGC 
ACAGGCAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240849.1
CDS	77..7468
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFVSRQMGTKQPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_011242547.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242547.1

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTGT GAGTAGACAG ATGGGTACAA AGCAGCCAGA TGCTCATTTG 
GCTGCTGAAG AAGAAGCTAG ACGAGTGGAA AGACTACGGA AACAGGCGGC ACAGGAGAGA 
GTGAAACAGT TTGAAAGGGC ACATGTGCGG GGATCCCAAG CCATGAAAAA GATCCACTTG 
GCCCAAAACC AGGAGCGATT GATGGAAGAA CTCAAACAGC TGCAGAAAGA GGACCTGGCC 
CGTAGGAGGC AGACCGTGGC ACAGATGCCG CCACAGATGC TGGAGTTGCC ATACCGACGG 
AGTGAGATGA AGGAAGACAG GCAGCGAGAG CTGGAGTTTG CCTTTGAAGA TATGTACAAT 
GCAGATAGGA AGGTGAAAGG AAACCTGATT TTACATCTTA AGCCAGAACC TTTGCCTACA 
ATCTCTGATC AGCTCCAAGA TGAAGAACTG GACCTTTCAA TGGAACAAGA AAATCAAGTG 
CCCTTGGCAG CAAAGATCCA ACAAATCCCC TCAAGAATTC TTTTTAAAAG ATTATTAAAC 
AAGATTAGAA GTCAAAAATC TCTCTGGACA ATTAAATCTG TGTCTGAGGA TGAAGGTGAG 
GTGACTTCGA GCATCATTGA GATTGAGAGT AAAGTGCCAA GCGTGGACTC GGGAGCCATT 
ATCACAGAGG AGAGAACAGC GGCGTCCTTT GAGCAGGAGC AAGTCACAGA CAGTGATAGG 
CTGACGATAG AATCTGGACC ACTGAGCAGT GAAGATAAGC CATTGTACTA CAAAGCGGGG 
ACTGGAAGGG AACAAGCAAT GGCAGTGTCT CCACCTGCCA CAGCTGTGGC TCAGAGTTCA 
GTCCTGCTTC ATCCTCAGGA AGAGGCAGTC AGAATTCGAA TGTCATTAAG GCGCAAACAG 
ATAATGGAGA TAGAAGAGCA GAAGCAGAAG CAGTTGGAAT TACTTGAACA AATTGAGCAA 
CAGAAGTTAA GGTTAGAAAC TGATTGTTTC CGGGCTCAAC TGGAAGAGCA AAGAAAGCAA 
GCTGATCAGC CTGAGGTTTG CTGTGCTCCA ATGTCACATG CTATGATTTC TGATGAAGAC 
TCTCACAGGC AGATGATTCG TAACTATCAA CATCAGCTTT TACAACAAAA CAGGTTACAC 
AAGGAGACTG TTGAAACTGC CAGGAAACGA TTACTCGAAT ACCAAACTGT ATTAAAAGAA 
AGATCCCCAT CCCTGTCAGC CTCAGCACTG GTACCTGATT CTGTTGTGTC AGGGCCACCA 
CAGCAATCCT ACAAGCCTGC TGCTGCTTCA GACTCTTGGG ATCCAAGCCA GAGACTGAAA 
CTAAGTCCTA GCAAATATCA ACCTGTGCAA CCCTCACAGA TCCCTGCATT AGAGCAGAGT 
CATATTCAGG TACCAAGACA TGGTCACATT ACACAGAGGC AAGGAAAAAT GGCTGTATCA 
GAAATGTTAG GTAAGCAGCC CGTGGAGTCA CAAGAACGTC AGTGGCAGTT CTCTCAGGTG 
GAAACACATC AGGGAGACTA TGAGTTCGTC CTTAAAGATT CTCATTCACT TTCAAGGACT 
TTGTCTTATG TTAGGCCACA AACATTACAA GATGCTAGAG AAGTATCTAA ACCTCCTAGG 
GTAATAATTT GTCAAAGTCT AGATTCCCAA CAGATATCAT CAGAGGATAG TGAAAATATA 
TCTTCTAAGC CAAGTGAACC GTCTCCTTTT CTCCCACTGG TGCCTGAACG CCCTTTTACT 
AGTCTCCCAG TTAAATTCCA TTCTGGAACA ATTCACAAAC CCTTTACGAC CATAAACCAG 
TCTGTAATCA GTCAAATGCA TGATCAACCT TTGTCATCCT CAGAGACTAT TACAGCTCAG 
CAGGGTGACT TAAGATTTCT TCAAGAACAG TTAGAACTAC AGAAGAAAGT TCTTCAGGCA 
AGACAAGAGG CTCGGGAAAA GCTGCTTTTG TGCACACAGA AAGAACTGGG ACAGCAGACT 
GGCCTCCCAG TGTTCCTCCC TTCACCAGCT GGAAATATAT TTTCTTCATT ACCCTCTGCC 
TCAGCTGAAT CAGGAAACTT TCAGACATCT TCAACAAAAA GTGATGCCAC TGTTTCCTCA 
GACAATATGG ACAGGCTTTG GGATTCTTCA CAACCCATCT CATCACAGCA AACTCATTTA 
GAATTTCTCC AAGAGCAGTC CAGTGTTGAG ACGGATAACC TTCAGGCTAG GCGGGAAGCC 
CAGGAGGTCT TGTTTGCACA TACACAAAAT ACGCTGGAAA AAATTGTACG TTCTGAACAA 
GCTGGGTCTT CTTTGCCACA TCAGGTAGCT CAGCAGTCAT TTAGTTCACT AACATTGGCT 
GATACACAAT CTAAAAAAAT CCAGAAACAG CCTTTACCTG CAAACAAGAA AGGGCTACTC 
CCAAGCCAGT CTGAAGTCTC AAAAGCTCAG GATGGATCTT CAGGTTTTCT ACAGCAGACC 
CTACCTCTCC AGAACACTTT AAAGTTGCTT CAAGAGCAGC TGACTAGACA GAGGAGTATG 
ATTCCGCCTA GACGTGATGG TCAAGAGACA TTACTTTTAT ATAAAGAAAG CTGTTCAGAA 
GACAGTGAGG CTGGGCCAGT AGAATCACTC TCCTCCGTGG TTGTTCAGCA TGCAGATGCT 
TCTCGTGCAG TTTCAGAAGT ACCTAAGCGG CTCCAGGACG TGTATTCATC TGAGGAAGAA 
AATAGAGTTC TCTCCAGTCA TTTGATCACC CATGGCTTTC CACAGCACAG CCTGCAAAGA 
CAAGAACATT TTACACCACT CCAGGAAGAG ACACACATTC AGAGGCTTAT ACTTGGTGCT 
AGAAAAAATA ATGAGGAATT TGCTCCCAAG CAAAATGAAT TAGAAAAGGG GCTCTGCTCT 
CAACAGACTG ATGCCTTGTC TTCTCCATCC CAAGTCACCG ACTGGGGAAC ATCCCGGGGG 
TCTGTATCAG TCAGAAGTGA TAGGACAGAC CCCTTAAGGC ATTTTAAGAT TCCAGCGTTT 
CGGGAAAGAC TAGTTAGAGT TTCACAACAT ACATTCCCTC TCCAAGATAA TTTGCAGGAA 
CACCAAGAAT GGGTAGACAC AGAAAAAGAG AGCTTTCAAT CTAGTCCTCT AACTCCAGAA 
AATCCATCTT CTCAACAGAC AGGTTTTTCG TCCTTCAAAG CCTCATTAAG ACTGCCATCG 
TGTGTGTCCT TGCCATCTGC TGACTCTGGT ATCACACAGC ACCCTCTCTC AACAGAGAGT 
GACAGTAAAG TCAAGTCAAG CCATCTTCAG ATACCAGAAC TGCAGCATAG ACTTTCGAAG 
ATATCCCAGC TCATCCCGCC TCAACAAGAC AGTTTGAAGG CCCTTCAAGA ACAATTAGCT 
ACACAAAGGG AAGCCATCAT CCACTCTAGA CAAGAAGCTC ATGAAGAAAC ACTAAGAGAA 
TGGAAGGAAA AAATATTCCC AGAGCAGGTT GGTCCATTTT CTCCCCTGAT ACCACAACAT 
TCACTTGCTT CATTCCCTGT TTCTGATACT GAAAGAGCAC AAGAACTGTG TTCAACCAAC 
AGTGACACCA TATCTTCAGG TTATCCTGAG ATGCTGGAGT TGCCTGACAG GACATTGGGT 
TTATCACATA CTGCTTTACC TCAGCAGAAT AATCTGACTG CACATCCAGA ACACCTTCAT 
GCACAAACAA ATTTTTTTCA CTCTACTGAG AAAGCCCAAG AAGGGTTGGT GTTTCCCAGA 
CCATGTCAAT TTGAAGAGAT GTCTGCTGAG CATTTTATCC AGCCTCAACA TGATGACTTA 
AAGGCACTTC AACAGCAGTT AGATATGCAA AGGGAAGCCA TTAGATCTGG CCAGGAAATG 
CAAGAGAAAA TGCTTTTGCA AAGGTTAAAT AAATTGGAGC AAAGGATATC TTCCAAACAA 
ATTAGCTCCT CTCTATTTTC ATCCCAGGTA GCACTGCCCA TTGCCAATTC TGACGGGACC 
TTACAGTCTT TCCCAACTAA AAGTAACGAG ACTGAGCTGC TTGGGTCCCA AGATGAGTAT 
CTGAGCTTTT CACAGCCTCG TCTGCCTCTG CAGAATAACA TGACAGAGCA GTTGGACTTA 
GAAAAGGTAT TTCATAAAGA ACTGCTTTTA CACAAACAAA AGAGCCAAAA CAAAAGTGAA 
TCTTCTGAGC ATTCCCTCCC TCCTTTGTTT CTGTCCAAGG AAATAGAGCA TCCATTCATT 
TCACTACCTT TTGCAGAAAG TAAATCTAAA AGCATTTGTG AGTTGTATTT ATCTGACAAG 
AAGCATGCAG CTCCTAATGA TGCTGTGATC CCAAGATTGC AGGACAGGCT TTTAAGCTGT 
TCCCAACCTG TCTTAACTCA GCAAGATAAC ATGAGTCTTC AGAAGCAGCT GAATCTACAA 
AGAGAAACTC TACACTCTAG GCAAAAAGCC CAAGAAGAAT TGCTTGTGCA GAGACAGACA 
TCCTTACAGC AGCAAATCCA AAGACACAGA GAGACTTTGA AAAATTTCTT TAATGTCAGT 
CAGGCAAGGA ATCCCACAGA TGAGAATGAC TTGGAAATGC AGAAGAGAGA ACAGCTCGGA 
GGGTGGTTTC CTCACACCCA AGGCCTTACC TGGGGAGACG CAGGTCAGGG GAGCGCTAAT 
GGTGAGCAGC CACGTGCAGA TGTCCATGCT GAGCACAATG GTGAGAGTCT GGCCAAAGAA 
CTGAGTGGTA GAGCCTCAAA GCCACCTGTC TCCAAAGTTA AATGTGTCTT GGACTTAAAC 
CAGCATGAGC TTAGTACTAT ACAGGAAGTA GAGTCACCAG CAAGTGGCAG GATTTCCATG 
CCAGGTAAAC AAGGAAAAGC AGAGTTTTAT CAAGACCGAG ACCCATTAAG GGTCTCAGTA 
AGCAGAGAAC AAAGTTTCTT GGAGAGCCCA CTGGCACATG ATCCATTTGG TTGTCACCAA 
CCACCTGCCC AAGAGAACAG CAAAAGCCAT GACGACAATG CTGAAGCAGT TAAAGTCAAG 
AAATCTGATG TTGAGGATCA TGCAGTATTG AGTCATGCTG TCTCAAAAGA AGAAGCGTGT 
ACAAACCTGG GTCCACTTGG AAAGCCAGAC GATGAGGCTG AAACACAAGA GATTTCTCAG 
GAGCCATTGT CTTCAGTAAC TGTTTCTACT GGAAGCTTCT TAAGTTATGA AATCACAGAT 
TTGAGCCTCA CAGATCCAGA GTCATTTTCA GAACAGACAG AGCATCTAGA ACAAGAATCT 
ACCAATAAGC AGGAAGAGAC TGATCCTCTT AGTATTGCAG TCCCTTCAGT TATTTATCAG 
CAACAGCATT CCTTAGGTGC TCATAACTCT CTGTTGCCCA TGGAGGAAGA AAGTACATCT 
GATCATACAC ATGTTCAGCA GATCATGGAC AATGATGTAA ATGAAGCAAA TTTGATACCT 
GACAAAAGAG ACTTTCAGGT TCCAGCTGTG GACCTTGACT TTCGAGAATT GGAACACATA 
TTTCCACACT TGCATCGTCA GCTCTTTAAA CCCTTAGAAC CACATCTAGA TTTTGACTTA 
TCATCCCCTG GGACATCTCA AGAAGACAGC GACTTTTACC AGAGCTCAGA ATCTTCATCT 
GAAAAACATG TTAAGGCATT ATCCACAGGC ACAATCTGCT TCCCAGCACT GACAGCCAAG 
TCACATTCTC CTAACCCAAG GCTGAACCAA CTTGATATTA ACCTGGCTCA TGCAACAACT 
GAGGGATCTG AACAGTCTTT TCAACAGCTT CGACCAGAAT TCTCTTCACA GGAAAGCCAA 
CATGCTGACC TACCCAGTAT TTATAGCATT GAAGCAAGAG GTCCTTCCCA ACGCATGGAA 
AACCAGAACT ATTCTGAAAT GTTACAAAAT AAGAAAAAAA GTCTCTCTCT CCAGCCCTCA 
ACAGAGGATT TGACTCCAGC TTGCAGTTCA TCTGATACTG CTCTGTTTGA TCAGTTACAC 
CTGCAGCACA GCACTCCGTG TGCTTCTGTC TCTAGTGAGT GCTCAGTAAA ACTGTTAGAA 
AGCAGAGAAG AAGTGCTGGG ATTTGAAGAG CTGTCAAGAA GGGCAGTTAC AATGTCACAA 
AGACTCACTG AAGATGAAAA TGTAGTTCTA CCTATAAACC CACATGTAGG AAGAGTGGAG 
AAGGAGGCTT CAGTTCAGGG GTCTAATCCA TTGAGTATTC AAAATGAAAA GCCAATTCAG 
AACTTCATTG AAACTGACAC TACAGAAGCT GTAGGAAATG TATGTCAGCT AGCACAAGCA 
GAGCACATAT TAAAATCCTG TCCATTTCGG AGCCCTATTC CAATCTGGGA AACGGATACT 
GGCTATGGTA TAATGGAAGA ACCAGATCTC ACTCTAGTGA GTAATAGTGA CATCAGCATC 
ACTGAAACAG ATCTTGCAAA CTTAACCCTT GAAGACAGAG AAGACAATGA AGCACAGTTT 
TTTCAGGCAG GTGTCGTTCT GCCTACATCT TCAATGGAAA CCTCTGTTTG TGGAGCTGTA 
TCAGAGCCTT ATGTGGACCA ACCCACAGTG GCACCTTCAG CTACATCAGG TAGTTTACAA 
GAAGCATTTA TGACAAGACA GACACTTACA GAACGATCTT ACCAGAGGCA GAGAGAGATC 
TGGAATAAAA CTCGACTCCC TCAAACCAAA GTATCCAAAG AGAAGCTACC TACAGGTTGC 
ACAGGCAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_011242548.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240850.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X5, mRNA.

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CloneID	OMu74559
Clone ID Related Accession (Same CDS sequence)	XM_011242548.2 , XM_011242548.3
Accession Version	XM_011242548.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242548.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTCC AGATGCTCAT TTGGCTGCTG AAGAAGAAGC TAGACGAGTG 
GAAAGACTAC GGAAACAGGC GGCACAGGAG AGAGTGAAAC AGTTTGAAAG GGCACATGTG 
CGGGGATCCC AAGCCATGAA AAAGATCCAC TTGGCCCAAA ACCAGGAGCG ATTGATGGAA 
GAACTCAAAC AGCTGCAGAA AGAGGACCTG GCCCGTAGGA GGCAGACCGT GGCACAGATG 
CCGCCACAGA TGCTGGAGTT GCCATACCGA CGGAGTGAGA TGAAGGAAGA CAGGCAGCGA 
GAGCTGGAGT TTGCCTTTGA AGATATGTAC AATGCAGATA GGAAGGTGAA AGGAAACCTG 
ATTTTACATC TTAAGCCAGA ACCTTTGCCT ACAATCTCTG ATCAGCTCCA AGATGAAGAA 
CTGGACCTTT CAATGGAACA AGAAAATCAA GTGCCCTTGG CAGCAAAGAT CCAACAAATC 
CCCTCAAGAA TTCTTTTTAA AAGATTATTA AACAAGATTA GAAGTCAAAA ATCTCTCTGG 
ACAATTAAAT CTGTGTCTGA GGATGAAGGT GAGGTGACTT CGAGCATCAT TGAGATTGAG 
AGTAAAGTGC CAAGCGTGGA CTCGGGAGCC ATTATCACAG AGGAGAGAAC AGCGGCGTCC 
TTTGAGCAGG AGCAAGTCAC AGACAGTGAT AGGCTGACGA TAGAATCTGG ACCACTGAGC 
AGTGAAGATA AGCCATTGTA CTACAAAGCG GGGACTGGAA GGGAACAAGC AATGGCAGTG 
TCTCCACCTG CCACAGCTGT GGCTCAGAGT TCAGTCCTGC TTCATCCTCA GGAAGAGGCA 
GTCAGAATTC GAATGTCATT AAGGCGCAAA CAGATAATGG AGATAGAAGA GCAGAAGCAG 
AAGCAGTTGG AATTACTTGA ACAAATTGAG CAACAGAAGT TAAGGTTAGA AACTGATTGT 
TTCCGGGCTC AACTGGAAGA GCAAAGAAAG CAAGCTGATC AGCCTGAGGT TTGCTGTGCT 
CCAATGTCAC ATGCTATGAT TTCTGATGAA GACTCTCACA GGCAGATGAT TCGTAACTAT 
CAACATCAGC TTTTACAACA AAACAGGTTA CACAAGGAGA CTGTTGAAAC TGCCAGGAAA 
CGATTACTCG AATACCAAAC TGTATTAAAA GAAAGATCCC CATCCCTGTC AGCCTCAGCA 
CTGGTACCTG ATTCTGTTGT GTCAGGGCCA CCACAGCAAT CCTACAAGCC TGCTGCTGCT 
TCAGACTCTT GGGATCCAAG CCAGAGACTG AAACTAAGTC CTAGCAAATA TCAACCTGTG 
CAACCCTCAC AGATCCCTGC ATTAGAGCAG AGTCATATTC AGGTACCAAG ACATGGTCAC 
ATTACACAGA GGCAAGGAAA AATGGCTGTA TCAGAAATGT TAGGTAAGCA GCCCGTGGAG 
TCACAAGAAC GTCAGTGGCA GTTCTCTCAG GTGGAAACAC ATCAGGGAGA CTATGAGTTC 
GTCCTTAAAG ATTCTCATTC ACTTTCAAGG ACTTTGTCTT ATGTTAGGCC ACAAACATTA 
CAAGATGCTA GAGAAGTATC TAAACCTCCT AGGGTAATAA TTTGTCAAAG TCTAGATTCC 
CAACAGATAT CATCAGAGGA TAGTGAAAAT ATATCTTCTA AGCCAAGTGA ACCGTCTCCT 
TTTCTCCCAC TGGTGCCTGA ACGCCCTTTT ACTAGTCTCC CAGTTAAATT CCATTCTGGA 
ACAATTCACA AACCCTTTAC GACCATAAAC CAGTCTGTAA TCAGTCAAAT GCATGATCAA 
CCTTTGTCAT CCTCAGAGAC TATTACAGCT CAGCAGGGTG ACTTAAGATT TCTTCAAGAA 
CAGTTAGAAC TACAGAAGAA AGTTCTTCAG GCAAGACAAG AGGCTCGGGA AAAGCTGCTT 
TTGTGCACAC AGAAAGAACT GGGACAGCAG ACTGGCCTCC CAGTGTTCCT CCCTTCACCA 
GCTGGAAATA TATTTTCTTC ATTACCCTCT GCCTCAGCTG AATCAGGAAA CTTTCAGACA 
TCTTCAACAA AAAGTGATGC CACTGTTTCC TCAGACAATA TGGACAGGCT TTGGGATTCT 
TCACAACCCA TCTCATCACA GCAAACTCAT TTAGAATTTC TCCAAGAGCA GTCCAGTGTT 
GAGACGGATA ACCTTCAGGC TAGGCGGGAA GCCCAGGAGG TCTTGTTTGC ACATACACAA 
AATACGCTGG AAAAAATTGT ACGTTCTGAA CAAGCTGGGT CTTCTTTGCC ACATCAGGTA 
GCTCAGCAGT CATTTAGTTC ACTAACATTG GCTGATACAC AATCTAAAAA AATCCAGAAA 
CAGCCTTTAC CTGCAAACAA GAAAGGGCTA CTCCCAAGCC AGTCTGAAGT CTCAAAAGCT 
CAGGATGGAT CTTCAGGTTT TCTACAGCAG ACCCTACCTC TCCAGAACAC TTTAAAGTTG 
CTTCAAGAGC AGCTGACTAG ACAGAGGAGT ATGATTCCGC CTAGACGTGA TGGTCAAGAG 
ACATTACTTT TATATAAAGA AAGCTGTTCA GAAGACAGTG AGGCTGGGCC AGTAGAATCA 
CTCTCCTCCG TGGTTGTTCA GCATGCAGAT GCTTCTCGTG CAGTTTCAGA AGTACCTAAG 
CGGCTCCAGG ACGTGTATTC ATCTGAGGAA GAAAATAGAG TTCTCTCCAG TCATTTGATC 
ACCCATGGCT TTCCACAGCA CAGCCTGCAA AGACAAGAAC ATTTTACACC ACTCCAGGAA 
GAGACACACA TTCAGAGGCT TATACTTGGT GCTAGAAAAA ATAATGAGGA ATTTGCTCCC 
AAGCAAAATG AATTAGAAAA GGGGCTCTGC TCTCAACAGA CTGATGCCTT GTCTTCTCCA 
TCCCAAGTCA CCGACTGGGG AACATCCCGG GGGTCTGTAT CAGTCAGAAG TGATAGGACA 
GACCCCTTAA GGCATTTTAA GATTCCAGCG TTTCGGGAAA GACTAGTTAG AGTTTCACAA 
CATACATTCC CTCTCCAAGA TAATTTGCAG GAACACCAAG AATGGGTAGA CACAGAAAAA 
GAGAGCTTTC AATCTAGTCC TCTAACTCCA GAAAATCCAT CTTCTCAACA GACAGGTTTT 
TCGTCCTTCA AAGCCTCATT AAGACTGCCA TCGTGTGTGT CCTTGCCATC TGCTGACTCT 
GGTATCACAC AGCACCCTCT CTCAACAGAG AGTGACAGTA AAGTCAAGTC AAGCCATCTT 
CAGATACCAG AACTGCAGCA TAGACTTTCG AAGATATCCC AGCTCATCCC GCCTCAACAA 
GACAGTTTGA AGGCCCTTCA AGAACAATTA GCTACACAAA GGGAAGCCAT CATCCACTCT 
AGACAAGAAG CTCATGAAGA AACACTAAGA GAATGGAAGG AAAAAATATT CCCAGAGCAG 
GTTGGTCCAT TTTCTCCCCT GATACCACAA CATTCACTTG CTTCATTCCC TGTTTCTGAT 
ACTGAAAGAG CACAAGAACT GTGTTCAACC AACAGTGACA CCATATCTTC AGGTTATCCT 
GAGATGCTGG AGTTGCCTGA CAGGACATTG GGTTTATCAC ATACTGCTTT ACCTCAGCAG 
AATAATCTGA CTGCACATCC AGAACACCTT CATGCACAAA CAAATTTTTT TCACTCTACT 
GAGAAAGCCC AAGAAGGGTT GGTGTTTCCC AGACCATGTC AATTTGAAGA GATGTCTGCT 
GAGCATTTTA TCCAGCCTCA ACATGATGAC TTAAAGGCAC TTCAACAGCA GTTAGATATG 
CAAAGGGAAG CCATTAGATC TGGCCAGGAA ATGCAAGAGA AAATGCTTTT GCAAAGGTTA 
AATAAATTGG AGCAAAGGAT ATCTTCCAAA CAAATTAGCT CCTCTCTATT TTCATCCCAG 
GTAGCACTGC CCATTGCCAA TTCTGACGGG ACCTTACAGT CTTTCCCAAC TAAAAGTAAC 
GAGACTGAGC TGCTTGGGTC CCAAGATGAG TATCTGAGCT TTTCACAGCC TCGTCTGCCT 
CTGCAGAATA ACATGACAGA GCAGTTGGAC TTAGAAAAGG TATTTCATAA AGAACTGCTT 
TTACACAAAC AAAAGAGCCA AAACAAAAGT GAATCTTCTG AGCATTCCCT CCCTCCTTTG 
TTTCTGTCCA AGGAAATAGA GCATCCATTC ATTTCACTAC CTTTTGCAGA AAGTAAATCT 
AAAAGCATTT GTGAGTTGTA TTTATCTGAC AAGAAGCATG CAGCTCCTAA TGATGCTGTG 
ATCCCAAGAT TGCAGGACAG GCTTTTAAGC TGTTCCCAAC CTGTCTTAAC TCAGCAAGAT 
AACATGAGTC TTCAGAAGCA GCTGAATCTA CAAAGAGAAA CTCTACACTC TAGGCAAAAA 
GCCCAAGAAG AATTGCTTGT GCAGAGACAG ACATCCTTAC AGCAGCAAAT CCAAAGACAC 
AGAGAGACTT TGAAAAATTT CTTTAATGTC AGTCAGGCAA GGAATCCCAC AGATGAGAAT 
GACTTGGAAA TGCAGAAGAG AGAACAGCTC GGAGGGTGGT TTCCTCACAC CCAAGGCCTT 
ACCTGGGGAG ACGCAGGTCA GGGGAGCGCT AATGGTGAGC AGCCACGTGC AGATGTCCAT 
GCTGAGCACA ATGGTGAGAG TCTGGCCAAA GAACTGAGTG GTAGAGCCTC AAAGCCACCT 
GTCTCCAAAG TTAAATGTGT CTTGGACTTA AACCAGCATG AGCTTAGTAC TATACAGGAA 
GTAGAGTCAC CAGCAAGTGG CAGGATTTCC ATGCCAGGTA AACAAGGAAA AGCAGAGTTT 
TATCAAGACC GAGACCCATT AAGGGTCTCA GTAAGCAGAG AACAAAGTTT CTTGGAGAGC 
CCACTGGCAC ATGATCCATT TGGTTGTCAC CAACCACCTG CCCAAGAGAA CAGCAAAAGC 
CATGACGACA ATGCTGAAGC AGTTAAAGTC AAGAAATCTG ATGTTGAGGA TCATGCAGTA 
TTGAGTCATG CTGTCTCAAA AGAAGAAGCG TGTACAAACC TGGGTCCACT TGGAAAGCCA 
GACGATGAGG CTGAAACACA AGAGATTTCT CAGGAGCCAT TGTCTTCAGT AACTGTTTCT 
ACTGGAAGCT TCTTAAGTTA TGAAATCACA GATTTGAGCC TCACAGATCC AGAGTCATTT 
TCAGAACAGA CAGAGCATCT AGAACAAGAA TCTACCAATA AGCAGGAAGA GACTGATCCT 
CTTAGTATTG CAGTCCCTTC AGTTATTTAT CAGCAACAGC ATTCCTTAGG TGCTCATAAC 
TCTCTGTTGC CCATGGAGGA AGAAAGTACA TCTGATCATA CACATGTTCA GCAGATCATG 
GACAATGATG TAAATGAAGC AAATTTGATA CCTGACAAAA GAGACTTTCA GGTTCCAGCT 
GTGGACCTTG ACTTTCGAGA ATTGGAACAC ATATTTCCAC ACTTGCATCG TCAGCTCTTT 
AAACCCTTAG AACCACATCT AGATTTTGAC TTATCATCCC CTGGGACATC TCAAGAAGAC 
AGCGACTTTT ACCAGCAGAG CTCAGAATCT TCATCTGAAA AACATGTTAA GGCATTATCC 
ACAGGCACAA TCTGCTTCCC AGCACTGACA GCCAAGTCAC ATTCTCCTAA CCCAAGGCTG 
AACCAACTTG ATATTAACCT GGCTCATGCA ACAACTGAGG GATCTGAACA GTCTTTTCAA 
CAGCTTCGAC CAGAATTCTC TTCACAGGAA AGCCAACATG CTGACCTACC CAGTATTTAT 
AGCATTGAAG CAAGAGGTCC TTCCCAACGC ATGGAAAACC AGAACTATTC TGAAATGTTA 
CAAAATAAGA AAAAAAGTCT CTCTCTCCAG CCCTCAACAG AGGATTTGAC TCCAGCTTGC 
AGTTCATCTG ATACTGCTCT GTTTGATCAG TTACACCTGC AGCACAGCAC TCCGTGTGCT 
TCTGTCTCTA GTGAGTGCTC AGTAAAACTG TTAGAAAGCA GAGAAGAAGT GCTGGGATTT 
GAAGAGCTGT CAAGAAGGGC AGTTACAATG TCACAAAGAC TCACTGAAGA TGAAAATGTA 
GTTCTACCTA TAAACCCACA TGTAGGAAGA GTGGAGAAGG AGGCTTCAGT TCAGGGGTCT 
AATCCATTGA GTATTCAAAA TGAAAAGCCA ATTCAGAACT TCATTGAAAC TGACACTACA 
GAAGCTGTAG GAAATGTATG TCAGCTAGCA CAAGCAGAGC ACATATTAAA ATCCTGTCCA 
TTTCGGAGCC CTATTCCAAT CTGGGAAACG GATACTGGCT ATGGTATAAT GGAAGAACCA 
GATCTCACTC TAGTGAGTAA TAGTGACATC AGCATCACTG AAACAGATCT TGCAAACTTA 
ACCCTTGAAG ACAGAGAAGA CAATGAAGCA CAGTTTTTTC AGGCAGGTGT CGTTCTGCCT 
ACATCTTCAA TGGAAACCTC TGTTTGTGGA GCTGTATCAG AGCCTTATGT GGACCAACCC 
ACAGTGGCAC CTTCAGCTAC ATCAGGTAGT TTACAAGAAG CATTTATGAC AAGACAGACA 
CTTACAGAAC GATCTTACCA GAGGCAGAGA GAGATCTGGA ATAAAACTCG ACTCCCTCAA 
ACCAAAGTAT CCAAAGAGAA GCTACCTACA GGTTGCACAG GCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240850.1
CDS	78..7445
Translation	MTEVTRAGSWLSRRYPNGCWVAGRVRVPVCREARPHTLSDPLYLTYSEMKRKGMNTKLRLSPSEEAFILKEDYERRRKLRLLQVREQERGIAFQIREGIKQRRSQQVSHLAEELRAKWEEAQSQKIQNLEKLYLASLRHMGDGHRQAKENEPDLDALSRRAAERKRKAEVRHKEALKVQKNQKEMLMKQKTRHIKARKEAVLVEKQRSAKMARLPPPVPSPFENIDVNRIPSLKTNSSTYHHISTFPDAHLAAEEEARRVERLRKQAAQERVKQFERAHVRGSQAMKKIHLAQNQERLMEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_011242548.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242548.2

ORF Insert Sequence:

ATGACGGAAG TGACGAGAGC CGGGTCATGG CTGTCCCGCA GGTACCCAAA CGGCTGCTGG 
GTAGCCGGCC GGGTGCGGGT GCCTGTGTGT CGTGAGGCCA GACCACACAC GCTGTCAGAT 
CCTTTATATT TAACATACTC AGAAATGAAA AGAAAGGGAA TGAATACAAA GCTGAGATTG 
AGCCCTAGTG AAGAAGCCTT CATTTTGAAG GAAGATTATG AAAGAAGACG AAAACTAAGA 
TTGCTGCAGG TTCGAGAACA AGAAAGGGGC ATTGCCTTCC AGATAAGAGA AGGCATAAAA 
CAGAGGCGAA GTCAGCAGGT TTCTCATTTG GCTGAGGAGC TAAGGGCAAA ATGGGAAGAA 
GCACAAAGCC AGAAAATACA GAACTTGGAA AAACTATATT TGGCAAGTTT AAGACATATG 
GGAGATGGAC ATCGACAGGC TAAAGAAAAT GAACCTGACT TGGATGCTCT TTCTCGGCGG 
GCAGCAGAAA GAAAAAGAAA AGCAGAAGTG AGGCATAAAG AAGCCCTAAA AGTGCAGAAA 
AATCAAAAAG AAATGTTAAT GAAACAGAAA ACCAGGCACA TAAAAGCTCG AAAGGAAGCA 
GTGCTTGTGG AAAAACAGAG ATCTGCTAAA ATGGCCAGGC TGCCACCGCC TGTCCCATCC 
CCCTTTGAGA ACATTGACGT GAACAGAATT CCTTCATTGA AAACTAACAG TTCCACTTAC 
CATCATATTT CCACCTTTCC AGATGCTCAT TTGGCTGCTG AAGAAGAAGC TAGACGAGTG 
GAAAGACTAC GGAAACAGGC GGCACAGGAG AGAGTGAAAC AGTTTGAAAG GGCACATGTG 
CGGGGATCCC AAGCCATGAA AAAGATCCAC TTGGCCCAAA ACCAGGAGCG ATTGATGGAA 
GAACTCAAAC AGCTGCAGAA AGAGGACCTG GCCCGTAGGA GGCAGACCGT GGCACAGATG 
CCGCCACAGA TGCTGGAGTT GCCATACCGA CGGAGTGAGA TGAAGGAAGA CAGGCAGCGA 
GAGCTGGAGT TTGCCTTTGA AGATATGTAC AATGCAGATA GGAAGGTGAA AGGAAACCTG 
ATTTTACATC TTAAGCCAGA ACCTTTGCCT ACAATCTCTG ATCAGCTCCA AGATGAAGAA 
CTGGACCTTT CAATGGAACA AGAAAATCAA GTGCCCTTGG CAGCAAAGAT CCAACAAATC 
CCCTCAAGAA TTCTTTTTAA AAGATTATTA AACAAGATTA GAAGTCAAAA ATCTCTCTGG 
ACAATTAAAT CTGTGTCTGA GGATGAAGGT GAGGTGACTT CGAGCATCAT TGAGATTGAG 
AGTAAAGTGC CAAGCGTGGA CTCGGGAGCC ATTATCACAG AGGAGAGAAC AGCGGCGTCC 
TTTGAGCAGG AGCAAGTCAC AGACAGTGAT AGGCTGACGA TAGAATCTGG ACCACTGAGC 
AGTGAAGATA AGCCATTGTA CTACAAAGCG GGGACTGGAA GGGAACAAGC AATGGCAGTG 
TCTCCACCTG CCACAGCTGT GGCTCAGAGT TCAGTCCTGC TTCATCCTCA GGAAGAGGCA 
GTCAGAATTC GAATGTCATT AAGGCGCAAA CAGATAATGG AGATAGAAGA GCAGAAGCAG 
AAGCAGTTGG AATTACTTGA ACAAATTGAG CAACAGAAGT TAAGGTTAGA AACTGATTGT 
TTCCGGGCTC AACTGGAAGA GCAAAGAAAG CAAGCTGATC AGCCTGAGGT TTGCTGTGCT 
CCAATGTCAC ATGCTATGAT TTCTGATGAA GACTCTCACA GGCAGATGAT TCGTAACTAT 
CAACATCAGC TTTTACAACA AAACAGGTTA CACAAGGAGA CTGTTGAAAC TGCCAGGAAA 
CGATTACTCG AATACCAAAC TGTATTAAAA GAAAGATCCC CATCCCTGTC AGCCTCAGCA 
CTGGTACCTG ATTCTGTTGT GTCAGGGCCA CCACAGCAAT CCTACAAGCC TGCTGCTGCT 
TCAGACTCTT GGGATCCAAG CCAGAGACTG AAACTAAGTC CTAGCAAATA TCAACCTGTG 
CAACCCTCAC AGATCCCTGC ATTAGAGCAG AGTCATATTC AGGTACCAAG ACATGGTCAC 
ATTACACAGA GGCAAGGAAA AATGGCTGTA TCAGAAATGT TAGGTAAGCA GCCCGTGGAG 
TCACAAGAAC GTCAGTGGCA GTTCTCTCAG GTGGAAACAC ATCAGGGAGA CTATGAGTTC 
GTCCTTAAAG ATTCTCATTC ACTTTCAAGG ACTTTGTCTT ATGTTAGGCC ACAAACATTA 
CAAGATGCTA GAGAAGTATC TAAACCTCCT AGGGTAATAA TTTGTCAAAG TCTAGATTCC 
CAACAGATAT CATCAGAGGA TAGTGAAAAT ATATCTTCTA AGCCAAGTGA ACCGTCTCCT 
TTTCTCCCAC TGGTGCCTGA ACGCCCTTTT ACTAGTCTCC CAGTTAAATT CCATTCTGGA 
ACAATTCACA AACCCTTTAC GACCATAAAC CAGTCTGTAA TCAGTCAAAT GCATGATCAA 
CCTTTGTCAT CCTCAGAGAC TATTACAGCT CAGCAGGGTG ACTTAAGATT TCTTCAAGAA 
CAGTTAGAAC TACAGAAGAA AGTTCTTCAG GCAAGACAAG AGGCTCGGGA AAAGCTGCTT 
TTGTGCACAC AGAAAGAACT GGGACAGCAG ACTGGCCTCC CAGTGTTCCT CCCTTCACCA 
GCTGGAAATA TATTTTCTTC ATTACCCTCT GCCTCAGCTG AATCAGGAAA CTTTCAGACA 
TCTTCAACAA AAAGTGATGC CACTGTTTCC TCAGACAATA TGGACAGGCT TTGGGATTCT 
TCACAACCCA TCTCATCACA GCAAACTCAT TTAGAATTTC TCCAAGAGCA GTCCAGTGTT 
GAGACGGATA ACCTTCAGGC TAGGCGGGAA GCCCAGGAGG TCTTGTTTGC ACATACACAA 
AATACGCTGG AAAAAATTGT ACGTTCTGAA CAAGCTGGGT CTTCTTTGCC ACATCAGGTA 
GCTCAGCAGT CATTTAGTTC ACTAACATTG GCTGATACAC AATCTAAAAA AATCCAGAAA 
CAGCCTTTAC CTGCAAACAA GAAAGGGCTA CTCCCAAGCC AGTCTGAAGT CTCAAAAGCT 
CAGGATGGAT CTTCAGGTTT TCTACAGCAG ACCCTACCTC TCCAGAACAC TTTAAAGTTG 
CTTCAAGAGC AGCTGACTAG ACAGAGGAGT ATGATTCCGC CTAGACGTGA TGGTCAAGAG 
ACATTACTTT TATATAAAGA AAGCTGTTCA GAAGACAGTG AGGCTGGGCC AGTAGAATCA 
CTCTCCTCCG TGGTTGTTCA GCATGCAGAT GCTTCTCGTG CAGTTTCAGA AGTACCTAAG 
CGGCTCCAGG ACGTGTATTC ATCTGAGGAA GAAAATAGAG TTCTCTCCAG TCATTTGATC 
ACCCATGGCT TTCCACAGCA CAGCCTGCAA AGACAAGAAC ATTTTACACC ACTCCAGGAA 
GAGACACACA TTCAGAGGCT TATACTTGGT GCTAGAAAAA ATAATGAGGA ATTTGCTCCC 
AAGCAAAATG AATTAGAAAA GGGGCTCTGC TCTCAACAGA CTGATGCCTT GTCTTCTCCA 
TCCCAAGTCA CCGACTGGGG AACATCCCGG GGGTCTGTAT CAGTCAGAAG TGATAGGACA 
GACCCCTTAA GGCATTTTAA GATTCCAGCG TTTCGGGAAA GACTAGTTAG AGTTTCACAA 
CATACATTCC CTCTCCAAGA TAATTTGCAG GAACACCAAG AATGGGTAGA CACAGAAAAA 
GAGAGCTTTC AATCTAGTCC TCTAACTCCA GAAAATCCAT CTTCTCAACA GACAGGTTTT 
TCGTCCTTCA AAGCCTCATT AAGACTGCCA TCGTGTGTGT CCTTGCCATC TGCTGACTCT 
GGTATCACAC AGCACCCTCT CTCAACAGAG AGTGACAGTA AAGTCAAGTC AAGCCATCTT 
CAGATACCAG AACTGCAGCA TAGACTTTCG AAGATATCCC AGCTCATCCC GCCTCAACAA 
GACAGTTTGA AGGCCCTTCA AGAACAATTA GCTACACAAA GGGAAGCCAT CATCCACTCT 
AGACAAGAAG CTCATGAAGA AACACTAAGA GAATGGAAGG AAAAAATATT CCCAGAGCAG 
GTTGGTCCAT TTTCTCCCCT GATACCACAA CATTCACTTG CTTCATTCCC TGTTTCTGAT 
ACTGAAAGAG CACAAGAACT GTGTTCAACC AACAGTGACA CCATATCTTC AGGTTATCCT 
GAGATGCTGG AGTTGCCTGA CAGGACATTG GGTTTATCAC ATACTGCTTT ACCTCAGCAG 
AATAATCTGA CTGCACATCC AGAACACCTT CATGCACAAA CAAATTTTTT TCACTCTACT 
GAGAAAGCCC AAGAAGGGTT GGTGTTTCCC AGACCATGTC AATTTGAAGA GATGTCTGCT 
GAGCATTTTA TCCAGCCTCA ACATGATGAC TTAAAGGCAC TTCAACAGCA GTTAGATATG 
CAAAGGGAAG CCATTAGATC TGGCCAGGAA ATGCAAGAGA AAATGCTTTT GCAAAGGTTA 
AATAAATTGG AGCAAAGGAT ATCTTCCAAA CAAATTAGCT CCTCTCTATT TTCATCCCAG 
GTAGCACTGC CCATTGCCAA TTCTGACGGG ACCTTACAGT CTTTCCCAAC TAAAAGTAAC 
GAGACTGAGC TGCTTGGGTC CCAAGATGAG TATCTGAGCT TTTCACAGCC TCGTCTGCCT 
CTGCAGAATA ACATGACAGA GCAGTTGGAC TTAGAAAAGG TATTTCATAA AGAACTGCTT 
TTACACAAAC AAAAGAGCCA AAACAAAAGT GAATCTTCTG AGCATTCCCT CCCTCCTTTG 
TTTCTGTCCA AGGAAATAGA GCATCCATTC ATTTCACTAC CTTTTGCAGA AAGTAAATCT 
AAAAGCATTT GTGAGTTGTA TTTATCTGAC AAGAAGCATG CAGCTCCTAA TGATGCTGTG 
ATCCCAAGAT TGCAGGACAG GCTTTTAAGC TGTTCCCAAC CTGTCTTAAC TCAGCAAGAT 
AACATGAGTC TTCAGAAGCA GCTGAATCTA CAAAGAGAAA CTCTACACTC TAGGCAAAAA 
GCCCAAGAAG AATTGCTTGT GCAGAGACAG ACATCCTTAC AGCAGCAAAT CCAAAGACAC 
AGAGAGACTT TGAAAAATTT CTTTAATGTC AGTCAGGCAA GGAATCCCAC AGATGAGAAT 
GACTTGGAAA TGCAGAAGAG AGAACAGCTC GGAGGGTGGT TTCCTCACAC CCAAGGCCTT 
ACCTGGGGAG ACGCAGGTCA GGGGAGCGCT AATGGTGAGC AGCCACGTGC AGATGTCCAT 
GCTGAGCACA ATGGTGAGAG TCTGGCCAAA GAACTGAGTG GTAGAGCCTC AAAGCCACCT 
GTCTCCAAAG TTAAATGTGT CTTGGACTTA AACCAGCATG AGCTTAGTAC TATACAGGAA 
GTAGAGTCAC CAGCAAGTGG CAGGATTTCC ATGCCAGGTA AACAAGGAAA AGCAGAGTTT 
TATCAAGACC GAGACCCATT AAGGGTCTCA GTAAGCAGAG AACAAAGTTT CTTGGAGAGC 
CCACTGGCAC ATGATCCATT TGGTTGTCAC CAACCACCTG CCCAAGAGAA CAGCAAAAGC 
CATGACGACA ATGCTGAAGC AGTTAAAGTC AAGAAATCTG ATGTTGAGGA TCATGCAGTA 
TTGAGTCATG CTGTCTCAAA AGAAGAAGCG TGTACAAACC TGGGTCCACT TGGAAAGCCA 
GACGATGAGG CTGAAACACA AGAGATTTCT CAGGAGCCAT TGTCTTCAGT AACTGTTTCT 
ACTGGAAGCT TCTTAAGTTA TGAAATCACA GATTTGAGCC TCACAGATCC AGAGTCATTT 
TCAGAACAGA CAGAGCATCT AGAACAAGAA TCTACCAATA AGCAGGAAGA GACTGATCCT 
CTTAGTATTG CAGTCCCTTC AGTTATTTAT CAGCAACAGC ATTCCTTAGG TGCTCATAAC 
TCTCTGTTGC CCATGGAGGA AGAAAGTACA TCTGATCATA CACATGTTCA GCAGATCATG 
GACAATGATG TAAATGAAGC AAATTTGATA CCTGACAAAA GAGACTTTCA GGTTCCAGCT 
GTGGACCTTG ACTTTCGAGA ATTGGAACAC ATATTTCCAC ACTTGCATCG TCAGCTCTTT 
AAACCCTTAG AACCACATCT AGATTTTGAC TTATCATCCC CTGGGACATC TCAAGAAGAC 
AGCGACTTTT ACCAGCAGAG CTCAGAATCT TCATCTGAAA AACATGTTAA GGCATTATCC 
ACAGGCACAA TCTGCTTCCC AGCACTGACA GCCAAGTCAC ATTCTCCTAA CCCAAGGCTG 
AACCAACTTG ATATTAACCT GGCTCATGCA ACAACTGAGG GATCTGAACA GTCTTTTCAA 
CAGCTTCGAC CAGAATTCTC TTCACAGGAA AGCCAACATG CTGACCTACC CAGTATTTAT 
AGCATTGAAG CAAGAGGTCC TTCCCAACGC ATGGAAAACC AGAACTATTC TGAAATGTTA 
CAAAATAAGA AAAAAAGTCT CTCTCTCCAG CCCTCAACAG AGGATTTGAC TCCAGCTTGC 
AGTTCATCTG ATACTGCTCT GTTTGATCAG TTACACCTGC AGCACAGCAC TCCGTGTGCT 
TCTGTCTCTA GTGAGTGCTC AGTAAAACTG TTAGAAAGCA GAGAAGAAGT GCTGGGATTT 
GAAGAGCTGT CAAGAAGGGC AGTTACAATG TCACAAAGAC TCACTGAAGA TGAAAATGTA 
GTTCTACCTA TAAACCCACA TGTAGGAAGA GTGGAGAAGG AGGCTTCAGT TCAGGGGTCT 
AATCCATTGA GTATTCAAAA TGAAAAGCCA ATTCAGAACT TCATTGAAAC TGACACTACA 
GAAGCTGTAG GAAATGTATG TCAGCTAGCA CAAGCAGAGC ACATATTAAA ATCCTGTCCA 
TTTCGGAGCC CTATTCCAAT CTGGGAAACG GATACTGGCT ATGGTATAAT GGAAGAACCA 
GATCTCACTC TAGTGAGTAA TAGTGACATC AGCATCACTG AAACAGATCT TGCAAACTTA 
ACCCTTGAAG ACAGAGAAGA CAATGAAGCA CAGTTTTTTC AGGCAGGTGT CGTTCTGCCT 
ACATCTTCAA TGGAAACCTC TGTTTGTGGA GCTGTATCAG AGCCTTATGT GGACCAACCC 
ACAGTGGCAC CTTCAGCTAC ATCAGGTAGT TTACAAGAAG CATTTATGAC AAGACAGACA 
CTTACAGAAC GATCTTACCA GAGGCAGAGA GAGATCTGGA ATAAAACTCG ACTCCCTCAA 
ACCAAAGTAT CCAAAGAGAA GCTACCTACA GGTTGCACAG GCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep295 ( XM_011242550.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240852.1 Mus musculus centrosomal protein 295 (Cep295), transcript variant X9, mRNA.

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CloneID	OMu74561
Clone ID Related Accession (Same CDS sequence)	XM_011242550.2 , XM_011242550.3
Accession Version	XM_011242550.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosomal protein of 295 kDa isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242550.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAC TCAAACAGCT GCAGAAAGAG GACCTGGCCC GTAGGAGGCA GACCGTGGCA 
CAGATGCCGC CACAGATGCT GGAGTTGCCA TACCGACGGA GTGAGATGAA GGAAGACAGG 
CAGCGAGAGC TGGAGTTTGC CTTTGAAGAT ATGTACAATG CAGATAGGAA GGTGAAAGGA 
AACCTGATTT TACATCTTAA GCCAGAACCT TTGCCTACAA TCTCTGATCA GCTCCAAGAT 
GAAGAACTGG ACCTTTCAAT GGAACAAGAA AATCAAGTGC CCTTGGCAGC AAAGATCCAA 
CAAATCCCCT CAAGAATTCT TTTTAAAAGA TTATTAAACA AGATTAGAAG TCAAAAATCT 
CTCTGGACAA TTAAATCTGT GTCTGAGGAT GAAGGTGAGG TGACTTCGAG CATCATTGAG 
ATTGAGAGTA AAGTGCCAAG CGTGGACTCG GGAGCCATTA TCACAGAGGA GAGAACAGCG 
GCGTCCTTTG AGCAGGAGCA AGTCACAGAC AGTGATAGGC TGACGATAGA ATCTGGACCA 
CTGAGCAGTG AAGATAAGCC ATTGTACTAC AAAGCGGGGA CTGGAAGGGA ACAAGCAATG 
GCAGTGTCTC CACCTGCCAC AGCTGTGGCT CAGAGTTCAG TCCTGCTTCA TCCTCAGGAA 
GAGGCAGTCA GAATTCGAAT GTCATTAAGG CGCAAACAGA TAATGGAGAT AGAAGAGCAG 
AAGCAGAAGC AGTTGGAATT ACTTGAACAA ATTGAGCAAC AGAAGTTAAG GTTAGAAACT 
GATTGTTTCC GGGCTCAACT GGAAGAGCAA AGAAAGCAAG CTGATCAGCC TGAGGTTTGC 
TGTGCTCCAA TGTCACATGC TATGATTTCT GATGAAGACT CTCACAGGCA GATGATTCGT 
AACTATCAAC ATCAGCTTTT ACAACAAAAC AGGTTACACA AGGAGACTGT TGAAACTGCC 
AGGAAACGAT TACTCGAATA CCAAACTGTA TTAAAAGAAA GATCCCCATC CCTGTCAGCC 
TCAGCACTGG TACCTGATTC TGTTGTGTCA GGGCCACCAC AGCAATCCTA CAAGCCTGCT 
GCTGCTTCAG ACTCTTGGGA TCCAAGCCAG AGACTGAAAC TAAGTCCTAG CAAATATCAA 
CCTGTGCAAC CCTCACAGAT CCCTGCATTA GAGCAGAGTC ATATTCAGGT ACCAAGACAT 
GGTCACATTA CACAGAGGCA AGGAAAAATG GCTGTATCAG AAATGTTAGG TAAGCAGCCC 
GTGGAGTCAC AAGAACGTCA GTGGCAGTTC TCTCAGGTGG AAACACATCA GGGAGACTAT 
GAGTTCGTCC TTAAAGATTC TCATTCACTT TCAAGGACTT TGTCTTATGT TAGGCCACAA 
ACATTACAAG ATGCTAGAGA AGTATCTAAA CCTCCTAGGG TAATAATTTG TCAAAGTCTA 
GATTCCCAAC AGATATCATC AGAGGATAGT GAAAATATAT CTTCTAAGCC AAGTGAACCG 
TCTCCTTTTC TCCCACTGGT GCCTGAACGC CCTTTTACTA GTCTCCCAGT TAAATTCCAT 
TCTGGAACAA TTCACAAACC CTTTACGACC ATAAACCAGT CTGTAATCAG TCAAATGCAT 
GATCAACCTT TGTCATCCTC AGAGACTATT ACAGCTCAGC AGGGTGACTT AAGATTTCTT 
CAAGAACAGT TAGAACTACA GAAGAAAGTT CTTCAGGCAA GACAAGAGGC TCGGGAAAAG 
CTGCTTTTGT GCACACAGAA AGAACTGGGA CAGCAGACTG GCCTCCCAGT GTTCCTCCCT 
TCACCAGCTG GAAATATATT TTCTTCATTA CCCTCTGCCT CAGCTGAATC AGGAAACTTT 
CAGACATCTT CAACAAAAAG TGATGCCACT GTTTCCTCAG ACAATATGGA CAGGCTTTGG 
GATTCTTCAC AACCCATCTC ATCACAGCAA ACTCATTTAG AATTTCTCCA AGAGCAGTCC 
AGTGTTGAGA CGGATAACCT TCAGGCTAGG CGGGAAGCCC AGGAGGTCTT GTTTGCACAT 
ACACAAAATA CGCTGGAAAA AATTGTACGT TCTGAACAAG CTGGGTCTTC TTTGCCACAT 
CAGGTAGCTC AGCAGTCATT TAGTTCACTA ACATTGGCTG ATACACAATC TAAAAAAATC 
CAGAAACAGC CTTTACCTGC AAACAAGAAA GGGCTACTCC CAAGCCAGTC TGAAGTCTCA 
AAAGCTCAGG ATGGATCTTC AGGTTTTCTA CAGCAGACCC TACCTCTCCA GAACACTTTA 
AAGTTGCTTC AAGAGCAGCT GACTAGACAG AGGAGTATGA TTCCGCCTAG ACGTGATGGT 
CAAGAGACAT TACTTTTATA TAAAGAAAGC TGTTCAGAAG ACAGTGAGGC TGGGCCAGTA 
GAATCACTCT CCTCCGTGGT TGTTCAGCAT GCAGATGCTT CTCGTGCAGT TTCAGAAGTA 
CCTAAGCGGC TCCAGGACGT GTATTCATCT GAGGAAGAAA ATAGAGTTCT CTCCAGTCAT 
TTGATCACCC ATGGCTTTCC ACAGCACAGC CTGCAAAGAC AAGAACATTT TACACCACTC 
CAGGAAGAGA CACACATTCA GAGGCTTATA CTTGGTGCTA GAAAAAATAA TGAGGAATTT 
GCTCCCAAGC AAAATGAATT AGAAAAGGGG CTCTGCTCTC AACAGACTGA TGCCTTGTCT 
TCTCCATCCC AAGTCACCGA CTGGGGAACA TCCCGGGGGT CTGTATCAGT CAGAAGTGAT 
AGGACAGACC CCTTAAGGCA TTTTAAGATT CCAGCGTTTC GGGAAAGACT AGTTAGAGTT 
TCACAACATA CATTCCCTCT CCAAGATAAT TTGCAGGAAC ACCAAGAATG GGTAGACACA 
GAAAAAGAGA GCTTTCAATC TAGTCCTCTA ACTCCAGAAA ATCCATCTTC TCAACAGACA 
GGTTTTTCGT CCTTCAAAGC CTCATTAAGA CTGCCATCGT GTGTGTCCTT GCCATCTGCT 
GACTCTGGTA TCACACAGCA CCCTCTCTCA ACAGAGAGTG ACAGTAAAGT CAAGTCAAGC 
CATCTTCAGA TACCAGAACT GCAGCATAGA CTTTCGAAGA TATCCCAGCT CATCCCGCCT 
CAACAAGACA GTTTGAAGGC CCTTCAAGAA CAATTAGCTA CACAAAGGGA AGCCATCATC 
CACTCTAGAC AAGAAGCTCA TGAAGAAACA CTAAGAGAAT GGAAGGAAAA AATATTCCCA 
GAGCAGGTTG GTCCATTTTC TCCCCTGATA CCACAACATT CACTTGCTTC ATTCCCTGTT 
TCTGATACTG AAAGAGCACA AGAACTGTGT TCAACCAACA GTGACACCAT ATCTTCAGGT 
TATCCTGAGA TGCTGGAGTT GCCTGACAGG ACATTGGGTT TATCACATAC TGCTTTACCT 
CAGCAGAATA ATCTGACTGC ACATCCAGAA CACCTTCATG CACAAACAAA TTTTTTTCAC 
TCTACTGAGA AAGCCCAAGA AGGGTTGGTG TTTCCCAGAC CATGTCAATT TGAAGAGATG 
TCTGCTGAGC ATTTTATCCA GCCTCAACAT GATGACTTAA AGGCACTTCA ACAGCAGTTA 
GATATGCAAA GGGAAGCCAT TAGATCTGGC CAGGAAATGC AAGAGAAAAT GCTTTTGCAA 
AGGTTAAATA AATTGGAGCA AAGGATATCT TCCAAACAAA TTAGCTCCTC TCTATTTTCA 
TCCCAGGTAG CACTGCCCAT TGCCAATTCT GACGGGACCT TACAGTCTTT CCCAACTAAA 
AGTAACGAGA CTGAGCTGCT TGGGTCCCAA GATGAGTATC TGAGCTTTTC ACAGCCTCGT 
CTGCCTCTGC AGAATAACAT GACAGAGCAG TTGGACTTAG AAAAGGTATT TCATAAAGAA 
CTGCTTTTAC ACAAACAAAA GAGCCAAAAC AAAAGTGAAT CTTCTGAGCA TTCCCTCCCT 
CCTTTGTTTC TGTCCAAGGA AATAGAGCAT CCATTCATTT CACTACCTTT TGCAGAAAGT 
AAATCTAAAA GCATTTGTGA GTTGTATTTA TCTGACAAGA AGCATGCAGC TCCTAATGAT 
GCTGTGATCC CAAGATTGCA GGACAGGCTT TTAAGCTGTT CCCAACCTGT CTTAACTCAG 
CAAGATAACA TGAGTCTTCA GAAGCAGCTG AATCTACAAA GAGAAACTCT ACACTCTAGG 
CAAAAAGCCC AAGAAGAATT GCTTGTGCAG AGACAGACAT CCTTACAGCA GCAAATCCAA 
AGACACAGAG AGACTTTGAA AAATTTCTTT AATGTCAGTC AGGCAAGGAA TCCCACAGAT 
GAGAATGACT TGGAAATGCA GAAGAGAGAA CAGCTCGGAG GGTGGTTTCC TCACACCCAA 
GGCCTTACCT GGGGAGACGC AGGTCAGGGG AGCGCTAATG GTGAGCAGCC ACGTGCAGAT 
GTCCATGCTG AGCACAATGG TGAGAGTCTG GCCAAAGAAC TGAGTGGTAG AGCCTCAAAG 
CCACCTGTCT CCAAAGTTAA ATGTGTCTTG GACTTAAACC AGCATGAGCT TAGTACTATA 
CAGGAAGTAG AGTCACCAGC AAGTGGCAGG ATTTCCATGC CAGGTAAACA AGGAAAAGCA 
GAGTTTTATC AAGACCGAGA CCCATTAAGG GTCTCAGTAA GCAGAGAACA AAGTTTCTTG 
GAGAGCCCAC TGGCACATGA TCCATTTGGT TGTCACCAAC CACCTGCCCA AGAGAACAGC 
AAAAGCCATG ACGACAATGC TGAAGCAGTT AAAGTCAAGA AATCTGATGT TGAGGATCAT 
GCAGTATTGA GTCATGCTGT CTCAAAAGAA GAAGCGTGTA CAAACCTGGG TCCACTTGGA 
AAGCCAGACG ATGAGGCTGA AACACAAGAG ATTTCTCAGG AGCCATTGTC TTCAGTAACT 
GTTTCTACTG GAAGCTTCTT AAGTTATGAA ATCACAGATT TGAGCCTCAC AGATCCAGAG 
TCATTTTCAG AACAGACAGA GCATCTAGAA CAAGAATCTA CCAATAAGCA GGAAGAGACT 
GATCCTCTTA GTATTGCAGT CCCTTCAGTT ATTTATCAGC AACAGCATTC CTTAGGTGCT 
CATAACTCTC TGTTGCCCAT GGAGGAAGAA AGTACATCTG ATCATACACA TGTTCAGCAG 
ATCATGGACA ATGATGTAAA TGAAGCAAAT TTGATACCTG ACAAAAGAGA CTTTCAGGTT 
CCAGCTGTGG ACCTTGACTT TCGAGAATTG GAACACATAT TTCCACACTT GCATCGTCAG 
CTCTTTAAAC CCTTAGAACC ACATCTAGAT TTTGACTTAT CATCCCCTGG GACATCTCAA 
GAAGACAGCG ACTTTTACCA GCAGAGCTCA GAATCTTCAT CTGAAAAACA TGTTAAGGCA 
TTATCCACAG GCACAATCTG CTTCCCAGCA CTGACAGCCA AGTCACATTC TCCTAACCCA 
AGGCTGAACC AACTTGATAT TAACCTGGCT CATGCAACAA CTGAGGGATC TGAACAGTCT 
TTTCAACAGC TTCGACCAGA ATTCTCTTCA CAGGAAAGCC AACATGCTGA CCTACCCAGT 
ATTTATAGCA TTGAAGCAAG AGGTCCTTCC CAACGCATGG AAAACCAGAA CTATTCTGAA 
ATGTTACAAA ATAAGAAAAA AAGTCTCTCT CTCCAGCCCT CAACAGAGGA TTTGACTCCA 
GCTTGCAGTT CATCTGATAC TGCTCTGTTT GATCAGTTAC ACCTGCAGCA CAGCACTCCG 
TGTGCTTCTG TCTCTAGTGA GTGCTCAGTA AAACTGTTAG AAAGCAGAGA AGAAGTGCTG 
GGATTTGAAG AGCTGTCAAG AAGGGCAGTT ACAATGTCAC AAAGACTCAC TGAAGATGAA 
AATGTAGTTC TACCTATAAA CCCACATGTA GGAAGAGTGG AGAAGGAGGC TTCAGTTCAG 
GGGTCTAATC CATTGAGTAT TCAAAATGAA AAGCCAATTC AGAACTTCAT TGAAACTGAC 
ACTACAGAAG CTGTAGGAAA TGTATGTCAG CTAGCACAAG CAGAGCACAT ATTAAAATCC 
TGTCCATTTC GGAGCCCTAT TCCAATCTGG GAAACGGATA CTGGCTATGG TATAATGGAA 
GAACCAGATC TCACTCTAGT GAGTAATAGT GACATCAGCA TCACTGAAAC AGATCTTGCA 
AACTTAACCC TTGAAGACAG AGAAGACAAT GAAGCACAGT TTTTTCAGGC AGGTGTCGTT 
CTGCCTACAT CTTCAATGGA AACCTCTGTT TGTGGAGCTG TATCAGAGCC TTATGTGGAC 
CAACCCACAG TGGCACCTTC AGCTACATCA GGTAGTTTAC AAGAAGCATT TATGACAAGA 
CAGACACTTA CAGAACGATC TTACCAGAGG CAGAGAGAGA TCTGGAATAA AACTCGACTC 
CCTCAAACCA AAGTATCCAA AGAGAAGCTA CCTACAGGTT GCACAGGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240852.1
CDS	97..6570
Translation	MEELKQLQKEDLARRRQTVAQMPPQMLELPYRRSEMKEDRQRELEFAFEDMYNADRKVKGNLILHLKPEPLPTISDQLQDEELDLSMEQENQVPLAAKIQQIPSRILFKRLLNKIRSQKSLWTIKSVSEDEGEVTSSIIEIESKVPSVDSGAIITEERTAASFEQEQVTDSDRLTIESGPLSSEDKPLYYKAGTGREQAMAVSPPATAVAQSSVLLHPQEEAVRIRMSLRRKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEQRKQADQPEVCCAPMSHAMISDEDSHRQMIRNYQHQLLQQNRLHKETVETARKRLLEYQTVLKERSPSLSASALVPDSVVSGPPQQSYKPAAASDSWDPSQRLKLSPSKYQPVQPSQIPALEQSHIQVPRHGHITQRQGKMAVSEMLGKQPVESQERQWQFSQVETHQGDYEFVLKDSHSLSRTLSYVRPQTLQDAREVSKPPRVIICQSLDSQQISSEDSENISSKPSEPSPFLPLVPERPFTSLPVKFHSGTIHKPFTTINQSVISQMHDQPLSSSETITAQQGDLRFLQEQLELQKKVLQARQEAREKLLLCTQKELGQQTGLPVFLPSPAGNIFSSLPSASAESGNFQTSSTKSDATVSSDNMDRLWDSSQPISSQQTHLEFLQEQSSVETDNLQARREAQEVLFAHTQNTLEKIVRSEQAGSSLPHQVAQQSFSSLTLADTQSKKIQKQPLPANKKGLLPSQSEVSKAQDGSSGFLQQTLPLQNTLKLLQEQLTRQRSMIPPRRDGQETLLLYKESCSEDSEAGPVESLSSVVVQHADASRAVSEVPKRLQDVYSSEEENRVLSSHLITHGFPQHSLQRQEHFTPLQEETHIQRLILGARKNNEEFAPKQNELEKGLCSQQTDALSSPSQVTDWGTSRGSVSVRSDRTDPLRHFKIPAFRERLVRVSQHTFPLQDNLQEHQEWVDTEKESFQSSPLTPENPSSQQTGFSSFKASLRLPSCVSLPSADSGITQHPLSTESDSKVKSSHLQIPELQHRLSKISQLIPPQQDSLKALQEQLATQREAIIHSRQEAHEETLREWKEKIFPEQVGPFSPLIPQHSLASFPVSDTERAQELCSTNSDTISSGYPEMLELPDRTLGLSHTALPQQNNLTAHPEHLHAQTNFFHSTEKAQEGLVFPRPCQFEEMSAEHFIQPQHDDLKALQQQLDMQREAIRSGQEMQEKMLLQRLNKLEQRISSKQISSSLFSSQVALPIANSDGTLQSFPTKSNETELLGSQDEYLSFSQPRLPLQNNMTEQLDLEKVFHKELLLHKQKSQNKSESSEHSLPPLFLSKEIEHPFISLPFAESKSKSICELYLSDKKHAAPNDAVIPRLQDRLLSCSQPVLTQQDNMSLQKQLNLQRETLHSRQKAQEELLVQRQTSLQQQIQRHRETLKNFFNVSQARNPTDENDLEMQKREQLGGWFPHTQGLTWGDAGQGSANGEQPRADVHAEHNGESLAKELSGRASKPPVSKVKCVLDLNQHELSTIQEVESPASGRISMPGKQGKAEFYQDRDPLRVSVSREQSFLESPLAHDPFGCHQPPAQENSKSHDDNAEAVKVKKSDVEDHAVLSHAVSKEEACTNLGPLGKPDDEAETQEISQEPLSSVTVSTGSFLSYEITDLSLTDPESFSEQTEHLEQESTNKQEETDPLSIAVPSVIYQQQHSLGAHNSLLPMEEESTSDHTHVQQIMDNDVNEANLIPDKRDFQVPAVDLDFRELEHIFPHLHRQLFKPLEPHLDFDLSSPGTSQEDSDFYQQSSESSSEKHVKALSTGTICFPALTAKSHSPNPRLNQLDINLAHATTEGSEQSFQQLRPEFSSQESQHADLPSIYSIEARGPSQRMENQNYSEMLQNKKKSLSLQPSTEDLTPACSSSDTALFDQLHLQHSTPCASVSSECSVKLLESREEVLGFEELSRRAVTMSQRLTEDENVVLPINPHVGRVEKEASVQGSNPLSIQNEKPIQNFIETDTTEAVGNVCQLAQAEHILKSCPFRSPIPIWETDTGYGIMEEPDLTLVSNSDISITETDLANLTLEDREDNEAQFFQAGVVLPTSSMETSVCGAVSEPYVDQPTVAPSATSGSLQEAFMTRQTLTERSYQRQREIWNKTRLPQTKVSKEKLPTGCTGS

Target ORF information:

RefSeq Version	XM_011242550.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 295 (Cep295), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242550.2

ORF Insert Sequence:

ATGGAAGAAC TCAAACAGCT GCAGAAAGAG GACCTGGCCC GTAGGAGGCA GACCGTGGCA 
CAGATGCCGC CACAGATGCT GGAGTTGCCA TACCGACGGA GTGAGATGAA GGAAGACAGG 
CAGCGAGAGC TGGAGTTTGC CTTTGAAGAT ATGTACAATG CAGATAGGAA GGTGAAAGGA 
AACCTGATTT TACATCTTAA GCCAGAACCT TTGCCTACAA TCTCTGATCA GCTCCAAGAT 
GAAGAACTGG ACCTTTCAAT GGAACAAGAA AATCAAGTGC CCTTGGCAGC AAAGATCCAA 
CAAATCCCCT CAAGAATTCT TTTTAAAAGA TTATTAAACA AGATTAGAAG TCAAAAATCT 
CTCTGGACAA TTAAATCTGT GTCTGAGGAT GAAGGTGAGG TGACTTCGAG CATCATTGAG 
ATTGAGAGTA AAGTGCCAAG CGTGGACTCG GGAGCCATTA TCACAGAGGA GAGAACAGCG 
GCGTCCTTTG AGCAGGAGCA AGTCACAGAC AGTGATAGGC TGACGATAGA ATCTGGACCA 
CTGAGCAGTG AAGATAAGCC ATTGTACTAC AAAGCGGGGA CTGGAAGGGA ACAAGCAATG 
GCAGTGTCTC CACCTGCCAC AGCTGTGGCT CAGAGTTCAG TCCTGCTTCA TCCTCAGGAA 
GAGGCAGTCA GAATTCGAAT GTCATTAAGG CGCAAACAGA TAATGGAGAT AGAAGAGCAG 
AAGCAGAAGC AGTTGGAATT ACTTGAACAA ATTGAGCAAC AGAAGTTAAG GTTAGAAACT 
GATTGTTTCC GGGCTCAACT GGAAGAGCAA AGAAAGCAAG CTGATCAGCC TGAGGTTTGC 
TGTGCTCCAA TGTCACATGC TATGATTTCT GATGAAGACT CTCACAGGCA GATGATTCGT 
AACTATCAAC ATCAGCTTTT ACAACAAAAC AGGTTACACA AGGAGACTGT TGAAACTGCC 
AGGAAACGAT TACTCGAATA CCAAACTGTA TTAAAAGAAA GATCCCCATC CCTGTCAGCC 
TCAGCACTGG TACCTGATTC TGTTGTGTCA GGGCCACCAC AGCAATCCTA CAAGCCTGCT 
GCTGCTTCAG ACTCTTGGGA TCCAAGCCAG AGACTGAAAC TAAGTCCTAG CAAATATCAA 
CCTGTGCAAC CCTCACAGAT CCCTGCATTA GAGCAGAGTC ATATTCAGGT ACCAAGACAT 
GGTCACATTA CACAGAGGCA AGGAAAAATG GCTGTATCAG AAATGTTAGG TAAGCAGCCC 
GTGGAGTCAC AAGAACGTCA GTGGCAGTTC TCTCAGGTGG AAACACATCA GGGAGACTAT 
GAGTTCGTCC TTAAAGATTC TCATTCACTT TCAAGGACTT TGTCTTATGT TAGGCCACAA 
ACATTACAAG ATGCTAGAGA AGTATCTAAA CCTCCTAGGG TAATAATTTG TCAAAGTCTA 
GATTCCCAAC AGATATCATC AGAGGATAGT GAAAATATAT CTTCTAAGCC AAGTGAACCG 
TCTCCTTTTC TCCCACTGGT GCCTGAACGC CCTTTTACTA GTCTCCCAGT TAAATTCCAT 
TCTGGAACAA TTCACAAACC CTTTACGACC ATAAACCAGT CTGTAATCAG TCAAATGCAT 
GATCAACCTT TGTCATCCTC AGAGACTATT ACAGCTCAGC AGGGTGACTT AAGATTTCTT 
CAAGAACAGT TAGAACTACA GAAGAAAGTT CTTCAGGCAA GACAAGAGGC TCGGGAAAAG 
CTGCTTTTGT GCACACAGAA AGAACTGGGA CAGCAGACTG GCCTCCCAGT GTTCCTCCCT 
TCACCAGCTG GAAATATATT TTCTTCATTA CCCTCTGCCT CAGCTGAATC AGGAAACTTT 
CAGACATCTT CAACAAAAAG TGATGCCACT GTTTCCTCAG ACAATATGGA CAGGCTTTGG 
GATTCTTCAC AACCCATCTC ATCACAGCAA ACTCATTTAG AATTTCTCCA AGAGCAGTCC 
AGTGTTGAGA CGGATAACCT TCAGGCTAGG CGGGAAGCCC AGGAGGTCTT GTTTGCACAT 
ACACAAAATA CGCTGGAAAA AATTGTACGT TCTGAACAAG CTGGGTCTTC TTTGCCACAT 
CAGGTAGCTC AGCAGTCATT TAGTTCACTA ACATTGGCTG ATACACAATC TAAAAAAATC 
CAGAAACAGC CTTTACCTGC AAACAAGAAA GGGCTACTCC CAAGCCAGTC TGAAGTCTCA 
AAAGCTCAGG ATGGATCTTC AGGTTTTCTA CAGCAGACCC TACCTCTCCA GAACACTTTA 
AAGTTGCTTC AAGAGCAGCT GACTAGACAG AGGAGTATGA TTCCGCCTAG ACGTGATGGT 
CAAGAGACAT TACTTTTATA TAAAGAAAGC TGTTCAGAAG ACAGTGAGGC TGGGCCAGTA 
GAATCACTCT CCTCCGTGGT TGTTCAGCAT GCAGATGCTT CTCGTGCAGT TTCAGAAGTA 
CCTAAGCGGC TCCAGGACGT GTATTCATCT GAGGAAGAAA ATAGAGTTCT CTCCAGTCAT 
TTGATCACCC ATGGCTTTCC ACAGCACAGC CTGCAAAGAC AAGAACATTT TACACCACTC 
CAGGAAGAGA CACACATTCA GAGGCTTATA CTTGGTGCTA GAAAAAATAA TGAGGAATTT 
GCTCCCAAGC AAAATGAATT AGAAAAGGGG CTCTGCTCTC AACAGACTGA TGCCTTGTCT 
TCTCCATCCC AAGTCACCGA CTGGGGAACA TCCCGGGGGT CTGTATCAGT CAGAAGTGAT 
AGGACAGACC CCTTAAGGCA TTTTAAGATT CCAGCGTTTC GGGAAAGACT AGTTAGAGTT 
TCACAACATA CATTCCCTCT CCAAGATAAT TTGCAGGAAC ACCAAGAATG GGTAGACACA 
GAAAAAGAGA GCTTTCAATC TAGTCCTCTA ACTCCAGAAA ATCCATCTTC TCAACAGACA 
GGTTTTTCGT CCTTCAAAGC CTCATTAAGA CTGCCATCGT GTGTGTCCTT GCCATCTGCT 
GACTCTGGTA TCACACAGCA CCCTCTCTCA ACAGAGAGTG ACAGTAAAGT CAAGTCAAGC 
CATCTTCAGA TACCAGAACT GCAGCATAGA CTTTCGAAGA TATCCCAGCT CATCCCGCCT 
CAACAAGACA GTTTGAAGGC CCTTCAAGAA CAATTAGCTA CACAAAGGGA AGCCATCATC 
CACTCTAGAC AAGAAGCTCA TGAAGAAACA CTAAGAGAAT GGAAGGAAAA AATATTCCCA 
GAGCAGGTTG GTCCATTTTC TCCCCTGATA CCACAACATT CACTTGCTTC ATTCCCTGTT 
TCTGATACTG AAAGAGCACA AGAACTGTGT TCAACCAACA GTGACACCAT ATCTTCAGGT 
TATCCTGAGA TGCTGGAGTT GCCTGACAGG ACATTGGGTT TATCACATAC TGCTTTACCT 
CAGCAGAATA ATCTGACTGC ACATCCAGAA CACCTTCATG CACAAACAAA TTTTTTTCAC 
TCTACTGAGA AAGCCCAAGA AGGGTTGGTG TTTCCCAGAC CATGTCAATT TGAAGAGATG 
TCTGCTGAGC ATTTTATCCA GCCTCAACAT GATGACTTAA AGGCACTTCA ACAGCAGTTA 
GATATGCAAA GGGAAGCCAT TAGATCTGGC CAGGAAATGC AAGAGAAAAT GCTTTTGCAA 
AGGTTAAATA AATTGGAGCA AAGGATATCT TCCAAACAAA TTAGCTCCTC TCTATTTTCA 
TCCCAGGTAG CACTGCCCAT TGCCAATTCT GACGGGACCT TACAGTCTTT CCCAACTAAA 
AGTAACGAGA CTGAGCTGCT TGGGTCCCAA GATGAGTATC TGAGCTTTTC ACAGCCTCGT 
CTGCCTCTGC AGAATAACAT GACAGAGCAG TTGGACTTAG AAAAGGTATT TCATAAAGAA 
CTGCTTTTAC ACAAACAAAA GAGCCAAAAC AAAAGTGAAT CTTCTGAGCA TTCCCTCCCT 
CCTTTGTTTC TGTCCAAGGA AATAGAGCAT CCATTCATTT CACTACCTTT TGCAGAAAGT 
AAATCTAAAA GCATTTGTGA GTTGTATTTA TCTGACAAGA AGCATGCAGC TCCTAATGAT 
GCTGTGATCC CAAGATTGCA GGACAGGCTT TTAAGCTGTT CCCAACCTGT CTTAACTCAG 
CAAGATAACA TGAGTCTTCA GAAGCAGCTG AATCTACAAA GAGAAACTCT ACACTCTAGG 
CAAAAAGCCC AAGAAGAATT GCTTGTGCAG AGACAGACAT CCTTACAGCA GCAAATCCAA 
AGACACAGAG AGACTTTGAA AAATTTCTTT AATGTCAGTC AGGCAAGGAA TCCCACAGAT 
GAGAATGACT TGGAAATGCA GAAGAGAGAA CAGCTCGGAG GGTGGTTTCC TCACACCCAA 
GGCCTTACCT GGGGAGACGC AGGTCAGGGG AGCGCTAATG GTGAGCAGCC ACGTGCAGAT 
GTCCATGCTG AGCACAATGG TGAGAGTCTG GCCAAAGAAC TGAGTGGTAG AGCCTCAAAG 
CCACCTGTCT CCAAAGTTAA ATGTGTCTTG GACTTAAACC AGCATGAGCT TAGTACTATA 
CAGGAAGTAG AGTCACCAGC AAGTGGCAGG ATTTCCATGC CAGGTAAACA AGGAAAAGCA 
GAGTTTTATC AAGACCGAGA CCCATTAAGG GTCTCAGTAA GCAGAGAACA AAGTTTCTTG 
GAGAGCCCAC TGGCACATGA TCCATTTGGT TGTCACCAAC CACCTGCCCA AGAGAACAGC 
AAAAGCCATG ACGACAATGC TGAAGCAGTT AAAGTCAAGA AATCTGATGT TGAGGATCAT 
GCAGTATTGA GTCATGCTGT CTCAAAAGAA GAAGCGTGTA CAAACCTGGG TCCACTTGGA 
AAGCCAGACG ATGAGGCTGA AACACAAGAG ATTTCTCAGG AGCCATTGTC TTCAGTAACT 
GTTTCTACTG GAAGCTTCTT AAGTTATGAA ATCACAGATT TGAGCCTCAC AGATCCAGAG 
TCATTTTCAG AACAGACAGA GCATCTAGAA CAAGAATCTA CCAATAAGCA GGAAGAGACT 
GATCCTCTTA GTATTGCAGT CCCTTCAGTT ATTTATCAGC AACAGCATTC CTTAGGTGCT 
CATAACTCTC TGTTGCCCAT GGAGGAAGAA AGTACATCTG ATCATACACA TGTTCAGCAG 
ATCATGGACA ATGATGTAAA TGAAGCAAAT TTGATACCTG ACAAAAGAGA CTTTCAGGTT 
CCAGCTGTGG ACCTTGACTT TCGAGAATTG GAACACATAT TTCCACACTT GCATCGTCAG 
CTCTTTAAAC CCTTAGAACC ACATCTAGAT TTTGACTTAT CATCCCCTGG GACATCTCAA 
GAAGACAGCG ACTTTTACCA GCAGAGCTCA GAATCTTCAT CTGAAAAACA TGTTAAGGCA 
TTATCCACAG GCACAATCTG CTTCCCAGCA CTGACAGCCA AGTCACATTC TCCTAACCCA 
AGGCTGAACC AACTTGATAT TAACCTGGCT CATGCAACAA CTGAGGGATC TGAACAGTCT 
TTTCAACAGC TTCGACCAGA ATTCTCTTCA CAGGAAAGCC AACATGCTGA CCTACCCAGT 
ATTTATAGCA TTGAAGCAAG AGGTCCTTCC CAACGCATGG AAAACCAGAA CTATTCTGAA 
ATGTTACAAA ATAAGAAAAA AAGTCTCTCT CTCCAGCCCT CAACAGAGGA TTTGACTCCA 
GCTTGCAGTT CATCTGATAC TGCTCTGTTT GATCAGTTAC ACCTGCAGCA CAGCACTCCG 
TGTGCTTCTG TCTCTAGTGA GTGCTCAGTA AAACTGTTAG AAAGCAGAGA AGAAGTGCTG 
GGATTTGAAG AGCTGTCAAG AAGGGCAGTT ACAATGTCAC AAAGACTCAC TGAAGATGAA 
AATGTAGTTC TACCTATAAA CCCACATGTA GGAAGAGTGG AGAAGGAGGC TTCAGTTCAG 
GGGTCTAATC CATTGAGTAT TCAAAATGAA AAGCCAATTC AGAACTTCAT TGAAACTGAC 
ACTACAGAAG CTGTAGGAAA TGTATGTCAG CTAGCACAAG CAGAGCACAT ATTAAAATCC 
TGTCCATTTC GGAGCCCTAT TCCAATCTGG GAAACGGATA CTGGCTATGG TATAATGGAA 
GAACCAGATC TCACTCTAGT GAGTAATAGT GACATCAGCA TCACTGAAAC AGATCTTGCA 
AACTTAACCC TTGAAGACAG AGAAGACAAT GAAGCACAGT TTTTTCAGGC AGGTGTCGTT 
CTGCCTACAT CTTCAATGGA AACCTCTGTT TGTGGAGCTG TATCAGAGCC TTATGTGGAC 
CAACCCACAG TGGCACCTTC AGCTACATCA GGTAGTTTAC AAGAAGCATT TATGACAAGA 
CAGACACTTA CAGAACGATC TTACCAGAGG CAGAGAGAGA TCTGGAATAA AACTCGACTC 
CCTCAAACCA AAGTATCCAA AGAGAAGCTA CCTACAGGTT GCACAGGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Cep295
Entrez Gene ID	319675
Full Name	centrosomal protein 295
Synonyms	5830418K08Rik,5832426L23Rik,Gm1131,Kiaa1731
General protein information	Preferred Names centrosomal protein 295 Names centrosomal protein of 295 kDa
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A2

Homo sapiens (human)	KIAA1731	NP_203753.1
Pan troglodytes (chimpanzee)	KIAA1731	XP_508695.3
Bos taurus (cattle)	KIAA1731	XP_003588053.2
Mus musculus (house mouse)	5830418K08Rik	NP_795950.2
	LOC697392	XP_001085979.2
Rattus norvegicus (Norway rat)	RGD1311723	NP_001257977.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Cep295 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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