Gpr4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpr4
Entrez Gene ID	319197
Full Name	G protein-coupled receptor 4
General protein information	Preferred Names G protein-coupled receptor 4 Names G-protein coupled receptor 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006540048.2	XP_006540111.1	G-protein coupled receptor 4 isoform X1
XM_006540048.3	XP_006540111.1	G-protein coupled receptor 4 isoform X1
NM_175668.4	NP_783599.1	G-protein coupled receptor 4

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPR4	NP_005273.1
	LOC716148	XP_001106715.2
Canis lupus familiaris (dog)	GPR4	XP_005616498.1
Mus musculus (house mouse)	Gpr4	NP_783599.1
Bos taurus (cattle)	GPR4	NP_001069051.1
Rattus norvegicus (Norway rat)	Gpr4	NP_001020851.1
Danio rerio (zebrafish)	LOC563762	XP_005157731.1

Gene Ontology
Bibliography

Related articles in PubMed

GPR4 knockout improves renal ischemia-reperfusion injury and inhibits apoptosis via suppressing the expression of CHOP.
Dong B, Zhang X, Fan Y, Cao S, Zhang X
The Biochemical journal474(24)4065-4074(2017 12)

GPR4 deficiency alleviates intestinal inflammation in a mouse model of acute experimental colitis.
Sanderlin EJ, Leffler NR, Lertpiriyapong K, Cai Q, Hong H, Bakthavatchalu V, Fox JG, Oswald JZ, Justus CR, Krewson EA, O'Rourke D, Yang LV
Biochimica et biophysica acta1863(2)569-584(2017 Feb)

Deletion of proton-sensing receptor GPR4 associates with lower blood pressure and lower binding of angiotensin II receptor in SFO.
Sun X, Tommasi E, Molina D, Sah R, Brosnihan KB, Diz D, Petrovic S
American journal of physiology. Renal physiology311(6)F1260-F1266(2016 Dec)

Acidic environment augments Fc?RI-mediated production of IL-6 and IL-13 in mast cells.
Kamide Y, Ishizuka T, Tobo M, Tsurumaki H, Aoki H, Mogi C, Nakakura T, Yatomi M, Ono A, Koga Y, Sato K, Hisada T, Dobashi K, Yamada M, Okajima F
Biochemical and biophysical research communications464(3)949-55(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GPR4 blockade attenuated renal injury after IR and reduced the cell apoptosis through the suppression of CHOP expression.
Title: GPR4 knockout improves renal ischemia-reperfusion injury and inhibits apoptosis via suppressing the expression of CHOP.

Collectively, these results posit the acid sensor GPR4 as a novel component of central blood pressure control through interactions with the renin-angiotensin system.
Title: Deletion of proton-sensing receptor GPR4 associates with lower blood pressure and lower binding of angiotensin II receptor in SFO.

required for maintenance of normal acid-base homeostasis
Title: Adaptation by the collecting duct to an exogenous acid load is blunted by deletion of the proton-sensing receptor GPR4.

The data identify GPR4 and TASK-2 as distinct, parallel, and essential central mediators of respiratory chemosensitivity.
Title: PHYSIOLOGY. Regulation of breathing by CO? requires the proton-activated receptor GPR4 in retrotrapezoid nucleus neurons.

The results indicate that through the G12/13/Rho signaling pathway GPR4 modulates focal adhesion dynamics and reduces cell spreading and membrane ruffling.
Title: GPR4 decreases B16F10 melanoma cell spreading and regulates focal adhesion dynamics through the G13/Rho signaling pathway.

The following Gpr4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpr4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05694	NM_175668.4 Latest version!	Mus musculus G protein-coupled receptor 4 (Gpr4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu05694	XM_006540048.3 Latest version!	Mus musculus G protein-coupled receptor 4 (Gpr4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
View Old Accession Versions >>
OMu05694	XM_006540048.2	Mus musculus G protein-coupled receptor 4 (Gpr4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpr4 ( NM_175668.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_783599.1 Mus musculus G protein-coupled receptor 4 (Gpr4), mRNA.

Price & Availability↑

CloneID	OMu05694
Clone ID Related Accession (Same CDS sequence)	XM_006540048.2 , XM_006540048.3 , NM_175668.4
Accession Version	NM_175668.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	G-protein coupled receptor 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC148988.5. On Jul 30, 2009 this sequence version replaced NM_175668.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK085829.1, BB661993.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGACAACA GCACGGGCAC AGGGGAGGGC TGCCATGTGG ACTCTCGAGT GGACCACCTC 
TTCCCACCAT CTCTCTACAT CTTCGTCATC GGGGTGGGGC TGCCCACCAA CTGCCTGGCC 
CTGTGGGCAG CCTACCGGCA GGTGCGCCAA CACAATGAGC TGGGCGTCTA CCTGATGAAC 
TTGAGCATTG CAGACCTGCT GTACATCTGC ACTTTGCCGC TGTGGGTCGA CTACTTCCTC 
CACCATGACA ACTGGATCCA CGGCCCTGGC TCCTGCAAGC TCTTTGGCTT CATCTTCTAC 
AGCAACATCT ATATCAGCAT CGCCTTCCTG TGCTGCATCT CCGTGGACCG CTACCTGGCT 
GTGGCTCATC CTCTGCGCTT TGCACGCCTG CGCCGGGTCA AGACAGCAGT GGCTGTGAGC 
TCTGTGGTCT GGGCCACGGA GCTGGGCGCC AATTCAGCAC CGCTCTTCCA TGATGAGCTG 
TTTCGTGATC GCTACAACCA CACCTTCTGC TTTGAGAAGT TCCCCATGGA GCGTTGGGTG 
GCCTGGATGA ATCTGTACCG CGTCTTTGTG GGCTTCCTCT TCCCCTGGGC ACTCATGTTG 
CTGTGCTACC GTGGCATCCT GAGGGCAGTG CAGAGCAGTG TGTCCACCGA GCGCCAGGAG 
AAAGTCAAGA TCAAACGTCT GGCCCTGAGC CTCATCGCCA TTGTGCTGGT GTGCTTTGCG 
CCTTACCATG CTCTCCTGCT GTCTCGCAGC GCCGTCTACC TGGGCCGGCC CTGGGACTGT 
GGCTTCGAGG AGCGAGTCTT TTCTGCCTAC CACAGCTCCC TGGCCTTCAC CAGCCTCAAT 
TGTGTGGCTG ACCCCATCCT CTACTGCCTG GTCAACGAGG GTGCCCGCAG TGATGTGGCC 
AAGGCCCTGC ACAACCTCCT CCGCTTCCTG GCCAGCAACA AGCCCCAGGA GATGGCCAAT 
GCTTCCCTCA CCCTGGAGAC ACCCTTGACC TCCAAGAGGA GCACCACCGG CAAGTCGTCC 
GGGGCTGTCT GGGCAGTGCC TCCGACTGCC CAGGGGGACC AGGTGCCACT GAAGGTGCTG 
CTGCCCCCGG CACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_783599.1
CDS	863..1960
Translation	MDNSTGTGEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVRQHNELGVYLMNLSIADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYSNIYISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMERWVAWMNLYRVFVGFLFPWALMLLCYRGILRAVQSSVSTERQEKVKIKRLALSLIAIVLVCFAPYHALLLSRSAVYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASNKPQEMANASLTLETPLTSKRSTTGKSSGAVWAVPPTAQGDQVPLKVLLPPAQ

Target ORF information:

RefSeq Version	NM_175668.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 4 (Gpr4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175668.4

ORF Insert Sequence:

ATGGACAACA GCACGGGCAC AGGGGAGGGC TGCCATGTGG ACTCTCGAGT GGACCACCTC 
TTCCCACCAT CTCTCTACAT CTTCGTCATC GGGGTGGGGC TGCCCACCAA CTGCCTGGCC 
CTGTGGGCAG CCTACCGGCA GGTGCGCCAA CACAATGAGC TGGGCGTCTA CCTGATGAAC 
TTGAGCATTG CAGACCTGCT GTACATCTGC ACTTTGCCGC TGTGGGTCGA CTACTTCCTC 
CACCATGACA ACTGGATCCA CGGCCCTGGC TCCTGCAAGC TCTTTGGCTT CATCTTCTAC 
AGCAACATCT ATATCAGCAT CGCCTTCCTG TGCTGCATCT CCGTGGACCG CTACCTGGCT 
GTGGCTCATC CTCTGCGCTT TGCACGCCTG CGCCGGGTCA AGACAGCAGT GGCTGTGAGC 
TCTGTGGTCT GGGCCACGGA GCTGGGCGCC AATTCAGCAC CGCTCTTCCA TGATGAGCTG 
TTTCGTGATC GCTACAACCA CACCTTCTGC TTTGAGAAGT TCCCCATGGA GCGTTGGGTG 
GCCTGGATGA ATCTGTACCG CGTCTTTGTG GGCTTCCTCT TCCCCTGGGC ACTCATGTTG 
CTGTGCTACC GTGGCATCCT GAGGGCAGTG CAGAGCAGTG TGTCCACCGA GCGCCAGGAG 
AAAGTCAAGA TCAAACGTCT GGCCCTGAGC CTCATCGCCA TTGTGCTGGT GTGCTTTGCG 
CCTTACCATG CTCTCCTGCT GTCTCGCAGC GCCGTCTACC TGGGCCGGCC CTGGGACTGT 
GGCTTCGAGG AGCGAGTCTT TTCTGCCTAC CACAGCTCCC TGGCCTTCAC CAGCCTCAAT 
TGTGTGGCTG ACCCCATCCT CTACTGCCTG GTCAACGAGG GTGCCCGCAG TGATGTGGCC 
AAGGCCCTGC ACAACCTCCT CCGCTTCCTG GCCAGCAACA AGCCCCAGGA GATGGCCAAT 
GCTTCCCTCA CCCTGGAGAC ACCCTTGACC TCCAAGAGGA GCACCACCGG CAAGTCGTCC 
GGGGCTGTCT GGGCAGTGCC TCCGACTGCC CAGGGGGACC AGGTGCCACT GAAGGTGCTG 
CTGCCCCCGG CACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr4 ( XM_006540048.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540111.1 Mus musculus G protein-coupled receptor 4 (Gpr4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu05694
Clone ID Related Accession (Same CDS sequence)	XM_006540048.2 , XM_006540048.3 , NM_175668.4
Accession Version	XM_006540048.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	G-protein coupled receptor 4 isoform X1
Comment

ATGGACAACA GCACGGGCAC AGGGGAGGGC TGCCATGTGG ACTCTCGAGT GGACCACCTC 
TTCCCACCAT CTCTCTACAT CTTCGTCATC GGGGTGGGGC TGCCCACCAA CTGCCTGGCC 
CTGTGGGCAG CCTACCGGCA GGTGCGCCAA CACAATGAGC TGGGCGTCTA CCTGATGAAC 
TTGAGCATTG CAGACCTGCT GTACATCTGC ACTTTGCCGC TGTGGGTCGA CTACTTCCTC 
CACCATGACA ACTGGATCCA CGGCCCTGGC TCCTGCAAGC TCTTTGGCTT CATCTTCTAC 
AGCAACATCT ATATCAGCAT CGCCTTCCTG TGCTGCATCT CCGTGGACCG CTACCTGGCT 
GTGGCTCATC CTCTGCGCTT TGCACGCCTG CGCCGGGTCA AGACAGCAGT GGCTGTGAGC 
TCTGTGGTCT GGGCCACGGA GCTGGGCGCC AATTCAGCAC CGCTCTTCCA TGATGAGCTG 
TTTCGTGATC GCTACAACCA CACCTTCTGC TTTGAGAAGT TCCCCATGGA GCGTTGGGTG 
GCCTGGATGA ATCTGTACCG CGTCTTTGTG GGCTTCCTCT TCCCCTGGGC ACTCATGTTG 
CTGTGCTACC GTGGCATCCT GAGGGCAGTG CAGAGCAGTG TGTCCACCGA GCGCCAGGAG 
AAAGTCAAGA TCAAACGTCT GGCCCTGAGC CTCATCGCCA TTGTGCTGGT GTGCTTTGCG 
CCTTACCATG CTCTCCTGCT GTCTCGCAGC GCCGTCTACC TGGGCCGGCC CTGGGACTGT 
GGCTTCGAGG AGCGAGTCTT TTCTGCCTAC CACAGCTCCC TGGCCTTCAC CAGCCTCAAT 
TGTGTGGCTG ACCCCATCCT CTACTGCCTG GTCAACGAGG GTGCCCGCAG TGATGTGGCC 
AAGGCCCTGC ACAACCTCCT CCGCTTCCTG GCCAGCAACA AGCCCCAGGA GATGGCCAAT 
GCTTCCCTCA CCCTGGAGAC ACCCTTGACC TCCAAGAGGA GCACCACCGG CAAGTCGTCC 
GGGGCTGTCT GGGCAGTGCC TCCGACTGCC CAGGGGGACC AGGTGCCACT GAAGGTGCTG 
CTGCCCCCGG CACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540111.1
Translation	MDNSTGTGEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVRQHNELGVYLMNLSIADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYSNIYISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMERWVAWMNLYRVFVGFLFPWALMLLCYRGILRAVQSSVSTERQEKVKIKRLALSLIAIVLVCFAPYHALLLSRSAVYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASNKPQEMANASLTLETPLTSKRSTTGKSSGAVWAVPPTAQGDQVPLKVLLPPAQ

Target ORF information:

RefSeq Version	XM_006540048.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus G protein-coupled receptor 4 (Gpr4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540048.2

ORF Insert Sequence:

ATGGACAACA GCACGGGCAC AGGGGAGGGC TGCCATGTGG ACTCTCGAGT GGACCACCTC 
TTCCCACCAT CTCTCTACAT CTTCGTCATC GGGGTGGGGC TGCCCACCAA CTGCCTGGCC 
CTGTGGGCAG CCTACCGGCA GGTGCGCCAA CACAATGAGC TGGGCGTCTA CCTGATGAAC 
TTGAGCATTG CAGACCTGCT GTACATCTGC ACTTTGCCGC TGTGGGTCGA CTACTTCCTC 
CACCATGACA ACTGGATCCA CGGCCCTGGC TCCTGCAAGC TCTTTGGCTT CATCTTCTAC 
AGCAACATCT ATATCAGCAT CGCCTTCCTG TGCTGCATCT CCGTGGACCG CTACCTGGCT 
GTGGCTCATC CTCTGCGCTT TGCACGCCTG CGCCGGGTCA AGACAGCAGT GGCTGTGAGC 
TCTGTGGTCT GGGCCACGGA GCTGGGCGCC AATTCAGCAC CGCTCTTCCA TGATGAGCTG 
TTTCGTGATC GCTACAACCA CACCTTCTGC TTTGAGAAGT TCCCCATGGA GCGTTGGGTG 
GCCTGGATGA ATCTGTACCG CGTCTTTGTG GGCTTCCTCT TCCCCTGGGC ACTCATGTTG 
CTGTGCTACC GTGGCATCCT GAGGGCAGTG CAGAGCAGTG TGTCCACCGA GCGCCAGGAG 
AAAGTCAAGA TCAAACGTCT GGCCCTGAGC CTCATCGCCA TTGTGCTGGT GTGCTTTGCG 
CCTTACCATG CTCTCCTGCT GTCTCGCAGC GCCGTCTACC TGGGCCGGCC CTGGGACTGT 
GGCTTCGAGG AGCGAGTCTT TTCTGCCTAC CACAGCTCCC TGGCCTTCAC CAGCCTCAAT 
TGTGTGGCTG ACCCCATCCT CTACTGCCTG GTCAACGAGG GTGCCCGCAG TGATGTGGCC 
AAGGCCCTGC ACAACCTCCT CCGCTTCCTG GCCAGCAACA AGCCCCAGGA GATGGCCAAT 
GCTTCCCTCA CCCTGGAGAC ACCCTTGACC TCCAAGAGGA GCACCACCGG CAAGTCGTCC 
GGGGCTGTCT GGGCAGTGCC TCCGACTGCC CAGGGGGACC AGGTGCCACT GAAGGTGCTG 
CTGCCCCCGG CACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr4 ( XM_006540048.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540111.1 Mus musculus G protein-coupled receptor 4 (Gpr4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu05694
Clone ID Related Accession (Same CDS sequence)	XM_006540048.2 , XM_006540048.3 , NM_175668.4
Accession Version	XM_006540048.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	G-protein coupled receptor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540048.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACA GCACGGGCAC AGGGGAGGGC TGCCATGTGG ACTCTCGAGT GGACCACCTC 
TTCCCACCAT CTCTCTACAT CTTCGTCATC GGGGTGGGGC TGCCCACCAA CTGCCTGGCC 
CTGTGGGCAG CCTACCGGCA GGTGCGCCAA CACAATGAGC TGGGCGTCTA CCTGATGAAC 
TTGAGCATTG CAGACCTGCT GTACATCTGC ACTTTGCCGC TGTGGGTCGA CTACTTCCTC 
CACCATGACA ACTGGATCCA CGGCCCTGGC TCCTGCAAGC TCTTTGGCTT CATCTTCTAC 
AGCAACATCT ATATCAGCAT CGCCTTCCTG TGCTGCATCT CCGTGGACCG CTACCTGGCT 
GTGGCTCATC CTCTGCGCTT TGCACGCCTG CGCCGGGTCA AGACAGCAGT GGCTGTGAGC 
TCTGTGGTCT GGGCCACGGA GCTGGGCGCC AATTCAGCAC CGCTCTTCCA TGATGAGCTG 
TTTCGTGATC GCTACAACCA CACCTTCTGC TTTGAGAAGT TCCCCATGGA GCGTTGGGTG 
GCCTGGATGA ATCTGTACCG CGTCTTTGTG GGCTTCCTCT TCCCCTGGGC ACTCATGTTG 
CTGTGCTACC GTGGCATCCT GAGGGCAGTG CAGAGCAGTG TGTCCACCGA GCGCCAGGAG 
AAAGTCAAGA TCAAACGTCT GGCCCTGAGC CTCATCGCCA TTGTGCTGGT GTGCTTTGCG 
CCTTACCATG CTCTCCTGCT GTCTCGCAGC GCCGTCTACC TGGGCCGGCC CTGGGACTGT 
GGCTTCGAGG AGCGAGTCTT TTCTGCCTAC CACAGCTCCC TGGCCTTCAC CAGCCTCAAT 
TGTGTGGCTG ACCCCATCCT CTACTGCCTG GTCAACGAGG GTGCCCGCAG TGATGTGGCC 
AAGGCCCTGC ACAACCTCCT CCGCTTCCTG GCCAGCAACA AGCCCCAGGA GATGGCCAAT 
GCTTCCCTCA CCCTGGAGAC ACCCTTGACC TCCAAGAGGA GCACCACCGG CAAGTCGTCC 
GGGGCTGTCT GGGCAGTGCC TCCGACTGCC CAGGGGGACC AGGTGCCACT GAAGGTGCTG 
CTGCCCCCGG CACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540111.1
CDS	1010..2107
Translation	MDNSTGTGEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVRQHNELGVYLMNLSIADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYSNIYISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMERWVAWMNLYRVFVGFLFPWALMLLCYRGILRAVQSSVSTERQEKVKIKRLALSLIAIVLVCFAPYHALLLSRSAVYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASNKPQEMANASLTLETPLTSKRSTTGKSSGAVWAVPPTAQGDQVPLKVLLPPAQ

Target ORF information:

RefSeq Version	XM_006540048.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 4 (Gpr4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540048.3

ORF Insert Sequence:

ATGGACAACA GCACGGGCAC AGGGGAGGGC TGCCATGTGG ACTCTCGAGT GGACCACCTC 
TTCCCACCAT CTCTCTACAT CTTCGTCATC GGGGTGGGGC TGCCCACCAA CTGCCTGGCC 
CTGTGGGCAG CCTACCGGCA GGTGCGCCAA CACAATGAGC TGGGCGTCTA CCTGATGAAC 
TTGAGCATTG CAGACCTGCT GTACATCTGC ACTTTGCCGC TGTGGGTCGA CTACTTCCTC 
CACCATGACA ACTGGATCCA CGGCCCTGGC TCCTGCAAGC TCTTTGGCTT CATCTTCTAC 
AGCAACATCT ATATCAGCAT CGCCTTCCTG TGCTGCATCT CCGTGGACCG CTACCTGGCT 
GTGGCTCATC CTCTGCGCTT TGCACGCCTG CGCCGGGTCA AGACAGCAGT GGCTGTGAGC 
TCTGTGGTCT GGGCCACGGA GCTGGGCGCC AATTCAGCAC CGCTCTTCCA TGATGAGCTG 
TTTCGTGATC GCTACAACCA CACCTTCTGC TTTGAGAAGT TCCCCATGGA GCGTTGGGTG 
GCCTGGATGA ATCTGTACCG CGTCTTTGTG GGCTTCCTCT TCCCCTGGGC ACTCATGTTG 
CTGTGCTACC GTGGCATCCT GAGGGCAGTG CAGAGCAGTG TGTCCACCGA GCGCCAGGAG 
AAAGTCAAGA TCAAACGTCT GGCCCTGAGC CTCATCGCCA TTGTGCTGGT GTGCTTTGCG 
CCTTACCATG CTCTCCTGCT GTCTCGCAGC GCCGTCTACC TGGGCCGGCC CTGGGACTGT 
GGCTTCGAGG AGCGAGTCTT TTCTGCCTAC CACAGCTCCC TGGCCTTCAC CAGCCTCAAT 
TGTGTGGCTG ACCCCATCCT CTACTGCCTG GTCAACGAGG GTGCCCGCAG TGATGTGGCC 
AAGGCCCTGC ACAACCTCCT CCGCTTCCTG GCCAGCAACA AGCCCCAGGA GATGGCCAAT 
GCTTCCCTCA CCCTGGAGAC ACCCTTGACC TCCAAGAGGA GCACCACCGG CAAGTCGTCC 
GGGGCTGTCT GGGCAGTGCC TCCGACTGCC CAGGGGGACC AGGTGCCACT GAAGGTGCTG 
CTGCCCCCGG CACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GPR4 knockout improves renal ischemia-reperfusion injury and inhibits apoptosis via suppressing the expression of CHOP.
The Biochemical journal474(24)4065-4074(2017 12)
Dong B,Zhang X,Fan Y,Cao S,Zhang X

GPR4 deficiency alleviates intestinal inflammation in a mouse model of acute experimental colitis.
Biochimica et biophysica acta1863(2)569-584(2017 Feb)
Sanderlin EJ,Leffler NR,Lertpiriyapong K,Cai Q,Hong H,Bakthavatchalu V,Fox JG,Oswald JZ,Justus CR,Krewson EA,O'Rourke D,Yang LV

Deletion of proton-sensing receptor GPR4 associates with lower blood pressure and lower binding of angiotensin II receptor in SFO.
American journal of physiology. Renal physiology311(6)F1260-F1266(2016 Dec)
Sun X,Tommasi E,Molina D,Sah R,Brosnihan KB,Diz D,Petrovic S

Acidic environment augments Fc?RI-mediated production of IL-6 and IL-13 in mast cells.
Biochemical and biophysical research communications464(3)949-55(2015 Aug)
Kamide Y,Ishizuka T,Tobo M,Tsurumaki H,Aoki H,Mogi C,Nakakura T,Yatomi M,Ono A,Koga Y,Sato K,Hisada T,Dobashi K,Yamada M,Okajima F

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Gpr4 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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