Hif1an cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hif1an
Entrez Gene ID	319594
Full Name	hypoxia-inducible factor 1, alpha subunit inhibitor
Synonyms	2310046M24Rik,A830014H24Rik,FIH,FIH1
General protein information	Preferred Names hypoxia-inducible factor 1, alpha subunit inhibitor Names hypoxia-inducible factor 1-alpha inhibitor hypoxia-inducible factor asparagine hydroxylase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_176958.3	NP_795932.2	hypoxia-inducible factor 1-alpha inhibitor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Hif1an	NP_795932.2
Xenopus tropicalis (tropical clawed frog)	hif1an	NP_988915.1
Pan troglodytes (chimpanzee)	HIF1AN	XP_521585.2
Rattus norvegicus (Norway rat)	Hif1an	NP_001107221.1
Gallus gallus (chicken)	HIF1AN	XP_426507.2
Danio rerio (zebrafish)	hif1an	NP_958904.1
Homo sapiens (human)	HIF1AN	NP_060372.2
	HIF1AN	XP_001108283.2
Canis lupus familiaris (dog)	HIF1AN	XP_851531.2
Bos taurus (cattle)	HIF1AN	NP_001076912.1

Gene Ontology
Bibliography

Related articles in PubMed

The role of the miR-31/FIH1 pathway in TGF-?-induced liver fibrosis.
Hu J, Chen C, Liu Q, Liu B, Song C, Zhu S, Wu C, Liu S, Yu H, Yao D, Kang J, Zhu L
Clinical science (London, England : 1979)129(4)305-17(2015 Aug)

Anks3 interacts with nephronophthisis proteins and is required for normal renal development.
Yakulov TA, Yasunaga T, Ramachandran H, Engel C, M?ller B, Hoff S, Dengjel J, Lienkamp SS, Walz G
Kidney international87(6)1191-200(2015 Jun)

FIH-1 disrupts an LRRK1/EGFR complex to positively regulate keratinocyte migration.
Peng H, Kaplan N, Yang W, Getsios S, Lavker RM
The American journal of pathology184(12)3262-71(2014 Dec)

A gain-of-function mutation in Tnni2 impeded bone development through increasing Hif3a expression in DA2B mice.
Zhu X, Wang F, Zhao Y, Yang P, Chen J, Sun H, Liu L, Li W, Pan L, Guo Y, Kou Z, Zhang Y, Zhou C, He J, Zhang X, Li J, Han W, Li J, Liu G, Gao S, Yang Z
PLoS genetics10(10)e1004589(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-31/FIH1 pathway associates with liver fibrosis, perhaps by participation in the TGF-beta/Smad3 signalling of hepatic stellate cells.
Title: The role of the miR-31/FIH1 pathway in TGF-?-induced liver fibrosis.

FIH-1 has a role in disrupting the LRRK1/EGFR complex to positively regulate keratinocyte migration
Title: FIH-1 disrupts an LRRK1/EGFR complex to positively regulate keratinocyte migration.

gankyrin binds to and sequester factor inhibiting hypoxia-inducible factor-1 (FIH-1), which results in decreased interaction between FIH-1 and hypoxia-inducible factor-1alpha (HIF-1alpha) and increased activity of HIF-1 to promote VEGF production.
Title: Overexpression of gankyrin in mouse hepatocytes induces hemangioma by suppressing factor inhibiting hypoxia-inducible factor-1 (FIH-1) and activating hypoxia-inducible factor-1.

Our results define a previously unknown mechanism for keratinocyte fate decisions where Notch signaling potential is, in part, controlled through a miR-31/FIH-1 nexus.
Title: microRNA-31/factor-inhibiting hypoxia-inducible factor 1 nexus regulates keratinocyte differentiation.

FIH recognizes distinct molecular features within HIF-alpha1 versus ankyrin repeat containing substrates Notch1/4 and Gankyrin.
Title: Factor inhibiting HIF (FIH) recognizes distinct molecular features within hypoxia-inducible factor-? (HIF-?) versus ankyrin repeat substrates.

The following Hif1an gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hif1an cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu23399	NM_176958.3 Latest version!	Mus musculus hypoxia-inducible factor 1, alpha subunit inhibitor (Hif1an), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$237.30 $339.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hif1an ( NM_176958.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_795932.2 Mus musculus hypoxia-inducible factor 1, alpha subunit inhibitor (Hif1an), mRNA.

Price & Availability↑

CloneID	OMu23399
Clone ID Related Accession (Same CDS sequence)	NM_176958.3
Accession Version	NM_176958.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	hypoxia-inducible factor 1-alpha inhibitor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY122345.1, BY713373.1, CN535711.1 and AC151478.1. On Jul 16, 2005 this sequence version replaced NM_176958.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK154774.1, SRR1660819.47759.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGA CGGCAGCCGA GGTTGCGGCC TCTGGCTCTG GAGAGGCCCG GGAAGAAGCC 
GAAGCTCCGG GTCCCGCCTG GGATGAGTCC CAGCTACGAA GTTACAGCTT TCCGACCAGG 
CCCATCCCAC GTCTGAGCCA GAGTGACCCT CGGGCGGAAG AGCTCATCGA AAATGAGGAG 
CCAGTGGTGC TGACCGACAC AAACCTTGTG TATCCTGCAC TGAAGTGGGA CCTCGAATAC 
CTGCAAGAGA ATATTGGCAA CGGAGATTTC TCTGTGTACA GTGCCAGCAC CCATAAGTTC 
TTATACTATG ATGAGAAGAA GATGGGCAAT TTCCAGAACT TTAAGCCAAG GTCCAACAGG 
GAGGAAATTA AATTTCATGA GTTCGTTGAG AAACTACAAG CTATACAGCA GCGCGGGGGA 
GAAGAGCGGT TGTATCTGCA GCAGACACTC AATGACACCG TGGGGAGGAA GATTGTCATG 
GACTTCTTGG GTTTTAACTG GAACTGGATC AATAAACAGC AGGGGAAACG CGGCTGGGGG 
CAGCTGACCT CTAACCTGTT GCTCATTGGC ATGGAAGGAA ATGTGACACC TGCTCACTAT 
GATGAGCAGC AGAACTTTTT TGCCCAAATA AAAGGCCATA AGAGATGCAT CTTATTTCCT 
CCGGATCAGT TCGAGTGCCT CTATCCATAC CCTGTCCATC ACCCCTGTGA CAGGCAGAGC 
CAGGTGGACT TTGACAATCC TGACTACGAG AGGTTCCCCA ATTTCCGAAA TGTGGTTGGT 
TATGAGACAG TGGTTGGCCC TGGGGATGTT CTTTACATCC CAATGTACTG GTGGCACCAT 
ATAGAGTCAT TACTAAATGG GGGAATTACC ATCACTGTGA ACTTCTGGTA TAAGGGGGCT 
CCCACCCCTA AGAGAATCGA ATATCCTCTC AAAGCTCATC AGAAAGTAGC CATCATGAGA 
AACATTGAGA AGATGCTTGG AGAGGCCTTG GGGAACCCAC AAGAGGTGGG GCCTTTGTTG 
AACACCATGA TTAAAGGCCG TTACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_795932.2
CDS	25..1074
Translation	MAATAAEVAASGSGEAREEAEAPGPAWDESQLRSYSFPTRPIPRLSQSDPRAEELIENEEPVVLTDTNLVYPALKWDLEYLQENIGNGDFSVYSASTHKFLYYDEKKMGNFQNFKPRSNREEIKFHEFVEKLQAIQQRGGEERLYLQQTLNDTVGRKIVMDFLGFNWNWINKQQGKRGWGQLTSNLLLIGMEGNVTPAHYDEQQNFFAQIKGHKRCILFPPDQFECLYPYPVHHPCDRQSQVDFDNPDYERFPNFRNVVGYETVVGPGDVLYIPMYWWHHIESLLNGGITITVNFWYKGAPTPKRIEYPLKAHQKVAIMRNIEKMLGEALGNPQEVGPLLNTMIKGRYN

Target ORF information:

RefSeq Version	NM_176958.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypoxia-inducible factor 1, alpha subunit inhibitor (Hif1an), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176958.3

ORF Insert Sequence:

ATGGCGGCGA CGGCAGCCGA GGTTGCGGCC TCTGGCTCTG GAGAGGCCCG GGAAGAAGCC 
GAAGCTCCGG GTCCCGCCTG GGATGAGTCC CAGCTACGAA GTTACAGCTT TCCGACCAGG 
CCCATCCCAC GTCTGAGCCA GAGTGACCCT CGGGCGGAAG AGCTCATCGA AAATGAGGAG 
CCAGTGGTGC TGACCGACAC AAACCTTGTG TATCCTGCAC TGAAGTGGGA CCTCGAATAC 
CTGCAAGAGA ATATTGGCAA CGGAGATTTC TCTGTGTACA GTGCCAGCAC CCATAAGTTC 
TTATACTATG ATGAGAAGAA GATGGGCAAT TTCCAGAACT TTAAGCCAAG GTCCAACAGG 
GAGGAAATTA AATTTCATGA GTTCGTTGAG AAACTACAAG CTATACAGCA GCGCGGGGGA 
GAAGAGCGGT TGTATCTGCA GCAGACACTC AATGACACCG TGGGGAGGAA GATTGTCATG 
GACTTCTTGG GTTTTAACTG GAACTGGATC AATAAACAGC AGGGGAAACG CGGCTGGGGG 
CAGCTGACCT CTAACCTGTT GCTCATTGGC ATGGAAGGAA ATGTGACACC TGCTCACTAT 
GATGAGCAGC AGAACTTTTT TGCCCAAATA AAAGGCCATA AGAGATGCAT CTTATTTCCT 
CCGGATCAGT TCGAGTGCCT CTATCCATAC CCTGTCCATC ACCCCTGTGA CAGGCAGAGC 
CAGGTGGACT TTGACAATCC TGACTACGAG AGGTTCCCCA ATTTCCGAAA TGTGGTTGGT 
TATGAGACAG TGGTTGGCCC TGGGGATGTT CTTTACATCC CAATGTACTG GTGGCACCAT 
ATAGAGTCAT TACTAAATGG GGGAATTACC ATCACTGTGA ACTTCTGGTA TAAGGGGGCT 
CCCACCCCTA AGAGAATCGA ATATCCTCTC AAAGCTCATC AGAAAGTAGC CATCATGAGA 
AACATTGAGA AGATGCTTGG AGAGGCCTTG GGGAACCCAC AAGAGGTGGG GCCTTTGTTG 
AACACCATGA TTAAAGGCCG TTACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The role of the miR-31/FIH1 pathway in TGF-?-induced liver fibrosis.
Clinical science (London, England : 1979)129(4)305-17(2015 Aug)
Hu J,Chen C,Liu Q,Liu B,Song C,Zhu S,Wu C,Liu S,Yu H,Yao D,Kang J,Zhu L

Anks3 interacts with nephronophthisis proteins and is required for normal renal development.
Kidney international87(6)1191-200(2015 Jun)
Yakulov TA,Yasunaga T,Ramachandran H,Engel C,M?ller B,Hoff S,Dengjel J,Lienkamp SS,Walz G

FIH-1 disrupts an LRRK1/EGFR complex to positively regulate keratinocyte migration.
The American journal of pathology184(12)3262-71(2014 Dec)
Peng H,Kaplan N,Yang W,Getsios S,Lavker RM

A gain-of-function mutation in Tnni2 impeded bone development through increasing Hif3a expression in DA2B mice.
PLoS genetics10(10)e1004589(2014 Oct)
Zhu X,Wang F,Zhao Y,Yang P,Chen J,Sun H,Liu L,Li W,Pan L,Guo Y,Kou Z,Zhang Y,Zhou C,He J,Zhang X,Li J,Han W,Li J,Liu G,Gao S,Yang Z

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Hif1an cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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